LOCUS       DATBRK010000001         4038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_433345, whole genome shotgun sequence.
ACCESSION   DATBRK010000001 DATBRK010000000
VERSION     DATBRK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433345"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VIH70_00005"
     CDS             <1..463
                     /locus_tag="VIH70_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8114778.1"
                     /translation="ATMQVRDIMHTDVKTTSPSDTFEQVATVLREHGISSVIVMDGDT
                     LAGIVTERDLVNLVAEGGDPKATTVSERMSTDLDTVDPKTDIAEAADHMARLKIRHLP
                     VLQDGQLAGIISIRDLTNWAVHELTSGHELPDLERSHSALSAAAEVNRPGP"
     gene            complement(444..1757)
                     /locus_tag="VIH70_00010"
     CDS             complement(444..1757)
                     /locus_tag="VIH70_00010"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8114779.1"
                     /translation="MTGKRILVVGAGFAGLYAALHLRRAVDLGHRVTVVNPENFMQYH
                     PFLPEVASGTIDPRAAVVPLRRVLRHCEVVIGEVERVDRGERAARVRLADGRQVALGY
                     DVVVLAPGSWARVLPIPGLAAHGVGFKTVQEAIWLRNHVLSRLDLAAVSEDAERRRAT
                     LTFVFVGGGYAGVEALGELEDLARDAIESYPSLRPEQMRWVLVEATGSILPEIGPSLA
                     DYAIERLRKRGIEVLLNTRLDSAEDGVLRLSDGQAFPAETLVWTAGVKPSPLAAESGF
                     PVDETGRIKTDAFLRVEGVGSAWAAGDSAAVPDLLGGGLMPPTAQHGMRQGKRLGSNV
                     LAVLEGRPPERFRYRGIGAVVSLGRYKGVATIRGVRLRGFPAWFAHRSYHLYAMPTLG
                     RKVKIAADWTVALLFARDLAQLGSLEHPREPFERAARARPTDPGD"
     gene            complement(1769..2626)
                     /locus_tag="VIH70_00015"
     CDS             complement(1769..2626)
                     /locus_tag="VIH70_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019354410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY8114780.1"
                     /translation="MTRIDLHTHSTHSDGTFEPAEVIRLGAEGDLDVVALTDHDTTDG
                     LPEALATGFDIGVEVVPGVEFSAEYLGTSVHILCYWMDEEDPALQLELRRLREDRYRR
                     GELMVEKLGSLGLPVAFERVREIAGDATIVRPHIAQAMVEAGIVETEKEAFERYIGDG
                     RPAHVAKHALDPIDAVALIEGAGGVCVLAHPGMWGDQSSVPDELIERMAASGMRGLEV
                     DHPDHTPEARERYRALAGRLGLIATGGSDCHGTRFDPVRLGGTLCDPEAFAALRAAAF
                     RGSPAAPSP"
     gene            complement(2662..3678)
                     /locus_tag="VIH70_00020"
     CDS             complement(2662..3678)
                     /locus_tag="VIH70_00020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY8114781.1"
                     /translation="MDAPTILTVDDYEPLARQVLPADVFDYFAGGAGDEWTLAENRRA
                     FERWVIRPRMLTGAWPVDPSTRILGTDIAFPVLVAPWAYQKRAHPEGELATARAAARA
                     GTVMVVSTTTVDYLEEIAAASGAPKWWQLYVFTDRGLTVEMLHRVHAARFGAVCLTVD
                     FPDSGLRHRDARNLFDMPIGLPQDDLTFAPDLTWDDLAWIREAAPVPLLVKGLLTAED
                     ALLAVEAGVDAIVVSNHGGRQLDSVHAPITVLPEVVEAVEGRVPVLVDGGFRRGTDVF
                     KALALGADAVLVARPACWGLAVAGEDGVVDVLRILRAELENTMVLAGTRSVADISGAL
                     VERA"
     gene            3790..>4038
                     /locus_tag="VIH70_00025"
     CDS             3790..>4038
                     /locus_tag="VIH70_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY8114782.1"
                     /translation="MTTAADLRAAAFLKVVTWARVALVPVILALILAGPDSETAFTIA
                     AALFAVAALTDFFDGLLARRWAQTSVFGNFLDTTADKLL"
BASE COUNT          603 a         1503 c         1408 g          524 t
ORIGIN      
        1 agccacgatg caggtccgcg acatcatgca caccgacgtg aagaccacct cgccctcgga
       61 cacgttcgag caggtggcga ccgtgctgcg cgagcacggg atctcctcgg tcatcgtcat
      121 ggacggcgac accctcgccg gcatcgtgac cgagcgcgac ctggtcaacc tcgtggccga
      181 aggtggcgac ccgaaggcga cgacggtctc cgagcgcatg tcgaccgatc tggacacggt
      241 cgacccgaag accgacatcg ccgaggcggc cgaccacatg gctcggctga agatccgtca
      301 cctgcccgtc ctgcaggacg ggcagctcgc ggggatcatc tcgatccgcg acctgaccaa
      361 ctgggccgtg cacgagctca cctcgggtca cgagctcccc gacctcgagc ggagccactc
      421 ggcgctctcg gccgccgccg aggtcaatcg cccgggtccg taggccgcgc cctcgcggcg
      481 cgctcgaacg gctcgcgcgg gtgctccagc gagccgagct gtgcgagatc gcgggcgaac
      541 aggagcgcga ccgtccagtc ggccgcgatc ttgaccttcc tgccgagcgt cggcatcgcg
      601 tacaggtgat acgagcggtg cgcgaaccac gccgggaacc ctcggaggcg gacgccccgg
      661 atcgtcgcca cgcccttgta ccggccgagc gagacgacgg ccccgatccc tcggtatcgg
      721 aaccgttcgg gcgggcgccc ctccaacacc gccaggacgt tgctccccag ccgcttcccc
      781 tgtcgcatcc cgtgctgcgc ggtgggcggc atcagccccc cgccgaggag atccggcacc
      841 gccgccgagt cgccggccgc ccacgcgctc ccgacgccct cgacccgcag gaacgcgtcg
      901 gtcttgatgc ggcccgtctc gtcgaccggg aagccggact cggccgcgag gggtgacggc
      961 ttgacgccgg cggtccacac gagcgtctcg gccgggaagg cctgcccgtc ggacaggcgg
     1021 agcaccccgt cctccgcgga gtcgagccgc gtgttcagca ggacctcgat cccgcgcttc
     1081 cgcaggcgct cgatcgcgta gtcggcgagg ctcgggccga tctcgggcag gatcgagccg
     1141 gtcgcctcga ccaggaccca ccgcatctgc tcgggtcgca ggctcgggta gctctcgatc
     1201 gcgtcgcggg ccaggtcctc gagctcgccc aacgcctcga cccccgcgta ccccccgccg
     1261 acgaacacga acgtgagcgt cgcccgccgg cgttccgcgt cctcgctcac cgcggcgagg
     1321 tcgagccgcg agagcacgtg gttgcgcagc cagatggcct cctggaccgt cttgaacccg
     1381 accccgtgcg cggccaggcc ggggatgggg agcacgcgcg cccacgagcc gggcgcgagc
     1441 acgaccacgt cgtagccgag ggccacctgc cggccgtcgg cgaggcgaac tcgcgcggcc
     1501 cgctcgccac ggtcgacgcg ctccacctcg ccgatcacga cctcgcagtg acggagcacc
     1561 cgccggagcg ggacgaccgc cgcccgcgga tcgatcgtgc ccgacgcgac ctcgggcagg
     1621 aacgggtggt actgcatgaa gttctcgggg ttgacgaccg tcacccggtg accgaggtcg
     1681 accgctcggc gcagatgcag ggcggcgtag agcccggcga acccggcgcc cacgacgagg
     1741 atccgcttgc cggtcatacg ggccgatgct acggggaggg cgccgcgggc gatccgcgga
     1801 acgccgcggc gcgcagcgcg gcgaacgcct ccggatcgca gagcgtgccc cccagccgga
     1861 cggggtcgaa ccgcgtcccg tggcagtccg agccgccggt cgcgatcagg ccgagccggc
     1921 ccgccagggc gcggtagcgt tcgcgagcct cgggggtgtg atcgggatgg tcgacctcga
     1981 gaccccgcat gccggacgcc gccatgcgct cgatcaactc gtccggcacc gagctctggt
     2041 cgccccacat ccccgggtgc gcgagcacgc acacaccgcc cgccccctcg atgagggcca
     2101 cggcgtcgat cgggtcgagc gcgtgcttgg cgacgtgcgc cggccggccg tcgccgatgt
     2161 agcgctcgaa cgcctccttc tcggtctcga cgatccccgc ctcgaccatc gcctgcgcga
     2221 tgtgcgggcg gacgatcgtg gcgtccccgg cgatctcgcg gacgcgttcg aacgcgaccg
     2281 gcagaccgag ggaaccgagc ttctcgacca tcagctcgcc gcgccggtag cggtcctcgc
     2341 gcagccgccg gagctcgagc tgcagcgcgg ggtcctcctc gtccatccag tagcacagga
     2401 tgtggacgct cgtccccaga tactcggcgg agaactcgac gccgggcacg acctcgacgc
     2461 cgatatcgaa cccggtggcg agcgcctcgg gcaggccgtc cgtcgtgtcg tgatccgtca
     2521 gcgcgaccac gtccaggtcg ccttcggcgc cgaggcggat cacctcggcg ggctcgaagg
     2581 tcccgtcgga gtgggtcgaa tgcgtgtgca ggtcgatgcg cgtcatgcgg acgccatgct
     2641 ggcagacgac ctcgaaccgc gtcacgcgcg ttcgacgagc gcccccgaga tgtcggcgac
     2701 cgaacgcgtg ccggccagga ccatcgtgtt ctcgagctcg gcccgcagga tgcgaaggac
     2761 gtccaccacg ccgtcctccc ccgccaccgc gaggccccaa cacgcggggc gcgcgaccag
     2821 cacggcgtcc gcgccgagcg cgagcgcctt gaagacgtcc gtcccgcgtc tgaacccgcc
     2881 gtcgacgagc accggcacgc gaccctcgac ggcctcgacg acctccggga gcacggtgat
     2941 cggcgcgtgg accgaatcga gctggcggcc gccgtggttc gagacgacga tcgcgtcgac
     3001 gccggcctcg acggcgagca gcgcgtcctc ggcggtcagg agccccttca cgagcagggg
     3061 caccggagcg gcctcccgga tccacgcgag atcgtcccag gtgaggtcgg gagcgaacgt
     3121 gagatcgtcc tggggcaaac cgatcggcat gtcgaagagg tttcgcgcat cccgatggcg
     3181 caggccgctg tcgggaaagt ccaccgtcag gcacacggcc ccgaacctcg ccgcatgcac
     3241 ccggtgcagc atctcgaccg tcaggccccg atcggtgaag acgtacagct gccaccactt
     3301 cggggcgccc gaagcggccg cgatctcttc gaggtagtcg accgtggtgg tcgagaccac
     3361 catgacggtc ccggcccgag cggcggcccg cgcggtggcg agctctccct cgggatgcgc
     3421 gcgcttctgg taggcccacg gcgcgacgag gaccgggaac gcgatgtcgg ttccgaggat
     3481 ccgggtggag ggatcgaccg gccaggcgcc ggtgagcatc cgcggccgga tcacccagcg
     3541 ctcgaacgcg cgacggttct ccgccagcgt ccactcgtcc cccgcgccgc cggcgaagta
     3601 gtcgaagacg tcggccggca ggacctgtct ggcgagcggc tcgtagtcgt cgaccgtgag
     3661 gatcgtcggc gcgtccatgc agcgcagcct agtcagtcag ggctgcgagg ggaccggcgg
     3721 tgacgacgcc gacccggaag cccgtcgggc gttcgcggcg aggcgcgctg ctacgatgcc
     3781 gacgaagccg tgacgaccgc cgccgacctc cgcgccgcag cgttcctcaa ggtcgtgacg
     3841 tgggctcggg tggcgctcgt cccggtgatc ctggcgctga tcctcgccgg accggacagc
     3901 gaaacggcgt tcaccatcgc cgcggcgctg ttcgcggtcg ccgcgctcac cgacttcttc
     3961 gacgggctgc tggcccgccg ctgggcgcag acgagcgtgt tcggcaactt cctcgacacg
     4021 acggcggaca agttgctg
//
LOCUS       DATBRK010000002         5152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127 k141_142,
            whole genome shotgun sequence.
ACCESSION   DATBRK010000002 DATBRK010000000
VERSION     DATBRK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2293
                     /locus_tag="VIH70_00030"
     CDS             <1..2293
                     /locus_tag="VIH70_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8114783.1"
                     /translation="VTFGPLAQILLHEMGEAAHAPPEALRARLEEIVLECTPPDEARQ
                     IVERLCIALGIAGEARDEKRYRVAEIRSGLLALLRGFSSRGPVVVVLEDLQAAQAPML
                     ELVEELVREADGVPLLVICAARYTLLDDRPGWGGGRGNSVNLYLETLSLDDSAQLARD
                     AGEGLDEATAQRIARHAGGNPFFIIETTGMLLHQGKGFSADTGPLPPALLPPTVQAVI
                     AARIDHLSPEGRDLIRKASVFARSSFTISELDLIAVPTNEALVRLEEEELLERDGDRP
                     DVWRFSHGMVREVAYESLPKRERQRLHLLVAEGLSGNPETAARRPRAIAFHLEQAALA
                     SLDLNPRDRTLAERAVVALARAGDIAREGPNMKAAEEDYLRALELAGPERAWGLREAR
                     ILASLGEIRYWLGEFEPAVPVLERALRLGDGDLAVRAQASRFLGDIELSILGDAKRAA
                     ELLDDALDAARGLEDPWTLARTLLVAAWAPYFRRDVDTARAMFQEALETARANPEGDR
                     WSEARSLVGLSTLESDGGDEEGSHRLAAEALVVAETSGDRFSIAVAHEAVGGTMRHMM
                     RLEDAEEHMDRAVDAFRGLGARWELASALTSRGIARRLAGEADEAVTDLLEAYRICRE
                     LKERSIITWTAAALARAYVGAGDPGAARRVLEETATVASEETAATEEWLDYADVEILV
                     AEGEREAALERALSILRYERGEGSSKDVAARVWFISQVFGVEAAGGEEEVGRARELLE
                     RTQMLQAFREAELITSRSAISGA"
     gene            2311..3468
                     /locus_tag="VIH70_00035"
     CDS             2311..3468
                     /locus_tag="VIH70_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8114784.1"
                     /translation="MDFDLTPEQDELRSAVRAFAEDVVAPAAAEADEREEFPTEIVKR
                     AAELGLFGIPFPEEVGGQGGDLLSFCLCLEEIGRYDSALAITLEAAVGLAANPLFRFG
                     TDEQQQRWLVPMARGEAIGAFALTEPGGGSDARAIRTTAKLDGDEWVIDGSKAFITNS
                     GTPLTSVIILAAVDEAADDGDVSTIVVPVGTPGLTVGASYRKVGWRASDTHEVSFDAC
                     RVPTDHLLGERGRGYSQFLETLADGRVAIAALSVGLARGCLEESARYAGEREAFGQPI
                     GAHQALQFKIADMRVGVETARLAYQRAAWLRDRGRPFATEASIAKLYASEVAVSSARE
                     AVQVHGGYGFIEDFPVARFYRDAKVLEIGEGTSEIQRVLIARALGLPGTEL"
     gene            3599..4759
                     /locus_tag="VIH70_00040"
     CDS             3599..4759
                     /locus_tag="VIH70_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8114785.1"
                     /translation="MAENHSGSGVVFHRALAAVAALALLTSLAQVAAAEPSTEERLDA
                     AKAEFEQLKNQIASQQQVLNGLSAEAAVIAERWEVATGRWEQVTAELHQTQFALAEAQ
                     AEYQGLKSDLEERAREAYITGPGSGLEFLLGASSIADLSARMEYVNALSQEDADLATE
                     VQNVRNTLAAQKEDQEKLQAKRAKAVKKVESERAALDAKFDEQQAVLSDLNSAKARAE
                     DLVRQLTKRYQNELEALTGLEFHGGTVFAVCPVDQPRAVYDGFGAPRYGGGYHPHAGN
                     DIIAPQGTPIRATFDGYAQAGSNTLGGYSVNVYGDLGYTYNAHLMQPGVTGQVSAGQV
                     VGYVGATGDTSTPHDHFEWHPNVIPSDWPVSPYGYNIIGSAVNPFPLLAQVC"
     gene            complement(4747..>5152)
                     /locus_tag="VIH70_00045"
     CDS             complement(4747..>5152)
                     /locus_tag="VIH70_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114786.1"
                     /translation="YLVEATYQPSGEAARLANPASVVILYPLLANDHGTHSIIWSRSG
                     ERWKALDTDDLPSIQQAGADIDELGYLSVAGQPASPTAGSGTGGGSSVATIAIVAGLI
                     VLAVVAAVLLRRGSRPRDRELEQRRRRRRQQT"
BASE COUNT          762 a         1757 c         1928 g          705 t
ORIGIN      
        1 cgtgaccttc ggcccgctcg cgcagatcct cctgcacgag atgggggagg cggcgcacgc
       61 acctcccgag gcgctccgcg cgcggctcga ggagatcgtc ctggagtgca ccccgcccga
      121 cgaggcgagg cagatcgtcg agcgcctgtg catcgcgctc gggatcgccg gcgaggcgcg
      181 cgacgagaag cgctaccgag tggccgagat ccgctcgggg ctgctcgcgc tcctgcgcgg
      241 gttctccagc cggggaccgg tggtcgtggt gctcgaggac ctccaggcgg cccaggcgcc
      301 gatgctcgag ctggtcgagg agttggtgcg cgaggccgac ggcgtcccgt tgctcgtgat
      361 ctgcgccgcc cgatacacgc tgctggacga ccgccccggc tggggcggcg ggcgcgggaa
      421 ctcggtcaac ctctacctgg agacgctgtc gctcgacgac tctgcgcaac tggcgcggga
      481 cgccggcgaa ggcctggacg aggcgaccgc ccagcggatc gcgcggcatg ccggcgggaa
      541 cccgttcttc atcatcgaga cgaccgggat gctgctgcat caaggaaagg ggttctcggc
      601 cgacacgggg ccgctgccgc cggcgctgct gccgccgacc gtacaagccg tgatcgccgc
      661 ccggatcgat cacctctcac ccgagggccg agatctcatc cgaaaggcct cggtgttcgc
      721 ccgctcgtcg ttcaccatct ccgagctcga cctgatcgcc gtcccgacca acgaggcgct
      781 cgtccggctc gaggaggagg agctgctcga acgcgacggc gatcgccccg acgtctggcg
      841 cttcagccac gggatggtgc gcgaggtcgc ctacgagagc ctccccaagc gcgagcgcca
      901 gcgcctgcac ctgttggtcg ccgaaggcct gtctggcaac ccggagacgg ccgcccgccg
      961 cccgcgcgcg atcgcgttcc atctggaaca agcggcgctc gcctcgctcg acctcaaccc
     1021 gcgcgaccga acgctcgccg aacgtgcggt ggtggccctg gctcgcgccg gcgacatcgc
     1081 gcgcgagggg ccgaacatga aggcggccga ggaggactac ctccgcgcgc tggagctcgc
     1141 cggccccgag cgcgcgtggg ggctccgcga ggcccggatc ctcgcgagcc tcggcgagat
     1201 ccgttactgg ctcggcgagt tcgagcccgc cgttccggtg ctggaacgcg cgctcaggct
     1261 cggcgacggc gacctcgcgg tccgggcgca ggcctcgcgg ttcctcggcg acatcgagct
     1321 ctcgatcctt ggcgacgcca agcgcgcggc cgagctgctc gacgatgctc tcgacgcggc
     1381 gcgcgggctc gaggatccgt ggacgctagc gcggaccctg ctcgtcgcgg cgtgggcgcc
     1441 gtatttccgg cgggacgtcg acacggcgcg cgcgatgttc caggaggcgc tcgagaccgc
     1501 acgcgcgaac ccggagggcg accggtggtc cgaggctcgg tcgctcgttg ggctctccac
     1561 gctcgagagc gacgggggcg acgaggaggg ctcgcaccgg ctcgcggccg aggcgctcgt
     1621 cgtcgccgag acgagcgggg accgcttctc gatcgcggtc gcacacgagg cggttggcgg
     1681 cacgatgcgc cacatgatgc ggctggagga cgccgaggag cacatggatc gcgcggtcga
     1741 cgccttccgc gggctcggcg cgcgttggga gctcgcgagc gcgctcacgt cgcggggcat
     1801 cgcgcgacgg ctggccggcg aggctgacga ggcggtgacc gacctgctcg aggcctaccg
     1861 aatctgccgt gagctgaagg agcggagcat catcacctgg acggccgccg cgctcgccag
     1921 ggcgtacgtc ggcgcgggcg atccgggagc ggcccgccgt gtcctggagg agaccgcgac
     1981 ggtcgcctcc gaggagaccg ccgccaccga ggagtggctc gactacgccg acgtcgagat
     2041 cctggtggcc gagggcgagc gggaggcggc gttggagcgc gctctctcga tcttgcgcta
     2101 cgagcgtgga gaggggtcct ccaaggacgt ggccgcacgc gtgtggttca tctcgcaggt
     2161 gttcggcgtc gaggccgccg ggggcgagga ggaggtcggg cgcgccaggg agctgctcga
     2221 acgcacccag atgctccagg ccttccgaga ggccgagctg atcacgagcc gttcggcgat
     2281 ctcgggggcc tgaccgctag gcttcgaccc gtggacttcg atctcacccc ggagcaggac
     2341 gagctgcgat ccgcggtgcg cgcgttcgcc gaggacgtgg tggcgccggc cgcggccgag
     2401 gccgacgagc gcgaggagtt ccccaccgag atcgtcaagc gcgccgccga gctcgggctg
     2461 ttcggcatcc cgttccccga ggaggtcggc ggacaggggg gcgacctgct ctcgttctgc
     2521 ctgtgtctcg aggagatcgg gcggtacgac tcggcgctcg cgatcacgct cgaggccgcg
     2581 gtgggcctgg ccgcgaaccc tctgttccgg ttcggcaccg atgagcagca gcagcgatgg
     2641 ctcgtgccga tggcacgagg cgaggcgatc ggcgcgttcg cgctcaccga acccggtgga
     2701 ggctcggatg ctcgtgcgat ccggacgacg gccaagctgg acggcgacga gtgggtgatc
     2761 gacggctcga aggcgttcat caccaactcg gggacgccgc tgacgtcggt gatcatcctg
     2821 gcggcggtcg acgaggcggc ggacgacggc gacgtctcga cgatcgtcgt gccggtcggg
     2881 acgcccgggc tcacggtcgg cgcgagctac cgcaaggtcg gttggcgcgc gagcgacacg
     2941 cacgaggtct cgttcgacgc gtgccgcgtg ccgacggacc atctgctcgg cgagcgcggc
     3001 cgtgggtatt cccagttcct cgagacgctc gccgacgggc gggtcgcgat cgcggcgctc
     3061 tcggtcgggc tcgcgcgtgg ctgcttggag gagagcgccc ggtacgcggg ggagcgggag
     3121 gcgttcgggc aaccgatcgg cgcgcaccag gcgctgcagt tcaagatcgc cgacatgcgg
     3181 gtgggggtcg agacggcgcg gctcgcgtac cagcgggcgg cgtggctccg cgaccgagga
     3241 cgccccttcg cgaccgaggc gtcgatcgcc aagctctacg cgagcgaggt cgcggtctcg
     3301 agcgcgcgag aagcggtgca ggtccacggc ggctacggct tcatcgagga ctttcccgtg
     3361 gcccgcttct accgcgacgc caaggtgctc gagatcgggg agggcaccag cgagatccag
     3421 cgcgtgctca tcgcccgggc gctcggcctg cccggaaccg agctgtaggc ctcgcccgac
     3481 ggggtccgcc cgcgtcccgt atgggcggaa aagccccgcc gaggatggtt gcaaccgcgg
     3541 gctgcagcgg gcacggttga catcccactc ggccgtccga taggaagggc cttccccaat
     3601 ggccgaaaac cacagcggtt caggcgtcgt gttccaccgc gccctcgcgg cggtggcggc
     3661 gctggccctc ctgacgtcgc tcgcccaggt cgcggccgcc gagccgagca ccgaggagag
     3721 gctcgacgcc gccaaggccg agttcgagca gctgaagaac cagatcgcga gccagcagca
     3781 ggtcctgaac gggctctcgg cggaggcggc cgtgatcgcc gagcgctggg aggtcgcgac
     3841 cggccgctgg gagcaggtca ccgccgagct gcaccagacg cagttcgccc tcgccgaggc
     3901 gcaggcggag taccagggcc tcaaatcaga cctggaggag cgcgcccgcg aggcctacat
     3961 caccggcccg gggtcgggtc tcgagttcct gctgggcgcg tcgtccatcg cggacctgtc
     4021 ggcgcgcatg gagtacgtga acgccctgag ccaggaggac gccgaccttg cgacggaggt
     4081 gcagaacgtt cggaacaccc tcgcggcgca gaaggaagac caggagaagc tgcaggcgaa
     4141 gcgggcgaag gccgtcaaga aggtcgagag cgaacgggcc gcgctcgatg cgaagttcga
     4201 cgagcagcag gcggtgctca gcgacctgaa ttcggcgaaa gcgcgtgccg aggacctggt
     4261 gaggcaactc accaagcgct accagaacga gctcgaggcc ctgaccggcc tcgagttcca
     4321 cggaggcacc gtcttcgcgg tgtgcccggt cgatcagccg cgggccgtgt acgacgggtt
     4381 cggcgcgccg cggtacgggg gcgggtacca cccgcacgcg ggcaacgaca tcatcgcgcc
     4441 gcagggcacg ccgatccgag cgacgttcga cgggtacgcc caggcgggct cgaacacgct
     4501 cggtgggtac tcggtcaacg tctacgggga cctgggctac acgtacaacg cgcacctcat
     4561 gcaaccgggt gtcacggggc aggtgtcggc cgggcaggtc gtcggttacg tgggcgcgac
     4621 cggcgacacg tcgacgccgc acgatcactt cgagtggcac cccaacgtca tcccgtccga
     4681 ctggccggtg agcccgtacg gctacaacat catcgggtcg gcggtgaacc ccttcccgct
     4741 cctggctcag gtctgctgac ggcgccgccg gcgccgttgc tcgagctccc ggtctcgagg
     4801 ccgcgagccc cttcgcaaca gcaccgccgc gacgacggcg aggacgatca atccggcgac
     4861 gatcgcgatc gtggcgacgg acgacccgcc tccggtgccg gaccccgcgg tgggcgacgc
     4921 gggctgtccg gcgaccgaga ggtagccgag ctcgtcgatg tccgcgccgg cctgctggat
     4981 gctcggcagg tcgtcggtgt cgagcgcctt ccatcgttcg ccggacctcg accagatgat
     5041 cgaatgggtg ccgtggtcgt tcgcgagcag gggatagagg atgaccacgc tcgccgggtt
     5101 cgcgagcctc gcggcttcgc ccgagggttg gtaggtcgcc tcgaccaggt ag
//
LOCUS       DATBRK010000003         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1733264, whole genome shotgun sequence.
ACCESSION   DATBRK010000003 DATBRK010000000
VERSION     DATBRK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733264"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VIH70_00050"
     CDS             <1..295
                     /locus_tag="VIH70_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8114787.1"
                     /translation="VKEHGPDGARYFDGDDYIVLSIVKDFFRFGVEPRHLTMYKHFEE
                     READFFESIVMPQLRQRNPDARRGATASLVELARASRKLKQALLRTNLRQHLS"
     gene            352..1590
                     /locus_tag="VIH70_00055"
     CDS             352..1590
                     /locus_tag="VIH70_00055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY8114788.1"
                     /translation="MFALISVPLLSLGAARAQSPTPQPPPPTPVIQPGGQASLSPFPS
                     TLRTPATSTEVPEIHAASAVLADLDTGQVLFDLDRNERRPIASVTKIMTALLVVERAD
                     LTDAVTVSEGAASGPVSGISGLGLVAGERIRVDELLYALLLQSANDAAVALAEHVSGS
                     VDAFVDDMNVRAEQLGMTRTMFTSPNGLDDTGYSSAADLVRLTRAAYRSPGFASVVAT
                     RFHTVESLDAKPRIVQNRNVLLWLYPGAIGVKTGYTSPAGFCLVAAAQRGDERLVAVV
                     LGAPGEPFSAAAALLDYGFDAFERRTLLREGERLDDVVVGGRSLSVSAGESVRGLIPS
                     AATASRTVELEPHVAFPPARGEEIGIVRVSAGGRTVGEAPLVVVGVPGPPPPEAGSWW
                     SRAAGALVDATGGVLDALFG"
     gene            1613..2236
                     /gene="hpt"
                     /locus_tag="VIH70_00060"
     CDS             1613..2236
                     /gene="hpt"
                     /locus_tag="VIH70_00060"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY8114789.1"
                     /translation="MSGVVSVEFGREDIRRRIEELGRTITGDYEGRAPVLISVLKGGS
                     VFLADLMRAVSLPLSIDYMSISRYGGATESLGRVRIVKDLDQDIGGRDVIVVEDIVDT
                     GLTLAYLISVLRSRDPASVEVCALLDKSVRRIVPLELRYRGFECPDVFVVGYGLDFEE
                     RFRNVPDILALHQMEALRADPDLLIPYLPALDPAPSTGGFDPPEPSR"
     gene            2285..2764
                     /locus_tag="VIH70_00065"
     CDS             2285..2764
                     /locus_tag="VIH70_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEY8114790.1"
                     /translation="MIEMTVAGVRIEFPSNSPIVLLREAEGERFLPIWIGQSEAQAIA
                     IGQQGLVTPRPMTHDLLKNILDEMSVQVTSITITELRDGVFHAVIGLQRNGSSFEISS
                     RPSDAIALAVRLGCKIFANEDVLEEASILIPADGEEEEKQLQEFREFLENVTPEDFE"
     gene            complement(2770..3255)
                     /locus_tag="VIH70_00070"
     CDS             complement(2770..3255)
                     /locus_tag="VIH70_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY8114791.1"
                     /translation="MRLGTRTSKPRRTVVVTMSAVLALGIAFAPAARAGGGMRSKVLR
                     MVNATRNNHGLHTLRIDSSLSRDALRHTRHMIASNAIYDPTNLTRILQDEPWDVVGAS
                     AVGCADTLSALHNAFMHDPVHRAILLNSKIRRIGIGVIEVDSVNTCGRHWLWATELFY
                     G"
     gene            3470..3919
                     /locus_tag="VIH70_00075"
     CDS             3470..3919
                     /locus_tag="VIH70_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019126021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8114792.1"
                     /translation="MAEQGYRVPEVCNIVGITYRQLDYWARTGLVRPSVKDAKGSGSQ
                     RLYSFQDLAMLRLIKRMLDSGVNLQQVRKAMKTLKGLDQPALGTTLVSDGHRIYQVES
                     PEAVVDLLASGQGVFAIAVDKVWTDLEGTLAKNAGKKGRSARAAGGA"
     gene            3916..4287
                     /locus_tag="VIH70_00080"
     CDS             3916..4287
                     /locus_tag="VIH70_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114793.1"
                     /translation="MTGGAAAETPALPSDTVSFAHASERQFAQLLDFYQIEWDYEPRS
                     FDLEWDHDGNVVQRFTPDFYLPMYDLYIEITTLNQRLVTKKNRKVRRLRELYPEIKCK
                     IFYQRDYLSLVSKYGLEDVSG"
     gene            4334..>5010
                     /locus_tag="VIH70_00085"
     CDS             4334..>5010
                     /locus_tag="VIH70_00085"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HEY8114794.1"
                     /translation="MRFAPHTDDDVDQMLGAIGLSSVDELFDRIPRTVRLNRPLDLPD
                     GVSEMEIQADLAALAGRNRSAEDLVCFAGGGAYDHFVPAVVWALAGRSEFYTSYTPYQ
                     PELSQGVLQALFEYQSMICDLTGLEVSNASLYDGATALAEAVNLARSSPGRDRVLVSA
                     AVDPRYVETLRTYGRGAGYQPEVFDVVDGRGGEPEVADDVACVIVQHPNAYGLLEPAR
                     ELFGAAHA"
BASE COUNT          763 a         1709 c         1708 g          830 t
ORIGIN      
        1 cgtgaaggag cacggtcccg acggcgcgag gtatttcgac ggcgatgact acatcgtgct
       61 gtcgatcgtg aaggacttct tccgcttcgg cgtcgagccc cggcacctga cgatgtacaa
      121 gcacttcgag gaacgggagg cggacttctt cgagtcgatc gtgatgcctc agctccgtca
      181 gcggaacccc gatgcccgtc ggggtgcgac ggcctccctc gtcgagctcg ccagggcatc
      241 gcgcaaactg aagcaggccc tgctgcgcac caaccttcga cagcacctgt cgtgatccgt
      301 cggtccgcgc gcgcgtcatc gcgccggccg gttcctctcg cgccgatcgc ggtgttcgcg
      361 ctgatctcgg tgccgctcct gtcgttgggc gcggcccgcg cgcaatcgcc cacgccgcag
      421 ccgcctccac cgacgccggt catccagccc ggcgggcagg ccagcctctc gccgttcccc
      481 tcgacactgc ggacccccgc gacctcgacc gaggtccccg agatccatgc ggcgtcggcc
      541 gtgctcgccg atctcgatac cggccaggtg ctgttcgatc tcgaccgcaa cgaacggcgg
      601 cccatcgcga gcgtcacgaa gatcatgacc gcgctgctcg tcgtcgagcg cgccgacctc
      661 accgacgccg tcacggtgag cgagggcgcc gccagcggcc cggtctcggg catctcgggt
      721 ctcggtctcg tggcgggtga gcggatccgc gtggacgagc tgctgtacgc cctgctgctc
      781 cagtcggcca acgacgccgc cgtggcgctc gccgaacacg tctcgggctc ggtggacgcg
      841 ttcgtggacg acatgaacgt gcgcgccgag caactcggca tgacgcggac gatgttcacg
      901 tcaccgaacg gcctcgacga cacggggtat tcgtcggcgg ccgacctcgt ccggctcacg
      961 cgtgccgcct atcgcagccc cggcttcgcg agcgtggtgg cgacccggtt ccacacggtc
     1021 gagtccctcg acgccaaacc caggatcgtc cagaaccgca acgtgctgct gtggctgtac
     1081 cccggtgcga tcggggtcaa gacggggtac acgagtcctg cggggttctg cctcgtcgcg
     1141 gcggcgcagc gcggggacga gcgactggtc gcggtggtcc tcggcgcgcc gggcgagccg
     1201 ttctcggctg cggccgcgct gctggactac gggttcgacg cgttcgagcg ccggacgctc
     1261 ctgcgggagg gggagcgcct cgatgacgtg gtcgtcggcg gccggagcct ctcggtgagc
     1321 gccggcgaga gcgtgcgggg tctgatcccg tcggcggcga ccgcttcgcg caccgtggag
     1381 ctcgagcccc acgtggcctt cccaccggcc cggggggagg agatcggcat cgtccgggtc
     1441 tcggccgggg ggaggaccgt tggggaggcg cccctggtgg tcgtgggcgt cccgggcccg
     1501 ccgccgcccg aagcgggctc gtggtggtcc cgagccgcgg gagccctcgt cgacgcgacc
     1561 ggcggggtcc tggacgcgtt gttcggttag ccgactgcta cgatcggctc cggtgtcggg
     1621 cgtcgtcagc gtcgagttcg ggcgcgagga catccgccgg cggatcgagg agcttggccg
     1681 caccatcacc ggggactatg aggggcgcgc gcccgtcttg atctcggtcc tgaagggcgg
     1741 ctctgtgttc ctcgccgacc tgatgcgtgc cgtctcgctc ccgctctcga tcgactacat
     1801 gtccatctcc cggtacgggg gcgccaccga gtctctcgga cgcgtgcgga tcgtcaagga
     1861 tctcgatcag gacatcggtg gtcgcgatgt catcgtggta gaggacatcg tggacacagg
     1921 tctcacgctc gcgtatctga tctcggtgct ccgatcgcgc gacccggcgt cggtcgaggt
     1981 gtgtgcgctg ttggacaaga gcgttcgccg catcgtcccg ctcgagctgc ggtaccgggg
     2041 cttcgaatgt cccgacgtgt tcgtcgtcgg gtatggcctc gacttcgagg agcggttccg
     2101 taacgtcccc gacatcctcg cactccacca gatggaggcg ctccgggccg atcccgacct
     2161 gctgatcccc taccttcctg cgctcgatcc cgccccctcg acgggagggt tcgacccgcc
     2221 ggagccgtcg aggtaggact aggttgccgc acctgggggg ccggtgtacg gtcggttcgg
     2281 ttccatgatc gagatgaccg tcgccggcgt tcggatcgag ttcccgtcga actcgcccat
     2341 cgtcctgctc cgggaggccg aaggcgagcg gttcctcccg atctggatcg gccagtccga
     2401 ggcccaggcg atcgcgatcg ggcagcaggg tctggtaacg cctcgaccca tgacgcacga
     2461 cctgctcaag aacatcctgg atgagatgtc ggtgcaggtc acgagcatca cgatcaccga
     2521 gctccgggac ggcgtgttcc acgcggtgat cggcttgcag cgcaacggtt ccagcttcga
     2581 gatctcctcg cgaccctcgg acgcgatcgc gctcgccgtt cgcttggggt gcaagatctt
     2641 cgcgaacgag gacgtgctcg aagaggcctc catcctgatc cccgcggacg gcgaggaaga
     2701 ggagaaacag ctgcaggagt tccgcgagtt cctggagaac gtgacgcccg aggatttcga
     2761 gtagagcctc tacccgtaga acagctccgt cgcccagagc caatgtcgcc cgcacgtgtt
     2821 caccgagtcg acctcgatca cgccgatccc gatccgacgg atcttcgagt tcagcaagat
     2881 tgcgcgatgc accggatcat gcatgaacgc gttgtgcagc gcggagagcg tgtcggcgca
     2941 cccgaccgcg ctggcgccga cgacgtccca cggttcgtcc tggaggatcc tcgtcaggtt
     3001 cgtcggatcg tagatcgcgt tgctcgcgat catgtgacgc gtgtgtcgca gcgcgtctcg
     3061 cgagagcgaa ctgtcgatgc gcagcgtgtg cagaccgtgg ttgttgcgcg tcgcgttcac
     3121 catccggagt accttcgaac gcataccgcc gccggcacga gcggccggcg cgaacgcgat
     3181 cccgagcgca aggaccgccg acatcgtgac aaccaccgtc cgtcgcggct tgctcgtgcg
     3241 ggtccccaac cgcaacaccc ccctcgtatg cccgtcgcga aaccgcgcac gttagcgccc
     3301 agctatgcgg tcgtcatgtc cccaggtcac cctaggttca aacaccgcga ggggtgggat
     3361 ttacggccca agcggatcgg cgcaggccga agggccgacg ggagcgggtg tttgacatcg
     3421 cgggggctcg gccctaggct gccgacgccc gcgtaattac ggagttgtga tggctgagca
     3481 gggatatcgc gtccccgagg tctgcaacat cgtcgggatc acctatcgac agctcgacta
     3541 ctgggcccgg acgggcctgg tcaggccgtc ggtcaaggac gccaagggct cgggctccca
     3601 acgcctgtac tcgttccagg acctggccat gctccggctg atcaagcgga tgctcgacag
     3661 cggggtgaac ctccagcagg tccgcaaggc catgaagacc ctcaagggcc tcgaccaacc
     3721 cgccctgggg acgaccctcg tgtcggacgg ccaccgtatc tatcaggtgg agtctcccga
     3781 ggcggtcgtc gacctgctgg cgagcggaca gggggtcttc gcgatcgcgg tcgacaaggt
     3841 atggacggac ctcgagggga ctctggccaa gaacgccggg aagaagggtc gaagcgcccg
     3901 ggcggccgga ggcgcgtgac cgggggcgcg gcggccgaga ccccggccct cccgagcgac
     3961 acggtctcct tcgcgcacgc cagcgagcgc cagttcgccc agctgctgga cttctaccag
     4021 atcgaatggg actacgagcc caggtcgttc gacctcgagt gggaccatga cggcaacgtc
     4081 gtccagcgct tcacgccgga tttctacctg ccgatgtacg acctctacat cgagatcacg
     4141 acgctcaacc agcggctcgt gaccaagaag aatcgcaagg tccgccgact gcgcgagctc
     4201 tacccggaga tcaagtgcaa gatcttctac caacgcgact acctgtccct cgtgtccaag
     4261 tacgggctcg aggacgtgtc ggggtagggg tcgggggcct ctccgcgcct tccggctaga
     4321 ctcaccatcc ggcatgaggt tcgcgccgca caccgacgac gacgtcgacc agatgctcgg
     4381 ggccatcggc ctgtcgagcg tggacgagct gttcgaccgg atcccccgga cggttcgact
     4441 gaaccgtccc cttgatctgc ccgacggcgt ctccgagatg gagatccagg ccgacctcgc
     4501 ggcgctcgcc gggcggaacc gctcggcgga ggacctggtc tgcttcgccg gcggaggggc
     4561 gtacgaccat ttcgtgcccg ccgtcgtgtg ggcgctcgcc ggccggtccg agttctacac
     4621 gtcctacacg ccgtaccagc ccgagctctc acaaggcgtg ctgcaggcgc tgttcgagta
     4681 ccagtcgatg atctgcgacc tgaccgggct cgaggtctcg aacgcgtccc tgtacgacgg
     4741 cgcgaccgcg ctcgctgagg ccgtgaacct ggcgcgatcc tcgccgggac gcgaccgggt
     4801 gctcgtgagc gccgccgtcg acccacggta cgtcgagacc ctgcggacct acgggagggg
     4861 ggcggggtac cagcccgagg tcttcgacgt cgtcgacggg cgcggcggcg agccggaggt
     4921 ggccgacgac gtcgcgtgcg tgatcgtcca gcacccgaac gcgtacggcc tgctcgagcc
     4981 ggcgcgcgag ctgttcgggg cggcgcacgc
//
LOCUS       DATBRK010000004         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127 k141_229,
            whole genome shotgun sequence.
ACCESSION   DATBRK010000004 DATBRK010000000
VERSION     DATBRK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VIH70_00090"
     CDS             complement(<1..876)
                     /locus_tag="VIH70_00090"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEY8114795.1"
                     /translation="MNDPRDVVAAKRDGRTLEPDELRSFVRAYARGELPDYLAAAFLM
                     AAFINGLDASETLALTQAMVDSGDTLPLGGISRPKVDKHSTGGVSDGVTLVFAPIAAS
                     LGLAVAKLSGRGLGHTGGTLDKLESIPGLRTDLKPHAFERQIEEVGSAVAAQTTELVP
                     ADGALYALRDATATVPSVPLIAASVMSKKLAIDTDLVLLDVKAGSGAFMKTPDDAAEL
                     AEACTALAKDWGRPTRAAVTDMSQPLGEAIGNALDVVEAVRLLRGELHGRLRDVAVMF
                     AGEAVSRLSDVPMGEG"
     gene            complement(873..>1541)
                     /locus_tag="VIH70_00095"
     CDS             complement(873..>1541)
                     /locus_tag="VIH70_00095"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEY8114796.1"
                     /translation="PLPTLYEWCRVARRLLTGRHNVGRVIARPFTGEPGAFVRRPERR
                     DFSVPPPGPTLLDRCVDDGVAVYGVGKIQDIFAQQGLTEARYSDSNDHGVDLTIDFLR
                     RPGPALVFGNLVDFDSKYGHRNDPEGYAHAIEALDARIPELVGALGGGVLLLTGDHGC
                     DPTTPSTDHSRERTPLLAAGLEGGPYDVGDRDTYADLGATAAELLGVPWGLPGSSFAG
                     EIGR"
BASE COUNT          219 a          563 c          541 g          218 t
ORIGIN      
        1 cccctccccc atcgggacgt ccgagagccg ggacaccgcc tcgccggcga acatcaccgc
       61 cacgtcacgc aggcgtccgt gcagctctcc gcggagcaac cggacggcct cgaccacatc
      121 gagggcgttc ccgatcgcct ctccgagcgg ctgcgacata tcggtcaccg cggcccgcgt
      181 cggacgcccc cagtccttcg cgagcgccgt gcacgcctcg gcgagctcgg ccgcgtcgtc
      241 gggcgtcttc atgaacgccc cgctccccgc cttcacgtcg aggagcacga ggtcggtgtc
      301 gatcgcgagc ttcttcgaca tcacgctcgc cgcgatcagc ggcaccgagg gaaccgtcgc
      361 ggtcgcgtcc cggagcgcgt agagcgcgcc atcggccggc acgagttcgg tcgtctgcgc
      421 cgcgaccgcc gacccgacct cctcgatctg ccgttcgaag gcgtgtggct tcaggtcggt
      481 gcgcaggcct gggatcgact cgagcttgtc gagggttccc cccgtgtggc cgagcccgcg
      541 cccggagagc ttggccaccg cgagcccgag cgacgccgcg atcggcgcga acacgagcgt
      601 cacgccgtcg gacacgccgc ccgtcgagtg cttgtcgacc ttcggtcgcg agatgccgcc
      661 cagcggtagc gtgtcgcccg agtcgaccat cgcttgcgtg agggcgagcg tctccgaggc
      721 gtcgaggccg ttgatgaacg ccgccatcag gaaggccgcg gccaggtagt ccgggagctc
      781 gccgcgcgcg taggcccgga cgaacgaccg gagctcgtcg ggctcgagcg tccggccgtc
      841 gcgtttggcg gcaaccacgt cccgagggtc gttcaccggc cgatctctcc ggcgaagctc
      901 gagcccggca ggccccacgg caccccgagg agctccgcgg ccgttgcgcc gagatccgcg
      961 tacgtgtcgc ggtcgcccac gtcgtacggc ccgccctcga gcccggcggc gagcagcggg
     1021 gtgcgttcgc gcgagtggtc ggtcgacggc gtggtgggat cgcacccatg gtcgccggtc
     1081 agcaacaaca cgccgccacc gagcgcgccg acgagctcgg ggatcctcgc gtcgagagcc
     1141 tcgatcgcgt gcgcatagcc ctcggggtcg ttgcgatgcc cgtacttgct gtcgaagtcg
     1201 acgaggttgc cgaacacgag tgccgggccg ggacggcgca ggaagtcgat cgtgaggtcg
     1261 accccatggt cgttcgaatc cgagtaccga gcttcggtca gtccctgctg cgcgaagatg
     1321 tcctggatct tgccgacccc gtacacggcg acgccgtcgt cgacgcagcg gtcgagcaga
     1381 gtcggaccgg gcggcgggac cgagaagtcg cgtcgctccg gccggcggac gaacgcaccg
     1441 ggctcccccg tgaacgggcg cgcgatcacg cgacccacgt tgtgcctgcc ggtcagcaga
     1501 cgtctggcga cgcggcacca ctcgtacagc gtggggagcg g
//
LOCUS       DATBRK010000005         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_578147, whole genome shotgun sequence.
ACCESSION   DATBRK010000005 DATBRK010000000
VERSION     DATBRK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578147"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /gene="dxr"
                     /locus_tag="VIH70_00100"
     CDS             complement(<1..1154)
                     /gene="dxr"
                     /locus_tag="VIH70_00100"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY8114797.1"
                     /translation="MRSLVILGSTGSVGTQALDVVRHNPDRFKVVGLSAGTNQELLVG
                     QIREFLPPHVAIANDEAAVDLEEKLGGIKGVEVHVGPEAAESLARDSEADMVLNALVG
                     SAGLAPTLATLQAGKLLALANKESLVVGGDLVVDLIKGEPERLIPVDSEHAALAQCLR
                     GERREDLKRVILTGSGGPFRGWTHAELAKASVKDALAHPVWQMGPKITIDSATLMNKG
                     LEVIEAHYLFGLEYSQIHAVIHPEGLVHALAEFRDGSMTAQLAVPDMRLPIQLALAWP
                     ERLPTGVGPVPLADSRLSFEPVDREAFPAIDLSYRVGELALTFPAVMSAANEVAVMAF
                     LEGKIPLTRIVEVVTAVVDEHEAPSSVVSVVNLERADTWARRRAAEIIEAR"
     gene            complement(1222..2778)
                     /locus_tag="VIH70_00105"
     CDS             complement(1222..2778)
                     /locus_tag="VIH70_00105"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY8114798.1"
                     /translation="MPEDEERNEGEDLFEDLDKFFAPIQDVDWPESSEGERSEAAGRE
                     RREEPSRAEPEQPEDGSPEPEDEEAAAAEPDAGQPLGGDESFAAQEPSDADVEDQGGV
                     GSFMFEAEAEGYEVDEGAEAGSSGYVDLPGDEPGVAELEETETVVVPEVAPEKPPPEK
                     LEDAPDLEAVEAAADHFAESVRDEVQLEPERAPQPTEEVAIAQLLGEEGASDEPETSR
                     TVRVGAEGFGGPSWQEPTAIEVGAESDRAGPGRDVPAAFITGIVLAGLAVGAILIGAG
                     AFAIYAGIVVLFALGEFYLAMQKRHYQPATALGLVSGALVLGAGYYRGENAMLAIMAL
                     SLFATFLWYMTVPPQHRRNVVSNIAITILGVAYIPLLAAYALAVLKLPDGEGLTLSII
                     GLTFAYDTAAFGVGYFWGSRPLAPTISPKKSWEGAIGATLVVIAVAVGVVGSSVSLLD
                     TVGRSVGLAVVVALFAPLGDLAESLLKRDLEIKDMGGILPGHGGVMDRIDSVLFVAPA
                     AFIYLRLILL"
BASE COUNT          423 a         1032 c          980 g          423 t
ORIGIN      
        1 cgagcctcga tgatctcggc ggcacgccgg cgagcccagg tatcggcccg ctcgaggttc
       61 accaccgaga ccacgctcga gggcgcctcg tgctcgtcga cgacggccgt gacgacctcg
      121 acgatgcgcg tgagggggat cttgccctcg aggaaggcca tgaccgcgac ctcgttggcc
      181 gcgctcatga ccgcggggaa ggtcagagcg agctcgccga cgcgatagga caggtcgatc
      241 gccgggaacg cctcccgatc gacgggctcg aacgacaggc gactgtcggc gaggggaacg
      301 gggccgacgc cggtcgggag ccgctccggc cacgcgagcg ccagctggat gggcagccgc
      361 atgtccggga ccgcgagctg agcggtcatc gacccgtccc ggaactccgc gagcgcgtgg
      421 acgaggccct ccgggtggat gacggcgtgg atctgcgagt actccagccc gaacaggtag
      481 tgcgcctcga tcacctcgag gcccttgttc atcagcgtcg ccgagtcgat cgtgatcttc
      541 gggcccatct gccagacggg gtgtgcgagc gcatccttga ccgaggcctt ggcgagctcc
      601 gcgtgggtcc agccccggaa ggggccgccc gacccggtca ggatcacgcg cttcaggtct
      661 tcgcggcgct ccccgcgcag gcattgggcg agcgcggcgt gttccgagtc gaccgggatg
      721 agccgctccg gctcgccctt gatcaggtcg acgacgaggt cgccaccgac gaccaggctc
      781 tccttgttgg cgagggcgag cagcttgccc gcctgcaggg tcgcgagcgt gggcgccagg
      841 ccggccgacc cgaccagcgc gttcaggacc atgtcggcct cggagtctcg cgccaggctc
      901 tcggcggcct cgggccccac gtggacctcg acccccttga tcccgccgag cttctcctcg
      961 aggtcgacgg ccgcctcgtc gttcgcgatc gcgacgtgcg gcgggaggaa ctcccggatc
     1021 tgcccgacca ggagctcctg gttggtgccg gcggacaggc caacgacctt gaaccggtcg
     1081 gggttgtgcc gcacgacgtc gagcgcctgc gtccccaccg atcccgtcga gccgaggatg
     1141 acgagcgagc gcatcgcctc aacgataccc cgaggcgccc acaaaccacg gaagggcgct
     1201 gaccccgggg tgtgggaggc ctcagagcag gatcaggcgg aggtagatga aggcggcggg
     1261 ggcgacgaac aacaccgagt cgatccggtc catcacgccg ccgtgaccgg gcaggatccc
     1321 gcccatgtcc ttgatctcga ggtcgcgctt gagcagcgat tcggccaggt cgccgagcgg
     1381 cgcgaacagc gcgaccacga ccgccaggcc gaccgaacgc ccgaccgtgt cgagcagcga
     1441 gaccgacgag cccacgaccc cgacggcgac cgcgatcacc acgagcgtgg ccccgatcgc
     1501 gccctcccac gacttcttcg ggctgatggt gggcgcgagg ggacggctcc cccagaagta
     1561 gccgaccccg aaggccgccg tgtcgtaggc gaacgtgagc ccgatgatcg agagcgtcag
     1621 cccctcgccg tcggggagct tgaggacggc gagcgcgtac gccgcgagca acgggatgta
     1681 cgccacgccg aggatcgtga tcgcgatgtt cgacacgacg ttgcgccggt gttgcggcgg
     1741 aacggtcatg taccagagga aggtcgcgaa cagggacagt gccatgatcg cgagcatcgc
     1801 gttctcgccg cgatagtagc cggctcccag aacgagcgcg ccggagacga gcccgagtgc
     1861 cgtcgccggc tggtagtgcc gcttctgcat cgcgaggtag aactccccca gagcgaacag
     1921 cacgacgatg ccggcgtaga tcgcgaacgc tcccgcgccg atcaggatcg cgccgacggc
     1981 caagcccgcg agcacgatgc cggtgatgaa cgccgccggg acgtcgcggc cgggtccggc
     2041 gcggtcggat tcggcgccca cctcgatcgc tgtcggctcc tgccagctcg gaccgccgaa
     2101 cccctcggcc ccgacccgga ccgtgcgaga ggtctcgggc tcgtccgacg cgccctcctc
     2161 tccgaggagc tgggcgatcg cgacctcctc ggtgggctgt ggcgcccgct ccggctcgag
     2221 ctgcacctcg tcccggacgg actcggcgaa gtggtcggcc gcggcctcga cggcctcgag
     2281 gtcgggtgcg tcctccagtt tctctggtgg tggcttctcc ggcgcgacct ccgggaccac
     2341 caccgtctcg gtctcctcga gctcggctac gcccggctcg tcgccgggga ggtcgacgta
     2401 cccactcgag ccggcctcgg ctccctcgtc gacctcgtag ccctccgcct cggcctcgaa
     2461 catgaacgag ccgacccctc cctgatcctc gacgtcggcg tcagacggct cctgggccgc
     2521 gaacgactcg tcgccgccaa ggggttggcc cgcgtcgggc tcggcagccg ccgcctcctc
     2581 gtcctccggt tcgggggaac cgtcctccgg ctgctcgggt tcggcccgcg acggttcctc
     2641 gcggcgctcc ctgcccgctg cctccgacct ctccccctcg gacgactccg gccagtcgac
     2701 gtcctggatc ggcgcgaaga acttgtcgag atcctcgaag aggtcttcgc cctcgttccg
     2761 ttcctcgtcc tccggcacgc tcagatctcc ttgagctcgg catccttgtg ggccacgacc
     2821 tcgtcgatgt ccgagatgtg cttgtcggtg agcttctg
//
LOCUS       DATBRK010000006         4108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_289255, whole genome shotgun sequence.
ACCESSION   DATBRK010000006 DATBRK010000000
VERSION     DATBRK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289255"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VIH70_00110"
     CDS             <1..370
                     /locus_tag="VIH70_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114799.1"
                     /translation="GPSPTGWFDPRGRAIGSWAFAVNRITGLGLVFYLYLHLGVLSLL
                     LAGESAWNEFLEIATTTVFLGLDVLLLFGILFHGLNGVRVALVGTGVVPNRQMALFWA
                     GTVIGTIVLVSGAVHIVGSG"
     gene            372..773
                     /locus_tag="VIH70_00115"
     CDS             372..773
                     /locus_tag="VIH70_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114800.1"
                     /translation="MTEVPPRTDPTRDRQVPSGRSSRAWWWTALTGAAILLLLTVHMV
                     AHHFVVEDVGGLRSYEQVLDYIANPVIFALEVFFLIVVTIHALLGLRGVLFDLGIGVR
                     ARRRTDLLLWLLGLVTVGYGFFLLGTLASRA"
     gene            787..3297
                     /gene="ptsP"
                     /locus_tag="VIH70_00120"
     CDS             787..3297
                     /gene="ptsP"
                     /locus_tag="VIH70_00120"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY8114801.1"
                     /translation="MVGIVLVSHSHSVAEGAAELARQMGGQDVRIETAGGLDDPERSM
                     GTDAVLVMQAVERAWSDDGVLVLMDLGSAVLSAEMALDLLPVERRDRVLLTDAPLVEG
                     AVSAAVSARIGSNLEEVAAEARGGLLAKTTHLGMDGPSQAANAEAAAEAPGASVRLAV
                     RNAHGLHARPAARLVQTASAFDASVRVSNLTTGEGPASARSLNAVATLGVVAGNEIEV
                     SASGRQAREAVDAIRALAERGFDEGVEPAEIEEGPRRTSETLEERILGLPASPGIAVG
                     EIRRFHVPAIEVPERQASDAADEDRRLDAAIATARDDIEHQRVSVARRAGPYQAAIFE
                     AHLLFLDDQELIAPARRAIVTGATAEKAWRDSIEDMARAWERLDDEYQRARAADLRSV
                     GAQVLARLLGIDPPRPKLEAAGVVVAPELSPADAAALDPAVALGIVTAFGGPTSHAAV
                     LARSIGIPAVVGAGEDVLDLAEGTTLAIDGELGVVRVDPSGRELEAFEARARERRVAE
                     EAARATARSPAATADGVLIKVDANVGSPTEIATAVDAGCDGVGLFRSEFLFLGRDAMP
                     TEDEQEASYRASAEALEGRRLIVRTLDAGADKPLPFIDQPAEDNPFLGVRGLRLSLAR
                     PELFDSQLRAILRVAADHPVGVLFPMVTSVEELRAGRAAVERARDGLAAIGRKVPERL
                     TLGAMIEVPSAALLADRLAGEVDFFSIGTNDLTMYTLAADRGNERVAQISDALDPAVL
                     ELIHRTTAAGNARGIWTAVCGELAGDPSAATLLIGLGVRELSMSASSIPHVKAAVRAT
                     DLRRAEAAAREALSLSSAGEVRGLLHGLGHGAGATMPQ"
     gene            3824..3896
                     /locus_tag="VIH70_00125"
     tRNA            3824..3896
                     /locus_tag="VIH70_00125"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3857..3859,aa:Ala,seq:tgc)
BASE COUNT          592 a         1399 c         1479 g          638 t
ORIGIN      
        1 aggcccgtcg ccgaccgggt ggttcgatcc tcgtggccgc gcgatcgggt cgtgggcgtt
       61 cgcggtgaac cgcatcaccg gtctcggcct ggtcttctac ctctacctgc acctcggcgt
      121 cctgtcgctg ctgctcgccg gagagtcggc gtggaacgag ttcctcgaga tcgcgacgac
      181 cacggtcttc ctggggctcg acgtgttgtt gctgttcggg atcctgttcc acgggctcaa
      241 cggcgtgcgg gtcgccctgg tcggcaccgg ggtcgtcccc aaccggcaga tggcgctgtt
      301 ctgggcgggc acggtgatcg gcacgatcgt gctcgtctcc ggcgccgtcc acatcgtcgg
      361 gagcggatga gatgaccgag gtgccgcccc gaacggatcc gacacgcgac cgccaggtcc
      421 cgagcggccg gtcatcacgc gcatggtggt ggaccgcgct caccggcgcc gcgatcctcc
      481 tgctgctgac cgtccacatg gtcgcccacc acttcgtcgt cgaagacgtc ggcgggctac
      541 gttcgtacga acaggtgctg gactacatcg cgaacccggt catcttcgcg ctggaagtct
      601 tcttcctgat cgtggtcacg atccacgcgt tgcttggact acgcggcgtc ctgttcgatc
      661 tcgggatcgg ggtccgcgcg cgccggcgga ccgatcttct gctgtggctc ctcggcctcg
      721 tcacggtcgg ctacggcttc ttcctccttg gcaccctcgc ctcgagggcg tgaggcttcc
      781 ctcaccgtgg tgggcatcgt cctcgtctcg cacagccact cggtggccga aggcgcagcc
      841 gagctcgccc gccagatggg tggacaggac gtgcggatcg aaacggcggg ggggctggac
      901 gaccccgaac gctcgatggg cactgacgcg gtgctcgtga tgcaggccgt cgagcgcgcg
      961 tggtccgacg acggtgtgct ggtcctgatg gacctgggga gcgccgtcct gtcggccgag
     1021 atggcgctcg acctcctgcc ggtcgaacgc cgcgacaggg tgctgctgac cgacgccccc
     1081 ctcgtggagg gagccgtgtc ggcggcggta tccgctcgga tcggctcgaa cctcgaggag
     1141 gtcgccgccg aggctcgcgg agggctgctg gccaagacca cgcacctggg gatggatggg
     1201 ccgtcgcagg ccgcgaacgc cgaagcggcc gccgaggccc ccggtgcttc cgtccggctg
     1261 gctgtacgga acgcccacgg tctgcacgcg cggccggcgg cgcgcctggt ccagaccgcc
     1321 tccgcgttcg acgccagcgt gcgcgtctcc aacctcacga ccggagaagg tccggccagc
     1381 gcccggagcc tcaacgcggt ggcaacgctc ggcgtggtgg ccggcaacga gatcgaggtc
     1441 tcggcatcgg gtcggcaagc gcgcgaggcc gtcgatgcca tccgagcgct cgccgagcgt
     1501 gggttcgacg aaggagtgga accggccgag atcgaagaag gcccgcgccg gacatccgag
     1561 acgctcgaag agaggatcct cggtctgccc gcgtcacccg gcatcgccgt gggcgagatc
     1621 cgacggttcc acgtgcccgc gatcgaggtt cccgaacgac aggcgagcga cgcggccgat
     1681 gaggaccggc gcctggacgc cgcgatcgcc acggcccgcg acgacatcga gcaccagcgg
     1741 gtctcggtgg cccgtcgcgc cggcccgtac caggcggcga tcttcgaggc gcacctgctg
     1801 ttcctcgatg accaggagct gatcgcgccg gcacgacgcg cgatcgtgac gggcgcgacg
     1861 gcagagaagg catggagaga ctcgatcgag gacatggcgc gagcatggga acggctcgac
     1921 gacgagtatc agcgcgcccg tgccgccgat ctccgaagcg tcggcgcgca ggtgctggct
     1981 cggctcctgg gcatcgatcc gccgagaccg aagctggagg ctgccggcgt ggtcgtggcc
     2041 cccgagctgt cgccggccga cgccgcggcg ctcgacccgg cggtcgcgct cggcatcgtg
     2101 accgcgttcg gcggcccgac gtcccacgcc gcggtgctcg cccgttcgat cggcatcccc
     2161 gcggtggtcg gggcgggcga ggacgtcctc gacctcgccg agggcacgac gctagcgatc
     2221 gacggcgagc tcggcgtcgt tcgcgtcgac ccgtccggcc gcgagctcga ggcgttcgag
     2281 gcccgtgcgc gcgagcgccg ggtggccgag gaggcggcgc gcgcgaccgc acgatctccc
     2341 gccgccaccg ccgacggcgt cctgatcaag gtggatgcca acgtggggtc gccgacggag
     2401 atcgcgaccg cggtcgacgc cggctgcgac ggggtgggcc tgttccggtc ggagttcctg
     2461 ttcctcgggc gcgatgcgat gccaacggag gacgagcagg aggcctcgta ccgagcctcg
     2521 gccgaagctc tcgaggggcg gcggctcatc gttcgaacgc tggatgcggg cgccgacaaa
     2581 ccgctcccct tcatcgatca gccggccgag gacaacccgt tcctcggtgt cagggggttg
     2641 cggttgagcc tcgccagacc cgagttgttc gactcgcaac tgcgggcgat cctgcgcgtc
     2701 gcggccgatc atccggtggg cgtgctgttc ccgatggtca cgagcgtgga ggagctccga
     2761 gccggccggg cggccgtcga gcgcgcccgt gacggcctgg ccgccatcgg gcggaaggtt
     2821 cccgagcgcc tgacgctggg cgccatgatc gaggtgccct cggcggcgct cctggccgac
     2881 cgtcttgcgg gcgaggtcga tttcttctcg atcggcacca acgacctcac gatgtacacg
     2941 ctggccgccg atcgcgggaa cgagcgggtc gcacagatct ccgatgcgct cgatccggcc
     3001 gtcctggagc tgatccaccg cacgaccgcc gccgggaacg cgcgtggcat ctggaccgcc
     3061 gtctgcggcg agctcgcggg cgacccgagt gcggcgaccc tgctgatcgg cctcggcgtc
     3121 cgcgagctct cgatgagcgc ctcctcgatc ccccacgtca aggccgcggt gcgcgcgacc
     3181 gatctccggc gggccgaggc tgccgcgcga gaggccctca gcctctcctc ggcgggcgag
     3241 gtccgggggc tgctgcacgg actgggccat ggagccggtg ccacgatgcc gcagtagcgg
     3301 tgacaggagg ccagcagatc gctctgatca ctcaggagat cggcgtgttg tcggcaagct
     3361 tcggttcgga gctgcgacga tcacacggat cgccgagctc tcggccgcag agctcctggc
     3421 tgaggtcggt gacatcggcc gctacgcacc aaggcccagt tcgcgttggc cacggcacca
     3481 caccgccgac cgccacttcg ggccgtatcg atcgcgcttg aaccgtacgg ctagcttcgg
     3541 tacggcgcag cgacccgatg ggttcgcgtt atcgtgcgta agccgacgga gtggttcgcg
     3601 tgcctcgtga tgtcggaacg cacaagggcc aacgtcacga gaacgccgcc gaacgcaggg
     3661 gtaggtgcct cactgtccgg ggaggcgaac accggtcgac gacggcggcc gagcgccgtg
     3721 tcccgtcgaa ggggagacgt tccatggacc cgggcgctgt agagttattg ggctgcaaga
     3781 gcggtgtgca agacgatgat tcagtgtttc cgcaggtaaa cacggggacg tagctcagct
     3841 ggtagagcac ctgctttgca agcagggggt cggcggttcg agtccgctcg tctccacgaa
     3901 tagattgtgc gaagcgggcg gtccggttgc gggccgcccg cttcgcgaat caccgtcagg
     3961 atctggaaaa cggccgaacg agcagatgga gctacccggc gagcacacgg ccctgcatgg
     4021 cttcgagctt gcccgcgtcc cacagcggtg cggcaccctg gaggcgaaca gtctctgcga
     4081 gcacacggcc ccgcatggct tcgagctt
//
LOCUS       DATBRK010000007         4058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1300428, whole genome shotgun sequence.
ACCESSION   DATBRK010000007 DATBRK010000000
VERSION     DATBRK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300428"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VIH70_00130"
     CDS             complement(<1..276)
                     /locus_tag="VIH70_00130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8114802.1"
                     /translation="MSSEWDAETYDAIADPQFRWGAAVVDRLELDGDETVLDAGCGSG
                     RVTELLARRLPRGRVVALDGSSAMLGTARRRLAPFGDRITFVHAELLD"
     gene            complement(287..1096)
                     /locus_tag="VIH70_00135"
     CDS             complement(287..1096)
                     /locus_tag="VIH70_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY8114803.1"
                     /translation="MSTDATQPVLRGLVLAAALATTLAACGSSAGDGPSAAPRQESST
                     ATPPSPTPTAEPATDPAGMEPVERSGFHGKIDPLPTTLAEQMRGTTWHPGCPVPLEGL
                     SLLHFNYWGFGGNVLRGPMVVNSTVAEDVLWVFHQLYDARFPIKRVSLATPFHPKAFA
                     QHRRIDSNRSVTASFNCRPVVSATGPGDDFSQHAYGLAVDINPLQNPYVTTDGFVRNR
                     AAEPYTVRSRHLEGMIHEGDVVVRTFAAIGWEWGGDWSGDKDYMHFSLLGR"
     gene            complement(1160..2614)
                     /locus_tag="VIH70_00140"
     CDS             complement(1160..2614)
                     /locus_tag="VIH70_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8114804.1"
                     /translation="MKSTRRRAREALRSDDFRRLFAIRLITQSGDGLFQAALVSSIVF
                     NPKEQNTIMGFAIATLVVSLPFSILGPFTGVFIDRWSRRKILVVAPWLRAAAVWLVLF
                     DPTAAPVLFFGGALWVLSVNRFYLATAQAVVPRMVPTEDLLMANSMATVGGTVALLIG
                     VFVGGWIADLVGHVPVIAIAGAEWLAASMVATRIRSPLVPHHVPEAPVRDELRRVARE
                     FADGISRLAGTPRALAPITSITLDQVGQGVVLVLSLYVFRHRFGEGVGSFSNLIGAGG
                     VGVLLGILTVGKLEERLTKERIVALAFLAGGVVLIGVATYVTGWSVLIASFAIGLTFA
                     WKKIPVDTLVQEAIPDGYRGRVFAVYDVAYNLSRVAAAFLAIPLLNAFGEAWAVAIVG
                     VVFILWSPVLPRWIGRIPELSIRFYEGGRAEEDPRAVVWGGVEETVDVERRSLEETDG
                     VRRTRYRLRMLDGTTIEVSKVEPDGAWTLERELE"
     gene            complement(2651..3514)
                     /locus_tag="VIH70_00145"
     CDS             complement(2651..3514)
                     /locus_tag="VIH70_00145"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8114805.1"
                     /translation="MTAVAVLGLGAMGRAIGARLLGDGHDVRVWNRTPGKDDDLVASG
                     ARRAATAADAVRDVDVVITMVTDPPALEAVLFGPDGAAPAIPQSATLIDMSTVGPSEI
                     ASVVDRLEPVAVLDAPVLGSVPSVESGTLVILVGGERAVFERRTELLSLLGTPIYVGP
                     SGSGAWLKLVNNAAGTTALVAVGELLGLTDRAEIDLDVALDSLAAGPLASLIERWRPR
                     LTGEDHASYFRLALARKDLALAFEEAERAGTELTVAEAAAARCDEAIGDGLGDEDFGA
                     IVPFLRQKEER"
     gene            complement(3511..3912)
                     /locus_tag="VIH70_00150"
     CDS             complement(3511..3912)
                     /locus_tag="VIH70_00150"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8114806.1"
                     /translation="MRATRSNPETVAAPMGTYSHAVRVEASDAVWIYVSGQIANDPEG
                     GLVGPGDLRAQTRQVFENLRAILEANGATFADVVKIGTYLTTLEDLDAMREVRKEYLT
                     AEPPTSTAVQVVALLVPDAMIEVDLVAVVPS"
BASE COUNT          623 a         1458 c         1426 g          551 t
ORIGIN      
        1 gtccagcagc tcggcgtgca cgaacgtgat ccggtcgccg aacggcgcca gccgtcgacg
       61 tgccgtcccc agcatcgcgc tcgagccgtc caacgccacg acccggccgc gggggagccg
      121 cctggccagg agctcggtca cgcgccccga accgcacccg gcgtccagga cggtctcgtc
      181 gccgtcgagc tcgagccggt cgaccacggc cgcaccccac cggaactgcg ggtcggcgat
      241 cgcgtcgtag gtctcggcgt cccactcgct cgacacgctc cggacgctac cgccccagaa
      301 gcgagaagtg catgtagtcc ttgtcaccgc tccagtctcc gccccattcc cagccgatcg
      361 cggcgaacgt gcgcaccacg acgtcgccct cgtggatcat gccctcgagg tggcggctgc
      421 ggacggtgta cggctcggcg gcgcggttgc ggacgaagcc gtcggtagtg acgtaggggt
      481 tctggagcgg gttgatgtcg accgccagcc cgtaggcatg ctgcgagaag tcgtcgccgg
      541 gaccggtcgc gctgaccacc ggcctgcaat tgaaggacgc ggtcacgctt cggttgctgt
      601 cgattcgccg atgctgcgcg aacgccttgg ggtggaacgg ggtcgcgagc gagacgcgct
      661 tgatcgggaa tcgcgcgtcg tagagctggt ggaacaccca caggacgtcc tcggccaccg
      721 tgctgttcac gaccatgggg ccgcggagca cgttgccgcc gaacccccag tagttgaagt
      781 gcaggaggct gaggccctcc agggggacgg ggcatcccgg gtgccacgtc gtgccgcgca
      841 tctgctccgc gagggtcgtg gggagcgggt cgatcttgcc gtggaacccc gagcgttcga
      901 cgggctccat gccggctggg tcggtcgccg gctcggccgt gggcgtgggc gacggaggcg
      961 tggccgtcga actctcctgc ctgggcgccg ccgagggacc gtcgcccgcg gatgagccgc
     1021 acgcggcgag ggtcgtcgcg agggccgcgg cgaggacgag cccccgcagg accggttggg
     1081 tggcgtcggt ggacacgaga cgatcgttct accaccgggg tccggggttc ggcggcctcc
     1141 ggcgcgaacg accctcggat cactcgagct cgcgctcgag cgtccaggcg ccgtcgggtt
     1201 cgaccttcga gacctcgatc gtcgtcccgt ccagcatccg caggcgataa cgcgtccgcc
     1261 gcacgccgtc ggtctcctcc agcgaccggc gctccacgtc gacggtctcc tcgaccccgc
     1321 cccagacgac cgcccggggg tcctcctcgg cgcgccctcc ttcgtagaac cggatcgaca
     1381 gctcggggat ccgaccgatc cagcgaggca ggacggggct ccacaggatg aacaccacgc
     1441 cgacgatcgc gaccgcccac gcctcgccga acgcgttcag cagcgggatc gccaggaatg
     1501 cggcggccac gcgcgagagg ttgtacgcga cgtcgtacac ggcgaacacg cggcctcggt
     1561 agccgtcggg gatcgcctcc tggaccaagg tgtccaccgg gatcttcttc cacgcgaacg
     1621 tgagcccgat ggcgaagctc gcgatcagca cgctccagcc cgtgacgtac gtggcgaccc
     1681 cgatcagcac gacaccgccg gcgaggaagg cgagcgcgac gatccgttcc ttggtgagtc
     1741 gctcctcgag cttgccgacc gtgaggatgc cgaggagcac gccgaccccg ccggcaccga
     1801 tcaggttcga gaacgagccg acaccctccc cgaaccgatg gcggaagacg tacagcgaca
     1861 gcaccaacac gacgccttgt ccgacctggt ccagcgtgat cgacgtgatc ggcgcgagcg
     1921 cccgcggcgt gcccgcgagc cggctgatcc cgtcggcgaa ctcgcgcgcc acgcgccgga
     1981 gctcgtcccg caccggggcc tcgggcacgt ggtgggggac gagcgggctc cggatcctgg
     2041 tggcgaccat cgaggcggcc agccactcgg cgccggcgat cgcgatcacc ggcacgtgcc
     2101 cgacgagatc ggcgatccat ccaccgacga agaccccaat gagcagcgcg accgtgccgc
     2161 cgacggtcgc catcgagttg gccatcagca ggtcctcggt cgggaccatc cgcgggacca
     2221 ccgcctgcgc cgtcgcgagg tagaagcggt tcaccgacag cacccacagt gccccgccga
     2281 agaacagcac cggcgcggcc gtcggatcga acagcaccag ccacacggcc gcggctcgca
     2341 gccacggcgc gaccacgagg atcttcctgc gcgaccagcg gtcgatgaac accccggtga
     2401 acgggccgag gatcgagaag ggcagggaca ccaccagcgt cgcgatcgcg aaacccatga
     2461 tcgtgttctg ctccttgggg ttgaacacga tcgacgacac gagcgccgcc tggaacagcc
     2521 cgtcgccgga ctgcgtgatc agccggatcg cgaacagccg ccggaagtcg tcgctccgca
     2581 gggcctcgcg ggcgcgccgc cgggtcgact tcacgccgag gatggtaggc gcgcggcctc
     2641 ggggccggcg ctacctctcc tccttctggc gcaggaacgg gacgatcgcg ccgaagtcct
     2701 cgtcgcccag gccgtccccg atcgcctcgt cgcatcgggc ggccgccgcc tcggccaccg
     2761 tgagctcggt gcccgcgcgc tccgcctcct cgaacgcgag cgcgaggtcc ttgcgcgcca
     2821 aggccagacg gaagtacgag gcgtggtcct cgcccgtcag gcgtggacgc cagcgctcga
     2881 tcagcgaggc gagcgggccc gccgccaggc tgtcgagggc cacgtcgagg tcgatctcgg
     2941 cacgatcggt cagcccgagg agctcgccga cggctacgag ggccgtggtg ccggccgcgt
     3001 tgttgaccag cttgagccac gcccccgagc cggacggccc gacgtagatc ggtgtgccga
     3061 ggagcgacag gagctcggtc cggcgctcga acacggcgcg ttctcccccg acgaggatca
     3121 cgagcgtgcc gctctcgacc gaggggacgc tgccgagcac gggggcgtcg agcaccgcga
     3181 cgggctcgag ccgatcgacg accgaggcga tctcgctcgg ccccacggtc gacatatcga
     3241 tgagcgtcgc gctctggggg atcgccggtg ccgcgccgtc gggtccgaac aggaccgcct
     3301 cgagcgccgg cgggtcggtg accatcgtga tgaccacgtc gacatctcga acggcgtcgg
     3361 ccgccgtcgc ggcccggcga gcgccggagg cgacgaggtc gtcgtccttg ccgggcgtcc
     3421 ggttccagac gcggacgtcg tgaccgtcgc cgagcagccg agccccgatc gcgcgcccca
     3481 tcgcgccgag cccgaggacc gcgaccgcgg tcatgacggc accaccgcga cgaggtcgac
     3541 ctcgatcatc gcgtccggga cgaggagagc gacgacctgc acggcggtgc tcgtgggtgg
     3601 ttcggcggtg aggtactcct tgcggacctc acgcatcgcg tcgaggtcct cgagggtggt
     3661 gaggtacgtt ccgatcttga ccacgtcggc gaacgtcgcc ccgttcgcct ccaggatcgc
     3721 gcgaagattc tcgaacacct gccgggtctg ggcgcggagg tcgcccggcc cgacgagccc
     3781 gccctccggg tcgttggcga tctgcccgga gacgtagatc cagaccgcat ccgacgcctc
     3841 gacgcgaacg gcgtgggagt acgtccccat gggcgcagcg acggtctccg ggttcgaacg
     3901 ggttgctcgc atgctcccct cctccgtgct cgtgctcgct ggagagcgta cgaggtggtc
     3961 gcggagggct aggcctccgc gggctcgatg tcgaagaacg ccgtccagtc cgcggccttc
     4021 gaggcgccgt tgcccaggat gaactgctcg accatgtc
//
LOCUS       DATBRK010000008         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_145420, whole genome shotgun sequence.
ACCESSION   DATBRK010000008 DATBRK010000000
VERSION     DATBRK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145420"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VIH70_00155"
     CDS             <1..455
                     /locus_tag="VIH70_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEY8114807.1"
                     /translation="LMFCVSKGLGAPIGSLVCGSAEQMREGRRIKILFGGAWRQAGVV
                     AAAGLVALQEGPQRLHEDHERARRLGEALAEIVPGSLDPSDVETNMVFADTEASMAMP
                     PLQVANRLEEHGVGASVVSGKVRMVTHVDIEDQDIETAIAAWQAAAAR"
     gene            467..1090
                     /locus_tag="VIH70_00160"
     CDS             467..1090
                     /locus_tag="VIH70_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEY8114808.1"
                     /translation="MKLFGPRYPEEIAKRIPPGQRLVKTWPVLHFGPIPDFDESTWNL
                     EVNGLVDKPYSLTYADLKELGPVDVQADMHCVTGWSTLDNTWTGVPFRTLAEKAGVQD
                     DAKWVIAHCDYGYTSDLSLQAMLDDDVLVAWAHGGEPLTGEHGFPLRLVVPKRYAWKS
                     AKWLRGLEFAAQNKRGFWEVRGYHIHAEPWAEERYSYQEGPRAELEP"
     gene            complement(1094..1585)
                     /locus_tag="VIH70_00165"
     CDS             complement(1094..1585)
                     /locus_tag="VIH70_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY8114809.1"
                     /translation="MDATTLTVVIEIPGGSRNKYELDHRTGEIYLDRMLFTATRYPAD
                     YGFIDGTLGGDGDPLDALVFVGEPTFPGCRIQVRPVGMFVMRDEKGQDEKVLCVPLRD
                     PAWSHVMELSDLRPTLLAEIEHFFAVYKDLEDHATETDGFRDRETALEIIREARERAA
                     ARG"
     gene            complement(1610..2431)
                     /locus_tag="VIH70_00170"
     CDS             complement(1610..2431)
                     /locus_tag="VIH70_00170"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEY8114810.1"
                     /translation="MRVGAHLRSGLRTAIPLARETGAEVVQLFISNPRAWSGPRLETA
                     QAFGAEWREAAIGPLFVHAPYLVNIASPNPEFLSKSIELCRRSVAACGVLEAGGFVVH
                     SGAGGEAEVADALDRSATVLQATLAETPHETRLLVELMAGTSGAVASTLGEARTLFDA
                     VDDERLGLVLDTCHLFATGYALDEPEGVEALFAELRELGLADRLRLIHLNDAKFDRGS
                     RRDRHEIVGEGGIGLEGFRAIVTQPEVRDLSVVVETPSSGERRRLELETIRSLAA"
     gene            complement(2428..>3280)
                     /locus_tag="VIH70_00175"
     CDS             complement(2428..>3280)
                     /locus_tag="VIH70_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEY8114811.1"
                     /translation="ALLAIGSAAWASWTYLIPHEVDVPKVVGLTVEHAQVQLDEAGLV
                     VRMTEGRHSTKVPEGSVLAVQPAEGTTLERGDRVKLVPSLGPPPVPVPSLVGLPLADA
                     KSALREGHLKVGEVTRAFSERFDPDRVVRQGVKADAQAPLGSAIDLVVSKGPTPVAVE
                     KVVGLQRDEAVAVLESQGFVVHVEEEFSDRFDEGAVISQSPAKGTDLQPGKTATIVVS
                     KGPPEFPMPNVVGMERDPAVAKLRSLGLLVDVSIVPGHSGMHVVFQEPASGTTVREGD
                     LVHIYVA"
BASE COUNT          500 a         1144 c         1164 g          472 t
ORIGIN      
        1 cgctgatgtt ctgcgtctcg aaggggctcg gggcgccgat cggctcgctc gtctgcggct
       61 cggcggagca gatgcgcgag ggtcgcagga tcaagatcct gttcggtggg gcctggcgcc
      121 aggccggggt cgtcgcggcc gccgggctgg tcgcgctcca ggagggcccg cagcgcctcc
      181 acgaggacca cgagcgggcg cgacgcctcg gggaggccct ggccgagatc gtgcccggca
      241 gcctggatcc ctcggatgtt gaaacgaaca tggtcttcgc cgacacggag gcatcgatgg
      301 cgatgccccc cctccaggtc gcgaaccggc tcgaggaaca tggcgtcggg gcgagcgtgg
      361 tgtcgggcaa ggtccggatg gtcacgcacg tcgacatcga ggatcaggac atcgagacgg
      421 cgatcgcggc ctggcaggcc gcggcagccc gataggaggc tcagccgtga agctgttcgg
      481 cccgaggtat ccggaggaga tcgcgaagcg catcccgccc ggccaacgcc tggtcaagac
      541 gtggccggtc ctgcacttcg gcccgatccc cgacttcgac gagtcgacct ggaacctcga
      601 ggtgaacggc ctcgtggaca agccctactc gctgacgtac gcggacctga aggagctcgg
      661 ccccgtggac gtccaggcgg acatgcactg cgtcaccggg tggtcgacgc tcgacaacac
      721 ctggaccggc gtgccgttcc gaaccctggc cgagaaggcg ggcgtgcagg atgacgccaa
      781 gtgggtgatc gcgcactgcg actacggcta cacgtcggac ctctcgctcc aggcgatgct
      841 ggacgacgac gtgctggtcg cgtgggccca cggcggcgag ccgctcaccg gcgagcacgg
      901 ctttccgctc cgcctcgtcg tcccgaagcg ctacgcctgg aagagcgcga agtggctccg
      961 cgggctcgag ttcgccgcgc agaacaagcg cggcttctgg gaggtgcgcg gctaccacat
     1021 ccacgccgag ccctgggcgg aggagcgcta ctcctaccag gaaggaccgc gcgccgagct
     1081 cgagccgtaa cgcctagcct cgagcggctg ctcgctcccg agcctcccgg atgatctcga
     1141 gcgctgtctc gcgatcgcgg aacccatcgg tctcggtggc gtggtcctcg agatccttgt
     1201 agacggcgaa gaagtgctcg atctccgcga ggagcgtcgg ccgcaggtcg gagagctcca
     1261 tcacgtgcga ccaggcggga tcgcggagcg gcacgcagag cactttctcg tcctgcccct
     1321 tctcgtcgcg catcacgaac atgccgacgg ggcggacctg gatccgacac ccggggaacg
     1381 tgggctcccc gacgaagacg agagcgtcca gcggatcacc atcgccgccg agcgtgccgt
     1441 cgatgaagcc gtagtcggcg ggataccgtg tcgcggtgaa caacatgcgg tcgaggtaga
     1501 tctcgccggt tcggtggtcg agttcgtact tgttgcgcga accgccgggg atctcgatga
     1561 cgacggtcag tgtcgtcgca tccatcgggc cacgatacgt cgaggtgagt caggcggcga
     1621 gcgagcggat cgtctcgagc tcgaggcgcc gacgttcgcc cgatgacggc gtctcgacca
     1681 ctaccgagag gtcgcgcacc tcgggctggg taacgatcgc gcggaacccc tcgaggccga
     1741 tcccgccctc gccgacgatc tcgtgccggt cgcgcctgga gccgcggtcg aacttcgcgt
     1801 cgttcaggtg gatcagccga agacggtcgg ccagcccgag ctcccggagc tccgcgaaca
     1861 gggcctcgac gccctcgggc tcgtcgagcg cgtacccggt cgcgaacagg tgacacgtgt
     1921 ccagcacgag cccgagccgc tcgtcgtcca cggcgtcgaa cagcgtccgc gcctcgccga
     1981 gcgtcgaggc gaccgccccg ctcgtgccgg ccatcagctc gaccagaagc cgggtctcgt
     2041 ggggcgtctc ggctagggtg gcctggagca cggtcgcgga acggtcgagc gcgtcggcga
     2101 cctccgcctc gccgcccgct ccggagtgga cgacgaaccc gcccgcctcg agcacgccgc
     2161 acgccgcgac cgaacgcctg cagagctcga tcgacttcga gaggaactcg gggttcggcg
     2221 acgcgatgtt caccaggtag ggcgcgtgca cgaaaagcgg cccgatcgcg gcctcgcgcc
     2281 actcggcgcc gaacgcctgc gcggtctcca ggcgcgggcc cgaccacgcc cgcgggttgg
     2341 agatgaagag ctggaccacc tcggcgccgg tctctctggc gagcgggatg gcggtacgga
     2401 gcccgcttcg caggtgggcg ccgaccctca cgccacgtag atgtggacga ggtcgccttc
     2461 gcggaccgtg gtcccgctcg ccggctcctg gaacaccacg tgcatgccgc tgtggcccgg
     2521 cacgatcgag acgtcgacga gcagcccgag gctccggagc ttcgcgaccg ccgggtcccg
     2581 ctccatgccg acgacgttcg gcatcgggaa ctcgggcgga cccttggaga cgacgatcgt
     2641 ggcggtcttg cccggctgca ggtcggtgcc ctttgcgggc gactgcgaga tcacggcgcc
     2701 ctcgtcgaag cggtcggaga actcctcctc cacgtggacg acgaacccct gggactccag
     2761 gacggcgacc gcctcgtccc gctgcagacc gacgaccttc tcgacggcga ccggggtcgg
     2821 gcccttgctc accaccaggt cgatcgcgct gccgagcggt gcctgcgcgt ccgccttcac
     2881 gccctgacgt accacccggt cggggtcgaa gcgctcgctg aaggccctcg tgacctcgcc
     2941 gaccttgagg tggccctcgc ggagggcgga cttggcgtcg gcgagcggca gcccgacgag
     3001 gctcgggacg ggcaccggcg ggggaccgag cgacggcacc agcttgacgc ggtcgccgcg
     3061 ttcgagcgtc gttccctcgg cgggttgcac cgcgagcacg ctcccctccg gcaccttggt
     3121 cgagtggcgg ccctccgtca tccgaacgac gagccccgcc tcgtcgagct gcacctgggc
     3181 gtgctcgacg gtgaggccga cgaccttggg gacgtcgacc tcgtgcggga tcaggtacgt
     3241 ccacgaggcc catgccgcgc tgccgatcgc gaggagcgcg
//
LOCUS       DATBRK010000009         9537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_722826, whole genome shotgun sequence.
ACCESSION   DATBRK010000009 DATBRK010000000
VERSION     DATBRK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722826"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="VIH70_00180"
     CDS             <1..1768
                     /locus_tag="VIH70_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017608665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_except=(pos:98..100,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8114812.1"
                     /translation="SCSKATNEMNFVAQKFMRAVLRSNNIDSCNRTUHAPSVVGLATV
                     FGAGGGTSSYEEIENTDLIVLWGSNARETHPIFFHHVLKGIRKGARLVVVDPRRTSSA
                     QWADLWLGIDVGSDVALSNAMAREIIISDLANETFIKRATMRYRAYRDSVMEWTLERG
                     EQATGIPAEVIREFAHDYAKADRAEICWTLGITEHHNAVDNVLALINLGLLCGHVGRY
                     GSGLNPLRGQNNVQGGGDMGAIPNKLPGFQDIEKDPEACARYEEVCGVAVIPKYGWNL
                     TQMFEAMEHGDLTALYVIGENPASSEADVGRARKLMGNLDTLIVQDLFMTKTAQMADV
                     VLPASNAALESEGSVTNSERRVQRVRKVLEPPGNARDDIWIIAQLAKRLGVDWGEVTP
                     RSAWDELRRVSPMHAGMTNERLDEGEGIQWPCWDEEHPGTRFLHARLWEEDRDKRGPK
                     APFSVTPFEPPVDELTEEFPLRLTTGRRLDSYNTGVQTGGYTSPLRRGETIDVSPEDA
                     SRLGVEEGEVVRVTSRRGSLEVPARVDRGLRPGLVFMTLHFPDQVDTNILTIDATDPK
                     SGTAEFKASAVRIEKISESVGV"
     gene            1776..3599
                     /locus_tag="VIH70_00185"
     CDS             1776..3599
                     /locus_tag="VIH70_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY8114813.1"
                     /translation="MDLVLPDALASDEERAAVDAVLGAASSGWSGGERTEAEGRVARG
                     GHEAREQRHMLLPVLHALQSSAGWISPGGLRYACERLTVPPAEAFGVATFYAMFSVEP
                     RPTTVVHVCDDIACRVAGAEGLITQVTSELGSEGEGRIIRSPCLGMCEQAPVAFVQGS
                     GHDTALGHAQGSDLVELARGEKTVLSRTWMRPDHGDLRLLRRIGVVDPSSLDDYRAHG
                     GYEALRRAVELGPEGVIGELKVSKLQGRGGAAFPAGVKWEAVAKQPIRPHYFICNADE
                     SEPGTFKDRVLMEGDPFAVIESLTIAGFATGSEKGFIYIRGEYPNATAMLEQAIDAAY
                     AHGFLGSDVMGEGFAFDVELRRGAGAYICGEETSLFNSIEGKRGEPRNKPPFPVERGV
                     FGKPTGINNVETLVNVIEVLTLGGDAYAEVGTPDSTGPRLFCLSGCVERPGVYEVEHG
                     ITLRELLEMAGGVRGGKELKAILLGGAAGSFVTADDLDLRLTFEDSKAAGVTLGSGVV
                     MVFDEDVDLADIVLRIASFFRDESCGQCVPCRVGTVRQEEALARVIGGRVNGSLTDEL
                     ALIGEIGQVMRDASICGLGQLAANAVDSAIHRLKIFEGGAA"
     gene            3596..4561
                     /locus_tag="VIH70_00190"
     CDS             3596..4561
                     /locus_tag="VIH70_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8114814.1"
                     /translation="MTVVPLAPPTRLIELEVDGRAVRIPEGSTILDALRGEGVDTPTL
                     CFADNLTPVNACRVCVVELEGSRVLVPSCARKAEAGMKVKTDSERVRHSRRMVLEFLG
                     SSVDVGLTGADVERWQTDYGADPGRYGAPAEPAVAGERDARHAGHHHERDGTAAETVH
                     QPVKVDNELYVRDYSKCILCYKCVEACGVDAQNTFAISVAGRGFDARISTEFDVGLPD
                     SACVYCGNCIGVCPTGALMFTSEHEMRETGTWDESRQTVTETICPYCGVGCDLEVHVQ
                     DNSIVKVTSPSDNDVTSGHLCIKGRFGFQFVQNRAPEGPMGDRPA"
     gene            4578..5144
                     /locus_tag="VIH70_00195"
     CDS             4578..5144
                     /locus_tag="VIH70_00195"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEY8114815.1"
                     /translation="MAGGRSTRFGRDKLSEPYRGMPMLQHAVLRLGEVCSEVIVVLAP
                     DAPEPPMPIGAPARFARDPTEGEGPLAGVHAGLLSVEAELALVTGGDMPDLQTRVLLE
                     MLDVAGEAEVDAVALQDRDRFRPLPSVVRTARAVEVAHALLHDGHRALRDLFDGLRLA
                     VIDEPTWVALDPERRTLFDVDAPSDLDG"
     gene            5161..5991
                     /locus_tag="VIH70_00200"
     CDS             5161..5991
                     /locus_tag="VIH70_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8114816.1"
                     /translation="MEAVHEPIRVGRLVVSVVCEGYAPLELSDEMPGAEVDWHAERAR
                     YPWAFVGEDAWPWHVHAFALRTPTGLVLIDAGVSPFPPVRPWAEHTPLEEALAQAEVD
                     PAEVRIVVHTHLHADHAGGSVVDGEPRYPNAVHVVHPADWSRAGGREHPNWARGAMQR
                     LFELGMVDLAAEDHEAWPGVRVVHAPGHTPGHRSAVLRDGDRSMLFTGDLLHTPIQVS
                     RPDHPSNHDVDAEEACRSRVRLVGQARAEGWTVAVSHFARPFGRVGASGWVEEPSPKG
                     "
     gene            6119..7432
                     /locus_tag="VIH70_00205"
     CDS             6119..7432
                     /locus_tag="VIH70_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HEY8114817.1"
                     /translation="MPRRVLIMGAAGRDFHNFNVVYRGDRRYEVVAFTATQIPFIDDR
                     RYPSSLAGEHYPTGIAIHDESELTQLIKDERIDDVVFSYSDVSHEYVMHKASEVLAAG
                     ANFELLGPRDTMLTATVPVVAVCAVRTGSGKSQTTRAIAETLKGAGKRVVAVRHPMPY
                     GNLEAQRVQRYATLEDLDRYDTTIEEREEYEPHITSGTIIYAGVDYGDVLKQAQAECD
                     VLLWDGGNNDLPFYAPDVHVVVADPLRAGHETRYHPGEANLRMANVIVINKMDSASIE
                     QVNALMDTIRRVNPSATVVRANSRVTVDDPHVIGGRRVLVVEDGPTLTHGEMKFGAGV
                     VAARAHGAAEIVDPRPWAVGTIDETFRKYDVGPVLPAMGYSNGQLAEMEKIIDSAEAD
                     VVVIGTPIDLRRVIEIRKPAVRVRYDLEVLPDSPSLLDVLKPVLE"
     gene            7440..8378
                     /gene="arcC"
                     /locus_tag="VIH70_00210"
     CDS             7440..8378
                     /gene="arcC"
                     /locus_tag="VIH70_00210"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEY8114818.1"
                     /translation="MDRVVVALGGNALLRRGQDDTFENLSRSARSAAEGIADIATAGW
                     EVVITHGNGPQVGRILLQQEAAKRWVHPMPLDVCGAESQGLIGYLLQVTIGDVFFERG
                     MERPVATVLTLTRVRADDPAFADPTKPIGPFYEEAEARQHETERGWVLGPDPHGGFRR
                     LVPSPDPYSIVEAPVIRTLVGDGTIVIASGGGGIPVVEEGPRLIGREGVVDKDLAAAI
                     LAREVEASTLLILTDVQAVQRGFGTVRAEAIERMTASEAREMLEAGEFGAGSMGPKVE
                     AAVRFVEQVGGRSAIGDLDAAVSVLSGGSGTSIVSN"
     gene            8391..9077
                     /locus_tag="VIH70_00215"
     CDS             8391..9077
                     /locus_tag="VIH70_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8114819.1"
                     /translation="MRVCVVVYNIRGFRDGTERAVRLIDHARPDVLLLNETGARWRLR
                     RFAKALGMDLAADPWSPLRRRVKDAVLVRPPWRIVAHHQHRFEGGPWLYPRGALAAEL
                     ERGDATLCAISVHLGLHPRERLAHARVLAALAEGVDAPIVAGGDLNERPDGRAVGFLS
                     GCLRDAWLLGGDAEGETFPADQPTARIDYLFVSDGIVVERVLVPPGPDARQASDHRPL
                     VAELTLPGPE"
     gene            9110..>9537
                     /locus_tag="VIH70_00220"
     CDS             9110..>9537
                     /locus_tag="VIH70_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEY8114820.1"
                     /translation="MLSPDIENAMNTQIAAELYSSQLYLAMSAYCEEQALPGFAHWFK
                     LQSDEEREHALKFLRHVVDRGGRVMLTGIDAPQSDFGSPQGAFEQAVAHEMNVTMMID
                     GLYGMAVQHGDYASQVFLQWFVTEQVEEERTAAGVVQMLKT"
BASE COUNT         1458 a         3158 c         3503 g         1418 t
ORIGIN      
        1 cagctgctcg aaggccacca acgagatgaa cttcgtcgcg cagaagttca tgcgggcggt
       61 ccttcgaagc aacaacatcg acagctgcaa ccgcacctga cacgctccct cggtcgtcgg
      121 tctggcgacg gtgttcggag cgggtggggg aaccagttcg tacgaggaga tcgagaacac
      181 cgatctcatc gtgctgtggg ggtcgaatgc gcgcgagacg catccgatct tcttccacca
      241 cgtgctgaag gggatccgca agggcgcccg cctcgtcgtc gtcgaccccc gccggacgag
      301 ctcggcgcag tgggcggatc tctggctcgg gatcgacgtc ggatccgacg tcgccctctc
      361 gaacgcgatg gcgcgcgaga tcatcatctc cgatctggcg aacgagacgt tcatcaagcg
      421 ggcgacgatg cggtaccgcg cctaccgcga ctcggtgatg gagtggacgc tcgaacgcgg
      481 cgagcaggcg accgggatcc ccgccgaggt gatccgggag ttcgcgcacg attacgccaa
      541 ggccgaccgc gcggagatct gctggaccct cggcatcacc gagcaccaca acgccgtcga
      601 caacgtcctg gccctgatca acctggggct gctctgcgga cacgtcggcc gctacgggag
      661 cggcctgaac ccgctccgtg gccagaacaa cgtacagggc ggcggcgaca tgggcgcgat
      721 ccccaacaag ctgccggggt tccaggacat cgagaaggac ccggaggcct gcgcccggta
      781 cgaggaggtc tgcggggtcg ccgtcatccc gaagtacgga tggaacctaa cccagatgtt
      841 cgaggcgatg gagcacggcg atctgaccgc cctctacgtg atcggggaga acccggccag
      901 ctccgaggcc gacgtcggcc gtgcgcgcaa gctcatgggg aacctcgaca cgctcatcgt
      961 ccaggacctg ttcatgacga agaccgcgca gatggcggac gtcgtcctgc cggcgtcgaa
     1021 cgccgccctc gaatcggagg gcagcgtcac gaacagcgag cgccgagtcc agcgcgtccg
     1081 caaggtgctc gagccgccgg gcaacgcgag ggacgacatc tggatcatcg cgcagctcgc
     1141 gaagcggctc ggcgtcgact ggggcgaggt cacgccgcgc tcggcgtggg acgagctccg
     1201 gcgcgtctcg ccgatgcacg ccgggatgac caacgagcgg ttggacgagg gcgaggggat
     1261 ccagtggccc tgctgggacg aggagcaccc cggcacgagg ttcctccacg ctcggctatg
     1321 ggaggaggac cgcgacaagc gcgggccgaa ggcgccgttc agcgtgacgc cgttcgagcc
     1381 gccggtggac gagctcaccg aggagttccc gctccgactg acgaccggcc gccgcctgga
     1441 ctcgtacaac acgggtgtgc agacgggcgg gtacacgtcg cccctgcggc gtggcgagac
     1501 gatcgacgtg tccccggagg acgcctctcg cctcggggtc gaggagggtg aggtcgtccg
     1561 cgtgacctct cggcgtggtt cgctcgaggt tcccgcacgg gtcgatcgcg gtcttcgccc
     1621 cgggctggtg ttcatgacgc tgcacttccc cgaccaggtc gacacgaaca tcctcacgat
     1681 cgacgccacc gacccgaaat ccgggacggc cgagttcaag gcgagcgcgg tccggatcga
     1741 gaagatctcc gagtcggtgg gggtatagcg ccgccgtgga cctggtcctg cccgacgccc
     1801 tcgccagcga cgaggaacgc gccgccgttg acgcggtgct cggcgccgcg agctccggct
     1861 ggtcgggcgg tgagcggacc gaggcggaag ggcgcgtggc ccgcggtggt cacgaggcga
     1921 gggagcagcg gcacatgctg ctcccggtcc tgcacgcgct ccagtcgagc gctggctgga
     1981 tcagccccgg cggactccgg tacgcgtgcg agcgcctcac ggtcccgccc gccgaggcct
     2041 ttggcgtcgc gacgttctac gccatgttct cggtcgaacc acgtccgacg acggtcgtcc
     2101 acgtgtgtga cgacatcgcg tgccgggtag ccggcgccga gggtctgatc acccaggtca
     2161 cttcggaact cggatctgag ggagagggcc ggatcatccg gagcccgtgt ctcggtatgt
     2221 gcgagcaggc tccggttgcg ttcgtgcagg gctcgggcca tgacaccgcc cttggtcacg
     2281 cccaggggtc ggatctcgta gagctcgctc gcggtgagaa gacggtcctg tcgagaacgt
     2341 ggatgcgtcc cgatcatgga gacttgcgcc tccttcggag gataggcgtc gtcgatccgt
     2401 cttcgctaga cgattacagg gcgcacggcg gatacgaggc gctccggcgg gcggtcgagt
     2461 tgggtcccga aggcgtgatc ggcgagctca aggtttcgaa gctccagggg cggggcggcg
     2521 ccgcgttccc ggcgggcgtg aagtgggagg cggtcgccaa acagccgatc cgtccccact
     2581 atttcatctg caacgccgac gaatccgagc ccgggacgtt caaggatcgc gtgctgatgg
     2641 agggcgaccc cttcgccgtc atcgagtcgc tcacgatcgc cggcttcgcc accggttccg
     2701 agaagggctt catctacatc cgaggcgagt acccgaacgc gaccgcgatg ttggagcagg
     2761 cgatcgatgc cgcctacgcg cacggcttcc tcgggtccga cgtgatgggg gaggggttcg
     2821 cgttcgacgt cgagctccgg cgcggcgccg gcgcctacat ctgcggcgag gagacctcgc
     2881 tgttcaactc gatcgagggc aagcgcggcg aaccccgcaa caagccgccg ttccccgtcg
     2941 agcgcggagt attcggtaag ccgaccggga tcaacaatgt ggagacgctc gtgaacgtga
     3001 tcgaggtgct cacgctcggc ggcgatgcgt acgcggaggt cgggacgccc gactcgacgg
     3061 gaccccgcct gttctgtctc tccgggtgcg tcgaacgccc cggcgtgtac gaggtggagc
     3121 acggcatcac gctgcgcgag ctgctggaga tggccggcgg cgtgcggggc ggcaaggaac
     3181 tgaaagcgat cctgctcggc ggcgcggcgg ggtcgttcgt cacggcggac gacctcgacc
     3241 tgcggctcac gttcgaggac tcgaaggccg ccggagtcac gctcggttca ggggtcgtga
     3301 tggtgttcga cgaggacgtg gacctggccg acatcgtcct gcggatcgcg tccttcttcc
     3361 gcgatgagtc gtgcgggcag tgcgtcccgt gccgggtggg aaccgtccgg caggaggagg
     3421 cgctggcccg ggtgatcggg ggacgcgtga acggctcgct caccgatgag ctcgcgctga
     3481 tcggcgagat cgggcaggtg atgcgggacg catcgatctg cggtctgggg cagctggccg
     3541 cgaacgcggt cgactccgcg atccaccgcc tcaagatctt cgaggggggt gccgcgtgac
     3601 ggtcgtcccg ctcgccccgc ccacccgtct gatcgagctc gaggtcgacg gcagggcggt
     3661 ccgcatcccg gagggctcga cgatcctgga cgcgctccgc ggcgagggcg tcgacacgcc
     3721 gaccctgtgt ttcgccgaca acctcacgcc ggtgaacgca tgccgcgtgt gcgtcgtcga
     3781 gctcgagggc tcgcgcgtgc tcgtcccctc gtgcgcccgc aaggccgagg cggggatgaa
     3841 ggtcaagacg gactccgagc gcgtccgcca ctcgcgcagg atggtgctcg agttcctggg
     3901 ttcgagcgtc gacgtcgggc tcacgggtgc cgacgtcgaa cggtggcaga ccgactacgg
     3961 cgccgatccc ggccgctacg gcgcgccggc cgagccagcg gtggcgggcg agcgcgacgc
     4021 gcggcacgcc ggccaccatc acgagcggga cggcactgcg gccgagaccg tccaccagcc
     4081 ggtgaaggtg gacaacgagc tctacgtccg cgactactcc aagtgcatcc tctgctacaa
     4141 gtgcgtcgag gcgtgcggcg tcgacgcgca gaacacgttc gcgatctcgg tggcggggcg
     4201 tgggttcgac gcgcggatct cgaccgagtt cgacgtcggg ctgcccgatt ccgcgtgcgt
     4261 gtactgcggg aactgcatcg gcgtgtgccc gaccggcgcg ctgatgttca cgagcgagca
     4321 cgagatgcgc gagaccggca cctgggacga gtcgcggcag acggtcacgg agacgatctg
     4381 cccctactgc ggagtcggct gcgatctcga ggtccacgtg caagacaact cgatcgtgaa
     4441 ggtgacctcg ccctcggaca acgacgtgac gagcggccac ctgtgcatca aggggcggtt
     4501 cgggttccag ttcgtccaga accgggcgcc cgaagggccg atgggggacc ggcctgcctg
     4561 aggtcgcggg cgtcgtcctg gcgggcgggc gctcgacgcg tttcggccgc gacaagcttt
     4621 cggagcccta ccgcgggatg ccgatgctgc agcacgccgt cctgcgtctg ggcgaggtgt
     4681 gttcggaggt catcgtcgtc cttgcacccg acgcccccga gcccccgatg ccgatcggcg
     4741 ctccggcgcg attcgcgcgt gatccgacag agggcgaggg tccgctggcc ggggtccatg
     4801 ccggattgct ctccgtcgag gccgagctcg cgctggtcac gggcggcgac atgccggacc
     4861 tgcagacccg ggtcctgctc gagatgctgg atgtcgcggg tgaggcggag gtcgacgcgg
     4921 tggcgcttca ggaccgcgat cggttccgcc cgttgccgag cgtcgtccgg accgcgaggg
     4981 cggtcgaggt cgcgcacgcg ctgttgcacg acggacatcg cgcactgcga gatctgttcg
     5041 atgggctccg gcttgcggtg atcgatgagc cgacctgggt ggcgctcgac cccgagcgcc
     5101 ggaccctgtt cgacgtcgac gcgccgtccg acctcgacgg ttgagattgg cggtgcggtg
     5161 atggaggccg tgcacgagcc gatccgggtc ggacgcttgg tggtctcggt cgtctgcgag
     5221 gggtacgcgc cgctcgagct ctcggacgag atgccgggcg ccgaggtcga ctggcacgcc
     5281 gagcgcgctc gctacccgtg ggcattcgtc ggcgaggacg cgtggccgtg gcacgtccac
     5341 gcgttcgcgt tgaggacgcc gaccggtctc gtgctgatcg atgccggcgt gagcccgttc
     5401 ccgcccgtcc gtccgtgggc cgagcacacc ccgctggagg aggcgctcgc ccaggcggag
     5461 gtggacccgg ctgaggttcg gatcgtcgtc cacacgcacc tgcacgcgga tcacgcgggt
     5521 ggctccgtcg tggacggcga gccccgctac ccgaacgccg tgcacgtcgt gcaccccgcg
     5581 gactggtccc gggccggggg gcgggagcat ccgaactggg cccgcggcgc gatgcagagg
     5641 ctgttcgagc tcgggatggt cgatctggcc gccgaggacc acgaggcgtg gccgggtgta
     5701 cgagtggtgc acgcgccggg tcacacgccc ggtcaccgct cggcggtgct cagggatggt
     5761 gataggtcga tgctgttcac cggcgatctg ctgcacaccc cgatccaggt ctcgaggccc
     5821 gaccacccct cgaaccacga cgtcgatgcg gaggaggcgt gccgatcgcg cgtccgtctc
     5881 gtgggtcagg cccgggcaga gggctggacc gtcgcggtga gccacttcgc ccgcccgttc
     5941 gggcgcgtgg gagcgtccgg gtgggtcgag gagccgtcgc cgaagggctg atgccggagg
     6001 ccgcccgacg ggggtagatt cgaccctgaa gagcgggcct cgccggagtc tcgcgtgcgc
     6061 gggccggcga ggttctcccg cgcatcaaac cctcggaccc tcggaaagga ccccggccat
     6121 gccgcgtcgt gtcctcatca tgggagccgc cgggcgcgac ttccacaact tcaacgtcgt
     6181 ctaccgcggc gaccgtcgct acgaggtcgt cgcgttcacc gcgacgcaga tccccttcat
     6241 cgacgatcgg aggtacccgt ccagcctcgc cggcgagcac tacccgaccg ggatcgcgat
     6301 ccacgacgag agcgagctca cccagctgat caaggacgag cgcatcgacg acgtcgtctt
     6361 cagctactcg gacgtgagcc acgagtacgt gatgcacaag gccagcgagg tgctggcggc
     6421 gggcgcgaac ttcgagctgc tcggccctcg cgacacgatg ctgacggcca ccgttcccgt
     6481 cgtcgctgtc tgtgccgtgc ggacgggttc gggcaagagc cagacgaccc gcgcgatcgc
     6541 cgagacgctc aagggcgccg ggaagcgcgt cgtggcggtt cggcatccga tgccgtacgg
     6601 gaacctcgag gcgcagcggg tgcagcgata cgcgaccctc gaggacctcg accgctacga
     6661 caccacgatc gaggagcgcg aggagtacga gccccacatc acctcgggca cgatcatcta
     6721 cgccggcgtc gactacggcg acgtcctcaa gcaggcgcag gccgagtgcg acgtgctgct
     6781 gtgggacggg gggaacaacg atctgccgtt ctacgcgccg gacgtccacg tggtggtcgc
     6841 cgatccgctc cgcgccggcc acgagacccg ctaccacccc ggcgaggcca accttcgcat
     6901 ggccaacgtg atcgtgatca acaagatgga ctcggcgtcg atcgaacagg tgaacgcgct
     6961 gatggacacg atccgacgcg tgaacccgtc cgcgacggtc gtcagggcca acagccgcgt
     7021 gaccgtggac gacccccacg tgatcggcgg caggcgcgtg ctggtggtcg aggacggccc
     7081 gaccctgacg cacggcgaga tgaagttcgg cgcgggggtc gtcgcggcgc gggcccacgg
     7141 cgccgccgag atcgtggacc cgagaccatg ggcggtcggg acgatcgacg agaccttccg
     7201 caagtacgac gtcgggccgg tgctgccggc gatgggctac tcgaacggcc agctcgccga
     7261 gatggagaag atcatcgact cggccgaggc ggacgtcgtg gtgatcggca cgccgatcga
     7321 cctgcgccgg gtgatcgaga tccgcaagcc cgcggttcgc gtcaggtacg acctcgaggt
     7381 gctcccggac tcgccgagcc tgctcgacgt gctgaagccg gtcctcgaat aggtcggcga
     7441 tggaccgggt cgtcgtcgcg ctcggcggga acgcgctgct gcgtcgcggg caggacgaca
     7501 cgttcgagaa cctgtcccgc tccgcccgct cggccgccga gggcatcgcc gacatcgcga
     7561 cggccggctg ggaggtcgtg atcacccacg gcaacggccc gcaggtgggc cggatcctcc
     7621 tgcaacagga ggccgcgaag cggtgggtcc acccgatgcc gctcgacgtc tgcggggcgg
     7681 agagccaggg gctgatcggg tacctgctgc aggtcacgat cggcgacgtg ttcttcgagc
     7741 gagggatgga gcgccccgtg gcgaccgtcc tcacgctcac gcgggtgcgg gccgacgatc
     7801 ccgcgttcgc ggatccgacc aagccgatcg gaccgttcta cgaggaggcc gaggcgaggc
     7861 agcacgagac cgagcgggga tgggtgctcg ggccggaccc gcacggtggg ttccgccgcc
     7921 tcgtcccctc gccggatccg tactccatcg tcgaggcgcc cgtgatccga accctggtcg
     7981 gcgacggcac gatcgtgatc gcctcgggcg ggggcgggat cccggtggtc gaggagggcc
     8041 ccaggctgat cggccgcgag ggcgtggtcg acaaggacct ggccgcggcg atcctcgcgc
     8101 gcgaggtgga ggcctcgacc ttgttgatcc tcaccgacgt gcaggccgtg cagcggggtt
     8161 tcgggaccgt gcgcgcagag gcgatcgagc gcatgaccgc ctccgaggcg cgcgagatgc
     8221 tcgaggcggg ggagttcggt gccggctcga tgggcccgaa ggtcgaagcc gcggttcggt
     8281 tcgtcgagca ggtgggcggg cggtcggcga tcggcgatct cgacgcggcg gtctcggtgc
     8341 tgtcgggggg gtccgggacg agcatcgtct cgaactaggc ggcgtctcgg atgcgtgtgt
     8401 gtgtcgtcgt ctacaacatc cgggggttcc gggacggaac cgagcgggcg gtgcggctca
     8461 tcgaccacgc ccggcccgac gtgctcctgc tgaacgaaac cggagcccgg tggcgcctgc
     8521 gccggttcgc gaaggccctc ggcatggatc tggccgccga cccgtggtcg ccgctccgcc
     8581 ggcgcgtgaa ggatgcggtg ctcgtccggc cgccgtggag gatcgtcgca caccaccagc
     8641 accgcttcga gggggggccg tggctctacc cgcgcggcgc gctcgcagcg gagctcgaac
     8701 gcggcgacgc gacgctctgc gcgatctcgg tgcatctggg gctgcatccg cgtgagcggc
     8761 tcgcgcacgc gagggtgctc gccgcgctgg ccgagggcgt cgacgccccg atcgtggctg
     8821 gcggcgacct gaacgagagg ccggacggcc gcgccgtggg gttcctgtcc ggttgccttc
     8881 gcgacgcctg gctgctcggc ggcgacgcgg agggcgagac cttcccggcc gaccagccga
     8941 cggcccggat cgactacctc ttcgtctccg acgggatcgt ggtcgagcgg gtcctggtgc
     9001 cgcccggccc cgacgcgcgc caggcgtcgg accaccgacc gctcgtcgcc gagctcacgc
     9061 tgcccggtcc ggagtaggct ggacgatcgc tctggaagga ggaccggcga tgctctcacc
     9121 cgacatcgag aacgccatga acacccagat cgccgccgag ctctactcgt cgcagctgta
     9181 cctcgcgatg tccgcctatt gcgaggagca ggcgctgccg gggttcgcgc actggttcaa
     9241 gctgcagtcg gacgaggagc gggagcacgc gctcaagttc ctgcggcacg tcgtcgatcg
     9301 gggcggccgc gtgatgctca cgggcatcga cgcgccccag tcggacttcg gctcgccgca
     9361 gggggccttc gagcaggcgg tcgcgcacga gatgaacgtg acgatgatga tcgacgggct
     9421 ctacggcatg gccgtgcagc acggcgacta cgcgagccag gtcttcctgc agtggttcgt
     9481 gaccgagcag gtggaggagg agcgtaccgc ggccggcgtc gtccagatgc tgaagac
//
LOCUS       DATBRK010000010         7101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_145693, whole genome shotgun sequence.
ACCESSION   DATBRK010000010 DATBRK010000000
VERSION     DATBRK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145693"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VIH70_00225"
     CDS             <1..186
                     /locus_tag="VIH70_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004536324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY8114821.1"
                     /translation="LVCPNDRGELDYQQDPEILVCRTCGYRYPVRDGIPVMLIDEAEK
                     PATGRTARKPAAKKKGR"
     gene            183..1241
                     /locus_tag="VIH70_00230"
     CDS             183..1241
                     /locus_tag="VIH70_00230"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEY8114822.1"
                     /translation="MTVLDDPAALRAADPGGMLDLVTRLGDHCRVGYALGLAVRPLPE
                     PDGITSIVVCGMGGSAIAGDVVAALAAPRLRLPVLTVRAPQLPEFCGPHTVVVASSYS
                     GDTAETLALFEEAVARGCRLVAITAGGELDRRAGELGTGRVIVPGGLMPRAAFGYLSL
                     GTLGTLEAMGIVPTFADDVEESIEELAGVVSDAGPDVPPVSNPAKELARAIGDRVPVV
                     WGAEGIGAVAATRWTSQFNENAKVPSFAAALPELDHNQVVGWSADRGTGFAVIGLRHP
                     GEHGDVAARFPLSLEIARSSGASVHEVASRGRSELARLLTLVQLGDLVSTYLGIVRGV
                     DPSPIEAIVNLKRALAEA"
     gene            1238..2101
                     /locus_tag="VIH70_00235"
     CDS             1238..2101
                     /locus_tag="VIH70_00235"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY8114823.1"
                     /translation="MSRVRTLPSPGDGVSERIAAAIRERSPLEPVVGVVLGSGLSGAV
                     ETVRAAALGEQAELSYADLPGFPTTTVMGHAGQLWLGTVGDRGVAIFQGRIHLYEGHG
                     MALASITSRVAAELGARAMVLTAATGSVDPDVEAGTLVVLRDHLNLMGQNVLTGWRYP
                     DGSPAFVDVSRVYDPELADLALASGRAHLGGTDRAGRIREGVYAALPGPSYETPAETR
                     MLRQLGATVVGMSTVPEAVAARALGMRVLGLAFATNAAGVEVSHEEVLAASKAAAGTI
                     GRVILDLVERF"
     gene            2135..3394
                     /gene="ahcY"
                     /locus_tag="VIH70_00240"
     CDS             2135..3394
                     /gene="ahcY"
                     /locus_tag="VIH70_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY8114824.1"
                     /translation="MDCDVRDLGLAQGGRERMEWAAGEMGVLALIRARFEKERPLEDI
                     RIAACLHVTTETANLVETLTAGGAEVSLCASNPLSTQDDVAAALCEQSDVGVYAIRGE
                     DNETYYRHIEAVLDRRPQITMDDGADLVSLLHRERADQVSDVIGGTEETTTGVIRLRA
                     MAVDGALRYPIVSVNDANTKHLFDNRFGTGQSTVDAIMRATNLLLAGRTFVVAGYGMC
                     GRGVASRARGMGAHVVVTEVDPLPALEAAMEGYRVLPLREAARIGDVFVTVTGDTSVI
                     RREHLEVMKDGAVLANAGHFDVEIEKEALAELSGGRVRRIRGYVDEYTMGDGRRLHLL
                     GEGRLVNLAVAEGHPAAVMDMSFANQALSVEWIVKGRGALAPGVYPVPTQIDEEVAKL
                     KLQAMGVEIDALTAEQRDYLSSWQQGT"
     gene            3420..4064
                     /locus_tag="VIH70_00245"
     CDS             3420..4064
                     /locus_tag="VIH70_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114825.1"
                     /translation="MSDPISVRARFERFPATVKGAFILRGEDANPHQVVVGGARVAAL
                     GAGGSSPVPMAPVTLDVAPHRDVFVPFELPLSELEPGWYTLVCDVDVDGTPATYDGGR
                     RFSVPWPRATVRRGAVKVGRQVKLEGTTIHVEQVDCTGDSIKVHLRAEPAAELTLKLF
                     ADGRRLQVLELELDEVTGRGRATAYPLMRSDASLRVELKGRGKGSEAAVDIPLP"
     gene            4100..4897
                     /locus_tag="VIH70_00250"
     CDS             4100..4897
                     /locus_tag="VIH70_00250"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY8114826.1"
                     /translation="MPAPFLDVLFPKRCAGCGSGPWPFCSACRSELVVLSPPWCERCG
                     APASRPVASCRDCPPDAIAIARAPFGFVGPIRRAIHRLKFGGWHPVADALGVAMTEAW
                     ADIPWTPGALTWVPLSRQRLAARGYDQARALARAVAPRLGVPAVPLVRRVRDPGPQAR
                     RGAAARREAMRGVFEAEGASGGVLLVDDVLTTGATGGACATALLEAGAEAVGLLTAAR
                     AVSSARRPRPILGHGLASGSVVAPGNRSPAVDASRGRSDPRKPTLGR"
     gene            5164..5439
                     /gene="raiA"
                     /locus_tag="VIH70_00255"
                     /pseudo
     CDS             5164..5439
                     /gene="raiA"
                     /locus_tag="VIH70_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
     gene            5560..6270
                     /locus_tag="VIH70_00260"
     CDS             5560..6270
                     /locus_tag="VIH70_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8114827.1"
                     /translation="MKAETVKAETGKAEPGSEKLRVLVCDDHALFRRGLKMVLDEEPD
                     LELVGEASDGAEVVVRAQELMPDVILMDVRMPKRSGIEAAGEIKDSLPHVKILMLTIS
                     DDEADLYEAIKAGASGYLLKEIPIEEVADAIRSVWAGQSRISPSMASKLLSEFAAMSK
                     SSDEKPQMPAPKLTDREMEVLRLVAQGLNNRDIAGKLFISENTVKNHIRNILEKLHLH
                     SRMEAVVYAVREKMIEIT"
     gene            6412..>7101
                     /locus_tag="VIH70_00265"
     CDS             6412..>7101
                     /locus_tag="VIH70_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY8114828.1"
                     /translation="MVIEKLGQFGEGRKKKQLAQIAGLVNTYEPEVEDLSDAELAGKT
                     AEFRERLEAGETLDDLLPEAFASVREAARRTIGQRHFDVQVMGAVALHQGNIAEMKTG
                     EGKTLTSTMAAYLNSLADGVHVVTVNDYLAKRDSEWMGPIYRALGVSVGLIQSQMSPS
                     ERRPAYAADITYGTNNEFGFDYLRDNMAMRPEDMVQRGHAYAIVDEVDSILIDEARTP
                     LIISGMVADSAK"
BASE COUNT         1018 a         2340 c         2745 g          998 t
ORIGIN      
        1 ctggtgtgcc cgaacgatcg gggcgagctc gactaccagc aggatcccga gatcctcgtg
       61 tgccggacgt gcgggtaccg gtaccccgtg cgcgacggca tccccgtcat gctgatcgac
      121 gaggccgaga agcccgcgac cggtcggacc gcgcgcaaac ccgcggccaa gaagaagggc
      181 cggtgaccgt cctcgacgac cccgcggcgc tgcgcgccgc cgacccgggc gggatgctcg
      241 acctcgtcac gcgcctgggt gaccactgtc gagtgggata cgcgctcggc ctcgcggtgc
      301 gaccccttcc cgaacccgac gggatcacct ccatcgtggt gtgcgggatg ggggggtcgg
      361 cgatcgccgg cgacgtcgtc gccgcgctcg cggcgccgcg cctgcggctg ccggtgctca
      421 cggttcgagc gccgcagctg cccgagttct gcgggcctca cacggtggtc gtcgcctcgt
      481 cgtactcggg tgacacggcg gagacgctcg cgctgttcga ggaggcggtc gcccgggggt
      541 gtcggctcgt cgccatcacc gcgggcggcg agctcgaccg tcgcgccggg gagctcggga
      601 ccggccgcgt gatcgtgccc ggcggcctga tgccgcgggc ggcgttcggc tacctgtcgc
      661 tcgggacgct cggcacgctc gaggcgatgg ggatcgtgcc gacgttcgcc gacgacgtcg
      721 aggagtcgat cgaggagctg gccggcgtgg tctcggacgc ggggcccgac gtgccgccgg
      781 tgtcgaaccc ggccaaggag ctcgcgcgcg cgatcggcga tcgcgtcccc gtcgtctggg
      841 gcgccgaggg gatcggcgcg gtggcggcga cccgctggac gtcccagttc aacgagaacg
      901 ccaaggtgcc ctcgttcgcc gccgccctgc ccgagctcga tcacaaccag gtcgtcggat
      961 ggtcggcgga tcgcgggacc gggttcgcgg tgatcggcct ccgtcacccc ggcgagcacg
     1021 gcgacgtcgc cgcacgcttc ccgctctcgc tcgagatcgc gcggagctcg ggcgcctcgg
     1081 tccacgaggt cgcgagccgg gggcgttcgg agctcgcgag gctgctgacg ctcgtgcagc
     1141 tcggcgacct ggtgtccacg tacctgggca tcgttcgcgg cgtcgatccg tcgccgatcg
     1201 aggcgatcgt caacctgaag cgcgcgctgg ccgaggcatg agccgggtcc gaacgctgcc
     1261 atcacccggg gacggggtct cggagcggat cgccgcggcg atccgcgagc gatcgccgct
     1321 cgagccggtc gtcggggtcg tgctcgggtc ggggctctcg ggggcggtgg agacggtgcg
     1381 cgccgcggcg ctcggcgagc aggcggagct ctcgtacgcg gatctccccg ggttcccgac
     1441 gacgacggtg atgggtcacg cggggcagct gtggctcgga acggtcggcg accggggggt
     1501 cgcgatcttc cagggccgga tccacctcta cgagggccac gggatggcgc tggcctcgat
     1561 cacgtcgcgc gtggcggccg agctcggggc ccgcgcgatg gtgctcaccg cggcgacggg
     1621 gtcggtggat cccgacgtcg aggccgggac gctggtcgtc ctgcgggacc acctgaacct
     1681 gatggggcag aacgtcctca ccgggtggcg gtacccggac ggctcgccgg cgttcgtcga
     1741 cgtctccagg gtgtacgacc ccgagctggc ggacctggcg ctcgcctcgg ggcgggcgca
     1801 ccttgggggg acggaccggg ccggtcgcat ccgcgagggc gtgtacgcgg cgctccccgg
     1861 gccgtcgtac gagacgcccg ccgagacccg catgctgcgg cagctggggg caacggtcgt
     1921 cgggatgtcg acggtccccg aggccgtcgc cgcccgggcc ctcgggatgc gcgtgctggg
     1981 cctcgcgttc gcgaccaacg ccgcgggggt ggaggtctcc cacgaggagg tcttggccgc
     2041 gtcgaaggcc gcggccggga cgatcgggcg ggtcatcctt gacctcgtgg aacgcttctg
     2101 acgtggatcg cacagggaag gggaaggtgg gcgcatggat tgcgacgtga gggacctggg
     2161 cctggcgcag gggggccgcg aacggatgga gtgggccgcg ggtgagatgg gcgtgctggc
     2221 gctgatacgg gcgcggttcg agaaggaacg tcccctggag gacatccgga tcgcggcctg
     2281 cctccacgtc acgaccgaga ccgcgaatct cgtggagacg ctcacggcgg gcggggccga
     2341 ggtgtccctg tgcgcgtcga acccgctctc gacccaggac gacgtcgccg ccgcgctgtg
     2401 cgagcagagc gacgtcggcg tctacgcgat caggggcgag gacaacgaga cgtactaccg
     2461 gcacatcgag gcggtgctcg atcggcgccc acagatcacg atggacgacg gcgcggacct
     2521 cgtgtcgctg ctgcaccgcg agcgcgccga ccaggtctcg gacgtgatcg gcgggacgga
     2581 ggagaccacg accggcgtga tccgcctccg tgcgatggcc gtggacggag cgttgcgcta
     2641 tccgatcgtg tcggtcaacg acgccaacac gaagcacctg ttcgacaacc ggttcggcac
     2701 ggggcagtcg acggtcgacg cgatcatgcg cgcgaccaac ctcctgctcg cgggtcgcac
     2761 gttcgtcgtg gccggctacg ggatgtgcgg gcgcggcgtg gcctcgaggg cgagggggat
     2821 gggcgcgcac gtcgtcgtca ccgaggtcga tccgctgccg gcgctggagg ccgcgatgga
     2881 gggctaccgc gtcctcccgc tgcgcgaggc cgcccggatc ggcgacgtgt tcgtcacggt
     2941 caccggcgac acgagcgtga tccggcgcga gcatctcgag gtcatgaagg acggcgcggt
     3001 cctcgcgaac gccggtcact tcgacgtcga gatcgagaag gaagcgctcg ccgagctctc
     3061 gggaggtcgc gtgcggcgga tccgagggta cgtggacgag tacacgatgg gggacggccg
     3121 ccgtctgcat ctcctcggcg agggtcgcct cgtgaacctc gcggtggcgg agggccatcc
     3181 cgcggcggtg atggacatgt cgttcgcgaa ccaggccctg tcggtcgagt ggatcgtcaa
     3241 gggccgcggg gcgctggctc ccggcgtgta cccggtgccg acccagatcg acgaggaggt
     3301 cgcgaagctg aagctccagg cgatgggggt ggagatcgac gcgctcacgg ccgagcagcg
     3361 ggactatctg agctcgtggc agcagggaac ctgatcatcg tgtcggtggc ccgcgcccgg
     3421 tgagcgaccc gatctcggtc cgcgcccggt tcgagcgctt cccggctacg gtcaagggtg
     3481 cgttcatcct gcgcggcgag gacgcgaacc cgcaccaggt ggtggtcgga ggcgcacgcg
     3541 tcgcggcgct gggggccggc ggctcgagcc cggtaccgat ggcgcccgtg accctcgatg
     3601 tcgccccgca ccgggacgtc ttcgtcccgt tcgagctccc gctgtccgag ctcgaacccg
     3661 gctggtacac gctcgtgtgc gacgtcgacg tcgacgggac gcccgcaacg tacgacgggg
     3721 gccggcgctt ctcggtcccg tggccgcggg ccaccgtgcg ccgtggtgcg gtgaaggtcg
     3781 gtcgtcaggt caagctcgag gggacgacga tccacgtcga acaggtggac tgcaccggcg
     3841 actcgatcaa ggtgcacctg cgcgccgagc ccgccgcgga gctcacgttg aagctgttcg
     3901 ccgacggccg tcggctgcag gtgctcgagc tggaactcga cgaggtgacc ggacgcggca
     3961 gggcgacggc gtacccgttg atgcggtccg acgcgtcgct tcgggtcgag ctcaaggggc
     4021 gaggcaaggg ttcggaggcg gcggtcgaca tcccgctgcc gtgacgccgg gtgtcggtgg
     4081 cgcggggtag cgtccgcgcg tgccggcccc gttcctcgac gtgctgttcc ccaagcgatg
     4141 tgcggggtgc ggttcgggac cgtggccgtt ctgctccgcg tgccgttcgg agctggtggt
     4201 gctctcgcct ccgtggtgcg agcgatgcgg ggcgccggcg tcgcgccccg tcgcctcgtg
     4261 ccgggactgt ccgcccgacg cgatcgcgat cgcccgggcg ccgttcgggt tcgtcgggcc
     4321 gatccgtcgc gcgatccacc ggttgaagtt cggcgggtgg caccccgtcg cggatgcgct
     4381 cggggtcgcg atgaccgagg cgtgggccga catcccctgg acgcccggcg ccctcacctg
     4441 ggtgccgctg tcccgacagc ggctggcggc ccggggctac gaccaggccc gggcgctcgc
     4501 gcgggcggtc gcacccaggc tgggcgtgcc ggccgtcccg ctcgtgcggc gcgtgcggga
     4561 cccgggaccg caggctcgac gcggggccgc cgcgcggcgc gaggcgatgc gcggcgtgtt
     4621 cgaggcggaa ggcgcctcgg gcggtgtcct gctcgtcgac gacgtgctca ccacgggcgc
     4681 caccggggga gcgtgcgcca ccgcgctgct cgaggccggg gccgaggcgg tggggttgct
     4741 cacggcggcc cgggccgtgt cctcggcgcg gcggccccgg cctatactcg gccacgggct
     4801 cgcgtccggg tctgtggttg ccccggggaa ccgatccccg gcagtcgatg ccagccgcgg
     4861 gcgaagcgac ccacgtaagc cgacccttgg tcggtgagca tggcgcggtt tagatgtaag
     4921 tcctgccccg tgggggagac ctcggcggga gagggtgcga agccttccga tgggcggcga
     4981 tcactccggc aggcggatgt ggggccaaag accaggtcag ccggacgcgg gcctctgcgc
     5041 tcggggcaga cgagcgcgga gggtcggtgt cggggtacgt tccgggtggg acggagtgtt
     5101 cggggtggac ggagtgagga gcggtcccgc cggagcggcc gggggaacgc ccaggggaag
     5161 tggatggagt tcgtcctcat aggccggggg gagaccgtac ggcgcctccg gcacgtgacc
     5221 gagcacaagc tcggcaggct cgagcggatc gagccccgga tcacgcgcct cgaggtcgag
     5281 gtgatcgccg agaagaaccc gcggcgcggc ggcatccacc gtgtggaggc ggtcgccgcg
     5341 gcgccacgca agtccttccg cgcccacgcc gaggcgaagg acgtggagtc ggcgctcgac
     5401 gtggtggcgg agcggctcga gcgccagatc cgtgaccacc acgaacgtag gctggctttg
     5461 cggaacgcgg gtgccgttcg cgtaaaatcc ggccagccgc ccggcgggac ccgccgagcc
     5521 agaggtcagg agcgcggagt gaccgacaag gccgaggccg tgaaggccga gaccgtgaag
     5581 gccgagaccg gcaaggcgga gccaggctcg gagaagctcc gggtgctcgt gtgcgatgac
     5641 catgccctgt tccggcgggg gttgaagatg gtgctcgacg aggagcccga tctcgagctc
     5701 gtcggcgagg catcggacgg ggccgaggtg gtcgttcgcg cccaggagct catgccggac
     5761 gtgatcctga tggacgtccg gatgccgaag cgctccggga tcgaggccgc cggcgagatc
     5821 aaggactcgc tgccgcacgt caagatcctg atgctcacga tcagcgacga cgaggcggac
     5881 ctgtacgagg cgatcaaggc gggcgcctcc ggctacctgc tgaaggagat cccgatcgag
     5941 gaggtcgccg acgcgatccg ttcggtgtgg gcggggcagt ctcggatcag cccgtcgatg
     6001 gcgagcaagc tgctgtcgga attcgcggcg atgagcaagt cctcggacga gaagccccag
     6061 atgccggcgc ccaagctcac cgaccgtgag atggaggtgc tgcggctcgt cgcccagggg
     6121 ctgaacaatc gggacatcgc cgggaagctg ttcatctccg agaacacggt gaagaaccac
     6181 atccgcaaca tcctggagaa gctccacctc cactcgcgca tggaggcggt cgtctacgcc
     6241 gtgcgcgaga agatgatcga gatcacgtag cgccgtcgcc ggatgagggc ctcgccgccg
     6301 ccggcatccg gcccaggccc gcgacccgga gactgaggcg ccgggaggat cggtcaggtt
     6361 cgagacctcc ggtaccatag gggttccaac ccctccacac cgaggcgaag catggtcatc
     6421 gagaagctcg gccagttcgg cgaaggccgc aagaagaagc agctcgcgca gatcgcaggg
     6481 ctggtcaaca cgtacgagcc cgaggtcgag gacctttccg acgccgagct cgccggcaag
     6541 accgccgagt tccgcgaacg gctcgaggcg ggggagacgc tggacgacct gctgcccgag
     6601 gcgttcgcgt ccgtccggga ggccgcgagg aggacgatcg ggcagcggca cttcgacgtc
     6661 caggtgatgg gcgcggtcgc gctccaccag ggcaacatcg ccgagatgaa gaccggtgag
     6721 ggcaagaccc tcacctcgac gatggcggcc tacctcaact ccctcgccga cggcgtgcac
     6781 gtcgtcacgg tcaacgacta cctggccaag cgcgactcgg agtggatggg cccgatctac
     6841 cgggcgctcg gcgtctcggt ggggctgatc cagtcccaga tgtcgccgtc cgaacgccgc
     6901 ccggcgtatg cggccgatat cacgtacggg accaacaacg agttcgggtt cgattacctg
     6961 cgcgacaaca tggcgatgcg cccggaggac atggtgcagc gtggccacgc gtacgcgatc
     7021 gtggacgagg tcgactcgat cctgatcgac gaggctcgga ccccgctgat catcagcggc
     7081 atggtggccg acagcgcgaa g
//
LOCUS       DATBRK010000011         5203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1589384, whole genome shotgun sequence.
ACCESSION   DATBRK010000011 DATBRK010000000
VERSION     DATBRK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589384"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /gene="ffs"
                     /locus_tag="VIH70_00270"
     ncRNA           complement(<1..121)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIH70_00270"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            257..955
                     /locus_tag="VIH70_00275"
     CDS             257..955
                     /locus_tag="VIH70_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEY8114829.1"
                     /translation="MLAIRALLVDFDGTACLQDVSELLLDAFGEPGWERFDDAVGRGE
                     MGLREAADHQVAILHGSRDEMLAFALERAELAPTFVPFAAWAEERRLPLELASDGFAF
                     YIRPILERAGLGNLEVVTNDFVPRNGGASLGHPNGHPECIGCGTCKMLAATRLRERHG
                     PIAFVGEGQSDRYGALYSDLVFAKDALVEICEQDRMPYLRWQTFDDVRQVLEEADTLP
                     GPVGAERCPGWRTS"
     gene            complement(1010..1096)
                     /locus_tag="VIH70_00280"
     tRNA            complement(1010..1096)
                     /locus_tag="VIH70_00280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1060..1062),aa:Ser,seq:gga)
     gene            complement(1135..3300)
                     /locus_tag="VIH70_00285"
     CDS             complement(1135..3300)
                     /locus_tag="VIH70_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8114830.1"
                     /translation="MSRTLAHTGAEGASGQSPARRDDSAGAEILERFPLAMYAVGADG
                     STGHISPALQRVLGFPQESSNWQCVNLTELLHPDDRDRVLRKLAAGRESLEPFRMEYR
                     LQRYDGAYVWVLDEAVFIRDARGDTIREGFLVDITDRVQALEDLSVSEERFRTLLSNI
                     PGAMYRCDLDADWDMEFISDNIEAISGYPASDFIQSRVRTFASIIHPDDRQAVERIVG
                     EAVSREEPFILEYRIVGADGGVRWVYEKGQGVFGPGGTVLWLDGAIFDITERKLLQEQ
                     LATQAFHDSLTGLANRALFRDRADHALARVARHGGGLAVLLLDLDGFKFINDSMGHQV
                     GDEVLVTVAKRILTSSRTSDTVARLGGDEFVVLLEEDVIEPAAIIVAQRILGVLRETF
                     MCQGREVSLGGSIGIAFTSGADAMSTDDLLRNADAAMYCAKANGKDRYELFEPSMHTK
                     AVERFELITQMQRGLERGEFVLHYQPIWELADRTLTGVEALVRWNHPSRGLLPPGEFI
                     AAAEQTGFIVPLGNWVLDESCRQVRAWQEAFPGRPLSVSVNITPRQLIGSDLVGRVRD
                     ALLLHELDPASLTLEITEGALLHDVEVTKRKLDELKALGVRLAIDDFGTGSSMLGNLR
                     RFPLDELKIDRSFVIGMTDPAEGSVVRAILELADAMHMNVVAEGIELPEQLTRLRESG
                     CSNGQGFLFAKPAAPDEIGSLLGRLDPASTTTDTVASNS"
     gene            complement(3415..3504)
                     /locus_tag="VIH70_00290"
     tRNA            complement(3415..3504)
                     /locus_tag="VIH70_00290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3468..3470),aa:Ser,seq:cga)
     gene            3593..4006
                     /locus_tag="VIH70_00295"
     CDS             3593..4006
                     /locus_tag="VIH70_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8114831.1"
                     /translation="MKAERLRKIPLFAGLSDHQYERLERWTDEIDVPTNKHLVNQGAY
                     PHEFMVIETGTAEVTHDGEHLADLGPGDFFGEMALLLNVPRTATVEATSEVTLVVMHD
                     RNFRAMEEEIPAVAKRVNAIMEERRAKSAEQGIDT"
     gene            complement(4227..4682)
                     /gene="tadA"
                     /locus_tag="VIH70_00300"
     CDS             complement(4227..4682)
                     /gene="tadA"
                     /locus_tag="VIH70_00300"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY8114832.1"
                     /translation="MDAPDERIMRLALEAARAAAAGGDVPVGAVIVRGDEILARAGNA
                     RERELDPTAHAEILALRRASGAIGSWHLEGCAMVVTLEPCAMCAGAIVLARLDRLVFG
                     ASDPKAGFAGSLGNLVRDGRLNHEVDVTVGVLADECGEVLRAFFADRRS"
     gene            complement(4686..5000)
                     /locus_tag="VIH70_00305"
     CDS             complement(4686..5000)
                     /locus_tag="VIH70_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY8114833.1"
                     /translation="MEAVDSLPPWVRERLENIELILEDRPPRGEPNLLGRYHGVPLTR
                     RGQFQSGPTFPDTITLYQRTIERVARDEEHLRQVIAHTVEHEVAHFFGISDDRLREID
                     RY"
     gene            complement(5036..5194)
                     /locus_tag="VIH70_00310"
     CDS             complement(5036..5194)
                     /locus_tag="VIH70_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY8114834.1"
                     /translation="MLRAVRRIVAAVELAGMDVVEVSPPYDQSEVTSMLAYRVVMEAL
                     SSLAARRG"
BASE COUNT          833 a         1835 c         1738 g          797 t
ORIGIN      
        1 acccgacctt cgacgccccg taccgggagc gactgcggac ccgccgggcc tcggacgggg
       61 attcagcccc gctgaagcgg attgcgggtt cagggcaccg ctggctcccc gcctagcacg
      121 gccacctcgg cgagtatacg caccgccccg acacgactga ccccgtcggg tcaacccgcg
      181 agcgccaccc acacggcgtc gcgcgtgatc tgcccaccga tcgcgaagga ggcgagggcg
      241 caggtaccct cccccgatgc tcgccatcag agcgctcctg gtggatttcg acggcactgc
      301 ctgcctgcag gacgtgtccg agctgctgct ggatgcgttc ggcgagccgg ggtgggaacg
      361 gttcgacgac gcggtcggcc gcggcgagat gggcctgcga gaggcggccg accaccaggt
      421 ggcgatcctg cacgggtcgc gcgacgagat gctcgccttc gcgctcgaac gcgccgagct
      481 cgcgccgacc ttcgtgccgt tcgccgcatg ggccgaggag cgacgcctgc cgctggagct
      541 cgcgtccgat gggttcgcct tctacatccg gccgatcctg gaacgcgccg ggctcgggaa
      601 tctagaggta gtcaccaacg acttcgttcc ccggaacggg ggcgcgtccc tcggtcaccc
      661 gaacggccac cccgagtgca tcggctgcgg gacctgcaag atgctggccg cgaccaggct
      721 ccgggagcgg cacggaccga tcgcgttcgt cggcgagggt cagtcggacc ggtacggggc
      781 gctctactcg gacctggtgt tcgcgaagga cgcgctcgtc gagatctgcg agcaggaccg
      841 catgccctac ctgcgatggc agacgttcga cgacgtgcgg caggtgctcg aggaggcgga
      901 cacgctcccg ggccccgtcg gcgccgaacg ctgtcccgga tggcggacct cctgatcgag
      961 ccgccgcatg tatgtgcgat acgcgaagcc gctcccatgg gcggctgagc ggtggccgcg
     1021 ggattcgaac ccgcgaggcg gttgcccacc tacacgctct ccaggcgtgc cccttcgtcc
     1081 gctcgggcag gccaccgcgg tcgagtctag cgaccgccgc gggccgagct cgtgctacga
     1141 gttcgacgcc accgtatccg tcgtggtaga cgccgggtcg agccgtccga gcaacgatcc
     1201 gatctcgtcg ggggcggccg gcttcgcgaa caggaagcct tgtccgttcg agcagcccga
     1261 ttcccggagc cgtgtgagct gctccgggag ctcgatccct tcggcgacga cgttcatgtg
     1321 catcgcatcg gcgagctcga ggatggcccg aaccacgctc ccctcggccg ggtcggtcat
     1381 cccgatcacg aacgaccgat cgatcttcag ctcgtccagc gggaaccggc ggaggttgcc
     1441 gagcatggac gacccggtgc cgaagtcgtc gatcgcgagc ctgacgccga gcgccttcag
     1501 ctcgtcgagc ttgcgcttcg tcacctcgac atcgtgcagg agcgcccctt cggtgatctc
     1561 gagcgtgagc gatgccgggt cgagctcgtg cagcaggagt gcatcacgga cgcggccgac
     1621 gaggtcggat ccgatgagct ggcgtggcgt gatgttgacg ctcaccgaga gcgggcggcc
     1681 ggggaacgcc tcctgccatg ctcgaacctg acgacaggac tcgtccagta cccagttccc
     1741 gaggggaacg atgaaccccg tctgctcggc cgcggcgatg aactcgcccg gcgggaggag
     1801 tccgcggctc ggatggttcc agcgcaccag ggcttcgaca ccggtgagcg tccgatcggc
     1861 gagctcccag atcggctggt agtggagcac gaactcgccg cgttccagac cgcgttgcat
     1921 ctgggtgatg agctcgaatc gctcgaccgc cttggtgtgc atcgagggct cgaacagctc
     1981 gtagcggtcc ttcccgttcg ccttggcgca atacatcgcc gcgtcggcgt tgcgcaggag
     2041 gtcgtcggtg gacatggcgt cggctcccga ggtgaaggcg atgccgatgc ttcccccgag
     2101 cgagacctcc cggccctggc acatgaacgt ttcccggagg acgcccagga tccgctgggc
     2161 gacgatgatg gcggccggtt cgatcacatc ctcttcgagc aggacgacga actcgtcgcc
     2221 gccgagcctg gccaccgtgt cgctcgtgcg actcgacgtc aggatccgct tggcgaccgt
     2281 cacgagcacc tcgtcgccga cctggtgacc catgctgtcg ttgatgaact tgaacccgtc
     2341 gagatcgagc agcaacacgg cgagcccacc cccgtggcga gcgacacgcg ccagcgcatg
     2401 gtccgctcgg tcccggaaca gcgcgcggtt cgcgagcccc gtcagcgagt cgtggaacgc
     2461 ctgcgtcgcc agctgctcct ggagcaactt ccgctccgtg atgtcgaaga tggctccgtc
     2521 caaccagagc accgtgcctc cggggccgaa gacgccctgg cccttctcgt acacccagcg
     2581 gaccccaccg tcggcgccga cgatccggta ctccaggatg aacggctcct cccgcgacac
     2641 ggcctccccc acgatccgct cgacggcttg ccgatcgtcg gggtggatga tcgacgcgaa
     2701 cgtccgaacg cggctctgga tgaagtccga cgccgggtag cccgagatcg cctcgatgtt
     2761 gtcgctgatg aactccatgt cccaatcggc gtcgaggtcg caccgataca tcgcccccgg
     2821 gatgttcgac agcagggtcc ggaaccgctc ctcgctgacg ctcagatcct cgagggcctg
     2881 tacgcgatcc gtgatgtcga ccaggaaccc ctcccggatc gtgtcgccgc gtgcatctcg
     2941 gatgaacacc gcttcgtcga gcacccagac gtacgctccg tcgtaccgct ggagccggta
     3001 ctccatgcga aagggctcga gcgactcacg tccggcggcg agtttccgca ggacgcgatc
     3061 gcggtcgtcg ggatggagca gctcggtgag gttcacgcac tgccagttcg aggactcctg
     3121 cgggaagccc agcacccgct gcagggcggg gctgatatga cccgtcgacc cgtcggctcc
     3181 gacggcgtac atcgccaggg ggaagcgctc gaggatctcc gcgccggccg agtcgtcccg
     3241 acgggcaggg ctctgtccgg acgccccctc ggcgcccgtg tgggcaagcg ttcgcgacat
     3301 atatgtaggt atcgacttcg ccgctctgcg cttgaagcgg ggtttccagg caggagcctc
     3361 ggatcgaacg acggggacgg tggccgcccc ttccgaggcg gccggctggt ctcgcggagg
     3421 cgacaggatt cgaacctgtg aggggcggat taggccccta accgctttcg aggcggtccc
     3481 gttcgtccgc tccggcacgc ctccgggccg agtctagcaa gcggcctcac gccggcctgt
     3541 gcgaggattc gactcggatc gagggagcgc cacggagaac ggaggaagcc ccatgaaggc
     3601 cgagcgtctg cggaagatcc ccctattcgc ggggctgagc gaccaccagt acgagcgact
     3661 cgaacgctgg accgacgaga tcgacgtccc cacgaacaag cacctggtga accagggggc
     3721 ctacccgcac gagttcatgg tgatcgagac cggcacggcg gaggtcacgc acgacgggga
     3781 gcacctcgcg gatctcgggc ccggcgactt cttcggcgag atggcgctgc tgctcaacgt
     3841 gccccgaacc gccaccgtcg aggcgacctc cgaggtcacc ctcgtcgtca tgcacgaccg
     3901 gaacttccgc gcgatggagg aggagatccc cgcggtcgcc aagcgcgtga acgccatcat
     3961 ggaggagcgt cgagccaagt cggcggagca ggggatcgac acgtagaggg ggagtcgccg
     4021 gcctcttcgg ggctggtgat gcggtcggtt ttctggcggc cgtcagggtt ctgtctgtca
     4081 gaaccctgac atccgagccc ccccgaacct gccgggtgtc agaaccccgt ctatcagaat
     4141 cctgacgggt ggttcgaccg aaggcgaccc tctagccctg agtggtggct ccgacggaac
     4201 ccgaccctcc gaccgaaggc gacatcctag gagcgccgat ctgcgaagaa cgcccgcaac
     4261 acctcgccgc actcatccgc caggacgccc acggtcacgt cgacctcgtg gttgaggcga
     4321 ccgtcccgga cgagattgcc gagcgacccc gcgaaccccg ccttcggatc gctggcgccg
     4381 aacaccagcc gatcgagccg tgccagcacg atcgcgcccg cgcacatcgc gcacggctcg
     4441 agcgtgacga ccatcgcgca cccttcgagg tgccagctcc cgatcgcgcc cgacgcccgc
     4501 cggagcgcca ggatctcggc gtgggctgtg gggtccagtt ctcgttcgcg agcgttgccc
     4561 gcccgcgcca ggatctcgtc gccccgaacg atcaccgcgc ccaccggcac gtcgcccccc
     4621 gcggccgccg cccgcgccgc ctcgagcgcg agccgcatga tccgctcgtc gggggcatcc
     4681 accggtcagt agcggtcgat ctcgcgcagc cggtcgtcgc tgatcccgaa gaagtgggcc
     4741 acctcgtgct cgaccgtgtg ggcgatcacc tggcgtaggt gctcctcgtc gcgcgccacc
     4801 cgctcgatcg tccgctgata cagcgtgatc gtgtcgggga aggtgggccc cgattggaac
     4861 tggccgcgcc tggtcagcgg gaccccatgg tagcgcccga ggaggttcgg ctcgcccctg
     4921 ggcggacgat cctccaggat cagctcgatg ttctcgaggc gttcgcgcac ccagggcggc
     4981 aacgagtcga ccgcctccat gacgagatcc tcgaaccgct cccgatcgac cacgatcacc
     5041 cgcgccgtgc ggcgagcgac gacagcgcct ccatgaccac acgataggcg agcatcgacg
     5101 tgacctcgct ctggtcgtag ggcggcgaga cctcgaccac gtccatcccg gccagctcca
     5161 cggcggccac gatcctgcgt accgcgcgca gcagctcgcg ccc
//
LOCUS       DATBRK010000012         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_579316, whole genome shotgun sequence.
ACCESSION   DATBRK010000012 DATBRK010000000
VERSION     DATBRK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579316"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VIH70_00315"
     CDS             complement(<1..947)
                     /locus_tag="VIH70_00315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8114835.1"
                     /translation="MSDIVVVGGGIIGAACAFELADRGASVTLLEKDELAAGASGRNL
                     GYLDTSKDPVLAPLARRSLERYAEISADPPTPFFLDDEPIGTLAVTIDEDEIEDLRAW
                     VGSAEAVGVRVERLDERVGELEPELSTEVREAYLLHEGHRVDPLALTVCLGAVARTAG
                     ATIRHHAPARRLVAEGDRVAGVVTDDGVLAADAVFLAAGPWSPALARPLGVSLPIASA
                     RGWLVHAAPERPLFHHWIQSGARRLLAGRDLVPASEPHSVGLSMREFGEAAEARDVSP
                     MIQPAPDGSVLAGTSREPSFAADPFDLDVPRIVAAQASRL"
     gene            991..1079
                     /locus_tag="VIH70_00320"
     tRNA            991..1079
                     /locus_tag="VIH70_00320"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1025..1027,aa:Ser,seq:gct)
     gene            1126..1758
                     /locus_tag="VIH70_00325"
     CDS             1126..1758
                     /locus_tag="VIH70_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114836.1"
                     /translation="MWDWSTAASLATALGTLVLALATFVAVRAANRAARTAEYSMQIG
                     IRPLLMPSRLTDTEQKIMWGDEHWARLSGSGAVAEVADGYIYLAMSVRNSGSGVAVIH
                     GWHLGLQELTRDHPHAEPDEFRPQLRDLYVPGDDVGFWQAAIRNRDDPEYADLLEAVR
                     APRVFAIELLYSDHEGGQRAIGRFAVAPLKESVWLCSVVRHWNLDRPDPR"
     gene            2073..2145
                     /locus_tag="VIH70_00330"
     tRNA            2073..2145
                     /locus_tag="VIH70_00330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2106..2108,aa:Arg,seq:acg)
     gene            complement(2268..>2480)
                     /locus_tag="VIH70_00335"
     CDS             complement(2268..>2480)
                     /locus_tag="VIH70_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114837.1"
                     /translation="DRRKRGRPRRRLLHGVMEDAMRSVITWALDCGTKIICGPIAVIR
                     SQVAQYCRLQFFDAVVELPLRERSSR"
BASE COUNT          383 a          858 c          869 g          370 t
ORIGIN      
        1 agccgcgacg cctgggccgc cacgatcctc gggacgtcca ggtcgaacgg atcggccgcg
       61 aacgagggct cgcgtgacgt gccggcgagg acggatccgt cgggcgccgg ctggatcatg
      121 ggggagacgt cgcgcgcctc cgccgcctca ccgaactcgc gcatcgacag cccgacggaa
      181 tggggctcgc tcgcgggcac gaggtcccgg ccggccagca ggcggcgagc cccgctctgg
      241 atccagtggt ggaacagcgg acgctcgggc gccgcgtgga ccaaccaccc gcgggccgag
      301 gcgatcggga gcgagacgcc gagcggcctc gcgagcgccg gcgaccacgg cccggcggcg
      361 aggaagaccg cgtcggcggc gaggacgccg tcgtcggtca cgaccccggc gacacggtcg
      421 ccctccgcga cgagccggcg tgccggcgcg tggtggcgga tcgtcgcgcc cgccgtccgg
      481 gcgacggcgc cgaggcacac cgtgagtgcg agcgggtcca cgcggtgacc ctcgtgcagc
      541 aggtaggcct cgcgcacctc ggtcgagagc tcgggttcga gctcgccgac gcgctcgtcc
      601 agccgttcga cccgaacgcc caccgcctcg gccgagccga cccaggcgcg caggtcctcg
      661 atctcgtcct cgtcgatcgt gacggcgagc gtaccgatcg gctcgtcgtc gaggaagaac
      721 ggcgtcggcg gatcggccga gatctcggcg tagcgctcga ggctccggcg cgcgagcggc
      781 gcgaggaccg ggtccttcga ggtgtcgagg tacccgaggt tgcgcccgga ggcgccggcc
      841 gcgagctcgt ccttctcgag cagcgtgacc gatgcgcctc ggtcggcgag ctcgaacgcg
      901 cacgccgccc cgatgatccc gccgcccacc acgacgatgt cggacacggt cgcgagcgta
      961 ccggttgagc ccttccgtag aatccttccc ggagacgtgg ctgagtggcc gaaagcagcc
     1021 gcctgctaag cggttagggg ggtaacactc cctcgcaggt tcgaatcctg ccgtctccgc
     1081 cgagttcgaa ccacggtggc gaagccgttc gataggttgt cacgtatgtg ggactggtcg
     1141 acggcagcct cgctggcgac ggcgctgggg acgctcgtcc tggccctcgc gacgttcgtg
     1201 gcggtacgtg ccgccaaccg cgcggcgcgc accgctgagt actcgatgca gatcgggatc
     1261 cggccgctgc tgatgccgtc gcggctcacc gacaccgaac agaagatcat gtggggcgac
     1321 gagcactggg cccgcctctc gggctcgggc gcggtcgccg aggtcgccga cggctacatc
     1381 tacctcgcga tgtcggtccg taactccggt tcgggcgtcg cggtgatcca cggatggcac
     1441 ctggggctac aggaactgac ccgggatcac ccgcatgccg agcccgacga gttccgcccg
     1501 cagcttcggg acctctacgt ccccggcgac gacgtcggat tctggcaggc cgcgatccgc
     1561 aatcgcgacg atcccgaata cgccgacctg ctcgaggccg tccgggcgcc ccgcgtcttc
     1621 gcgatcgagc tgctctacag cgatcacgag ggtggccagc gcgcgatcgg gaggttcgcg
     1681 gtggcgccgt tgaaggagtc ggtgtggctc tgctccgtgg tccggcactg gaacctggac
     1741 cgccccgatc cccgctgacg gggctcgcgt gtcggctcag cgatcgacgt ctagcgacgc
     1801 ctacggaaca ggcgcgacca cagggacggt cgcgggtggc tccgcaagac ctcgtccccc
     1861 tgactgcgtt cagcggcgat ccggtcgatg tcgtgctctg tgtgttcggg tggcggaccc
     1921 gccggtcccg cggtcgaccc gtgacggaag tccgggtagt ccggactcgt gccccttcgt
     1981 tctgggctca tgcaacgacc ctacacacgc ccatcaaccc cccggaggtc ggcctcatgg
     2041 agcgctggac cccgccggta tgctggtacg gagcgccggt agctcaatgg atagagcatc
     2101 tgactacgga tcagaaggtt gggggttcga ctccctcccg gcgcgctagc agggaaaacg
     2161 catcgcaccc ttgacgcaca gcgctagcag agcgtataac gtcgtgctat ggaacttccg
     2221 cccgaacgcg agcagctcat cgtgttcgct cgcgagatcg gcgagctcta tcggctcgaa
     2281 cgttcccgca acgggagctc gacgaccgca tcgaagaact gaagacggca atactgcgcc
     2341 acctggctgc ggatcacggc gatgggtccg cagatgatct tcgtgccgca atcaagggcc
     2401 caggtaatga ctgatctcat ggcgtcttcc atgacgccat gaagcaggcg tcgacgcgga
     2461 cggccgcgct ttcgtcggtc
//
LOCUS       DATBRK010000013         4375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2023587, whole genome shotgun sequence.
ACCESSION   DATBRK010000013 DATBRK010000000
VERSION     DATBRK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023587"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VIH70_00340"
     CDS             complement(<1..1053)
                     /locus_tag="VIH70_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114838.1"
                     /translation="MNDLERDLREVLDEDARRAATPVSAPKGLRRSVRRRQAVFVGLM
                     GLACAAVVAGIVAGATTLLSLQSGPNPAAQGPTTTGTLNGITITYPQAWSLIDPDEAG
                     LNGSSMMAEVQLPRIILALAPTDPGKTFGCPGLAGGAATFLMTVQEEPLALDGPSSGP
                     WPVDLGAMTVDASESACYPNWDFLRAAWTAEGRTFEARVGFAPEASDEEHDAMAAAFA
                     SMTFEPSTDGVTSVALAHGTAGGEDWQLFASRDTGGLSLDISGETTGAGTAGLGTSST
                     NIQASTILFGSGSDAELVVFGAVPEGAVRVDASSADGSVTSTDVLDVPNEIDPGLNAF
                     VLVVAPGPLKLLGYDAS"
     gene            complement(1050..1541)
                     /locus_tag="VIH70_00345"
     CDS             complement(1050..1541)
                     /locus_tag="VIH70_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8114839.1"
                     /translation="MDDRADLDDLYVRDMPGAVRLAALITGDADLAQDIAHDAFLRSA
                     GRFRHLRQPEAFEAYLRRAVVNACTSHFRRRKVEAAYLRGQARRPASIDEPDHGRRDE
                     LRTALSALPPRQRAAVVLRYYADLSEQQASEALGCSPAAVRSMVARAMETLRERIPRS
                     EDT"
     gene            complement(1655..3562)
                     /locus_tag="VIH70_00350"
     CDS             complement(1655..3562)
                     /locus_tag="VIH70_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114840.1"
                     /translation="MNDLEHDLRELFDTRARDASVSPTPSHAIVRRARRRQVATVATS
                     VVLGGALVIASIAGISALTSSKREPRPLGVPEGEPGELSVVLPHATLSYPSDWYLLDL
                     GGQGAPRLQLSNFDPGLEPIDCDGDPSKVPSGGVVLSVAAGSVEWVDQPMRWPVDLEG
                     IADSGCEAAHAAWQTPEGDDLIATASLAPDASAPDRDALADAFASLVTSNEPMSEDLF
                     GDANLILDSIQTPPGPIAVYAYRADGSSWIGIAGPAGSRLSGAGQVGRDVPLADENVT
                     MILDTWGGVVWGDVSGAATRAALSTVEGRTYPAKLLPLPSSLGTDHQAVFGVIEGSTA
                     DRVTTLLYDAEGNVLNDFFPTGARVTIATGTDPEGGPWDLYLDTTNDGIGLGFGFTNH
                     GGGSGCCLRPLKEVFQLDGWGSGGDEPSNITALASEAVRRIVFEAATGERIEGGLYPV
                     PDASLGIPQVGLVIVPSDVTLEGQLVAYGASGDELAREDVGDNPEPAGPTTEIDIVWD
                     RLRRARTTVQDYGAQHHGSLAGFDALEATKIDPGIGGDPGIRWNDGQLADGEVSVRGA
                     EPAGGTAEMGLSGWTAVIVSATVAADGSPESIYCIAINIDENGGGNFRYGTQDAATYE
                     DCRGGWPELGP"
     gene            complement(3559..4071)
                     /locus_tag="VIH70_00355"
     CDS             complement(3559..4071)
                     /locus_tag="VIH70_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8114841.1"
                     /translation="MTEARTIEGTGGQIADLYERHVPVAGRFAYLLTGDRVLAQDLVQ
                     EAFVRVIGRFGHLRVPDAFEAYLRRTIVNLHTSSLRRRRLERDYLRRAQADKEPSTGL
                     PDVGAREGLWRALEQLTPRQRAAIVVRFYEDLSERETATLLRCSPAAVKSLVARAMDT
                     LRRWIEGEPR"
     gene            4151..>4375
                     /locus_tag="VIH70_00360"
     CDS             4151..>4375
                     /locus_tag="VIH70_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114842.1"
                     /translation="MPARDPDASKDPNMAEVKLVAEPREGTGKGVARKLRAAGRVPGV
                     LYGHGMDSTPLSVDAKDLFHVLHTGAGTNVL"
BASE COUNT          650 a         1539 c         1519 g          667 t
ORIGIN      
        1 ggacgcgtcg tagccgagga gcttgagagg accgggtgcg acgacgagca cgaaggcgtt
       61 caggccgggg tcgatctcgt tcggcacgtc gaggacgtcc gtggacgtga ccgacccgtc
      121 ggccgaggag gcatccaccc ggaccgctcc ttcggggacg gcgccgaaga ccacgagctc
      181 ggcgtccgag ccggagccga agaggatggt ggacgcctgg atgttcgtcg aggacgtacc
      241 gagccccgcc gtgcccgccc ccgtcgtctc cccggagatg tcgaggctga ggccgcccgt
      301 atcgcgggag gcgaacagct gccagtcctc cccgccggcg gtcccgtgtg ccagggcgac
      361 cgaggtgacg ccgtcggtcg acggctcgaa cgtcatcgag gcgaacgccg cggccatcgc
      421 gtcgtgctcc tcgtcgctcg cctccggagc gaacccgacc cgggcctcga acgtccggcc
      481 ttccgccgtc cacgcggcgc ggaggaagtc ccagttcggg tagcacgccg actcggacgc
      541 gtccacggtc atcgccccga gatcgaccgg ccacggtccg gacgagggcc cgtcgagcgc
      601 caacggctcc tcctggaccg tcatgaggaa ggtcgctgcg ccaccagcca ggcccgggca
      661 gccgaacgtc tttccgggat cggtcggcgc cagcgcgagg atgatgcgcg gcagctggac
      721 ctccgccatc atgctggacc cgttcagccc cgcttcgtcc gggtcgatca ggctccacgc
      781 ctgcgggtag gtgatcgtga tcccgttgag ggtgccggtc gtcgtcggcc cctgggcggc
      841 ggggttcggg ccgctctgga gggacagcag cgtcgtcgcg ccggcgacga tcccggcgac
      901 aaccgcggcg caagccagtc ccatcaggcc cacgaacacc gcctgccggc gacggacgga
      961 tcgccggagc cccttggggg ccgagaccgg ggtcgcggcg cgccgggcgt cctcgtcgag
     1021 cacctcacgc aggtcgcgtt cgagatcgtt cacgtgtcct cactcctcgg gatccgttcg
     1081 cggagcgtct ccatcgcgcg ggcgaccatc gaccgcacgg ctgcaggcga gcatccgagc
     1141 gcctcgctcg cctgctgttc ggacaggtcg gcgtagtagc ggagcacgac ggccgcccgc
     1201 tgccgcggtg ggagcgccga gagcgcggtc cggagctcgt cgcggcggcc gtggtcgggc
     1261 tcgtcgatcg acgccggacg gcgcgcctgg ccgcgcaggt acgccgcctc caccttccgg
     1321 cggcggaagt gcgacgtgca cgcgttcacc accgcccgtc gcaggtaggc ctcaaacgcc
     1381 tcgggctgcc ggagatgacg gaacctgccg gccgaccgca ggaacgcgtc gtgggcgatg
     1441 tcctgggcaa ggtccgcgtc gcccgtgatc agggcggcca gccgaacggc ccccggcatg
     1501 tcccgcacgt agaggtcgtc gaggtcggcg cgatcatcca cggcggaacc gacgataccg
     1561 gtggtctggc tcatccctct tcagacgcgg gagtccgagg tctcatagcg gtatccgtgg
     1621 ctggttctcc gccgatattc gagagaagct ctggctacgg gccgagctcg ggccaccctc
     1681 cccggcaatc ctcgtaggtc gccgcgtcct gcgtcccgta ccggaagttg ccgcccccgt
     1741 tctcgtcgat gttgatcgcg atgcagtaga tcgactcggg cgatccgtcc gcggcaaccg
     1801 tcgcggacac gatgactgcg gtccagccag acaggcccat ctcggccgtg ccgcccgccg
     1861 gttccgctcc ccggacgctc acttctccat ccgcgagctg accgtcgttc cagcggatcc
     1921 cgggatcgcc tccgatgccg gggtcgatct tggtggcctc gagtgcgtcg aagcccgcga
     1981 gcgacccatg gtgttgagct ccatagtcct ggaccgtcgt gcgggcccgg cggagcctat
     2041 cccagacgat gtcgatctcc gtcgtcgggc cggcgggctc cgggttgtcg cccacgtcct
     2101 ctcgtgcgag ttcgtcaccg ctcgcgccgt acgcgacgag ctggccctcg agggttacgt
     2161 ccgacgggac gatgacaagg cccacctgtg ggatccccag cgaggcatcc ggcaccgggt
     2221 acagccctcc ttcgatgcgc tcgcctgtcg cagcttcgaa gacgatccgc ctgacggcct
     2281 ccgaggcgag cgccgtgatg ttgctcggtt cgtcgcctcc actgccccag ccgtcgagct
     2341 ggaaaacctc cttcagcggc cgcaggcagc aaccgcttcc gccgccgtga ttcgtgaagc
     2401 cgaagccgag cccgatcccg tcgttcgttg tgtcgaggta gaggtcccac ggcccgccct
     2461 cgggatcggt gccggtcgcg atcgtgacgc gtgcgccggt cgggaagaag tcgttgagga
     2521 cgttgccctc ggcgtcgtag agcaacgtgg tgacgcgatc ggccgtcgat ccctcgatga
     2581 ccccaaacac cgcctggtga tcggtgccga gactcgaggg gagcggcagc aactttgcgg
     2641 gataggttcg gccctcgacc gtactgagcg cggctctggt tgccgcgccc gagacgtcgc
     2701 cccagacgac gccgccccag gtgtcgagga tcatggtgac gttctcgtcg gccaggggga
     2761 cgtcgcggcc gacctggccc gcgcccgaga ggcggctacc tgccggtccg gcgatcccga
     2821 tccaggacga tccatccgcc cgatacgcgt agaccgcgat cggcccaggt ggcgtctgta
     2881 ttgaatcaag gatcaggttg gcatcgccga ataggtcttc ggacatcggc tcgttcgacg
     2941 tgaccaggct tgcgaacgcg tcggcgaggg cgtcgcgatc gggcgcgctg gcatcgggag
     3001 cgagggatgc cgtcgcgatg aggtcgtccc cctccggggt ctgccaagcc gcgtgagcag
     3061 cttcgcatcc gctgtcggcg atgccctcca ggtccaccgg ccatcgcatg ggttgatcga
     3121 cccactcgac ggagcccgcg gccaccgaca acacgacgcc accggacggg accttggaag
     3181 gatccccgtc gcaatcgatg ggctcgaggc ccggatcgaa gttcgacagc tggagccgcg
     3241 gcgcaccttg gcctccgagg tcgagcaggt accaatcgga cggatagctc aatgtcgcgt
     3301 gcgggagcac cacggacagc tcgcccggct ccccttcggg cacccccagc ggcctgggtt
     3361 cgcgcttcga cgaggtgagc gccgagatcc cggcgatcga cgcgatcacc agtgcgccac
     3421 caaggacgac cgatgttgcg acggtcgcga cctggcgacg acgggcccgc ctcacaatgg
     3481 cgtgcgaggg cgttggcgag accgacgcgt ctcgggccct cgtgtcgaag agctctcgaa
     3541 ggtcgtgttc gaggtcgttc atctcggctc gccttcgatc catctcctga gcgtgtccat
     3601 cgctcgggcg accaacgact tcacggcggc gggggagcag cgcaggagcg tcgccgtctc
     3661 gcgctcggac aggtcttcgt agaagcgcac gacgatcgcg gctcgttggc gcggtgtgag
     3721 ctgctcgagc gcccgccaga ggccctcgcg tgcgccgaca tccggcaggc ccgtcgacgg
     3781 ctccttgtcg gcctgcgctc ggcgcaggta gtcacgttcg aggcgtcgtc gccgcaggga
     3841 ggacgtgtgc aggttcacga tcgtcctgcg caggtacgcc tcgaaggcgt cggggacgcg
     3901 cagatggccg aaccgaccga tgacacggac gaaggcctcc tgcacgaggt cctgggccag
     3961 gacgcgatcg ccggtcagga ggtaggcgaa cctcccggcg accggcacgt gacgttcgta
     4021 caggtcggcg atctggccgc cggtaccctc gatggtgcgg gcttcggtca cgaggatccc
     4081 cacactatgg aagacgctcg gcgcgcgcgg tcggtgtcgc cccgacgcat ccccagagcg
     4141 tattggtacc atgcccgccc gagaccccga cgcctcgaag gacccgaaca tggccgaagt
     4201 caagctcgtc gccgagccca gggaaggcac cggtaagggc gtcgcccgca agctgcgggc
     4261 cgccggccgc gtgccggggg tgctctacgg gcacgggatg gactcgaccc cgctgtccgt
     4321 ggacgccaag gacctgttcc acgtcctcca taccggcgcc ggcaccaacg tcctg
//
LOCUS       DATBRK010000014         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1157170, whole genome shotgun sequence.
ACCESSION   DATBRK010000014 DATBRK010000000
VERSION     DATBRK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157170"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VIH70_00365"
     CDS             complement(<1..426)
                     /locus_tag="VIH70_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8114843.1"
                     /translation="MARAAVRGRLNWTLGEVVELIDETPRVRTIFLEVPEWPGHRAGQ
                     HVDVRLTAEDGYQAQRSYSIASSPGDGSLAITVERLPDGEVSEYLVGELRRGDMLELR
                     GPIGGYFVWEARSDGPLLLVAGGSGIVPLMSMLRYRQASD"
     gene            complement(396..1001)
                     /locus_tag="VIH70_00370"
     CDS             complement(396..1001)
                     /locus_tag="VIH70_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEY8114844.1"
                     /translation="MGYVSRGFRGKRASEREDTRLPPGQYLVDDFPVLSAGPTPHTPL
                     DEWTFAIQNEDAILKTWSWGQFRALPSEDIRADISCVTKWTKLDTTWEGVAIDTLLEG
                     VQHQPTHLMAFSDGDYTTNLPLEDVLDGQAWVAFAYDGRPLEAEHGGPARLLVPHLYF
                     WKSAKWVRGLRLMDHDEPGFWESYGYHIYGDPWREQRYAGD"
     gene            1238..1729
                     /locus_tag="VIH70_00375"
     CDS             1238..1729
                     /locus_tag="VIH70_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8114845.1"
                     /translation="MSPDEADFRRVYEQAFPIVYRVAYRFAGRREAAEDAAQEAFARA
                     FERWGRLRAEPWVVGWVVTTALNILRRGARRAPDPSREGEEPRSPATDVEGALDLWRA
                     IRELPRRQAQAVVLYYIVDLPVAQVAEVMGCREGTAKAHLDRARDRLARLLEPSEARR
                     EEG"
     gene            1732..>1985
                     /locus_tag="VIH70_00380"
     CDS             1732..>1985
                     /locus_tag="VIH70_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114846.1"
                     /translation="MRVDEEIRSRLERSLRTVEAPSTRVEPVVRRARSLRRRHRAAAM
                     TVAAVGIVALVVPLVLLWPLGPDEVADVGPATEPGRLVVSA"
BASE COUNT          281 a          668 c          726 g          310 t
ORIGIN      
        1 gtccgacgcc tggcggtatc gaagcatgga catcagcggg acgatgccgg agcctccggc
       61 cacgagcagg agcggcccgt ccgagcgggc ctcccacacg aagtagccgc cgatcggacc
      121 gcggagctcg agcatgtcgc cacgccgcag ctcgccgacg aggtactccg agacctcacc
      181 gtcgggcagg cgctccaccg tgatcgcgag ggacccgtct cccggagacg aagcgatcga
      241 gtagctgcgc tgcgcctggt agccatcctc ggcggtgagg cgaacgtcga cgtgctggcc
      301 ggcccggtgg ccgggccact cggggacctc caggaagatg gtccggaccc gaggcgtctc
      361 gtcgatcagc tcgacgacct cgccgagcgt ccagttcagt cgcccgcgta ccgctgctcg
      421 cgccatgggt ctccgtagat gtgatagccg tagctctccc agaagcctgg ctcgtcgtga
      481 tccatcagcc gaagaccgcg aacccacttc gcgctcttcc agaagtacag gtggggaacg
      541 aggagccggg cgggcccccc gtgttcggcc tcgagcggcc gtccgtcgta ggcgaacgcg
      601 acccacgcct gaccgtcgag gacgtcctcg agcggcaggt tcgtcgtgta gtcgccgtcc
      661 gagaacgcca tcaggtgggt cggctggtgc tggacgccct ccagaagcgt gtcgatcgca
      721 acgccctccc aggtggtgtc cagtttcgtc cacttcgtga cgcagctgat gtcggcccgg
      781 atgtcctcgc tgggaagagc ccggaactga ccccagctcc aggtcttgag gatcgcatcc
      841 tcgttctgga tggcgaacgt ccactcgtcg agcggcgtgt gcggcgtggg acccgccgac
      901 agaaccggga agtcgtcgac gaggtactgg cccgggggga gccgcgtgtc ctcacgctca
      961 ctcgcgcgtt tgccgcggaa gccgcgggag acgtacccca tccgttcctc cgtcctatcg
     1021 tccctcgagc cgtcgtgatc gtatgccgct tcggcctcgg gagcatccgc cacgaggtcg
     1081 gcgtcgtcga acccggtcag gcgtagtcgc ccggccgggg cacggggatc acggccggtt
     1141 cgctaggccg cgatcccccg ggctcggcgt cgatcctggc cggcgaggta aacctcacac
     1201 gtcgctccgg gcttatcgga tggaggatcg ttgatgggtg tctcctgacg aggcggactt
     1261 ccgccgggtg tacgagcagg cgttcccgat cgtgtatcgc gtagcgtatc gcttcgccgg
     1321 gcggcgcgag gcggccgagg acgccgcgca ggaagccttc gcgagggcgt tcgagcgctg
     1381 gggccgcctg cgcgcagagc cgtgggtcgt cggatgggtc gtcacgaccg ccctgaacat
     1441 ccttcgcagg ggggcgcgcc gggcgcccga tccgtcgcgg gagggtgagg agcctcgctc
     1501 cccggcgacg gacgtggagg gcgcgttgga cctgtggcgt gcgatcaggg agctccccag
     1561 gcggcaggcg caggcggtgg ttctgtacta catcgtcgac ctgccggtcg cgcaggtcgc
     1621 ggaggtcatg ggctgccggg aggggaccgc caaggcgcac ctcgaccgag cccgtgaccg
     1681 gctggccagg ctgctggagc cgtcggaggc tcgacgggag gagggatgac gatgcgcgtc
     1741 gacgaggaga tcaggtctcg gctggagcgg tcgctcagga ccgtcgaggc gccttcgacc
     1801 cgggtcgaac cggtcgtccg ccgcgcgagg agcctgcggc gccgacatcg tgccgccgca
     1861 atgaccgtcg ccgccgtcgg gatcgtggcc ctggtcgttc cgctggtgct gctgtggccg
     1921 ctcggtccag acgaggttgc cgacgtcggc cccgcgaccg agccgggccg gctggtcgtg
     1981 tcggc
//
LOCUS       DATBRK010000015         3955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127 k141_1244,
            whole genome shotgun sequence.
ACCESSION   DATBRK010000015 DATBRK010000000
VERSION     DATBRK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VIH70_00385"
     CDS             complement(<1..542)
                     /locus_tag="VIH70_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114847.1"
                     /translation="MRVLFLTPRQLGEPRSGGTIKSAAVLSYLEARHEVEVVCFRGHD
                     EQPWMREGGVVSVPLERDRSVRHLLSSYAAGVPLSIERNRSSTMAERVAALLEPGDHG
                     AIFVDGWLMAQYVPEEFVGIRLLHEHNAEHVMWLRHAELETNPTRRPLVRLEARRVRK
                     YEAGLLARFDVVFAVSERDRA"
     gene            complement(543..1295)
                     /locus_tag="VIH70_00390"
     CDS             complement(543..1295)
                     /locus_tag="VIH70_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY8114848.1"
                     /translation="MTSVIFGVRLDELASAEQLRGVATGFLDGDRAFRIFTPNPEILL
                     RARDDPAYADLLRSADLALPDGTGVAIVQAMWSGRSVRRWPGVEIAAMVLRLAAERGE
                     TVAFVGGSTDVAERAAARWRALPGLKVVVAGAGVAIDEDGIARSMAREDETVEAIRSA
                     APTIVLVGLGAPKQERWIERHADAFPSVRIMIGVGGAFDMWAGSKRRAPRAFRTFGLE
                     WLWRLALEPRRLPRIVRATVVFPVLALFDRAG"
     gene            complement(1292..2011)
                     /locus_tag="VIH70_00395"
     CDS             complement(1292..2011)
                     /locus_tag="VIH70_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114849.1"
                     /translation="MDRIKYSVMTRLAPYPSLALPLARLRGEGELVDSRTDLVVESYP
                     RCASSFAIAAFRLAQEPRSLRVAHHTHASGHVIAGIRLGIPTLVLIREPQGAVVSSRI
                     RHPARTYADLLRGFCAFYEPLVPHRGDLVVGTFDEVVGGELGAITRRLNARFGTRFAE
                     FEPTEENVARCMREIDEDWRIRRGDGDRLERVVPRPSPVREGLKAELHERYAAEAPPN
                     LRHRAERLFSWFTRTEDPEAS"
     gene            2081..3217
                     /locus_tag="VIH70_00400"
     CDS             2081..3217
                     /locus_tag="VIH70_00400"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY8114850.1"
                     /translation="MRIAIDARPASDAEPTGVGHYTRRMLSYLPAAMPADELVAWHPQ
                     TGGSDLPRAPNLTEHVSRIPGVLEPVWRRLELPRLEGMVRFDALLATNFLPPPTRSDA
                     VVLVVHDLAFEVMPEAGPGFGERWRRRFDGWLRRGAGVIVPSASARADLLRRHTVDEG
                     RVRVIPHGADPFEAPAPEEVERVLARFRIGGPFALFVGGIEPRKNLGALIEAFAHVGD
                     ADRWLVIAGGRTYWAPQGTETLRAQVAALPEDVRRRVVLTGYVSDEEKRALLAGATVL
                     TYPSRYEGFGFPVLEGFSAGVPVLTSSAASLLEVAGDAALLVDPNDVDAIAEGLVRLF
                     GDAGLRERLIAAGRERLATFSWQRAADATAEVLRGAAGRSAGAG"
     gene            3231..>3955
                     /locus_tag="VIH70_00405"
     CDS             3231..>3955
                     /locus_tag="VIH70_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8114851.1"
                     /translation="MRIPLHRRALPWVAAVLGLAMLAAACSASEAEPRATSSPPASPS
                     PSVSTTGSPEAFDATALETTTPIKHVVFLIKENRTFDNLFGTFPGANGVSTGMAFGRP
                     RTLTRGTDGRVPGDLPHCYTCALAAWDQGEMDGFLQGDHAPWAYTQLHKDQLPNYWHW
                     ARQNVLFDDFFASAQGPSFPNHLYSIAATSGGAHDNPRRTPSLTHGSNTFGCDAPRLQ
                     TVEVYDSEGRVKKVPPCFDFLTEG"
BASE COUNT          559 a         1432 c         1439 g          525 t
ORIGIN      
        1 gcccggtcgc gttcggagac cgcgaacacc acgtcgaacc gcgcgagcag acccgcctcg
       61 tacttccgga cccgccgggc ctccagccgg accaggggcc tgcgagtcgg attggtctcg
      121 agctcggcgt gacggagcca catcacgtgc tcggcgttgt gctcgtgcag caggcggatc
      181 cccacgaact cctcgggcac gtactgcgcc atcagccagc cgtcgacgaa gatggcgccg
      241 tgatcgccgg gctcgaggag cgcggcgacg cgctcggcca tcgtcgagct gcggttgcgt
      301 tcgatgctga gcggcacgcc ggcggcgtag ctcgagagga ggtggcgaac cgatcggtcc
      361 cgctccaggg ggaccgagac aacgccgcct tcacgcatcc agggctgttc gtcgtgaccg
      421 cggaagcaga cgacctcgac ctcgtgccgg gcctccaggt acgacaggac cgccgccgac
      481 ttgatcgtcc ctccgctgcg gggctcgccc agctggcgcg gcgtcaggaa caggacccgc
      541 actcagccgg cccgatcgaa cagggcgaga accgggaaga ccacggtcgc ccgcacgatc
      601 cggggaagcc gtcggggctc gagcgccagc cgccacagcc attcgagccc gaacgttcgg
      661 aacgcgcggg gcgcgcgtcg cttggagccc gcccacatgt cgaacgcgcc gccgaccccg
      721 atcatgatgc ggaccgacgg gaacgcgtcc gcgtggcgct cgatccaccg ctcctgcttg
      781 ggcgcgccga gccccacgag aacgatcgtg ggcgcggccg aacggatcgc ctcgacggtc
      841 tcgtcctcgc gagccatcga gcgggcgatg ccgtcctcgt cgatcgcgac gcccgcgccg
      901 gcaaccacga ccttcagccc cgggagggcg cgccatcgag ccgccgcgcg ttcggcgacg
      961 tcggttgagc cgccgacgaa cgccacggtc tcgccgcgct cggcggcaag gcgcagcacc
     1021 atcgcggcga tctccacccc gggccagcgt cggaccgagc ggccgctcca catcgcctgc
     1081 acgatcgcga cgcccgtccc gtcggggagc gcgaggtcgg ccgatcgaag gaggtccgcg
     1141 taggcaggat cgtcgcgcgc gcgcaggagg atctcggggt tcggcgtgaa gatccggaac
     1201 gcgcggtcgc cgtcgaggaa cccggtcgcg acgccgcgca gctgctcggc cgacgcgagc
     1261 tcgtccaggc ggacgccgaa gatgaccgac gtcacgacgc ctccgggtcc tcggtccgcg
     1321 tgaaccacga gaacagccgc tcggcgcggt gacggaggtt cggcggcgcc tcggcggcgt
     1381 agcgctcgtg cagctcggcc ttcaacccct cgcgcaccgg cgagggtcgg gggaccacgc
     1441 gttcgaggcg gtcgccgtcg ccgcgtcgga tgcgccagtc ctcgtcgatc tctcgcatgc
     1501 agcgcgcgac gttctcctcg gtcggctcga actccgcgaa cctggtgccg aagcgcgcgt
     1561 tcagtcgccg cgtgatcgcg ccgagctcgc cgccgaccac ctcgtcgaac gtcccgacca
     1621 ccaggtcgcc gcgatggggc acgagcggct cgtagaaggc gcagaaccct cgaagcaggt
     1681 ccgcgtaggt ccgcgcggga tggcggatgc ggctgctcac caccgcgccc tggggctcgc
     1741 ggatcaacac gagcgtcggg atccccaacc ggatccccgc gatcacgtga cccgacgcgt
     1801 gcgtgtggtg ggcgacgcgg aggctcctcg gctcctgtgc gaggcggaat gccgcgatcg
     1861 cgaacgagga ggcgcagcgc gggtaggact cgacgacgag gtcagttcgc gagtcgacca
     1921 gttccccctc gccgcgtaac cgagccagcg gcagtgcgag cgaggggtac ggagcgagtc
     1981 gcgtcatgac cgaatacttg atccggtcca cgcccggatg ctaccgctcg ggcgttgggc
     2041 cggacccgtg cctcgcgcgg cgacgggcga tactgcccgc gtgcggatcg cgatcgacgc
     2101 tcgtcccgcc tccgatgccg aacccaccgg cgtgggccac tacacccgac ggatgctgtc
     2161 gtacctgccg gcggcgatgc cggccgacga gctggtggcg tggcacccgc agacgggcgg
     2221 ctccgacctc ccgcgcgccc cgaacctgac cgagcatgtg tcgaggatcc ccggggtcct
     2281 cgaacccgtg tggcggcgtc tcgagctgcc gaggctcgag gggatggtgc ggttcgacgc
     2341 cctcctcgcg acgaacttcc tcccgccgcc gacccgcagc gacgccgtcg tgctggtcgt
     2401 ccacgacctg gcgttcgagg tgatgcccga ggcgggtccc gggttcggcg agcggtggcg
     2461 gcggaggttc gacgggtggc tgcggcgcgg cgccggtgtg atcgtgccgt cggcgagcgc
     2521 gagggccgac ctgctccgac gccacacggt cgacgagggc cgcgtgcgcg tgatcccaca
     2581 cggggcggac cccttcgaag cgcccgcgcc ggaggaggtc gaacgcgtcc tcgcgcggtt
     2641 ccggatcggc gggccgttcg cgctgttcgt cggcggaatc gagccgcgga agaacctcgg
     2701 cgcgctcatc gaggcgttcg cgcacgtggg cgacgcggat cggtggctcg tgatcgctgg
     2761 cggtcgcacg tactgggcgc cccagggcac cgagacgctc cgcgcgcagg tcgccgcgct
     2821 ccccgaggac gtccgccgac gggtggtgct caccggctac gtgtcggatg aggagaagcg
     2881 tgcgctgctc gcgggcgcga cggtgctcac ctacccgtcg cggtacgagg ggttcgggtt
     2941 cccggtgctc gaggggttct cggccggcgt cccggtgctg acgtcgtccg cggcctcgct
     3001 gctcgaggtg gccggggatg ccgcgctcct ggtcgacccg aacgacgtcg acgcgatcgc
     3061 cgagggtctc gtccggctgt tcggcgacgc aggcctgcgc gagcggctta tcgccgccgg
     3121 ccgcgagcgt ctcgcgacgt tctcgtggca acgggcagcc gatgcgacgg cggaggtgct
     3181 ccggggggcc gccggccgtt cggccggcgc tggatagact ccgggcatcc atgaggatcc
     3241 ccctgcatcg acgcgcgttg ccctgggtcg ccgccgtcct gggcctggcg atgctcgccg
     3301 cggcgtgcag cgcgagcgag gccgagccgc gggcgacgag ctcgccgccc gcgtccccga
     3361 gcccgagcgt gagcaccacc gggagccccg aggcgttcga cgcgaccgcg ctcgagacga
     3421 cgacgccgat caagcacgtc gtgttcctga tcaaggagaa ccggacgttc gacaacctct
     3481 tcggcacgtt cccgggcgcg aacggggtct cgaccgggat ggcgttcggg cggccgcgga
     3541 cgctcacgcg cgggaccgac ggccgggtgc ccggcgacct gccgcactgc tacacgtgcg
     3601 cgctcgcggc gtgggaccag ggggagatgg acgggttcct gcagggcgac cacgccccgt
     3661 gggcctacac gcagctccac aaggaccagc tgccgaacta ctggcactgg gcgcggcaga
     3721 acgtcctgtt cgacgacttc ttcgcgagcg cgcagggccc ctcgttcccg aaccacctct
     3781 actcgatcgc cgccacctcg ggcggcgcgc acgacaaccc gcgacggacg ccgagcctca
     3841 cccacggctc gaacacgttc ggctgcgacg cgccgcggct gcagaccgtc gaggtctacg
     3901 acagcgaggg gcgggtgaag aaggtgcccc cgtgcttcga cttcctcacc gaggg
//
LOCUS       DATBRK010000016         7226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1879345, whole genome shotgun sequence.
ACCESSION   DATBRK010000016 DATBRK010000000
VERSION     DATBRK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879345"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VIH70_00410"
     CDS             <1..930
                     /locus_tag="VIH70_00410"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY8114852.1"
                     /translation="MKDALKPNLVQTLEGQPVLIHTGPFGNVAHANNSIIEDRVGLKL
                     ADYVITEAGFASDLGFQKFCDIVCRLGGFAPSAGVLVTTVRATKSHGGVPFDQLGTED
                     LEAVKRGVDNLGQHIAIVREYGLPCVVSINNFPSDTGAEVELMRELALGAGAETVVVN
                     RGFAQGGGGAVELAEAVAAACERPNAFHQLTPDDTPIKDQVEAIATRVYGADGVDYQA
                     QAEKDLVRMDRLGFGTLPVCMAKTHLSLTHDPSLLNRPRGWRLPVRGVVPSAGAGFVV
                     VLCGEMQRMPGLGRTPAYMQVDIDEHGRTIGLF"
     gene            complement(943..1335)
                     /locus_tag="VIH70_00415"
     CDS             complement(943..1335)
                     /locus_tag="VIH70_00415"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010039573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8114853.1"
                     /translation="MAVELRNVHALAPPPGYSHVAVGRGGTVVFTAGAVPLDAQGDLV
                     GGDDPVAQAEQVIENLLAALEAGGARPDNVAKTTVYVAGSSYEAQSAVWQVVRGSEIG
                     RAPSTLVGVPILGYRGQLVEVEAIALID"
     gene            complement(1337..1525)
                     /locus_tag="VIH70_00420"
     CDS             complement(1337..1525)
                     /locus_tag="VIH70_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114854.1"
                     /translation="MPLHTCAPPRDAQHTTMSLWVCPECGSTWEGVPWSGVFDFDRSE
                     AVTRAEWILVEGPTQLAG"
     gene            1680..2477
                     /locus_tag="VIH70_00425"
     CDS             1680..2477
                     /locus_tag="VIH70_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114855.1"
                     /translation="MSISSTLERPRRREALPSALRGFAAVFVAGVVAGVVVGGVGSRI
                     AMRIAALTGRDAAQGLTTEAGATIGRITFEGTAFLVVAVGIGSALVGTAFYLATLPWL
                     PRRRTPRALAFGYLELAVFGSVLLDPGNPDFTILGRPLLDVLVLGSLFVLHGVALVML
                     VEPSGRLVSAVARGGRWRARLVAVGTFVAMALTALGALALLARATGWAAPVTLVLLLC
                     AAGLLVLDPRPTRPVTRPALRVTGAVALTLLTISGTVGLVDAVTTIV"
     gene            complement(2481..3587)
                     /locus_tag="VIH70_00430"
     CDS             complement(2481..3587)
                     /locus_tag="VIH70_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY8114856.1"
                     /translation="MGDDDAIRTAFHDVTEAQGGTHIADGGWLWLEGFGDVVKEYAAI
                     RDDVAVWDVSPLNKWDLRGSDGLRAAQRLFSNDALGLDVGQARYGAFLDTDGLMVDDG
                     TVFNTGAGHCWVMTNGKDHDDYFGEALRDLDVEFEWIAPRMPHLGVIGPRSREVVRKL
                     TDADVDALRYFRFLPEPVKVARVEVYLSRTGYGGELGFELFLTDPSDAEELWNAVVGA
                     GATPFGAEAIELARIEAGLIVTDYDYEAHQRTPYDFNLDRFVRLDAPGEFLGKEKLRD
                     VAATPPNRLVTLRYEADALPEYGAAVTKGGEEIGVLTSPTETPRFGKIGIAIVPTVEA
                     VVGNTVQVAMGDGTVDATVDVLPIYDTNKDRPRS"
     gene            complement(3671..4255)
                     /locus_tag="VIH70_00435"
     CDS             complement(3671..4255)
                     /locus_tag="VIH70_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8114857.1"
                     /translation="MRSRPWREAEFASLDFETTGLDRERDAVVSFGVVPVREGRVVVG
                     EAVHQLVLPEVPSSAESMRIHGILPKDLAHAQPFPVAGQTLRLALAGRFLLAWFASVE
                     IGFLGRLYGGRRRSWVRRTIDVRQMAIELEHLDQDVRQSLSSAAEHYGVPVANPHEAL
                     DDAMVTAQLFLVLADRLEERGLGTARSLLRLTRV"
     gene            complement(4282..4869)
                     /locus_tag="VIH70_00440"
     CDS             complement(4282..4869)
                     /locus_tag="VIH70_00440"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY8114858.1"
                     /translation="MSDLPAHRLKQAKRALRRAVLAQRDAVPQDDRSERSEAIADRFL
                     GLAETAGADTVLAFWSFGSEVDTGPLIARLRSQGKTVALPRIEGNEVAPVVATPGSRM
                     REASFGAMEPAEGRALDVAELDLVVVPGVAFDRSCGRVGYGGGYYDRLLGKRPDGVAA
                     IAIAFALQIVDRVPSGAIDRRIDGIVTEVEVIRCR"
     gene            complement(4866..5765)
                     /locus_tag="VIH70_00445"
     CDS             complement(4866..5765)
                     /locus_tag="VIH70_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8114859.1"
                     /translation="MKVGLVLPMFSGDAGRVLAFARRAEERGFDGVFAFDHLFPPGAA
                     PDRPSLEAYATLAAVAAATSRIAVGTLVTRATLRPAGMLAKLAAAVDDVGGGRMILGI
                     GTGDPIDELEHRVFGIAYLGKAERRGHLVETVRAIKALYGGVRWDGGTYVPAMTGPLL
                     PPPVRPDGPPIWIGGFADAVVRLAAAEADAWNGWGMGVEEFTRKVQLLREAAGERPVS
                     ATWAGIVVVGRDHAEVGRLLEQRSARGLEPGVWSGTTASLRTHLRALADAGSTWSVLV
                     PGGPPDRLDVIAEEVLPELRSSA"
     gene            complement(5767..6735)
                     /locus_tag="VIH70_00450"
     CDS             complement(5767..6735)
                     /locus_tag="VIH70_00450"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEY8114860.1"
                     /translation="MPERGSGEAIGLDVGGTKINAFRVTRDGTIVERSSSPTPAEDEA
                     ATLSTMVELARSLTTPDVTAIGVGAAGLVDAKEGVMRFSPNLAWRNLPIAERMRDALG
                     LPCQVDNDASMAAYGEFRFGAGRGYRHLLLVTVGTGIGGGIVSDGRLFRGANGFAAEI
                     GHIIVEPGGPLCGCGNRGCWEQVAAGRAIDRMGREEAREHGHSILRRLAGGDPDEVTG
                     ELVTEAAREGDEAARGILSEAGRRLGQGIAGLVNVLDPQVVVVGGGAIVAGDLLLDPA
                     RAAYLDAVEAPEHRPRVPIVPAELGNDAGAVGAATLALEELGRVMV"
     gene            complement(6770..7060)
                     /locus_tag="VIH70_00455"
     CDS             complement(6770..7060)
                     /locus_tag="VIH70_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114861.1"
                     /translation="MPETEPGHAASEGDGAAGSASEAQPQPPVCTVAFCPICMAVTAG
                     QGAAPDVFEHLLAAAREFFLAARAVIDVRGDHVSGVGKSPNGSKDFERIEIS"
BASE COUNT          984 a         2679 c         2522 g         1041 t
ORIGIN      
        1 atgaaggacg cgctgaagcc caacctggtg cagacgctcg aggggcagcc cgtcctgatc
       61 cacaccggtc cgttcggcaa cgtcgcccac gcgaacaact cgatcatcga ggaccgcgtc
      121 gggctgaagc tcgccgacta cgtcatcacc gaggcgggct tcgcgagcga cctggggttc
      181 cagaagttct gcgacatcgt gtgccggttg ggggggttcg cgccctcggc cggtgtgctc
      241 gtgacgaccg tccgggccac gaagtcccac ggcggcgtgc cgttcgatca gctggggacc
      301 gaggacctgg aggcggtcaa gcgcggcgtg gacaacctcg gccagcacat cgcgatcgtc
      361 cgtgagtacg gcctgccgtg cgtcgtctcg atcaacaact tcccgagcga cacgggggcc
      421 gaggtcgagc tgatgcgcga gctcgcgctc ggggccggcg cggagacggt ggtggtgaac
      481 cgcgggttcg cccagggcgg gggcggcgcg gtcgagctcg cggaggcggt ggcggccgcg
      541 tgcgagaggc cgaacgcgtt ccatcagctg acgcccgacg acacgccgat caaggaccag
      601 gtcgaggcga tcgcgacccg cgtgtacggc gcggacggcg tcgactacca ggcccaggcg
      661 gagaaggacc tcgtgcggat ggaccggctc gggttcggca cgctgccggt ctgcatggcg
      721 aagacgcacc tgtccctcac ccacgacccg tcgctgctca accgcccccg agggtggcgt
      781 ctccccgttc gcggcgtggt gccgagcgcg ggcgcggggt tcgtcgtcgt gctctgcggc
      841 gaaatgcagc ggatgcccgg tctcggcagg acccccgcgt acatgcaggt agacatcgac
      901 gagcacggtc ggacgatcgg gttgttctga ggcggctgtc ggtcagtcga tgagcgcgat
      961 cgcctcgacc tcgacgagct gaccgcgata gccgaggatc gggacgccga cgagcgtgct
     1021 cggcgcccgg ccgatctccg agccgcggac gacttgccag acggccgact gcgcctcgta
     1081 gctggagccg gcgacgtaga cggtcgtctt ggcgacgttg tccggacggg cgccgccggc
     1141 ctcgagcgcg gcgagcaggt tctcgatcac ctgctcggcc tgggcgacgg ggtcgtcccc
     1201 gcccaccagg tcgccttgcg cgtcgagtgg caccgctccc gccgtgaaca cgacggtccc
     1261 tccgcgtccg accgcgacgt gcgagtagcc gggtggcggg gcgagggcgt ggacgttgcg
     1321 aagctcgacc gccatgtcag cccgccagct gggtcggtcc ctcgacgagg atccattcgg
     1381 cgcgggtgac ggcctcgctc cggtcgaagt cgaagacccc gctccacggg accccttccc
     1441 aggtcgaacc gcattcgggg cagacccaca gggacatcgt cgtgtgctgg gcgtcgcgcg
     1501 gcggtgcgca ggtgtgcagc ggcatccggc ctcctccccg ttcgaatcac acggttgtga
     1561 ttcggaggga gtgtacaccc cgggaccgcg ttccctcgag cgtcttggcg ggttcccacc
     1621 ccacgtccgg tcgtggcacc acccttccct ttccggcccc acggcggcat cgttcgctcg
     1681 tgagcatctc gtcgacgctc gagcgtccgc gccggcggga ggcgctcccg agcgccctcc
     1741 gagggttcgc ggccgtgttc gtggccggtg tcgtggcggg ggtcgtggtc ggcggcgtcg
     1801 gcagccggat cgcgatgcgc atcgctgcgc tcaccggccg ggacgccgcc cagggcctca
     1861 ctaccgaggc gggagcgacg atcgggagga tcacgttcga gggaacggcc ttcctggtcg
     1921 tcgcggtcgg catcgggtcg gcgctggtcg ggacggcgtt ctacctggcg acgctcccct
     1981 ggctcccccg gcgacggacg ccgcgtgcgc tggcgttcgg ctacctcgag ctcgcggtct
     2041 tcggcagcgt gctgttggat cccgggaacc cggacttcac gatcctggga aggccgctgc
     2101 tcgacgtcct cgtgctcggc tcgctgttcg tcctccacgg ggtggcgctc gtgatgctcg
     2161 tcgagccgag cgggcgactc gtgtccgcgg tcgcgcgagg cggccgttgg cgcgctcggc
     2221 tggtggccgt gggcacgttc gtcgcgatgg ccctgacggc gctcggcgcc ttggcccttc
     2281 tggctcgtgc cactggatgg gcggcgcccg tgacgctcgt cctgctgctc tgcgccgcgg
     2341 gtctgctggt cctggatccg cgcccaacgc gaccggtcac gcgtcccgcc ctgagggtca
     2401 cgggcgcggt cgccttgacc ctcctgacga tctcgggaac cgtcgggttg gtcgacgccg
     2461 tgacgacgat cgtctgatcg ctaggagcgg ggccggtcct tgttggtgtc gtagatcggc
     2521 agaacgtcga cggtcgcgtc gaccgtgccg tcgcccatgg ccacctgcac ggtgttcccg
     2581 acgaccgctt ccacggtcgg cacgatcgcg atgccgatct tcccgaacct gggtgtctcg
     2641 gtcgggctcg tcagcacgcc gatctcctcg ccgcccttgg tgaccgcggc gccgtactcg
     2701 ggcagtgcgt ccgcctcgta gcgcagggtc accagccggt tgggcggtgt cgccgcgacg
     2761 tcgcggagct tctccttgcc gaggaactcg cccggcgcgt ccagccggac gaaccggtcg
     2821 aggttgaagt cgtagggcgt ccgctggtgg gcctcgtagt cgtagtcggt gacgatcagc
     2881 cccgcctcga tacgagcgag ctcgatcgcc tcggccccga acggcgtggc gccggccccc
     2941 accacggcgt tccagagctc ctccgcatcc gacgggtcgg tcaggaacag ctcgaacccc
     3001 agctcgccgc cgtacccggt gcgcgagagg tagacctcga cccgggccac cttcacgggc
     3061 tcggggagga accggaagta gcggagggcg tcaacgtcgg cgtcggtgag cttccgcacg
     3121 acctcgcgcg agcgcggtcc gatcacgccc aggtgtggca tccgcggcgc gatccactcg
     3181 aactcgacgt cgaggtcccg cagggcctcg ccgaagtagt cgtcgtggtc cttcccgttg
     3241 gtcatcaccc agcagtgacc ggcgccggtg ttgaacacgg tcccgtcgtc gaccatcagc
     3301 ccgtcggtgt ccaggaacgc cccgtagcgc gcctgtccga cgtcgagccc gagcgcgtcg
     3361 ttgctgaaca accgctgcgc ggcccggagc ccgtcggacc cgcggaggtc ccacttgttg
     3421 agcggcgaga catcccatac cgcgacgtcg tctcggatcg cggcatactc cttcacgacg
     3481 tcgccgaacc cctcgagcca cagccacccg ccgtcggcga tgtgcgtgcc gccctgagcc
     3541 tcggtcacgt cgtggaacgc cgtcctgatc gcgtcgtcgt cgcccatggc tgcctccggt
     3601 cgccgagatg gcggcatcct cccacgggtt tgactggccg ttcaagttcg ctgcggccga
     3661 cgcgaaccga tcagacccgg gtcagccgga ggaggctcct cgcggtgccg agaccgcgct
     3721 cctccagcct gtcggcgagc acgaggaaga gctgcgccgt caccatcgcg tcgtcgagcg
     3781 cctcgtgggg gttcgcgacc ggcaccccat agtgttccgc ggcagacgag aggctctgcc
     3841 gcacgtcctg atccaggtgc tcgagctcga tcgccatctg ccggacgtcg atcgtccggc
     3901 gcacccacga gcggcggcga ccgccgtaga ggcggcccag gaagccgatc tcgacgctgg
     3961 cgaaccacgc cagcaggaac cgcccggcca gggcgagtcg gagcgtctgc ccggccaccg
     4021 ggaacggctg cgcgtgcgcc agatccttgg gcaggatgcc gtggatcctc atcgactccg
     4081 cgctcgacgg cacctcgggc agcaccagct ggtgcacggc ctctcccacg acgacgcggc
     4141 cctcgcgcac cggaacgacc ccgaacgaca cgaccgcgtc gcgctcgcgg tccaggcccg
     4201 tcgtctcgaa gtcgagcgag gcgaactccg cctcgcgcca cggacgcgac cgcatcgacc
     4261 acggcggccg tgcgggcggt ctcagcgaca cctgatcacc tcgacctcgg tcacgatgcc
     4321 gtcgatgcgc cggtcgatcg caccgctcgg gacccggtcc acgatctgca gggcgaacgc
     4381 gatcgcgatc gcggccaccc catcgggccg cttcccaagg aggcggtcgt agtacccgcc
     4441 gccgtagccc acccggccgc acgagcgatc gaacgcgacc cccgggacca cgacgaggtc
     4501 gagctcggcg acgtcgagcg cgcggccctc cgccggttcc atcgctccga acgaggcctc
     4561 cctcattcgt gatccgggcg tggcgacgac gggggccacc tcgttcccct cgatccgtgg
     4621 cagcgcgacg gtcttgccct gcgaacgcag gcgcgcgatc agcggtcccg tatcgacctc
     4681 gctgccgaac gaccagaagg ccagcaccgt atcggcgccg gccgtctcgg ccaacccgag
     4741 aaaccggtcg gcgatcgcct cgctgcgctc ggaccgatcg tcctggggga cggcgtcgcg
     4801 ttgggccagg accgcgcggc gcagcgcccg cttggcctgt ttcagccggt gggcggggag
     4861 atcgctcacg ccgacgatcg cagctcgggg agcacctcct cggcgatcac gtccagccgg
     4921 tccggtggac cgccggggac gagcaccgac cacgtcgagc cggcgtccgc gagcgcacgg
     4981 aggtgggtcc ggagcgacgc ggtcgtcccc gaccagaccc cgggctccag gccgcgcgcg
     5041 ctccgctgct cgagcagtcg cccgacctcg gcgtggtcgc gcccgaccac gacgatgccc
     5101 gcccaggtcg ccgagaccgg ccgctccccc gcggcctcgc gcagcaactg caccttgcgg
     5161 gtgaactcct cgacccccat cccccacccg ttccacgcgt cggcctcggc ggccgcgagg
     5221 cgcaccaccg cgtcggcgaa ccccccgatc cagatcggcg gaccgtctgg gcgcacgggc
     5281 ggcggcagga gcggtcccgt catcgcggga acgtacgtac cgccgtccca ccggaccccg
     5341 ccgtacaggg ccttgatcgc gcgcacggtc tcgacgaggt gaccgcgacg ttccgccttc
     5401 cccaggtacg cgatgccgaa gacccggtgc tcgagctcgt cgatcgggtc ccccgtgccg
     5461 atcccaagga tcatgcgccc gccgccgacg tcgtcgaccg cggcggcgag cttcgcgagc
     5521 attcccgcgg gccggagcgt cgccctcgtg accagggtcc cgacggcgat gcggctcgtt
     5581 gccgccgcga cggccgcgag cgtcgcgtac gcctcgagcg aaggccggtc cggggccgcc
     5641 cccggcggga acaggtggtc gaacgcgaag accccgtcga acccgcgctc ctcggcgcgc
     5701 cgggcgaacg cgagcacgcg cccggcgtcg ccggagaaca tcggaaggac gagccccacc
     5761 ttcacgtcag accatcacgc gcccgagctc ctccagcgcg agggtggccg cccccacggc
     5821 cccggcatcg ttgccgagct ccgcgggcac gatcggcacg cgggggcgat gttcgggagc
     5881 ctcgaccgcg tcgagatacg cggcgcgcgc gggatcgagc aggagatcgc ccgcgacgat
     5941 cgcaccgccg ccgacgacga cgacctgcgg gtccaggacg ttcacgaggc cggcgatccc
     6001 ctggcccagc cgtcgtccgg cctccgagag gatccccctc gcggcctcgt cgccctcccg
     6061 cgccgcctcc gtgacgagct cgccggtcac ctcgtcgggg tcgccgccgg ccagacgacg
     6121 aaggatcgag tgcccgtgct cgcgagcctc ctcgcggccc atccgatcga tcgccctgcc
     6181 ggcggcgacc tgctcccagc agccgcggtt gccgcagccg cagagcgggc cgccgggctc
     6241 gacgatgatg tggccgatct cggcggcgaa gccgttcgcg ccgcggaaca gcctgccgtc
     6301 cgacacgatc ccgccgccga tcccggtgcc aaccgtgacg agcagcaggt gccggtaccc
     6361 gcgacccgcg ccgaaccgga actcgccgta cgctgccatg ctcgcgtcgt tgtccacctg
     6421 gcagggaagg ccgagcgcgt cgcgcatccg ttcggcgatc gggaggttgc gccaggcgag
     6481 gttgggggag aagcgcatga ccccttcctt cgcgtcgacc agcccggcgg cccccacccc
     6541 gatcgcggtc acgtccgggg tcgtgagcga ccgggcgagc tcgaccatcg tggacagcgt
     6601 cgccgcctcg tcctccgcgg gagtcggcga gctcgagcgc tcgacgatcg tgccgtcgcg
     6661 ggtgacacgg aacgcgttga tcttggtgcc gccgacgtcg agaccgatcg cctcgccgct
     6721 cccacgttcc ggcacgtccg aacctctcag gcggaacgga ccggcgtcgt cacgagatct
     6781 cgatccgctc gaagtccttg gagccgttcg ggctcttccc gacgccgctc acgtggtccc
     6841 cgcgcacgtc gatcacggcc cgcgccgcga ggaagaactc acgggcggcg gcgagcaggt
     6901 gctcgaacac gtcgggggcc gcaccctggc ccgcggtcac cgccatgcag atcgggcaga
     6961 acgccacggt gcacacgggc ggctgcggtt gggcctccga cgccgacccg gcggctccgt
     7021 cgccctccga ggccgcgtgg cccggttcgg tctcaggcat cttcagctcc ttcccctcgg
     7081 cgccgcagcc ggcgacgacc ccggcggccg cgcgaacacg atctcgagat ggtctccgac
     7141 gaagttggcg ccgcggacct ccatccgctg gagcgtctgc ggcaggatca ggttgcgctt
     7201 gtaggtcccg acgcggacgt agagct
//
LOCUS       DATBRK010000017         5750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_146735, whole genome shotgun sequence.
ACCESSION   DATBRK010000017 DATBRK010000000
VERSION     DATBRK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146735"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VIH70_00460"
     CDS             complement(<1..639)
                     /locus_tag="VIH70_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY8114862.1"
                     /translation="MLRAFVNAFKVPDLRNKIFFTLAIIAVYRLGSHVPVPVVDINIL
                     TDALNAQGSTGFLSFIDLFSGGALTRMAIFGLGIMPYITASIIMQLLTVVIPKLESWH
                     KEGESGTKKINQWTRYVTVVLALLQSTGLVFLFHSRSDQLGGVDIFPTGTFTAANIAL
                     IVLVMTAGTAMIMWMGELITQRGIGNGMSILIFSSVISRLPQEGRAILLQAGN"
     gene            complement(651..1094)
                     /gene="rplO"
                     /locus_tag="VIH70_00465"
     CDS             complement(651..1094)
                     /gene="rplO"
                     /locus_tag="VIH70_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY8114863.1"
                     /translation="MKLHHLKPAEGAKRDRTRVGRGRAGTRGKTAGRGTKGWGARHNP
                     KPGFEGGQMPLQRRVPKLKGFTNPNRVEYAVVNVGSLGGVFEGEVDPEALLEHGLVRK
                     GRPVKILARGELDKALTVRAHAFSEAARAKIEGAGGRAEVVGEQG"
     gene            complement(1091..1330)
                     /gene="rpmD"
                     /locus_tag="VIH70_00470"
     CDS             complement(1091..1330)
                     /gene="rpmD"
                     /locus_tag="VIH70_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY8114864.1"
                     /translation="MTKLKVTQVRSVIHRPKDQKQTVRRLGLHRIRDSVIKDDRPEIR
                     GMIAKVHHLVHVEEVPGDGKDEGGVSGRDRGGTSS"
     gene            complement(1327..1953)
                     /gene="rpsE"
                     /locus_tag="VIH70_00475"
     CDS             complement(1327..1953)
                     /gene="rpsE"
                     /locus_tag="VIH70_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY8114865.1"
                     /translation="MRRYDSQGEKSIYEERVISINRVAKVVKGGRRFSFAALVVVGDG
                     AGRVGLGYGKAKEVPAAIAKGVEEAKRKMFEVPMMGTTIPHQVTGRDGAGIVLLKPAA
                     PGTGVIAGGAVRALVECAGIKDILSKSLGSSNAINIVKAAMTGLQSLRRPEEIARLRG
                     RELHEVAPKPMLDAVAAAQARMSATRAAKLEEVYGDQAQASPDAGGRR"
     gene            complement(1953..2396)
                     /locus_tag="VIH70_00480"
     CDS             complement(1953..2396)
                     /locus_tag="VIH70_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY8114866.1"
                     /translation="MDAKTRRRARVRRHDRVRRKIAGTAERPRLAVYRSNRHIYAQLV
                     DDDTARTLIAASDRDVRVGGAGKAGGAGKAGGAGKADAAGKADAAARAGGRGKTAPAK
                     AVGELLAERAKAAGIDRVVFDRGGRLFHGRVAALAEGAREKGLQI"
     gene            complement(2396..2947)
                     /gene="rplF"
                     /locus_tag="VIH70_00485"
     CDS             complement(2396..2947)
                     /gene="rplF"
                     /locus_tag="VIH70_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY8114867.1"
                     /translation="MSRIGKQPIEIPSGVDVTVGEDAVVVVKGPRGQLTQRVHPEMRL
                     VREDDVLRVERPTDEGFHRSLHGLTRSLIANMVEGVTKGYEKRLAIVGVGYRAAMKGK
                     ALEVQVGFSHPVTVPAIEGIEFETPTPTQIVVRGNDKQLVGEVAANVRKIRKPEPYKG
                     KGIRYENEYVRKKAGKAAKGATA"
     gene            complement(2964..3368)
                     /gene="rpsH"
                     /locus_tag="VIH70_00490"
     CDS             complement(2964..3368)
                     /gene="rpsH"
                     /locus_tag="VIH70_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY8114868.1"
                     /translation="MATNVNDPVGDMLTRVRNANLAYKDDLVVPASKMSKALLQILTT
                     EGYVSDFAEEGEGIERVYRVTLKYDAKRKRTISGIRRVSKPGRRVYAGRGDLPRVLGG
                     LGIAIISTSQGVMTDRDASRKGIGGEVLAFVW"
     gene            complement(3371..3556)
                     /locus_tag="VIH70_00495"
     CDS             complement(3371..3556)
                     /locus_tag="VIH70_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY8114869.1"
                     /translation="MAKKALRNKQEAQAKFKVREYTRCRRCGRARAVYRRFLLCRICF
                     RELAHAGELPGVTKASW"
     gene            complement(3568..4143)
                     /gene="rplE"
                     /locus_tag="VIH70_00500"
     CDS             complement(3568..4143)
                     /gene="rplE"
                     /locus_tag="VIH70_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015650399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY8114870.1"
                     /translation="MTDRPTPRLKERFNQEIRTRLQQELGFANPNQVPRLEKVVVNMG
                     VGDALKDGRMLEAAVDDLTIITGQKPVITKARKSIAGFKLREGSPIGAKVTLRGDRMW
                     EFIDRLVSIAIPRIRDFRGLNPNAFDGHGDYTLGLTEQLIFPEIDYDKVLKIRGMDIT
                     VVTTARNDDVGRALLVALGFPFEGIPMAAAS"
     gene            complement(4140..4478)
                     /gene="rplX"
                     /locus_tag="VIH70_00505"
     CDS             complement(4140..4478)
                     /gene="rplX"
                     /locus_tag="VIH70_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY8114871.1"
                     /translation="MPGVNINKDDTVKVMTGKSAGHTGRVIRVLPRDGRVMVEGAAMA
                     KKHQRTSGKRSASGSQLQQGGIIDTELYIDISNVMLVCNSCGQPTRVGHRIEGDGSKF
                     RVCRKCGADQ"
     gene            complement(4480..4851)
                     /gene="rplN"
                     /locus_tag="VIH70_00510"
     CDS             complement(4480..4851)
                     /gene="rplN"
                     /locus_tag="VIH70_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY8114872.1"
                     /translation="MIQQESRCKVADNTGAKEVLVIRVLGGSGRRYAHVGDVVVGSVK
                     DALPGGGVKKGEVVKAVVVRTAKERRRLDGSYIRFDDNAVVILTADSKNPRGTRIFGP
                     VARELRERRFMKIISLAPEVL"
     gene            complement(4852..5139)
                     /gene="rpsQ"
                     /locus_tag="VIH70_00515"
     CDS             complement(4852..5139)
                     /gene="rpsQ"
                     /locus_tag="VIH70_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY8114873.1"
                     /translation="MPARTAPEGSAGDRTRRKVRVGVVVSDKMDKTVLVRIDRRVRHP
                     LYKKTVARSNKLAAHDETNDAHVGDTVRLMETRRTSKSKRWRVVEIVERAK"
     gene            complement(5214..5447)
                     /gene="rpmC"
                     /locus_tag="VIH70_00520"
     CDS             complement(5214..5447)
                     /gene="rpmC"
                     /locus_tag="VIH70_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY8114874.1"
                     /translation="MATIRELRDLGDEELFERLESDKEELFNLRFQLATGQLDNPMRI
                     KDVRHEIARILTIVSERASAQRNEARGTEAEAS"
     gene            complement(5447..>5750)
                     /gene="rplP"
                     /locus_tag="VIH70_00525"
     CDS             complement(5447..>5750)
                     /gene="rplP"
                     /locus_tag="VIH70_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003781881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY8114875.1"
                     /translation="ITNRQIEAARVAITRHIRRGGKVWINIFPDKPVTKKPAETRMGS
                     GKGNPEGWVAVVKPGRVMFELSGVSETLAREAMSRAAHKLPIKSKFITRAGIGGME"
BASE COUNT          769 a         2127 c         1822 g         1032 t
ORIGIN      
        1 gttgccggcc tgcaggagga tcgcccgtcc ctcctggggc aatcgcgaga tcacgctcga
       61 gaagatcagg atcgacatcc cgttgccgat cccgcgctgg gtgatcagct cgcccatcca
      121 catgatcatc gcggtgccgg ccgtcatgac cagcacgatc aacgcgatgt tcgccgcggt
      181 gaacgtcccc gtcgggaaga tgtccacgcc gccgagctgg tccgaacgcg agtggaacag
      241 gaacacgagg cccgtggact gcagcagcgc cagtaccacg gtcacgtagc gcgtccactg
      301 gttgatcttc ttcgtgccgg actcgccctc cttgtgccag gactcgagct tcgggatcac
      361 gacggtgagc agctgcatga tgatcgaggc cgtgatgtac ggcatgatcc cgagcccgaa
      421 gatcgccatg cgcgtcagcg cgccgcccga gaacaggtcg atgaaggaga ggaacccggt
      481 gctcccctgt gcgttcagcg cgtcggtcag gatgttgatg tcgacgaccg ggaccgggac
      541 gtgcgacccc agccggtaca ccgcgatgat ggccagggtg aagaagatct tgttccggag
      601 atccggaacc ttgaacgcgt tgacgaaggc gcggagcacg ggtccctatc ctacccctgc
      661 tcgcccacga cctcggccct gccgccggct ccttcgatct tcgccctggc cgcctcgctg
      721 aacgcatgcg cgcggacggt gagcgccttg tcgagctcgc cgcgcgcgag gatcttgacc
      781 ggccgaccct tgcggaccag gccgtgctcg agcagggcct ccgggtcgac ctcgccctcg
      841 aacaccccgc cgagcgagcc cacgttgacg acggcgtact cgacgcggtt cgggttcgtg
      901 aagcccttga gcttgggtac gcggcgctgg agcggcatct ggccaccctc gaacccgggc
      961 ttcgggttgt ggcgggcgcc ccagcccttg gtgccgcggc ccgcggtctt gccccgcgtg
     1021 ccggcgcggc cgcggcccac gcgcgtgcgg tccctcttcg cgccctcggc gggcttgagg
     1081 tgatgcagct tcacgacgac gtccctcccc gatctcggcc gctcacgccg ccctcgtcct
     1141 taccgtctcc agggacctct tcgacatgga cgagatggtg gaccttggcg atcatgccgc
     1201 ggatctccgg gcggtcgtcc ttgatcacgc tgtcgcggat ccggtgcagc cccaggcgcc
     1261 ggacggtctg cttctggtcc ttcgggcggt ggatcacgct ccggacctgg gtgaccttga
     1321 gcttcgtcat cgccggccac ccgcgtcggg cgatgcctgg gcctgatcgc cgtagacctc
     1381 ctcgagcttg gccgcgcgcg tcgccgacat gcgcgcctgc gccgccgcga ccgcgtcgag
     1441 catcggcttg ggcgccacct cgtggagctc gcgcccgcgc agacgggcga tctcctcggg
     1501 gcgtcgcagg ctctgcagcc cggtcatcgc cgccttcacg atgttgatcg cgttcgagga
     1561 gccgagcgac ttggacagga tgtccttgat ccccgcgcac tcgacgagcg cacgcaccgc
     1621 gccgccggcg atcacgccgg tccccggcgc cgcgggcttg aggagcacga tgccggcacc
     1681 gtcccgaccg gtcacctggt gcgggatcgt cgtgcccatc atcggcacct cgaacatctt
     1741 gcgcttggcc tcctcgacgc ccttggcgat cgccgcgggc acctccttgg ccttgccgta
     1801 gcccagaccg acgcggccgg cgccgtcgcc caccacgacg agcgcggcga acgagaaccg
     1861 gcggccaccc ttgacgacct tggccacgcg gttgatcgag atcacccgct cctcgtagat
     1921 gctcttctcg ccctgtgagt cgtagcgccg catcagatct gcagtccctt ctccctcgca
     1981 ccctccgcga gcgcggccac ccgcccgtgg aacagcctgc cgccgcggtc gaacacgacg
     2041 cggtcgatcc ccgccgcctt cgctcgctcg gccagcagct cgccgacggc cttggccggc
     2101 gccgtcttgc ctcggccgcc ggcccttgcg gccgcgtccg cctttccggc cgcgtccgcc
     2161 tttccggccc cgcccgcctt tccggccccg cccgcctttc cggccccacc cacccgcacg
     2221 tcccgatccg aggcggcgat cagcgtgcgc gccgtatcgt cgtccacgag ctgcgcgtag
     2281 atgtgccgat tggagcggta gacggccaga cgcggccgct ccgcggtccc ggcgatcttc
     2341 ctgcgcacgc gatcgtgacg gcggacccgg gctcggcgtc tggtcttggc atccatcagg
     2401 cggtcgcacc cttcgcggcc ttgccggcct tcttgcggac gtactcgttc tcgtagcgga
     2461 tccccttgcc cttgtagggc tcgggcttgc ggatcttccg aacgttggcc gcgacctccc
     2521 ccacgagctg cttgtcgttg ccccgcacca cgatctgggt cggcgtcggc gtctcgaact
     2581 cgatcccctc gatcgcgggc accgtgaccg ggtgcgagaa ccccacctgg acctcgagcg
     2641 ccttgccctt catcgccgcg cggtagccga cgccgacgat cgcgagccgc ttctcgtagc
     2701 ccttcgtgac gccctcgacc atgttcgcga tcaacgatcg cgtgaggcca tggaggctgc
     2761 ggtggaaccc ctcgtcggtc gggcgctcga cccgcaggac gtcgtcctcg cggacgaggc
     2821 gcatctcggg gtgcacccgc tgcgtcagct ggccacgagg acccttgacg accacgacgg
     2881 cgtcctcgcc gacggtgacg tcgacgccgc tcgggatctc gatgggctgt ttgccgatcc
     2941 gactcatggc ctctgctccc cggtcaccag acgaacgcca gaacctcgcc gccgatcccc
     3001 ttgcgggatg cgtcgcggtc cgtcatcacg ccctgcgagg tcgagatgat cgcgatcccg
     3061 agcccgccga ggacccgagg cagatcgccc cgacccgcgt agacgcgccg gccgggcttg
     3121 ctcacgcgcc tgatcccgct gatcgtgcgc ttgcgcttgg cgtcgtactt cagcgtcacg
     3181 cgatacacgc gctcgatccc ctcgccctcc tcggcgaagt cgctcacgta gccctcggtg
     3241 gtcaggatct gcaggagcgc cttgctcatc ttcgacgccg gaacgacgag atcgtccttg
     3301 tacgcgaggt tggcgttgcg gaccctcgtc agcatgtcgc cgaccgggtc gttgacgttg
     3361 gtcgccagct tcaccatgag gccttggtca cccccggcag ctcccccgcg tgcgcgagct
     3421 cgcggaagca gatgcggcag agcaggaacc gccggtagac ggctcgggcc cgaccgcagc
     3481 gccggcagcg cgtgtactcg cgcaccttga acttggcctg cgcctcctgc ttgttcctca
     3541 acgccttctt cgccatgtcg cgtctcctta ggacgccgcc gccatcggga tcccctcgaa
     3601 ggggaacccg agcgccacga gcagcgccct gcccacgtcg tcgttcctcg cggtcgtcac
     3661 gaccgtgatg tccatccccc ggatcttcag gaccttgtcg taatcgatct ccgggaagat
     3721 cagctgctcg gtcagcccga gcgtgtagtc gccgtgcccg tcgaacgcgt tcgggttcag
     3781 ccctcggaag tcccggatcc tggggatcgc gatcgagacg agccgatcga tgaactccca
     3841 catccggtcg ccccgcaggg tgaccttggc gccgatcgga ctgccctcac gcagcttgaa
     3901 cccggcgatg gacttccggg ccttcgtgat caccggcttc tggccggtga tgatcgtgag
     3961 gtcgtcgacc gcggcctcca gcatacggcc atccttcagg gcgtcaccaa cccccatgtt
     4021 caccacgacc ttctccaagc gcggcacctg gttcgggttg gcgaacccca gctcctgctg
     4081 caggcgggtc cggatctcct ggttgaagcg ctctttcagg cggggcgtcg gccgatcggt
     4141 cattggtcgg ccccgcactt gcggcacacg cggaacttcg agccgtcgcc ctcgatgcgg
     4201 tgaccgaccc gggtcggctg gccgcacgag ttgcacacga gcatcacgtt cgagatgtcg
     4261 atgtacagct cggtgtcgat gatgccgccc tgctggagct gggaaccgct cgccgagcgc
     4321 ttgcccgacg tgcgctggtg cttcttggcc atcgccgcac cctcgaccat cacccggccg
     4381 tcgcggggga ggacgcggat cacgcggccc gtgtggccgg cgctcttgcc ggtcatgacc
     4441 ttcacggtgt cgtccttgtt gatgttcacg cctggcatgt cacaacacct ccggggcgag
     4501 cgagatgatc ttcatgaagc ggcgttcgcg gagctcacgg gcgacgggtc cgaagatccg
     4561 cgtgccgcga gggttcttcg agtcggccgt caggatcacc accgcgttgt cgtcgaaccg
     4621 gatgtacgag ccgtccaggc ggcgtcgctc cttggcggtc cgaacgacga cggccttgac
     4681 gacctcgccc ttcttgacgc cgccgccggg cagggcgtcc ttcaccgagc cgaccaccac
     4741 gtcgcccacg tgcgcgtacc ggcgaccgct cccgccgagc acgcggatca cgaggacctc
     4801 cttggccccc gtgttgtccg ccaccttgca acgcgattcc tgctggatca cttacttggc
     4861 cctctccacg atctcgacca cgcgccagcg cttgctcttg gaggtccgtc gtgtctccat
     4921 cagccgaacg gtgtcgccga cgtgcgcgtc gttcgtctcg tcgtgcgcgg ccagcttgtt
     4981 cgagcgcgcg acggtcttct tgtacagagg gtgccgcacg cggcggtcga tccggacgag
     5041 cacggtcttg tccatcttgt cggacacgac gacgcctacc cggaccttgc gccgggtccg
     5101 atcccccgcg gagccctccg gtgccgtccg cgctgggatc gccgacccgg cggcctcgcc
     5161 gagtgtggta tcgggcgtgg cctggtccgt cacagtctcg tcgggtcgtt cgctcatgat
     5221 gcctccgcct cggtcccccg ggcctcgttc cgctgcgcgg atgcgcgttc gctcacgatc
     5281 gtgaggatcc gcgcgatctc gtgccggacg tccttgatcc gcatcgggtt gtcgagctgc
     5341 cccgtcgcga gctggaaccg caggttgaac agctcctcct tgtcggactc gagccgctcg
     5401 aacagctcct cgtcgccgag gtctcggagt tcccggatgg ttgccatcac tccatccccc
     5461 cgatgcccgc gcgcgtgatg aacttgctct tgatcggcag cttgtgcgcg gcccgcgaca
     5521 tcgcctcgcg cgcgagcgtc tcggagaccc cggacagctc gaacatcacg cgccccggct
     5581 tgacgacggc gacccagccc tccgggttgc ccttgcccga acccatccgg gtctcggccg
     5641 gcttcttcgt cacgggcttg tcggggaaga tgttgatcca caccttcccg ccgcggcgga
     5701 tgtgacgcgt gatcgcgaca cgggccgcct cgatctggcg gttcgtgatc
//
LOCUS       DATBRK010000018        13208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_434795, whole genome shotgun sequence.
ACCESSION   DATBRK010000018 DATBRK010000000
VERSION     DATBRK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434795"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VIH70_00530"
     CDS             <1..406
                     /locus_tag="VIH70_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8114876.1"
                     /translation="IITAFSMLLGFRFEGGSAGAFGALLLVLAFGLVMCWPLAFIGIT
                     ARTTESVNTWGFMIILPLTFASSAFAPVDTMPGWLQAFVKANPVTSIIDAARGLMLGG
                     PVAEPVIDSVIWMIVITAVFAPLAILRYRRRV"
     gene            436..939
                     /locus_tag="VIH70_00535"
     CDS             436..939
                     /locus_tag="VIH70_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114877.1"
                     /translation="MALVHTTDDGRHDFDFLFGRRRIHNRKLVDTLDPDSTEWVEFEA
                     FGEAGPILGGLGNVDTFVAPAMPPGGDPFDGFTLRLFDPETGIWRIWWASIRFPGVLD
                     RPVEGRFDGDRGLFECDDVIGGKPVRVRYDWSVLSPTANRWEQSFSYDVGVTWRTNWI
                     SEGTQVS"
     gene            1029..2063
                     /locus_tag="VIH70_00540"
     CDS             1029..2063
                     /locus_tag="VIH70_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015579351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8114878.1"
                     /translation="MLDAVERLVGMQAQVPRDPYVALWSRLDRFRPEALAGPIAERLA
                     VRMGLLRATLHLVTARDALALRPILEPVMRRTLYSQSPFGRALDGLDVDDLKAFATRL
                     LVERPRTRAELIPFLAERWPEHDATQLSYAAMYLLPLVQVTPRGIWGRTGRSAFTTVE
                     HWLGRPVDAETAPDEMVLRYLAAFGPATPGDARSWSGLAGMREVLERLRPRLRTFRDE
                     RDRELFDVRGAPLPDPDTPAPVRFLPEYDNVQLGHDDRGRIAPPGLRGSTEVGWGSIL
                     VDGFGAARWRAELDGDRATLWVEAFSKLARSDRAEISEEGERLAAFLAPDAGRRDVRF
                     RTFGGSAPSG"
     gene            2060..3265
                     /locus_tag="VIH70_00545"
     CDS             2060..3265
                     /locus_tag="VIH70_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8114879.1"
                     /translation="MRRVGVRERRARLGARHRLASRAATVEEAAGAVVGLHSSDPATV
                     YLSSWARVDGFEPTDLEDALYDRRSLVRMLGMRRTLFVVPVDLAPVMHEACTKALIPG
                     QRARLVRMLEEERVAPRGRGEAWLAKVSAETLAAIAARGEATARELTADIPELGAKLT
                     FGQGKTWAGTVGVSTRVLFLLATQGPIVRTRPLGTWTSGQYRWATTEHWLGAPLREID
                     HAEACSKLLGRWLRSFGPAKMTDIRWWTGWSAALAKRTLGAIDAVEVRLADGTGYVLE
                     DDLDRVDDADPWVALLPGLDPTTMGWKDRRWYLGERATDLFDRNGNAGPTVWANGRVV
                     GAWAQTDEGELVVEVFARLDAATRALLDAERVRLAAWLGDVRIRPRFGTPLGKELATR
                     SSGGASRGR"
     gene            complement(3277..3462)
                     /locus_tag="VIH70_00550"
     CDS             complement(3277..3462)
                     /locus_tag="VIH70_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114880.1"
                     /translation="MTAQDSEMKAAAAAAKAREVIANRIAKLVPKTEDDQVASVVGHL
                     AEAYANLAVEPPRSRTG"
     gene            3580..4047
                     /locus_tag="VIH70_00555"
     CDS             3580..4047
                     /locus_tag="VIH70_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8114881.1"
                     /translation="MSNAADLLGEVRLRAVTEDDLPIFFEHQRDPVAYRMAAFAPRER
                     DAFMKHWGSILRDASSIERTIELDGHVAGNIVLFGFEGRREVGYWIGREFWGRGVATR
                     ALSAFLREVEERPIYAGVVETNVASIRVLDKCGFTAVETDDEEHAGEILLRLG"
     gene            complement(4061..4852)
                     /locus_tag="VIH70_00560"
     CDS             complement(4061..4852)
                     /locus_tag="VIH70_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEY8114882.1"
                     /translation="MALDLDAYLERIGYEGPVEPTLEVLDAVHLAHATTIPFENLDIL
                     LGRPIRLDLPSLQDKLVRGRRGGYCFEHNTLFAAALEAIGFGVTRLGARVRLGARHVT
                     ARTHMALLVRAGDEGRLADVGFGGGSILCSLPMEPGRAVDQFGWGFRLVDEDGARVVQ
                     TRRPRGWFDLYAFTFEPQYPVDYEMANYFTSTHPSSPFTRTLTAQLCAPERSLILRGR
                     KLIEETPTGETVGEVRDDELLDVLAERFTLSFPPGTSFDPARAGS"
     gene            complement(4866..5342)
                     /locus_tag="VIH70_00565"
     CDS             complement(4866..5342)
                     /locus_tag="VIH70_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8114883.1"
                     /translation="MAETDRRPGGVKELGHLVLYVRDLERSVRFYVDVLGWRRVGPGD
                     GLPFPAAMFSGGRTHHELLLIEVGPDATPIPAGRRVGLYHFGLKVGDTDDELRAALAR
                     LQEAGVPLMGTADHGMTHSLYLADPDGNEIELYIDVEGVDWDDPEVLMARPQALRL"
     gene            complement(5420..6610)
                     /locus_tag="VIH70_00570"
     CDS             complement(5420..6610)
                     /locus_tag="VIH70_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8114884.1"
                     /translation="MLVVVLGALVAACSSDDGAPPVTGRASSGGDGPTTPAASPSAGP
                     TPRRPDLEDARIRLVPVAELEQPVAMAVRAGDRELYVAEKVGRVVALTPGSDPRVVLH
                     LTNRVSLGSEQGLLGLAFSPDGRYLYVDFTDTEGDTNVAEFAFDDDGADPESERPVLF
                     VDQPFSNHNGGALAFGPDGYLYVALGDGGSGGDPMGNAQSLSTLLGKLLRISPRPSGG
                     EPYAIPPDNPFVGRSGARPEIWAYGLRNPWRFSFDAETGDMWIGDVGQNAWEEIDLEP
                     AASDGGSNFGWARLEGTHPFEGGSAPGSVPPVFEYANGGGSCAVTGGYVYRGELIPAL
                     AGAYVFADYCGGRLEAFVPRNGRATQARFLGPRVDALASFGEDARGELYVLSLAGTVF
                     RIAA"
     gene            complement(6696..7187)
                     /locus_tag="VIH70_00575"
     CDS             complement(6696..7187)
                     /locus_tag="VIH70_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY8114885.1"
                     /translation="MTDAVGTGVDIRDYCFAPTILRVSTGDAVTFTNVDPFPHSVLGA
                     NATWGDYAGFKNESVTYRFSEPGVYPYLCTYHVGMVGAVVVGDGVGGAIDTSTADGPV
                     TKVNASDLRLENTSAVDDQPPVADVGWPVIVAAAFGIVVGASALIVVRRRRSPTTRTR
                     EGR"
     gene            complement(7494..7847)
                     /locus_tag="VIH70_00580"
     CDS             complement(7494..7847)
                     /locus_tag="VIH70_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114886.1"
                     /translation="MGITLILLGLAAAGLVVDFAVENWSAAANDVTFSLFGGSFTATQ
                     VEVAIAAAVLGAIAIALCVLGAGLLRGSRGRRRTARRRMSELERENAALRDRPTDEDH
                     PVVHVDDRQPVQSQR"
     gene            7959..8804
                     /locus_tag="VIH70_00585"
     CDS             7959..8804
                     /locus_tag="VIH70_00585"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8114887.1"
                     /translation="MSTEPTPQEEWAAFLSGTHPHLQDKSPLRFIPSSPRCRICSAPF
                     GRPGAFVLRRYGYTPWEKNPKICGRCFKGIETVAKMCPTQGEDGEIHGAEVELSMLFA
                     DVRGSSRLARQLPTWEFTRLMNRFYRVSKDVLVEADAIIEKFVGDEVVALFLPLMTGP
                     DHARRAVESAEALLRATGHGDHEQPWVPLGAGVHTGVAFVGVVGTKGSSDFTALGDPV
                     NIASHLASQAAAGEILMSDATAEAAAMAHGDLERRHVSLKGHPLDALVLHVEGPVQTG
                     GPADG"
     gene            8857..11733
                     /locus_tag="VIH70_00590"
     CDS             8857..11733
                     /locus_tag="VIH70_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HEY8114888.1"
                     /translation="MSAIQRTLKIRPDETRLVLSMCALTFVGMAGLAIGQSGANALFF
                     DRIGTDALPLMYLAQGATAFVFMMGLAALLGRVDRRRAYLAIPAGLGAVVLAERLALL
                     TDGRWIFPVLWLTVALALLLQNVFVWGIAGVVTDTRRAKRLFPLFAAGEILGSVAGGL
                     FTDPLVGVIGTENLLLVWAAALAISFALCRVVLSSAGTLAQPRVRRARRREASPWREL
                     TVAFGYVRRSRLLVWMTLAAVLFSVLFYSLYLPWAAAATERFPDAADLAGFFGLFWAA
                     MTGAAFLVSVLVTNRLFGRFGIASMMLVLPLLYVGSFGILLVSAGFVTLVILRFVDGV
                     WLQGIASPAWETLTNVVPDARRDQVRTFLNGGPAQAGTAIAGIIALVGQDVLTPRQFA
                     AIGLATAAIAVFVTWRVRASYAPALVEALREGRPEVFPDVAVAGVPFAFAPDAQTLGS
                     LLVAMEDGQPTVRRLSVQLLADVDDPRATAALERALRDDDASVRAQALDGLAATPGRV
                     GAETLLPLLDDPDPAVAASAAAAVSTGTHTPDAARVLLRLAAHADPSVRSTALRHLVA
                     VPADAAAGLAAPALDDEDSGVRSAALTTLAAVRPDEALGPAIRGFVDPSARVREAAVA
                     AAVRIGDGSVDAVLDALARPAARDAALRALARLDLGGRDERVRAFVAERSRAATGDDA
                     LAAAVPSDGEAPGLLKDALLDRARANGRSALRALSLISSDGAAVRSAVESLDARDPEQ
                     VANALETLEATTDASLAASILALWEPTGGARRNLPEDWLERASADDDAFVAACAGAVT
                     VQRGGGDGMTRRATTTPVIERVLFLRRVPLFEGLAPADLGAIAEVAEERSFADGELLA
                     SEGELGDELLIVASGTVRVEAGGSEIARRGRGEVLGEMSLITRGPRVASLVADGDVRA
                     ICIGRREFESMIHDRPDIGIGVMRVLAQRLAEARPAEPSPASNA"
     gene            complement(11741..12688)
                     /locus_tag="VIH70_00595"
     CDS             complement(11741..12688)
                     /locus_tag="VIH70_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY8114889.1"
                     /translation="MRENRARSAHRAFLAVAFVACAIGIASVPSAFAQDEGATPGDDD
                     QVVLTGELLVPKGATVGTAVILNGDATIDGTATESVVVFNGDVEITGTVGDDIVAFNG
                     RVIVRSGAEVGGDIASRQDPRVEEGATVRGQIKGISGRFDLEGLGWVGRYVWWFGYTI
                     STLILGLVLLLFLPGVDAPLADAFRRRMGASFGFGAVIFFLLPIAAVLLLVVVIAIPL
                     GLFLLLALALLYTLGYVAGAHLLGRRLVNQPGSRYVAFLVGWLILRGIGLIPFLGGLV
                     WVVASIGGLGALVIAARRSTSVPAPSLPAAPIPEPPTAA"
     gene            12937..13140
                     /locus_tag="VIH70_00600"
     CDS             12937..13140
                     /locus_tag="VIH70_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY8114890.1"
                     /translation="MAIGTVKWFSPEKGFGFITPDDGTADVFVHFSAIAGEGFRNLEE
                     NQKVEYDVTQGQKGPQAANVRAV"
BASE COUNT         1811 a         4745 c         4751 g         1901 t
ORIGIN      
        1 gatcatcacg gccttctcga tgctgctcgg gttccggttc gagggcggct cggcgggggc
       61 cttcggggcg ctcctgctcg tgctcgcgtt cgggctcgtg atgtgctggc cgttggcgtt
      121 catcggcatc acggctcgaa ccaccgagtc ggtgaacacc tggggattca tgatcatcct
      181 gccgctcacg ttcgcgagct cggcgttcgc gcccgtggac acgatgcccg ggtggttgca
      241 ggcgttcgtg aaggcgaacc ccgtgacgtc gatcatcgac gcggcacgcg gactgatgct
      301 cggcggaccg gtggccgaac ccgtgatcga ctcggtgatc tggatgatcg tgatcacagc
      361 ggtgttcgcg ccgctcgcga tcctgcgata ccgccgcagg gtctgaggaa gcttcgcagg
      421 atcggaggac tcgacatggc actggtgcat accaccgacg acggccggca cgacttcgac
      481 ttcctgttcg ggcggcggcg gatccacaac cgcaagctcg tcgacacgct ggatccggac
      541 tcgacggagt gggtggagtt cgaggcgttc ggggaggcgg ggccgatcct gggcgggctc
      601 ggcaacgtcg acacgttcgt ggctccggcg atgccgccgg gcggtgaccc cttcgacggg
      661 ttcaccctcc ggctgttcga cccggagacc gggatctggc ggatctggtg ggcgtcgatc
      721 cggttcccgg gggtcctcga ccggccggtg gagggtcgct tcgacgggga tcgtgggctg
      781 ttcgagtgcg acgacgtgat cggtggcaag ccggtgaggg ttcgttacga ctggtccgtc
      841 ctgtctccga cggccaaccg ctgggagcag tcgttctcct acgatgtggg cgtgacctgg
      901 cgcacgaact ggatcagcga agggacgcag gtcagctgac gtcatccgca ccgcgcgtcc
      961 tcgggcgccg cgcgctcaac cgcgcgctgc tcgaacgtca gctcctgctc cgacggcgaa
     1021 gggtcggcgt gctcgacgcg gtcgaacgcc tggtgggcat gcaggcacag gtgccgcgcg
     1081 atccttacgt cgcgctgtgg tcgcggctgg atcgcttccg tcccgaggca ttggcggggc
     1141 cgatcgccga acgcctcgcc gtgcggatgg ggttgctgcg ggccacgctc cacctcgtca
     1201 cggcgaggga tgccctggca ctccgaccga tcctcgaacc cgtgatgcgg cgcacgctct
     1261 acagccagag cccgttcggg cgtgcgctgg acgggctcga cgtcgacgac ctgaaggcgt
     1321 tcgccactcg gttgctcgtg gagcggccgc gcacccgagc cgagctcatc ccgttcctgg
     1381 ccgaacgctg gcccgaacac gacgccaccc agctctcgta cgcggcgatg tacctcctgc
     1441 cgctcgtgca ggtgaccccg cgcgggatct ggggccggac gggacgctcc gcgttcacga
     1501 ccgtggagca ctggctcggg cgtccggtcg acgccgagac cgccccggac gagatggtgc
     1561 tgcggtacct cgccgcgttc ggcccggcga cgccgggcga cgcccgttcg tggtccgggc
     1621 tcgcgggcat gcgcgaggtg ctcgaacgcc tccgcccgcg gctgcgcacg ttccgcgacg
     1681 agcgagaccg cgagctgttc gacgtccggg gcgcgccgct tcccgatccg gacaccccag
     1741 cacccgtccg cttcctgccc gagtacgaca acgtgcagct cggccacgac gatcggggcc
     1801 gcatcgcccc gcccggcctg aggggatcga ccgaggtcgg gtggggctcg atcctggtcg
     1861 acgggttcgg tgcggcgcgc tggagggcgg agctcgacgg ggaccgggcg acgctctggg
     1921 tcgaggcgtt ctcgaagctc gctcggagcg accgcgccga gatctccgag gagggggaac
     1981 ggctcgcggc gttcctcgcg ccggacgcgg gccgtcgcga cgtgcgcttc cggaccttcg
     2041 gcgggtccgc cccttcggga tgagacgcgt cggcgtccgc gagcgccgag cgcggctcgg
     2101 cgcgcgtcac cgccttgcgt cgcgcgccgc gaccgtcgag gaggccgccg gggcggtggt
     2161 cgggctgcac tcgagcgatc ccgcgacggt ctacctctcg tcgtgggcaa gggtcgacgg
     2221 cttcgaaccg accgatctcg aggacgcgct gtacgaccgc cgctcgctcg tccggatgct
     2281 cgggatgcgg cgaaccctgt tcgtcgttcc ggtcgatctc gccccggtga tgcacgaggc
     2341 ctgcaccaag gcgctgatcc ccggccagcg cgcgcgcctc gtccggatgc tcgaggagga
     2401 gcgggtggcg ccgaggggcc gaggggaggc gtggctcgcg aaggtctccg cagagacgct
     2461 cgccgcgatc gccgcgagag gcgaggccac cgcgcgcgag ctgaccgccg acatcccgga
     2521 gctcggcgcg aagctcacgt tcgggcaggg caagacctgg gccgggacgg tgggcgtgtc
     2581 gacccgggtg ctgttcctgc tcgccacgca gggcccgatc gtccgcacgc gcccgctcgg
     2641 cacatggacg tcgggtcagt accgctgggc gacgaccgag cattggctcg gtgccccgct
     2701 tcgcgagatc gaccacgccg aggcgtgctc gaagctcctc ggtcgctggc tccgttcgtt
     2761 cgggccggcg aagatgacgg acatccggtg gtggacgggg tggtccgcgg cgcttgcgaa
     2821 gaggaccctc ggagccatcg acgcggtcga ggtccgactg gccgacggca ccggctacgt
     2881 gcttgaggac gatctcgacc gggtcgacga cgcggacccg tgggtcgcac tgcttcccgg
     2941 gctcgacccg accaccatgg ggtggaagga ccgtcggtgg tacctcggcg aacgagccac
     3001 cgatctgttc gaccgcaacg ggaacgcggg tcccacggtg tgggcgaacg gccgggtcgt
     3061 cggggcgtgg gcgcagaccg acgagggtga gctggtggtc gaggtcttcg cccggctcga
     3121 cgccgcgacc cgcgcgctcc tggacgccga acgcgtgcgg ctcgcggcct ggctcgggga
     3181 cgttcggatc cggccgcggt tcggaacgcc gctcggcaag gagctcgcga cgcgttcgag
     3241 cggcggagcg tcccgggggc gctaggcgaa ctcggctcag cccgtccggc tgcgaggtgg
     3301 ttcgaccgcg aggttcgcgt acgcctcggc gagatgcccg accaccgagg ccacctggtc
     3361 gtcctcggtc ttgggcacca acttcgcgat cctgttcgcg atcacttccc gagccttggc
     3421 cgccgccgcg gccgccttca tctccgagtc ctgagccgtc atctacgaga tcacctccgg
     3481 ggcgatgcta cgcccgcgtc cgtgagcagc gctctctccc ctcgtccgga cgacgaccga
     3541 ccttgccgag gaccggatgg tgcgcgcagg atggtcggca tgtcgaacgc cgcagacctg
     3601 ctcggcgagg tgcggctccg cgccgtgacc gaagacgacc ttccgatctt cttcgagcac
     3661 cagcgggacc ctgtggccta tcggatggcc gccttcgcgc cgagggagcg cgacgcgttc
     3721 atgaagcact ggggttcgat cctgcgcgac gccagcagca tcgaacggac gatcgagctc
     3781 gacggccacg tcgccggcaa catcgtcctg ttcgggttcg aggggcggcg cgaggtgggc
     3841 tactggatcg ggcgcgagtt ctggggccgg ggcgtcgcca cgcgcgcgct ctcggcgttc
     3901 ctgcgcgagg tcgaggaacg gccgatctac gcgggggtcg tagagaccaa cgtcgcctcg
     3961 atccgcgtgc tcgacaagtg cggcttcacg gccgtcgaga ccgacgacga ggagcacgcg
     4021 ggcgagatcc tcctgcggct cgggtgatcg ccgggcccgc tcacgatccc gcgcgagcgg
     4081 ggtcgaagct cgtccccggc gggaacgaca gcgtgaaccg ctccgcgagc acgtcgagca
     4141 gctcgtcgtc gcgcacctca ccaacggtct cgccggtggg ggtctcctcg atcagcttgc
     4201 ggccccgcag gatcaacgag cgctccggcg cgcagagctg cgcggtgagc gttcgcgtga
     4261 acggcgagct cgggtgggtg gaggtgaagt agttcgccat ctcgtagtcc accgggtact
     4321 gcggttcgaa cgtgaacgcg tacagatcga accagccgcg tggccggcgg gtctgcacca
     4381 cgcgagcgcc gtcctcgtcg accagccgga acccccagcc gaactggtcg accgcgcgac
     4441 cgggttccat cggcaacgag cagagtatcg agccgccgcc gaacccgacg tcggcgagcc
     4501 tcccttcgtc gcccgcccga accagcagcg ccatgtgcgt tcgcgcggtc acgtgtcgag
     4561 cgcccaagcg gacccgcgcg ccgaggcgcg tcacgccgaa cccgatggcc tcgagcgccg
     4621 cggcgaacag cgtgttgtgc tcgaagcagt acccgccgcg ccggccccgg acgagcttgt
     4681 cctgcaggct cgggaggtcg aggcggatcg gccgccccag caggatgtcg aggttctcga
     4741 acgggatcgt cgtcgcgtga gcgaggtgga ccgcgtccag gacctcgagc gtcggctcga
     4801 ccggtccctc gtacccgatc cgttcgaggt acgcgtccag gtcgagcgcc atcgtgccgg
     4861 cccgcctaga gccgcagggc ctgcggcctc gccatcagca cctcggggtc gtcccagtcg
     4921 accccttcga cgtcgatgta gagctcgatc tcgttcccgt cgggatcggc caggtacagg
     4981 ctgtgggtca tgccgtgatc ggcggtgccc atcagcggca cgccggcctc ctgcagccgg
     5041 gcgagggccg cccggagctc gtcgtccgtg tcccccacct tgagcccgaa gtgatacagg
     5101 cccacccggc gcccggcggg gatcggcgtc gcgtccgggc cgacctcgat caggagcagc
     5161 tcgtgatgcg tgcggccgcc cgagaacatc gcggccggga acggcagacc gtcgcccgga
     5221 cccacccgac gccagccgag gacgtccacg tagaaccgca ccgaacgctc gaggtcccgc
     5281 acgtacagca cgaggtgccc gagctccttc acgccacccg gtcgccggtc cgtctccgcc
     5341 atcccgcccc ccgatcctgt cggtgtcctc cagaaccccg cgtccgcgcc gggtatgccc
     5401 gcggcgggag cgggcggcgt caggccgcga tccggaacac cgtccccgcc aaggacagca
     5461 catacagctc gccgcgcgca tcctccccga aggacgcgag ggcatcgacc cgcggcccca
     5521 ggaaccgagc ctgcgtcgcc cggccgttcc gcgggacgaa cgcctccagg cgcccaccgc
     5581 agtagtcggc gaacacgtag gcgcccgcca gcgccgggat cagctcgccg cggtacacgt
     5641 agccgccggt gacggcgcac gagccgccgc cgttcgcgta ctcgaacacc ggcgggaccg
     5701 atcccggggc cgatccgccc tcgaacggat gcgttccctc cagccgggcc catccgaagt
     5761 tcgacccgcc gtccgaggcc gcgggctcga ggtcgatctc ctcccacgcg ttctgcccga
     5821 cgtcgccgat ccacatgtcg ccggtctccg cgtcgaacga gaaccgccac gggttccgca
     5881 ggccataggc ccagatctcc gggcgcgctc ccgaccggcc gacgaacggg ttgtccgggg
     5941 gtatcgcgta cggctcgccc cccgagggtc tcggcgagat ccggagcagc tttcccagga
     6001 gcgtcgacag cgactgcgcg ttccccatcg ggtcgccgcc gctccccccg tctccgagcg
     6061 caacgtagag gtacccgtcc ggtccgaacg cgagcgcgcc cccgttgtgg ttcgagaacg
     6121 gctggtcgac gaacagcacc gggcgctcgc tctccggatc ggcgccgtcg tcgtcgaacg
     6181 cgaactcggc cacgttcgtg tctccctcgg tgtccgtgaa gtccacgtac aggtagcggc
     6241 cgtccggcga gaacgcgaga cctaggagac cctgttcgct cccgagcgac acgcggttcg
     6301 tcaggtggag cacgaccctc ggatccgaac ccggcgtgag cgccacgacg cgcccgacct
     6361 tctcggcgac gtacaactcg cgatcgccgg cccgcacggc catcgcgacc ggctgctcga
     6421 gctcggccac aggtacgagc cggatccgcg cgtcctcgag atcgggcctc ctcggtgtcg
     6481 gacccgcgct cggcgaggcg gcgggtgtgg tcgggccgtc gccgccggac gaggcccgcc
     6541 cggtcaccgg cggggcccca tcgtccgacg aacacgcggc gaccagcgcg ccgaggacga
     6601 cgacgagcat cagccttcgg cctcgagcga ttcccgccgg ccgaacgccg gtgggttcgg
     6661 ccgcggttgg ccgaggacga ctcacacgat cgagactacc gaccctcacg cgtacgcgtg
     6721 gtgggcgaac gccgccgacg cacgacgatc agggccgacg cgccgaccac gatcccgaac
     6781 gcggcggcca cgatcacggg ccacccgacg tcggccacgg gcggctgatc gtcgaccgcc
     6841 gacgtgttct cgagccggag gtcggaggcg ttcaccttcg tgacggggcc gtccgcggtc
     6901 gacgtgtcga tcgcgccgcc gacgccgtcg cccaccacca ccgcgccgac catcccgacg
     6961 tgatacgtgc acaggtaggg atacacgccc ggctcggaga accggtacgt gaccgactcg
     7021 ttcttgaacc ccgcatagtc gccccaggtc gcgttcgcgc ccaggaccga atgcgggaac
     7081 gggtccacgt tggtgaacgt cacggcgtcg cccgtcgaca cccgcaggat cgtcggcgcg
     7141 aagcagtagt cgcggatgtc gaccccggtc ccgaccgcgt cggtcaccgc tcggccgcac
     7201 ccgccccgcc gctcgccgat gcgaggccct ccccgagcac ggggacgagc accaacgccc
     7261 cgagcagcac caccccggcc gccgcggaca ccttccgtcg cctgcctcct ggccgcatcc
     7321 gggggcctcc tctcgctcgt tccccctgac acgagaccgg ccggacccgc ggcgggttcc
     7381 cgcgcctcct ccggctccgg gtcggattcg gcccggagcg cgacgcggtc ggagaccgtc
     7441 acgccggcga ccgggaggac cgggtcgccg gcgtgcggcg ggtggatgcg gggtcagcgc
     7501 tgggactgga cgggctggcg gtcgtcgacg tggacgacgg ggtggtcctc gtcggtcggc
     7561 cgatcccgga gagcggcgtt ctcgcgttcg agctcggaca tccggcgcct cgcggtccgc
     7621 cggcggccgc ggctcccacg caggagcccg gcgccgagga cgcacagcgc gatcgcgatc
     7681 gcgccgagca cggccgcggc gatcgccacc tcgacctggg tggccgtgaa cgagccaccg
     7741 aagagcgaga aggtcacgtc gttcgcggca gcggaccagt tctcgacggc gaagtcgacg
     7801 accagtccgg ccgccgcgag ccccagcagg atgagcgtga tgcccatcgg tcacctcccg
     7861 tctcgggagg ggtctctacc catcgggggc tcggctcatg ccccggcctt cgatcgtccg
     7921 ggacgtgcgc ggcgcgattg cctaggctgc acgctgagat gagcacggag ccgacacccc
     7981 aagaggaatg ggccgccttc ctgagcggca cccatccgca cctgcaggac aagagtccgc
     8041 tccgcttcat cccgtcgtca cccaggtgca ggatctgctc ggcaccgttc ggccgcccgg
     8101 gggcgttcgt gctccgccgc tacggctaca cgccctggga gaagaatccc aagatctgcg
     8161 gccggtgctt caagggcatc gagaccgtcg cgaagatgtg ccccacccag ggcgaggacg
     8221 gggagatcca cggcgccgag gtggagctct cgatgctgtt cgcggacgtc cgcggctcga
     8281 gcaggctggc tcggcagctg cccacctggg agttcacccg gctcatgaac cgcttctacc
     8341 gggtgtcgaa ggacgtcctc gtcgaggccg acgcgatcat cgagaagttc gtgggcgacg
     8401 aggtcgtggc tctgttcctc ccgctcatga ccggtcccga tcacgcacga cgcgccgtcg
     8461 aatccgccga ggcgctcctg cgcgcgacgg gacacggcga ccacgagcag ccctgggtgc
     8521 cgctgggtgc gggtgtgcac accggggtcg ccttcgtcgg cgtggtgggg accaaggggt
     8581 cgagcgactt cacggcgctc ggcgaccccg tcaacatcgc gtcgcacctc gcgtcgcagg
     8641 cggccgccgg cgagatcctg atgagcgacg cgacggccga ggccgcggcg atggcgcacg
     8701 gcgacctcga acggcgccac gtctccctca agggtcaccc gctcgacgcg ctggtgctgc
     8761 acgtcgaggg acccgtccag accggtgggc cggccgacgg gtgacgcgat cggggccgtc
     8821 gtgggctcga tgcgcgatgc ggggcggagt cgtgagatga gcgcgatcca gcgaacgctg
     8881 aagatccggc cggacgaaac gcggctcgtc ctgtcgatgt gcgccctgac gttcgtcggc
     8941 atggccggcc tcgcgatcgg gcagagcggc gcgaacgcgc tcttcttcga tcggatcggc
     9001 accgacgcgc tcccgctgat gtacctcgcg cagggcgcga ccgcgttcgt gttcatgatg
     9061 ggcctggccg cgctgctcgg acgcgtggat cggcgccgcg cctacctcgc gatcccggcg
     9121 gggctgggcg cagtcgtcct ggccgaacgg ctggcgctgc tcaccgacgg gcggtggatc
     9181 ttcccggtgc tgtggctgac cgtggcgctc gcgctcctgc tgcagaacgt cttcgtctgg
     9241 ggcatcgccg gcgtcgtcac cgacacccgt cgggccaagc gcctgttccc gctgttcgcg
     9301 gccggcgaga tcctcggctc ggtggccggc ggcctgttca cggatccgct cgtgggcgtg
     9361 atcggcacgg agaacctgct cctggtctgg gcggccgcgc tcgcgatctc gttcgcgctg
     9421 tgccgcgtgg tcctgtcctc ggccgggacg ctcgcgcaac cccgggtccg cagggccagg
     9481 cgccgggagg cgtcgccgtg gcgagaactc accgtcgcgt tcgggtacgt gcggcgctca
     9541 cgcctgctcg tgtggatgac gctcgccgcc gtgctgttct ccgtcctgtt ctactccctg
     9601 tacctgccgt gggcggccgc cgcgaccgag cggttcccgg acgcggccga cctggcgggg
     9661 ttcttcgggc tgttctgggc cgcgatgacg ggcgccgcgt tcctcgtgtc ggtgctcgtc
     9721 accaaccggc tcttcggccg gttcggcatc gcgtcgatga tgctcgtgct cccgctgctt
     9781 tacgtcgggt cgttcggcat cctgctcgtg agcgcgggat tcgtgacgct cgtgatcctg
     9841 cggttcgtcg acggggtgtg gctgcagggc atcgcctccc ccgcgtggga gacgctcacg
     9901 aacgtcgtgc ccgatgctcg acgcgatcag gtgcggacgt tcctgaacgg aggacccgcg
     9961 caggccggga ccgcgatcgc ggggatcatc gcgctggtcg gccaggacgt gctgaccccg
    10021 cggcagttcg ccgcgatcgg gctcgcgacc gccgcgatcg cggtgttcgt cacgtggcgc
    10081 gtacgcgcgt cgtacgcccc cgcgctcgtc gaggcgttgc gcgagggacg gccggaggtg
    10141 ttccccgacg tcgcggtggc cggggtcccg ttcgcgttcg cgcccgacgc ccagacgctc
    10201 ggcagcctgc tcgtcgcgat ggaggacggg cagcccactg tccggcggct ctcggtccag
    10261 ctgctcgccg acgtcgacga cccccgggcc accgcggcgc tcgaacgcgc gctgcgcgac
    10321 gacgacgcct ccgtccgcgc gcaggccctc gacggcctcg cggcaacccc cgggcgggtc
    10381 ggggccgaga cgctcctgcc actgctcgac gatcccgatc cggcggtggc cgcctccgcc
    10441 gcggcggccg tctcgacggg tacgcacacg cccgacgccg ctcgcgtgct gctccggctc
    10501 gccgcccacg ccgacccctc ggtgcgatcg accgcgctcc gacacctcgt ggcggttccg
    10561 gccgatgccg ccgccgggct tgcggcgccc gcgctcgacg acgaggactc gggggttcgg
    10621 tccgccgcgc tgacgaccct cgccgcggtg cgacccgacg aggccttggg gccggcgatc
    10681 cgcgggttcg tcgacccgag cgcgagggtg cgcgaggcgg ccgtggccgc cgcggtgcgg
    10741 atcggcgacg gctcggtgga cgctgtcctc gacgccctgg ctcgcccggc cgcccgcgac
    10801 gccgcgctgc gggcgctcgc gcgcctggac ctcggcggcc gggacgagcg cgtccgcgcg
    10861 ttcgtcgccg agcgatcgcg cgcggcgacg ggcgacgatg cgctcgccgc ggcggtccca
    10921 tcggacgggg aggcgccagg cctcctgaag gacgcgctgc tcgatcgcgc ccgggccaac
    10981 ggccgctccg cgctgcgggc gctgtcgttg atctcgagcg acggcgccgc ggtccgctcc
    11041 gcggtcgaga gcctcgacgc gcgggacccc gaacaggtcg cgaacgccct cgagacgctg
    11101 gaggcgacca ccgacgcctc gcttgccgcg tcgatcctgg cgctgtggga gccgaccggg
    11161 ggggctcgcc gcaacctgcc cgaggactgg ctggaacggg cttcggcgga cgatgatgcc
    11221 ttcgtcgcgg cgtgcgccgg ggcggtgacc gtgcagcgcg gcggagggga cgggatgact
    11281 cgcagggcga cgacgacgcc ggtgatcgag cgcgtcctgt tcctgcgtcg cgtcccgctg
    11341 ttcgaggggc tcgcacccgc cgacctgggc gcgatcgccg aggtggccga ggagcgatcg
    11401 ttcgcggacg gggagctcct cgcgtccgag ggagagctgg gcgacgagct cctgatcgtc
    11461 gcgagcggca ccgtccgcgt ggaggccggc gggtccgaga tcgctcggcg gggtcggggc
    11521 gaggtgctcg gcgagatgtc cctgatcacg cgcggtccgc gtgtcgcctc gctcgtcgcc
    11581 gacggagacg tccgcgcgat ctgcatcggg cgccgcgagt tcgagagcat gatccacgac
    11641 cgccccgaca tcgggatcgg cgtcatgcgc gtgctcgccc aacggctcgc ggaggcgcgg
    11701 ccggcagagc cgtcgccggc gtcgaacgcc tgaaggcggc tcaggcggcg gtgggcggtt
    11761 cggggatcgg agccgcgggc agcgagggcg ccgggaccga cgtcgatctg cgagcggcga
    11821 tcacgagcgc accgagaccg ccgatcgatg ccaccaccca gaccaggccg ccgaggaacg
    11881 ggatcaaccc gatcccgcga aggatcaacc accccacgag gaacgccacg taccgcgatc
    11941 ccggttggtt caccagccgc cttccgagca ggtgcgcgcc ggcgacgtag ccgagcgtgt
    12001 acagcagggc gagcgcgagc agcaggaaca ggcccagcgg gatcgcgatc accacgacga
    12061 gcagcagcac cgcggcgatc ggcagcagga agaagatcac cgcgccgaac ccgaaggagg
    12121 cgcccatccg ccggcggaac gcgtcggcga gcggcgcgtc gacgccgggg aggaacagga
    12181 ggagcacgag gccgaggatg agggtggaga tcgtgtagcc gaaccaccac acgtagcgtc
    12241 cgacccagcc gagcccctcg aggtcgaacc ggcccgagat ccccttgatc tggccccgga
    12301 ccgtggcgcc ctcctcgacc cgcgggtctt gccggctggc gatgtcgccg cccacctcgg
    12361 cgccggaacg gacgatcacg cggccgttga aggcgacgat gtcgtcaccc accgtcccgg
    12421 tgatctcgac gtcgccgttg aagaccacca cgctctcggt ggccgtgccg tcgatcgtcg
    12481 cgtcgccgtt caagatcacg gccgttccga cggtggctcc cttggggacg agcagctctc
    12541 ccgtcaggac gacctgatcg tcgtcgcccg gtgtcgcgcc ctcgtcctgg gcgaacgccg
    12601 agggcacgga cgcgatcccg atggcgcacg cgacgaacgc cacggcgagg aacgcccggt
    12661 gtgccgatcg tgctcggttc tcgcgcatgc ctgcgacctc cggccgctcc gtgtcggaat
    12721 gaccttactc gtcgcgcgcc gcgcgatgcc accggtcacg ctgcgcgccg acgcgaggat
    12781 acggttgtcg ccggcggtag acgaaatggt accgtggtcc cgagggcaac gatcgcggtt
    12841 ttccagctac gagcagttcc ctcacccggc tgctacggcc ggcgccgtga agggcagcaa
    12901 ccaggcccgg acggcgaaag aaaggaggga tcgcacgtgg caatcggaac cgtgaagtgg
    12961 ttcagccccg agaagggctt cggcttcatc actccggacg acggtaccgc cgacgtgttc
    13021 gtgcatttca gcgcgatcgc cggtgagggt ttccgcaacc tggaggagaa ccagaaggtc
    13081 gagtacgacg tgacccaggg ccagaagggc ccgcaggccg cgaacgttcg cgccgtctag
    13141 ttcgatcgaa cgcgatgagg acccggcgca ccgcgtcggg tcctctgcgt ttccgccccc
    13201 gcgagctc
//
LOCUS       DATBRK010000019         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2313185, whole genome shotgun sequence.
ACCESSION   DATBRK010000019 DATBRK010000000
VERSION     DATBRK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313185"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..677
                     /locus_tag="VIH70_00605"
     CDS             219..677
                     /locus_tag="VIH70_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114891.1"
                     /translation="MRVLLVSPDAEARDLLRISVGSIERRLGEAVSFLEAEEGERGAR
                     IGLRERPDAVVADEIASRAGAFSLARDLRGAVEPYRGPIVIVLERKHDAWLARWSGAD
                     AWLVRPVDPFELADRLVELIQAYRARPAAPPAGQPREDPTEQSREQREIA"
     gene            674..958
                     /locus_tag="VIH70_00610"
     CDS             674..958
                     /locus_tag="VIH70_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114892.1"
                     /translation="MKIKGTCRRCGREFLAEQVIRNGGHCPWDGKPFQADYSVVLVDS
                     LADAESAGNTLENALEKLADIEPDFVLDIESVLGEIRGHLERLERSHGGG"
     gene            963..1217
                     /locus_tag="VIH70_00615"
     CDS             963..1217
                     /locus_tag="VIH70_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114893.1"
                     /translation="MTSAPEVEARNRFSVEVRDGQDGQGIGQGIGWSVAILDPGGREV
                     AARACRDETEARTFASTVHQHIAWLSEARFREIYRLAEGA"
     gene            1327..1683
                     /locus_tag="VIH70_00620"
     CDS             1327..1683
                     /locus_tag="VIH70_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY8114894.1"
                     /translation="MDPETLAALDFCYVTTKGRRSGSPHTVEIWFALRGDVVYLLAGG
                     IEESDWVKNLRADPTVGLRLGDRDLICHGRLVDDPEEDALVRRLLLDKYGPRDADDLS
                     EWARTARPVAIDLPRE"
     gene            complement(1699..1992)
                     /locus_tag="VIH70_00625"
     CDS             complement(1699..1992)
                     /locus_tag="VIH70_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY8114895.1"
                     /translation="MSVHAVVLIECEIDEIPEAAQAIADLEGVSEVYSVAGEFDLVAI
                     VRVANHDDLASVIPGGISKVEGVARTETLIAFQVYSKHDLERLFSIGFDETPS"
     gene            complement(2021..>2264)
                     /locus_tag="VIH70_00630"
     CDS             complement(2021..>2264)
                     /locus_tag="VIH70_00630"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY8114896.1"
                     /translation="IEINEAFAAQVLACERELKFDRDRLNVHGGGISLGHPIGMSGAR
                     IILSLAYAMRSRAAALGLATLCISGGQGLAVVLELA"
BASE COUNT          357 a          760 c          821 g          326 t
ORIGIN      
        1 gagcgccacg ccggtcaggt cgagtccgag gctcaccgcg atcacggcgc ccgcgccgat
       61 gaagatcaac gcgaacgtcg ccacgaactc cgccaccgcc gccttgcccg tgccctccac
      121 gaccgcctcc tcgccaccga aatctcgacc cgaccggacg atagcggtgc ccggtggcgg
      181 gctcaaacga cgcagccgtg gttgatagga tgcgccgcgt gagggtcttg ctcgtgtcgc
      241 ccgacgccga ggcgcgagac ctgctgcgga tctcggtcgg gagcatcgaa cgtcgcctcg
      301 gcgaggccgt gtcgttcctg gaggcggagg agggcgaacg gggcgcccgg atcgggctcc
      361 gcgagcgccc cgacgcggtc gtcgccgacg agatcgcctc gagggcggga gcgttctcgc
      421 tcgcgcgcga cctgcgcggc gcggtcgagc cgtaccgtgg gccgatcgtg atcgtgctcg
      481 agcgcaagca cgacgcatgg ctcgcccgtt ggtcgggggc ggacgcgtgg ctcgttcgtc
      541 cggtcgaccc gttcgagctg gcggaccggc tggtcgagct gatccaggcg taccgggctc
      601 gcccggcggc accgccagcg ggacagccac gggaggatcc aacggagcag tcaagggagc
      661 aaagggagat cgcgtgaaga tcaaggggac gtgccggcga tgcggccgag agttcctcgc
      721 cgagcaggtg atccgaaacg ggggccactg cccgtgggac ggcaagccgt tccaggccga
      781 ctactcggtc gtgctggtgg attcgctcgc cgatgccgag agcgcgggga acacgcttga
      841 gaacgcgctc gagaagcttg ccgacatcga gcccgacttc gtgctcgaca tcgagtcggt
      901 gctcggggag atccgtggcc acctcgagcg gctcgaacgt tcccacggag gcgggtgagg
      961 tcgtgacctc ggcgccggag gtcgaggccc ggaaccgctt ctcggtcgag gtccgcgacg
     1021 gccaggacgg ccaagggatc ggccaaggga tcggatggag cgtggcgatc ctcgacccgg
     1081 ggggacgcga ggtcgcggcg cgggcctgcc gcgacgagac cgaggcccgg acgttcgcgt
     1141 ccaccgtcca ccaacacatc gcgtggctgt cggaggcgcg gttccgcgag atctaccggc
     1201 tcgccgaggg ggcgtgacgt gggcttcaac gcgatgcagc ccggcaggct caagcgcggc
     1261 gatctgatct tcttcgtggt cggggtcgcg gtgatcgtcg ccctgatcgt gtgggccgtc
     1321 tcgtaggtgg acccggagac cctcgcggcg ctggacttct gctacgtcac caccaaggga
     1381 cggcgttcgg ggagccccca cacggtcgag atctggttcg cgctccgggg cgacgtggtg
     1441 tacctgctcg ccggcggcat cgaggagagc gactgggtga agaacctccg cgcggatccc
     1501 accgtcggtc tgcgactcgg cgaccgggac ctgatctgtc acggacggct cgtggacgat
     1561 ccggaggagg atgccttggt ccggcggctg ctcctggaca agtacgggcc ccgcgacgcc
     1621 gacgatctgt cggagtgggc caggaccgct cgaccggtcg cgatcgacct gccccgggag
     1681 tagctaggac cgagcgagct aggacggggt ctcgtcgaag ccgatcgaga acagacgctc
     1741 caggtcgtgc ttcgagtaca cctggaacgc gatgagcgtc tcggtcctcg cgacgccctc
     1801 gaccttcgag atcccgccgg ggatcaccga cgccagatcg tcgtggttgg cgacgcgcac
     1861 gatcgcgacc aggtcgaact cgccggctac cgagtagacc tccgagacac cctccaggtc
     1921 ggcgatcgcc tgcgcggcct cggggatctc gtcgatctcg cactctatca ggacgaccgc
     1981 gtgcacgctc aacgggtcag ctcctgcctc gagggtgggc tcacgccagc tccaggacga
     2041 cggcgaggcc ctggccgccg gagatacaga gggtggccaa ccccagcgcg gccgcgcgcg
     2101 agcgcatcgc ataggcgagc gagaggatga tccgggctcc cgacatcccg atcggatggc
     2161 cgagcgagat cccgccgccg tgcacgttca gccggtcgcg gtcgaacttc agctcgcgct
     2221 cgcacgcgag cacctgcgcc gcgaacgcct cgttgatctc gatc
//
LOCUS       DATBRK010000020         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_580293, whole genome shotgun sequence.
ACCESSION   DATBRK010000020 DATBRK010000000
VERSION     DATBRK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580293"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..364)
                     /locus_tag="VIH70_00635"
     CDS             complement(53..364)
                     /locus_tag="VIH70_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114897.1"
                     /translation="MDELDFAGWNGADWEGAFARYHTDDVLVDVHGQAPTHGIREHVR
                     AMKAFVESTGGAPVQVKSHPIRFGSGDWTCVVGELEDGSRMVTVAKWRDGAIAEEYVW
                     I"
     gene            complement(416..1036)
                     /locus_tag="VIH70_00640"
     CDS             complement(416..1036)
                     /locus_tag="VIH70_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8114898.1"
                     /translation="MNEILVLPAGPLADASLAIARASESQAILDHSIRSFLFARLLAA
                     EEGCLNDAGYDEDLLFAATVMHDLGLGEHASGEARFEVEGADLAAAVLRQHGVAEPDI
                     DQVWEAIALHSSVGIADRRGLLTYLTHKGVFIDAGRITDAVADRLRQEVLTAFPRPAG
                     DRSIVDAIVAHATRSEAAAPPGSLAFELLRQHREAERARVRRQEST"
     gene            1108..2067
                     /locus_tag="VIH70_00645"
     CDS             1108..2067
                     /locus_tag="VIH70_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8114899.1"
                     /translation="MTPKRPHRVAVLGLPGLIPLELGIATEVFGRDPHYDVTVCAEGG
                     VAPGVGPGITVTTSAGLKVLREADTVIVPGYEDLDATPSAAVLDALRLAHGRGARLVS
                     ICTGAFAVAAAGLLDGRPATTHWLWTDELQRRHPLVEVLPNRLFVDDGDILTSAGMTA
                     GIDLCLHLIRRDHGAAAANTAARVLVAPPQRQGGQAQFVERLRAEVTGDQLGPLRDWM
                     LENLALPLDLDTLAGRAHMSRRTLTRRFREETGVAPMAWLTDARIDRARVLLETTTEP
                     IENIGRLTGLGAPASVRAAFHRRIGTSPKEYRAVFHHGAPTSA"
BASE COUNT          355 a          782 c          748 g          352 t
ORIGIN      
        1 gcgagctggc gctgcaagtg gagttgtgcg cagcgggcgc gggtgaggac cactagatcc
       61 agacgtactc ctcggcgatg gcgccgtcgc gccacttcgc cacggtcacc atccggctac
      121 cgtcctcgag ctcgccgacc acgcaggtcc agtctcctga tccaaaccgg atcggatgag
      181 acttgacctg gacgggcgcg ccaccggtgc tctcgacgaa ggccttcatc gcccgtacgt
      241 gttcacgaat gccgtgcgtc ggagcttgcc cgtgcacatc taccagaacg tcatcggtgt
      301 ggtagcgggc gaaggcgccc tcccagtccg ctccgttcca gcccgcgaag tcgagctcgt
      361 ccatccgttc gagattgtgg gctactgatt cgtcggccac gacggttgcc tcccttcagg
      421 tcgattcctg gcgccgcact cgggcgcgct cggcttctcg gtgttggcga aggagttcga
      481 acgcgagcga accgggaggc gcggccgctt cggaccgggt cgcgtgcgcc acgatcgcgt
      541 ccacgatgga gcggtctccg gcgggcctcg ggaaggcggt gagcacctct tgccgcaatc
      601 tgtccgcgac ggcgtccgtg atccggcctg cgtctatgaa gacccccttg tgcgtgaggt
      661 aggtcagcag accgcgacgg tcggcgatgc cgaccgacga gtggagggcg atcgcctccc
      721 agacttggtc gatgtcgggt tcggcaacgc cgtgctgcct gaggacggcg gcagccagat
      781 cggcgccctc gacctcgaag cgagcctcac ctgatgcgtg ctcaccgagt cccaagtcat
      841 gcatcacggt cgcggcgaag agcaggtcct catcgtagcc ggcgtcattc agacagccct
      901 cttcggcggc caacaagcgg gcgaacagaa agctccggat gctgtgatcg aggatcgcct
      961 gactctccga ggcgcgggcg atcgcaagac tggcatcggc gagcggcccg gcggggagca
     1021 cgaggatctc gttcatggcc gcaagcgtat ggacatcgcg agaaggtcaa cagtggcccg
     1081 agggccgttt accgctatga tcaggccatg acgcccaagc gcccgcatcg ggtggccgtg
     1141 ctcgggctcc ccggcctgat cccactggag ctcgggatcg cgacggaggt cttcggccgc
     1201 gacccgcact acgacgtgac ggtgtgcgcc gaaggtgggg tggcaccggg agtcgggccc
     1261 ggcatcaccg tcaccacatc ggctgggctc aaggttctcc gcgaggcaga caccgtgatc
     1321 gtgcccggct atgaggacct cgacgccacg ccgtcggcag cggtgcttga cgccttgcga
     1381 ctggcgcatg gtcgcggcgc ccgcctcgtc tcgatctgca caggcgcgtt cgccgtcgcc
     1441 gctgccgggt tgctggacgg acgccccgcc acgacccatt ggctgtggac cgacgagcta
     1501 cagcgtcgcc accccttggt cgaggtgctg cccaaccgcc tgttcgtcga cgacggcgac
     1561 atcctcacgt ccgccggcat gacggccggc atcgacctgt gcctccacct catccggcgc
     1621 gaccacggcg ccgccgcggc caacacggcg gcacgggtcc tcgtcgcgcc gccacaacgt
     1681 cagggaggac aggcgcagtt cgtcgaacgg ctgcgcgccg aggtcaccgg cgatcagctc
     1741 gggccgcttc gcgactggat gctggagaac cttgcccttc cgctcgacct tgacacgctc
     1801 gcggggcgcg cacacatgtc tcgacgcacg ctcactcgtc ggttccgcga ggagaccggg
     1861 gtcgctccga tggcctggct caccgatgcg aggatcgacc gagcacgcgt gctgctggag
     1921 acgaccaccg agccgatcga gaacatcggc cgcctcacgg gactgggcgc gccggcctcg
     1981 gtgcgagcgg ccttccaccg gcgcatcggc acctcgccga aggaataccg ggcagtcttc
     2041 caccacggcg cacccacgtc ggcgtgatca acacccaagc accgagtcag caacgccgca
     2101 tcggctagtg acgcggtccg gccggccaac agggttgagc agaccgtccc tatcggggcg
     2161 gtgaatgcct ggcgcccgcc ctcaaccctg tacacctctt gaccggacgc ggccggcgtg
     2221 ggacctcgcg ctggatg
//
LOCUS       DATBRK010000021         5404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1735142, whole genome shotgun sequence.
ACCESSION   DATBRK010000021 DATBRK010000000
VERSION     DATBRK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735142"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="VIH70_00650"
     CDS             complement(<1..1977)
                     /locus_tag="VIH70_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY8114900.1"
                     /translation="MLESLVPVRDRVSFSKLDEALPLPDLVGIQRESFDWLLAEGLKE
                     VLEEVSPIEDFTEQFQLHFGKHAFKDQKFTEEECKDKDATYSAPLFVEAMFVNKTTGE
                     IKEQEVFMGDFPMMTERGTFIINGTERVVVSQLVRSPGVYFSRELDKTTDKDVYIAKI
                     IPSRGAWLEFDVDKKDTVGVRIDRKRRQNVTVLLKALGWSGDEILGLFDHAESIKNTL
                     EKDHVETPEEALEDIYRKLRPGEPPTAESARTLLENLFFNHKRYDLARVGRYKVNKKL
                     EAAQGVLQTQLKARARQLAELDNPDKKGWDQPKFRVWSELQKDEQGENGPKYKGILSY
                     EDMLRTVRYLVKLHAGEEGFEPDDIDHFGNRRLRSVGELIQNQIRIGLSRMERVVRER
                     MTTQDVEAITPQTLINIRPVVASIKEFFGTSQLSQFMDQTNPLAGLTHKRRLSALGPG
                     GLSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLIGSLSTYGRINQFGFIETPYRRV
                     TGGKVTDKVDYLSADEEDRHVIAQANEPIKPDGAYANPTVLVRRKGGEVDSVVPEEVD
                     YMDVSPKQIISVAAALIPFLEHDDANRALMGANMQRQAVPLLRSEAPIVGTGVEFRAA
                     VDAGDVVLAEEAGVVEDVSADDIVIRERAGSMKTY"
     gene            complement(2164..2583)
                     /locus_tag="VIH70_00655"
     CDS             complement(2164..2583)
                     /locus_tag="VIH70_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8114901.1"
                     /translation="MTGERTFGQRLRATRIKAGYSQSDLEEISGIPKARLSRYENGHV
                     APSIQTLERLARALNVSEASLLGDDRALMEEFFHVLTTRGVRIGSSEQAVALGSAVAD
                     IARALGMVDEGEGAVADRSTLSPVGVAGTAAWSPDGS"
     gene            complement(2710..3105)
                     /gene="rplL"
                     /locus_tag="VIH70_00660"
     CDS             complement(2710..3105)
                     /gene="rplL"
                     /locus_tag="VIH70_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY8114902.1"
                     /translation="MAKTKMTADDLLDQFKDMTLLELSDFVKKFEETFDVQAAAAAPM
                     MMAAPGTAGGAAEEVEEQTEFDVVLTGAGDKKIQVIKEVRALTSLGLKEAKDLVDSAP
                     KPVLEKVNKEAADKAKEQLEGAGAAVEIK"
     gene            complement(3109..3804)
                     /gene="rplJ"
                     /locus_tag="VIH70_00665"
     CDS             complement(3109..3804)
                     /gene="rplJ"
                     /locus_tag="VIH70_00665"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY8114903.1"
                     /translation="MPNAEKIAKVAELKERIQGAEALLLTEYRGLSVHDATELRRALS
                     ELARFAIVKNTLMARAAADAGVAELEALLDGPTAVAFVEGDVVSTAKKVVDAAKRFPA
                     LVIKGGYLDGKVLSATEAQSLATLESREAMLSKVAGMLKAELTRAASMLEAIQGRFLG
                     VLEAYKEKLPSEVTDETAEAEPAAEPEPAEGSEPAEGSEPAADATTDDEQDVRPQGEP
                     EGESAEEPEGEEE"
     gene            complement(4024..>5404)
                     /locus_tag="VIH70_00670"
     CDS             complement(4024..>5404)
                     /locus_tag="VIH70_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114904.1"
                     /translation="FGAKRILAWFASGELFDRGRPPMITILVAVGILVALWRYRRDER
                     ARVLLFLFLVSLVLFFGRPTLGPVLRLLPGSGDLFLRRFVSGVHLAGLYLAGFGAVTL
                     GRIAWNQLQRFRALRERPAAIASLAVACLVLVLAPGFIERTAWASQGAEWIGEQAVAD
                     DQGGADVAALVDIARARGPGRFYAGMRSNWGSAYEVGQVPLYVVLLNQGTEGVGFVRP
                     TWSLSAPFEYRFTDTNPSHYDLFDVRYLILEAGKPPPVQAERVAERGRHVLWERPDPG
                     AIDLVDVLPAITADRTNLGLRGEDWFRSDLPAMGANPGIAFEGEPAPEATVSAEDLPD
                     TPAGIVVHEDVDLRDGRASATVIAYRPAMVMLKASFDPRWQVTVDGTALAPEMIAPSF
                     VGRTVPAGRHVVRFVYEPFPRYDILLTLGALTLLGLGLLPGRFARHGSGGSGHGAHDP
                     GAETSAAGT"
BASE COUNT          803 a         1902 c         1791 g          908 t
ORIGIN      
        1 gtaggtcttc atcgagccgg cccgctcgcg gatcacgatg tcgtcggccg agacgtcctc
       61 cacgaccccg gcctcctcgg ccaggaccac gtctccggcg tcgacggcgg cgcggaactc
      121 cacgccggtt ccgacgatcg gggcctccga ccggagtagg ggcacggcct gccgttgcat
      181 gttggccccc atcagggccc ggttggcgtc gtcgtgctcg aggaagggga tcagggccgc
      241 cgcgaccgag atgatctgct tgggcgagac gtccatgtag tcgacctctt cgggcacgac
      301 cgagtcgacc tcgccgccct tgcggcggac caggacggtc gggttggcgt aggcgccgtc
      361 gggcttgatg ggctcgttgg cctgcgcgat gacgtgccgg tcctcctcgt cggccgagag
      421 atagtccacc ttgtcggtga ccttgccgcc cgtcacccgg cgatacgggg tctcgatgaa
      481 cccgaactgg ttgatgcgtc cgtaggtcga gagcgagccg atcaggccga tgttcggccc
      541 ctcgggggtc tcgatcgggc acatccgtcc gtagtgcgag gggtgcacgt cccggacctc
      601 gaaccccgcg cgttcgcgcg agagcccgcc cggccccagc gccgacagcc ggcgcttgtg
      661 ggtcagcccg gcgagcgggt tggtctggtc catgaactgc gacagctgcg aggttccgaa
      721 gaattccttg atgctcgcga ccaccggtcg gatgttgatc agcgtctggg gcgtgatcgc
      781 ctcgacgtcc tgcgtcgtca tccgctcgcg gacgacccgc tccatgcgcg acagaccgat
      841 ccggatctgg ttctggatca gctcgcccac agagcgcagc cgccggttcc cgaagtggtc
      901 gatgtcgtcg ggctcgaagc cctcctcgcc cgcgtgcagc ttcacgaggt atcgcaccgt
      961 gcggagcatg tcctcgtagg acaggatccc cttgtacttc ggcccgttct cgccctgctc
     1021 gtccttctgg agctccgacc agacgcggaa cttcggctgg tcccacccct tcttgtcggg
     1081 gttgtcgagc tccgccagct gccgggcgcg ggccttcagc tgcgtctgaa gcacgccctg
     1141 ggcggcctcg agcttcttgt tgaccttgta ccgacccacc cgagccaggt cgtagcgctt
     1201 gtggttgaag aagaggttct ccaggagcgt ccgggcggac tcggccgtcg gtggctcacc
     1261 gggccgcagc ttgcggtaga tgtcctccag ggcctcctcg ggggtctcga cgtggtcctt
     1321 ctcgagggtg ttcttgatcg actcggcgtg gtcgaagagc ccgaggatct cgtcccccga
     1381 ccacccgagc gccttgagca gcaccgtgac gttctgccgg cgcttgcggt cgatccggac
     1441 accgacggtg tccttcttgt cgacgtcgaa ctcgagccat gcccctcggc tggggatgat
     1501 cttggcgatg tagacgtcct tgtcggtggt cttgtccagc tcgcggctga agtagacccc
     1561 cggggagcgc acgagctggg acacgaccac ccgctcggtg ccgttgatga tgaaggtgcc
     1621 gcgctcggtc atcatgggaa agtcccccat gaagacctcc tgctccttga tctcgccggt
     1681 cgtcttgttg acgaacatcg cctcgacgaa cagcggagcg gagtaggtgg cgtccttgtc
     1741 cttgcactcc tcctcggtga acttctgatc cttgaaggcg tgcttcccga aatgcagttg
     1801 gaactgttcg gtgaagtcct cgatcggcga gacctcctcg aggacctcct tcagaccctc
     1861 ggccaggagc caatcgaagg actcccgctg gatgccgacg aggtcgggaa gcggtagcgc
     1921 ctcgtcgagc ttggagaaac tgacgcggtc gcggacaggg acgagcgact caagcaagaa
     1981 cgatcccccc tagggtagac aaccctggat ccagggcatg cggaacgcgg cacggcaaac
     2041 aagcagtcta cgtgcgcggg cgcgcagcgt caacccccgg gcggggaccc ggtccaaatc
     2101 ggcccgaagg ttacccccac tcggagcgga ggtcaaccct tcggaaccgg tcgtcgaccc
     2161 ggcctagctg ccgtcgggtg accaggcggc ggtacccgcc acaccgaccg gcgagagggt
     2221 cgaccggtcc gcgacggcgc cctccccctc gtccaccatc ccgagcgcac gcgcgatgtc
     2281 ggcgacggcg ctcccgagcg ccacggcctg ctcgctcgag ccgatccgga cgccgcgagt
     2341 ggtgaggacg tggaagaact cctccatcag ggcacggtcg tcccccagga gcgaagcttc
     2401 cgacacattc agcgcccggg ccaggcgctc gagtgtctgg atcgaggggg cgacgtggcc
     2461 gttctcgtag cgcgacaggc gcgccttcgg gatcccggag atctcctcca ggtccgactg
     2521 cgagtagccc gccttgatgc gcgtcgcgcg gagcctctgt ccgaacgtgc gttcgccggt
     2581 catgccacgc cccctgtcga tggtggttgc tcgacgagga atctatccct atgcggagcc
     2641 cgcccgcaac gcggccgaga aagccggcgc ttggcaacct cggcgatccg gccggctcgg
     2701 ccgttcggcc tacttgatct cgacggccgc gccggcgccc tcgagctgct ccttggcctt
     2761 gtcggccgcc tccttgttga ccttctccag aaccggtttc ggcgcggagt ccacgaggtc
     2821 cttggcctcc ttgaggccga ggctcgtcag ggcccgcacc tccttgatga cctggatctt
     2881 cttgtcgccg gcgcccgtga gaacgacgtc gaactcggtc tgctcctcga cctcttccgc
     2941 ggcgccaccg gcggttcccg gggcggccat catcatcggc gcggcggcgg cagcctggac
     3001 gtcgaaggtc tcctcgaact tcttgacgaa gtccgacagc tcgagcagcg tcatgtcctt
     3061 gaactgatcg agcaggtcgt cggcggtcat cttggtcttg gccatcgtct actcctcctc
     3121 cccttctggc tcttctgctg actccccttc gggctctcct tgcggccgga cgtcttgttc
     3181 gtcgtcggtc gtcgcgtcgg cggcgggctc ggatccctcg gccggctctg atccctcggc
     3241 cggctcgggc tccgcggccg gctcggcctc cgcggtctcg tcggtcacct cgctggggag
     3301 cttctccttg taggcctcga ggacgccgag gaagcgaccc tggatcgcct cgagcatcga
     3361 ggccgcccgg gtcagctcgg ccttcagcat cccggcgacc ttcgacagca tcgcctcccg
     3421 cgactcgagg gtcgcgagcg attgggcctc ggtcgccgag agcaccttgc cgtccaggta
     3481 gccgcccttg atcacgaggg ccgggaacct cttcgcggcg tccacgacct tcttggcggt
     3541 cgagaccacg tcgccttcga cgaacgcgac cgcggtcggg ccgtccagga gcgcctccag
     3601 ctcggcgacg ccagcatcgg cggccgctcg cgccatgagc gtgttcttga cgatcgcgaa
     3661 ccgcgccagc tctgaaagcg cccggcgcag ctcggttgcg tcgtggacgg agagaccccg
     3721 gtactccgtc aggagcagcg cctcggcgcc ctggatgcgc tccttgagct cggcgacctt
     3781 cgcgatcttc tcggcgttcg gcatcgaccc tccatggaac gggaaacgcg tccccgcaga
     3841 cagcggagac gcgctcgaga gcgatacgtc ttcgttcgcc tcggcaggtg cccttttggg
     3901 attacgtgct tgcgcacccc tgctgtctgc ggcgaagcca aagggtaccg agccgtcggc
     3961 ttcggcgaca acgtgtcacc gacaagaaag ccaacctcgc gatcacacgt ccgcagggat
     4021 cccttaggtc cccgcggcac tcgtctcggc gccggggtcg tgcgcgccgt ggccggaccc
     4081 accagatccg tgtcgcgcga accggccggg gagcagaccg aggcccagca gcgtcagcgc
     4141 cccgagggtc agcagaatgt cgtacctcgg gaacggctcg tacacgaatc ggaccacgtg
     4201 tcggccggcc ggcacggtcc gcccgacgaa gctgggggcg atcatctcgg gcgccaacgc
     4261 cgtgccgtcc acggtcactt gccaacgcgg gtcgaacgac gctttcagca tcaccatggc
     4321 cggccgatag gcgatcaccg tcgcgctggc cctgccgtcc cgaaggtcga cgtcttcgtg
     4381 gacgacgatg ccggccggcg tgtccgggag gtcctcggcg ctcaccgtgg cctcgggcgc
     4441 aggctccccc tcgaaagcga tgccggggtt cgcgcccatg gccgggagat ccgaacggaa
     4501 ccagtcctcc ccgcgcagcc cgaggttcgt cctgtcggcc gtgatcgccg gcaggacgtc
     4561 gacgagatcg atggcgccgg ggtccggtcg ttcccagagc acgtgtcgcc cgcgttcggc
     4621 gaccctctcc gcctggacgg ggggcggctt gcccgcctcg aggatcaggt accggacgtc
     4681 gaataggtcg tagtgcgacg ggttcgtgtc ggtgaaccgg tactcgaacg gtgccgagag
     4741 cgaccaggtc ggccggacga accccacgcc ctcggtgccc tggttcagga gcaccacgta
     4801 caggggcacc tgaccgacct cgtaggccga gccccagttg gaccgcatgc ccgcgtagaa
     4861 ccgacccggc ccccgtgctc tcgcgatgtc gaccagggcg gcgacgtcgg cgccgccctg
     4921 gtcgtcggcg accgcctgct ccccgatcca ttcggccccc tgcgaggccc acgccgtgcg
     4981 ttcgatgaag ccgggggcga ggacgagcac cagacaggcg accgccagcg acgcgatcgc
     5041 cgcgggtcgt tcgcgcaggg ctcggaaccg ttgcagctgg ttccatgcga tccgcccgag
     5101 cgtgaccgcg ccgaacccgg cgaggtacaa cccggccaga tgcaccccgg acacgaaccg
     5161 ccgaaggaac aggtcgccgg agccgggcag cagccgcagg acgggtccga gggtcggccg
     5221 tccgaagaac aggaccagcg agacgaggaa caggaacagg agcacgcggg cccgctcgtc
     5281 gcgacggtac cgccagagtg ccaccaggat gccgaccgcg accaggatcg tgatcatcgg
     5341 cggtcgccca cggtcgaaga gctcgccgct cgcgaaccac gccaggatcc gcttcgcccc
     5401 gaac
//
LOCUS       DATBRK010000022         4705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_147805, whole genome shotgun sequence.
ACCESSION   DATBRK010000022 DATBRK010000000
VERSION     DATBRK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147805"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VIH70_00675"
     CDS             complement(<1..330)
                     /locus_tag="VIH70_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY8114905.1"
                     /translation="MRAPAVAVVGRQNVGKSTLVNRLFGSREAIAHEMPGVTRDRVEL
                     EATWRGRTLKLIDTGGFAHGATGIEALVSEQAERAARDADVVLLVVDVQTGPVEEDAA
                     LAGRLRRA"
     gene            complement(327..977)
                     /gene="cmk"
                     /locus_tag="VIH70_00680"
     CDS             complement(327..977)
                     /gene="cmk"
                     /locus_tag="VIH70_00680"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY8114906.1"
                     /translation="MTTVIAIDGAAGVGKSTLAHRMAAELGLPYVNTGLMYRALALRA
                     HREGVSPEDAPALERLARGMRFELDSSVRPPELKIDGRAPEAALTSAEVESSVSAVAR
                     HPEVREVLREEQRRLAVGGAVMEGRDIGSVVAPNADLKLFLRAQADERAGRRALERDR
                     EPEHIARALGARDRQDARTNPFVPAPDAIVLDTTELSPDEVANQALALVRERGLAR"
     gene            complement(974..2341)
                     /gene="aroA"
                     /locus_tag="VIH70_00685"
     CDS             complement(974..2341)
                     /gene="aroA"
                     /locus_tag="VIH70_00685"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY8114907.1"
                     /translation="MRAIVHPGAFGGGVASVPGDKSIAHRWLILGATALGGCELRWLP
                     EALDVRATAACLAALVDDDARRVLDGWASNPIPQADRDRSTANDSRPRGRDLVLGGNG
                     RAALHPTKDPLDCSNSGTTMRLLAGLLAAAPFETVLRGDASLTARPMERVADPLRAMG
                     AEVWTTDGHPPVVVRGGPLRGIEHRTVVPSAHVKSAVLLAGLAAEGSTTLREPVPTRD
                     HTERALAALGAPIEFEPGLATVRAFEHEGFAATVPGDVSSAAFLVAAAVVTGGALAIR
                     DVGLNPTRTRFVRVLERMGASVRSRIERVELGEPVGTLEVEPGPRLAGTTIDAGELPQ
                     VIDEVPVLAMVAAHASGQTRFEGGAELRVKESDRLGGLVEAIRALGGDADLRGDDLIV
                     GGSGLVGGEAQAHGDHRMAMALAVSALAARGPVAVDGIEAAEVSFPGFEELLVSLGAR
                     IEVSA"
     gene            complement(2346..3440)
                     /locus_tag="VIH70_00690"
     CDS             complement(2346..3440)
                     /locus_tag="VIH70_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEY8114908.1"
                     /translation="MTSAERVAVVGTGLIGTSIAMASVRAGETVRGFDADPDALARAA
                     ERSGLTPSATLEESVTGATLVFVCTPIPELSGLVSEILRLAPDAIVSDVGSVKTRVMS
                     GVETSADPAHVDRYIGGHPMGGSERSGPDHASASVLDGVVWVLSPAPAVPETRVARLA
                     AWVEKVGARPVRMDPERHDRLVAIVSHLPQVASTALMGLAASEEAGEPEILLLAAGGF
                     RDLTRLAASSPHLWSDILLANGDAIVRAIDLYVERLTRLRTLIDRADAAEVERAFGEA
                     KRARLSLAAKPQVKAGVAVLQVPVPDRPGVLAELTATMSDAGVNIEDLQIVHSPEGGR
                     GTVHLTLAADAARDAELALRERWFEPQRLA"
     gene            complement(3427..3795)
                     /gene="aroH"
                     /locus_tag="VIH70_00695"
     CDS             complement(3427..3795)
                     /gene="aroH"
                     /locus_tag="VIH70_00695"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY8114909.1"
                     /translation="MGEVRVRAARGAIRVDEDSSDSVLSATERLLAQMLERNEVAADD
                     IVSAFFTVTDDLRSAFPAEAARRMGLGGVPLLCAQEIPVAGSMRGVIRVLLQFHTARE
                     PAEVNHVYLDGAETLRDDLG"
     gene            complement(3875..4630)
                     /locus_tag="VIH70_00700"
     CDS             complement(3875..4630)
                     /locus_tag="VIH70_00700"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8114910.1"
                     /translation="MPVERLQRSLARAGFGSRRACEQVIAAGRVEINGRVATLGDRLD
                     PAVDEVRVDGSKVNVDPELRTFALHKPRGVTTTMRDPHAERDLGGFLPKGVHVFPVGR
                     LDRETEGLLLLTNDGSLAHRLTHPRYAIEKEYLAEVGKPPSRRQLARLRRGVELDDGT
                     ARAIDARSAGGWGGTGGRGGQGGAAGRGGVRLVMVEGRKHEVRRMLDAVELPVRRLVR
                     VRVGPIRLGKLRPGEIRELGAEDVRALYRAAGL"
BASE COUNT          634 a         1834 c         1629 g          608 t
ORIGIN      
        1 cgcgcgccgg agccggccgg ccagcgcggc gtcctcctcg acgggacccg tctggacgtc
       61 gaccaccagg agcacgacgt cggcatcgcg cgccgcccgt tcggcctgct ccgagacgag
      121 cgcctcgatc cccgtcgcgc cgtgcgcgaa cccacccgtg tcgatgagct tgagggttcg
      181 acctcgccac gtggcctcga gctctacccg atcccgggtg acccccggca tctcgtgcgc
      241 gatcgcctcc cgggagccga acagccggtt cacgagggtc gacttgccca cgttctggcg
      301 accgaccacg gcgacggcgg gcgccctcac ctcgcgagcc cgcgctcgcg gaccagcgcg
      361 agcgcctggt tggcgacctc gtccggcgac agctcggtcg tgtcgagcac gatcgcgtcg
      421 ggggccggca cgaacgggtt cgtccgcgca tcctgtcggt cccgtgcgcc gagcgcccgg
      481 gcgatgtgct cgggttctct gtcgcgttcg agcgccctcc ggccggcccg ttcgtcggcc
      541 tgcgcccgca ggaacagctt gaggtcggcg ttcggcgcca cgaccgagcc gatgtcccgc
      601 ccctccatca ccgcgccacc aacggcgagg cgccgttgct cctcgcggag cacctcgcgg
      661 acctcggggt gacgcgcgac cgccgacacg ctcgactcga cctcggcgga cgtgagggcg
      721 gcctcgggag cccgaccgtc gatcttgagc tcgggcggtc gaacgctcga gtcgagctcg
      781 aagcgcatgc cgcgcgcgag gcgttcgagc gcaggtgcgt cctccgggga gacgccctcg
      841 cggtgggcac gcagggcgag ggcccggtac atcagccccg tgttcacgta cggcaggccg
      901 agctcggccg ccatccgatg cgcgagcgtg ctcttcccga ccccagcggc gccgtcgatc
      961 gcgatcaccg tcgtcacgcc gagacctcga tcctcgcgcc gagcgagacg aggagttcct
     1021 cgaacccggg gaaggagacc tcggcggcct cgatcccgtc gaccgcgacc ggaccccgcg
     1081 ccgcgagggc ggacacggca agggccatcg ccatccgatg atcgccgtgc gcctgcgcct
     1141 cgccccccac gagcccggaa ccgccgacga tcaggtcgtc gccccgcaga tcggcgtctc
     1201 ccccgagcgc ccggatcgcc tccacgagcc cgccgagccg atcgctctcc ttgacccgga
     1261 gctcggcgcc tccctcgaac cgcgtctggc cgctcgcgtg cgctgccacc atcgccagca
     1321 ccggcacttc gtcgatcacc tgcggaagct ctcccgcatc gatcgtcgtc ccggcgagcc
     1381 gcggccccgg ctcgacctcg agggtcccca ccggttcgcc caactccacc cgctcgatcc
     1441 gggatcggac cgacgcaccc atgcgttcga ggacgcggac gaaacgcgtc cgggtcgggt
     1501 tgagcccgac atcccggatc gccaaggctc ctccggtgac caccgcggcc gcgacgagga
     1561 acgcggccga cgagacatcg ccgggcaccg tcgcggcgaa cccctcgtgc tcgaacgcgc
     1621 ggaccgtcgc caggccgggc tcgaactcga tcggggcgcc gagcgccgcc aacgcgcgtt
     1681 ccgtgtggtc gcgggtgggg acgggctccc gcagggtggt cgaaccctcg gcggccaggc
     1741 ccgcgagcag gacggcgctc ttcacgtggg cgctcgggac gaccgtccgg tgctcgatcc
     1801 ctcgaagggg cccgccgcgc acgacgaccg gcgggtggcc gtcggtggtc cacacctcgg
     1861 cgcccatcgc ccgcagcgga tcggcgacac gctccatcgg ccgtgcggtc aggcttgcgt
     1921 ctccgcgtag cacggtctcg aacggcgccg ccgccagcag gccggccagc agtcgcatcg
     1981 tcgtgcccga gttcgagcag tccagcgggt ccttcgtggg gtgaagcgca gctcgaccat
     2041 tccctccgag cacgaggtct cgacctcgag gtcgagagtc gttcgccgtg gaacggtcac
     2101 ggtccgcctg tgggatggga tttgaagccc aaccgtcgag tacacgtcga gcgtcgtcgt
     2161 ccacgagcgc ggccagacag gccgccgtcg cccgcacgtc gagggcctcg gggagccagc
     2221 ggagctcgca cccgccgagc gcggtggccc cgaggatcag ccaccggtga gcgatcgact
     2281 tgtccccggg cacggaggcg accccgccgc cgaacgcacc cgggtggacg atcgcgcgca
     2341 ccggctcacg ccagtcgctg gggctcgaac cagcgctcgc gcagggcgag ctcggcgtct
     2401 cgggccgcgt cggcggccag cgtgaggtgc accgtccccc ggccgccctc gggcgagtgc
     2461 acgatctgca ggtcctcgat gttcacgccg gcgtcgctca tcgtcgcggt cagctcggcc
     2521 aggacgcccg ggcggtcggg caccgggacc tggaggaccg ccacccccgc cttgacctgc
     2581 ggcttggccg cgagcgagag gcgtgcgcgc ttggcctcgc cgaacgcgcg ctcgacctcg
     2641 gccgcgtcgg cccggtcgat cagcgttcgc agccgggtga ggcgctcgac gtacaggtcg
     2701 atcgcccgca cgatcgcgtc gccgttggcg agcaggatgt cgctccacag gtgcgggctc
     2761 gaggcagcga gccgcgtgag gtcgcggaac ccgccggcgg cgagcaacag gatctcgggc
     2821 tcccccgcct cctcggaggc cgcaaggccc atcagggcgg tcgaggccac ctgcggcaga
     2881 tggctcacga tcgccaccag gcggtcgtgg cgttccgggt ccatccgcac ggggcgcgcg
     2941 ccgaccttct ccacccaggc tgccaggcga gccaccctgg tctccggcac cgccggagcc
     3001 ggcgagagca cccacaccac cccgtccagc accgacgccg aggcgtgatc gggccccgac
     3061 cgctccgaac cgcccatcgg atggcccccg atgtaccggt cgacgtgagc cgggtccgcg
     3121 ctcgtctcga ccccggacat cacccgggtc ttcacgctcc cgacgtccga cacgatcgcg
     3181 tccggggcga gccgcaggat ctcggagacc aggccggaca actccgggat cggggtgcac
     3241 acgaacacga gcgtcgcccc ggtgacgctc tcttccaggg tcgccgacgg ggtgagcccc
     3301 gagcgttcgg cggcccgggc gagcgcgtcc ggatcggcgt cgaacccccg gaccgtctcc
     3361 ccagccctga ccgacgccat cgcgatcgag gtgccgatca gccccgtccc gacgaccgcg
     3421 acgcgttcag ccgaggtcat cgcgcagcgt ctccgccccg tcgaggtaca cgtggttgac
     3481 ctcggcgggc tcgcgcgccg tatggaactg caggaggacg cggatcacac cgcgcatcga
     3541 ccccgcgacg gggatctcct gcgcgcagag cagggggacg ccgccgagac ccatccgtcg
     3601 cgccgcctct gccgggaagg ccgagcgcag gtcgtcggtg accgtgaaga acgcgctcac
     3661 gatgtcgtcc gcggcgacct cgttgcgctc gagcatctgg gccagcagcc gctcggtggc
     3721 cgagagcacc gagtcggacg aatcctcgtc gacgcggatc gccccgcgcg ccgctcgaac
     3781 cctgacctcg cccaccgcgc ctccacccca ccgatgtcgg cgaaaggcta caggccgggc
     3841 tcaccggcgg tcacgaacgg ctgccgtcga cggttcacag accggcggcg cggtagagcg
     3901 cccggacgtc ctcggctccg agctcgcgga tctctccggg tcggagcttg cccaggcgga
     3961 tcggcccgac gcgaacgcgg acgaggcggc gcaccgggag ctcgacggcg tccagcatcc
     4021 ggcggacctc gtgcttgcgt ccctcgacca tcacgaggcg gacccctccc cgtccggcgg
     4081 cgcctccctg tccgccccgc ccacccgttc cgccccatcc acccgccgaa cgtgcgtcga
     4141 tcgcccgtgc ggtcccgtcg tcgagctcga cccctcggcg caggcgcgcg agctggcgcc
     4201 gggacggggg cttgcccacc tcggccaggt actccttctc gatcgcgtac cgcgggtgcg
     4261 tgagccggtg cgcgagcgag ccgtcgttgg tcaggagcag cagcccctcg gtctcccggt
     4321 cgagccgacc gaccgggaac acgtgcacgc ccttggggag gaagccgccg aggtcgcgtt
     4381 cggcgtgggg gtcgcgcatc gtcgtcgtga cgccgcgcgg cttgtgcaac gcgaacgtgc
     4441 gcagctcggg gtcgacgttg actttcgaac cgtcgacccg cacctcgtcg accgcgggat
     4501 cgagccggtc gcccagcgtg gcgacccgcc cgttgatctc gacccggccg gcggcgatca
     4561 cctgctcgca cgcgcgtcgc gacccgaacc cggcgcgggc gagcgaacgc tggagccgct
     4621 cgacgggcat ccctcgtcag tcggtgtctg ccgtctcggc ctccgcgtcg cccgcacccg
     4681 cgcgcaggag cggcgcgagc gcagg
//
LOCUS       DATBRK010000023         5352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1590786, whole genome shotgun sequence.
ACCESSION   DATBRK010000023 DATBRK010000000
VERSION     DATBRK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590786"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1365
                     /locus_tag="VIH70_00705"
     CDS             151..1365
                     /locus_tag="VIH70_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8114911.1"
                     /translation="MDSLFRNEQGRAVATLIRVLGDFDLAEDAVQEAFAQAMRVWPER
                     GIPDNPGAWITTTARNRAIDRLRRERALREKTAQLGVVEALESLGGDETDVSGIPDDR
                     LRLIFTCCHPALRMDARVALTLKTLGGLTTGEIAHAFLVPEPTMAQRVVRAKRKIRQA
                     AIPYRVPPAELLPERVPGVLAVLYLVFNEGYAPTTGPLVRTDLCDEAIRLARLVVTLM
                     PDEPEIRGLLALMLLQHSRREARTDALGAMILLEDQDRARWDHAMIDEGLATLDGALA
                     ARRPGPYQLQGAIAALHARAPRPEDTDWPQIAALYDRLVETSPSPVVELNRAVAIAMA
                     DGPERGLALFAPLEVELGEYHPFHAARADLFRRVRRNEEAAAAYRRALELVTNPRQRA
                     YLEGRLAEVTGP"
     gene            complement(1352..1789)
                     /locus_tag="VIH70_00710"
     CDS             complement(1352..1789)
                     /locus_tag="VIH70_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8114912.1"
                     /translation="MWNSGEYLRRLLAQGRGELVEAVAWFGDLPGGRGMVLFPEHEEF
                     STSAVREGCAEVRDLFVARTRRRLGVATMLMETLEEAVRDRGMTRVGLSVSLDEEGAP
                     ARALYERLGYVHAHGPFVSSTDLLHDDGPVAVGAVMTYLVKDL"
     gene            complement(1900..3375)
                     /gene="zwf"
                     /locus_tag="VIH70_00715"
     CDS             complement(1900..3375)
                     /gene="zwf"
                     /locus_tag="VIH70_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010314638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY8114913.1"
                     /translation="MARELLPQAAVIFGASGDLTKRKLLPAFWHLWLQRRLPKGFALV
                     GYARSAITSEEFRQSAFDHVSKFSKHPPEGEAWEEFARRLSYHQGEFSEPGAMNDFAT
                     YLEKIDAECGTQGRRIFDAAVPPTVYADIVRRIGEAGLERDAKIVLEKPFGRDLQSAR
                     DLNEVIHSVFDEMQVFRIDHYLGKETVQNILAFRFANGMFEPIWNRRYIDHVQITVAE
                     KIGIEGRVAFYEEFGNIRDMISTHLFQVMTFVAMEPPVSFAPDRLRDETVKALRSTDI
                     SSPDRVIRGQYEGYRDEHGVAPDSTTETFSAMELDIDNWRWAGVPFFLRTGKGLAEKM
                     SEITLKFRKVPFNVFRGSDMDLPMRDHLTVRVQPNEGITIGFNVKQPGPGMHLGRAAM
                     DFDYDEAFAHTEISDAYELLLLEAMRGDHSLFIRQDGVERAWEILQPALEHPTPVCFY
                     ERGSWGPEEAEELIRPRKWHMSGVLDAHDSMGRALPTSNEG"
     gene            complement(3375..3779)
                     /locus_tag="VIH70_00720"
     CDS             complement(3375..3779)
                     /locus_tag="VIH70_00720"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8114914.1"
                     /translation="MRDGTAERSDVHIGDRSVGEDGRVDESYRRVGSRAEVPEGELRS
                     FDLPGLRVAIAHVENALYAFGDECTHEGCSLAEGTLGDQEDTVVCPCHGSEFDLATGE
                     PVGGPAVDPVPVYAVRVTDEWIEVRPPSEGGW"
     gene            complement(3772..4536)
                     /locus_tag="VIH70_00725"
     CDS             complement(3772..4536)
                     /locus_tag="VIH70_00725"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY8114915.1"
                     /translation="MSGTQPEPPSETTRIRDMPAPRVAEGVAAKPMRWAFTWPYRVIL
                     LGVYRAGFRPWHLTVLGLVTNVWVGASLLRGQFLLAGLLLIPAGLFDILDGAVARLRG
                     EDSRWGAFLDSVLDRVADVILFGCLFWSLAGQGLRLEAALALLTLVIALCVSHVRAEA
                     EAAGVELTEGYFQRLERYIAMMIGLPIPGALLPALAILTVLGAVTLAQRVWSAVTRVL
                     AVSSGGGVDEVAVREQGPPVEPTRETASDPNATLGA"
     gene            complement(4597..5130)
                     /locus_tag="VIH70_00730"
     CDS             complement(4597..5130)
                     /locus_tag="VIH70_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEY8114916.1"
                     /translation="MELPEELANRPPRKGGQLPTSTLTLDAYLRLRGELDDLKISGRV
                     AIAERLKAAREHGDIRENAEYDAAKNEQALMESRIRNLERMLRDPEIVEGPAETEVVV
                     SGMLVTLRPLEEDEPEDEIYLLAESAEERESGVRTITTTSPLGKALLGANLEDQIAYE
                     APGGTFHYRVVAFEPRT"
     gene            complement(5193..5336)
                     /locus_tag="VIH70_00735"
     CDS             complement(5193..5336)
                     /locus_tag="VIH70_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8114917.1"
                     /translation="MISVNSAHSVHTEAPFGGYKQSGIGREMGMHAVNLYTEVKNVFF
                     AES"
BASE COUNT          805 a         1941 c         1804 g          802 t
ORIGIN      
        1 ctacctggtc gactgcccga gcctggacga ggcgatcgag gcgagcgcga agatgccggt
       61 cgggccggac ggctcggtcg agatccggcc gatcatgctc ttcgacgagg agggccaccc
      121 gtcgtcgtag ggaccgcgac cgccgccgtc atcgacagcc tgttccgcaa cgagcagggt
      181 cgtgcggtcg cgaccctgat ccgggtcctg ggagatttcg acctcgccga ggacgcggtg
      241 caggaggcgt tcgcgcaggc gatgcgcgtc tggcccgagc gcgggatccc cgacaacccc
      301 ggcgcgtgga tcacgacgac agcgcgcaac cgcgcgatcg accggctccg gcgcgagcgg
      361 gcgctgcgcg agaagacggc gcagctcggc gtcgtcgagg cgctcgagtc gctcggagga
      421 gacgagacgg acgtgagtgg gatccccgat gaccggctgc ggctgatctt cacgtgctgc
      481 catccggccc tgcggatgga cgcgcgggtc gcgttgacgc tgaagacgct cggcggcctc
      541 acgaccggcg agatcgccca tgccttcctc gttccggagc cgacgatggc ccagcgtgtc
      601 gtccgggcga agcgaaagat ccgtcaggcg gcgatcccct accgcgtccc gcccgccgag
      661 ctcctgcccg aacgcgttcc gggcgtgctc gccgtcctgt accttgtctt caacgagggg
      721 tacgcgccca ccaccggccc actcgttcgg acggacctgt gcgacgaggc gatccgcctg
      781 gcccgtctcg tcgtgacgct gatgcccgac gaacccgaga tccggggcct gctcgcgctg
      841 atgctcctgc agcattcgag gcgcgaggcc cggaccgatg cgctgggggc gatgatcctg
      901 ctcgaggacc aggaccgggc ccgatgggac cacgcgatga tcgacgaggg cctcgccacc
      961 ttggacggcg cgctcgccgc ccgccggcct ggtccgtacc agctccaggg cgcgatcgcc
     1021 gcgctccacg cccgagcgcc ccgacccgag gacaccgact ggccacagat cgccgccctc
     1081 tacgaccggc tcgtcgagac ctcgccctcg ccggtggtcg agctcaaccg cgcggtcgcg
     1141 atcgcgatgg ccgatggtcc cgagcgcggc ctcgcgctct tcgcgccgct ggaggtcgag
     1201 ctcggcgagt accacccgtt ccatgccgcc cgcgccgacc tgttccgtcg ggtccgccgc
     1261 aacgaggagg cggccgccgc gtaccgccgg gcgctcgagc tcgtgacgaa cccgcggcag
     1321 cgcgcctacc tcgagggcag gctcgcggag gtcacaggtc cttgaccagg taggtcatca
     1381 ccgcgccgac ggcgaccggg ccgtcgtcat ggagtagatc ggtgctggag acgaacggcc
     1441 cgtgcgcgtg gacgtacccg agccgctcgt agagcgccct cgcgggggcg ccctcctcgt
     1501 cgagagagac cgacaggccg accctggtca tgccgcggtc ccggaccgct tcctcgagcg
     1561 tctccatcag catcgtcgcg acgccgagcc ggcgcctggt ccgcgcgacg aagaggtcac
     1621 gcacctccgc gcagccttcg cggaccgccg acgtcgagaa ctcctcgtgc tcggggaaca
     1681 gcaccatgcc cctgccgccg gggaggtccc cgaaccacgc gaccgcctcg acgagctccc
     1741 cgcgtccctg ggccagcagc cgccgaaggt actcgcccga gttccacacg gggaggcgcc
     1801 ggttgagcgt ctcgacatcg cccgccgaga gtggcaggac gcggaaggcc gaggtcgacc
     1861 gcgtcgatcc gggatcgccc gccggcgccg agatcgccct cagccctcgt tcgaggtcgg
     1921 aagggcgcgg cccatggagt cgtgcgcgtc gaggacgccg ctcatgtgcc acttccgggg
     1981 ccggatcagc tcctcggcct cctccggtcc ccacgacccc cgctcgtaga agcagaccgg
     2041 cgtcgggtgc tcgagcgccg gctggaggat ctcccacgcc cgctcgacgc cgtcttggcg
     2101 gatgaacagc gaatggtcgc cgcgcatggc ctcgaggagc agcagctcgt aggcgtcgga
     2161 gatctccgtg tgtgcgaacg cctcgtcgta gtcgaagtcc atcgccgccc tgcccagatg
     2221 catacccggt cccggctgct tgacgttgaa gccgatcgtg atgccctcgt tcggctgcac
     2281 gcgcaccgtg aggtggtcgc gcatcggcag gtccatgtcg gaccctcgga acacgttgaa
     2341 cggcaccttc ctgaacttca gcgtgatctc gctcatcttc tcggcaagcc ccttgccggt
     2401 ccgcaggaag aacgggacgc ccgcccatcg ccagttgtcg atgtcgagct ccatcgccga
     2461 gaaggtctcc gtcgtcgagt cgggggcgac gccgtgttcg tcgcggtacc cctcgtactg
     2521 gccccggatc acgcgatccg ggctcgagat atcggtcgat ctcagggcct tcacggtctc
     2581 gtcccggagc cggtcgggtg cgaacgacac gggcggctcc atcgcgacga acgtcatcac
     2641 ctggaacagg tgcgtcgaga tcatgtcccg gatgttgccg aactcctcgt agaaggccac
     2701 gcgcccctcg atcccgatct tctcggcgac cgtgatctgc acgtggtcga tgtagcgacg
     2761 gttccagatc ggctcgaaca tcccgttcgc gaaccggaag gcaaggatgt tctgcacggt
     2821 ctccttgccc aggtagtggt cgatgcggaa gacctgcatc tcgtcgaaca cggagtggat
     2881 gacctcgttc aggtcgcgcg cgctctgcag atctcgcccg aacggcttct ccagcacgat
     2941 cttcgcgtcc cgctccagac cggcctcgcc gatccgccgg acgatgtcgg cgtacaccgt
     3001 cggtggcacc gccgcgtcga agatccggcg cccctgcgtg ccgcactcgg cgtcgatctt
     3061 ctccaggtac gtcgcgaagt cgttcatcgc gccgggctcc gagaactcgc cctggtggta
     3121 cgacaggcgc cgcgcgaact cctcccaggc ctcgccctcg ggcgggtgct tggagaactt
     3181 gctgacgtgg tcgaacgcgg actgccggaa ctcctccgag gttatcgccg agcgcgcgta
     3241 cccgaccagc gcgaacccct tgggcagccg ccgctgcagc cacaagtgcc agaaggcggg
     3301 caacagcttt ctcttggtca gatcgccgga cgcgccgaag atcacggccg cctgtgggag
     3361 gagctccctg gccatcacca acccccttcg gacggcggcc ggacctcgat ccactcgtcg
     3421 gtcacgcgaa cggcgtatac cggcaccgga tcgaccgccg ggcccccgac gggctcgccc
     3481 gtggcgaggt cgaactcgct cccgtggcac gggcacacca cggtgtcctc ctggtcgccg
     3541 agcgtgccct ctgccagcga gcacccctcg tgggtgcatt cgtcgccgaa cgcgtacagc
     3601 gcgttctcga cgtgcgcgat cgctacacgc aggccgggga ggtcgaagga gcggagctct
     3661 ccctcgggaa cctcggcgcg cgagccgacc cgccgatacg actcgtccac gcgtccatct
     3721 tcgcccacgg agcggtctcc gatgtggacg tcgctccgtt ccgcggtccc gtcacgcacc
     3781 gagggtcgcg ttcgggtcgg aagcggtctc gcgcgtgggc tcgacggggg gaccctgttc
     3841 gcggacggcc acctcgtcga ccccgccccc cgaactcacg gcgaggaccc tggtcaccgc
     3901 ggaccacacg cgctgcgcca gagtgacggc gcccagcacc gtcaggatcg cgagcgccgg
     3961 cagcagggca cccgggatgg ggaggccgat catcatcgcg atatagcgtt cgagccgctg
     4021 gaagtagccc tcggtgagct cgacgccggc ggcctccgcc tcggcgcgga cgtggctcac
     4081 gcagagcgcg atcaccagcg tgagcagcgc gagggccgcc tcgagccgga gaccttgccc
     4141 ggcgagcgac cagaacaggc atccgaacag gatcacgtcc gccacccggt ccaggaccga
     4201 gtccaggaac gcgccccagc ggctgtcctc gccgcgaagc ctggccacgg cgccgtccag
     4261 gatgtcgaac agtcccgccg ggatcagcag cagccccgcc agcaggaact ggccccggag
     4321 cagcgacgcc ccgacccaga cgttggtgac cagaccgagc accgtcagat gccacggccg
     4381 gaacccggcc cggtagacgc cgaggaggat gacgcgatac ggccacgtga atgcccagcg
     4441 catcggcttg gcggcgacgc cctcggccac gcgaggcgcg ggcatgtcgc ggatgcgcgt
     4501 ggtctcgctc ggcggttccg gctgggttcc gctcatctga tcccgctccc gaggctatcg
     4561 ccgcggcgcg gcggccgcat ccgctcgggg acgacctcag gttcgaggct cgaaggcgac
     4621 gacacggtaa tggaaggtcc ctccgggcgc ctcgtacgcg atctggtcct cgaggttcgc
     4681 ccccaggagt gccttgccga gcggcgacgt ggtcgtgatc gtccgcacac ccgactcacg
     4741 ctcctcggcg gactcggcga gcaggtagat ctcgtcctcg ggttcgtcct cctcgagcgg
     4801 acggagcgtc acgagcatcc cggagacgac cacctcggtc tcggcgggtc cctcgacgat
     4861 ctcgggatcg cgaagcatgc gttcgaggtt gcggatccgg gactccatca gcgcctgctc
     4921 gttcttcgcg gcatcgtact cggcgttctc ccggatgtcg ccgtgctcgc gagcggcctt
     4981 caggcgttcg gcgatcgcga cgcggcccga gatcttcagg tcgtcgagct cgccgcgcag
     5041 gcgcaggtag gcgtcgagcg tcagggtcga cgtcggcagc tgcccgccct tgcggggagg
     5101 ccggttcgcg agctcttcgg gcagttccat gagatgggcg agcctaccaa ctcgggccga
     5161 gcggccccct aagcagctga agggaacggc cgctacgact ccgcgaagaa gacgttcttg
     5221 acctcggtgt acaggttgac ggcgtgcatc cccatctccc ggccgatccc gctctgcttg
     5281 tagccgccga acggggcctc ggtgtggacg ctgtgcgccg agttcaccga gatcacgccg
     5341 gtccggatcg cc
//
LOCUS       DATBRK010000024        14930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2313940, whole genome shotgun sequence.
ACCESSION   DATBRK010000024 DATBRK010000000
VERSION     DATBRK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14930
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313940"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VIH70_00740"
     CDS             complement(<1..324)
                     /locus_tag="VIH70_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY8114918.1"
                     /translation="MTERLQQMDRDRIVERIWRKDHTVWKQDPTEITDRLGWLTVSDL
                     MHERIGELEAFAEQASADGFETAVLLGMGGSSLAPEVFVRTFGSADGSLELIVLDTTH
                     PETIRR"
     gene            complement(422..1084)
                     /gene="rpe"
                     /locus_tag="VIH70_00745"
     CDS             complement(422..1084)
                     /gene="rpe"
                     /locus_tag="VIH70_00745"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY8114919.1"
                     /translation="MPNDVLLAPSILAADYAVLAEEVERVRVESDWLHVDLMDGHFVP
                     NLSIGPPVVASIRRHTDLFLDCHLMVTDPGFWLERLAEAGADLCSVHVELGDPTPLLT
                     RIRELEMQAGVVVDGPTAFEAVEPFLDERIDLLLVMTITAGFGGQPFVPEHLSKVERA
                     AEIRRSRDLPFRIQVDGGIGPETAARAVAAGADVLVAGSAIFHAPDPRAAARAIRAAA
                     AR"
     gene            1216..2433
                     /locus_tag="VIH70_00750"
     CDS             1216..2433
                     /locus_tag="VIH70_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8114920.1"
                     /translation="MDDVIRVYGASWCPDCRRAKRFLGDQRISFEWHDIEVDPDGVRT
                     VQERNDGNNVIPTIIFPDGSHLSEPSNEELAEKIGLERMAMMHVYDLIIVGGGPAGLT
                     TSIYAARENLQTLVIDSKGLGGQAGVTERLDNYPGFPEGIGGAELADRIVLQAQRYGV
                     EMLQAVSVRSISSDGEEVDVETATGDHYHSRAALVATGSTYRMTGAPGEDDLIGAGIH
                     FCATCDGPFYKGANQLVVLGGGNSGLEEGLFLTQFVDTVTIVQRSDRLTASKLLRDKV
                     MMTPTMEVLLNREVVEFRPKDDGSGKLGAVALRNRETGDTEELHPAGAFVFIGLDPNT
                     GFVKGSVDLDDRGLIVTDMGLQTSMPGVFAAGDVRSGSTKQLASAVGEGAAAAIGIRM
                     YLEALDAKPMHMM"
     gene            2468..2689
                     /locus_tag="VIH70_00755"
     CDS             2468..2689
                     /locus_tag="VIH70_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114921.1"
                     /translation="MEPQEIFELIIKADEKLKYAHEDNAGLRRQQARDLLERAREAAT
                     EIGNDALVQQADTRLADLGGPEGEAGPAT"
     gene            complement(2763..3797)
                     /locus_tag="VIH70_00760"
     CDS             complement(2763..3797)
                     /locus_tag="VIH70_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY8114922.1"
                     /translation="MTTTRAHLVTRLSIAVLLVASATIGAATLSSAAPSREEVEQARA
                     RLDSLNRELDLMVEQYNQARLKLQEVQARLADVRAQAHRAQATADRAISSLNANAARA
                     YRGVGSQIAQLFDSASLADFSDRLEFIGSMAQADSDLAVEAQAARQEARWTAAELRAT
                     AEERQGIVDALAAKQDQIESRVNEARDLYEELDREYHEALAAQASAQGVASASTGGGV
                     SPIPPPPAPNANVGAVLQAAYSMIGTPYQYGGSSPETGFDCSGFTMWAWSHAGVYLPH
                     SSAAQYASLPHVAQSDLQAGDLVFFYSPISHVGIYVGGGSMIHSPQTGSVVSVVPIYW
                     DSFAGAARPG"
     gene            complement(3914..3990)
                     /locus_tag="VIH70_00765"
     tRNA            complement(3914..3990)
                     /locus_tag="VIH70_00765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3954..3956),aa:Pro,seq:cgg)
     gene            4084..5118
                     /locus_tag="VIH70_00770"
     CDS             4084..5118
                     /locus_tag="VIH70_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8114923.1"
                     /translation="MRIAEICPPWIAVPPKGYGGIEWVVALLADGLAEAGHDVTLFAT
                     GDSQTKARLEYVFEQAPGSSAINDIVLDTTHTLYSLKDAAERFDVLHVHSPFSALAAA
                     VETGVPTVHTLHGSFVPEMTRLYSFVAQRAWFVAISEAQKRFNEDLRYGGVVYNGIDM
                     TAYPLQEDKEDFVLFLGRAAPEKGWRRAVEAAVAAGERLVSAVKIAHPTEQEEWTNNV
                     QPILPPESEVVGEITLAEKVSLLRRAKAVLFPIDWPEPFGLVMTEAMACGTPVIATPR
                     GSVPEVVEDGVTGWIVDVEDYPRQAAERLARLSEIDPQACRDRVQRLFSKEAMVAGYE
                     AAFELAASGP"
     gene            complement(5140..6051)
                     /locus_tag="VIH70_00775"
     CDS             complement(5140..6051)
                     /locus_tag="VIH70_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8114924.1"
                     /translation="MLARILFALAGIAVVCISYGVAIERRAYRLVRRGLAILPADGPE
                     TLTVLHLSDLHFVTDDPRKHRFLASIPRADVTVVTGDFLAEPEAVETAVAAVRGTRGR
                     LASWFVLGSNDYYVSRATNPLRYFVRPGRRKRRRPRRGRSEDLRALLCADGWSDLTNR
                     RDELDLGGLAIELLGLDDAHIRRQDLRLAPRRSPSRFGIAVMHSPDSAPEISSCGYAF
                     VFAGHTHGGQVRLPFVGALVTNSHLPRRLVSGLIRMGGSFVHVSPGLGTSRYAPFRFL
                     CPPEATLLELRRARDGGSPARTGAHSQ"
     gene            6067..6522
                     /locus_tag="VIH70_00780"
     CDS             6067..6522
                     /locus_tag="VIH70_00780"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY8114925.1"
                     /translation="MGDPTLKAQVREQMTAALRVGDKLRLGALRMLAASITNREKEVM
                     HELSDEEVREVAAKEVKRRTESIEAFESAERNELAEKERAEREVLEPFAPERLADAEV
                     DALIDGAIAATGATSMQDLGKVMGAVMASAKGKVDGSVVQRKVRERLAG"
     gene            complement(6526..9105)
                     /locus_tag="VIH70_00785"
     CDS             complement(6526..9105)
                     /locus_tag="VIH70_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8114926.1"
                     /translation="MRAPPRHRPRSHEPLQTREETVRVPDLRPRLVIAPLVLFVLIAV
                     MAGFAAVLALALLPIFGAAGGGVNAFRERLDAAGVGRAGIPHLPQSSTIYAADGKTVL
                     AEIYLDENRRYVRIGKIAPVAQQAVVAIEDDSFYEHGALNFPSLMRAAITNLIAGDIE
                     QGGSTLTQQLVKNVLIDSPQQTFARKFQEAALAIRVERKYSKDHILELYMNEAYYGNG
                     AYGIQTAAETYFDTTARDLSLGQAALLAGVIRAPGTYDPVAHPDAARARRNLVLERMS
                     ELGVIDPADAAKAQAKPLGIAKNAGEFKEKVEPFFVYYIRNLILDNADGEFDAFGQRR
                     IERVHTLYQGGLNIYTSLDPSWQEYAQEAVDASPAIHPGRNSPDVSLVSVRATDGAIK
                     AMLSGKNYDRDQYDLVWRGTRQVGSAFKPFTLTAAFEEGIPPGKVYSSESPLCNLEGW
                     RSASGCVSNAEGGGDSGYLDLWSATQDSVNVVFAQLALDVGPEHIVDVAHRMGITVPL
                     DAVPSITLGVEEVPTMDMAAAFGTLANDGKRCNAWAVRRVEFANVPKDAPKDERVLYQ
                     HEPECEQVIKPEIAHLVTAMLQRVVCCGTGTAANIGRPVAGKTGTAQDYTNVYFAGFT
                     PQISTAVWVGFANGQIPMDTYYGGSVFGGTVAAPIWHDFMVKAMAGLPVEGFEAPPPP
                     EYGPVPGVIGLAIEEAETKLVKATLTPIREQVRSFEPAGRVVSQAPGGGTRLRLGSAV
                     RLGVSDGKGEAVVIPRLTGLTQAEAERRLERLGLVAAVERVPVDDKHLDGIVVDQAPI
                     GDGSKMVNLGATVTIDVGAFDQGNGSGGGTGEGGNGNGGGHGGVDEGSPAMPSEALFP
                     RAI"
     gene            9156..10475
                     /locus_tag="VIH70_00790"
     CDS             9156..10475
                     /locus_tag="VIH70_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY8114927.1"
                     /translation="MRRAAPFLLFSALMCTVAGSAGAQDTPSVLVAKVDGSIDRTLAS
                     YLVDSIEEAEREGSTLVLQLDSAGTLDQEAVALAERIHDASVPVIVWAGPAPARAAGA
                     GLLFLYAASLGAVAPGAGVGPLEPLDLAGDEPEIPVGEVEALATGWVQERGRETPLSF
                     PDRPVPARAALDGHVASVAAVSITDLLDRVNGRTVGTSDGEVTLETRIATSEAEQRVT
                     VRFTDLGPFDRVLHAAASPTWIYVLLVLGLAALAFEATQPGFGFAGFSGLGMLALAVY
                     GLTVVPFSALGLGLLLAGIGLLTLDVRLRRLGPLSIVGVLAFVAGSVLVFGGVADEID
                     VSPWLIGSCSIAALLYWGFGLTVAVQSRDRLTSTQRGLVGLVGEARGELMPDGPVFVK
                     GALWRGRSSDGPIPAGSRIRVRGVDGLILRVEQEPPPVQGPDLPNEA"
     gene            10651..10920
                     /locus_tag="VIH70_00795"
     CDS             10651..10920
                     /locus_tag="VIH70_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY8114928.1"
                     /translation="MRAGWTWGWQFDGACRGEDSSLFFAPSYFEKREEKAAREVKAKA
                     ICTGCPVREPCLDYALRIREPHGIWGGLNEYERRQRLRQQALQAG"
     gene            10931..11386
                     /locus_tag="VIH70_00800"
     CDS             10931..11386
                     /locus_tag="VIH70_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8114929.1"
                     /translation="MIEERLAELGIELPPAPQPVASYIPVKVVGDLAWVAGQVPMQDG
                     AVTVGGSVGADVTTEEASAGARRCAIQALAALRAALGSLDRVKGIVKVDVFVASAPGF
                     SEQPMVANGASDLLVDVFGDEGRHARAAVGVPELPLGAAVEVALLVQLG"
     gene            complement(11374..13296)
                     /gene="selB"
                     /locus_tag="VIH70_00805"
     CDS             complement(11374..13296)
                     /gene="selB"
                     /locus_tag="VIH70_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEY8114930.1"
                     /translation="MTERAGASAPGAPEHPGPSVVATAGHVDHGKSSLVTRLTGMDPD
                     RLGEEKRRGLTIELGYAWCTLPSGRELGFVDVPGHERFVRTMLAGVGPVPLVLFVVAA
                     DEGWKPQSEEHLEIVDVLGSDGAVIALTKRDLVEAAGLDRVRAEVSERVRGTVLQGAP
                     IVACSSTTGEGFDELVAALDAMIAGAPEPDRGGRPRLHVDRVFSIRGSGTVVTGTLTG
                     GPLATGADVELLPSGTRARIRGLQTHHRGVETASPVSRVAVNLTAERTRIARGEVLAP
                     AGLWTATSVVEARIEPVRSLDHPITSRGAFKLHAGAAERDARIRLYDVKQVPPEGAFV
                     RITLSEPLVLDVRDRFVLRESGRRRTIAGGIVLDTDPPRRAGPTPAHRLGARARASRA
                     ELPALLVAERRAVRGDEVRSILGTVPEVLDGAVRLGGWWVHHDLPGEIGAAITARLAV
                     FHAAEPSAAGEGIATTRRSIADALAAAGVPHDLELADALVDRLETEGTLVREGALVRL
                     ASHRAISGDEVARVVEVVRSAEPTPPSIAELRAAGVAPAVLDAAVRSGALVRISPELV
                     LTRGLVERAGTIVREAGAGGITVSALRGRLGTSRKYAVPLAEHLDRTGVTRRSGDLRF
                     ARGATSASSGSPAQPS"
     gene            complement(13289..14713)
                     /gene="selA"
                     /locus_tag="VIH70_00810"
     CDS             complement(13289..14713)
                     /gene="selA"
                     /locus_tag="VIH70_00810"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY8114931.1"
                     /translation="MARAERERTGNAITTAGREPRRRIPSVDALLRSEPGRRASGTFG
                     RPLVKHALSVTLEQVREAAEGGTEPPPHDEILARALGLTSATAAGLVRVINATGVVLH
                     TGLGRAPLPARAAAAAARAGQGYVDLEVDRGTGARERRSTRAERLLTSMTGAEDALVV
                     NNGAAALLLALASLARGKGVLVSRGELIEIGGEFRIPDIMAASGARLVEVGTTNRTRA
                     SDYRSALSDKTALILKVHPSNYRVVGFASTPSAKDLAGIARRANVPFLYDLGSGLLER
                     VPGAAAEEPSATGALADGADLIAFSGDKLLGGPQAGIVLGRSELIERLRRHPIARAVR
                     IDKMQVAALESVLAQYAHGLGSGLPVWTMLREPPTRVRRRADELAMALDGELEGAHVL
                     ACESAVGGGALPGTGIPSFGVEIRVPQPNAMAARLRTGSPSVFCRVTERGVLLDLRTV
                     SPEEMPDLVRAVRYALEGDDLPDD"
BASE COUNT         2123 a         5264 c         5418 g         2125 t
ORIGIN      
        1 tcgccggatc gtctcggggt gcgtcgtgtc gaggacgatg agctcgagcg agccgtccgc
       61 ggatccgaac gtgcgcacga agacctccgg cgcgaggctc gaacccccca tcccgagcag
      121 gaccgcggtc tcgaacccgt ccgccgacgc ctgttccgcg aacgcctcga gctcgccgat
      181 ccgctcgtgc atcaggtcgg agaccgtgag ccagccgagc cggtcggtga tctcggtcgg
      241 gtcctgcttc cagacggtgt ggtccttgcg ccagatccgc tcgacgatcc ggtcgcgatc
      301 catctgctgc aggcgttcgg tcacgtcgtc tcctttcgag acccgtcgca gaaggggggg
      361 cgcccacggt agtcgctctc ggaccgcggg gtcgagggcg gttcgcccgc acgaccggat
      421 gctaccgagc ggcggccgcg cggatcgccc gtgccgccgc gcgcggatcg ggtgcgtgga
      481 agatcgcgct gccggcgacg aggacgtcgg ccccggccgc gacggccctg gccgcggtct
      541 cgggcccgat gccgccgtcg acctggatgc ggaacgggag gtcccgcgat cggcggatct
      601 cggcggcgcg ttccaccttc gacaggtgct cggggacgaa cggctggccg ccgaacccgg
      661 ccgtgatcgt catcaccagc agcaggtcga tccgctcgtc gaggaacggc tcgaccgcct
      721 cgaacgccgt cggcccgtcg accacgaccc ccgcctgcat ctcgagctcg cggatcctcg
      781 tcagcagagg cgtcgggtct ccgagctcca cgtgcacgct gcacagatcg gcccccgcct
      841 ccgcgagccg ctcgagccag aaccccgggt cggtgaccat caggtggcag tccaggaaca
      901 ggtcggtgtg ccggcggatc gacgcgacca ccggcggccc gatcgacagg ttgggcacga
      961 aatgcccgtc catcaggtcg acgtggagcc agtccgactc cacgcggacc cgctcgacct
     1021 cctcggcgag cacggcgtag tcggccgcga ggatcgaggg ggcgagcagg acgtcgttcg
     1081 gcacgggcgt agtctgcccc accctcggtc gccggcgctc ggggcggatc ctcgacctcc
     1141 ggggaccggg tgtgtaacgc tcgccgccgg atccggtact ggctacggat cggctggttc
     1201 ggagagggga gtgtgatgga cgacgtcatc agggtctacg gggcaagctg gtgcccggac
     1261 tgccgaaggg ccaagcgctt cctcggggac cagcggatct cgttcgagtg gcacgacatc
     1321 gaggtcgacc cggacggggt ccgaaccgtg caggagcgca acgacggcaa caacgtcatc
     1381 ccgacgatca tcttcccgga cggctcccac ctgtccgaac cctccaacga ggagctggcc
     1441 gagaagatcg gtctcgagcg catggcgatg atgcacgtct acgacctgat catcgtgggc
     1501 ggcgggccgg ccgggctcac gacttcgatc tacgcggccc gcgagaacct gcagacgttg
     1561 gttatcgaca gcaagggcct cggcggccag gcgggcgtga ccgagcgcct cgacaactac
     1621 cccgggttcc ccgagggcat cggcggcgcg gagctcgccg accggatcgt cctgcaggcg
     1681 cagcgctacg gcgtggagat gctgcaggcc gtgtccgtgc gatcgatctc gagcgacggc
     1741 gaggaggtcg acgtcgagac cgcgaccggc gaccattacc actcacgcgc ggcactcgtc
     1801 gcgaccggct cgacgtaccg gatgaccggg gcgccgggtg aggacgacct gatcggcgcc
     1861 ggcatccact tctgcgcgac ctgcgacggc cccttctaca agggcgcgaa ccagctcgtc
     1921 gtcctcggtg gcggcaacag cggtctggag gaggggttgt tcctcacgca gttcgtggac
     1981 acggtgacga tcgtgcagcg gagcgaccga ctgaccgcca gcaagctgct ccgggacaag
     2041 gtgatgatga cgccgacgat ggaggtcttg ctcaaccgag aggtcgtcga gttccggccc
     2101 aaggacgacg ggagcggcaa gctgggcgcg gtcgccctca ggaaccgcga gacgggggac
     2161 accgaggagc tgcaccccgc cggcgcgttc gtgttcatcg ggctcgaccc gaacacgggg
     2221 ttcgtcaagg gctcggttga tctggacgac cgaggcctga tcgtcacgga catgggtctg
     2281 cagacgagca tgcccggggt gttcgccgcg ggcgacgtgc gttcgggctc gaccaagcag
     2341 cttgcgtcgg cggtcggcga gggcgccgcg gccgcgatcg gcatccggat gtacctcgag
     2401 gcgctcgacg ccaagccgat gcacatgatg tgaggcgccg agcaggagcg accgaaggag
     2461 cgcgtcgatg gagccccagg agatcttcga gctcatcatc aaggcggacg agaagctgaa
     2521 gtacgcgcac gaggacaacg ccggacttcg ccggcagcaa gcacgggacc tgctcgagcg
     2581 cgcccgcgag gccgcgaccg agatcgggaa cgacgccctc gtgcagcagg cggacacgcg
     2641 gctcgccgat ctcggcggac cggagggaga ggcggggccg gcgacctaag cggaggcccg
     2701 ccagtggcgg aacacgccga gaagggcttc gcgcgcgtcg ggtgcgaccc ggtcctcgcc
     2761 gatcagcccg ggcgggcggc gccggcgaag ctgtcccagt agatgggcac caccgagacg
     2821 acgcttccgg tctgcggcga atggatcatg ctgccgcctc ccacgtagat ccccacgtgg
     2881 gagatggggc tgtagaagaa caccaggtcg cccgcctgca ggtccgattg ggcgacgtgg
     2941 gggagcgacg cgtactgcgc ggccgacgaa tgcgggaggt acacgcccgc gtgcgaccac
     3001 gcccacatcg tgaagcccga gcagtcgaag ccggtctcgg ggctcgaccc gccgtactgg
     3061 tacggtgttc cgatcatcga gtaggcggcc tgcaggacgg ccccgacgtt ggcgttcggg
     3121 gcgggaggcg gcgggatcgg agagacgcca cctccggtgc tcgccgaggc caccccctgg
     3181 gcggacgcct gcgccgccag cgcctcgtgg tactcgcggt cgagctcctc gtagaggtcg
     3241 cgcgcctcgt tcacccgcga ctcgatctgg tcctgcttcg ccgcgagggc gtcgacgatc
     3301 ccctggcgct cctcggccgt ggccctgagc tcggccgcgg tccagcgggc ctcctgccgg
     3361 gcggcctggg cctccaccgc gaggtcggag tccgcctgcg ccatgctccc gatgaactcg
     3421 agccggtcgg agaagtcggc cagtgatgcc gagtcgaaca gctgggcgat ctgcgagccg
     3481 acgccccggt aggctctggc ggcgttcgcg ttcaggctgc tgatggctcg gtcggcggtc
     3541 gcctgggccc ggtgtgcctg ggcccgaacg tcggccagcc gtgcctggac ctcctggagc
     3601 ttcagccgcg cctggttgta ctgctcgacc atcaggtcga gttcccggtt gagggagtca
     3661 aggcgggccc gggcctgctc gacctcctcg cggctgggtg ccgcggagga gagggtggcc
     3721 gcaccgatgg ttgccgaggc gacgagaagc acggcgatgg acagccgtgt gacgaggtgc
     3781 gcgcgagttg tggtcatcgt cccccaggcg acaaaacggg ctgtcctacg acagcccgcg
     3841 ctcgaggcta cccgacaacc gaacctcggg caacagcggg tccggccgat attcgggccg
     3901 gggatcggag gtctggtcgg gacggcggga cttgaacccg cgacctcagc gtcccgaacg
     3961 ctgcgcgcta ccaaactgcg ccacgtcccg tcgtgcccga gtctagcggc tgggccggtc
     4021 cgcggccggc cggtagcatc gaggggtcgg cacgcccgcc gatcgcgtcg ggaggaggcc
     4081 cggttgagga tcgccgagat ctgtccaccg tggatcgccg tgcccccgaa gggctacggc
     4141 gggatcgaat gggtcgtcgc gctgctggcc gacggcctgg ccgaggcggg gcacgacgtc
     4201 acgctgttcg cgaccggcga ctcgcagacg aaggctcgtc tcgagtacgt cttcgagcag
     4261 gccccgggct cgagcgcgat caacgacatc gtcctggata cgacccacac gctctacagc
     4321 ctgaaggacg cggccgagcg cttcgatgtc ctgcacgtcc actcgccgtt ctcggcgctc
     4381 gccgcggcgg tcgagaccgg ggtgccgacc gtccacaccc tgcacggctc gttcgtgccg
     4441 gagatgaccc ggctgtactc gttcgtcgcg caacgcgcat ggttcgtcgc catcagcgag
     4501 gcgcagaagc gattcaacga ggatctgcga tacggcggcg tcgtctacaa cggcatcgac
     4561 atgaccgcct atcccctgca ggaggacaag gaggacttcg tcctgttcct cggtcgtgcg
     4621 gcccccgaga agggctggcg gcgcgcggtc gaggcggccg tcgccgccgg cgagcgcctc
     4681 gtgtccgccg tgaagatcgc ccaccccacc gagcaggagg agtggacgaa caacgtccaa
     4741 ccgatcctgc cccccgagtc cgaggtggtg ggggagatca cgctggccga gaaggtgagc
     4801 ctgttgcgcc gggccaaggc tgtcctgttc ccgatcgatt ggcccgagcc cttcgggctc
     4861 gtgatgaccg aggcgatggc gtgcggaacc ccggtcatcg cgacgccgcg cgggtccgtc
     4921 cccgaggtcg tcgaggacgg cgtcaccgga tggatcgtcg acgtcgagga ctacccgagg
     4981 caagccgcgg agcggctcgc acggctctcc gagatcgatc cgcaagcgtg ccgcgaccgc
     5041 gtgcagcgcc tgttctcaaa ggaggccatg gtcgcggggt acgaggccgc gttcgagctc
     5101 gccgcctcag gcccctgaag cctctctgca cccctgcgcc tactgcgagt gagcgccggt
     5161 tctcgccggg ctcccgccgt cgcgagcccg gcgcagctcc agcagggtcg cctcgggcgg
     5221 gcacaggaac cggaacggcg cgtatctgct cgtccccaga ccgggcgaga cgtgcacgaa
     5281 cgatcctccc atccggatga gcccggacac caggcgccgc gggaggtgcg agttcgtcac
     5341 gagggcgccc acgaacggca gccgtacctg gccgccgtgc gtgtgcccgg cgaagacgaa
     5401 tgcgtagccg caggaggaga tctcgggcgc cgagtccggt gagtgcatca cggcgatccc
     5461 gaacctcgag ggcgagcggc ggggcgcgag gcgcaggtcc tgacgccgga tgtgcgcgtc
     5521 gtcgagtcca aggagctcga tcgccagccc accgaggtcg agctcgtcgc gccgattcgt
     5581 gaggtcgctc cagccgtccg cgcacagcag cgcgcgcaga tcctccgacc ggccgcgccg
     5641 agggcgccgg cgcttccggc gaccgggtcg gacgaagtac cggagcgggt tcgtcgcccg
     5701 agagacgtag tagtcgttcg agccgagcac gaaccaggac gcgagccggc cgcgcgtccc
     5761 ccgcacggcg gcgaccgcgg tctcgacggc ctcgggttcg gcgaggaagt cgccggtcac
     5821 gaccgtgacg tccgcccgag ggatcgacgc gaggaacctg tgcttgcgcg ggtcgtcggt
     5881 cacgaagtgc aagtccgaca ggtgcaggac cgtgagggtc tccggaccgt ccgccggtag
     5941 gatggccaga ccgcgacgca cgagccggta cgctcggcgt tcgatcgcga cgccgtagga
     6001 gatgcatacc acggcgatgc cggcgagcgc gaacaggatg cgggcgagca cgggagggga
     6061 gtctacgtgg gcgatcccac gctgaaggcg caggtgcgcg agcagatgac cgcggccctg
     6121 cgggtcgggg acaagctccg ccttggcgcg ctccgcatgc tcgcggcgtc gatcacgaac
     6181 cgcgagaagg aggtcatgca cgagctctcc gacgaggagg tccgcgaggt cgcggccaag
     6241 gaggtcaagc ggcggaccga gtcgatcgag gcgttcgagt cggcggagcg gaacgagctc
     6301 gccgagaagg agcgggccga acgcgaggtg ctggagccct tcgcccccga acggctcgcc
     6361 gacgccgagg tggacgcgtt gatcgacggc gcgatcgcgg cgacgggcgc gacctcgatg
     6421 caggacctgg gcaaagtcat gggcgccgtg atggcctcgg ccaagggcaa ggtggacggc
     6481 tcggtggtgc agcggaaggt gcgcgagcgt ctcgcgggct gagcgctaga tcgcccgcgg
     6541 gaacagggcc tcggacggca tcgccggcga gccctcgtcg acacccccat gaccaccccc
     6601 gttgccgttc ccgccctccc ccgtcccgcc accgctcccg ttgccctggt cgaaggcgcc
     6661 gacgtcgatc gtgaccgtgg cgccgaggtt gaccatcttc gatccgtcgc cgatcggcgc
     6721 ctgatccacg acgatcccgt ccagatgctt gtcgtcgacc gggacccgtt cgaccgcggc
     6781 gaccagcccg agcctctcga gccggcgctc ggcctcggcc tgggtgaggc cggtcagacg
     6841 tgggatcacg acggcctcgc ccttgccgtc gctcacgccg agcctcaccg cgctccccaa
     6901 ccggagccgg gttccgccgc ccggggcctg cgagacgacc ctcccggccg gctcgaacga
     6961 ccggacctgc tcacggatcg gcgtgagggt cgccttcacc agcttcgtct ccgcctcctc
     7021 gatcgcgagg cctatgactc cgggcaccgg cccgtactcg ggcggcggcg gcgcctcgaa
     7081 gccctcgacc ggcaggcccg ccatcgcctt gaccatgaag tcgtgccaga tcggcgcggc
     7141 cacggtgcca ccgaacaccg accccccgta gtacgtgtcc atcgggatct gaccgttcgc
     7201 gaaccccacc cagaccgcgg tcgagatctg gggcgtgaag ccggcgaagt acacgttcgt
     7261 gtagtcctgc gcggtgccgg tcttcccggc cacgggacgg ccgatgttcg ccgccgtgcc
     7321 ggtcccgcag cacaccaccc gctgcagcat cgcggtcacc aggtgcgcga tctcgggctt
     7381 gatcacctgc tcgcactcgg gctcgtgctg gtacaagacg cgttcgtcct tcggggcgtc
     7441 cttcggaacg ttcgcgaact ccacccgccg gacggcccac gcgttgcatc gcttgccgtc
     7501 gttcgcgagc gtcccgaacg cggccgccat gtccatcgtc gggacctcct cgacgccgag
     7561 cgtgatcgag ggaaccgcgt ccagtgggac ggtgatcccc attcggtgcg ccacgtcgac
     7621 gatgtgctcg ggtccgacgt cgagcgcgag ctgcgcgaag acgacgttca ccgagtcctg
     7681 tgtcgcgctc cacaggtcca ggtacccgct gtcccccccg ccttcagcgt tgctcacgca
     7741 gccgctggcg ctgcgccatc cctcgaggtt gcacagcggc gactcggacg agtagacctt
     7801 gcccggcggg attccctcct cgaacgccgc cgtcagcgtg aacggcttga aggcggaccc
     7861 cacctgccgg gtcccgcgcc agaccaggtc gtactgatcc cggtcgtagt tcttgcccga
     7921 gagcatggcc ttgatcgcgc cgtcggtggc ccggaccgag accagcgaga cgtccgggct
     7981 gttcctccca gggtggatgg ccgggctcgc atcgaccgcc tcctgcgcgt actcctgcca
     8041 cgaggggtcg agggacgtgt agatgttcag ccctccctga tagagcgtgt gcacgcgctc
     8101 gatccgcctc tgtccgaacg cgtcgaactc cccgtcggcg ttgtccagga tcaggttccg
     8161 gatgtagtag acgaagaacg gctcgacctt ctccttgaac tcgccggcgt tcttcgcgat
     8221 cccgagcggc ttcgcctggg ccttcgcggc gtcggcggga tcgatcaccc cgagctccga
     8281 catccgctcg agcaccaggt tccggcgcgc cctcgcggcg tcggggtgcg cgaccgggtc
     8341 gtacgtgccg ggcgcccgga tcacgcctgc gagcagggcc gcctgcccga gcgagagatc
     8401 cctcgcggtc gtatcgaagt acgtctcggc cgccgtctgg atcccgtagg cgccgttccc
     8461 gtagtacgcc tcgttcatgt agagctcgag gatgtggtcc ttggagtact tgcgttcgac
     8521 gcggatggcg agcgccgcct cctggaactt ccgcgcgaag gtctgctgcg gcgagtcgat
     8581 cagcacgttc ttcacgagct gctgcgtgag cgtcgagccg ccctgctcga tgtcgccggc
     8641 gatcaggttg gtgatggcgg cgcgcatcag ggaagggaag ttgagcgcgc cgtgctcgta
     8701 gaaggagtcg tcctcgatcg cgaccaccgc ctgctgggcg accggggcga tcttgccgat
     8761 cctgacgtac cggcggttct cgtcgaggta gatctcggcg aggacggtct tgccgtccgc
     8821 cgcgtagatc gtggagctct gaggcaggtg ggggatcccg gccctgccga cgccggccgc
     8881 atccaggcgt tcgcggaacg cgttcacgcc cccgcccgcg gccccgaaga tcggaagcag
     8941 ggcgagggcg agcaccgcgg cgaacccggc catcacggcg atcaggacga acaggacgag
     9001 cggcgcgatc accaggcgtg gacggagatc gggaacccgc accgtctcct cgcgggtctg
     9061 gagcggctcg tgagatctcg gccgatgccg gggaggtgcc cgcatggggg cccaaaggat
     9121 agcccgttgc ggcccgggta cgatgagcca cgcccatgcg ccgcgccgca ccgttcctcc
     9181 tgttcagcgc cctgatgtgc acggttgccg gcagtgccgg tgcccaggac acgccctcgg
     9241 tcctggtggc caaggtggac ggctccatcg atcggacgct tgcctcgtac ctcgtggact
     9301 cgatcgagga ggccgagcgc gaggggtcga cgctcgtgct ccagctcgac agcgcgggga
     9361 cgctggacca ggaggccgtc gcgctggccg agcggatcca cgacgcgagc gtcccggtga
     9421 tcgtgtgggc gggcccggcg ccagccaggg ccgcgggggc cggcctcctg ttcctgtacg
     9481 cggcgagcct cggcgccgtc gcccccgggg cgggcgtcgg cccgctcgaa ccgctcgacc
     9541 tggccgggga cgaacccgag atccccgtgg gcgaggtgga ggcgctcgcg acgggctggg
     9601 tccaggagcg agggcgcgag accccgctgt ccttccccga ccggccggtg ccggcccggg
     9661 ccgcgctcga tggccacgtc gccagcgtcg ccgcggtctc gatcaccgac ctgctcgatc
     9721 gggtgaacgg ccgcaccgtc ggaacctccg acggtgaggt gacgctcgag accaggatcg
     9781 cgacgtcgga ggcggagcag cgggtcacgg tccggttcac cgacctcggg ccgttcgatc
     9841 gcgtgctcca cgccgccgcg tctccgacct ggatctacgt cctgctcgtg ctggggctcg
     9901 cggcgctggc cttcgaggcc acccagccgg ggttcgggtt cgcggggttc agcggactcg
     9961 ggatgctggc actcgccgtc tacggactca cggtggtgcc gttctcggcg ctcgggctcg
    10021 gtctgctcct cgcggggatc ggcctgctca cgctggacgt ccgtctgcgc aggctcgggc
    10081 cgctctcgat cgtcggggtg ctcgcgttcg tcgccgggtc ggtgctggtc ttcggcggcg
    10141 tcgccgacga gatcgacgtc tccccgtggc tgatcgggtc gtgctcgatc gccgcgctcc
    10201 tgtattgggg tttcggcctg accgtcgcgg tgcagtcccg ggaccggctc acctccaccc
    10261 agcgagggct ggtggggctg gtcggggagg cccggggcga gctcatgccc gacggtccgg
    10321 tcttcgtgaa gggggcgctg tggcggggcc ggagctccga cgggcccatc ccggccggga
    10381 gccggatccg cgtccgcggg gtggacggcc tgatcctgcg cgtcgagcag gagccgccgc
    10441 ccgtccaggg cccggatctg ccgaacgagg cctagtcccc ggtcctcgtt cgacgtcggc
    10501 ccggcccgtg gcgccgggtc gccgccccct tctgttgcga ttccctgcgt atccactcgg
    10561 ccgagcaaac cgcggaatcc cccggacgtt cagttacgaa agacctatcg aaggcatcgg
    10621 acacgggaga gcgacccggt ggttgagcgg atgcgcgccg gctggacctg gggctggcag
    10681 ttcgacggag cgtgcagggg cgaggattcg tccctgttct tcgcgcccag ctacttcgag
    10741 aagcgcgagg agaaggccgc ccgcgaggtc aaggccaagg ccatctgcac cggttgcccg
    10801 gtccgcgaac cttgtctcga ctacgccctt cggatccgcg agccgcacgg gatctggggc
    10861 gggctgaacg agtacgagcg gcgccaacgc ctccgccagc aggccctcca ggcaggctag
    10921 ggtggtcgtc gtgatcgagg aacggctcgc cgagctcggc atcgagctcc cgcccgcccc
    10981 gcagcccgtc gcgtcgtaca tccccgtgaa ggtcgtcggc gacctcgcct gggtggcggg
    11041 gcaggtcccg atgcaggacg gggccgtgac cgtcgggggt tcggtgggtg ccgacgtgac
    11101 caccgaggaa gcgagcgccg gtgctcgtcg gtgcgcgatc caagcgctcg cggcgctccg
    11161 cgcggcgctc gggagcctgg atcgcgtgaa gggcatcgtg aaggtcgacg tgttcgtcgc
    11221 ctcggcgccc gggttcagcg agcagccgat ggtcgcgaac ggtgcgagcg atctgctcgt
    11281 cgacgtcttc ggggacgagg gccggcacgc tcgcgcggcg gtcggcgtcc cggagctgcc
    11341 gctcggggcg gccgtcgagg tggcgctgct ggttcagctc ggctgagcgg gggacccgct
    11401 cgaggcgctc gttgccccgc gagcgaagcg gaggtcgccc gaacgcctag tgaccccggt
    11461 ccggtcgaga tgctcggcga gcgggaccgc gtacttccgc gacgtgccga gccggccgcg
    11521 gagcgcgctc accgtgatgc cgcccgcgcc ggcctctcgg acgatcgtcc ccgcgcgttc
    11581 gacgagcccc ctggtcagca cgagctcggg cgagatcctg accagggctc ccgaacgcac
    11641 ggccgcgtcc aggacggccg gggcgacgcc ggccgcgcgg agctcggcga tcgacggcgg
    11701 cgtgggctcg gcgctccgaa cgacctccac cacccgtgcg acctcgtcgc cggatatggc
    11761 gcggtgggac gcgagccgca ccagggcgcc ctcccggacg agcgttccct cggtctcgag
    11821 ccggtcgacc agggcgtcgg cgagctcgag gtcgtgcggt acgcctgcgg cggcgagcgc
    11881 gtccgcgatc gaccggcgcg tcgtcgcgat gccctcaccc gcagcggagg gctccgcggc
    11941 gtggaacacg gcgagccggg ccgtgatcgc cgctccgatc tcgcccggca ggtcatggtg
    12001 gacccaccac ccgcccaggc gaacggcgcc gtcgaggacc tcgggcacgg tgccgaggat
    12061 cgaccggacc tcgtcgccgc gcacggcccg gcgttcggcg accaggagcg ccggcagctc
    12121 ggcgcgcgac gcccgcgcgc gcgctcccag gcgatgtgcc ggggtcgggc cggcgcggcg
    12181 cggcgggtcg gtgtcgagga cgatccctcc ggcgatcgtt cggcgccgcc ccgactcgcg
    12241 aaggacgaac cggtcgcgga cgtcgaggac gagcggttcg gagagggtga tcctgacgaa
    12301 agccccctcg ggcggcacct gcttcacgtc gtaaaggcgg atccgcgcgt cgcgttcggc
    12361 cgcgccggcg tgcagcttga aggcgccgcg cgaggtgatc gggtggtcga gcgagcgcac
    12421 gggctcgatc cgcgcctcca ccaccgaggt ggccgtccac aggcccgccg gggccaggac
    12481 ctcgccccgc gcgatgcgcg tgcgttcggc ggtgaggttc accgcgaccc tcgagaccgg
    12541 cgacgcggtc tcgacgcccc ggtggtgcgt ctgcagaccc cggatccgcg cccgggtccc
    12601 cgacggcagg agctcgacgt ccgcgcccgt tgcgaggggt ccgcccgtga gcgtccccgt
    12661 gacgacggtg ccggaacccc tgatcgaaaa gacgcggtcg acgtggaggc gggggcggcc
    12721 gccgcggtcg ggctcggggg ccccggcgat catcgcatcc agcgcggcga cgagctcgtc
    12781 gaacccctcg cccgtcgtgg acgagcacgc gacgatcgga gcgccctgca gcactgtgcc
    12841 ccgcacgcgt tcgctcacct cggctcggac gcggtccaga ccggcggcct cgacgagatc
    12901 gcgcttggtg agcgcgatca ccgccccgtc cgagcccagg acgtcgacga tctcgaggtg
    12961 ctcctccgac tgcggcttcc acccctcgtc ggccgcgacg acgaacagca cgagggggac
    13021 gggtccgacc ccggcgagca tcgtgcggac gaaccgctcg tgccccggga cgtcgacgaa
    13081 cccgagctcg cgtcccgagg ggagcgtgca ccacgcgtat ccgagctcga tcgtcagacc
    13141 ccggcgcttc tcctcgccga gacgatcggg gtccatcccg gtcagccgcg tgaccaggct
    13201 cgacttgccg tgatcgacgt ggccggcggt cgcgacgacg gacggtccgg ggtgctccgg
    13261 tgcgccgggc gccgaggcgc ccgcgcgttc agtcatcggg caggtcgtcg ccctcgagcg
    13321 cgtagcggac cgcccggacc agatcgggca tctcctcggg cgacaccgtc cggaggtcga
    13381 gcagcacgcc gcgttcggtg acccggcaga acaccgacgg cgagcccgtg cgcagccgcg
    13441 cggccatcgc gttgggttgg ggaacccgga tctcgacgcc gaacgacggg atgccggtcc
    13501 ccggcagcgc cccgccgccc accgccgact cgcacgcgag cacgtgcgcg ccttcgagct
    13561 cgccgtcgag cgccatcgcc aactcgtcgg cgcggcgccg cacgcgcgtt ggcggttcgc
    13621 ggagcatcgt ccacacgggc agaccggagc ccaggccgtg cgcgtactgg gcgaggaccg
    13681 actcgagcgc ggccacctgc atcttgtcga tgcgcacggc ccgcgcgatc gggtgacgcc
    13741 ggagacgctc gatcagctcg ctccttccga gcacgatccc ggcctgcggc ccgccgagca
    13801 gcttgtcgcc cgagaacgcg atcaggtcgg cgccgtccgc gagcgcgccc gtcgcgctgg
    13861 gctcctccgc cgcggcgccc gggacgcgtt cgagaagacc cgagccgagg tcgtacagga
    13921 agggcacgtt cgcccgtcgc gcgatcccgg ccaggtcctt ggcgctcggc gtcgaggcga
    13981 accccaccac gcggtagttg ctggggtgca ccttgaggat cagcgccgtc ttgtccgaca
    14041 gcgccgaacg gtagtccgac gcgcgagtcc ggttcgtcgt accgacctcg acgagtcgcg
    14101 cccccgaggc cgccatgata tcggggatcc ggaactcgcc gccgatctcg atcagctcgc
    14161 cgcgcgagac caggacgccc ttgcctcgcg cgagcgaggc cagggcgagc agcaaggcgg
    14221 ccgcgccgtt gttcacgacc agcgcgtcct cggccccggt catcgacgtg agcagacgct
    14281 cggcccgggt cgaacgccgt tcgcgcgccc cggttccgcg gtcgacctcg agatcgacgt
    14341 agccctgtcc tgcccgcgcc gcggcggccg cggcccgtgc cgggagcggc gccctgccca
    14401 gacccgtgtg cagcacgacc cccgtcgcgt tgatcacgcg gacgaggccc gcggcggtcg
    14461 ccgacgtcag cccgagcgcc cgcgcgagga tctcgtcgtg gggcgggggc tcggtgccgc
    14521 cttcggcggc ctcgcggacc tgttcgagcg tgaccgacag cgcgtgcttg accagcggcc
    14581 ggccgaaggt gccgctcgcg cgccggcccg gttcggagcg cagcagcgcg tcgaccgagg
    14641 ggatccgtcg acggggttcg cggcccgcgg tcgtaatggc gttcccggtg cgttcgcgtt
    14701 cggcgcgggc catggtgagg ccagcctact accggccctc tcgccgcttc cgggggcgtt
    14761 cgcgatccgg ctcgctggtc gctgccgcac gtccgtggca ggatggaacc ggtggatacc
    14821 gaggaacgca ccgccttcac cgccgccgcc gagaactgga tggttcgggg cggaggggtc
    14881 gatatcgaga tcgttcccca ggggacgctc ggcaccgagg agatgatcct
//
LOCUS       DATBRK010000025         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1446596, whole genome shotgun sequence.
ACCESSION   DATBRK010000025 DATBRK010000000
VERSION     DATBRK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446596"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VIH70_00815"
     CDS             <1..801
                     /locus_tag="VIH70_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8114932.1"
                     /translation="TPPWFFYEAIILGGEAHPVRVPVDYETWDLDLDAIEAAITERTT
                     AIIVNSPNNPSGKIYPPETLRALADVLTAGSERIGHPIYLISDEAYSRIVFDDRSFTS
                     PTESYANSFLVYTYAKQLLTPGQRVGYIALPPEMPDRPQMREALQLGQLTYGGHAAPT
                     NVLQRAMGDLEGMSVDVKALQRRRDRVVEALRAYGYDLHSPEGTFYLLPTSPDPDDVA
                     FVERLAEDKVFVLPGTVVEMPGRFRISITGNDEMTERALPIFERAAKA"
     gene            809..2068
                     /locus_tag="VIH70_00820"
     CDS             809..2068
                     /locus_tag="VIH70_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEY8114933.1"
                     /translation="MPVELTEGDRVFLRGDRGEAAALAMRIIVEMAGVSGAERLIDVA
                     SAHVDGCLYHGIAGLAFAERLVAGGARVAVPTTLNVGALDLLHPDRYRGDAVTAANAR
                     RQMDAYVAMGCRPTWTCAPYQLPERPAFGQHVAWAESNAIVFCNSVLGARTDRYGDFI
                     DICASLTGRVPFAGLHRDEARRAGLVVTLEGISGRLLASDALYSVLGHLLGREAGTEV
                     PAIVGLPADTSEDRMKALGAAAASSGSVAMVHAVGITPEAPTLEAATGGEEARELRVT
                     TARLRAARDELGTDAPERLGAVSVGTPHASLAELERLTGLLGTDEVAVPLYVNVGRDV
                     LAKAEGGGLVPRLESMGVRVVSDTCTYISPVMDEVSGSVMTDSAKWAWYAPANLGVDV
                     VIGGLEECVRSAVAGRVLLDETLWAGA"
     gene            2061..2465
                     /locus_tag="VIH70_00825"
     CDS             2061..2465
                     /locus_tag="VIH70_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEY8114934.1"
                     /translation="MPEARTLVAGTAQGRALVLDEPLSFWGGVDPATGDIIDARHPQH
                     GANVAGRILVLPSGRGSSSSSSVLAEAIRAGTAPAAIVLGGADPILALGAIVARELFG
                     ATIPVVVMAASALRTDDLATVRADETAVELRG"
     gene            complement(2493..2891)
                     /locus_tag="VIH70_00830"
     CDS             complement(2493..2891)
                     /locus_tag="VIH70_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114935.1"
                     /translation="MSEAPSKSEPIISLDTSLIRVGRLDELKRAVGELVEFVRSNEPR
                     PIVYEVYLDNTESRMTVVQVHPDSASMEYHMTVAGPAFAGFAELVTLSTLDVYGKPSE
                     ELLELLRRKVQMLGEATVNVHDLQAGFARI"
     gene            complement(2947..>3219)
                     /locus_tag="VIH70_00835"
     CDS             complement(2947..>3219)
                     /locus_tag="VIH70_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114936.1"
                     /translation="GSSPTEVRKVIDTGAGSEPNIGASSSYGRAVTRVPDEAGTWYVD
                     VAALVSRFGSQLPPDVASNIEPIKTVVQGTSTSSSLSTYRLFIEIG"
BASE COUNT          455 a         1134 c         1142 g          488 t
ORIGIN      
        1 acgcctccgt ggttcttcta cgaggcgatc atcctcggcg gcgaggcgca cccggtgcgc
       61 gtcccggtgg actacgagac gtgggacctc gacctggacg cgatcgaggc cgcgatcacc
      121 gagcgcacca cggcgatcat cgtgaactcg cccaacaacc cgagcggcaa gatctacccg
      181 cccgagaccc tgcgtgccct ggccgacgtg ctgaccgccg gctccgaacg gatcgggcac
      241 ccgatctacc tgatctcgga cgaggcgtac tcgcggatcg tcttcgacga ccggtccttc
      301 acgagcccga ccgagtccta cgcgaactcg ttcctggtct acacgtacgc caagcagctg
      361 ctgacgcccg ggcagcgggt cgggtacatc gccctgccgc ccgagatgcc cgaccgtccc
      421 cagatgcgcg aggcgttgca gctcggccag ctcacgtacg gggggcacgc ggctccgacc
      481 aacgtcctgc agcgcgcgat gggcgacctc gaggggatga gcgtggacgt caaggcgctg
      541 cagcggcgcc gagaccgcgt cgtggaggcg cttcgcgcct acggctacga cctccactcg
      601 cccgagggca cgttctatct cctgccgacg agtccggacc cggacgacgt cgcgttcgtc
      661 gagcgcctcg ccgaggacaa ggtgttcgtc ctgcccggga ccgtcgtcga gatgcccggg
      721 cgcttccgaa tctcgatcac cggcaacgac gagatgaccg agcgggcgct cccgatcttc
      781 gaacgggcgg cgaaggccta ggccctcggt gccggtcgag ctcaccgagg gcgaccgcgt
      841 cttcctgcgg ggcgatcggg gcgaggccgc cgcgctcgcg atgcgcatca tcgtcgagat
      901 ggccggcgtc tccggcgccg agcggctgat cgacgtggcc tcggcgcacg tcgacgggtg
      961 cctgtatcac gggatcgcgg ggctcgcgtt cgccgagcgc ctggttgccg gcggcgccag
     1021 ggtcgcggtg cccaccacgc tgaacgtcgg cgccctcgac ctgctgcatc ccgaccggta
     1081 ccgcggggac gcggttaccg ccgcgaacgc ccgtcgacag atggacgcgt acgtcgcgat
     1141 gggctgccgg cccacgtgga cctgcgcgcc gtaccagcta cccgagcgtc cggcgttcgg
     1201 gcagcacgtc gcgtgggccg agtccaacgc gatcgtgttc tgcaactcgg tgctcggcgc
     1261 tcgcaccgac cggtacggcg acttcatcga catctgcgcc tcgttgacgg gccgggtgcc
     1321 gttcgccggg ctgcatcgcg acgaggctcg aagggcgggt ctcgtggtga cgctcgaggg
     1381 gatctcgggg cggctgctcg cgtcggacgc gctgtactcg gtgctcgggc acctgctcgg
     1441 ccgcgaggcc gggaccgagg tccccgcgat cgtcggcctg ccggcggaca cgtcggagga
     1501 ccggatgaag gcgctcggag ccgcggccgc ttcgtcgggc tcggtggcga tggtccacgc
     1561 tgtcggcatc accccggagg caccgacgct agaggccgcg accgggggcg aggaggctcg
     1621 cgagctccgg gtcacgacgg ctcgacttcg ggcggcccgg gacgagctcg gaaccgacgc
     1681 ccccgaacgg ctcggcgcgg tcagcgtcgg gacaccgcac gcctcgctcg ccgagctcga
     1741 acggctgacc gggctcctgg ggacggacga ggtcgcggtg cctctgtacg tcaacgtcgg
     1801 cagggacgtg ctcgcaaagg ccgaaggcgg agggctcgtg ccgcggctcg agtcgatggg
     1861 tgttcgggtc gtctccgaca cctgcaccta catctcgccc gtcatggacg aggtgagcgg
     1921 ctccgtcatg accgactccg cgaagtgggc ctggtacgcc ccggcgaacc tcggcgtcga
     1981 cgtggtgatc ggcgggctcg aggagtgcgt tcggtcggcg gttgccgggc gcgtcctgct
     2041 cgacgagacg ctgtgggccg gtgcctgagg cccgaaccct cgtcgcgggg acggcccagg
     2101 gccgggcgct cgtgctcgac gagccgctct cgttctgggg cggcgtcgac ccggccaccg
     2161 gcgacatcat cgacgcccgc cacccgcagc acggggcgaa cgtggccggg cggatcctgg
     2221 tgctgccgtc gggacgcggc tcgagctcct cctcgagcgt gctcgccgaa gcgatccgcg
     2281 ccggcacagc acccgccgcg atcgtccttg gcggggccga cccgatcctc gccctcggcg
     2341 cgatcgtggc tcgggagctc ttcggcgcca cgatccccgt ggtcgtgatg gccgcgtccg
     2401 ccttgcggac cgacgacctg gccaccgttc gggccgacga gaccgccgtc gagctgcggg
     2461 gctgaggccg aggctccggc ggctcgccgc ccctagatcc gagcgaaccc cgcctgtagg
     2521 tcgtggacgt ttaccgtcgc ctctcccaac atctggacct tcctccgaag gagctcgagc
     2581 agttcctcgc tcggcttgcc gtagacgtcc aacgtcgaca gggtgacgag ctcggcgaac
     2641 cccgcgaagg ccggacccgc gaccgtcatg tggtactcca tcgacgccga gtcgggatga
     2701 acttggacga cggtcatccg ggactcggtg ttgtcgaggt acacctcgta gacgatcggc
     2761 ctgggctcgt tcgaccggac gaactcgacc agttccccga cggccctctt cagctcatcg
     2821 agcctgccga cgcggatcaa ggacgtgtcg agcgagatga tcggctcgga cttgctcgga
     2881 gcctcggaca tgctcggagg gtacgaaccg cggcgcgagg aagcaaggtc cggatgtcgg
     2941 ggcctcttac ccgatctcga tgaagagccg gtaggtgctg agggaggacg aggtgctggt
     3001 cccctggacc acggtcttga tcggctcgat gttggaggcg acgtcgggtg ggagttgcga
     3061 cccgaacctc gagaccagcg ccgccacgtc cacgtaccac gtgcccgcct cgtccggtac
     3121 ccgagtgacg gcccgcccgt acgaggacga cgcgccgatg ttcggctcgc tccccgctcc
     3181 ggtgtcgatc accttccgga cctcggtggg gctggatcc
//
LOCUS       DATBRK010000026        16624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_148343, whole genome shotgun sequence.
ACCESSION   DATBRK010000026 DATBRK010000000
VERSION     DATBRK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148343"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VIH70_00840"
     CDS             <1..493
                     /locus_tag="VIH70_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="daunorubicin/doxorubicin resistance ABC
                     transporter ATP-binding protein DrrA"
                     /protein_id="HEY8114937.1"
                     /translation="IRDLRTEGTTLLLTTQYLDEADRLADSIAVIDGGRVIAEGTSDQ
                     LKARVGGEVLALRVADRSRAPDAAGAVLGLGPGGGNADNSTGEINVPVGTEGTAILTE
                     AVRRLDDQGIQLADVGLRRPTLDDVFLALTGHAAEEADGEQPSEDGGRSKRRDRKRER
                     EKV"
     gene            490..1335
                     /locus_tag="VIH70_00845"
     CDS             490..1335
                     /locus_tag="VIH70_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8114938.1"
                     /translation="MSAITETLRTPGRLANTVSDWWVITRRNLITYVRKPDLLVFSTI
                     QPVMFVLLFVFVFGGAFEMILPANLSYVDFLMAGIFVQTGIFAATQTGVGLADDLQKG
                     IIDRFRSLPMARSAVLAGRTAADTVSVGLQTVLMLIVAYLVGYRIHTSVWEALLAFGV
                     IVGVGYVFTWVSAYAGLTLKTIEAVQAALFTIVFPFVFVSSAFVPPATFPSWLQPIAQ
                     NNPVTIWVNLVRVLTLGGLVTESTSPLFSDVPSLWTLLWQSAAWMAGFLAFFYAMGIR
                     AYRRT"
     gene            1418..2203
                     /locus_tag="VIH70_00850"
     CDS             1418..2203
                     /locus_tag="VIH70_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8114939.1"
                     /translation="MQTPTTPSGSPAQQPGDQTCPNCGAPNGATVAFCYQCYQPFGAY
                     QTPPRQGGSPPDRGPWTPGMPAAPPAWTRTPSPFDAPARRRWDVGRIVAMVLVTLAAI
                     GLVNRWVGRDGSVALPEQFGGLGRMENAQTELMVDAFHRQLETMGVEGDIAMYGAGLP
                     TAGLVWIRDAAVPTTDAAFDEFATGFDSGIGAAGSLDGSRKTVETLDGVTYVCAPLES
                     YVSGTICMWQDQEVFWLLFDLSGGSFEAGKALAESAHDAASAA"
     gene            2296..3876
                     /locus_tag="VIH70_00855"
     CDS             2296..3876
                     /locus_tag="VIH70_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8114940.1"
                     /translation="MFGFEGENAREKVIVLFTMCFALAMAMLDNTVVNVALPTISREL
                     GAGVSELQWIVDGYVLVFASLLLTGGIAGDRYGRKKIFIAGLAIFTLASALCGLSQST
                     TQLIAARAFQGVGAALLMPGTLSIITVTFPPHERAKAIGLWAGVSGLALALGPTVGGL
                     MVEHLGWESVFFLNVPIGIVAIVAASRNVRESVSEVTRELDIPGLLLGSGALFCATYA
                     LIEANEKGWSDPLIVSTLVASAVLAVAFLLFERQSPKAMLPLRFFRIPAFSAGATVAF
                     SVSFGMFATFFFMSLYMQLIRGYSAFEAGLRFLPMTVMIIIVAPNAGRYASKHGSRIP
                     MTFGLILAGTGLLLLSRISIDTPYLLLAPVFVMMGIGIGATMAPMTAAVMNAVGPERA
                     GLGSATTNTAREVGGVFGIALLGTILTTRLNAVFAPALSGLGLAPTTQSAIEATAGHG
                     TLDPSQLSGLPPEQQGAVVAAFRESWMSGFRVSLVVAGLVLLVAAVVANRFIPGRAHA
                     REVTAAARVDESAPLELA"
     gene            3888..4133
                     /locus_tag="VIH70_00860"
     CDS             3888..4133
                     /locus_tag="VIH70_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEY8114941.1"
                     /translation="MEEDKDLIEHIEQLAHEEHELFDKESRGEASTKERARLKEIEGE
                     LEASYDLLRARRARRSAGLDPDVEPVTGARVVEEPTF"
     gene            complement(4174..4857)
                     /locus_tag="VIH70_00865"
     CDS             complement(4174..4857)
                     /locus_tag="VIH70_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY8114942.1"
                     /translation="MDERRIVVTKDGPYRVEGGVPLLRTAIVQTERGEPVAWDEGPAF
                     ETPGEVYELCRCGRSSTKPFCDSTHERIPFDGTETADRGPIAQRREAWEGEANHVLYD
                     DLSLCTHAGFCRNVRTGVWEMVEEADDPEVRSEFTAMVQRCPSGRLAFAVLPDPEPLE
                     PTFEPSVGVEPDASYWIRGGIPVVSEDETPYEVRNRQTLCRCGQSRNKPFCDGSHKQH
                     GFDDPAMPE"
     gene            complement(4914..5726)
                     /locus_tag="VIH70_00870"
     CDS             complement(4914..5726)
                     /locus_tag="VIH70_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEY8114943.1"
                     /translation="MSVTEFPELRIELGPAAEAIERTGRRLADLLTTVDEPDRGVRGL
                     EWTLGELIAHVAARSGRFAAYLSGAAVPEGEIAGIAEENRRDVRERRERPLADLVEEL
                     RSNVSSFVATTHGKLGADPLPWYSGVTLDVATASGLLLGELNVHGYDAAGTLGRPWAI
                     AGNDARTVVRAMATLAPWYVDPDATRGDRTTYRVVVRGGPAFRVRIDDGTASIEPTEG
                     DADCTIRAEPSALVLVLYGRISQWRGVARGRFVATGRRPWRALRFARSFLPP"
     gene            5822..7135
                     /locus_tag="VIH70_00875"
     CDS             5822..7135
                     /locus_tag="VIH70_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY8114944.1"
                     /translation="MAALAPPRPRLRVVAVTLLLAGAACTSTPAPGADRTTSEPTTAA
                     PRPTASATPTSETPTSTLVRSPNGGSLLVRGTYPRVASRCVRYHRPRLAARYPGTLSV
                     KRLDDGTLALTVTLPFEDYLKGIAEVPPSWPRAALEAQAIAARSYALATTGWEGAEGE
                     TLKTPICATTSCQVYRGIPVPFDPSVRRWFAAVRRTSGEVLLYGDRPADTVYFSTSNG
                     RTYGNDEVFGSAPLPYLRPVVERDDGASPVSHWRVRVPFDDVATFLHEAGEWPPQRTI
                     SSVGLEGSTVTVQGAGASRSMDLTTFRDAVNVWGPCLKPGRYPPSPLPVTLPSKWMTL
                     SSSGDAAVAVGRGWGHGAGMVQWGAYGKALRGLSASDILAYYYGGLRPQTYPEPGLIH
                     VQVVSGLTSIRVVPSGPGATLDGEELGSGRVTITGGDQLTVTTRP"
     gene            7132..8484
                     /locus_tag="VIH70_00880"
     CDS             7132..8484
                     /locus_tag="VIH70_00880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8114945.1"
                     /translation="MTRRTLLVAAVAVAVAVAFADSSIVVLALPELYARFDTTIEGVA
                     WVITSYNLVLAICALALVVVVHHARANLLLAAGTVLFLAASIACALADSIGFLIAARS
                     VQGLGGALLLAGSLPVLGALAGSTARGVAIWTLAGTFGAAVGPALGGVLTQLFDWRAI
                     FIFQAPVAGAALLATFGAHVAAILREGWRPSLWRTVPGNVALALLSGALVGALFLGVL
                     LVIDVFGLTPIQGAAVVSAIPAATLLARPLAAQLPVALAIASGAVLLAAGLIGLAFLP
                     SSHLGYTLSSFALCGFGLGLAVPGLTHAVLGGEGGIAPKATLTVGVRHLGLVLALAIG
                     APLLAGDLLPGADVAKLNATAVIIDGEIPGATKIPLALDIRDELERAPKGEIPDFAAA
                     FEEAGAGTDDAVRQVHEDLVETIERAVARSFRPSFLFCALLAALALVPILVFRRRWVA
                     "
     gene            8481..8936
                     /locus_tag="VIH70_00885"
     CDS             8481..8936
                     /locus_tag="VIH70_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114946.1"
                     /translation="MSRRTALGVLVALFVGAAGLIALEFANGAADAGALAIRDPCEPR
                     PAFPGEGIDATLQRIVSDGLDGAACELGTSREELVLSLAPGSGVEPVPWEDETIERAV
                     RAGLLASIDDAEQRGSLGGLLSVALRQVVEHAPVQWLIDGGQGIAGLLD"
     gene            8980..9435
                     /locus_tag="VIH70_00890"
     CDS             8980..9435
                     /locus_tag="VIH70_00890"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8114947.1"
                     /translation="MAVDRSYVDVNAAALDRLRAFVEKASDADLEHPMNDGWTVAAVL
                     AHIAFWDLRIVACIEAWKPDGTGPVPTYYDDAVDWINDSVKPLISALEPRVAARVTIE
                     AAEAADLAVAGLSDELFARNESLGGLVNPDRADHRLEHLDEIEQVLSRD"
     gene            complement(9459..9773)
                     /locus_tag="VIH70_00895"
     CDS             complement(9459..9773)
                     /locus_tag="VIH70_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114948.1"
                     /translation="MVRTPEQLWEPIARDQLGKRGVSTGTGFGTSEGLRVSGNIFAML
                     VRGELVVKLPRDRVDELVAAGAARRFDAGKGRPMKEWASVPASASRRWRGLVEEARAF
                     VG"
     gene            complement(9773..10255)
                     /locus_tag="VIH70_00900"
     CDS             complement(9773..10255)
                     /locus_tag="VIH70_00900"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8114949.1"
                     /translation="MVWATLRLIDACLPLAREQLEAAVPGTYGSILQTMRHLVEADTY
                     YLGHLTSDPAREIDPGRMGLGELRAEMEADERTWIELLAAELEPDGVVTDLDEEGYRR
                     DASIGVRIAQAIHHGTDHRSQICTTLTTLGLEPPLIDVWAFGDQTGRVIEVPPTSSPS
                     "
     gene            complement(10336..10707)
                     /locus_tag="VIH70_00905"
     CDS             complement(10336..10707)
                     /locus_tag="VIH70_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8114950.1"
                     /translation="MPDLESNKRTVVEFYELAFNGKQPEQAVEKYVGSQYLQHNPQAP
                     DGSEAFIGFVKAFPEASVDIRRVFADGDIVVTHSLLKFTADDRGTVAADFFRLEDGKV
                     VEHWDVLQPFPEESANPHPMF"
     gene            complement(10822..11277)
                     /locus_tag="VIH70_00910"
     CDS             complement(10822..11277)
                     /locus_tag="VIH70_00910"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8114951.1"
                     /translation="MDLDEVARAIIDGNRYMVLGTADEGGWPWVSPVYYAPSGYSELH
                     WVSSPEAQHSRNLAARPDLSIVVFDSQAPVGEGQGVYMSAVAEQPTGADLERGMEVFS
                     RVSVSHGAREWTVEDVRPPAALRLYRARVVEHWVLDPERRPDQRTPVWP"
     gene            complement(11298..12089)
                     /locus_tag="VIH70_00915"
     CDS             complement(11298..12089)
                     /locus_tag="VIH70_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8114952.1"
                     /translation="MDTATSTDGTTIAFDTRGEGPPLIVVGGALSDRRAAASLAELLA
                     PSFTVYAYDRRGRGDSGDTPPYAVEREVEDLQALVEHAGGTAFAFGHSSGAVLALEAA
                     LETPGIRKLVLYEPPFIVDDSRPPVPGDFVEHLRELTATGRRGEAVEYFMTTGVGAPA
                     EAIPSMRESPFWPSLEALAHTLWYDGLIMGDDMVGKPLSADRWSSVTIPALVIDGGAS
                     PPTLRNAAQQLADALPNARRLTMEGQTHEVDPALLAPVLTEFLTS"
     gene            complement(12162..13547)
                     /locus_tag="VIH70_00920"
     CDS             complement(12162..13547)
                     /locus_tag="VIH70_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8114953.1"
                     /translation="MTTPTSISIPKLRADVRGQVIGPDDAEYDQARAVFYGGFDLYPA
                     VIVRVADEVDIARAIALARETGLELAVRSGGHSVIGQGVSDGGIVIDLAGLRAIDVDA
                     EGRTAWAQTGLTALEFTTATHAHGLGVGFGDTGSVGVGGITLGGGVGYLVRKHGLTID
                     SLLAAEIVTADGELRHVDAEHEPDLFWAIRGGGGNFGVATRFRFALHPIDTVFGGMLM
                     LPATVDTIEGFMTEAEAAPEELSSIANVMPAPPMPFVPEELHGKLSIMALMTYAGDVE
                     AGQRALAPFRALAEPLADMLRPMPYPEVYPPEEEDYHPVATAHTMFVESIGRPEAETI
                     LEHLATSTAMMGVAQLRTLGGAMARVPVEATAFAHRRSKIMVNCAALYERPEEIEVHG
                     PWIESLATSLKQADDGAYVNFLGFDGEARIRDAYPGPTWDRLRRIKTRYDPTNLFRRN
                     QNIPPATEDAA"
     gene            complement(13700..14116)
                     /locus_tag="VIH70_00925"
     CDS             complement(13700..14116)
                     /locus_tag="VIH70_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8114954.1"
                     /translation="MRTNLTIDDVGGLLDEPLVAVLATLRPDGSVLLSPVWHEWRDGG
                     FNLWLAADDVKTRHLRRDPRATIVVAESVDPLRGVELRTNARLLDAGDLSDAVRIATR
                     YVGPEKGHEYVHEAGYESVIGRLEPGHLRVWDFRDE"
     gene            14221..15981
                     /locus_tag="VIH70_00930"
     CDS             14221..15981
                     /locus_tag="VIH70_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114955.1"
                     /translation="MRRRMAFACAVLAMATAISGTTALAGGNGGFRTSRPSMLTAVKK
                     GVTVTPLLTVGDELPSGYRFEAIPDGISVRPVGRGLTDLFVNHETSKVPFPFASATPS
                     AAAGQSDFDNAQVSQLTVNTAGRVIDGSFVIDSGGGFQRFCSNFLATSKEGFDQDVFF
                     TNEESPDYAIRQENAWPPPLSNTGAEENGVVLALDPETGDYDTIYGMGRFNHENNVAI
                     PGYDDLVVLSGDDTFTSGTLTLPPGPNPAASAPAQSQLYSYIASDTDALLADKGDLWA
                     FVSDNSSFTDYYDFAPGSTQSITGHFIKVPKNIATGKDTDGSELTSSDLGYPAPDGGW
                     QGDLRTANPVVPIDGPQWVLEYWSHLNHVFQFVRVEDLAYDKRPGMGNVVYFADTGRA
                     TSGDPGPGKSTNGRIWKMVLDPDDPTQVDSLSILLDGDDSAVQTLGEIHQPDNVETTQ
                     TGLLVTEDPGGNNQFPANSPATNATTARLWYIPFSSPGSAQVVAKIDQSADAGPTDVG
                     PAPTTDLSLGNWGSWETTGIVDASEAFGTGAYLINIQAHTLWIEKAPGPDTFLDANSD
                     PDFTFKREGGQLLLIRIPGI"
     gene            complement(16042..>16624)
                     /locus_tag="VIH70_00935"
     CDS             complement(16042..>16624)
                     /locus_tag="VIH70_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8114956.1"
                     /translation="LPNPRGSIGRGHAFAQAVIGDSGGMDFQDVMAGVDHCIAAGVAD
                     RERLGISGLSYGGYMAGWAVTHTDRFKAAVAMSVVSNYVSFHLTSEVWMYDEMILRGE
                     WNDPTSQYVERSPVTHAHRCRTPTLVIQGAEDRCTPVGQGWELANAIAAGGTEVELVV
                     YPREGHVPMERAHALDAIRRTQTWFNRHLRSVD"
BASE COUNT         2330 a         5959 c         5731 g         2604 t
ORIGIN      
        1 catccgcgac ctgcggaccg agggaaccac cctgctgctc acgacgcagt acctcgatga
       61 ggccgaccgc ctggcggact cgatcgccgt gatcgacggc ggccgggtga tcgccgaggg
      121 cacgtcggac cagctgaagg cgcgcgtcgg cggcgaggtg ctggccctgc gcgtggccga
      181 tcgcagccgc gcgccggacg cggcgggcgc cgtgctgggg ctcggacccg gcggcggcaa
      241 tgcggacaac agcaccggcg agatcaacgt gccggtcggc acggaaggga cggcgatcct
      301 gaccgaggcg gtccggcgac tggacgacca ggggatccag ctggccgacg tgggcctgcg
      361 tcgacccacg ctcgacgacg tcttcctggc cctcaccggg cacgcggccg aggaggcgga
      421 cggggagcag ccgtccgaag acggcggcag gtccaagcgc cgcgatcgca agcgagagcg
      481 agagaaggta tgagcgcgat caccgagacg ctccgcacgc ccggccgcct cgcgaacacg
      541 gtctcggact ggtgggtcat cacccggcgg aacctcatca cgtatgtgcg gaagccggac
      601 ctgctggtgt tctcgacgat ccagccggtg atgttcgtgc tgctgttcgt cttcgtgttc
      661 gggggcgcgt tcgagatgat cctccctgcc aacctgtcgt acgtcgactt cctgatggcg
      721 gggatcttcg tgcaaacggg gatcttcgcc gcgacgcaga cgggtgtcgg gctcgccgac
      781 gaccttcaga agggcatcat cgatcgcttc cggtcgctgc cgatggctcg ctcggcggtc
      841 ctggccggtc gcacggccgc cgacacggtc tcggtgggtc tccagacggt gctgatgctg
      901 atcgtcgcgt acctggtcgg ttatcggatc cacacgagtg tgtgggaggc gctgctcgct
      961 ttcggcgtga tcgtcggtgt gggatacgtg ttcacctggg tgtccgccta tgccgggctc
     1021 acgctgaaga cgatcgaggc ggtccaggcg gcgctgttca cgatcgtgtt cccgttcgtc
     1081 ttcgtgagct cggccttcgt cccgccggcg accttcccct catggctgca accgatcgcg
     1141 cagaacaatc cggtgacgat ctgggtgaac ctggtccgag tgctcacgct cggcggcctc
     1201 gtcaccgaga gcaccagccc gctgttctcg gacgtcccgt cgttgtggac cctgctctgg
     1261 cagtcggccg catggatggc gggcttcctg gccttcttct acgcgatggg catccgcgcc
     1321 taccgccgta cgtagtccgc cctcgcccct cccgagagcc ccgcggttca gccggcgttg
     1381 gcctttgccg atacctgctg cggaagggca tccgcgcatg cagacaccca cgacgccgag
     1441 cggttcgccg gcacagcagc cgggcgacca aacctgcccg aattgcggcg cccccaacgg
     1501 ggcgacggtc gcgttctgct accagtgcta ccaacccttc ggcgcgtacc agacgccgcc
     1561 ccggcagggg ggctcgccgc ccgaccgggg cccgtggacc ccggggatgc cggcggcgcc
     1621 tccggcttgg acgcggaccc ccagcccctt cgacgcaccg gcgcgacgcc gctgggacgt
     1681 tggccgcatc gtcgccatgg tgctcgtcac gctggccgcg atcggcctcg tgaaccggtg
     1741 ggtgggtcgc gacggctcgg tagcgctccc cgagcagttc ggcggcctgg gcaggatgga
     1801 gaacgcgcag accgagctca tggtggacgc cttccaccgg cagttggaga cgatgggggt
     1861 cgagggcgac atcgcgatgt acggggccgg cctgccgacc gccggccttg tgtggatccg
     1921 cgacgccgcg gtgccgacca ccgacgcggc cttcgacgag ttcgccacgg ggttcgactc
     1981 cgggatcggg gcggccggct cgctcgacgg gtcgcggaag accgtcgaga ccctcgacgg
     2041 cgtcacctac gtgtgtgccc cgctcgagag ctacgtgtcg ggcacgatct gcatgtggca
     2101 ggatcaggag gtcttctggc tcctgttcga cctatcggga ggctcgttcg aggcgggcaa
     2161 ggcccttgcc gaaagcgccc acgacgccgc ctccgcggcc tagcgcgggc ctgggaacat
     2221 ctcgccatcg ggctcggttg tcgcctgagg ccggtacagt gaccggaacg ccgccgatac
     2281 ctggaggtcc aacgcatgtt cggtttcgag ggcgagaacg cacgcgagaa ggtcatcgtc
     2341 ctgttcacga tgtgtttcgc gctcgcgatg gcgatgctcg acaacacggt cgtcaacgtg
     2401 gcgctcccca cgatcagccg cgaactgggc gccggcgtca gcgagctcca gtggatcgtc
     2461 gacgggtacg tcctggtctt cgcctcgctg ctgctgacgg gcggcatcgc cggcgaccgc
     2521 tacgggcgca agaagatctt catcgccggc ctcgcgatct tcacgctcgc ctccgcgctc
     2581 tgcggcctgt cccagagcac gacgcagctg atcgcggcgc gcgcgttcca gggcgtcggt
     2641 gcggccctgc tcatgcccgg cacgctgtcg atcatcacgg tcaccttccc gccccacgag
     2701 cgggccaagg cgatcggcct gtgggccggc gtgtcgggcc tcgcgctcgc gctgggcccc
     2761 accgtcggcg gcctgatggt cgagcacctc ggctgggagt cggtgttctt cctgaacgtc
     2821 cccatcggga tcgtggccat cgtcgcggcc tcgcgcaacg tccgcgaatc ggtctccgag
     2881 gtcacccgcg agctggacat ccccgggctg ctcctgggtt cgggcgcgct gttctgcgcg
     2941 acctacgccc tgatcgaggc caacgagaag ggctggagcg acccgctgat cgtcagcacg
     3001 ctggtcgcct cggccgtcct ggcggtcgcg ttcctgctgt tcgagcggca gagtcccaag
     3061 gccatgctgc ccctgcggtt cttccgcatc cccgcgttct ccgcgggcgc gacggtcgcg
     3121 ttcagcgtct cgttcggcat gttcgcgaca ttcttcttca tgtcgctgta catgcagctg
     3181 atccgcgggt acagcgcgtt cgaggccggc ctccggttcc tgccgatgac cgtcatgatc
     3241 atcatcgtcg cgcccaacgc cggtcgctac gcctccaagc acggctcgag gatcccgatg
     3301 acgttcgggt tgatccttgc gggcaccggc ctcctgcttc tctcgcgcat ctcgatcgac
     3361 acgccgtacc tgctgctggc gccggtcttc gtgatgatgg gcatcgggat cggcgccacg
     3421 atggcgccga tgacggccgc ggtgatgaac gcggtgggcc ccgaacgcgc gggcctcggt
     3481 tccgccacga cgaacaccgc cagggaggtc ggcggcgtgt tcggcatcgc gctgctgggc
     3541 acgatcctca ccacgaggct caacgccgtg ttcgctcccg cactgagcgg gctcggcctc
     3601 gcgccgacca cgcaatccgc gatcgaggcg acggccggac acggcacgct ggatccctcg
     3661 cagctgtccg ggctcccgcc cgagcagcag ggtgcggtgg tcgcggcctt ccgcgaatcc
     3721 tggatgagtg gcttccgcgt ctcgctcgtc gtggccggcc tggtgctgct ggtcgcagcg
     3781 gtggtcgcca accggttcat ccccggccgt gcccacgctc gcgaggtcac cgcggccgcg
     3841 cgggtggatg agtcggcccc cctcgaactg gcctaggctt cccctcgatg gaggaggaca
     3901 aggacctcat cgagcacatc gagcagctcg cccacgagga gcacgagctg ttcgacaagg
     3961 agtcccgcgg cgaggcctcc accaaggagc gcgcccgtct caaggagatc gagggtgagc
     4021 tcgaggcctc ctacgacctc cttcgcgctc ggcgggcccg gcgatcggcg gggctggatc
     4081 ccgacgtcga gcccgtgacc ggcgcgcggg tcgtcgagga accgaccttc tagcgcccgg
     4141 gggacgagct ccggagcacg gcgaggggag cgctcactcc ggcatcgccg ggtcgtcgaa
     4201 cccgtgctgc ttgtgcgatc cgtcgcagaa cggcttgttg cgcgactgcc cgcaccggca
     4261 cagggtctga cggttgcgca cctcgtacgg ggtctcgtcc tccgaaacga cggggatccc
     4321 gccgcggatc cagtacgacg cgtcgggctc gaccccgacc gagggctcga acgtcggctc
     4381 gaggggctcg gggtcgggca gcaccgcgaa cgcgagccgc cccgacgggc atcgttgcac
     4441 catcgcggtg aactcgctcc gcacctccgg atcgtccgcc tcctcgacca tctcccagac
     4501 gccggtccgg acgttgcggc agaacccggc gtgggtgcag agcgacagat cgtcgtacag
     4561 gacgtggttc gcctcgccct cccacgcctc gcggcgctgg gcgatcgggc cgcggtcggc
     4621 ggtctcggtg ccgtcgaacg ggatccgttc gtgcgtcgag tcgcagaagg gcttggtcga
     4681 cgaccggccg cagcgacaga gctcgtatac ctcgcccggg gtctcgaacg cggggccctc
     4741 gtcccacgcg accggctcgc cgcgctcggt ctgcacgatc gccgtgcgca gcaaggggac
     4801 cccgccctcg acgcggtacg ggccgtcctt ggtgacgacg atcctgcgct cgtccacccg
     4861 tggagtctaa acggctcttc ccggaccgct cgcctcgagg ggagccggcc ggctcagggg
     4921 gggaggaacg accgtgcgaa ccgcagcgcc cgccacgggc ggcgccccgt cgcgacgaac
     4981 ctgcccctcg cgacccctcg ccattgcgag atgcgcccgt acaacacgag gaccagcgcc
     5041 gagggctcgg cccggatcgt gcagtccgca tcgccctcgg tgggctcgat cgaggccgtg
     5101 ccgtcgtcga tccgaacccg gaaggcgggc cccccgcgga ccacgacccg gtacgtcgtg
     5161 cggtcgccgc gcgtcgcgtc ggggtcgacg taccaggggg cgagcgtcgc catggcgcgg
     5221 accaccgttc gggcgtcgtt gcccgcgatc gcccaggggc gcccgagggt cccggccgcg
     5281 tcgtagccgt gcacgttcag ctcgccgagc aacagcccgg acgcggtcgc gacgtccagc
     5341 gtgacccccg agtaccacgg caacgggtcc gcgccgagct tgccgtgggt ggtggcgacg
     5401 aagctcgaga cgttcgaccg cagctcctcg accaggtcgg cgagggggcg ttcgcgccgt
     5461 tcgcggacat cccgccggtt ctcctcggcg atgccggcga tctcgccctc ggggaccgcc
     5521 gcccccgaca ggtacgccgc gaaccgaccg gaccgggccg cgacgtgggc gatgagctcg
     5581 ccgagggtcc actccaggcc gcggacgccg cgatcgggct cgtcgaccgt cgtgagcagg
     5641 tcggcgagcc tccggcccgt ccgttcgatc gcctcggcgg cgggcccgag ctcgatccgg
     5701 agctccggga actcggtgac gctcatggag tcgcgctccg tgggacggac ttccgcatgc
     5761 cccgatcctc gctcctggac gccccgatcg gcaaaccggg gcgacctgtt agcgtgctcg
     5821 ggtggccgcg ctcgcacctc cacgtccgcg gctccgcgtg gtcgcggtca cgctgctcct
     5881 cgcgggcgcg gcgtgcacct cgacgccggc acccggggcg gaccgaacga catccgaacc
     5941 cacgaccgct gcgccgaggc ccaccgcgtc cgcgacaccc acgtcggaaa cgcccacgtc
     6001 caccctcgtc cgctcgccga acggcggctc gctcctggtt cgcggcacct acccgcgagt
     6061 cgcatctcga tgcgttcgat accaccggcc ccggctcgcg gcccgctacc cggggacgct
     6121 ctcggtcaag cgcttggacg acggcacgct cgcgctcacg gtgacgctgc cgttcgagga
     6181 ctacctgaag ggcatcgccg aggtcccgcc gtcctggccg cgcgcggcgc tcgaggcgca
     6241 ggcgatcgcg gcgcgcagct acgcgctcgc gaccaccgga tgggagggcg ccgagggcga
     6301 gacgctcaag acgccgatct gcgcgacgac ctcgtgccag gtgtaccgcg ggatccccgt
     6361 gccgttcgac ccgagcgtcc ggcggtggtt cgccgccgtc cggcgcacgt cgggcgaggt
     6421 cctcctctac ggagaccgcc ccgccgacac cgtctacttc tccacctcga acggccggac
     6481 gtacgggaac gacgaggtgt ttggcagcgc gcccctgccc tacctgcgcc cggtggtcga
     6541 acgcgacgac ggcgcgtcgc ccgtgtcgca ttggcgcgtc cgagtgccgt tcgacgacgt
     6601 ggcgacgttc ctccacgagg ccggcgagtg gccaccgcag cggacgatct cgagcgtcgg
     6661 tctggagggc tcgacggtca cggtccaggg cgcgggtgcg tcgcgctcga tggacctgac
     6721 cacgttccga gatgccgtca acgtctgggg accctgcctg aagcccggtc gctacccgcc
     6781 ctccccgctc cccgtgacgc tcccgtcgaa gtggatgacg ctctcctcga gcggggacgc
     6841 cgcggtcgcc gtcggacgcg gctgggggca cggcgccggg atggtgcagt ggggcgcgta
     6901 cggcaaggcg ctccgcgggc tctcggcctc ggacatcctc gcctactact acggggggct
     6961 gcggccgcag acctatcccg aacccggtct gatccacgtc caggtcgtga gcggcctcac
     7021 ctcgatccgc gtcgtgccgt ccggacccgg cgcgacgctc gacggcgagg agctcggctc
     7081 cggacgcgtg acgatcaccg gcggcgacca gctcaccgtg accacgcggc cgtgacccgt
     7141 cggacgctgc tggtcgcggc ggtcgcggtg gccgtcgcgg tggcgttcgc cgattcgtcg
     7201 atcgtcgtgc tcgcgctgcc cgagctgtac gcgcgcttcg acaccacgat cgagggcgtg
     7261 gcgtgggtga tcacctcgta caacctcgtg ctggcgatct gcgcgctcgc gctcgtcgtc
     7321 gtcgtgcacc acgcacgggc caacctgctt ctcgccgccg gcaccgtcct gttcctcgcc
     7381 gcgtcgatcg cctgcgcgct cgcggactcg atcgggttcc tgatcgccgc tcgctcggtc
     7441 cagggtctcg gcggggcgct gctgctcgcg ggttccctgc cggtcctggg cgcgctcgcc
     7501 ggctcgaccg cgcgcggcgt cgcgatctgg acgctggccg gcacgttcgg cgcggcggtg
     7561 gggccggcgc tgggcggcgt gctgacccag ctgttcgact ggcgcgccat cttcatcttc
     7621 caggcccccg tcgccggcgc ggcgctgctc gcgacgttcg gggcgcacgt cgccgcgatc
     7681 ctgcgagagg ggtggcggcc gagcctgtgg cggacggtgc cggggaacgt cgcgctggcg
     7741 ttgctctcgg gggccctcgt gggggcgctg ttcctcggcg tcctgctcgt gatcgacgtg
     7801 ttcgggctca ccccgatcca gggggccgcg gtcgtgagcg cgatcccggc cgcgaccctg
     7861 ctcgcgcggc ccctcgcggc ccagctcccg gtcgcgctcg cgatcgcctc gggcgccgtg
     7921 ttgctcgcgg ccgggttgat cgggctcgcg ttcctgccgt ccagccatct gggatacacg
     7981 ctctcgtcgt tcgcgctgtg cgggttcggg ctcgggctgg ccgtaccggg tctgacccac
     8041 gcggtgctcg ggggtgaggg cgggatcgcg ccgaaggcga cgctcaccgt cggcgtccgg
     8101 catctcgggc tggtgctcgc gctcgcgatc ggggcgccgt tgctcgccgg cgacctcctg
     8161 cccggcgccg acgtcgcgaa gctgaacgcc acggccgtga tcatcgacgg cgagatcccg
     8221 ggggcgacga agatccccct ggcgctggac atccgcgacg agctcgagcg cgcgccgaag
     8281 ggggagatcc cggacttcgc ggccgcgttc gaggaggccg gtgcggggac cgacgacgca
     8341 gtgcgacagg tgcacgagga cctcgtcgag acgatcgaac gcgccgtcgc caggagcttc
     8401 cgaccgtcgt tcctgttctg cgcgctgctc gcggcgctcg cgctcgtgcc gatcctcgtg
     8461 ttccggaggc ggtgggtggc gtgagccgcc gaacggcgct cggggtgctg gtcgcgctgt
     8521 tcgtcggggc cgccggcctg atcgcgctcg agttcgcgaa cggcgccgcc gacgccgggg
     8581 cgctcgcgat ccgcgacccg tgcgagcctc gccccgcgtt ccccggcgag ggcatcgacg
     8641 cgacgttgca gcgcatcgtg tccgacgggc tcgacggggc ggcctgcgag ctcggcacga
     8701 gccgcgagga gctcgtactc tccctcgcgc cgggctccgg cgtcgagccg gtcccgtggg
     8761 aggacgagac gatcgagcgc gccgtccgcg ccggcctcct cgcgtcgatc gacgacgccg
     8821 aacagcgagg gtcgctgggc ggtctcctgt ccgtggccct ccggcaggtc gtcgagcacg
     8881 cgccggtcca gtggctgatc gacggcggcc aggggatcgc gggactgttg gactagggtt
     8941 ggctcgcggc gagtacgcga cgaaggggag gaggacgtcg tggcggtcga tcgatcgtac
     9001 gtggatgtga acgcggcggc gctcgacagg ctgcgagctt tcgtcgagaa ggcgagcgac
     9061 gccgatctcg agcatccgat gaacgacggg tggacggtcg ccgcggtcct ggcccatatc
     9121 gcgttctggg acctgcggat cgtcgcgtgc atcgaggcct ggaagcccga cggaaccggg
     9181 cccgtcccca cctactacga cgacgcggtg gactggatca acgactccgt caagccgctg
     9241 atctccgcgc tcgagccgcg ggtggccgct cgggtgacga tcgaggccgc cgaggcggcc
     9301 gatctcgcgg tggccgggct gtcggacgag ctcttcgcga ggaacgagtc cctcggtggg
     9361 ctcgtcaatc ccgaccgcgc ggatcaccgg ctcgaacacc tggacgagat cgaacaggtc
     9421 ctgtctcgcg actagggcgt ccgagccgcc cgcgagcctc agccgacgaa ggccctcgcc
     9481 tcctcgacga gccccctcca tcggcgggac gcgctcgccg ggaccgacgc ccactccttc
     9541 atcggacgcc ccttccccgc gtcgaagcgt cgggccgcgc cggcggcgac gagctcgtcg
     9601 acgcgatccc tggggagctt cacgacgagc tcgcctcgga cgagcatcgc gaagatgttc
     9661 ccggagaccc gcagcccctc gctggtcccg aaccccgtgc cggtggacac gccgcgtttc
     9721 ccgagttgat ccctcgcgat cggctcccac agctgctcgg gggttcgaac catcagcttg
     9781 gcgaggacgt ggggggaacc tcgatgacgc gtcccgtctg gtcgccgaac gcccagacat
     9841 cgatgagtgg cggttcgagt ccgagcgtcg tcagggtcgt gcagatctgg ctccgatgat
     9901 cggtcccgtg gtggatcgcc tgggcgatcc ggacgccgat cgaggcgtct cgccgatagc
     9961 cctcctcgtc gaggtccgtc acgacgccgt ccggttcgag ctccgcggca aggagctcga
    10021 tccacgtccg ttcgtcggcc tccatctcag ccctcagctc cccgagaccc atccgacccg
    10081 gatcgatctc gcgagcagga tcgctcgtca gatgcccgag gtagtacgtg tctgcttcca
    10141 cgaggtggcg catcgtctgc aggatcgatc cgtaggtccc gggcacggcc gcctccagct
    10201 gttctcgagc cagtgggagg caggcatcga tcaaccgcag cgtcgcccag accatggtgt
    10261 gcgaaagcgt cgtcgaggag cgaacgagtc atgacgacga gcaggatagt cgcccgcgga
    10321 cccctcgttg cccgactaga acatcgggtg cgggttcgcg ctctcctcgg ggaacggctg
    10381 tagcacgtcc cagtgctcca cgaccttgcc gtcctcgaga cggaagaagt cggcagcgac
    10441 ggtacctcga tcgtcggccg tgaacttcaa caggctgtgc gtgacgacga tgtccccgtc
    10501 ggcgaagacg cgtcggatgt cgacgctcgc ctcggggaac gccttcacga acccgatgaa
    10561 cgcctccgag ccatcggggg cctgtgggtt gtgctgcagg tactgcgacc ccacgtactt
    10621 ctcgaccgcc tgctcgggct gcttgccgtt gaacgccaac tcgtagaact cgactaccgt
    10681 tcgcttgttg ctctcgaggt cgggcatctg catcccccct tggacagacc cgatcggcag
    10741 ccctacctgg tttgcaacga acgctgatgc tcacccattc cccgccctgc cgcatcttcg
    10801 tcgagggaga gcttccgcac gctacggcca gacgggcgtc cgctgatcgg gccggcgctc
    10861 cgggtctagc acccagtgtt cgactacgcg cgcccggtac aggcgcaacg ccgccggcgg
    10921 ccggacatcc tcgaccgtcc actccctcgc gccgtgcgag accgagacgc gggagaagac
    10981 ctccatgccg cgctcgagat cggcgcccgt cggctgctcg gcgacggccg acatgtacac
    11041 gccctgcccc tcgccgaccg gtgcctgcga gtcgaagacc acgatgctca ggtccggacg
    11101 ggccgcgagg ttgcgagagt gctgcgcctc cggcgaggag acccagtgga gctccgagta
    11161 tcccgacggc gcgtagtaga cgggcgagac ccacggccat ccaccctcgt cggcggtccc
    11221 gagcaccatg taccggttgc cgtcgatgat ggcccgcgcg acctcgtcga gatccatcgt
    11281 ccacctccga tcgggactca gctcgtgagg aactccgtca gcaccggcgc gagcagcgcc
    11341 gggtccacct cgtgggtctg gccctccatc gtgaggcggc gggcgttcgg gagggcgtcg
    11401 gcgagttgct gcgcggcgtt ccgcagcgtc ggcgggctcg ccccgccgtc gatcaccaac
    11461 gccgggatcg tgaccgatga ccaccgatcg gcggacagcg gcttgccgac catgtcgtcg
    11521 cccatgatca acccgtcgta ccacagcgtg tgcgcgaggg cctccagcga cggccagaac
    11581 ggtgactccc tcatcgacgg gatggcctcg gccggcgcgc cgacgcccgt ggtcatgaag
    11641 tactcgaccg cctcgccccg gcgaccggtc gcggtcaact ccctgaggtg ctccacgaag
    11701 tcgcccggca ccggcgggcg cgagtcgtcg acgatgaagg gaggctcgta gagcacgagc
    11761 ttcctgatgc ccggggtctc gagcgcggcc tcgagcgcga gcaccgcgcc cgacgagtgg
    11821 ccgaacgcga acgcggtccc gcccgcgtgc tcgacgagcg cctgcaggtc ctcgacctcg
    11881 cgttcgaccg cgtacggggg cgtgtcgccg ctgtcgccac ggccgcggcg gtcgtaggcg
    11941 tagaccgtga agctcggggc gaggagttcg gccaggctcg cggcggcacg gcgatcgctc
    12001 agcgcgccgc ccaccacgat cagcggtggc ccctccccac gcgtgtcgaa cgcgatcgtc
    12061 gtcccgtccg tcgaggtcgc cgtgtcgatg tcgctccctt tcttgccgtc ttcttgccgt
    12121 cgtcgatcct cttccctacc acatgaccgt cgtggtgagg ctcacgccgc gtcctcggtg
    12181 gccggcggga tgttctggtt gcgccggaac aggttcgtcg ggtcgtagcg cgtcttgatc
    12241 ctcctgagcc ggtcccacgt cggtccgggg taggcgtcgc ggatccgcgc ctcgccgtcg
    12301 aaacccagga agttgacgta cgcgccgtcg tccgcctgct tgagggaggt ggcgaggctc
    12361 tcgatccacg gcccgtgcac ctcgatctcc tcggggcgtt cgtagagtgc ggcgcagttc
    12421 accatgatct tgctccggcg gtgcgcgaat gcggtcgcct cgaccggcac gcgcgccatc
    12481 gcaccgccga gcgtccgcag ctgcgcgacg cccatcatcg cggtcgaggt cgcgagatgc
    12541 tccaggatcg tctcggcctc gggacgcccg atcgactcga cgaacatcgt gtgcgcggtg
    12601 gcgaccgggt ggtagtcctc ctcctccggc gggtagacct cggggtacgg catcggccgg
    12661 agcatgtcgg cgagtggctc ggcgagcgct cggaacggcg ccagggctcg ctgaccggcc
    12721 tcgacgtcgc cggcgtacgt catgagcgcc atgatcgaga gtttgccgtg cagctcctcg
    12781 ggcacgaacg gcatcggcgg cgccggcatc acgttggcga tcgacgagag ctcctcgggc
    12841 gcggcctcgg cctcggtcat gaagccctcg atcgtgtcga ccgtcgcggg gagcatgagc
    12901 atgccgccga acacggtgtc gatcggatgc agggcgaacc tgaaccgggt cgcgacgccg
    12961 aagttccccc cgccgccgcg gatcgcccag aacaggtcgg gttcgtgctc ggcgtcaaca
    13021 tggcggagct cgccgtccgc agtcacgatc tccgcggcca gcagactgtc gatcgtgagg
    13081 ccgtgcttgc ggacgaggta gcccacgccg cctccgagcg tgatcccccc gacccccacc
    13141 gaacccgtgt cgccgaagcc gacccccagg ccgtgagcgt gggtcgcggt ggtgaactcg
    13201 agcgcggtca ggccggtctg cgcccacgcc gtgcgcccct cggcgtcaac gtcgatcgcg
    13261 cgcaggccgg cgaggtcgat cacgatcccg ccgtcggaca ccccctgccc gatcacgctg
    13321 tggccgccgc tgcgcacggc gagctccagc ccggtctcgc gcgccagcgc gatcgcgcgg
    13381 gcgatgtcca cctcgtcggc gacccggacg atcacggcgg ggtacaaatc gaacccgccg
    13441 tagaagaccg cgcgagcctg gtcgtactcg gcgtcgtcgg gtccgatcac ctgccccctc
    13501 acgtcggccc ggagcttggg gatcgagata ctggtcgggg tggtcatcga tggcctcctc
    13561 cctcggggtg ctacgtgtct cgaccgcggt acggacgcga actcatcggt cgagacgggt
    13621 tcacgctcgt actagttggc aacccgggtt gcgtttcctc ttcttcccgg cggcatacgc
    13681 ctgccggtac gtgatcggcc tactcgtctc ggaaatccca gacgcgcagg tggccgggtt
    13741 cgaggcggcc gatcacggat tcgtagcccg cctcgtgtac atactcgtgg cccttctccg
    13801 gacccacgta ccgggtggcg atgcggacgg cgtcggacag atcgcccgcg tcgagcagcc
    13861 gggcgttcgt gcggagctcc acgccacgga gcgggtcgac cgattcggca acgacgatcg
    13921 tggcgcgtgg atcgcgacgg agatgccggg tcttgacgtc atcggcggcg agccacaggt
    13981 tgaacccgcc gtcccgccac tcgtgccaca ccggcgagag caggaccgag ccgtcggggc
    14041 gaagggtcgc caggaccgcg acgagcggct cgtccaggag gccgcccacg tcgtcgattg
    14101 tcaggttcgt gcgcacggct cacgataacc ggccggggcg ggactacgtc ggccgcgggt
    14161 tgcccgcccc aaggggggtc tgctaggact ggtcttgtcg ccgatcccct aggaggaaac
    14221 atgcgacgac gcatggcatt cgcctgcgcc gtgctcgcga tggcgaccgc catctcgggc
    14281 acgacggcgc tcgcgggcgg caatggcggc ttcaggacct cgaggccgtc gatgctcacg
    14341 gccgtcaaga agggtgtcac cgtgacgccg ctcctgaccg tgggggacga actgccgagc
    14401 gggtatcggt tcgaggccat cccggacggg atctcggtac gtccggtcgg ccgtgggctg
    14461 acggacctgt tcgtgaacca cgagaccagc aaggtgccgt tcccgttcgc ctcggccacc
    14521 ccgagcgcgg ccgccggcca gagcgacttc gacaacgcgc aggtgagcca gctcaccgtg
    14581 aacacggccg ggcgcgtgat cgacggctcg ttcgtcatcg acagcggtgg tggcttccag
    14641 cgcttctgct cgaatttcct cgccacgagc aaggaggggt tcgatcagga cgtcttcttc
    14701 acgaacgagg agtccccgga ctacgcgatc cggcaggaga acgcatggcc gccgcccctc
    14761 agcaacacgg gagccgagga gaacggcgtc gtcctcgcgc ttgatccgga gaccggcgac
    14821 tacgacacca tctacggcat gggccgcttc aaccacgaga acaacgtcgc gatcccgggc
    14881 tacgacgacc tggtggtcct ctcgggcgac gacacgttca ccagcgggac gctgacgctc
    14941 ccgccgggcc cgaaccccgc ggcgtccgca ccggcccagt cgcagctcta ctcgtacatc
    15001 gcctcggaca cggacgcgtt gctcgccgac aagggcgacc tctgggcttt cgtctccgac
    15061 aactcgagct tcaccgatta ctacgacttc gcgcccggct ccacccagtc gatcaccggt
    15121 cacttcatca aggtgccgaa gaacatcgcc accgggaagg acaccgacgg ctcggagctg
    15181 acgtcatccg atctcgggta ccccgccccc gatggcggct ggcagggtga ccttcgtacg
    15241 gcgaacccgg tcgtcccgat cgacggcccg cagtgggtgc tcgagtactg gagccacttg
    15301 aaccatgtct tccagttcgt ccgcgtggag gacctcgcgt acgacaagcg gcccgggatg
    15361 ggcaacgtcg tctacttcgc cgacacggga cgcgcgacgt ccggtgaccc gggaccagga
    15421 aagtcaacca acggccgcat ctggaagatg gtcctcgatc ccgacgaccc gacgcaggtc
    15481 gactcgctct ccatcctcct ggacggagac gacagcgccg tccagacgct gggcgagatc
    15541 catcagccgg acaacgtcga gaccacgcag acgggcctcc tcgtcaccga ggatcccggc
    15601 ggcaacaacc agttccccgc caactccccg gctacgaacg cgaccacggc gcgcctgtgg
    15661 tacatcccgt tctcgtcacc tggctctgcg caggtggtcg ccaagatcga ccagtcggcc
    15721 gacgcgggcc cgaccgatgt cggtcccgcc ccgaccaccg atctctcgct cggcaactgg
    15781 ggctcgtggg agacgaccgg catcgtcgac gcctccgagg cgtttggaac cggcgcgtat
    15841 ctgatcaaca tccaggcgca cacgctctgg atcgagaagg cgccgggtcc ggacacgttc
    15901 ctcgacgcga acagcgatcc ggacttcacg ttcaagcgcg aaggaggaca gctgctgctg
    15961 atccgcatcc ccgggatcta gccgcggcgc acgacgcaag ggggcgccct tcggggcgtc
    16021 ccttcgcgtc caaaaaggcg ttcagtcgac cgaacgcagg tggcggttaa accacgtctg
    16081 cgtgcgccgg atcgcgtcga gcgcgtgcgc gcgctccata ggcacgtggc cctcgcgggg
    16141 gtagacgacg agctcgacct cggtgccgcc ggcggcgatc gcgttggcga gctcccaccc
    16201 ctgaccgacc ggcgtacacc gatcctccgc accctggatc acgagggtcg gcgtccggca
    16261 ccggtgggcg tgggtcacgg gcgagcgctc gacatactga ctcgtcgggt cgttccactc
    16321 gccccgcagg atcatctcgt cgtacatcca gacttccgac gtcaggtgga acgagacgta
    16381 gttcgaaacc accgacatcg cgaccgccgc cttgaatcgg tcggtgtgcg tcaccgccca
    16441 tccggccatg tagccgccgt aggacaggcc cgagatgccg agacgctcgc ggtcggcgac
    16501 gcccgcggcg atgcagtggt cgacgccggc catcacgtcc tggaaatcca tcccgcccga
    16561 gtcgccgatc accgcctgcg cgaacgcgtg cccgcggccg atgctccctc gcgggttcgg
    16621 cagc
//
LOCUS       DATBRK010000027         6785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_581289, whole genome shotgun sequence.
ACCESSION   DATBRK010000027 DATBRK010000000
VERSION     DATBRK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581289"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VIH70_00940"
     CDS             complement(<1..587)
                     /locus_tag="VIH70_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8114957.1"
                     /translation="MIAILGGLVAAAMWAASALCISRSTRMIPPVAVLGWVLAIGSVI
                     SAPFALAQGVPDGLGREQVVLLVVTAVGNTTGLLLVYSSLRVGKVGVVAPITSAQGAA
                     AAVIAVIAGEQVATGAGIALAAIVVGVVLSSMSRTDDSGSDRREGLAVTLAIGAAVFF
                     GLGLFAMGRLSEDIPIAWIIFPSRTIAVLLVTVPLA"
     gene            complement(660..1154)
                     /locus_tag="VIH70_00945"
     CDS             complement(660..1154)
                     /locus_tag="VIH70_00945"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEY8114958.1"
                     /translation="MSRTEVLSLANARLGSAGDLADWLEAEGALEPEDRAEVSLRLAE
                     FVTLRASIRELLDASIGGGTFPAAAVEHINEASARVPRVRRLAPDGAVDTSLSAGPTH
                     LLLARISWSAIELLGDPDRPARRCGACGSYFEATRSDRVWCSNACGNRTRVARHHARR
                     RVPA"
     gene            complement(1249..2082)
                     /locus_tag="VIH70_00950"
     CDS             complement(1249..2082)
                     /locus_tag="VIH70_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY8114959.1"
                     /translation="MPELPEVQALAERLTEAVGGSAFAAADLVQFSSLKTVTPRASEL
                     AGRILERVGRRGKYLVLELGGPRVLVHLSQGGRVDVEDPPKSTKPRGSVVRFRFEDRP
                     SVLVKEFGRERKAGWWVLAEGDEGPLAKLGPEPDSPEFERLVLEGEDGRRVHTILRDQ
                     RTVAGIGRGYSDDILHRAKLSPYASLASLEPGQRKALLAAVGSVLDEALAAERRRTGG
                     LQTKIGDHFTVHGRYGEPCPVCGDDLRRVSYESHEVTYCPTCQTGGKVLADRRLSRLI
                     R"
     gene            complement(2120..3517)
                     /locus_tag="VIH70_00955"
     CDS             complement(2120..3517)
                     /locus_tag="VIH70_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8114960.1"
                     /translation="MSYPLEPGPEQMRAMGEAALGYLVEFVQSQDEAAAMDVDGAIEA
                     ARSVRGAPPDGPGNFDQLMDTVDLIAANSYNTTGPGFLPFIPGGGLYASALGDFLATG
                     INRFVNMWGQAPVGAQIEHNVVRWLCDLFGYGDDARGILTSGGSMANLSALVAARKAR
                     LPEDFLSGTIYVSEQVHASNAKAAAIAGFPSANVRSVACTPELRMDVDALREMLAADR
                     AAGLTPFFVIASAGTTNTGAIDPIAAIADVVAGQDLWLHVDAAYGGFFRLTERGRAAF
                     AGIERADSITLDPHKGMFLPYGTGSLVVRDGARLREAHFVGAAYLQDFPPEDELPNFT
                     EYSPELSRDFRGLRVWFPLMLHGVDAFREALDEKLDLARYLYEELLTIPELELPWEPE
                     LTVVPFRLRDGSDVADRRLLDLINASKRVVLSSTVVEDRFTIRACILSHRTHRDRVEE
                     CAQIVRRAVADVTAR"
     gene            complement(3559..3762)
                     /locus_tag="VIH70_00960"
     CDS             complement(3559..3762)
                     /locus_tag="VIH70_00960"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY8114961.1"
                     /translation="MCRSIKRLREGDVPATETEIREAALQFVRKVSGYRAPSKRNEDA
                     FNGAVDEVADASRRLLEAVTHSA"
     gene            3853..5883
                     /locus_tag="VIH70_00965"
     CDS             3853..5883
                     /locus_tag="VIH70_00965"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY8114962.1"
                     /translation="MFDAMERLNPEQRAAVEHLGGPLLVVAGAGSGKTWTLACRVANL
                     VDRGIPPERILLLTFTRRASREMLSRAERLTGDRALGRVWGGTFHATANRLLRLHGGA
                     LGLSPGFTVLDRTDTADLMDLIRGEMGFGQGERRFPRKDTLADVYSRTVNGRTKLAEV
                     LEREYPWCAEEAESIRTIFERYTERKRAQSALDYDDLLLFWDALCRHPAAGPAISELF
                     DHILVDEYQDTNPLQADILASLRPDGDGLMVVGDDAQAIYAFRSATVANILEFPSRFP
                     GTTIVKLERNYRSTRPILEATNAVIALSPQRHEKTLWTERTGGPLPSLLTCIDERDQA
                     DAVCGSILDHRERGTPLREQAVLFRAGHHSDVLEVELTRRNIPFVKYGGLKFLESAHV
                     KDALAVLRIADNPRDEVSWFRVLQLLDHVGPAGARRVMAAIGVSAPSDPMTRFLQQAI
                     AVPAPARGELEGLREAFRDLAVEASPPPAQVERVRRFLEPLLRRRYDQPEPRLRDLEQ
                     LEQLASGFESRARFVTDLTLDPPVSTSDLAGPPHLDEDYVILSTIHSAKGGEWDVVHV
                     IHAADGMIPSDMSAGDPEQLEEERRLLYVALTRARDELHVLFPLRYHRSNRGYEDRHW
                     YAQLTRFLPQEVRSRFSLETTYVDASDERPGAPTPDATASGVDALLAGLWSD"
     gene            5904..6761
                     /locus_tag="VIH70_00970"
     CDS             5904..6761
                     /locus_tag="VIH70_00970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8114963.1"
                     /translation="MEVLRPGDAVEFLRLAGALLERDEARNQLPLGIAGNLLARPDAF
                     DVVRFWVVRDGEESVAAAVRTEPFNLVLGDPSAEAALGPLLEAVVVDDPEVPGIVGNV
                     PFVEPAAERLAGASGRSAECILFQGVYGLTVVRDVARAPGEPRPAGPTDRGLLLDWLR
                     AFVTESVPDPDEAFRRLERTLESRFGIASAGFWLWEDDREPVSLSGFSGPTPTGIRIG
                     PVYTPPEHRRRGYATTLVADQSAWLLEHGHRACFLYTDLANPTSNRIYVDIGYERVCD
                     AMEFVFRTA"
BASE COUNT         1000 a         2471 c         2378 g          936 t
ORIGIN      
        1 gcgagcggga ccgtgacgag gagcacggcg atcgtcctgg acgggaagat gatccaggcg
       61 atcgggatgt cctcggacag ccggcccatc gcgaacaggc ccagtccgaa gaacacggcc
      121 gcgccgatcg ccagcgtcac cgcgagtccc tctcgccgat cggaacccga gtcgtccgtt
      181 cgggacatgc tcgacaagac gacgccgacg acgatcgccg cgagggcgat gccggcgccg
      241 gtcgcgacct gctcgccggc gatcaccgcg atgacggcgg ccgcggctcc ctgtgccgac
      301 gtgatcggcg cgaccacccc caccttgccg acgcggagcg agctgtacac gagcaggagc
      361 ccggtcgtgt tgccgaccgc ggtgacgacg agcaacacca cctgctcccg cccgagcccg
      421 tcgggcacgc cctgcgcgag cgcgaacggc gccgagatca ccgagccgat cgccagcacc
      481 caccccagca ccgcgacggg cgggatcatc cgcgtcgacc ggctgatgca cagcgccgag
      541 gccgcccaca tcgcggccgc gaccaggccg ccgagtatcg cgatcaccgg gcgagcgcat
      601 cctcgagcgg tccacctttg tgctcgccgc gcgcgggaca cgtgatcacg gcggaaaggt
      661 tacgcgggca cccgtcgccg ggcgtggtgc cgggcgaccc gggtgcggtt cccgcaggcg
      721 ttcgagcacc acacgcggtc cgaacgcgtc gcctcgaagt agctcccgca ggcgccgcaa
      781 cgccgcgctg gccggtccgg gtcgcccagc agctcgatcg ccgaccatga gatgcgggcg
      841 aggagcaggt gggtcggacc ggccgacagc gaggtgtcga cggccccgtc gggcgccaga
      901 cgcctgacgc ggggcacgcg ggcgctcgcc tcgttgatgt gctcgacggc ggcggcgggg
      961 aaggtaccgc cgccgatcga ggcgtcgagc agctcgcgga tcgaggcccg cagggtcacg
     1021 aactccgcca gccggagcga gacctcggcg cggtcctcgg gttcgagcgc gccttccgcc
     1081 tcgagccagt ccgcgagatc gccggccgag cccagccgtg cgttcgcgag cgacaggacc
     1141 tcggtcctac tcatggtctg agtctgatct ctccattagc tcgatacaag cttgaaatgc
     1201 gttctacgca cccaacgcat tagtaggcga aaaagcttcg gaggcccgct acctgatcag
     1261 gcgcgagaga cgccggtcgg ccaggacctt gccgccggtc tggcacgtgg ggcagtaggt
     1321 gacctcgtgc gactcgtagg agacccgccg gaggtcgtcc ccgcacaccg gacagggctc
     1381 gccgtagcgt ccgtggaccg tgaaatgatc gccgatcttg gtctggaggc cgcccgtacg
     1441 gcgccgctcg gccgcgagcg cctcgtcgag cacgctcccg accgcggcga ggagcgcctt
     1501 ccgctgcccc ggttcgagcg acgcgaggga cgcgtacggc gagagcttcg cgcggtgcag
     1561 gatgtcgtcc gagtacccgc ggccgatccc cgccaccgtc cgctggtcgc gcaggatcgt
     1621 gtgcacgcgc cggccgtcct ccccttccaa gaccagccgc tcgaactcgg gcgagtcggg
     1681 ctcgggcccg agcttggcga gcgggccctc gtcgccctcg gcaagcaccc accagccggc
     1741 cttgcgctcg cgaccgaact cctttaccag caccgacggg cggtcctcga accggaaccg
     1801 caccaccgag ccccgtggct tcgtcgactt cggggggtcc tcgacgtcga cgcgtccgcc
     1861 ctgcgacagg tgcacgagga cacggggccc gccgagctcc aaaaccaggt acttcccgcg
     1921 gcgccccacc cgttcgagga tccggccggc gagctcggag gcgcgagggg tcacggtctt
     1981 gagggaggag aactgcacca ggtcggccgc cgcgaacgcc gagccgccga ccgcctccgt
     2041 cagccgttcg gcgagcgcct gcacctcggg gagctcgggc atcgtcagac cccgatcgac
     2101 gaacgcctcg agaccgcggt caacgcgccg tcacatcggc cacggcgcgg cggacgatct
     2161 gcgcgcactc ctcgacgcga tcgcgatgcg tccgatgcga caggatgcac gcccggatcg
     2221 tgaatcggtc ctcgacgacc gtgctcgaga gcacgacgcg cttggaggcg ttgatcaggt
     2281 cgagcagtcg gcggtcggcg acgtccgagc catcacggag ccggaagggc acgacggtga
     2341 gctcgggctc ccacggcagc tcgagctcgg ggatcgtcag gagctcctcg tacaggtacc
     2401 gggccaggtc gagcttctcg tcgagcgcct cgcggaacgc gtcgacgccg tggagcatga
     2461 gcgggaacca cacgcgcagg ccgcggaagt cgcgcgagag ctcgggcgag tactcggtga
     2521 agttcggcag ctcgtcctcg ggcgggaagt cctggaggta ggcggcgccg acgaagtggg
     2581 cctcgcgcag tcgcgcgccg tcgcgcacca cgagcgaccc cgtcccgtag ggcaggaaca
     2641 tccccttgtg cgggtccagg gtgatcgaat cggcccgctc gatcccggcg aaggccgccc
     2701 gaccgcgttc ggtgagccgg aagaagccgc cgtacgccgc gtcgacgtgg agccacaggt
     2761 cctgcccggc aacgacgtcg gcgatcgccg cgatcgggtc gatcgcaccg gtgttggtcg
     2821 tcccggccga cgcgatcacg aagaacggcg tcaggccggc cgctcgatcg gccgcgagca
     2881 tctcgcgcag cgcgtcgacg tccatccgga gctcgggcgt gcacgcgacc gagcggacgt
     2941 tcgcgctcgg gaacccggcg atcgccgccg ccttcgcgtt ggacgcgtgc acctgttcgg
     3001 agacgtagat ggtgcccgag aggaagtcct cggggagcct ggccttgcgc gccgcgacca
     3061 gcgccgacag gttcgccatc gagccgccgg acgtgaggat gcctcgggcg tcatcgccgt
     3121 acccgaacag gtcgcagagc cagcgcacga cgttgtgctc gatctgcgcc ccgaccgggg
     3181 cctgccccca catgttcacg aaccggttga tcccggtcgc gaggaagtcg ccgagcgccg
     3241 aggcgtagag cccgcctccc gggatgaacg ggaggaagcc cggtccggtg gtgttgtagg
     3301 agttggccgc gatcaggtcc acggtgtcca tcagctgatc gaagttcccg ggtccatccg
     3361 gcggggcgcc gcgcaccgag cgggcggcct cgatcgcgcc atcgacgtcc atcgccgccg
     3421 cctcgtcctg ggactggacg aactcgacca ggtacccgag cgcggcctcg cccatcgcgc
     3481 gcatctgctc ggggcccggt tcgaggggat acgacatcgc gcccatgcta cggaacgcgg
     3541 gcgcggacag ccgttcggct acgcggagtg ggtgaccgcc tcgagcaggc gtcgggaggc
     3601 gtcggccacc tcgtcgacgg ccccgttgaa cgcgtcctcg ttccgcttcg agggcgcccg
     3661 gtagccgctg accttgcgga cgaactgcag ggccgcctcc cggatctcgg tctcggtggc
     3721 cggaacgtcg ccctcgcgga gccgcttgat gcttctgcac atgccctcga tggtaccggc
     3781 tgccgcatcg agctccggac ccggccgagg tccttgcccg cgtccggccg ccatcctatc
     3841 ctgctcgaac gcatgttcga cgcgatggag cgcctcaacc cggagcagcg agcggccgtc
     3901 gagcacctgg ggggcccgct gctggtggtc gcgggggccg gctcgggcaa gacgtggacg
     3961 ctcgcctgcc gggtcgcgaa cctggtcgat cgcggcatcc cgcccgagcg gatcctgctg
     4021 ctgaccttca cccgccgcgc ctcccgagag atgctctcgc gtgccgagcg gctgaccggc
     4081 gaccgtgcgc tcggccgggt gtgggggggc acgttccacg cgaccgccaa ccggctgctg
     4141 cgcttgcacg gcggggcgct cggcctgtcg cccggcttca cggtccttga tcgaacggat
     4201 accgccgacc tgatggacct gatccgtggc gagatggggt tcggccaggg cgagcggcgg
     4261 ttcccgcgca aggacacgct cgccgacgtc tactcgcgca cggtgaacgg ccgcaccaag
     4321 ctcgccgagg tgctcgagcg cgagtacccg tggtgcgcgg aggaggctga gtccatccgc
     4381 acgatcttcg agcgctacac cgagcgcaag cgcgcgcaga gtgcgctgga ctacgacgac
     4441 ctgctcctgt tctgggacgc gctctgccgc cacccggccg ccgggcctgc gatctcggag
     4501 ctgttcgacc acatcctggt cgacgagtac caggacacga acccgctcca ggccgacatc
     4561 ctggcgtcgc tgcgtcccga cggcgacggc ctgatggtcg tgggcgacga cgcccaggcg
     4621 atctacgcgt ttcggagcgc gaccgtcgcc aacattctcg agttcccgtc gcggttcccc
     4681 gggacgacga tcgtgaagct cgaacgcaac taccggtcga cgcgcccgat cctcgaggcg
     4741 acgaacgcgg tgatcgcgct cagcccgcag cgtcacgaga agaccctgtg gaccgagcgc
     4801 acgggaggcc ccctcccctc cctgctcacc tgcatcgacg aacgcgacca ggcggacgcg
     4861 gtctgcgggt cgatcttgga ccaccgcgaa cgcggcaccc cgctgcgcga acaggccgtg
     4921 ctgttccgcg ccggccacca cagcgacgtg ctcgaggtcg agctcacacg gcgcaacatc
     4981 ccgttcgtga agtacggcgg gctcaagttc ctcgagtcgg cgcacgtcaa ggacgcgctc
     5041 gcggtgctcc ggatcgcgga caacccccgc gacgaggtgt cgtggttccg agtgctccag
     5101 ctgctcgacc acgtcggccc ggccggcgcc cgtcgggtga tggcggcgat cggcgtctcc
     5161 gcgccgagcg acccgatgac gcgcttcctc cagcaggcga tcgcggtgcc ggcgcccgcc
     5221 cggggggagc tcgagggcct ccgggaggcc ttccgcgacc tggcggtcga ggcctcgcct
     5281 ccccccgcgc aggtcgaacg cgtccgccgg ttcctcgagc cgctgctgcg ccggcgctac
     5341 gaccagcccg agccccgcct gcgcgacctc gagcagctcg agcagctcgc aagcgggttc
     5401 gagagccggg cgcgcttcgt gaccgacctc acactcgacc ccccggtctc gacctccgac
     5461 ctcgccgggc cgccgcacct ggacgaggac tacgtgatcc tgtcgacgat ccactcggcc
     5521 aagggcggcg agtgggacgt ggtgcacgtg atccacgcgg cggacggcat gatcccctcg
     5581 gacatgtcgg ccggcgaccc ggagcaactc gaggaggaac gccgcctgct gtacgtcgcc
     5641 ctgacccggg cgcgcgacga gctccacgtg ctgttcccgc tccgctacca ccgctccaac
     5701 cgcgggtacg aggaccggca ctggtacgcg cagctcacga ggttcctccc ccaggaggtc
     5761 cggagcaggt tctcgctcga gacgacctac gtggacgcga gcgacgagcg tccgggcgca
     5821 ccgacgcccg acgcgacggc gagcggcgtc gacgcgctgc tcgccggcct gtggtcggac
     5881 tgaccgcccg ctaacatcgg gcgatggagg tcctgcgccc cggcgatgcc gtggaattcc
     5941 tgcgcctcgc cggcgcgctg ctcgaacgcg acgaggcccg taaccagctc ccgctcggga
     6001 tcgccgggaa cctgctggcc cgcccggacg ccttcgacgt cgtccgcttc tgggtcgttc
     6061 gggacgggga ggaatccgtc gcggcggccg tccggaccga gccgttcaac ctcgtgctcg
     6121 gcgatccgtc ggcggaggcg gcgctcggtc ccctgctcga ggcggtggtg gtggacgacc
     6181 ccgaggtccc cggcatcgtt ggcaacgtgc cgttcgtcga gccggcggcc gagcgactcg
     6241 cgggtgcgag cggccggagc gccgaatgca tcctgttcca gggcgtgtac ggcctgacgg
     6301 tggtgcgcga cgtggcgcgg gcgccgggcg aaccgcgtcc ggcggggccg acagatcgag
     6361 ggctcctcct cgactggctg cgggcgttcg tcaccgagtc ggtccccgat cccgacgagg
     6421 ccttccggcg gttggaacgc accctcgaga gccgcttcgg catcgccagc gccgggttct
     6481 ggctctggga ggacgaccgg gagcccgtgt cgttgtccgg tttcagcggc cccacgccga
     6541 cggggatccg gatcggcccg gtctacacgc cgcccgagca tcgccggcgc gggtacgcga
     6601 cgaccctggt ggccgaccag agcgcctggc tgctcgagca tgggcatcgc gcgtgcttcc
     6661 tctacaccga cctcgcgaac ccgacgtcca accgcatcta cgtggacatc gggtacgagc
     6721 gcgtctgcga cgcgatggag ttcgtcttcc gaacggcctg aagctacgtc gcggaccgct
     6781 tcttc
//
LOCUS       DATBRK010000028         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_725103, whole genome shotgun sequence.
ACCESSION   DATBRK010000028 DATBRK010000000
VERSION     DATBRK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725103"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VIH70_00975"
     CDS             <1..665
                     /locus_tag="VIH70_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY8114964.1"
                     /translation="HAANFLHMLLGLEPSQEDAEVIDTTFVLYADHTMNASTFTARIV
                     ASTLSDMFSAITAAIAALKGPLHGGANEESMKMLEEVGSPGHAEAYVRDRLERHQKVM
                     GFGHAVYKTMDPRAVVLKRLSKETGDRHGDTSWYEISEAIERTVLEQRQLFPNVDFYA
                     ASVYHTLGIPTDLMTPIFAVARMAGWTAHVREQHADNRVIRPGSEYIGPRDQSWVPIE
                     ER"
     gene            complement(672..1106)
                     /locus_tag="VIH70_00980"
     CDS             complement(672..1106)
                     /locus_tag="VIH70_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HEY8114965.1"
                     /translation="MAETVTIQGQAYLKRNPLGVLGLTIITLGIYGLYWYYKVNEEIL
                     RYTGDQTISPSRSLLAVIPGFLLIVPPFIAYYNTSDHVVQMQQQRGIQSQISPALVVI
                     LALVIWIGMAAYVQEHLNRVWDSAATGGAQPAAPPAPPPVPA"
     gene            complement(1137..2063)
                     /locus_tag="VIH70_00985"
     CDS             complement(1137..2063)
                     /locus_tag="VIH70_00985"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY8114966.1"
                     /translation="MSEPGGRLEALVRSRHFCVTSEVVPPRSSDPEPVERQARELVGY
                     ADAVNVTDNPTASSHMSPVAGAAIVARTGLEPTLQLTVRDRNRLAIAADLLGAWGLGA
                     RNLLCLTGDPLSVGDEPDAVTVNDLGMLDLIALASTLREDSKLPSGTEIAPAPRYFVG
                     VADSPLAEHYDPARLEVKLDAGAGFVMTQIAYDLDRLAAWAEVVRVRGVFERSAVFVG
                     VAPLRSAKQARYMNEHLPGVHVPDALLRALEDAGPEAEALGTAQCVEIVSGLRAMPGI
                     SGVHVMGLGREESVRHVIEGAGLLPRPAPGPA"
     gene            complement(2060..>2232)
                     /locus_tag="VIH70_00990"
     CDS             complement(2060..>2232)
                     /locus_tag="VIH70_00990"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248009.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEY8114967.1"
                     /translation="VGVHRTEHGLVGDVAFDEVKEVAGMITPVPGGVGPMTTAMLLLN
                     TVSAAEKLSDQR"
BASE COUNT          364 a          772 c          773 g          323 t
ORIGIN      
        1 cgcacgcggc gaacttcctc cacatgctgc tgggcctcga gccgtcccag gaggacgccg
       61 aggtgatcga cacgacgttc gtgctgtacg ccgatcacac gatgaacgcc tccaccttca
      121 cggctcggat cgtcgcctcg acgctgtccg acatgttctc ggcgatcacc gcggcgatcg
      181 ccgccctcaa ggggccgctc cacggcggcg ccaacgagga gtcgatgaaa atgctcgagg
      241 aggtcgggtc gccgggccac gccgaggcgt acgtgcgcga ccggctcgag cgccaccaga
      301 aggtgatggg gttcgggcac gccgtctaca agacgatgga ccctcgggcg gtggtgctca
      361 agcgcctctc gaaggagacg ggcgatcggc acggggacac gtcgtggtac gagatctccg
      421 aggcgatcga acgcaccgtc ctcgagcaga ggcagctgtt cccgaacgtc gacttctacg
      481 ccgcgagcgt gtaccacacc ctcggcatcc cgaccgacct catgacgccg atcttcgcgg
      541 tcgcccggat ggccggatgg acggcccacg tgcgcgaaca gcacgcggac aaccgcgtga
      601 tccgccccgg atcggagtac atcggacccc gcgatcagtc gtgggtcccg atcgaggagc
      661 ggtaggccgg ctcaggccgg aaccggaggc ggcgccggcg gagccgcggg ctgcgcgcca
      721 cccgtcgcgg ccgagtccca gacgcggttc aggtgctcct gcacgtaggc cgccatcccg
      781 atccagatga cgagcgccag gatgacgacg agcgccggcg agatctgcga ttggatccct
      841 cgctgctgct gcatctggac gacgtggtcc gacgtgttgt agtaggcgat gaacggcggg
      901 acgatcagca ggaagccggg gatcacggcc aggagcgatc gagacgggct gatcgtctgg
      961 tcgccggtgt agcgcagaat ctcctcgttc accttgtagt accagtacag gccgtagatg
     1021 cccaacgtga tgatcgtgag ccccagaacg ccgagcgggt tccgcttcag atacgcctgt
     1081 ccctggatcg tcaccgtctc ggccacgtcg cctcccccgt ctgttgctcg gcgagtctac
     1141 gccgggcccg gggcggggcg tggaagcagc ccggctccct cgatcacgtg gcggacggac
     1201 tcctcgcggc caagccccat cacgtgcacg cccgagatgc ccggcatcgc ccggagaccc
     1261 gagacgatct cgacgcactg ggcggtcccg agcgcctccg cctcgggccc ggcatcctcg
     1321 agcgcgcgga gcagggcgtc gggaacatgg acgccgggca ggtgctcgtt catgtagcgg
     1381 gcctgcttcg cgctccgaag cggcgccacg ccgacgaaca cagccgaccg ctcgaacacg
     1441 cctcgcactc gaacgacctc ggcccacgcc gccagccggt ccaggtcgta ggcgatctgc
     1501 gtcatcacga atccggcgcc ggcgtcgagc ttgacctcga gccgggccgg gtcgtagtgc
     1561 tcggcgagag ggctgtccgc gacaccgacg aagtaacggg gcgccggggc gatctcggtc
     1621 cccgatggga gcttcgagtc ctcacggagc gtcgaggcga gcgcgatgag gtcgagcatt
     1681 ccgaggtcgt tgaccgtaac tgcatcgggc tcgtcgccca ccgacagcgg gtcgccggtg
     1741 aggcacagca ggttgcgggc gccgagcccc caggccccga ggagatcggc cgcgatcgcc
     1801 agccggttcc gatcgcggac cgtgagctgg agggtaggct cgagacccgt cctggcgacg
     1861 atcgcggcac ctgcgaccgg cgacatgtgc gaactcgccg tcgggttgtc ggtgacgttg
     1921 acggcatccg cgtagccgac gagctcgcgc gcctggcgtt cgaccggttc gggatcgctc
     1981 gacctcggcg ggacgacctc ggaggtcacg cagaaatggc gcgagcggac aagcgcctcg
     2041 agccggccgc ccggttcgct caacgctgat cggagagctt ctcggccgcc gagacggtgt
     2101 tgagtagcag catcgcggtg gtcatcgggc ccacgccgcc ggggaccggc gtgatcatcc
     2161 cggcgacctc cttcacctcg tcgaacgcca cgtcgccgac caggccgtgc tcggtccggt
     2221 gcacgcccac gt
//
LOCUS       DATBRK010000029         3942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1880467, whole genome shotgun sequence.
ACCESSION   DATBRK010000029 DATBRK010000000
VERSION     DATBRK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880467"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..2103)
                     /locus_tag="VIH70_00995"
     CDS             complement(130..2103)
                     /locus_tag="VIH70_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8114968.1"
                     /translation="MLRRLRGRPGSHAAVWILTAALFAGSGLLWLGVRGEEPPFAGGP
                     SLPLWVLAVSFGVAEVFVMHIRIARHAQTFSLAEIPLVFGLAFTAPGGLVLAQTVGVG
                     IALAVHRRQKPLRLTFNVAQRAFTTLLAVVFVAVCRSALPAGWPSLWVSLFGATLLAD
                     LVGAMLINVAIGLSDDKMKLLDQVIGLGTSLTVANTALGIVGVMVFLQQPAAVLLVVA
                     PAATTYLAGKAFTDLQRKHDDLLQLQRATGLAQRSLEREDMVPVLLHHTREMFNADIA
                     EMLLWPEGRDQTPVRCQVGPGDEEIAFEPAALDPTEGVWARVASERESVLLSRPIRNE
                     ALRRFFGDRQIRDAIVAPVSSDDQLLGILTVADRLDDFSTFDHDDLELFQTLTNHVAV
                     ALRNSLLHERLERALAHETETSRLKDEFVATVSHELRTPLTNVQGSVKTLLRRDVRFT
                     PAEQHEFLMAADRHTERLKRLIEDILFAARVESDEPPSRPTQEIGLAGLITRLVEDEA
                     YGDGRERIDVVISDTVPPVLGIEEDVYRIVRNLIDNALKYSPANQRVTVTVGTDADDV
                     LVRVHDRGPGIPPDQRDRIFDRFYQIDQSDTRRVGGVGMGLYICRRAAERLGARIWLD
                     RSDASGSVFAVRLPTVDRTGHEAADVVTVIARA"
     gene            complement(2104..3672)
                     /locus_tag="VIH70_01000"
     CDS             complement(2104..3672)
                     /locus_tag="VIH70_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8114969.1"
                     /translation="MGTRISIIDGFAAEVPIGELSGLDGSPAIVSVTPDAAVRLLGNV
                     DGIDPNRYPGSWLKVAKNSKLYEMWQRGWTGEGIDVALIDSGVAPVPGIDMQVVNGPD
                     LSFDSQAPNLTDIDTYGHGTHMAGLIAGRDAAIPPGKEDEEVDHYFVGAAPGARIVSI
                     KVAASDGATDVSQVIAAIDWIVQHRNSDGLNIRVLNLSFGTDGTQDYQLDPLAYAAEV
                     AWHHGIVVVVAAGNSGFGTAQLNNPAYDPYVIAVGGDDTRGTDDPKDDVIPSWQSRGN
                     TLRHPDLVAPGRSIVSLRDPGSFVDEANPGARVGASRFIKGSGSSQATAIVSGAIATI
                     LEQHPELTPDQVKALLMQTAVPLPDADPIAQGAGLINLHRAREAKISTVADAVQTWPR
                     STGLGSLQLARGSVVTQDGGTVLEGEQTIFGDTWDAPAWASSSWDGTSWSGGEWTART
                     WSGDCWCGSSWSARTWSARTWSGSSWSARTWSGRTWSARTWSGSGWSAGSWGADEAPS
                     ALSSGSWASTIWGD"
BASE COUNT          661 a         1320 c         1330 g          631 t
ORIGIN      
        1 tcgcgcggcc ccctttcgcg ttcccccgac agttccccta gggctcagga gccagcgaat
       61 cctggcgaac gcgaggaagg ctgcaagaag gacgagaccg gggatcggac ttcgggtagc
      121 agagagaagt caggcacgtg cgatgacggt cacgacgtcc gccgcctcgt gccctgtgcg
      181 atcgacggtc ggaaggcgaa cggcgaagac cgatccggag gcatcggacc ggtcgagcca
      241 gatgcgcgcc cccagtcgtt ccgcggcacg ccggcagata tagagtccca tcccaacacc
      301 gcccactcgc cgtgtgtcgg actgatcgat ctggtagaag cggtcgaaga tcctgtctcg
      361 ttggtcggga gggatccccg gtcctcgatc gtgcactcgg acgaggacat catcggcgtc
      421 ggtgccgacc gtgaccgtga ctcgctggtt cgcgggggag tacttcagcg cgttgtcgat
      481 caggttgcgc acgatgcgat agacgtcttc ctcgatacct aggaccggcg gcaccgtatc
      541 tgagatcacc acgtcgatac gctctcgccc gtcaccgtac gcctcatcct cgacgaggcg
      601 ggtgatcagg ccggcgagac cgatctcctg cgtcggcctc gagggcggct cgtcggattc
      661 gacacgcgcc gcgaacagga tgtcctcgat gaggcgcttg aggcgctccg tgtgtcggtc
      721 cgcagccatc aggaactcgt gctgctctgc gggcgtgaag cgaacatccc gacgcaggag
      781 cgtcttgacg gacccctgga cgttcgtgag cggggttcgg agctcatggg agaccgtcgc
      841 cacgaactcg tccttcagcc ggctcgtctc ggtctcgtgc gcgagcgcgc gctcgagccg
      901 ctcgtggagc aacgagttcc gcagggcgac ggcgacgtga ttggtgagcg tctggaacaa
      961 ctcgaggtcg tcgtgatcga acgtgctgaa gtcgtcgagt cggtcggcga ccgtcaggat
     1021 gccgagtagc tgatcgtccg acgacacggg cgcgacgatc gcgtcccgga tctgccggtc
     1081 tccgaagaag cgccggagcg cctcgttgcg gatcggccgc gagagaagca cggattcgcg
     1141 ctcagatgcc acgcgagccc agacgccctc cgtagggtcc agggccgcgg gttcgaaggc
     1201 gatctcctcg tcccccggcc cgacctgaca gcgcacgggg gtctgatcac ggccttccgg
     1261 ccacaggagc atctcggcga tgtcggcgtt gaacatctct cgggtgtgat gcaggagcac
     1321 cggaaccatg tcctcgcgtt cgagcgaccg ttgcgcgagc ccggtcgcgc gctggagctg
     1381 caggagatcg tcgtgcttcc tctggagatc ggtgaacgcc ttgcccgcga ggtaggtcgt
     1441 cgcagccggt gccacgacga ggaggaccgc cgccggctgc tggaggaaga ccatcacgcc
     1501 gacgatcccg agtgccgtgt tcgcgaccgt gagcgaggtg ccgagaccga tcacctggtc
     1561 gaggagcttc atcttgtcgt ccgacagccc gatcgcgacg ttgatcagca tcgcgcccac
     1621 gaggtcggcg aggagggtcg ctccgaagag ggacacccat agggacggcc accccgcggg
     1681 aagcgccgag cgacacaccg cgacgaagac gaccgcgagg agggtcgtga acgcacgctg
     1741 ggcgacgttg aaggtcagcc ggagcggttt ctgccgtcga tggaccgcca gcgcgatccc
     1801 gacacctacg gtctgggcga gcacgagtcc tcccggggcc gtgaacgcca gcccgaacac
     1861 gagagggatc tcggccagcg agaaggtctg cgcgtgccgc gcgatgcgga tgtgcatcac
     1921 gaacacttcc gcgactccga acgagacggc gagcacccac aacgggaggg acggtccgcc
     1981 ggcgaacggc ggctcctcgc ctcgtacccc caaccacagg aggccgctcc cggcgaagag
     2041 ggccgccgtc aggatccata ctgccgcgtg cgaaccggga cggccacgca agcggcggag
     2101 catctagtcc ccccagatgg tgctggccca ggaacccgag gagagggccg acggagcctc
     2161 gtccgcgccc cacgagcctg ccgaccaccc cgatccgctc cacgtgcgag cggaccaggt
     2221 ccttccgctc cacgtcctgg ccgaccagga cgagccgctc caggtccgtg cggaccacgt
     2281 acgcgcgctc cagctcgagc cacaccagca gtctcccgac cacgtcctgg cggtccactc
     2341 ccctccggac cagctcgtac cgtcccagct gctcgacgcc catgcggggg catcccaggt
     2401 atcaccgaag atcgtctgct ctccctcgag gaccgttccg ccgtcctggg tgacgacgga
     2461 gccccgggcc agctggaggg accctagccc cgtcgatcgg ggccacgtct gaacggcgtc
     2521 cgcaaccgtc gagatctttg cctcccgcgc gcgatggagg ttgatgagcc ccgcgccctg
     2581 ggcgatcggg tcggcgtcgg gcagtggcac cgcggtctgc atgagcagcg ccttcacctg
     2641 gtctggggtg agttcgggat gctgctccag gatcgtcgcg atggcccccg acacgatcgc
     2701 ggtcgcctgc gacgagccgc tgcccttgat gaatcggctg gctccgacgc gggcgcccgg
     2761 gttcgcctca tcgacgaagc tgccgggatc gcgaaggctg acgatcgacc tgccgggggc
     2821 gacgaggtcg ggatgccgga gcgtgttgcc gcgcgactgc caggacggga tgacatcgtc
     2881 cttcggatcg tccgttcccc tggtgtcgtc tccaccgacc gcgatcacgt acgggtcgta
     2941 cgcagggttg ttgagctgag cagtaccgaa gccggagttg cccgccgcga cgaccaccac
     3001 gatgccgtgg tgccacgcga cctcggccgc gtaagccagc ggatcgagct ggtagtcctg
     3061 cgtgccgtcg gtgccgaagg acaggttgag gacgcggatg ttcagtccat cgctgttgcg
     3121 gtgctgcacg atccaatcga tcgcggcgat gacctgcgat acgtcggtcg caccgtcact
     3181 cgccgccacc ttgatcgaga cgatgcgagc gccgggagcg gcacccacga agtagtgatc
     3241 gacttcctca tcctccttgc ccggcgggat ggcggcatcg cggccggcga tcagacccgc
     3301 catgtgggtg ccgtgtccat aggtgtcgat gtcggtcaga ttcggagcct gcgagtcgaa
     3361 cgagaggtcg gggccgttga ctacctgcat gtcgatgccc ggcacggggg cgacgcccga
     3421 gtcgatcagc gcgacgtcga tgccttcccc cgtccaaccc cgctgccaca tctcgtacag
     3481 cttcgagttc ttcgccacct tcaaccagga accgggatac ctgttcgggt cgatgccgtc
     3541 gacgttgccg agcaaccgaa ccgcggcatc cggggtgacg gagacgatcg caggcgatcc
     3601 atccaatccg ctgagctcgc cgatcgggac ctctgcggcg aacccgtcga tgatcgagat
     3661 gcgggtaccc acctttccgc cggcatcgag gacgaggcgt tccgcgacgt cgatggatcc
     3721 ccgctgtccc cgaacgatca ctgggacgag gtggtccgcc gctgcggtcg ccgctacggg
     3781 agggaggacc agggctgtga gcagaagcga aatcgcgagc gttcgacccc cgcgcccatc
     3841 tgttcgcgtc ccctgggtgt gcatgttcgc ctccgtcgtc agaggttcat cctgggggac
     3901 acgacgtagg tcgtgcatcc gacggatagt cgtttctgat gc
//
LOCUS       DATBRK010000030         6199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1735898, whole genome shotgun sequence.
ACCESSION   DATBRK010000030 DATBRK010000000
VERSION     DATBRK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735898"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="VIH70_01005"
     CDS             complement(<1..1775)
                     /locus_tag="VIH70_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEY8114970.1"
                     /translation="MVVRVPTATPAPGRRRLIIIVVAVVAAFLVLFTALSGFFVDVLW
                     FREVRLSDVFWTVLRTRVFLGLAFGLTFFVLLYSNLWIVRRITPRFHALTPEQEIIER
                     YRMQFEPYIRWLLPLFALVIAFFVALGVTSQWQTYLLWRNGSGLSFGNPEPLFGRDPA
                     FYVFSLPWLKFMQGWLFSALVGVTFLTALAHYLWGGIRPQAPGFVDKVTPQVKAHMSV
                     LLGLIMLTKAWGYYLGQFDLLTSTRGVVVGASYTDVNAQLPALRILVVIAIACAILFL
                     VNIRLRGWALPVIAVGLLALVSIIAGAAYPAFVQRFRVAPQEFQREQPFIVDNIEATR
                     RAFSLDGIQSSSRPIGESVTNEEVRANDATISNIRLWRPDVLRDNYISLQRFRSYYEF
                     NDVDVDRYVIDGQRRVLMISTREVSQAGIPEGGKTWQNVHLQYTHGFGSVASQVNTAT
                     SEGQPVFTLRDIPPVGAPALEGNGQRVYYGEGAPGDAAFVVVDTGTDELDYQGTATDD
                     QAQATFSYDGDGGIPVGGLFQRALFAWRFRDVNLLISDLIKGDSRIMIYRDITARVPK
                     AAPFLRFDLDPYSAVVDGRLVWIWDA"
     gene            complement(1850..2824)
                     /locus_tag="VIH70_01010"
     CDS             complement(1850..2824)
                     /locus_tag="VIH70_01010"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEY8114971.1"
                     /translation="MLRRNRVVLYVAIGVIAGLAFIVYLPWYAVGPGPARAVQPLIRF
                     EDRTRYESQGSFVMTSVRYTRLTGFDMLLAWLDPDRAVVARSVLYPDGESPQVQRERS
                     ISQMDSSKLDASAVVLRTLEGYPKDHGDGVLVESVVSGCAADGELFPGDRILSIEGEP
                     VDSYRDASRAIEAAPSGSTLRVDLSVDGEPETVDLVREPCGDSEDPLVGVRMINSFPF
                     DVSISSGEIGGPSAGLMWALGLYDLLTPGDLTDGRMIAGTGQIALDGTVIPIAGIDEK
                     LSAAADAGTSVFLVPRGNMKAARASGGHGLELVPVATFDDALAYLKGR"
     gene            2832..3206
                     /locus_tag="VIH70_01015"
     CDS             2832..3206
                     /locus_tag="VIH70_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114972.1"
                     /translation="MVRGDRANEGEADTLLAMPLVIVAVLSLLVGCAVYVGTVRTQGA
                     PPTTGFGETEPEETLEPFEPGAPAPGYAYLQVSTQGPGVRERLQGLVGVIVLLGVGAA
                     ALALALYELGHLINKTIEAFLD"
     gene            complement(3231..4238)
                     /locus_tag="VIH70_01020"
     CDS             complement(3231..4238)
                     /locus_tag="VIH70_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8114973.1"
                     /translation="MGRDLAIDLGTANTLVYRQGEGVVFNEPTVVAMNASNGKVEAMG
                     DEAWQMIGGRSGNVVALRPLRHGVMTEFDITQRMIEVVLRRVGVSRFPKPRVLSCVAS
                     ESSEVERRAVREAVKFAGGRDVVLVEEPLAAAIGARLPIHQPIGNLIVDIGGGTTEMA
                     VASMGGVVSGGSTPLGGFDLDAAIQDHLRREYDVAIGEKSAEAVKIAIGSAFPAPRGR
                     AAIVTGRALATGAPTEIRVTEDEIRQAMSEPVHQIVEAARRTIAESPPELTHDVLEAG
                     MFLTGGGGLLRGLDLLLSQECEVPVHLTDRPLETVVLGLGAMLEHLDDYRTSFQLIRK
                     R"
     gene            complement(4261..4686)
                     /locus_tag="VIH70_01025"
     CDS             complement(4261..4686)
                     /locus_tag="VIH70_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY8114974.1"
                     /translation="MRPDLLVRVTADPIGAGEAVEFVSDPAAGGTCVFLGTVRDHSDA
                     GAVTGLTYEAWEEQAVRRLEEVADELFERWPLRKVAFLHRFGELGLADVSVVVACSSA
                     HRAEAFEACRQGIEQLKRDVPIWKKEHLITGVSSWVMGS"
     gene            4775..5959
                     /locus_tag="VIH70_01030"
     CDS             4775..5959
                     /locus_tag="VIH70_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY8114975.1"
                     /translation="MSPTPEEQPFGVPPGDVWNDVPLFREIQRVLMSSSGPVNWELAR
                     QVGVASASWGTEDPAPSEEDRRGFDEAVRVAELQVAGFTGLEAPSDIPRVEAVRRGQW
                     VQANIEGLRGLLEPAAAKIGDAIAAAQREAVPEQAQAGVAQVLGQLSPLLLGAQVGTV
                     LGTLAQQVLGQYDIAVPRPDGAGALLFVVPNIARFEKEWSLDPTDFRTWIAIHEVTHR
                     FEFARPWALARFRELIDDFTSTLTLDVEELQQRLASLDASNPEGMQEMLAGQDSLFGA
                     VMDDEQRLKLRRVQAFMTASEGYGDHVMHALGAQMLPSYARIDEAMRRYRETEHVDPV
                     FERLLGIEVKREQYRLGRAFCDTVVELTDEATLARMWDSAEALPSMPELEEPRLWLAR
                     SA"
BASE COUNT          993 a         2145 c         2165 g          896 t
ORIGIN      
        1 gcgtcccaga tccagacgag ccgcccgtcc acgacggcgg agtacggatc gaggtcgaag
       61 cggaggaacg gtgcggcctt cgggaccctg gccgtgatgt ctcggtagat catgatccgg
      121 ctgtcgccct tgatcaggtc cgagatcaac aggttgacgt cgcggaagcg ccacgcgaac
      181 agcgcccgct ggaataggcc gccgaccggg atgccgccat cgccgtcgta gctgaacgtc
      241 gcctgcgcct ggtcgtcggt cgcggtgccc tggtagtcga gctcgtccgt gccggtgtcg
      301 accaccacga aggcggcgtc gcctggagcg ccctcgccgt agtagacgcg ctgcccgttg
      361 ccctcgagcg cgggcgcccc gaccggcggg atgtctcgca gtgtgaagac gggctgcccc
      421 tcggaggtcg ccgtgttcac ctgcgacgcc acggacccga acccgtgcgt gtactgcagg
      481 tggacgttct gccaggtctt ccctccctcc gggatcccgg cctggctcac ctcgcgcgtc
      541 gagatcatca ggacgcgtcg ctgcccgtcg atgacgtacc ggtcgacgtc gacgtcgttg
      601 aactcgtagt acgagcggaa gcgctgcaac gagatgtagt tgtcgcgcag gacgtccggg
      661 cgccacaggc ggatgttcga gatcgtcgcg tcgttcgcgc gcacctcctc gttcgtgacc
      721 gactccccga tcggccgcga gctgctctgg atcccgtcta acgagaacgc ccgtcgcgtc
      781 gcctcgatgt tgtccacgat gaacggctgt tcgcgctgga actcctgcgg cgcgacgcgg
      841 aaccgctgga cgaacgcggg gtacgcggcc ccggcgatga tcgagaccaa cgcgagcagc
      901 ccgaccgcga tgaccggcaa cgcccatccg cggagccgga tgttgaccag gaacaggatc
      961 gcgcaggcga tcgcgatcac caccaggatc cggagcgcgg gcagctgcgc gttcacgtcg
     1021 gtgtacgacg ctccgacgac gacccctcgg gtcgaggtga gcaggtcgaa ctgtccgagg
     1081 tagtaccccc acgccttcgt gagcatgatc agtccgagga gcaccgacat gtgcgccttg
     1141 acctgcggcg tgaccttgtc gacgaacccc ggggcctgcg gtcggatccc accccacagg
     1201 tagtgggcga gcgcggtcag gaaggtcacg ccgaccagcg ccgagaacag ccagccctgc
     1261 atgaacttca gccacggcag gctgaacacg tagaaggcgg gatcgcgacc gaacagcggc
     1321 tccggatttc cgaacgacaa cccggagccg ttgcgccaca gcaggtacgt ctgccactgg
     1381 ctcgtgaccc ccagcgcgac gaagaacgcg atcacgagcg cgaacagcgg aaggagccag
     1441 cggatgtacg gctcgaactg catccgatag cgctcgatga tctcctgctc gggggtcagc
     1501 gcgtggaagc gcggcgtgat gcggcgcacg atccacaggt tcgaatacag cagcacgaag
     1561 aacgtcagcc cgaacgcgag ccccaggaag accctggtcc gcaggaccgt ccagaacacg
     1621 tcggagaggc ggacctcgcg gaaccacagg acatcgacga agaaccccga gagcgcggtg
     1681 aacaggacga ggaaggcggc caccaccgcg acgacgatga tgatgagccg acgccgtccc
     1741 ggcgcgggtg ttgcggtcgg aacgcgtacg accataggta cctggtgtcc agccttcgcg
     1801 gtcgatggag gcgcaagcct atcaccggtc ctcgcggaga acgtgcctgc tagcgaccct
     1861 tcaggtacgc gagcgcgtcg tcgaaggtgg cgaccggaac gagctcgagc ccgtggccgc
     1921 ccgaggccct tgcggccttc atgttgcctc ggggaaccag gaagaccgag gtaccggcgt
     1981 cggcggccgc cgacagcttc tcgtcgatgc cggcgatcgg gatcaccgtg ccgtccaggg
     2041 cgatctgccc cgtcccggcg atcatccggc cgtccgtcag gtctccgggg gtgagcaggt
     2101 cgtacagacc gagcgcccac atcagcccgg ccgagggccc gccgatctcg ccgctcgaga
     2161 tgctcacgtc gaacgggaag ctgttgatca tccgcacccc gaccaacggg tcctcgctgt
     2221 cgccgcacgg ctcgcgcacg aggtcgacgg tctcgggctc gccgtcgacc gacagatcga
     2281 ccctgagcgt ggaccccgac ggggcggcct cgatcgcccg gctcgcgtcg cggtagctat
     2341 cgaccggctc cccctcgatc gacaggatcc ggtcacccgg gaagagctcg ccgtccgcag
     2401 cgcaccccga gacgacgctt tcgaccagca cgccgtcgcc gtggtccttc gggtagccct
     2461 cgagggttcg caggaccacc gccgacgcgt cgagcttgct cgagtccatc tgcgagatcg
     2521 agcgctcgcg ttggacctgg gggctctccc cgtccggata caaaacggaa cgagcgacca
     2581 cggcgcggtc ggggtcgagc caagccagga gcatgtcgaa accggtcagc cgcgtgtagc
     2641 gcacggaggt catcacgaag ctgccctgcg attcgtatcg tgtcctgtcc tcgaaccgga
     2701 tcagcggctg gaccgcgcgg gccgggcccg gaccgaccgc gtaccagggg aggtagacga
     2761 tgaaggcgag ccccgcgatc accccgatcg ccacgtagag aacgacacgg ttccggcgca
     2821 gcatgggagc gatggtacgg ggagatcgcg cgaacgaggg agaagcggat acgctcctcg
     2881 cgatgccgct cgtgatcgtg gccgtgctgt cgctgctcgt cgggtgcgcc gtgtacgtcg
     2941 ggacggtccg aactcaaggc gcgccgccga ccacggggtt cggggagacg gaaccggagg
     3001 agaccctcga gccgttcgaa ccgggagccc cagcccccgg ctacgcctac ctccaggtct
     3061 cgacgcaggg cccgggggtc cgcgaacggc tgcaggggtt ggtcggcgtc atcgtcttgc
     3121 tcggggtcgg cgccgcggcg ctcgcgttgg cgctctacga actcggccac ctgatcaaca
     3181 agacgatcga ggccttcctg gactagcggt cggtcctgct ccgtcgccgg tcagcgcttc
     3241 cggatgagct ggaacgacgt ccggtagtcg tcgaggtgtt cgagcatcgc ccccaggccg
     3301 aggaccacgg tctcgagcgg ccgatcggtg aggtgcacgg gtacctcgca ctcctgcgac
     3361 aggagcaggt ccagcccgcg caggagaccg ccgccgccgg tgaggaacat gcctgcctcg
     3421 agcacgtcgt gcgtgagctc ggggggcgac tcggcgatcg tcctcctcgc ggcctccacg
     3481 atctggtgga cgggctcgga catcgcttgg cggatctcgt cctcggtgac gcggatctcg
     3541 gtaggggcgc cggtcgcgag cgcccgaccc gtcacgatcg ccgcgcgtcc ccgcggggcg
     3601 gggaacgcag agccgatcgc gatcttgacc gcttcggccg acttctcccc gatcgcgacg
     3661 tcgtactcgc gacgcaggtg gtcctggatc gcggcatcga ggtcgaatcc gcccaggggc
     3721 gtcgagccgc cggagacgac gccgcccatc gaggccaccg ccatctcggt ggtgcctccg
     3781 ccgatgtcca cgatcaggtt cccgatcggc tggtggatcg ggagacgggc gccgatcgcg
     3841 gcggcgagcg gctcctcgac cagcacgacg tcgcgccctc cggcgaactt cacggcctcc
     3901 cgaacggccc ggcgctcgac ctcggaggat tcgctcgcga cgcaggacag cacgcggggt
     3961 ttcgggaagc gggagacgcc cacccggcgc aggaccacct cgatcatccg ctgggtgatg
     4021 tcgaactcgg tcatcacccc gtgacgcagc gggcggaggg ccacgacgtt gcccgaacgc
     4081 ccaccgatca tctgccaggc ctcatcgccc atggcctcga ccttgccgtt cgatgcgttc
     4141 atcgcgacga cggtcggctc gttgaacacg accccctcgc cctggcggta gacgagcgtg
     4201 ttcgcggtcc cgaggtcgat cgcgaggtcg cgccccatca gccgttcggg gtcccgaacg
     4261 tcacgacccc atcacccagc tcgagacgcc ggtgatgagg tgctccttct tccagatcgg
     4321 cacgtcccgc ttcagctgct cgatcccttg cctgcacgcc tcgaacgcct cggcgcggtg
     4381 cgccgacgag cacgccacca cgacggagac gtcggcgagc ccgagctcgc cgaaccggtg
     4441 caggaacgcg accttgcgaa gcggccagcg ttcgaacagc tcgtcggcga cctcctccag
     4501 ccggcgcacg gcctgctcct cccatgcctc gtacgtgagg cccgtcacgg caccggcatc
     4561 cgagtgatcc cgcaccgtcc ccaggaagac gcaggttccg cccgccgcgg ggtcgctcac
     4621 gaactcgacc gcctcgccgg cgccgatcgg atccgccgtg acgcgcacga gcaggtcggg
     4681 tcgcacgcac ccatcgtagc gagcggtccg ggccttcgag ggggcatggg gccgaggcgc
     4741 aggcgtaacg cgggcgcggc cgtaggatgg cggcatgagc ccaacgcccg aggagcaacc
     4801 gttcggggtc ccgcccggcg acgtctggaa cgacgtgccg ctgttccgag agatccagcg
     4861 ggtcctgatg tcgtcgagcg gaccggtgaa ctgggagctc gcgaggcagg tgggggtcgc
     4921 gtcggcgtcc tggggcaccg aggacccggc cccgagcgag gaggatcgtc gcgggttcga
     4981 cgaggccgtc cgggtcgccg agctccaggt ggccgggttc accggcctcg aggcgccctc
     5041 cgacatcccg agggtcgagg ccgttcggcg cgggcagtgg gtgcaggcga acatcgaggg
     5101 gctgcggggg ttgctcgaac cggccgccgc gaagatcggc gacgcgatcg cggcggcgca
     5161 gcgggaagcc gtgccggagc aggcgcaggc cggggtggcg caggtgctcg gccagctgtc
     5221 gccgctgctc ctcggggcgc aggtgggcac cgtgctcggg acgctcgccc agcaggtctt
     5281 gggtcaatac gacatcgccg tgccgaggcc cgacggcgcc ggcgcgctgc tgttcgtcgt
     5341 ccccaacatc gcccgcttcg agaaggagtg gagcctggac ccgaccgact tccggacctg
     5401 gatcgcgatc cacgaggtga cgcaccggtt cgagttcgcg cgtccgtggg ccctcgcgag
     5461 gttccgggag ctgatcgacg acttcacgtc gacgctcacg ctcgacgtcg aggagctcca
     5521 gcaacggctc gcctcgctcg acgcttcgaa cccggagggc atgcaggaga tgctcgccgg
     5581 acaggactcg ctgttcggcg ccgtgatgga cgacgagcag cggctcaagc tccgccgggt
     5641 ccaggcgttc atgaccgcat ccgagggcta cggcgaccac gtgatgcacg cgctcggggc
     5701 gcagatgctg ccgtcctatg ctcggatcga cgaggcgatg cggcggtacc gcgagacgga
     5761 acacgtcgat cccgtcttcg agcgattgct gggcatcgag gtcaagcgcg agcagtaccg
     5821 gctcggccgc gccttctgcg acacggtcgt ggagctcacc gacgaggcga ccctggcgcg
     5881 gatgtgggac tccgccgagg cgctcccgtc gatgcccgag ctcgaggagc cgcgtctgtg
     5941 gctcgcgcgc agcgcctgaa ccggcgtcgc gccgaagcgc gtgagcggac ggcacgggcg
     6001 cgatccgtga gcgggcgggc gtcgggtatc ctctcgcggc gatgaccgtc ggcgagctcg
     6061 tctccgccaa cgtcgtggac cagcgtgtct gggccacctc cggcgaacag ggtcccccgg
     6121 gcgtctacct gctcggctcg ccaggtacgg cgcttccctt cgtcgtcacc cgcgcgtgga
     6181 aggtcgggac gggcatggt
//
LOCUS       DATBRK010000031         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_291598, whole genome shotgun sequence.
ACCESSION   DATBRK010000031 DATBRK010000000
VERSION     DATBRK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291598"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VIH70_01035"
     CDS             complement(<1..465)
                     /locus_tag="VIH70_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114976.1"
                     /translation="MALPRRARRLWASWRAEQRTLRQGLVALALSTLAGFVAGLTLAH
                     ITGTLQELPGLIVLIPAAVGMKGTIFGAIGARLGTANVAGLLEPTLRPGSVLQRNVYV
                     AVVTTFSSALWLAVLSVLASAAFGEPSISLWRLATVSIVGGAIGSALILMITL"
     gene            complement(596..1516)
                     /locus_tag="VIH70_01040"
     CDS             complement(596..1516)
                     /locus_tag="VIH70_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HEY8114977.1"
                     /translation="MTSFDLRLRGPGGEPVDLVRTLNSHGFVDLPPMRPSADYRSVEL
                     TVRAGRGRPRTVRIEAGRGGRARVSVLGAPASERATDRLAATVRHVLRLDADLSAFYE
                     AASGDPDLAWVTSGAGRMVQSPTVFEDVVKTICTTNCTWSATKRMVHAIVEHLGERAP
                     GAEPHGPWGRAFPTPAAMAEAGESFYTDVARTGYRGAYLMRLARSVDDGELDLEAFAT
                     ATADELSDEELEERLLALPGVGPYAAAHIMMTLGRYHRLILDSWTRPTYANKVGRAKA
                     TDAGIERRFRRYGPYAGLAFWLFLTRDWVD"
     gene            complement(1513..>2085)
                     /locus_tag="VIH70_01045"
     CDS             complement(1513..>2085)
                     /locus_tag="VIH70_01045"
                     /inference="COORDINATES: protein motif:HMM:NF023134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEY8114978.1"
                     /translation="FDFSPHGDTPPAPGDLELLQRFLNLHVHDAEGTHLEPPAEQIRG
                     FLVERNLLSEHERFTDADRETYLGLRDAIRNLILAGDDEPVSARDADVLDRIGLASGL
                     HPHFHAAHPPTLEPRGTGVAAAYGAVVAIAFVSAFDGSFDHLKLCADETCRSVFYDRS
                     KNRSGRWCSMSTCGNRAKVRAWRERQRADG"
BASE COUNT          310 a          739 c          755 g          281 t
ORIGIN      
        1 cagcgtgatc atcaggatca gcgccgaccc gatcgcgccg ccgacgatcg agaccgtcgc
       61 gaggcgccac agcgagatcg acggctcgcc gaacgccgcc gaggcgagca ccgacaggac
      121 cgcgagccac agggccgacg agaacgtcgt cacgaccgcg acgtagacgt tccgctgcag
      181 gacgctgccg ggccggagcg tgggctcgag cagaccggcg acgttcgcgg tgccgagccg
      241 agcgccgatc gccccgaaga tcgtcccctt catcccgacc gcggccggga tcagcacgat
      301 caggccgggc agctcctgga gcgtcccggt gatgtgggcc agggtgagcc cggcgacgaa
      361 ccccgccagc gtgctgagag cgagcgcgac cagaccctgt cgcagggtcc gttgctctgc
      421 gcgccaagag gcccacagac gccgcgcacg acgtggaagc gccataacgg ttcgctcatc
      481 ggatccttcg cattccttgg ctcactcagg cggccggggg gctccgcgcg cccgaggcag
      541 tatgcgctcg gcgtccctgc gggacaaccc ttcgcgcgtt cgatggacgg gtgtttcagt
      601 cgacccagtc gcgcgtcagg aacagccaga aggcgaggcc cgcgtacggt ccgtagcggc
      661 ggaaccggcg ctcgatgccg gcgtcggtcg ccttcgcgcg tccgaccttg ttcgcgtagg
      721 tgggccgggt ccacgagtcc aggatcagcc ggtggtaccg accgagggtc atcatgatgt
      781 gcgcggccgc gtagggcccg acccccggca gcgcgagcag ccgttcctcg agctcctcgt
      841 ccgacaactc gtccgccgtc gcggtcgcga acgcctcgag gtcgagctcc ccgtcgtcga
      901 ccgaacgcgc gagccgcatc aggtacgcgc cgcggtaccc ggtgcgcgcc acgtccgtgt
      961 agaaggactc cccggcctcg gccatcgccg ccggcgtggg gaaggctcgg ccccatgggc
     1021 cgtgcggctc cgcgccgggc gcccgttccc ccagatgctc gacgatcgcg tggaccatcc
     1081 gcttggtggc ggaccacgtg cagttggtgg tgcagatcgt cttgacgacg tcctcgaaca
     1141 ccgtcgggct ctgaaccatg cgccccgccc cgctcgtcac ccacgcgaga tccgggtcgc
     1201 cggacgcggc ctcgtagaac gccgagaggt cggcgtcgag gcgcaggacg tgacggaccg
     1261 tggcggcgag ccggtccgtc gcccgctccg acgccggggc tccgaggacc gagaccctcg
     1321 cgcgcccccc gcgaccggcc tcgatccgca cggtccgcgg gcggccgcga ccggctcgga
     1381 ccgtgagctc gaccgaccgg tagtcggccg agggccgcat cgggggcagg tcgacgaacc
     1441 cgtgagagtt gagcgtccgc acgaggtcga cgggctcgcc gcctggaccg cgcagacgca
     1501 ggtcgaacga ggtcacccgt ctgcccgctg gcgctcccgc caggcgcgaa ccttcgcccg
     1561 gttgccgcac gtcgacatcg agcaccaccg tcccgagcgg ttcttcgaac ggtcgtagaa
     1621 gactgaacgg caggtctcgt cggcgcagag cttgaggtgg tcgaagctcc cgtcgaacgc
     1681 gctcacgaac gcgatcgcga cgaccgcgcc gtacgcagcc gccacgcccg tgcccctcgg
     1741 ctccagggtg ggggggtgcg cggcatggaa gtgcgggtgc aggccggagg caagcccgat
     1801 ccggtcgagc acgtcggcgt cgcgcgccga gaccggctcg tcgtcgccgg ccaggatcag
     1861 gttccggatc gcatctcgga gcccgagata ggtctcacgg tcggcgtcgg tgaagcgttc
     1921 gtgttccgag agcaggttcc gctcgacgag gaagccgcgg atctgctcgg cggggggttc
     1981 gaggtgcgtc ccctcggcgt cgtggacgtg caggttgagg aaccgctgga ggagctcgag
     2041 gtctccgggt gccggggggg tgtccccgtg cggcgagaag tcgaa
//
LOCUS       DATBRK010000032         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1880641, whole genome shotgun sequence.
ACCESSION   DATBRK010000032 DATBRK010000000
VERSION     DATBRK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880641"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VIH70_01050"
     CDS             complement(<1..508)
                     /locus_tag="VIH70_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8114979.1"
                     /translation="MTAAVSTTDALELVGLERRFGEVVALDDVSFRVAAGEMVGFVGP
                     NGAGKTTAMRIVLGVLQADAGRVRWRGRDVDADTRRRFGYLPEERGLYPKMRVLDQLV
                     YLARLHGLERGDARSQADRMIELLGVADRARDRAESLSLGNQQRVQLAAALVHRPEVL
                     VLDEPFSGL"
     gene            complement(505..1437)
                     /locus_tag="VIH70_01055"
     CDS             complement(505..1437)
                     /locus_tag="VIH70_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8114980.1"
                     /translation="MRALVAAPSAPGGIELRDVDEPVPANGVAVVEVRACSLNRGECS
                     ELLIAEDGWRPGWDVAGVIAGPALDGSSPPAGTRVIAWANGGGWAERVAVRSGHLAPT
                     PDGLSFETASTLPVAGLTALGTLAFGGSLDGSSVAITGAAGGVGRFAVQLAHDAGAKV
                     TAVVGTPERGEGLRELGADDVVVGLEAKGEPFDLILESAGGPSLAAALVRISPDGTVV
                     SIGNSSKEPTTFDARGFYRRGRPTLRGYFATYELLEGGLGTGELARLVELVSSGQLRV
                     EVGLVVPWIDAASAVMALLERRVRGKAVLTVGSS"
     gene            complement(1437..>1867)
                     /locus_tag="VIH70_01060"
     CDS             complement(1437..>1867)
                     /locus_tag="VIH70_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY8114981.1"
                     /translation="ASVLLLPLVRFLPADDPRIRSTVHAIADELTEDGLVLRYRTEET
                     DDGLAGNEGTFTICSFWLVSAFVEIGELSRARDLCEKLLSYASPLELYAEEIDPKSGR
                     HLGNFPQAFTHLALINAVVHLIQAEMQLQRGFSMAHQIVA"
BASE COUNT          275 a          707 c          653 g          232 t
ORIGIN      
        1 ccaggcccga gaacggctcg tccagcacca gcacctccgg ccggtgcacg agcgcggccg
       61 cgagctggac gcgctgctgg ttgccgagcg agagcgactc ggcccggtcg cgggctcggt
      121 cggcgacgcc gagcagctcg atcatccgat cggcctggga gcgcgcgtcc ccgcgttcga
      181 ggccgtggag gcgcgccagg tagacgagct gatcgagcac gcgcatcttc gggtagaggc
      241 cgcgctcctc gggcaggtag ccgaaccggc gtcgcgtgtc ggcgtccacg tcacgtccgc
      301 gccagcgcac ccggcccgcg tcggcctgca gcacgccgag cacgatccgc atcgccgtcg
      361 tcttgccggc cccgttcggg ccgacgaacc cgaccatctc gccggccgcg acgcggaagg
      421 acacgtcgtc gagcgcgacg acctcgccga agcgccgctc caacccgacg agctcgagcg
      481 cgtccgtggt cgacaccgcc gcggtcacga cgacccgacc gtgaggacgg ccttgccgcg
      541 cacccgccgt tcgagcaacg ccatcacggc cgaggcggcg tcgatccacg ggaccaccag
      601 gccgacctcg acccgcagtt gcccgctcga gaccagctcg acgagcctcg cgagctctcc
      661 cgtgccgagc ccgccctcga gcagctcgta cgtcgcgaag taccccctga gcgtcggccg
      721 gccgcgacgg tagaacccgc gcgcgtcgaa cgtcgtgggc tccttcgagg agttcccgat
      781 cgacacgacg gtcccgtccg gcgatatgcg gacgagcgct gccgccaggc tcgggccgcc
      841 cgccgactcg aggatcaggt cgaagggctc cccctttgcc tcgaggccga cgaccacgtc
      901 gtcggccccg agctcgcgga gaccctcgcc gcgttcgggg gtgccgacca cggccgtcac
      961 cttcgcgccg gcatcgtgcg cgagctggac ggcgaaccgg ccgacgccac ccgccgcccc
     1021 cgtgatcgcg acgctcgacc cgtcgagcga cccaccgaac gcgagcgtgc cgagcgcggt
     1081 gaggcccgcg accgggagcg tcgacgccgt ctcgaacgag aggccgtccg gggtcggcgc
     1141 gagatggccg gagcgcacgg ccacgcgttc ggcccatccg ccgccgttcg cccaggcgat
     1201 gacgcgggtc ccggcgggcg ggctcgagcc gtcgagcgcc gggccggcga tcacgccagc
     1261 gacgtcccag ccgggtcgcc acccgtcctc ggcgatcagc agctccgagc attcgcctcg
     1321 gttcagcgag caggcccgga cctccaccac ggcgacgccg ttcgcgggga ccggttcgtc
     1381 gacgtcgcga agctcgatgc cgccgggcgc gctcggggcg gcgaccagcg cccgcatcag
     1441 gcgacgattt gatgcgccat cgagaagcct cgctgcaact gcatctccgc ctggatcagg
     1501 tggaccacgg cgttgatgag cgccaggtgc gtgaacgcct gcgggaagtt gccgagatgg
     1561 cggccgctct tcgggtcgat ctcctccgca tacagctcga gcgggctcgc gtacgagagc
     1621 agcttctcgc acaggtcgcg tgcacgcgag agctcgccga tctcgacgaa cgcggacacg
     1681 agccagaacg agcagatcgt gaacgtcccc tcgttgccgg cgaggccgtc gtccgtctcc
     1741 tccgttcggt agcggagcac cagcccgtcc tcggtcagct cgtccgcgat cgcgtggacc
     1801 gtgctgcgga tgcgcggatc gtccgcgggc aggaagcgga cgagcggcag gaggagcacg
     1861 gaggcat
//
LOCUS       DATBRK010000033         5757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1303170, whole genome shotgun sequence.
ACCESSION   DATBRK010000033 DATBRK010000000
VERSION     DATBRK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303170"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VIH70_01065"
     CDS             complement(<1..789)
                     /locus_tag="VIH70_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114982.1"
                     /translation="MSEPDLPIPLGPCSNGEYDPVPPSPVAREARRRALEACADGASR
                     LGVSRRTFLRSLCAASTVLLALDACTREAHRAAPEVPTTEPGGGFDVPPEATTEPEAA
                     EGAVGGEKFIMDVQGHLLEYDLNPVFHGEDFYAAFPQQYCGEDDPPVCYSIEHFLEAF
                     FIRSDTNVVVLSALPIYPQGSPLSPEIMAETRRIVEGLCRDERVLLHGQVLPNIGSLD
                     SVLRGMEDTASRYPIRAWKTFTHFPDFFDPGGGAWWLDDHERGLP"
     gene            864..2696
                     /locus_tag="VIH70_01070"
     CDS             864..2696
                     /locus_tag="VIH70_01070"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HEY8114983.1"
                     /translation="MDVRSFLADHPPFDALGPDGLDRVVASVQIEHFAPGTVILRQAG
                     APAKHLFVVRRGEAEILDDGRVIDEIGEGEVFGMWSLLGQVAPTATVRASEDTLCYLI
                     DPDVANEVLRSGAGVAFVAASVRRRIARVDETLKAEIDLVRYRQVGELVRRPPVTCDP
                     ATPIADAAELMARERVSSLLISNADGGVGILTDRDLRARVVAERRSGDTPVRDVMTPR
                     AQTVTATTMVGEVLLRMLEGGFHHFPIAEPDGTVVGVVTDTDLMGIGRDTPFALKSTI
                     ERAADREAVALAIGELPRVVSALVSSSADPVDVGHVIAFSIDAATRRLLELGMAEHGE
                     PAVPWAWLALGSAARQEQAIRTDQDHALAFDGDRDAADASLAPLAEFVTVGLEAAGIP
                     RCRGDVMATNEALRLTVQEWVERFDHWMRIQSPKASEQLSIVFDFRRVAGPLDADVTL
                     DAAMARAGEHPLFLQHLARRALDLRPPIGFVRGIIVDQHGEHPGKVDVKHGGIVIVGN
                     LARAYAIRAGLTAKRTLDRLRAAEGAGAIDAETREGLEEAFRFLWEVRLRHHVEQLGA
                     GEHPDDFVDPKELGTVARQGLKETFRIIARAQKALALDAGVALR"
     gene            complement(2693..3499)
                     /gene="fabI"
                     /locus_tag="VIH70_01075"
     CDS             complement(2693..3499)
                     /gene="fabI"
                     /locus_tag="VIH70_01075"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY8114984.1"
                     /translation="MLLEGKKLLVTGVLTRESIAYAAARALQEQGAEIVLTSFGRAMG
                     LTEKSARRLPDPVDVLEMDANDPAHVESVSAEIGSRWGRLDGFLHAIAFAPQDALGGN
                     FLHTPWESVATAIQTSAYSLKAIAAGMLPRMREHGGAIVSLDFDARIAWPIYDWMGVA
                     KASLESITRYLARDLGPHGIRVNTLSAGPLRTIAGKGIPGFQALAEGWGDRAPLGWDP
                     ADATPVADTIVFLLSNLARGITGEMVHVDGGYHAIGAQLPAPGNDEGGTG"
     gene            3540..4550
                     /gene="add"
                     /locus_tag="VIH70_01080"
     CDS             3540..4550
                     /gene="add"
                     /locus_tag="VIH70_01080"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY8114985.1"
                     /translation="MDLKAVAKVDLHRHLEGAIRLSTMLDLYRGSGHPLPQATEAELS
                     EQAQVLRPMGSLEEVLSRFALAQGAFVDEAAAERIAFEAVEDLAADNVRLAELRFSPE
                     FLCEPHGLDWDGAMAAIVRGVERAGRAFDVTLGLIAIASRNYGIGSAERTAAFALRHR
                     DHLVAFDLAGDEQAYPPSLYVDVVAELAGSGLELTTHYGESGGPAFPREAVEVLGSKR
                     LGHGVSVVADPEVTALVRERGVTLEMCPTSNRRTHAVADLADHPARRLLDQDVSVTIN
                     TDNPGLFAIDLSGELEVCVDVLGFSDDDLRRVTANALEASFVGKSAKADVRERHFGWV
                     DA"
     gene            complement(4553..5515)
                     /locus_tag="VIH70_01085"
     CDS             complement(4553..5515)
                     /locus_tag="VIH70_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114986.1"
                     /translation="MRSRVARAYALLCLAGLAACIGGSPEIVGSAGGRHGSPDRSTNR
                     WLEPANGTRGSASATKPYEGLGTWIDIYDTDVWEHPVHAVRAMAAHGIRTIYLQSSNY
                     HRHRPFVYPEGVRAIVDAAHRRRLEVVAWYLPGFRDLRLDLHRAVRTIRFETRSGNGF
                     DSFALDIESAEVRRPSLRTARLLRMSAAIRRAAGTEYPLGAIVTSPDRMVRTDPHFWP
                     GFPWRQLAETYDVFLPMTYYTYRVEGPRAAAWYTSQSVQIIRRQTSGLRVPIHVVGGI
                     SFDATGPETLGFVQTVLERDVIGASYYTFPGITLDQWKALSALR"
BASE COUNT          833 a         2006 c         2094 g          824 t
ORIGIN      
        1 cggtagtcca cgttcgtgat cgtcgagcca ccaggcgccg ccgccgggat cgaagaagtc
       61 ggggaagtgc gtgaacgtct tccaggcgcg gatcgggtag cggctcgcgg tgtcctccat
      121 cccgcggagc acggaatcga gcgatccgat gttcggcaag acctgcccgt gcagcagcac
      181 gcgctcgtcg cggcacaggc cctccacgat gcggcgggtc tcggccatga tctcgggcga
      241 cagcggcgat ccctgcgggt agatcggcaa cgcggagagc acgacgacgt tcgtatcgct
      301 gcgaatgaag aacgcctcga ggaagtgctc gatcgagtag cacaccggcg ggtcgtcctc
      361 gccgcagtac tgctgcggga acgccgcgta gaagtcctcg ccgtggaaga ccgggttgag
      421 gtcgtactcg agcaggtgac cctgcacgtc catgatgaac ttctcgccgc ccacggcgcc
      481 ctcggccgcc tcgggttcgg tggtggcctc gggcgggacg tcgaacccgc cccccggttc
      541 ggtcgtcggc acctccggcg cggcgcggtg cgcttcccgg gtgcaggcgt cgagcgccag
      601 cagcacggtc gacgccgcgc acagcgagcg caggaacgtc cggcgcgaga ccccgaggcg
      661 ggacgcgccg tccgcgcatg cctccagggc gcggcgacga gcctcgcgcg cgaccggcga
      721 gggcgggacc ggatcgtact cgccgttcga gcacgggccg agcgggatcg gcaggtcggg
      781 ttcgctcatg gctcctcgct gtggcagaca cgcccgaggg cgcgccggtt ccggcgagag
      841 ccggcccggc gtaggctccg gccgtggacg tccgttcgtt cctggccgac cacccgccgt
      901 tcgacgcgct cggccccgac gggctcgatc gggtcgtcgc gagcgtgcag atcgagcact
      961 tcgcccccgg caccgtgatc ctcaggcagg ccggcgcgcc ggcgaaacac ctgttcgtcg
     1021 tccgccgggg cgaggccgag atcctggacg acggccgggt gatcgacgag atcggcgagg
     1081 gcgaagtctt cgggatgtgg tcgctgctcg gccaggtggc acccaccgca accgttcggg
     1141 cgtccgagga cacgctgtgc tacctgatcg acccggacgt ggcgaacgag gtcctgcgtt
     1201 cgggcgccgg cgtggccttc gtggccgcga gcgtccggcg aaggatcgct cgggtcgacg
     1261 agaccctcaa ggccgagatc gacctcgtcc gctaccggca ggtcggcgag ctcgtccgcc
     1321 ggccacccgt cacctgcgac ccggcgacgc cgatcgccga cgcggccgag ctcatggcgc
     1381 gcgaacgcgt ctcgagcctg ctgatctcga atgccgacgg cggcgtcggc atcctgaccg
     1441 accgcgacct gcgggcgcgg gtcgtcgccg aacgccggag cggcgacacg ccggtccggg
     1501 acgtgatgac cccgcgtgcc cagaccgtga cggcgaccac gatggtcggc gaggtgctgc
     1561 tgcggatgct cgagggcggt ttccaccact tcccgatcgc cgagcccgac ggcaccgtcg
     1621 tcggcgtggt gacggacacc gacctgatgg ggatcggccg cgacacgccg ttcgcgctca
     1681 agagcacgat cgagcgcgcg gccgatcgcg aggcggtcgc gctcgcgatc ggcgagcttc
     1741 cgcgcgtggt ctcggcgctg gtgagctcga gcgccgatcc cgtcgacgtg ggccacgtga
     1801 tcgcgttctc catcgacgcc gcgacgcgcc ggctgctcga gctcgggatg gccgagcacg
     1861 gggagccggc ggtcccgtgg gcatggctcg ccctggggag cgccgcccgt caggagcagg
     1921 cgatccggac cgaccaggac cacgcgctcg cgttcgacgg cgaccgcgac gccgcggacg
     1981 cctcgctggc gccgctcgcg gagttcgtga ccgtgggtct ggaggccgcc gggatccccc
     2041 ggtgccgagg cgacgtgatg gccacgaacg aggcgctccg gctcaccgtg caggagtggg
     2101 tcgaacggtt cgatcactgg atgcggatcc agagccccaa ggcgagcgag cagctttcga
     2161 tcgtcttcga ttttcgaagg gtggcaggcc cgctggacgc ggacgtcacg ctcgacgccg
     2221 cgatggccag ggcaggcgag catccgttgt tcctccagca cctcgcccgg cgcgcgctcg
     2281 acctgcgacc gccgatcggt ttcgtgcgcg ggatcatcgt cgaccagcac ggggagcacc
     2341 ccgggaaggt ggacgtcaag cacgggggga tcgtgatcgt gggcaacctc gcgcgcgcgt
     2401 acgcgatccg cgccggactc acggcgaagc ggacgctcga tcgcctccgc gcggccgagg
     2461 gcgcgggcgc gatcgatgcc gagacccgcg aggggctcga ggaggccttc cggttcctct
     2521 gggaggtgcg gctccgccac cacgtcgagc agctcggcgc cggggaacac ccggacgatt
     2581 tcgtcgatcc gaaggagctc ggcacggtcg cgcgtcaggg tttgaaggag acgttccgca
     2641 tcatcgctcg tgcgcagaag gcgctcgcgc tcgacgcagg cgtcgcgctc cgctagccag
     2701 ttccaccctc gtcgttcccc ggcgccggga gttgcgcgcc gatcgcgtga tagccgccgt
     2761 ccacgtggac catctcgccg gtgatccccc gcgccaggtt cgaaagcagg aacacgatcg
     2821 tgtcggcgac cggcgtcgcg tcggcggggt cccacccgag cggcgcccgg tcgccccagc
     2881 cttcggcgag cgcctggaac ccggggatcc ccttgccggc gatcgtccgc agggggccgg
     2941 cggacagggt gttgacgcgg atcccgtgag gtccgaggtc gcgagcgagg taccgcgtga
     3001 tcgactcgag cgacgccttg gccacgccca tccagtcgta gatcggccac gcgatccggg
     3061 cgtcgaaatc gagcgagacg atcgcgcccc cgtgctcccg catgcgaggg agcatccccg
     3121 ccgcgatggc cttcagcgag taggcgctcg tctggatcgc ggtcgcgacg ctctcccagg
     3181 gggtgtgcag gaagttcccc ccgagcgcgt cctgcggcgc gaaggcgatc gcgtgcagga
     3241 agccgtcgag ccgtccccac cgggacccga tctcggccga gaccgactcg acgtgcgcgg
     3301 ggtcgttcgc gtccatctcc aggacgtcga ccgggtccgg gagccgtcgc gccgacttct
     3361 ccgtcagccc catcgcacgc ccgaacgagg tcaggacgat ctcggccccc tgctcctgca
     3421 gggccctcgc ggccgcgtac gcgatcgact cgcgcgtcag cacgccggtc acgagcagct
     3481 tcttgccttc gagcagcatc gggatcctct cggtcgcctc gcatgctatt gaaagggccg
     3541 tggatctcaa agccgtcgcc aaggtcgacc tgcatcggca tctcgagggc gcgatccgct
     3601 tgtcgacgat gctcgacctg taccggggat cgggtcatcc gctgccgcag gcgacggagg
     3661 ccgagctctc cgagcaggcg caggtgttgc ggcccatggg ttcgctcgag gaggtgctgt
     3721 cccggttcgc cctcgcgcag ggcgcgttcg tcgacgaggc cgcggcggag cggatcgcgt
     3781 tcgaggcggt cgaggacctg gcagcggaca acgttcggct cgccgagctg cggttctcgc
     3841 ccgagttcct gtgcgagccg cacgggttgg actgggacgg cgcgatggcc gcgatcgtcc
     3901 gcggggtgga acgcgccggc cgcgccttcg atgtcacgct ggggctcatt gcgatcgcga
     3961 gccggaacta cgggatcggg tcggcggagc gaacggctgc gttcgcgctc cgtcaccgcg
     4021 accacctggt ggcgttcgac ctggccggcg acgagcaggc ctatcccccg tcgctgtacg
     4081 tcgacgtcgt ggccgagctc gccggctcgg ggctcgagct cacgacgcac tacggtgagt
     4141 ccggcgggcc ggcgttccca cgcgaggcgg tcgaggtgct cggctcgaag cgcctggggc
     4201 acggggtgag cgtggtggcc gaccccgagg tgacggcgct cgtccgcgaa cgcggggtga
     4261 cgctggagat gtgcccgacc tcgaaccgcc ggacgcacgc cgtggccgat ctcgccgacc
     4321 atccggctcg ccggctgctc gaccaggacg tctcggtgac gatcaacacg gacaacccgg
     4381 ggctgttcgc gatcgacctg agcggcgagc tcgaggtctg cgtcgacgtg ctcgggttct
     4441 ccgacgacga ccttcgccgc gtgaccgcga acgcactcga ggcgagcttc gtcggcaagt
     4501 cggccaaggc cgacgttcgc gaacggcact tcggctgggt ggacgcgtaa cctcagcgga
     4561 gcgcgctgag cgccttccac tggtcgagcg tgatgccggg gaacgtgtag tagctcgcgc
     4621 cgatcacgtc ccgctcgagg acggtctgga cgaatcccag cgtctcgggt ccggtggcgt
     4681 cgaacgagat ccccccgacc acgtggatcg ggacacgcag cccggaggtc tgccggcgga
     4741 tgatctggac gctttgcgac gtgtaccagg ccgcggcccg cggtccctcg acccggtacg
     4801 tgtagtacgt catcggcagg aacacgtcgt aggtctcggc gagctggcgc cacgggaagc
     4861 ccggccagaa atgaggatcg gtccggacca tgcggtcggg cgaagtcacg atcgcgccga
     4921 gcggatactc ggtgcccgcg gcacgccgga tggcggccga catgcggagc agccgagccg
     4981 tgcggaggct cggccggcga acctccgccg actcgatgtc gagcgcgaac gagtcgaacc
     5041 cgttgccgct gcgcgtctcg aaccggatcg tccggacggc ccggtgcagg tcgagccgga
     5101 ggtcgcggaa ccccggcagg taccacgcca ccacctcgag acgccgtcgg tgcgcggcgt
     5161 cgacgatcgc ccgcacgccc tccgggtaca cgaacggccg gtgtcggtgg tagttggagc
     5221 tctgcaggta gatcgtccgg atgccgtgcg cggccatggc ccggaccgcg tgcaccgggt
     5281 gctcccacac gtccgtgtcg tagatgtcga tccacgtgcc gagcccctcg tacggcttcg
     5341 tggccgaggc ggaaccgcgc gtcccgttcg caggctcgag ccatcgattc gtcgaccgat
     5401 cgggcgaccc gtgccgcccc cccgccgatc cgacgatctc gggcgaaccc ccgatgcacg
     5461 cggcgagccc cgccagacaa agcagcgcgt acgcgcgcgc gaccctcgac ctcatgcacc
     5521 ttccccctgc aacgccggtc ccgaagaaca gagaacgttc ggaatgcccc aaggttcggt
     5581 gccatcggtg acgggctcgg atcggtcggg ctcggccggg atcgccgggc aggcaggccg
     5641 cgcccgctag gatgggcgct ccttcggagg acctcgggag cgaacggaca atgggagacg
     5701 acggcatcgc ggcggaccgg ctgctcgaac gctattggga ggggctgctc gaggtcg
//
LOCUS       DATBRK010000034         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_436467, whole genome shotgun sequence.
ACCESSION   DATBRK010000034 DATBRK010000000
VERSION     DATBRK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436467"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VIH70_01090"
     CDS             <1..495
                     /locus_tag="VIH70_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8114987.1"
                     /translation="LFCSGLLQALSSIASGWLATRIGLLPTMVFTHLPSNVLLILIPV
                     MPTLGSAIAMLLLRSTLSQMDVPARQAYVTALVDPDERTAAAAFTNTARYVVRPFGAA
                     GAGLLMQSVAAGAPFVAAGVLKSVYDILVFARFRRVPLPDERRGGRRDPKTETEIPAE
                     PPGR"
     gene            complement(496..>1822)
                     /locus_tag="VIH70_01095"
     CDS             complement(496..>1822)
                     /locus_tag="VIH70_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY8114988.1"
                     /translation="AEERDHIVKGLLIALDNLDAVIKVIRGSQDAEEARGKLMSQFKL
                     SEVQANHILDMPLRRLTRLARAELEQEHKDLLARIRHLKALLKDPKKIRGVITDEVLE
                     IKKKFANPRRTQLKADEGDLDVQDLIAEEAVVITVSRAGYVKRQPIENFRRQGRGGRG
                     IRGANLKEEDVINDVFTTTTHNWLLFFTGKGKVYRVKVHEVPESGRTARGTYAANLPG
                     VSITGDEKVQAVIDLKEYEEGRYLLFATRKGMVKKTTLPEYDSPRTGLAAINLRPGDE
                     LIDVRLTDGKDEVFLVSRTGQAIRFKEGLVRPMGRQTAGVIGMRLAPDDEVIVLGVAS
                     QGEELISVTHQGYGKRTPLRDYPVKGRGGKGVIGHTLTKKTGLLAGAFVGSKDQDMFV
                     ISSSGIVIRVAAADIRRVGRASQGVRTMRVEEGATVVALAPVIAAAELE"
BASE COUNT          232 a          656 c          594 g          340 t
ORIGIN      
        1 ctgttctgtt cgggtctgct ccaggcgctc tcgtcgatcg cgtcgggatg gctcgccacc
       61 cggatcggcc tgctcccgac gatggtgttc acccacctgc cgtcgaacgt cctgctgatc
      121 ctgatcccgg tgatgccgac gctcggctcg gcgatcgcga tgctgttgct gcgctcgacc
      181 ctgtcgcaga tggacgtccc ggctcggcag gcctacgtca cggcgttggt cgatcccgac
      241 gaacgtaccg cggcggcggc gttcaccaac accgcgcgct acgtcgtacg tcccttcggc
      301 gccgccggcg cgggcctgct gatgcagtcg gtcgcggccg gtgcgccgtt cgtggcggcg
      361 ggggtcctga agagcgtcta cgacatcctg gtgttcgcgc ggttccggcg ggtccccctc
      421 cccgacgagc gtcgcggcgg ccgaagggat ccgaagacgg agacggagat ccctgcggag
      481 ccgccgggcc ggtagctact cgagctcggc tgcggcgatg accggcgcga gcgcgacgac
      541 ggtcgcaccc tcctccacgc gcatcgtccg aacaccctgc gaggcgcggc ctacgcggcg
      601 gatgtccgcg gccgcgaccc tgatcacgat cccggaggac gagatcacga acatgtcctg
      661 gtccttcgac ccgacgaacg cgccggcgag caggccggtc ttcttcgtga gcgtgtggcc
      721 gatcacgccc ttgcctccgc gccccttcac cgggtagtcc cgcaggggcg tccgcttccc
      781 gtacccctgg tgcgtgacgc tgatcagctc ctcgccctgc gatgcgacgc cgaggacgat
      841 cacctcgtcg tccggcgcca ggcgcatccc gatcacaccc gcggtctgcc ggcccatcgg
      901 ccgaacgagg ccctccttga accggatcgc ctggcccgtg cgcgagacca ggaacacctc
      961 gtccttgccg tcggtcaggc ggacgtcgat cagctcgtcg ccgggcctca ggttgatcgc
     1021 ggcgagcccg gtccgcggcg agtcgtactc gggcagggtc gtcttcttga ccatcccctt
     1081 gcgcgtcgcg aacaggaggt accggccctc ctcgtactcc ttcaggtcga tcacggcctg
     1141 gaccttctcg tcccccgtga tcgagacccc gggcaggttc gcggcgtagg tccctcgcgc
     1201 ggtccgcccg gactccggca cctcgtggac cttcacgcgg tagaccttcc ccttgccggt
     1261 gaagaacagg agccagttgt gcgtcgtcgt cgtgaagacg tcgttgatca cgtcctcttc
     1321 cttgaggttc gcgccgcgga tgcccctgcc gccccgcccc tgccggcgga agttctcgat
     1381 gggttggcgc ttcacgtagc cggcgcgtga cacggtgatg accacggcct cctcggcgat
     1441 caggtcctgc acgtcgaggt cgccctcgtc ggccttgagc tgggtacgcc gggggttcgc
     1501 gaacttcttc ttgatctcga gcacctcgtc cgtgatgacg cctcggatct tcttcgggtc
     1561 cttgagcaga gccttcaggt ggcggatccg cgcgagcagg tccttgtgct cctgttcgag
     1621 ctccgccctc gcaagccggg tgaggcgtcg gagcggcatg tccaggatgt ggttcgcctg
     1681 cacctcgctg agcttgaact gcgacatcag cttgccgcgc gcctcctcgg cgtcctgcga
     1741 accacggatc accttgatga ccgcgtcgag gttgtcgagc gcgatcagca ggcccttgac
     1801 gatgtggtcg cgttcctcgg cc
//
LOCUS       DATBRK010000035         3694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127 k141_3636,
            whole genome shotgun sequence.
ACCESSION   DATBRK010000035 DATBRK010000000
VERSION     DATBRK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3636"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..636)
                     /gene="scpB"
                     /locus_tag="VIH70_01100"
     CDS             complement(73..636)
                     /gene="scpB"
                     /locus_tag="VIH70_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010476081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY8114989.1"
                     /translation="MNDRASSLRSLEALLFISDEPVASAVLAQALGAERPEVDAWCEE
                     LARGYEEREAGLTLRNVAGGWRLTTHPEAAPVVEQFVLASRHVRLTKAALETLSIVAY
                     KQPVTRHQISGIRGVNSEGVLRALVDRGLVAEVGREETPGRPVLYGTTPEFLERLGLP
                     SLSALPALAPLLRAGAGDAEAETADTD"
     gene            complement(633..1403)
                     /locus_tag="VIH70_01105"
     CDS             complement(633..1403)
                     /locus_tag="VIH70_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY8114990.1"
                     /translation="MTPTAGPGSAERPEGGAFAVDLPVFTGPFRVLADLILEQKIDVC
                     DVSVATVTDGFLAHSKAPETWSLEEATWFLAVCAIMLELKVGRLMPRHEVVDEEDLLG
                     VSPDLVYARSIELAAFRTVALEIARGLDGEGLRFTREAGPPAEFAHLYPDVLAGVSAD
                     DLARIAAVVLRPPPLVDLSHVTPIRYSMAEAVEAVRDHLGRLGQASFRDLVADCDERI
                     QIVVRFLALLELYREGMVDLAQAATFGEIHVWWEGEAA"
     gene            complement(1400..2431)
                     /gene="trpS"
                     /locus_tag="VIH70_01110"
     CDS             complement(1400..2431)
                     /gene="trpS"
                     /locus_tag="VIH70_01110"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY8114991.1"
                     /translation="MGDAATGTGGKIRIFSGIQPTGSKHLGNLIGGVRQYVEGQEVGD
                     SIYCLVDLHAITMKHDPAALRRQVHDTVSILIAAGLDPDRCILFRQSDVAEHTYLCWL
                     LASVTAYGELGRMTQFKDKSAEQKEFVSAGLFLYPVLQAADILAYQTDEVPVGEDQKQ
                     HIELARDVAERFNSRYGQMFTIPRHRIPEVGARILDLQDPDKKMSTTGGSEQGTVYIL
                     DPPDAIARKFKSAVTDSGREIRRAPDKRGISNLVEIMAVARGVTPGDIEREYASDPGY
                     ARFKDDVGSAVAEYLRPIRTAFDAIRSDESRLESILREGAAKARAIAAKTVETAADRM
                     GLGAGSGAG"
     gene            complement(2475..3122)
                     /locus_tag="VIH70_01115"
     CDS             complement(2475..3122)
                     /locus_tag="VIH70_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY8114992.1"
                     /translation="MDDLKFAVYLGISLVPALVLHEYAHAFVAVRLGDPTAKRWGRLT
                     LNPRPLIDPFGSLILPALALILVAARGVLLLPVFAYAKPMPVDPTMFKNPRRDTNLVV
                     LAGLLANIALAFLGGLPIRAGVTGELGTFALAWLIVNAFMFGFQLMPVPGLDGAKLLA
                     PFLPPRPREVYVNAEQYLVLFILVIFFLLSGPLLSIVDTFANVACNLAAGIDCLG"
     gene            complement(3122..>3694)
                     /locus_tag="VIH70_01120"
     CDS             complement(3122..>3694)
                     /locus_tag="VIH70_01120"
                     /inference="COORDINATES: protein motif:HMM:NF019448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114993.1"
                     /translation="EAIGNALDVVEAVRLLRGELHGRLRDVAVMFAGEAVSRLSDVPM
                     GEGRARAAAALDSGEALEAFRAMVQAQGGDPRVVDDPEGVLPAAPVVRSFAAERAGTL
                     AAVDAEALGRASGELGAGRKRKGDPIDPAVGIVFLPKVGDRLEPGQELGRVHARSDAD
                     AQACVRSVLAAITHSDDPVEPPPLVYGWHG"
BASE COUNT          575 a         1356 c         1259 g          504 t
ORIGIN      
        1 ctcgcacgcg cgtcgcgacc cgaacccggc gcgggcgagc gaacgctgga gccgctcgac
       61 gggcatccct cgtcagtcgg tgtctgccgt ctcggcctcc gcgtcgcccg cacccgcgcg
      121 caggagcggc gcgagcgcag gcagggccga gagcgagggc agaccgagac gctcgaggaa
      181 ctccggggtc gtcccgtaca ggaccggacg ccccggcgtc tcctcgcgac ccacctcggc
      241 cacgagcccc cggtccacga gcgcccgaag gaccccctcc gagttcaccc cgcggatccc
      301 cgagatctgg tgccgcgtga ccggctgctt gtacgcgacg atcgacagcg tctcgagggc
      361 cgccttcgtc agccggacgt gccgcgaggc cagcacgaac tgctcgacga ccggcgcggc
      421 ctcggggtgg gtcgtgaggc gccacccgcc ggccacgttc cgcagtgtga ggccggcctc
      481 ccgctcctcg tagccgcggg cgagctcctc gcaccacgcg tccacctcgg ggcgttcggc
      541 ccccagggcc tgggcgagga ccgccgaggc gacgggttcg tccgagatga acaacagcgc
      601 ctcgagcgat cggagcgagg aggcccggtc gttcacgcgg cctcgccctc ccaccagacg
      661 tggatctcgc cgaacgtcgc ggcctgcgcc aggtcgacca tgccctctcg gtagagctcg
      721 agcagcgcga ggaaccgaac gacgatctgg atccgctcgt cacagtccgc gacgagatcg
      781 cgaaacgacg cctgcccgag ccggcccagg tgatcgcgga cagcctcgac ggcctcggcc
      841 atcgaatagc ggatcggcgt cacgtgggac agatccacga gcggcggcgg gcggagcacg
      901 acggccgcga tccgcgcgag gtcgtccgcg ctcacgcccg ccaggacgtc cgggtacagg
      961 tgcgcgaact cggccggcgg gccggcctca cgcgtgaacc ggagaccctc cccgtcgagc
     1021 ccccgcgcga tctcgagcgc gacggtgcgg aacgccgcca gctcgatcga acgcgcgtac
     1081 acgagatccg gcgacacgcc gagcagatcc tcctcgtcga cgacctcgtg ccgtggcatc
     1141 aggcggccga ccttgagctc gagcatgatc gcgcagaccg cgaggaacca cgtcgcctcc
     1201 tccagcgacc acgtctccgg cgccttcgag tgcgcgagga acccgtcggt gaccgtcgcg
     1261 accgagacat cgcacacgtc gatcttctgc tcgaggatca ggtcggcgag gaccctgaac
     1321 ggtcccgtga agaccgggag gtcgaccgcg aacgcgccgc cctccggtct ctcggcggac
     1381 ccgggaccgg ccgtcggcgt catcccgctc ccgagcccgc cccgaggccc atccgatcgg
     1441 ccgcggtctc gaccgtcttg gccgcgatcg cgcgggcctt tgcggcaccc tcgcgcagga
     1501 tcgactcgag acgcgactcg tcggagcgga tcgcgtcgaa ggccgtccgg atcggccgca
     1561 ggtactcggc gacggccgaa ccgacgtcgt ccttgaaccg ggcgtagccg gggtcgctcg
     1621 cgtattcccg ttcgatgtct cccggcgtga cgccgcgcgc gacggccatg atctcgacca
     1681 ggttggagat cccccgcttg tcgggcgccc gccggatctc cctgccggag tcggtgaccg
     1741 cggacttgaa cttccgtgcg atcgcgtcgg gtgggtcgag gatgtacacc gttccctgct
     1801 cggagccccc ggtcgtcgac atcttcttgt ccggatcctg gaggtcgagg atgcgtgccc
     1861 ccacctcggg gatccgatgt ctcgggatcg tgaacatctg cccgtagcgc gagttgaagc
     1921 gttccgcgac gtctcgagcc agttcgatgt gctgcttctg atcctcgccg accggcacct
     1981 cgtcggtctg gtacgcgagg atgtccgcgg cctgcagcac cggatacagg aagagccccg
     2041 ctgagacgaa ctccttctgt tccgcggact tgtccttgaa ctgggtcatc cggcccagct
     2101 cgccgtaggc ggtgacgctc gccagcagcc agcagaggta ggtgtgctcg gcgacgtcgc
     2161 tctgccggaa caggatgcat cgatccggat cgagcccggc cgcgatcagg atcgacacgg
     2221 tgtcgtggac ctgccgacgc agcgccgcag ggtcgtgctt catcgtgatc gcgtgcagat
     2281 cgaccaggca gtagatgctg tccccgacct cctggccttc gacgtactga cgcaccccgc
     2341 cgatgaggtt gcccaggtgc ttgctgcctg tcggctggat ccccgagaag atcctgatct
     2401 tcccgcccgt gcccgtcgcc gcgtcgccca cgatccggcg atgatagcgc cctggacggg
     2461 cggcttcggg agcgtcagcc gaggcagtcg atgccggccg ccaggttgca ggccacgttc
     2521 gcgaacgtgt cgacgatcga gagcagcgga ccggacagga ggaagaagat caccaggatg
     2581 aacaggacca gatactgctc ggcgttcacg tacacctcgc ggggccgggg aggcaggaac
     2641 ggggcgagga gcttcgcgcc gtcgagcccg gggaccggca tcaactggaa cccgaacatg
     2701 aacgcgttca cgatcagcca cgcgagcgcg aacgtcccga gctctccggt caccccggcc
     2761 ctgatcggca ggccaccgag gaacgcgagc gcgatgttgg cgagcaagcc ggccagaacc
     2821 accaggttcg tgtcgcgccg agggttcttg aacatcgtcg ggtccacggg catcggcttc
     2881 gcgtaggcga atacgggcaa caggagcacc ccccgggcgg cgaccaggat cagcgcgagc
     2941 gccggcagga tcagcgagcc gaacgggtcg atcaggggcc tggggttcag ggtgagccgc
     3001 ccccagcgct tcgcggtggg gtcgccgagc cgcaccgcga cgaaggcgtg cgcgtactcg
     3061 tgcaggacga gcgccggcac cagcgagatg ccgaggtaga ccgcgaactt caggtcgtcc
     3121 atcagccgtg ccacccgtac acgagcggcg gcggctcgac cggatcgtcc gagtgggtga
     3181 tcgcggccag gaccgatcgg acgcaggcct gggcgtccgc gtcgctgcgg gcgtgcaccc
     3241 gcccgagctc ctgaccgggc tcgaggcgat cgcccacctt cggcaggaac acgatgccga
     3301 ccgccgggtc gatcgggtcc cccttcctct tcctccccgc accgagctcg ccgctcgctc
     3361 gcccgagggc ctcggcgtcg acggccgcca gggtgccggc ccgctcggcc gcgaacgaac
     3421 gaaccacggg tgcggccggg agaacaccct cgggatcgtc cacgacgcgc gggtcgccgc
     3481 cctgcgcctg gaccatcgcg cggaacgcct cgagcgcctc ccccgagtcg agcgccgcgg
     3541 ccgcccgagc ccgcccctcc cccatcggga cgtccgagag ccgggacacc gcctcgccgg
     3601 cgaacatcac cgccacgtca cgcaggcgtc cgtgcagctc tccgcggagc aaccggacgg
     3661 cctcgaccac atcgagggcg ttcccgatcg cctc
//
LOCUS       DATBRK010000036         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1013850, whole genome shotgun sequence.
ACCESSION   DATBRK010000036 DATBRK010000000
VERSION     DATBRK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013850"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VIH70_01125"
     CDS             complement(<1..1578)
                     /locus_tag="VIH70_01125"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8114994.1"
                     /translation="MGVLSGPVTVCIDRPLLALDRPFTYDLPGELGAGIGSVVQVPFH
                     GRATRAWVLGATDDVPERMLRVRRLVSPVRSFDERSLALFRAMSEQYVAPLATVIGRA
                     VPPRVASEETSVETPPPGGSPLRAPGTSPIEAYRDGPRLLGGLRAGQGGAFVVRPAPT
                     DEADAAAACVAATLAAGLTAIVIVPDADPVPATAAAVIDAFGDDAVLFLGGDKRVRYR
                     TWLEIAAGRYRVVVGTRPAVFAPLAELGLIYVHREGHPQHRDERSPSFHVRDVARMRA
                     EIVGAVSVLSAFCPSLEATARDHVLVEPAGRPWAPVEVVRPGPEGRSPRLIRALRSAT
                     RAFVFEPLRGYGVARVCRSCGEPAACASCLGMLRLERGIVRCVVCEAPGRCASCGATD
                     FGIARRGAERVEEWARGVATVPVSLIGRDDPARAPIEREVLVGGVDALKDVGSPGLDL
                     VAILHADASLRRPGVDARERVLVTWFEAAAWARSDGRVIVQTDAPNDPAVQALVSGRP
                     DRFRRAEAPRRAEAGFPV"
     gene            complement(1622..>2422)
                     /gene="metK"
                     /locus_tag="VIH70_01130"
     CDS             complement(1622..>2422)
                     /gene="metK"
                     /locus_tag="VIH70_01130"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY8114995.1"
                     /translation="DQGMMFGYACRETDELMPLPIAMAHRFAKRLADVRKAGVIPYLR
                     PDGKSQVTIAYEGTTAKRVDTVLISAQHREEVDVETLLAPDVRSEVIDPVLREFELDA
                     EGVRILVNPTGRFEIGGPKADTGLTGRKIIVDSYGGMAPHGGGCFSGKDPTKVDRSAA
                     YMARYVAKNLVAAGSADRLQIQVAYAIGTAHPVSLAVDTFGTEQVDPARLVDVVWDAF
                     DFRPAAIIRDMDLRRPIYEDTAAYGHFGRKEFPWEATDRADELRRALG"
BASE COUNT          347 a          914 c          866 g          295 t
ORIGIN      
        1 caccgggaag ccggcctcgg cccggcgcgg ggcctcggcg cgtcggaacc ggtcgggtcg
       61 tcccgagacg agggcctgga ccgcggggtc gttcggggcg tcggtctgca cgatgacccg
      121 tccgtccgaa cgggcccagg ccgccgcctc gaaccacgtg acgagcaccc gctcacgagc
      181 gtccacgccg ggacgacgga gcgaggcgtc ggcgtgcagg atcgccacga ggtccaggcc
      241 cggagagccc acgtccttca acgcgtcgac cccgcccacc agcacctcgc gttcgatcgg
      301 cgcgcgggct gggtcgtcgc gaccgatcag ggacaccggc acggtggcga cgccgcgcgc
      361 ccactcctcg acccgttcgg cgccgcgacg ggcgatgccg aagtcggtcg caccgcagga
      421 cgcgcaccgt ccgggcgcct cgcataccac gcaccgaacg atgccgcgtt cgagccgcag
      481 catcccgagg cacgacgcac acgcggccgg ttccccgcag gacctgcaga ccctggcgac
      541 cccatagcca cgcagcggct cgaacacgaa cgcgcgagtg gccgagcgga gcgcccggat
      601 cagtcgaggc gaccggccct cgggaccggg ccgaacgacc tcgaccggcg cccacggcct
      661 gccggccggc tcaacgagga catggtcacg ggccgtggct tcgagcgagg ggcagaacgc
      721 gctcaggacg ctcacggccc ccacgatctc ggcccgcatc cgggccacgt cacggacgtg
      781 gaacgacggc gagcgctcgt cccgatgctg cgggtgtccc tcacgatgca cgtagatcag
      841 tcccagctcg gcgagcggcg cgaacaccgc gggacgcgtg cccaccacca cccggtagcg
      901 gccggcggcg atctcgagcc aggtgcggta ccggacgcgc ttgtcgcccc ccaggaacag
      961 caccgcgtcg tctccgaacg catcgatgac cgccgccgcg gtcgcgggca cggggtccgc
     1021 gtccggcacg atcacgatcg ccgtgagccc ggcggcgagc gtcgcggcga cgcaggccgc
     1081 ggcggcgtcc gcctcgtcgg tgggcgcggg ccgcaccacg aatgcgccgc cctgccccgc
     1141 ccgaaggccc cccaggagtc gtgggccgtc ccggtaggcc tcgatgggtg aggtccccgg
     1201 cgcccgcagg gggctccccc ccggcggcgg ggtctcgacc gaggtctcct ccgaggcgac
     1261 gcgcgggggt accgcgcggc cgatcacggt cgcgagcggc gcgacgtact gctcgctcat
     1321 cgcgcggaac agcgcgagcg agcgctcgtc gaaggatcgg acgggcgaga cgagcctgcg
     1381 tacccggagc atcctctcgg gcacgtcgtc ggtcgcgccg agcacccacg cgcgcgtggc
     1441 ccggccgtgg aacggcacct gcaccacgga cccgataccg gcgccgagct ccccggggag
     1501 gtcgtaggtg aagggacgat cgagggcgag cagcgggcgg tcgatgcaga cggtgaccgg
     1561 gcccgacagg acccccatcg gcgactcccg cggtgcgctc ggggcgaacc gggagctcgc
     1621 gctagccgag cgcgcggcgg agctcatccg cgcggtctgt cgcctcccag gggaactcct
     1681 tgcgcccgaa gtggccgtag gcggccgtgt cctcgtagat cgggcgtcga agatccatgt
     1741 cccggatgat cgccgccggc cggaagtcga acgcgtccca cacgacgtcc acgaggcgcg
     1801 cggggtcgac ctgctccgtc ccgaacgtgt cgaccgcgag cgagaccggg tgggccgtcc
     1861 cgatcgcgta cgccacctgg atctgcagcc ggtcggccga gccagcggcc acgaggttct
     1921 tcgcgacgta ccgcgccatg tacgccgccg agcggtcgac cttggtcggg tccttgcccg
     1981 agaagcagcc gccgccgtgc ggcgccatcc ctccatagct gtcgacgatg atcttccggc
     2041 ccgtcagacc ggtgtctgcc ttggggccac cgatctcgaa ccggcccgtc gggttgacga
     2101 ggatgcggac gccctcggcg tccagctcga actcgcgcag caccgggtcg atcacctcgg
     2161 agcggacgtc tggggcgagg agggtctcga cgtcgacctc ctcgcggtgc tgtgcgctga
     2221 tcagcacggt gtcgacccgc ttggcggtcg tcccctcgta cgcgatcgtg acctggctct
     2281 tgccgtccgg ccgcaggtac gggatcaccc ccgccttgcg cacgtcggcc agccgcttgg
     2341 cgaaccggtg cgccatcgcg atcggcagcg gcatcagctc gtcggtctcg cggcaggcgt
     2401 acccgaacat catcccctgg tc
//
LOCUS       DATBRK010000037         8486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2459285, whole genome shotgun sequence.
ACCESSION   DATBRK010000037 DATBRK010000000
VERSION     DATBRK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2459285"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VIH70_01135"
     CDS             complement(<1..1041)
                     /locus_tag="VIH70_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8114996.1"
                     /translation="MNKTVGTGFVVLAALAIVAAACGNDDNTPSGETGGAIEPAKTYD
                     SIGETEGSLELIAWAGYVEDGTSEGGEDFDWVTPFEDETGCQVNVTYGDTSDEMVTLM
                     RQGGGTVYDGVSASGDASNRLIAGGDVGAIDPSLFPQFANVIGPLNPDGGTNNEHYVV
                     DGNVYGTPYMYGPNFLMYNTEEVTPAPTSWDVTFEPDSPYAGSITVYDSPIFIADAAM
                     YLMAHNPDLGITDPYELSQEQFDAAVELLKAQAGMVVRPWALYTDEIDGFVDGSMLVG
                     TAWPINLSLAELDAPVDAVIPSEGVTGWADTWMISANAPHPNCMLKWMDWTMQPDVQA
                     EVAVWYGAAGSNT"
     gene            complement(1038..2159)
                     /locus_tag="VIH70_01140"
     CDS             complement(1038..2159)
                     /locus_tag="VIH70_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8114997.1"
                     /translation="MAQRATRADRALAGAPTASAVTLESVVKRFGDVVAVDGVDLEVR
                     EGEFFSMLGPSGSGKTTCLRMIAGFEAPTSGRVLLGGEDVSHLAPYERDVNTVFQDYA
                     LFPHMTVEENVEYGLKVKKVPAVERRDRAADALAMVQLSAFGKRKPSQLSGGQRQRVA
                     LARALINRPKVLLLDEPLGALDLKLRQAMQIELKEIQGHVGLTFVYVTHDQEEALTMS
                     DRLAVFSRGRVEQIGTPAEVYERPATGFVAGFVGTSNVLEGEAAAAIAGDAHAFTIRP
                     EKIAMVEPDAEVPDGACTATGHVREVVYLGALTRYIVTLDVGGELVVMQQNLATSSME
                     ALQVRGKVVRLVWDRRNNRPVDAAGGSMDGSEDPGEDAA"
     gene            complement(2245..3243)
                     /locus_tag="VIH70_01145"
     CDS             complement(2245..3243)
                     /locus_tag="VIH70_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase 2"
                     /protein_id="HEY8114998.1"
                     /translation="MARPLLFSIPPEAAHRLAQGLLGLPLPWERLGGADRDPALRLSL
                     AGIPLANPVGLAAGFDKTGRHVDALGRLGFGYVVCGTFTRGPRRGNPKPRIVRYPERA
                     SMVNAMGLPNPGAQAASRTLGRTASPCPRVASVADQDLPDVLEAHAVLEPFVDAIELN
                     SSCPNVAWGRDRDNEAHLANLLRELAGRRVRPLFVKLPPFRTRVEREVVLALAAIARE
                     QGADALTISNTLPVHDARVSIGRGGLSGRALRDVTLACVRAVRGAMGPEMPVNASGGI
                     FTPADALAAIEAGATTVQVYTGLIFRGPGVIRELTAGLADALRSRRVDLAALVGTA"
     gene            3331..3837
                     /locus_tag="VIH70_01150"
     CDS             3331..3837
                     /locus_tag="VIH70_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY8114999.1"
                     /translation="MPGDLAERLIAASELHGDFVLSSGRRSNVYFDKFRFLTDPDLLR
                     DTARAVASLLPEGITHLAAPEGAATLLVAALALETGLPIAVVRREPKAYGTMSQLEGH
                     APAGAEVALVEDVSTTGVQVRSAASVLEHAGCKIGIIVLAIDRGGAEQLREAGYWVKS
                     VVEVDPGG"
     gene            complement(3863..4618)
                     /locus_tag="VIH70_01155"
     CDS             complement(3863..4618)
                     /locus_tag="VIH70_01155"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY8115000.1"
                     /translation="MSDDTAETMLEWKGRTGPFTVSLGRGVFHPTHTSRTIAEALEIE
                     PGDTVIDVGCGSGVLSFVAARLGAKRVIGTDLSEDAIEVAKQNAVRLGLQREVEFRSG
                     DLLDPVRGEQADVIIGDVSGIPDEIGRLAGWFPDGRAGGPTGAELPAAMLESIRVNEC
                     LAPGGHLYLPTGTIQAESKILDVARRIFGAANLQSLLSREFPLPDVVARSKALAQMMK
                     EGIVDLRMRGSRMLWRLQVWRCEMPEDGRLTTI"
     gene            complement(4671..5162)
                     /locus_tag="VIH70_01160"
     CDS             complement(4671..5162)
                     /locus_tag="VIH70_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEY8115001.1"
                     /translation="MREDAVGKTGRPTETRNKPRRPRDELGRPRPWDAENALELEDFD
                     ALTLEENHRLGREHLDAGRFFPAHEAWETAWKQARDTTDAEFFKGLSQLGAGYVHLLR
                     GNAHGAKRLLRRASGRIRAYPSGHHGVETIGVADAADALAADIDSGVVRPGEDAVFVP
                     PHV"
     gene            5251..6519
                     /locus_tag="VIH70_01165"
     CDS             5251..6519
                     /locus_tag="VIH70_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8115002.1"
                     /translation="MDPAAAQEPPAEPGPAEEPEPVLTDAPIGGAEVVRRPSVDGAMP
                     AWIPRLLLAVVITAFAAYAAFSLLRRLSHLLLWLVAALFLSFALEPAVNWLVAHGWKR
                     GVAAASVLLGLGLLGIIGVALMVPLVIDQVQELIARVPGWLDEASVYTQRWFDTRLTS
                     ATILRQVANAQADVRGIAANVASVGALLLGLVFQVLTIGLFTFYLVADGPRFRRGVCS
                     LLPPRVQRDLLAAWEVAIDKTGGYLYSRLLLAIINATFSFIMLQALGIPFALPLALWQ
                     GFVSQFIPVVGTYIAAAIPLLVALLEDPWKALWFLIFVIVYQQIENYLLAPRITARTM
                     QLHPAVAFGAALAGGSISGLLGAFMALPAAAVIQSTISTYLKRHEVVETELTREDAVF
                     IEERSSANDDRSNERGILGRLSARWRRPEA"
     gene            complement(6523..7209)
                     /locus_tag="VIH70_01170"
     CDS             complement(6523..7209)
                     /locus_tag="VIH70_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY8115003.1"
                     /translation="MAGEHERADDAFTAEEVRWADRYGLLFLLILVALVLTAANTTWL
                     KIAAVLVQGGVVLFAFLASRVGRRAWRVAMVLVPLAVLLGIAGRFGDSRPAEAVAAVM
                     NLVFPAAAIVALGRRIVVEPFVSGRTILGLLCVYLLVGMTFAALYITIAVVSGEPFFV
                     QTGAEPVDFTYFSLVTLATVGYGDFTAANPMPRMLAAIEGLTGQLYLVTVVAVAVSRV
                     RTRRDRTPEG"
     gene            7360..8187
                     /locus_tag="VIH70_01175"
     CDS             7360..8187
                     /locus_tag="VIH70_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY8115004.1"
                     /translation="MSPPDELSEQDRTTKDRLDILTQAVRRLRGRQWRVVPHPGPIGG
                     EQHVAGGRSGALRAAIFGVNDGLVSNLSLIFGVSGAGVDNSVVIVAGVAGLLAGAFSM
                     AAGEYISVRVQREVYERLIHLEAHEIGSDPEAERLELAELYVRKGLPRDLADRLATEL
                     MRDPEVALETHAREELGLDPREGLGSPFAAAGSSFVTFSLGAFIPLLPFLVSSGTPAV
                     VASAILSGISLFGVGAAMSYLTGRPWLLSGSRMLTVGSAAAGVTFLVGKALDVGVLS"
     gene            complement(8180..>8486)
                     /locus_tag="VIH70_01180"
     CDS             complement(8180..>8486)
                     /locus_tag="VIH70_01180"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY8115005.1"
                     /translation="VHLGMGDDGHTASLVPGDPVLHVTDRDVAVTGEYRGLRRMTVTY
                     PVLDRAGLALWLITGGDKAPMVPRLLAGDRSIPAGRVATAEQLVVVDAAAAAEVAPS"
BASE COUNT         1190 a         3061 c         2940 g         1295 t
ORIGIN      
        1 cgtgttgctg ccggccgcgc cgtaccagac cgcgacctcc gcctgcacgt cgggttgcat
       61 cgtccagtcc atccacttca gcatgcagtt cgggtggggg gcgttcgccg agatcatcca
      121 ggtgtccgcc caacccgtga ccccttcgga cgggatcaca gcgtccaccg gagcgtcgag
      181 ctcggcgagg ctgaggttga tcggccatgc cgtgcccacg agcatcgacc cgtccacgaa
      241 gccgtcgatc tcatcggtgt agagcgccca cggccgaacg accatccccg cctgcgcctt
      301 cagcagctcc accgccgcgt cgaactgctc ctgcgagagc tcgtacgggt cggtgatccc
      361 aaggtcgggg ttgtgcgcca tcaggtacat ggccgcgtcg gcgatgaaga tcggcgaatc
      421 gtagaccgtg atcgaccccg cgtagggcga gtccggctcg aacgtcacgt cccagctcgt
      481 gggggcgggc gtcacctcct cggtgttgta catcaggaag ttcggcccgt acatgtacgg
      541 ggtcccgtag acgttgccgt ccacgacgta gtgctcgttg ttggtcccgc cgtccggatt
      601 gagcggcccg atcacgttcg cgaactgcgg gaagagcgac gggtcgatcg cccccacgtc
      661 cccgccggcg atcagccggt tgctcgcgtc gccggatgcc gagacgccgt cgtacacggt
      721 gccgccgccc tggcgcatca gcgtcaccat ctcgtccgag gtgtcaccgt aggtcacgtt
      781 gacctggcat ccggtctcgt cctcgaaggg cgtgacccag tcgaagtcct cgccgccctc
      841 gctcgtcccg tcctcgacgt atccggccca cgcgatcagc tcgagcgagc cctccgtctc
      901 gccgatcgag tcgtaggtct tcgcgggctc gatcgctccc ccggtctcgc cgctcggggt
      961 gttgtcgtcg ttcccgcacg ccgccgccac gatggcgagc gccgcgagca cgacgaaccc
     1021 cgttcccacc gtcttgttca tgctgcgtcc tcccccgggt cttccgaccc atccatcgaa
     1081 ccgccggccg cgtcgaccgg gcggttgttc ctgcgatccc agaccaggcg cacgaccttg
     1141 ccgcgcacct ggagggcctc catcgacgac gtggcgaggt tctgctgcat cacgacgagc
     1201 tcgcccccga cgtccagcgt gacgatgtag cgggtcagcg cccccaggta gacgacctca
     1261 cggacgtgac cggtcgccgt gcaggcgccg tccgggacct cggcgtccgg ctccaccatc
     1321 gcgatcttct ccgggcggat cgtgaacgcg tgggcgtctc cggcgatcgc cgccgccgcc
     1381 tcgccctcca ggacgttgga ggtcccgacg aacccggcga cgaacccggt ggccggccgt
     1441 tcgtagacct cggccggcgt cccgatctgc tcgaccctcc cgcgactgaa cacggccagc
     1501 cggtcgctca tcgtgagcgc ctcctcctgg tcgtgcgtga cgtagacgaa cgtcaggccg
     1561 acatgcccct ggatctcctt cagctcgatc tgcatcgcct gccggagctt gaggtcgagg
     1621 gcgccgagcg gctcgtccag caggagcacc ttgggacggt tgatcagcgc gcgggccagg
     1681 gccacgcgct ggcgctgccc gcccgagagc tggctcggct tgcgcttccc gaaggccgac
     1741 agctgcacca tcgcgagcgc gtcggccgcg cggtcgcggc gctcgaccgc gggcaccttc
     1801 ttgaccttga ggccgtactc cacgttctcc tccaccgtca tgtgggggaa cagggcgtag
     1861 tcctggaaga ccgtgttcac gtcgcgctcg tacggggcga ggtgggagac gtcctcgccg
     1921 ccgagcagca cccggccgga ggtcggggcc tcgaaccccg cgatcatccg gaggcaggtg
     1981 gtcttgcccg acccggaggg accgagcatc gagaagaact caccctcgcg gacctcgagg
     2041 tcgactccgt ccacggcgac cacgtcgccg aaccgcttga cgaccgattc gagcgtgacc
     2101 gcggacgcgg tcggagcccc cgcgagagcc cgatcggccc tggttgcacg ctgcgccaaa
     2161 cccacctccg ggtgttcggt tccgttcgga atcgcgtcat cttgaacggg aagcggtcgc
     2221 cgggcaaccc acacccggcc cggctcaggc ggtgcccacg agggcggcga gatcgacccg
     2281 ccggctccgg agcgcgtccg cgagccccgc cgtgagctcg cggatcactc cgggaccgcg
     2341 aaagatcagg cccgtgtaca cctgcaccgt cgtcgccccc gcctcgatcg ccgcgagcgc
     2401 gtcggccggg gtgaagatgc cgccgctggc gttgaccggc atctccgggc ccatggcccc
     2461 gcgaaccgcc cggacgcatg ccaacgtcac gtcgcgcagc gcgcggccgg agagcccgcc
     2521 gcgcccgatc gacacccgcg cgtcgtggac cggcagcgtg ttcgagatcg tgagggcgtc
     2581 ggcgccctgc tcgcgggcga tcgcggcgag cgcgagcacc acctcgcgct cgacccgggt
     2641 gcggaacggc ggcagcttga cgaacagggg acgcacccgg cggccggcga gctcgcgcag
     2701 caggttcgcg aggtgggcct cgttgtcccg gtcgcggccc caggcgacgt tcgggcagct
     2761 cgagttcagt tcgatcgcgt ccacgaacgg ctccaggaca gcgtgggcct cgaggacatc
     2821 ggggaggtcc tggtcggcga cgctcgccac ccggggacat ggcgaagccg ttcggcccag
     2881 cgtacgcgag gccgcctgtg cgccggggtt cggcaacccc atcgcgttga ccatcgaggc
     2941 gcgctccggg taccggacga tcctcggctt ggggttgccg cggcgtgggc cgcgcgtgaa
     3001 cgtcccgcac acgacgtagc cgaaccccaa ccgaccgagc gcatcgacgt gccgcccggt
     3061 cttgtcgaag ccggccgcca ggccgaccgg gttcgcgagc gggatcccgg ccaggctcag
     3121 ccggagcgca gggtcgcggt cggccccgcc aagccgttcc cacgggagcg gcaggccgag
     3181 gagcccctgc gcgaggcgat gcgcggcttc gggcgggatc gagaacagga gcggccgcgc
     3241 caccgctccg taccagtcac ttcctcggcc ggttggtcgg ccatccttcg tccctcgggc
     3301 acggcccatc gggggcctat gctacgcgcc atgcccggcg acctcgcgga acgtctgatc
     3361 gcggcctcgg agctccatgg cgacttcgtg ctctcctccg ggcgccgttc gaacgtgtac
     3421 ttcgacaagt tccgcttcct caccgacccg gacctgctga gggacacggc ccgggccgtc
     3481 gcctcgctgc tccctgaggg gatcacccac ctcgcggcgc ccgagggggc cgcgacattg
     3541 ctcgtggcgg cgctcgcact cgagaccggc ctgccgatcg cggtggtccg cagggagccg
     3601 aaggcgtacg ggacgatgtc gcagctcgag ggccacgcgc ccgccggcgc ggaggtcgcg
     3661 ctcgtggagg acgtgtcgac gaccggcgtg caggtccgtt ccgccgcctc ggtgctcgag
     3721 cacgccggct gcaagatcgg catcatcgtg ctcgcgatcg accggggggg cgcggagcag
     3781 ctgcgcgagg cgggctactg ggtgaagtcg gtggtggagg tcgacccggg cgggtgaggc
     3841 cccggccgac ggcggggccg acctagatcg tggtgaggcg gccgtcctcg ggcatctcgc
     3901 accgccagac ctggagacgc cacagcatcc ggctcccgcg catgcggagg tcgacgatgc
     3961 cttccttcat catctgcgcg agggccttgg acctggccac cacgtcgggg agcgggaact
     4021 cccgcgagag caacgactgc aggttcgcgg cgccgaagat ccggcgggcg acgtcgagga
     4081 tcttcgactc ggcctggatc gttccggtcg gcaggtacag gtgaccgccc ggcgcgaggc
     4141 actcgttcac acggatgctc tcgagcatcg ccgcggggag ctcggccccc gtagggccgc
     4201 cggcgcggcc gtccgggaac catccggcga gcctgccgat ctcgtcgggg atcccggaca
     4261 cgtcaccgat gatgacgtcc gcctgctcac cgcgcacggg gtcgagcagg tccccactgc
     4321 ggaactcgac ctcgcgctgc aggcctagac gaacggcgtt ctgcttggcg acctcgatcg
     4381 cgtcttccga gaggtccgtg ccgatcaccc gcttggcacc gagcctcgca gccacgaatg
     4441 agagcacgcc gctcccgcag ccgacgtcga tgacggtatc gccgggctcg atctccagcg
     4501 cctcggcgat cgtccggctg gtgtgcgtgg gatggaacac gccccgcccc agcgagaccg
     4561 tgaacggccc ggtgcggccc ttccactcga gcatggtttc ggcggtgtcg tcgctcatcc
     4621 gcatatcgtg gcggctgcgg gcagggacag tcaaccatcg aggcacgaac tcagacgtgc
     4681 ggaggcacga acacggcgtc ctccccgggt cggaccacgc ccgaatcgat gtccgccgcg
     4741 agcgcatcgg ccgcatcggc gaccccgatc gtctccaccc cgtggtgacc cgatgggtac
     4801 gcgcggatcc gtcccgatgc tcgtcggagc aaccgcttgg ctccatgcgc gtttcccctg
     4861 agcaggtgca cgtagcccgc gcccagctgg gacaggccct tgaagaactc cgcgtcggtg
     4921 gtgtcgcgcg cctgcttcca cgccgtctcc cacgcctcgt gggcggggaa gaagcgaccg
     4981 gcgtcgaggt gctcccgacc cagacgatgg ttctcttcga gcgtgagcgc gtcgaagtcc
     5041 tcgagctcga gcgcgttctc cgcgtcccag ggccgggggc gacctagctc gtcgcgcggc
     5101 cggcggggct tgttgcgggt ctcggtcggc cgaccggtct tgcccacagc gtcctcgcgc
     5161 atgcacccat cgtacggccg ccgcaaccga ccgccgtggt cgcgttcgcc gatgctggcg
     5221 cccgcattcg tcggccgata cgcttccggg atggatccgg ccgccgcgca ggagccgccg
     5281 gccgaacctg ggcccgccga ggaacccgaa cccgtcctca ccgatgcgcc gatcgggggc
     5341 gcggaggtgg tccgccgtcc gtccgtcgac ggtgcgatgc ccgcatggat cccgcggttg
     5401 ctcctcgccg tggtcatcac ggcgttcgcc gcctacgcgg cgttcagcct cctcaggagg
     5461 ctctcgcacc tgctcctgtg gctcgtcgcc gcgctcttcc tctccttcgc cctcgaaccg
     5521 gcggtcaact ggctggtggc ccacggctgg aagcgaggcg tcgcggcggc ctcggtgctg
     5581 ctcggcctcg gcctgctggg gatcatcggc gtcgcgctca tggtcccgct ggtgatcgat
     5641 caggtgcagg agctgatcgc acgcgtcccg gggtggctcg acgaggccag cgtctacacg
     5701 caacgatggt tcgacaccag gctgacgagc gcgacgatcc tgaggcaggt cgcgaacgca
     5761 caggcggacg ttcgcggtat cgcggcgaac gtcgcctcgg tgggagcgtt gctgctcggg
     5821 ctggtcttcc aggtcctcac gatcggcctg ttcacgttct acctcgtggc cgacggcccg
     5881 agattccggc gcggcgtgtg ctcgctcctg ccaccccgcg tccagcgcga tctcctcgcg
     5941 gcctgggagg tcgcgatcga caagaccggt gggtacctgt actcgaggct cctgctcgcc
     6001 atcatcaacg cgacgttctc gttcatcatg ctccaggcgc tcgggatccc gttcgcgctg
     6061 ccgctcgccc tgtggcaggg gttcgtctcg cagttcatcc cggtggtcgg cacctacatc
     6121 gccgcggcga tcccgttgct cgtggcgttg ctcgaggacc cgtggaaggc gctctggttc
     6181 ctgatcttcg tgatcgtcta ccagcagatc gagaactacc tgctcgcccc ccgcatcacg
     6241 gccaggacga tgcagctcca tccggcggtg gcgttcggag cggcgctcgc agggggctcg
     6301 atcagcgggc tgctgggggc gttcatggcg ctgcccgccg cggcggtgat ccaatccacg
     6361 atctcgacgt acctcaagcg gcacgaggtc gtcgagaccg agctcacgcg ggaagatgcc
     6421 gtgttcatcg aggagcggtc gtcggcgaac gacgaccgat cgaacgagcg cgggatcctc
     6481 ggtcgcctct ccgcccggtg gcggcgaccg gaggcctgaa cgtcagccct ccggcgttcg
     6541 atcccgtctc gtgcggaccc gcgacaccgc caccgccacg accgtgacca ggtagagctg
     6601 gccggtcagg ccctcgatcg cggcgagcat ccggggcatc gggttcgccg cggtgaaatc
     6661 gccgtacccg acggtcgcga gcgtgacgag actgaagtac gtgaagtcca ccggctcggc
     6721 accggtctgg acgaagaacg gttcgccgct caccaccgcg atcgtgatgt acaaagcggc
     6781 gaaggtcatc ccgaccagca ggtagacgca gagcaggccg aggatggtcc ggccgctcac
     6841 gaacggctcc accacgatgc gacggccgag cgcgacgatc gcggccgcgg ggaagacgag
     6901 gttcatgacg gcggccacgg cctcggccgg cctcgaatcg ccgaaccgtc cggcgatgcc
     6961 gaggagcacg gcgagcggca ccaagaccat cgcgacccgc cacgcgcgcc gcccgacccg
     7021 ggaggcgagg aacgcgaaca gcaccacgcc gccctgcacg aggaccgcgg cgatcttcag
     7081 ccaggtcgtg ttcgcggccg tgaggacgag cgcgaccagg atgagcagga atagcagccc
     7141 gtagcgatcg gcccagcgca cctcttcggc ggtgaaggcg tcgtcggcac gttcgtgttc
     7201 gcccgccacg cgcgaaccgt actacggcct cctcggccgt cgaggaggtc cgggttcgac
     7261 aggccacccg ccgcagacgg acaatcgcgc ggccggcgaa ccggagtagg agtcctcgat
     7321 gagcagcgtc gccatcaccg acgggtagat ctggccacga tgtcgccacc cgacgagctc
     7381 tctgagcagg accgcaccac gaaggatcgt ctggacatcc tcacgcaggc cgtccgacgg
     7441 ctccgcggac gacagtggcg ggtcgtcccc catcccggcc cgatcggcgg ggagcagcac
     7501 gtggcgggcg gcaggtcggg cgcgctccgg gcggcgatct tcggcgtgaa cgacggcctg
     7561 gtctcgaacc tctcgctgat cttcggcgtc tcgggagcgg gcgtcgacaa ctccgtcgtg
     7621 atcgtggcgg gcgtcgcggg gttgctggcg ggcgccttct cgatggcggc gggcgagtac
     7681 atctcggtgc gggtgcagcg cgaggtctac gagcggctga tccacctgga ggcgcacgag
     7741 atcggctcgg acccggaggc cgaacgcctg gagctcgccg agctctacgt gcgtaagggc
     7801 ctgccgaggg acctcgccga ccggctcgcg accgagctca tgagggaccc cgaggtggcg
     7861 ctcgagaccc acgcccgcga ggagctgggt ctggatccgc gcgagggcct cgggtcgccg
     7921 ttcgcggcgg cggggtcgtc cttcgtgacg ttctcgctgg gcgcgtttat cccgctcctg
     7981 ccgttcctcg tgtcgtccgg cacgccggcg gtcgtcgcct ccgcgatcct gtccggcatc
     8041 tcgctgttcg gcgtcggggc ggcgatgtcg tacctgaccg ggcgtccgtg gctcctgtcg
     8101 ggatcccgga tgctcacggt gggttcggcg gccgcgggcg tcacgttcct cgtcggcaag
     8161 gcgctcgacg tcggggtgct cagctagggg cgacctcggc cgccgcggcg gcgtcgacga
     8221 cgacgagctg ctccgcggtc gcgacccggc ccgcggggat cgaacggtcg cccgcgagca
     8281 agcgcggcac catgggcgcc ttgtcgcccc cggtgatcag ccagagggcc aggccggccc
     8341 ggtcgaggac gggataggtc accgtcatcc gccggagacc ccggtactcg cccgtcaccg
     8401 ccacatcgcg gtccgtcacg tggaggaccg ggtcgcccgg cacgagcgac gcggtatggc
     8461 cgtcgtcgcc catcccgagg tgcacg
//
LOCUS       DATBRK010000038         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2605169, whole genome shotgun sequence.
ACCESSION   DATBRK010000038 DATBRK010000000
VERSION     DATBRK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2605169"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VIH70_01185"
     CDS             <1..454
                     /locus_tag="VIH70_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEY8115006.1"
                     /translation="RAILDRRGITVVTNALNIAGELAIRADLRLVVTGGTARSESYEL
                     VGPVAEQALERLNLDVAFVGVDGISLEAGLTTHHEIEAHTNRTMIERARLVVVVADSS
                     KLGRVAFAQICPPDGIDELITDEGAAQATIRELTEAGVAVTVVTGAGT"
     gene            complement(451..1764)
                     /gene="melA"
                     /locus_tag="VIH70_01190"
     CDS             complement(451..1764)
                     /gene="melA"
                     /locus_tag="VIH70_01190"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEY8115007.1"
                     /translation="MAKVTIIGAGSVEFTRGILADLCAYEELHGSLEIALHDIDAERL
                     AYALMGADQIVERSNAGYEVSAHESRASAFDGADYLINEIQVGGYPATRTDFDVPKTF
                     GLRQTIGDTIGIGGIMRGLRTIPVMIEMADEMVERCPDGLLLNYTNPMAMVPWGIWSG
                     SRLPAANVVGVCHSVRDTHAFLARTIGAPERRVEFRTAGFNHQCFVYVFRDRESGEDL
                     YPRLRAIVDADPEGLGRRVRVELFKRFGYFPTESSEHGAEYVPWFLHDDEQIERYRIE
                     VDEYLRRSEENLAEWEGIRRALDAGEELEVERNDELASQFVRALETGAETELYGNVRN
                     DGSIEGLPDDACIEVPVRVGRDGVVPSRFGAIPPQCLALNRTFLNVVELTVRAVLEGS
                     RRLVYQAALVDPNTAATLTVEAIVEMVDELIDAHGELIPEAIRRG"
BASE COUNT          290 a          698 c          689 g          308 t
ORIGIN      
        1 gcgggcgatc ctggaccggc gcgggatcac cgtcgtcacc aacgccctca acatcgcggg
       61 cgagctcgcg atccgcgccg acctgaggct cgtggtcacg ggcggcaccg cacgctccga
      121 gtcctacgag ctggtcggtc ccgtcgcgga gcaggcgctc gagcgcctga acctggacgt
      181 cgcgttcgtc ggcgtcgacg gcatctcgct cgaggccggg ctgaccacgc atcacgagat
      241 cgaggcccat accaaccgga cgatgatcga gcgggctcgc ctggtcgtcg tggttgccga
      301 cagctccaag ctcggacggg tcgcgttcgc gcagatctgc ccgccggacg ggatcgacga
      361 gctgatcacg gacgagggcg ccgcccaggc gacgatccgc gagctcacgg aggccggcgt
      421 cgcggtgacc gtcgtgaccg gcgcggggac ctagccgcgg cggatcgctt cggggatcag
      481 ctcgccgtgc gcgtcgatga gctcgtccac catctcgacg atcgcctcga ccgtgagggt
      541 ggcggccgtg ttcgggtcga cgagcgcggc ctggtagacg agccggcgcg aaccctcgag
      601 gaccgcgcgg accgtgagct cgacgacgtt caggaacgtc cggttgagcg ccaggcattg
      661 gggcgggatc gcgccgaacc gcgacggcac gacgccgtcg cggccgaccc gcaccggcac
      721 ctcgatgcac gcgtcatcgg gcagaccctc gatcgatccg tcgttgcgca cgttcccgta
      781 gagctctgtc tcggccccgg tctcgagcgc ccggacgaac tggctcgcga gctcgtcgtt
      841 gcgttcgacc tcgagctcct cgccggcgtc gagcgcgcgc ctgatccctt cccactccgc
      901 aaggttctcc tcggagcgac gcaggtactc gtcgacctcg atccggtacc gctcgatctg
      961 ctcgtcatcg tgcaggaacc acggcacgta ctcggcgccg tgctcggagg actcggtcgg
     1021 gaagtagccg aaccgcttga agagctcgac gcgcacccgc cgcccgagcc cctcggggtc
     1081 ggcatcgacg atcgctcgca gccgcgggta caggtcctcg ccggactcgc ggtcgcggaa
     1141 cacgtagacg aagcactggt ggttgaaccc cgccgttcgg aactcgacgc gccgctccgg
     1201 ggccccgatc gtgcgtgcga ggaacgcgtg cgtgtcgcgg acgctgtggc agacgccgac
     1261 aacgttggcc gcgggcaggc gcgaaccgct ccagatgccc cagggcacca tcgccatcgg
     1321 gttcgtgtag ttgagcagga ggccgtcggg gcatcgttcg accatctcgt cggccatctc
     1381 gatcatgacc gggatcgtgc ggaggcctcg catgatcccg ccgatgccga tcgtgtcgcc
     1441 gatcgtctga cgcagcccga acgtcttcgg gacgtcgaag tcggtccgcg tcgccgggta
     1501 gccaccgacc tgaatctcgt tgatcaggta gtcggcgccg tcgaacgcgg acgcgcgcga
     1561 ctcgtgcgcc gagacctcgt atcccgcgtt cgagcgctcc acgatctgat cggcccccat
     1621 gagcgcgtac gcgagacgtt cggcgtcgat gtcgtggagg gcgatctcga gcgatccgtg
     1681 cagctcctcg tacgcgcaca gatccgcgag gatccccctg gtgaactcga cgctgcccgc
     1741 gccgatgatc gtgaccttcg ccacgatcgc gagtctcgca ccccaccgag cggggcgtcg
     1801 acctcgccgg gtaacgttcc ggccgtgatc gtctgcgtgg cggcgaaccc ctcgatcgac
     1861 aggctgttcg aggtcgaacg gctcgtggcc ggcgggatcc accgtccgct cgggttcgtg
     1921 caggtcgccg gcggcaaggg cctgaacgcg gcgcgcgcgg cccgtcgttt gggcggggac
     1981 gtgcg
//
LOCUS       DATBRK010000039         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2027566, whole genome shotgun sequence.
ACCESSION   DATBRK010000039 DATBRK010000000
VERSION     DATBRK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027566"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VIH70_01195"
     CDS             <1..315
                     /locus_tag="VIH70_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8115008.1"
                     /translation="VTIGVGNAGSRIAPEDREAIFERFRRADGSGAPGAGLGLYISRG
                     LVRAHGGELEVGDVPEGGTRFTFWLPVLDPGEREVRVGPSSGDAIEPNVSHITVPSLG
                     RR"
     gene            354..980
                     /locus_tag="VIH70_01200"
     CDS             354..980
                     /locus_tag="VIH70_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEY8115009.1"
                     /translation="MSEGSIRERMAALSRGELVGLVALLAVTLGGAGLWYVRSLPRPV
                     EVAATPGGGDAPIAAASASPSPEVVILVDVAGWVRRPGVYEFEEGARVIDAIDAAGGA
                     RSGALLESLNLAAPLADGIQILVPRRGETVAPPASGGSVAGVAGLINVNTAAVTELEE
                     LPGVGEVIGQAIVDYRTENGPFTSVDQLLDVSGIGDATLENIRELVTV"
     gene            977..>3027
                     /locus_tag="VIH70_01205"
     CDS             977..>3027
                     /locus_tag="VIH70_01205"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY8115010.1"
                     /translation="MSAWITPALAAVFWAGLLAQPRLGDVVDPWVWLSAGGGLLGAAL
                     ALAPQPAEGTGSLERAGLLERDPRAAVAPATTRYDRPAWLPLGLALIGAVLLAVGWGS
                     LHEHRVRGAVVARLAPAGVTVEGPLRLDPSAERDRWSAIMDVRSVSWDGGAAAVRETV
                     WLSGHDRPPDAVRGDVVRVTGSLRRVEDGGFAEFLLRRGISADLEVDEVARLGPAPFA
                     PVRWAQAFRALAGRSIESSFPPREAGLLMGLALGDDSRLDPVLERDFRATGLSHLLVV
                     SGENVAMVLAPIVALGAALRWSRWPRFALGIGTVAFFVVLTGAEPSVMRAGVMAGLAL
                     IGILLGRPRSTGSILAGAVVVLLAIDPALVWSVGFQLSVAATASMVALATPISQRVRF
                     LPGSLALATGATLAAQVGVTPLLLYYFHEVPVSTVAANVLAFPAVPPALLIGLAAATT
                     GIVFEPLGRLLSVVAQVPMRYLEAVADRLARAPIPWITGGGLPSLVLGLAAAVLIARW
                     LRSGRRLPRKAMVVALGLVPLLLWSSALSAGPTSALTVRFFDVGQGDAALLTSPGGVA
                     ILVDGGPEPDQVATELVALGVKRLDLVIASHPHADHVVGLPAVLARIPAGLLLEPGCE
                     DESPDGAALDEAATSEHIAVRHPRAGDVLWVGDIRLVILSPDRCWSGTESDTNNDAIV
                     VR"
BASE COUNT          314 a         1071 c         1162 g          480 t
ORIGIN      
        1 gtcacgatcg gcgtcggcaa cgccgggagc cggatcgcgc cggaggatcg ggaggcgatc
       61 ttcgagcggt tccgccgagc ggatggatcg ggggcgccgg gcgcgggact cggcctgtac
      121 atctcgcgcg ggctggtgcg cgcacacggt ggcgagctcg aggtgggtga cgtgccggag
      181 ggcgggacgc ggttcacctt ctggctgccc gtgctcgatc cgggcgagcg cgaggtcagg
      241 gtcgggcctt cgagcggcga cgcgatcgaa cccaacgtga gccacatcac ggttccctcg
      301 ctgggtcggc gttagcgttc gggtttcgcg gtctcccccg ggagggggag cccatgtccg
      361 aaggctcgat ccgcgagcgc atggctgctc tctcgcgcgg ggagctggtg ggtctggtcg
      421 cgctcctcgc cgtcaccctc gggggcgcgg gtctgtggta cgtgcggtcg ctccctcgcc
      481 cggtcgaggt ggccgcgact cccggcgggg gcgatgcccc gatcgctgcg gcgtcggcca
      541 gcccgtcgcc ggaggtcgtg atcctcgtgg acgtcgccgg gtgggtgcgc cgtcccggcg
      601 tgtacgagtt cgaggagggc gcgcgggtga tcgacgcgat cgatgcggcg gggggcgccc
      661 gttcgggcgc gctgctggag tcgctgaacc tcgcggcgcc gctggccgat gggatccaga
      721 tcctcgttcc gcgccggggg gagaccgtgg cgccgcccgc gtcgggcgga tcggtcgcgg
      781 gcgtggcggg gctgatcaac gtcaacaccg ccgcggtgac cgagctcgag gagctgcccg
      841 gcgtcggcga ggtgatcggc caggccatcg tcgactaccg gacggagaac gggccgttca
      901 cctccgtcga ccagttgctc gatgtgagcg ggatcgggga cgcaacgctc gagaacatcc
      961 gggagctcgt caccgtgtga gcgcgtggat cacgccggcg ctcgcggccg tgttttgggc
     1021 cggcctgctc gcgcagccgc ggctcggcga tgtcgtcgac ccatgggtct ggctgtcggc
     1081 gggcggcggg ctgctgggag ccgcgttggc tctcgcgccc cagccggcgg agggcacggg
     1141 atcgctggag cgggccgggc tccttgaacg cgatcctcga gcggccgtcg caccggcgac
     1201 cacccgatac gaccggccgg cgtggctccc gctcgggctc gcgctgatcg gagccgttct
     1261 gctcgccgtc gggtggggga gcctgcacga gcatcgggtc cggggcgcgg tcgtcgctcg
     1321 cctcgcgccg gccggcgtca ccgtcgaggg tccgctccgc ctcgatccct cggccgaacg
     1381 cgatcgctgg tcggccatca tggacgtgcg ctcggtcagc tgggacggtg gggcggcggc
     1441 cgttcgagag accgtctggc tctccggaca cgaccggcct cccgacgcgg tccgcggcga
     1501 cgtcgtccgc gtcaccgggt cgctccgacg cgtcgaggac ggaggcttcg cggagttcct
     1561 gctccggcgg ggcatctcgg ccgacctgga ggtcgacgag gtggcgcgtc tggggcccgc
     1621 gccgttcgcg cccgttcgct gggcgcaggc gttccgagcg ctcgccggcc gctcgatcga
     1681 gtcctcgttc ccgccgcgcg aggcggggct gctgatgggg ctcgcgctgg gagacgactc
     1741 gcggctggat cccgtcctcg aacgcgactt ccgcgcgacc gggctctcgc acctgctcgt
     1801 cgtctcgggc gagaacgtcg cgatggtgct cgccccgatc gtggcgctcg gggccgccct
     1861 gcgatggtcc cggtggccgc ggttcgcgct cgggatcggg acggtagcgt tcttcgtcgt
     1921 gctcaccggc gccgagccgt cggtgatgcg cgccggcgtg atggcggggc tcgcgctcat
     1981 cggcatcctg ctcggccggc cgaggagcac gggctcgatc ctcgcgggcg ccgtcgtcgt
     2041 cctgctcgcg atcgatccgg cgctcgtgtg gtcggtaggg ttccagctgt cggtggcggc
     2101 gacggcgagc atggtggcgc tggccacgcc gatctcccaa cgggtccgct tcctgccggg
     2161 gtcgctcgcc ctggcgaccg gagccacgct cgccgcgcag gtcggcgtca ccccgttgct
     2221 cctgtactac ttccacgagg tccccgtctc gaccgtggcc gccaacgtcc tcgcgttccc
     2281 cgcggtgccg cccgcgttgc tgatcggcct ggccgcggcc accaccggga tcgtgttcga
     2341 gccgctcggc cgcctgctct cggtcgttgc ccaggtgccg atgcgctacc tcgaggccgt
     2401 cgcggatcgc ctggcgcgag cgccgatccc gtggatcacc ggcggcggcc tcccgagcct
     2461 ggtcctcggg ctcgcggcgg ccgtcctgat cgcgaggtgg ctgcgctcgg gtcgccgcct
     2521 gccgaggaag gcaatggtgg tcgcgctcgg gctcgtgcca ctgttgctct ggtcaagtgc
     2581 gctctccgcc ggcccgacga gcgcgctcac cgtccgcttc ttcgacgtcg gccaggggga
     2641 tgcggccctg ctcacctcgc cgggcggggt cgcgatcctg gtcgacggcg ggcccgaacc
     2701 cgatcaggtg gccaccgagc tcgtcgcgct cggggtcaag cggctcgacc tggtgatcgc
     2761 gagccacccg cacgccgacc acgtggtcgg gctccccgcc gtgctcgcaa ggatcccggc
     2821 cggcctactg ctcgaacccg ggtgcgaaga cgaatcgccc gacggggcgg cgctcgacga
     2881 ggccgcgacc tccgagcaca tcgcggtccg gcacccgagg gcgggcgacg tcctgtgggt
     2941 cggcgacatc cgcctcgtca tcctgtcgcc cgatcgatgc tggtcgggca ccgagtccga
     3001 caccaacaac gacgcgatcg tcgtgcg
//
LOCUS       DATBRK010000040         8979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_726568, whole genome shotgun sequence.
ACCESSION   DATBRK010000040 DATBRK010000000
VERSION     DATBRK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726568"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /gene="truB"
                     /locus_tag="VIH70_01210"
     CDS             <1..412
                     /gene="truB"
                     /locus_tag="VIH70_01210"
                     /inference="COORDINATES: protein motif:HMM:NF020706.4"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY8115011.1"
                     /translation="IRVDAFAVSAFDGRDLEARVTCSGGTYVRVLVADVGRSLGCGAH
                     LVRLRRTAIGSFPVEDARPPGQGALEPVERAVRHLPGIRLEQEEARAASHGRPLGPAG
                     IVGPYAVFDPEDALVGVYRDEGAMGRPEVILPRR"
     gene            complement(452..1576)
                     /locus_tag="VIH70_01215"
     CDS             complement(452..1576)
                     /locus_tag="VIH70_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY8115012.1"
                     /translation="MSTPRTAERTRKPLGWKRIVGYGISVAIVVGIFWWAIPKFADYR
                     DVWAAITTLTPMETASLVAATFFNLVTYWWANQAALPGLGIGKAAVLTQTTTTVANTL
                     PAGGAIAIGLTYSILDSWGFTGTSVALYVGVTGIWNIFTKLALPMLALVLLAVTGHLT
                     TTYIVAALLGIVVLAVAVGLFALVFRSEAFAVRIGNRMGRVASWVRRRLHKPPVTSWG
                     EGAARFRRDTIVLVEHRWLRLSLMTVLSQLGLFLVLLISLRHLGVSEQEVSGSEAFAV
                     YAFSRLLSAVPITPGGVGLIDLGYIGGLTAFDGAEKAQIVAAVLLFRALTYGIQIPIG
                     GFTYIIWRVKSDWRRPADAAAQRERSTYFALDEGSSRVTR"
     gene            complement(1630..2316)
                     /locus_tag="VIH70_01220"
     CDS             complement(1630..2316)
                     /locus_tag="VIH70_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8115013.1"
                     /translation="MLSNHRRAIGLSVVLLGATVFVFAAVGRHPPELAPTTTLPFVGE
                     FDLSVFHWMDDIRNGVLTLLARFLNVLGGGIVTIPLRIAVTAWLLFRTRWRALATWVI
                     TWAAAELILSGAKTFFHRGRPPLPLVEVVGFSFPSGHAVAGAATAVALVLVVLSPGPA
                     RRRWELAAVAFAFVMAMSRVYLNAHWFSDVVAGVLLGAGVALGSAGVVTEIRDVALRR
                     RSRAAAADHT"
     gene            complement(2318..2908)
                     /locus_tag="VIH70_01225"
     CDS             complement(2318..2908)
                     /locus_tag="VIH70_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115014.1"
                     /translation="MSRRRLAGRVAAIAIALSTLAACSARAGDASEADCPIEDGELFV
                     LMAQSVPTATLIPCIEALPAGWTYGGSDVNDATARFWLDSDRGGLHAVEVSLAASCRI
                     VGAVDVTNSTSEAGVRVYLDEFDLHPFSANKYFVFPGGCVTYRYRFGPDAEPTLALEA
                     DEAVTFGLRTVLVRQVHQELGLTLCGAGAPPCENGD"
     gene            complement(2905..4587)
                     /locus_tag="VIH70_01230"
     CDS             complement(2905..4587)
                     /locus_tag="VIH70_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8115015.1"
                     /translation="MEATTDGIERGGNKGHAHPEQRRRPSGEPPALPHELGRSGKFFL
                     LLVLYFVVAITAFLVFPSLKSVFERWDHTRQLWITDLRTPGLTRVMLVVNGLASSWTI
                     RLIRWGVILALIVFRRWRHLFVFLGAIIVTEIVAYNASSIIARPRPLGIPILTGWQGY
                     SMPSRPMAGLAVSAVGAMYALIVAGRPRSIAKWAIWIVLALLGLARVYLGVDHTTDVV
                     MGAVFGVAVGVASFRLFAPNDLFPVSYQRGKAAHLDVGGRRGEAIVAAIRDQLGLAVL
                     DVKPVGLEGSGGSTPLRIKIEATDDEPERYVFAKLYAKSHVRADRWYKLGRTILYGAL
                     EDEKPFQSVRRFVEYEDYTLRLLNDLGIPVPRPYGIVEITPEREYMIVMEFFEGAVEI
                     GDAEIDDGVIDQGLGMVRRMWDEGLAHRDIKPANLMVRDGELKLIDVFFVQVRPSPWR
                     QAVDLANMMLVLALRTDADRVYAHALGYFSEDEIAEGFAAARGVASPTQLRNNLKQDG
                     RDLLAEFRRIAPEREQVSIQRWSVRRVLLTVGVLFAAWLALGLVVSNWNAFV"
     gene            complement(4602..5528)
                     /locus_tag="VIH70_01235"
     CDS             complement(4602..5528)
                     /locus_tag="VIH70_01235"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8115016.1"
                     /translation="MVGRRSSALAASLALALAACVGQSTPADDARGDDAITVASFAFP
                     ESVLLAEIYAQAIEGAGILVEREPSLGPRELVMPALLQGLVEFVPEYSGSGLQFLAGD
                     GSTSPDHEINNQRFAELLRPLDVTALDASPAEDQNGFAVTAETALRYGLRTLSDLATV
                     SRELVFGGPPECTRRPFCLPGLERSYGITFGQVFSNLDAGGPRTVSALAEGTVDVALL
                     FTSDAAIDLNDFVLLDDDQRLQPAENVTPVIRTGVLDRFGPSLADSVNAVSALLTTGE
                     LRTLNAEVARGATPEQVASGWLASNGLDQGLQ"
     gene            5595..6467
                     /gene="ppk2"
                     /locus_tag="VIH70_01240"
     CDS             5595..6467
                     /gene="ppk2"
                     /locus_tag="VIH70_01240"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY8115017.1"
                     /translation="MATETEGKGGDSHGSKHRGHRDAEDRASKLSKKAYEKELRRLQE
                     ELVKMEDWVSGAGARIVVIFEGRDAAGKGGTIKRITQYTNPRVTRIAALPKPTERERT
                     QWYFQRYIANLPSAGEIVLFDRSWYNRAGVEKVLGFCTPEEHRRFLRQVPIFERLLVE
                     DGIVLLKYWFSVSDEEQERRFRSRIDDPMRRWKLSETDLLSRSHWVDFSRAKDEMFVH
                     TDISEAPWYVVEADDKPRARLNCIAHLLSVIPYEERGAVEIRIPPRQSDEGYVRPARE
                     TYTYVPDHARSLIG"
     gene            6501..7430
                     /locus_tag="VIH70_01245"
     CDS             6501..7430
                     /locus_tag="VIH70_01245"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY8115018.1"
                     /translation="MKRLHGLDALPLDRDRTVVTVGFFDGVHLGHRAVLGRTVEAAGE
                     RGLPSVAVTFDRHPREVLTPGQEPRLLTTVDRKAALIEETGVDTLLVLEFTAEFSRWP
                     AERFVADVLVGGLHVRHCVMGANFTFGHRAAGNVELLIQRGPALGFTAERVELLEVDG
                     RGVSSSSIRDALSRGDLEWPGMALGRRFVLDGTVVSGSGRGHGLGFPTANLRTGPRLL
                     LPGRGIYAGRALVEGVGHVAAISVGTNPTFGSEPLHVEAYLLDFDGDLMGSQIAVEFW
                     AYLRDEERFDTPEQLSAAIGEDVRRTRDLVRDP"
     gene            7506..7775
                     /gene="rpsO"
                     /locus_tag="VIH70_01250"
     CDS             7506..7775
                     /gene="rpsO"
                     /locus_tag="VIH70_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY8115019.1"
                     /translation="MTLTKTLKSQIITEHRRSDADTGSTEVQIAVLSRRIADLTQHLK
                     SHAKDHASRRGLLMLVGRRRRLLEYLRREDIERYREIVAKLGLRR"
     gene            8003..>8979
                     /locus_tag="VIH70_01255"
     CDS             8003..>8979
                     /locus_tag="VIH70_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY8115020.1"
                     /translation="MAITLSREFGNSTLTFESGKLALQAGGSCIVTYGETQVLATCTT
                     AQPREGIDFFPLTIDVEERMYAAGKIPGGFFRREGRPSETAILTARLTDRPMRPSFKD
                     GYRDEVQVVITVLSVDLARQYDIPGMNAASIATMLSGIPFEGPVGSVRLGLIGGEWVI
                     NPTFQDLEEATFDIVVAGRRNDSGQVDILMIEGEAPDNTWALLQGSGTMPTEQVVAEG
                     LEAAKRAIDELIDMQLEFIARADVKPRPFEPKPLYDADTWAAAETFKERLETAIVPDR
                     SEREAAMAEIKDLLKAHLLETWGAESYAARQSEISPAWKDLQKKVMRARV"
BASE COUNT         1479 a         3080 c         3192 g         1228 t
ORIGIN      
        1 gatccgggtc gacgcgttcg cggtgtcggc cttcgacggg cgagatctgg aggcgcgggt
       61 cacgtgctcg ggcgggacgt acgtgcgggt gctcgtcgcc gacgtcggtc ggtccctcgg
      121 ctgcggggcg cacctggtcc gcctgcgtcg gaccgcgatc gggtcgttcc cggtcgagga
      181 cgcccgcccg ccgggccagg gggcgctcga gcccgtggag cgcgccgtcc ggcacctgcc
      241 cggcatccgt ctggagcagg aggaggctcg agcggcgtcg cacggtcgtc cgctcggacc
      301 cgcggggatc gtcggcccct acgcggtgtt cgatccggaa gacgcgctcg tcggcgtcta
      361 ccgggacgag ggagccatgg gcaggcccga ggtgatcctc ccgaggaggt gacgcgcgcc
      421 gcccggccag ggctcacggg tgctcacgac atcacctcgt aacccgcgag gaaccctcgt
      481 ccagcgcgaa gtacgtggac cgctcgcgct gagctgccgc atcggcggga cgacgccagt
      541 ccgacttgac gcgccagatg atgtaggtga atccgccgat cgggatctgg atcccgtagg
      601 tgagcgcccg gaacagcagg acggcggcga cgatctgagc cttctcggcc ccgtcgaacg
      661 cggtgagccc gccgatgtac ccgaggtcga tcaggccgac gccgccgggc gtgatgggaa
      721 ccgccgagag caggcgtgag aacgcataca cggcgaacgc ctcggatccg gacacctcct
      781 gctcggagac accgaggtga cgcaacgaga tcagcagcac gaggaacagc ccgagctggc
      841 tgagcacggt catcaggctc agccggagcc accggtgctc gaccagcacg atcgtgtccc
      901 ggcggaagcg cgccgccccc tcgccccacg aggtgaccgg cggcttgtgg agccgtcggc
      961 gcacccacga ggcgacacga cccatccggt tcccgatgcg gacggcgaac gcctcgctcc
     1021 ggaagacgag ggcgaacagg ccgacggcga ctgccaggac gacgatcccc aagagcgccg
     1081 ccacgatgta ggtggtcgtc aggtgcccgg tgaccgcgag gagcacgagc gccagcatcg
     1141 gcagcgcgag cttggtgaag atgttccaga tgccggtcac acccacgtac agcgcgacgc
     1201 tcgtgccggt gaacccccag gaatccagga tcgagtaggt caacccgatc gcgatggccc
     1261 cgcccgccgg gagcgtgttc gcgaccgtgg tcgtggtctg cgtgagcacg gcggccttcc
     1321 cgatgccgag gccgggcagg gccgcctggt tcgcccacca gtaggtcacg aggttgaaga
     1381 acgtcgcggc cacgagtgac gccgtctcca tcggcgtgag cgtggtgatc gccgcccaca
     1441 cgtcccggta gtcggcgaac ttcgggatcg cccaccagaa gatgccgacc acgatcgcga
     1501 ccgagatgcc gtagccgacg atgcgcttcc atcccagggg cttgcgcgtc cgctcggccg
     1561 tccggggtgt cgacatcacg agaagcctag tccgttgggc gctcgagcat gaggtggagg
     1621 ggctccccgc taggtgtggt ccgcagccgc cgcgcgagat cgacgacgca gcgccacgtc
     1681 gcggatctcg gtgacgacgc cggccgaccc gagcgccacc cccgccccga gcagcacgcc
     1741 cgcgaccacg tccgagaacc agtgggcgtt caggtacacc ctcgacatcg ccatcacgaa
     1801 cgcgaacgcg acggcggcga gctcccaccg tcgtctcgcc gggccgggcg agagcaccac
     1861 gagcaccagg gcgacggcgg tcgcggctcc ggcgacggcg tgcccggagg ggaacgagaa
     1921 cccgaccacc tcgacgagcg ggaggggagg gcgtccgcgg tggaagaacg tcttggcgcc
     1981 gctgaggatc agctcggccg ccgcccacgt gatcacccac gtcgcgagcg ctcgccatcg
     2041 cgtgcggaac aggagccacg cggtcacggc gatccgaagg gggatcgtca cgatgccgcc
     2101 ccccaggacg ttgaggaagc gcgcgaggag cgtcagcacg ccatttcgga tgtcgtccat
     2161 ccagtggaac accgagagat cgaactcgcc gacgaacggg agcgtcgtcg tgggggcgag
     2221 ctcgggggga tgtcgtccca ccgccgcgaa gacgaacacc gtcgcgccga gcaggaccac
     2281 cgacaagccg atcgctcggc ggtgattcga gagcacgcta gtcgccgttc tcgcaggggg
     2341 gtgcgcccgc gccgcacagc gtcagcccga gctcctggtg gacctgacga accaacacgg
     2401 tccgcaggcc gaacgtgacc gcctcgtcgg cctccagcgc gagggtcggc tcggcgtcgg
     2461 gaccgaaccg gtagcggtag gtcacacagc cgccggggaa gacgaagtac ttgttcgccg
     2521 agaaggggtg caggtcgaac tcgtcgaggt agacgcggac gccggcctcg ctagtggagt
     2581 tcgtgacgtc gacggccccg acgatccggc aagaggcggc gagcgagacc tcgacggcgt
     2641 gcaggccacc gcggtccgag tcgagccaga accgcgccgt ggcgtcgttc acgtccgagc
     2701 ctccgtacgt ccaccccgca ggcagggctt cgatgcaggg gatgagggtg gcggtcggga
     2761 cggactgagc catcaggacg aacagctcgc cgtcctcgat cgggcagtcg gcctccgagg
     2821 cgtcgccggc gcgcgcgctg caggccgcga gggtggacag ggcgatcgcg atcgcggcga
     2881 ccctgccggc gagccgtcgc cgactcatac gaacgcgttc cagttgctga cgaccaggcc
     2941 cagcgcgagc caggccgcga acagcacgcc gaccgtgagc agcacccggc gcacgctcca
     3001 tcgctggatc gagacctgtt cgcgctccgg cgcgatccgt cggaactcgg cgaggaggtc
     3061 gcggccgtcc tgcttgaggt tgttgcggag ctgcgtcggg ctcgcgacgc cgcgcgccgc
     3121 cgcgaacccc tcggcgatct cgtcctcgga gaagtagccg agcgcgtgcg cgtagacacg
     3181 atcggcgtcg gtgcggagcg cgaggacgag catcatgttg gcgaggtcga ccgcctgccg
     3241 ccacggggag ggtcgcacct ggacgaagaa cacgtcgatc agcttcagct cgccgtcgcg
     3301 caccatcagg ttcgcgggct tgatgtcgcg gtgcgcgagc ccctcgtccc acatcctgcg
     3361 aaccatgccg agcccctggt cgatcacgcc gtcgtcgatc tccgcgtccc cgatctccac
     3421 cgcgccctcg aagaactcca tgacgatcat gtactcgcgc tcgggcgtga tctcgacgat
     3481 cccgtacggc cgggggaccg ggatcccgag atcgttcagc aggcggagcg tgtagtcctc
     3541 gtactcgacg aaccggcgca cggactggaa cggcttctcg tcctcgagag cgccgtacag
     3601 gatcgtgcgg ccgagcttgt accagcggtc ggcccgcacg tgactcttcg cgtagagctt
     3661 ggcgaagacg tagcgctcgg gctcgtcgtc cgtcgcctcg atcttgatcc gcagcggggt
     3721 cgagcctccg gacccctcca ggcccacggg cttgacgtcc aggaccgcca gtcccagctg
     3781 atcgcggatc gccgcgacga tcgcctcgcc gcgacggccg ccgacgtcca ggtgagccgc
     3841 cttcccccgc tggtaggaca cggggaacag atcgttgggc gcgaacaggc ggaagctcgc
     3901 gacgccgacc gccacgccga acacggcccc catcaccacg tccgtggtgt ggtccacgcc
     3961 gaggtacacg cgggcgagcc cgagcaatgc caggacgatc cagatcgccc acttcgcgat
     4021 cgagcggggc ctgcccgcca cgatcagggc gtacatggcc ccgacggccg agaccgccag
     4081 accggccatc ggcctcgacg gcatcgagta cccctgccac ccggtgagga tcgggatccc
     4141 caggggccga gggcgggcga tgatcgacga ggcgttgtag gcgacgatct cggtgacgat
     4201 gatcgcgccg aggaacacga acaggtggcg ccaccggcgg aacacgatca gcgcgaggat
     4261 cacgccccac cggatcaggc ggatcgtcca gctcgatgcc agcccgttga cgacgagcat
     4321 cacgcgggtg agcccgggcg tccgcagatc cgtgatccag agctgccggg tgtggtccca
     4381 gcgctcgaag acgcttttca gtgaggggaa caccaggaag gcggtgatgg cgacgacgaa
     4441 gtacaggacg agcaacagga agaacttccc ggagcggccg agctcgtgcg gaagcgcggg
     4501 cggctctccg gagggacggc ggcgctgctc ggggtgcgcg tggcccttgt tcccgccccg
     4561 ctcgatccca tcggtcgtcg cttccatgcc gttcggcgac tctactgcag cccttgatcg
     4621 agaccgttcg acgcgagcca gccagacgcg acctgctcgg gggtcgcacc gcgcgcgacc
     4681 tccgcgttca gcgttcggag ctcgccggtc gtcaggagcg ccgagaccgc gttcacgctg
     4741 tcggccagcg acggcccgaa gcggtcgagc acgccggtcc ggatgaccgg cgtgacgttc
     4801 tcggcgggct ggaggcgctg gtcgtcatcg agcaggacga agtcgttcag gtcgatcgcg
     4861 gcgtcgctcg tgaacaggag cgccacgtcc accgtcccct ccgcgagggc ggagaccgtg
     4921 cgcggaccgc ccgcgtcgag gttgctgaag acctgcccga acgtgatccc gtacgaccgc
     4981 tccagaccgg gaaggcagaa gggtcgccgg gtgcattcgg gcggtccacc gaagaccagc
     5041 tcgcgggaga ccgtcgcgag gtcgctgagc gtccggaggc cgtaccgcag cgcggtctcc
     5101 gccgtcaccg cgaacccgtt ctgatcctcg gcgggtgatg catcgagcgc ggtcacgtcg
     5161 agcggtcgca gcagttcggc gaaacgctgg ttgttgatct cgtgatccgg cgaggtcgat
     5221 ccgtcgcccg ccaggaactg gagcccgctg ccggagtact ccgggacgaa ctccacgaga
     5281 ccctggagca gcgcgggcat cacgagctcg cgcggcccca gcgagggctc gcgctcgacc
     5341 aggatgccgg caccttcgat cgcctgcgcg tagatctcgg ccagcaggac gctctcgggg
     5401 aaggcgaacg acgccacggt gatcgcgtcg tcgccgcgag cgtcgtccgc gggcgtgctc
     5461 tgcccgacgc atgccgccag cgcgagggcc agcgacgccg cgagcgcgga cgaccggcgg
     5521 ccaaccatga cggcccaagc ctaggccaag cccggcgcgc ccacgtggga tgatgcctgg
     5581 tccggagggg cgcgatggcg acggagaccg agggcaaggg cggagacagt cacggatcca
     5641 agcaccgtgg gcatcgcgac gccgaggatc gcgcgagcaa gctgtcgaag aaggcctacg
     5701 agaaggagct ccggcgcctc caggaagagc tcgtgaagat ggaggactgg gtctcgggcg
     5761 cgggggccag gatcgtggtc atcttcgagg gtcgggacgc ggccggcaag ggcggcacga
     5821 tcaagcggat cacgcagtac acgaacccgc gcgtgactcg catcgcggcg ctccccaagc
     5881 ccaccgagcg cgaacgcacc caatggtatt tccagcggta catcgcgaac ctgccctcgg
     5941 ccggcgagat cgtgctgttc gaccgcagct ggtacaaccg cgcgggcgtc gagaaggtcc
     6001 tggggttctg cacgcccgag gagcaccgac ggttcctgcg gcaggtcccg atcttcgagc
     6061 ggctcttggt cgaggacggc atcgtcctgt tgaagtactg gttctcggta tcggacgagg
     6121 agcaggagcg ccgcttccga tcgcggatcg acgacccgat gcgccgctgg aagttgtcgg
     6181 agacggacct cctctcccgc tcgcactggg tcgacttctc gcgcgcgaag gacgagatgt
     6241 tcgtgcacac ggacatctcg gaggcgccat ggtatgtcgt ggaggccgac gacaagccgc
     6301 gggcgcgact gaactgcatc gcgcacctgc tctcggtgat cccgtacgag gagcgtggtg
     6361 cggtcgagat caggatcccg ccgcgtcaga gcgacgaggg ctacgtgcga cccgcgcgtg
     6421 agacctacac ctacgtgccg gatcacgcac gctcgctcat cggctaggcc cgttcggacg
     6481 tccgttaccc tcgggtgccg atgaagcgac tccacgggct cgacgccctt ccgctggacc
     6541 gggatcgcac ggtcgtcacg gtcggcttct tcgacggcgt ccacctcggt caccgcgcgg
     6601 tcctcggtcg cacggtcgag gccgctggcg agcgagggct cccttcggtt gccgtgacct
     6661 tcgatcgcca tccgcgcgag gtgctcacgc ccggccagga gcctcgcctg ctgacgacgg
     6721 tggaccggaa ggccgcgctg atcgaggaga ccggggtcga cacgctcctg gtgctcgagt
     6781 tcaccgcgga gttcagccgg tggccggccg agcgattcgt ggcggacgtg ctcgtcggcg
     6841 ggctccacgt ccgccactgc gtgatgggcg cgaacttcac gttcggtcac agggccgccg
     6901 ggaacgtcga gctgctcatc cagcgcggac cggccctcgg gttcaccgcg gaacgcgtcg
     6961 agctcctgga ggtcgacggg cgcggggtgt cgtcgtcctc gatccgcgac gcgctgtccc
     7021 gcggcgacct cgagtggccc ggcatggcgc tgggacgccg gttcgtcctg gacggcaccg
     7081 tcgtgagcgg ctcgggccgg ggccacggcc tcggcttccc gaccgcgaac cttcggaccg
     7141 ggccgcgcct gctgctcccg ggccgcggga tctacgcggg ccgggctctc gtggagggcg
     7201 tgggccacgt cgcggcgatc agcgtcggga ccaacccgac gttcgggagc gaacccctgc
     7261 acgtggaggc gtacctgctg gacttcgacg gcgacctgat ggggagccag atcgcggtcg
     7321 agttctgggc gtacctgcgc gacgaggagc ggttcgacac gcccgaacag ctgtcggccg
     7381 ccatcgggga ggacgtccgg cggacccgtg acctcgttcg cgacccgtga gcggccgggc
     7441 gtgagccgct gaccagcgca gacgggcggg gcgcgccgga gcgtctggta gcctggtcga
     7501 gaccgatgac gctcaccaag accctgaagt cccagatcat cacggagcat cggcgttccg
     7561 acgcggacac cggttcgacc gaggtgcaga tcgccgtgct cagcaggcgg atcgccgacc
     7621 tcacccagca cctgaagtcc cacgccaagg accacgcgtc ccggcgcggc ctcctgatgc
     7681 tcgtgggccg gcggcgtcgt ctcctcgagt acctgcgacg agaggacatc gagcggtatc
     7741 gagagatcgt ggcgaagctc ggacttcgtc gatagacccg gaggcgcccc ggtggggcgc
     7801 cttctccgta ggagggatgc gaacggatca cccatcggaa ccggaggcgg gcttccccgc
     7861 ggtcggttct cagtagcgag cctccgatca ccaggatcgg cggttcacca ctgacaaccg
     7921 gcccgacccg acctcccagc cgcgcggcgc ggtcgcgccg cgcatccaga cacgagccaa
     7981 ggaaacgagg aggcatcggc ccatggccat caccctttcc cgagagttcg ggaacagcac
     8041 gttgacgttc gaatcgggca agctcgccct gcaggcgggc ggttcgtgca tcgtgacgta
     8101 cggcgagacg caggtcctcg ccacctgcac caccgcgcag ccccgcgagg ggatcgactt
     8161 cttcccgctc acgatcgacg tcgaggagcg tatgtacgcc gccggcaaga tccccggcgg
     8221 gttcttccgc cgcgagggtc gcccgtccga gacggcgatc ctgacggcgc gcctcaccga
     8281 ccgccccatg cggccgagct tcaaggacgg ctaccgcgac gaggtccagg tcgtgatcac
     8341 cgtgctctcg gtcgacctgg cgcgtcagta cgacatcccc ggcatgaacg cggcgagcat
     8401 cgcgacgatg ctgtcgggga tcccgttcga gggcccggtc ggctccgtcc ggctggggct
     8461 gatcggcggc gagtgggtca tcaaccccac gttccaggac ctggaggagg ccacgttcga
     8521 catcgtggtg gccggccgcc gcaacgattc ggggcaggtc gacatcctga tgatcgaggg
     8581 cgaggcgccc gacaacacgt gggcgctcct gcaagggagc ggcacgatgc cgaccgagca
     8641 ggtcgtcgcc gagggcctgg aggcggccaa gcgcgcgatc gacgagttga tcgatatgca
     8701 gctcgagttc atcgcaaggg ccgacgtcaa gcccaggccg ttcgagccca agccgctcta
     8761 cgacgcggac acctgggcgg ccgccgagac gttcaaggag cggctcgaga ccgcgatcgt
     8821 gcccgaccgt tcggagcgcg aggccgcgat ggccgagatc aaggacctcc tgaaggcgca
     8881 cctgctcgag acctggggcg cggagtccta cgccgcgcgc cagtccgaga tctcgcccgc
     8941 gtggaaggac ctgcagaaga aggtcatgcg ggcacgcgt
//
LOCUS       DATBRK010000041        10868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_437547, whole genome shotgun sequence.
ACCESSION   DATBRK010000041 DATBRK010000000
VERSION     DATBRK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10868
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437547"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VIH70_01260"
     CDS             complement(<1..1505)
                     /locus_tag="VIH70_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY8115021.1"
                     /translation="MTDQRPWFATYPPGVPHSLEPYPSLSVFGMLEASARTHPEAPAL
                     AWFGRKISYAALLREVERCSAALAGLGVGRGDRVALIMPNCPQYVIAFYATARLGAVV
                     VGNNPLYTAREMEHQLRDCAPSVVVVLDLLYSDFADVIETVGLRHVVVARLNDYMSFP
                     KKQLAPPLKFKKLQREQGKPWPPVPRDAPVTWWERWLDVAGQVPVAATIEPETDPAGF
                     IYTGGTTGISKGAMLSHRNMVSNAMQAAAYLSLVEGEEALLGPLPFFHSFGMLTMNVA
                     VLIAGKMVPIPNPRDLHLVLEEMSKEKPTFVPGVPRLFDALNESPLAAKFDLRSVKAC
                     ISGAAPLPAAVAERFAEITGGARLVEGYGLTECSPVTHANPLDAPRAGSIGLPMPDTD
                     CKIVDLEDPDRSLEPGERGELCIRGPQVMLGYWNRPEETALAIRNGWFHTGDVAVMDP
                     DGYFRIVDRLKEMILVSGFNVYPNEIEDAMYRHPKISKVAVIGVPDDKTGEA"
     gene            1615..3906
                     /locus_tag="VIH70_01265"
     CDS             1615..3906
                     /locus_tag="VIH70_01265"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY8115022.1"
                     /translation="MADPLDEFLDAARSALPPVEGELSVAGLREPVEVLRDVWGIPYL
                     SAASLEDLWFAQGFVQGSERLFQIELALRAAGGRLSGWFSDLTIASDRFARTVGFHRI
                     GVREARRWNAASRSMMRRFVDGARAWMEAMPAPPLEYAMLGVTPELPEDLEGWAAAFA
                     YLAWGLSGNWDHELLRLRLSERFGAETAAALLPPPAVDRAGLAAGSLAGRLLDGLPRS
                     RGQGSNSWVVSGSRTASGRPLLANDPHLLVQQPPAWFELHLRAPGYEARGVALPFVPG
                     ILVGTTPHHAWGLTNVSGDTQDLYVERLNDEGTAAEFDGTWEPLTVHVETIEVRGGAA
                     VAFEVRETRHGPILETAPVGVAGNEYARVDGTFALRWTAADGLLEPVVLAELVRAASF
                     DEFREALRGLWCPGQNVVYADLDGTIGYQCTGRFPLRRAGDGSAPVAGWTSEHEWDGY
                     LPYEELPWSRDPGSGFVVTANNRIHDEDYPHLIGLDFHTGFRARRISELLEPNRAQTP
                     DDAASIQVDTVSLPAQRLLPHLLATEAPGDAVRRALGLLDGWSGDLDAGSAAAAVYEV
                     WVARIAVVAFGDAGDGSPVDAYVAWREMFVCSALPAMLDANIPPPGGGSWDEVLAVAL
                     EEAVALLAERFGSDERGWRWGALHRVRFAHPLARLPGIGALFVAAEHELGGDEQTVLQ
                     GGFDARLGFDAVVVPSWRVVVDLGDLDASLAVLTTGQSGNPASPHWNDQSHLWASGRL
                     RACPFSRPAVEAAAEHTLLLVPR"
     gene            3920..4168
                     /locus_tag="VIH70_01270"
     CDS             3920..4168
                     /locus_tag="VIH70_01270"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEY8115023.1"
                     /translation="MPKSRQRPKKTSRSYVAPPTKPKPKPSPRWYGFFVLGLILLGVL
                     IIVLNYADFLLPGGTQQHWLWIGLGVIGAGFVAATRWR"
     gene            complement(4338..4601)
                     /locus_tag="VIH70_01275"
     CDS             complement(4338..4601)
                     /locus_tag="VIH70_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115024.1"
                     /translation="MSVWIVLSAIAVAVLIAWLGLTALRAASGVGLDLESPEPEDVDD
                     LEVYLVCRECGTEFRVTRLGELQIPRHCGERMEVVRRPAGAGA"
     gene            4731..6092
                     /gene="gdhA"
                     /locus_tag="VIH70_01280"
     CDS             4731..6092
                     /gene="gdhA"
                     /locus_tag="VIH70_01280"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEY8115025.1"
                     /translation="MSAYVDRLMADVEAKNPGELEFHQAVREVATSIELLLDRKPFYR
                     DHKMLERMIEPERVLMFRVPWADDRGEIHVNRGFRVEMSSAIGPYKGGLRFHPTVYLG
                     LLKFLAFEQVFKNALTTLPMGGGKGGADFDPKGKSDAEVMRFCQSFMTELQRHIGPNT
                     DVPAGDIGVGAREIGYLFGQYKRIKNEFTGVLTGKGLEWGGSLIRPEATGYGAVYFAH
                     EMLLTRDDSLEAKTCLVSGSGNVAQYTIEKLLDMGAKAVTLSDSTGFVHDPAGIDNEK
                     LTWVMELKNERRGRIAEYAERFPGATFTPLDPTADANPLWSVPADCAFPSATQNEINA
                     KDAANLLAGGVYVVSEGANMPTSPEGVDQFVNAGILYGPGKAANAGGVAVSGLEMAQD
                     SLRMAWPREEVDARLKDIMKSIHKQCRQTAEEYGTPGNYVNGANIAGFVKVADAMLDQ
                     GLV"
     gene            6176..6679
                     /locus_tag="VIH70_01285"
     CDS             6176..6679
                     /locus_tag="VIH70_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY8115026.1"
                     /translation="MGRERRATSERGMDALYRAHAAEALRLGYLLTGDRGLAEDLVQD
                     AFVRVLGRFHDMRNRDAFWWYLRRTIVNLSTSYFRRRGVERAWLARQRPDEAAPAPHD
                     LAERDRLRTALMTLRPEQRAAIVLRFYEDLSEADTAEALGMPLGTVKSAVSRGLERLR
                     HELPEEA"
     gene            6679..7965
                     /locus_tag="VIH70_01290"
     CDS             6679..7965
                     /locus_tag="VIH70_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115027.1"
                     /translation="MDTRLRTLLHDLASEMPVDIEGTAPKTLRRARGRRVLTAGAGAA
                     IAIALVVVGVSAMPFGNEATLTPAVTGPNPSATFQGLWPETNADALAETQAAVDEGHY
                     PLQASPEGIASLLAVNLLGWEPGDDRAERVDVRGSEAEVVIGNRTFADSVAPVNIETR
                     QLGRTGPRGVWSVVDVSTPLIRLDPVAEIAPGVIGISGGVTHRYDGAAWMDANVFDGP
                     KPEPSLGSARYELTDRTFAFEIHVSPTPDGRATLLLTMPDAVGASLGAVMVPVPTPVG
                     EPEPLEGPNLEGVPPDVAVTAQRIYDAALAGDVDALAPLLDPNTFAYNFDDGSDPIPA
                     WRADPSVLDPMVAVLELPAAEPRTIEGYGTLTIWPYLIDSDFDALTEREADDLQALGY
                     STADIQLMIDGGNGYQGPRLAIDETGLWRSFTTMGE"
     gene            complement(7972..9774)
                     /locus_tag="VIH70_01295"
     CDS             complement(7972..9774)
                     /locus_tag="VIH70_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8115028.1"
                     /translation="MPAQPLWERRFRAPTRTLPAWGPAAPDRFVLLSDEQGSFQAYAW
                     EPGSDPVRRSDEPVGVSLATISADGSRVVWFSDPTGDESGRWLAVPFEGGDPVELLPD
                     APVGWPEGLSLGRELAVAVLADRDGFAVYVSEDGAAAKEIYRHPDLIGISDSELHLEG
                     FDHVGLSADETLVCLEVAQDGDNIHRKLLVLDPRTGAVVGELADGPDLGVRAFAWSPV
                     PGDQRILIGDEREDLLRPGVWAPTTGERKDLPLDLPGEAVPVDWWPDAGSVLIAHLFR
                     GRDRLYRVDLGRGSLSEIDHPRGEILGARVRPDGRVWLRLSRGHRAPVLLDDTGAEVL
                     PRAPGGVRTGRPYREWLFRNPARDVVQGWLVTPPGDGPFPLYVKVHGGPDWLYLDTWF
                     PDVQSLVDEGFAVAMVNYRGSIGFGRRWRDHIIGNIGFPEVEDVVAGRADLVARGIVD
                     PERVVIGGWSWGGYITLLALGLHRDLFAAGVAGVPVGDYLDSYDDSAPSLQAYDRTLA
                     GGVVHDIPEFIAERSPITYVKDVRAPVFVLVGEHDTRCVPSQVYRYVRALKKAGGDVE
                     LYSYGEGHSSYIVDEQVREWGAVLEFLRRRVRLP"
     gene            complement(9827..10261)
                     /locus_tag="VIH70_01300"
     CDS             complement(9827..10261)
                     /locus_tag="VIH70_01300"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEY8115029.1"
                     /translation="MRLAGVLVLGFLLAGCTAERVTEPLTSSPPTPTRSSAAVRTAVE
                     DPAIAIETPFVDGEVGSPVSVTGTADVVDAALTVRVLGEDGTELAAIVVRASCGDGCR
                     GTFATKLFYFIERSQPGTIQVYGIGEDGERALASVPVVLFPP"
     gene            10326..>10868
                     /locus_tag="VIH70_01305"
     CDS             10326..>10868
                     /locus_tag="VIH70_01305"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8115030.1"
                     /translation="MRTRIAVIVGTCLAAQLVVASTTTAAGARIRAREVVGGLNQPVA
                     FTFGPGRKVWYVEKETGQVRIHDLDTDADRLFVTVGGVNGDGERGMLGIALHPSYPAK
                     PLVYVYATRSAGGRLSNQILRYRDRHGSGVNRTVLFTSVAGGSPYHNGGRIAFGPDGM
                     LYAIVGDAHDSSNAQDTTNED"
BASE COUNT         1626 a         3833 c         3880 g         1529 t
ORIGIN      
        1 gcctccccgg tcttgtcgtc cggcaccccg atcaccgcga ccttggagat cttcgggtgc
       61 cggtacatgg cgtcctcgat ctcgttcggg tacacgttga acccggagac caggatcatc
      121 tccttgagcc ggtcgacgat gcggaagtac ccgtcgggat ccatgaccgc gacgtcgccg
      181 gtgtggaacc agccgttgcg gatcgcgagc gcggtctcct cgggccggtt ccagtacccg
      241 agcatcacct gcggcccgcg gatgcagagc tcgccgcgct cgccgggctc gagcgatcgg
      301 tccggatcct ccaggtcgac gatcttgcag tccgtgtcgg gcatcggcag gccgatcgaa
      361 cccgcccgcg gcgcgtccaa cgggttggcg tgcgtcaccg gcgagcattc ggtgagcccg
      421 tagccctcga cgagcctggc gccgcccgtg atctcggcga accgttccgc gaccgcggcc
      481 ggcagcggcg ccgcgcccga gatgcacgcc ttgaccgaac gaagatcgaa cttcgcggcg
      541 agcggcgact cgttcagcgc gtcgaacagg cgcggaacgc ccggcacgaa ggtcggcttc
      601 tccttggaca tctcctcgag cacgagatgc aggtcacgcg ggttcgggat cgggaccatc
      661 ttgcccgcga tcagcaccgc gacgttcatc gtcagcatcc cgaacgagtg gaagaacggg
      721 agcgggccga ggagcgcctc ctcgccctcc accaaagaca ggtacgcggc ggcctgcatc
      781 gcgttcgaca ccatgttgcg atgcgagagc atcgcgcctt tggagatccc ggtcgtgccg
      841 ccggtgtaga tgaagcctgc cggatcggtc tcgggctcga tcgtcgccgc gaccggcacc
      901 tgcccggcga cgtcgagcca tcgttcccac catgttaccg gggcgtccct cggcaccggg
      961 ggccagggct tgccctgctc ccgctgcaac ttcttgaact tcagcggggg cgcgagctgc
     1021 ttcttcggga acgacatgta gtcgttcagc cgcgcgacga cgacgtgccg cagccccacg
     1081 gtctcgatca cgtccgcgaa gtccgagtac agcaggtcga gcacgacgac gaccgacggc
     1141 gcgcagtcgc ggagctggtg ctccatctcg cgcgccgtgt agagcgggtt gttgccgacg
     1201 accaccgcgc caaggcgggc cgtcgcgtag aacgcgatca cgtactgggg gcagttcggc
     1261 atgatgagcg ccacccgatc gcccctgccg acccccagcc cggcgagcgc cgccgagcag
     1321 cgttcgacct cgcgcaggag cgccgcgtag gagatcttcc ggccgaacca cgcgagcgcc
     1381 ggcgcctccg gatgcgttcg cgccgacgcc tcgagcatcc cgaacacgga caggctcggg
     1441 tacggctcca gggagtgggg gacgccgggc ggatacgtcg cgaaccaggg ccgctggtcg
     1501 gtcatcgcag gcatcattcc agacgggcga gcacccgctc aaggtcgcac ctcggctcgc
     1561 tgctcggcgc gctcctcgtc gagcgtccac ggtcagcggc taggctcccg ccgcatggcc
     1621 gatccactcg acgagttcct ggacgcggca cgctctgccc tcccgccggt ggagggcgag
     1681 ctgtcggtcg cggggctccg cgaaccggtc gaggtcctgc gggacgtgtg ggggatcccc
     1741 tacctgtccg ccgcctcgct ggaggacctg tggttcgcgc aggggttcgt gcaggggtcg
     1801 gagcggctgt tccagatcga gctcgcgctg cgcgcggcgg gcgggcgcct ctcgggatgg
     1861 ttctcggacc tcacgatcgc gtccgaccgg ttcgcgcgga cggtggggtt ccaccggatc
     1921 ggggttcgcg aggctcggcg ctggaacgct gcatctcggt cgatgatgcg gcggttcgtc
     1981 gacggggccc gtgcgtggat ggaggcgatg ccggccccgc cgctcgagta cgcgatgctc
     2041 ggggtgaccc cggagctccc cgaggatctc gagggttggg ccgcggcgtt cgcctacctc
     2101 gcgtggggcc tctcgggcaa ctgggaccac gagctgctcc ggctccgcct ctccgagcgg
     2161 ttcggagccg agaccgcggc ggccctcctg ccgccgccgg cggtcgaccg ggccgggctc
     2221 gcggcgggct ccctcgcggg gcggttgttg gacgggctcc cacgttcgcg cggccagggc
     2281 tcgaacagct gggtcgtctc gggttcgagg acggcgagcg gacgcccgct gctggccaac
     2341 gacccgcacc tgctcgtgca gcaaccgccg gcctggttcg agctccacct gcgggcgccc
     2401 gggtacgagg cccgcggggt ggcgctgccg ttcgtcccag ggatcctggt ggggacgacg
     2461 ccgcaccacg cgtggggcct gaccaacgtg agcggcgaca cccaggatct ctacgtcgag
     2521 cgcctgaacg acgaggggac cgccgccgag ttcgacggca cctgggagcc gctcaccgtg
     2581 catgtggaga cgatcgaggt ccggggcggc gccgcggtcg cgttcgaggt gcgggagacc
     2641 cggcacggcc cgatcctgga gaccgcgccg gtcggtgtgg ccggcaacga gtacgcgcgc
     2701 gtggacggca ccttcgcgct gcgatggacc gccgccgacg gtctcctcga gcccgtcgtg
     2761 ctcgccgagc tcgtgcgcgc ggcgtcgttc gatgagttcc gcgaggcgtt gcgcggcctg
     2821 tggtgccccg gccagaacgt cgtgtacgcc gacctcgacg gcacgatcgg ctaccagtgc
     2881 acggggcggt tcccgctgcg gcgcgccggg gacggctccg ctccggtcgc gggctggacc
     2941 tccgagcacg agtgggatgg ctacctcccg tacgaggagc tcccgtggtc gagggacccg
     3001 ggctcggggt tcgtcgtcac cgcgaacaac cgcatccacg acgaggacta cccgcacctg
     3061 atcggcctcg acttccacac ggggttccgc gcgcggcgga tctccgagct gctggagccg
     3121 aaccgggcgc agacacccga cgacgccgcc tcgatccagg tcgacaccgt ctcgctgccg
     3181 gcacagcggc tgctgccgca cctgctcgcg accgaggcgc caggcgacgc ggttcgacgt
     3241 gcgctcggcc tgctcgacgg atggtccggc gacctggacg cgggctcggc ggccgcggcg
     3301 gtctacgagg tgtgggtcgc acggatcgcc gtcgtcgcgt tcggggacgc gggcgatggg
     3361 agcccggtcg acgcctacgt cgcctggcgc gagatgttcg tctgctccgc gctcccggcg
     3421 atgctggacg cgaacatccc gccgcccggc ggtggatcgt gggacgaggt cctcgccgtc
     3481 gcgctggagg aggcggtcgc gctgctcgcc gaacggttcg ggagcgacga acgcgggtgg
     3541 cggtggggcg cgttgcaccg ggtccggttc gcgcacccgc tcgcccgcct gccggggatc
     3601 ggcgccctgt tcgtcgcggc cgagcacgag ctgggcgggg acgagcagac cgtcctgcag
     3661 ggcgggttcg acgcgcggct ggggttcgac gccgtggtgg tcccctcctg gcgggtggtg
     3721 gtggatctcg gcgacctcga cgcgtcgctc gccgtgctca ccaccggaca gtccggcaac
     3781 ccggcgtctc cgcactggaa cgaccagtcg cacctgtggg cctcggggag gctccgggcg
     3841 tgccccttct cgcggcccgc cgtcgaggcg gccgccgagc acaccctgct gctcgtcccc
     3901 cggtaacatc gtcggcgcga tgccgaaatc ccgacagcga ccgaagaaga cctcgcgctc
     3961 gtacgtcgcg ccgccgacga agccgaagcc caagccgagt ccccgctggt acggcttctt
     4021 cgtcctgggt ctgatcctgc tgggcgtact gatcatcgtc ctgaactacg ccgacttcct
     4081 cctgcccggc ggaacgcagc agcactggct gtggatcggc ctcggcgtga tcggcgcggg
     4141 cttcgtcgcc gccacgcgct ggcgctgatc ccttcccgat cagaacgaga tcacatccgt
     4201 gtgattcccc acagctccgt ccacaggtgt gcaaggatgc gctcctctcg tcgggtgtgg
     4261 acaaccccgg gagatctggg gagaacaccg ggagaacccg cacgcagccg ggtgctcggt
     4321 gtggacaacc gatgggacta cgcccccgcg ccggcgggtc tgcgcacgac ctccatccgc
     4381 tcgccgcagt gccgtgggat ctggagctcg ccgagccggg tcacccggaa ctcggtcccg
     4441 cactcgcgac acacgaggta gacctccagg tcatcgacgt cctcgggctc gggggactcg
     4501 aggtccaggc caacccccga ggcggcccgg agcgcggtca ggccgagcca ggcgatcagc
     4561 acggcgaccg cgatcgcgct cagcacgatc cacacgctca tcgagggaag tctacgcacg
     4621 gccgagtgga tgacttggtc ggccggacgt tccgagcacc ccgggccgcc tgggcccggc
     4681 tcggctaggc ttgcgggccg agaccgaacc cgccgggagg cacggggacc gtgagcgcgt
     4741 acgtcgatcg gctgatggcg gacgtcgagg cgaagaaccc cggcgagctc gagttccatc
     4801 aggcggtccg ggaggtcgcc acctcgatcg agctcctcct cgatcgcaag cccttctacc
     4861 gggaccacaa gatgctcgag cggatgatcg agcccgaacg cgtgctgatg ttccgcgtcc
     4921 cgtgggccga cgaccgcggc gagatccacg tgaaccgggg gttccgcgtc gagatgagta
     4981 gcgcgatcgg cccgtacaag ggcggcctgc ggttccaccc gaccgtctat ctcggccttc
     5041 tgaagttcct cgcgttcgag caggtcttca agaacgccct caccacgctt ccgatgggag
     5101 ggggcaaggg cggcgcggac ttcgacccca agggcaagag cgacgccgag gtgatgcgct
     5161 tctgccagtc gttcatgacc gagctccagc gccacatcgg cccgaacacg gacgtgccgg
     5221 ccggcgacat cggcgtgggc gcgcgggaga tcggctacct gttcgggcag tacaagcgga
     5281 tcaagaacga gttcaccggg gtgctgaccg ggaagggcct cgagtggggc ggctcgctga
     5341 tccgtcccga ggcgacgggc tacggcgcgg tctacttcgc gcacgagatg ctcctcaccc
     5401 gcgatgacag cctcgaggcc aagacctgcc tggtctcggg cagcggcaac gtggcccagt
     5461 acacgatcga gaagctgctc gacatgggcg cgaaggccgt cacgctctcg gactccaccg
     5521 ggttcgtgca cgatccggcc gggatcgaca acgagaagct cacctgggtc atggagctga
     5581 agaacgagcg ccgcggccgg atcgccgagt acgccgagcg ctttcccgga gccaccttca
     5641 cgcccctcga tccgaccgcc gatgccaacc ccctgtggag cgtgccggcg gactgcgcgt
     5701 tcccgagcgc gacgcagaac gagatcaacg cgaaggacgc ggctaacctt ctggccgggg
     5761 gcgtctacgt cgtgagcgag ggcgccaaca tgcccacctc gcccgagggc gtcgaccagt
     5821 tcgtgaacgc cgggatcctg tacggcccgg gcaaggccgc gaacgccggc ggcgtggccg
     5881 tctccggtct ggagatggcg caggactcgc tccggatggc gtggccccgc gaggaggtcg
     5941 acgcgcggct gaaggacatc atgaagtcca tccacaagca gtgccgccag accgcggagg
     6001 agtacgggac gcccgggaac tacgtgaacg gcgcgaacat cgccgggttc gtcaaggtcg
     6061 ccgacgcgat gctcgaccag gggctggtct aaccgatcgt cctggtcgac cggatcccgt
     6121 cgcgcaggaa ccccggacgg cacctccgcg tcttcgagct gtgacggtct tgacggtggg
     6181 tcgcgagcgg cgggcgacga gcgagcgggg catggacgcc ctgtatcgcg cgcacgcggc
     6241 cgaggccctg cgcctcggct acctgctgac cggggaccgc ggcctcgccg aggacctggt
     6301 gcaggatgcg ttcgtcaggg tcctcgggcg cttccacgac atgcgcaatc gagatgcgtt
     6361 ctggtggtac ctgcggcgga cgatcgtgaa cctgtcgacc tcgtacttcc gccgtcgcgg
     6421 cgtcgaacgc gcgtggctcg cccgtcagcg tcccgacgag gcggcgcccg cgccccacga
     6481 tctcgccgag cgcgaccggc tgcggacggc gctcatgacg ctgcgcccgg agcagcgtgc
     6541 ggcgatcgtc ctgcgcttct acgaggacct gagcgaagcc gacacggcgg aggcgctcgg
     6601 catgccgctc ggcacggtga aatcggccgt gtcccgaggc ctcgaacgcc tccgccacga
     6661 gcttccggag gaggcgtgat ggacacgcgg ctgcggaccc tgctgcacga cctggcgagc
     6721 gagatgccgg tcgacatcga gggcacggca ccgaagacgc tgcgcagggc gcgggggcgt
     6781 cgggtcttga ccgccggcgc cggggcggcg atcgcaatcg cgctcgtcgt ggtcggcgtc
     6841 tccgcgatgc cgttcggcaa cgaggcgacg ctcacgccgg cggtgaccgg tcccaaccca
     6901 tcggcgacct tccagggtct gtggcccgag acgaacgccg acgcgctcgc cgagacccag
     6961 gcggccgtgg acgaggggca ctatccgctg caggccagcc ccgagggcat cgcgtcactc
     7021 ctcgcggtca acctcctggg ctgggagccg ggagacgatc gggccgaacg cgtggacgtc
     7081 cgcggcagcg aggccgaggt cgtgatcggc aatcggacgt tcgccgactc ggtggcgccg
     7141 gtgaacatcg agacgcgcca actcggtcgc accggaccgc gcggcgtctg gagcgtggtg
     7201 gacgtctcaa caccgctcat ccggctcgac ccggttgcgg agatcgcgcc cggcgtgatc
     7261 gggatctccg gcggcgtcac ccatcggtac gacggggcgg cctggatgga tgccaacgtc
     7321 ttcgacgggc cgaagccgga gccgtcgctg ggatcggctc ggtacgagct gaccgaccgg
     7381 acgttcgcgt tcgagatcca tgtctcgccg acgcccgacg ggcgggcgac gctgctcctc
     7441 acgatgccgg acgccgtcgg cgcgagcctc ggtgccgtca tggttcccgt tcccaccccg
     7501 gtcggcgagc ccgagccgct cgagggcccg aacctcgagg gcgttccgcc cgacgtcgcg
     7561 gtgaccgccc agcggatcta cgacgcggcg ctcgcgggcg acgtcgacgc gcttgccccg
     7621 ctgctcgatc cgaacacgtt cgcgtacaac ttcgacgacg gctcggaccc gatccccgcc
     7681 tggcgagcgg acccatcggt cctggatccc atggtcgcgg tgctcgagct gcccgccgcc
     7741 gaaccaagga cgatcgaggg ctacggcacc ctgaccatct ggccgtacct gatcgacagc
     7801 gacttcgacg cgctcaccga acgcgaggcc gacgacctcc aggccctcgg ctactccacg
     7861 gccgacatcc agctgatgat cgacggcggg aacggctacc agggaccgcg gctcgcgatc
     7921 gacgagaccg gcctgtggcg aagcttcacg acgatgggcg agtgagctgg gtcagggcag
     7981 ccgaacccgg cgacgcagga actccaggac cgcgccccac tcgcggacct gctcgtcgac
     8041 gatgtaggag ctgtggccct cgccgtacga gtagagctcg acgtcgccac ccgccttctt
     8101 cagggctcgg acgtatcggt agacctggct ggggacgcag cgggtgtcgt gctcgcccac
     8161 caggacgaac acgggcgccc ggacgtcctt cacgtacgtg atcggcgacc gttcggcgat
     8221 gaactcgggg atgtcgtgga cgacgccgcc ggcgagcgtc cggtcgtacg cctggagcga
     8281 cggcgcggaa tcgtcgtacg agtccaggta gtcgcccacc ggcaccccgg ccaccccggc
     8341 cgcgaacagg tcgcggtgga gccccaacgc cagcagcgtg atgtagccgc cccacgacca
     8401 gccaccgatc acgacgcgtt cgggatcgac gatcccacgc gcgacgaggt ccgcacgacc
     8461 ggcgacgacg tcctcgacct cggggaaccc gatgttcccg atgatgtgat cgcgccatcg
     8521 cctgccgaag ccgatcgagc cgcgatagtt caccatcgcg acggcgaagc cctcgtccac
     8581 gagcgactgg acgtccggga accaggtgtc gaggtacagc cagtccggtc cgccgtgcac
     8641 ctttacgtag agggggaacg ggccgtcgcc cggcggggtc acgagccatc cctgcaccac
     8701 gtcccgggca gggttgcgga acaaccactc ccggtagggg cggcccgttc ggacgccccc
     8761 gggcgcccgg ggcaagacct ccgccccggt gtcgtcgagc aggacgggcg cgcgatgccc
     8821 tcgtgagagc cggagccaca cgcggccgtc gggccggacg cgggcgccga ggatctcgcc
     8881 ccgcggatgg tcgatctcgg agagcgagcc tcgacccagg tcgacgcggt agagacgatc
     8941 acgtccgcgg aagaggtggg cgatcaggac ggaaccggcg tcgggccacc agtcgaccgg
     9001 caccgcctcc cccggcaggt ccagcggcag gtccttccgc tcgcctgtgg tcggggccca
     9061 cacgcccggc cgcagcaggt cctcgcgctc gtcgccgatc aggatccgct gatcgcccgg
     9121 gacgggagac cacgcgaacg cgcgcacgcc caggtccggc ccgtccgcga gctcgcccac
     9181 gacggcgccg gtgcgcgggt cgagcacgag cagcttgcgg tggatgttgt cgccgtcctg
     9241 cgccacctcg aggcacacca gcgtctcgtc cgcggacagc cccacgtggt cgaacccctc
     9301 gaggtgcagc tcggagtcgg agatcccgat aaggtcggga tgccgataga tctccttggc
     9361 cgcggccccg tcctcggaca cgtagacggc gaagccgtcg cggtcggcga gtacggcgac
     9421 ggcgagctcg cgtccgagcg agagtccctc gggccacccg acgggggcgt ccgggagaag
     9481 ctcgaccgga tcgccaccct cgaacggcac ggcgagccag cgcccggatt cgtccccggt
     9541 ggggtcggag aaccacacga cgcgcgagcc gtccgcggag atcgtcgcca ggctcacgcc
     9601 gacgggttcg tcggagcgtc ggacggggtc ggagccgggc tcccacgcgt acgcctggaa
     9661 cgacccctgc tcgtccgaca gcaagacgaa ccgatcgggc gcggccgggc cccaggcggg
     9721 gagcgttcga gtgggtgcgc ggaaccgtcg ttcccacagc ggttgagcgg gcatcggcgc
     9781 cgaccctagc agggtcgcga cggcgcgcgg cgccgcccga cggagcctac ggagggaaca
     9841 gcacgaccgg gacgctcgcg agcgcgcgtt cgccgtcctc gccgatcccg tagacctgga
     9901 tcgtccccgg ttggctgcgt tcgatgaagt agaagagctt cgtcgcgaac gtcccgcggc
     9961 acccgtcgcc gcagctcgcg cgcacgacga tcgcggcgag ctcggtgccg tcctcaccga
    10021 gcacccggac ggtgagtgcg gcgtcgacga cgtcggcggt gccggtgacc gagaccgggc
    10081 tgccgacctc gccgtccacg aacggggtct cgatcgcgat cgccgggtct tcgaccgcgg
    10141 tcctcacggc agccgacgaa cgggtggggg tcggcggcga ggacgtcaac ggttcggtga
    10201 cccgctccgc cgtgcacccc gccagcagga acccgagcac cagcaccccc gctaacctca
    10261 cccgttcgcc tctcatagca tggggatcga ggtggtcatg cgggacgaac tgtaaaggag
    10321 ccggcatgcg gacacggatc gcggtgatcg tcgggacttg cctcgcggcg cagctcgtgg
    10381 tggcgtccac gacgacggcc gccggggcgc ggatccgggc gcgcgaggtg gtgggcggcc
    10441 tgaaccagcc cgtcgcgttc acgttcgggc cgggtaggaa ggtctggtac gtcgagaagg
    10501 agacgggcca ggttcggatc cacgatctcg acaccgatgc cgaccgcctg ttcgtgacgg
    10561 ttgggggcgt gaacggcgac ggcgagcggg ggatgctcgg catcgcgctg cacccgagct
    10621 acccggccaa gccgctcgtg tacgtgtacg cgacgcgttc cgccgggggc cggctcagca
    10681 accagatcct gcgctaccgg gaccgccacg gttcgggcgt caaccgaacg gtgctgttca
    10741 cgagcgtcgc gggcggcagc ccgtaccaca acggcgggcg gatcgcgttc gggcccgacg
    10801 ggatgctcta cgcgatcgtc ggcgacgcgc acgactcctc gaacgctcag gacaccacca
    10861 acgaggac
//
LOCUS       DATBRK010000042         6617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1737335, whole genome shotgun sequence.
ACCESSION   DATBRK010000042 DATBRK010000000
VERSION     DATBRK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737335"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VIH70_01310"
     CDS             complement(<1..271)
                     /locus_tag="VIH70_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115031.1"
                     /translation="MPKVSRDSAPQRQDAGPVVDLRDEFGGYTVSFASLLEDMDATPF
                     MKGLPDDRCQCPHWGYVFKGKMTARYADRDEVFEAGDAFYTPPGHV"
     gene            complement(597..1814)
                     /locus_tag="VIH70_01315"
     CDS             complement(597..1814)
                     /locus_tag="VIH70_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8115032.1"
                     /translation="MTVDRLTADEVAERAGTTSEHIALLAKVGILEPEDDGTYPRRDV
                     LRARFVLGLDAMGIDANAVADALASGDLTLGYVEIGGRRPPRSNQTFAELSNELDIPF
                     VTLERIYVAFGLPRPTPDEFVREEDLPLMRFIPVLVGAGLSEGDVLRLARVWGDSARR
                     VAQFQSYYLHHSIEEPFRQRGLKDNAAFEAALREVGIRSGRSGEDLLGWLYRRHSEVF
                     ETEHLFDHVETALEDAGVHRKEPRQVEGCVFADLSGYTRLTEESGDAEAARVSLKLAE
                     LVNEIASRHRGAVVKMLGDGVHFHFRDPRDAVMASLEIVDTVHPARLPPAHVGVNAGP
                     MIYDEGDYFGRTVNIAARIASQAGPDQVFVGEDVLLHLTPGGFRLVEVGAFELKGIAK
                     PLILYEAIRDGDA"
     gene            complement(1864..2013)
                     /locus_tag="VIH70_01320"
     CDS             complement(1864..2013)
                     /locus_tag="VIH70_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEY8115033.1"
                     /translation="MAMVEDPVCGMRIDSEEAAGTLEYEGKRYYFCSQACHDAFEADP
                     ISYVK"
     gene            complement(2023..3123)
                     /locus_tag="VIH70_01325"
     CDS             complement(2023..3123)
                     /locus_tag="VIH70_01325"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8115034.1"
                     /translation="MTSGIGASSFDVVIIGGSQAGLAVGYHLAKRGLRFVILEAGPEI
                     GQVWRSRWDSLTLFTPAQYSGLPGMAFPLPKDTYPSKDDVVTYLQSYISAFDLPVRLN
                     AKVTSLTQRDGAYVVATADEEFTASHIVVATGPFQVPFVPPIAGDLDEAVFQIHSADY
                     RNPAQLPEGRVLVVGGGNSGFQIAEELAATGKVDLAVGKRVPSLPQRLLGKDLFWWLS
                     GIGFMKVSTDSRLGRKLAKRDVLIGSSARRLRRSGVTLRKRLASAGGKRAVFGDGSEQ
                     DVDAIVWATGYRPDFSWIDIPTIKDERGGIIHWRGVTDAPGVLFIGLTWQHTRGSALI
                     GFVHDDAAFIASRIGSRLENRPGLVETDSERT"
     gene            complement(3120..4859)
                     /locus_tag="VIH70_01330"
     CDS             complement(3120..4859)
                     /locus_tag="VIH70_01330"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY8115035.1"
                     /translation="MRKRLADAELRMLGYNGSIPGPTLHVDEGSEITVQVTNDGDVEA
                     TVHWHGLRLENRYDGVPHETQAPIPIGGTFTYTVQFPDAGFYWYHPHIREDFGLEMGL
                     YGTIIVEPADPSYWPAVNRQLSITLDDLRLEEGHIAPFHRSGLNYTAMGRFGNVMLIN
                     GETEFSGEADVGEVVRLYLVNTANTRIFNFALGGMRMKLVGGDSGRYEREMFVDEVML
                     APSERAILDVMFDTPGEARLEHRTPDHVYDLGVFVVRGDSTAAAASSFEALRVDPQLT
                     AEHRSIDHDIERPPDKVLAFVASMPLLYGDDVAPATSYACPMHPEVTSAEPGACPKCG
                     MQLLPVHSEGETPASYACPMHPEVTSSEPGTCPKCGMKLLPVRSEEPPPTSYVCPMHP
                     EVTSSEPGTCPKCGMKLVPADTLPAVEPKPSEDAVHHEHQEHEQNDGLEWEDLMPEIN
                     RASEPSNMRWMLIDRESGAENGAISWAFTVGDRVKIRLVNEMESDHPMHHPFHVHGAG
                     RFLVLSRDGEPESNLVWKDTVLVPAGRTVDVLLDVSNPGLWMAHCHIAEHAQSGMMFS
                     FNVSRRDPAEVAT"
     gene            complement(5510..6016)
                     /locus_tag="VIH70_01335"
     CDS             complement(5510..6016)
                     /locus_tag="VIH70_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8115036.1"
                     /translation="MRLRPIPQTRLEANLRLIDLLGPELAQADRELRQLFRGDPRVAR
                     LLPIPGIGFMTAAVVIAEVWDVTRFPSPQHLSSWAGLTPTERSSAEHTRRGHISKQGS
                     RWLRWAMVESASRPHLNDPVLRGFRDRIARRRGGKIAKVALARRLLTLCYYALRDEGG
                     CRAYPVPR"
     gene            complement(5925..6524)
                     /locus_tag="VIH70_01340"
     CDS             complement(5925..6524)
                     /locus_tag="VIH70_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8115037.1"
                     /translation="MVYVGVDLHRKSSHVAVLADDGELLASRRIASRPDEFLRVFGEL
                     EAQPVGVAFEATYGWGWFADLLSDVGIPAHMAHPLATKAISSARVKNDRVDAKTLAHL
                     LRTDLLPEAWAAPPEVRDARKLGRTRVSLARISSRLKCQTHALLADQAITPPMTDVFG
                     LGGRPVPSRRAVAPHPTDTPGGQPAADRSARPRARPGRP"
BASE COUNT         1131 a         2161 c         2160 g         1165 t
ORIGIN      
        1 ggacgtggcc gggcggcgtg tagaaggcgt cgccggcctc gaatacctca tcacggtcgg
       61 cgtaccgagc ggtcatcttg cccttgaaga cgtaccccca atgcgggcac tggcagcggt
      121 cgtccggaag ccccttcatg aaaggcgttg catccatgtc ttcgagaagc gatgcgaagc
      181 tcacggtgta gccgccgaac tcgtcgcgta gatccaccac cgggccggca tcctggcgtt
      241 gtggcgcgct gtcccttgag acctttggca ttccatcgct cctttcattt gggtgacgcg
      301 gctagatgac gagaggttac ggccgtcgtg agtgttcgac cccaccctct cgcgccttcg
      361 cgggcactcc ggccattgag ttcgcccgat cgacagggtg gtcgggaata gcgacactca
      421 ttaggagagc agcaccatca acgtattccg atttcaccgg cgatgagccg gccgcgctgg
      481 tcggcagctg ggcgtgaaag tgaacgcttc gccgccctca atatcgctcc actcccttcc
      541 cagggacatc gtcgaccgta tgcatcccca cgccgattcc cgcggtatcc atggagctag
      601 gcatcgccgt cacggatggc ctcgtacaag atcagaggct tcgcgatgcc cttcagctcg
      661 aaggcaccaa cctcgacgag acggaatccg cccggcgtga ggtgaagcaa gacgtcctcc
      721 ccaacgaaga cctgatcagg cccggcttgc gaggcgatcc gagccgcgat gttgaccgtc
      781 cgcccaaaat agtcgccctc gtcgtagatc atcggtcccg cgttcacgcc gacgtgggcg
      841 ggcggcaggc gtgcgggatg gacggtgtcc acgatctcga gcgaggccat caccgcgtcg
      901 cgtggatctc ggaaatggaa gtgcacgccg tcgccgagca tcttcacgac cgcacctcga
      961 tgccgcgatg cgatctcgtt gaccaactcg gcaagcttga gcgagacgcg cgccgcctcc
     1021 gcgtccccgg actcttcggt cagcctcgtg taaccggaga ggtctgcgaa cacgcagcct
     1081 tcgacctgcc gaggttcttt ccgatggacc cccgcgtcct cgagcgcggt ctcgacatga
     1141 tcgaagaggt gctcggtctc gaacacctct gaatggcgcc ggtagagcca gccgagcaga
     1201 tcttccccgg accgacccga ccgaatgccg acctcccgga gcgccgcctc gaatgctgcg
     1261 ttgtccttga ggccacgctg gcggaacggc tcctcgatgg agtggtgaag gtagtagctc
     1321 tggaactgcg cgacccgccg ggcgctatcg ccccagacac gcgccagccg caagacatcc
     1381 ccttcgctca gtccggcgcc gacgagcacg gggatgaacc tcatgagggg caggtcctcc
     1441 tctcgtacga actcgtcggg cgtcgggcgc gggagcccga acgcgacgta gatgcgttcg
     1501 agggtcacga acgggatgtc gagctcgtta cttagctcag caaaggtttg gttcgaacga
     1561 ggcggacgtc gccctccgat ctcgacatac ccgagggtca gatccccgga ggcgagtgca
     1621 tccgcgacgg cgttcgcgtc gatgcccatc gcatcgagac ccaggacgaa tcgggctcga
     1681 agcacgtctc gtcgcggata ggtcccgtcg tcttccggct cgaggatgcc caccttggcg
     1741 agcagcgcga tgtgttctga cgtcgtcccc gcacgctccg ctacctcatc tgcggtgagc
     1801 cgatctacgg tcatgctcga gcctgaacgt cagcgtgaat gagcaaggag agacccgccc
     1861 cgttcacttc acgtacgaga tcgggtctgc ctcgaaggcg tcgtggcagg cctgcgagca
     1921 gaagtagtac ctcttgcctt cgtactcgag ggtccccgca gcctcttcgc tatcgatccg
     1981 catcccgcac accggatcct ccaccatcgc catgcggtcc ccctacgttc gctcggagtc
     2041 cgtctcgacc agtccggggc ggttctcgag ccgcgagccg atgcgacttg cgatgaaggc
     2101 ggcgtcgtcg tgcacgaacc cgatgagcgc cgacccccgg gtgtgctgcc aggtgagtcc
     2161 gatgaacagc acaccgggag cgtcggtcac cccgcgccaa tggatgatgc cgcccctttc
     2221 gtccttgatc gttgggatat cgatccaaga gaagtcgggg cgataccccg tcgcccagac
     2281 gattgcgtcg acgtcttgct cgctcccgtc gccgaagacg gcgcgcttcc ccccagcgct
     2341 cgccagccgc ttcctcaggg tgacgccgga gcgccgaagt cgtctggcgc tcgatccgat
     2401 gagcacgtcg cgtttcgcca acttccgacc gagtcgcgaa tcggtactga ccttcatgaa
     2461 cccgattccg gacagccacc agaagagatc cttgccgagc agtcgctgcg gcaacgacgg
     2521 aactcgcttg cccacggcga gatccacctt cccggtcgcg gcgagttcct cggcgatctg
     2581 gaagccggag ttgcctcctc ccaccacgag tactcgcccc tcgggcagct gagccgggtt
     2641 tcgatagtcg gcgctgtgga tctggaagac cgcctcatcc agatcgccag cgatgggagg
     2701 gacgaagggg acctggaatg gtcccgtcgc gacgacgatg tggctcgccg tgaattcctc
     2761 gtccgccgtc gccacgacgt atgcaccgtc tcgctgggtg agtgacgtga ccttggcgtt
     2821 gagcctcacg ggaagatcaa aggcggatat gtacgactgg aggtacgtga cgacgtcgtc
     2881 cttcgacggg tacgtgtcct tcggcaatgg aaaggccatc cccggaaggc cgctgtactg
     2941 agcgggggtg aacagcgtga gcgagtccca tcgggaccgc cacacctgcc cgatctccgg
     3001 gccggcctca aggataacga acctcagacc acgcttcgcg aggtgatagc cgacggcaag
     3061 accggcttgg ctgccaccga tgatgacgac gtcgaacgaa gaagctccga tcccggaggt
     3121 catgtcgcca cctccgcagg atcgcgccgg gatacgttga agctgaacat catcccgctc
     3181 tgggcgtgct cggcgatgtg gcagtgcgcc atccagagac ccggattcga gacgtcgagc
     3241 aagacgtcca cggtccgtcc cgcgggcacg agcaccgtgt ccttccacac gagattcgat
     3301 tccggctcac cgtcccgcga cagcacgagg aagcgcccag ctccgtgtac atggaacggg
     3361 tggtgcatcg ggtgatccga ttccatctcg ttgacgagtc ggatcttcac tcggtcgccg
     3421 accgtgaagg cccagctgat ggccccgttc tcggctccgc tctcccggtc gatcagcatc
     3481 cacctcatgt tgctgggctc cgatgcacgg ttgatctccg gcatcaggtc ttcccactcg
     3541 agtccgtcgt tctgttcgtg ctcttggtgc tcgtggtgga cggcatcttc actcggcttg
     3601 ggctcgaccg ccggcagggt gtctgcaggt acgagcttca tgccgcactt ggggcaggtt
     3661 cccggttccg aggatgtgac ctccgggtgc atgggacaga cgtatgaggt cggaggcggc
     3721 tcctccgatc gaaccgggag cagcttcatg ccgcacttgg ggcaggttcc cggctccgac
     3781 gacgtgacct ccgggtgcat cggacaggcg taagaggccg gagtctcccc ctcggagtga
     3841 actgggagca attgcatccc gcacttcggg catgctcccg gctccgcgga cgtgacctcc
     3901 gggtgcatgg ggcaggcgta agaggtggct ggcgcaacgt catccccgta gaggaggggc
     3961 atcgaggcga cgaacgcgag cactttgtcc ggcggtcgtt cgatgtcgtg atcgatcgac
     4021 cgatgctcag cggtgagctg ggggtcgaca cgcaaggcct cgaaggacga cgccgcggcc
     4081 gctgtcgagt ctccccggac aacaaacacg ccgaggtcgt agacgtgatc cggtgttcga
     4141 tgctcgagcc gcgcctcccc gggagtgtcg aacatcacgt cgaggatcgc ccgctccgag
     4201 ggagcgagca tcacttcgtc gacgaacatc tctcgctcgt atcgaccgct gtcgccaccg
     4261 accagcttca tccgcatgcc accgagagcg aagttgaaga tccgggtgtt cgccgtattc
     4321 acgagataga ggcgtacgac ctcccccacg tccgcctcgc ccgagaactc ggtctcgccg
     4381 ttgatcagca tgacgttgcc gaaccgcccc atcgccgtgt agttgaggcc ggaacggtga
     4441 aagggcgcga tgtgaccttc ctcgaggcgc aggtcgtcga gcgtgatgct gagctggcgg
     4501 ttgacggccg gccagtacga cggatcggct ggctcgacga tgatcgtgcc gtacagaccc
     4561 atctccaggc cgaagtcctc gcggatgtgc gggtggtacc agtagaaccc ggcatcggga
     4621 aactggaccg tgtaggtgaa cgttcctccg atcgggatcg gtgcctgcgt ttcgtgtggc
     4681 accccgtcgt atcggttctc cagacgcaga ccatgccagt ggaccgtcgc ctcgacgtcg
     4741 ccgtcgttgg tcacctgcac ggtgatctct gacccctcat cgacgtgcaa cgtcgggccc
     4801 gggatcgaac cgttgtaccc gagcatgcgc agctcggcgt cggccaagcg cttccgcacg
     4861 ggctcgatgc gaaggtcgat tcggtcgccg tcgcggagtc tgatgagctc cgaacgcgcc
     4921 gcttgtgcga gtcccgccgt gtcagtcgag aacaggtcgt cggggctcgc ggacttcgtt
     4981 tcgatcgggt tcgtggttcc ggatgcgcgt tccatgtgtg ttctccccca tgcttcgtgt
     5041 cgagccctct gggatgtcgt gtcagcccac cccaaagctt gcgccggccg tggagccctg
     5101 atcggaggag atgcccccgg gccggctcct tccacctagc cgggttcgcc ttcagggtgc
     5161 cagcccaggt cttcgcccac aaccctcaca caacggtcgt cccgcattcg tcccgcgaac
     5221 gaccgggaga cctcaagaat gacgggaacg gctggagcat cgaatccgca ggtcatctat
     5281 gaaggaacgg gccggtgtca acggttcatc cgggcatcac ctccgggcgt ggtggcatgg
     5341 gatggggggt cgtggccggt gcatcagcga agggtcggtg gggccagtca tctattcggg
     5401 cgagatccca tgatggacgt gcggcctggg cggctcaatc agaagcggcg gccgtcccgg
     5461 aggaggccat gacctgcggc gagcgcgccc cgaccacgag cgacggtctt catcggggga
     5521 ccgggtaggc gcggcaccca ccctcgtcgc gaagggcgta gtagcaaagc gtgagcaagc
     5581 gtctggcgag cgccaccttg gcgatcttgc cccctcggcg tcgggcgatc cggtcgcgga
     5641 agccccggag cacgggatcg ttcaggtgcg gacgggaggc cgactccacc atcgcccaac
     5701 gcagccagcg cgatccctgc ttggagatgt ggccgcggcg ggtgtgctcg gcgctgctgc
     5761 gctcggtggg ggtgagccca gcccacgagg acaggtgctg cggtgagggg aagcgcgtga
     5821 cgtcccagac ctcggcgatg accacggccg ccgtcatgaa cccgatcccg gggatgggga
     5881 ggagtcttgc cacgcgggga tcgccgcgga acagctgccg cagctcacgg tcggcctggg
     5941 cgagctcggg gccgagcaga tcgatcagcc gcaggttggc ctccaggcgt gtctgtggga
     6001 tggggcgcaa ccgcacgtct tgaaggaacc ggtcgacctc ccaggccgaa gacgtcggtc
     6061 atcggcggcg taatcgcctg gtcggcgagc agcgcatggg tctggcattt caggcgggag
     6121 gagatcctgg caagcgagac ccgcgtgcgg cccagcttgc gggcgtcgcg cacctccggc
     6181 ggtgcagccc aggcctcggg cagcaggtcc gtacgcagca ggtgcgcgag cgtcttcgca
     6241 tcgacgcgat cgttcttgac cctggccgag gagatggcct tggtggcaag cggatgggcc
     6301 atgtgcgcgg ggatgccgac gtcggaaagc aggtcggcga accagcccca gccgtaggtc
     6361 gcctcgaacg cgacgcccac gggctgggcc tcgagttccc cgaagacgcg caggaactcg
     6421 tccggtcgac tcgcgatccg tcggctcgcg agcagctccc cgtcgtcggc gagaacggcg
     6481 acgtgagagg acttgcggtg cagatccact ccgacgtaga ccatgacagg gcctccttcc
     6541 tgtcgttcgt gtcgagcgga cgctacgacg tcacgctctg aacacgggag cggaggcccg
     6601 ctccttcatg gcatcag
//
LOCUS       DATBRK010000043         4060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1304344, whole genome shotgun sequence.
ACCESSION   DATBRK010000043 DATBRK010000000
VERSION     DATBRK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304344"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VIH70_01345"
     CDS             complement(<1..1108)
                     /locus_tag="VIH70_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY8115038.1"
                     /translation="MRRSASAGAVERRLWKDHPGARRIVVASTVVSLAASLCWVAFAL
                     ALSVVVARVFVDGGTLGSVGALLFAMLALVIARGGLLWGGEVVAQRAASGIKTDLRGR
                     LATALVALGPTAVRGERIGELVYTAGEGIESLDPYVTRYVPARALAVLVPILVAIVVF
                     VLDPWTVPILLIAGPVLVLLLGLIGRRVRDLAERRERELAWMNAHFLDVLRGLATLKM
                     FGRSAEQAETIRAVSRRQGSSTMDVLRTAFQTTLVLEWGATAATALVAIETSVRLMAG
                     GLAFERALAVLLLTPEFFLPLRRLAAEYHVGRSAAAAAEGVYAVVDAPARIHVPAPGA
                     SRPLPARMDVRFDDVVVTYDDGSRTALAGCSLEIP"
     gene            complement(1092..2351)
                     /locus_tag="VIH70_01350"
     CDS             complement(1092..2351)
                     /locus_tag="VIH70_01350"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8115039.1"
                     /translation="MPDRPRIVVLGGGFGGVGAVKKLGDADADVLLVDKHDYHTFQPL
                     LYQVATDLLEPPTVGHPLRDLFHDRPNVKVKQATVTGIDLDERRVQFAEMKPVSYDYL
                     VLGLGATVNFFGVAGAPKHAFPMYTLSDAVRLKEHVLERWTAADRDPDLIEDGALNVV
                     IVGAGPTGVESAGALAELYRQNLAKDYPDISQDQARIVVVEAGDAVLPMFKTKLQTYA
                     AKALEKRTVEIMLGEVVESIEPTRVRLKSGTVLNAHTLIWGAGLQANPLVSGLGVELQ
                     HGNRIPVGPDLTVPGHPEMYGVGDIAWITDTKTKTVLPQLGSVALQAGEHAGETISRT
                     LKKKKVEPFEYKDKGTMATIGRKAAVVQFRRGRSLTGTTAWLAWGTVHLALLSTGEDR
                     AKAVVDWTWAGFTHERSGRIQVDETER"
     gene            2608..2991
                     /locus_tag="VIH70_01355"
     CDS             2608..2991
                     /locus_tag="VIH70_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY8115040.1"
                     /translation="MSEPIEEFFERLSAGGPGMLPPRTSGSLRIDLGSDPGSGATTER
                     WFLTIGKDAVSVSHRNAKADAVIRTSRDLFEGMVTGRVNAMAGALRGVVSLEGDPTLL
                     VLFQRALPGPPAPAKRGTTAKARRS"
     gene            2988..>4060
                     /locus_tag="VIH70_01360"
     CDS             2988..>4060
                     /locus_tag="VIH70_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018732753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEY8115041.1"
                     /translation="MSDGVVKILDGNTFVVSDPRGDIEASLTDPTGLFSFDTRFLSTW
                     VLTVNGERLNALSVDDLQYFEARFFLVPGTGTVYVDAKLSVIRQRAVGDGFHEELTIL
                     NHAEEPVELTVRVDADSDFADLFEVKDALKKKGRYGNRIDKDGLHLVYERETFARVTT
                     ISASAPAAIDEKGLTFMVTIEPHGSWTTDLDVVTAAGAGGRLTRPKYRRGERRAQPNM
                     GRSLEKWLEEAPRLESEDDRLRATYRRSLVDLAALRFSPLVAGGRSLPAAGLPWFMTM
                     FGRDSILTSLQALPFTPELGATTLKALGDWQGSRIDDFRDEDPGRILHEMRYGEMTAF
                     EERPHSPYYGCADATPLYVVLLD"
BASE COUNT          638 a         1437 c         1393 g          592 t
ORIGIN      
        1 gtgggatctc cagcgaacag ccggcgaggg ccgttcgcga accgtcgtcg taggtcacga
       61 cgacgtcgtc gaaccggacg tccatccgcg ccggcagcgg gcggctcgca cccggcgccg
      121 ggacgtggat cctcgccggc gcgtcgacga cggcgtagac gccttcggca gcggcggcgg
      181 cggaacgccc gacgtggtac tcggcggcca ggcgccggag cggcaggaag aactcgggcg
      241 tgaggaggag caccgccagc gcccgctcga acgcgagccc gccggccatg agccggacgc
      301 tggtctcgat cgcgacgagc gccgtggcgg cggtcgcgcc ccactcgagg acgagcgtcg
      361 tctggaacgc ggtgcgcagg acgtccatcg tgctcgaacc ctgccggcgg ctcacggccc
      421 ggatcgtttc ggcctgctcg gcgctgcgcc cgaacatctt cagcgtggcc agcccacgga
      481 ggacgtcgag gaagtgggcg ttcatccagg cgagctcgcg ttcgcggcgc tcggcgaggt
      541 cgcggactcg gcgcccgatc agaccgagca acagcacgag caccggaccc gcgatcagca
      601 ggatcggcac cgtccacgga tcgagcacga agacgacgat cgccaccagg atcgggacga
      661 gcacggcgag cgcgcgcgca gggacgtagc gcgtgacgta cggatcgagg ctctcgatcc
      721 cctcccctgc cgtgtagacg agctcaccga tccgctcgcc ccggaccgcg gtggggccca
      781 gggcgacgag tgcggtggcc agccgaccgc ggaggtcggt cttgatgccg ctcgccgccc
      841 gttgggcgac gacctcgccg ccccacagga gcccaccccg agcgatcacg agcgccagca
      901 tcgcgaagag cagggcgccg accgacccga gcgtcccgcc gtccacgaag acgcgagcga
      961 ccacgaccga cagggccagc gcgaacgcga cccagcacag gctggccgcg agggacacaa
     1021 cggtcgaggc gacgacgata cgccgcgcgc ccgggtggtc cttccagaga cggcgttcga
     1081 cggcgccggc gctagcgctc cgtctcatcc acctggatcc ggccggaacg ctcgtgcgtg
     1141 aaccccgccc aggtccagtc gaccacggcc ttcgcacggt cctcgcccgt cgacaggagc
     1201 gccaggtgga ccgtgcccca cgcgagccac gccgtggtcc cagtgagcga cctaccccgg
     1261 cggaactgca ccacggcggc cttgcgaccg atcgtcgcca tcgtgccctt gtccttgtac
     1321 tcgaacggct cgaccttctt cttcttcagg gtccgcgaga tcgtctcgcc ggcgtgctcc
     1381 cccgcctgca acgccaccga gccgagctgc gggagcacgg tcttggtctt ggtgtcggtg
     1441 atccacgcga tgtcgcccac gccgtacatc tccgggtggc cggggaccgt gaggtcgggc
     1501 ccgaccggga tgcggttccc gtgctgcagc tcgaccccga ggccggacac gagcgggttg
     1561 gcctgcaggc ccgctcccca gatcagcgtg tgggcgttca ggacggtccc ggacttcagc
     1621 cgcacgcgcg tcggttcgat gctctcgacg acctcgccga gcatgatctc gacggtgcgc
     1681 ttctcgagcg ccttcgcggc gtacgtctgc aacttcgtct tgaacatcgg caggacggcg
     1741 tccccggcct cgaccacgac gatccgtgcc tggtcctgtg agatgtcggg gtagtccttc
     1801 gcgaggttct gccggtacag ctcggcgagc gcgcccgcgc tctccacgcc ggtcgggccg
     1861 gcgcccacga tgacgacgtt gagcgccccg tcctcgatca ggtccgggtc gcgatccgcc
     1921 gccgtccagc gctcgagcac gtgctccttc aggcgcaccg cgtcggacag cgtgtacatc
     1981 gggaaggcgt gcttgggggc gccggcgacc ccgaagaagt tgacggtcgc cccgagcccg
     2041 aggacgagat agtcgtagga gacgggcttc atctcggcga actgcacccg tcgctcgtcc
     2101 agatcgatgc ccgtgacggt cgcctgcttc accttgacgt tcggacgatc gtggaacaga
     2161 tcccggagcg ggtgcccgac ggtgggcggt tcgagcaggt cggtcgcgac ctggtacagc
     2221 agcggctgga acgtgtggta gtcgtgcttg tccacgagca ggacgtcggc gtcggcgtca
     2281 ccgagtttct tgaccgcgcc gaccccgccg aacccgcccc ccagcacgac gatccgaggc
     2341 cgatcgggca tcgtcgactc ctcctcccgc gtcgctcgaa accgggacgc gctctgtgaa
     2401 gcggccggac acgagcctag gtggaggccc aatcgaatcc cccccgtccg cgctcggcat
     2461 cgcccgaacc ggatgaacac gggagccgtg gcgggcgttg accgtactcc atttcatgcc
     2521 ttcggcatga tggcccggcc ggcacacgac ggttccatat ggaagtcggc cccgctggac
     2581 gatcggcccg cgcgcgtgga ggcatcagtg agcgaaccca tcgaggagtt cttcgaacga
     2641 ctctccgccg gtggccccgg gatgctcccg ccgcgcacaa gcggttctct gcggatcgac
     2701 ctggggtccg acccggggtc tggggcgacc accgaacgtt ggttcctcac gatcggcaag
     2761 gacgccgtct cggtctccca tcgcaacgcg aaggccgacg cggtgatcag gacctccagg
     2821 gacctgttcg agggcatggt caccggcagg gtgaacgcga tggccggcgc gctgcgtggc
     2881 gtcgtgtcgc tcgagggcga tcccaccctg ctggtgttgt tccagcgcgc cctgccgggg
     2941 cctccggcgc ccgcgaagcg agggacgacc gcgaaggcaa gacgatcgtg agcgacgggg
     3001 tcgtcaagat cctcgacggg aacaccttcg tcgtgagcga ccctcgcggt gacatcgagg
     3061 catcgctcac cgatccgacc ggcctgttct ccttcgatac ccgtttcctg tcgacgtggg
     3121 tgctcaccgt caacggcgag cgcctcaacg ccctgtccgt cgacgacctg cagtacttcg
     3181 aggcccggtt cttcctcgta cccgggaccg gcaccgtgta cgtcgacgcc aagctgtccg
     3241 tgatccgcca gcgggcggtc ggcgacgggt tccacgagga gctcaccatc ctgaaccacg
     3301 ccgaggagcc ggtcgagctc accgtacggg tcgacgcgga ctcggacttc gccgacctgt
     3361 tcgaggtcaa ggacgcactg aagaagaagg ggaggtacgg caaccggatc gacaaggacg
     3421 gcctgcacct ggtgtacgag cgcgagacgt tcgcacgggt gacgacgatc tcggcctcgg
     3481 cgccggccgc gatcgacgag aagggcctca cgttcatggt cacgatcgaa cctcacggct
     3541 cgtggacgac cgacctggac gtcgtgaccg ccgccggcgc cgggggtcgc ctcactcgtc
     3601 cgaagtaccg gcgcggggaa cgccgggctc agccgaacat gggccgaagc ctggagaagt
     3661 ggctcgagga ggcgcctcgc ctggaaagcg aggacgatcg cctgagggcg acctatcggc
     3721 gcagccttgt ggacctggcc gcgctccgct tctcgccgtt ggtcgcggga gggcggagcc
     3781 ttcccgctgc cgggctcccc tggttcatga ccatgttcgg ccgggacagc atcctgacca
     3841 gcctccaggc cctgcccttc acgcccgagc tgggggcgac gaccctcaaa gcactcgggg
     3901 actggcaggg cagccggatc gacgacttcc gcgacgagga tccgggtcgc atcctccacg
     3961 agatgcggta cggcgagatg acggccttcg aggagcgccc gcactctccc tactacggtt
     4021 gcgcggacgc cacgcccctg tacgtggtgc tgctggacga
//
LOCUS       DATBRK010000044         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2605963, whole genome shotgun sequence.
ACCESSION   DATBRK010000044 DATBRK010000000
VERSION     DATBRK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2605963"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..752)
                     /locus_tag="VIH70_01365"
     CDS             complement(78..752)
                     /locus_tag="VIH70_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY8115042.1"
                     /translation="MATPESRGRIALPTGEVGSVWIRPRDAAGTLVVAHGAGAGMDHP
                     FLAGFCRAMGEARVSTMRFNFPYIERGRRSPDPERVLRETWLAAFEAASEREDGRGVL
                     AGGKSLGGRMASMCAADGMPAAGLVFLGYPLHAPGKTEKLRAEHLERIRVPMLFLQGT
                     RDPFANPDLLKDVLSRLGKGAVLEPVEGGDHSFRIRGVKADDREIGAALAGAAAPFVR
                     RVAGER"
     gene            770..1477
                     /locus_tag="VIH70_01370"
     CDS             770..1477
                     /locus_tag="VIH70_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY8115043.1"
                     /translation="MDGNLYLGTSGFAYDEWKHGVFYPEGLKNREMLSYYASRFPSVE
                     INYTFRRFPTEKSMQTWKAETPETFRFTLKANQRITHWKRLADADQDVRDFLDLGRLL
                     GERLGTVLYQCPPTLEYDRGLIEAFVGYLPPAPRAAMEFRHPSWVEARDLLLDQGVAW
                     CVAETDENDPGPDDLSWEPFGFLRLRKTGYSDEELLGWVDRIRPSLEAGRDVYCYFKH
                     EDQGHSTQMADRLRAML"
     gene            complement(1506..>1874)
                     /locus_tag="VIH70_01375"
     CDS             complement(1506..>1874)
                     /locus_tag="VIH70_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY8115044.1"
                     /translation="REFRTTLRKAIEGRLSLDHRVREKRRHYPDPQVNVPVRVDIDND
                     ASDFFTVIEVGAPDRLGLLFDITRTFAELQLDVHLAKVATFGGRVVDAFYVRDDLGRR
                     VDDLERIRELEDALRGRLVP"
BASE COUNT          281 a          676 c          653 g          264 t
ORIGIN      
        1 ccaggccggc gccgccgact gcggcatgcg tgcgggctgt ggcccctcgt cgggggcccg
       61 gcggttgcgg cgcggcatca gcgctccccg gcgacccgcc ggacgaaggg ggctgcggcg
      121 ccggccagcg ccgcgccgat ctcgcgatcg tcggccttga cgccgcggat ccgaaacgag
      181 tggtcgccgc cctccaccgg ctcgagcacg gcccccttgc cgagcctcga gagcacgtcc
      241 ttgaggaggt cggggttcgc gaacggatcc ctcgtgccct gcaggaacag catcgggacc
      301 cggatccgct cgaggtgctc cgcgcgcagc ttctcggtct tgccgggcgc gtgcagcggg
      361 tagccgagga acaccagccc cgcggccggc atcccgtcgg ccgcacacat cgaggccatg
      421 cgcccgccga gggacttgcc cccggcgagg acgccccgtc cgtcctcgcg ttcggacgcc
      481 gcctcgaacg ccgccagcca ggtctcgcgc agcacgcgtt cggggtcggg cgaccggcgc
      541 ccgcgctcga tgtaggggaa gttgaaacgc atcgtcgaca cgcgcgcctc ccccatcgcc
      601 cggcagaatc cggcgaggaa cgggtgatcc atgccggcac cggccccatg cgcgaccacg
      661 agcgtgcccg cggcgtcccg tggacggatc cagaccgacc ccacctcccc ggtcggcaat
      721 gcgatgcgcc ctcggctctc cggcgtggcc atgccagaat ccttatcaga tggacgggaa
      781 cctctacctg gggacgtcgg ggttcgccta cgacgagtgg aagcacggcg tgttctaccc
      841 ggaggggctg aagaaccgcg agatgctctc gtactacgcg agccggttcc cctcggtcga
      901 gatcaactac accttccggc gcttcccgac cgagaagtcc atgcagacgt ggaaggccga
      961 gacccccgag acgttccggt tcaccctgaa ggcgaaccag cgcatcacgc actggaagcg
     1021 cctggcggac gcggaccagg acgtgcgcga cttcctggac ctcgggcggt tgctcgggga
     1081 gcggctcggg accgtcctgt accagtgccc gcccaccctg gagtacgacc gcgggctgat
     1141 cgaggcgttc gtcggctacc tgccgccggc gccccgggcg gcgatggagt tccgccaccc
     1201 gtcgtgggtc gaggcgcgcg acctgctgct cgaccagggc gtcgcctggt gcgtcgcgga
     1261 gaccgacgag aacgacccgg gacccgatga cctgtcctgg gagccgttcg ggttcctgcg
     1321 tctgcgcaag accgggtact cggacgagga gctgctcggc tgggtggacc ggatccgccc
     1381 ctcgctcgag gccggccggg acgtctactg ctacttcaag cacgaggacc aggggcacag
     1441 cacgcagatg gccgatcggc tccgggcgat gctctagccg aacgcgttcg cgccttgcgc
     1501 cgaggtcacg gtacgagccg gccgcgtagg gcgtcctcca gctcacggat gcgctcgaga
     1561 tcgtcgaccc tgcgcccgag gtcgtcgcgg acgtagaagg cgtcgaccac gcggccgccg
     1621 aacgtcgcca ccttcgcgag gtgcacgtcg agctgcagct cggcgaacgt gcgggtgatg
     1681 tcgaacagca gcccgagccg gtcgggcgcg cccacctcga tgacggtgaa gaaatcagac
     1741 gcatcgttgt cgatgtccac gcgcaccggg acgttgacct ggggatccgg gtagtgccgc
     1801 cgcttctcgc ggacacggtg atcgagcgac agccggccct cgatcgcctt gcgcagcgtc
     1861 gtccggaact ctcg
//
LOCUS       DATBRK010000045         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_584139, whole genome shotgun sequence.
ACCESSION   DATBRK010000045 DATBRK010000000
VERSION     DATBRK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584139"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..706
                     /locus_tag="VIH70_01380"
     CDS             134..706
                     /locus_tag="VIH70_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8115045.1"
                     /translation="MNDRPDGPLLAREEQQLVEGLLGGNEDAVRALYARYARPIFTLG
                     LRLLGSAEAAEELTQDVFLAAWRKAARFDPARGRLSTWLMTIAHNLAVDRLRRETGVT
                     RPHLVLVDEVPELEWVDEEEPLIERDAAIRALESLSVAEKRLLARAYFGGLTAREIAQ
                     ADGIPLGTVKTRLRTALIKVRRANQDKERP"
     gene            703..1473
                     /locus_tag="VIH70_01385"
     CDS             703..1473
                     /locus_tag="VIH70_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY8115046.1"
                     /translation="MTCLEVRELLPEHAIGVLGELERERIRRHLQTCAGCRKEAGDLG
                     QAASTLAFALPPARVPEGLREQVVARVRRAAGAPRTRRRARTATVGLVAAALVFASLG
                     WGAVMAGRAERFADRAAQAQREQAIAIEHFQQVLGNVIPGGAPSDNETHIGQLAPVAS
                     GTGGGFALQLVSPRILDFVMVIVNGLDDPSSLPMRVQLVNDRGRVLRAGRIDELDANG
                     GAEVYHQFRTQGLGGFTTVNVVDSDGRLVLTGTVDQSS"
     gene            complement(1484..2482)
                     /locus_tag="VIH70_01390"
     CDS             complement(1484..2482)
                     /locus_tag="VIH70_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115047.1"
                     /translation="MSQISTPQIPMPAPPPPPEAEAPGRRRGVTLVAVAVVLAVVAGL
                     VAFAITRSDTAEAQPLALSFTQGQERTYDVHQTMDATISSPLFGDQALSMDVKQVIGW
                     KVLSVDDAGTATIEVTVSDMSGTLNGTEVPSAPSPPIEIRIAADGRVLSAGGLSLGGA
                     GQTQGFGFPGMGQLTPILPDDGDAVSVGDTWDKEFSQDFPFGEGTIDFSATSTYVRNE
                     TVNGREAAVIQTQMTVPIDATLDLAELVDALGPEITGATGATGLDQLGDGSIAYLGRG
                     TFTQTSFVDLDAGELLKTQSRGRFDISMTLEGIPGLAEGPVEMSFAGSFTQGLELR"
     gene            complement(2537..3559)
                     /locus_tag="VIH70_01395"
     CDS             complement(2537..3559)
                     /locus_tag="VIH70_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY8115048.1"
                     /translation="MQPWSREFTGRIDELEIESEVLRGNALGDPNVRPIWVYVPPRYD
                     DEPDRRFPCVFVIQGLTGQLDMWRNREAFRPSYPENIDALFGSGEAPPTLIAFVDCWT
                     SIGGSQFLNSPGTGRYQDFLCDEVVAFVDGRYRTIPDRDHRGIQGKSSGGYGALVTPM
                     LRPDVFSALASHAGDALFEACYLPEFAKSARALRDKYGGSYDRFWEDFRARPAFSKEE
                     DSVLLNDWCMAACYSADDDGTVRLPFDVATGRLIEDVWQRWLDKDPVRMIPRFADQLR
                     SMRAIWVDGGTRDEWFLDNGAMAVSKELEAIGVEHTLELFDAGHMAIGYRYPRSLAFL
                     AQALAK"
     gene            3617..>4203
                     /locus_tag="VIH70_01400"
     CDS             3617..>4203
                     /locus_tag="VIH70_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8115049.1"
                     /translation="MPEVSDVVGIGLAGGRGMRARPLTLKAPGYLRSKAAMSFLGRRL
                     IRWVLQILSSEGIKEYYVIAHGKENRYQIKVMIGYGEGFGVDVTYSPVKYDALSTGSA
                     DSALRMLEYWDISQTALVFPTDTIIDFDLEPMLEAHRRTGAIATIAAMVREPDEVAEK
                     YGVLVADDQDRVEVFVEKPSLDDLREYFQASNEEE"
BASE COUNT          627 a         1460 c         1481 g          635 t
ORIGIN      
        1 cgcaccacgg ccggctcgcg gtgcatcggc ggtgggcgga cgagatccgg atgcccgacc
       61 tcatggcatg atgacgaaga cccaccgtca agctcgcggc ggtgcggcgg gcgtataccg
      121 ggaaggagtg cgggtgaacg atcggcccga tggtccgctc ctcgcccgcg aggagcaaca
      181 actcgtggag ggactccttg gtgggaacga ggacgccgtg cgtgcgctct acgcccggta
      241 cgcccggccg atcttcacgc tcgggctgcg gctgctgggg agcgctgagg cggccgagga
      301 gttgacgcag gacgtgttcc tcgccgcgtg gcggaaggcc gcgcgtttcg accccgctcg
      361 cgggcgcctg tcgacgtggc tcatgacgat cgcgcacaac ctcgccgtcg accgtcttcg
      421 ccgcgagacc ggcgtgaccc gcccgcacct ggtcctggtc gacgaggtcc ccgagctcga
      481 gtgggtggac gaggaggagc cgctgatcga acgcgacgcg gcgatccgcg ccctcgagtc
      541 gctctcggtc gccgagaagc ggctcctcgc gcgcgcgtac ttcggcgggc tcacggcgcg
      601 cgagatcgcg caggccgacg gcatcccgct gggcacggtc aagacacgcc ttcgcaccgc
      661 gctcatcaag gtccggcggg cgaaccagga caaggagcgg ccgtgacctg cctcgaggtg
      721 cgcgagctcc tgcccgagca cgcgatcggc gtgttgggcg agctcgaacg cgagcgcatc
      781 cggcgtcacc tccagacgtg cgccgggtgc cgcaaggagg cgggagatct ggggcaggcg
      841 gcgtcgacgc tcgccttcgc gctcccgccg gcccgggtac ccgagggcct ccgcgagcag
      901 gtcgtggccc gcgtgcgacg cgcggcgggc gcgccccgga cccggcgccg agcgcgaacg
      961 gcgacggtcg gcctggtcgc cgccgcgctc gtgttcgcga gcctcggctg gggcgccgtg
     1021 atggccggtc gcgccgagcg gttcgccgac cgggccgcgc aggcccaacg cgagcaggcg
     1081 atcgcgatcg agcacttcca gcaggtcctg ggcaacgtga tccccggggg agccccgtcg
     1141 gacaacgaga cgcacatcgg ccagctcgcg ccggtcgcat cggggaccgg aggcgggttc
     1201 gccctgcagc tcgtctcgcc gcggatcctc gacttcgtga tggtgatcgt gaacgggctc
     1261 gacgacccct cctcactccc gatgcgcgtg cagctggtga acgaccgggg ccgggtcctg
     1321 cgcgccgggc gcatcgacga gctggacgcg aacggcgggg ccgaggtcta ccaccagttc
     1381 cggacgcagg gcctcggcgg cttcacgacc gtgaacgtgg tggactcgga cggccgcctc
     1441 gtcctgaccg gcacggtcga ccagtcgagc tagcgttccg cgatcagcgg agctcgagcc
     1501 cctgcgtgaa cgagcccgcg aagctcatct ccacgggccc ttccgcgagt cccgggatcc
     1561 cctcgagggt catcgagatg tcgaaccggc cgcggctctg cgtcttcagc agctcccctg
     1621 catcgaggtc gacgaagctg gtctgggtga acgtgcctcg accgaggtag gcgatcgaac
     1681 cgtctcccag ctggtccagg ccggtcgcgc cggtcgcgcc ggtgatctcg gggccgagcg
     1741 cgtcgaccag ctcggcgagg tccagcgtcg cgtcgatcgg gacggtcatc tgggtctgga
     1801 tcacggcggc ctcgcgaccg ttcacggtct cgttgcggac gtaggtgctc gtcgccgaga
     1861 agtcgatcgt cccctcgccg aacgggaagt cctgcgagaa ctccttgtcc caggtatcgc
     1921 ccaccgacac cgcgtcgccg tcgtccggca ggatcggcgt gagctgcccc atcccgggga
     1981 acccgaaccc ctgcgtctgg ccggcgcccc cgagcgagag cccgcccgcg gacaggaccc
     2041 ggccgtcggc cgcgatccgg atctcgatcg ggggtgatgg cgccgacggc acctcggtcc
     2101 cgttcagcgt gccgctcatg tccgagaccg tgacctcgat cgtggccgtg ccggcgtcgt
     2161 cgaccgagag caccttccag ccgatcacct gcttgacgtc catcgacagg gcctggtctc
     2221 cgaacaaggg cgacgagatg gtggcgtcca tcgtctggtg aacgtcgtag gtccgctcct
     2281 gcccctgcgt gaaggacaac gcgagcggtt gcgcctcggc ggtgtcgctc cgcgtgatcg
     2341 cgaacgcgac caggccggcc acgacggcca gcacaacggc gaccgcgacg agggtcacgc
     2401 ctcggcgccg cccgggcgct tccgcctcgg gggggggcgg cggcgcgggc atcgggatct
     2461 gcggggtcga gatctggctc atcgcgggat ccttcctacg cgggcggggc ttccatcctc
     2521 ccacatcgga tcggtgttac ttcgcgagcg cctgcgcgag gaacgcgagc gaccgagggt
     2581 atcggtagcc gatcgccatg tgaccggcgt cgaacagctc cagcgtgtgc tcgacgccga
     2641 tcgcctcgag ctccttggag accgccatcg cgccgttgtc caggaaccac tcgtccctgg
     2701 tgcctccgtc gacccagatc gcgcgcatcg aacgcagctg gtccgcgaac ctcgggatca
     2761 tcctcaccgg gtccttgtcg agccatcgtt gccacacgtc ctcgatcaac ctgcccgtgg
     2821 ccacgtcgaa cgggaggcgg acggtcccgt cgtcgtcggc cgagtaacag gcggccatgc
     2881 accagtcgtt gaggaggacc gagtcctcct ccttggagaa cgcggggcgc gcgcggaagt
     2941 cctcccagaa gcgatcgtac gaacccccgt atttgtcgcg gagcgcgcga gcgctcttcg
     3001 cgaactccgg gaggtagcag gcctcgaaca gggcatcccc cgcgtgcgac gcgagcgccg
     3061 agaacacgtc gggccgcagc atcggcgtga cgagcgcgcc gtagccgccg ctcgacttgc
     3121 cctggatgcc tcggtggtcg cggtcgggga tcgtccgata ccggccgtcg acgaacgcca
     3181 cgacctcgtc gcacaggaag tcctggtagc ggcccgtccc cggcgagttg aggaactggc
     3241 tgccgccgat cgacgtccag cagtcgacga acgcgatcag tgtcggcggc gcctcgcccg
     3301 aaccgaacag cgcgtcgatg ttctccgggt agctcgggcg gaaggcctcg cggttgcgcc
     3361 acatgtcgag ctgacccgtc aggccctgga tcacgaagac gcaggggaac cggcgatcgg
     3421 gctcatcgtc gtatctcggc gggacgtaga cccagatcgg tcgtacgttg gggtcgccga
     3481 gcgcgttgcc gcgcaagacc tcgctctcga tctcgagctc gtcgatcctg ccggtgaact
     3541 cgcggctcca cggttgcacg gtcgtctcct tcgtgtcggg tgtgcgggct acggaaccct
     3601 cttatcatgg ccggacatgc ccgaggtgtc cgacgtcgtc gggatcggcc tcgcgggtgg
     3661 gcgggggatg cgtgcccgcc cgctgacgct caaggcgccc ggctacctgc gttcgaaggc
     3721 ggcgatgtcg ttcctcggtc gccgtctgat ccgctgggtc ctgcagatcc tatccagcga
     3781 aggcatcaag gagtactacg tcatcgcgca cggcaaggag aaccgatacc agatcaaggt
     3841 gatgatcggg tacggcgagg gcttcggcgt cgacgtcacg tactccccgg tcaagtacga
     3901 cgccctgtcg acgggcagcg ccgactcggc gctgcgcatg ctcgagtact gggacatctc
     3961 ccagaccgcg ctcgtgttcc ccaccgacac gatcatcgac ttcgacctcg agccgatgct
     4021 cgaggcgcac cggcggacgg gcgcgatcgc gacgatcgcc gcgatggtgc gcgaacccga
     4081 cgaggtcgcg gagaagtacg gcgtcctcgt cgcggacgat caggatcgcg tcgaggtctt
     4141 cgtcgagaag ccgagcctgg acgacctgcg cgagtacttc caggcctcga acgaggagga
     4201 gtt
//
LOCUS       DATBRK010000046         4580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_727017, whole genome shotgun sequence.
ACCESSION   DATBRK010000046 DATBRK010000000
VERSION     DATBRK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727017"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VIH70_01405"
     CDS             complement(<1..415)
                     /locus_tag="VIH70_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8115050.1"
                     /translation="MTERYDVVIVGGGHNGLTAAFYLARAGLRTIVLERREIVGGACV
                     TEEIAPGVRASTTSYIASMLRPEIVADLGLAKRGLRMVPCEPGLQVVLEDARVLPWWS
                     THERTAEEIRRISARDADAFARVTERLHELARSLQP"
     gene            585..2342
                     /locus_tag="VIH70_01410"
     CDS             585..2342
                     /locus_tag="VIH70_01410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8115051.1"
                     /translation="MSDDMRIRPARPEDAEAVAEVFIASFDTLIFLPKLHTDEVTIDF
                     IANKVMPEQEVLVAEEDGRVVGFVAMAHGNSLEHLYVHPDSQGRGVGGALLERAKERM
                     PAGFSLWVFQQNVQARRFYERHGLRLIERTEGAGNDERMPDARYEWRPNTRPPRLDGT
                     WRSGTASSRLHSAESRTRSQQEESIRIVLSGGRVFDGTGAPIGDADVSFEDGRIVDVG
                     PGLDGDESVDVAGATLLPGLFDCHVHIAERYEDFDELEVMHRPFSYRFFLIAENLRRT
                     LALGITTIRDAAGADLGLKRAVEDGILAGPRMRISVNALSMTGGHSDGWMPSGGLSVW
                     GVSYPGMPDAVCDGVEGVARKVREMVRAGADVIKISSTGGFLSPFDDPSRPHYSQDEV
                     DAIVAAAADLGRWVMSHAHGVEGIKRAVRAGARSIEHGTYLDRESAEMMVEHNTWLVP
                     TLTSGDTTEALARDPKVPELVREKLRELGRPELDAFRIAAEAGVKVAMGTDCPVAPHG
                     TNLRELTLMAENGFTPEQALVAATSSAADLMGVLDELGTLASGKRADVVVVDGDPLVF
                     ENISERIRSVYKDGVRVVG"
     gene            complement(2378..4003)
                     /locus_tag="VIH70_01415"
     CDS             complement(2378..4003)
                     /locus_tag="VIH70_01415"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY8115052.1"
                     /translation="MSDVDLVVRGGIVYDGTGRPPEVGDVAIRGDRITTVGDVGRLAA
                     RHVIDADGLAVAPGFINMMSWSNESLIEDGASQSEIRQGVTLEVMGEGWSMGPLTDEM
                     KAEVERRGAGNPLVEYSVEWTTLGEYLGWLERRGVSPNVASFVGASTVRIHAMGYEAR
                     PATEEELGRMRELVRQAMAEGALGVASALIYPPATSSTTQELVALAAVAAEFGGMYAS
                     HIRSEGAALIEAVEELLEIARLANVRAEIYHLKASGRANWPKLDGAIETIEAARARGH
                     AVTADAYPYTFSGTGLDACVPPWAHEGGFDVFLTRLRDPATRARILEGMRSSPSEWEN
                     PFLETAPDAIVVTGFREDALRHLTGMTLAEVASRGGTTPEETVLDLLVEDDNNVGALY
                     FAMAEDDVRKVLSLPWVSFCSDEESQAPEGIFLKADPHPRAYGAFARVLGTYVRDERI
                     LPLEEAIRRMTSLPAENLRLDGRGMLREGAYADVVVFDPAEVRDHATPDDPHRYSTGV
                     RHVLVNGSPVISEGEHTGARPGRFVRGPGVSAS"
     gene            complement(4015..>4580)
                     /locus_tag="VIH70_01420"
     CDS             complement(4015..>4580)
                     /locus_tag="VIH70_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY8115053.1"
                     /translation="AVALAGFVAEATSGADFDELCRQRVFAPLGMTDTGFRLADVETS
                     NLAMPYATVSHGDLEPIFHYGYPDYPDGALRASAVHLARWLGAFMNLGAFDGARVLDE
                     STVREIRRQQIPDLVGWHQGLIWYGQRRWGFFTLGHTGGDYGESTRMFFRPDRRVGVI
                     SFTNAYLGDGRWTAFRDIERRLFQEFS"
BASE COUNT          733 a         1565 c         1557 g          725 t
ORIGIN      
        1 acggctggag cgagcgggcg agctcgtgca gccgttcggt cacgcgcgcg aacgcgtccg
       61 cgtcgcgggc cgagatccgc cggatctcct ccgccgtgcg ctcgtgcgtc gaccaccacg
      121 gcagcacccg ggcgtcctcg agcacgacct ggagcccggg ctcgcacggg accatccgca
      181 gcccgcgttt ggccaggccg aggtcggcga cgatctcggg gcgcagcatg ctcgcgatgt
      241 aggaggtcgt cgatgcgcga acgcccggcg cgatctcctc ggtcacgcag gcgcccccga
      301 cgatctcgcg acgctcgagc acgatcgtcc gcaggccggc gcgcgcgagg tagaaggcgg
      361 cggtcagccc gttgtggccc ccacccacga tcacgacgtc gtaccgttcg gtcatcgttc
      421 ctcggccatc tcttggttga ctcggaggga cctcctcgtc catccgctcg ccgcagtctc
      481 gcacgagtca cgcgcaggaa gcactcaccg catgcacacg agcgtggcca cgtcgccgga
      541 tcaaggggcg agcgacgttc gctcggatcg tcaatagtgt tcgcgtgagc gacgacatgc
      601 ggatccgccc ggcacgacca gaggatgccg aggccgtcgc tgaggtgttc atcgcatcgt
      661 tcgacacgct catcttcctg ccgaagctcc acaccgacga ggtgacgatc gacttcatcg
      721 cgaacaaagt catgccggaa caggaggtgc tcgtcgcgga ggaggacggc agggtcgtcg
      781 gcttcgtcgc gatggcacat gggaactccc tggagcacct gtacgtgcat ccggattccc
      841 aaggccgcgg ggttggaggc gctttgctcg agcgggcgaa ggagcgcatg ccggctgggt
      901 tctcgctttg ggtgttccag caaaacgtgc aggctcggcg cttctatgaa cggcacgggc
      961 tccgcctcat cgagcggacg gagggggccg gcaacgatga gcggatgccc gacgcacggt
     1021 acgagtggcg accgaacacc cgacctcctc gtctcgatgg cacatggcgc tccggaaccg
     1081 cttcctctcg attacactcg gccgaatcgc gaacacgaag ccagcaggag gagagcatcc
     1141 ggatcgtctt gtcgggcggt cgcgtcttcg acggcacggg cgcgccgatc ggcgatgccg
     1201 atgtgtcgtt cgaggacggc cggatcgtcg acgtggggcc gggactcgac ggggacgaat
     1261 ccgtcgacgt ggcgggggcg acccttctgc cgggtctgtt cgactgccac gtccatatcg
     1321 ccgagcgcta cgaggacttc gacgagctcg aggtcatgca ccggcccttc tcgtaccggt
     1381 tcttcttgat cgcggagaac ctgcgtcgaa cgctcgctct ggggatcacg acgatccgcg
     1441 acgccgccgg agccgacctc ggcctcaaac gtgcggtgga ggacggcatc ctggcagggc
     1501 cgcgcatgcg gatctccgtg aatgcgctga gcatgaccgg agggcacagc gacggctgga
     1561 tgccgagcgg agggctgtcg gtgtggggcg tgtcgtaccc gggcatgccc gacgcggtct
     1621 gcgacggcgt cgagggcgtc gctcggaagg tgcgcgaaat ggtccgtgcc ggcgcggacg
     1681 tcatcaagat ctcctctacc ggcgggttcc tctcaccctt cgacgatccc tcccgaccgc
     1741 actactcgca ggacgaggtg gatgccatcg tcgccgcggc ggcggacctc ggacggtggg
     1801 tgatgtcaca cgcgcacggg gtggagggga tcaagcgagc ggtgcgcgcc ggggctcgct
     1861 cgatcgagca cgggacgtac ctcgaccggg agtccgccga gatgatggtg gaacacaaca
     1921 cgtggctcgt tcccacgctc acctccggcg acacgaccga agcactggcc cgagatccca
     1981 aggtccccga gctcgtccgc gagaagctgc gtgaactcgg acgtcccgag ctcgacgcgt
     2041 tccgcatcgc cgcggaggcg ggcgtgaagg tcgcgatggg aaccgactgc cccgtggcgc
     2101 cacacgggac gaacctccgc gagctgacgc tgatggccga gaacggcttc acgccggagc
     2161 aagccctcgt tgctgcgacg tccagcgcgg ccgacctgat gggcgtgctg gatgagctcg
     2221 gcacgctcgc ctcgggcaag cgcgcggatg tggtggtcgt ggacggcgac ccgctcgtgt
     2281 tcgagaacat ctccgagcgg atccgatccg tctacaagga cggcgttcgc gtggtcggct
     2341 gaccgaacgt cgagccagct cgcctgcgtt ccgccaccta tgacgccgag actccgggcc
     2401 cacgcacgaa acgaccgggc ctcgcgccgg tgtgctcccc ttcggagatg acgggcgacc
     2461 cgttcacgag gacgtgtcga accccggtgg agtatcggtg tgggtcgtcc ggggtcgcat
     2521 ggtcccgaac ctcggcgggg tcgaacacga ccacgtcggc gtaggccccc tcgcgtagca
     2581 tcccgcggcc gtccagccgg aggttctcgg ccggcaggct cgtcatccgc cggatcgcct
     2641 cctcgagtgg aaggatccgt tcgtcgcgca cgtaggtacc gaggacgcgg gcgaacgctc
     2701 cgtacgccct cggatgtggg tcggccttca ggaagatccc ctcgggggcc tgcgactcct
     2761 cgtcggagca gaagctgacc cacgggagcg agaggacctt ccgtacatca tcctccgcca
     2821 tcgcgaagta cagcgcgccg acgttgttgt cgtcctccac gaggagatcc agcaccgtct
     2881 cttccggtgt tgttcctccg cgcgacgcga cctcggcgag ggtcatgccc gtcagatgcc
     2941 ggagagcgtc ttcacggaac ccggtcacga cgatggcgtc cggcgcggtc tcgaggaagg
     3001 ggttctccca ctccgaggga gaggaacgca tcccctcgag gatgcgagcc cgcgttgccg
     3061 gatctctcaa tcgggtgagg aagacatcga acccgccctc gtgggcccag gggggcacac
     3121 acgcgtcgag tccggtgccc gagaacgtgt agggataggc gtcggccgtg acggcgtgcc
     3181 ccctcgcgcg cgcggcttcg atcgtctcga tcgctccgtc cagcttcggc cagttggctc
     3241 gcccgcttgc cttgaggtga tagatctcgg ctctgacgtt ggcgagccga gcgatctcga
     3301 gcagctcctc caccgcttcg atcaacgccg caccctcact ccggatgtgc gaggcgtaca
     3361 tccctccgaa ctccgcggcg accgcggcga gcgccacgag ctcctgtgtg gtcgaggagg
     3421 tcgccggggg atagatcagg gccgacgcga cgccgagtgc accctcggcc atcgcctggc
     3481 ggacgagctc gcgcatccgg ccgagctcct cctcggtggc gggtcgggcc tcatagccca
     3541 tcgcgtggat ccgaaccgtc gaggcgccga cgaacgaggc cacgttcggc gagacgcctc
     3601 gacgctcgag ccagccgagg tactcgccga gggtggtcca ctcgacggag tactcgacga
     3661 gcggattgcc tgcgcccctc cgctcgacct cggccttcat ctcgtcggtg aggggcccca
     3721 tggaccagcc ctcgcccatg acctcgagcg tgacgccctg gcggatctcg ctttgcgagg
     3781 cgccgtcctc gatcaacgac tcgttcgacc agctcatcat gttgatgaat ccgggcgcga
     3841 ccgcgaggcc gtcggcgtcg atcacgtgac gggcggcgag acgtccgaca tcgccgaccg
     3901 tggtgatccg gtcgccacgg atggcgacgt caccgacctc cggcggccgg ccggtgccgt
     3961 cgtacacgat cccgcctcgg acgaccagat cgacgtcgct catgccacga tcgatcagga
     4021 gaactcctgg aacaggcgac gctcgatgtc cctgaacgcg gtccaccggc cgtcgcctag
     4081 gtacgcgttg gtgaacgaga tgacccctac tcggcggtcc ggccggaaga acatccgcgt
     4141 gctctcgccg tagtcgcctc ctgtgtggcc cagcgtgaag aagccccacc gcctctgccc
     4201 gtaccagatc agcccttgat gccagccgac gagatcgggg atctgctggc gcctgatctc
     4261 gcggaccgtg gactcgtcga gcaccctggc cccgtcgaag gccccgaggt tcatgaaggc
     4321 gccgagccat ctcgccaggt gaaccgcgct cgcccgaagg gccccgtccg ggtagtcggg
     4381 gtagccgtag tggaagatcg gctcgagatc cccgtgggac acggtggcgt agggcatcgc
     4441 gaggttcgag gtctcgacgt cggcgagccg gaacccggtg tccgtcatcc ccaacggtgc
     4501 gaagacccgt tgcctgcaca gctcgtcgaa atcggcgccg ctggtcgcct ctgccacgaa
     4561 ccctgccagc gcgaccgcca
//
LOCUS       DATBRK010000047        14910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1593028, whole genome shotgun sequence.
ACCESSION   DATBRK010000047 DATBRK010000000
VERSION     DATBRK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14910
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593028"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..714)
                     /locus_tag="VIH70_01425"
     CDS             complement(91..714)
                     /locus_tag="VIH70_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8115054.1"
                     /translation="MTKVVIDMTMSLDGFVAGPDDGVADPLGRHGGEHLFDWYFSGSE
                     EYRDPVFRPEPGANLDEVRRMFAESGAFIFGRRTYEIAGGWGGRHPVNGVPVFVLTHD
                     PPADYPRGPSSLTFVTDGIESAIHQARTVADGKDIKLGGASPGKQALSAGLCDEILIH
                     LAPYLLGGGVRLFDTLPDGIHLEKLSVNDGPLATHLRYRVMPDPQPS"
     gene            complement(1015..2976)
                     /locus_tag="VIH70_01430"
     CDS             complement(1015..2976)
                     /locus_tag="VIH70_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY8115055.1"
                     /translation="MGTARTNARPRASELVSLAERMGSLGAVRGGLVALVLVYGLLSR
                     ETVEVSPAELTLVSAAYLLLVLLTPWIVRRQPRNRAQWVITLGLLVDGVYLSWATYAT
                     GGTLSPLRFLLIVHVVAVTLLASYRTGLKIAVWDSLLDLVVLYAQAADLVPVRESVAS
                     TSLAGGVNLKTAVLQLIPLWAAALVTATCAAASDRELRAQKVDLEELSAVVRDLDARA
                     SAAAIPEILLDALCRVFGFGRGVVLASPEGDLSLMAFRGPGDPPDLPPGRDLITERAW
                     NERRVQLASKLDPESDPRLAALLPGARNILVVPLLVDRGFRLGVVAVEHPSPRGQIKR
                     WVVVLVEQFASHAALTLQNAWLFDELERKLEENRALQIQLLSKNLDLEVKVAERTKEL
                     TKSLRKVQSVDEQRRRLLARLVHAEEDERKRIAGDIHDDPIQKIVATSMRLQLMRHQL
                     SDPEQLEVLDKLLSTVRSSIESLRHLIFELRPHALDEDGLGPALKDYLESIEAEFEFK
                     LEQRIEEEPPAELRVLLYRVAQEAITNIRKHAHARRVHVLLTDQDGGFLVRIADDGVG
                     FAAPDTLQSARGHLGVSAMRERVEMAGGWCQLRSLPGDGTTVEFWVPGVAPRAPEPSG
                     ADEGEFAVERQAEQDGSVPLSVGGPTTET"
     gene            complement(2981..3649)
                     /locus_tag="VIH70_01435"
     CDS             complement(2981..3649)
                     /locus_tag="VIH70_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115056.1"
                     /translation="MRALLLLAIGASLVVAAAPAGAACPLTDPACTETVDQVTEPVIE
                     PVTQPVTEPVNEVTDPVTGTVGGVVDKMKDTVDPIVDEVPVPPQLPDPSLDVNQPTTG
                     DSGNGPGPRTGPGAPPGDSGNGSSDPGTGSSVERPIPVQALVGGTFISGAAPSPNSDV
                     RIGPSPPADRTVLSLEDVVKGFAFPLALLIIVGAFLLVQDRLDRRDPRLALAPVGPDV
                     LEFA"
     gene            complement(3771..4976)
                     /locus_tag="VIH70_01440"
     CDS             complement(3771..4976)
                     /locus_tag="VIH70_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8115057.1"
                     /translation="MATAERIKVMVADDEQTVRDVLEALLGSEPEIDLIGAAADAETA
                     IELACSKLPDVALVDVHMPGGGGARATREILRRSPPTRVVALSAHEDVSTVMTMLRAG
                     AVGYVVKGDSTDEILSAIHRTVEGKTSVPERLAAPVASAMLEYIQGRRDASRTRQLQR
                     ERVERAIEDRSFRIVFQPIFDLESGHRVGLEALARFSDLPERPPNVWIAEAEAVGLLL
                     DLELALAKAALDQLETLPSDTYLSLNLSPETAVSERVRGLIEAADPSRIVVELTEHAP
                     VADYDELRESLSSLRERGVRLAIDDAGAGFASLRHIVRLDPDIIKLDITLTRRIEKDP
                     VRQALAVALVSFAKQVGATVVAEGVETELQLEALRRAGIRFVQGFLLGAPGPAPVADT
                     GQPLEELCR"
     gene            5329..6534
                     /locus_tag="VIH70_01445"
     CDS             5329..6534
                     /locus_tag="VIH70_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8115058.1"
                     /translation="MDRIRVLIADDEQAIRESLASVIESDPSLEIVGSAQDAQGAIDL
                     AAWRLPDVALLDVRMPGGGPRAAAEIARLSPSTRMLALSAVEDRESVLAMIRAGALSY
                     VGKTASNEEILSAIHGTAEGGTRLSPRAVDGVFEAIADTAEDDGSSNGNGSSLGRDPL
                     EEVVRIIERRAVELAFQPLADLQTLHVVAVEALPRFRTRPMRPPETWFTEAAKHGSLV
                     DLELVALTAAIGRMEMLPSDAFLAVSISSETAVSDRFRDLIRGAELNRIALQLKEYSA
                     GHDELPAGALDELRAGGVRVAVHHAGSGPWSLRHVALAPDLIKVDMSTLHEMSADPAG
                     HEPVSSFIAFATVVAAGVETEREIEMLRGLGIHLAQGNYLARAGQIPSGGGAWGRTTL
                     SAHPTAIAT"
     gene            7160..7813
                     /locus_tag="VIH70_01450"
     CDS             7160..7813
                     /locus_tag="VIH70_01450"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY8115059.1"
                     /translation="MTLVDLSHQIADGMAAYPGLPPVRIFPILDHDESRERYGGRAEF
                     HLGGAELAGNTGTYLDAPFHRFREREDLADLPLESVVGLPGLVVDHDGSERAVSLEEV
                     VETALRGRALLVRSGRDALWGSGEYWSDAPFIPAALADRLVAAGVGLVGVDFGNVDDM
                     DALARPAHTRLLDAGIPIVENLRGLDRLPRDGFVFSAPPIAIVRGAAFPVRAFAEVP"
     gene            7905..8093
                     /locus_tag="VIH70_01455"
     CDS             7905..8093
                     /locus_tag="VIH70_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115060.1"
                     /translation="MSKGGTGAAAGGGALYGLGIFGALFYYWQQADSFWEYVWAIFPK
                     AILWPAYMVYEGFRALGA"
     gene            8123..9529
                     /locus_tag="VIH70_01460"
     CDS             8123..9529
                     /locus_tag="VIH70_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8115061.1"
                     /translation="MTPPLHELERVIDGELVAGDELTTTRAPKPFNARFDGAIPQAVV
                     RCTSPGDVAETISFIRRHRLRSATRSGGHCLAGRSTTTGILVDVSPMRTVSVADGTLT
                     IGAGAVLGEVYAEAIRNAVTIAGGSCPSVGVAGLTLGGGLGILGRTYGVTSDRLVGAR
                     IVLADGRVLDCDEHHETDLFWALRGAGTGHFGVVTSLVFRPIPAPTVATTFHLEWPFA
                     DAASVAQAWMAWSPSAPDTLSASMVVAATAEPEEAPTVEVFGTMLGAVSEARHELEGL
                     TSLLGSDPSSTFVEEMGYEDTLRYWAARAGERLETPRAAPSTRSIHAVKSEYFTRPLP
                     TEAIAALLEGLTADRSRGQARELDFSPWGGAYVRIEPGATAFVHRDAAFWVKHGVVVP
                     AHATTIQQTVARDWVAASWGSLHPWGTGGVFPNFADADLEDWGHAYYGSNYERLVELK
                     GRYDPDNLFRFSQSLPVS"
     gene            complement(9539..10120)
                     /locus_tag="VIH70_01465"
     CDS             complement(9539..10120)
                     /locus_tag="VIH70_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8115062.1"
                     /translation="MGKVISEISMSLDGFVTGPNVRVGNGMGDGGDRLHDWKFDAKTE
                     TDEAIVDEIYASTGAVLIGKRMFDVGFEPWGDPPPFGMPVFIVTHERREPLPMQGGTT
                     YTFVTDGIETALELAHAAAGDENVGIWGGANVVRQYLEAGLLDEMQIHLIPILLGGGV
                     RLFEDLDPQGIELRRTGSIETPGATHMRFEVVK"
     gene            complement(10295..11350)
                     /locus_tag="VIH70_01470"
     CDS             complement(10295..11350)
                     /locus_tag="VIH70_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY8115063.1"
                     /translation="MTTRSASCILAGLLVLGGSAAAPAPNLTGYPNSVAAAGDSITRG
                     YNTGTVPFADQPGNSWSTGTRSSVRSHYLRILDAEPAISGRSFNEAVSGAKMQDLDAQ
                     VAVVNDRAVAYITILIGANDLCTRTVAGMTSLEAFRTEFEEAMAALSAGSPRVRIYVV
                     SIPNVYRLWVILKDDALARFTWRVFDICQSLLEDPRSTDTDDIARRRAVRQRTIAFNA
                     VLEQVCALYIHCRFDGRALFDDPFTSEDVSRRDYFHPSLQGQKRIAQVTWAATFDFTD
                     ATAPTSSAISVPAEGGLSVSLAATDDVGVAGIEYRLNLGAWQRYVAPFLLAAGENIRF
                     RAVDVNGNTEMTHVLTA"
     gene            complement(11737..12825)
                     /locus_tag="VIH70_01475"
     CDS             complement(11737..12825)
                     /locus_tag="VIH70_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115064.1"
                     /translation="MAERPRVLIVGGSGVFGRLLSHELLRTTTVDLILAGRGLSAGIA
                     ACRELGAPDRTSAIALDLGNPESLARAARGCLAVACAAGPFQELSRELPMAAVRAGAH
                     WLDIGDDEGWVVPVLDDATLDAAARDAGLAVIPGLSTVPALSGALARWGCERLPGADR
                     ARITLFIGNRNAKGAGAIASAMSSGFHRPTRVDLPFGHRIAYVGRSPDMEFLRRDLGL
                     NAEFRVVLEWAPAGLLVFGLGPIWSRLGARTRSRGARILAAISRPFGRFGSEGGCLQV
                     DLWDGKRRLSVTALSADQRLGILPCAMVLQRLLEGDLPERGVVHPAAWLSPPTRIGEL
                     VTRGVRLVTAPSSERVHPAAEGLPLDPE"
     gene            complement(12801..13442)
                     /locus_tag="VIH70_01480"
     CDS             complement(12801..13442)
                     /locus_tag="VIH70_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115065.1"
                     /translation="MVDVTPRHAAVAAYVAAAVNLTAGLVMLVALRHGIPAGENDLPA
                     RIAYVTGHTLAWRLGWLVWNLAAISLLGFFVALAATWRDRAPILCELALLCAAGGLAA
                     DLGAETILAAVSPGLTGETYAVVESIAVALTGHLGNGLYAVGGILLTWAGRRELPPGL
                     LWLAAAVWTAALWLSVATLASSAAGQFASAAVLMPMFVLWAGLTGRWLSDRAS"
     gene            complement(13553..13945)
                     /locus_tag="VIH70_01485"
     CDS             complement(13553..13945)
                     /locus_tag="VIH70_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115066.1"
                     /translation="MAAGIYNLVRGAFSVLDPQWFFRVSEMAAANHPQIFATLGMVIG
                     LYGILYLEVARVPERGWMIAAVGLAGKVLGPIGLIYLIVVGVWPAKTLVIAVTNDFIW
                     WPFFTLYLVDAWPAFREISRVADDETRA"
     gene            complement(14118..>14910)
                     /locus_tag="VIH70_01490"
     CDS             complement(14118..>14910)
                     /locus_tag="VIH70_01490"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY8115067.1"
                     /translation="VAGRGHGHGYREVDGRRQYYRTDGVLWDHDAAFRMATVNPILLS
                     AVGQCLGHPFVPINDSLVVKLPRSGVAIRWHQDPPYEGPEGLPDTFGIPNFDCDIYLD
                     EATTENGCLYGLAGYHLAGHVEVERFQEEELFNRPDAVALPMQPGDVLLHAISAPHGS
                     RANESETLRRVFYVHFMARETVETLHPEWIGSKPGFGERDIDRVRSMIQERASAGLAE
                     PGTEKVELTPDGLVFTGDPVTPLRHWGALIAAMTPEQRKAAKTLA"
BASE COUNT         2389 a         5071 c         5095 g         2355 t
ORIGIN      
        1 gagggacgac gcggacccga acgccgagta ggccaacgca cgcaaccgag cgacggtgca
       61 aacgggtgcg tccgtgccga ccgccgctgg ctaactcggc tgcgggtccg gcatcacccg
      121 gtatcgcagg tgggtcgcga gcgggccgtc gttgaccgac agtttctcca ggtggatccc
      181 gtccggcagt gtgtcgaaga gccggacgcc gccaccgagg agataaggcg cgaggtggat
      241 caggatctcg tcgcagagtc cggcggaaag cgcttgcttg ccaggggagg cacctccgag
      301 cttgatgtcc ttaccgtccg ccaccgtccg cgcctggtgg atggcgcttt cgatgccgtc
      361 cgtgacgaac gtgagactcg acggcccgcg cgggtagtcg gccggcggat cgtgcgtgag
      421 gacgaagacc ggcacgccgt tcacaggatg cctgccaccc cagccgccgg cgatctcgta
      481 ggtccgtcgc ccgaagatga acgcgccgct ctccgcgaac atgcggcgga cctcgtcgag
      541 gttggcgccc ggctcgggcc ggaagacagg atcccggtac tcctcgctgc ccgagaagta
      601 ccagtcgaag aggtgctcgc caccatggcg acccagcggg tcggctacgc catcatccgg
      661 accggcgacg aacccgtcga gcgacatcgt catgtcgatg acgaccttgg tcatgcggat
      721 ctccttggtc tgtggcaggt agggccggag tggtgcaagc atcccttcaa aacaggcgac
      781 cgtcagcttc ccgcatcagt tatgcggagc gaatcgttca ggtaccgagc tgaaccgtag
      841 cgatgggcaa acgaggcctg gaagatcacg aacgtctagt tcgtcaacca gtaaccgagc
      901 ctggcaccga gccggatgct ctctgttgat ccgcgagacg ggcggcccgc gtcgggtcgc
      961 ccggcttcga gtggtggaaa cccgagcctg tacgggtcgc ggtggttgag gtggttacgt
     1021 ctcggtcgtc ggaccgccga cgctcagcgg gaccgagccg tcctgctcgg cctgacgctc
     1081 gacggcgaac tcgccctcat ccgcccccga cggctccgga gctcgggggg cgacgccggg
     1141 aacccagaac tccacggtcg tgccgtcgcc gggcagactt cgcagctggc accagccccc
     1201 cgccatctcg acgcgctcgc gcatcgccga gaccccgaga tggcctctgg ccgactgcaa
     1261 cgtgtccggc gccgcgaacc cgacaccgtc gtcggcgatg cgaaccagga acccgccatc
     1321 ctggtcggtc agcaggacgt ggacgcgccg agcgtgggcg tgcttgcgga tgttcgtgat
     1381 cgcctcctgc gccacgcggt acagcagcac cctgagctcg gcgggcggtt cctcctcgat
     1441 gcgctgctcg agcttgaatt cgaactcggc ctcgatgctt tcgaggtagt ccttcagggc
     1501 ggggccgagt ccgtcctcgt cgagggcgtg agggcgcagc tcgaagatca ggtggcggag
     1561 gctctcgatc gagctgcgca ccgtcgatag gagcttgtcg aggacctcga gttgctcggg
     1621 atcggacagc tggtgccgca tgagctggag ccgcatgctc gtggccacga tcttctggat
     1681 gggatcgtcg tggatatcgc cggcgatccg cttgcgctcg tcctcctcgg cgtggacgag
     1741 gcgggccagc agcctgcgtc gctgttcgtc cacgctctgg accttccgaa ggctcttcgt
     1801 gagctccttc gtccgctcgg ccaccttgac ctcgagatcg aggttcttcg acaggagctg
     1861 gatctgaagg gcccggttct cctccaactt gcgctcgagc tcgtcgaaca accaagcgtt
     1921 ctgcagggtc agggccgcgt ggctggcgaa ctgctccacg agcacgacca cccaccgctt
     1981 gatctgaccc cgcgggctcg gatgctcgac cgcgacgacc cccagacgga accctcggtc
     2041 cacgagcagg ggtacgacga ggatgttcct cgctcccggg agcagcgcgg cgaggcgcgg
     2101 atccgactcg gggtcgagct tggacgcgag ctggacccgc cgttcgttcc aggcccgctc
     2161 cgtgatcagg tcgcgcccgg gcggcaggtc cggcgggtcg cccggccccc ggaaggccat
     2221 gagcgagagg tcgccttcgg gggatgcgag cacgaccccg cgaccgaagc cgaagacgcg
     2281 acagagcgca tcgaggagga tctcggggat cgcggcggcc gacgcacggg cgtccaggtc
     2341 ccgaacgacg gccgagagct cctcgaggtc gaccttctgg gctcgaagct cgcggtcgct
     2401 cgccgccgcg caggtcgccg tgacgagcgc cgccgcccat agggggatca gctgcagcac
     2461 cgcggtcttc aggttcacgc cgccggcgag cgacgtcgac gcgacggact cacggaccgg
     2521 cactaggtca gcggcctggg cgtacaggac caccaggtcc aggagggaat cccacacggc
     2581 gatcttcaga cccgtgcgat aggacgcgag cagggtgacg gcgactacgt ggacgatgag
     2641 taggaatcgg agcgggctga gggttccgcc ggtcgcgtag gtcgcccagg agagatagac
     2701 gccgtcgacc aacaggccga gcgtgatgac ccactgcgcg cggttgcggg gttgtcgcct
     2761 gacgatccac ggcgtcagca ggacgagcag gagatacgcc gcggacacga gggtcagttc
     2821 tgccggcgac acctccacgg tctccctcga gaggaggccg tagacgagga ccagcgcgac
     2881 gagcccgcca cgcacggctc ccagggaccc catccgctcc gcgagggaga cgagctcgct
     2941 cgctcgaggg cgcgcgttcg tccgcgcggt tcccacggcg tcacgcgaat tcgaggacgt
     3001 cgggcccgac cggcgcgagc gcgagcctgg ggtcgcggcg gtcgagacga tcctgcacga
     3061 gcaggaacgc ccccacgatg atcagcagcg ccaaggggaa ggcgaatccc ttcacgacgt
     3121 cctcgaggct gaggacggtc cgatctgcgg ggggcgaggg gccgatccga acgtcggagt
     3181 tcgggctcgg agcggcgccc gagatgaacg tgccgccgac cagagcctgt accgggatgg
     3241 ggcgctcgac ggagctgccg gtgcccggat ccgagcttcc attgccggag tcacccggcg
     3301 gagcgccggg gccggttcga ggaccggggc cgttgccgga gtcaccggtg gtcggttggt
     3361 tcacgtcgag ggatggatcg ggcagctgcg gcggcaccgg cacctcgtcg acgatcgggt
     3421 cgacggtgtc cttcatcttg tcgaccacgc ctccgaccgt cccggtgacc ggatcggtga
     3481 cctcgttgac cggttcggtg accggttgcg tgaccggctc gatgacgggc tcggtgacct
     3541 ggtcgacggt ctcggtgcag gcggggtccg tcagcgggca cgcagctcca gcgggagccg
     3601 ccgcgaccac gagggatgcc ccgatcgcca ggaggaggag ggcccgcaag gacgtgccga
     3661 acgtacgttc ccgggctctc gaactcgcag cagcagatat catcgcgcac cgcccgttcg
     3721 tccatccggt cagcgcacgc acgaccgcgt gaccgttccg gcctgttgtc ctatcggcac
     3781 aactcctcca agggttgacc ggtgtcggcg acgggcgccg gccccggtgc gccaagcagg
     3841 aacccctgga cgaaccggat cccggcccgc cggagcgcct cgagctggag ctcggtctcg
     3901 acgccctcgg cgacgacggt cgcgccgacc tgcttggcga aggacacgag cgcgaccgcc
     3961 agcgcctggc gcaccggatc cttctcgatc cgccgcgtca gggtgatgtc cagcttgatg
     4021 atgtccggat cgagccggac gatgtggcgc aggctcgcga agccggcccc cgcgtcgtcg
     4081 atcgccaggc gcaccccacg ctcacgcaga ctcgagagcg actcccgaag ctcgtcgtag
     4141 tcggcgaccg gagcgtgctc ggtcaactcc accacgatgc ggctcgggtc ggcggcctcg
     4201 atcaggcctc gcacgcgctc ggacaccgcg gtctccggag acaggttcag ggagaggtac
     4261 gtatcggagg gcagggtctc gagctgatcg agcgcggcct tcgcaagggc cagctccaga
     4321 tccaggagca ggccgaccgc ctccgcctcc gcgatccaca cgttcggtgg ccgctcggga
     4381 aggtccgaga acctcgcgag cgcctccaac ccgaccctgt ggccgctctc gagatcgaag
     4441 atcggctgga agacgatcct gaacgagcga tcctcgatcg ctcgctccac tcgctcgcgt
     4501 tggagctgcc gagtccgaga cgcatcgcgt cgcccctgga tgtactcgag catggccgag
     4561 gcgaccggcg cggccaggcg ctcggggacg ctggtcttgc cctcgacggt ccggtggatc
     4621 gccgacagga tctcgtcggt cgagtcgccc ttcaccacgt agccgacggc gcccgcccgc
     4681 agcatcgtca tcaccgtcga cacgtcctcg tgtgcggaca acgccactac cctggtgggc
     4741 ggcgaccgtc gaaggatctc gcgcgtggct cgcgctcctc ctccccccgg catgtgcacg
     4801 tccacgagcg caacgtccgg aagcttgctg caggcaagct cgatcgcggt ctcggcgtcg
     4861 gccgccgcgc cgatcaggtc gatctcgggc tccgagccga ggagggcttc gagcacgtcc
     4921 cgtacggtct gctcgtcatc cgcaaccatc accttgatgc gctctgctgt agccaactcg
     4981 gggcctcctc gcgtcggtga gtcctctctg catccggtat cggccgggcc gcatcgggga
     5041 acgtatggct gcgcgtgtcc atcctcacgt ggtcctcgag accctcgtgg cctcgaacga
     5101 cctgcgacct tcggccctcg gcgtttgcgt tcgccgcgtg tgtacgacca tcggtccgcg
     5161 ctcgatgccg gcctggatcg ttcgtccgcg ttccgacgcg gagcgggagc caccctgcag
     5221 catcgagctc ggttcgccga tacctggaac agggttgcgg cgaactcggc gcgcaggcga
     5281 gccgcacgct cacctcgacg aacgtctcgg ctcggaccgg aggccagggt ggatcggatc
     5341 agggtgttga tcgcggacga cgaacaggcc atccgcgagt cgttggccag cgtgatcgaa
     5401 tccgacccct ccctcgagat cgtcgggtcg gcgcaggacg cgcagggcgc catcgatctc
     5461 gccgcatggc ggctccccga cgtagccctg ctcgacgttc ggatgcccgg cggggggccg
     5521 cgggcggcgg ccgagatcgc ccggctgagc ccttccaccc ggatgctcgc gctgtccgcg
     5581 gtcgaggacc gtgagagcgt cctggccatg atccgggctg gtgccctgag ctacgtgggg
     5641 aagaccgcgt cgaacgagga gatcctcagc gcgatccacg gcacggccga gggggggacc
     5701 cggctctccc cgagggcggt cgacggcgtg ttcgaggcca tcgccgacac cgccgaggat
     5761 gacgggtcct ccaacggcaa cggatcgtcc cttgggaggg acccgctcga ggaggtcgtg
     5821 cggatcatcg agcgccgggc tgtcgagctc gcctttcagc cgctcgccga cctgcagacg
     5881 ctccacgtcg tcgccgtcga ggccctgccc cgtttccgga cccggcccat gcgtccgccg
     5941 gagacctggt tcaccgaggc cgcgaagcac ggatcgctcg tcgacctgga gctcgtcgcc
     6001 ctgaccgcgg cgatcggccg gatggagatg ctcccgtccg acgccttcct cgccgtctcc
     6061 atctcatccg aaacggccgt gtccgatcgc ttccgcgatc tcatccgagg agccgagctg
     6121 aaccggatcg cgctccagct gaaggagtac tcggccggcc acgacgagct gccggccggt
     6181 gcgctcgatg agctgcgcgc cggtggcgta cgtgtcgccg tccaccacgc cggatcgggg
     6241 ccgtggagcc ttcgtcacgt cgcgcttgcg ccggacctca tcaaggtgga catgtcgacg
     6301 ctccacgaga tgtccgccga cccggcgggc cacgagccgg tgtcgtcgtt catcgcgttc
     6361 gcgacggtgg ttgccgccgg cgtcgagacg gagcgggaga tcgagatgct ccgaggactc
     6421 gggatccatc tggctcaggg caactacctc gctcgcgccg ggcagatccc gagcggggga
     6481 ggcgcctggg ggcgcacgac gctgagcgcc catccgaccg cgatcgctac ctgaccacca
     6541 tccgaccgcg gctagtggcg gtgcttcacc gccggcgagg aatctcggag acacacgtcg
     6601 gtgcccccgt tcagctcctt gcgaacggcg acggcacggt agacaccggg gcgatcgctc
     6661 acgtgctgac ggaagacacc cgaggcgttc gacttgtccg tccctacacc ccgccacccg
     6721 ccgtgggtcg acgtcgctgc accttgacgg tcacgccgtc ctcgcagtcc tcgaacccgt
     6781 cctgtgccct gatccgaccc accgcgatcc ggcgggatca cggcgggaac ccgaatgctt
     6841 tcgtttccct tgtggggctc ttctctcgcg agggcggtct cggctccggc atgctccgta
     6901 gcccgaaaac ttcatcggcc acgcacggct cgagtactgg gtcgagggtg ctcgcgcccg
     6961 atcagatggg ggttgagggg tgagcagagc gagaccagga tggccgacca tcccccggtg
     7021 tgcgtggacg cacgatggtg ggccgttgac cggcatggca tcaccacggg cagcatcagc
     7081 cggagctttg tcggggctcc cccggctacg ctgcccccgg acgctcgatc gagggcaagg
     7141 agagcgtgag ggggatccga tgacgctggt cgacctttcg catcagatcg ccgacgggat
     7201 ggccgcctac cccgggctcc cacccgtgcg catcttcccg atcctcgacc acgatgagtc
     7261 tcgcgagcgc tacggcgggc gggccgagtt ccacctcggt ggggcagagc tggcgggcaa
     7321 cacgggaacc tacctggatg cgccgttcca tcggttccgc gagcgcgagg acctcgcgga
     7381 cctgccgctc gagtcggtcg ttgggctgcc gggtctcgtc gtagaccacg acggatccga
     7441 gcgcgcggtg tcgttggagg aggtcgtcga gaccgctctg cgcggacgcg cactcctggt
     7501 gcgcagcggg cgggatgcgt tgtggggctc gggggagtac tggtcggacg cgccgttcat
     7561 ccccgcggcc ctcgcggatc ggctcgtcgc ggcgggggtg gggctggtcg gcgtcgactt
     7621 cggcaacgtc gacgacatgg acgccctggc tcgccccgcg cacacccgcc ttctggatgc
     7681 cggcatcccg atcgtcgaga acctccgcgg tctggaccgc ctcccgcgag acgggttcgt
     7741 cttctcggct ccgccgatcg cgatcgtccg gggcgccgcg ttccccgtcc gggcgttcgc
     7801 ggaggtgccg tagccgcccg agcgtttcgt cgagcctccc gtgtgctcgg gagatcacct
     7861 tcgttcggat aaggtcctca ggacgatcga cgggagggga ggcgatgtcg aagggcggca
     7921 cgggcgccgc cgcgggtggt ggagccctct atggcctcgg gatcttcggc gcgttgttct
     7981 actactggca gcaggccgac tccttctggg aatacgtctg ggcgatcttc ccgaaggcga
     8041 tcctctggcc ggcgtacatg gtctatgagg ggttcagggc gctcggggcg tgacgccgaa
     8101 gggcgacgat gggggatccg tcgtgacacc gccactgcac gagctcgagc gggtcatcga
     8161 cggcgaactg gttgccggcg atgagctcac aacgactcgc gcgccgaagc cgttcaatgc
     8221 ccggttcgac ggggcgatcc cgcaggcggt cgtgcgatgc acgtctccgg gcgatgtcgc
     8281 cgagacgatc tccttcatcc gtcgccaccg gctccgttcg gcgacacgca gcggcggaca
     8341 ctgcctggcc ggtcgctcaa caacgacggg aatcctggtc gacgtctcgc cgatgcgcac
     8401 ggtctcggtg gctgacggca cgttgacgat cggcgccggc gccgttctgg gagaggtcta
     8461 cgccgaggcg atccgcaacg ccgtcacgat cgccggtggt tcatgtccgt cggtcggggt
     8521 cgcaggcctc acgctcggtg gtgggctcgg gatcctgggc cgcacgtacg gcgtgacctc
     8581 tgaccgcctg gtgggtgctc ggatcgtgct ggccgacggg cgggtgctcg actgcgatga
     8641 gcaccacgaa acggatctgt tctgggcgct ccggggggcc ggcaccggtc acttcggcgt
     8701 ggtgacgagc ctcgtcttcc gcccgatccc cgccccgacg gtcgccacga ccttccacct
     8761 ggagtggccg ttcgcggatg ccgcctcggt cgcgcaggcg tggatggcgt ggagcccgtc
     8821 ggcgcccgac acgctttcgg ccagcatggt cgtggccgcg accgccgaac ccgaagaggc
     8881 tcctacggtg gaagtcttcg gcacgatgct cggcgccgtc tcggaagccc ggcatgagct
     8941 cgagggcctc acctctctgc tcgggtcgga tccgagttcc accttcgttg aggagatggg
     9001 gtacgaggac acgcttcggt attgggccgc ccgggcgggc gagcggctgg agacgccgcg
     9061 ggcggcgccg tcgacccggt cgatccacgc cgtcaagtcg gagtacttca cgcgacctct
     9121 cccgacggag gcgatcgcgg cgctgctgga gggccttacg gcagatcgat ccaggggcca
     9181 ggcacgggag ctcgacttct caccctgggg aggcgcctac gttcggatcg agcccggggc
     9241 cacggccttc gtgcaccgtg acgcggcgtt ctgggtcaag cacggtgtcg tcgtcccggc
     9301 ccatgcgacc accatccagc agacggtcgc gcgggactgg gtcgcggcat cgtggggctc
     9361 actccatccc tgggggaccg gtggtgtgtt cccgaacttc gccgacgcgg acctcgagga
     9421 ctggggccac gcctactacg gctcgaatta cgagcggctg gtcgagctca agggccggta
     9481 cgaccccgac aacctgttcc gcttctccca gtcgctcccg gtgtcatgaa tgcgtgcttc
     9541 acttcaccac ttcgaatcgc atgtgcgtag cgcccggcgt ctcgatcgat cccgtccttc
     9601 gtagttcgat cccctgcggg tcgagatcct cgaagagccg gacaccgccg ccgaggagta
     9661 tggggatgag gtggatctgc atctcgtcca gcaacccggc ctccaggtac tgcctgacga
     9721 cgttcgcgcc gccccagatg ccgacgttct cgtcgccggc ggcagcgtgg gccagctcga
     9781 gtgcggtctc gatcccatcc gtcacgaagg tatacgtcgt accgccctgc atcggcagag
     9841 gctctcgtcg ttcgtgcgtc acgatgaaca ccggcatccc gaacggggga gggtcgcccc
     9901 acggctcgaa cccgacgtcg aacatccgct tcccgatcag gacggccccg gtcgaggcat
     9961 agatctcatc cacgatcgct tcgtcggttt ccgtcttcgc gtcgaacttc cagtcgtgca
    10021 ggcgatctcc tccgtccccc atcccattcc cgactcgaac gttcggtccg gtgacgaacc
    10081 cgtcaagcga catcgagatt tcggaaatga ccttgcccat cgcagtcctc ctctacggat
    10141 cgcctctgcg gccgctcagg tcgacgaatc gtcgtgtgtg gatctcgatg ttgtatcagg
    10201 gtaggtcgac cgaagaggtc ctcgagatgg aagatatttg acttacgctc tccagccccc
    10261 gaagagaaga acgtgaagcc caaagaggag gtcgtcaggc cgtgagaacg tgagtcatct
    10321 cggtattacc gttcacgtcg acagcgcgga agcggatgtt ctcgcccgct gccaacaaga
    10381 acggggcaac gtaccgctgc caggcgccga ggttgagccg gtactcgatg ccggccacgc
    10441 cgacatcatc ggtggcggcg agagatacgg aaagcccgcc ctcggctgga accgagatgg
    10501 ccgacgaggt cggcgcggtt gcgtccgtga agtcgaacgt cgccgcccac gtcacctgcg
    10561 cgatgcgctt ctgaccctgg agcgacggat ggaagtagtc acgtctggag acatcctccg
    10621 aggtgaatgg gtcgtcgaag agggcgcgcc cgtcgaaacg acagtggata tagagggcgc
    10681 agacctgctc gagcacagca ttgaaggcga tggtgcgttg cctcaccgcc cttcgacgcg
    10741 cgatgtcgtc cgtgtccgtg gagcgcggat cctcgagcag cgactggcag atgtcgaaca
    10801 cacgccaggt gaatcgcgcg agagcatcgt ccttcaggat cacccacagc ctgtagacgt
    10861 tcggaatgct cacgacgtag atccgtacgc ggggtgatcc ggccgagaga gcggccatgg
    10921 cctcctcgaa ctcggtccgg aaggcctcga gcgaggtcat ccccgcgacc gtccgcgtgc
    10981 agagatcgtt ggccccgatc agaatcgtga tgtaggccac ggcccgatcg ttgacgacgg
    11041 ccacctgagc atccaggtct tgcatcttcg cgccgctcac cgcctcgttg aagctcctgc
    11101 cggagatggc cggctcggcg tcgaggatgc gcaggtagtg gctccgcacc gagctccgcg
    11161 tgccggtcga ccaggagttc cccggctgat cggcgaacgg caccgttccg gtgttgtagc
    11221 cgcgcgtgat cgaatcgccg gcggcggcca cggagttggg gtagccggtc aggttcggcg
    11281 ccggtgcggc ggccgatcca ccgaggacga gcagcccggc caggatgcag cttgcagatc
    11341 gtgtcgtcat ccctcgcact ccattgtcca gaaccacgat gggttcggct cgtttggggg
    11401 ggatctctcg atctacgcgg gtcgaggctc accgagtcat cgttcgcaga cccttcttac
    11461 acgcttcctc tgacacacgt cgatggcggg gtcgccgatc gccctgtcgg gtctcctggc
    11521 gcccagagct tgtcgttccc gcaccgtctt tcatgagatc gtcaccagga ccgcgcgacg
    11581 acatggcccg gggaagccgg aggggccgga gagcccgaag gccctccggc catccccgtc
    11641 caccctccgt caggcctcgc ttgaaagacc gctacgcggc agccggccgg ggctcatcgc
    11701 ttcccgacgc ttcgcgggcg tgtggaatag ctcacctcac tcggggtcga gcggcagtcc
    11761 ttcggccgct gggtgaaccc gctcagagga aggagcggtc accagtcgca caccccgcgt
    11821 gacaagctcg ccgatcctgg tcggggggga cagccacgcc gcggggtgaa cgacgccgcg
    11881 ttctggcaga tcgccttcca ggagtcgctg cagaaccatc gcgcatggca agatcccaag
    11941 acgctggtcg gcgctgaggg cagtcacgct caagcgccgc ttgccgtccc acagatcgac
    12001 ctggagacat ccgccctccg aaccgaagcg accgaacggc cgggagatcg ctgcgaggat
    12061 gcgggcccct cgcgagcgcg ttcgagcgcc gaggcgagac catatgggcc ccaagccgaa
    12121 gaccaacagc ccggccggag cccattcgag gacaactcgg aactccgcgt tcaggccgag
    12181 gtcacgccgg agaaactcca tatcggggga ccgaccgacg tacgcgatgc ggtgaccgaa
    12241 gggaagatcc actcgggtcg ggcgatggaa cccgctcgac atcgcgctcg cgatcgctcc
    12301 ggcccccttc gcgttccggt tcccgatgaa cagggtgatc cgggccctgt cggcgcccgg
    12361 caggcgctcg cagccccagc gcgccagggc accggagagc gccggcacgg tcgacaaccc
    12421 cggtatcacc gccaatccgg cgtcccgtgc agcggcatcg agcgtcgcgt catccaggac
    12481 cggcacgacc cacccctcgt cgtcgccgat gtccagccaa tgggccccag ccctcacggc
    12541 cgccatcggc agctcacgcg agagttcctg gaaggggcct gcggcgcagg caaccgccag
    12601 gcagcctcgc gcggctcgcg ccagcgactc tgggttaccg agatcgagcg cgatcgccga
    12661 ggtgcgatcg ggggcgccga gctcccggca cgccgcgatg ccggctgaaa gcccgcgccc
    12721 ggcaaggatg agatcgacgg tggtcgtgcg gagcagctcg tgagacagaa ggcgcccgaa
    12781 gacgccggac ccgccgacga tcaggacgcg cggtcgctca gccatcgacc ggtcagcccc
    12841 gcccacagga cgaacattgg catcaagacg gcggcgctcg cgaactgccc ggcggccgag
    12901 gaggcgagcg tggcgaccga cagccacagg gcggcggtcc ataccgccgc cgccagccag
    12961 aggagccccg ggggaagctc ccgccggccc gcccacgtca ggaggatgcc tccgacggcg
    13021 tagagcccgt tgcccaggtg gccggtgagg gcgacggcga tcgactccac gacagcgtac
    13081 gtctcccctg taagcccagg ggagaccgcc gccaagatcg tctctgctcc aagatctgcc
    13141 gccaggccac ctgccgcgca gagcagcgcg agttcgcaca ggatcggcgc tcggtctcgc
    13201 caggttgccg ccagggcgac gaagaagccc agcaatgaga tggccgcgag gttccaaacg
    13261 agccagccca gccgccacgc gagggtgtgc cctgtgacat aagcgatccg ggcgggcaga
    13321 tcgttctcgc cggccgggat cccgtgtctc agcgcgacca gcatcacgag tcccgccgtg
    13381 aggttcaccg ccgccgcgac gtatgcggcg acagcggcgt gccgcggagt cacgtcgacc
    13441 atgtcgtgtc actcagtcta gagaaggaag ttctcgcgac atcgatccct tggtggacgg
    13501 ctcatcgaca cccaagccgc ccgggtgtcg tggtcgcgac cgtacgatca ggtcaagctc
    13561 gagtctcatc gtcagcgacg cgcgagatct cccggaatgc gggccaagcg tcgacgagat
    13621 acagggtgaa gaaaggccac cagatgaagt cgttggtgac tgcgatgacg agtgtcttcg
    13681 cgggccaaac gcccacaacg atcaggtaga tcaaaccgat tggtccgagg acctttcccg
    13741 cgagacctac cgcggcgatc atccaccctc gctccggcac ccgcgcgacc tccagataca
    13801 ggatcccgta caggccgatg accatcccga gggtcgcgaa gatctgcgga tgattcgcgg
    13861 cagccatctc cgacacacgg aagaaccact ggggatcgag aacagagaag gcgcccctga
    13921 cgaggttgta gatgccggcc gccacgaaga cgcgccgatg gaaagagcgt cgaggtatcg
    13981 accaatcgga tcggaccacg gcatggatga tgcgtgcttc acccactcga tggaactacc
    14041 cgtggtcttg agcagaccga tcgatgggcg tacgggcgcc acgggcgtcg tgggcagcct
    14101 cccggagttc agggcagtca ggccaaggtc ttcgccgctt tccgctgctc cggtgtcatg
    14161 gcggcgatga gggcccccca gtgtcggaga ggcgtcaccg ggtcgccggt gaagacgagc
    14221 ccgtccggcg tgagctccac cttctccgtg ccgggttcgg cgaggccggc gctcgcgcgc
    14281 tcttggatca tcgatcggac gcggtcgatg tctcgctcgc cgaagcctgg cttgctcccg
    14341 atccactccg gatggagcgt ctcgaccgtc tctcgtgcca tgaaatgcac gtagaagacc
    14401 cgtcgcaggg tctccgattc gttggccctc gacccgtggg gcgcgctgat ggcgtgcagg
    14461 agaacgtcac cgggctgcat cggaagcgcg actgcgtccg gccggttgaa cagctcctcc
    14521 tcctgaaagc gctcgacctc gacgtgaccg gccaggtggt agccggcgag cccgtacagg
    14581 cagccgttct ctgtcgtcgc ctcgtcgagg tagatgtcgc agtcgaagtt ggggatgccg
    14641 aacgtgtccg gcaggccctc cggtccctcg taggggggat cctggtgcca gcggatcgcc
    14701 acgccggacc gaggcagctt cacgaccagc gagtcgttga tcgggacgaa cgggtgaccg
    14761 aggcattgtc cgactgccga gagcaggatg gggttcaccg tggccatccg gaacgcggcg
    14821 tcgtggtccc acagtacccc gtcggtgcgg tagtactgcc ggcggccgtc gacctctcga
    14881 tagccgtgcc cgtgcccacg gccggcgacc
//
LOCUS       DATBRK010000048         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2459895, whole genome shotgun sequence.
ACCESSION   DATBRK010000048 DATBRK010000000
VERSION     DATBRK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2459895"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VIH70_01495"
     CDS             complement(<1..320)
                     /locus_tag="VIH70_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY8115068.1"
                     /translation="MERGRARDLGVVIGTYPPGPRNAVTDVEGVRVGHRTIVRDPSPD
                     GAGAVRTGVTTVFPHEGLPWIERVYAGTDILNGFGELIGINQIAEWGLLHSPVVMTSS
                     LAIGR"
     gene            462..>1721
                     /gene="hemA"
                     /locus_tag="VIH70_01500"
     CDS             462..>1721
                     /gene="hemA"
                     /locus_tag="VIH70_01500"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY8115069.1"
                     /translation="MAILALGISHRRADVELLERLAFADEDLVKAYRRAADDAAVDEA
                     VIVSTCNRVEIYGNVPSYHAGFQALKRVLCESRGVAPDDLAAPLYSHYEGDAAEHLLT
                     VASGLDSMVLGEPQILSQVREALRRAEAERAAGPSLAAVFDAAVNTGRRVRSETGVGA
                     APDVFVAAGADLGAEVLGGLEGRHAVVIGAGQMSALAVKQLRRRGVATVRVLNRSLER
                     ARALAERTSAEPGDLAELPGALAQADIAISATGSAGVVLTEALVRDVVEGRRRPLFVL
                     DLAVPRDVEPTAGRLGGITLVDIDGLRASVAVRAAETAADIERAHAIVADEVRRFSIR
                     RRAERLGPLFRALRARGDAAIDAELERFRSDLAALEPQEREAVTALARGVVAKLLHEP
                     IVRLKETPGTGTQDAYARALVELFGLEL"
BASE COUNT          233 a          639 c          618 g          231 t
ORIGIN      
        1 cggccgatcg cgagcgagct cgtcatcacg accggcgagt gcagcaggcc ccactcggcg
       61 atctgattga tcccgatcag ctcaccgaac ccgttcagga tgtcggtccc cgcgtagacc
      121 cgttcgatcc agggaaggcc ctcgtgcggg aagaccgtgg tcacaccggt gcgaacggcg
      181 cccgccccat ccggcgaggg gtcgcgcacg atcgtccggt gtcctacccg aacgccctcc
      241 acgtcggtca cagcgttccg cggcccgggc gggtacgtgc cgatcacgac ccccaggtcg
      301 cgggccctgc cacgttccat cccaggctcc tccgtcgtgc gttcgggtgc aggcgggcgg
      361 agatggtacc cggaccctcc cggacccacg ggtccgtcac gggccgggat cgtccggcgg
      421 ccgcaggact ccatccggag gcgcgtagaa tcccgagacc catggccatc ctcgccctcg
      481 gcatctccca ccggcgcgcc gacgtcgaac tcctcgaacg cctggccttc gccgacgagg
      541 acctcgtcaa ggcctaccgc cgggccgccg acgacgccgc ggtcgacgag gcggtcatcg
      601 tgtcgacgtg caaccgtgtc gagatctacg gcaacgtccc ctcgtaccac gcggggttcc
      661 aggcgctgaa acgcgtgctg tgcgagtcgc gcggcgtcgc gcccgacgac ctggccgcac
      721 cgctctactc gcactacgag ggcgacgccg ccgagcacct gctcaccgtc gcgagcgggc
      781 tggattcgat ggtgttggga gagccacaga tactgtcgca ggtgcgggag gcgctcaggc
      841 gcgccgaggc cgagcgggcc gcggggccgt cgctcgcggc cgtgttcgac gcggcggtca
      901 acaccgggcg ccgagtgcgc tccgagaccg gggtcggggc ggcgccggac gtgttcgtgg
      961 cggccggggc cgacctcggc gccgaggtgc tcggtggcct cgagggtcgg cacgccgtgg
     1021 tgatcggcgc ggggcagatg tccgcgctcg ccgtcaagca gctgcgcagg cgaggcgtcg
     1081 cgacggtccg agtgctgaac cgatcgctcg aacgcgcacg ggccctggcc gaacgcacgt
     1141 cggcggaacc aggcgacctc gcggagctgc cgggcgcgct cgcccaggcc gacatcgcga
     1201 tctcggcgac cggctccgcc ggtgtcgtcc tcaccgaggc actcgtgcgc gacgtggtgg
     1261 aaggcaggcg gcggccgctg ttcgtgctgg acctcgccgt tccgagggac gtcgaaccca
     1321 ccgccggccg actgggcggc atcacgcttg tcgacatcga cggcctccgc gcgtcggtcg
     1381 ccgtccgggc cgccgagacc gcggccgaca tcgagcgcgc gcatgcgatc gtcgccgacg
     1441 aggtgcgacg gttctcgatc cgccgccgcg cggaacgcct cggtccgctc ttccgcgccc
     1501 tgcgcgcacg cggcgatgcg gcgatcgacg ccgagctcga acgcttccgg tccgacctcg
     1561 cggcgctcga gccgcaggag cgcgaggcgg tcacggccct cgcgcgcggg gtcgtcgcca
     1621 agctgctcca cgagccgatc gtgcggctga aggagacccc cgggacaggg acgcaggacg
     1681 cctacgctcg agccctcgtc gagctgttcg ggctcgagct c
//
LOCUS       DATBRK010000049         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2606647, whole genome shotgun sequence.
ACCESSION   DATBRK010000049 DATBRK010000000
VERSION     DATBRK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2606647"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VIH70_01505"
     CDS             complement(<1..467)
                     /locus_tag="VIH70_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY8115070.1"
                     /translation="MLDVNFFDEIRIGLATADQIRAWSNGEVKKPETINYRTLKPEKD
                     GLFCERIFGPTRDWECHCGKYKRVRFKGIICERCGVEITRSKVRRDRMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDIAPKDLERIIYFAAYVITRIDTDRRHKELPEIEKEI"
     gene            complement(557..>2153)
                     /locus_tag="VIH70_01510"
     CDS             complement(557..>2153)
                     /locus_tag="VIH70_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY8115071.1"
                     /translation="EFRAAVDAGDVVLAEEAGVVEDVSADDIVIRERAGSMKTYKLQK
                     FRRSNQGTSLNQKPLVNEGDKVVRNQVIADGPSTEAGELALGRNLIVAYMPWEGHNYE
                     DAIIISERLVKDDVLTSIHIEEHEVDARDTKLGAEEITRDIPNVSEEILKDLDERGIV
                     RIGAEVNPGDYLVGKVTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGESGKVI
                     GVRVFTREDGDELPPGVNQLVRVYVAQKRKISDGDKLAGRHGNKGVISTILPEEDMPF
                     LADGTPVDIILNPLGVPSRMNLGQVLEAHLGWAGLVGFRKDGQDHEGPVYVSTPVFDG
                     AREHEILEAIRSAHPNKDGVRLVDDVGKAVLYDGRIGEPYEEPVTVGVAYILKLLHLV
                     DDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYALQELLTIKSDD
                     VLGRVKVYEAIVKGENIPEPGIPESFKVLIKEMQSLCLNVEVRSAEGEEIELKETDED
                     VFRAAEELGIDLSRREPAGADYD"
BASE COUNT          297 a          769 c          679 g          408 t
ORIGIN      
        1 tcgatctcct tctcgatctc gggcagctcc ttgtggcgcc ggtccgtgtc gatccgcgtg
       61 atgacgtagg cggcgaagta gatgatccgc tcgaggtcct tgggcgcgat gtccaggagg
      121 tagccgagcc ggctcgggac gcccttgaaa taccagatgt gcgtgaccgg ggcggccagc
      181 tcgatgtgcc ccatccggtc gcggcggacc ttgctgcgcg tgatctcgac cccgcaccgc
      241 tcgcagatga tgcccttgaa gcggacgcgc ttgtacttgc cgcagtggca ctcccagtcc
      301 cgcgtcggac cgaagatccg ctcgcagaac aggccgtcct tctccggctt cagcgtccgg
      361 tagttgatcg tctccggctt cttgacctcg ccgttggacc acgcgcggat ctgatccgcg
      421 gtggccaggc cgatccggat ctcatcgaaa aagttcacat ccagcacgta ggcggcctcc
      481 tcatgacctc accctgcacc atggacaaca cttccttgtt cccttctggt ccgtcctcgt
      541 ccctcccggc cagctgctag tcgtagtcgg cgccggccgg ctccctgcgg gacaggtcga
      601 tcccgagctc ctcggccgcc cggaagacgt cctcgtcggt ctccttgagc tcgatctcct
      661 ccccctcggc cgaacgcacc tccacgttca ggcagagaga ctgcatctcc ttgatcagga
      721 ccttgaagga ctcggggatc cccggctcgg ggatgttctc gcccttgacg atcgcctcgt
      781 agaccttgac ccggccgagc acgtcgtcgg acttgatcgt cagcagctcc tgaagcgcgt
      841 aggccgcgcc gtaggcctcg agcgcccaca cctccatctc gccgaaccgc tgcccgccga
      901 actgcgcctt cccgccgagc ggctgctggg tgatcatcga gtaggggccg gtggaccggg
      961 cgtggatctt gtcgtccacc aggtggagca gcttcaggat gtaggcaacc cccacggtca
     1021 ccggctcctc gtacggctcg ccgatgcgac cgtcgtacag gaccgccttg ccgacgtcgt
     1081 cgaccaggcg gaccccgtcc ttgttggggt gcgcgctgcg gatcgcctcg aggatctcgt
     1141 gctcgcgggc gccgtcgaac accggcgtcg acacgtacac ggggccctcg tggtcctgcc
     1201 cgtccttgcg gaatccgacc aggcccgccc acccgagatg cgcctcgagc acctgcccga
     1261 ggttcatccg ggacgggacg cccagcgggt tcaggatgat gtcgacgggg gtcccgtcgg
     1321 cgaggaacgg catgtcctcc tccggcagga tcgtcgagat cacgcccttg ttcccgtggc
     1381 ggccggcgag cttgtcgccg tcggagatct tgcgcttctg cgccacgtag acgcggacca
     1441 gctggttcac gccgggcggc agctcgtcgc cgtcctcacg cgtgaacacg cggacgccga
     1501 tcaccttacc gctctcgccg tgcgggacct tgagcgaggt gtcgcgcacc tcgcgcgcct
     1561 tctcgccgaa gatcgcgcgc agcagccgct cctcgggggt gagctcggtc tcccccttgg
     1621 gcgtgacctt gccgaccagg tagtcgccgg ggttcacctc ggcgccgatc cggacgatcc
     1681 cgcgctcgtc gaggtccttc aggatctcct cggacacatt ggggatgtcg cgggtgatct
     1741 cctcggcccc gagcttggtg tcgcgggcgt cgacctcgtg ctcctcgatg tggatcgagg
     1801 tcaggacgtc gtccttgacc agccgctccg agatgatgat cgcgtcctcg tagttgtggc
     1861 cctcccacgg catgtacgcc acgatcaggt tgcggcccag cgcgagctcg cccgcctccg
     1921 tggacggccc gtcggcgatc acctgattgc ggacgacctt gtcgccctcg ttgaccagcg
     1981 gcttctggtt gagggacgtg ccctgattgg atcggcggaa cttctggagc ttgtaggtct
     2041 tcatcgagcc ggcccgctcg cggatcacga tgtcgtcggc cgagacgtcc tccacgaccc
     2101 cggcctcctc ggccaggacc acgtctccgg cgtcgacggc ggcgcggaac tcc
//
LOCUS       DATBRK010000050         8672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1593171, whole genome shotgun sequence.
ACCESSION   DATBRK010000050 DATBRK010000000
VERSION     DATBRK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593171"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VIH70_01515"
     CDS             complement(<1..189)
                     /locus_tag="VIH70_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115072.1"
                     /translation="MTVLHHSQEIARPVADVFDVVIHAGDFASWNPTIKSSRQLSPDP
                     IGEGTTFEWDLRGFGAVRQ"
     gene            318..1949
                     /locus_tag="VIH70_01520"
     CDS             318..1949
                     /locus_tag="VIH70_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8115073.1"
                     /translation="MAGRIAELDQAHTAVERKAWSDAYQAFHVVDPSDLTPEDWERFS
                     DSAWWTGRMEESISARQKAYTGYVEAGDERPAAWMAARLCIEHFLRDEPAVGAGWFAR
                     MQRHAKDLPDCVELGFAALLEATVLRFTGDPASALPLAVRAHEIGQRFGDRNLRGMAI
                     HTQGLILLAQGRVVEGVALLDEAMTSVVAGELSDYFTGAVYCNVIGACLDIADIRRAG
                     DWAEAARAWAESLPPESPYPGMCRINRAQLANLRGEWSRAESEAIRATEELMAFSPAL
                     AAEALYETGEVRRRRGDLAGAEEAFERAHELGVEPQPGLALLRLAQGKVDAALTALRT
                     AVMGTSGNLLRQARLHGALADAAIAAGDLDGARAAVDEMDAIARESDAPVLAASAATV
                     RGSILLAESDAPGALESLRRAGALWQELRLPYESARTRTLYGLALRAAGDEDDARLEL
                     RAALAAFERLGAARDAARASDLLGGPAALPGGLTAREAEVLRLVASGKTNRDIAVELV
                     ISEHTVARHLQNIFGKLGVTSRSAAAAFAYEHDLT"
     gene            2126..3211
                     /locus_tag="VIH70_01525"
     CDS             2126..3211
                     /locus_tag="VIH70_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY8115074.1"
                     /translation="MVIGSGQAGLSAGYYLKRAGRRFLIVDANEHVGGSWLNRYDSLR
                     LFTPPWAVRLPGWAYPARGGPCPTKDEMIEYLQAYASRFDLPVRTGVQVDRISREGDR
                     YVLSAGAERVEADNVIVASGADRDPRIPAFSRELGPEIVQLHSSAYRNPTQLREGGVL
                     VVGAGNSGADISLDVAPSHPTWLSGPHRGHVPVDIDRWFAQHVAFRVIRFFGLHVLTI
                     RTRFGRKAAVKYASQGDMLVRVKPKQFLAAGIERVPRTVGVRDGRPVLEDGRVLDVAN
                     VIWCTGFRHDFSWIDLPIFGDDGELMHHRGVVTSEPGLYFVGLMFQYSAASSVIPGVA
                     RDARYVVGELAGRAPAPRPGAGARAAA"
     gene            3350..3988
                     /locus_tag="VIH70_01530"
     CDS             3350..3988
                     /locus_tag="VIH70_01530"
                     /inference="COORDINATES: protein motif:HMM:NF040123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6518 family protein"
                     /protein_id="HEY8115075.1"
                     /translation="MRSSSRLWLVSVLVGVGLGAFSLLADGIVGGRLVGILGNIASPW
                     GLAAFFVGRLATQPKRGAVAGALTLVVAVAVYYLGGALRGYTVGEVDLVWTAVALVAG
                     PVMGWSGAAIASEPERPPILSVAAPSAMLVAEAIFLAIDRTVWHYDLTAETYRLIDLA
                     VMVALVIGGLVLPVVFEPDRRRRRVVYVVVALAGVGGAVGFLLLREFIVGLV"
     gene            4044..4952
                     /locus_tag="VIH70_01535"
     CDS             4044..4952
                     /locus_tag="VIH70_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEY8115076.1"
                     /translation="MSTPQGGIFALGTSSHAYLEFDAPAGQRGRDLVDAISALREPRT
                     TMGGVNLVAGFRPELWREIAPDDVPADVAGFNEDVVGVDGFVMPATQHDAVLWLSGSA
                     YDVIFDTAREAIAALRDRASVAEETSSWPYRHDLDLTGFIDGSENPTLMDAPEVALVP
                     EGSPGAGGTILLLQKWEHDAPAWESLSVQEQERVIGRTKSDSVELEDRPDDSHVGSTD
                     QERFGEIFRRNMPYGTVTDHGTMFVGFAGEQRRLSAMLESMAGLTSGTRDALTRFTHA
                     LTGAYYFVPSTESVRARIGASPGSIP"
     gene            5045..5275
                     /locus_tag="VIH70_01540"
     CDS             5045..5275
                     /locus_tag="VIH70_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY8115077.1"
                     /translation="MSQATKLAGAAGDQDPRIGLRSVAALRKLVEQLEALQVRNARGL
                     GWSWQEIADELGVSRQAVHKKHARGRILARRR"
     gene            5278..5862
                     /locus_tag="VIH70_01545"
     CDS             5278..5862
                     /locus_tag="VIH70_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY8115078.1"
                     /translation="MFERFTTSARSVVTLAQREARELSHPNVGTQHVLLGILGAPDTV
                     GARALEALGIHERDVREDVRSLDRDPSAFSDRDADALRSLGIDLDEVRRTVEQTFGSG
                     ALERGATDGARSWAGHVPFTRGSKKALELALREAIHLGHRSIGTAHLLLGLVRDDGCS
                     AARILVARGADRERVRAAVLREIAAGGDRPGRTA"
     gene            6016..6642
                     /locus_tag="VIH70_01550"
     CDS             6016..6642
                     /locus_tag="VIH70_01550"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8115079.1"
                     /translation="MDRPTDTKANPPAVVRQPGRPRSATAHAAILDAAVALFVERGSD
                     GISMEAVAAMAGVGKATIYRRWSSKEDLVVDAVARVFTKAVRPDTGTVRQDLIESARE
                     LHRLMSSSLTGRVFPRMATEIAHRSPLGRLYGERIIGPRRAVFEEALRRGIERGELPE
                     AIDLELAIDQLVGTLLLRKLTGRLKRSDPAVADRAVDMLLTGLGAGGA"
     gene            6688..8016
                     /locus_tag="VIH70_01555"
     CDS             6688..8016
                     /locus_tag="VIH70_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY8115080.1"
                     /translation="MIGAGSSGLAALKVLGEQGVAVECFERGSDLGGLWRYENDNGLS
                     GAYASLRTNVSRRRMQYPSFPMPRSPIDFPTHTEMAAYLDAYADASGLRELIRFGTTI
                     ERMEPDPGGRWNLSLDDGAVRTYNAVVVAIGLFWSPRIPDYPGSFDGEASHSHDYRTP
                     GSCAGRNVLVVGAGQSAAEIAVEVAGVANRTVMSVRSAHHVIPRWIGDRPYDADDVGP
                     LNRMPWRLMNLLFGRRSARELGQVPSSWPVASHRVLEGIPVVSSDLLPRVRRGEIVIK
                     PAVDRLLGDRVRFVDGSEEAVDRIVYATGYRISLPFLSSSLVSASGRDFPLYRRILPP
                     DVGGLFFVGFVDAPGGLLPVVEAQAEWVAAVLTGRLGLPSPERMRRRMERAERRTRQR
                     FPGEGPHSTRCDPHGYRRLLRSDLRRARRRRPRATGIRWSLGGSVRETAR"
     gene            8236..>8672
                     /locus_tag="VIH70_01560"
     CDS             8236..>8672
                     /locus_tag="VIH70_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115081.1"
                     /translation="MTAEPRRVGRHRHLDLVGEDPRERGHVGTFEGAQVAMEDRAFLT
                     GRRADGSSLVVPSSDARSPALQRAVDRRGRRAEELRDLGRAPLEHVSKKQDRALPRRQ
                     HVDRGEERELDRFPARESSRGILELVQEPVRVRLEPDGLEDGFA"
BASE COUNT         1217 a         2919 c         3219 g         1317 t
ORIGIN      
        1 ctgccggacg gcgccgaacc cccgcaggtc ccactcgaac gtcgtcccct ccccgatcgg
       61 atcgggcgag agctgacgcg agctcttgat ggtggggttc cacgaggcga agtcgccggc
      121 gtggatcacg acatcgaaca cgtcggcgac cggtcgggcg atctcctgcg agtggtggag
      181 gaccgtcatg gctcgagcgt cagcctacgc cggtccgtcc ggctccatcg atcgccgaca
      241 tgcggatgtg cgtcccgcgc catcggcata ggctgttccg gattccggcg cagggcgttc
      301 gacaccaggg aggcggagtg gccgggcgca tcgccgagct cgaccaggca cacaccgcgg
      361 tggagcggaa ggcgtggtcc gacgcgtatc aggcgttcca cgtcgtggac ccctccgacc
      421 tgacgccgga ggactgggag cggttctccg actcggcctg gtggaccggc cgcatggagg
      481 agtcgatctc ggcccggcag aaggcctaca ccggctacgt ggaggcgggc gacgagcgcc
      541 cggccgcgtg gatggcggcg cggctgtgca tcgagcactt cctccgggac gagccggcgg
      601 tcggcgccgg gtggttcgcc cgcatgcagc gacacgcgaa ggacctgccc gactgcgtcg
      661 agctcggttt cgccgcgctc ctggaggcga ccgtcctacg gttcaccggc gacccggcct
      721 cggcgcttcc gctcgccgtg cgcgcccacg agatcgggca gcgtttcggg gaccgcaacc
      781 tccgggggat ggcgatccac acgcaggggc tcatcctgct cgcgcagggc cgggtggtcg
      841 agggggttgc cctgctcgac gaggcgatga cgtcggtcgt cgccggcgag ctgagcgact
      901 acttcacggg cgccgtctac tgcaacgtga tcggcgcgtg cctggatatc gccgacatcc
      961 gacgcgccgg cgactgggcc gaggccgcgc gggcgtgggc cgagtcgctc ccgcccgagt
     1021 cgccatatcc ggggatgtgc cggatcaacc gggcccagct cgcgaacctt cgtggggaat
     1081 ggtcgcgggc cgagtccgag gcgatccggg ccacggagga gctcatggcc ttcagccccg
     1141 cgttggccgc tgaggccctg tacgagaccg gcgaggtccg ccggcgacgc ggcgatctcg
     1201 cgggcgcgga ggaggcgttc gagcgggcgc acgagctggg ggtcgagccg caaccggggc
     1261 tggcgctgct ccggctcgcg caggggaagg tggacgccgc gctgacggcg ctccgcacgg
     1321 ccgtcatggg cacgtcgggc aacctgctcc ggcaggccag gctgcatggg gccctggccg
     1381 atgccgcgat cgcggccggt gacctggacg gggcgagggc ggccgtggac gagatggacg
     1441 cgatcgctcg cgagtccgat gccccggtgt tggcggcgtc cgcggccacg gtccgcgggt
     1501 cgatcctgct ggccgagagc gacgcgcccg gcgccctgga gagcctgcgt cgagcaggtg
     1561 ccctgtggca ggagctccgc ctgccgtacg agtcggcgcg cacacggacg ctctacggcc
     1621 tcgcgcttcg cgccgccggc gacgaggacg atgcccggct cgagctgcgg gcggcgctcg
     1681 ccgcgttcga gcggctcggg gccgcgcgcg acgccgcgag ggcatccgac ctgttggggg
     1741 gtccggcggc cctgcccggc gggctcacgg ctcgcgaggc cgaggtcctg cgcctggtcg
     1801 catcgggcaa gacgaaccgc gacatcgccg tcgagctggt gatcagcgag cacaccgtcg
     1861 cccgccacct gcagaacatc ttcggcaagc tcggcgtgac ctcgcgctcg gcggccgcgg
     1921 ccttcgcgta cgagcacgac ctgacctgac gccgggacgg cgtggtcaga aacaaccatc
     1981 cgacacgctg cgaaagatgg ctgcttccgg cgatgagctg gtccggcccg tcctcctacc
     2041 gttcgcccat cggacagggc caatcggaca tggccagatc ggacatgaaa ggggtatcga
     2101 gacggacgcg gagcggttgg acacggtggt gatcggatcc gggcaggccg gcctgtcggc
     2161 cgggtactac ctgaagcggg cgggccggcg cttcctcatc gtggatgcga acgagcacgt
     2221 gggcggctcc tggctcaacc ggtacgactc gctgcggctg ttcacgccgc cctgggccgt
     2281 tcggctgccg ggctgggcct acccggccag gggcggcccg tgcccgacga aggacgagat
     2341 gatcgagtac ctgcaggcct acgcgtccag gttcgatctg cccgtgcgga cgggcgttca
     2401 ggtcgatcgg atctcgcgcg agggcgaccg gtacgtcctc tccgccggtg ccgagcgggt
     2461 cgaggccgac aacgtgatcg tggcctcggg ggccgaccgc gacccgcgga tccccgcctt
     2521 ctcccgggag ctgggccccg agatcgtgca gctccactcg agcgcgtacc ggaacccgac
     2581 ccagctccgc gagggcgggg tgctcgtcgt gggtgccggc aactccgggg ccgacatctc
     2641 gctggacgtc gccccgtcgc atccgacgtg gctctcgggc ccccatcgag gacatgttcc
     2701 ggtggatatc gaccggtggt tcgcgcagca cgtcgccttc cgggtcatcc ggttcttcgg
     2761 gctccacgtg ctgaccatcc ggacgcggtt cgggcggaag gccgcggtga agtacgcgtc
     2821 ccagggcgac atgttggtcc gggtcaagcc caagcagttc ctcgctgccg gcatcgagcg
     2881 cgtgccgcgg accgtcggcg ttcgagacgg caggccggtg ctcgaggacg gccgggtgct
     2941 cgacgtggcg aacgtgatct ggtgcacggg gttccgtcac gacttctcct ggatcgacct
     3001 tccgatcttc ggagacgacg gcgagctcat gcaccaccgg ggcgtcgtga cgagcgagcc
     3061 gggcctgtac ttcgtcgggc tgatgttcca gtactcggcg gcctcgagcg tgatcccggg
     3121 cgtcgcgagg gacgcgaggt acgtcgtcgg agagctcgct ggacgcgcgc ctgcgccgcg
     3181 cccgggcgca ggcgcgcggg ccgccgcctg acccggtcga cgttcccgtg agggcgggtc
     3241 gggcctctgc gacgatgagt cgtttcggta cccgacccgg ggaggacgtg gggtcattcg
     3301 cttaccagga caacggcagg gatcggttcc cgaccagggt gtgcccgcgg tgcgttcgtc
     3361 cagccgcctg tggctggtct cggtcctcgt cggcgtgggg ctcggggcct tctcgctgct
     3421 cgcggacggg atcgtcggcg gtcggctggt cgggatcctc gggaacatcg cctcgccgtg
     3481 ggggctcgcg gccttcttcg tcggccgcct cgcgacgcag ccgaagcggg gagccgtcgc
     3541 gggcgccctg acgctggtgg tggccgtcgc ggtgtattac ctcggcggtg cgctccgcgg
     3601 gtacaccgtc ggcgaggtcg acctcgtctg gacggcggtc gcgctcgtgg ccgggcccgt
     3661 catgggatgg agcggcgcgg cgatcgcgtc cgagcccgaa cgaccaccca tcctctcggt
     3721 ggccgcgccg tcggcgatgc tcgtggccga ggcgatcttc ctcgcgatcg accgcacggt
     3781 ctggcactac gacctgacgg ccgagacgta ccgcctcatc gacctcgccg tcatggtcgc
     3841 gctcgtgatc ggcggcctgg tgctccccgt ggtgttcgag ccggatcggc gccggaggcg
     3901 cgtcgtctac gtggtggtcg ccctggccgg ggtgggcggg gcggtcggat tcctgctgct
     3961 ccgggagttc atcgtggggc tcgtgtaggg acggccgaac gctcgagtgt gcagcgggcg
     4021 cgactcgcgg tacggtcggc gacgtgagca caccgcaggg cgggatcttc gcgctcggga
     4081 cgtcgtccca cgcgtacctc gagttcgatg cgcctgcggg gcaacggggg cgagacctcg
     4141 tggatgcgat ctcggcgctg cgcgagccgc ggaccacgat gggtggcgtc aacctggtag
     4201 caggcttccg gcccgagctc tggcgggaga tcgcgcccga tgacgtcccc gcggacgtcg
     4261 cggggttcaa cgaggacgtc gtcggcgtcg acggcttcgt gatgccggcc acccagcacg
     4321 acgccgtcct gtggctatcg gggagcgcct acgacgtgat cttcgacacg gcccgcgagg
     4381 cgatcgccgc actccgcgat cgagcgtcgg tcgcggagga gacatcgagc tggccctacc
     4441 ggcacgacct ggatctgacc ggcttcatcg acgggagcga gaaccccacg ctgatggatg
     4501 cgcctgaggt cgccctggtc cccgaaggca gtcccggagc cggcggcacg atcctgctcc
     4561 tgcagaagtg ggagcacgac gcgccggcgt gggagtccct ctcggtccag gagcaggagc
     4621 gcgtgatcgg acgcacgaag tccgacagcg tcgaactcga ggaccggccc gacgactcac
     4681 acgtggggag caccgaccag gagcgcttcg gcgagatctt ccggcgcaac atgccgtacg
     4741 ggacggtcac cgaccacggg acgatgttcg tcggcttcgc gggggagcag cggcgcctct
     4801 cggcgatgct cgagagcatg gccggcctca cctccggcac tcgcgacgcg ctcacgcgat
     4861 tcacgcatgc gctcaccggc gcctactact tcgtgccctc gaccgagagc gttcgtgcgc
     4921 ggatcggggc ctcgccgggc tcgatcccct aagcccgaac ccgaaccgat cggccgtgcg
     4981 ggatggcgtg cgcgggtgtc aatcagggtt gacaaggatc ctgtgtcaac gtacattgac
     5041 gggcatgagt caggccacga agctcgcggg agccgccgga gatcaggatc cgaggatcgg
     5101 gctccgctcg gtggccgcgc tccgcaagct cgtcgagcag ctggaggcgc ttcaggtgag
     5161 gaacgcgcgc ggcctcggat ggtcgtggca ggaaatcgcg gacgaactcg gcgtcagccg
     5221 ccaggccgtc cacaagaagc acgcgcgggg tcggatcctc gcgcggagga ggtagcggtg
     5281 ttcgagcgct tcaccacgtc ggcgagatcg gtcgtcacgc ttgctcaacg ggaggcacgc
     5341 gagctcagcc atccgaacgt cgggacgcag cacgtcctgc tgggcatcct gggcgcacct
     5401 gacacggtgg gggctcgggc gctcgaggcg ctcgggatcc atgagcgcga cgtgcgtgag
     5461 gatgtccgga gtctcgatcg agacccatcg gccttctcag atcgggacgc ggacgccctg
     5521 cggagcctcg gcatcgatct ggacgaggtg cggcggacgg tcgagcagac cttcggctcg
     5581 ggcgcgctcg agcgaggcgc cacggacggg gcccgctcgt gggcggggca cgtccccttc
     5641 acacgggggt cgaagaaggc gctcgagctc gcgctccgcg aggcgatcca tctcgggcac
     5701 cggtccatcg ggaccgcgca cctcctgctc ggcctggtgc gggacgacgg gtgctcggcg
     5761 gcgaggatcc tggtggcacg cggcgcggat cgcgagcgtg tgcgggccgc cgtgctccgc
     5821 gagatcgcgg ccggaggcga tcgacccggc cgcaccgcct gatcgaaggc tcaggacctc
     5881 aaacgagatg caagccagcg gtgacccttg cgcgcgtgct ggcttggtgg tacgtttgtc
     5941 gtacgatacg agacggttcc gtatcgtatt cctcttttga ggctgacggc aaagcagcga
     6001 gattgaggct gggggatgga tcgaccgacc gacacgaagg cgaacccgcc ggccgtcgtt
     6061 cggcaacccg ggcgtccccg aagcgcgacg gcccacgcgg ccatcctgga cgccgcggtg
     6121 gccctgttcg tcgagcgggg atccgacggt ataagcatgg aggccgtcgc cgccatggcc
     6181 ggggtgggga aggccaccat ctatcggcgt tggtcctcca aggaggacct ggtggtggac
     6241 gcggtcgcgc gggtcttcac gaaggcggtc cgaccggaca ccggcaccgt tcgccaggac
     6301 ctcatcgaga gcgcgcgaga gctccaccgc ctcatgagct caagcctgac cggaagggtg
     6361 ttccctcgga tggcgaccga gatcgcacat cgctctcccc tcggtcgtct ctacggcgag
     6421 cggatcatcg gtcctcgccg ggccgtcttc gaggaggcgc tgcgtcgtgg gatcgagcgc
     6481 ggcgagctgc ccgaggcgat cgacctcgag ctggcgatcg atcagctcgt ggggacgctc
     6541 ctcttgcgga agctcaccgg cagattgaag cgatccgacc ccgccgtggc tgatcgggcg
     6601 gtggacatgc tcctgacggg tctcggagca gggggggcgt agtcgatggc ttcacgacgg
     6661 gcggttacgg acgttgcgga cgttgcggtg atcggggcgg gctcctctgg gctcgccgcc
     6721 ctgaaggtcc tcggcgagca gggcgtcgcc gtcgagtgct tcgagcgagg gtcggacctc
     6781 ggagggctct ggcgctacga gaacgacaac ggcctctcgg gcgcgtacgc ctcgctgcgc
     6841 acgaacgtct cgcgtcgccg gatgcagtat ccgagcttcc cgatgccgcg ttcgccgatc
     6901 gacttcccga cgcacacgga gatggccgcg tatctggatg cctacgccga tgcgtccggg
     6961 ctacgcgagc tcatcagatt cggcacgacg atcgagagga tggagcccga ccccggcggc
     7021 agatggaacc tctccctgga cgacggcgcg gtgcgcacct acaacgccgt ggtcgtcgcc
     7081 atcgggcttt tctggtctcc gaggatcccg gactatccgg gaagcttcga cggggaggcg
     7141 agccattcgc acgactaccg gacgcctggg tcctgcgccg gacgcaacgt gctcgtcgtc
     7201 ggagccggac agtcggcggc ggagatcgcc gtcgaggtgg cgggagtggc gaacaggacg
     7261 gtcatgtccg ttcggagtgc ccaccatgtg atccctcggt ggatcggcga ccgaccctac
     7321 gacgccgacg atgtcggacc actcaatcgg atgccatggc ggctcatgaa ccttctgttt
     7381 gggcggcgct cggcgcggga actcggacag gtcccgtcgt cgtggccggt cgcgagccac
     7441 cgggtgctcg agggcatccc cgtcgtctcg tcggatctcc tccccagggt gcgccggggc
     7501 gagatcgtga tcaaacccgc ggtcgaccgg ctcctcgggg atcgggtgcg gttcgtcgac
     7561 gggtcggagg aggccgtcga ccggatcgtc tacgccaccg gctaccggat cagccttccc
     7621 ttcctgtcgt cctccctggt ttcggcgagc ggccgagact tcccgctcta ccgtcggatc
     7681 ctcccgcccg acgtcggcgg gctcttcttc gtcggcttcg tggatgcgcc gggtggcctc
     7741 ctgccggtgg tggaggccca ggcggagtgg gtcgccgccg tgctcaccgg gcggctcggc
     7801 ctcccctcgc cggaacgcat gcgtcgcagg atggagcgag ccgaacggcg cacccgccag
     7861 cgcttccccg gggagggccc ccacagcacc cgctgcgacc ctcatggcta ccggcggttg
     7921 ctgcgttccg acctccgccg ggcgcggcgc aggaggccga gggctacggg gattcggtgg
     7981 agccttggcg ggagcgtccg agagaccgct cggtagggaa cccgaacgcc gagaacagct
     8041 cgtcgccgac gaagccggtg atgcgctcgc acagaccgcc ggcgacgccg aggacctgga
     8101 tggacagcgc cgtctcccgg cccgaagccc gttcgtacac agcgacggcg ggagaccgat
     8161 tggcggccgt cggttcgagc ccgagggggc ccggactcgg aagacccgaa gctcgatgaa
     8221 gcggagcacg tcatcgtgac cgcggaacca cgccgggtcg ggcggcatcg tcatctcgac
     8281 ctcgtcggcg aggatccgcg cgagcgcggg cacgtcggca cgttcgaagg cgcgcaggta
     8341 gcgatggagg atcgcgcgtt cctcaccggt cggcgcgccg atgggagctc cctcgtcgtt
     8401 ccatcgtccg atgctcgctc gcccgcgctg cagcgcgctg ttgaccgacg cggccgtcgt
     8461 gccgaggagc tccgcgacct cggtcgcgct ccactcgagc acgtctcgaa gaagcaggac
     8521 cgcgcgctgc cgcggcggca gcacgtggat cgcggcgagg aacgcgagct cgatcgtttc
     8581 ccggcgcgcg aatcgagccg cgggatcctc gagctcgtcc aggagccggt ccgggtacgg
     8641 ctcgagccag atgggctcga ggatgggttc gc
//
LOCUS       DATBRK010000051         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_293342, whole genome shotgun sequence.
ACCESSION   DATBRK010000051 DATBRK010000000
VERSION     DATBRK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293342"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..380)
                     /locus_tag="VIH70_01565"
     CDS             complement(12..380)
                     /locus_tag="VIH70_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8115082.1"
                     /translation="MQYIFLIYGDESEGSSSPEEMAKSMQEWGDYTQYLKDKGYHLAG
                     EALYPTHDATTVRVRDGKTITTDGPFAETKEQLGGFYLVDCPSLDEAIEASAKMPVGP
                     DGSVEIRPIMLFDEEGHPSS"
     gene            complement(468..1598)
                     /locus_tag="VIH70_01570"
     CDS             complement(468..1598)
                     /locus_tag="VIH70_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEY8115083.1"
                     /translation="MQTMERPIAVLQQARAPEPPEPRRASARDVFVTIVVCLCLWALL
                     AAPLLQRSAEAGPVGARRSAALALLRPLVAISDGLLLSGATSSVERALGRDPDGAPGG
                     ELILPAFELPPEGIDSKATQSPPTGAPDPAETSEPTTYEAEGEAPAAAAVLRTPTRSN
                     KLRVAVIGDSLSQGLGPAIERWMNPSVVRVLSLGRPSTGLSREDYFNWQAGMRQIVEE
                     FRPDLVFVLLGSNDAQAQISRDGTAIPVGSVRWLEGYRERAANLLGEATRAGTHVVWV
                     GIPIVKERQHWDFYRRVNDIYRDTVSADPLGTYVDTWTPFQGRDGGYAAFVRNDRGDL
                     VEVRTPDGVHFTATGYSFVARMAIRAADQAFGMPEKAVSFRV"
     gene            complement(1610..3028)
                     /locus_tag="VIH70_01575"
     CDS             complement(1610..3028)
                     /locus_tag="VIH70_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEY8115084.1"
                     /translation="MLFPTIDFAIFLAVVFALSWLLNPHRTAWKWCILAASYVFYAWW
                     DPRLVWLLALVSTLAWASGLWVERTDHERARRRRTLVSVVALLLPLAWFKYYGFFALN
                     VANAFESLGLHPPLPLLQVLLPIGISFYTFMAISYVVDVSRYQIQVSRWLDVSVYLAF
                     FPHLIAGPIVRSEELIPQIRSPRDAGRIDVSRASFLIVAGLFKKMVISSFLASQIVDP
                     VFGAPNAHGGLEVLIAIYAYAIVIYADFSAYSDIAIGVALLLGFEFPANFDRPYTAVS
                     LRDFWRRWHMTLSRWLRDYLYVPLGGSRHGQFRTAVNIMITMLLGGLWHGAGWTFVAW
                     GAFHGIGQVIGRWRTRRGEALGTVERSSPWIVARKQLVTFNLVCLGWVFFRADSIASA
                     FELLGRLGAAGTTALVTPLVLLTIATMLGVQYLPADPATRLRESFGRLGPVLQGAALA
                     MCLFVITTLGAQGVSPFIYFRF"
BASE COUNT          531 a         1100 c         1087 g          446 t
ORIGIN      
        1 ggtcgcggtc cctacgacga cgggtggccc tcctcgtcga agagcatgat cggccggatc
       61 tcgaccgagc cgtccggccc gaccggcatc ttcgcgctcg cctcgatcgc ctcgtccagg
      121 ctcgggcagt cgaccaggta gaacccgccc agctgctcct tggtctccgc gaacggtccg
      181 tcggtggtga tcgtcttgcc gtcccgaacc cgaaccgtgg tggcgtcgtg cgtcgggtag
      241 agggcctcac cggcgaggtg gtaccccttg tccttcaggt actgcgtgta gtccccccac
      301 tcctgcatgc tcttggccat ctcttcggga gaggagctcc cctccgactc gtctccgtag
      361 atcaagaaga tgtactgcac gcgttctcct tcctctcgtc ggccccgctc gtcggccgct
      421 tccaccttaa cgacgaacgg gtggggcgtg attcgacagt gggacgttca gacgcggaac
      481 gagacggcct tctcgggcat gccgaaggcc tggtcggccg cgcggatcgc catccgggcg
      541 acgaacgagt accccgtcgc cgtgaagtgg acgccgtcgg gagtgcgtac ctcgacgagg
      601 tcaccgcgat cgttgcggac gaacgccgcg tacccgccgt cccggccctg gaacggggtc
      661 caggtgtcga cgtaggtgcc aagcggatcc gcggagacgg tgtcgcggta gatgtcgttc
      721 acgcggcggt agaagtccca gtgttgacgc tccttgacga tggggatccc gacccacacg
      781 acgtgcgtcc ccgcgcgggt cgcctccccc aggaggtttg cagcccgttc gcggtacccc
      841 tcgagccacc ggacgctccc cacgggtatc gcggtgccgt cgcgcgagat ctgcgcctga
      901 gcgtcgttgc tgccgagcag cacgaacacc aggtccggcc ggaactcctc gacgatctgg
      961 cgcatgcccg cctgccagtt gaagtagtcc tcgcgcgaga gcccggtcga cggccggccc
     1021 agggacagca cccgcacgac ggaggggttc atccatcgct cgatcgccgg gccgagaccc
     1081 tgggagagcg agtcgccgat cacggccacg cggagcttgt tcgagcgggt cggggtgcgc
     1141 aggacggcgg cggccgcagg cgcttccccc tcggcctcgt acgtggtcgg ctccgacgtc
     1201 tcggctggat cgggcgcgcc cgtgggaggg gactgcgtgg ccttggagtc gatcccctcg
     1261 ggcgggagct cgaacgcggg caggatcagc tcgccgccgg gcgcgccgtc gggatcgcgc
     1321 ccgagcgcgc gctcgaccga gctcgtcgcc ccggacagga gcagcccgtc ggagatcgcg
     1381 acgagcggcc gcaggagcgc gagcgccgcc gagcgccgcg cgccgacggg ccccgcctcg
     1441 gcgctccgct gcagcagcgg cgccgcgagc agcgcccaca agcacaggca gacgacgatc
     1501 gtcacgaaga cgtcccgagc cgaggcgcgc cgtggctcgg gtggttccgg cgccctcgct
     1561 tgctggagga ccgcgatcgg ccgctccatc gtctgcatca gccgtctcct cagaaccgga
     1621 agtagatgaa cgggctcacg ccctgggccc cgagcgtcgt gatcacgaac aggcacatcg
     1681 cgagcgcggc gccctggagc accggcccga gccgtccgaa gctctcgcgc agccgtgttg
     1741 cgggatcggc cgggaggtac tggacgccga gcatcgtcgc gatcgtcagg agaaccaggg
     1801 gcgtgacgag cgccgtggtg cccgccgcgc cgagccggcc caggagttcg aacgcgctcg
     1861 cgatcgagtc cgcgcggaag aacacccaac cgaggcagac gaggttgaac gtcacgagct
     1921 gcttgcgcgc gacgatccac ggcgacgacc gttcgacggt cccgagggcc tccccgcgtc
     1981 gcgtccgcca acgcccgatc acctgcccga tcccgtggaa cgctccccac gcgacgaacg
     2041 tccacccggc cccgtgccac aaccccccga gcagcatcgt gatcatgatg ttcaccgccg
     2101 tgcggaactg gccgtgccgg ctcccgccga gcgggacgta caggtagtcg cgcaaccacc
     2161 gggacagcgt catgtgccaa cggcgccaga agtcccggag cgagacagcc gtgtacgggc
     2221 ggtcgaaatt cgcggggaac tcgaacccga gcagcagggc gacgccgatc gcgatatcgc
     2281 tgtaggcgct gaagtcggcg tagatcacga tcgcgtacgc gtagatcgcg atcaggacct
     2341 cgagcccgcc gtgcgcgttc ggcgcgccga acacggggtc cacgatctgg ctcgcgagga
     2401 agctcgagat caccatcttc ttgaacagcc cggcgacgat caggaacgac gcgcgcgaca
     2461 catcgatcct gcccgcgtcc cgggggctgc ggatctgcgg gatcagctcc tcgctgcgga
     2521 cgatcggacc ggcgatcagg tgcgggaaga acgccaggta caccgagacg tcgagccacc
     2581 gcgacacctg gatctgatac cgcgagacgt cgacgacgta gctgatcgcc atgaacgtgt
     2641 agaaggagat cccgatcgga agcaggacct ggagcagggg cagcggcggg tggagcccga
     2701 gcgattcgaa cgcgttcgcc acgttcagcg cgaagaaccc gtagtacttg aaccacgcga
     2761 gcgggagcag gagcgcaacg acgctgacga gcgtccggcg acggcgcgca cgctcgtggt
     2821 cggtccgttc cacccacagc cccgacgccc acgcgagggt gctgacgagc gcgaggagcc
     2881 agacgagccg cgggtcccac cacgcgtaga agacgtagct ggcggccagg atgcaccatt
     2941 tccaggcggt tcggtgcggg ttcagcagcc aggacagggc gaacacgacc gccaggaaga
     3001 tcgcgaagtc gatcgtcgga aacagcatcg tcggttatgg cggccgcgcg gccgcccctc
     3061 gctcagaccg tgcctcgggc catggggtac gggacgacgc taggctagcg aagcgccatg
     3121 gccgaatcac cggatcccct ggatttcttg gacctcgatc acct
//
LOCUS       DATBRK010000052         3580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1593420, whole genome shotgun sequence.
ACCESSION   DATBRK010000052 DATBRK010000000
VERSION     DATBRK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593420"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VIH70_01580"
     CDS             <1..271
                     /locus_tag="VIH70_01580"
                     /inference="COORDINATES: protein motif:HMM:NF044818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115085.1"
                     /translation="TETVEFVGFVSIRDDTPADEFLLARYVVTCCVADATIAEVRVVN
                     VTPGQFANEDWVQVTGTIFPIGREVIVDASDIIAVERPDHPYLTP"
     gene            complement(268..699)
                     /locus_tag="VIH70_01585"
     CDS             complement(268..699)
                     /locus_tag="VIH70_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY8115086.1"
                     /translation="MNVELHDAVELRLRAIGQRYTPKRRALVDALVRASQPVAVPDLA
                     RGRGLPQSSLYRNLAVLEQAGAVHRVMTHEEFGRYELAEDLTQHHHHLVCRNCGTVED
                     VTVAPSLERQVQRSLVDVEDRTGFSAVSHRLDLFGTCRNCS"
     gene            complement(696..1550)
                     /locus_tag="VIH70_01590"
     CDS             complement(696..1550)
                     /locus_tag="VIH70_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY8115087.1"
                     /translation="MPWPVDALPYPFELEFMQRALVAGIVVGIFAPMIGTFVVQKRMS
                     LIGDGIGHLAFAGVGVGLVASVSPIWAALAVAVAGALGIEWLRSRRTASGDLALALFF
                     YSGIAAGVVLVSLGGGFDANILSFLFGQALTVNDQEIAIIVGLGAAIVAAMLVLRRGL
                     FAVVTDEDWSRVVGLPVGFLNTVLAVIVATSVVAAMRIVGLLLVAAMMVLPVASGQLL
                     ARSFRGTMWWSVGIGVASVVAGLASSRFWGVAPGGAIVLISAAVFVVVTLLKRATLPK
                     LQAEGAGL"
     gene            complement(1550..2329)
                     /locus_tag="VIH70_01595"
     CDS             complement(1550..2329)
                     /locus_tag="VIH70_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEY8115088.1"
                     /translation="MTAPDVSQRLAAATQIEARGVSFAYGRQAVLEDVDLVVRAGEFV
                     ALVGPNGSGKSTLLRVLLGSLEPSSGTVRLFGEPPSRFADRERIGYVPQRPVLDSEVP
                     ATVEEIVTAGRLARRGWWRPLHRDDHEQVGHALDSVGLGDLAKRPLNELSGGQQQRAF
                     IARAFAGEPELLVLDEPIAGVDAESQRRFRDSITHLIQEHGAGVLLVSHELSAVAPEL
                     DRVVVLKRRVVFDGAPAELAASGVSLGVHPEDLPLWLEELR"
     gene            complement(2316..3206)
                     /locus_tag="VIH70_01600"
     CDS             complement(2316..3206)
                     /locus_tag="VIH70_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY8115089.1"
                     /translation="MCSCLLGVLLVACNSSTDAGESGTTSVVASFFPLAEAAQQVGGD
                     LISVENLTPPGVEPHDLELAPDDIEAIATADVIVYLGGGFQPAVEDAVSQAEDAVTVD
                     ALEAVDTNEAPPSEAEEGLTVDPHVWLDPARYEEVVRTVADALSESDPANESTYDANA
                     DAYIAKIAALDDDFRAGLADCQRTTIVTSHEAFAYLAESYGLTEVGITGLSPEAEPSA
                     QRLAELKDLVEKEGITTIFAEELVSPKVAETLADEAGITVEVLDPLESLTDEQVAAGE
                     DYVSVMRENLAILRGALDCS"
     gene            complement(3362..>3580)
                     /locus_tag="VIH70_01605"
     CDS             complement(3362..>3580)
                     /locus_tag="VIH70_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115090.1"
                     /translation="SGALAERVFAEKLQRAGFEDVWVGGHQPYGIEDAARYPLFTAEL
                     IEIMKRTISPERRDHVATGVIVKARKPR"
BASE COUNT          592 a         1294 c         1201 g          493 t
ORIGIN      
        1 gaccgagacc gtcgagttcg tcgggttcgt ctcgatccgc gacgacaccc cggccgacga
       61 gttcctgctg gcgcggtacg tcgtgacctg ctgcgtcgcc gacgccacga tcgccgaggt
      121 gcgggtggtg aacgtgacgc cggggcagtt cgcgaacgag gactgggtgc aggtgaccgg
      181 gacgatcttc ccgatcgggc gggaggtcat cgtcgatgcc tccgacatca tcgcggtcga
      241 gcggcccgac cacccgtacc tgactcccta gctgcagttc cggcaggtcc cgaacagatc
      301 gagccgatgg ctgaccgccg agaagccggt ccggtcttcg acatcgacga ggctgcgctg
      361 gacctgtcgt tcgaggctgg gcgcgaccgt gacgtcctcg acggtgccgc agttccggca
      421 caccaggtgg tggtggtgct gcgtgaggtc ctcggcgagc tcgtagcggc cgaactcctc
      481 gtgcgtcatc acccgatgga cggccccggc ctgctcgagg accgcgaggt tccgatacag
      541 cgaactctgc gggaggcctc gtccgcgggc cagatcgggg acggccacgg gttggctggc
      601 gcgcaccaat gcgtcgacca gggctcggcg cttgggggtg taccgctggc cgatcgcccg
      661 cagacggagc tcgacggcgt cgtggagctc cacgttcaca gaccggcccc ctccgcctgg
      721 agcttgggca acgtagcacg cttgagcagc gtgacgacca cgaacacggc ggcagagatc
      781 aggacgatcg cgccgcccgg ggcgacaccc cagaacctgg acgaggccag cccggcaacg
      841 acggacgcga cgccgatccc gacgctccac cacatcgttc ctcggaacga gcgggcgagc
      901 agctggccgc tcgcgacggg caggaccatc atggccgcga ccagcaggag accgacgatc
      961 cgcatcgcgg ccaccacgga ggtcgccacg atgaccgcga ggaccgtgtt gaggaacccg
     1021 acggggagtc ccacgacgcg ggaccagtcc tcgtccgtga cgaccgcgaa cagcccgcgc
     1081 ctgagcacga gcatcgccgc gacgatcgcg gctccgagcc cgacgatgat cgcgatctcc
     1141 tgatcgttca cggtgagggc ctgcccgaac aggaacgaca ggatgttcgc gtcgaagccc
     1201 ccgcccaggc tcaccaggac gacccccgcg gcgatacccg agtagaagaa cagcgcgagc
     1261 gccaggtcgc cggaggccgt gcggcgcgag cggagccact cgatacccaa ggcgcccgcg
     1321 accgccacgg cgagcgccgc ccagatcggc gaaacgcttg cgaccagccc gacccccacg
     1381 ccggcgaagg cgagatgccc gatcccgtcg ccgatcaacg acatccgctt ctgcacgacg
     1441 aacgtgccga tcatcggcgc gaagatgccg accacgatgc ccgcgaccag cgcacgctgc
     1501 atgaactcga gctcgaacgg atacggcaag gcgtcgacgg gccacggcat cagcggagct
     1561 cctccagcca gagggggagg tcctccgggt ggacgccgag gctcacgccc gaggccgcga
     1621 gctcggctgg cgcgccgtcg aacacgaccc gtcgcttcag gaccacgacg cgatcgagct
     1681 cgggcgcgac cgccgacagc tcgtgcgaga ccaggagcac gccggcgccg tgctcctgga
     1741 tcaggtgcgt gatcgagtca cggaaccggc gctgggactc ggcgtccacc ccggcgatcg
     1801 gctcgtccag aacgagcagc tcgggttcgc cggcgaacgc tcgggcgatg aacgcgcgct
     1861 gctgctgccc acccgagagc tcgttcagcg gtcgcttggc caggtcgccg agcccgaccg
     1921 agtccagagc gtgccccacc tgctcgtgat cgtcccggtg gagcgggcgc caccaacccc
     1981 gccgtgcgag gcggccggcc gtgacgatct cttccaccgt ggcgggcacc tcggagtcca
     2041 ggacaggacg ctgcggcacg tagccgatcc gctcccgatc cgcgaaccgc gacggcggct
     2101 cgccgaacag cctgacggtc cccgacgacg gctcgagcga gccgaggagc acccggagca
     2161 gggtggactt cccggagccg ttcggcccca cgagggccac gaactcgccg gcgcggacca
     2221 cgaggtcgac gtcctcgagc accgcctggc ggccgtacgc gaacgagacg cccctcgcct
     2281 cgatctgggt cgcggcggcg aggcgttggg agacgtcagg agcagtcaag cgcaccccgc
     2341 aggatggcca ggttctctcg catcaccgaa acgtagtcct ccccggccgc cacctgttcg
     2401 tccgtcaggc tctcgagcgg atcgagcacc tcgaccgtga tgccggcctc gtcggcgagc
     2461 gtctccgcca ccttgggcga gaccagctcc tcggcgaaga tcgtcgtgat gccctccttt
     2521 tcgacgaggt ccttgagctc ggccagccgc tgggccgagg gctccgcctc gggcgacagg
     2581 cccgtgatcc ccacctcggt cagcccgtag gactcggcca gataggcgaa cgcctcgtgg
     2641 ctcgtcacga tcgtcgtgcg ctggcagtcc gccaggcccg cgcggaagtc gtcgtcgagc
     2701 gccgcgatct tcgcgatgta cgcgtcggca ttcgcgtcat aggtggactc gttcgctggg
     2761 tcggactccg agagcgcgtc ggccacggtc cgaacgacct cctcgtaccg ggcgggatcg
     2821 agccacacgt gcgggtcgac cgtcagaccc tcctccgcct cactcggcgg ggcctcgttc
     2881 gtgtcgacgg cctccagggc gtccacggtc acggcgtcct cggcctggga gaccgcgtcc
     2941 tcgaccgcgg gttggaaccc gccgcccagg tagacgatca cgtcggccgt ggcgatcgcc
     3001 tcgatgtcgt cgggggcgag ctccaggtcg tgcggctcca cgcccggcgg cgtcaggttc
     3061 tccaccgaga tcagatcccc accgacctgc tgcgccgcct ccgcgagggg aaagaagctc
     3121 gcgaccacgg aggtagtccc gctctcgccc gcgtccgtcg atgagttgca agcgacgagc
     3181 aggaccccga gcagacacga gcacaccgct ggtccgatcc tccccatgcc aaccccttcc
     3241 ttgaaatgag aatgattccc acactcaaat caggaacgaa gggtacggtg aggcccacgt
     3301 ccgcgtcaat cgatcgatcg gggtcacgtc gtgggcgccg tcgatacgcc cacggcgagc
     3361 cctaccgtgg cttgcgggcc ttcacgatca cgccggtggc cacgtggtcc cggcgttcgg
     3421 gcgagatcgt tcgcttcatg atctcgatca gctccgcggt gaacagcggg tacctcgccg
     3481 catcctcgat cccgtacggc tggtgaccgc cgacccagac gtcctcgaaa cccgctctct
     3541 ggagcttctc ggcgaagaca cgctccgcca gggcgcccga
//
LOCUS       DATBRK010000053         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_727556, whole genome shotgun sequence.
ACCESSION   DATBRK010000053 DATBRK010000000
VERSION     DATBRK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727556"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VIH70_01610"
     CDS             complement(<1..564)
                     /locus_tag="VIH70_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115091.1"
                     /translation="MIGGRRSSRRTPRLRSVALAGLILALLAGCTSSDGGSTGSTGAS
                     GSTDAGTQTAAPAEAIPLDVFVYNVEYGGDESTDAVIRGVDADIVGVLESYNRLPEIA
                     ANTGYPYYNVSLQLLSKYPIHEPSGAEGRYALIEVRPGQVVAFFNIHLDYVKYGPKLL
                     RNGASVEEVLASENEVRTSVLDEPLRLM"
     gene            complement(561..>1743)
                     /locus_tag="VIH70_01615"
     CDS             complement(561..>1743)
                     /locus_tag="VIH70_01615"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY8115092.1"
                     /translation="IRGAAPVREVIERCAAIAMPPEAPTGARDPVELPGGVAGNVGRG
                     EGVRRGVGFAIGYKNHAYSAGFDDSSEARVTLSAGPEGPVAEIHCAAAEVGQGVHTIL
                     AQIARTVLGIERVILHTSDTLVGSAGSTSASRQTVVSGGAVKAACEAVLEDLFERARR
                     DARLIASDLGVEDGFVIADGERVAPVEELLAQPIECTRVWRHARTQPLDDRGQGDVHV
                     GFLFGAQRAVVDVDEELGLVRVVQLATAQDVGFAINPQSVIGQIEGGSAQGLGLALME
                     EIQLKDGEIANASFTDYLLPTMLDMPRVEVELIEEPEPGTPFGAKGVGEPSNVVAPAA
                     IVAALRAALGRELPRIPVRPDDLVGLREPAAIPPWPAFPERPAREPSPASAGVPRTGE
                     P"
BASE COUNT          252 a          643 c          602 g          246 t
ORIGIN      
        1 catgagccgg agcggctcgt ccagcaccga ggtgcgcacc tcgttctccg acgcgagcac
       61 ctcctcgacc gaggcgccgt tgcgcaggag cttcggcccg tatttcacgt aatccagatg
      121 gatgttgaag aacgcgacga cctgacccgg ccggacctcg atcagcgcgt agcgaccctc
      181 ggcgcccgac ggttcgtgga tcgggtactt cgacaggagc tgcaggctca cgttgtagta
      241 cgggtacccg gtgttcgcgg cgatctcggg caggcggttg tacgactcga gcacgccgac
      301 gatgtccgcg tccacgccac ggatgaccgc atcggtggac tcgtcgccgc cgtactcgac
      361 gttgtagacg aagacatcga gcgggatcgc ttcggccggc gccgctgttt gggtcccggc
      421 atctgtcgaa ccgctcgcgc ccgttgaacc ggtcgagcca ccgtcgctgg acgtgcaccc
      481 ggcaagcaac gcgaggatga gacccgcgag cgcgacggat cggagccgag gggttcggcg
      541 tgacgagcgc cgcccgccga tcacggttca cctgttctag gtacgcccgc cgacgcgggc
      601 gagggctcgc gcgcgggtcg ttcggggaag gcgggccagg gggggatcgc ggcgggttcg
      661 cgcaggccca ccaggtcgtc ggggcggacg gggatgcgag ggagctcgcg cccgagggcg
      721 gcccggagcg ccgcaacgat cgcggccggc gccacgacgt tggagggctc gccgacgccc
      781 ttggcgccga acggcgtacc gggctctggc tcctcgatca gctcgacctc gaccctcggc
      841 atgtccagca tcgtcggcaa caggtagtcg gtgaaggacg cgttcgcgat ctcgccgtcc
      901 ttcagctgga tctcctccat cagcgccagg ccgagcccct gcgccgaacc cccctcgatc
      961 tgcccgatca cgctctgcgg gttgatcgcg aacccgacgt cctgcgcggt cgcgagctgt
     1021 accacgcgca ccaggccgag ctcctcgtcc acgtcgacca cggcccgctg tgcgccgaac
     1081 aggaacccga cgtgcacgtc gccctgcccg cgatcgtcga gcggctgggt ccgggcgtgg
     1141 cgccacaccc gcgtgcactc gatcggctgg gcgagcagct cctccacggg cgccacccgc
     1201 tcgccgtccg cgatcacgaa gccgtcctcg acgccgagat cggaggcgat cagcctcgcg
     1261 tcgcgccggg cgcgttcgaa caggtcctcg agcaccgcct cgcacgccgc ctttaccgcg
     1321 ccgcccgaga ccacggtctg ccgcgaagcg gaggtcgacc cggccgagcc gacgagcgtg
     1381 tccgacgtgt gcaggatcac gcgctcgatc ccgagcaccg tccgcgcgat ctgcgcgagg
     1441 atcgtatgga cgccctgtcc cacttcggct gcggcgcaat ggatctcggc cacagggccc
     1501 tcaggtcccg ccgagagcgt gacccgcgcc tccgaggagt cgtcgaaacc ggcgctgtag
     1561 gcgtggttct tgtacccgat cgcgaacccg accccgcgac gaacgccctc gccgcgcccg
     1621 acgttgccgg ccacgccgcc ggggagctcg accgggtcgc gcgcgccggt gggcgcctcg
     1681 ggcggcatcg cgatcgcggc gcagcgctcg atcacctcgc gcaccggggc cgcgccccgg
     1741 atc
//
LOCUS       DATBRK010000054        12650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_151462, whole genome shotgun sequence.
ACCESSION   DATBRK010000054 DATBRK010000000
VERSION     DATBRK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12650
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151462"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VIH70_01620"
     CDS             complement(<1..518)
                     /locus_tag="VIH70_01620"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY8115093.1"
                     /translation="MSPAPNRVLEVVPDAEAVARRAADVLVAAAREAIAARGRFTFAL
                     SGGRSPWTMFRDLEDEEMPWEEVGIWQVDERVAPDGDRDRNLTGIEPVIPSAADLRPM
                     PVTEPDLEAAAGRYAASLPEAFELVHLGMGDDGHTASLVPGDPVLHVTDRDVAVTGEY
                     RGLRRMTVTYPVL"
     gene            complement(515..955)
                     /locus_tag="VIH70_01625"
     CDS             complement(515..955)
                     /locus_tag="VIH70_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY8115094.1"
                     /translation="MGEPDLSRLLLEASRVLGAEIVAGLQERGFPDARPGHAALFMHI
                     DRRWGTRLTDLARRARMTKQGMMLLVDDLETRGYVRRVPDADDSRAKMVRLTARGRRF
                     VAEARRAVAAVEARARRELGERRYEALRDSLEFLVGGGEGPEEA"
     gene            1026..1535
                     /locus_tag="VIH70_01630"
     CDS             1026..1535
                     /locus_tag="VIH70_01630"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8115095.1"
                     /translation="MAIEVRPARPEEYEETGRVTALAYREFADGDSWDRYLQLIADVG
                     ARADIALVLAAFEDGRILGSATLELDQRIEDDDPVLPPEQAEIRMLGVDPDRRGCGVA
                     KALMDACFAWAARAGKTRMLLHTTQRMKVAQAMYEAMGFERLPDRTFPDGFVLLTYEK
                     AIGPETREP"
     gene            complement(1532..3505)
                     /locus_tag="VIH70_01635"
     CDS             complement(1532..3505)
                     /locus_tag="VIH70_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8115096.1"
                     /translation="MVMHGRYMRAPAQEDDRPIRAKTVRRVIGTFKPYRRKVAWVGLA
                     IVVTSGLGVVNPLLIKWIFDNGLFGNPPNLCAGQPCPQLDTVYIGVALMIAIPIVTGV
                     IGVGQTYLANWVGLRVMQDLRNALYSHLQFMPLRFFTTTRTGEIQSRIANDVGGVQAV
                     FTDTVSNILSNIVVIASTLVAMLLLSWQLTVLSLFMLPFFLWLTVKVGRARREVATST
                     AKTYADLTAVTEETLSVSGILLSKSFGRQRHEIGRFRDENERLTGLQMRQTMIGRSFF
                     AIVGTFFSITPALAYLVAAWVISRDPTGSTITAGTIVAFTTLQSRLFFPIGSMLQVST
                     EVHSSMALFDRIFEYLDLDHEIEDSPDAVAIPPDRVLGRVELDGVWFRYETPAGERAG
                     PVSPSDLELANEAPREWTLEDVSLTIEPGQLAALVGPSGAGKTTMTYLVPRLYDVQRG
                     AVKIDGVDVRNLKLESLGDLIGVVTQETYLFHTTIRRNLLYGRPDATEDELDAAARAA
                     NIYERIAELPEGYDTIVGERGYKLSGGEKQRLAIARVILKDPRILILDEATSSLDTTS
                     ERLVQAALVPLMHGRTTIAIAHRLSTILSADVIFVADRGRIVERGTHAELLRRGGVYA
                     RLYEQQFRGGLVEAVTEDGVIMATGEVVRTGSD"
     gene            complement(3583..4263)
                     /locus_tag="VIH70_01640"
     CDS             complement(3583..4263)
                     /locus_tag="VIH70_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY8115097.1"
                     /translation="MSARDLERITGEIVGCRSCPRLVEWRERVAREKTVRFADQTYWG
                     RPVPGFGDPAAGILVVGLAPAAHGGNRTGRIFTGDRSGDFLFASLHRFGLSNQPTSVS
                     RADGLRLIRVYLSAVNRCAPPDNRPTPAERDTCLPFLEREIAALERLRVLVALGAFAW
                     DGALRALSALGHPTRPRPRFGHAAEAKVEPFVLLGSYHPSQQNTFTGKLTPQMLDAVF
                     ERAAELAG"
     gene            complement(4260..4661)
                     /locus_tag="VIH70_01645"
     CDS             complement(4260..4661)
                     /locus_tag="VIH70_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115098.1"
                     /translation="MSPRRRRLPEHLEGPARAFDDLLPPLGRAEAALTGSVPGTRLPG
                     RPLAETLSEFEDGLREVREGMDAWRSTGVEPEWEACSRGLDEALALADRVRTQGAEPE
                     GFEGLIGLIGDLLAPLDAFERASERLRELRG"
     gene            complement(4658..5443)
                     /locus_tag="VIH70_01650"
     CDS             complement(4658..5443)
                     /locus_tag="VIH70_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY8115099.1"
                     /translation="MARGHRFPNDAFPFVVAHRGASSTRPENTIPSFEEAIRLGAGIV
                     EFDVRLSRDGVPVVIHDPAVDRTTGGSGLVHELSAADLSSLNAGTEAEPTPVPTLAEV
                     LRCVSGRAAIAAEVKNIPGEPAFEPTREPIVEAIHAELERQGFDGPVLVISFNPSSIA
                     ASKLVTPDVPTGFLTTDLVPPREALAYAASAGHDMVLPGTRAAIPAGPSFVEQVHAAG
                     LRVGTWTVDDPETVRMLLDRGFDAVASNDPEMALAVLADHVRS"
     gene            complement(5444..6115)
                     /gene="nth"
                     /locus_tag="VIH70_01655"
     CDS             complement(5444..6115)
                     /gene="nth"
                     /locus_tag="VIH70_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY8115100.1"
                     /translation="MSAGKAPKGLGRDAPIEERAPTIIRILSEAYPDAKVALNFSNPL
                     EMLVATILSAQCTDEKVNEVTATLFRKYRGAEDYLKVPEDELRADIKPTGFFNQKATS
                     IREACRRIVEVYGGQVPDTMEDLITLRGVARKTANIVLGNAFGTVEGIAVDTHVKRLA
                     NRLGFSQESDPDKIEQDLMRRIPQERWFDFTYVLIDHGRALCVARKPKCEVCPVNHLC
                     PSSLV"
     gene            complement(6112..6852)
                     /locus_tag="VIH70_01660"
     CDS             complement(6112..6852)
                     /locus_tag="VIH70_01660"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY8115101.1"
                     /translation="MSEISLGFLGSGEFDPWSEPVERWLLARSRTLDGPVLVCATASA
                     HEGEASYDGWANKGLEHYGSLGIAAEVLPLKTREDAHRDEVIAKLEGASAIFFSGGNP
                     ARLARVMVGTPFWAALQAAIRAGLPYAGCSAGVACLTEKTYDSDSQDFDSIWAPGIGY
                     VRDTLFGPHWDIVDTWIPGATEFIAGSLEPGGTFIGLDEDTAMVGDGRSWEVIGRAKV
                     HVMREGEWTRYAEGDEIELSLPLADEAA"
     gene            complement(6849..7559)
                     /locus_tag="VIH70_01665"
     CDS             complement(6849..7559)
                     /locus_tag="VIH70_01665"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY8115102.1"
                     /translation="MDELGNILGVWAHPDDEAYLTAGIMARAVRNGSRVVCVTATRGE
                     GGSMDEEKWPPESMGEVRTKELERSLQILGVDEHVWLDMRDVDMDTGLPDEGYDRIRE
                     IVADVQPDTILTFGPDGMTGHAAHKDASAWATKALHEVGKPGSRVLYATVTPEFASEI
                     LPVWEPFNVFRPGTPPITARDELSVYYELPEDILALKVDAINAHVSQVEAILEAVGPD
                     LWWRQMSTEAFGPGEERT"
     gene            complement(7752..7940)
                     /locus_tag="VIH70_01670"
     CDS             complement(7752..7940)
                     /locus_tag="VIH70_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115103.1"
                     /translation="MTKGSRPGNLPSPSRTLERGRVCAHEGCRTTLSVYNEGAFCWQH
                     ADVVFPNYRGKRLRNDRA"
     gene            complement(8026..8250)
                     /locus_tag="VIH70_01675"
     CDS             complement(8026..8250)
                     /locus_tag="VIH70_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY8115104.1"
                     /translation="MLKQIELYLDGELVASMRVEIEQHLGGCDPCSGHAAFQRRLKEV
                     LRSKCGCDQVPPELVDRLRALLERSHAHPS"
     gene            complement(8261..8938)
                     /locus_tag="VIH70_01680"
     CDS             complement(8261..8938)
                     /locus_tag="VIH70_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007322071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8115105.1"
                     /translation="MRDTEGSLGERAGNETRPSKRAREREGGSAVARSPVAPPEDPAL
                     RERFEREVLPLLPNLYGAALRMTRNPQDAEDLVQETYLRAYRGFSGFREGTNLRAWVY
                     RILTNTFINSYRKKQREPLTVPDDDTPDWYLYDKLGASNVEASAESEVLEKLPDEDVQ
                     RALEDLPEGFRMAVLLADVEGFSYKEIAEIMDVPIGTVMSRLHRGRKALQKALWETVR
                     ERGLVTD"
     gene            9002..11014
                     /gene="ftsH"
                     /locus_tag="VIH70_01685"
     CDS             9002..11014
                     /gene="ftsH"
                     /locus_tag="VIH70_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY8115106.1"
                     /translation="MANNDGKRGRWQGFDLGGGAPSGPRRVRSSPLVWAVVFVLILIA
                     FQFLTSARPHTIDYSNFLERVKDGQIVGTLEISQTSVSGRYEDSTGTVDFNTTIPPIL
                     QGTSTLTNQLDAAGISYTGETPGGLQTILLAWVVPILLFGFVWFFLVRRMSGAQAGAA
                     LNLGRNKVKIYDRKEMKTTFADVAGVDEAKEELKEIVEFLRNPKKYQRLGGRIPKGVL
                     LLGPPGCGKTLLARAVAGEANVPFFFMSGSEFVEMFVGLGAARVRELFQQAKEKAPAL
                     VFLDEIDTIGKGRGGALGAGFGAHDEREQTLNQLLVEMDGFDSQKGVIIMAATNRPDV
                     LDPALVRPGRFDRQVVVDQPDLRGREEILQVHVRGVALDRQVDLRVLASRTPGFTGAD
                     LANVVNEAALLAARREREAVTMVELEEAIDRTVAGLERKSRVMSVKDKERVAIHEMGH
                     ALVALTAETADPVHRVSIIPRGAAALGMTMQRPLEDRYLLTEPELKDRLAVLLGGRTA
                     EEILFDLVVSTGAQNDLERATEISRAMVTEYGMSPKIGPVSFGNDGFRQGAGFLYPGG
                     SPELSDDLAALIDEETARLVNEAHERATEVLTRYRGLLEKLSKILIVIEVIDGPDLQA
                     YFDGSKPIPTPEELEDQIHRNAGTATPGTAAPGSGGPDIVLQPRPS"
     gene            complement(11011..11916)
                     /locus_tag="VIH70_01690"
     CDS             complement(11011..11916)
                     /locus_tag="VIH70_01690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8115107.1"
                     /translation="MTEVPDEVRALAAERDERRRAKDFAAADALRDRIARLGFSVVDG
                     PDGPSLEPLAVAELHRIAAHDVESLLDEPSTADASVHWVVEGWPEDVARGIAGFRANE
                     GGRDVRYVVADLTETDPSTYGAGVEVIALETGTGWGAALNAGLRRSRGRSVLVMDGSV
                     EPTGDVFAPLEAALEDPSVGVCGPFGISTEDLREFHESDGPEVDAIEGYMMAFRRDVI
                     RDVGLFDEKFRWYRTADIEYSFRIKDAGLRALRVDAPVVKHEHRMYHTTPEAERDRLS
                     KRNYYRFLERFRDRYDLLVRRPTDG"
     gene            complement(11939..12358)
                     /locus_tag="VIH70_01695"
     CDS             complement(11939..12358)
                     /locus_tag="VIH70_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8115108.1"
                     /translation="MAAPSVANNLASVPIFSGCSKRELGIIARASKEVSHKEGTVIAR
                     EGERGIGLFLILDGQCKVTIGGKTKARLGAGDFFGEVALLDGGPRTATVTAVSLVRLV
                     GITGWVFRGLLMEHPTIALKTLEAVAGRLRSVSKEPV"
BASE COUNT         1893 a         4489 c         4333 g         1935 t
ORIGIN      
        1 aggacgggat aggtcaccgt catccgccgg agaccccggt actcgcccgt caccgccaca
       61 tcgcggtccg tcacgtggag gaccgggtcg cccggcacga gcgacgcggt atggccgtcg
      121 tcgcccatcc cgaggtgcac gagctcgaac gcctccggca gggacgccgc gtaacggccg
      181 gccgccgcct cgaggtcggg ttcggtcacg ggcatcggcc gcaggtccgc cgcggagggg
      241 atcaccggct cgatccccgt gaggttccgg tcgcggtcgc cgtcgggggc cacgcgctca
      301 tccacctgcc agatgccgac ctcttcccac ggcatctcct cgtcctccag gtctcggaac
      361 atcgtccacg gagaacggcc gccgctcagg gcgaaggtga accggccgcg cgccgcgatc
      421 gcctcgcgcg ccgccgcgac caggacgtcg gccgcgcgcc gcgccacggc ctcggcgtcg
      481 ggcacgacct cgaggacgcg gttcggggcg gggctcatgc ctcctcgggc ccttctcccc
      541 cgcccacgag gaactcgagc gagtcgcgga gcgcctcgta tcggcgctcg ccgagctcac
      601 gccgcgcgcg ggcttcgacc gcggcgacgg ccctgcgagc ctccgccacg aaccgccggc
      661 cccgagccgt gagccgcacc atcttcgctc gcgaatcgtc cgcgtcgggc acgcgtcgta
      721 cgtacccacg cgtctcgagg tcgtccacga gcagcatcat cccctgtttg gtcatgcgcg
      781 cgcggcgcgc gagatccgtc agacgcgtcc cccaccgccg atcgatgtgc atgaacagcg
      841 ccgcgtgtcc cggacgcgcg tcggggaacc cacgttcctg gaggcccgcg acgatctcgg
      901 cgccgagcac gcgcgaggcc tcgagcagca atcgggacag gtccggctcg cccacgcggg
      961 catcgtcacc cgcgatccgg cgaacgtcaa ccacattgac cgccactcgg cggtacgctc
     1021 ccacgatggc gatcgaggtt cgtcccgcgc gtccggagga gtacgaggag acgggccggg
     1081 tgacggcgct cgcctatcgc gagttcgccg acggcgactc gtgggaccgg tacctccagc
     1141 tgatcgcgga cgtcggcgcc cgcgcggaca tcgccctcgt ccttgcggcg ttcgaggacg
     1201 gccggatcct cgggagcgcc acgctggagc tcgaccagcg gatcgaggac gacgacccgg
     1261 tccttccgcc cgagcaggcc gagatcagga tgctcggcgt cgaccccgat cggcgagggt
     1321 gtggcgtggc gaaggcgctc atggacgcct gcttcgcatg ggcggcgagg gccggcaaga
     1381 cacggatgct cctgcacacc acgcaacgga tgaaggtcgc gcaggcgatg tacgaggcga
     1441 tggggttcga gcggctgccc gaccgaacgt tccccgacgg cttcgtgctg ctgacgtacg
     1501 agaaggccat cggtcccgag acgcgcgagc cctagtcgct cccggtccgg acgacctcgc
     1561 ccgtcgccat gatgacgccg tcctcggtga ccgcctcgac caggccgccg cggaactgct
     1621 gctcgtacag tcgcgcgtac acgcctccgc gccgaagcag ctcggcgtgc gtcccgcgtt
     1681 ccacgatgcg gccacgatcg gccacgaaga tcacgtccgc cgacaggatc gtcgagagcc
     1741 ggtgggcgat cgcgatcgtc gtccgaccgt gcatcagcgg caccagcgcg gcctgcacca
     1801 gccgttccga cgtcgtgtcc agcgacgagg tggcctcgtc caggatcagg atcctcgggt
     1861 ccttcaggat cacgcgggcg atcgcgagcc gttgcttctc gccgcccgag agcttgtacc
     1921 cccgctcgcc gacgatcgtg tcgtagccct ccgggagctc ggcgatccgc tcgtagatgt
     1981 tcgcggcgcg cgcggccgcg tcgagctcgt cctccgtggc gtccggccgc ccgtacagga
     2041 ggttcctgcg gatcgtggtg tggaacaggt aggtctcctg cgtgacgacg ccgatcaggt
     2101 cgccgagcga ctcgagcttg aggttgcgca cgtcgacccc gtcgatcttg accgcgccgc
     2161 gctgcacgtc gtacaggcga ggtacgaggt aggtcatcgt ggtcttgccg gctcccgacg
     2221 gccccacgag ggccgcgagc tgtcccggtt cgatcgtgag ggagacgtct tccagcgtcc
     2281 attccctcgg cgcctcgttg gcgagctcga ggtcgctcgg ggagaccggt ccggcccgct
     2341 cgccggccgg ggtctcgtac cggaaccaca cgccgtccag ctcgacccga ccgagcaccc
     2401 ggtcgggggg gatcgcgacc gcatcgggcg aatcctcgat ctcgtggtcg aggtccaggt
     2461 actcgaagat gcggtcgaac aacgccatcg acgagtggac ctcggtcgag acctgcagca
     2521 tcgagccgat cgggaagaac aggcgcgact gcagcgtcgt gaacgccacg atcgtccccg
     2581 cggtgatcgt gctccccgtc gggtcccggc tgatcaccca cgcggccacg aggtacgcca
     2641 aggccggcgt gatcgagaag aacgtgccca cgatcgcgaa gaacgatcgc ccgatcatcg
     2701 tctggcgcat ctgcaggccg gtcaggcgct cgttctcgtc ccggaagcgg ccgatctcat
     2761 gacgctggcg tccgaaggac ttcgagagca ggatccccga gaccgacagg gtctcctcgg
     2821 tgaccgcggt gaggtccgcg tacgtcttgg ccgtgctcgt cgcgacctcc cgacgagcgc
     2881 gaccgacctt cacggtgagc cagaggaaga aggggagcat gaacagcgac aggacggtga
     2941 gctgccacga gaggagcagc atcgcgacca gcgtgctcgc gatcacgacg atgttcgaaa
     3001 ggatgttgga caccgtgtcg gtgaacaccg cctgcacccc accgacgtcg ttcgcgatcc
     3061 gcgactggat ctcgccggtg cgggtcgtgg tgaagaagcg cagcggcatg aactgcaggt
     3121 gcgaatagag cgcgttccgc aggtcctgca tgacgcgcag gcccacccag ttcgcgaggt
     3181 aggtctgccc cacgccgatc acgccggtga cgatggggat cgcgatcatc agcgcgacgc
     3241 cgatgtacac ggtgtcgagc tgtggacacg gctggccggc gcacaggttg ggcgggttgc
     3301 cgaacagccc gttgtcgaag atccacttga tcagcagggg gttcacgaca cccaggccgg
     3361 aggtcaccac gatggccagc ccgacccagg ccaccttccg gcggtagggc ttgaaggtcc
     3421 cgatcacacg ccggacggtc ttggctcgga tcggccggtc gtcctcttgc gccggggcgc
     3481 gcatgtacct gccgtgcatc accatggccc caaggataag acgcctctgc gacgtggtat
     3541 tccccgtggc ggcgttggcg tccctccccg cccgaggatc gatcagccgg cgagctcggc
     3601 cgcgcgttcg aacaccgcgt cgagcatctg cggcgtcagc ttgccggtga aggtgttctg
     3661 ctggctgggg tggtacgagc ccagcaggac gaacggctcc accttcgcct ccgccgcgtg
     3721 gccgaaccga ggcctcggcc tcgtcgggtg cccgagcgcg gacagcgcac ggagcgcccc
     3781 gtcccacgcg aacgccccga gcgccacgag cacgcggagc cgctcgaggg cggcgatctc
     3841 gcgttcgagg aaggggaggc acgtgtcgcg ttcggccggc gtcggcctgt tgtccggcgg
     3901 ggcgcatcgg ttgacggcgg acaggtacac gcggatcaac cggagcccgt cggcccgcga
     3961 caccgacgtg ggttggttcg acagaccgaa gcgatgcagg gaggcgaaca ggaagtcacc
     4021 cgagcggtcg cccgtgaaga tccggccggt ccggttcccg ccgtgcgcgg ccggcgcgag
     4081 cccgacgacc aggatcccgg cggccgggtc cccgaacccc ggaaccggcc gaccccagta
     4141 cgtctggtct gcgaatcgaa ccgtcttctc gcgcgcgacc cgctcgcgcc actcgaccag
     4201 acgggggcac gagcgacacc ccacgatctc cccggtgatc cgttccaggt cccgggcact
     4261 catccgcgca gctcgcggag gcgctcggac gcccgttcga acgcgtcgag cggcgcgagg
     4321 aggtccccga tcagaccgat cagcccctcg aacccctccg gctccgcacc ctgggtccgc
     4381 acccggtcgg caagcgcgag cgcctcgtcg agaccccgcg aacacgcctc ccactcgggc
     4441 tcgacgcccg tcgaacgcca ggcgtccatc ccttcgcgaa cctcgcggag cccgtcctcg
     4501 aactccgaca gcgtctccgc gagcgggcgc cccggcagac gcgttccggg caccgacccg
     4561 gtgagggcgg cctcggcgcg cccgagcggg ggcaggaggt cgtcgaacgc cctcgcgggc
     4621 ccctcgaggt gctccggcaa ccgtcggcga cgagggctca cgaacggacg tggtccgcga
     4681 gcacggcgag cgccatctcc gggtcgttgg acgcgaccgc gtcgaacccg cgatccaaca
     4741 gcatccgaac cgtctcgggg tcgtccaccg tccacgtccc cacacgaagg cccgccgcgt
     4801 gcacctgctc gacgaacgac ggtcccgccg ggatcgcggc ccgcgtgccg ggcaggacca
     4861 tgtcgtgccc ggcggaggcc gcgtaggcga gcgcctcccg cggcgggacg agatcggtcg
     4921 ttaggaaccc ggtcgggacg tccggggtga cgagcttcga ggcggcgatc gaggaggggt
     4981 tgaacgagat cacgaggacc ggcccgtcga acccttgccg ctcgagctcg gcgtggatcg
     5041 cctccacgat cggctcgcgg gtcggctcga acgccggctc gcccgggatg ttcttgacct
     5101 ccgccgcgat cgccgcccga ccgctgacgc agcgcaggac ctcggcgagc gtcggcacgg
     5161 gtgtgggctc ggcctcggtg ccggcgttca gcgaggagag gtcggccgcg ctgagctcgt
     5221 ggacgaggcc cgacccgccg gtcgtccggt cgaccgcggg gtcatggatc acgaccggca
     5281 cgccgtcgcg cgacagccgg acgtcgaact cgacgatgcc ggcgccgagc cggatcgcct
     5341 cctcgaacga tgggatcgtg ttctcagggc gggtcgatga ggcgccgcga tgcgcaacga
     5401 cgaacgggaa cgcgtcgttc gggaagcgat ggccccgcgc cattcagacg aggctcgagg
     5461 ggcagaggtg attcacgggg cagacctcgc acttgggctt gcgcgccacg cagagcgctc
     5521 tgccgtggtc gatcaggacg taggtgaagt cgaaccaccg ctcctgcggg atccgtcgca
     5581 tgaggtcctg ctcgatcttg tcgggatccg actcctggct gaacccgagc cggttcgcca
     5641 gccgcttgac gtgggtgtcg accgcgatgc cctcgaccgt gccgaacgcg ttgcccaaca
     5701 cgatgttggc cgtcttgcgc gctacgcctc gcagcgtgat caggtcctcc atcgtgtccg
     5761 gcacctgccc gccgtacacc tcgacgatcc gacggcaggc ctcccggatc gacgtggcct
     5821 tctggttgaa gaagccggtg ggcttgatgt cggccctcag ctcgtcctcg ggcaccttca
     5881 ggtaatcctc cgcccctcgg tacttccgga agagcgtcgc cgtcacctcg ttgaccttct
     5941 cgtcggtgca ctgggccgac aggatcgtgg cgacgagcat ctcgagcggg ttcgagaagt
     6001 tcagggcgac cttggcgtcg ggataggcct cggacaggat gcggatgatc gtgggggcgc
     6061 gttcctcgat cggcgcgtca cgcccgagcc ccttcggggc cttcccggcg ctcacgccgc
     6121 ctcgtcggcc aggggcagtg acagctcgat ctcgtcgccc tccgcgtacc tcgtccactc
     6181 cccctcgcgc atcacgtgga ccttggcccg accgatgacc tcccagctcc ggccgtctcc
     6241 gaccatcgcg gtgtcctcgt ccaacccgat gaaggtgccg ccgggttcta gcgatcccgc
     6301 gatgaactcc gtcgcgccgg ggatccacgt gtcgacgatg tcccagtgcg gtccgaacag
     6361 cgtgtcccgc acgtacccga tgcccggggc ccagatcgag tcgaagtcct ggctgtccga
     6421 gtcgtacgtc ttctcggtca ggcacgccac gcccgcgctg caccccgcgt aggggagacc
     6481 ggcgcggatc gccgcctgca gggcggccca gaacggggtc ccgaccatca cccgggcgag
     6541 ccgcgccggg ttgccgccgg agaagaagat cgccgatgcg ccttccagct tcgcgatcac
     6601 ctcgtcgcgg tgcgcgtcct cgcgggtctt gagcggaagg acctcggccg cgatcccgag
     6661 ggagccgtag tgctcgagac ccttgttggc ccagccgtcg tacgacgcct cgccctcgtg
     6721 cgccgacgcc gtcgcgcaca cgagcaccgg gccgtcgagg gtacgggacc gcgcgagcaa
     6781 ccaccgttcg acgggttcgg accacgggtc gaactcgccg gagccgagga acccgagcga
     6841 gatctcgctc acgttcgctc ctcccccggg ccgaacgcct cggtcgacat ctgtctccac
     6901 cacaggtccg gcccgaccgc ctccaagatc gcctcgacct ggctcacgtg cgcgttgatc
     6961 gcatcgacct tgagcgcgag gatgtcttcg ggcagctcgt agtagaccga gagctcgtcg
     7021 cgcgcggtga tcggtggggt tccgggtcgg aagacgttga acggctccca gaccgggagg
     7081 atctccgacg cgaactcggg cgtgaccgtc gcgtacagca ccctcgagcc gggctttccg
     7141 acctcgtgga gcgccttggt cgcccaggcc gacgcgtcct tgtgggcggc gtgcccagtc
     7201 atcccgtcgg gtccgaacgt gaggatcgtg tccggctgga cgtcggcgac gatctcgcgg
     7261 atccggtcgt agccctcatc gggcaggccg gtgtccatgt cgacgtcgcg catgtcgagc
     7321 cacacgtgct cgtcgacgcc gaggatctgc aggctccgct cgagctcctt ggtgcggacc
     7381 tcgcccatgg actcgggcgg ccacttctcc tcgtccatcg atccgccctc gcctcgggtc
     7441 gcggtcacgc aaacgacgcg cgagccgttc cgaacggccc gagccatgat ccccgccgtc
     7501 aggtacgcct cgtcgtcggg gtgcgcccac acgccgagga tgtttccgag ctcgtccatc
     7561 actcctcctc gcctcgcggc cgtcacgatg ggccggtccc ggacccaggt cccggcgctg
     7621 catcctagcg tcgggggcca gacgagggag atggacaata gaggtggatg caggagaacg
     7681 gcccgggaaa ccgggccgtt ctccgatccc acgcgaggga gggtgcgtgg gacagctcgt
     7741 acgatgacgc gtcaggcgcg gtcgttccgc agccgcttgc cgcggtagtt ggggaagacg
     7801 acatccgcat gctgccagca gaacgcgccc tcgttgtaga ccgatagcgt cgtccggcat
     7861 ccttcgtgcg cgcagacccg accgcgctcg agggtcctgg acggcgaggg caggttgccg
     7921 ggtcgcgatc ccttggtcac gttcccgatt cgacgctcgc agtcgagggg ttcggtcgcc
     7981 cgatcgggcc gtaagggtga gctggaccgg gcagtcatcg gcgagctagg aggggtgggc
     8041 gtgcgaccgc tcgagcagcg ctcggagccg gtcgacgagc tcgggtggca cctggtcaca
     8101 tccgcacttg gaccgcaaga cctccttgag tcgccgctgg aatgcggcgt gcccgctgca
     8161 ggggtcgcag ccacccaggt gttgctcgat ctcgacgcgc atggaagcga cgagctctcc
     8221 gtcgaggtag agctcgatct gcttcagcac gtgttcgcag tcagtcggtg acaaggcctc
     8281 gctcccgtac cgtttcccac aacgccttct gcagggcttt ccttccccgg tgcagacggg
     8341 acatcacggt cccgatcggg acgtccatga tctccgcgat ctccttgtag ctgaacccct
     8401 cgacgtccgc cagcagcacg gccatccgga acccctcggg caggtcctcg agcgccctct
     8461 ggacgtcctc gtcggggagt ttctcgagca cctcggactc ggccgacgcc tcgacgttcg
     8521 aggcaccgag cttgtcgtac aggtaccagt ccggggtgtc gtcgtcgggc acggtgaggg
     8581 gctcgcgctg cttcttgcgg taggaattga tgaacgtgtt ggtcaggatc cggtagaccc
     8641 aggcccgcag gttcgtgccc tctcggaacc cggagaagcc gcggtaggcg cgcaggtagg
     8701 tctcctgaac caggtcctcg gcgtcctgag ggttccgggt catccgcagc gcggccccgt
     8761 acaggttcgg cagcagcggg aggacctcgc gctcgaatcg ctcgcgaagg gcgggatcct
     8821 ccgggggcgc caccgggctc cgcgccaccg ccgatcctcc ttcccgctct ctggcccgct
     8881 tgcttggccg ggtctcgttc ccggctcgct cgccgagcga accctcagta tcccgcatcg
     8941 accgggacct ccaaccaccc gcatgtcgtt cggcgaacat cgatgggaag atgggaggcc
     9001 agtggcgaac aacgacggca aacgcggacg ctggcagggg ttcgatctgg ggggtggagc
     9061 gcccagcgga ccccgtcggg tgcgctcgtc gccgttggtc tgggcggtgg tcttcgtcct
     9121 gatcctgatc gcattccagt tcctcacgtc cgcccgcccg cacacgatcg actactcgaa
     9181 cttcctcgag cgggtcaagg acggccagat cgtcgggacg ctcgagatca gccagacctc
     9241 ggtctctggg cggtacgagg actcgacggg gaccgttgac ttcaacacca cgattccccc
     9301 gatcctgcag gggacctcga ccctgacgaa ccagctcgac gccgccggga tcagctacac
     9361 gggcgagacc cccggcggcc tgcagaccat cctgctggcg tgggtcgtcc cgatcctgct
     9421 gttcgggttc gtctggttct tcctcgtgcg acgcatgagc ggcgcacagg cgggcgcggc
     9481 gctgaacctc ggccggaaca aggtcaagat ctacgaccgc aaggagatga agacgacctt
     9541 cgccgacgtc gcgggggtgg acgaggcgaa ggaggagctc aaggagatcg tcgagttcct
     9601 gcgcaacccc aagaagtacc agcggctcgg tgggcggatc ccgaaggggg tgctgctcct
     9661 cggtcccccg ggatgcggca agacgctgct cgcccgcgcg gtcgccggcg aggccaacgt
     9721 gccgttcttc ttcatgtcgg gctccgagtt cgtcgagatg ttcgtgggcc tgggcgccgc
     9781 tcgggtccgc gagctgttcc agcaggccaa ggagaaggcc cccgcgctcg tgttcctcga
     9841 cgagatcgac acgatcggca agggcagggg cggggcgctg ggggcggggt tcggcgcgca
     9901 cgacgagcgc gagcagacgc tcaaccagct gctggtcgag atggacgggt tcgactcgca
     9961 gaagggcgtg atcatcatgg cggccaccaa ccgccccgac gtgctcgacc ccgcgctcgt
    10021 gcgcccgggc cgcttcgacc ggcaggtcgt cgtcgaccag cccgatctgc gtggccgcga
    10081 ggagatcctt caggtccacg tccgcggcgt cgcgctcgac cggcaggtcg acctccgcgt
    10141 cctcgcctcc cggacgccgg ggttcaccgg cgccgacctc gccaacgtcg tgaacgaggc
    10201 ggccctgctc gccgcccgca gggagcggga ggcggtcacc atggtcgagc tcgaggaggc
    10261 gatcgatcgg acggtcgcgg gtctggaacg caagagtcgc gtcatgtccg tgaaggacaa
    10321 ggagcgggtg gcgatccacg agatggggca tgcgctcgtc gccctcaccg cggagaccgc
    10381 cgatccggtc caccgggtct cgatcatccc gcgcggcgcg gcggcgctgg ggatgacgat
    10441 gcagcgaccg ctcgaggatc ggtacctgct caccgagccc gagctcaagg accgcctcgc
    10501 ggtgctcctc ggcgggcgca cggcggagga gatcctgttc gacctggtgg tctcgaccgg
    10561 tgcgcagaac gacctcgagc gcgccaccga gatctcccga gccatggtta ccgagtacgg
    10621 catgagcccg aagatcggcc cggtgtcgtt cgggaacgac gggttccgcc agggagccgg
    10681 gttcctgtac ccgggcggct cccccgagct ctcggacgac ctggcggcgc tgatcgacga
    10741 ggagaccgcc cggctcgtga acgaggccca cgagcgcgcc accgaggtgc tcacacgtta
    10801 ccgagggctc ctggagaagc tctcgaagat cctgatcgtg atcgaggtga tcgacgggcc
    10861 ggacctgcag gcgtacttcg acgggagcaa gccgatcccc acgccggagg agctcgagga
    10921 ccagatccac cggaacgccg gcacggcgac ccccggcacg gccgcccccg gctcgggcgg
    10981 gccggacatc gtcctgcagc cgcgcccctc ctagccgtcg gtcggccgcc gcacgagcag
    11041 gtcgtagcgg tcccggaacc gctcgaggaa ccggtagtag ttgcgcttgg agagccggtc
    11101 gcgttcggcc tcgggggtcg tgtggtacat ccggtgctcg tgcttgacga ccggcgcgtc
    11161 gacacgaagg gcccgcagac cggcgtcctt gatgcggaac gagtactcga tgtcggccgt
    11221 ccggtaccag cggaacttct cgtcgaacaa ccccacgtca cggatcacgt ccctgcggaa
    11281 cgccatcatg tacccctcga tcgcgtcgac ctcgggcccg tcgctctcgt ggaactcacg
    11341 caggtcctcg gtcgagatcc cgaacggccc gcagacgccg acgctcgggt cttcgagcgc
    11401 tgcctcgagc ggcgcgaaca cgtcccccgt gggttcgacc gatccgtcca tcacgagcac
    11461 ggaccggccg cgcgaacgcc tgagccccgc gttcagggcg gcgccccagc ccgtcccggt
    11521 ctcgagcgcg atcacctcga cgcccgcccc gtaggtcgag gggtccgtct cggtgaggtc
    11581 cgcgacgacg taccgcacgt cgcggcctcc ctcgttcgcg cggaagccgg cgataccacg
    11641 cgcgacgtcc tcgggccatc cctcgacgac ccaatgcacg ctcgcgtcgg cggtcgaggg
    11701 ctcgtcgagc aggctctcga cgtcgtgcgc ggcgatgcgg tggagttcgg cgacggcgag
    11761 tggctccagc gagggtccat ccgggccgtc gaccaccgag aagcccagcc gtgcgatgcg
    11821 atcgcggagc gcgtccgcgg cggcgaagtc cttggcccgg cggcgctcgt cgcgttcggc
    11881 cgcgagcgcg cggacctcgt cggggacctc ggtcatggcc tttgcccgtg ccggtggact
    11941 agacgggctc cttcgagacc gagcgcagtc gcccggccac ggcctccagc gtcttgaggg
    12001 cgatcgtcgg atgctccatc agcaggcccc ggaacaccca gccggtgatg ccgacgaggc
    12061 ggaccagtga gaccgccgtc acggtcgcgg tccgggggcc gccgtccagc agggctacct
    12121 ccccgaagaa gtccccggct cccagacgcg ccttggtctt gccgccgatc gtgaccttgc
    12181 actggccgtc caggatcagg aacaggccga tcccgcgctc gccttcgcgg gcgatcacgg
    12241 ttccttcctt gtgcgagacc tccttggacg cccgggcgat gatcccgagc tcacgctttg
    12301 agcagccgga gaagatcggc acgctggcga ggttgttcgc gacgctcggg gctgccacct
    12361 gccgctccac ctccgttccc ggttcggttc ctgctccgtc cccggctccg ctggtggctc
    12421 gttcggtcct tcgagcccgc cagtcggggc acgcgatcct atcgcgatcc gatcgacggg
    12481 cgcgaaccgc tacgatgctg ccgtgagcga gcaacgacgc ttcgcggcga tggtcgtcct
    12541 gttcctgatc gggttcggac tcatcgggtt ggcgagcgcc gtggattcga gctggccgct
    12601 cttcgtcacg gtgatcccct cctcgatcat cccgtggctc gtggtccacg
//
LOCUS       DATBRK010000055         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2607109, whole genome shotgun sequence.
ACCESSION   DATBRK010000055 DATBRK010000000
VERSION     DATBRK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2607109"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..453
                     /locus_tag="VIH70_01700"
     CDS             121..453
                     /locus_tag="VIH70_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8115109.1"
                     /translation="MLHGVLFLDEFAEFRRDAIEGPRQPLAIRSQVRAASNGICRRRD
                     TVRRAQQRHTRQSVGPAVWVCARRLRRDGSGRHDGRLPTEALPDVEEGRVAEAARRRS
                     WPRPRANG"
     gene            complement(807..>2082)
                     /locus_tag="VIH70_01705"
     CDS             complement(807..>2082)
                     /locus_tag="VIH70_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8115110.1"
                     /translation="KHLPSAGGFYTYTARGIHPSIGFLVAWGYAFVEPLVAPALFLIF
                     GQVVASTLDIEFGWSFSTWWWIAALAAAIVVFLLGFYGIQISTRVGTLLGLFEIGVFT
                     ALAIWLIVDAGSDNTLKVFGTSFATVPGFKGLSGVIAGSVYTILAFIGFEAAAPLAEE
                     ARDPRRTIGRAVVLSCIGIGIFYVLTTYAAVVFFGPGRFAEFPMSGGGNPWDALARAA
                     WGAGWVLVFLAIANSAIANSNAGSNAATRTWFAMGRIHLLPAMLGRTHPRYRSPHVAV
                     LVQFVIGVGVALWLGAQYNSPFLAFAILGTIIGAVVIGIYMTVNLACLLYYAREQRNE
                     FNWLLHGVIPILGILAFVPAFLVAIGVGGSVFDFVSKLPYPFSVVGPVVGIWYVLGIV
                     YLIYLSARKPERVKDTARVFIEEEVAEVSAPI"
BASE COUNT          383 a          721 c          654 g          324 t
ORIGIN      
        1 gtctcgtacg gtgctcgcca tcctcacccg tcctgtgtga gtctctcggt aggtatcgcg
       61 cctcaacgct cagagcttga ggtgaccacc tgtgaactca ggcgacattt gtgaacactg
      121 gtgctccacg gggtgctgtt cctcgacgag ttcgcggagt tccgccgcga cgcgatcgag
      181 ggcccgcgtc agccgcttgc gatcagatca caggttcggg ctgcttccaa cggcatctgc
      241 cgtcgtcgtg acactgttcg gcgcgctcag caacgacata cacggcagtc ggtcggcccg
      301 gctgtttggg tttgcgctcg ccgccttcgt cgcgatgggt ctggtcggca cgatggccgt
      361 cttcccaccg aggctctacc ggacgtcgag gaaggacgcg ttgcagaagc tgcccgacgg
      421 aggtcgtggc ctcgaccaag agccaacggt tgaatggcta tcaagcatga tcgacctgga
      481 gaggaagctc acgagtcgtt tggaaacggc gttgggagag gaacgcggcg cccttctgct
      541 ggttcagggg ctgttcgtgg cgcagatcgt cctcctagga tggataaccc tgagttccgc
      601 ctggatgttg gccgggaatc ggcgagcgcc catcgcgccg agcctctcga acgggaatct
      661 gcctagaccg cgcgctcgcc gtgcggcgcg ggtgtcgtat tcgggctctc gtcgcccaag
      721 tcgatggatg ccccttcatg tggcgccggc gaccgtgcct cgctcaccgg cggctgcgcc
      781 tctgcacccg tggcggccga ggccgctcag atcggcgccg acacctccgc gacctcctcc
      841 tcgatgaaca cgcgcgctgt gtccttcacg cgctcgggct tccgcgcgga taggtagatg
      901 aggtacacga tcccgagcac gtaccagatc cccaccaccg gcccgacaac gctgaacggg
      961 tacggaagct tgctgacgaa gtcgaacacc gatccgccga cgccgatcgc gaccaggaac
     1021 gccgggacga acgccaggat ccctaggatc gggatcacgc cgtgcagcaa ccagttgaac
     1081 tcgttccgct gttcgcgcgc gtagtagagc aggcacgcga ggttcaccgt catgtagatc
     1141 ccgatcacga ccgcaccgat gatcgtgccg aggatcgcga atgcaaggaa tgggctgttg
     1201 tactgggcac cgagccacag tgcgacgccc accccgatca cgaactgcac caacaccgcc
     1261 acgtgcggcg agcggtacct cgggtgcgtt cgcccgagca tcgccggcag caggtggatg
     1321 cgacccatcg cgaaccacgt ccgcgtcgcg gcgttcgagc cggcgttcga gttcgcgatg
     1381 gccgagttcg cgatcgcgag gaacaccagc acccagcccg cgccccaggc cgcccgcgcc
     1441 agagcgtccc acgggtttcc gccgcccgac atagggaact cggcgaatcg cccgggtccg
     1501 aagaacacga ccgccgcgta cgtcgtcagg acgtagaaga tgccgatccc gatgcacgac
     1561 aggaccacgg cacggccgat cgtgcgccgc ggatctcggg cctcctccgc cagcggcgcc
     1621 gcggcctcga acccgatgaa cgcgaggatc gtgtacaccg acccggcgat cacgcccgac
     1681 aaccccttga accccggtac cgtcgcgaac gacgtgccga acaccttcaa cgtgttgtcg
     1741 ctgccggcgt cgacgatcag ccagatcgcc aacgccgtga acacgccgat ctcgaacagc
     1801 ccgagcagcg tcccgacgcg cgtgctgatc tggatgccgt agaaccccag caggaacacg
     1861 acgatcgccg ccgcgagcgc tgcgatccac caccacgtcg agaacgacca gccgaactcg
     1921 atgtccaacg tcgaggccac cacctgcccg aagatcagga acagcgcggg cgcgaccagc
     1981 ggctcgacga acgcgtaccc ccacgccacc aggaagccga tcgagggatg gatccccctg
     2041 gccgtgtacg tgtagaagcc gccggccgaa ggcaggtgct tc
//
LOCUS       DATBRK010000056         7400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_293815, whole genome shotgun sequence.
ACCESSION   DATBRK010000056 DATBRK010000000
VERSION     DATBRK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293815"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VIH70_01710"
     CDS             <1..1012
                     /locus_tag="VIH70_01710"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115111.1"
                     /translation="AKGVFELVSRLVQEGQDLRHVTNEVLAHFRNLLLVKTAPGQHDL
                     LDATDEEAERLTAQASKYSAAELGRVIALLIAAQTDMRWTTSPRLSLELALIRSTIPE
                     TDAAPEALVSRLERLERVAGITGGGRSAGGGSQSPDTATPEPPTPSDTSATTAGPGGS
                     GTGEAADPAPPAVETKARRPTKRTAASSAEEAASGPVPSGTEAGAVPVLGAGASAVDV
                     SMLRSNWPRVISHLRSSGKAVLPSFLEIATPGAFDGTTLELVFPPERPYGVQKVLERE
                     GELRQALQDLFGIAPAISCVIREPVAGPAEPIEDEPLSEEEALARLAAELGATPATED
                     GS"
     gene            1013..1606
                     /gene="recR"
                     /locus_tag="VIH70_01715"
     CDS             1013..1606
                     /gene="recR"
                     /locus_tag="VIH70_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY8115112.1"
                     /translation="MYEGPIQDLIDELSRLPGIGPKSAQRLAFHLVKAPPDDAKRLAE
                     SIVQAKERIFFCRECGNVAEGELCRICRDEGRDRTTLCVVEEPKDVSTIEKAGVIKGR
                     YHVLGGAISPLDGVGPEDLRVQELLDRVERDGVTEVILATNPNLEGNATAMYVAAMLK
                     PFGVRVTRLASGLPVGGDLEYADEVTLSQALEGRREM"
     gene            1608..2252
                     /locus_tag="VIH70_01720"
     CDS             1608..2252
                     /locus_tag="VIH70_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115113.1"
                     /translation="MSEAAKETVRRDRRVELIAAIMLSAATVVTAWSAYQATRWSGDQ
                     SENYTQASATRTESVRSSTEANRQVLIDVDTFLNWLDARQSGDPDLADDIHARMREEF
                     LPAFNAWLASAPAGSIPDGTPFELPEYRLAAEEEAKRLETKASALFEDGNESNQVSDD
                     FVLAAVLFASVLFFSGLAGTFDSLRAQVFLLILGAVMLAIGAIIVITLPQNVGF"
     gene            complement(2249..3199)
                     /locus_tag="VIH70_01725"
     CDS             complement(2249..3199)
                     /locus_tag="VIH70_01725"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY8115114.1"
                     /translation="MATTIEYLNSVHGDLLDAAEREQRAARRPPSPRRMPSSGLVAAV
                     VSVLVAAGLIGLIVRSGGLVSEGGGESLGGSTGAMGATGAPTEPPSDEVVPGAPAVSR
                     VIRTAELAVVIPRDAFDERFGEAVDVAEAQGGFVADSRSRSRAGSLTLRVPAANFDET
                     LRELRALGTVEVESVHGEDVTADYVDLHANLRIARARREVLLGLMADAVSIEQTIRVQ
                     NALDETQLRIEQLQGQLRLLDDRTSLATIGLRLREQGVEPESRVEKASIPNAFERAAA
                     GFVGVIAAIVIGLGYLIPILVLGLAAWFVVSRVRRRRAAG"
     gene            complement(3175..3789)
                     /locus_tag="VIH70_01730"
     CDS             complement(3175..3789)
                     /locus_tag="VIH70_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8115115.1"
                     /translation="MHEGLDDAELILRSSREPELFGLLFERHAEAMLAFFARRTLDAE
                     SAAELVAETFAEAFSSRRRFRDEGVDGVGWLYGIGKHLLSRYFRAGAVEARARRRLGM
                     PERTVSDDDYDRIEELIDFERFGGALDEAMSSLPEEQQEAVRLRVMDQRSYGEVAAEL
                     GCTEPTARMRVSRGLRRIAGVLETDTSEAPIELERRRWQRRSST"
     gene            3867..5957
                     /locus_tag="VIH70_01735"
     CDS             3867..5957
                     /locus_tag="VIH70_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8115116.1"
                     /translation="MPETQDPLDEFPRQVARTRGFSLGVPRSFTVSPDGERLVYLRTK
                     TGDDPVSCLWVFEVSGAKERLVFDPGEEEAERDLTEAERARRERSRERSSGVTAYATD
                     EAVRRAVFTVGGRMFLTDLVEGSARELSAPGSVDDPRLDRSGERIAFVVDGTLHVREI
                     EGGVRELASPDAPDTFWGLPEFVAAEEMRRFRGHWWSPDGSKLAAAHVDEREVQVWHI
                     GDPTDPAAEPRAVRYPQAGTNDAVVTLWVFDVARGERVEVRWDRERFPYLTRVTWEHG
                     PLSILVQTRDQRAVSLLEVDDSSGETTVVSEQSDPRWVELAEDAPVRLSDGRVVDVVA
                     DPETDTYVLTVAGELVTPAGLQVREVLSADAGVLFRASDGDPTEIHLWRWTPREGALR
                     LSEEPGVYSGAEGGEVLVRVSATPHSVRSVATVWRSGEQIGTIENVSEVPVIEPRPRF
                     LTLGERELHAALLLPGGREPEGPLPVLMDPYGYPRGARVLKSAAAHLTSQWFADQGFA
                     VLVVDGRGVDGRGPSWDRMVYRDFSLVLEDQVDALQSAADRFGFLDLGRVGIRGWSGG
                     GVLSALAVLRRPDVFHAGIVGAPVTDMRLYDTHYMERYLGRPQDEPEAYRRSSPLPDA
                     PALSRPMLLIHGLADDNVFVANTLQLSAALFEAGKWHELVLIPNATHLTRSTAVTENV
                     LRIQLDFLKRTLVR"
     gene            complement(5959..6288)
                     /gene="fdxA"
                     /locus_tag="VIH70_01740"
     CDS             complement(5959..6288)
                     /gene="fdxA"
                     /locus_tag="VIH70_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012731278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY8115117.1"
                     /translation="MAYVIAEPCIDIKDKACVDECPVDCIYEGPRMLYIQPDECVDCD
                     ACVPVCPVTAIFSDEELPGEWKQFEAVNAEWFSDAVTGLGSPGGAAQAGPQDKDHKVV
                     AEWPAVA"
     gene            complement(6378..6815)
                     /locus_tag="VIH70_01745"
     CDS             complement(6378..6815)
                     /locus_tag="VIH70_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8115118.1"
                     /translation="MEGEAPGARRLIDAERRVHRILRAQFTDALRDLGTSFAQVEVME
                     LLHQAVQLHPGEIGRRLLITRQSATHLVRQLERASLVETWRLGGGSVGVRLTETGRES
                     MVHCFDALLPTFDLIEALDEDRRSRLVKDLRAWENALHPRLLP"
     gene            7113..>7400
                     /locus_tag="VIH70_01750"
     CDS             7113..>7400
                     /locus_tag="VIH70_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY8115119.1"
                     /translation="MPIVVQKYGGTSVGDTDRIRGVADRIVRAREQGQDVVVVVSAMG
                     ETTDDLLGKASDITADPEPRELDMLLTSGERIAMSLLAIAVNARGCRAASYT"
BASE COUNT         1081 a         2632 c         2593 g         1094 t
ORIGIN      
        1 cgcgaagggc gtcttcgagc tcgtgagccg tctcgtgcag gaggggcagg acctgcggca
       61 cgtcaccaac gaggtgctcg cgcacttccg caacctgctg ttggtcaaga ccgcgccggg
      121 ccagcacgac ctgctcgacg ccaccgacga ggaggccgag cgcctgaccg cgcaggcctc
      181 gaagtactcg gcggccgagc tcggtcgcgt gatcgcgctc ctgatcgccg cgcagaccga
      241 catgcgctgg accacctccc cccgcctctc cctcgagctc gccctgatcc gctccacgat
      301 ccccgagacc gacgccgccc cggaggcgct cgtctcccgc ctcgaacgcc tcgaacgagt
      361 ggccggcatc actggtggtg gccgctccgc gggcggcggc tcccagtcgc ccgataccgc
      421 aacccctgag ccgccaacac cgtccgacac gagcgcaacc acggcggggc cgggcgggtc
      481 cggaaccggg gaagcggccg acccggcgcc ccccgcggtc gaaacaaagg ccaggcgacc
      541 caccaagcga accgcagcga gcagcgccga agaggccgcg tccggtccgg tcccgtccgg
      601 tacggaggcc ggggcggtgc cggtcttggg tgccggggcg tcggcggtgg acgtgtccat
      661 gctgcggagc aactggccga gggtgatctc gcacctgcgg agctcgggca aggcggtgct
      721 cccctcgttc ctcgagatcg cgacgccggg ggcgttcgac gggacgacgc tcgagctggt
      781 ctttcccccc gagcgcccgt acggcgtcca gaaggtgctc gagcgcgagg gcgagctccg
      841 gcaggcgctc caggacctgt tcggcatcgc gcccgcgatc agctgcgtga tccgcgagcc
      901 cgtggccggt ccggccgagc cgatcgagga cgagccgctg tcggaggagg aggcgctcgc
      961 gcgactcgcg gccgagcttg gcgccactcc cgcgaccgag gacggctcct aggtgtacga
     1021 gggcccgatc caggacctga tcgacgagct ctcgcgcctc ccgggcatcg ggcccaagtc
     1081 ggcgcagcgg ctcgcgttcc acctggtgaa ggcgccgccc gacgacgcca agcggctcgc
     1141 cgagtcgatc gtccaggcga aggaacgcat cttcttctgc agggagtgtg gcaacgtcgc
     1201 cgagggcgag ctctgccgca tctgccgcga cgaggggcgc gaccgcacga cgctctgcgt
     1261 cgtcgaggag ccgaaggacg tctcgacgat cgagaaggcg ggcgtgatca aggggcggta
     1321 ccacgtgctc ggcggcgcga tcagccctct cgacggcgtc ggccccgagg acctccgagt
     1381 gcaggagctg ctcgaccgcg tcgagcgcga cggcgtgacc gaggtgatcc tcgcgaccaa
     1441 cccgaacctg gaaggcaacg ccaccgcgat gtacgtcgcg gcgatgctga agccgttcgg
     1501 ggtgcgggtg acgcggctcg caagcggcct gccggtcggc ggggatctcg agtatgccga
     1561 cgaggtgacc ctgtcgcagg ccctcgaggg ccgtcgcgag atgtgacgtg agcgaggcag
     1621 ccaaggagac ggtccggcgc gaccgacgcg tcgagctgat cgccgcgatc atgctctccg
     1681 ccgcgacggt cgtcacggcg tggagcgcgt accaggccac ccgatggagc ggcgaccaat
     1741 cggagaacta cacgcaggcg tccgccaccc gcaccgaatc ggtgcgctcg tcgaccgagg
     1801 cgaaccgcca ggtcctgatc gacgtcgaca cgttcctgaa ctggctcgac gctcgacagt
     1861 cgggcgatcc cgacctcgcc gacgacatcc acgctcggat gcgcgaggag ttcctcccag
     1921 cgttcaacgc gtggctggct tcggcgccgg cgggttcgat ccccgacggc acgccgttcg
     1981 agctcccgga gtaccgcctc gccgccgagg aggaggcgaa gcggttggag accaaggcct
     2041 ctgcgctgtt cgaggacggg aacgagtcga accaggtgag cgacgacttc gtcctggccg
     2101 cggtgctgtt cgcctcggtc ctgttcttct cgggcctggc ggggacgttc gactcgctcc
     2161 gcgctcaggt cttcctgctg atcctcggcg cggtcatgct cgcgatcggc gcgatcatcg
     2221 tgatcacgct cccccagaac gtggggttct agccggcggc gcgacgcctt cgcacgcgtg
     2281 agaccacgaa ccacgcggcg agcccgagca ccaggatggg gatcaggtac ccgagcccga
     2341 tcacgatggc ggcgatcacg ccgacgaacc cggcggcggc ccgctcgaac gcgttcggga
     2401 tgctcgcctt ctcgacccgg gactccggtt cgacgccctg ctcgcgcagg cgaaggccga
     2461 tcgtcgccag cgaggtgcga tcatcgagca gccggagctg cccctggagc tgctcgatgc
     2521 gcagctgcgt ttcgtcgagc gcgttctgca cgcggatcgt ctgctcgatc gagaccgcgt
     2581 ccgccatcag cccgagcagg acctcgcgac gagccctcgc gatccgcagg ttcgcgtgga
     2641 ggtcgacgta gtctgccgtg acgtcctcgc catggacgga ctcgacctcg acggtcccga
     2701 gcgcgcgcag ctcgcggagc gtttcgtcga agttcgccgc ggggacgcgc aaggtcaggc
     2761 ttcccgcccg gctccggctt cgggagtcgg cgacgaaccc accctgcgcc tccgccacgt
     2821 cgacggcctc cccgaaccgc tcgtcgaacg cgtcgcgggg gatcacgacc gcgagctcgg
     2881 cggtgcggat cacccgcgag acggccggcg cgccggggac gacctcgtcg ctcggaggct
     2941 ccgtcggcgc gcccgtggca cccatcgctc cggtcgatcc cccaagcgat tccccgccgc
     3001 cctcagatac gagcccgccc gaccgcacga tcaggccgat gagacccgcc gcaaccagga
     3061 cgctgacgac ggccgccacc agcccgctcg agggcatccg gcggggggag ggcggacggc
     3121 gggcggcgcg ctgctcccgt tcggccgcgt ccagcaggtc cccgtggacc gagttcaggt
     3181 actcgatcgt cgttgccatc gtctccgctc cagttcgatc ggtgcttcgg acgtgtcggt
     3241 ctcgaggacg ccggcgatcc gccggaggcc ccggctcacg cgcatccggg ccgtcggctc
     3301 ggtgcatccg agctccgccg cgacctcgcc gtagctcctc tggtccatga cccgcagcct
     3361 caccgcttct tgttgctctt ccggcaggga gctcatcgcc tcgtcgagcg ccccgccgaa
     3421 ccgctcgaaa tcgatgagct cttcgatccg gtcgtagtcg tcgtcgctca cggtgcgctc
     3481 tggcatcccc agtcgccggc gggccctcgc ctccaccgca ccggcccgga agtaccgaga
     3541 gagcaggtgc ttgccgatcc cgtagagcca cccgacgccg tcgaccccct cgtcccggaa
     3601 ccggcgccgg gacgagaacg cctcggcgaa cgtctcggcc acgagctccg ccgcggactc
     3661 ggcatccagc gttcgccggg cgaagaacgc gagcatcgct tcggcgtgcc gctcgaacag
     3721 caggccgaac agctccggct cgcgtgagga ccggaggatc agctccgcgt cgtcgagtcc
     3781 ctcgtgcatc ccaccctcgt tcgttcctac gagtcccatg ccgttcgggg cccgtcgtgt
     3841 gacacgccgt tggtaggctc ggccccatgc ccgagacgca ggacccgctc gacgagttcc
     3901 cgcgccaggt cgcccgaacg cgcggcttct cgctgggcgt gcctcggtcc ttcaccgtct
     3961 cgcccgacgg ggagcggctc gtctacctgc gcacgaagac gggcgacgac cccgtcagct
     4021 gcctttgggt gttcgaggtc tcgggggcga aggagcggct ggtgttcgac ccgggggagg
     4081 aggaggccga gcgcgacctc accgaggccg aacgcgcacg gcgcgagcgc tctcgcgagc
     4141 gctcgtcggg ggtgacggcg tacgcgaccg acgaggcggt ccggcgggcg gtgttcaccg
     4201 tcggcggcag gatgttcctc accgacctgg tggaggggtc cgcgcgcgag ctgagcgcgc
     4261 cgggttcggt cgacgacccg cgtcttgacc ggtcgggcga gcggatcgcg ttcgtggtcg
     4321 acgggacgct ccacgtccgg gagatcgagg gaggcgtgcg cgagctggcc tcgcccgacg
     4381 cccccgacac gttctggggc ctgcccgagt tcgtcgcggc cgaggagatg cgccggttcc
     4441 ggggacactg gtggtcgccg gacggctcga agctcgcggc cgctcacgtc gacgaacggg
     4501 aggtgcaggt ctggcacatc ggtgacccaa ccgacccggc ggccgagccg cgcgcggttc
     4561 gttatccgca ggccgggacg aacgacgccg tcgtgacgct gtgggtgttc gacgtcgcga
     4621 ggggcgagcg agtcgaggtt cggtgggatc gcgagaggtt cccatacctc acgcgggtga
     4681 cgtgggaaca cgggccgttg tcgatcctgg tgcagacgcg ggaccagcga gccgtgagcc
     4741 tcctcgaggt cgacgactcg agcggcgaga ccacggtcgt gtcggagcag tcggacccca
     4801 gatgggttga gctcgccgag gacgcgccgg tccgcctctc cgacggccgt gtcgtggacg
     4861 tcgtcgcgga tccagagacc gacacctacg tcttgacggt cgcgggcgaa ctcgtgacgc
     4921 cagcgggcct gcaggtccgc gaggtcctgt cggccgacgc gggcgtgctg ttccgagcct
     4981 ccgacggcga cccgaccgag atccacctgt ggcggtggac gcctcgcgag ggggctctgc
     5041 ggctgtccga ggaacccggg gtctactcgg gcgccgaagg cggggaggtg ctcgtgcgcg
     5101 tctcggcgac cccgcactcc gtgcggtcgg tcgcgacggt gtggcgatcg ggcgagcaga
     5161 tcgggacgat cgagaacgtc tcggaggtcc cggtgatcga gccgcgaccg cgcttcctca
     5221 cgctcggcga acgtgagctg catgccgcgc tcctgctgcc gggcggccgc gaacccgagg
     5281 ggccgctgcc ggtcctgatg gatccgtacg gctacccacg gggtgcgcgc gtgttgaagt
     5341 cggcggcggc gcacctcacg tcgcagtggt tcgccgacca ggggtttgcc gtcctggtcg
     5401 tggacggtcg cggcgtcgac ggccgcgggc cctcgtggga tcggatggtc taccgcgact
     5461 tcagcctggt cctcgaggac caggtcgacg cgctccaatc ggccgcggat cggttcgggt
     5521 tcctcgatct gggacgggtc gggatccgcg gctggtcggg cggcggggtg ctctcggcgt
     5581 tggcggtgct gcggcggccg gacgtcttcc acgccggcat cgtcggcgcc cccgtcaccg
     5641 acatgcggct ctacgacacc cattacatgg agcggtacct cggacggcct caagatgagc
     5701 ccgaggcgta ccggcggagc tcgccgctgc cggatgcccc cgctctgtcc cggccgatgc
     5761 tgctgatcca cggtcttgcc gacgacaatg tcttcgtcgc gaacaccttg cagctgtcgg
     5821 cggcgctgtt cgaggcgggc aagtggcacg aactggtgct gatcccgaac gccacgcacc
     5881 tcacgcgctc gacggcggtg accgagaacg tcctgcggat ccagctcgac ttcctgaagc
     5941 gaacgctcgt tcgctagctc aggcgacggc cggccactcg gcgacgacct tgtggtcctt
     6001 gtcctgcgga cccgcctggg ccgctccgcc gggtgagccc agccccgtca cggcgtccga
     6061 gaaccactcg gcgttgacgg cctcgaactg cttccactca ccggggagct cctcgtccga
     6121 gaagatcgcg gtgacggggc acaccggcac gcaggcgtcg caatccacgc actcgtccgg
     6181 ctggatgtag agcatccgag ggccctcgta gatgcagtcg accgggcact cgtcgacgca
     6241 cgccttgtcc ttgatgtcga tgcacggttc ggcgatgacg taggccatga gtcgtgggtt
     6301 ccccggtgtc gaggacccga aacctatctc gtggcgatgg gcgagctggg tgccccctag
     6361 aaggggtcct tgacccacta gggcaggaga cggggatgaa gcgcgttctc ccaagctcgg
     6421 aggtccttca cgagccggct ccggcgatcc tcgtcgagcg cctcgatcag gtcgaacgtc
     6481 gggaggaggg cgtcgaagca gtggaccata ctctcccggc cggtctcggt caggcggacg
     6541 ccgacgctcc cgcctcccag ccgccaggtc tcgaccagcg aggcccgctc gagctggcgg
     6601 acgaggtggg tggccgattg ccgagtgatg aggaggcgac ggccgatctc gccggggtgc
     6661 agctggactg cctggtgcag cagctccatg acctcgacct gcgcgaacga cgtcccgaga
     6721 tcccggagcg catcggtgaa ctgggctcgc aggatgcgat ggacgcgccg ctccgcgtcg
     6781 atcagtcgcc gagcaccggg agcctctccc tccaccggaa ggtcttaccg caaggcgccg
     6841 acatgtcgag agggttcacg agaggggtgc tgtccccctc gctcggagcc acccgtcaga
     6901 gttctgacag acagaaccgt gacaccgacc ccgaccgcct cgattccgcc ccagtctgtc
     6961 agggttctga cagacagaag cgtgacaccg accgtctcgg ttccggcccg gccgtcaggg
     7021 ttctgactga cagaaccctg acgggtccgc tcgagcgacc gcccctcctc tcttaggcga
     7081 acccgccgaa tcctcgctgc taccctcccc gcatgcccat cgtggtgcag aaatacggcg
     7141 gaacctcggt cggcgacacc gaccgcatcc ggggcgtcgc ggaccggatc gtccgcgcgc
     7201 gcgagcaggg ccaagacgtc gtcgtcgtcg tctcggccat gggcgagacg acggacgacc
     7261 tcctcggcaa ggcctcggac atcacggccg accccgagcc gcgcgagctc gacatgctct
     7321 tgacctcggg cgagcgaatc gcgatgtccc tcctcgccat cgccgtcaac gctcgtggct
     7381 gccgggcggc ctcgtacacc
//
LOCUS       DATBRK010000057         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_727719, whole genome shotgun sequence.
ACCESSION   DATBRK010000057 DATBRK010000000
VERSION     DATBRK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727719"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1500)
                     /locus_tag="VIH70_01755"
     rRNA            complement(<1..>1500)
                     /locus_tag="VIH70_01755"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          287 a          479 c          399 g          335 t
ORIGIN      
        1 tccgttacat tttgggaggc aaccgcccca gttaaactac ccacctgacg ctgttccaga
       61 tccggattca cggaccctgg ttagaagccc agcacgagca gggtggtatt tcaaggttga
      121 ctccaccgag actagcgtcc cagtttcacc gtctcccacc tatcctacac aacccgtacc
      181 agactccaac atcaagctgt agtgaaggtc ccggggtctt tccgtccttc cgcgggtaac
      241 gagcatcttt actcgtattg caatttcacc gagccactgg ttgagacagc gcccaagtcg
      301 ttacgccatt cgtgcaggtc ggaacttcgt cacattcccc tgtttccagg ggagacagac
      361 tatgccttca tctcggtcag gcttccgatg atcaggtcgg cgggataccc acgcttgccg
      421 cttgggttca tctcaccagc cagtcgtacg atctccacga gcccactccg gagcaggtgc
      481 tcgcgtcgag ccatgcgttc acagatgtct gcgaaccgct cgaagtcacg ctgcttcccg
      541 gagagcatcg ggtacttgtt gaagtgaggg atcacggtcg cacggatcgg atcgagactg
      601 cgtgtctccc actttaccgt gcggtcactg cggtccggcc gtatgcgtcc gcatccgaag
      661 taccgctgga tgagcagcag cacttcagca cggtcgccgt tctggctgac cgacagactc
      721 ggcctcacct cccagtaggc cgacagcgtc ggccgaggag agatggagac cgtgaagcat
      781 ccctctccgt cgacgtaccc ggagatgtac gacccaagat caacatcgcc atgccaagag
      841 cccgcgtctt gccccagcca tagatttgcc atgccccgac tgtacgtcgc ggcagtgaca
      901 gggactggag cctctccaca agggatcagt cgttacgggg ccatgccgcc tgagcggcag
      961 agggcttccc acggcatcgc ccgccggcta tcgcctcgtc cttggttcga ggcagccagg
     1021 tagggtttcg ccgttatgag cgggtctgca tatgggatca ctcccatatg gggcaactgt
     1081 tcacccgaca aggaatttcg ctaccttagt accgttatag ttacagcagc cgtttactgg
     1141 cgcttaagtt cggagcttcg ccctgcaagc agagctaacc cctcccctta acgttccagc
     1201 actgggcagg cgtcagtccg tatacggcgt ctttcgactt agcacggacc tgtgttttta
     1261 gtaaacagtc gcttgggcct ggtctctgcg accgccttcc gctcacaccg cgagggtgat
     1321 cacggactgc ggcacccctt atcccgaagt tacggggtca agttgccgag ttccttaacc
     1381 agtgttcgct cgatcgcctt agtattctct actcgcccac ctgcgtcggt ttgcggtacg
     1441 ggctccaact ccactcgcta gaggtttttc ttggcagcgt aggatcatcc acttcgccta
//
LOCUS       DATBRK010000058         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1739010, whole genome shotgun sequence.
ACCESSION   DATBRK010000058 DATBRK010000000
VERSION     DATBRK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739010"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VIH70_01760"
     CDS             <1..853
                     /locus_tag="VIH70_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY8115120.1"
                     /translation="PELESWHVIVDNCAHQLVKRPEQFDVIVTTNMNGDILSDLSSAL
                     IGGLGFAPSANVGNEVAIFEAVHGSAPKYAGKDVINPTAVILSSVLMLRYLGFFEQAA
                     AIEHAVFVTLESGVLTGDVLGYDRGTPTTGYTNAIIGNLGKRTETWTIRDVRPLQLPK
                     IDPRPDYVRPANRSVVGLDVFVESPLSAAELGASLMELTHDTKLSLKMISSRGTKVFP
                     PTGAMTETGDHFRCRFIIKEEPGDLSDGDVHELLVRISSAHSWMHIEKLQEFDGELGF
                     TRDQGED"
     gene            complement(858..1061)
                     /locus_tag="VIH70_01765"
     CDS             complement(858..1061)
                     /locus_tag="VIH70_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115121.1"
                     /translation="MIPQNALQTLRDREVEIRRRSENERRRRAELDRRSDPYGMDAEI
                     RVLIERAEPACCDCEVEVTQEAG"
     gene            1161..>2038
                     /locus_tag="VIH70_01770"
     CDS             1161..>2038
                     /locus_tag="VIH70_01770"
                     /inference="COORDINATES: protein motif:HMM:NF039924.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5937 family protein"
                     /protein_id="HEY8115122.1"
                     /translation="MIRVHLSPEDLAEIRFAFSPTWELVMSAMKALKHPAKHALHLPW
                     VQEARTAIDGRDLGILFALTSGSQYVPDFVTPPPTTPFPELDEELEVVAGTPAERVRI
                     ELDTLVDAIDGHVPEPVRLMAEDPAAWLPRLVDQMREFWKLTIEEHWPRIRAMHEGDV
                     MYRARQLALGGAEPLFGDLHPAVAWRDGVLEIDKPHEADVVPAGSGMILIPAVFDWPG
                     VAVIHDEAYRFTLSYSPRGIANIWEAEPPKSGAMDALIGGTRADILRILEVPMTTSEI
                     ATRLHLTPAAVSQQLGL"
BASE COUNT          332 a          703 c          672 g          331 t
ORIGIN      
        1 cccggagctc gagtcctggc acgtgatcgt cgacaactgc gcgcaccagc tggtcaagcg
       61 gccggagcag ttcgacgtga tcgtcaccac caacatgaac ggcgacatcc tgtcggacct
      121 gtcctcggcg ctgatcggcg ggctggggtt cgcgccctcg gcgaacgtcg gcaacgaggt
      181 ggcgatcttc gaggcggtgc acggatcggc gcccaagtac gccggcaagg acgtgatcaa
      241 cccgacggcg gtgatcctgt catcggtcct gatgctgcgc tacctcgggt tcttcgagca
      301 ggcggcggcg atcgagcacg cggtgttcgt caccctcgag tccggggtgc tcacgggcga
      361 cgtcctcggg tacgaccgcg ggacgccgac gacgggctac acgaacgcga tcatcggcaa
      421 cctcggcaag cgcaccgaga cctggaccat ccgcgacgtc aggccgctgc agctgccgaa
      481 gatcgacccg cgcccggact acgtccggcc ggcgaaccgt tccgtggtcg gcctggacgt
      541 cttcgtcgag tcgccgctct cggccgccga gctcggcgcg tcgctgatgg agctcacgca
      601 cgacacgaag ctgtcgctca agatgatctc gagccgcggc acgaaggtgt tcccacccac
      661 cggcgcgatg accgagaccg gcgaccactt ccgctgccgc ttcatcatca aggaggagcc
      721 gggcgacctg tccgacgggg acgtccacga gctgcttgtg cggatctcga gcgcgcactc
      781 gtggatgcac atcgagaagc tccaggagtt cgacggcgag ctggggttca cgcgcgacca
      841 gggcgaggac tgagggttta tccggcctcc tgcgtgacct ccacctcgca gtcgcagcag
      901 gccggctcgg cgcgttcgat caggacacgg atctcagcgt ccatgccgta ggggtcgctg
      961 cggcgatcca gctcggcgcg tcgccgccgc tcgttctcgg atctcctgcg gatctcgacc
     1021 tcacggtccc tcagcgtctg gagcgcgttc tgggggatca tcgctctctc cttgatcgcg
     1081 ggcttgcgca cacgatagtg cggaagctac aatcgctgaa gtatttcagg aatcccttaa
     1141 tcttaggtaa tggagcaggc atgattcggg tgcacctctc tcccgaggac ctggccgaga
     1201 tccgcttcgc cttttctccc acgtgggagc tcgtgatgag cgcgatgaag gcgctcaaac
     1261 acccagccaa acacgccctc cacctgccct gggtccagga ggcgcggacc gcgatcgacg
     1321 ggcgggatct cgggatcctg ttcgccctca cgtcggggtc gcagtacgtg cccgacttcg
     1381 tgaccccgcc cccgaccaca ccgttcccgg agctcgacga ggaactcgag gtcgtggccg
     1441 gcacgcccgc ggaacgggtc cggatcgagc tcgacacgct cgtcgacgcc atcgacggcc
     1501 acgtccccga gcccgttcgg ctgatggccg aggatccggc ggcgtggctc ccgagactcg
     1561 tcgaccagat gcgggagttc tggaagctga cgatcgagga acactggccc cgcatccgag
     1621 cgatgcacga gggcgacgtc atgtaccgcg cccggcagct cgccctgggc ggagccgagc
     1681 cgttgttcgg cgatctccat cccgcggtcg cgtggcgaga cggggtgcta gaaatcgaca
     1741 agccccacga ggccgacgtc gtgccggccg gctccgggat gatcctgatc ccggcggtgt
     1801 tcgactggcc cggcgtcgcg gtgatccacg acgaggccta ccggttcacg ctgtcctact
     1861 cgccccgagg gatcgctaac atctgggagg ccgagccacc gaagtcgggg gcgatggacg
     1921 ctctgatcgg cggcacgcgc gccgacatcc tgcggatcct cgaggtcccc atgacgacct
     1981 cggagatcgc gacccgcctc cacctgacgc ccgcggcggt gtcacagcag ctcggcct
//
LOCUS       DATBRK010000059        18863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_585127, whole genome shotgun sequence.
ACCESSION   DATBRK010000059 DATBRK010000000
VERSION     DATBRK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18863
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585127"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VIH70_01775"
     CDS             <1..1201
                     /locus_tag="VIH70_01775"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEY8115123.1"
                     /translation="ADFPILERTSHGKRLVYLDSANTSQKPRAVIDTITEYYERHNAN
                     LYRAVYELAEEATAMFEGARTRLASFIGAPDPSTVVFTRGTTESINLIANAWGRRFLH
                     ERDEILFSELEHHSNIVPWQLVAEATGAVTRLIPIEDDGTLDAARIEGMLTDRTKVLA
                     ISGQSNVLGTMPAIGRLAESAHAAGAIVVVDGAQLVPHNPVDVAALDIDFLTISGHKM
                     LGPTASGGLYGRLELLEAMSPFLGGGEMIMEVYPDHSTYKAPPHKFEAGTMNIAEEIG
                     LATAIDYLEGLGMENVRSHERELTAYAIETLGSIGATVYGPKVPEQRGGAVSFWYKDI
                     HPHDLAQVLDQEGVCVRPGHHCAQVLMRRLGVPATTRASFYVYNTKEDVDALIAALEK
                     AGEFFGA"
     gene            1191..1640
                     /locus_tag="VIH70_01780"
     CDS             1191..1640
                     /locus_tag="VIH70_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY8115124.1"
                     /translation="MALDELYKEVILDHYKNPRNKREIPDAALRCSKNNPLCGDEITV
                     FVNEDDGTISDVAFVGQGCSISQSSASMMTEAAAGKTRQEVEQRIGAFRGMMAGEVEA
                     DEDAFGELVALKGVVKYPVRVKCAVLAWDVLQEALAGEGEAGGSAPH"
     gene            complement(1641..2384)
                     /locus_tag="VIH70_01785"
     CDS             complement(1641..2384)
                     /locus_tag="VIH70_01785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8115125.1"
                     /translation="MEAGTPKLYREYAAWWPLLSAPEEYAEEATEYARLILGASNGEA
                     HTVLELGSGGGNNASHMKGSFDLTLVDRSPQMLEVSRGLNPECEHIEGDMRSVRLGRA
                     FDAVFVHDAIAYMTTEDDLRGVFATAFEHLRPGGVALFVPDALTETFEEAADHGGHDG
                     TDRALRYLEWTWDPDPDDTTVRMDLTYVFHEPDGSVHVELDEHVCGLFPRATWLGLIE
                     GTGFEVRAVPLTHDERPVGAEGFLAVRPR"
     gene            2446..3507
                     /gene="cax"
                     /locus_tag="VIH70_01790"
     CDS             2446..3507
                     /gene="cax"
                     /locus_tag="VIH70_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEY8115126.1"
                     /translation="MPRARPSLDWLLVLVPVSIVLEVAGGNELLIFLTSAGAILPLAG
                     LIGRSTEQLALHTGPRVGGLINATFGNVTELVIAFFLIMDDKTEIVKASLTGSIIGNL
                     LLVLGLSFLVGGLKHEEQTYNARAASVHATSLVLAVTGLLMPALFAHGAGNETFAQRE
                     VVSATVAAVLMALYAAALIFTLVTHEHLFRTPSPEEHPQWSRRQAIGVLLASTAVVAL
                     EAEFLVGSLEPALADLGLSELFVGLIVIPIIGNAAEHSSAILFALRDKVDVTLEIAIG
                     SSTQIALFVAPALVFISLLVGHPMDFVFSTFEVAAVALSTVLVFMISTDGRSNWLEGA
                     QLTGAYVIMAISFLFVETL"
     gene            3522..5315
                     /locus_tag="VIH70_01795"
     CDS             3522..5315
                     /locus_tag="VIH70_01795"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEY8115127.1"
                     /translation="MAWPAVAFMILVPAAPARGQETVFESTPRFDVNIVVEQSGDLEI
                     TETIVQEFGSTPRHGIIRFIPNRLRYDDEFDRVYPIELVSVQTSPNTPADVETNEENG
                     NFVIRIGDPDTTITGRHTYEIVYRVMGAMNGFADHDELFWNAIGDQWDQPIGEMNVRV
                     SGPTDVTRVACYRGAFGSTTRCDHAQITRKGDAVFSQSNLPAFQAMSVVVALPQGTVA
                     STAPILDERWSLDRAFARTPASVGGGVGLLALVVAGFGTLMWKRGRDVRFRGSQVDQA
                     MGGPPGAEMQAVPLFEKGTSVVEFAPPEDLRPGQVGTLVDEVANTLDVSATIVDLAVR
                     KYLVIEEIPKTWLLGKADWNLRLLPEPADDELLPYERKLLDGLFEDGDQVELSTLRKT
                     FAERLQTVKDALYDDMVSRRWFLRRPDRVRQTWVVIGSLAMSVGIAITILLAVFTKYG
                     LLGIPFVIGGILLLIGANRMPARTAKGTAMTRRVNGFRIVIEKAEAYMSKWAEQENVF
                     TRFLPYAVVFGVTDKWAKAFESLGQLPSDTTWYVSSRPFVYAQFADSIDSFTVQTSGT
                     ISSTPAGSGSSGFGGGGGAGGGGGGGGGGSW"
     gene            5357..6349
                     /locus_tag="VIH70_01800"
     CDS             5357..6349
                     /locus_tag="VIH70_01800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8115128.1"
                     /translation="MDIVFSQDPRDFARRDWSALVTADPAGTVFHTPAFLKLYWEEFA
                     DLPDHLLLAFAEEGGDQVGAVAFERLDGTLRFLGGTEVTDYMGPVGLPEARPTVAKEL
                     FAALARTDNWIEADLRGLPEDQPWLGLLVEAAAAQGFSTEVLDDQNGIAPLLALPGSY
                     AEYLERLPAKLRHEIKRKARRLEAEVGPWHICLATPETLEPFLDRFVELHRTSEGPKG
                     VFMQPGMEIFFRRLGDAFLPRGIFSLTFIETSEHLKLAGSIGFRFEGTTSLYNSAFDR
                     AYQPVSPGMVLVAEDIRIAIEAGCSAFDMLKGDYAYKYRFGAIPRSIKRLVVTR"
     gene            complement(6364..7194)
                     /locus_tag="VIH70_01805"
     CDS             complement(6364..7194)
                     /locus_tag="VIH70_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEY8115129.1"
                     /translation="MTLDRTRLLGIAHAERERLGRTIQYTSPDAWDAPSVCQGWRNRD
                     IVAHLAAQDTAAAQLLAEDRAAEFDAFREANDGELWVNGFNEWAVEIREEVPTRQLIT
                     DWGKAAQVFLVLCSGLTDDEWAAKKVEWVAGEIGLRYLVQSRTIEWWFHQEDIREGAG
                     LEGNPQHDPVYLTNDMAIRMLPWALEQAELSFPGRSIRVDLEGVGGGTWHWGLEPRTT
                     PPADKKPDAFINGRGTAFALVAGRRVAAESYLDDGDLVVGGDETLAIVVLEVLRAFVE
                     "
     gene            7341..7844
                     /locus_tag="VIH70_01810"
     CDS             7341..7844
                     /locus_tag="VIH70_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017547064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY8115130.1"
                     /translation="MSKAIERFLDAATVLGHTVDVRRFPEGTRTAPDAARAIGCEVGQ
                     IVKSLVFVADGEPLLALTSGANRVDLGRLATRAGASAARRADPEEARAATGFAVGGTP
                     PLGYPKPIRTFLDPDLLVFEEVWAAAGTPDAVFRTTAAELQRVTAAEVVDLKETASRD
                     ENVPPNR"
     gene            7886..8206
                     /locus_tag="VIH70_01815"
     CDS             7886..8206
                     /locus_tag="VIH70_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018020336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY8115131.1"
                     /translation="MIQLTESAAGKVKELLVEEGRSDIALRVAVQPGGCSGLRYAMYL
                     DDQLTEKDVAEEQFGVRVVIDRMSVPYLSEAKIDFVDSLEASGFTIDNPVAQGSCACG
                     HSFH"
     gene            complement(8249..9466)
                     /locus_tag="VIH70_01820"
     CDS             complement(8249..9466)
                     /locus_tag="VIH70_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8115132.1"
                     /translation="MAAVESAGELVIDRGEDAHVWDEHGRRYLDATAALWYCNVGYGR
                     EEIADAAHRQMMRLSAYSTFGDQSNRPAIELAERVGRLAPVPDSKVFLTSGGSDSIET
                     ATKMARRYWQLRGETERTLLVTRQHAYHGMHVAGTSLAGIEANAAGHGPLVGDVVKIA
                     WDSPAALREAIAAAGPERVAAFFCEPVIGAGGVFASPEGYLAEVRAICRETGVLFVAD
                     EVITGFGRVGDWFASSRWDLEPDILTCAKGITSGYLPMGAVIAAPWVAEPFWRPGAGL
                     WRHGYTYSGHATAAAAAIANLDVMEREELPKRALELERDLVTALAPLADHPLVGEVRA
                     GTGVLAAVQFAADALEADPGLPARAVTACRANGILTRALGIGALQVSPALVLSQGELD
                     ELRDGLRGALDAA"
     gene            complement(9551..13174)
                     /gene="metH"
                     /locus_tag="VIH70_01825"
     CDS             complement(9551..13174)
                     /gene="metH"
                     /locus_tag="VIH70_01825"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY8115133.1"
                     /translation="MRERLEALLAERILVLDGAYGTAIQARSLADGDYRGENFRDHPR
                     DVAGDPDLLNLTRPDVVEDIHRSYLAAGADIVTTNTFTATPIAQADYGLEEAVYDLNL
                     AGARIARRAAEAFGPDRFVAGSVGPTNQTLSLSPRVNEPAYRTVSFDRMAEAYAEQIR
                     GLVDGGIDLLLIETIFDTLNAKAAIAAASDVAPGVPLMISVTITDRSGRTLSGQTVDA
                     FWLSIAHAEPLTVGVNCALGAAEMRPYLETLARIAPVYTTCHPNAGLPNAFGGYDERP
                     ETTSKYLREFAESGLANVLGGCCGTTDEHVRAIAAAVDGLSPRRVPAQRTATSFSGLE
                     PFSITPETGFVMIGERQNVTGSARFRRLIEVGDYQAAVEIAADQVRSGANMLDVNMDA
                     DLLDGVKAMTTFLNLIATEPEIARVPVMVDSSKWDVLEAGLKCLQGKGIVNSISLKEG
                     EEEFLEKARRVKRYGAAVVVMAFDEQGQADTVERKLQILERSVGLLIDRAGYVPEDIV
                     VDPCILAIATGIEEHNAYAVAFIEATRRLKERLPGVKVSGGVSNLSFSFRGNDAVREA
                     IHSAFLYHAIAAGLDMAIVNAGQIALYEDIPADLLEHVEDIIFDRRPDATERMVSFAE
                     TVRGGAMKKELDLSWRETTVEERLKHALVHGIDEFIEQDTEEARQAHERPLDVIEGPL
                     MAGMQVVGDLFGAGKMFLPQVVKSARAMKRAVAYLEPFMEQEKLLAGPEGVRAQGKVI
                     LCTVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVPPDVLLDTAAAEGCDIVGCSGLIT
                     PSLGEMEGVAKEMERRGLELPLLIGGATTSRQHTAVRIAPEYGQPVVHVVDASRVVGV
                     VSDLLDPDRRSKLDHVNREDQERLRLLHAEREAQPLLPYRIATERRTPIVWRQQDLPE
                     PAFTGARVVEPDLAELRPYIDWTFFFGAWELKGRFPQILDHPRHGEAARELFEAANEL
                     LDRIVAERSLAARGVYGFWPASAEGDDVVLSGGPRFPMLRQQADHGDDRPSRSLADYV
                     APAETGLVDHIGAFAVTAGLGADELAAQFETDLDDYQAIMVKALADRLAEAFAEWLHE
                     RARREWYESGPEMSGDDLLAERYRGIRPAFGYPACPDHTRKATLFELLDAPSAGIDLT
                     EHFAMTPAASVSGLYFAHPAARYFNVGRLGRDQVEEYAARCGMDRREAETWLRPNLAY
                     EPS"
     gene            13193..13822
                     /locus_tag="VIH70_01830"
     CDS             13193..13822
                     /locus_tag="VIH70_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8115134.1"
                     /translation="MPRCDQHARSSFRRGHHVGYAARSGFVEPVEEAALGKDAFEPLT
                     RAVPGAIATGGIGDSAPSGRRRPDRPLGRRGADLLAQVPLFEGLSRRHLKQIAERADE
                     ISFRQGETIVEADQPGGSFFVIVEGEVRVVRGDRTIARAGPGEFFGEISLLDGGPRTA
                     SVIAETPVVAIRLFKASFDKIVREEPHVAGKILVVVARRLREAEKTVTA"
     gene            13822..14313
                     /locus_tag="VIH70_01835"
     CDS             13822..14313
                     /locus_tag="VIH70_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY8115135.1"
                     /translation="MLAFTAYTWWKFLHVAGVIAFVMFHGVSMTVALQLRRERDRGRI
                     ATITQLSGSSLRGMYVALLWLIVFGVIAGIQADWWNDGWFWISVGLLVVAIAEMSAVA
                     RPYYERVKEAIEIRPSGVPRRSDEELDEILRSPIGLWNTVFGVAVLAVIAWLMIFKPF
                     VVL"
     gene            complement(14321..15346)
                     /locus_tag="VIH70_01840"
     CDS             complement(14321..15346)
                     /locus_tag="VIH70_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8115136.1"
                     /translation="MYRRIVVATDGSDSAETAETIASTLASIGGAKLTIAHAYANPDR
                     ANEAVARAKEIADREGARHELALSAEAPADAILMTADEKDAELIVIGSVGFSTSDQLF
                     GSVSRRVVTHAPCDVLLTRARTIGEHEKPAPPYRRMLIATDGSSTADRAARKGYVLAK
                     ALKASVTLLFVGHPKTGELVLQDTVATIGSESGVTSAIEIRSGDPSDEIVDAAATEGF
                     DLVVIGNRGMTGAKAAMLGSVPRDVAESAPCDVLVARTVAQNLDEIGPDEGGIVKMAD
                     HKVAVYRDKKGNLTILSATCTHQGCTVKWDASEHGWLCPCHGSRYSATGQVVAGPAER
                     PLAPTDL"
     gene            complement(15366..16313)
                     /locus_tag="VIH70_01845"
     CDS             complement(15366..16313)
                     /locus_tag="VIH70_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8115137.1"
                     /translation="MRRNRLGSRGPELSAIGFGAWEAGGGSEWGDPPPEEQVLRAIRT
                     VFETGIDRIDTAEVYGGGKSEELVARAIAGRRDEVLIATKVAPQPEGSGFRAAHVRSA
                     CEGSLRRLGTDHVDLYQLHWPDERGVPLEETWNAMAALLDDGLVRSIGVSNFDRALIE
                     RCEAIRHVDSLQQEFSMLRLEDRDLIAWCGEQGTGVLSYAPLAYGLLTGAITTETRFD
                     EGDFRAGEDEGGSLFARGNRERNLAAVDALRPIARRLGITLAQLALAWNAAQPGVTAA
                     IAGSRNPDHVRSNAAAGEVELDEATLSELEAVLSRTPPG"
     gene            16478..18355
                     /locus_tag="VIH70_01850"
     CDS             16478..18355
                     /locus_tag="VIH70_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017610228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY8115138.1"
                     /translation="MTKQVEEKQRVVIRFAGDSGDGMQLTGDRFTNATAVLGNDLATM
                     PDFPAEIRAPAGTVHGVSAFQIQFASTDILTPGDHPNVLVAMNPAALKAELGSVEKGG
                     TIIVNEDAFTPRNLQKAGYASDPLTDGLLDAYQIYRVPMTSITVRAVEELGLTRKEAE
                     RSKNMFALGLVSWMYGRPTDVTLNWLHKKFGDKPQLYDANAAAFKAGYNFGETTELFA
                     TSYEVKPAPAEPGVYRNVSGAQALAWGLVAAAERSGLQLFYASYPITPASELLHELSR
                     LKNFSVMTLQAEDEIAAANVALGAAFAGDLGVTGTSGPGMDLKAETLGLAVMLELPMV
                     IVDVQRAGPSTGMPTKTEQADLLLAMFGRHGESPMPIVAARSSSHCFDAAIEAARIAI
                     TYRTPVILLSDTFLANSSEPWRLPDVESLPTIDPAFATGPNAGQEFLPYLRDEKLARP
                     WALPGTPGLRHRIGGLEKEDGTGDISYEPENHERMTQLRAAKVAKIADDIPPLEVDDP
                     HGDAELLVLGWGSTYGTILAAARRVRERGKKVATAHLVHLNPMPANTGGIVKRYAKVL
                     IPELNTGQLAMLIRSEHLVDAKTFSKVQGQPIFAEELEDEILRVIGEETRVGGDKVMD
                     R"
     gene            18352..>18863
                     /locus_tag="VIH70_01855"
     CDS             18352..>18863
                     /locus_tag="VIH70_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8115139.1"
                     /translation="MSDSGNGTTNGDRAIGNGDGAVGTLTKKAFTSDQEVRWCPGCGD
                     YAILSTVQGFMPELGIEPDRTVFVSGIGCSGRFTYYMDTYGVHGIHGRAPALATGIAT
                     ARPDLSVWVITGDGDGLSIGGNHLIHALRRNVNLKILLFNNQIYGLTKGQYSPTSEEG
                     KVTKSTPFGSV"
BASE COUNT         2830 a         6524 c         6565 g         2944 t
ORIGIN      
        1 ggccgacttc ccgatcctcg agcggaccag ccacggcaaa cgcctggtct acctggacag
       61 cgccaacacc agccagaagc cgcgcgcggt catcgacacg atcaccgagt actacgagcg
      121 gcacaacgcg aacctgtacc gagcggtcta cgagttggcc gaggaggcca cggccatgtt
      181 cgagggcgcc cggaccaggc tggcgagctt catcggcgcg ccggatccct cgacggtcgt
      241 cttcacgcga ggcaccaccg agtcgatcaa cctcatcgcc aacgcctggg gccgcaggtt
      301 cctccacgag cgcgacgaga tcctgttcag cgagctcgag caccactcga acatcgtgcc
      361 ctggcagctc gtcgccgagg cgaccggggc cgtcacgcgg ctgatcccga tcgaggacga
      421 cggcacgctg gacgccgcga ggatcgaggg catgctcaca gaccggacca aggtgctcgc
      481 gatcagcggt cagtccaatg tgctgggcac gatgccggcg atcggccggc tcgcggagtc
      541 cgcgcacgcg gccggcgcga tcgtcgtcgt cgacggcgcc cagctggtcc cccacaatcc
      601 cgtcgacgtc gccgccctgg acatcgactt cctgacgatc tcgggccaca agatgctcgg
      661 cccgaccgcc tccggcggcc tgtacgggcg cctcgagctc ctggaggcga tgtcgccctt
      721 cctcgggggc ggcgagatga tcatggaggt ctaccccgac cactcgacct acaaggcgcc
      781 gccgcacaag ttcgaggccg ggacgatgaa catcgccgag gagatcgggc tcgcgaccgc
      841 gatcgactac ctcgagggcc tcgggatgga gaacgttcga agccacgagc gggaactcac
      901 cgcgtacgcg atcgagacgc tcggatcgat cggcgcgacg gtctacgggc cgaaggtccc
      961 cgagcagcgc ggcggagcgg tctccttttg gtacaaggac atccatccgc acgacctggc
     1021 ccaggtgctg gaccaggagg gggtgtgtgt gcggcccgga catcactgcg ctcaggtcct
     1081 gatgcggcgg ctgggggtgc cggcgacgac gcgggcctcg ttctacgtgt acaacacgaa
     1141 ggaggacgtg gacgcgctga tcgcggcact ggagaaggcg ggggagttct ttggcgcttg
     1201 acgagctcta caaagaggtc atcctcgacc actacaagaa cccgcggaac aagcgggaga
     1261 tccccgacgc ggcgctgcgt tgctcgaaga acaacccgct gtgcggcgac gagatcacgg
     1321 tgttcgtcaa cgaggacgac ggcacgatct cggacgttgc gttcgtcggc caggggtgct
     1381 cgatcagcca gagctcggcc tcgatgatga ccgaggccgc tgccggcaag acgcgccagg
     1441 aggtcgagca acggatcggc gccttccgcg gcatgatggc cggcgaggtc gaggcggacg
     1501 aggacgcgtt cggcgagctc gtggcgctca agggcgtcgt gaagtacccg gtccgcgtga
     1561 agtgcgccgt gctcgcgtgg gacgtgctgc aggaggcgct cgccggggag ggcgaggccg
     1621 ggggttcggc cccgcactga ctaccggggg cgaaccgcta ggaagccctc ggccccgacc
     1681 ggacgctcgt cgtgggtcag cggcacggcc cgcacctcga acccggtccc ctcgatcagc
     1741 ccgagccacg tcgcccgggg gaacagcccg cacacgtgct cgtcgagctc gacgtggacg
     1801 gacccgtcgg gctcgtggaa cacgtacgtg agatccatcc gcaccgtcgt gtcgtccggg
     1861 tccggatccc aggtccactc gaggtagcgc agtgctcggt ccgtcccgtc gtgcccgccg
     1921 tggtccgccg cctcctcgaa ggtctcggtc agtgcgtcgg gaacgaacag cgctacgcca
     1981 ccgggacgca ggtgctcgaa cgccgtcgcg aacaccccac gcagatcgtc ctcggtcgtc
     2041 atgtacgcga tcgcgtcgtg cacgaacacc gcgtcgaacg cccgccccag ccgcaccgaa
     2101 cgcatgtcgc cctcgatgtg ctcgcattcg gggttgagcc cgcggctcac ctcgagcatc
     2161 tgcggcgagc ggtcgacgag ggtcaggtcg aacgaccctt tcatgtgcga agcgttgttg
     2221 ccgccgccgg agccgagctc gaggaccgtg tgggcctcgc cgttcgacgc gccgaggatc
     2281 agccgtgcgt actcggtggc ctcctcggcg tattcctccg gcgccgacag cagcggccac
     2341 cacgccgcgt actctcggta gagcttcggc gtccccgctt ccatcacggc gtcagcctag
     2401 cggtcgctca ccgcacgcca ccctccgacc gcataggctt cgcgcgtgcc gagagctcga
     2461 ccctcgctcg actggctgct cgtcctcgtc cccgtttcca tcgtgctcga ggtggccggc
     2521 ggcaacgagc tcctgatctt cctgacctcg gccggtgcga tcctgccgct ggcggggctg
     2581 atcgggcgct ccaccgagca gctggccctg cacacgggtc cgcgtgtagg tgggctgatc
     2641 aacgcgacgt tcggaaacgt gacagagctg gtgatcgcgt tcttcctgat catggacgac
     2701 aagaccgaga tcgtgaaggc gtcgctcacc ggctcgatca tcgggaactt gctgctggtc
     2761 ctcgggctgt cgttcctggt gggcggactc aaacacgagg agcagaccta caacgcgcgc
     2821 gcggcgtccg tgcatgccac ctcgttggtg ctcgcggtca ccggcctcct catgcccgcg
     2881 ttgttcgccc acggggcggg caacgagacg ttcgcccagc gcgaggtggt gagcgccacc
     2941 gtggccgcgg tcctgatggc gctgtacgcg gccgccctga tcttcacgct ggtcacccac
     3001 gagcacctgt tccgaacgcc gtcgcccgag gagcatccac agtggtctcg gcggcaagcc
     3061 atcggggtcc tgcttgcctc caccgccgtg gtcgcgctcg aggccgagtt cctggtgggt
     3121 tcgctcgagc cggcgttggc ggacctcggc ctgtccgagc tcttcgtggg cctgatcgtg
     3181 atccccatca tcggcaatgc ggcggagcac tcgtcggcga tcctgttcgc cctgcgggac
     3241 aaggtggacg tcacgctcga gatcgccatc ggctcctcga cccagatcgc cctgttcgtg
     3301 gcgccagccc tcgtgttcat cagcctgctg gtgggccacc cgatggactt cgtgttcagc
     3361 acgttcgagg tggcggccgt ggcgctgtcg accgtcctgg tcttcatgat ctcgacggac
     3421 ggccggagca actggctgga gggcgctcag ctcacgggcg cgtacgtcat catggccatc
     3481 tcgttcctct tcgtggagac gctgtgagac ggttcgtccg attggcttgg ccggcagtgg
     3541 cgttcatgat cctggtgccg gccgcgccgg cgcgcgggca ggagaccgtt ttcgagtcca
     3601 ccccgcggtt cgacgtgaac atcgtggtcg agcagtccgg cgacctggag atcaccgaga
     3661 cgatcgtgca ggagttcgga tcgacgcccc ggcacgggat catccggttc atcccgaacc
     3721 ggcttcgcta cgacgacgag ttcgatcgcg tgtacccgat cgagctcgtg tcggtgcaaa
     3781 cctcaccgaa cacgccggcc gacgtcgaga ccaacgagga gaacggcaac ttcgtgatcc
     3841 ggatcggcga cccggacacg acgatcaccg ggcgccacac ctacgagatc gtctaccggg
     3901 tcatgggcgc gatgaatggg ttcgccgacc acgatgagct gttctggaac gcgatcggcg
     3961 accagtggga ccagccgatc ggcgaaatga acgtccgcgt gagcggcccc acggatgtca
     4021 cgcgggtcgc ctgctatcgg ggcgcgttcg ggtcgacgac ccgctgcgac cacgcccaga
     4081 tcacgaggaa gggagatgcg gtcttctcgc aatcgaacct gcccgcgttc caggcgatgt
     4141 cggtcgtggt cgcgctcccg cagggcaccg tcgccagtac cgcgccgatc ctggacgagc
     4201 gctggtcgct cgacagggcg ttcgcccgca cgccggccag cgtgggcgga ggcgtcgggc
     4261 tgctcgccct cgtcgtcgcg gggttcggca cgctgatgtg gaaacgtgga cgcgacgtcc
     4321 gattccgcgg ctcacaggtc gaccaggcga tgggcggccc gcccggcgcc gagatgcagg
     4381 ctgtcccgct cttcgagaag ggaacctcgg tggtggagtt cgcgccgccc gaggacctgc
     4441 gccccggtca ggtggggacg ctggtcgacg aggtggccaa cacgctggac gtgagcgcca
     4501 ccatcgtgga cctcgccgtg cgcaagtacc tggtgatcga ggagatcccc aagacgtggc
     4561 tgctcggcaa ggccgattgg aacctgcgcc tgctgcccga accggcggac gacgagctcc
     4621 tgccgtacga gaggaagctg ctggacgggc tgttcgagga cggcgaccag gtcgagctgt
     4681 cgacgcttcg caagacgttc gccgagcgcc tgcagacggt caaggacgcg ctctacgacg
     4741 acatggtctc ccgcaggtgg ttcctgcggc gccccgaccg cgttcggcag acgtgggtcg
     4801 tgatcgggtc gctcgcgatg tcggtcggga tagcgatcac gatcctgctc gccgtcttca
     4861 cgaaatacgg cctgctcggc atcccgttcg tgatcggcgg gatcctcctg ctgatcggcg
     4921 ccaacaggat gccggcgcgg acggccaagg gcaccgcgat gacgcggcgc gtgaacgggt
     4981 tccgcatcgt gatcgagaag gccgaggcgt acatgtcgaa atgggccgag caggagaacg
     5041 tgttcacgcg attcctgccg tacgcggtcg tgttcggggt caccgacaag tgggcgaagg
     5101 cgttcgagtc gctcgggcag ctcccctccg acacgacctg gtacgtgagc agccgcccgt
     5161 tcgtgtacgc gcagttcgcc gacagcatcg actcgttcac ggtgcagacc agcgggacga
     5221 tctcgtcgac acccgccggc tccggctcca gcgggttcgg gggtggcggc ggggccgggg
     5281 gaggaggcgg cggcgggggc gggggctcct ggtagggtcg ccgcgagacc ggtgatgagc
     5341 gcggggctcg cacgcgatgg acatcgtctt cagccaggac ccgcgggact tcgcccggcg
     5401 tgactggtcg gcgctcgtca cggccgaccc ggccggcacc gtcttccaca cgcccgcctt
     5461 cctcaagctg tactgggagg agttcgccga cctgccggac cacctgttgc tcgcgttcgc
     5521 ggaggagggc ggggaccagg tcggcgcggt cgcgttcgag cgcctcgacg ggacgctgcg
     5581 gtttctcggc gggaccgagg tgaccgacta catgggaccg gtgggactgc ccgaggctcg
     5641 gcccaccgtc gcgaaggagc tgttcgcagc gctcgcgcgt acggacaact ggatcgaggc
     5701 cgatctccgc ggcctcccag aggaccagcc ctggctcggc ctgctcgtcg aggcggctgc
     5761 ggcgcaaggg ttctcgaccg aggtcctcga cgatcagaac gggatcgcgc ccttgctcgc
     5821 cttgcccggt tcgtacgcgg agtacctcga gcgtctcccg gccaaactgc gccacgagat
     5881 caagcgcaag gcgcgtcgcc tggaggcgga ggtcgggccc tggcacatct gcctggcgac
     5941 cccggagacc ctcgagccct tcctcgatcg cttcgtcgag ctgcaccgca cgagcgaggg
     6001 gcccaagggc gtcttcatgc agccggggat ggagatcttc ttccgcaggc tcggcgacgc
     6061 gttcctccca cgagggatct tcagcctgac gttcatcgag acctcggagc atctgaagct
     6121 cgcgggttcg atcggcttcc ggttcgaagg caccacctcg ctgtacaact ctgcgttcga
     6181 ccgcgcctat cagcccgtgt cgcccgggat ggtgctcgtg gccgaggaca tccgcatcgc
     6241 gatcgaggcg gggtgttcgg cgttcgacat gttgaagggc gactatgcgt acaagtaccg
     6301 gttcggggcg atcccgcggt cgatcaagcg gctggtggtg acgcgctagc cgcgcgacgg
     6361 gggctactcg acgaacgcgc gcagcacctc gagcacgacg attgcgagcg tctcgtcccc
     6421 gcccacgacc aggtcgccgt cgtcgaggta ggactcggcg gccacccgtc ggccggccac
     6481 cagcgcgaac gcggtgccgc ggccgttgat gaacgcgtcg ggcttcttgt ccgccggagg
     6541 cgtcgtccgc ggttcgaggc cccagtgcca cgtgcccccg ccgacgccct ccaggtccac
     6601 ccggatgctc cggcccggga acgacaactc ggcctgctcc agcgcccagg gcagcatccg
     6661 gatcgccatg tcgttcgtca ggtagaccgg gtcgtgctgc gggttgccct ccagacccgc
     6721 gccctcgcgg atgtcctcct ggtggaacca ccattcgatc gtgcgggact gcacgaggta
     6781 ccgcaggccg atctcgccgg cgacccactc cactttcttc gccgcccact cgtcgtcggt
     6841 gagtcccgag cacagaacga ggaacacctg ggccgccttg ccccaatcgg tgatcagctg
     6901 acgggtcggg acctcctcgc gaatctcgac ggcccactcg ttgaagccgt tcacccatag
     6961 ctcgccgtcg ttggcttccc ggaacgcgtc gaactccgcc gcgcggtcct cggcgaggag
     7021 ctgggctgcc gccgtgtcct gcgcggcgag gtgggcgacg atgtcccggt tccgccaccc
     7081 ctggcagacg ctcggggcgt cccaggcgtc cggggacgtg tattggatcg tcctgccgag
     7141 gcgttcgcgc tcggcgtggg cgatgccgag cagccgggtg cggtccaacg tcacctgttc
     7201 gctccctctc cgtcttcatt ggctgggtgt gctggccgct tccccggcct cggtcggcgg
     7261 gatccgcggg ggatcgagac ccgctcctcg tcgtgcgggc cgtatcgtac ccgccgacct
     7321 gcggccggta tcgttgccgc atgagcaagg cgatcgagcg gttcctcgac gccgccacgg
     7381 tgctcgggca caccgtcgac gtccggcggt tcccggaggg gacgaggacc gcccccgacg
     7441 cggctcgggc gatcgggtgc gaggtggggc agatcgtgaa gtcgctcgtg ttcgtcgcgg
     7501 acggcgaacc ccttctcgcg ctcacgagcg gggccaaccg ggtcgacctc ggccgcctcg
     7561 cgacccgtgc gggcgcctcg gcggcacggc gcgcggaccc ggaggaggcc cgggccgcca
     7621 cggggttcgc ggtgggcggg acgcctccgt tgggttaccc caaacccatc cggacgttcc
     7681 tggaccccga cctgttggtg ttcgaagagg tctgggcggc ggcggggact ccggacgcgg
     7741 tgttccggac cacggcggcc gagctccaac gagtcaccgc ggccgaggtc gtcgatctga
     7801 aggaaacggc ctcgcgggac gagaacgtcc cgccgaaccg ttagaatctt cgggtgagca
     7861 tcacaccgtc tcccacggag gaacgatgat ccagctgacc gaatccgcag ccggcaaggt
     7921 caaggagctc ctggtcgagg agggacgctc ggacatcgcg ctgcgggttg ccgtccagcc
     7981 cggcgggtgc tcgggccttc ggtacgcgat gtacctggac gaccagctca cggagaagga
     8041 cgttgccgag gagcagttcg gggtccgcgt cgtgatcgac cgcatgagcg tgccctacct
     8101 gtccgaggcc aagatcgact tcgtggactc gctcgaggcc tcggggttca cgatcgacaa
     8161 cccggtcgcc cagggctcct gcgcctgcgg gcactcgttc cactagctcg tttccggctc
     8221 ggctcccagg cccgaacccg aacgtcgttc aggccgcgtc gagcgcgccc cgtaggccgt
     8281 cgcggagctc gtccagctca ccctgcgaca ggacgagcgc gggcgagacc tggagcgcgc
     8341 cgatccccag cgcccgcgtc aggatcccgt tcgccctgca ggccgtgacc gcacgggcgg
     8401 gcagacccgg gtcggcctcg agggcatcag cggcgaactg cacggccgcg aggacgccgg
     8461 tgccggcgcg gacctcgccg accagcggat gatcggcgag cggcgccagg gccgtgacga
     8521 ggtcgcgctc gagctcgagc gcccgcttcg gcagctcctc gcgctccatc acgtcgaggt
     8581 tcgcgatcgc cgccgccgcc gccgtggcgt gacccgaata cgtatagccg tgccgccaca
     8641 gcccggcgcc gggacgccag aagggttccg cgacccacgg cgccgcgatc accgccccca
     8701 tcggcaggta gccgctcgtg atgcccttcg cgcacgtcag gatgtcgggc tcgaggtccc
     8761 accgcgaact cgcgaaccag tcgccgacgc gcccgaaccc ggtgatcacc tcgtccgcga
     8821 cgaagagcac gccggtctcg cggcagatcg cgcggacctc ggccaggtac ccctccggcg
     8881 acgcaaacac gcccccggcg ccgatcaccg gttcgcagaa gaacgcggcc acccgctcgg
     8941 gaccggcggc cgcgatcgct tcgcggagcg ccgcagggga gtcccaggcg atcttcacca
     9001 catcgccgac cagcggaccg tggcccgccg cgttcgcctc gatgccggcg aggctcgtcc
     9061 cggcgacgtg catcccgtgg taggcgtgct ggcgggtgac caggagcgtg cgctcggtct
     9121 cgccgcggag ctgccagtac cggcgggcca tcttcgtcgc cgtctcgatc gagtccgacc
     9181 cgccgctcgt caggaagacc ttcgagtcgg ggaccggcgc gagccggccg acgcgctcgg
     9241 cgagctcgat cgcgggccgg ttcgactggt cgccgaaggt cgagtacgcc gacaggcgca
     9301 tcatctgccg gtgcgccgcg tcggcgatct cctcgcggcc gtacccgacg ttgcagtacc
     9361 acagcgccgc ggtcgcatcg aggtagcgac gtccgtgctc gtcccagacg tgcgcgtcct
     9421 cgccgcggtc gatgacgagc tcgccggcgg actcgacggc ggccatgtcg gcgaaggagt
     9481 gccagaaggt acccacgggc tcgctcacgg tagcagtcgt atcggggccg atcgggagcg
     9541 ccttgaatgt tcaggagggt tcgtaggcga ggttggggcg cagccaggtc tccgcctcgc
     9601 gccggtccat cccgcaccgg gccgcgtact cctcgacctg atcgcgaccc agccgcccca
     9661 cgttgaagta ccgcgccgcc gggtgcgcga agtacaggcc cgagacgctt gccgcggggg
     9721 tcatcgcgaa gtgctcggtc aggtcgatgc cggcgctcgg ggcatccagc agctcgaaca
     9781 gcgtcgcctt gcgcgtgtga tcggggcagg ccggatagcc gaacgcgggc cggatcccgc
     9841 ggtagcgctc ggcgagcagg tcgtcgccgc tcatctccgg gcccgactcg taccactcgc
     9901 gccgggcgcg ctcgtgcaac cactcggcga acgcctccgc caggcggtcg gcgagcgcct
     9961 tgaccatgat cgcctggtag tcgtcgaggt cggtctcgaa ctgtgccgcg agctcgtcgg
    10021 ccccgaggcc ggcggtcacg gcgaacgccc cgatgtggtc cacgagtccc gtctcggcgg
    10081 gcgcgacgta gtcggcgagc gatctgctgg gccggtcgtc gccgtggtcg gcctgctgcc
    10141 ggagcatcgg gaaccgcggg ccgcccgaga gcaccacgtc gtccccctcg gccgacgcag
    10201 gccagaaccc gtacacgcct cgcgccgcga gagaacgctc ggcgacgatc ctgtcgagga
    10261 gctcgttcgc ggcctcgaac agctctcggg cggcctcgcc gtgccgcggg tggtccagga
    10321 tctgcggaaa gcggcccttg agctcccacg cgccgaagaa gaacgtccag tcgatgtagg
    10381 gcctgagctc ggccagatcg ggctcgacca cgcgggcgcc ggtgaacgcc ggctcgggca
    10441 ggtcctgctg acgccagacg atcggcgttc ggcgctcggt cgcgatccgg tacggcagca
    10501 gcggctgggc ctcgcgctcc gcgtggagca accggagtcg ctcctggtcc tcgcgattga
    10561 cgtggtcgag cttcgaacgc cgatcggggt cgaggagatc cgagacgacg cccaccacgc
    10621 gggacgcgtc gacgacgtgg acgacgggct ggccgtactc gggcgcgatc cgaacggcgg
    10681 tgtgctgccg ggaggtggtg gcgccgccga tcagcagcgg cagctcgagt ccgcgccgct
    10741 ccatctcctt ggcgacgccc tccatctcgc cgagcgacgg ggtgatgagc cccgagcagc
    10801 cgacgatgtc gcagccctcg gcggcggcgg tatcgagcag gacgtcgggc gggaccatca
    10861 cgccgagatc gatcacctcg tagttgttgc atcccagcac gacgcccacg atgttcttgc
    10921 cgatgtcgtg cacgtcgccc ttgaccgtgc acaggatcac cttgccttgg gcgcggaccc
    10981 cttcggggcc ggccaggagc ttctcctgct ccatgaaggg ctcgaggtaa gcgacggcgc
    11041 gcttcatcgc ccgcgcgctc ttgacgacct gcggcaggaa catcttgccc gcgccgaaca
    11101 ggtcgccgac gacctgcatc ccggccatga gcggcccctc gatcacgtcg agcggccgtt
    11161 cgtgggcctg cctcgcttcc tccgtgtcct gctcgatgaa ctcgtcgatc ccgtgcacga
    11221 gcgcgtgctt gaggcgctcc tcgacggtcg tctcgcgcca cgagaggtcg agctccttct
    11281 tcatcgcgcc gcccctgacg gtctcggcga acgagaccat gcgctcggtc gcgtcgggcc
    11341 gccggtcgaa gatgatgtcc tcgacgtgct cgagcaggtc ggcggggatg tcctcgtaga
    11401 gcgcgatctg gcccgcgttc acgatcgcca tgtcgaggcc cgccgcgatc gcgtggtaca
    11461 ggaaggcgga gtggatcgcc tcgcggacgg cgtcgttgcc gcggaagctg aaggaaagat
    11521 tcgagacgcc gcccgagacc ttgacccccg gcagccgttc cttgagccga cgggtggcct
    11581 cgatgaacgc cacagcgtag gcgttgtgct cctcgatccc ggtcgcgatc gcgaggatgc
    11641 aggggtcgac gacgatgtcc tcgggcacgt acccggcccg atcgatcagc agccccaccg
    11701 aacgctcgag gatctggagc ttgcgttcga cggtgtcggc ctgtccctgc tcgtcgaacg
    11761 ccatcacgac cacggcggcc ccgtagcgct tgacgcggcg cgccttctcg aggaactcct
    11821 cctcgccctc cttcaggctg atcgagttca cgatgccctt gccctggagg cacttcaggc
    11881 cggcttcgag cacgtcccac ttcgaggagt cgaccatcac cgggacgcgc gcgatctcgg
    11941 gctcggtcgc gatcaggttg aggaacgtcg tcatggcctt cacgccgtcg agcaggtcgg
    12001 cgtccatgtt cacgtcgagc atgttcgcgc cgctgcggac ctggtctgcg gcgatctcga
    12061 ccgcggcctg gtagtcgccg acctcgatca gccggcggaa tcgagccgag ccggtgacgt
    12121 tctgccgctc gccgatcatc acgaaccccg tctccggcgt gatcgagaag ggttccagcc
    12181 cgctgaacga ggtggcggtg cgctgcgccg ggaccctgcg cggcgagagg ccgtccacgg
    12241 cggcggcgat cgccctgacg tgctcgtcgg tggtcccgca gcacccgccg aggacgttcg
    12301 cgaggccgct ctcggcgaac tcgcgcaggt acttggaggt ggtctcgggc cgctcgtcgt
    12361 agccgccgaa cgcgttcggc aggccggcgt tcgggtggca ggtggtgtag acgggcgcga
    12421 tccgcgcgag ggtctcgagg tacgggcgca tctcggccgc cccgagcgcg cagttcacgc
    12481 cgacggtgag aggttccgcg tgggcgatcg agagccagaa cgcgtccacg gtctggcccg
    12541 agagcgtccg gccgctccgg tcggtgatcg tgaccgagat catgagcggc acgccgggtg
    12601 ccacgtcgct cgccgcagcg atcgccgcct tggcgttcaa cgtgtcgaag atcgtctcga
    12661 tcaaaagcag gtcgatgccg ccgtcgacga gcccacggat ctgctcggcg tacgcctcgg
    12721 ccatccggtc gaacgagacc gtccggtacg ccggctcgtt cacgcgagga gacagcgaga
    12781 gcgtctggtt cgtgggaccg acggacccgg cgacgaaccg atcggggccg aacgcttcgg
    12841 ccgcgcgacg ggcgatccgg gcgccggcca ggttgaggtc gtagaccgcc tcctcgaggc
    12901 cgtagtccgc ctgggcgatc ggcgtggcgg tgaacgtgtt ggtcgtgacg atgtccgctc
    12961 cggccgcgag gtaggagcga tggatgtcct ccacgacgtc ggggcgcgtg aggttcagga
    13021 ggtcgggatc gccggcgacg tcgcgcgggt gatcccggaa gttctcgcca cggtagtcgc
    13081 cgtcggcgag ggaccgcgcc tggatcgcgg tcccgtaggc gccgtcgagc acgaggatcc
    13141 gttcggccag cagggcctcg agtcgttccc gcatgggtca acgctaacct cggtgccccg
    13201 gtgtgaccag cacgcgaggt cctcgttccg tcgaggccac cacgtggggt acgctgccag
    13261 gtccgggttc gtcgagccgg tggaggaagc ggcgttgggc aaggacgcgt tcgaaccgct
    13321 cacgcgcgcc gttcccggcg cgatcgcgac cggcgggatc ggcgattctg ctccctcggg
    13381 gcgacgccga ccggaccggc cgctcgggag gcggggagcc gacctgctcg cccaggtccc
    13441 gttgttcgag gggctgtcgc gaaggcacct caagcagatc gccgagcgcg ccgacgagat
    13501 ctcgttccga cagggcgaga ccatcgtcga ggcggatcag ccgggcggct cgttcttcgt
    13561 gatcgtcgag ggagaggtgc gagtcgtgcg gggcgaccgc acgatcgcgc gcgccggacc
    13621 gggcgagttc ttcggcgaga tctcactgct cgacggcgga cccaggaccg cctcggtgat
    13681 cgccgagact cccgtcgtcg cgatccggct cttcaaggcc tcgttcgaca agatcgtccg
    13741 ggaggagccc cacgtggccg gcaagatcct ggttgtagtg gcccgccggc tccgcgaggc
    13801 cgagaagacc gtcaccgcgt gatgctggcg ttcaccgcgt acacctggtg gaagttcttg
    13861 cacgtcgccg gcgtgatcgc gttcgtgatg ttccacggcg tgtcgatgac ggttgcgctc
    13921 cagcttcgca gggagcggga ccgagggcgt atcgccacca tcacgcaact gtcgggctcg
    13981 tccctcaggg gcatgtacgt agcgctgctg tggctgatcg tgttcggcgt gatcgccggc
    14041 atccaggccg actggtggaa cgacggctgg ttctggatct cggtcggact gctggtcgtg
    14101 gcgatcgccg agatgtcggc cgtcgcccgg ccgtactacg agcgggtgaa ggaggcgatc
    14161 gagattcggc cgtccggcgt gccgcgccgc agcgacgagg agctcgacga gatcctccgg
    14221 tcgccgatcg gcctgtggaa cacggtgttc ggcgtcgcgg tgctcgcggt catcgcctgg
    14281 ctgatgatct tcaagccgtt cgtggtcctc tgacgacgcg ctacaggtcc gtcggcgcga
    14341 ggggccgctc cgcgggcccg gcaacgacct gaccggtggc cgagtaccgg gacccgtgac
    14401 aggggcagag ccagccgtgc tccgaggcat cccacttgac cgtgcagccc tggtgcgtgc
    14461 acgtggcgga caggatcgtg aggttcccct tcttgtcccg gtagacggcc accttgtggt
    14521 cggccatctt cacgatccct ccctcgtccg gcccgatctc gtcaaggttc tgtgccacgg
    14581 tccgcgcgac cagcacgtcg cacggcgcgg actcggcgac gtccctcggc accgagccca
    14641 gcatggcggc cttggccccg gtcatgccgc ggttgccgat caccaccagg tcgaacccct
    14701 cggtagccgc cgcgtcgacg atctcgtcgg aggggtcgcc cgagcggatc tcgatcgccg
    14761 aggtgacgcc gctctctgag ccgatcgtcg cgacggtgtc ctgcagcacg agctcgccgg
    14821 tcttcgggtg gccgacgaag agcagcgtga ccgatgcctt gagggccttc gccagcacgt
    14881 agcccttcct cgcggcgcgg tctgccgtgg aggagccgtc ggtggcgatc agcatccgcc
    14941 gatagggcgg tgccggtttc tcgtgttcgc cgatcgtgcg cgcccgcgtg agaagcacgt
    15001 cgcacggcgc gtgcgtcacg acccgccgcg acacgctgcc gaacagctga tcggacgtcg
    15061 agaacccgac gctcccgatc acgatgagct ccgcatcctt ctcgtccgcg gtcatgagga
    15121 tcgcgtcggc cggcgcttcg gcggaaagtg cgagctcgtg acgggccccc tcgcggtcgg
    15181 cgatctcctt cgcccgggcg acggcctcgt tcgcccggtc ggggttggcg taggcgtggg
    15241 cgatcgtgag cttggcccca ccgatcgacg ccagggtcga ggcgatcgtc tcggccgtct
    15301 ccgccgagtc tgatccgtcc gtcgccacca cgatccgtcg gtacaccatg ccgatgagcc
    15361 ccttcctatc cgggaggcgt ccgcgagagt accgcctcga gctcggagag ggtcgcctcg
    15421 tccagctcga cctcgcccgc cgcggcgttc gaacggacgt gatcggggtt gcggctcccc
    15481 gcgatcgccg cggtcacccc cggttgcgcc gcgttccacg cgagcgcgag ctgggcgagc
    15541 gtgatcccga gccgtctcgc gatcggtcgc aaagcatcca cggcggcgag attgcgctcc
    15601 cggttgcccc gggcgaacag cgagccccct tcatcctcgc ctgcgcggaa gtcgccctcg
    15661 tcgaaccgcg tctcggtcgt gatcgcgcct gtgaggagcc cgtaggcgag cggggcgtag
    15721 gacagcacgc ccgtgccctg ctcgccgcac caggcgatca ggtcgcggtc ctcgagcctg
    15781 agcatcgaga actcctgttg gagcgagtcg acatgccgga tcgcctcgca ccgttcgatc
    15841 agcgcgcggt cgaagttcga cacgccgatc gatcggacga gcccgtcgtc caacagcgcg
    15901 gccatcgcgt tccaggtctc ctcgaggggg acgccgcgtt cgtcgggcca gtggagctgg
    15961 tacaggtcga cgtgatcagt tccgagccgc cgcagactcc cctcgcaggc cgaacgcacg
    16021 tgggcggccc ggaagcccga accttcaggc tgcggggcga cctttgtggc gatcaggacc
    16081 tcgtcccgcc gaccggcgat ggcccgagcc acgagctcct ccgacttgcc cccgccgtag
    16141 acctcggccg tgtcgatcct gtcgatcccg gtctcgaaca ccgtccggat cgcccggagc
    16201 acctgctcct cggggggcgg gtcaccccat tcggatccgc cccccgcctc ccaggccccg
    16261 aacccgatcg cggacagttc gggacccctc gaacccagcc ggttccttcg catgtgctcg
    16321 tcctccgtgc gtgctcgggc cgtcggcggg gcgtcgcacg cgtccgcctc aatctaccgg
    16381 gtggacctgt ccggtccgcg ggtgcgcggt acgctaggtg tgcaagcgat cctcaagatc
    16441 cgatccggag gaggcggccg ggccacgccc ggcgacgatg accaagcagg tggaggagaa
    16501 gcagcgcgtc gtgatccggt tcgccggcga ttccggtgac gggatgcagc tgacggggga
    16561 ccggttcacc aatgccacgg cggtcctggg caacgacctc gcgacgatgc cggacttccc
    16621 ggccgagatc cgggcccctg ccggcaccgt gcacggcgtc tcggcgttcc agatccagtt
    16681 cgcctcgacc gacatcttga ccccgggcga ccaccccaac gtcctggtcg cgatgaaccc
    16741 ggcggccctg aaggccgagc tcggctcggt cgagaagggc ggcacgatca tcgtgaacga
    16801 ggacgccttc accccgcgga acctgcagaa ggccgggtac gcctccgatc cgctcaccga
    16861 cggcctcctc gacgcctacc agatctaccg ggtcccgatg acctcgatca ccgttcgtgc
    16921 cgtcgaggag ctcgggctca cgcgcaagga ggccgagcgc tccaagaaca tgttcgcgct
    16981 ggggctcgtt tcatggatgt acgggcgccc gaccgacgtc accctcaact ggctgcacaa
    17041 gaagttcggc gacaagccgc agctgtacga cgccaacgcg gccgccttca aggcgggcta
    17101 taactttggc gagaccacgg agctgttcgc gacctcgtac gaggtcaagc cggccccggc
    17161 cgagccgggc gtctaccgca acgtgtcggg cgcgcaggcc ctcgcgtggg ggctcgtcgc
    17221 ggcggccgag cggagcggtc tgcagctgtt ctacgcgagc tacccgatca cgcccgcctc
    17281 cgagctgctc cacgagctct cgcggctcaa gaacttcagc gtgatgaccc tgcaggccga
    17341 ggacgagatc gcggccgcca acgtggctct cggcgcggcc ttcgccgggg acctcggtgt
    17401 gaccgggacg agcggacccg gaatggacct gaaggccgag accctcgggc tggccgtgat
    17461 gctcgagctg ccgatggtga tcgtcgacgt ccagcgcgcg gggccgtcga ccgggatgcc
    17521 gaccaagacc gagcaggcgg acctcctgct cgcgatgttc ggccgccacg gggagtcgcc
    17581 gatgccgatc gtcgcggcgc gttcctcgtc gcactgcttc gatgcggcga tcgaggccgc
    17641 ccggatcgcg atcacgtacc ggacgcccgt catcctgttg tcggacacgt tcctcgcgaa
    17701 ctcgtccgag ccgtggaggc tcccggacgt cgagagcctc ccgacgatcg acccggcgtt
    17761 cgcgaccggt ccgaacgccg gacaggagtt cctgccctac ctgcgcgacg agaagctcgc
    17821 ccgtccgtgg gcgctcccgg ggacgccggg cctccgacat cggatcgggg ggctcgagaa
    17881 agaagacggg accggtgaca tcagctacga gcccgagaac cacgagcgca tgacgcagct
    17941 gcgggctgcg aaggtcgcga agatcgccga cgacatcccg ccgctcgagg tcgacgatcc
    18001 acacggggac gcggagctcc tggtgctcgg ctggggatcg acctacggga cgatcctcgc
    18061 ggccgcgagg cgcgtgcgcg aacgcggcaa gaaggtggca accgcgcacc tggtccacct
    18121 gaacccgatg cccgccaaca ccggcgggat cgtgaagcgc tacgcgaagg tgctgatccc
    18181 cgagctcaac acggggcagc tcgcgatgct gatccgctcg gagcacctgg tcgacgcgaa
    18241 gaccttctcg aaggtgcagg gccagccgat cttcgcggag gagctggagg acgagatcct
    18301 gcgggtgatc ggggaggaga cgcgggtggg cggcgacaag gtgatggacc gatgagcgac
    18361 agtggcaacg gaacgacgaa cggcgatcgc gcgatcggca acggcgacgg cgcggtcggg
    18421 acactgacga agaaggcctt caccagcgac caggaggtgc gctggtgccc gggctgcggg
    18481 gactacgcga tcctgtcgac ggtccagggg ttcatgcccg agctcgggat cgaaccggat
    18541 cggacggtgt tcgtctcggg gatcggctgc tcgggtcgat tcacgtacta catggacacc
    18601 tacggcgtgc acggcatcca cggtcgcgcc cccgcactcg ccaccgggat cgccacggcg
    18661 aggccggacc tgtcggtgtg ggtgatcacg ggggacggcg acgggctgtc gatcggcggc
    18721 aaccacctga tccacgccct gcgccgcaac gtgaacctga agatcctcct gttcaacaac
    18781 cagatctacg ggctgaccaa gggccagtac tcgccgacct cggaggaggg caaggtcacg
    18841 aagtcgacgc cgttcgggtc ggt
//
LOCUS       DATBRK010000060         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1016453, whole genome shotgun sequence.
ACCESSION   DATBRK010000060 DATBRK010000000
VERSION     DATBRK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016453"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VIH70_01860"
     CDS             <1..541
                     /locus_tag="VIH70_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEY8115140.1"
                     /translation="EKLQERLARLAGGVAVIKVGAATEVELKEKKHRIEDAVQSTKAA
                     VEEGVVPGGGVALLNAQSVLDKVDLDGDELTGSLIVRRALEEPLKQIAANAGLEGGVV
                     IEKVRALDVGFGLNAATGEYEDMFKAGIIDPTKVTRSALQNAASIAALFLTTEAVVAE
                     KPEKEKAPPMPGGGGDMDF"
     gene            745..972
                     /locus_tag="VIH70_01865"
     CDS             745..972
                     /locus_tag="VIH70_01865"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEY8115141.1"
                     /translation="MGTARLHLTFPGTLASEPILHRLATEFGLVTNIRRANIEERGGW
                     VIVEVDGDDDRIAEAVAWLAERGLGVDRIDE"
     gene            1024..1422
                     /locus_tag="VIH70_01870"
     CDS             1024..1422
                     /locus_tag="VIH70_01870"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8115142.1"
                     /translation="MLEKRIEIRWRDVDAYGHVNNAVYLNYLEEARDAWVQAILGPVS
                     GTTWDFVLARVEIDFRAELTQDDHAVIVRCTLDRIGRSSVGTREEVRKQDGTISAEAR
                     SVVVARDPETGRSRPLTKPEREALEAERTD"
     gene            1463..1672
                     /locus_tag="VIH70_01875"
     CDS             1463..1672
                     /locus_tag="VIH70_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115143.1"
                     /translation="MLGEREAVVVEVKPWDVHGVGYVDVTVVYPDRMLETARLGPESV
                     PEDLEAGERVMVNKAVNMIIGIRRA"
     gene            1672..1866
                     /locus_tag="VIH70_01880"
     CDS             1672..1866
                     /locus_tag="VIH70_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115144.1"
                     /translation="MFVWAYLDGAGEEIGRSPRFAAADEAEDWIGSSWPELLENGVLA
                     VVLVDQEGGRQLYRMGLGAE"
     gene            complement(1873..2376)
                     /locus_tag="VIH70_01885"
     CDS             complement(1873..2376)
                     /locus_tag="VIH70_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115145.1"
                     /translation="MDELSGWFAGRIPADWFAGTPEVVFDRDEILVVGTLSDPELPSD
                     ATVEVAASARVARIDGFREDTREHRMRIAQEAQHRFGRRVSWGASCGDVRKLFTTVSV
                     PAMTRLRLDERRVLDTLVEGGVARSRSHALAWCVRLVAQKQDEWLKDLREALSDVEKV
                     RGEGPTS"
     gene            2550..3161
                     /locus_tag="VIH70_01890"
     CDS             2550..3161
                     /locus_tag="VIH70_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115146.1"
                     /translation="MNLTENVTRKPVAGILGIVGGALMAVGSFLAWAEVSGGGTSVTA
                     KGVDGSDGYITLGAGLVAVVAGIVMTMEARRVLAVLATLAGIVGGGLGLYDALTAKDS
                     VLDAAAEELAPAFGVSAQAVRTALDQAIDAGQLSVSISIGLYIVIAGGVVALAGGILG
                     LRSGEPAVPGQTAALATPATTIGSPDATPAPMPPSTSQPDPGA"
     gene            3162..>3625
                     /locus_tag="VIH70_01895"
     CDS             3162..>3625
                     /locus_tag="VIH70_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115147.1"
                     /translation="MPGRAVLAGALALAGGVLAVVGSFLSWAEVSAGPFTEQARGIDG
                     WEGKAAIVSGVVMGAAGIRVLAGSHQAMARLRPSAAIGGLVAVGVGLYTALTVRDQLV
                     EAVATKLPRAEVERALGSGLLELTIGLGLYLVIAGGLQGVVAALVALGFRDEP"
BASE COUNT          521 a         1231 c         1347 g          526 t
ORIGIN      
        1 agagaagctg caggagcgcc tggcgcgcct cgccgggggc gtcgcggtca tcaaggtcgg
       61 cgccgcgacc gaggtcgagc tcaaggagaa gaagcaccgt atcgaggacg ccgtgcagtc
      121 cacgaaggcc gccgtcgaag agggggtcgt gccgggcggc ggcgtcgccc tgctgaacgc
      181 gcagtcggtg ctggacaagg tggatctcga cggtgacgag ctcaccggct cgctcatcgt
      241 ccggcgggcg ctcgaagagc cgctcaagca gatcgccgcc aacgccggtc tcgagggcgg
      301 cgtcgtgatc gagaaggtcc gtgccctcga cgtcgggttc ggtctgaacg ccgcgacggg
      361 cgagtacgag gacatgttca aggccggcat catcgacccg accaaggtga cgcgctccgc
      421 gctgcagaac gcggcgtcga tcgcggccct gttcctcacc accgaggcgg tcgtggccga
      481 gaagcccgag aaggagaagg ccccgcccat gcccggaggc ggcggcgaca tggacttcta
      541 ggtccggtcg caagcacatc gaagcggccc tcctcctcgg ggggccgctt cgctgccccg
      601 gggtgccggg gacgggtgaa ccggacggcc ggtcgaggcg aacgtccggc aggagccccc
      661 tctctcacgc cgaggccccg gagccatccg gggcctcggc gtatcccggg tcctccgcca
      721 ccgcccgccg tgccagaatc ggccgtgggc acggcgcggc tccacctcac gttccccgga
      781 accctcgcgt ccgaaccgat cctgcacagg ctggcgaccg agttcggcct ggtgaccaac
      841 atccggcgcg cgaacatcga ggaacgaggg ggctgggtga tcgttgaggt cgacggggac
      901 gacgaccgga tcgcggaggc cgtcgcgtgg ctcgccgagc ggggactcgg ggtggaccgg
      961 atcgatgagt gacccgcatg ggctcccgcg gtccgcgtcg cgtccggaac ggtcccggcc
     1021 ctgatgctgg agaagcgcat cgagatccgg tggcgtgacg tcgacgcgta cggccatgtg
     1081 aacaacgcgg tctacctgaa ctacctcgag gaggcgcggg acgcgtgggt gcaggcgatc
     1141 ctcgggccgg tgtcgggcac cacctgggac ttcgtccttg cccgcgtgga gatcgacttc
     1201 cgcgccgagc tcacgcagga cgaccacgcg gtgatcgtcc ggtgcaccct ggaccggatc
     1261 gggcgctcga gcgtcgggac gcgcgaggag gtgcgcaagc aggacgggac gatctcggcg
     1321 gaggcgcgtt cggtcgtggt ggcccgagac ccggagaccg ggcgttcgcg accactcacc
     1381 aagccggaac gcgaggccct ggaggccgaa cggacggact gacgcccccc gcgcctcacg
     1441 ggttccgtta gcctgccgga ccatgctggg cgagcgtgag gccgtggtcg tcgaggtgaa
     1501 gccgtgggac gtccacggcg tcggctacgt ggacgtgacc gtcgtctacc cggatcggat
     1561 gctcgagacg gctcgcctgg ggcccgagag cgttcccgag gatctcgagg cgggggagcg
     1621 cgtgatggtg aacaaggcgg tcaacatgat catcgggatc cgcagggcct gatgttcgtc
     1681 tgggcctacc tcgacggcgc cggcgaggag atcgggcgct cgcctcggtt cgccgccgcc
     1741 gacgaggcag aggattggat cggctcgtcc tggcccgagc tgctggagaa cggtgtcctg
     1801 gccgtcgtcc tggtcgacca ggagggcggg aggcagctgt atcggatggg tctcggcgcc
     1861 gagtaacgcg cgctaggagg tggggccctc gccccgcacc ttctccacgt ccgagagcgc
     1921 ctcgcgcagg tccttcagcc actcgtcctg cttctgcgcg accagccgca cgcaccacgc
     1981 gagcgcgtgg ctgcgcgaac gcgcgacgcc cccctcgacg agcgtatcga ggacccggcg
     2041 ctcgtccagt cggagccgcg tcatcgccgg cacgctcacc gtggtgaaca gcttgcggac
     2101 gtcgccgcag gacgcgcccc aggagacacg ccggccgaac cgatgctgtg cctcctgcgc
     2161 gatccgcatc cggtgttcgc gcgtgtcctc gcggaagccg tcgatccgcg cgacgcgcgc
     2221 cgaggccgcg acctcgacgg tggcatccga cgggagctcg gggtccgaga gcgtcccgac
     2281 caccaggatc tcgtcgcggt cgaagacgac ctccggggtt ccggcgaacc agtcggccgg
     2341 gatcctgccg gcgaaccagc cgctcagttc gtccatcgaa gccgtgccgt gtgtgttcat
     2401 acccggagat tacaccatta cacgtaatcg tcaaggtccc aacggccctc ggtcccggtt
     2461 cggcggcccc ctcggggatg cgctcgactc cgccgtgccc agatggaagg atggccggcg
     2521 accggcgctc gcccgggatc ggaggcccaa tgaacctcac ggagaacgtc acgcgtaaac
     2581 cggtcgccgg gatcctcggg atcgtcggag gtgccctgat ggccgtggga tcgttcctcg
     2641 cctgggcgga ggtgtccgga ggcgggacga gcgtgaccgc gaagggcgtc gacggctcgg
     2701 acgggtatat cacgctggga gcgggcctcg tcgcggtcgt ggcgggcatc gtgatgacga
     2761 tggaagccag gcgggtgctc gccgtgctgg cgaccctcgc ggggatcgtg ggcggtggcc
     2821 tcggtctgta cgatgcgctc acggcgaagg acagcgtgct cgatgccgcg gccgaagagc
     2881 tcgcgccggc gttcggggtc tccgcccagg cggttcggac ggccctggac caggcgatcg
     2941 acgccggcca gctcagcgtt tcgatcagca tcggcctcta tatcgtgatc gccggcggtg
     3001 tcgtggccct cgccggcggg atcctcggcc tgcgttcggg cgagcccgcc gttccgggcc
     3061 agacggccgc actcgcgaca ccggcgacca cgatcggtag tccggacgcg acgcctgctc
     3121 cgatgccccc gtcgacctcg caacccgacc ccggcgccta gatgccgggt cgagccgttc
     3181 tcgcgggggc actggcgctc gcgggcggcg tactggcggt cgtcggttcc ttcctctcct
     3241 gggccgaggt ttccgcggga ccgttcaccg agcaggcgag gggcatcgac ggctgggagg
     3301 gcaaggcggc gatcgtctcc ggtgtggtga tgggcgccgc cgggatccgt gtcctggcgg
     3361 gatcgcacca ggcgatggct cgtctgcggc cgagcgccgc gatcggcggt ctcgtggcgg
     3421 tcggcgtcgg tctctacacg gccctgacgg tccgggacca gctcgtcgag gccgttgcga
     3481 ccaagctccc cagggccgag gtcgaacgcg cgctgggcag cggcctgctc gagctcacga
     3541 tcgggctcgg cctctacctg gtcatcgccg ggggcctcca gggcgtcgtc gcggcgctgg
     3601 tcgcgctcgg cttccgggac gagcc
//
LOCUS       DATBRK010000061         5689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2750461, whole genome shotgun sequence.
ACCESSION   DATBRK010000061 DATBRK010000000
VERSION     DATBRK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2750461"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VIH70_01900"
     CDS             <1..772
                     /locus_tag="VIH70_01900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8115148.1"
                     /translation="WLRHAELETNPTRRPLVRLEARRVRKYEAGLLARFDVVFAVSER
                     DRAALAALAPAGPEIALLPNVADPALLGRPELTPPPEPVLLFLATLSWPPNADGLSVF
                     LGNDLPALLERVPDARLVVAGSGAPERLVRQVHQSRGAEFAGPVDDDEVLYTRARCFV
                     DVGVGGSGTKVKVLNALARGLPVVTRPDGAAGLEVSSGEHLLVGEDREGMVASLEFVI
                     QEDAAWTTLSRNGRDLVRRRYVPEVAFGALDDALARRS"
     gene            complement(801..2231)
                     /locus_tag="VIH70_01905"
     CDS             complement(801..2231)
                     /locus_tag="VIH70_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY8115149.1"
                     /translation="MKSPTPPTDDARGPKPSGAHPHVRRVFLAATATVSAFVVVVAAI
                     VMGGYFWASASIPTIPEPPPSASGSTGPQDIAGTCDERACNYLLLGSDSRKGLTPEEL
                     IAFGTDAHIGGENRSDTIILVHTRPDRREVVFLSFPRDLWVDIPGVGEGKINSAFEGG
                     VNGGGPQRVARTVRSISGMQIHHVLYVNLLGFQRVVDALGGVDMCVPYPMEDPLTLLD
                     IRAGCQHFDGRTALAYVRTRHQPCDTVPDFARISRQQQFLRAVIAKVLRPGELLKLQD
                     LIPQLLGNLVVDQGLNPAELVYLAGLLKDVGTDNADFRALPTVPEGIYDSNGTFVSIV
                     RAVQPDADELLRRIREGRPLGDLGQELPQTPPSPANIVVSVVDRDGSPVATDVFDILT
                     EGGFNTSPGVVGLTEIRPPTKGAMILYRPDAEDEAKVVGTYFGNLELVPAPPGTLPKG
                     QDAAVVVGGRYEIPPPPTDEPVECPT"
     gene            2384..4255
                     /locus_tag="VIH70_01910"
     CDS             2384..4255
                     /locus_tag="VIH70_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8115150.1"
                     /translation="MRTHGITTEEELIERSTSDVEWFWDAAIRDLGLEFFEPYGSILD
                     TSRGVEWATWFGGGSVSLAHNCVDRWAERTPGATAIAWEGEEGTTRRVSYRELREASD
                     RLANGLAALGVRPLDAVGIFLPPTPEAVVAVMACSKIGAVWLPLFSGFGADAIAKRLE
                     DAEAAVLITADGSPRRGRVVPMKETADEAVDAGGSVRHVIVVARLGRTDAPWRPERDL
                     RWDELLASNADRFETAHLDPEHPLFIAYTSGTTGKPKGAVHVHGGFLVKIAEEVAYQT
                     DVQPGDLLFWFADLGWIMGPWEIVGATALGATVFLYDGAPDHPGPDRLWSMVERHRIT
                     HVGVSPTLVRALIRSGEEPVRSHDRSSLRILASTGEPWNPEPYRWFSEVVGEGRLPII
                     NLSGGTEVGACFLSPHPISPLEPCSLRGPALGMDVAVFRPDGTVADPGEVGELVCRKP
                     WPSMTRGLRGDPERFLDAYWRRFPGVWCHGDWASVDQHGQWFLHGRSDDTMNVAGKRL
                     GPAEVESILTLHPAVTESAVVGVPDEVKGEAIWCFVVANDEVSDGLADELSALVARHL
                     GKAFRPARVLVVPELPRTRSAKILRRAIRATVVGEDPGDLSSLENPSSLASIRAALG"
     gene            4311..5156
                     /locus_tag="VIH70_01915"
     CDS             4311..5156
                     /locus_tag="VIH70_01915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8115151.1"
                     /translation="MSAARDRTLSVLAVGVDPGALEGCTLERAEDLLGALANLADGGF
                     DVVVTSLDLPDATGTDVIGSLRERAPGVPVIVVGDALAEPMDAFAAGASDVVPEDAAA
                     DLVARAIRYAATLREVEAGLRRFQAFDELTGLLNLFGFDLLANHHFRLADRSKRPVLI
                     VFLRVDERGETPGEAAVADAARVISSAVRVSDVVARVDSGAFCVLLPGAEPGSEPIVL
                     ERIIEAVASHNAGTGHETGPSLSVGAASYDPEHPVPLGELIVAADQRMRRPSDAPGSP
                     RPRSR"
     gene            complement(5281..>5689)
                     /gene="acnA"
                     /locus_tag="VIH70_01920"
     CDS             complement(5281..>5689)
                     /gene="acnA"
                     /locus_tag="VIH70_01920"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075957.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY8115152.1"
                     /translation="EGVPLVVLAGKEYGSGSSRDWAAKGPALLGVRAVIAESFERIHR
                     SNLVGLGVLPLQYVAGDSAATLGLDGTETFDVSGIAGGGIEPGRRVRVTARRPGGEDT
                     AFEAVLRVDGAAEVDYVRHGGILQMVLREMLAG"
BASE COUNT          757 a         2046 c         2060 g          826 t
ORIGIN      
        1 gtggctccgt cacgccgagc tcgagaccaa tccgactcgc aggcccctgg tccggctgga
       61 ggcccggcgg gtccggaagt acgaggcggg tctgctcgcg cggttcgacg tggtgttcgc
      121 ggtctccgaa cgcgaccggg cggccctcgc cgccctcgct cccgccggcc ccgagatcgc
      181 cctgctcccc aacgtcgccg accccgcctt gctcgggcgc cccgagctca cgccgcctcc
      241 cgaacccgtg ctgctgttcc tcgccacgct ctcgtggccg cccaacgccg acgggctctc
      301 ggtgttcctc ggcaacgacc tcccggcgct gctcgagcgc gtgccggacg cccggctcgt
      361 cgtggcagga tcgggggcgc ccgaacgcct cgttcgccag gtccaccaga gccgcggcgc
      421 cgagttcgcc ggcccggtcg acgacgacga ggtcctctac acgcgggcgc ggtgcttcgt
      481 cgacgtcggc gtcggcggat ccggcacgaa ggtgaaggtg ctgaacgcac tcgcgcgggg
      541 cctgccggtc gtgacgcgcc ccgacggcgc cgccgggctc gaggtgtcct cgggcgaaca
      601 cctgctcgtc ggcgaggacc gcgaggggat ggtcgcctcg ctcgaattcg tgatccagga
      661 ggacgccgcc tggacgaccc tgagccgcaa cggccgggac ctcgtccgcc gccggtacgt
      721 gcccgaggtt gcgttcggcg ccctggacga cgcgctggcc cggcgttcct agatcgacct
      781 gcagtgcgga cgtcccgcag tcaggtgggg cactcgacgg gctcgtcggt gggcgggggc
      841 gggatctcgt agcgcccgcc gaccacgacc gccgcgtcct gccccttcgg cagcgttccc
      901 ggcggcgccg ggacgagctc caggttgccg aagtaggtcc cgacgacctt ggcctcgtcc
      961 tcggcgtcgg ggcgatacag gatcatcgcg cccttggtcg gcggccggat ctcggtgagg
     1021 ccgacgacgc ccggcgatgt gttgaacccc ccctcggtca agatgtcgaa gacgtccgtc
     1081 gcgaccgggc tcccgtcgcg gtcgacgacc gagaccacga tgttggccgg cgaaggcggc
     1141 gtctgcggca gctcctggcc gaggtcgccg aggggcctgc cctcgcggat ccggcggagc
     1201 agctcgtccg cgtcgggctg gaccgcgcgg acgatcgaga cgaacgtgcc gttcgagtcg
     1261 tagatcccct cgggcaccgt gggcagcgcg cggaagtccg cgttgtcggt cccgacgtcc
     1321 ttgaggagcc ccgccaggta gacgagctcg gccgggttga gcccctggtc cacgacgagg
     1381 ttcccgagca gctgggggat gaggtcctga agcttcagga gctcgcccgg ccggagcacc
     1441 ttggcgatca ccgcgcgcag gaactgctgc tgccggctga tccgcgcgaa gtccggcacg
     1501 gtgtcacacg gctggtgccg tgtccggacg tacgcgagcg ccgtccgccc gtcgaagtgc
     1561 tggcagccgg cccggatgtc gagaagcgtc agcgggtcct ccatcgggta cggcacgcac
     1621 atgtcgacgc cccccaacgc atcgacgacc cgctggaacc cgagcaggtt gacgtacagg
     1681 acgtgatgga tctgcatccc gctgatgctc cggaccgtcc gggctacgcg ctggggaccg
     1741 ccaccgttca cgccgccctc gaacgcggag ttgatcttcc cctcccccac ccccgggatg
     1801 tcgacccaca ggtcgcgcgg gaacgagagg aaaacgacct ccctccggtc gggccgcgtg
     1861 tggaccagga tgatcgtgtc cgaacggttc tcgcccccga tgtgcgcgtc ggtgccgaac
     1921 gcgatcagct cctccggcgt cagccccttg cgggagtcgc tgccgagcag caggtagttg
     1981 cacgcgcgct cgtcgcacgt gccggcgatg tcctgcggac cggtggaccc ggaggccgaa
     2041 ggcgggggct cggggatcgt cgggatcgac gcgctcgccc agaagtagcc gcccatgacg
     2101 atcgcggcga ccaccaccac gaacgcggag acagtcgcgg tcgccgccag gaacacccgc
     2161 cggacgtgcg gatgcgcgcc ggacggcttc gggccgcggg cgtcgtcggt cggtggcgtg
     2221 gggctcttca tgtcgtcggg ggtcataggc tgggttcggg aaccgcgccg ttcccgcgca
     2281 gcagagcgta cccgtaggcc cggagaagga agcggggcag gccgtgacgt tcgtgtggag
     2341 accctcgccc gaggtcgtgg agcgagcgaa tatcacgcgg ctcatgcgca cccacggcat
     2401 cacgaccgag gaggagctga tcgagcgctc gacctcggac gtcgagtggt tctgggacgc
     2461 cgcgatccgc gacctcggcc tcgagttctt cgagccctac ggatcgatcc tggatacctc
     2521 gcgcggggtc gagtgggcga cgtggttcgg cggcggctcg gtgagcctcg cgcacaactg
     2581 tgtcgaccgg tgggccgaac gcacccccgg cgccaccgcc atcgcgtggg agggcgagga
     2641 gggaaccacg cggcgcgtct cgtatcggga gctccgagag gcctcggacc ggctcgcgaa
     2701 cggtcttgcc gcgctcggcg tgcggccgct cgacgcggtc gggatcttcc tgccgccgac
     2761 gccggaggcg gtggtagcgg tgatggcgtg ttcgaagatc ggcgcggtgt ggctgcccct
     2821 gttctcgggg ttcggcgccg atgcgatcgc gaagcggctc gaggacgcgg aggccgccgt
     2881 gctgatcacg gccgacggct ccccgcggcg cggcagggtc gtaccgatga aggagaccgc
     2941 ggacgaggcg gtggatgcgg gcggatcggt ccgccacgtg atcgtcgtgg ctcgcctggg
     3001 ccggaccgac gcgccgtggc gccccgagcg cgatctgcgg tgggacgagc tcctcgcctc
     3061 gaacgcggac cggttcgaga ccgcccacct ggatcccgag cacccgctgt tcatcgcgta
     3121 cacgagcggg acgacgggca agccgaaggg cgcggttcac gtccacgggg ggttcctggt
     3181 caagatcgcc gaggaggtcg cctaccagac cgacgtgcag ccgggtgatc tcctgttctg
     3241 gttcgccgac ctcggctgga tcatggggcc gtgggagatc gtcggcgcca ccgcgctcgg
     3301 cgcgacggtg ttcctctacg acggcgcccc cgaccacccg ggcccggacc gactctggtc
     3361 gatggtcgag cggcaccgca tcacgcacgt cggggtctcg cccacgctcg ttcgcgccct
     3421 gatccggtcc ggcgaggaac cggtccgatc ccacgaccgg tcctcgctgc ggatcctcgc
     3481 gtcgaccggc gaaccctgga accccgagcc ctaccggtgg ttctccgagg tggtcggcga
     3541 gggccggctg ccgatcatca acctgtcggg cgggaccgag gtcggggcct gcttcctgtc
     3601 gccccacccg atcagcccgc tcgagccctg ctccctgcgc ggcccggccc tcgggatgga
     3661 cgtcgcggtg ttccgaccgg atgggacggt ggccgacccg ggcgaggtcg gcgagctcgt
     3721 ctgccggaag ccgtggccga gcatgacccg gggcctccgc ggtgaccccg aacgcttcct
     3781 ggacgcgtac tggcgccgat tcccgggggt ctggtgtcac ggcgactggg cgagcgtcga
     3841 ccagcacggc cagtggttcc tccacgggcg gagcgacgac accatgaacg tcgcgggcaa
     3901 gcgcctcggc cccgccgagg tggagagcat cctgacgctg cacccggccg tgaccgaatc
     3961 cgccgtggtc ggcgtcccgg acgaggtcaa gggcgaggcg atctggtgtt tcgtggtcgc
     4021 gaacgacgag gtttcggacg gtctcgcgga cgagctctcg gcgctcgtgg cccggcatct
     4081 gggcaaggcg ttccgcccgg cgcgcgtgct cgtggtcccc gagctcccac ggacgcgctc
     4141 ggcgaagatc ctccggcggg cgatccgcgc gacggtcgtg ggcgaggacc cgggcgacct
     4201 gtcgagcctg gagaacccct cgtcgctggc gtcgatccgc gccgccctgg gctaagtcga
     4261 cgcgaggatg gagcaacgac cgcccacgcg gtcgggatag gcttctggcg atgagcgcag
     4321 cgcgtgaccg gacgctgtcc gtcctcgccg tcggggtcga cccgggcgcg ctcgaaggct
     4381 gcacgctcga acgggccgag gacctcctcg gcgcgctcgc gaaccttgcc gacggcggct
     4441 tcgacgtggt ggtgacctcg ctcgacctgc ccgacgcgac gggtaccgac gtgatcggtt
     4501 ccctgcgaga gcgggcaccc ggggtcccgg tcatcgtcgt gggcgacgcc ctcgcggaac
     4561 cgatggacgc gttcgcggcc ggcgcaagcg acgtcgtgcc cgaggacgcc gcggccgacc
     4621 tggtcgcccg tgccatccgc tacgccgcca ccctgcgtga ggtcgaggcg gggctgcgcc
     4681 ggttccaggc gttcgacgag ctcaccgggc tgctgaacct ctttggattc gacctgctcg
     4741 cgaaccatca cttccggctg gccgaccggt ccaagcgtcc cgtgctgatc gtgttcctcc
     4801 gcgtggacga gcgcggcgag accccgggcg aggcggccgt cgccgacgcc gctagggtga
     4861 tcagctcggc cgtccgcgtc tccgatgtcg tggcgcgggt ggactcgggg gccttctgcg
     4921 tgctcctccc gggcgccgaa cccggttcgg agccgatcgt gttggagcgg atcatcgagg
     4981 ccgtcgcgtc ccacaacgcc ggcaccgggc acgagaccgg gccctccctg tcggtcgggg
     5041 cggcgtcgta cgacccggaa catccggtcc cgctcgggga gctgatcgtc gcggccgacc
     5101 agcgcatgcg acgtccctcg gacgcgccgg gctcccctcg gcctcggagt aggtgagaag
     5161 ttcccggccc gaccgggggc gaccatgagc acgagcgtgt tgcgaatgcc ccacgaggaa
     5221 cggacggcac gaaacggctc cctcttcgct cctcgaggcg ctcggtgccc gcgcgtcgct
     5281 tcagccggcg agcatctccc ggagcaccat ctgcaggatg ccgccgtgcc gtacgtagtc
     5341 cacctccgcg gccccgtcga cccgtaggac ggcctcgaac gcggtgtcct cgcccccggg
     5401 tcggcgggcg gtgacccgga cgcgccggcc cggctcgatc ccaccaccag cgatccccga
     5461 gacgtcgaag gtctcggttc cgtcgagccc cagcgtcgcg gccgagtccc ccgccacgta
     5521 ctgcagcggg agcacgccca acccgaccag gttgctccgg tggatccgtt cgaagctctc
     5581 cgcgatgacc gcgcggacgc cgaggagcgc gggacccttg gccgcccagt cgcgcgagga
     5641 accgctgccg tactccttgc ccgcgaggac gaccagcgga acgccctcc
//
LOCUS       DATBRK010000062         6979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_152160, whole genome shotgun sequence.
ACCESSION   DATBRK010000062 DATBRK010000000
VERSION     DATBRK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152160"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VIH70_01925"
     CDS             <1..307
                     /locus_tag="VIH70_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY8115153.1"
                     /translation="ALREAGATEVHTRITCPPIRWPCFYGIDMSTRQELIAADLTVEQ
                     IRDYVGADSLGYLSLGGMVAATGQDVGSFCRACFDGEYPIPIPEHAGKFLLEHPAKS"
     gene            304..1299
                     /gene="purM"
                     /locus_tag="VIH70_01930"
     CDS             304..1299
                     /gene="purM"
                     /locus_tag="VIH70_01930"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY8115154.1"
                     /translation="MSSEAYRRSGVDLDAAARAVELIRELGGGATRPELVEGVGGFAG
                     LFEIGEGRFLAAATDGVGTKLEIARRSGRLETVGIDLVAMCADDVVCTGAEPLFFLDY
                     LAVGSVDPERVAAIVAGVAEGCRRAGCALLGGETAEHPGVMAEDAFDLAGFCVGIVEE
                     ERRLGPDRVRDGDALIGLASSGLHANGFSLVRAALLVRAGYALERIVPPLRRPLVDEL
                     LEPTAIYAPLVLELAREGVLHAAAHVTGGGIVENLRRVLPEGLEADVAWRSWPEPPVF
                     ELLRAQTGVTEHDLRATFNLGVGMVLVVPPALAERAIAHAGGSGPAAFPIGVVRA"
     gene            1472..1855
                     /locus_tag="VIH70_01935"
     CDS             1472..1855
                     /locus_tag="VIH70_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8115155.1"
                     /translation="MGTKTTSPVLVVDDEPQIVWMLQFSLEAEGYQTLSAADGMTALD
                     EVREHHPKVVLLDIMMPVMDGWAFLEELQAIPAAERPRVIVVSARASLRDRAKAAELG
                     ADAFVAKPFSVDDLLVVLHDVERAV"
     gene            complement(1867..2667)
                     /locus_tag="VIH70_01940"
     CDS             complement(1867..2667)
                     /locus_tag="VIH70_01940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY8115156.1"
                     /translation="MIPAGSAELLDELRTLAVGLAHRAAEIQRGGLLGGFEIDRKSAA
                     GDLVTEIDRAAEHALVEGILDARPDDAILGEEGTDRAGSSGVRWVLDPLDGTASYVRG
                     YPGFGVSIGIEVDDRPSVGVVIDALGRITVGVAEWGAERDGVRARPSARTALADSVVA
                     TGFGYDAGMRVREAETLGRLIDRIADIRRSGSAAMDLCAVACGEVDAYYELHLAPWDT
                     AAGAVIVETAGAVFRQIDQPDGEVLTVAVPTQLLEPVLGLLGDAGLSV"
     gene            complement(2664..2873)
                     /locus_tag="VIH70_01945"
     CDS             complement(2664..2873)
                     /locus_tag="VIH70_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115157.1"
                     /translation="MKGQNLVTPQDVGKRVSFQFELPNGYVGEAVGILERYDHEAATY
                     LVRRKDDTIARVPARDVRFGKVIEP"
     gene            complement(2896..3978)
                     /locus_tag="VIH70_01950"
     CDS             complement(2896..3978)
                     /locus_tag="VIH70_01950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY8115158.1"
                     /translation="MAERVFPLPDLGEGLEEAIVSAWLVAEGDTVSLNQPFVEIETAK
                     ATVEVPAPFAGRVALLHVAAGETLAVGAPLATFEVVGAAEATSVAATPAVRKLARDLG
                     VDLSKISGSGPGGRVTREDVQAFARTGDEPAHVRRAIAATEIPVSPTRRAIADRLTEV
                     AAIPQVTTFRTVDCAALEAVRERLGVSPLPVFVRALANVCRDHPMLNARWEGERILLF
                     HEIRVGIATDTERGLVVPVLADAGSLGIAEIAAEIARLADAARSNELTRDDTVDATIA
                     VTNTGSYGSEFGTPLLNPGHAVTIGLGVIAPRALVIDGAVEARPACTLSLTFDHRVLD
                     GATVGRAFGALIELLESRERLDALPR"
     gene            complement(3978..4952)
                     /locus_tag="VIH70_01955"
     CDS             complement(3978..4952)
                     /locus_tag="VIH70_01955"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY8115159.1"
                     /translation="MAEHTMVAALNAALRDALAADPRTLVFGEDVGALGGVFRVTEGL
                     QAGFGRERVFDTPIAEAGIAGICIGLAMAGWRPIAEMQFDGFSYPALDQVISHLAKYR
                     ERTRGRVGVPAVIRIPSFGGIRGKEHHGESPETYYVHTAGLKVVVPSTPLDAYRLLRR
                     SIDDPDPVIFLEPKSRYWSKEDGDLTVEGPGIGRARVVREGGACVLIAYGAMVGRCLE
                     AATALADGGIEVAVLDLRSLVPLDVGAIGDAIRSSGRAVVVHEAPLTLGMGAEIVARI
                     VEEAFDHLEAPVVRVTGPDVPYPPASLEQRYLPSVERISNAVRTVVDY"
     gene            complement(4945..6006)
                     /gene="pdhA"
                     /locus_tag="VIH70_01960"
     CDS             complement(4945..6006)
                     /gene="pdhA"
                     /locus_tag="VIH70_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY8115160.1"
                     /translation="MKEIASTDVPIVRLVAPDGTAVGDVEVGLSEDERRELLRQMIRS
                     RRLDRECWALQRQGELTVYPPFEGQEAAQVGSAFALGPDDFVFPSFRELAAAVVRGVD
                     VVEYLQYHRGTWHGGPYDPIPSRFAPICVPVATQIVHAVGWAMGSKLDGNSACSLAYF
                     GDGSASEGDFHEGANLAAVFRAPAILFCQNNGWAISVPSNEQFAAPIAARAAGYGMRG
                     VRVDGNDVLAVYGVTRDAVGRARAGEGPTLIEAVTYRIGSHSTADDATRYRDEAEVAA
                     WRERDPIDRYRGFLLDAGTIDDAFVRGCDEEAEAWVAQIRAELTSLGEPPVAEMFDHA
                     FADPPPTLLRRREELLGDG"
     gene            6071..6871
                     /locus_tag="VIH70_01965"
     CDS             6071..6871
                     /locus_tag="VIH70_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115161.1"
                     /translation="MVPSAELRVYQPLEAFPPHEQAHWERHIVDGRLWLGPPRYRQEV
                     TSLGAGVLVPVGEDGAYVKVVEGRYHVCPWRTTLRVLAGMLSFREAAVFEDPSMFVTD
                     ASARRATRELRRLRRREPWQMSTIMHSPWHVPVRWFVLFDDDERRLLEVEGHHRLSYL
                     TTARKALRRAEDAVPVLRQSELGPIGELVVEMHQWLALFDPGSILELDYGGLCGMLTW
                     DEMDDDHSARDIHDALKALADKEFPRSADLYQSVLVRSAELRAHEGLN"
     gene            complement(6894..6966)
                     /locus_tag="VIH70_01970"
     tRNA            complement(6894..6966)
                     /locus_tag="VIH70_01970"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6931..6933),aa:Phe,seq:gaa)
BASE COUNT          974 a         2489 c         2512 g         1004 t
ORIGIN      
        1 ggcgctccgc gaagcaggcg cgaccgaggt gcacacccgc atcacctgcc cgccgatccg
       61 gtggccgtgc ttctacggca tcgacatgtc gacccggcag gagctgatcg ccgccgacct
      121 gaccgtcgag cagatcaggg actacgtcgg ggcggactcg ctcggatacc tgtcgctcgg
      181 ggggatggtg gctgccaccg ggcaggatgt gggctcgttc tgccgcgcct gcttcgacgg
      241 cgagtacccg atccctatcc ccgagcacgc cggcaagttc cttctcgagc accccgcgaa
      301 gtcgtgagct cggaggccta tcggcggtcc ggcgtggacc tggacgccgc cgcgagggcg
      361 gtggagctga tccgtgagct cgggggcggt gctacgcgac ccgagctcgt cgagggcgtc
      421 ggagggttcg ccgggttgtt cgagatcggc gagggacggt tcctcgccgc cgccacggac
      481 ggcgtcggca cgaagctcga gatcgcaagg cgctcgggcc ggctcgagac cgtcgggatc
      541 gacctggtgg cgatgtgcgc cgacgacgtc gtctgcaccg gcgccgaacc cctgttcttc
      601 ctcgattacc tcgccgtggg ctcggtcgac cccgagcgcg tggccgcgat cgtcgcgggg
      661 gtcgccgagg gttgccgacg cgcggggtgc gcgctgctgg gcggcgagac ggcggagcac
      721 cccggcgtga tggccgaaga cgcgttcgac ctcgcggggt tctgcgtcgg gatcgtcgag
      781 gaggaacgac ggctcgggcc ggaccgggtg cgggacgggg acgcgttgat cggtctcgcc
      841 tcgtcggggc tgcacgcgaa cggcttctcg ctggttcggg cggcgctcct ggtgcgggcc
      901 ggctacgccc tcgagcggat cgtgccgccc ttgcgccgcc cgctggtcga cgagctgctc
      961 gagccgaccg cgatctacgc gcccctcgtg ctcgagcttg cccgagaagg cgtgctgcac
     1021 gccgccgcgc atgtcacggg cggcggaatc gtcgagaacc tccggcgcgt cctcccggag
     1081 gggctcgagg ccgatgtcgc ctggaggtcg tggcccgaac ccccggtgtt cgagctgctg
     1141 cgcgcccaga ccggcgtgac cgagcacgac ctgcgggcga cgttcaacct cggcgtgggg
     1201 atggtgctcg tcgttccgcc cgccctggcc gagcgcgcga tcgcgcacgc gggcgggtcc
     1261 ggtcccgccg cgttcccgat cggagtcgtg cgagcctgac cggggtcggg catgggccgg
     1321 cgaccgtgcg tgggttggcg cggactgact agccgcctgt cgtccacgtg tcggggctcc
     1381 atccggccgg gggagaccag ccggtaccgc aaaaccggtc gcgaggccga tgcctcccgg
     1441 ccgaacgccg gccgataccg ttcctaggat gatgggcacg aagaccacgt cgccggttct
     1501 ggttgtcgat gacgagccac agatcgtctg gatgctccag ttcagcctcg aggcggaggg
     1561 ataccagacc ctctcggcgg ccgacggcat gacggcgctg gacgaggtgc gcgaacacca
     1621 cccgaaggtc gtcctgctcg acatcatgat gcccgtgatg gacggctggg ctttcctcga
     1681 ggagctgcag gcgatcccgg ccgccgagcg tccccgcgtg atcgtggtct cggcgcgggc
     1741 gagcctgcgc gaccgcgcga aggcggccga gctcggagcc gacgcattcg tcgccaagcc
     1801 gttcagcgtc gacgacctgc tggtcgtcct gcacgacgtc gaacgggcgg tctgagaccc
     1861 gacgcgtcag acgctcaggc ccgcatcacc gagcagcccg aggaccggtt cgagcagctg
     1921 cgtggggacc gcgacggtga gcacctcgcc gtcgggttgg tcgatctgcc ggaacacggc
     1981 gcccgcggtc tccacgatca ccgcgcccgc ggccgtgtcc cagggcgcga ggtggagctc
     2041 gtagtaggcg tcgacctcac cgcaggccac cgcgcacaga tccatcgccg ccgacccgct
     2101 ccgccgtatg tccgcgatgc gatcgatgag ccgcccgagc gtctcggcct cgcggacccg
     2161 catccccgcg tcgtacccga accccgtggc cacgacggaa tcggccagcg cggttcgggc
     2221 cgacggccgt gcgcgaacgc cgtcgcgttc ggctccccac tcggccacgc ccaccgtgat
     2281 ccggcccagg gcgtcgatca cgacgccgac gctcgggcgg tcatcgacct cgatcccgat
     2341 cgatacgccg aagccggggt atccgcgcac gtagctcgcc gtcccgtcga gcgggtcgag
     2401 gacccaccgc acgccggatg agccggcccg gtccgtccct tcctcgccga ggatcgcgtc
     2461 gtccggccgc gcgtcgagga tcccctcgac gagggcgtgc tcggcggccc ggtcgatctc
     2521 ggtgaccaga tcgccagcgg cggacttccg gtcgatttcg aaccctccga ggagcccgcc
     2581 ccgctggatc tccgccgctc ggtgggcgag tcccaccgcg agcgttcgga gctcgtcgag
     2641 cagctcggcg gatcctgccg ggatcagggc tcgatgacct tgccgaaccg gacgtcgcgg
     2701 gccgggacgc gcgcgatggt gtcgtccttg cggcggacca ggtaggtcgc ggcctcgtgg
     2761 tcgtaacgct cgagtatgcc gaccgcctct ccgacgtagc cgttcggcag ctcgaactgg
     2821 aaggagacgc gcttgccgac gtcctgcggc gtcacgaggt tctggccctt cacgagccgg
     2881 aggatatcgg accgctcagc ggggcaacgc gtccaggcgt tcgcgcgact cgaggagctc
     2941 gatcagcgcg ccgaacgccc gtcccacggt cgcgccgtcg agcactcggt ggtcgaacgt
     3001 gagcgagagc gtgcacgccg gacgggcctc gaccgcgccg tcgatcacga gtgccctcgg
     3061 ggcgatcacg ccgaggccga tcgtgaccgc gtggccgggg ttcagcagcg gcgtcccgaa
     3121 ctcggagccg tagctgccgg tgttcgtgac ggcgatcgtc gcgtcgacgg tgtcgtcgcg
     3181 ggtcaactcg ttcgagcgtg ccgcgtccgc gaggcgtgcg atctcggcag cgatctcggc
     3241 gatcccgagc gaaccggcgt cggcgagcac gggaaccacc aatcccctct ccgtgtccgt
     3301 ggcgatcccc actcgtatct cgtggaacag gaggatccgc tcgccctccc atctcgcgtt
     3361 cagcatgggg tggtcccggc acacgttggc gagcgcccgg acgaacaccg gcaggggtga
     3421 gacgccgagg cgctcgcgaa cggcctcgag cgccgcgcag tccacggtcc ggaacgtggt
     3481 cacctgcggg atggccgcga cctccgtgag ccggtccgcg atcgcccgtc gcgtgggcga
     3541 cacggggatc tcggttgccg caatcgcccg ccggacatga gcgggttcgt cgccggtacg
     3601 tgcgaacgcc tggacgtcct cgcgcgtcac gcgtccgccc ggaccggagc ccgagatctt
     3661 cgacaggtcg acgcccaggt ccctcgccag tttgcggacg gccggcgtgg cggcgaccga
     3721 cgtcgcctcc gccgctccta cgacctcgaa cgttgcaagc ggcgccccga cggcgagcgt
     3781 ctcgcccgcc gcgacgtgga ggagcgccac gcggccggcg aacggcgcgg gcacctcgac
     3841 ggtcgccttg gccgtctcga tctcgacgaa cggctggttc agcgagaccg tgtcaccctc
     3901 ggcgacgagc cacgcgctca cgatcgcctc ctcgagaccc tccccgagat cgggaagggg
     3961 aaagacgcgc tcggccatca gtagtcgacc accgtgcgga cggcgttcga gatccgttcg
     4021 acgctcggga ggtagcgctg ttcgagggag gcgggcgggt agggcacgtc cggtccggtg
     4081 acgcgcacca cgggcgcctc gaggtgatcg aacgcctcct cgacgatccg cgccacgatc
     4141 tcggcgccca tccccagcgt caggggcgcc tcgtgcacga cgaccgcacg cccgctcgac
     4201 cggatggcgt cgccgatcgc cccgacgtcg agcgggacca gcgatcggag gtcgaggacg
     4261 gcgacctcga tcccgccgtc ggcaagggcg gtcgcggcct cgaggcatcg gccgaccatc
     4321 gcgccgtagg cgatcaggac gcaggcccct ccctcccgca cgaccctcgc ccgtccgatg
     4381 cctggtccct ccacggtcag gtcgccgtcc tcctttgacc agtaacggct cttcggctcg
     4441 agaaagatca cggggtccgg atcgtcgatc gagcgccgca gcagccggta ggcgtcgagc
     4501 ggcgtcgacg gcaccacgac cttcagcccg gcggtgtgca cgtagtaggt ctcggggctc
     4561 tcgccgtgat gctccttgcc ccggatgccg ccgaacgacg ggatgcggat cacggcgggc
     4621 acccccaccc ggcctcgggt gcgttcgcgg tacttcgcga ggtgactgat cacctggtcg
     4681 agcgcggggt acgagaagcc gtcgaactgc atctcggcga tcgggcgcca cccggccatc
     4741 gcgaggccga tgcagatccc ggcgatcccg gcctcggcga tcggcgtgtc gaatacccgc
     4801 tcccggccga acccggcctg caggccctcg gtcacacgga agacccctcc gagcgcaccg
     4861 acgtcctcgc cgaacacgag ggtacgtggg tcggcggcga gcgcatcgcg gagcgccgcg
     4921 ttcagcgccg cgaccatcgt gtgctcagcc atcgccgagg agctcctcac gccggcggag
     4981 caacgtcggc ggcgggtcgg cgaacgcgtg gtcgaacatc tcggccaccg gtggctcgcc
     5041 gagcgaggtg agctcggcgc ggatctgggc cacccaggcc tcggcctcct cgtcgcaccc
     5101 gcggacgaag gcgtcgtcga tcgtgccggc atcgagcagg aagccccggt agcggtcgat
     5161 cgggtcgcgt tcgcgccagg ccgcgacctc ggcctcgtcg cggtagcggg ttgcgtcgtc
     5221 ggcggtcgag tgcgagccga tgcggtatgt caccgcctcg atcagcgtgg ggccctcacc
     5281 cgctcgggca cgtccgaccg cgtcccgcgt gacgccgtac accgccagga cgtcgttgcc
     5341 gtcgacccga accccccgca tgccgtagcc ggccgcccgg gccgcgatcg gggcggcgaa
     5401 ctgctcgttc gaggggaccg agatcgccca gccgttgttc tggcagaaca ggatcgcggg
     5461 cgcgcggaag acggcggcga ggttcgcgcc ctcgtggaaa tcgccctcgg aggcggagcc
     5521 gtcgccgaag tacgcgaggg agcaggcgga gttcccgtcg agcttggagc ccatcgccca
     5581 cccgaccgcg tggacgatct gcgtcgcgac ggggacgcag atcggggcga acctgctggg
     5641 gatcgggtcg tacggtccgc cgtgccatgt cccgcggtgg tactggaggt actcgaccac
     5701 gtcgacgccc cgcacgaccg ccgccgcgag ctcgcggaac gacgggaaca cgaagtcgtc
     5761 ggggccgagc gcgaacgcgc tgccgacctg cgccgcctcc tggccctcga acggcgggta
     5821 gacggtgagc tcgccctgac gctggagcgc ccagcactcg cggtccagcc gccgcgaacg
     5881 gatcatctgc cgcaggagct cgcggcgctc gtcctcggac agccccacct cgacgtcgcc
     5941 gaccgcggtg ccatcgggcg cgacgagccg gacgatcggg acgtcggtgg acgcgatctc
     6001 cttcatgcgg ggcggcctcc ggcgttcact ggcgagcggg gccaggggag catatataca
     6061 ctcgaacgcc gtggtcccgt cggccgagct ccgcgtctac cagccgctcg aggcgttccc
     6121 gccacacgag caggcgcact gggagcggca catcgtcgac gggcgtctgt ggctcgggcc
     6181 gccgaggtat cggcaggagg tgacctcgtt gggcgcgggg gtcctggtgc ccgtgggcga
     6241 ggacggcgcc tacgtcaagg tcgtcgaggg ccggtaccac gtgtgtccgt ggcggacgac
     6301 gctccgcgtc ctcgccggca tgctgtcgtt ccgcgaggcg gccgtgttcg aggacccttc
     6361 gatgttcgtc acggacgcga gcgctcgccg cgcgacgcgc gagctccgcc ggctgcgacg
     6421 ccgggaaccg tggcagatgt cgacgatcat gcacagcccc tggcacgtgc cggtccggtg
     6481 gttcgtcctg ttcgacgacg acgagcgtcg gctcctcgag gtcgaggggc atcaccggct
     6541 gtcctacctc acgacggcaa ggaaggcgct ccgccgggcg gaggacgccg tgccggtgct
     6601 ccgccagtcc gagctcggtc cgatcggcga gctcgtggtc gagatgcacc agtggctcgc
     6661 gctgttcgac ccggggtcga tcctcgagct cgactacggt gggctgtgcg ggatgctcac
     6721 gtgggacgag atggacgacg accacagcgc ccgggacatc cacgatgcgc tcaaggcgct
     6781 cgccgacaag gagttcccgc gctcggccga cctgtaccag tcggtgctgg tgcgctccgc
     6841 cgagctccgg gcgcacgagg gcctgaactg acacggcgcg tggccaggcc gcgtggccag
     6901 gggcagaatc gaactgccga caccgcggtt ttcagccgcg tgctctgcca actgagctac
     6961 ctggcctcca gagccgaac
//
LOCUS       DATBRK010000063         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2607774, whole genome shotgun sequence.
ACCESSION   DATBRK010000063 DATBRK010000000
VERSION     DATBRK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2607774"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /gene="hutH"
                     /locus_tag="VIH70_01975"
     CDS             <1..1515
                     /gene="hutH"
                     /locus_tag="VIH70_01975"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY8115162.1"
                     /translation="VGESLSLEDVVAVARGAEVELGPDATSRLEAARDVVERTVAANR
                     TVYGVTTGFGSLANERIEPDQADALQLGIVRSHATAVGPPLSREEARAMLLLRAHVLA
                     LGHSGVRAEVVERMVEMLNRDLIPVVPQQGSLGASGDLAPLANLALPLVGRGELLVDA
                     TPQPAGGVLRSAGLEPLRLKAKEGLALVNGTQGMLAIGILSVARAERLVRTADVAAAM
                     SVEAALGSDRVFDKRLTALRPHPGVVASASNLRRLLEGSAIVASHRDSTHLVQDAYSL
                     RCSPQVNGATREVLRFVRGVLEVEANSVSDNPIVLPDDDEVLSGGNFHGQPVAVALDA
                     LAAALVPLASIAERRLYRLLDPARNNGLPAFLVAESGLNSGFMLVQYTAASLVSECKT
                     LAHPASVDSIGSSAGQEDHVSMGMTAARHAREVLSNAEVVIALEALVAAQALDLRAPL
                     EPSAATGAARDAIRTRVAFLDADRELGSDIAAATDLLRTGALLESVEGTLGRLD"
     gene            complement(1532..2689)
                     /locus_tag="VIH70_01980"
     CDS             complement(1532..2689)
                     /locus_tag="VIH70_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY8115163.1"
                     /translation="MKRFAGLLLVVTVVLLVPTTAHAQTGVTITMSASSGRITFGEQV
                     RLSGSSTGAPAGSTVEIRNAADVAVASATTNAAGDFSAGLEPWGSDSYVAVLGDGVSG
                     PVNVGVRAVVSARMGQVRLFDHVVVRGRVGPARPGSSVVVQLIKDGRTIERRPVMMGS
                     AGGFRTRFTIPKPGMYRARASFSAPDLLRGSARTHADATPLPYLSSGSSGRFVRLLEG
                     RLVDLHYRLVGTKDGRYDFRTADAVVAFHKVQRMDRSDAVNEATWRRLASPIRPQARR
                     TWQDFHFEVDQTKQVLYTVEDGDITNVLHVSTGAGGLTRDGAFRVRAKIAGFSPNHLY
                     YPSYFDGYRALHGWTEVPTYAASHGCVRIPYWNAQWVYGLADYGTPVVIYH"
     gene            complement(2686..>2967)
                     /locus_tag="VIH70_01985"
     CDS             complement(2686..>2967)
                     /locus_tag="VIH70_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8115164.1"
                     /translation="TDFNPGTSPVLSMPEAIAIACSLYGLRPLEALAAATMNASAALD
                     LAPQRGTLSPGRPADLVVLDGDAFRLVPYRPGHNPVVRTFVDGERVGGR"
BASE COUNT          420 a         1036 c         1090 g          421 t
ORIGIN      
        1 gtcggggagt cgctgtcgct cgaggacgtg gtcgcggtcg cgcgcggggc cgaggtcgag
       61 ctcggtccgg acgcgacgag ccggttggag gcggcacgag acgtcgtcga gcgcaccgtc
      121 gcagccaacc ggacggtcta cggcgtgacg accgggttcg gctcgctcgc gaacgagcgc
      181 atcgagccgg accaggcgga cgcgctccag ctcgggatcg tgcgttcgca cgcgaccgcc
      241 gtcggaccgc cgctctcgcg cgaggaggcg cgcgcgatgc tgctgctccg cgcgcacgtg
      301 ctcgcgctgg ggcactcggg ggtgcgagcc gaggtagtcg agcgcatggt cgagatgctg
      361 aaccgcgacc tgatccccgt cgtcccgcag caggggtcgc tcggcgcgtc gggcgacctg
      421 gccccgctcg cgaacctcgc gctcccgctc gtcggccgcg gcgagctgct cgtcgacgca
      481 acacctcagc ccgccggcgg cgtccttcgg tcggccgggc tcgaaccgct ccggctcaag
      541 gccaaggagg gactcgcgct cgtgaacggg acgcagggca tgctcgcgat cgggatcctc
      601 tccgtggcgc gcgccgaacg gctcgtgcgg acggccgacg tcgcggccgc catgagcgtg
      661 gaggcagcgc tgggctcgga cagggtcttc gataagcggc tcacggcgct ccgcccgcac
      721 ccgggggtgg tcgcctccgc gtcgaacctc cgtcggctcc tggaggggag cgcgatcgtg
      781 gcgtcgcacc gcgacagcac gcacctcgtg caggacgcgt attcgctccg atgctcgccg
      841 caggtgaacg gggcgacgcg cgaggtgctc cggttcgtcc gcggcgtcct cgaggtggag
      901 gcgaactccg tctcggacaa cccgatcgtg ctgccggacg acgacgaggt gctctcgggt
      961 gggaacttcc acggccaacc ggtcgcggtc gcgctcgacg cgctcgcggc ggcgctcgtc
     1021 ccgctcgcga gcatcgccga gcggaggctc taccggctgc tcgatccggc gcggaacaac
     1081 gggctgccgg cgttcctggt cgccgagagc ggcctgaact cgggcttcat gctcgtgcag
     1141 tacacggcgg cgtcgctcgt ctccgagtgc aagacgctcg cgcaccccgc ctcggtcgat
     1201 tcgatcggtt cgagcgcggg gcaggaggac cacgtgagca tggggatgac ggccgcccgc
     1261 cacgcgcgcg aggtgctctc gaacgccgag gtcgtgatcg ccctcgaggc cctggtggcg
     1321 gcgcaggcgc tcgacctgcg cgcgccgctc gagccgtccg ccgcgacggg agccgctcgg
     1381 gatgccatcc gcacgcgggt cgcgttcctc gacgccgacc gcgagctcgg ttcggacatc
     1441 gccgccgcga cggatctcct gcgcacgggt gcgttgctcg agtccgtgga gggaaccctg
     1501 ggtcgactgg actgatcgcg tccggacgcg atcagtggta gatcacgacg ggggtgccgt
     1561 agtcggcgag gccgtacacc cactgcgcgt tccagtaggg gatccgaaca cagccgtggc
     1621 tggccgcgta ggtgggcacc tcggtccagc cgtggagggc ccgatatcca tcgaagtagc
     1681 tcgggtagta gaggtggttc gggctgaagc cggcgatctt cgcccgcacg cggaacgcgc
     1741 cgtcgcgggt caggccgccg gctccggtcg agacgtggag cacgttcgtg atgtcgccgt
     1801 cctccaccgt gtagagcacc tgcttggtct ggtcgacctc gaaatggaag tcttgccagg
     1861 tgcggcgggc ctgcggtcgg atcggacttg cgagccttcg ccaggtggcc tcgttcaccg
     1921 cgtccgaacg gtccatccgc tgcaccttgt ggaaggcgac gacggcgtcg gcggttcgga
     1981 agtcgtaccg gccgtccttg gtcccgacga gccggtagtg gaggtcgacg agccgcccct
     2041 cgagcagcct cacgaagcga cccgacgaac ccgacgacag gtacggaagc ggcgtggcat
     2101 ccgcgtgggt gcgcgcgctg cccctcagca ggtcgggtgc cgagaacgat gccctcgccc
     2161 ggtacatgcc gggcttgggg atcgtgaacc tcgtgcggaa cccaccggcc gagcccatca
     2221 tcacggggcg gcgttcgatg gttcggccgt ccttgatcag ctggaccacg acggaggagc
     2281 ccggccgcgc gggaccgacc ctgccgcgga cgacgacgtg gtcgaacagc cggacctgtc
     2341 ccatccgagc cgagaccacc gcgcggaccc cgacgttcac gggtccgctc acgccgtcgc
     2401 cgagcacggc cacgtagctg tcggaccccc acggctcgag cccggccgag aaatcgcccg
     2461 ccgcgttggt ggtcgccgac gcgaccgcca cgtcggcggc gttacggatc tcgaccgtgg
     2521 atccggcggg tgcccccgtc gacgaccccg agagcctcac ctgctcgccg aatgtgatcc
     2581 tgccggagga cgccgacatc gtgatagtga cgcccgtctg ggcgtgggcc gtggtcggga
     2641 cgagcagcac gaccgtgacc accagcagga gcccggcgaa ccgcttcaac gtcctccaac
     2701 cctctcgccg tcgacgaacg tccggaccac cgggttgtgg cccggccggt acggcaccag
     2761 ccggaaggcg tcgccgtcga ggaccacgag gtcggcgggc ctgcccggcg aaagcgtacc
     2821 ccgctgcggc gcgagatcga gtgcagcgga ggcattcatc gtcgcggccg cgagggcctc
     2881 gagcggtcgg agcccgtaga gcgaacacgc gatcgcgatc gcctcgggca tcgagagcac
     2941 cggcgaggtg ccggggttga agtcggt
//
LOCUS       DATBRK010000064         4806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2750812, whole genome shotgun sequence.
ACCESSION   DATBRK010000064 DATBRK010000000
VERSION     DATBRK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2750812"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1819
                     /locus_tag="VIH70_01990"
     CDS             <1..1819
                     /locus_tag="VIH70_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY8115165.1"
                     /translation="DREERPDLDAIYMDAVQTMTGGGGWPLTAFLTPQGKPFYAGTYY
                     PNQARHGLPAFRTVLSAIADVWANRRSEAEEQGSRVTEAIERSTGLGASPQPLTEAIL
                     ATAFGGLRRAFDARWGGFGGAPKFPQPMTMEFCLRMHLRGTPDALEMVTITLDKMAGG
                     GMYDQVGGGFHRYSTDERWHVPHFEKMLYDNALLIRLYVHAWQVTGFERYRRVATETC
                     EYLLRELRHAEGAFFSSQDADSEGVEGRFFVWPWDELVGIAGEAVATTFGATPGGNWE
                     GTNVLWRPLPVEAVAAETELDAGELADELETARAELFEIREGRTHPATDDKILTAWNG
                     LAIAALAEAGRAFGEVDYVDAAVRAADFVLTHLRDDTGRLMRSWRNGKAGGPAFADDH
                     AAMADACLVLYETTFELRWFEQARALADELVRLFHDEVRGGFFLVGRDAEPLVVRQKE
                     LSDNATPSGNSASADVLLRLAHLTGEASYEAAGEGALRLVRDAMAGAPSGFGHALGAL
                     DAYLSAVREVAIVGDPGSKETLALVAEVTTNRFLPNHVLAVSSPGDEASSAAVALLRD
                     RPQTDGRATAYVCERFQCLLPVTDPAALAAQVGGTAIEG"
     gene            1832..2938
                     /locus_tag="VIH70_01995"
     CDS             1832..2938
                     /locus_tag="VIH70_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115166.1"
                     /translation="MIVLRVLLFLAGLALVVLMIRSMVRTFLVPRALQAHLSRAVFVT
                     VRRVFRLRASERRDYAIRDRVMALYAPVALLVLLAVWFAILLAGFSAMFFALGEVSPF
                     EAFTLAGSSMLTLGFARPPDDAGVVLSFTAAGLGLVLLALFITYLPSLYGAFQRRERG
                     VAKLEVRGGQPPSGVYLIELAWAVGRLENLRALWAEWEDWFTDVDETHSSFPSLVFFR
                     STHPDQSWITAAGAILDGAALFVSTVDTPRQPEPEFMIRSGYLCLRHLAEFFNIPIDH
                     DPRPDDPISITREEFEAAAERLRASGVPLRPDRDRSWRDFAGWRVNYDAALIALAKLS
                     MAPRAPWSSDRYEDVHFVPPALPWLRRSGTGTRG"
     gene            2972..3481
                     /locus_tag="VIH70_02000"
     CDS             2972..3481
                     /locus_tag="VIH70_02000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8115167.1"
                     /translation="MTEATFVTSMGSFTARLMPEHAPKTVANFVELARGSKGWTDPRD
                     GSHATDPLYAGTIFHRVIGDFMIQGGDPTGTGTGGPGYTFEDETPPAGPRFDRPGLLA
                     MANAGPNSNGCQFFVTVAAADWLSGKHTIFGEVTDGYDVVEAISRTPTKAQDRPVTDV
                     VLERVDITD"
     gene            complement(3530..4462)
                     /locus_tag="VIH70_02005"
     CDS             complement(3530..4462)
                     /locus_tag="VIH70_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115168.1"
                     /translation="MRRVSFIATAVLVLLGLAPTGAGALPSALASSNGVLAFRSDRDG
                     EPDLFTLDPSTFAVTKLTTRSGASELQPAWSPDGGRIAYVRRAGTIGRADIWFMKASG
                     RGRTRVTSTAVPERDPSWSPDGTHLIYAARTGPGEPFRIFVANDDGSGLHQLTTQSAG
                     SADRSPVWSPDGSRIAFVSNRAGGFPELYLMSANGTGVVRLTTNSFIDANPSWSPDGT
                     RLVFERCCENGTSDLFTIDVATRAEVNLTPSTAQQEFDPTWSPDGTRIAFAAFDVGQG
                     NLDIWVMNADGTAPMRLTQEAGPDLSPHWQPIPV"
     gene            4517..>4806
                     /locus_tag="VIH70_02010"
     CDS             4517..>4806
                     /locus_tag="VIH70_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115169.1"
                     /translation="MAHLAVAVLLVLAIAPAQDAGAGRPAPQARAPIVTTRKIAPGLK
                     YIRIVRRQVPLRTFVLSIDLSKAITLDTTIADDSLPSRRQLSRIVRNHGALAG"
BASE COUNT          691 a         1702 c         1692 g          721 t
ORIGIN      
        1 cgaccgggag gaacgcccgg acctggacgc gatctacatg gacgccgtcc agacgatgac
       61 cggcggaggg ggatggcccc tcaccgcgtt cctgaccccg cagggcaaac cgttctacgc
      121 cggcacctac tacccgaacc aggcccgcca cggcctcccc gccttccgga cggtcctgtc
      181 ggccatcgcg gacgtgtggg ccaaccgccg ctccgaggcc gaggagcagg gctcgagggt
      241 caccgaggcg atcgagcggt cgaccggcct cggggcctcc ccgcagccac tcaccgaagc
      301 gatcctcgcg acggccttcg gcgggctccg gcgagcgttc gacgcgcgct ggggagggtt
      361 cggcggcgcg ccgaagttcc cccagcccat gaccatggag ttctgccttc gcatgcacct
      421 gcgtgggacg cccgacgcgc tcgagatggt gacgatcacg ctggacaaga tggcgggcgg
      481 cgggatgtac gaccaggtgg gcggcggctt ccatcggtac tcgaccgacg aacgatggca
      541 cgtcccccac ttcgagaaga tgctgtacga caacgcgctg ctgatccgtc tgtacgttca
      601 cgcctggcag gtgaccggct tcgagcgcta ccgccgcgtc gccaccgaga cctgcgagta
      661 cctgctccgc gagctccggc acgccgaggg ggcgttcttc tcctcccagg acgcggacag
      721 cgagggcgtc gagggcaggt tcttcgtctg gccgtgggac gagctcgtcg ggatcgcggg
      781 cgaggcggtc gcgaccacgt tcggggcgac accgggcggc aactgggagg gcacgaacgt
      841 gctgtggcgc ccgctgccgg tcgaggcggt ggccgcggag acggagctcg atgccggaga
      901 gctggccgac gagctcgaga cggcgagggc cgagctgttc gagatccgcg agggccgaac
      961 ccacccggcg accgacgaca agatcctgac cgcctggaac ggcctggcga tcgccgccct
     1021 ggcggaggcc gggcgggcgt tcggcgaggt cgactacgtg gatgccgccg tccgcgccgc
     1081 ggacttcgtc ctcacgcacc ttcgggacga tacggggcgg ctgatgcgat cgtggcggaa
     1141 cggaaaggcc ggcggacccg cgttcgccga cgaccacgcg gcgatggcgg acgcatgcct
     1201 cgtcctgtac gaaacgacct tcgagctccg atggttcgag caggcgcgag cgctggccga
     1261 cgagctcgtg cgtctgttcc acgacgaggt tcgcggcggc ttcttcctgg tgggacgtga
     1321 cgcggagccg ctagtcgtcc gtcagaagga actctcggac aacgcaacgc cgagcggcaa
     1381 ctccgcctcg gccgacgtcc tgctgcgcct cgcccacctg acgggcgagg cctcctacga
     1441 ggcggcgggc gagggcgcgc tgcgcctggt ccgggacgcg atggcggggg cgccgtcggg
     1501 gttcgggcat gcgctcgggg ccctcgacgc ctacctgtcc gccgtcagag aggtggcgat
     1561 cgtcggagac cccgggtcga aagagaccct ggccctggtc gccgaggtca ccacgaaccg
     1621 cttcctcccc aaccacgtcc tcgccgtttc ctcccccggc gacgaggcct cgagcgccgc
     1681 ggtcgcgctc ctacgggacc gcccgcagac ggacgggcgc gccacggcct acgtgtgcga
     1741 gcgcttccag tgcctgctcc ccgtcacgga tccggccgca ctcgcggcgc aggtcggcgg
     1801 gacggccatc gaggggtagg ctcgcccgaa cgtgatcgtc ctccgggtgc tcctgttcct
     1861 cgcgggcctc gccctcgtcg tgctcatgat ccgctcgatg gtgcggacgt tcctggtccc
     1921 ccgcgcgctc caggcccatc tgtcccgcgc cgtcttcgtg acggtccgcc gcgtgttccg
     1981 actgcgggcg agcgagcgcc gcgactacgc gatccgcgat cgggtgatgg cgctctacgc
     2041 gccggtcgcg ctgctcgtcc tgctcgccgt ctggttcgcg atcctgctgg ccggcttctc
     2101 ggcgatgttc ttcgcgctgg gcgaggtgag cccgttcgag gcgttcacgc tggccgggtc
     2161 gtcgatgctg accctgggct tcgcgaggcc ccccgacgac gccggcgtcg tcctttcctt
     2221 cacggccgcc gggctcggcc tggtgctcct cgctctgttc atcacctacc tgccgagcct
     2281 gtacggggcc ttccagcgac gcgagcgcgg cgtggccaag ctcgaggttc ggggcgggca
     2341 gccgccgagc ggcgtctacc tgatcgagct ggcctgggcc gtggggcggc tggagaacct
     2401 ccgcgcgctc tgggccgagt gggaggactg gttcaccgac gttgacgaga cccacagctc
     2461 gttcccgtcg ctcgtgttct tccgctccac gcatcccgat cagtcgtgga tcacggccgc
     2521 cggtgcgatc ctggacggtg cggcgctgtt cgtctcgacg gtcgacacac ctcgtcagcc
     2581 cgaacccgag ttcatgatcc ggtcgggata cctgtgtctc cggcatctcg ccgagttctt
     2641 caacatcccg atcgaccacg atcccaggcc cgacgacccg atctcgatca cgcgggagga
     2701 gttcgaggcg gcggccgagc gcctgcgggc gtccggcgtc ccgctgcgac cggaccggga
     2761 tcggagctgg cgggacttcg ccggctggcg cgtgaactac gacgcggcgc tgatcgcgct
     2821 cgccaagctc tcgatggccc cgcgcgcgcc ctggtcctcg gaccgctacg aggacgtgca
     2881 cttcgtgccg ccggccctcc cctggctccg acggtccggg acagggaccc gcgggtagaa
     2941 tgcccgctcc gcgcaagggg ggctccgccc gatgaccgag gcgacgttcg tgacatccat
     3001 gggttcgttc accgcccgac tgatgcccga gcacgcgccg aaaaccgtcg ccaacttcgt
     3061 ggaactcgcg cgggggtcga agggatggac cgatccgcgc gacgggagcc acgcgaccga
     3121 cccgctctac gcggggacga tcttccaccg cgtgatcggc gacttcatga tccagggggg
     3181 cgacccgacc ggaacgggca ccggaggccc cggctacacg ttcgaggacg agaccccacc
     3241 cgccgggccg cgtttcgacc gtccggggct gctcgcgatg gccaacgccg gaccgaactc
     3301 caacggctgc cagttcttcg tgacggtcgc cgccgccgat tggttgtccg gtaagcacac
     3361 gatcttcgga gaggtcaccg acggctacga cgtggtggag gcgatctcgc ggaccccaac
     3421 gaaggcccag gaccgcccgg tcaccgacgt cgtcctggag cgcgtcgaca tcaccgactg
     3481 acccggcgac ctcctgacgc cgaccgggtc gaccttccga tccgatcgat cacacgggga
     3541 tcggctgcca atggggcgag aggtcgggtc cggcctcctg tgtgagccgc atcggagcgg
     3601 tcccgtcggc gttcatgacc cagatgtcga ggttcccctg gccgacatcg aacgccgcga
     3661 acgcgatccg cgtgccgtcg ggcgaccagg tcggatcgaa ctcctgctgc gccgtcgagg
     3721 gggtgaggtt cacctcggcc cgcgtcgcga cgtcgatggt gaacaggtcg gaggtgccgt
     3781 tctcgcagca gcgttcgaac accagccgcg tcccgtcggg cgaccacgag gggttcgcgt
     3841 cgatgaacga gttggtcgtg aggcgcacga cgccggtccc attcgcggac atcagataga
     3901 gctcggggaa cccgccggcg cggttggaga cgaacgcgat acggctcccg tcgggcgacc
     3961 acacgggcga ccggtcggcc gagccggcgg actgggtcgt cagctggtgg agcccggaac
     4021 cgtcgtcgtt ggcgacgaag atccggaacg gctcgcccgg ccccgtgcgc gccgcgtaga
     4081 tcaggtgggt gccgtccggc gaccaggaag ggtctcgctc ggggacggcg gtcgaggtca
     4141 ccctcgtgcg gcccctcccg gaggccttca tgaaccaaat gtccgcgcgg ccgatggtcc
     4201 cggcccgacg aacgtaggcg atccgcccgc catccggtga ccaggccggc tgcagctcgg
     4261 acgcgccgga acgtgtcgtg agcttggtga cggcgaacgt agagggatcg agggtgaaca
     4321 ggtcgggctc gccgtcccga tccgatcgga acgcgagcac cccgttgctc gaggccaagg
     4381 cggagggcag cgcgccggcc ccggtcggag cgagcccgag gagcacaagg accgcggtcg
     4441 cgatgaagct gactcgtcgc acggttcccc tttaggatgt gccgtcgtga aacctgtgca
     4501 ccctaccatc cgcgtcgtgg cccacctggc cgtggcagtg ctcttggtgc tcgcgatcgc
     4561 tcccgcgcag gacgccgggg caggtcgccc ggcgcctcaa gcacgcgccc ccatcgtgac
     4621 gacccggaag atcgccccgg gcctgaagta catccgcatc gtgcggcgcc aggtcccgct
     4681 ccggacgttc gtgctgagca tcgacctgtc gaaggccatc acgttggaca ccacgatcgc
     4741 ggacgactcc ctgccgtcgc gtcggcagct ctcgaggatc gtgcggaacc acggggcgct
     4801 cgccgg
//
LOCUS       DATBRK010000065         8719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1305896, whole genome shotgun sequence.
ACCESSION   DATBRK010000065 DATBRK010000000
VERSION     DATBRK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305896"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /gene="otsB"
                     /locus_tag="VIH70_02015"
     CDS             <1..510
                     /gene="otsB"
                     /locus_tag="VIH70_02015"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY8115170.1"
                     /translation="YVGLYGLEDGASELVTALVPRAEAAASEVPEAWVEDKGVSVAVH
                     YRQAPDPASARAALLVALQPVATEAGLEIIQGKMVVELVPPERPLKGGAVERLVGEHA
                     LQAVLYAGDDHADLDAFAALDRLSASGEVGVTVRVAVRGPETPPAMIEAADVVADGPE
                     GVVDLLRTL"
     gene            complement(507..1970)
                     /locus_tag="VIH70_02020"
     CDS             complement(507..1970)
                     /locus_tag="VIH70_02020"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY8115171.1"
                     /translation="MSPPIIVASNRGPLTFERAKGGGFQARRGSGGLVTALSSALIEA
                     EGVWVAAAMSEGDREMVAASKGGRIDHAEGEVRYRLRYLDIPPEIYDGYYNRVSNGML
                     WFAYHYLWDTVTSPSWGSDVEQAWDAYVDVNRRFATALAEEGDALGGDTAFLIQDYHL
                     SLVPRILRELVPNALIAHFSHTSIAGPTYFRMLPARIHDQVLGGLMGADVLGFHSPAW
                     AENFLLSARQFPGARVDLARSRIVTEGREVTVRIHPISVDGRAMREEAATPRVRSLRH
                     ALNRWRDNNRLILRVDRLELTKNVVRGFQAYELFLRRNPSWIGRVRFLALLSPSRMEL
                     PEYREYTEACLDEARRVNDALGRDGWLPIELRLKDDYIGAIAAYGLYDVLFVNPVIDG
                     MNLVAMEGPLLNRRRGVLVLSRNAGAYGRLGRYALPVNPFDVDEMADALEQAIAMPVD
                     ERTRRSRGLSRLVLANPPARWVGGQLEDLERAKRRRR"
     gene            complement(1967..3211)
                     /locus_tag="VIH70_02025"
     CDS             complement(1967..3211)
                     /locus_tag="VIH70_02025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8115172.1"
                     /translation="MLPIVPVQPKRVSEYAEAAGQEALDSLREAARQLQGVRVLNVSS
                     TAYGGGVSELLQTQVPLMNDLGVDTTWVVIEGSDRFFNVTKAVHNALQGAETHLDEDM
                     RETYWERMHANAAEFPDDFDVVLVHDPQPAALLQVLEEGSRRKGKWIWRCHIDLSAPY
                     RPVWDFFEPIVNRYDAAIFTAEEYVQPGVQGPVRAFIPPSIDPLSPKNAPMMESTWQG
                     VLAPFGVDPDRPLVTQVSRFDPWKDPLGVIDAFRLIRREVPDAQLALVGSLAHDDPEG
                     IRYLDLTTEHAAGEPDIHLLTNLSGVGDSAVNAFQRASNVIVQKSLREGFGLVVAEGM
                     WKWKPVVGGDVGGIRLQIQDGVTGFLVNGIGETAQHVVELLRDPERGKAMGEAGRELV
                     RARFLCLREIEDTLRLISRLTV"
     gene            complement(3252..3668)
                     /locus_tag="VIH70_02030"
     CDS             complement(3252..3668)
                     /locus_tag="VIH70_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8115173.1"
                     /translation="MRVLVIDDEPDVLMLCRLNLELAGHEVIEATNGEAGLELALHEQ
                     PDVVVLDVMLPRLDGISVLGTLANDEQTRDLPVILLTAKTQLEDRRAGWRAGCTEYLT
                     KPFSPVDLVGIAERARETSAVERSDRRQRELAQLGA"
     gene            3926..4441
                     /locus_tag="VIH70_02035"
     CDS             3926..4441
                     /locus_tag="VIH70_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY8115174.1"
                     /translation="MLKISQKLEYAMRAMIELSLRRDSGALVPAREIARAQQIPVRFL
                     EQQLGALNKAGLVESFRGAGGGCRLAKDPSEITVAQIADAIEGQLYPMFCLEPTDHTC
                     FADSRCGLQGFWGEVARAIDGVFQRTTVADLAERHQRRAGPTVFTSEQLLSGLNETVP
                     AEEAPQAPLPA"
     gene            complement(4477..4626)
                     /locus_tag="VIH70_02040"
     CDS             complement(4477..4626)
                     /locus_tag="VIH70_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEY8115175.1"
                     /translation="MYEGYCVKDREKVQFEGTVVELKNGRKAAQGICPKCGTKVMRIL
                     GKSDA"
     gene            4808..6769
                     /gene="acs"
                     /locus_tag="VIH70_02045"
     CDS             4808..6769
                     /gene="acs"
                     /locus_tag="VIH70_02045"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY8115176.1"
                     /translation="MSEADQNIAALLSEERLFEPPDTFVARAVVQDRSIYERADADHE
                     AFWAEQADRLGWVRRWDTVMAWDPPWVRWFEGGQLNAAYNCLDRHVEAGGGDKVAYYW
                     EGEPGEERAITYRDLLDDVCRFANALKGLGIRRGDRVAIYLGMVPELPVAMLACARIG
                     APHSVVFGGFSSEALRDRIIDAEARVLITADGGYRRGTVVPLKANAGEALRECPTIEH
                     VVTVRRTGSEHAFTEGRDHWYHDLVEGQSTECGPEPMDAEDPLYLLYTSGTTGKPKGI
                     VHTTGGYLTQVAATHRMIFDIHEDDVYWCAADIGWVTGHSYIVYGPLANHTTSVIYEG
                     APDWPDKDRLWQIIEKYRATILYTAPTAIRAFMRWGPEYPEKHDLSSLRLLGSVGEPI
                     NPEAWVWYWTYIGGERCPVVDTWWQTETGAILITPLPGITALKPGSATFPFPSIVADV
                     VDEKGSTVPLGGGGYLVLMRPWPSIARTIWGDPDRYVETYFSKYGPEIYVAGDGAKRD
                     EEGYFWLLGRIDDVMNVAGHRLSTYEIESALVDNSKVAEAAVVSRPDDVVGEAIVAFV
                     TLKSGFEGDDAVASELRDHVAHKIGKIARPHGIIFTTDLPKTRSGKIMRRLLRDVARG
                     RPLGDVTTLANAEIVEQIHEMVETGGTDE"
     gene            6825..7295
                     /locus_tag="VIH70_02050"
     CDS             6825..7295
                     /locus_tag="VIH70_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115177.1"
                     /translation="MAVAARALEPAGSSGCTISGTNGNDVLRGTASRDVICGRNGDDA
                     LVGLGGDDRIVGGRGNDRIDGGPGADDLNGGRGADLVTGGGSDDHVEGGPSRDAIRGD
                     SGSDIVQGGRGRDFCVATDDGIVGNDVANGGPGQDAYHADAGDTLVSVEAARDC"
     gene            7497..>8719
                     /gene="groL"
                     /locus_tag="VIH70_02055"
     CDS             7497..>8719
                     /gene="groL"
                     /locus_tag="VIH70_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133521.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8115178.1"
                     /translation="MAPKLISYDEDARRALERGMDQLANAVKITLGPKGRNVVLEKKW
                     GAPTITNDGVSIAKEIELEEPVEKIGAELVKEVAKKTDDVAGDGTTTATVLAQSIVKE
                     GLRNVAAGANPMAIKRGIERAVELAVESIKNQSKEVEQKEEIAHVGAISAADPEIGEQ
                     IAEAVDKVGKDGVITVEESNTFGMELELVEGMRFDKGYISAYFVTDPERMETVLDEPY
                     ILIANSKISAVKDLLPVLEKVMQGGKPLLIIAEDVEGEALATLVVNKMRGTFRSSAVK
                     APGFGDRRKAMLQDIAILTGATVVSEEVGLKLENATLDLLGTARKVMLTKDDTTIVEG
                     GGKEEDIKGRINQIKAEIDKTDSDYDREKLQERLARLAGGVAVIKVGAATEVELKEKK
                     HRIEDAVQSTKAAVE"
BASE COUNT         1431 a         2889 c         3117 g         1282 t
ORIGIN      
        1 tacgtcggtc tgtacgggct tgaggacggc gcgtcggagc tggtgaccgc gctcgtgccg
       61 cgtgcggagg cggccgccag cgaggtgccg gaggcctggg tcgaggacaa aggggtctcg
      121 gtcgcggtcc actaccggca ggcgccggat ccggcatcgg cgagagcggc gctgctggtt
      181 gcgctgcagc ctgtcgcaac cgaggccggt ctggagatca tccagggcaa gatggtggtc
      241 gagctcgtac cgccggagcg gccgttgaag ggtggcgcgg tcgaacgcct cgtcggcgaa
      301 cacgcgctcc aggcggtctt gtacgcgggc gacgatcacg ccgatctcga tgcgttcgcc
      361 gcgctcgacc ggctgtcggc ttcgggggag gtcggtgtga ccgttcgcgt cgccgtccgg
      421 ggaccggaga cgccgccggc gatgatcgag gcggccgacg tggtcgccga cgggccggag
      481 ggcgtcgtcg acctcctgcg gacgctctag cggcgccgcc gcttcgcgcg ctcgagatcc
      541 tcgagctgtc ccccgaccca ccgtgccggc ggattcgcga gcaccagccg ggagagcccc
      601 cgcgagcgac gggtgcgctc gtccacgggc atcgcgatcg cctgttcgag tgcgtcggcc
      661 atctcgtcca cgtcgaacgg gttcactggc agcgcgtacc gaccgagccg cccgtacgct
      721 cccgcgttgc gggaaagcac caggacgccg cgtcgccggt tcagcaacgg tccctccatc
      781 gcgaccaggt tcatcccgtc gatcaccggg ttcacgaaca gcacgtcgta caggccgtac
      841 gcggcgatcg cgccgatgta gtcgtccttc aggcgcagtt cgatcggcag ccagccgtcg
      901 cggccgaggg cgtcgttcac gcggcgggcc tcgtcaaggc aggcctccgt gtactcgcgg
      961 tactccggca attccatccg cgagggcgag agcagcgcga ggaaccggac ccgtccgatc
     1021 cacgacgggt tccgacgcag gaagagctcg tacgcctgga acccgcggac cacgttcttg
     1081 gtgagctcga gccggtccac gcgcaggatg aggcggttgt tgtcgcgcca cctgttgagc
     1141 gcgtgccgga ggctgcgcac gcgcggcgtc gccgcctctt cgcgcatcgc gcggccgtcc
     1201 acggagatcg ggtggatacg gaccgtcacc tcgcgaccct cggtcacgat gcgcgagcgg
     1261 gcgaggtcga ccctggcacc ggggaactga cgggcggaga gcaggaagtt ctcggcccac
     1321 gccggggagt ggaacccgag gacgtccgcc cccatcaggc ccccgaggac ctgatcgtgg
     1381 atccgcgccg gcagcatgcg gaagtacgtg ggacccgcga tcgaggtgtg cgagaaatgc
     1441 gcgatcagcg cgttcgggac cagctcgcgc aggatcctgg ggacgaggga caggtggtag
     1501 tcctggatca ggaacgcggt gtcacccccg agtgcatcgc cctcctcggc caaggccgtc
     1561 gcgaaccgtc gattgacgtc gacgtacgcg tcccacgcct gctcgacgtc cgatccccac
     1621 gacggcgacg tgaccgtgtc ccacaggtag tggtacgcga accacaacat gccgttcgag
     1681 acgcggttgt agtaaccgtc gtagatctcg ggcggaatgt cgaggtagcg gaggcggtac
     1741 cggacttcgc cctccgcgtg gtcgatcctg ccgcccttgg acgccgcgac catctcgcgg
     1801 tcgccctcgg acatcgcggc ggccacccaa acgccctccg cctcgatcag ggcgctagac
     1861 agggccgtga cgagaccccc ggacccgcga cgagcctgga acccgccgcc cttggcgcgt
     1921 tcgaacgtca gggggcctcg gttcgaggcg acgatgatcg gcgggctcat accgtgagcc
     1981 gagagatcag cctgagcgtg tcctcgatct cgcggaggca caggaagcgc gctcggacga
     2041 gctcccgtcc cgcctcgccc atcgccttgc cccgctccgg gtcccgcagg agctcgacga
     2101 cgtgctgggc ggtctcgccg atgccgttca cgaggaagcc ggtcaccccg tcctggatct
     2161 gcaggcggat gcccccaacg tccccgccca ccacgggctt ccacttccac atcccctcgg
     2221 cgacgacgag cccgaacccc tcgcgcaggg acttctggac gatcacgttg gacgcgcgct
     2281 ggaacgcgtt caccgcgctg tccccgacgc cgctcaggtt cgtgagcaga tggatgtcgg
     2341 gctctccggc ggcgtgctcg gtcgtgagat ccaggtaccg gatgccctcg ggatcgtcgt
     2401 gcgcgagcga cccgaccaac gccagctgag cgtccgggac ctcgcgacgg atcaggcgga
     2461 acgcgtcgat gacgcccagt ggatccttcc aggggtcgaa gcgtgagacc tgggtcacga
     2521 gaggtcgatc ggggtccacg ccgaacggcg ccaggacgcc ctgccaggtg gactccatca
     2581 tcggcgcgtt cttcgggctg agcgggtcga tcgatggtgg gatgaacgcc cggaccggtc
     2641 cctgcacccc gggctggacg tactcctccg ccgtgaagat ggccgcgtcg tatcggttca
     2701 cgatcggttc gaagaagtcc cataccggac ggtacggggc cgacaggtcg atgtggcagc
     2761 gccagatcca cttgcccttg cgacgcgagc cctcctcgag gacctgcagg agcgcggcgg
     2821 gctgcgggtc gtggaccagc acgacgtcga agtcgtcggg gaactcggcc gcgttcgcgt
     2881 gcatccgctc ccaatacgtc tcgcgcatgt cctcgtcgag gtgcgtctcg gcgccctgca
     2941 gggcgttgtg gacggccttg gtgacgttga agaatcggtc cgaaccctcg atgacgaccc
     3001 acgtcgtgtc gacgccgagg tcgttcatca gcgggacctg cgtctggagc agctcggaga
     3061 ctccaccgcc gtaggcggtg ctgctgacgt tcaggaccct gaccccctgg agctgccgcg
     3121 ccgcctctcg gaggctatcg agggcctcct gccccgccgc ctccgcgtac tccgacacgc
     3181 gcttgggttg gaccgggacg atcgggagca tgcttcgagt ctacgtgggc caggcggggg
     3241 tggccgacgg ctcaggcacc gagctgcgcg agctctcgct gccggcgatc cgagcgctcg
     3301 acggcagacg tctctcgggc gcgctcggcg atgccgacga ggtcgaccgg ggagaagggt
     3361 ttcgtcaggt actcggtgca cccggcgcgc cacccggccc ggcgatcctc gagctgcgtc
     3421 ttggccgtga gcaggatcac ggggaggtcc cgggtctgtt cgtcgttcgc caacgtcccc
     3481 aggaccgaga tgccgtccaa cctcgggagc atgacgtcga gcacgaccac gtcgggctgc
     3541 tcgtgcaggg ccagctcgag gcccgcctcg ccgttggtcg cctcgatgac ctcgtgcccg
     3601 gcgagctcga ggttcaatct gcagagcatc aggacgtccg gctcgtcgtc gatcaccagt
     3661 acccgcacgt cgtctccatc ctcggaccgc ttgcttcacg gggtatcggc cgagacgggg
     3721 gcacgcctta tggtccaacc ggaccatctc tcgggaacgc gaccggtttt cggcgcgcgt
     3781 gccaggcccg gcggggtgcg tgccaggcac gggagggctc gttgacaccc cctgaggccc
     3841 ttcctacatt gccccgagga acctaggcca ggggctcagc agggatgagc accgatgaac
     3901 ggcggataag gcggataagc gggacatgct gaagatctcg cagaagctcg agtacgccat
     3961 gcgggcgatg atcgagctgt cgctgcgccg tgacagcgga gccctcgtgc ccgcacgcga
     4021 gatcgctcgg gcgcagcaga tcccggttcg gttcctcgaa cagcagcttg gcgcgctcaa
     4081 caaggcgggc ctggtggaga gcttcagggg ggccggcggc gggtgccgcc tggccaagga
     4141 tccgtcggag atcaccgtgg cccagatcgc ggacgcgatc gaggggcagc tctacccgat
     4201 gttctgcctg gagccgaccg accacacgtg cttcgcggac tcgcggtgcg ggctgcaggg
     4261 gttctggggc gaggtggcca gggcgatcga cggcgtcttc cagcgcacga ccgtcgccga
     4321 tctggccgaa cgccaccagc gtagggccgg cccgacggtc ttcacctcgg agcaactcct
     4381 ctcggggttg aacgagacgg tgcccgccga ggaggctccg caggcaccgc tgcctgcctg
     4441 aacgggaccg gggggcccgg cccctgggga gagcctctac gcgtcgctct tgcccaggat
     4501 ccgcatcacc ttcgtcccgc acttgggaca gatgccctga gcggccttcc ggccgttctt
     4561 gagctcgacg accgtcccct cgaactggac cttctcgcgg tccttcacgc agtacccctc
     4621 gtacatggcc acgtgggcct ccttcccccc gcagggttcg aggccgcctc cattggcggc
     4681 ctccaggcac gatgctgggg cctgccaggc acggatgcaa gcacatcgag acccattcca
     4741 ggatgccccc ggtggctgcg ggccgtacga tgcgtgactg tcgggccgag agagggggga
     4801 agcacccatg tcggaggccg atcagaacat cgcagcgctg ctctcggagg aacggttgtt
     4861 cgagcctccg gacacgttcg tggcccgggc ggtggtccag gaccggtcga tctacgagcg
     4921 tgccgacgcc gaccacgagg cgttctgggc ggagcaagcg gaccggctcg gctgggtcag
     4981 gcgctgggac accgtgatgg cctgggatcc tccctgggtc aggtggttcg agggggggca
     5041 gctgaacgcc gcctacaact gcctcgaccg acacgtcgag gccggcggcg gcgacaaggt
     5101 cgcctactac tgggagggcg agccgggaga ggagcgggcc atcacctacc gggacctcct
     5161 cgacgacgtc tgccggttcg cgaacgccct caaggggctg ggcatccgca ggggcgatcg
     5221 ggtcgcgatc tacctgggca tggtccccga gctcccggtc gcgatgctcg cctgcgcgcg
     5281 gatcggggcc ccgcactcgg tggtgttcgg cgggttctcg tccgaggcgc tccgcgatcg
     5341 catcatcgac gcggaggcca gggtcctgat caccgcggac ggcggctacc gccgcgggac
     5401 cgtcgtcccg ctcaaggcga acgcgggcga ggccctgagg gagtgtccga cgatcgagca
     5461 cgtcgtcacg gtccgtcgga ccggcagcga gcacgcgttc acggagggac gggaccactg
     5521 gtaccacgac ctggtcgagg ggcagtcgac ggagtgcggg cccgagccga tggacgcgga
     5581 ggacccgctg tacctgcttt acacgagcgg caccaccggg aagcccaaag gcatcgtgca
     5641 tacgaccggc gggtatctca cgcaggtggc ggcgacgcac cggatgatct tcgacatcca
     5701 cgaggacgac gtctactggt gcgccgcgga catcggatgg gtcacggggc attcgtacat
     5761 cgtgtacggc ccgctggcca accacaccac gagcgtgatc tacgagggcg cgccggactg
     5821 gccggacaag gatcgcctct ggcagatcat cgagaaatac cgtgcgacga tcctgtacac
     5881 agcgccgacc gcgatccggg cgttcatgcg gtggggaccc gagtaccccg agaagcacga
     5941 cctgtcctcg ttgcgtctgc tcgggtcggt cggtgagccg atcaacccgg aggcgtgggt
     6001 ctggtactgg acgtacatcg gcggcgaacg ctgtccggtg gtggacacct ggtggcagac
     6061 ggagacgggg gcgatcctga tcacgcccct gcccgggatc acggcgctca agccggggtc
     6121 ggcgaccttc ccgttcccga gcatcgtcgc cgacgtcgtc gacgagaagg gttcgacggt
     6181 gccgctcggg ggagggggat acctcgtgct catgcgcccg tggccgtcca tcgcgaggac
     6241 gatctggggc gatcccgatc gctacgtcga gacctacttc tcgaagtacg ggccagagat
     6301 ctacgtcgcc ggcgacggcg cgaagcggga cgaggagggg tacttctggc tgctcggccg
     6361 gatcgacgac gtcatgaacg tcgcggggca ccggctgtcg acgtacgaga tcgagagcgc
     6421 cctcgtggac aactccaagg tcgccgaggc cgcggtggtt tcacgacccg acgacgtggt
     6481 gggcgaggcg atcgtcgcgt tcgtcacgct gaagagcggg ttcgaggggg acgatgcggt
     6541 cgccagcgag ctccgagatc acgtcgcgca caagatcggc aagatcgcac ggccccacgg
     6601 gatcatcttc acgaccgatc tcccgaagac gcgttcgggc aagatcatgc gcaggctcct
     6661 ccgcgatgtc gcccgcggtc gcccgctcgg cgacgtcacc acgctggcga acgccgagat
     6721 cgtcgagcag atccacgaga tggtggagac cggcggcacc gacgagtgac cggtgcggtc
     6781 gcacggcggc cgtcgtcggc atcgtcgcgt cgctgatcgt tccggtggcc gtggccgcgc
     6841 gggcgctcga gcccgccggc tcgtccgggt gcaccatctc gggcacgaac gggaacgacg
     6901 tcctgcgggg gaccgcgagc cgggacgtga tctgcggtcg caacggggac gacgcgctcg
     6961 tggggctggg cggcgacgat cggatcgtcg ggggccgggg caacgatcgg atcgacgggg
     7021 gacccggcgc cgacgacctt aacggggggc ggggggccga tctcgtcacg gggggaggct
     7081 ccgacgacca cgtcgagggc ggccccagcc gcgacgcgat tcggggcgac tcggggtcgg
     7141 acatcgtgca gggcggtcgg ggtcgggact tctgcgtggc caccgatgac ggcatcgtgg
     7201 gcaacgacgt cgcgaacggc ggaccgggcc aggacgcgta ccacgccgac gcaggggaca
     7261 ccctcgtgag cgtcgaggcg gctcgggatt gctagctcac gccggaggtc gtctggcgcg
     7321 cctcgatcga agcgtgccca ggtagagggt cggcgagtcc tcgtgcccgg agtccgcctt
     7381 gccattcgac ggcccgcctg ggtaccattc gttagcactc ggttgccgag agtgctagca
     7441 gtcatccagg gtatctgcgc agacagcggc gcggaaccga gcacggagga ggagcgatgg
     7501 caccgaagct catctcgtac gacgaggatg cacggcgggc gctcgagcgt ggcatggacc
     7561 agctcgcgaa cgccgtcaag atcacgctcg gccccaaggg ccgcaacgtg gtcctggaga
     7621 agaaatgggg cgccccaacg atcaccaacg acggcgtctc gatcgccaag gagatcgagc
     7681 tcgaggagcc ggtcgaaaag atcggcgcgg agctcgtgaa ggaggtcgcc aagaagaccg
     7741 acgacgtcgc gggtgacggc acgaccaccg cgacggtgct cgcacagtcg atcgtgaagg
     7801 agggcctgcg caacgtcgcg gccggtgcga acccgatggc gatcaagcgc gggatcgaac
     7861 gcgccgtcga gctcgccgtg gagagcatca agaaccagtc caaggaggtc gagcagaagg
     7921 aggagatcgc tcacgtcggc gccatctccg ccgccgaccc cgagatcggc gagcagatcg
     7981 ccgaggccgt cgacaaggtc ggcaaggacg gcgtgatcac ggtcgaggag tccaacacct
     8041 tcggcatgga gctcgagctc gtcgagggca tgcggttcga caagggctac atctcggcgt
     8101 acttcgtgac cgaccccgag cgcatggaga cggttctcga cgagccgtac atcctgatcg
     8161 cgaactccaa gatctcggcg gtcaaggacc tgctcccggt cctcgagaag gtcatgcagg
     8221 gcggcaagcc gctgctgatc atcgccgagg acgtggaggg ggaggcgctc gcgacgctcg
     8281 tcgtgaacaa gatgcgcggc accttccgct cgagtgcggt caaggccccc gggttcggcg
     8341 accggcgcaa ggcgatgctc caggacatcg cgatcctcac cggcgccacc gtggtctccg
     8401 aggaggtcgg cctgaagctc gagaacgcga ccctggacct gctgggcacc gcccgtaagg
     8461 tcatgctgac gaaggacgac accacgatcg tggagggcgg cggcaaggag gaggacatca
     8521 agggccggat caaccagatc aaggccgaga tcgacaagac cgactccgac tacgaccgag
     8581 agaagctgca ggagcgcctg gcgcgcctcg ccgggggcgt cgcggtcatc aaggtcggcg
     8641 ccgcgaccga ggtcgagctc aaggagaaga agcaccgtat cgaggacgcc gtgcagtcca
     8701 cgaaggccgc cgtcgaaga
//
LOCUS       DATBRK010000066         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1594814, whole genome shotgun sequence.
ACCESSION   DATBRK010000066 DATBRK010000000
VERSION     DATBRK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594814"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VIH70_02060"
     CDS             complement(<1..263)
                     /locus_tag="VIH70_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115179.1"
                     /translation="MPTKVVVCDTPDGLAQLQYVLIMSGAAMEIETVTDGMRAVEVSA
                     RIHPEFVVAGVDAESLSGAELVRRLRAVSPETKVICLADASPLV"
     gene            complement(351..1220)
                     /locus_tag="VIH70_02065"
     CDS             complement(351..1220)
                     /locus_tag="VIH70_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8115180.1"
                     /translation="MLEGLANRLLGGRATVRRTRCRGVLLVRGHPGAPVRGGFVCGLV
                     HERSFLRRRSASILRDDPREDPLTAERQPKPREEGPIRILIVDDHPVTREGLHAALDL
                     ANDVVVVGESDSGEDAVVKVGELTPDIVFMDVRMPGMNGIEATKAIRQASPSTRVILF
                     TVDESRASISEAIQAGVSGYLLKDASADELVNAARLALEGKAVIHPQLTRAFIEEVQI
                     AEKRVEAPPLSRREKEILQKVAYGATTKEVAHDLGISPHTVKTHLERIFEKLGANDRA
                     QAVAIAIRSGLVE"
     gene            1131..1484
                     /locus_tag="VIH70_02070"
     CDS             1131..1484
                     /locus_tag="VIH70_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEY8115181.1"
                     /translation="MTADEQHAPAPGASNGGPATEEAVREALKHVVDPELGVNIVDLG
                     LVYDVEISEEGAISIEYTLTTMGCPIGPLIEHQMQSFLENVPGVTSVEAEMVLRPPWT
                     PEMMSEEAKAALGYF"
     gene            1785..2630
                     /locus_tag="VIH70_02075"
     CDS             1785..2630
                     /locus_tag="VIH70_02075"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY8115182.1"
                     /translation="MAGYAKPEALVETDWLEEHVKDPGIRVIEVDEDTEAYEKGHIDG
                     AVGWNWTTDLHAEVGRDYVDQAELSELLSAAGVDPDTTVILYGGNNNWFAAYAYWVLK
                     LRGFDNVKLLNGGRKKWELESRQLAQDPPSVTPSGFSISGPERGEIRALRDEVLGKVG
                     GTTGFVDVRAPEEFRGEKLAPDHLPQEQAQVPGHIAGAANIPWAKAANEDGTFKSADE
                     LRALYEGEGITGDREIIAYCRIGERSSHTWFALQELLGYPNVKNYDGSWTEYGSLVGA
                     PVEKG"
     gene            complement(2676..>2996)
                     /locus_tag="VIH70_02080"
     CDS             complement(2676..>2996)
                     /locus_tag="VIH70_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115183.1"
                     /translation="RAYGLMQGKVMQVLSPRSALPWIRAELSPETRQRGLKGGSFMQM
                     PGTGGVVRFTDGLIRFAHRNKHVADSPANDLILRVVASLVGRARDDIASAGDAGPDPQ
                     PNPL"
BASE COUNT          487 a          982 c         1058 g          469 t
ORIGIN      
        1 accagcggcg aggcgtccgc caagcagatc accttggtct cggggctgac ggcccgcagg
       61 cggcggacga gctcggcccc ggacaggctc tcggcatcga cgccggcgac gacgaactcc
      121 gggtggatgc gcgcggagac ctcgacggcc cgcatgccgt cggtcacggt ctcgatctcc
      181 atcgcggcac ccgacatgat caggacgtac tggagctgtg cgagaccgtc gggcgtgtca
      241 cataccacga ccttcgtggg catcgtgttc ctggtatcgg cggatgccgg aaggggttga
      301 gggtggggga cgcgcgggct agggcggagg gcctaggcgc cgcgggcgag ctactcgacc
      361 agaccgctgc ggatcgcgat cgccacggcc tgggcccggt cgttcgcccc gagcttctcg
      421 aagatccgct cgagatgggt cttcaccgtg tgcggtgaga tccccagatc gtgcgcgacc
      481 tccttcgtcg tggcgccgta cgccaccttt tggaggatct ccttctcccg gcgcgagagc
      541 ggaggcgcct cgacgcgctt ctcggcgatc tgcacctctt cgatgaacgc gcgggtgagc
      601 tgtgggtgga tcacggcctt gccctccagg gcgaggcggg ccgcgttcac gagctcgtcc
      661 gcggaggcgt ccttcagcag atagcccgac acaccggcct ggatcgcctc ggagatcgag
      721 gctcgcgact cgtcgaccgt gaacaggatc accctggtgc tcggcgatgc ctgcctgatc
      781 gccttcgtcg cctcgatgcc gttcatgccc ggcatccgca cgtccatgaa gacgatgtcc
      841 ggagtgagct cgccgacctt cacgacggcg tcctccccgc tgtcggactc gccgacgacg
      901 acgacgtcgt tcgccaggtc gagggccgcg tggagcccct ccctcgtgac cggatggtcg
      961 tcgacgatca ggatgcggat ggggccctcc tcgcgtggct tcggttggcg ctcggcggtc
     1021 agcgggtcct ccctcggatc gtcccttagg atagaggcag accttctccg aaggaacgat
     1081 ctctcatgga cgagtccaca cacgaaccca ccacggacgg gggcaccggg gtgaccgcgg
     1141 acgagcagca cgccccggca ccgggtgcgt cgaacggtgg cccggccacc gaggaggcgg
     1201 ttcgcgaggc cctcaagcac gtcgtggacc cggagctggg cgtcaacatc gtcgacctcg
     1261 gtctggtcta cgacgtggag atcagcgagg agggcgcgat ctccatcgag tacaccctga
     1321 ccacgatggg gtgcccgatc ggaccgctga tcgagcatca gatgcagtcc ttcctggaga
     1381 acgtgcccgg cgtcacctcg gtggaggcgg agatggtcct ccgccccccg tggacgccgg
     1441 agatgatgtc cgaggaggcc aaggccgctc tcgggtactt ctagcgacgg cgccggccgc
     1501 gagcacgcgc atcccgcgaa cccccattga cactcgcaac gaccggctgt tgacttgcac
     1561 ctgcaattcc cgagtagggg ggtcggattc gtggttcggt cgagatgtgc aggtctggtg
     1621 gcggcgatga tccccgcccg actctgcggc tgtaccgagc ccgggcgcct taccggcgcc
     1681 ccagaggtgc cgcgctagcc gaactcccag gaccgatccg aggggccgcg cggcggcccc
     1741 ttttcgcgtt tgtcatggga gttccgatcc ggaaggagga agagatggcg ggatacgcca
     1801 aacccgaagc gctcgtcgag accgactggc tcgaggagca cgtgaaagac cctggcatcc
     1861 gagtcatcga ggtggacgag gacaccgagg catacgagaa gggtcacatc gatggcgcgg
     1921 tcggttggaa ctggacgacc gacctgcacg ccgaggtggg gcgcgactac gtcgaccagg
     1981 ccgagctctc cgagctcctc tcggccgcgg gggtcgaccc cgacaccacc gtgatcctgt
     2041 acggagggaa caacaactgg ttcgcggcgt acgcgtactg ggtgctgaag cttcgcgggt
     2101 tcgacaacgt gaagctcctg aacggcggtc gcaagaagtg ggagctcgag agccgccagc
     2161 tcgcgcagga ccccccctcg gtgaccccct ccggcttctc gatctcgggc cccgagcgcg
     2221 gcgagatccg ggcgcttcgt gacgaggtcc tcggcaaggt ggggggtacg accgggttcg
     2281 tcgacgtccg cgcgccggag gagttccggg gcgagaagct ggctcccgat caccttccgc
     2341 aggagcaggc gcaggtgccg gggcacatcg ccggcgcggc taacatcccg tgggccaagg
     2401 cggcgaacga ggacgggacg ttcaagagcg cggacgagct gagggccctg tacgagggcg
     2461 agggcatcac cggcgaccgc gagatcatcg cgtactgccg gatcggcgag cgctcgagcc
     2521 acacgtggtt cgccctgcag gagttgctcg gctacccgaa cgtcaagaac tacgacggct
     2581 catggaccga gtacggctcg ctcgtgggcg caccggtcga gaagggatga ccacgggaca
     2641 gtgacgtcgg tgtgggaggc gaaccaggtt cgtgctcaca gggggttcgg ctgaggatcg
     2701 gggcctgcgt ctcctgccga cgcgatgtcg tctcgggccc tgccgacgag cgaggcgacg
     2761 accctcagga tgaggtcgtt ggccggggaa tccgcgacgt gcttgttgcg gtgggcgaac
     2821 cggatcagac cgtccgtgaa ccggaccacg cccccggtcc cgggcatctg catgaagctc
     2881 ccgcccttca gtccccgctg gcgggtctcg ggtgagagct cggcgcggat ccacgggagg
     2941 gccgatctgg ggctgagcac ctgcatcacc ttgccttgca tcagcccata cgcgcg
//
LOCUS       DATBRK010000067         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1163791, whole genome shotgun sequence.
ACCESSION   DATBRK010000067 DATBRK010000000
VERSION     DATBRK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163791"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VIH70_02085"
     CDS             <1..975
                     /locus_tag="VIH70_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8115184.1"
                     /translation="GIPVARLMEGEMAKLLHLEEALHRRVVGQDEAVSAVANAIRRSR
                     AGLSDPTRPIGSFLFLGPTGVGKTELARALAEYLFDDERAMVRIDMSEYQERHTVSRL
                     VGAPPGYIGFEEGGQLTEAVRRRPYSVLLLDEIEKAHTDVFNVLLQLLEDGRLTDGHG
                     RTVDFRNAVVIMTSNLGGAIFRETEISADKRREAVLEDVQGFFRPEFVNRIDEIVVFE
                     PLTPEQIGAIVDIQIRLLAERLAERSLGLELTDAARDLIAARGYDPAFGARPLKRLIQ
                     REIQDPLAVKLLSGEIGEGDTVLVDADGESLTLRAVSGLGPAWAESAD"
     gene            1038..1442
                     /locus_tag="VIH70_02090"
     CDS             1038..1442
                     /locus_tag="VIH70_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY8115185.1"
                     /translation="MTDPGANADRPRLLRRSRDDRVIGGVCGGLGRYLGVDPVLLRIA
                     MVILAIAGGGGILIYLVAWVLIPREREGEDLGSARPEGADTTRLLVGGALIAIGTILL
                     LNLSVPWLGKYFWPLALIAVGVAVVIQASSRR"
BASE COUNT          225 a          565 c          593 g          279 t
ORIGIN      
        1 ggcatcccgg tcgcccgcct gatggagggc gagatggcca agctcctgca cctggaggag
       61 gcgctccatc gccgcgtcgt cgggcaggac gaggccgtct ccgccgtcgc gaacgcgatc
      121 cggcgctccc gagccgggct ctcggatccg acgcggccga tcggctcgtt cctgttcctg
      181 ggcccgaccg gcgtgggcaa gaccgagctt gctcgcgcgc tcgccgagta cctgttcgac
      241 gacgagcgcg ccatggtccg gatcgacatg agcgagtacc aggagcgtca caccgtgtcg
      301 cgcctggtcg gcgcgccgcc cgggtatatc gggttcgagg aaggcggcca gctcaccgag
      361 gccgtccgcc ggcgtcccta ttccgtcctg ctgctcgacg agatcgagaa ggcgcacacc
      421 gacgtgttca acgtcctgtt gcagctgctc gaggacgggc gtctcaccga cgggcacggg
      481 cgaacggtcg acttccgcaa cgcggtggtg atcatgacct ccaacctcgg aggcgccatc
      541 ttccgggaga ccgagatctc tgccgacaag cgccgcgagg cggtcctgga ggacgtgcag
      601 ggcttcttcc gtccggagtt cgtgaacagg atcgacgaga tcgtcgtgtt cgagccgctc
      661 acgcccgaac agatcggtgc gatcgtggac atccagatcc gcctgctcgc ggagcgcctc
      721 gccgagcggt cgctgggcct cgagctcacc gacgcggccc gcgatctcat cgcggcccgt
      781 ggttacgatc ccgcgttcgg ggcgcggccg ctgaagcggc tcatccagcg cgagatccag
      841 gacccgctcg ccgtgaagct gctctccggc gagatcggcg agggagacac ggtgctcgtg
      901 gacgccgatg gggagtccct gacgttgcgg gccgtctccg gtctcggccc cgcctgggcc
      961 gagtccgcgg attgatccgg cctcgacccg gggcgaccgg ttcggggaag ctcggcgggg
     1021 cgtcgtagcg cgaggccgtg accgacccgg gcgcgaacgc cgatcgacct cgactcctgc
     1081 ggcgcagccg ggacgaccgc gtcatcggcg gcgtctgcgg tgggctcggt cggtacctcg
     1141 gggtggatcc ggtcctgctg aggatcgcga tggtgatcct ggcgatcgcg ggtggcggcg
     1201 ggatcttgat ctacctcgtc gcgtgggtgc tgatccctcg cgaacgcgag ggtgaggatc
     1261 tcggctcggc cagaccggag ggcgccgaca cgacgaggct gctcgtcggc ggcgcgctga
     1321 tcgcgatcgg gacgatcctt ctgctcaacc tgtccgtccc ttggctcggc aagtacttct
     1381 ggcccctcgc gctgatcgcg gtcggcgtgg cggtcgtgat ccaggcctcg tctcggcgat
     1441 gattcggtac ctccgccgcc acctgcgggt cccggcggcc cgatggcggg acctcggcga
     1501 gcgcctcggg tcgtcgccat gcgagcgagg ggctaggctt cccgtcgacc tctgcagaag
     1561 ggaacgaccg tgggttggac catcgcgatc atcggcggga tcgtcctgct gatcctgatc
     1621 ttcggcgtcg tctcgtacaa ccgattcgtc tcccagcgtc ag
//
LOCUS       DATBRK010000068        12890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1017191, whole genome shotgun sequence.
ACCESSION   DATBRK010000068 DATBRK010000000
VERSION     DATBRK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12890
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017191"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VIH70_02095"
     CDS             <1..289
                     /locus_tag="VIH70_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEY8115186.1"
                     /translation="NGSDALLGSDGGDRLLGGRGDDRLRGGVGADVLDDSLRVAQGVS
                     PDTGADLLSGGPGDDRLRGGDEVRANDTLHGGEGHDACSADPGDRISGCRP"
     gene            complement(367..648)
                     /locus_tag="VIH70_02100"
     CDS             complement(367..648)
                     /locus_tag="VIH70_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115187.1"
                     /translation="MLQVTDAAVSVFKKVLEQEERGGGGIRLVPNPQPDGRTSVGVEP
                     IREAGPNDEPTDARGLTVVVARDLAPELEDAVLDAKETATGADLLVRPQ"
     gene            complement(903..1346)
                     /locus_tag="VIH70_02105"
     CDS             complement(903..1346)
                     /locus_tag="VIH70_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115188.1"
                     /translation="MATILVNERPCGIGGDPPRFGHPAELAEGHRARSLDERGFGSRE
                     RLWSSLLGGCEHMDGRFGDLTFPERAGHLGHRFERTSATDAALGCRARQPLPLRQPRR
                     RRQVAVDRVDLSTSELRQAATALELEDASRALDLIQVTGERLVRQ"
     gene            complement(1554..1949)
                     /locus_tag="VIH70_02110"
     CDS             complement(1554..1949)
                     /locus_tag="VIH70_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115189.1"
                     /translation="MDRSVMWPAEQHEVAERRLATVGPVANVMGVAPAGWPSTAWKPT
                     SAVPNGDRASEAGRHDLGATAELERLGARPRDHSSDIGVAGEAASGFGRDLAGVLQLR
                     ALSGTLDQRLQVDRDHDVRALAADDRALA"
     gene            complement(2059..3033)
                     /locus_tag="VIH70_02115"
     CDS             complement(2059..3033)
                     /locus_tag="VIH70_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8115190.1"
                     /translation="MSPVTAVETALLEVEDLVVEFGPVRAVDGVSFRLPAGPYGLALV
                     GESGSGKTTIARALMRLVSATSGAIRLDGSEVVGSSRALRAYRRDVQIVFQDPTTSLD
                     PRAKIGSTIAEPLRAHGIVPRAEVHERIASLLAEVGLDPEMAGRYPHQLSGGQRQRVA
                     IARALSVEPRVLVLDEPTSALDVTVQARILELIAKLRRERGLAYVLISHNLAVVEQLC
                     EETAVLYLGRIVEHGATEQILARPAHPYTLALRSAVPEIALAARRSRIVLPGTVPNPA
                     NPPPGCPFHPRCPYAIDRCRTEVPQLRVVDAGRVVACHRAEEILVGEI"
     gene            complement(3030..3812)
                     /locus_tag="VIH70_02120"
     CDS             complement(3030..3812)
                     /locus_tag="VIH70_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8115191.1"
                     /translation="MTESRLSLQDLRIAFGPRMVVDLERLELGPGEILGLAGESGSGK
                     SITALAMLGLASTLGARVEGSVRLDGEELVGAPERRLREIRGSRISMIFQSPVSSLDP
                     LLQVGELFERTLKLHGVGRREASELASNAFREVLLPPDLLRRYPHELSGGQAQRIAIA
                     MALALRSEVLLADEPTSALDVTVQAEILDLLRRVREERGMSVLFISHDLAVIAELCDR
                     VAVMELGRIVEEGPTARVLGEPADQYTRRLVAAVPKIGAGTA"
     gene            complement(3809..4723)
                     /locus_tag="VIH70_02125"
     CDS             complement(3809..4723)
                     /locus_tag="VIH70_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8115192.1"
                     /translation="MSRVPSYPDVESREEVLAPPGRLRSVPGVIRRNPLLAVGAAMCL
                     AIFLVAVLAPLVATYPEDAGSAVHPLQTLEPPSAQHYFGTDQVGRDVFSRVVYGARIS
                     PLIAVLVLAISVLIGVPLGIAAGYFGGWIDEVISRVTDVFLAFPALLLALAFAAVMQP
                     SITNTVIAISVTWWPWYTRIVRGQAASVTGRPFVESAKATGVPHRTILLRHVLPNSIT
                     PIIVQVSLDVGGVILTASALSFLGLGAQDPTPDWGLMVSQGQAFFTTQWWLVTFPGAA
                     IFLTAVAFNLLGDGLRDVLDPRRVLATR"
     gene            complement(4723..5739)
                     /locus_tag="VIH70_02130"
     CDS             complement(4723..5739)
                     /locus_tag="VIH70_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8115193.1"
                     /translation="MTRFVVRRLALAALVVLGVVVLTFVIARVVPGDPAATWAGPRAR
                     PEQIEKAREELGLDRPIPVQIAEYVGGVASGDWGVSLRTRRPVLGDIGRRAPASIELV
                     TFATLLALAVGVPLGLVAARWKGKVPDIGSRLVAVVGVSMPSFWLAIVLQLVFFQWLH
                     LLPVAGTYDPNLDYTSPLHSITNMPIVDALLTGNWAVFGSSLRHIILPAIVIAAYPAG
                     VITRMVRASVLDTIGEDHVRMVRALGFPERSVFGRFALRHAWSPVVALLALVFAYALV
                     NTFLVEAIFNRPGLGSYAADAIGALDTPAILGVTLFVAIVYVIGNLLVDIVQAVIDPR
                     IKVS"
     gene            complement(5736..7343)
                     /locus_tag="VIH70_02135"
     CDS             complement(5736..7343)
                     /locus_tag="VIH70_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8115194.1"
                     /translation="MRSARFRWVVVLAAFAVAAAACTEDEGGGSTTGGGTNAEPDTFT
                     YSVNSEVMIGWDPATGYSNEVIALHNIYETLTRYNADTQEVEPLLADSWESSKDGLTW
                     TFTLHPGVTFHTGNPLTAEAAKAAIERTMKLGEGAAYIWGAVKKIDAPDDTTLVFHLS
                     YPSPLDLVSSGTYSAYIYDTTASGGQDLGDWFADGNDAGTGPYMVSEYNPGDEIELRL
                     EAYPDYWGGWDDADHYERYVFRVTPEANTAAQLLRAGEVSMVQRMSPQLWASFEGQDG
                     FETESAPSWQTLLGMLNTADGPLADPLVRDAVTKAIDYDGMIAALEGAADPLSGVVPP
                     GLWGHTDGLEYQQNVDEARTMLEQAGFGPSGDPIDLSLTYLKGDADEELVSTLMKSNL
                     ADLNINVDVRGLQWQTQWDQGKSANPADRQDIFIFYWWPDYPDPVSWFYNLFRTEKEP
                     FFNLAYYSNPALDSEIDRASVVAATDRDRATQMYGDMQRTLFEDAPAIPLYTQVYQRA
                     MLDSVDGFVDNPAYPNVVFGYELTPAA"
     gene            complement(7384..8925)
                     /locus_tag="VIH70_02140"
     CDS             complement(7384..8925)
                     /locus_tag="VIH70_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY8115195.1"
                     /translation="MPSPDLRLGVDVGGTNTDAVVLDAEDRLLAKAKVPTTPDVTKGI
                     EAALAGVVEQVDRARITHAMFGTTHATNAVLERRGLRRVGVLRLGGPATQAIRPLFGW
                     PADLREAVSAGERIVHGGIEFDGRELSPLDAEAVARFAGEVAGRADAIAIASVFAPVS
                     DEHERAAEEIVRKELGDLPISLSSQIGSLGLLERENATVLNASLVDVARGVTSAIAGA
                     LERNGLRPVTFFAQNDGTLMDLEFAIRFPVLTIGSGPANSIRGAAYLTGRTDAIVVDV
                     GGTSTDVGVLVRGFPRESSFGVEIGGIRTNFRMPDLVTIALGGGTIVTEDALGPESVG
                     YRILEEALVFGGSTPTLTDAGVAAQRFALGDASRLARHGPLLAAAIARSDAALADAID
                     RVKTSREPVTLVAVGGGAPLVPDDLTGTSEVLRPDHSDVANAIGAAIASVSGEVDRVF
                     HPGVAGREAAIAEASQEAIERAVSAGAARESVEVVEIEEVPLAYLTDPAVRIRVKAGG
                     PLAWG"
     gene            complement(8912..9988)
                     /locus_tag="VIH70_02145"
     CDS             complement(8912..9988)
                     /locus_tag="VIH70_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HEY8115196.1"
                     /translation="MQLDASSLPSLARGCAILGAGGGGDTEIGLLASLQAVEDHGPVP
                     LVDLEELPDEALLMPCGGVGAPTVSIEKIESGDEGGRLREMVETLWNRPVVALMSAEI
                     GGSNGIIPVVWAARIGLPVVDADGMGRAFPEIPQVTMEIAGISPNPAVMTDERGNVIV
                     FRATDGDWMERMERAAAVEFGGMSWSSEYQLTVGQAKAGAVVNHAVSLAIRLGSVVAE
                     AEHEPVEALAHEIHAARLIVGKIVDVERRTTAGFVRGSATIEGLDADAGRVLRLEIQN
                     ENLVALEGERALAMVPDIITVLDSQSANAISTERLRYGQRVTVIAFPCDPIWRTARGL
                     ELAGPRHFGYDLDYAPVEDLHALA"
     gene            complement(10205..11344)
                     /locus_tag="VIH70_02150"
     CDS             complement(10205..11344)
                     /locus_tag="VIH70_02150"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY8115197.1"
                     /translation="MLDLLRPAPLIDGHNDLLWELRQGRDPADPTLMTDLPRMAAGGV
                     GGQFWSVYVPSDLPADRAVTMTFEQIDALLDLVRTNPDRLELARSADDVERIAAAGRV
                     ASMIGVEGGHAIGGSLGTLRVLAEFSMGYLTLTHNDDTRWADSATGEHSHGGLTRFGE
                     EVVREVNRCGWLVDLSHTSDDTMRQAIEVSAAPVIFSHSSARALCDVPRNVPDDVLEL
                     VGRTGGVVMVTFVPSFVAAEGAEFNLVGLAEARRVRAEHPDDPDAVRKAMDAWFEMNP
                     GPTATVSDVANHIDHVREVAGIDHVGIGSDFDGAPTMPEGLEDVSRYPVLLEELASRG
                     YGEEDLMKIAGGSVLRVMREGERTRTRLRGERSPSKATIEELDTA"
     gene            complement(11601..>12890)
                     /locus_tag="VIH70_02155"
     CDS             complement(11601..>12890)
                     /locus_tag="VIH70_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY8115198.1"
                     /translation="EVGGKDPGSWTADSVEIFQEGLQLPFVRVVRAGQLDRDLVAMIG
                     ANSRLPDMTIGDLTAFAACLEVAERRLLELCQRYGVEDVTSAMAAAHERSEALSRAAV
                     GRLPRGVFEAEDFIDEDGLGNGPFPIRVRVEIDGERVVADFTGTHPQVPGPVNCTRSG
                     LVSGIRTVFKAITDPNEPGSDAWFRPFEVVCPLGTIFTAQRPAPVALYFEATEAATDL
                     VWKALAPAMPELLTAGSFVSVCATAIACTHPDTGEPTLLVEPQAGGWGASVLKDGEHG
                     LVSVGDGETYVIPAEVCEQRYGIRVERFGFDVVEAGAGRRRGGRGLVREYRMLTDGSL
                     TVGFGRHLSPPWGVDGGHDGSPNYVEVVRADGSHERFGKVARYPLREGDLVRLVTGTG
                     GGAGDPRDRERELVRDDLEEGIVTEREARDVYGAAPG"
BASE COUNT         1947 a         4551 c         4512 g         1880 t
ORIGIN      
        1 aaatggatcc gatgcgctgc tcgggtccga cggtggcgac cgtctgctcg gcggacgcgg
       61 ggacgaccgg ctccgcggtg gcgtgggtgc ggacgtgctc gacgattccc tccgcgtcgc
      121 acagggtgtg tccccggaca ccggcgccga tctcctgtcg ggtgggccgg gcgacgatcg
      181 tctgcggggc ggcgacgagg tccgggcgaa cgacacgctg catgggggag agggccacga
      241 tgcctgctcg gccgatcccg gcgatcggat ctcggggtgc cgtccctagc actgcgcgat
      301 gaaatttgcc gggcgagccg tcgcaactag ggtcagccga ctgccgggcc tacgttaggt
      361 cttcttctat tgggggcgga ccaagaggtc ggcgcccgtc gcggtctcct tggcgtccag
      421 gaccgcgtcc tcgagctcgg gggcgaggtc ccttgctacg acgaccgtga ggccgcgcgc
      481 gtctgtgggc tcgtcgttcg gtcctgcctc tctgatcggc tcgactccaa ccgacgtgcg
      541 gccgtccggc tgaggattcg gaacgaggcg gatcccgccg ccgccgcgct cctcttgctc
      601 gagaaccttc ttgaacactg atacggctgc gtcggtgacc tgaagcatcg ctggcctcct
      661 tccgacgtct ctccagcttt acccccggcg ctcgacggca cacgcggacc ggccggttct
      721 gcgagtgggg gatccgccgg tggatacggc gtctgacggc gccctggccg gccatggttt
      781 gacgaacttc ctttgccgag tgtgaccttg ttcacaatga gtcgaacgtg cgttcgagta
      841 gtgtcgagca catgagcatg ctgcgatcgg cgctcgacga gctgcgcggg gacgacctcc
      901 cctcattgtc ggacgaggcg ctcgccggtc acctggatga gatcgagcgc gcgggacgca
      961 tcctcgagtt cgagcgcggt cgccgcctgg cggagctcgg acgtcgaaag gtctactcgg
     1021 tcgacggcca cctgtcggcg gcggcgtggt tggcgcaacg gcaagggctg tcgcgctcgg
     1081 cagccgaggg ccgcgtccgt cgcgctcgtg cgctcgaagc gatgcccgag gtggcccgcg
     1141 cgttcgggga aggtgaggtc tccgaatctg ccgtccatgt gctcgcatcc gcccaggagg
     1201 ctgctccaga ggcgttcgcg cgatccgaac ccgcgctcgt cgaggctgcg cgctcgatgc
     1261 ccttcggcga gctccgccgg gtgaccgaaa cgtggcggat cgccgccgat cccgcacggg
     1321 cgctcgttga cgaggatcgt cgccacgagc gccggcgctt ggacatctgt cctaccgagg
     1381 acggcatgac gggcgtgcgg ggagagctgg acgcggagga cggccagggg ctcatcaccg
     1441 cgatccgggc gcagatggac gccgagacgc gttcggcgag cggaccagac cttcgcacgc
     1501 ccacccagcg ccgagccgat gcactcggcg agatctgtcg tcagtggctg gcctcacgcg
     1561 agcgcccgat catcggcggc gagcgcccgc acgtcgtggt cacgatcgac ctggagacgt
     1621 tgatcgagcg ttccggacag cgctcgaagc tgcaagacac cggccagatc acgcccgaaa
     1681 ccgctcgccg cctcgcctgc gacgccgatg tcactcgagt gatcacgcgg gcgggctccg
     1741 agccgctcga gctcggccgt cgcaccaagg tcgtgccgcc cggcctccga cgcgcggtcg
     1801 ccgttcggga ccgcggatgt cggtttccag gctgtggacg gccacccggc tggtgcgacg
     1861 cccatcacgt tcgccactgg gccgacggtg gcgagacgtc gctcggcaac ctcgtgctgc
     1921 tctgccggcc acatcaccga gcgatccacc gcggcttctg cgtcgagatg atcgacggtc
     1981 gaccgctgtt ctgccggccg gacggcacga tgctcgagga tcgcgcaccg ccccccgacc
     2041 tgaccggggg tgcgctggct aaatctcgcc gaccaggatc tcctccgccc ggtggcacgc
     2101 gacgactcgg ccggcatcga ccacccggag ctgcggcacc tcggttcggc accgatcgat
     2161 cgcgtagggg cacctcggat ggaacgggca tccgggcggc gggttcgcgg gattcggcac
     2221 cgtgccgggt agcacgatcc tcgaccgacg ggcggcgagg gcgatctccg ggacggccga
     2281 gcgcagcgcc agcgtgtacg ggtgtgctgg ccgcgcgagg atctgctcgg tcgcgccgtg
     2341 ctcgacgatt cgcccgagat agagcaccgc ggtctcctcg cagagctgct cgaccacggc
     2401 caggttgtgc gagatcagca cgtaggcgag cccgcgttcg cgccgcagct tggcgatgag
     2461 ctcgagaatc cgcgcctgca ccgtgacgtc gagcgcactc gtcggctcgt cgagaaccag
     2521 caccctcggc tcgaccgaga gcgcgcgggc gatcgccacg cgttggcgtt ggccacccga
     2581 gagctggtgc gggtagcggc cggccatctc ggggtcgagg ccgacctcgg cgagcagcga
     2641 cgcgatccgt tcgtggacct cggcccgggg aacgatgccg tgcgcccgca ggggttcggc
     2701 gatcgtcgac ccgatcttgg cgcgcgggtc gagcgacgtc gtggggtcct ggaacacaat
     2761 ctgcacgtct cgccggtacg cgcggagcgc ccggctcgag cccacgacct cgctcccgtc
     2821 caggcggatc gcgccggagg tggccgagac gagccgcatg agcgcccgcg cgatcgtggt
     2881 cttgcccgaa ccgctctcgc cgacaagcgc cagcccgtag gggccggccg gcagccggaa
     2941 cgagacgccg tcgacggctc gcacggggcc gaactcgacg acgaggtcct cgacctcgag
     3001 gagggccgtc tcgaccgcgg tcaccgggct cacgcggtcc ccgcgccgat cttcgggacc
     3061 gccgccacga gccgcctcgt gtactggtcc gccggctcgc cgaggacgcg cgccgtgggc
     3121 ccctcctcga cgatccgccc gagctccatc acggcgacgc gatcgcagag ctccgcgatc
     3181 acggcgaggt cgtggctgat gaacaggacg ctcatcccgc gctcctcgcg cacgcggcgc
     3241 aggaggtcga ggatctcggc ctggacggtg acgtcgagcg cgctcgtcgg ctcgtccgcg
     3301 agcagcacct cggagcgcag cgcgagcgcc atcgcgatcg cgatccgctg cgcctgcccg
     3361 cccgagagct cgtgcgggta ccgccggagc aggtcgggag gaaggagcac ctcgcggaac
     3421 gcgttcgacg cgagctccga cgcctcgcgc ctgccgacac cgtgcagctt gagcgttcgc
     3481 tcgaagagct cccccacctg gagcagcggg tcgagcgagg acacggggct ctggaagatc
     3541 atcgagatgc ggctcccgcg gatctcgcgc agccgtctct cgggcgcgcc gacgagctcc
     3601 tcgccgtcca ggcggacgct tccctcgacc cgcgcgccta gggtcgacgc gaggccgagc
     3661 atagccagcg ccgtgatcga cttgcccgag ccgctctcgc cggccaggcc gaggatctcg
     3721 cccgggccga gctcgagacg ctcgaggtcg acgaccatgc ggggcccgaa cgcgatccgc
     3781 aggtcctgca gcgagagcct cgactcggtc atcgcgtcgc cagcacgcgc ctgggatcca
     3841 gcacgtcgcg gaggccgtcg ccgagcaggt tgaaggccac ggccgtcagg aagatcgcgg
     3901 cgcccgggaa ggtgaccagc caccactgcg tcgtgaagaa cgcctgcccc tggctcacca
     3961 tgagccccca gtccggcgtg gggtcctggg cgccgagacc gaggaacgac agcgccgacg
     4021 cggtgaggat cacgccgccc acgtcgaggg aaacctggac gatgatcggg gtgatcgagt
     4081 tcgggagcac gtgccggagc aggatcgtgc ggtggggaac gccggtcgcc ttggcgctct
     4141 cgacgaacgg acgcccggtc accgacgcgg cctgcccgcg gacgatccgc gtgtaccacg
     4201 gccaccacgt gaccgagatc gcgatgaccg tgttcgtgat cgagggctgc atcaccgccg
     4261 cgaacgcaag cgcgagcagg agcgccggga acgccaagaa gacgtcggtg acgcgcgaga
     4321 tcacctcgtc gatccagcct ccgaaatacc cggcggcgat cccgagcggc accccgatca
     4381 gcacggagat cgccagcacg agcacggcga tcaacggcga gatgcgcgcg ccgtagacca
     4441 cgcgcgagaa cacgtcccgc cccacctggt ccgtcccgaa gtagtgctgg gccgagggcg
     4501 gctcgagcgt ctggaggggg tgcacggcgc tcccggcgtc ctccgggtac gtcgcgacga
     4561 gcggcgcgag cacggccacg aggaagatcg cgaggcacat cgcggcgccg accgcgagaa
     4621 gcgggttccg ccggatcacc ccggggacgc tgcggaggcg gccgggcggc gcgaggacct
     4681 cctcgcgtga ctcgacgtcc gggtagctcg ggaccctcga catcagctca ccttgatccg
     4741 cgggtcgatc acggcctgga cgatgtcgac gagcaggttg ccgatcacgt agacgatcgc
     4801 gacgaacagc gtgacgccga ggatcgccgg cgtgtcgagc gccccgatcg cgtccgcggc
     4861 gtagctgccc aggcccggcc ggttgaagat cgcctcgacg aggaacgtgt tcacgagcgc
     4921 gtacgcgaac acgagcgcga gcagcgcgac gaccgggctc cacgcgtgcc ggagcgcgaa
     4981 gcgcccgaac acgctccgct cggggaagcc gagcgcccgc accatccgca cgtggtcctc
     5041 gccgatcgtg tccagcaccg aggcgcgcac catccgcgtg atgacaccgg cggggtacgc
     5101 cgcgatcacg atcgcgggca ggatgatgtg ccggagcgag cttccgaaca cggcccagtt
     5161 gccggtcagc agggcgtcga cgatcggcat gttcgtgatg ctgtggagcg ggctcgtgta
     5221 gtcgaggttc gggtcgtacg taccggcgac cggcagcagg tgcagccact ggaagaacac
     5281 cagctgcagg acgatcgcca gccagaacga gggcatcgag acgcccacga cggccacgag
     5341 ccgcgaaccg atgtccggga ccttcccctt ccatcgcgca gccacgagcc cgagcggcac
     5401 gcccacggcc agcgccagca gcgtcgcgaa cgtgacgagc tcgatcgacg cgggggcgcg
     5461 ccgcccgatg tcgccgagca ccggtcggcg ggtccggagc gagacgcccc agtcgccgct
     5521 cgcgaccccg ccgacgtact cggcgatctg tacggggatc gggcgatcga gcccgagctc
     5581 ctccctggcc ttctcgatct gctcggggcg ggctcggggg cccgcccacg tcgccgcggg
     5641 atcgcccggc acgacgcgcg cgatgacgaa cgtgagcacg acgacgccca ggacgaccag
     5701 cgcggcgagc gccagccgcc ggacgacgaa ccgcgtcacg cggcgggcgt cagctcgtag
     5761 ccgaagacca cgttcgggta ggccgggttg tcgacgaagc cgtcgaccga gtcgagcatc
     5821 gcgcgctggt acacctgcgt gtacagcggg atcgccggcg cgtcctcgaa cagggtccgc
     5881 tgcatgtccc cgtacatctg ggtcgcccgg tcccggtcgg tggccgcgac gaccgaggcc
     5941 cggtcgatct ccgaatccag agcggggttc gagtaatacg ccaggttgaa gaacggttct
     6001 ttctcggtgc ggaacaggtt gtagaaccag gagacggggt ccgggtagtc cggccaccag
     6061 tagaagatga agatgtcctg ccggtcggcg gggttcgcgg acttgccctg atcccactgc
     6121 gtctgccact gcaggccgcg tacgtcaacg ttgatgttca ggtcggccag gttcgacttc
     6181 atgagcgtcg agaccagctc ctcgtcggcg tcgcccttga ggtaggtgag cgagaggtcg
     6241 atcgggtcgc cgctcgggcc gaacccggcc tgctccagca tcgttcgcgc ctcgtcgacg
     6301 ttctgctggt actcgagccc gtcggtgtgg ccccacaggc ccggcggcac cacgccagag
     6361 agcgggtccg cggcgccctc gagggcggcg atcatcccgt cgtagtcgat cgccttcgtc
     6421 accgcgtcgc ggaccagcgg atcggcgagc gggccgtcgg ccgtgttgag catgccgagc
     6481 agcgtttgcc acgagggagc gctctcggtc tcgaagccgt cctgtccctc gaaggaggcc
     6541 cacagctgcg ggctcatccg ctggaccatc gagacctcgc ccgcgcgcag gagctgcgcg
     6601 gcggtgttcg cctcgggcgt cacgcggaag acgtagcgct cgtagtgatc cgcgtcgtcc
     6661 cacccgcccc agtagtccgg gtaggcctcg aggcgcagct cgatctcgtc accggggttg
     6721 tactcggaga ccatgtacgg gccggtgccg gcgtcgttgc cgtcggcgaa ccagtcgccg
     6781 agatcctgac cgccgctcgc cgtcgtgtcg tagatgtagg ccgagtacgt gccggacgac
     6841 acgaggtcga gcggcgacgg gtacgacagg tggaacacga gcgtggtgtc gtcgggcgcg
     6901 tcgatcttct tcaccgctcc ccagatgtag gccgcgccct cgccgagctt catcgtccgt
     6961 tcgatcgcgg ccttcgccgc ctcggcggtg agcgggttgc ccgtgtggaa ggtcacgccc
     7021 gggtgaagcg tgaacgtcca cgtcaggccg tccttcgacg attcccacga gtcggccagg
     7081 agcggttcga cctcctgggt gtcggcgttg tagcgcgtga gcgtctcgta gatgttgtgg
     7141 agcgcgatga cctcgttcga atagccggtc gccgggtccc agccgatcat gacctcggag
     7201 ttgaccgagt aggtgaacgt gtccggctcg gcgttcgtcc cgcctccggt cgtgctccct
     7261 ccaccctcgt cttccgtgca ggcggccgcg gcgacggcga acgcggccag caccaccacc
     7321 catcggaacc gggcagatcg catggcctcc cctccccgtt cggttcgtcc ggtgcgtccg
     7381 gcgtcaaccc caggcgagcg gcccgccggc cttgaccctt atacgtaccg caggatcggt
     7441 gaggtatgcc aggggcacct cctcgatctc gacgacctcg acgctctcgc gggcggcccc
     7501 ggcggagacg gcgcgctcga tcgcctcctg cgatgcctcg gcgatggcgg cctcgcggcc
     7561 cgcgacgccc gggtgaaaga cgcggtcgac ctcgccgctc accgacgcga tcgcggcccc
     7621 gatcgcgttc gccacgtcgg agtggtcggg ccggaggacc tcgctggtgc ccgtcaggtc
     7681 atcgggcacg agcggcgcac cgccgccgac ggcgacgagc gtcacgggct cgcgcgacgt
     7741 cttcacccga tcgatcgcgt ccgccagcgc ggcgtcggag cgggcgatgg cggcggcgag
     7801 cagcggcccg tgccgggcga ggcggctcgc gtcgccgagg gcgaaccgtt gcgcggcgac
     7861 gcccgcgtcg gtgagcgtgg gcgtcgagcc gccgaagacg agcgcctcct cgaggatccg
     7921 gtagccgacg ctctcggggc cgagcgcgtc ctcggtcacg atcgttcccc cgccgagcgc
     7981 gatcgtcacg agatcgggca tccggaagtt cgtgcggatc ccgccgatct ccacgccgaa
     8041 cgaggactcg cgcgggaagc cccgaaccag cacgcccacg tcggtcgagg tcccgccgac
     8101 gtcgaccacg atcgcgtcgg tccggccggt gaggtaggcc gcgccccgga tgctgttcgc
     8161 cgggccgctg ccgatcgtga gcacggggaa gcggatcgcg aactccagat ccatgagcgt
     8221 gccgtcgttc tgcgcgaaga acgtgacggg ccgcaggccg ttgcgttcga gggcgccggc
     8281 gatcgcgctc gtgacgccgc gcgcgacgtc gacgagcgac gcgttcagga cggtggcgtt
     8341 ctcgcgctcg agcaggccga gggagccgat ctgggagctg agcgagatcg gcaggtcgcc
     8401 gagctccttc cgcacgatct cctcggccgc gcgctcgtgc tcgtcggaga cgggggcgaa
     8461 gacgctcgcg atcgcgatcg cgtccgctcg accggccacc tcgcccgcga agcgcgcgac
     8521 ggcctcggcg tcgagcggcg acagctcgcg cccgtcgaac tcgatcccgc cgtgcacgat
     8581 ccgttcgccg gcggagacgg cctcgcgcag gtcggccggc cacccgaaga gaggccggat
     8641 cgcctgcgtc gccggtccgc ccagtcgcag cacgccgacg cgccggagac cgcgtcgctc
     8701 gaggaccgcg ttcgtcgcgt gcgtcgtccc gaacatcgcg tgcgtgatcc gcgcgcggtc
     8761 gacctgctcg acgacgccgg cgagcgcggc ctcgatgccc ttcgtgacgt ccggcgtggt
     8821 cggcaccttc gccttcgcga ggaggcggtc ctcggcatcg agcacgacgg cgtccgtgtt
     8881 cgtcccgccc acgtcgacgc cgagccggag gtcaggcgag ggcatgcagg tcctccaccg
     8941 gcgcgtagtc gaggtcgtaa ccgaagtgtc gcggcccggc gagctcgagg ccgcgagccg
     9001 tccgccagat cggatcgcac gggaaggcga tgacggtcac acgctggccg tagcggaggc
     9061 gttcggtcga gatcgcgttc gcggactgcg agtcgagcac cgtgatgatg tcggggacca
     9121 tcgcgagtgc gcgttccccc tccagcgcga cgaggttctc gttctggatc tcgagccgga
     9181 gtacgcgccc cgcatcggcg tcgagcccct cgatcgtcgc cgaccctcgg acgaaccccg
     9241 cggtcgtgcg ccgttcgacg tcgacgatct tgcccacgat gagacgagcg gcgtggatct
     9301 cgtgcgcgag ggcctcgacc ggctcgtgct cggcttccgc gacgacgctg ccgagccgga
     9361 tcgcgagcga cacggcgtgg ttcacgacgg ctcccgcctt cgcctgcccg acggtcagct
     9421 gatactcgct tgaccacgac atgccgccga actcgaccgc ggccgcgcgc tccatccgct
     9481 ccatccagtc gccgtcggtg gctcggaaga cgatgacgtt gccgcgctcg tcggtcatga
     9541 cggccgggtt gggcgagatg cctgcgatct ccatggtgac ctgcgggatc tcggggaacg
     9601 cgcggcccat gccgtcggcg tcgacgacgg gcaggccgat cctcgcggcc cacaccacgg
     9661 ggatgatgcc gttcgagccg ccgatctcgg ccgacatcag cgcgacgacc gggcggttcc
     9721 acagggtttc gaccatctcg cgcagtcggc cgccctcgtc gccgctctcg atcttctcga
     9781 tcgagacggt gggggcgccc acgccgccgc agggcatgag cagggcctcg tccggaagct
     9841 cctcgaggtc gacgagtgga acgggaccat ggtcctcgac cgcctggagc gacgcgagca
     9901 ggccgatctc ggtgtcccca cccccgcccg cgccgaggat cgcgcaccca cgcgcgagcg
     9961 acggcagcga cgaagcatcg agctgcatgg cgcggatgct agccagtaaa ccaggcgccc
    10021 atcaggagaa ggtgtcatgc cgtctctgcg gagggcgtcg tcagcgcctt catgaggaga
    10081 gtggatcagc aggccgctcc gccgagggat caagcgatca gggcgaaacc catcgcgagc
    10141 agccagaccg accatgccat gtagaggatc gggatcgccg acaaccctcg gttgcggcga
    10201 cgattcaggc cgtatccagc tcctcgatcg tcgcctttga tggcgagcgc tcgccccgca
    10261 gacgcgtcct cgttcgctcc ccttcgcgca tgaccctgag cacgctaccg cccgcgatct
    10321 tcataaggtc ctcttcgcca tagcctcggc tcgccagctc ctccaacagc accggatacc
    10381 gggacacgtc ctccagccct tcaggcatgg tcggtgcccc gtcgaagtcg ctcccgatcc
    10441 cgacgtggtc gatcccggcc acctcgcgga cgtgatcgat gtggttggcc acgtcggaca
    10501 cagttgcggt cgggcctggg ttcatctcga accatgcgtc catcgccttt cgaacggcgt
    10561 ccggatcgtc gggatgctca gcgcgaacgc gacgagcctc ggccaacccc accaggttga
    10621 actcggctcc ctcagccgcg acgaacgatg gcacgaacgt caccatgacc acgccaccag
    10681 ttcgaccgac cagctcgagg acgtcgtctg gcacgttcct cggcacgtcg cacagggccc
    10741 gggccgagga gtgagagaag atcaccggcg ccgcgctcac ctcgatcgcc tgtcgcatcg
    10801 tatcgtccga ggtgtggctg aggtccacga gccagccgca ccggttcacc tcgcggacga
    10861 cctcctcccc gaacctggtc agcccgccgt gcgagtgctc gccggtggcc gagtcggccc
    10921 accgcgtatc gtcgttgtgc gtgagcgtca ggtagcccat gctgaactcg gccaggaccc
    10981 ggagcgtccc gagcgagccg ccgattgcgt ggccgccctc gacaccgatc atcgacgcga
    11041 cccgtccggc ggcggcgatc cgctcgacgt cgtcggccga cctggcgagc tcgagccgat
    11101 cggggttggt gcggacaaga tcgagcagcg cgtcgatctg ctcgaacgtc atggtcacgg
    11161 cgcggtccgc cggcaggtcc gagggcacgt acaccgacca aaactggccg cctacgccgc
    11221 ctgcggccat gcgcggcagg tcggtcatga gggtcgggtc ggccggatcg cggccctgcc
    11281 gaagctccca cagcaggtcg ttgtggccgt cgatgagggg agctggtcga aggagatcaa
    11341 gcatggcggt cggatgctag cgggcgatcc ccatcgacag gcacccgact aaagaagcta
    11401 gacccaatcg gacgggcccc gcaccagggc gcataaggtt cccgtctggg tgaatcgggc
    11461 agtccgagga gggggttcta agaaatctgg tctgcacccg ggttccggct tgctcggttg
    11521 cggctgcggg tggtccgtta gcacggttcc ccacatctcg gtggcaacag tacgcccgag
    11581 gcgataagca acccgctcga tcagccagga gcagcaccgt acacgtcgcg tgcctcgcgt
    11641 tcggtcacga tgccctcctc gaggtcatcg cgaacgagtt cgcgttctct gtcgcggggg
    11701 tcgccggcgc cgccgcccgt tccggtgacg agccggacga ggtcaccctc ccgcagcggg
    11761 tagcgcgcga ccttgccgaa ccgctcatga gagccgtccg cccggacgac ctcgacgtag
    11821 ttcggcgagc cgtcgtggcc gccgtccacg ccccaggggg gagacaggtg acggccgaag
    11881 cccacggtga ggctgccgtc ggtcaacatg cggtactcgc gcacgagtcc gcgcccgccg
    11941 cgtcgccggc ccgcgccggc ctcgacgaca tcgaacccga accgctcgac ccggatccca
    12001 taccgctgct cgcacacctc ggccgggatg acatacgtct cgccgtctcc gaccgagacg
    12061 aggccgtgct caccgtcctt gagcacgctc gcgccccagc cgcccgcctg cggctcgacc
    12121 aatagcgtgg gttcgccggt gtccgggtgt gtgcacgcga tcgccgtcgc gcacacggag
    12181 acgaagctgc cagccgtgag gagctcgggc atcgcgggcg ccagtgcctt ccagacgaga
    12241 tcggtcgcgg cctcggtcgc ctcgaagtac agcgcgacgg gcgccggccg ctgggccgtg
    12301 aagatcgtgc cgagcggaca gaccacctcg aacggccgga accatgcgtc ggagccgggt
    12361 tcgttcggat ccgtgatcgc cttgaacacc gttcggatcc cggagacgag cccactccgc
    12421 gtgcagttca cgggccccgg aacctgggga tgggtaccgg tgaagtccgc gacgacgcgc
    12481 tcgccgtcga tctcgacgcg cacacggatc gggaacgggc cgttgccaag gccgtcctcg
    12541 tcgatgaagt cctcggcctc gaacacgccg cgggggagac gcccgacggc ggccctagac
    12601 agtgcctcgc tccgttcgtg cgctgcggcc atcgccgacg tgacgtcctc gacgccgtac
    12661 cgttggcaga gctcgagcag tcggcgctcg gcgacctcga gacaggccgc gaacgcggtc
    12721 agatcgccga tcgtcatgtc gggcaggcgg ctgttcgcgc cgatcatggc gacgaggtcg
    12781 cggtcgagct ggcctgcgcg gacgacgcgg acgaacggca gctgcagtcc ctcctggaag
    12841 atctccaccg agtcggcggt ccacgacccg gggtccttgc caccgacctc
//
LOCUS       DATBRK010000069         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_153362, whole genome shotgun sequence.
ACCESSION   DATBRK010000069 DATBRK010000000
VERSION     DATBRK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153362"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VIH70_02160"
     CDS             <1..129
                     /locus_tag="VIH70_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY8115199.1"
                     /translation="IPIIEHLTSLETLPAEGFDFFAVPQRVEGMGTFPVRAFAMVP"
     gene            complement(336..536)
                     /locus_tag="VIH70_02165"
     CDS             complement(336..536)
                     /locus_tag="VIH70_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115200.1"
                     /translation="MTWNPAGDHHHPTDVTYVKPDGSQATISAHRLPFVIEGDVTVET
                     MGVVVCSGVCTKCGQRVMPSTR"
     gene            complement(685..864)
                     /locus_tag="VIH70_02170"
     CDS             complement(685..864)
                     /locus_tag="VIH70_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115201.1"
                     /translation="MTRLLERSTVLALAALVAFNVAMMVAWSAAGPSRDALVVAGWIA
                     GDAVLGVIALFVTDR"
BASE COUNT          297 a          520 c          534 g          277 t
ORIGIN      
        1 atcccgatca tcgagcacct cacgagcctc gaaacgcttc ctgcggaggg gttcgatttc
       61 ttcgccgttc cgcagcgggt tgaggggatg ggcaccttcc ccgttcgggc tttcgcgatg
      121 gtgccatgac cttaacctga catgcgacgg ggcaagggcg ggtaggagcc cgatgccggg
      181 gttgctagcg gaactgaaat ggccctctgc ccgcgggttt ggggcgttcg cggcctggca
      241 ggagccggaa ctcttcacca ccgaagtgat gccgcgggag tccgatcgct gagttcctcg
      301 gtctcagcca ccagcgggcc agaagatcga atgccctagc gggtggaggg cataacgcgc
      361 tgcccgcact tcgtgcacac gcccgagcag acgacgacgc ccatcgtctc gacggtcaca
      421 tcaccctcga tcacgaacgg aagccggtgt gccgagatcg tcgcctgcga tccgtcaggc
      481 ttcacgtagg tcacgtccgt cgggtggtgg tggtccccag cgggattcca cgtcatgtga
      541 gtgattctgt agccctccac gaccaggatc ttacgcccgc tgtttcccca agcctgtcgg
      601 ccttgagggt ccagagccgc ctatgggatc ggcgcgaggt cgaggacgtg gcctgaagcg
      661 ctgcgccgcg gggaggccct ggcgctagcg gtccgtgacg aacagagcga tcacgcccag
      721 gacggcgtcc ccagcaatcc agcccgcaac gacaagcgcg tcgcgggaag gaccggcagc
      781 ggaccacgcg accatcatcg ccacgttgaa ggcaaccaga gcggccaggg ctaggaccgt
      841 gcttcgttct aggagccggg tcatcgctct caacctaggg gcgggctgct cccgggtcaa
      901 caccggcagg tccaggcctg ggcggagcgc tggcgccgcg agaagcgtgg gggaacccgc
      961 taccgacccg caccttcgct gagctcctta taagactgcg aggaggaccg gacgctccgg
     1021 gcgatgctcg tcgggatgct gcggaaaatc cgaccactag cttcggctac cagataaagc
     1081 ggcgccaacg aagacaggac caccgagcgg gggtccttcg accacatgcg cgacgatgca
     1141 agtcacattg tctcgtgcac cgctgctgag cgcctgaccg ataagtcgct gggcgcactc
     1201 atctgtgtgg ctcgatgaag tcaccacctc cgcgatcaca tcatccgagt agaggccatc
     1261 cgaacagaga cgcagcaggt cacctggttg gacggtcccg accctcgcct ccggcgaggt
     1321 tcgtcgcgac gcgtcgtggt agtcggcggg acgaatgcgg gacgagcggc cgggctgact
     1381 cggtgttcag actgcacagg tggcctgacc tgcgcctttg cttgtagtcc caacggggtt
     1441 cgaacccgtg tctccacctt gagaggttga gccgagccga gtccgttacc tgcggcgaag
     1501 gcggaaatgg atcactgacc tgggaattcg gctccggtcg attctggtca gtacccctcg
     1561 cgtttcgacg tcatgcggga taaacacggg atcaccggac gccgatggtg acaccaccct
     1621 gctgccag
//
LOCUS       DATBRK010000070         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1451204, whole genome shotgun sequence.
ACCESSION   DATBRK010000070 DATBRK010000000
VERSION     DATBRK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451204"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..961
                     /locus_tag="VIH70_02175"
     CDS             107..961
                     /locus_tag="VIH70_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY8115202.1"
                     /translation="MNVPFRLVDVFTPRPLAGNQLCVVPEPGQLDDAGMQAIAKEIGF
                     SETTFVTEAAGDRYAMRIFTPGQELPFAGHPTLGTAFVLVSEGRVPTPATQVIAAGEI
                     PVEVDVASGTAWMTQLPAEFGPIFPDRDLIASAIGLTTAELDSGRPVRTVSTGLPTTI
                     VPVRELDMLRRATRNERLVGEAVGASGGRDLYLFAHTAEGVAARMFDSEFGIGEDPAT
                     GSAAGPLGAYLAEYGDLGETRSITIRQGEHVERPSELHVEVDREGDAWRVRVGGGVHI
                     VGRGAFEL"
     gene            1019..1426
                     /locus_tag="VIH70_02180"
     CDS             1019..1426
                     /locus_tag="VIH70_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY8115203.1"
                     /translation="MRVRVVVLLVLITGSLAWVGTQALSGNLVYYVTPTELLRQHPAP
                     DERLRLGGQVVPGSLHDVADGIDFVVTDGTTRMSVVHTGSTPSLFQSGTGVVLEGTYG
                     TDGAFHSDNMLVKHGEQYRPPDPGETPAPDVVS"
     gene            1426..>1644
                     /locus_tag="VIH70_02185"
     CDS             1426..>1644
                     /locus_tag="VIH70_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY8115204.1"
                     /translation="MPALVGTLSLAMALALALYGGIAAIVGARTGRPRVVESARTAAY
                     SLFALVVVANLAMLAALLANDFSLRYVAS"
BASE COUNT          237 a          569 c          590 g          248 t
ORIGIN      
        1 cgaacccgac gacggcgacc gcgagcgggc cgacggctcg agccccgtcc cggaaggtcc
       61 gttcggccat gaccaacggt agcaatgggt cggtacagtg cccggcgtga acgttccctt
      121 ccggctcgtc gacgtgttca cgccgcgacc gctcgccggg aaccagctct gtgtcgtccc
      181 agaacccggc cagctcgacg acgccgggat gcaggcgatc gcgaaggaga tcgggttctc
      241 cgagaccacc ttcgtgaccg aggcggccgg cgaccgctac gcgatgcgga tcttcacgcc
      301 ggggcaggag ctgccgttcg cgggacaccc gacgctcggg acggcgttcg tgttggtgag
      361 cgagggacgg gtgccgacgc cggccacgca ggtcatcgcc gcgggagaga tcccggtcga
      421 ggtggacgtc gcatccggca ccgcctggat gacacagctg cccgccgagt tcgggcccat
      481 ctttcccgac cgcgacctca tcgcgagcgc gatcgggctc accaccgctg aactggattc
      541 gggtcgtccg gttcggacgg tctcgaccgg cctgcccacc acgatcgtgc ccgtacgaga
      601 gctggacatg ctccgacgag cgacgaggaa cgagcgcctc gtcggagagg ccgtaggggc
      661 aagcggcggc cgggacctct acctgttcgc gcacacggcc gagggcgtgg ccgctcggat
      721 gttcgattcg gagttcggca tcggcgagga tccggcgacc gggtccgccg ccggcccgct
      781 cggggcctac ctcgccgagt acggcgatct gggggagacg cggtcgatca ccatccgcca
      841 gggagagcac gtcgagcgac cgagcgagct ccatgtcgag gtggatcgtg agggcgacgc
      901 gtggcgggtt cgggtgggtg gcggggtgca catcgtcggt cggggagcgt tcgagctctg
      961 agcccgagct cgttcacgtg cctcgacgga aacggaggca ccctcgtagg ctctgtccgt
     1021 gcgcgttcgc gtcgtcgtcc tgctcgtgct gatcaccggc agcctcgcat gggtcgggac
     1081 gcaggccctg tccggcaacc tcgtctacta cgtcacaccc accgagctcc tccggcagca
     1141 cccggctccc gacgaacgcc ttcggctcgg gggacaggtc gtgccggggt cgctgcacga
     1201 cgtcgccgac ggcatcgact tcgtcgtgac cgacggcacc accaggatga gcgtggtcca
     1261 cacgggatcg acgccgtccc tgttccagag cggcaccggc gtcgtgctcg agggcaccta
     1321 cgggacggac ggcgccttcc actcagacaa catgctcgtc aagcacggtg agcagtaccg
     1381 gccgccggat ccgggggaga cgcccgcacc ggacgtggtg agctgatgcc ggcgctggtc
     1441 gggacgctct cgctcgccat ggcgctcgcc ctcgcgctgt acggcgggat cgccgcgatc
     1501 gtgggcgctc ggaccggccg gccgcgcgtg gtcgagagcg cgcggacggc ggcgtactcg
     1561 ctgttcgcgc tcgtcgtcgt ggcgaacctc gcgatgctcg cggcgctgct cgcgaacgac
     1621 ttctcgctcc gctacgtcgc gtcg
//
LOCUS       DATBRK010000071         4299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1595804, whole genome shotgun sequence.
ACCESSION   DATBRK010000071 DATBRK010000000
VERSION     DATBRK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595804"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VIH70_02190"
     CDS             complement(<1..1137)
                     /locus_tag="VIH70_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8115205.1"
                     /translation="MTEVAQTRYAKTLDGGYIAYKLLGDPSSENEVAVIGSIAANVEL
                     FFDFEPAARSWLELASFSRLSLHDRRGTGLSDDMGGLPDLETRAADLLAVLDAAGLRR
                     PILLASADGGMVAALLAATHPDRVAGLVWFFAQARSVKTRGWPFGRDPDDVDLLAAEA
                     ERAWGTEDFVRRMYAHAEMDDDVIRFLARMQRHACGPATAVKFIRLLHDYDVREILPT
                     LRIPVLALGTEGYPGEWVVEQARATAALIPGAVCRSMPARASRLWAPAVIEATFDFCG
                     IQRPPPELDSILSTVLFTDIVGSVEKQAVLGDHAWKELVMRYRSIVRKTLERWRGREN
                     DTAGDGFYAAFDGPARAIRCALEVAERVRDLGIEIRAGVHTGECE"
     gene            1443..2810
                     /locus_tag="VIH70_02195"
     CDS             1443..2810
                     /locus_tag="VIH70_02195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8115206.1"
                     /translation="MVETAPPSPSGRRARDLAAATPGTRNRYADLLRVASIFVVVSGH
                     WLMAVLEYEDGSFVGKNLLEIATWTHILTWIFQVMPIFFIVGGFTNAGSWRSATRRGV
                     SYATWLRARSARLLGPALVFVAFWTVLPMLTVLVGLPSGMARTGGREVALPIWFLAVY
                     LLTVAAAPPLLAAHQRYGVRVLVALAVGAATVDAVRYGLDVGWIGVVNYAFVWLAILE
                     LGFLWREGALTRVRWIPWAMAGTGLAALALLTTVFDYPVSMIGLSHAQRSNTLPPTTA
                     LLALAVWQCGAMLIFEKPANRWLLRPRLWLGIVVANSMVMTFYLWNMTAAVLAAVILL
                     PTGIAPQFEELSGAWWLWRLAWIAACAICLVPLLFAFRWAERPGAPPPPAPAGWSGLV
                     MSLAGVACASAGMSIIAAAAFPVQGEVLAMPLLGVAFLVVGAILLHVNPFSPLSKRSA
                     QSLLG"
     gene            2874..3266
                     /locus_tag="VIH70_02200"
     CDS             2874..3266
                     /locus_tag="VIH70_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8115207.1"
                     /translation="MLGWVEDIERATLENENFRTVLHTGEHVQLTVMQLQPGEDIGRE
                     RHPHTDQFLRIESGTGRVEFGRNEDAVDETHDVEEDWAIVVPAGVWHNVVNTGDGDLK
                     LYSLYAPPEHPDGTVHRTKADAAAAEHD"
     gene            3337..3846
                     /locus_tag="VIH70_02205"
     CDS             3337..3846
                     /locus_tag="VIH70_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115208.1"
                     /translation="MNLLGYLNDHLAGAAAGIQLAERCRDRDPESELGQMLQGLLLEI
                     RHDRDVLERVIRVLGGSPDTIKRGAALGAERISSLRMWLPMVGPGSRASARLEEIEVL
                     SLGIEGKRLLWAALAQLSSADERLSGFAFPDLKRRARSQRDRLERFRLRLAAEAFGPP
                     KVAPMRATA"
     gene            complement(4054..>4299)
                     /locus_tag="VIH70_02210"
     CDS             complement(4054..>4299)
                     /locus_tag="VIH70_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY8115209.1"
                     /translation="TRPEIAEDTMRECVDLGIDRVWMHRSFGGGSVSDAATEYGRQHG
                     VTVIDGGCPLMFEPTADAGHKVMRVVFSATGKVPRKV"
BASE COUNT          640 a         1515 c         1470 g          674 t
ORIGIN      
        1 ctcgcattcg ccggtatgca cgccggcccg gatctcgatc cccagatcgc ggacccgttc
       61 ggccacctcc agcgcgcagc ggatcgcgcg ggcgggaccg tcgaacgcgg cgtagaagcc
      121 gtcgcccgcc gtgtcgttct cccgaccgcg ccaccgctcg agcgtcttcc gcacgatcga
      181 gcgataccgc atcaccagtt ccttccacgc gtgatcgccc aacaccgcct gtttctccac
      241 cgacccgacg atgtcggtga acaggaccgt cgaaaggatg ctgtcgagtt ccggaggcgg
      301 gcgctggatg ccgcagaagt cgaacgtcgc ctcgatgacc gccggcgccc agagtcgaga
      361 cgccctcgca ggcatcgatc tgcagaccgc gcccgggatc aacgccgcgg tcgcccgggc
      421 ctgctcgacg acccactccc ctggataccc ctccgtccct agggccagga ccgggatccg
      481 gagcgtcggg aggatctcgc ggacgtcgta gtcgtgcagc aatcggatga acttgacggc
      541 ggtcgcagga ccacacgcgt gccgctgcat cctcgcgagg aagcggatga cgtcgtcgtc
      601 catctcggcg tgcgcgtaca tccggcgcac gaagtcctcg gttccccagg cgcgctccgc
      661 ctctgcagcg agcagatcca cgtcatccgg gtcgcgaccg aacggccacc cgcgcgtctt
      721 caccgatcgc gcctgcgcga agaaccagac gagcccggcg acccgatcgg ggtgcgtcgc
      781 ggcaaggagg gcggcaacca tgcccccatc ggccgacgcc agcagtatcg gacgacgtag
      841 acccgccgcg tcgaggaccg cgagaaggtc ggccgcccgc gtctccaggt ccgggagacc
      901 gcccatgtca tccgagaggc ccgtccctcg ccggtcgtgg agactcaacc gggagaagga
      961 cgcaagctcc aaccaagacc tcgcggcggg ctcgaagtcg aagaacagct ccacgttggc
     1021 cgcgatggag ccgatcacgg cgacctcgtt ctcgctcgag ggatcgccga gcagtttgta
     1081 cgcgatgtag ccgccgtcca gcgtcttcgc gtaccgggtc tgggcgacct cagtcacggg
     1141 tacccccacc gggtcgacgt gccagcggcc ggggaagctc ggttcctgtt cgccgaggtc
     1201 gaaggctcag tgggatatga gctcaccggg ccctcccagc cccggatgat cgccttcagt
     1261 cgctacccat accacggatg gagaacgccg tcttcgccgt gaccgggcgt cgagccgcat
     1321 gggagttccc cggcttcgcg tcgagggagc aaggcgctgg gtcccatccg agacccgaac
     1381 ccaggcttga tccgcggcgg aggaacttct catcacctga gcggaagcgc acacttcccg
     1441 tgatggtcga gacggctccg ccgagcccga gcggccggcg tgcgcgcgac ctcgccgccg
     1501 ccacacccgg gacgaggaac cgctacgccg acctcctccg tgtcgcctcg atcttcgtcg
     1561 tggtcagcgg ccactggctc atggcggtcc tcgagtacga ggacgggtcg ttcgtcggga
     1621 agaacctgct cgagatcgcg acctggaccc acatcctcac gtggatcttc caggtcatgc
     1681 cgatcttctt catcgtcggc ggcttcacca acgcgggctc gtggcgttcg gcgacccggc
     1741 gcggcgtgtc gtacgcgacc tggctgcgtg cgcgttcggc ccggttactc ggacccgccc
     1801 tcgtgttcgt cgccttctgg acagtcctgc cgatgctcac cgtcctcgtg ggtctcccgt
     1861 ccgggatggc gcgaaccggc gggcgggagg tggcgctccc gatctggttc ctggccgtct
     1921 acctgctgac ggtcgcggcg gccccgccgc tgctggcggc gcatcagcgg tacggcgtgc
     1981 gtgtgcttgt cgcgctcgcg gtaggagcgg caaccgtcga cgcggtgcgg tacggactcg
     2041 acgtcggatg gatcggcgtc gtgaactacg ccttcgtctg gctcgccatc ctggagctcg
     2101 ggttcctgtg gcgcgagggc gcgctcactc gcgtccggtg gatcccgtgg gccatggccg
     2161 gcacggggct cgcggccctg gcgttgctca ccaccgtgtt cgactacccg gtctcgatga
     2221 tcggcctcag tcacgcccag cgctcgaaca cgctcccgcc gacgaccgcg ctcctcgcgc
     2281 tcgccgtctg gcagtgcggc gcgatgctga tcttcgagaa gcccgcgaac cggtggctcc
     2341 tgcggccgcg cctatggctg ggcatcgtgg tcgccaacag catggtgatg acgttctacc
     2401 tgtggaacat gacggcggcg gtcctggccg cggtcatcct gctgcccacc gggatcgcgc
     2461 cgcagttcga ggagctcagt ggcgcgtggt ggctgtggcg gctcgcctgg atcgcggcgt
     2521 gcgcgatctg cctcgtgccg ctcctgttcg cgttccggtg ggccgaacgg cccggcgcgc
     2581 cgccacctcc ggcgccggcg ggatggagcg gcctcgtgat gtcgctcgcc ggcgtggcgt
     2641 gcgcctctgc cgggatgtcg atcatcgccg cggcggcgtt ccccgtgcag ggcgaggtgc
     2701 tggccatgcc cctgctcgga gtggcgttcc tggtcgtcgg agcgatcctg ctccacgtga
     2761 atccgttcag ccctctctcg aagcgctctg cccagagcct cctcggctga cgctccgatg
     2821 cccgacgggg cgggtaaggt cgcgatggca ccgacgaggt gaggtgaggc gcgatgcttg
     2881 gatgggtcga agacatcgag cgcgcgacgc tcgagaacga gaacttccgc accgtcctcc
     2941 acacgggcga gcacgtgcag ctgacggtca tgcagctgca gccgggcgag gacatcggcc
     3001 gcgaacgcca cccgcatacc gaccagttcc tcaggatcga gtcggggacc gggcgggtcg
     3061 agttcggtcg gaacgaggac gcggtggatg agacgcacga cgtcgaggag gactgggcga
     3121 tcgtcgtgcc ggcgggcgtg tggcacaacg tggtgaacac cggcgacggc gacctgaagc
     3181 tgtactcgct gtacgcgccg ccggagcacc cggacggcac ggttcaccgc acgaaggcgg
     3241 acgcggcggc ggccgagcac gactgatccg cccgccgccg gtttgccagg tggcccgggt
     3301 tggtaacgaa gcggaggaac cgaaaggagc gaacgcatga acctgctcgg ctacctgaac
     3361 gaccacctgg ccggagccgc cgccgggatc cagctcgcgg agcggtgccg ggatcgagat
     3421 cccgagagcg agctgggcca gatgctgcag ggcctcctgc tcgagatccg gcacgaccgg
     3481 gacgtgctcg aacgcgtgat ccgcgtcctg ggagggtcgc cggacacgat caagcgcggg
     3541 gcggcgctcg gggccgagcg gatctcgagc cttcggatgt ggctgccgat ggtcggtccc
     3601 ggctcgcggg catcggcgcg gctcgaggag atcgaggtcc tcagcctcgg catcgagggc
     3661 aagcggctgc tgtgggccgc gctcgcgcag ctgtcatcgg cggacgagcg gctgagcggc
     3721 ttcgcgttcc cggacctcaa acgccgggcg aggtcgcagc gggatcgcct ggagcgcttc
     3781 cggctccggc tcgccgcgga agccttcggt ccgccgaagg tggctccgat gcgggcgacc
     3841 gcctaacgga cacgcggacg ggacgccgag atcgagcgcc cgatcccacg tggcggggga
     3901 cccttgtccc ctcagggatc caccagccac cgttcgatcg acgacgacgc cagcagctgc
     3961 tcccgaacga acgggtcagc cgcgatcagt cgttccgctt cctcccgcga atcggcctcg
     4021 aagctgatgg ggccgcccga tcgaacctca tcgtcacacc tttctcggca ccttgccggt
     4081 cgccgagaac acgacccgca tcaccttgtg accggcgtcg gccgtgggtt cgaacatcag
     4141 cgggcatcct ccgtcgatca ccgtgacgcc atgctgcctg ccgtactcgg tcgcggcgtc
     4201 ggagacgctg cctcccccga acgagcgatg catccagaca cggtcgatcc cgagatccac
     4261 gcattcccgc atcgtgtcct cggcgatctc cggcctcgt
//
LOCUS       DATBRK010000072         8373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1451750, whole genome shotgun sequence.
ACCESSION   DATBRK010000072 DATBRK010000000
VERSION     DATBRK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451750"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..668
                     /gene="def"
                     /locus_tag="VIH70_02215"
     CDS             171..668
                     /gene="def"
                     /locus_tag="VIH70_02215"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY8115210.1"
                     /translation="MLRIRTLGDPVLRETSSPVEVFDDALRRIVDDMLETMYAAPGVG
                     LAGNQVGLRTACFVYDDREGHAGFVANPRLSEPEGEETLEEGCLSIPGPYHPTVRPAR
                     VRLDGVDLDGEPLDIVAEGLLARIFQHETDHLNGMLYIDRLDDAGRRDVMRQLREFEV
                     QRSEP"
     gene            716..1651
                     /gene="fmt"
                     /locus_tag="VIH70_02220"
     CDS             716..1651
                     /gene="fmt"
                     /locus_tag="VIH70_02220"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY8115211.1"
                     /translation="MIRSLRVVFLGNDRWSVPSLRAIADSAHEVVAVLTAARRPAGRG
                     NELRATPVADAAAEAGLSLHEVETVTSGPGAEALAASDPDLLIVVAYGEILPQGVLDL
                     PALAPVNLHFSLLPELRGASPVQTALLEGREETGVTTIVMDAGIDTGPVILRRPEAIE
                     GGDDAGSLGDRLAAIGAEILVETADLFAAGAAEPRPQDEAAATYTRRLGADDRVLRWS
                     EPARDLVNRTRALSPDPGATTRFRGQDLKIFRAQAVPAAGAPGSIVDAGRVGLVVATG
                     EDGFRPIELAPAGRRRMSWSDFLNGYRPVIGESLG"
     gene            1648..3018
                     /gene="rsmB"
                     /locus_tag="VIH70_02225"
     CDS             1648..3018
                     /gene="rsmB"
                     /locus_tag="VIH70_02225"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEY8115212.1"
                     /translation="MNGPVDRRRAGSTRRVGAPGRPTPAPSARSVALDVIRRVSDEGA
                     YSNLALARTLARVGLSERDAAFATELVYGTLRSLIPIDHWLAPLLDRPIDTAPKGARA
                     ALRLGAYQLRFLRVPVHAAVSETVALADPRHRGFVNAVLRRFATLEPQVPEGGTDVAV
                     SLRTGLAEWAVRELRRIVSSDEETERAAAALAARAPLTIRTNTCRTTVDELERELHAA
                     GVETERGAVHPDSLEIGSGAPSALPGFAQGWFAVQDQASSFVVAALDPRPGDRVLDVC
                     AGPGGKTGHLACVIGPEGRSVAADVSPARLELVRANVERLGVRTELLVQDGRRPAVRG
                     GFDRVLVDAPCSGIGSSRRRPELLWRARKDELSSLARLQVGVATAAAGLLCAGGRLVY
                     SVCTFPRAETDAACDAILRRVPELEPIEIAGPDGPAERVRLWPHVHGCDAMFVAAFRW
                     RATPRG"
     gene            3028..3687
                     /gene="rpe"
                     /locus_tag="VIH70_02230"
     CDS             3028..3687
                     /gene="rpe"
                     /locus_tag="VIH70_02230"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY8115213.1"
                     /translation="MAKLGASILSADLARLAEEVKLVGEHADLIHIDIMDGHFVPPLT
                     VGPAVVAALRPHTDRPLHAHLQVEAPDGFLDELAGAGADAVSFHLEAVSDPAPTQRRV
                     RGLGMRAGMALAPRTPAEAAFAFLEDADDVVVLAVEPGWAGQPFQPGAVAKIEALRSA
                     IDRRGLDVEVHVDGGVNLETVARCVQAGADVLVAATAIFGAGDPVRSALELRASAEAT
                     V"
     gene            3687..4619
                     /locus_tag="VIH70_02235"
     CDS             3687..4619
                     /locus_tag="VIH70_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8115214.1"
                     /translation="MPESILVVDDDPDIARFVEVNLRSAGYEVSVASDGEEALEKAAG
                     MRPDLVLLDVMMPRIDGFEVAHRLRRNPHTSNTSIIMLTAKALATDKVLGLTAGADDY
                     IIKPFDPIELLARVKGTLRRAKEMRNLSPLTGLPGNIRIQEEIERMIREESPFAVLYA
                     DLDNFKAYNDQKGFVQGDRLIQETARILQDAVAGRAGADGFVGHVGGDDFVAVVPPDV
                     AEDVAGLVIETFDQQIDGFYDPEDVQRGYVEVEDRKGKLQRLPLAGISIGIATTKVRS
                     FAHYGEAVSVATEMKQYAKREPKSSYAVDRRKVD"
     regulatory      4702..4830
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4897..5994
                     /gene="ribD"
                     /locus_tag="VIH70_02240"
     CDS             4897..5994
                     /gene="ribD"
                     /locus_tag="VIH70_02240"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY8115215.1"
                     /translation="MSDEAYMERAIELARRGSGLVSPNPMVGAVVVSDGAIVGEGSHE
                     GPGLPHAEVVALDRAGERARGATLFTSLEPCDHYGRTPPCTAAIVRAGIARVVSAARD
                     PNPVVDGQGVRSLRASGVEVLEGIREAEAQRLNAGFAKHVRTGMPFVIWKAAASLDGK
                     VAARDGSSRWITGEAARADVHRLRAWADAIVVGAGTALADDPELTVRDPDYRGRPPIR
                     VLVDARGRVGPSGDLFDDQAPTLVATTELAPTNARDAWREAGAEVLVVETDGEGVGLT
                     ALMRDLGKRDVQGVLLEGGPNLAWSAVEEGLVDRIVLYLSPKLLGGRDAPTVLDGRGF
                     APVGQALAIRIGSIERVGEDLRVEADVHGDR"
     gene            5975..6583
                     /locus_tag="VIH70_02245"
     CDS             5975..6583
                     /locus_tag="VIH70_02245"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY8115216.1"
                     /translation="MFTGIVEERGVVREVGTTRLSIGCRTVTSDATEGASIAVNGACL
                     TVVERSDEHLAFDVSEETLRRTSFSRLRAGDPVNLERPLTLSSRLGGHLVQGHVDGVG
                     EVVAIDPAEDGSAWITVRAPEPVARYLVDKGSVCVDGVSLTVASRDGETFGVALIPHT
                     LEVTTLGTAEVGDPLNLEVDVIAKYVEALIDPIEGQMEGNAP"
     gene            6580..7821
                     /locus_tag="VIH70_02250"
     CDS             6580..7821
                     /locus_tag="VIH70_02250"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY8115217.1"
                     /translation="MTQETGPVFATIDEALADIRAGKIVIVVDDADRENEGDFIMAAE
                     KATPESLNFMVTHGRGIVCMPMTAQRLDELRIPLMVSKNNESHGTAFAVSIDIEGRTT
                     TGTSAFDRAATVRAITDPSLQPGDIRMPGHVFPLMAQQGGVLKRAGHTEATVDLARLA
                     GLYPAGVLCEVLHPDGSMARAPELARVAREHDLKIISIADLIEYRRHREHLVTRTASA
                     QIPTEHGEFTAHSYESSVDGMVHVALVLGEVGDGEEILVRVHSECLTGDVFGSMRCDC
                     GDQLHGALAQVGKEGRGVVLYIRGHEGRAIGLTHKLRAYRLQEEGRDTVEANIELGFA
                     ADPRDYGIGAQILVDLGVRSVRLLTNNPAKRAGLEGYGLSISERVPLETRPTAENIGY
                     LRTKREKLGHLLENLEPEAAP"
     gene            7840..8319
                     /gene="ribH"
                     /locus_tag="VIH70_02255"
     CDS             7840..8319
                     /gene="ribH"
                     /locus_tag="VIH70_02255"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY8115218.1"
                     /translation="MSELRGDLLARGRRFAIAVARFNGLVTRKLLEGALAGLRANGVA
                     EEDVDVVWVPGAFELPLVARRLAASRTYDAVICLGAVIRGETAHFDLVAGQSAAGIRE
                     AAEATGVPVTFGVLTTETLEQALDRSGGKHGNKGWDAATAAIEMASLLEQLPKEAEP"
BASE COUNT         1181 a         2877 c         3134 g         1181 t
ORIGIN      
        1 tcatcgaacg tcggtggggc gacgatatgc ttggtcgcgc tcgatgcggc ccgcgtcccc
       61 ggtttcggcg ccgagatgcg ccggctcgcg gcggcgggga tcgtcacccg ggtcgaggcc
      121 gagccgcacc tgtgagcggc gaccgcgtag gtgggggaga catcgtgacc atgttgcgga
      181 tcaggacgct cggcgacccc gtgctgcggg agacgtcctc gcccgtcgag gtgttcgatg
      241 acgcgctccg ccggatcgtc gacgacatgc tcgagacgat gtacgcggct cccggcgtcg
      301 gtctcgccgg gaaccaggtg ggtctgcgga cggcgtgctt cgtctacgac gatcgcgagg
      361 gacacgccgg cttcgtcgcg aacccccggc tctccgaacc ggagggcgag gagacgctcg
      421 aggagggatg cctgtcgatc ccgggcccgt accacccgac ggtccgcccc gcgcgggtcc
      481 gcctggacgg cgtcgatctg gacggcgaac cgctcgacat cgtcgcggag ggtctcctgg
      541 cgcggatctt ccaacacgag accgatcacc tgaatgggat gctctacatc gaccgtctcg
      601 acgacgcggg ccggcgtgac gtgatgcgcc agctccgcga gttcgaggtg cagcgctcgg
      661 agccctgagc ggtcgacctc cgggagagca cgggggcgcg cgtatcctcg cacccgtgat
      721 ccgctcgctc cgcgtcgtgt tcctcggcaa cgaccgatgg tcggtgccct cgttgcgagc
      781 gatcgccgac tccgcgcacg aggtcgtcgc cgtgttgacg gccgcgcgca ggcccgccgg
      841 tcgcggcaac gagctgcgcg cgacgccggt cgcggacgcc gcggcggagg ccgggctgtc
      901 gctgcacgag gtcgagaccg tcacgtcggg cccgggcgcg gaagcgctcg ccgcgagcga
      961 ccccgacctg ctgatcgtgg tcgcctacgg cgagatcctg ccgcagggag tgctcgacct
     1021 cccggccctc gcgcccgtga acctgcactt ctcgctactg cccgagctcc ggggcgcgag
     1081 cccggtgcag acggcgctgc tcgagggacg cgaggagacc ggcgtcacga cgatcgtgat
     1141 ggacgccggg atcgacaccg gtccggtgat cctccgccgg cccgaggcga tcgagggcgg
     1201 ggacgacgcg ggttcgctcg gggaccggct cgcggcgatc ggggcggaga tcctggtcga
     1261 gaccgccgac ctgttcgcgg cgggcgcggc cgagccccgt ccgcaggacg aggcggccgc
     1321 cacgtacacc aggcgcctgg gcgcggacga ccgcgtcctg cggtggtccg aacccgcgcg
     1381 cgatctcgtc aaccgcaccc gggcgttgtc gcccgatccg ggcgcgacca cgaggttccg
     1441 cggccaggac ctgaagatct tccgcgcgca agccgtcccc gcggccgggg cgcccggctc
     1501 gatcgtcgac gccggccggg tgggcctcgt cgtcgcgacg ggggaggacg ggttccggcc
     1561 gatcgagctc gcgccggcgg gccggcggcg catgagctgg tcggacttcc tgaacggcta
     1621 ccgccccgtg atcggcgagt cgctcgggtg aacggtcccg ttgaccggag gcgcgccggg
     1681 tcgacgcgca gggtcggtgc cccggggcgg ccgacgccgg cgccgtccgc gcgttcggtc
     1741 gcgctcgacg tgatccgacg cgtcagcgac gagggcgcct actccaacct cgccctcgcg
     1801 cggacgctcg cgcgggtggg tctgtccgaa cgcgacgcgg cgttcgcgac cgagctcgtc
     1861 tacggcacgt tgcgcagcct gatcccgatc gaccactggc tcgcgccgct gctcgaccgt
     1921 ccgatcgaca ccgcccccaa gggtgcccgc gcggcgctgc gcctgggtgc ctaccaactg
     1981 cgcttccttc gtgtccccgt ccacgccgcg gtgtcggaga ccgtcgcgct cgccgacccg
     2041 cgccaccgag gcttcgtgaa tgcagtcctg cgccggttcg cgacgctcga gccgcaggtg
     2101 cccgagggtg ggaccgatgt cgcggtctcg ctgcggaccg ggctcgccga gtgggccgtg
     2161 cgcgagctcc gccggatcgt gtcgtcggac gaggagacag agcgcgccgc cgcggcgctc
     2221 gccgcacggg cgccgctcac gatccgaacg aacacctgtc gtacgacggt cgacgagctc
     2281 gaacgcgagc tgcacgccgc cggcgtcgag accgaacgag gggcggtgca tccggactcg
     2341 ctcgagatcg gctcgggtgc gccctcggcg ctgcccggat tcgcccaagg atggttcgcg
     2401 gtccaggacc aggcctcctc gttcgtcgtg gcggccctgg atccccggcc gggcgaccgg
     2461 gtcttggacg tgtgcgccgg tccgggcggc aagaccggtc atctcgcctg cgtgatcggg
     2521 cccgaggggc gctcggtcgc cgcggatgta tcgcccgcgc ggctcgagct cgtgcgcgcg
     2581 aacgtggagc ggctcggcgt tcggacggag ctgctcgtgc aggacggtcg ccggcccgcc
     2641 gtccggggcg ggttcgaccg cgttcttgtg gacgcgccgt gctcggggat cggctcctcc
     2701 cgccggcgcc ccgagctcct gtggcgggcc cgcaaggacg agctctcgag cctcgcccgg
     2761 ctccaggtcg gggtcgcgac cgccgccgcg gggctgctgt gcgccggggg ccggctcgtc
     2821 tacagcgtct gcacgttccc gcgcgccgag accgatgccg cgtgcgacgc gatcctccgg
     2881 cgcgtgcccg agctcgagcc catcgagatc gccggccccg acggccccgc cgaacgggtc
     2941 cgcctgtggc cgcacgtgca cggatgcgac gcgatgttcg tcgcggcctt ccgctggcga
     3001 gcaacccctc gaggctagaa tcgcgccgtg gccaagctgg gcgcgtccat cctttcggcg
     3061 gacctcgccc gtctggccga ggaggtcaag ctggtgggtg agcatgccga cctcatccat
     3121 atcgacatca tggacggaca tttcgtgccg ccgctcacgg tcggtccggc ggtggtggcc
     3181 gcgctgcgcc cccacacgga tcggccgctt cacgcgcatc tccaggtcga ggcgcccgat
     3241 gggttcctcg acgagctcgc gggggcgggg gccgatgccg tgagcttcca cctcgaggcc
     3301 gtgagcgacc cagcgcccac ccagcgaagg gtccgcgggt tggggatgcg ggccgggatg
     3361 gccctcgcgc cgcggacgcc cgcggaggcc gcgttcgcgt tcctcgagga cgcggacgat
     3421 gtggtcgtcc tcgcggtcga acccggatgg gcggggcaac cgttccagcc cggggccgtc
     3481 gccaagatcg aggcgctccg ttccgcgatc gatcgtcgcg gcctcgacgt cgaggtgcac
     3541 gtggacggcg gggtgaacct cgagacggtc gcccgatgcg tgcaggcggg cgccgacgtc
     3601 ctggtcgccg cgaccgcgat cttcggtgcc ggggacccgg tccgttcggc cctcgagctc
     3661 agagcgtcgg cggaggcgac ggtctgatgc cggagtcgat cctcgtcgtc gacgacgacc
     3721 cggacatcgc ccggttcgtc gaggtgaacc tccgcagcgc gggctacgag gtctcggtcg
     3781 cctcggacgg ggaggaggcg ctcgagaagg cggccggcat gcgccccgat ctcgtgctgc
     3841 tcgatgtgat gatgccgcgc atagacggtt tcgaggtcgc gcatcggctc cgccggaacc
     3901 cacacacctc gaacacgtcc atcatcatgc tcaccgcgaa ggcgctcgcg accgacaagg
     3961 tcctcgggct caccgcaggc gcggacgact acatcatcaa gccgttcgac ccgatcgagc
     4021 tcctcgcgcg ggtcaagggc acactccggc gagcgaagga gatgcgcaac ctctcgccgc
     4081 tcaccgggct gcccgggaac atccgtatcc aggaggagat cgaacggatg atccgcgagg
     4141 agtcgccgtt cgccgtgctg tacgcggacc tggacaactt caaggcctac aacgaccaga
     4201 aggggttcgt gcagggcgac cgcctgatcc aggagaccgc gaggatcctg caggacgccg
     4261 tggccgggcg tgccggcgcc gacgggttcg tcgggcacgt cgggggagac gacttcgtgg
     4321 cggtcgtgcc gcccgacgtc gccgaggatg tcgccggact cgtgatcgag accttcgacc
     4381 aacagatcga tgggttctac gaccccgaag acgtgcagcg cgggtacgtc gaggtcgagg
     4441 atcgcaaggg gaagctgcaa cggctgccgc tcgccggcat ctcgatcggc atcgcgacca
     4501 cgaaggtgcg ctcgttcgcg cattacggcg aggcggtctc ggtggccacc gagatgaagc
     4561 agtacgccaa acgcgagccc aagtcctcct acgcggtcga ccgccgcaag gtcgactgac
     4621 ccgccctccg tccggcttcg ccacgcctcg tgtccgcatg acctgcccgc gcgggcggcc
     4681 tatacttggg cttccacaag agcctgtctt cggggcgggg tgcaagtccc caccggcggt
     4741 gagagtccgc gaccccccga acgccattcg ggggttgacc cggtgagatt ccgggaccga
     4801 ccgtcaaagt cgggatggga gaggacgggc ggaacggcgg ccgacggctg ccccgtcctg
     4861 ctctctctgc atcgccgcct cgagatcgga agcgacatgt ccgacgaggc gtatatggag
     4921 cgggcgatcg agctcgcgcg gcggggcagc ggcctggtgt cccccaaccc catggtgggc
     4981 gccgtggtgg tctccgacgg cgcgatcgtc ggcgagggct cccacgaagg cccgggccta
     5041 ccgcatgccg aggtcgtggc gctcgaccgg gcgggcgagc gcgctcgggg cgccacgctc
     5101 ttcacctcgc tcgaaccctg cgaccactac ggccgcacac cgccgtgcac ggcggcgatc
     5161 gtgcgcgccg gtatcgcgcg ggtggtctcc gcggcccggg acccgaaccc ggtcgtggac
     5221 gggcaggggg tgcggagcct ccgcgccagt ggcgtcgagg tcctcgaggg gatccgcgag
     5281 gccgaggcgc agcggctcaa tgcggggttc gccaagcacg ttcgcaccgg gatgccgttc
     5341 gtgatctgga aggccgccgc gtcgctggac ggcaaggtgg cggcgcgcga cggctcctcg
     5401 cggtggataa ccggcgaggc cgcgcgggcc gacgtgcatc ggctccgagc gtgggcggac
     5461 gcgatcgtgg tcggtgctgg cacggcgctc gcggacgacc ccgagctcac ggtccgcgac
     5521 cccgactacc gagggagacc gccgatccgg gtcctggtgg acgcgcgggg gcgcgtgggt
     5581 ccgtcggggg acctgttcga cgaccaggcg cccaccctcg tcgcgacgac cgagctcgcg
     5641 ccgacgaatg cgcgtgacgc atggcgggag gcgggcgccg aggtcctggt ggtggagacg
     5701 gacggcgagg gggttgggct caccgcgctc atgcgcgatc tcggcaagcg cgacgtccag
     5761 ggggtgctcc tggagggcgg cccgaacctg gcctggtcgg cggtcgagga ggggctggtc
     5821 gacaggatcg tcctgtacct ctcgccaaag ctgctcggcg ggcgggacgc cccgaccgtc
     5881 ctggacggac gtgggttcgc gccagtaggg caggcgctgg cgatacggat cgggtcgatc
     5941 gagcgggtcg gcgaggacct gagggtggag gcggatgttc acggggatcg ttgaggagcg
     6001 cggcgtggtc cgcgaggtgg ggacgacgcg tctgtccatc ggctgccgaa ccgtcacctc
     6061 cgacgcgacc gagggtgcgt cgatcgcggt caacggcgcc tgtctcacgg tcgtcgagcg
     6121 ttcggacgag catctcgcgt tcgacgtgtc cgaggagact ctccggcgaa cgagcttctc
     6181 gcgcctgcgg gcgggcgacc ccgtgaacct cgagcggccg ctcacgctgt cgagcagact
     6241 cggcggtcac ctcgtccagg gacacgtcga tggcgtgggc gaggtcgtcg cgatcgatcc
     6301 cgccgaggac gggagcgcgt ggatcaccgt tcgcgccccc gaaccggtcg cgcgctacct
     6361 ggtggacaag ggctcggtgt gcgtcgatgg ggtcagcctg acggtcgcct cacgggacgg
     6421 ggagacgttc ggcgtggcgc tgatcccgca cacgctggag gtgacgacgc tcggcaccgc
     6481 cgaggtggga gatccattga acctggaggt cgacgtgatc gcgaagtacg tggaagcgct
     6541 catcgacccg atcgagggcc agatggaggg aaatgcgcca tgacacagga gaccggaccc
     6601 gtgttcgcga cgatcgacga ggccctcgcc gacatccgcg ccgggaagat cgtgatcgtc
     6661 gtggacgacg ccgatcgcga gaacgagggc gacttcatca tggccgcgga gaaggcgacg
     6721 ccggagagcc tgaacttcat ggtcacgcac ggccggggca tcgtgtgcat gccgatgacg
     6781 gcgcaacgcc tcgacgagct ccggatccca ctgatggtct cgaagaacaa cgagagccac
     6841 gggaccgcgt tcgcggtctc gatcgacatc gaggggcgga cgacgaccgg gacgagcgcg
     6901 ttcgaccgcg cggccacggt gcgggcgatc accgaccctt ccctgcagcc gggggacatc
     6961 cggatgcccg gccacgtctt cccgctcatg gcgcagcagg ggggcgtcct caagcgggcc
     7021 ggccataccg aggcgaccgt cgacctggcg cggctcgccg gcctgtaccc ggccggggtg
     7081 ctgtgcgagg tcctgcaccc cgacggctcg atggcgcgag ctcccgagct cgcgcgggtg
     7141 gcgagggaac acgacctcaa gatcatctcg atcgcggatc tgatcgagta ccgccgtcat
     7201 cgcgagcatc tggtgacacg gacggcctcg gcgcagatcc cgaccgagca cggggagttc
     7261 accgcgcatt cgtacgagag ttccgtcgac gggatggtgc atgtcgcgct cgtgctgggc
     7321 gaggtgggcg acggcgagga gatcctggtg cgcgtgcact cggagtgcct gaccggcgac
     7381 gtgttcggct cgatgcggtg cgactgcggc gaccagctgc acggcgcgct tgcgcaggtc
     7441 ggcaaggagg gccgcggcgt ggtcctgtat atccggggcc acgaggggcg tgcgatcggg
     7501 ctgacgcaca agctccgcgc ctaccgcctc caggaggagg gacgggacac cgtcgaggcc
     7561 aacatcgagc tcgggttcgc ggccgacccg cgtgactacg ggatcggcgc ccagatcctg
     7621 gtggacctgg gcgtgcgctc ggtgcggctg ctcaccaaca accccgcgaa acgggccgga
     7681 ctcgagggct acgggctgtc gatctccgag cgcgtgccgc tcgagacgcg cccgaccgcg
     7741 gagaacatcg gctatctgcg caccaagcgc gagaagctcg ggcacctcct cgagaacctc
     7801 gagcccgagg ccgcgccgtg agcgggccgt gagcgggccg tgagcgagct ccggggtgac
     7861 ctgctcgcgc gcggccgccg cttcgcgatc gcggtggcca ggttcaacgg gctcgtcacg
     7921 cgcaagctgc tcgaaggcgc gctcgccggc ctccgcgcga acggggttgc cgaggaggac
     7981 gtcgacgtcg tgtgggtgcc gggagcgttc gagctacccc ttgtggcgcg gcgactcgcg
     8041 gcgtcgcgga cctacgacgc cgtgatctgc ctcggcgccg tgatccgggg cgagaccgca
     8101 catttcgatc tcgtcgcggg gcagtcggcg gccgggatcc gcgaggccgc cgaggcgacc
     8161 ggcgtcccgg tcaccttcgg ggtcctgacg acggagacgc tcgagcaggc gctggatcgc
     8221 tcggggggca aacacgggaa caagggatgg gacgcggcga cggcggcgat cgagatggcg
     8281 tcgctcctcg aacagctgcc caaggaggcc gaaccgtgat cgtcgacaag ccctggggca
     8341 aggtcgtgac gtacgcgctc aaccagccga gct
//
LOCUS       DATBRK010000073         7883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2174573, whole genome shotgun sequence.
ACCESSION   DATBRK010000073 DATBRK010000000
VERSION     DATBRK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174573"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VIH70_02260"
     CDS             complement(<1..823)
                     /locus_tag="VIH70_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115219.1"
                     /translation="MSGKRRRRPPRPRPAPAEAEPTETPAESAATPGEPRPGFGGALF
                     GRGGGSVMPPIGRSLGRGLLAVVSSPVLLAIAVVVPLLAWLALYALGFQGRPALLVDL
                     LALPPIATYFDLGTGESLLGLGPSFLIFTGVAILIRGVVYGLLAGMIVESLEDGRVSS
                     WGLLRGVAAIPTVIVVQVLSFTLILASNIIFPILGPGIGLLAAVGSLVGGLFFLGFAP
                     TAAVRQRRGVVETIRRSGRAAMLPGSRHLLFCGLYFFIALPVVVGLAPGGGEVTAN"
     gene            complement(820..2346)
                     /locus_tag="VIH70_02265"
     CDS             complement(820..2346)
                     /locus_tag="VIH70_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY8115220.1"
                     /translation="MRRDRSSRAVRLAAAAAVATAPVVVPSSAAVAREAPTWSQFQGG
                     PGHSGVLAGGPEPPYRVRWTLPAPAGDALSGAVVAGDLAVSIGNEAVYGIDVASGAIE
                     WRVARDGGPLSIPAIGVGPRGRILVYLEGPGPNDAGGAPDGSPSPSPSPSAGAASSAA
                     STTGPAPSPTGPGEQPDVSTLVAVALADRTELWRTPLEATSRSGVTIDGTNAFVGDQG
                     GTVYAVSLESGTISWSAEMDGRVDSAVAVADAQVVAVARNTDTAQVVVSAFDEATGER
                     SWPALAIQANSTAGTAPSAGGGSLFIGSADRRVRALDAENGTERWATLALSLFSPATS
                     LAFDDENVYAADIAGGLYRLDSADGGRRWSYHLNEVVLRSSPVVSGSSVLLGLGDGRL
                     VAVDAASGHLVWESAPSAGLIGTIALSSDAVIAVKGGRNAGLIAYEHDPQGALVDVSS
                     PTQLEGGTTLTRWVLAAVIVFAVAFVPGIWATRRLSGVADDEPGDGGSDRISDPGEGP
                     "
     gene            complement(2440..2961)
                     /locus_tag="VIH70_02270"
     CDS             complement(2440..2961)
                     /locus_tag="VIH70_02270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8115221.1"
                     /translation="MLMVTLTGPSSPSGGPLPIGSGADRIAFLPALQVGTWAWPVQGP
                     VIRGFDPPDTPFGAGHRGIDIAVAPGTPILACEAGTVSFAGRVGGELFVTIVHGGGLS
                     STYSWISSTTVGKGDVVSRGQPIGATGTGPPGASVPHLHLGAKLDGEYVDPLLLLAPL
                     GVEGLIRLVPILS"
     gene            complement(3076..4026)
                     /gene="whiG"
                     /locus_tag="VIH70_02275"
     CDS             complement(3076..4026)
                     /gene="whiG"
                     /locus_tag="VIH70_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEY8115222.1"
                     /translation="MPRAPKAEKAKTPVELPRPTKADRAETVKAAKNDRAATRSGPVR
                     VGSSSRVSNRQPPSPDELNELARLWEEFKQNEVSGAREGARERLILHYAPLVKYVASR
                     VATGLPASVDQADLVSYGMFGLIDALEKFDPGRGNKFETYAIPRIKGAIIDELRAMDW
                     VPRSVRFKAREIEKAYSDLESIHKRGPTEKEVAARLGVTLRELHEVINQISFVQVLQL
                     DEILSVGSDRGEQVSLLDTLADRGTDPTTALEGEETRGLLAAAINSLSEREKIVVTLY
                     YFEGLTLAEIGDILGVTESRVCQIHTKAVGQLRLQLVETE"
     gene            complement(4026..4952)
                     /locus_tag="VIH70_02280"
     CDS             complement(4026..4952)
                     /locus_tag="VIH70_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY8115223.1"
                     /translation="MPARDPAQTDVALIDGFADHLALERHLASSTVDAYRRDLSQLAA
                     FLRRFRRDLPGAKIDDLRRFLAQLTSLGYARASIARRVGAIHTFYRWAVSQEVVAEDP
                     AALLGRPKVVNRLPVVLRVPEAAALVEAPVVTGDARDVEVAVALRDRAILELMYGSGL
                     RVSEVAGVTIDRVDLERGRVTVVGKGDKEREVPLGDPAREAVAAWLAVRGVLGVGADD
                     ELFMNRRGRPIGPRDIRRLVGRYAGVALSGRRVTPHTLRHSFATHLLEGGADIRSVQE
                     MLGHASVATTQRYTHVSRARLFEAYERSHPRA"
     gene            complement(5108..6340)
                     /gene="dprA"
                     /locus_tag="VIH70_02285"
     CDS             complement(5108..6340)
                     /gene="dprA"
                     /locus_tag="VIH70_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY8115224.1"
                     /translation="MRSEDRPGGPWPTWPPGYGSDDEDRRALFVLSALRGISPRRLIP
                     LAVERGSAASTLAWIREGRGGSEKDQAFARALDPDAIAAAAEACGARLVTWASVDYPS
                     QLRHIPDPPGAIYVIGRTPPDVSSAVAMVGARRCTASGREAAHAIGRGLALAGLTVVS
                     GAARGIDAASHDGALSVRGPTLAVLGSGLNVPYPSASRALIRRIGSAGTLVSEYAPGT
                     PPSQHHFPARNRVVAGLCRATVVVEGARGSGSLITAEHAMEFGRDVYALAGSVTSALS
                     EVPLQLIREGATLIRDAEDLLGDLGAEVVPIEAAERVDLDDTERRVLAGIVESTLPDR
                     LASSLGMAIHEVVGILMRLELRGFVRNVGGRYETTLKARPRRDAANERGARGTPERAT
                     RPTGTPAPSSRLAAIDER"
     gene            complement(6330..7862)
                     /locus_tag="VIH70_02290"
     CDS             complement(6330..7862)
                     /locus_tag="VIH70_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY8115225.1"
                     /translation="MYGRVNGVTVVGVRGHLVAVEAHVGRGLPSLTLTGLPGAGVQDA
                     RDRIRPAVESAGLEWPLRRVVVNLSPGTLRKEGPGLDLPVAMGVLAATAQVPAVGLEG
                     WVFAAELSLRGGLVSTPGVLSVAIAAARAGLRGVIVPASNASEAALVDDLEVIGAGSL
                     GEVVGFLRGTWRPAAPEATGPRDDTPHAVDLAEVRGQAPARRALEVAAAGGHNVLMVG
                     PPGAGKTMLARRLPTILPTMSRDESLQVTQLHSVAGLLDGGLIHARPFRSPHHTVSPS
                     ALLGGGTAFLRPGEVSLAHHGVLFLDEFTEFRRDAIEGLRQPLEDGRVVVTRMVGSVV
                     FPASFTLVAAANPCPCGFAGDAVRRCTCREDRLQTYHDKLTGPLLDRVDIRLVIPRLT
                     KRELLGEQPGESSPAVRERVEQARARQRRRYARFGFPNNGQLPGPLARREARMSADAR
                     DLLASAVDLHALTGRGFDRTLKVSRTIADLAGADRVEPDHLVEALAYRSASPVPGLER
                     AV"
BASE COUNT         1145 a         2984 c         2744 g         1010 t
ORIGIN      
        1 ggttcgcggt gacctctccc ccacccgggg ccagaccgac gacgacgggc agtgcgatga
       61 agaagtacag gccgcagaac aggaggtgcc ggctccccgg caacatcgcg gccctgcccg
      121 aacggcggat ggtctcgacc acgcctcggc gttgccggac cgcggcggtc ggcgcgaacc
      181 cgaggaagaa caggcccccg acgagcgacc ccacggcggc gagcagtccg atcccgggcc
      241 cgaggatcgg gaagatgatg ttgctggcca ggatcagcgt gaacgagagc acctggacga
      301 cgatcacggt cgggatcgcc gcgacgcccc ggagcaggcc ccaggagctc acgcgaccgt
      361 cctcgagcga ctccacgatc atccccgcga ggaggccgta gaccacgccc cggatcagga
      421 tcgccacgcc ggtgaagatc aggaacgacg gtccgagccc caacaggctc tcccccgtcc
      481 ccagatcgaa gtacgtcgcg atcggcggca gtgcgaggag gtcgaccagc agggcgggtc
      541 ggccctggaa cccgagcgcg tacagggcca gccacgcgag cagcggcacg acgaccgcga
      601 tcgcaagcag gaccggcgag gacacgacgg cgagcaggcc acgcccgagc gagcgcccga
      661 tcggcggcat cacggaccca ccgccccgac cgaagagcgc gccgccgaac ccgggacgcg
      721 gttcgcccgg tgtcgcggcc gactcggccg gggtctcggt gggttcggcc tccgccggag
      781 ccggccgcgg ccgcggcggg cgccgccgcc tcttcccgct cacggtccct cccccgggtc
      841 cgagatccga tccgacccgc cgtcgccggg ctcatcgtcg gcgacccccg acaagcgccg
      901 cgtggcccag atgcctggaa cgaacgcgac cgcgaagacg atcacggccg cgagcaccca
      961 tcgcgtcagc gtggtcccgc cctcgagctg cgtcggcgac gagacgtcga ccaacgcacc
     1021 ctgagggtcg tgttcgtacg cgatcagccc ggcgttccgg cctcctttga ccgcgatgac
     1081 cgcgtcgctc gacagcgcga tcgtcccgat caatcccgcg gacggcgcgc tctcccacac
     1141 gagatgcccg gaggcggcgt ccaccgcgac caggcggccg tcgccgagcc ccagcaggac
     1201 cgaggacccg gagacgaccg gcgagctcct cagcaccacc tcgttcaggt ggtagctcca
     1261 ccggcgcccc ccgtccgccg aatccagccg atacaggccc ccggcgatgt ccgccgcgta
     1321 gacgttctcg tcgtcgaagg cgagcgaggt cgcgggggag aacagcgaga gcgccagcgt
     1381 cgcccacctc tcggtcccgt tctcggcgtc gagcgcccgc acgcgccggt cggccgaccc
     1441 gatgaacagg gagccgccgc cggccgaggg cgcggtgccg gccgtggagt tcgcctggat
     1501 ggccagcgca ggccacgagc gctcccccgt cgcctcgtcg aacgccgaga cgacgacctg
     1561 cgccgtgtcg gtgttccggg cgaccgcgac cacctgggca tcggcgacgg ccaccgccga
     1621 atcgacgcgc ccgtccatct ccgcggacca cgagatcgtg ccgctctcaa gcgagacggc
     1681 gtacaccgtc ccgccctggt cacccacgaa cgcgttcgtc ccgtcgatcg tgaccccgct
     1741 ccgcgaggtc gcctccaacg gcgttcgcca gagttcggtc cggtcggcga gcgcgaccgc
     1801 gaccagcgtc gagacgtcgg gctgctcgcc cggtccggtg ggagacgggg cgggccccgt
     1861 cgtcgaagcc gcgctcgagg cggcgccggc gctcgggctc ggactcgggc tcggcgagcc
     1921 atccggcgct ccgcccgcat cgttcggacc gggcccctcg aggtacacga ggatccggcc
     1981 ccgggggcct accccgatcg cggggatcga cagcggtccc ccatcccgag ccactcgcca
     2041 ctcgatggcg ccagaggcga cgtcgatccc gtagacggcc tcgtttccga tcgacacggc
     2101 caggtcgcct gcgaccactg cgcccgaaag cgcgtcgccc gcgggcgccg gcagggtcca
     2161 tcggactcgg taggggggct ccgggccgcc ggccagcacc ccggagtggc cgggtccgcc
     2221 ctggaactgc gaccaggtgg gcgcctcccg cgcgaccgcc gcgctcgacg gaaccacgac
     2281 cggcgcggtc gcgaccgccg cagcagcagc caggcggaca gcgcgcgacg atcgatcacg
     2341 gcgcatcggg aacagctggg ctctcctccg acgggggcgc ccatgctaac tgccgtcacc
     2401 ttcccgcgcc cgatgctccg tcgtccggac gcggtggggt caggacagga tcggcacgag
     2461 ccgtatcagg ccctccacgc cgagcggtgc caggagcagg agcgggtcca cgtactcccc
     2521 gtcgagcttc gcgccgaggt gcaggtgcgg gacgctcgcc cccggcggtc cggtcccggt
     2581 ggccccgatc ggttgccccc tcgagacgac gtcacccttg ccgaccgtcg tcgacgagat
     2641 ccacgagtac gtgctcgaga gcccgccgcc gtggacgatc gtgacgaaca gctcaccgcc
     2701 caccctgcca gcgaacgaga ccgtccccgc ctcgcaggcg aggatcggcg tcccgggggc
     2761 gaccgcgatg tcgatccccc ggtggccggc cccgaacggc gtgtcgggcg ggtcgaaccc
     2821 ccggatcacc ggcccctgca ccggccaggc ccacgtcccg acctgcagcg ccgggaggaa
     2881 cgcgatgcgg tcggccccgg agccgatcgg cagcggccca cccgagggcg acgacggacc
     2941 cgtgagggtc accatcagca tggggacgac aaacacccgg aacgccacgc ggttctccga
     3001 tcgacgaccg agcgacgatg ctaggcgggc ggagcgacgc caaccgtgac gcggttcaca
     3061 ctcgtggcga tcggttcact cggtctcgac gagctgcagg cggagctggc cgaccgcctt
     3121 ggtgtggatc tggcagaccc gcgattccgt cacgccgagg atgtcgccga tctcggccag
     3181 ggtgaggccc tcgaagtagt acagggtgac gacgatcttc tcgcgttcgg acagcgagtt
     3241 gatcgccgcc gcgagcagtc cgcgcgtctc ctcgccctcg agcgccgtcg tcgggtcggt
     3301 cccgcgatct gccagcgtgt ccagcagcga cacctgttcg ccgcggtcgc tcccgacgct
     3361 gaggatctcg tcgagctgga gcacctgcac gaacgagatc tggttgatga cctcgtggag
     3421 ctcgcgcagg gtcacgccca gacgcgcggc cacctccttc tcggtcgggc cgcgcttgtg
     3481 gatcgactcg aggtccgagt acgccttctc gatctcgcgc gccttgaacc gaacggatct
     3541 cggtacccag tccatcgcgc ggagctcgtc gatgatcgcg cccttgatcc tcgggatcgc
     3601 gtacgtctcg aacttgttgc cgcggcccgg gtcgaacttc tcgagggcgt cgatcagccc
     3661 gaacatcccg tacgagacga ggtcggcctg atccacgctc gcgggcaggc cggtcgccac
     3721 gcggctcgcg acgtatttca ccagcggggc gtagtgcagg atgaggcgtt cgcgagcgcc
     3781 ttcccgagcg cccgagacct cgttctgctt gaactcctcc cacagccggg cgagctcgtt
     3841 gagctcgtcg ggcgacgggg gctggcggtt ggagaccctc gacgacgacc ccaccctgac
     3901 cggtccggac cgcgtcgcgg cccggtcgtt cttggcggcc ttcaccgtct cggccctgtc
     3961 ggccttcgtc ggcctgggca gctccaccgg ggtcttggcc ttctcggcct tgggcgctcg
     4021 aggcatcagg ctctcgggtg gctccgctcg taggcctcga acaggcgcgc acgactcacg
     4081 tgcgtatacc gctgggtggt tgccacgctc gcgtggccga gcatctcctg gaccgacctg
     4141 atgtccgctc ctccctcgag caggtgcgtg gcgaacgagt gtcgcagggt gtgcggcgtc
     4201 acccgccggc ccgaaagggc tacgcccgcg tatcgtccca caaggcgtcg aatgtcgcgt
     4261 gggccgatcg gccgacccct gcggttcatg aacaactcgt cgtccgcgcc gacgcccagg
     4321 acgccacgca ccgcgagcca ggccgcgacc gcctcccgcg ccgggtcgcc gagcggcacc
     4381 tcgcgctcct tgtcgccctt gccgaccacc gtcacccggc cgcgttcgag atcgacgcgg
     4441 tcgatcgtga cccccgcgac ctcgctcacc cgtagccccg atccgtacat cagctcgagg
     4501 atcgcccggt cgcgcagcgc caccgccacc tcgacgtccc tcgcgtcccc ggtcacgacg
     4561 ggcgcctcga cgagcgcagc cgcctccggc acgcgcagga ccaccgggag gcggttcacg
     4621 accttcggcc tgccgaggag ggcagcggga tcctccgcga cgacctcctg cgagaccgcc
     4681 caccggtaga aggtgtggat cgcaccgacg cgccgtgcga tcgacgcgcg cgcgtacccg
     4741 aggctcgtga gctgggcgag gaacctgcgc aggtcgtcga tcttcgcccc cggcaggtct
     4801 cgccggaacc ggcgcaggaa cgcggccagc tgcgaaaggt ctcgtcggta ggcgtccacg
     4861 gtcgaggacg cgagatggcg ctcgagcgcg aggtgatccg cgaacccgtc gatcagggca
     4921 acgtcggtct gagcaggatc gcgggcgggc atggtcctcc gagctcggcg gtcttccctt
     4981 gtaccaaccg acgtccgccc gttcaagcag cgcatccgga tcggctcatt ggtgcgcgga
     5041 ccggccgtgg gtgcgatgga gctccgcgac acgggctgcg tgttcagtcg gtgatctggg
     5101 cgaccggcta ccgctcgtcg atggccgcga gcctgctcga cggcgcgggc gtcccggtag
     5161 ggcgcgtggc gcgttcgggc gttccccggg cgccgcgttc gttggctgcg tcgcgtcgag
     5221 gtcgtgcctt gagcgtcgtc tcgtagcggc cacccacgtt gcggacgaac ccgcgcagct
     5281 ccagccgcat caggatgccg acgacctcgt ggatcgccat cccgagcgag gaggcgaggc
     5341 ggtcgggcag ggtggattcg acgatcccgg ccaggaccct gcgctcggtg tcgtcgaggt
     5401 ccacgcgctc ggcggcctcg atcggtacga cctccgcgcc gaggtcgccc agcagatcct
     5461 cggcgtcgcg gatcagggtc gcgccctcgc ggatcagctg cagcggcacc tccgacaggg
     5521 cgctcgtgac cgaacccgcg agggcgtaca cgtctcgccc gaactccatc gcgtgctcgg
     5581 ccgtgatcaa cgagccgctc cccctcgctc cttcgaccac taccgtcgcg cggcacagcc
     5641 ccgcgacgac ccggttgcgc gcggggaagt ggtgctggct gggcggcgtt ccgggcgcgt
     5701 actcgctcac gagggttccc gcactgccga tccgccggat cagggcccgg ctggccgatg
     5761 gatacgggac gttcagcccc gacccgagaa cggcgagcgt cggtccccgc accgatagcg
     5821 cgccatcgtg tgacgcggcg tcgatgccgc gagcggcgcc gctcacgacg gtgagcccgg
     5881 cgagcgccag cccgcgcccg atcgcgtgag ccgcctcccg ccccgaggcg gtgcagcgcc
     5941 gtgcgccaac catcgcgacg gccgaggaca cgtccggcgg cgtccgaccg atcacgtaga
     6001 tcgcgccggg cggatcgggg atgtgccgga gctggctggg gtagtccacc gaggcccacg
     6061 tgaccagccg agccccgcac gcctccgccg cggcggcgat cgcgtcgggg tccagggcac
     6121 gtgcgaacgc ctggtccttc tcgctgccgc cccggccctc acggatccag gccagggtcg
     6181 aggccgccga gccgcgttcg accgccagcg ggatcagtct ccgtggcgag atcccacgaa
     6241 gcgccgacag gacgaacagc gctcgtcggt cctcgtcgtc cgagccgtaa cccggcggcc
     6301 acgtgggcca cgggccgccg ggtcggtcct cagaccgcac gctcgagacc cggtaccggc
     6361 gaggccgagc gatacgccag cgcctcgacg aggtgatcgg gttcgacgcg gtccgccccc
     6421 gcgaggtccg cgatcgtccg tgagaccttg agcgtccggt cgaacccgcg acccgtgagc
     6481 gcgtgcaggt cgaccgccga cgcgagcagg tcgcgggcgt ccgcggacat cctcgcctcg
     6541 cgccgcgcga ggggacctgg gagctgaccg ttgttcggga agccgaaccg cgcgtagcgc
     6601 cggcgctgcc gcgcgcgcgc ctgctcgaca cgctcgcgga ccgccggcga ggactccccc
     6661 ggctgttcgc cgaggagttc gcgcttggtg aggcgcggga tcaccagccg gatgtcgacg
     6721 cggtccaaca acgggccggt gagcttgtcg tggtacgtct gcagacggtc ctcccggcat
     6781 gtgcagcgtc ggacggcgtc ccccgcgaac ccacacgggc acgggttcgc cgcggcgacg
     6841 agcgtgaagc tcgccgggaa caccaccgag cccaccatcc gcgtgacgac cacgcgcccg
     6901 tcctcgagcg gctggcgcag gccctcgatc gcgtcgcggc ggaactccgt gaactcgtcg
     6961 aggaacagca ccccgtggtg cgccagcgac acctcgcccg gtcgcaggaa cgccgtcccc
     7021 ccgccgagca gggcggatgg cgacaccgtg tggtgcggcg aacggaacgg ccgagcatgg
     7081 atcaggcccc cgtcgaggag tccggccacg gaatgcagct gggtcacctg gagcgactcg
     7141 tcgcgcgaca tcgtcggcaa tatcgtgggg agtcggcggg cgagcatcgt cttgcctgca
     7201 ccgggtggac cgaccatgag cacgttgtgt ccgccggccg ccgccacctc gagcgcccgg
     7261 cgggccgggg cctgacctcg gacctcggcg agatcgaccg cgtgcggcgt gtcatcgcgc
     7321 gggcccgtcg cctcgggcgc ggccggccgc cacgtccctc gcaggaaccc gaccacctcc
     7381 ccgagcgatc ccgcgccgat gacctcgaga tcgtccacca gcgcggcctc ggacgcgttc
     7441 gacgccggga cgatcacgcc tcgcagcccc gcgcgcgccg cggcgatcgc caccgacagc
     7501 acgcccgggg tcgagaccag cccgccccgg agcgacagct cggcggcgaa cacccacccc
     7561 tcgagcccca ccgcgggcac ctgcgcggtc gccgcgagca cccccatcgc gaccggcagg
     7621 tcgaggccgg ggccctcctt gcggagcgtc cccggcgaga ggttcacgac gacccgacgg
     7681 agcggccatt ccaggcccgc gctctcgacc gccggtcgga tccggtcgcg cgcgtcctgc
     7741 acgcccgcgc cgggcagccc ggtcagggtg agcgacggga gtccgcgacc gacgtgcgcc
     7801 tcgacggcga cgagatggcc gcggacgccg acgacggtga cgccgttgac gcgtccgtac
     7861 acggtgggcc gaccctagcg gca
//
LOCUS       DATBRK010000074         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_442497, whole genome shotgun sequence.
ACCESSION   DATBRK010000074 DATBRK010000000
VERSION     DATBRK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442497"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /gene="steA"
                     /locus_tag="VIH70_02295"
     CDS             complement(<1..980)
                     /gene="steA"
                     /locus_tag="VIH70_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEY8115226.1"
                     /translation="MRRPKPQGGLARVDPRTKRLVQRVVPGEIAVIDHEDLDGVSAEA
                     LVTASVGGVVNAARSISGRYPNMGPIRLLEAGIPLVDTVGPLLLQKVREGDELRLDGD
                     RVYVGDRLVGVGIRQSSATIRESMGEAQLRLADRFESFARNTIDYMQRERDLLFGGSG
                     LPPLDHDLANRPVLVVVRGYNYKEDLAVLRPYIRDVRPVLLGVDGGADALIEAGYTPD
                     LILGDMDSVSDEALSFAGPARRRWLRRSRPTELVLHAYRDGRAPGRERLEALGVPFRV
                     VEAAGTSEDAAFLLAHEKGAETIVAVGSHGNLREFLDKGREGMSSTFLVRL"
     gene            complement(1165..2820)
                     /gene="recN"
                     /locus_tag="VIH70_02300"
     CDS             complement(1165..2820)
                     /gene="recN"
                     /locus_tag="VIH70_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY8115227.1"
                     /translation="MLRDLHISGLGVIDDLDLELHQGLNVLTGETGTGKTMVTVGLQL
                     ALGRRGGSSLVRVGAPAAKVQARFDAPSAAVSAGWAEDGEVLLARTVTAEGRSTARVG
                     GQLAPVSALAELARELVEIHGQHDGQRLLSTVAQTRFLDRYAGAEHLDAVDALRVEHG
                     RLRDARAALAELDERERDRERQMDLLAYQVREIEGASPRPGETEELKLEAARLDHAGR
                     LLERASSAERALAAEDGAADALGVAAAELRTAGEVDPGAEELAVRAFDLSAAATELAV
                     EVRTYRERLELDPERLHEVRERIDALDALQRKYGKGEDEVLAFLDRARESLEALAGVE
                     QERERAAARVAELNERVAALATAVSDGRATAAPELTRALRGELGELGMEGATIEVTLV
                     PNPELVSGGAEHAAFVFSGGPGQPAQPLAKVASGGELSRTMLGCRSVLVDLDDVPTLI
                     FDEVDAGIGGLAGVAVGRRLANLALVRQVVVVTHLPQIASFADRHIRVRKDGGTASIE
                     VLDDAQRVEELSRMLAGLPGSDAAAAHAEELLSEAGRTPRSSP"
     gene            complement(2830..3702)
                     /locus_tag="VIH70_02305"
     CDS             complement(2830..3702)
                     /locus_tag="VIH70_02305"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY8115228.1"
                     /translation="MTSSASGSRLRAAGFVVHPARRAAAEAARSIGGWLEDRAVQTRT
                     IAGDDAEDEEAARAFARGLDLVVSVGGDGTFLRAARVASVSGVPVLGVKVGRMGFLTE
                     VEPGQATAVLERMLDGSARIEERLAVVAEGSSFEPQWALNEVIVEKSARHRLIRLAVF
                     VDDSYVTTFSADGVISATPTGSTAYSFSAGGPIVSPSVPCIVVTPIAAHMVFDRSLVL
                     APDQRVRLEVLGEEPGLLSADGRHSLELPVGSTVQVERAPTPARFVRREDAPSFHDLV
                     RDKFDLPGSVGGAV"
     gene            complement(3699..>4537)
                     /locus_tag="VIH70_02310"
     CDS             complement(3699..>4537)
                     /locus_tag="VIH70_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY8115229.1"
                     /translation="SKRAGLTLRAVARRRLDAELVRRGLVATRSQAQSAVRDGLVLVS
                     GRSASKPSTLVDAAEPLELVGEPRRFASRAGVKLEAALERFAIDVHGRDGLDAGASTG
                     GFTDCLLRRGAAHVVAVDVGYGQLAWSLRTDRRVTVLDRTNVRDLRPEDLPYAAGIVV
                     ADLSFMSLRLAIPALARVSSSDADLVLLVKPQFEVGRGRVGSGGVVRDPDAWRDAILG
                     VADAVSEHGLSPIAVMASPLPGPAGNIEFPLHARVGSARTVVRDVEGALEEARAIAEV
                     PS"
BASE COUNT          629 a         1721 c         1609 g          578 t
ORIGIN      
        1 agccggacca ggaaggtgct cgacatccct tcccggccct tgtcgaggaa ctcgcggagg
       61 ttgccgtggc tgccgaccgc cacgatcgtc tcggcgccct tctcgtgggc gagcaggaac
      121 gcggcgtcct cgctggtgcc ggccgcctcg accacgcgga aaggcacgcc gagcgcctcg
      181 agccgttccc gacccggtgc gcgtccgtcg cggtacgcgt gcaggaccag ctccgtgggc
      241 cggctgcgcc ggagccaccg tcgccgcgca gggccggcga acgagagcgc ctcgtccgag
      301 acactgtcca tgtcccccag gatcaggtcg ggcgtgtacc cggcctcgat cagggcgtcg
      361 gcgccgccgt cgaccccgag caagacgggt cgcacgtcgc ggatgtacgg acgcaggacg
      421 gcgagatcct ccttgtagtt gtagccacgg accacgacca gcaccggacg gttcgcgagg
      481 tcatggtcga gcggcggcag accggagccg ccgaacagca ggtcgcgctc gcgctgcatg
      541 tagtcgatcg tgttgcgcgc gaacgactcg aaccggtcgg caagccggag ctgcgcctcc
      601 cccatcgact cgcggatcgt ggcagagctc tgccggatcc ccacccctac cagtcggtcg
      661 ccgacgtaga cccgatcccc gtcgaggcga agctcgtcgc cctcgcggac cttttgaagg
      721 agcagcgggc cgaccgtgtc gaccagcggg atgcccgcct cgagcaggcg gatcggcccc
      781 atgttcgggt accggccgct gatcgaacgg gccgcgttca ccacgccgcc gaccgacgcg
      841 gtgaccagcg cttcggccga gaccccgtcg aggtcctcgt ggtcgatcac cgcgatctcc
      901 cccggcacga cgcgctggac cagccgcttg gtcctcgggt cgacgcgagc gagaccgccc
      961 tggggcttcg gccgccgcaa tggccgccgg agcaggctcc tcggcttggt tccgaacgtg
     1021 gacgcctcct tggtccctcg accgctcggc ccgacctcgc cggcgggcgt tcaggccggt
     1081 ggagccggtg accaggggcg gctcctcgtg cgggaagggt atcggagtct tcgatggtgg
     1141 gcccgggtga tcccggaccg ggcgttacgg ggacgacctg ggcgtgcggc cggcctcgga
     1201 cagcagctcc tcggcgtgag cggcggcggc gtcgctcccg ggcagtccgg cgagcatcct
     1261 cgagagctcc tcgaccctct gggcgtcgtc gagcacctcg atcgaggccg tcccgccgtc
     1321 cttgcggacg cggatgtgcc ggtccgcgaa cgacgcgatc tgcggcaggt gggtcaccac
     1381 gacgacctgc ctcacgagcg cgaggttcgc caggcgccgc ccgacggcga ccccggccaa
     1441 gccgccgata cccgcgtcga cctcgtcgaa gatcaacgtc ggcacgtcgt ccaggtcgac
     1501 gagcacgctc ctgcatccga gcatcgtgcg cgagagctcc ccgcccgacg cgaccttcgc
     1561 gagcggttgc gcgggctgcc ccggcccgcc cgagaacacg aaggccgcgt gctcggcccc
     1621 gccggagacg agctccgggt tggggaccaa ggtgacctcg atcgtggcgc cctccatccc
     1681 gagctcgccg agctcccctc ggagcgcccg cgtgagctcg ggcgccgcgg tggcgcgacc
     1741 gtccgagacc gccgtggcga gcgcggcgac gcgctcgttg agctcggcga ccctggccgc
     1801 ggcgcgctcc cgctcctgct cgacgccggc cagcgcttcg aggctctccc gtgcccggtc
     1861 gaggaaggcg agcacctcat cctcgccctt cccgtacttg cgctggagcg cgtcgagtgc
     1921 gtcgatccgc tcgcggacct cgtgcagccg ctcggggtcg agctcgagcc gttcgcggta
     1981 ggtgcgaacc tccaccgcga gctcggtggc cgcggcgctc aggtcgaacg cccgcaccgc
     2041 gagctcctcg gcccccgggt cgacctcgcc cgccgtccgg agctcggcgg ccgcgacccc
     2101 gagggcatcg gccgcgccgt cctcggccgc gagggcacgc tcggccgacg aggcccgctc
     2161 gagcaggcgg ccggcgtggt cgagcctggc tgcctcgagc ttcagctcct cggtctcgcc
     2221 cggccgcggc gacgcgccct cgatctcgcg cacctggtag gcgagcaggt ccatctggcg
     2281 ctcgcggtcg cgctcgcgct cgtcgagctc ggcgagggcg gcccgcgcgt cgcggagacg
     2341 cccgtgctcg acgcgaaggg cgtcgacggc gtcgaggtgc tcggcgccgg cgtagcgatc
     2401 gaggaaccgc gtctgcgcaa ccgtggatag caaccgctga ccgtcgtgct ggccgtggat
     2461 ctcgacgagc tcgcgggcca gctccgcgag cgccgagacc ggcgcgagct gtccgcccac
     2521 acgcgcggtg ctccgtccct cggcggtgac cgtccgcgcg agcaggacct cgccgtcctc
     2581 ggcccacccc gcgctcacgg ccgccgacgg cgcgtcgaac ctcgcctgca ccttcgccgc
     2641 cggcgcgccc actcgaacga gcgacgaacc cccgcgccgg ccgagcgcga gctgcagccc
     2701 gaccgtcacc atcgtcttgc cggtgcccgt ctcgccggtg aggacgttca ggccctgatg
     2761 cagctcgagg tcgaggtcgt cgatcacgcc gagaccgctg atgtggagat cgcggagcac
     2821 cccgcgatcc tagaccgctc cgccgacgct cccggggagg tcgaacttgt cgcggacgag
     2881 gtcgtggaac gacggagcgt cctcgcgccg aacgaaccgc gcgggcgtcg gggcgcgttc
     2941 gacctggacc gtcgagccga ccgggagctc caggctgtgc cggccgtccg ccgagagcaa
     3001 ccccggttcc tcccccaaca cctcgaggcg cacgcgttgg tcgggcgcga gcaccagcga
     3061 ccggtcgaac accatgtggg ccgcgatcgg cgtcacgacg atgcagggga cggacggcga
     3121 gacgatggga cccccggcgc tgaacgagta cgcggtcgaa ccggtcggcg tcgccgagat
     3181 caccccatcg gcggagaagg tggtgacgta cgagtcgtcc acgaacaccg cgagccggat
     3241 cagtctgtgc cgagcgctct tctccacgat gacctcgttg agcgcccact gcggctcgaa
     3301 cgacgagccc tccgccacaa ccgcgagccg ctcctcgatc cgggccgagc cgtccagcat
     3361 ccgttcgagc accgccgtgg cctggccggg ctcgacctcg gtaaggaagc ccatccggcc
     3421 gaccttgacg cccagcaccg ggacgcccga caccgatgcg accctcgccg cccgaaggaa
     3481 cgtgccgtcg ccgcccaccg acaccacgag gtcgaggccg cgggcgaacg cccgcgccgc
     3541 ctcttcgtcc tcggcgtcgt cgccggcgat cgtgcgggtc tggaccgcgc ggtcctcgag
     3601 ccatccgccg atcgatcgcg cggcctcggc cgcggcccgg cgggccgggt ggacgacgaa
     3661 ccccgcggcc ctcaaccgcg agcctgaggc cgaggacgtc acgagggcac ctccgcgatc
     3721 gcgcgggcct cctcgagcgc gccctccacg tcgcggacga cggtcctcgc cgagccgacc
     3781 cttgcgtgca gcgggaactc gatgttgccg gccggcccgg gcagcggcga cgccatcacg
     3841 gcgatcggcg agaggccgtg ctcggagacg gcatcggcga cgccgaggat cgcgtcgcgc
     3901 caggcgtccg ggtcgcggac gacgccgccc gaccccaccc gcccgcggcc gacctcgaac
     3961 tgcggcttga ccaacaggac cagatcggcg tcgctcgacg agacccgggc gagcgccggg
     4021 atggcgaggc ggagcgacat gaaggacagg tcggcgacca cgatgccggc ggcgtaggga
     4081 aggtcctcgg gacgcagatc gcgcacgttg gtccgatcga gaaccgtgac gcgtcggtcg
     4141 gttcggagcg accaggcgag ctgcccgtat ccgacgtcga ccgccacgac gtgcgccgcg
     4201 ccccgacgga ggaggcagtc cgtgaagccg ccggtggacg cccccgcgtc cagaccgtcg
     4261 cgaccatgta cgtcgatcgc gaaacgttcg agagcggcct ccagcttcac gccggcgcgg
     4321 gacgcgaagc gtcgcggctc cccgacgagc tcgagtggct ccgcggcgtc caccagggta
     4381 gagggcttgc ttgccgaccg gcccgagacc aggaccaacc catcgcggac ggcgctctgc
     4441 gcctggctgc gggtggcgac gaggccccgg cgcacgagct cggcgtcgag tcgccggcgc
     4501 gccacggcgc gcagcgtcag gcctgctcgc ttggagg
//
LOCUS       DATBRK010000075         6346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_154331, whole genome shotgun sequence.
ACCESSION   DATBRK010000075 DATBRK010000000
VERSION     DATBRK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154331"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VIH70_02315"
     CDS             <1..270
                     /locus_tag="VIH70_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8115230.1"
                     /translation="HASRAEVRLLQSDAALVFEVDDDGAGFDPGSTGYGTGLQGIADR
                     LAALDGRIEIRSRSGGGTVVTGTIPLGSDPARDGVPTRTVERDEA"
     gene            270..2336
                     /locus_tag="VIH70_02320"
     CDS             270..2336
                     /locus_tag="VIH70_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8115231.1"
                     /translation="MGGLARSRPSRWPWVALGVFFSLAITGMIGVAVNGESLAGQIPY
                     VIAFTLFGIVGALLLSRLPGSRVGGLLLFGAAVTAGSFAAGELMTYLVREGNTDGPAV
                     VAMALVSSLGWLVGIFPVILLLPLLFPDGRLPSRRWLPFAWLCAAVLAFLGVMLLFGQ
                     DTLFGSSDDAVIANPFFMPGLAGLEIPDAVFGLSLIGLLVGSVVSLVLRFRRSSGVER
                     QQIKWVALAVAFLASSFVLSTVASAIGLNGDLIDSLVSGIAFITLPVAVAVAVLQYHL
                     YELDVVVKKALVAGALVLLVIAAYAAVVGLLGVIASGNESSGSVFVVALLLGLAFRPV
                     TRFARRIADRVVYGRRATPYEVLTEFSERVGESYATEDVLGRMAQILGQATGAGSARV
                     WLRLGAELRPAASWPTDAEPAAVAPVANEVLPTIEGETVVEVRDRGELLGALSVAMPP
                     SDPMNPSKERLVRDLASQAGLVLRNVRLVEELRASQRRLVTAQDQERRRLERNIHDGA
                     QQQLVALSVRTRLARDLTARDPARAAEMLAQIDAEIQRALDDLRDLARGIYPPLLIDE
                     GLGAALDAQARRSPVPTTIRADGLGRYAPEVEAAVYFSCLEALQNVAKYAEASSATVI
                     LVQSNGHLTFEVVDDGRGFDPTAERSGTGLQGIADRLGALHGEVTIRSEPGAGTRVRG
                     RIEVGA"
     gene            2333..3049
                     /locus_tag="VIH70_02325"
     CDS             2333..3049
                     /locus_tag="VIH70_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115232.1"
                     /translation="MRRAFRIVAAVLGIVTIGFNLPFAVGALLGEADRIHRFHFVAGT
                     LGSGVLLGVSLLACARRPEDLAPFWVAVASGVATTIGGIIAGDFISGLYVIAPILLIV
                     LFALHPFRRSLFAIGGVDVAAGALAALAVVPAVAYALTQAELQRNGSSLDPHVEFHHY
                     SGMTTYALSLPLAALAAALRLPGRRIAVWIVGVTGAGLGLASLLLSDYVGAFEPVWAW
                     LALAWGVGFVAVGQLRRGSA"
     gene            3046..3258
                     /locus_tag="VIH70_02330"
     CDS             3046..3258
                     /locus_tag="VIH70_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115233.1"
                     /translation="MTVERTRPKSIWRRIERWMVGLVMAVIAFVLEKMVMRGIKKGAK
                     AKGEGKDELHTTLTSKGGEVDLEDAR"
     gene            complement(3255..5363)
                     /locus_tag="VIH70_02335"
     CDS             complement(3255..5363)
                     /locus_tag="VIH70_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY8115234.1"
                     /translation="MAGGNASGGAPGVARVMVGTDRSETAERAVRWAAAFADAYSAEL
                     HVVQVVIPLHPADTEHGTAEVTRARAAADELQTYATTIAGGRGFAHVVIDDDPALAIV
                     HATEEHDIDVLVVGNAGMAGRKEFLLGNVPNRISHNARCTVIIVNTTRDGEVATATRP
                     STTIRSSQVETPTEPHLMSRGTKIATVFAKHGLKELFGRPDADGAVGRRRQAKRLRSS
                     LEELGPTFAKLGQILSTRPDLVPPEFIEELATLQDNVPPLTEEQVVRVMEQELGVPWE
                     DVFETIDPQPLAAGTIAQVHRATLADHTRAVVKVQRPDAKEQIEQDLALLEVFAEKVA
                     ERPGLRQVVDMQAVFEHLSESLHRELDFRREAENIDRMGSVIEGFTRLRVPVVHADYS
                     TSRLLVMQDIGGGPATSAPEGPARREAARQLLESFYKQILVDGFFHADPHPGNLMWQP
                     EEETLYFLDLGMIGEVGPDLREHMMLLLMAFWQQDVGFLTDVTLMLTGAINRTDLDVE
                     AFQEELGALMARNRGVSIKEIQLGPVLQEMTEIALRHDVALPASLTLTAKALAQMQLA
                     AATLDPDVDPFDVAGKFLMRNLVRSMGAKFDPKTLFYQAQRFKVRFEKVVEAIERLIG
                     ARPGQKLEVNFRAATLETTIRRAGRRLAISLTAGAAILASALTAVSSDRVARWVPVAF
                     GVISAGLLLALLVDLIRKRG"
     gene            5467..5543
                     /locus_tag="VIH70_02340"
     tRNA            5467..5543
                     /locus_tag="VIH70_02340"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5501..5503,aa:Thr,seq:cgt)
     gene            5597..6274
                     /locus_tag="VIH70_02345"
     CDS             5597..6274
                     /locus_tag="VIH70_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115235.1"
                     /translation="MLGQVSVDPTVHPVLNNGGWVFNAAELLALLGVVLTVAIVFGYM
                     RLAPRFQTDEQTRRPVRVPRMQPGGEVRRPVKVIGTPIIVQPPVIAPAVAGGPAANAA
                     RAVAAPASAAPLAAPAPSAAPAAQPAPAAEAAPAAEAAPAAEAAPAPDAAAPAEAAPA
                     AEAAPAAEAAPPAAQPAAAHPDVSLDQETFDRVLAELLEKGTDRRVAEGQARRAAMIA
                     ARKKAEG"
BASE COUNT          826 a         2231 c         2275 g         1014 t
ORIGIN      
        1 cacgcatcac gcgcggaggt tcggctgctc cagtcggacg cggcgctggt gttcgaggtc
       61 gacgacgacg gggccgggtt cgatccgggt tccacggggt acgggacggg gctccagggc
      121 atcgccgacc gtctcgccgc cctggacgga cgcatcgaga ttcgctcgcg ctccggcggc
      181 ggaacggtgg tgaccggcac gatcccgctc gggagcgacc cggcgcgaga cggcgtcccg
      241 acacgtaccg tggaacggga cgaggcgtga tgggggggct ggcgcgctcg aggccctccc
      301 ggtggccgtg ggtcgcgctc ggcgtgttct tctcgctcgc gatcacgggc atgatcggcg
      361 tcgcggtcaa cggggaatcg ctcgccggac agatccccta cgtgatcgcc ttcacgctct
      421 tcggcatcgt cggcgcgctc ctgctcagcc ggctgccggg cagtcgggtc ggtggtctcc
      481 tgctgttcgg ggcggcggtc accgcgggaa gcttcgccgc cggggagctc atgacctacc
      541 tcgtgcgcga gggcaacacg gatggcccgg ccgtggtggc gatggcgctc gtgtcgtcgc
      601 tcgggtggct ggtcggcatc ttcccggtga tcctcttgct gccgctgctg ttcccggacg
      661 gacgcctgcc gtcccgccgt tggctgcctt tcgcctggct gtgcgcggcg gtgctcgcgt
      721 tccttggcgt gatgttgctc ttcggtcagg acaccctgtt cggctcgtcg gacgacgccg
      781 tcatcgcgaa ccccttcttc atgccgggcc tcgccggcct cgagatccct gacgcggtgt
      841 tcggcttgtc gctcatcgga ctgctcgtgg gcagcgtcgt ctccctggtg ctccggttcc
      901 gtcggtcgag tggcgtcgag cgacagcaga tcaagtgggt cgcgctcgcg gtcgccttcc
      961 tggcgagctc cttcgtcctg tcgacggtcg cctcggcgat agggttgaac ggcgacctga
     1021 tcgactcgct cgtgagcggg atcgcgttca tcaccttgcc cgtcgccgtg gcggtcgccg
     1081 tcctgcagta ccacctgtac gagctcgacg tcgtggtgaa gaaggcgctc gtcgccggcg
     1141 cgctcgtcct gctcgtgatc gccgcctacg cggccgtcgt cggactcctg ggcgtgatcg
     1201 cctccggcaa cgagagctcc ggctccgtgt tcgtcgtcgc gctgctgctc ggtctcgcat
     1261 tccggccggt gacgcggttc gcgcggcgga tcgccgatcg cgtcgtctac ggcaggcggg
     1321 cgacccccta cgaggtgctg accgagttct ccgaacgcgt cggcgagtcg tacgcgacgg
     1381 aggacgtcct cggtcgcatg gcgcagatcc tcggccaggc caccggcgcc ggctcggcca
     1441 gggtgtggct ccgcctgggc gcagagctac gcccggcggc ctcgtggccg acggacgccg
     1501 agcccgcggc ggtcgccccg gtcgcgaacg aggtcctccc gacgatcgag ggcgagaccg
     1561 tcgtcgaggt ccgtgaccgg ggcgagctcc tcggggcgct ctcggtcgcg atgccgccca
     1621 gcgatccgat gaacccgtcg aaggaacgcc tcgtccgcga tctcgcgtcg caggccggcc
     1681 tggtccttcg caatgtccga ttggtcgagg agctgcgcgc atcacagcgg cggctcgtca
     1741 cggcgcagga ccaggagcgc cgcaggctcg aacgcaacat ccacgacggg gcgcagcagc
     1801 agctggtcgc gctgtccgtg aggacccgcc tggcgcgcga tctgacggcg cgcgatccgg
     1861 cccgggcggc cgagatgctc gcgcagatcg acgccgagat ccagagggcc ctggacgacc
     1921 tccgcgacct ggcgcgcggg atctacccgc cgctcctgat cgacgaggga ctcggcgcgg
     1981 cgttggacgc ccaggcgcgg cgatcgcccg tgccgaccac gatccgggcc gacggcctgg
     2041 ggcgctacgc gcccgaggta gaggcggccg tctacttctc gtgcctcgag gccctgcaga
     2101 acgtggccaa gtacgcggag gcgagctcgg ccacggtgat cctcgtgcag tcgaacgggc
     2161 acctgacgtt cgaggtcgtg gacgacggcc gcggattcga ccccacggcc gaacggagcg
     2221 gtaccggcct gcaggggatc gccgaccggc tcggggcgct ccacggcgag gtcacgatcc
     2281 gttccgaacc cggtgctggc acgcgtgtcc gagggcggat cgaggtgggg gcatgaggcg
     2341 agcgttccgg atcgtcgcgg ccgtcctggg catcgtgacg atcggcttca acctgccgtt
     2401 cgcggtgggc gcgttgctgg gcgaggccga tcggatccac cggttccact tcgtcgcggg
     2461 gacgctgggg tcgggcgttc tgctcggtgt gtcgctcctc gcctgtgcgc gcaggcccga
     2521 ggaccttgcg ccgttctggg tcgccgtggc ctcgggcgtc gccaccacga tcggggggat
     2581 catcgccggc gacttcatct ccggcctgta cgtcatcgcg cccatcctgc tcatcgtgct
     2641 gttcgccctg catccattcc gccgttcgct gttcgcgatc ggcggcgtgg acgtcgccgc
     2701 cggtgccctg gccgcgctcg cggtggtgcc ggcggtcgcg tacgcgctca cgcaggccga
     2761 gctccaacgc aacggctcct cgctcgaccc ccacgtcgaa ttccatcact acagcgggat
     2821 gacgacctac gcgttgtcgc tcccgctggc cgcgctcgcc gcggcgctgc gactgcccgg
     2881 tcgacggatc gcggtgtgga tcgtgggcgt gacgggcgcc gggctcggcc tggcctcgct
     2941 cctgctctcg gactacgtgg gcgcgttcga acccgtgtgg gcgtggctcg cgttggcgtg
     3001 gggggtcggg ttcgtggccg tggggcagct ccgacggggg tcggcgtgac cgtcgaacga
     3061 accaggccga agagcatctg gcgcaggatc gagcggtgga tggtcggcct cgtgatggcg
     3121 gtgatcgcgt tcgtgctgga gaagatggtg atgcgcggca tcaagaaggg cgcgaaggcc
     3181 aagggcgagg gcaaggacga gctgcacacc accctcacct ccaagggcgg agaggtcgac
     3241 ctcgaggacg cccgctagcc gcgtttgcgg atcaggtcca ccagcagcgc gaggagcaga
     3301 ccggcggaga tcacgccgaa ggccaccggc acccacctgg ccacccggtc cgaagagacc
     3361 gcggtgagcg cggaggccag gatcgccgcg ccggccgtga gcgagatcgc caggcggcgg
     3421 cccgctcggc ggatcgtggt ctcgagcgtg gctgcacgga agttcacctc gagcttctgt
     3481 ccggggcggg ctccgatcag gcgttcgatc gcctcgacca ccttctcgaa gcggaccttg
     3541 aaccgctggg cctggtagaa gagcgtcttc gggtcgaact tggctcccat gctgcgcacg
     3601 aggttgcgca tcaggaactt gcccgcgacg tcgaacgggt cgacgtccgg atcgagggtc
     3661 gccgcggcga gctgcatctg cgcgagcgcc ttggccgtca gcgtgagcga ggccgggagg
     3721 gccacgtcgt gccggagcgc gatctcggtc atctcctgca gaaccggccc gagctggatc
     3781 tccttgatcg agacgccgcg gttgcgcgcc atcagcgcgc cgagctcctc ctggaacgcc
     3841 tcgacgtcca ggtcggttcg gttgatcgcg ccggtcagca tcagcgtgac gtcggtcagg
     3901 aacccgacgt cctgctgcca gaacgccatc aggagcagca tcatgtgttc gcgcaggtcg
     3961 ggtccgacct cgccgatcat gccgaggtcg aggaagtaca gcgtctcctc ctcgggctgc
     4021 cacatcaggt tccctgggtg cgggtcggcg tggaagaagc cgtcgacgag gatctgcttg
     4081 tagaaggact cgagcagctg ccgggcggcc tccctgcgcg ccggcccctc gggcgcggac
     4141 gtggccggcc cgccgccgat gtcctgcatc accaggagcc gggaggtcga gtagtcggcg
     4201 tgcaccaccg gcacccggag gcgcgtgaag ccctcgatca ccgagcccat ccgatcgatg
     4261 ttctcggcct cgcgccggaa gtcgagctcc cggtgcagcg actcggacag gtgctcgaac
     4321 acggcctgca tgtccacgac ctgcctgagg ccgggacgct cggccacttt ctcggcgaag
     4381 acctcgagca gcgcgaggtc ctgttcgatc tgctccttcg cgtcggggcg ctgcaccttg
     4441 acgacggccc gggtgtggtc ggcgagcgtc gcgcggtgca cctgcgcgat cgtgccggca
     4501 gccaggggtt gcgggtcgat cgtctcgaag acgtcctccc agggcacgcc gagctcctgc
     4561 tccatcacgc ggacgacctg ctcctcggtc aggggcggga cgttgtcctg cagggtcgcg
     4621 agctcctcga tgaactcggg cggcaccagg tccggccgcg tcgacaggat ctggccgagc
     4681 ttggcgaagg tcggcccgag ctcctcgagc gagctccgca gccgcttggc ctgccgccgg
     4741 cgacccacgg caccgtcggc gtcgggccgg ccgaacagct ccttcagccc atgcttggcg
     4801 aagaccgtgg cgatcttcgt cccgcgcgac atcaggtggg gctcggtcgg ggtctcgacc
     4861 tggctcgatc ggatcgtggt ggacggccgc gtcgccgtcg cgacctcccc gtcgcgcgtc
     4921 gtgttcacga tgatcaccgt gcagcgcgcg ttgtggctga tccggttcgg gacgttgccg
     4981 agcaggaact ccttgcggcc cgccatcccg gcgttgccga cgaccaggac gtcgatgtcg
     5041 tgttcctcgg tcgcgtgcac gatcgcgaga gccggatcgt cgtcgatcac cacgtgggcg
     5101 aagccgcgcc cgcccgcgat cgtggtcgcg taggtctgga gctcgtcggc ggcggcacgg
     5161 gcccgcgtga cctcggccgt gccgtgctcg gtgtccgcgg gatgcagcgg gatcaccacc
     5221 tgcacgacat gcagctccgc cgagtacgcg tccgcgaacg cggccgccca ccgcacggcg
     5281 cgctcggcgg tctcggagcg gtccgtgccg accatcacgc gagcgacgcc cggtgctcca
     5341 ccgctcgcgt tacctcctgc catgacgccg catcatccca gacgggactc ggagcgagga
     5401 aacccatggc cgccagggcg ttcgggtgcc agcagccgcg ggtgcttcgt agactccctg
     5461 ccgtccgccg gagtagctca gtcaggcaga gcaaccgcct cgtaagtggt aggtcagggg
     5521 ttcgaatccc ctctccggct ccagtcgcgt ccaacgcaaa gcgggatcac gagctcctct
     5581 tgctagaatc gccgccgtgc tcggccaggt gagcgtcgac cccaccgtcc accccgtcct
     5641 gaacaacggt ggctgggtct tcaacgccgc cgagctgctc gcgcttctcg gtgtcgtgct
     5701 cacggtcgcg atcgtcttcg ggtacatgcg gctcgcccct cggttccaga cggacgagca
     5761 gacgcgccgg ccggttcggg tgccgaggat gcagccggga ggggaggtac gccggcccgt
     5821 caaggtgatc ggaacgccga tcatcgtgca accgcccgtg atcgcgccgg ccgtcgccgg
     5881 aggccctgcg gccaatgccg cacgcgccgt cgccgctccc gcttcggccg cgccgctggc
     5941 cgccccggca ccctcggcgg ctccggccgc tcaacctgcg ccggccgcgg aagccgcccc
     6001 agccgcggaa gcagcgccgg ccgcggaagc cgccccagcc cccgatgcag cggcgcccgc
     6061 ggaagctgcg ccggccgcgg aagccgcccc tgctgccgag gcagcgccgc ccgcggccca
     6121 gcctgccgcc gcccacccgg acgtgagcct ggaccaggag acgttcgatc gcgtcctggc
     6181 cgagctgctc gagaagggga ccgatcgacg cgtcgccgag ggccaagctc ggcgcgcggc
     6241 gatgatcgca gcccgcaaga aggccgaggg ctaggccctc tccggccatg gaggccacca
     6301 tcgactggtc catgggttct cgcgccgccg cgctcatcga ctgggg
//
LOCUS       DATBRK010000076         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2609741, whole genome shotgun sequence.
ACCESSION   DATBRK010000076 DATBRK010000000
VERSION     DATBRK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609741"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VIH70_02350"
     CDS             complement(<1..1221)
                     /locus_tag="VIH70_02350"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8115236.1"
                     /translation="MRSNPARPRLLLVALALAALLAACTEASGEPSSRPTGTAALSGS
                     PSPGADLTKLEQAKANLKHLVFIVQENRSFDHYFGTFPGADGIPMRDGKPTVCVPDPI
                     TNRCVRPYHTSAQLQEGGPHGQRHSVLDVDGGRMDGFVRTAIDSPLYCADHRSAPRCA
                     GYLGPQGQPDVMSYHTAKEIPNYWRYAKEFVLQDHMFAPADSWTLPAHLFLVSGWAAH
                     CANPRDPMSCVSNLDLVEEFQTMRSRADVPIWAWTDITYLLHEHGVPWGYYVGDETCF
                     FDPCPPDPRENTVPQQDPLPWFTTIRETGQMDRIQTHSQFFQQAANGTLPSVSWVMPY
                     NGVGEHPASGAPIWKGQRHVTNVVNALMRGPDWNETAIFLTWDDWGGFYDHVEPPRVD
                     LNGYGIRVPGLLISP"
     gene            1330..>2075
                     /locus_tag="VIH70_02355"
     CDS             1330..>2075
                     /locus_tag="VIH70_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY8115237.1"
                     /translation="MTATPTDPTVVTLIPGDGIGPECVAAARRIIEAAGAPITWEDCE
                     AGAEVFRKGLPSGVPPETIESISRTRVALKGPLETPVGFGEKSANVTLRKLFETYANV
                     RPVREMPGVRTPYSDRGIDLVMVRENVEDLYAGIEYLQTPGVAQALKLISEKGSEKIA
                     RFAFEYARSEGRTKVACATKANIMKLSEGTLKRVFERVAPEYPELESWHVIVDNCAHQ
                     LVKRPEQFDVIVTTNMNGDILSDLSSALIGG"
BASE COUNT          340 a          682 c          732 g          321 t
ORIGIN      
        1 cgggctgatc agcaggccgg gcacgcggat cccgtagccg ttcaggtcga cgcgcggggg
       61 ttcgacgtgg tcgtagaacc cgccccagtc gtcccacgtg aggaagatcg ccgtctcgtt
      121 ccagtccggg ccgcgcatca gtgcgttcac gacgttggtg acgtgtcgct ggcctttcca
      181 gatcggcgcg cccgaggccg gatgctcgcc cactccgttg tacggcatga cccacgaaac
      241 cgagggaagc gtcccgttcg cggcctgctg gaagaactgg ctgtgcgtct ggatccgatc
      301 catctgcccc gtctcgcgga tcgtcgtgaa ccacggcagt gggtcctgct gcgggaccgt
      361 gttctcccgg ggatcgggcg ggcacgggtc gaagaagcac gtttcgtcgc cgacgtagta
      421 cccccagggc acgccgtgct cgtgaaggag gtaagtgatg tcggtccacg cccagatcgg
      481 aacatccgct cgggatcgca tcgtctggaa ctcctcgacg aggtcgaggt tcgagacgca
      541 cgacatcggg tcgcgtgggt tcgcgcagtg ggcggcccat cccgacacca ggaacagatg
      601 cgccggcagc gtccacgagt ccgcgggcgc gaacatgtgg tcctgcagga cgaactcctt
      661 cgcgtatcgc cagtagttcg ggatctcctt cgctgtgtgg tagctcatga cgtcaggttg
      721 cccctgcggc ccgaggtacc ccgcgcacct cggagccgag cggtgatcgg cgcagtagag
      781 cgggctgtcg atcgcggtcc tgacgaaccc gtccatcctc ccgccgtcga cgtcgagcac
      841 cgagtgacgc tggccgtgcg gccccccctc ctggagctgt gcgctcgtgt ggtagggacg
      901 gacgcatctg ttggtgatcg gatcggggac gcagaccgtc ggcttcccgt ctcgcatcgg
      961 gatgccgtcg gcccccggga acgtcccgaa gtaatgatcg aacgaacggt tctcctggac
     1021 gatgaagacc aggtgcttga ggttcgcctt ggcctgctcg agcttcgtca ggtcggcacc
     1081 gggcgagggc gacccggaga gcgcggccgt gccggtcggc ctcgagctcg gctcgcccga
     1141 ggcctccgtg caggcggcga gtaacgcggc cagggcgagc gcgacaagga gcagacgcgg
     1201 acgggcgggg ttcgaacgca tgaaccgccg agggtaccaa tcgtcgcccg atcgggcgag
     1261 gctcaacctc gcggtcgtgc gggggctcgg cgctaggatg accgcccatc ccggcccgaa
     1321 ggagctcacg tgaccgcgac gccgaccgac ccgaccgtcg tcacgctcat ccccggagac
     1381 gggatcgggc ccgagtgcgt ggccgccgcg cgccgcatca tcgaggcggc cggcgcgccg
     1441 atcacctggg aggactgcga ggccggcgcc gaggtcttca ggaagggtct gccgtcgggc
     1501 gtcccgcccg agacgatcga gtcgatctcg cggacgcgtg tcgccctcaa ggggccgctc
     1561 gagacacccg tgggcttcgg ggagaagtcg gccaacgtca cgctccgcaa gctgttcgag
     1621 acctacgcca acgtccggcc ggtccgcgag atgccgggcg tcaggacccc gtacagcgac
     1681 cggggcatcg acctggtgat ggtgcgggag aacgtcgagg acctgtacgc ggggatcgag
     1741 tacctgcaga caccgggcgt cgcccaagcg ctcaagctga tcagcgagaa gggctcggag
     1801 aagatcgcgc ggttcgcgtt cgagtacgcc cggagcgagg gacgcaccaa ggtggcctgc
     1861 gcgaccaagg ccaacatcat gaagctttcc gagggcacgc tgaagcgcgt gttcgaacgc
     1921 gtcgcgccgg agtacccgga gctcgagtcc tggcacgtga tcgtcgacaa ctgcgcgcac
     1981 cagctggtca agcggccgga gcagttcgac gtgatcgtca ccaccaacat gaacggcgac
     2041 atcctgtcgg acctgtcctc ggcgctgatc ggcgg
//
LOCUS       DATBRK010000077         8543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1885604, whole genome shotgun sequence.
ACCESSION   DATBRK010000077 DATBRK010000000
VERSION     DATBRK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885604"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VIH70_02360"
     CDS             <1..375
                     /locus_tag="VIH70_02360"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8115238.1"
                     /translation="VFDVVIHAGDFASWNPTIKSSRQLSPDPIGEGTTFEWDLRGFGA
                     VRQELREFEPNHRVRIVPHHRSLAGGHRFTFTDLGGRTRVDHELEMIPKGAYKLLAPM
                     MSMVGKRNLRATANALDERLRS"
     gene            complement(425..1633)
                     /locus_tag="VIH70_02365"
     CDS             complement(425..1633)
                     /locus_tag="VIH70_02365"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEY8115239.1"
                     /translation="MDEPSVFRRLWQPPGRYADRDVDRRVTFLELFFDLVFVVVISQL
                     ARRLAAHPSWAGVGWFVFLFYAVWSSWMNGTLYHDLHTTNDVSVRVFTFGQMLAVAVM
                     AVFIGDVPGEGSTGFALAYAANTLILVVLWFRTGLHDPDHRPGSFPYSAGYLAAAALF
                     VVSVAFDGPARYWLWAVALAFEVAGWVIGMYRWKPPASQGGDARIATTPSLIERMGLF
                     VIIVLGEVIVGAVTGMADVHPLEAEGIVIGLLGVVVAIGLWWIYFDLVSYRAPVPRRT
                     QLWLNLHFPMVVAIAAGGAAVLNTVEHAGRPLPDAVRWLLVGSLAAAMLSDALIAWTL
                     EIRRTLPELYRTAEIALPVGAFLCLAVGLTAWGAKGSLSAMVVLLLAPVAAGTAVWLK
                     HPERDTVDLG"
     gene            complement(1651..1992)
                     /locus_tag="VIH70_02370"
     CDS             complement(1651..1992)
                     /locus_tag="VIH70_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8115240.1"
                     /translation="MSDDERGGAPISIPVAAKGRVRPFEPTDLVVVNDAVVRIAAIEG
                     EFPWHHHDEDELFLCWDGGFRIEFRDGEPVELVTGDLYVIPRGIEHRPVAERRAVVLL
                     LERPETRQYGN"
     gene            2173..2748
                     /locus_tag="VIH70_02375"
     CDS             2173..2748
                     /locus_tag="VIH70_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010491763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8115241.1"
                     /translation="MDEGAVRVEEGTIRIEEPAKRVEHFSKIGPCLWFDDQAEPAADF
                     YVKVFSARRATAGDGGESKILGISRYGDAGPGPAGTVMTVDFLLEGQEFVALNGGPEF
                     SFTPAISFIVNCETQDEVDGLWETLSQGGTQGECGWLEDKFGVSWQIVPTALGELLAD
                     PDPERTQRVMRAMLKMRKLDIAGLERAAEAT"
     gene            2836..3330
                     /locus_tag="VIH70_02380"
     CDS             2836..3330
                     /locus_tag="VIH70_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115242.1"
                     /translation="MRRNLLVAMLALGLVVVGARAVIAGTATGRTPVDCIDTIWRSST
                     ISTSSTAWETVPGFEADPIAIFPITVNVSALVSGAPARFRILSTNVGEQTVVSNPGAT
                     RFEPGAAGPNSFSFQWVERDDVAATHANLIRLQWRSPSGDQVSMRRGDMAVLYRTDGC
                     DGSS"
     gene            complement(3353..4303)
                     /locus_tag="VIH70_02385"
     CDS             complement(3353..4303)
                     /locus_tag="VIH70_02385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY8115243.1"
                     /translation="MKAFGLADEGASAAVIEVPIPEPGPGEVRVKVRASSVNGFDVFV
                     AKGMAKGMMEHRYPVVIGKDYAGEIDALGEDATGFAVGDEVTGIVPTEPHLWRGAYAE
                     FVAVPLDGFVVHKPGNLDFERSACIGLAAITASNCVETIEAAEGDVMLIVGATGGVGT
                     YSVQLAADRGARVVASALHRDEAWIRGLGASEIVDYSGDVVAAMLERYPEGVDGLIVA
                     AHVGDGFGALTELVKSGGRIASTVGGADVDALAQRGVAAANVTGHADPQAFGRLVQMA
                     GDGTLSVPITRTFTFDELPRALRLVGEESSRGKFAIAFAS"
     gene            4480..5478
                     /locus_tag="VIH70_02390"
     CDS             4480..5478
                     /locus_tag="VIH70_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016579070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8115244.1"
                     /translation="MEELTARDRPAHPGSDELELRLEEHRTELTAYAYRMLGSGFEAE
                     DAVQEAFLRAWKSFEQFEGRSSLRSWLYSIVTNVCLDMLGGRERRARPMELAPARSAD
                     IPLPDPLPENVWVLPVPDDRVVPESGDPSEVLEARESVRLAFVAALQHLPPRQRAVLI
                     LREVLRWKASEVAELLDTSVASVNSALQRARATIESLEIEDAETLRPADDEQRALLDR
                     YVDAFERYDMDSLTSLLREDATWSMPPYEMWLHTHDDIRAWCLGPGIGCRGSRLIAVE
                     ANGMPAFGQYKPAEGGLEPWSLQVLEVSDGRIDGISFFLDTERLFPQFGLPPRLDG"
     gene            complement(5368..5856)
                     /locus_tag="VIH70_02395"
     CDS             complement(5368..5856)
                     /locus_tag="VIH70_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8115245.1"
                     /translation="MTANERASPPEARAWSANQPVARRWRPGPGGTVLVMSGPVTRDA
                     IPALCERAERLLEGCDAGPVACDVGALAEPDATTIDALARLQLTARRLGYRVELRGTC
                     GELEDLLRLAGLLEVLTGGGAVVASAVEAWGKPELREQAFRVQEEADPVDPTVGDLQD
                     LQ"
     gene            complement(5853..6719)
                     /gene="larE"
                     /locus_tag="VIH70_02400"
     CDS             complement(5853..6719)
                     /gene="larE"
                     /locus_tag="VIH70_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEY8115246.1"
                     /translation="MSAVAERARVAVIRDLEDRIRGYGDRALVAFSGGVDSSVVLALA
                     ARALGPARVEAVTAISPSYAAGELEVAARTASDLGVAHRSVRTHEVEREAYARNDELR
                     CYHCKAELYGVLGALVRTAIPDTAVLAGANADDLDEFRPGIWAGERAGIRNPLLEGSV
                     GKATVRSIARTLGLRVSDKPALACLSSRVELGIRISPELLARIDEAEAIVRALGFEVV
                     RVRHRGASSTVEVPAGEVDRLLAHPERDAALRAVAALGWDEVAVDPNGYRPGGATPRA
                     DEFRAPDRSHRR"
     gene            complement(6716..7906)
                     /gene="larC"
                     /locus_tag="VIH70_02405"
     CDS             complement(6716..7906)
                     /gene="larC"
                     /locus_tag="VIH70_02405"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEY8115247.1"
                     /translation="MNLLYVDVVGGAAGDMLLAALLDAGASRHEVSDAVSAVLGRRVE
                     CVTTEVRRRGLRALALSAPGDPSTERRRPRELLDAVERAALQDGLRAMATAVLRRLFD
                     AEAKVHGRGLDDLELEELGEDDTLLDVVGVAAALASLGVERVDVSAIPMPPPSNGGTH
                     GSPAPVMLELLSGFALRPSVAGMDLPETVTPTAAAILSELGRPVPEMPELVLERVGVG
                     AGTRDPASVANVVRVLLGSTSPPAARDRRLLVLEANVDDLGPELVPDAIDALLAAGAL
                     DAWTAPIVMKWGRPAVAISALCEREELDEVRRAFFEATTTLGVRVHEVSRPELDRRIV
                     EIDLAEGGPRIRVKLGVLEGRTLNAKPEHADVVEAARKLGRPVRSVHAQATALAYRLL
                     EEDR"
     gene            complement(7903..>8543)
                     /gene="larB"
                     /locus_tag="VIH70_02410"
     CDS             complement(7903..>8543)
                     /gene="larB"
                     /locus_tag="VIH70_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEY8115248.1"
                     /translation="ELRQGACEIVYAEGKTPEEVRSIVERLLVGNDGSIIVSRTSPEH
                     VDAVRAAAAGTAIEIVERPRARCVALRRNAPPRTGSVLIVTAGTSDLGVAEEAELTAS
                     MLGAETEIVADVGVAGLHRLAAAQPMLATADAVVVVAGMEGALASVVGGIAACPVIAC
                     PTSVGYGASLGGLAALLSMLSSCVPGVACVNVDDGVGAGTIAAMIARQSTPR"
BASE COUNT         1321 a         3109 c         2973 g         1140 t
ORIGIN      
        1 gtgttcgatg tcgtgatcca cgccggcgac ttcgcctcgt ggaaccccac catcaagagc
       61 tcgcgtcagc tctcgcccga tccgatcggg gaggggacga cgttcgagtg ggacctgcgg
      121 gggttcggcg ccgtccggca ggagctgcgg gagttcgagc cgaaccaccg ggtgcggatc
      181 gtccctcacc atcgatcgct cgccggcggc caccgcttca cgttcaccga tctcgggggt
      241 cgaacccgcg tcgaccacga gctcgagatg atccccaagg gcgcctacaa gctcctcgcg
      301 ccgatgatgt cgatggtggg caagcggaac ctccgcgcga ccgcgaacgc gctcgacgaa
      361 cgcctcagat cctgatcacc ggacgcaccc gaaccgttgg tcgatcctcc cgaaggccta
      421 cggctcagcc caggtcgacc gtgtcccgtt cggggtgctt gagccagacg gccgtgcccg
      481 ccgccaccgg tgcgagcagc agcaccacca tggcggagag gctccccttc gccccccagg
      541 cggtgagccc gaccgccaag cagaggaacg cgccgacggg cagggcgatc tcggccgttc
      601 ggtagagctc gggcagggtg cgtcggatct cgagggtcca ggcgatcagg gcgtccgaga
      661 gcatcgccgc cgcgagcgaa cccaccagga gccagcgcac cgcgtccggc aggggccggc
      721 ccgcatgctc gacggtgttg aggaccgccg cgccgcccgc ggcgatcgcg accaccatcg
      781 gaaagtgcag gttgagccag agctgggtcc ttcgtgggac cggagcgcgg tacgagacga
      841 ggtcgaagta gatccaccac agaccgatgg cgaccaccac ccccagtaac ccgatcacga
      901 tgccctcggc ctcgagcgga tggacgtcgg ccatgccggt caccgccccc acgatgacct
      961 cgccgaggac gatgatgacg aacagcccca tccgttcgat caacgacggc gtcgtcgcga
     1021 tgcgcgcatc gcctccctgg ctggcgggcg gtttccaccg gtacatgccg atcacccatc
     1081 ccgccacctc gaaggccagc gccaccgccc atagccaata ccgcgccggt ccgtcgaacg
     1141 cgacgctgac cacgaacaac gcggccgcgg cgagataacc cgccgagtac gggaacgacc
     1201 cgggccggtg atccgggtcg tgcagccccg tccggaacca caggaccacc aggatgaggg
     1261 tgttcgccgc gtacgcgagc gcgaaccccg tcgacccctc gcccggcacg tcgccgatga
     1321 acaccgccat caccgcgacg gcgagcatct gcccgaacgt gaagacgcgg acgctcacgt
     1381 cgttggtggt gtggaggtcg tggtacaggg tgccgttcat ccacgaggac cagaccgcgt
     1441 agaacaggaa cacgaaccag ccgacgccgg cccaggacgg gtgcgcggcg aggcgtctcg
     1501 cgagctgcga gatgacgacg acaaagacca ggtcgaagaa gagctccagg aacgtcaccc
     1561 ggcggtcgac gtcccggtcc gcgtaccgac ccggcggctg ccacaaccgc cggaacacgg
     1621 agggctcgtc catgggaacg aatctagccc tcagttcccg tactggcggg tctcggggcg
     1681 ttcgagcagc aggacgacgg cccgccgctc cgcgaccggc cgatgctcga tgcctcgggg
     1741 gatgacgtag aggtcccccg tgacgagctc gacgggttca ccgtcccgga actcgatccg
     1801 gaatccgccg tcccagcaca ggaagagctc gtcctcgtcg tggtgatgcc acgggaactc
     1861 tccctcgatc gccgcgatgc ggaccacggc gtcgttcacg acgacgaggt cggtcggctc
     1921 gaagggacga acccgcccct tcgccgcgac ggggatcgag atgggcgccc ccccgcgctc
     1981 gtcgtcgctc acgtcacgct cccgagcacg aggacaggtc tagcagacgc cgtcgacggc
     2041 gtcctccgga actcggcgag cggctccccc cgttcttgac gaaccgatcc aggctcccgt
     2101 ggttcgggtt cgggatcgaa agggaaaagc acgcaggagg aatgggcata ggagaaggag
     2161 ggagccatgg ggatggatga gggagccgtc agggtcgagg agggaacgat caggatcgag
     2221 gagccagcca aaagggtgga gcacttctcc aagatcgggc cgtgcctgtg gttcgacgac
     2281 caggccgagc cggccgcgga cttctacgtc aaggtgttct ccgcccggcg ggccacggcc
     2341 ggcgacggcg gcgagtccaa gatcctgggg atctcccggt acggcgacgc cggacccggg
     2401 ccggcgggca ccgtgatgac cgtggacttc ttgctcgagg ggcaggagtt cgtcgcgctg
     2461 aacggcggtc ccgagttcag cttcacacca gcgatctcgt tcatcgtgaa ctgcgagacg
     2521 caagacgagg tcgacggcct gtgggagacc ctgtcccaag gcggcaccca gggtgagtgc
     2581 ggctggctgg aggacaagtt cggcgtttcg tggcaaatcg tccccacggc cctcggagag
     2641 ctgctcgccg accccgatcc tgaacgcaca cagcgcgtga tgcgtgcgat gctcaagatg
     2701 cgcaagctcg acatcgccgg cttggagcgt gccgccgagg ccacgtaggg cgccaagagg
     2761 ccaccttttc cggggaggtc ccgtagcctc gaccgtccga ggcgcgggcg ggtccgcgcc
     2821 tggaaggagg cctcgatgcg caggaatctg ctcgtggcga tgctggcgct cggcctcgtg
     2881 gtggtggggg cgcgtgccgt gatcgcgggg acggcgacgg gacggacgcc ggtcgactgc
     2941 atcgatacga tctggcggtc ctcgaccatc tccacgtcgt cgacagcgtg ggagacggtg
     3001 ccggggttcg aggccgaccc gatcgccatc ttcccgatca ccgtcaacgt cagcgcgctg
     3061 gtctcgggag cgcctgcgcg gttccgcatc ctcagcacga acgtgggcga gcaaacggtc
     3121 gtcagcaacc ccggtgccac gcgtttcgaa cccggcgcgg ccggtccgaa ctcgttctcc
     3181 tttcagtggg tcgagcgcga cgacgttgcg gccacgcacg ccaacctcat ccgcctgcaa
     3241 tggcggagcc catcgggtga tcaggtttcg atgcgccgcg gcgacatggc ggtgctgtac
     3301 cgaacggacg ggtgcgacgg gtcgtcctag gcgagggtga tcgcgggccg cgtcagctcg
     3361 cgaacgcgat cgcgaacttg ccgcgcgaac tctcctcacc gacgaggcgg agcgcccgcg
     3421 gcagctcgtc gaacgtgaac gtccgcgtga tcgggacgct gagcgtcccg tccccggcca
     3481 tctgcacgag gcggccgaac gcctgcgggt cggcgtgacc ggtcacgttc gccgcggcga
     3541 ccccgcgttg ggcgagcgcg tccacgtcgg cgccacccac cgtggaggcg atcctcccgc
     3601 cgctcttcac gagctcggtc agcgcgccga acccgtcgcc gacgtgcgcc gcgacgatca
     3661 ggccgtcgac gccctcggga taccgttcga gcatcgccgc cacgacgtcg cccgagtagt
     3721 ccacgatctc gctcgcgccg agcccgcgga tccaggcctc gtcccgatgc aacgccgacg
     3781 cgacgacccg ggcgccgcgg tcggcggcga gctgcaccga gtacgtaccg acgccgcccg
     3841 tcgcgcccac gatcagcatg acgtcgccct cagccgcctc gatcgtctcc acgcagttgc
     3901 tcgccgtgat ggccgcgagc ccgatgcacg ccgaccgctc gaagtcgagg ttcccaggct
     3961 tgtgcacgac gaagccgtcc aggggcacgg ctacgaactc ggcgtacgcg ccgcgccaca
     4021 gatggggctc ggtcgggacg atcccggtga cctcgtcgcc gacggcgaac ccggtcgcgt
     4081 cctccccgag cgcgtcgatc tcgcccgcgt agtccttgcc gatcacgacg gggtaccggt
     4141 gctccatcat gcccttggcc atgcccttgg ccacgaacac gtcgaacccg ttcacggagg
     4201 acgcgcgcac cttgacccga acctcgccgg gccccggctc ggggatcggg acctcgatga
     4261 ccgccgccga agctccctcg tccgccaatc cgaacgcctt catcctgcct ccctcgcctc
     4321 tcgatgggtc gtttccccgg gccagatccg gtccggagcg tagccgaacc tcgaaccgcg
     4381 cccgcatggg ccggtggtgt gtcgttcgtc acgcgagagt tcggaacgtg ccgggggtcg
     4441 tagctgttgg gtacgttcga gggagaagga gcgagccgca tggaagagct gacggcgagg
     4501 gaccggcccg cgcatccggg atcggacgag ctcgagctcc ggctggagga gcacaggacc
     4561 gagctcaccg cgtacgccta ccgcatgctg ggatccgggt tcgaggcgga ggacgccgtg
     4621 caggaggcgt tcctgcgggc gtggaagagc ttcgagcagt tcgagggtcg atcgtcgttg
     4681 cgctcgtggc tgtacagcat cgtgacgaac gtctgtctgg acatgttggg gggcagggag
     4741 cgccgggcac gccccatgga gctcgcgccg gcgagatccg cggacatccc gctgccggac
     4801 ccgctccccg agaacgtgtg ggtcctgccc gtgcccgacg atcgggtggt gcccgagagc
     4861 ggcgatccct ccgaggtgct cgaggcgcgc gagtcggtcc ggctcgcgtt cgtggccgcg
     4921 ctgcaacacc tgccgccgcg gcagcgggcg gtgctgatcc tccgagaggt cctgcgctgg
     4981 aaggcatccg aggtggccga gctcctcgac acctcggtcg cgtcggtgaa cagcgccctg
     5041 caacgcgcgc gcgcgaccat cgagtcactc gagatcgagg acgccgagac cctgcggcct
     5101 gccgacgacg agcaacgcgc gctgctcgac cgctacgtgg atgcgttcga gcggtacgac
     5161 atggattcgc tcacctcgct cctgcgcgag gacgccacct ggtcgatgcc gccgtacgag
     5221 atgtggctgc acacccacga cgacatccgg gcgtggtgcc tgggccctgg gatcggctgc
     5281 cgcggttcgc ggctgatcgc ggtcgaggcg aacggcatgc cggcgttcgg ccagtacaag
     5341 cccgcggagg gagggctcga gccgtggtca ctgcaggtcc tggaggtctc cgacggtcgg
     5401 atcgacggga tcagcttctt cctggacacg gaacgcctgt tcccgcagtt cgggcttccc
     5461 ccacgcctcg acggctgacg cgaccaccgc tccgccgccg gtcagcacct cgagcagacc
     5521 cgccagcctc aggaggtcct cgagctcccc gcaggtcccc cggagctcga cccggtaccc
     5581 gaggcgccgg gcggtcagct ggagccgagc gagcgcgtcg atcgtggtcg cgtccggttc
     5641 ggcaagcgcg ccgacgtcgc acgcgaccgg cccggcgtca cagccctcga gcagcctctc
     5701 cgcgcgctcg cacaacgcgg ggatcgcgtc gcgcgtgacc gggccgctca tgaccaggac
     5761 ggttcctccc ggccccgggc gccaccgccg cgccaccggt tggttcgccg accacgcccg
     5821 ggcctccggc ggggacgccc gttcgttcgc agtcatctcc ggtgagaccg atccggtgcc
     5881 ctgaactcat cggcccgagg cgtggcgccg ccgggccggt agccgttcgg gtcgaccgca
     5941 acctcgtccc agccgagcgc cgcgaccgca cggagcgcgg cgtcccgctc gggatgggcg
     6001 aggagccgat cgacctcgcc ggccggcacc tcgaccgtcg aggaggcgcc gcggtgccgg
     6061 acgcggacca cctcgaaccc gagcgcgcgg acgatcgcct cggcctcgtc gatccgggcc
     6121 agcagctcgg gcgagatgcg gatcccgagc tccacccgcg aggacaggca cgcgagcgcc
     6181 ggcttgtccg acacgcggag gccgagcgtc ctggcgatcg agcgaaccgt cgccttgccg
     6241 accgacccct cgagcagcgg gttgcgtatc ccggcccgtt cccccgccca gatccccggc
     6301 cggaactcgt ccagatcgtc ggcgttcgcg ccggccagca ccgcggtatc ggggatcgcg
     6361 gtccggacga gcgctccgag gacgccgtac agctccgcct tgcagtggta gcaccggagc
     6421 tcgtcgttgc gcgcgtacgc ctcgcgctcg acctcgtggg tccgcaccga ccggtgcgcg
     6481 acaccgagat cggatgcggt tcgggccgcc acctccaact cgcccgccgc atagctcggc
     6541 gagatcgcgg tcaccgcctc gacccgggcg ggcccgagcg cgcgcgcggc gagcgccagc
     6601 accaccgacg agtcgacccc gccggagaac gcgacgaggg ctcggtcgcc gtacccgcgg
     6661 atccgatcct cgagatcgcg gatcacggcg acccgggcgc gttcggcgac cgcgctcatc
     6721 ggtcctcctc gagcagccgg tacgcgagcg ccgtggcctg cgcgtgcacc gaacgcaccg
     6781 gccggccgag cttgcgagcg gcctcgacga cgtcggcgtg ctcgggcttc gcgttcaggg
     6841 tccgtccctc caggaccccg agcttcaccc ggatccgcgg accgccctcg gccagatcga
     6901 tctcgacgat cctgcgatcg agctcgggac gcgacacctc gtggacgcgg accccgagcg
     6961 tcgtcgtggc ctcgaagaac gcccgccgca cctcgtccag ctcctcgcgt tcgcacaggg
     7021 ccgagatcgc gacggccggc cgcccccact tcatcacgat cggcgcggtc cacgcgtcga
     7081 gcgccccggc cgccaagagc gcgtcgatcg cgtcgggcac gagctccggc cccaggtcgt
     7141 ccacgttggc ctcgagcacg agcagccggc ggtcacgggc cgcgggcggc gaggtcgatc
     7201 cgagcagtac ccggacgacg ttcgcgaccg atgccgggtc gcgcgtgccc gcgccgacgc
     7261 cgacccgctc gagcacgagc tctggcatct cggggacggg acggccgagc tcggacagga
     7321 tcgcggccgc ggtcggcgtg acggtctcgg gcaggtccat cccggcgacc gagggccgca
     7381 gggcgaaccc cgacaacagc tcgagcatca ccggcgcggg cgagccgtgc gtcccgccgt
     7441 tcgagggcgg gggcatcggg atcgccgaga cgtcgacgcg ttcgacgccg agcgatgcga
     7501 gcgccgcggc cacgccgacg acgtccagca acgtgtcgtc ctcgccgagc tcctcgagct
     7561 cgaggtcgtc gagcccgcgc ccgtggacct tcgcctcggc gtcgaacagc cgccgcagga
     7621 ccgcggtggc catcgcccga agcccgtcct ggagcgccgc gcgttcgacc gcgtccagca
     7681 gctcccgcgg acggcgccgc tcggtcgacg ggtcgcccgg agccgagagc gcgagcgccc
     7741 gcagaccccg ccggcggacc tccgtggtca cgcattcgac ccggcgaccg aggacggccg
     7801 acaccgcgtc ggagacctcg tgtcgtgacg cgccggcgtc cagcagggcg gccaggagca
     7861 tgtcgcccgc ggcgccgccg acgacgtcga cgtagaggag gttcatcgcg gcgtcgactg
     7921 ccgagcgatc atcgctgcga tcgtgccggc gccgacgccg tcgtccacgt tcacacaggc
     7981 gacgccgggc acgcacgacg acagcatcga caggagcgcg gccaggccgc cgagcgaggc
     8041 gccgtagccg acgctggtcg ggcaggcgat caccgggcac gcagcgatgc cgccgacgac
     8101 gctcgcgagc gcgccctcca tccccgcgac caccacgacc gcgtcggcgg tggcgagcat
     8161 tggctgcgcg gcggcgagac ggtgcagacc cgcgacgccg acgtccgcga cgatctcggt
     8221 ctcggcaccg agcatcgagg cggtgagctc ggcctcctcg gcgaccccga gatccgacgt
     8281 cccggcggtc acgatgagca cggagcccgt ccggggcggc gcgttccgac ggagcgcgac
     8341 gcatcgagcc ctcgggcgtt cgacgatctc gatcgccgtg ccggccgcgg ccgcgcgcac
     8401 cgcgtcgacg tgctcgggcg aggtccgaga gacgatgatc gacccgtcgt tgcccacgag
     8461 gagccgctcg acgatcgagc ggacctcctc gggcgtcttg ccctcggcgt agacgatctc
     8521 gcaggcgccc tggcggagct cgc
//
LOCUS       DATBRK010000078         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1166805, whole genome shotgun sequence.
ACCESSION   DATBRK010000078 DATBRK010000000
VERSION     DATBRK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166805"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /locus_tag="VIH70_02415"
     CDS             <1..1907
                     /locus_tag="VIH70_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8115249.1"
                     /translation="VAVTAFGSIHRAFLTVVATGLVVTALGGIAFTMLGTTRRANLIR
                     FVPYPVVGGFLAGVGWLLVKGAVYVSADALPYLTPIGDLFQASVLQLWGPAFAFGVIL
                     LVATRLVKRPIVIPVVLGIGLVLFAIGMLVTGSSIASARSGHWLLLGPFDSFQSWQPW
                     TARAISGADWSAVAGQWAGILAAVLVATIAILFNISGTEVVLHRDLDTNGELRDAGVV
                     NVISGALGGIPGFHALSASALAERMSVDARVAGLIAAAVPLAAVIFGASVIELVPRMI
                     VGGVLVFLGLAFLVDWVWDKRKVLPPLEYGVVLLIVAAIIARGFLPGVVVGLVLAVVL
                     FAVNYGRIELVREVAFGETYHSNVDRPPAERAALRSMGERVQILRVHGFVFFGSSNGL
                     LERIRKRAEEGPLRFLVIDLRRVTGVDSSAVVAFVKVMHLAEAAGFELVFTGTSEAVR
                     SQLARGGLVASEVVWFEPDLDRGLQRCEEGLLRDAAPQALGATDGRDGRWGAAGMPAG
                     LERYTERESLSDGAVLIRQGESPDDVYVLESGRLRVEMQTPDGTRVRVRSINPGVVVG
                     EVAMYSGDPRTADVLAEGPTVVLRLDRASIDRLEAHDPELAAALHRWLAGVLSERLSD
                     TLREFDALLD"
     gene            1920..>3844
                     /locus_tag="VIH70_02420"
     CDS             1920..>3844
                     /locus_tag="VIH70_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY8115250.1"
                     /translation="MSEERRSQGWLGRLAAGLIIGGVEVVLAVAFAALVFAGAAPVHL
                     GDGIGLYLVAAALTLGFLAWRAGVRGVVGSVQDAATAVLSVLALSTAFKASQIVLLCQ
                     NASVGKCEVPDVFLTVVAATLVVTVSCGVIFLVLGTFRLGNLVRFVPYPVVGGFLAGT
                     GWLLFKGGINIASGVVLHFRTFELLVSPVTLRRYVPALAFGVALYIAVRLVKKPLVIP
                     IVLGVGLALFAIGMLATGSSIDAAKHGRWLLGPFESARLWQPWTLRALTGADWSAVLS
                     QWRGIATAIFVATIAILFNVSGTEVVLHRDLETNQELRDAGVLNVVSGAVGGIPGYHA
                     LSLTALAEQMSVDARVAGLIAAAVPLAGVIFGASLIELIPRMIVGGVLVFLGLAFMVD
                     WVWDKRKVLPPLEYGVVLVILAVIIGRDYLSGVVVGLVMAVVLFAINYGRIELVRDVA
                     FGETYHSNVDRPPAERALLRTMAERVQILRVDGFVFFGSANGMLERIRKRAETNPPRF
                     LVIDLGRVTGVDSSAVVAFVKALHLAEAAGFELVFTGTSDAVRGQLARGGLVASEIVR
                     FEPDLDRGLQRCEEGLLRDEAVAVPESVSILGADALGGMPPGLRAHLERMDLADGTVL
                     IRQGETPGDVYVLETGRL"
BASE COUNT          431 a         1266 c         1446 g          701 t
ORIGIN      
        1 cggtcgcggt caccgcgttc gggagcatcc accgggcctt cctcaccgtc gtcgccaccg
       61 gcctggtcgt gacggccctg ggcgggatcg ccttcacgat gctcgggacc acccggcgcg
      121 cgaacctcat ccggttcgtc ccctatccgg tcgtcggagg cttcctggcc ggcgtcggtt
      181 ggctgctcgt gaagggcgcc gtctacgttt ccgcggacgc gttgccctac ctcacgccga
      241 tcggcgatct cttccaggcc tccgtgctgc agctctgggg cccggcgttc gcgttcgggg
      301 tgatcctgct cgtcgcgacg cgcctcgtga agcggccgat cgtgatcccc gtggtgctcg
      361 ggatcggcct ggtgctgttc gcgatcggga tgctcgtcac agggtcctcg atcgcgagcg
      421 cccgttcggg tcactggctg ctgctcggac cgttcgactc gttccaatcg tggcagccat
      481 ggaccgcccg agcgatctcc ggcgccgact ggtcggcggt ggccggccag tgggccggga
      541 tcctggcggc cgtgttggtg gccacgatcg cgatcctgtt caacatctcg gggaccgagg
      601 tcgtgctgca ccgggatctg gacaccaacg gggagctgcg cgatgcgggg gtcgtgaacg
      661 ttatctccgg cgcgctgggc gggatccccg gcttccacgc cctcagcgcc tcggcgctcg
      721 ccgaacgcat gagcgtggac gcgcgggtgg cgggactgat cgccgccgcg gtgccgctgg
      781 ccgccgtgat cttcggtgcc tcggtgatcg agctagtccc tcggatgatc gtgggaggcg
      841 tgttggtctt cctgggtctc gctttcctgg tcgactgggt ctgggacaag cgcaaggtcc
      901 tgcccccgct cgagtacggg gtcgtcctgc tgatcgtggc ggcgatcatc gcccgcgggt
      961 tcctgccggg tgtggtggtc ggcctggtgc ttgcggtcgt gctgttcgcg gtcaactacg
     1021 gccggatcga gctcgtgcgc gaggtcgcct tcggcgagac ctaccactcc aacgtggacc
     1081 ggccgccggc cgagcgcgcg gcgctccgtt cgatgggcga gcgcgtgcag atcctgcggg
     1141 tgcatgggtt cgtgttcttc ggctcgtcca acggtctgct cgagcggatc cgaaagcgcg
     1201 cggaggaagg cccgctgcga ttcttggtga tcgacctgcg gcgggtcacg ggcgtggact
     1261 cctcggcggt ggtggccttc gtcaaggtga tgcatcttgc cgaggcggcc gggttcgagc
     1321 tggtgttcac cgggacctcc gaggccgtgc gcagccagct cgcgcgcggg ggactggtcg
     1381 cttccgaggt cgtgtggttc gagccggacc tggatcgcgg cttgcaacgc tgcgaggagg
     1441 ggctgctgcg ggacgcggcg ccgcaggccc tcggtgcgac cgacgggcgg gacggccggt
     1501 ggggagcggc cgggatgccg gcgggtctgg agcggtacac agaacgcgaa tcgctgtccg
     1561 acggcgccgt gctgatccgc cagggcgagt cgcccgacga cgtgtacgtg ctggagtccg
     1621 ggcggctccg ggtggagatg cagacgcccg acggcacgcg cgtgcgggtg cgttcgatca
     1681 accccggcgt ggtcgtcggc gaggtcgcga tgtactcggg ggacccgcgc accgccgacg
     1741 tgctggccga gggaccgacc gtggtcctgc ggctcgaccg tgcctcgatc gatcggctcg
     1801 aggcgcacga tcccgagctg gcagcggcgc tgcaccggtg gctcgcgggt gtgctctcgg
     1861 aacggttgag cgacaccctg cgggagttcg acgccttgct cgactagacg ggagggacgg
     1921 tgtccgagga gcgacggtcg cagggctggt tggggcggct cgccgccggg ctgatcatcg
     1981 gcggcgtcga ggtggtcctc gcggtcgcgt tcgccgccct cgtgttcgcc ggcgcggccc
     2041 ccgtccacct gggcgacggc atcggcctgt acctcgtcgc ggccgcgctc acgctcgggt
     2101 tcctggcttg gcgtgcgggc gtgcggggcg tggtgggcag cgtgcaggac gccgcgacgg
     2161 ccgtgctctc ggtcctggcg ctgagcacgg cgttcaaggc ctcccagatc gtgctgctct
     2221 gccagaacgc gtccgtcggc aagtgcgagg ttcccgacgt cttcctcacc gtcgtggccg
     2281 cgacactggt ggtgacggtc tcgtgcggcg tcatcttcct cgtgctcggg accttccggc
     2341 tcggcaacct ggtccgcttc gtcccgtatc cggtcgtcgg agggttcctg gccggcaccg
     2401 gatggctgct cttcaagggc ggcatcaaca tcgcctccgg tgtggtgctg catttccgca
     2461 cgttcgagct cctggtgagc ccagtgacgc tgaggcgata cgtgcccgcg cttgctttcg
     2521 gggtggcgct gtacatcgcc gtccggctgg tgaagaagcc cctggtcatc ccgatcgtgc
     2581 tcggggtcgg gctcgcgctg ttcgcgatcg gcatgctcgc gacgggctcc tcgatcgatg
     2641 ccgccaagca cgggcgatgg ctgctcgggc cgttcgagtc ggcccggctg tggcagccct
     2701 ggacgctccg ggcgctcact ggcgccgact ggtcggcggt gctgagccag tggcgtggga
     2761 tcgccacggc gatcttcgtg gccacgatcg cgatcctgtt caacgtctcc gggaccgagg
     2821 tcgttctgca ccgggacctg gaaacgaatc aggagctccg cgacgccgga gtgctcaacg
     2881 tggtctccgg cgcggtgggc gggatccccg gctaccacgc cctcagcctg accgcgctcg
     2941 ccgagcaaat gagcgtggac gcccgggtgg cgggcctgat cgcggcggcg gtgcccctgg
     3001 ccggggtgat ctttggcgcc tcgctgatcg agctgatccc gcggatgatc gtgggcggcg
     3061 tgctggtgtt cctgggcctg gcgttcatgg tcgactgggt ctgggacaag cgcaaggtgc
     3121 tgcccccgct cgagtacggg gtggtcctgg tgatcttggc ggtgatcatc ggccgggatt
     3181 acctgtccgg cgtggtggtc ggcctggtga tggcggtcgt gctgttcgcg atcaactacg
     3241 gccgcatcga gctggtgcgc gacgtcgcct tcggggagac ctaccactcc aacgtggacc
     3301 gcccgccggc cgagcgggcg ctgctgcgca cgatggccga gcgggtccag atcctgcggg
     3361 tcgacggctt cgtgttcttc ggctcggcca acggaatgct cgagcggatc cgaaagcgag
     3421 cggaaaccaa tccgccgcgg ttcctggtga tcgacctcgg ccgcgtcacc ggcgtggatt
     3481 cctcggccgt ggtggcgttc gtcaaggcgc tgcatctggc cgaggcggcc gggttcgagc
     3541 ttgtgttcac cgggacctcc gacgcggtcc gcggccagct ggcgcgcggg ggcctggtcg
     3601 cctcggagat cgtgcggttt gagccggacc tggaccgcgg cctccagcgg tgcgaggagg
     3661 ggctgctgcg ggacgaggcg gtcgcggtgc ccgaatccgt ctcgatcctt ggcgcggacg
     3721 cgctcggcgg catgccgccc ggcctgcgcg cccacctcga acgcatggac ctcgccgacg
     3781 gcaccgtgct gatccgccag ggcgagacgc ccggcgacgt ctacgtgctg gagaccggcc
     3841 gcct
//
LOCUS       DATBRK010000079         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2463037, whole genome shotgun sequence.
ACCESSION   DATBRK010000079 DATBRK010000000
VERSION     DATBRK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2463037"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VIH70_02425"
     CDS             complement(<1..291)
                     /locus_tag="VIH70_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115251.1"
                     /translation="MRKFLLLTGAALFALALTSSSALAAVNVKRLPVADFGNPDAASV
                     TLFGGNFSGLGSVPAIATLEVTGTVDYDCANPQGHLSPGQNPVAAQPGLAQQD"
     gene            complement(794..2464)
                     /gene="ilvD"
                     /locus_tag="VIH70_02430"
     CDS             complement(794..2464)
                     /gene="ilvD"
                     /locus_tag="VIH70_02430"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY8115252.1"
                     /translation="MVDRPRSHMVTDGPERAPARAMLRAVGFSEEDFGKPQVGVASSW
                     NEVTPCNYHLDKLAALAKEGVRQGDAVPIEFTTIAISDGIAMGHEGMKASLISRDVIA
                     DSVELVMHAERLDGLVGIAGCDKSEPGMLMAMARLNLPSVYLYGGTILAGTYGGRDIT
                     IQDVFEAVGAYAKGSIDDAELLGIERAACPTTGSCAGMYTANTMAAAGEALGMSLPGS
                     ASPPAVDYRREVFARESGLALTKLLESGLRPRDILTPDAFTNAIAVVMALAGSTNAVL
                     HLLAIAHEAGVHLELDDFDRISRVVPHLVDVRPAGRFVMQDLDRVGGVPVVMKELLDA
                     GLLNGDAITVTGKTIAENLEQVGPPAPDGTVVRPFAEPIHPEGGTAILYGSLAPEGSV
                     MKIAGAASLQFSGRARPFDSEQEAFQAVTSGSIVAGDVIVIRYEGPRGSPGMPEMLSV
                     TAAVAGAGLGAEVGLITDGRFSGASKGYSVGHVSPEAFVGGPIALVREGDPIAIDAAN
                     RRIDLQVDDDELQRRRTAWMQPAPRYESGALAKYARLVGSASRGAVTW"
     gene            2586..>3430
                     /locus_tag="VIH70_02435"
     CDS             2586..>3430
                     /locus_tag="VIH70_02435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8115253.1"
                     /translation="MRRADQPRPAERSWWLEEALAQPEFAGPEAPPLERDTTADVVIL
                     GGGYTGMWTAWFLKERRPDLDVVLLESDICGGGPSGRNGGFCNGWWEKIRDVRETYGD
                     ADAMELLTTCGRAPTEIGTWCRANGVDAWFRHGGDLAVATNERHEGSWQGSLEDARAL
                     GVEDEYRILTAGEVQGRCASPRFGSGVLIADAATVHPARLARGLRRLLIENGVRIHER
                     TTVTRLGMGARVTAETQGGSVRAADAVVGLGAWATWWKAFKPRLTLRGSYMVVTAPAP
                     DRIQAL"
BASE COUNT          525 a         1228 c         1157 g          520 t
ORIGIN      
        1 atcttgctga gcgagcccgg gctgcgccgc cacaggattc tggccggggg acagatgacc
       61 ctgcgggttc gcgcaatcat agtcaaccgt tcccgtgacc tccagggtgg cgatcgctgg
      121 gacacttccc agtccgctga aattgccacc aaacagggtc accgacgctg cgtccgggtt
      181 gccgaagtca gccacgggca gcctcttcac gttgactgct gccagtgcac tcgaagacgt
      241 gagggcaaga gcgaacaatg ccgctccggt tagcaatagg aacttcctca tgatggctcc
      301 tttcgaagag ggatcgatca gccgagccag cccgacggct ccactccact ctttgcggtc
      361 agcgagatcg aagggcccgg gtgttcggcc cttcttgaaa ccttcgcatc gtgcactcct
      421 ccttattcct ggatccgccc gctcctcggc gcctcccaac cccacctcct gatcgctcgc
      481 gtccgtagag aagcggctcg acttccagtc tcagccgatg cgcgatctcc tcgcaccagc
      541 atcaagacgg accttcgcgc cacttcgacc gctccggcga cacgaacgaa cgcacaaagg
      601 gacctatccg gaacctgatg ctgccccttt tgggcgcccg ggtgggcctc agaagggtcc
      661 taggatgccc ttagcgacgg gctgtgcccg cgtactcact cccaaggtca gctggatgtc
      721 gtggatcagc gtcttgacgg tccgctccgg gagccggatg ttcctggcga ccgtccgccg
      781 tcggccaaat cgctcaccag gtgacggccc ccctggaggc cgaaccgacc agtcgagcgt
      841 acttggccag cgccccgctc tcgtagcggg gcgctggctg catccacgcg gtccgccgtc
      901 gctgcagctc gtcgtcgtcc acctgcaggt cgatccggcg gttggccgcg tcgatcgcga
      961 tcggatcgcc ctcgcgcacc agcgcgatag ggccgccgac gaatgcctcc ggcgagacgt
     1021 ggccgacgct gtaccccttc gaggctccgc tgaaccgccc gtcggtgatc agacccacct
     1081 cggccccaag gcccgcaccc gcgaccgccg cggttaccga gagcatctcc ggcatccccg
     1141 gagaccccct cgggccctcg taccggatca cgatgacgtc gcccgccacg atcgaaccgc
     1201 tcgtgaccgc ctggaacgcc tcctgctcgg agtcgaaggg ccgcgcccgc ccgctgaact
     1261 gaagcgacgc ggccccggcg atcttcatca cggagccctc gggcgccagc gaaccgtaga
     1321 ggatcgccgt gccgccctcg gggtggatgg gctccgcgaa cggccggacg accgtcccgt
     1381 cgggtgcggg tggcccaacc tgctcgaggt tctccgctat cgtcttgccc gtcacggtga
     1441 tcgcgtcgcc gttcagcagg ccggcgtcca ggagctcctt catcacgacc ggcaccccgc
     1501 cgacgcggtc gagatcctgc atcacgaacc tgcccgccgg ccgcacgtcg acgaggtggg
     1561 gcaccaccct gctgatgcgg tcgaagtcgt cgagctcgag gtggacgccc gcctcgtgcg
     1621 cgatcgcgag cagatgcagc accgcgttcg tcgaaccggc cagcgccatc acgacggcga
     1681 tcgcgttcgt gaaggcgtcc ggggtcagga tgtccctggg ccggagaccg gactcgagca
     1741 gcttggtcag agcgaggccc gactcccgcg cgaacacctc gcgccggtag tcgacggccg
     1801 gcgggctcgc gctgcccggc aacgacatgc cgagcgcctc ccccgccgcg gccatcgtgt
     1861 tcgccgtgta catgccggcg cacgagccgg tggtcgggca cgccgcgcgc tcgatgccga
     1921 ggagctcggc gtcgtcgatc gagcccttcg cgtacgcccc caccgcctcg aacacgtcct
     1981 ggatcgtgat gtctcggccc ccgtacgtcc cggcgaggat cgtgcccccg tagaggtaga
     2041 ccgagggcag gttgagccgg gccatcgcca tgagcatccc cggctcggac ttgtcgcacc
     2101 ccgcgatgcc cacgagcccg tcgagccgct cggcgtgcat cacgagctcc accgagtccg
     2161 cgatcacatc gcggctgatc agcgatgcct tcatcccctc gtgtcccatc gcgatgccgt
     2221 cggagatggc gatcgtcgtg aactcgatcg ggacggcgtc gccctggcgc accccctcct
     2281 tcgcgagcgc ggcgagcttg tccaggtggt agttgcaggg ggtgacctcg ttccacgacg
     2341 aggcgacgcc cacctgcggc ttgccgaagt cctcctccga gaagccgacc gcccgcagca
     2401 tcgcgcgggc cggtgcgcgt tcgggcccgt ccgtgaccat gtgagaacgt gggcgatcga
     2461 ccatggcgat gtcctcctga cgagctagcc atcagacctg gccaaagaga catggaagga
     2521 tgggctggca cgataccgca gccccgtcgc gggcaggccg cgacctcgga ggaaacggaa
     2581 ggacgatgcg gcgcgccgac cagccccgcc ccgccgagcg ctcgtggtgg ctggaggagg
     2641 cgctcgcgca accggagttc gcggggccgg aggcgccacc actcgaacgc gacacgaccg
     2701 cggacgtcgt gatcctcggc ggcgggtaca cgggcatgtg gaccgcgtgg ttcctgaagg
     2761 agcggcgacc ggacctcgac gtcgtgctgc tcgaatccga catctgcgga ggcggcccga
     2821 gcggccgcaa cggcgggttc tgcaacgggt ggtgggagaa gatccgcgac gtccgcgaga
     2881 cctatggcga cgccgacgcg atggagctgc tgacgacgtg cggtcgcgcg cccaccgaga
     2941 tcggcacctg gtgccgggcg aacggggtcg acgcgtggtt ccgacacggt ggcgacctcg
     3001 ccgtcgccac gaacgagcgg cacgaggggt cgtggcaggg ttcgctggag gacgctcggg
     3061 cgctgggcgt cgaggacgag taccggatcc tgaccgccgg cgaggttcag gggcggtgcg
     3121 cgtccccgcg gttcggcagt ggggtgctga tcgccgacgc cgcgaccgtt catcccgccc
     3181 ggctcgctcg gggcctgcgg cgcctgctga tcgagaacgg cgtccggatc cacgaacgca
     3241 cgacggtcac gcggctcggc atgggtgcgc gggtgacggc cgagacgcag ggtggctcgg
     3301 tgcgggccgc cgacgccgtg gtcggtctcg gcgcgtgggc gacctggtgg aaggccttca
     3361 agccgcggct cacgctccgg gggtcctaca tggtcgtcac cgcgccggcc ccggaccgga
     3421 tccaggcgct
//
LOCUS       DATBRK010000080         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127 k141_9962,
            whole genome shotgun sequence.
ACCESSION   DATBRK010000080 DATBRK010000000
VERSION     DATBRK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9962"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /gene="nusA"
                     /locus_tag="VIH70_02440"
     CDS             complement(<1..911)
                     /gene="nusA"
                     /locus_tag="VIH70_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY8115254.1"
                     /translation="MNQEMIAALRELEREKGIAFETILEGLEEAMASAYKSWWKQQHP
                     ELDEETFGVRAKIDPENGDLRVWIQQLERIEHEDEEGEITVETKVLSEQEVPVTDEFK
                     GRIGAQTAKQVIFQKLRDAEREMTYEEFAGREGDVVTGIVQQSERRYTLLDLGKVEAL
                     LPQAEQVPSEPYRNGERLKGYITEVRKGTKGPQIVVSRTHPGLLKKLFELEVPEIAEG
                     IVEIKAVAREPGHRSKIAVSSNEPAVDPVGACVGPKGSRVRNVVTELRGEKIDVVPWS
                     ADPATFVANALQPAKVREARVDPETQTA"
     gene            complement(908..1399)
                     /locus_tag="VIH70_02445"
     CDS             complement(908..1399)
                     /locus_tag="VIH70_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY8115255.1"
                     /translation="MRENGVGDLVRPVVEAAGLELWEASFGREDGRLVLRVMVDRAGG
                     EGKDGGVDLDTIAAVSQRLSRRLDLEGFERDRPYELEVSSPGLERVLREPRHFERSVG
                     QRVRMKTVEPVDGRRVHEGALVSADAEAIVIASDGGELRVPYDGIASARTVFEWKKAG
                     SGS"
     gene            complement(1516..1791)
                     /locus_tag="VIH70_02450"
     CDS             complement(1516..1791)
                     /locus_tag="VIH70_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115256.1"
                     /translation="MRAPSGLREFVKLRPEVEAVVQHVGLRTWDLVLIDVTGLWVRDE
                     FENEEQVEAVCNELGIRLHRAWDDPRLVRRMAARDHWNTPDGQRRAL"
     gene            complement(1824..>2345)
                     /locus_tag="VIH70_02455"
     CDS             complement(1824..>2345)
                     /locus_tag="VIH70_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8115257.1"
                     /translation="GELRIGRSIVVGHIYQLGTRYSAPLRATFVDEDGSERLYQMGSH
                     GIGISRVLAAAVEQFHDEMGLRLPKALAPFEVVVVLANRDHERVVAEAERVYRELLEA
                     GADAALDDRDQTAGVKFADADLIGCPVQVVIGKRGVEAGTADLKLRASGERSQAPLAE
                     AAGAAMDLLASAP"
BASE COUNT          321 a          915 c          718 g          391 t
ORIGIN      
        1 gcggtctggg tctcggggtc cacgcgcgcc tcccgaacct tcgccggctg cagcgcgttc
       61 gcgacgaagg tcgcggggtc cgcgctccag ggcacgacgt cgatcttctc gccacgcagc
      121 tcggtcacca cgttgcggac acgcgaaccc ttcggcccga cgcacgcacc caccgggtcg
      181 accgccggct cgttggacga gaccgcgatc ttcgaacggt gccccggctc gcgtgccacg
      241 gccttgatct cgacgatccc ctcggcgatc tcgggcacct cgagctcgaa cagcttcttg
      301 agcagccccg ggtgggtccg cgacacgacg atctgcggac ccttggtgcc cttgcgcacc
      361 tcggtgatgt agcccttgag ccgttcgccg ttgcggtagg gctcgctggg cacctgctcg
      421 gcctgcggga ggagcgcctc caccttgccg aggtcgagca gcgtgtaccg gcgctccgac
      481 tgctggacga tgccggtcac gacgtccccc tcgcgcccgg cgaactcctc gtacgtcatc
      541 tcgcgttcgg cgtcgcggag cttctggaag atcacctgct tcgcggtctg cgcgccgatg
      601 cgccccttga actcgtcggt gaccgggacc tcctgctcgg acaggacctt ggtctcgacg
      661 gtgatctcgc cctcttcgtc ctcgtgctcg atccgctcga gctgctggat ccagacgcgc
      721 aggtcgccgt tctccgggtc gatcttcgcg cgcacgccga aggtctcctc gtcgagctcc
      781 gggtgctgct gcttccacca cgacttgtag gcggacgcca tcgcctcctc gaggccctcg
      841 aggatcgtct cgaacgcgat ccccttctcg cgctcgagct cgcgaagcgc cgcgatcatc
      901 tcttggttca tgacccgctc cctgccttct tccactcgaa gacggtcctc gccgaagcga
      961 tcccgtcgta ggggacgcgc agctctccac cgtcggacgc gatgacgatc gcttccgcgt
     1021 cggccgagac cagcgcgccc tcgtggaccc tgcgaccgtc caccggctcc accgtcttca
     1081 tcctcacccg ttgcccgacg cttcgttcga agtgccgggg ttcgcggagc acccgctcga
     1141 gcccgggcga gctcacctcg agctcgtacg ggcgatcccg ctcgaacccc tcgaggtcga
     1201 gccgtcgtga caggcgctga gacaccgccg cgatcgtgtc caggtcgaca cccccgtcct
     1261 tcccttcccc gcccgcccgg tccaccatca cccggaggac caggcgaccg tcttcccggc
     1321 cgaaggaggc ctcccagagc tccagtccgg cagcctcgac gaccggccgg accagatcgc
     1381 cgaccccgtt ctcgcgcacc cgaacgaccc cttcgaaaga aaaagtgggc gcgagcccac
     1441 ttccgggtga caccgcttgc gtttctgctg gtcagtatac ccgacgctgc tgcctccgag
     1501 gcccggaggc gtgcctcaga gcgcccggcg ctgcccgtca ggggtgttcc agtggtctcg
     1561 cgcggccatc cgacggacga gacgcgggtc gtcccaggct cgatgcagcc ggatccccag
     1621 ctcgttgcac acggcctcga cctgctcctc gttctcgaac tcgtcccgca cccacaggcc
     1681 cgtcacgtcg atgagcacga ggtcccacgt ccgaaggccg acgtgctgca cgacggcctc
     1741 cacctccggt cgcaacttga cgaactcgcg aagaccgctc ggtgccctca ccgaggcccc
     1801 ctcgtcccgt ccaccgttcc accctagggg gcactcgcga gcaggtccat cgcggcgccc
     1861 gcggcctcgg ccaggggagc ctgcgatcgc tcaccgctcg cccgcagctt gaggtcggcc
     1921 gtgccggcct cgacgccccg cttcccgatc acgacctgga ccgggcagcc gatcaggtcg
     1981 gcgtcggcga acttcacccc tgcggtctgg tcgcggtcgt cgagcgcagc gtccgcgccg
     2041 gcctcgagca gctcccggta cacgcgctcg gcctcggcga ccacgcgttc gtggtcccgg
     2101 ttggcgagca ccaccaccac ctcgaacggc gcgagcgcct tcggcagtcg cagtcccatc
     2161 tcgtcgtgga actgctcgac ggcggccgcg aggacgcgtg agatgccgat cccgtggctc
     2221 cccatctggt agagccgctc cgaaccgtct tcgtcgacga acgtcgcccg cagcggggcc
     2281 gagtaccgcg tcccgagctg atagatgtgg cccacgacga tcgaacggcc gatccggagc
     2341 tcgcc
//
LOCUS       DATBRK010000081         3812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_296672, whole genome shotgun sequence.
ACCESSION   DATBRK010000081 DATBRK010000000
VERSION     DATBRK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296672"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VIH70_02460"
     CDS             complement(<1..171)
                     /locus_tag="VIH70_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY8115258.1"
                     /translation="MTLDAELLEILVCPNDRGELDYQQDPEILVCRTCGYRYPVRDGI
                     PVMLIDEAEKPAT"
     gene            complement(168..1532)
                     /gene="manB"
                     /locus_tag="VIH70_02465"
     CDS             complement(168..1532)
                     /gene="manB"
                     /locus_tag="VIH70_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871837.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY8115259.1"
                     /translation="MTVSPHRDLGRLFKAYDVRGVVPDELDEDVARSIGAAFAEWSGV
                     EAVAIGRDCRLSSHSLAAALGEGITSRGTDVIDIGLASTDLLYFTSGSRSVAGIMLTA
                     SHNPPEYNGMKFCLPGARPVGQDTGLGEIRNLTERHAFAPVAARGTARVEEMLEAYVE
                     HVLSFVDVSAMRPLTVVADTANGMGGLVVPPVFERLPMTLVHLYPELDGTFPNHPADP
                     MDPENQRDLKRELLGRKADVGLAFDGDADRVFLVDDLADGVSGSEVTALVATAMLERH
                     PGAKIVYNLICSWAVPEVIREQGGEPIRTRVGHSFIKQEMAETGAVFGGEHSGHYYFR
                     ENYRADSGLIAAVVVLERLSRSSEPLSSVLTPFRRYHDSGEINSRVDDQDAKIEELAA
                     AYRDGRPDRTDGLTVEFEDWWFNVRPSNTEPLLRLNVEARTEELLEDKTSELLELIRE
                     GGPT"
     gene            complement(1557..1748)
                     /locus_tag="VIH70_02470"
     CDS             complement(1557..1748)
                     /locus_tag="VIH70_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115260.1"
                     /translation="MNDKHEGSCPVCHQGDLITITMNVSANDLSFTTCHMCESKWWYR
                     DGESVPLRSVIGSVLQPRT"
     gene            complement(1907..2116)
                     /locus_tag="VIH70_02475"
     CDS             complement(1907..2116)
                     /locus_tag="VIH70_02475"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HEY8115261.1"
                     /translation="MRTCSRIGCSSPAATTALVRYEAREIELRPLVDEPDPHLVDLCS
                     DHGARLSPPRGWTIAGQRHRQAVSA"
     gene            complement(2370..3425)
                     /locus_tag="VIH70_02480"
     CDS             complement(2370..3425)
                     /locus_tag="VIH70_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY8115262.1"
                     /translation="MARARHRRGGRWVIAAVTLGVLAVVTAGTAFAAYQYDASTSDRI
                     LPGVSVGGIDVGGMKRDEAVRTLRELAEQTLYSELVVQAAGHSWNVTPASLGMTADVE
                     GAVARALAVADDLSLVSRVYHRVREVPVDVSLGLPFDEHRREVKAFVQQAFDEVAVPA
                     VDARFALVDGELVTNRSHSGRELAIGAATDQILEALGRRTDAVEVPVRTVEPSVTTAA
                     LGQSIVVDLSENELYLYDGLKVVADYPVATAAAGYTTPVGTWEVVDKRENPTWYNPAQ
                     DTWGADLPPTIAPGPGNPLGTRAIYLNAPGIRIHGTYSSSSIGTYASHGCIRMYISDS
                     EELFGLVGVGTRVIIKP"
     gene            complement(3562..>3812)
                     /locus_tag="VIH70_02485"
     CDS             complement(3562..>3812)
                     /locus_tag="VIH70_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115263.1"
                     /translation="EPAVVTLSFLSPGPAEQISVNVPPRSTVEAPEEFLRVAPEAGVR
                     AEAISGTFIPAAASYSLGRDGFATYAVALGIEIPEGVL"
BASE COUNT          590 a         1317 c         1331 g          574 t
ORIGIN      
        1 ggtcgcgggc ttctcggcct cgtcgatcag catgacgggg atgccgtcgc gcacggggta
       61 ccggtacccg cacgtccggc acacgaggat ctcgggatcc tgctggtagt cgagctcgcc
      121 ccgatcgttc gggcacacca ggatctcgag cagctcggcg tcgagtgtca cgtcggtcct
      181 ccttcgcgga tcagctccag gagctcggag gtcttgtcct ccaggagctc ctcggtcctg
      241 gcctccacgt tcagccgcag gagcggttcg gtgttactgg gccgaacgtt gaaccaccag
      301 tcctcgaact ctaccgtcag cccatccgtg cggtccgggc ggccgtctcg gtacgcggcg
      361 gcgagctcct cgatcttggc gtcttggtcg tctacgcggg agttgatctc acccgagtcg
      421 tggtaccggc ggaacggcgt cagcaccgac gacagcggct cggacgagcg cgacagccgc
      481 tcgagcacca cgaccgccgc gatcaatccc gagtccgctc ggtagttctc gcggaagtag
      541 tagtggccgg agtgctcgcc gccgaagacg gccccggtct cggccatctc ctgcttgatg
      601 aacgagtgac cgacgcgcgt tcggatcggt tcacccccct gctcgcggat cacctcgggc
      661 acggcccagg agcagatcag gttgtagacg atcttcgcgc cggggtgccg ctcgagcatc
      721 gcggtcgcca ccagcgcggt gacctccgag ccgctcaccc cgtccgcgag atcgtcgacc
      781 aggaacacgc ggtccgcgtc gccgtcgaac gccaacccga cgtcggcctt gcggccgagg
      841 agctcccgct tgaggtcccg ctggttctcg gggtccatcg ggtcggcggg atggttcggg
      901 aacgtgccgt cgagctcggg gtacagatgc accagggtca tcgggaggcg ctcgaacacc
      961 ggcgggacca ccagcccccc catgccgttg gccgtgtcgg ccaccacggt gagcggtcgc
     1021 atcgccgaga cgtcgacgaa cgagagcacg tgctcgacgt acgcctcgag catctcctcc
     1081 acgcgcgcgg tgcccctggc cgcgacgggc gcgaacgcgt ggcgctcggt caggttgcgg
     1141 atctcgccca ggccggtgtc ctgcccgacg ggccgggcgc cggggaggca gaacttcatc
     1201 ccgttgtact cgggagggtt gtggctcgcc gtcagcatga tgcccgcgac cgaacgcgag
     1261 ccggacgtga agtacaggag atcggtcgag gcgagcccga tgtcgatgac gtcggtccct
     1321 cgcgatgtga tgccctcgcc gagcgcggcg gcgagcgagt gggacgagag gcggcagtcc
     1381 ctcccgatcg cgacggcctc gaccccggac cattcggcga acgcggcccc gatcgagcgc
     1441 gcgacatcct cgtcgagctc gtcggggacg acgccgcgaa cgtcgtacgc cttgaagagg
     1501 cgtccgagat ctcggtgcgg cgacacggtc accgcatcgt actgaacccc ctgccgtcag
     1561 gttcggggct gcagcaccga gccgatgacc gagcggagcg ggacgctctc gccgtcccgg
     1621 taccaccact tcgactcgca catgtggcag gtggtgaagg acagatcgtt ggcgctcacg
     1681 ttcatcgtga tcgtgatcag gtcgccttgg tgacacacgg ggcaggagcc ttcgtgcttg
     1741 tcgttcatgt acgtatcctc ggccgcgacg agcctcggag cacatagccc gacgcgctca
     1801 actctggggg tcagcgcggg ctccagccgc atcctccgcg agagggtccg ggcgttcgtc
     1861 cgggggatgg tcgggtacgc cgaacggcta cacgccgcgc gcgggctcag gccgagactg
     1921 cctgtcggtg cctttggccc gcgatcgtcc acccacgcgg gggactgagc cgggccccgt
     1981 ggtccgagca cagatcgacg agatgcgggt cgggctcgtc gacgaggggc cgcagttcga
     2041 tctcgcgcgc ctcgtagcgg accagcgccg tcgtggccgc gggggacgag cagccgatcc
     2101 tcgaacaggt tcgcacgaag ggaatgctag gtcacacgga tgtgatttca agcatgggcc
     2161 cgagctcggc ggggcgcgcc tggaggcgca gcgtgggagg cgccgtgtcg ggggcgagca
     2221 cgcaccgcaa cgagatgtac tccgcggtgg ccgccgggtc gcccctttgg tctccacgga
     2281 gtacccgata acgatatccc ggggtcgagc gttcgggcaa gaggtggatc gcccgggcgg
     2341 ggagaaagag tgacaggaac ggccggcggt cagggcttga tgatgacgcg agtgcccacg
     2401 ccgacgaggc cgaacagctc ctccgaatcc gagatgtaca tccggatgca gccgtgcgac
     2461 gcgtacgtgc cgatcgacga cgagctgtag gtcccgtgga tgcggatccc tggggcgttg
     2521 aggtagatcg ccctggtgcc cagcgggttg cccggaccgg gcgcgatcgt cggcggcagg
     2581 tcggcgcccc acgtgtcttg tgccgggttg taccaggtcg ggttctcgcg cttgtccacg
     2641 acctcccacg tcccgacggg cgtggtgtag ccggcggcgg cggtcgcgac cgggtaatcc
     2701 gccaccacct tgagcccgtc atacaagtac agctcgttct cggagaggtc gacgacgatc
     2761 gactgaccga gcgcagcggt cgtgaccgag ggctccaccg ttcggaccgg gacctcgacc
     2821 gcatcggtcc gccggccgag ggcctcgagg atctggtcgg tggccgcgcc gatcgccagc
     2881 tcccgaccgc tgtgcgaccg attcgtcacg agctcgccgt cgacgagcgc gaaccgggcg
     2941 tcgaccgcgg gcaccgcaac ctcgtcgaaa gcctgctgca cgaaggcctt gacctcgcgc
     3001 cgatgctcgt cgaacggcag gccgagagac acgtcgaccg ggacctcgcg cacgcgatgg
     3061 tagacgcgcg acacgagcga cagatcgtcc gcgaccgcga gcgccctcgc gaccgcacct
     3121 tcgacgtcgg ccgtcatccc gagcgaggcc ggcgtgacgt tccagctatg gccggcggcc
     3181 tgcacgacca gctcgctgta gagcgtctgc tcggcgagct cgcgcagggt ccggacggcc
     3241 tcgtcgcgct tcatgccgcc gacgtcgata ccacccaccg acacgcccgg gaggatccgg
     3301 tccgaggtcg aggcgtcgta ctggtaggcc gcgaacgcgg tccccgccgt cacgaccgcc
     3361 agcacgccga gcgtcaccgc ggcgatcacc catcgtccgc cccgccgatg cctcgctcgc
     3421 gccacccgaa ccccgttctc gtcgaaaccg aacctcgcgg cggttgccgc gaggcacata
     3481 atcctatccg gcgtgccgag cgtccagacc ggcccttctc cgcatacagg acgggcgcgg
     3541 gacgggcata cagggacggg cctacaggac gccttcgggg atctcgatcc cgagcgcgac
     3601 cgcgtaggtc gcgaacccgt cgcgaccgag agagtaggac gcggccgccg gtatgaaggt
     3661 tcccgagatc gcctccgccc tcaccccggc ctcgggggcg acccggagga actcctcggg
     3721 cgcctccacg gtcgagcgcg gcggcacgtt gacgctgatc tgctcggcgg gccccgggga
     3781 caggaagctg agcgtgacga ccgcgggctc ga
//
LOCUS       DATBRK010000082         9010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2321004, whole genome shotgun sequence.
ACCESSION   DATBRK010000082 DATBRK010000000
VERSION     DATBRK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321004"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VIH70_02490"
     CDS             <1..800
                     /locus_tag="VIH70_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8115264.1"
                     /translation="YMGEEDTIRQAPNVARMQLLGAEVVTVTSGSRTLKDALNEALRD
                     WAASVVSTHYVLGSVAGPHPFPALVRDLQRVIGDEARVQLGEREGRDPDMVVACVGGG
                     SNAIGIFEAFLPEPSVGLVGVEAGGTGDGLGEHCRSLTLGEPGVLHGSYSYLLQDEQG
                     QVVPTHSISAGLDYPGVGPEHAFLKDAGRATYVSVTDGEALEGFRALATLEGILPALE
                     PAHAIGWLLRRPLPEGSLVLVNLSGRGDKDLETVRAASGPVPGAPSG"
     gene            793..1563
                     /gene="trpA"
                     /locus_tag="VIH70_02495"
     CDS             793..1563
                     /gene="trpA"
                     /locus_tag="VIH70_02495"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY8115265.1"
                     /translation="MADLEPALRSRLEAGGRAFVPYVTGGLAGVDAELLRGLEEAGAD
                     AIEVGIPFSDPVMDGPVIQEASRRALGEGATPSSVLGSIKEAGLEVPVAVMTYANPVW
                     HAGHGAFLDTCVDAGVTGVIVPDLPVDEARDWIAACRRASVAPVFLAAPGTSDDRLRA
                     IGDASRGFVYCVATYGVTGARDRLAETAAELVGRLRPLASLPLLVGVGIAEPPHAAEA
                     CTFADGVVVGSALVRPLLDGDTATAIGLARAFRAATPP"
     gene            1844..3184
                     /locus_tag="VIH70_02500"
     CDS             1844..3184
                     /locus_tag="VIH70_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8115266.1"
                     /translation="MKNRKYFAMLALLGALMLVAAACGNDEGGETGGGGGTSATGGTV
                     DCASDEFGCVEIAAGDPIQLASLLSISGDTAFLGTDSNHGVELAIDHLDNKFDGTPGQ
                     LLGHDVTLQQEDDGCSAEGGQAGATALAANPDIVAVIGTSCSSAALGVADTILGDKGI
                     LLFSPSNTNPGLTSEEAHQPFYARTAHNDKIQGAIVADFVFTKLGIDTAATINDESPY
                     ADGLAAAFRDNFEALGGTITDTEQVNSSDTDLGPVFTNIAATDPGVVYGPNFNPVCAL
                     SYTQGSDILAKGTVQIGSDGCLESSFLDTVGAPIKDYTYYASSPDVSAYQSGAFYKNE
                     YLPAYTDQFGKPTSVFNAHAYDATNVLFDAIEAVAVDDGSGNLTISRTALKDAVLATS
                     GYQGLTGTITCTPLGDCATDVTIGVFEYDHWPVEGGKDPTDPVFSETKTLADVI"
     gene            3266..4366
                     /locus_tag="VIH70_02505"
     CDS             3266..4366
                     /locus_tag="VIH70_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8115267.1"
                     /translation="MATATDEQVRGAELGLLERLPWRRIILVVLSVVILAVVAIGAYK
                     TLTLPADERYTATTWKNFIVLGLAQGAIIGLIALGYTLVYGILRMINFAHGEVFMFGA
                     FGAFFFSDAWEQSGFLNTNPWLSMALLLIIAMLTSMSVAVLLERIAYRPLRNAPRLVP
                     LITAIGASLFLQNTARGFFGAQSHGFGVPEVLDGTWTILGVEVDRIQVVVLVTVIVAV
                     TVLSLFVSKTKTGKSMRAVAADSEIASLMGIRVDRVIVTTFAIGGLLAGIAAVLFSIT
                     FESVSFTMGVRPGIAAFTAAVLGGIGSISGAALGGVLLGLLQSVGPALILTGYGIPSS
                     FQLKDAFTFLVLVLVLIFRPGGLLGSSEAEKV"
     gene            4366..6159
                     /locus_tag="VIH70_02510"
     CDS             4366..6159
                     /locus_tag="VIH70_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine/isoleucine/valine transporter permease
                     subunit"
                     /protein_id="HEY8115268.1"
                     /translation="MGGVPTRSIVRAGLLGGVGAIYVALVGLYTKFADLELVGEQITL
                     GRVLIVAPAFVAAYAVTRPRIVAGERHSVELRGGLGVGAVSGLVGGAVFATAIWFADW
                     FGVDRIREVFFAVTQELIDFLTFDKGAPAGLLILIGLCVAGGLVGGFARTAPEAIRRS
                     ATAGVLAVILFGLLQRIIPIVFVELGFEADWLYERGRGLTIFGGVIVFVIAAGANALW
                     NRDRGRTRERIRRGVQQAPAAKVGLFVAILAILSILPLLLGTVISEVLGQVMIYALLG
                     LGLNIVVGYAGLLDLGYVAFFAFGAYTTGVLTGAFLNTTTGAATPALSADLNFFVAVW
                     IVALLAAGVGLLIGAPVLRLRGDYLAIVTLGLGEIVSVITASKWAQPLIGGPQGMRGV
                     TKAEVFGLNFQDNSQHFYYLALAFVLLAAFVSWRLSTSRIGRAWNAMREDEQVADAMG
                     ISTTKFKLLAFALGGAIGSLGGALFAVSIGSLTPASFEFTVSITALAVVILGGLGSIP
                     GVLVGALVLIGLPGLLREFEEYRALIYGSALVAIMILRPQGLIPNVRRSRELEEEEVT
                     QDAWAKVTAGPEPVAAVLPGGGLGVEQEDLE"
     gene            6156..6947
                     /locus_tag="VIH70_02515"
     CDS             6156..6947
                     /locus_tag="VIH70_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8115269.1"
                     /translation="MSAPEHLLELTNVAKHFGGLYALSGLSFHVDPGEIVSVIGPNGA
                     GKSTMFNVVTGLYGPDDGSIRFRGANVVGLAPNQVTRLGIARTFQTVHLFPNMTILEN
                     AMVGQHCRTRSGVVGSVLRLPGTRREEHRIRERARESLGFFGSRLAGYREEQPASALS
                     YANRRRLEMARALATDPALVMLDEPTAGMNPRETLELRDHIVRMRDELGLTVLVIEHD
                     MRVVKGVSDRVVALDYGRKIAEGSYDEVANDPQVIEAYLGTKAVG"
     gene            7082..7789
                     /locus_tag="VIH70_02520"
     CDS             7082..7789
                     /locus_tag="VIH70_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8115270.1"
                     /translation="MLELRDVSTHYGLVAVLRNVNVEIFPGEMVCLLGGNASGKTTTL
                     KTILGYVKPTEGDVVLDGEVVTGLPTSEVVSKGISMVPENRRLFANMTVSENLDLGAY
                     QRRDKAKIAQDREKVLETFPRIRERLKQKAGTLSGGEQQMVAMGRALMADPKVLLMDE
                     PSMGLAPILVEQVFEIIKQIRALGRTVFVVEQNANMALSIADRGYVIQTGQVVLADTA
                     RNLLDNPLMREAYLGEL"
     gene            7843..8997
                     /locus_tag="VIH70_02525"
     CDS             7843..8997
                     /locus_tag="VIH70_02525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8115271.1"
                     /translation="MNRTADAVVVGGGVVGASAAYHLAAAGAGRVLVLERADHLAAGS
                     TGACAGGFRFQFSSALNVRLSQASVPMITGFTQEHGLPLDVVQHGYLFLVRDEELWRD
                     FLAANRLHASLGVEAEVLTPEQALELAPGIGLDGLIGATYGPRDGIADPSGLTQGYAT
                     LARRAGAEIELGVAVEGVRTAGGRVRGVDTRDGAIDAGVVVNAAGPWAGVLAATAGVD
                     LPLEPIPRHVVVTGPFSGAPERRTLVIDALTSFYFHREGAGVLMGMGGTREWASFETA
                     IDESFIAEELLPTAVRVFPPLEDASVEHAWVGLYEMTPDRHPIIGEAPGVAGFYLANG
                     FSGHGFQHAPVVGKLLAELIVQGAATTVDITSLGLERFERGALVREGHVV"
BASE COUNT         1280 a         3020 c         3279 g         1431 t
ORIGIN      
        1 tgtacatggg cgaggaggac acgatccggc aggcgcccaa cgtcgcccgg atgcagctgc
       61 tcggcgccga ggtcgtcacg gtcacctccg gcagccgaac gctcaaggac gcgctcaacg
      121 aggcgctgcg ggattgggcg gcgagcgtgg tctccacgca ctacgtcctc ggctcggtcg
      181 cgggacccca tccgttcccc gcgctcgtcc gcgatctcca acgcgtgatc ggcgacgagg
      241 ccagggtgca gctgggcgag cgcgagggtc gcgaccctga catggtggtc gcctgtgtcg
      301 gcggcgggag caacgcgatc gggatcttcg aggcgttcct ccccgaaccg agcgtagggc
      361 tcgtcggcgt ggaggccggg ggcacgggcg acgggctcgg ggagcactgc cggtcgctca
      421 cgctcggcga gccgggcgtg ctgcacggct cgtactccta cctcctgcag gacgagcagg
      481 ggcaggtggt gccgacgcac tcgatctcgg cggggctgga ctacccgggc gtgggtcccg
      541 agcacgcgtt cctcaaggac gcgggccgcg ccacctatgt ctcggtcacc gacggggagg
      601 cgctcgaggg cttccgcgcg ctggccacgc tcgaggggat cctgcccgcg ctcgagccgg
      661 cgcatgcgat cggctggctc ctgcggcgtc cactgcccga gggctcgctc gtactggtca
      721 acctgtccgg ccggggcgac aaggacctcg agacggtgcg ggccgcgtcg ggaccggtcc
      781 ccggggcgcc gagtggctga tctcgagccc gccctgcgat cgcggctgga ggcgggcggt
      841 cgcgccttcg tcccgtacgt caccgggggg ctcgcgggcg tggacgcgga gctgctgcgg
      901 gggctcgagg aggccggggc cgacgcgatc gaggtgggga tcccgttctc cgaccccgtc
      961 atggacgggc cggtgatcca ggaggcctcg cgtcgcgcgc tcggtgaggg cgcgacgcca
     1021 tccagcgtgc tgggctcgat caaggaggcc gggctcgagg tccccgtcgc cgtcatgaca
     1081 tacgcgaacc ccgtctggca cgcgggccac ggggcgtttc tcgacacgtg cgtcgacgcc
     1141 ggcgtcacgg gcgtgatcgt tcccgacctc cccgtcgacg aggcccgcga ctggatcgcc
     1201 gcgtgccggc gagcctcggt ggccccggtc ttcctggcgg cgcccgggac ctcggacgac
     1261 cgcctgcggg cgatcggtga cgcctcgcgg gggttcgtct actgcgtcgc gacctacggg
     1321 gtcacggggg cgcgggatcg gctggccgag acggccgcag agctcgtggg gcgcctccgc
     1381 ccgctcgcgt ccctcccgct cctggtcggg gtcgggatcg ccgagccgcc gcacgcggcc
     1441 gaggcgtgca cattcgcgga cggcgtcgtg gtggggagcg cgctcgtccg cccgttgctc
     1501 gacggagaca cggcgaccgc catcgggctc gcccgcgcgt tccgggcggc gacgcctccg
     1561 taggcacgtg ttcggccgtg tcccgcgcag tccgcgggtg ctcccgcttc cgtacacctt
     1621 tcacgacggt tggccacgac gtcggccacg acgttggcca cgacgtcggc cacgacgtcg
     1681 gccacgacgt cggcaggacc gtcggcatgg ggcggagtgg cccgccacct gcggaatgtg
     1741 ataggtacca cctcgacgga ggggtgagaa agggggcgaa caccgttcct catacgtatc
     1801 cgcgcacgac cacgttcgtt ggaaacgttc ggaggggggt tggatgaaga accgcaagta
     1861 cttcgcgatg ttggcgctcc tcggggcgct gatgctcgtc gccgccgcat gcggcaacga
     1921 cgagggtgga gagacgggtg gtgggggagg cacgtccgcc accggcggca ccgtcgactg
     1981 cgcatccgac gagttcgggt gcgtggagat cgccgccggc gatccgatcc agctggcgtc
     2041 gttgctttcg atctcggggg acaccgcgtt cctgggaacg gacagcaacc acggcgtcga
     2101 gctcgcgatc gaccacctgg ataacaagtt cgatggcacc ccgggtcagc tcctcgggca
     2161 cgacgtcacc ctccagcagg aggacgacgg ctgttcggcc gagggtggcc aggccggcgc
     2221 gaccgcgctg gcggccaacc cggacatcgt cgccgtgatc gggacctcgt gctcgagcgc
     2281 cgcgctcggc gtcgccgaca cgatcctggg tgacaagggc atcctcctgt tctcgccctc
     2341 gaacacgaac ccgggcctga cttcggagga ggcgcaccag cccttctacg cgcgcacggc
     2401 gcacaacgac aagatccagg gcgcgatcgt cgccgacttc gtctttacga agctcggcat
     2461 cgacaccgcc gcgacgatca acgacgagag cccgtacgcg gatggactgg ccgcggcctt
     2521 ccgtgacaac ttcgaggcgc tcggcgggac gatcaccgat accgagcagg tgaacagcag
     2581 cgacaccgac ctcggtccgg tgttcacgaa catcgcggcg accgaccccg gtgtcgtcta
     2641 cgggccgaac ttcaaccccg tctgcgccct cagctacacg cagggctcgg acatcctggc
     2701 gaaggggacg gtccagatcg gctccgacgg ttgcctggag agctcgttcc tggacacggt
     2761 gggcgctccg atcaaggact acacgtatta cgcgtcctcg ccggatgtgt ccgcgtatca
     2821 gagcggcgcc ttctacaaga acgagtacct ccccgcgtac acggatcagt tcggcaagcc
     2881 gacgtcggtg ttcaacgcac acgcgtacga cgccacgaac gttctgttcg acgcgatcga
     2941 ggccgtggcg gtggacgacg ggagcggcaa cctgacgatc tcgcggactg cgctcaaaga
     3001 cgccgtcctg gcgacgagcg gctaccaggg cctgacgggg acgatcacat gcaccccgct
     3061 gggtgactgc gcgaccgacg tgacgatcgg cgtgttcgaa tacgaccact ggccggtcga
     3121 gggtggcaag gacccgaccg acccggtctt ctcggagacc aagacgctgg ccgacgtcat
     3181 ctagttcgac cggcacgaac gctgtgggtg ggccacccgg cccacccaca gcggtgcctg
     3241 gagggatggg ctaggctccc cggcgatggc caccgccacc gacgagcagg tccgcggcgc
     3301 ggaactcgga ctgctcgaac gcctcccctg gcgcaggatc atcctggtgg tgctctcggt
     3361 cgtgatcctg gccgtagtcg cgatcggcgc gtacaagacg ctgaccctgc ccgccgacga
     3421 gcgctatacc gcgacgacct ggaagaactt catcgtcctg ggtctcgccc agggggccat
     3481 catcgggctg atcgcgctcg gctacacgct cgtgtacggg atccttcgga tgatcaactt
     3541 cgcgcatggc gaggtgttca tgttcggagc gttcggggcg ttcttcttct ccgatgcctg
     3601 ggagcagagc gggttcctca acaccaaccc ctggctctcg atggcgctcc tgctgatcat
     3661 cgcgatgctc acgtcgatga gcgtcgccgt cctgctggag cgcatcgcct accgcccgct
     3721 ccgcaacgcc ccccggctgg tgccgctgat cacggcgatc ggggcgtcgc tgttcctgca
     3781 gaacaccgct cgaggcttct tcggggcgca gagccacggg ttcggcgtgc cggaggtgct
     3841 cgacggcacg tggacgatcc tcggtgtcga ggtcgaccgg atccaggtcg tcgtcctcgt
     3901 caccgtgatc gtggcggtca ccgtcctgtc cctgttcgtc tcgaagacca agaccggcaa
     3961 gtcgatgcgc gcggtggccg cggacagcga gatcgcgagc ctgatgggga tccgggtcga
     4021 ccgcgtgatc gtgacgacgt tcgcgatcgg gggcctgctg gcggggatcg cggccgtgct
     4081 gttctcgatc acgttcgagt cggtgagctt cacgatgggg gtccggcccg ggatcgcggc
     4141 gttcaccgcc gcggttctgg gggggatcgg gagcatctcg ggcgccgcgc tcggcggcgt
     4201 cctgctgggg ctcctgcagt cggtgggtcc ggcgctgatc ctcaccggct acggcatccc
     4261 gtcgtcgttc cagctgaagg acgcgttcac gttcctcgtg cttgtcctgg tcctcatctt
     4321 ccggccgggc ggactgctcg gatcgagcga ggcggagaag gtgtgatggg cggggtcccg
     4381 acgcgctcga tcgtccgcgc ggggctgctc ggcggcgtcg gtgcgatcta cgtcgccctg
     4441 gtcgggctct acaccaagtt cgccgacctc gagctcgttg gggagcagat cacgctcggc
     4501 agggtcctga tcgtcgcgcc cgcgttcgtc gccgcgtacg ccgtgacccg cccacggatc
     4561 gtcgcgggcg aacgccactc ggtcgagctg aggggtggcc tcggggtcgg tgcggtgtcc
     4621 gggctcgtcg gcggggcggt cttcgcgacg gcgatctggt tcgccgactg gttcggcgtg
     4681 gaccggatcc gcgaggtctt cttcgccgtc acccaagagc tgatcgattt cctgacgttc
     4741 gacaagggcg cgccggccgg cctgttgatc ctgatcggcc tgtgcgtcgc gggcggcctc
     4801 gtgggggggt tcgcgcgcac cgcaccggag gcgatccgcc gttccgcgac cgccggggtc
     4861 ctcgcggtga tcctcttcgg cctcctccag cggatcatcc cgatcgtctt cgtcgagctc
     4921 gggttcgagg ccgactggct gtacgagcgc gggcgcggcc tcacgatctt cggcggggtc
     4981 atcgtgttcg tgatcgcggc cggcgccaac gcgctgtgga accgcgaccg gggccgaacg
     5041 cgcgaacgga tccgacgcgg tgtccagcag gcgccggccg ccaaggtcgg tctgttcgtc
     5101 gcgatcctgg cgatcctgtc gatcctgccc ttgctcctcg gcaccgtgat ctccgaggtc
     5161 ctcggacagg tcatgatcta cgcgctcctc ggactgggtc tgaacatcgt ggtggggtac
     5221 gcagggctgc tcgacctcgg gtacgtggcc ttcttcgcgt tcggcgccta cacgaccggc
     5281 gtgctcaccg gggccttcct gaacaccacg accggcgccg ccacacccgc gctctccgcc
     5341 gacctgaact tcttcgtggc cgtatggatc gtcgcgctgc tcgcggcggg ggtcggcctg
     5401 ctgatcggcg ccccggtgct ccggctgcgc ggcgactacc tcgcgatcgt gacgctcggg
     5461 ctcggcgaga tcgtgagcgt catcacggcg tccaagtggg cccagccgtt gatcgggggg
     5521 cctcagggga tgcggggcgt caccaaggcc gaggtcttcg ggctgaactt ccaggacaac
     5581 tcgcagcact tctactacct cgcgctcgcc ttcgtcctac tcgccgcgtt cgtctcgtgg
     5641 cgattgtcga cgtcccggat cggccgggcg tggaacgcaa tgcgcgagga cgagcaggtc
     5701 gcggacgcga tggggatcag cacgacgaag ttcaagctgc tggccttcgc gctcggcggc
     5761 gcgatcggat cgctgggcgg cgcgctgttc gcggtcagca tcgggtcgct cacgccggcg
     5821 agcttcgagt tcacggtgtc gatcacggcc ctcgccgtcg tcatcctggg cggcctcggc
     5881 agcatccccg gcgtcctggt cggggcgctc gtgctgatcg ggttgccggg gctcctgcgt
     5941 gagttcgagg agtatcgggc tctgatctat gggagtgccc tggtcgcgat catgatcctg
     6001 cggccgcagg gcctgatccc gaacgtccgg cggagccggg agctcgagga ggaagaggtc
     6061 acccaggacg cctgggcgaa ggtcacggcc ggtcccgagc ccgtcgcggc cgtgttgccg
     6121 ggcgggggcc tcggcgtgga gcaggaggat ctggagtgag cgcgcccgag cacctgctcg
     6181 agctcacgaa cgtcgccaag cacttcgggg gcctgtatgc gctatcgggg ctttcgttcc
     6241 acgtcgaccc cggcgagatc gtctcggtca tcgggccgaa cggggccggc aagagcacga
     6301 tgttcaacgt cgtgaccggc ctgtacggcc ccgacgacgg cagcatccgc ttccgcggcg
     6361 ccaacgtcgt cgggctcgcg ccgaaccagg tcaccaggct cggcatcgcc cgcaccttcc
     6421 agacggtgca cctgttcccg aacatgacga tcctggagaa cgcgatggta ggccaacact
     6481 gccgcaccag gtccggcgtg gtcggctcgg tgctccgtct gcccggcacc cggcgcgagg
     6541 agcatcggat ccgcgaacga gcgcgcgaga gcctcgggtt cttcggctcg cgcctggccg
     6601 gctaccggga ggagcagccg gcatccgcgc tctcgtacgc gaaccggcga cggctcgaga
     6661 tggcgagggc gctggccacg gacccggcgc tcgtgatgct ggacgaaccc acggcgggca
     6721 tgaacccacg cgagacgctg gagctccgcg accacatcgt gcggatgcgc gacgagctcg
     6781 ggctcacggt cctggtgatc gagcacgaca tgcgggtggt caagggagtc tccgaccggg
     6841 tcgtggcgct ggactacggg cgcaagatcg cggaggggtc ctacgacgag gtcgcgaacg
     6901 acccccaggt gatcgaggcg tacctgggga ccaaggccgt cgggtgagcg acgaggcgcc
     6961 aacccccggg atgcggactg ccgatccggc cgaaaccggc gtcgaggtcc agccggtcga
     7021 caccggcgcg ctcccgaccc acgacaaacc tacccgggaa caggacggcg agcggcgtcc
     7081 cgtgctggag ctacgcgacg tgagtaccca ctacgggctg gtcgccgtgc tccgcaacgt
     7141 gaacgtcgag atctttcccg gcgagatggt gtgcctcctg ggcggtaacg ccagcgggaa
     7201 gaccacgact ctgaagacga tcctcgggta cgtcaagcct accgagggcg acgtggtgct
     7261 cgacggcgag gtggtgaccg ggctgccgac gtcggaggtc gtctccaagg ggatctcgat
     7321 ggtcccggag aaccgccggc tcttcgcgaa catgacggtc tcggagaacc tggatctcgg
     7381 cgcctatcag cgtagggaca aggcgaagat cgcgcaggat cgggagaagg tgctcgagac
     7441 gttcccgcgg atccgcgagc gcctcaagca gaaggccggg acgctctccg gcggcgagca
     7501 acagatggtc gcgatgggcc gggcgctcat ggccgatccg aaggtcctgc tgatggacga
     7561 gccttcgatg ggtctcgcgc ccatcttggt cgagcaggtc ttcgagatca tcaagcagat
     7621 ccgcgcactc ggccgcaccg tgttcgtggt ggagcagaac gcgaacatgg cgctctcgat
     7681 cgccgatcgt ggctacgtga tccagacggg gcaggtggtg ctcgcggaca cggcgaggaa
     7741 cctgctggac aacccgctga tgcgagaggc gtacctcggg gagctctgag tgatcgctcc
     7801 ctgggacgcc acggtgtcct cgcgccagac ggggagcccg gtgtgaaccg aaccgccgac
     7861 gccgtcgtgg tggggggagg ggtcgtcggc gcgagcgccg cgtaccactt ggccgcggcg
     7921 ggcgccggac gggtcctggt gctcgagcgc gccgatcacc tggcggcggg gtcgaccggg
     7981 gcatgcgccg gggggttccg cttccagttc tcgagcgcgc tcaacgttcg cctgtcccag
     8041 gcaagcgtgc cgatgatcac ggggttcacg caggaacacg ggctcccgct cgacgtcgta
     8101 cagcacgggt atctgttcct ggtccgcgac gaggagctgt ggcgcgactt cctggccgcc
     8161 aaccggctgc acgcgtcgct gggcgtcgag gccgaggttc tgacgcccga gcaggcgctc
     8221 gagctcgcgc ccgggatcgg gctcgacggg ctgatcggcg cgacgtacgg gccgagggac
     8281 ggcatcgcgg acccgtcggg gctcacgcag gggtacgcga ccctcgcgcg acgcgcgggc
     8341 gccgagatcg agctcggcgt cgccgttgag ggcgttcgga ccgccggggg ccgggtgcgc
     8401 ggcgtcgaca cgcgggacgg cgcgatcgat gcgggcgtcg tcgtgaacgc ggccggtccg
     8461 tgggcggggg tcctggcggc tacggccggc gtggacctgc cgctggagcc gatcccgcgc
     8521 cacgtggtgg tcacggggcc gttctcgggc gcgcccgaac gcaggacgct ggtgatcgac
     8581 gcactcacga gcttctactt ccatcgcgag ggcgccgggg tgctgatggg catgggcggc
     8641 acgcgggagt gggcgtcgtt cgagaccgcg atcgacgagt ccttcatcgc cgaggagctc
     8701 ctgccaaccg cggtccgggt cttcccgccg ctcgaggacg cgtcggtgga gcacgcgtgg
     8761 gttgggctgt acgagatgac gcccgaccgt cacccgatca tcggcgaggc gccgggggtc
     8821 gcggggttct acctcgcgaa cggcttcagc ggccacggct tccagcacgc cccggtcgtc
     8881 ggcaagctgc tcgcggagct gatcgtgcag ggagcggcca cgacggtcga catcacgagc
     8941 ctcggcctcg agcggttcga acgcggcgct ctcgtccggg agggccacgt cgtctgagtg
     9001 ccgggagagg
//
LOCUS       DATBRK010000083        12669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2033478, whole genome shotgun sequence.
ACCESSION   DATBRK010000083 DATBRK010000000
VERSION     DATBRK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033478"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VIH70_02530"
     CDS             <1..1187
                     /locus_tag="VIH70_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY8115272.1"
                     /translation="YNRVSNGMLWFAYHYLWDTVTSPSWGSDVEQAWDAYVDVNRRFA
                     TALAEEGDALGGDTAFLIQDYHLSLVPRILRELVPNALIAHFSHTSIAGPTYFRMLPA
                     RIHDQVLGGLMGADVLGFHSPAWAENFLLSARQFPGARVDLARSRIVTEGREVTVRIH
                     PISVDGRAMREEAATPRVRSLRHALNRWRDNNRLILRVDRLELTKNVVRGFQAYELFL
                     RRNPSWIGRVRFLALLSPSRMELPEYREYTEACLAEARRVNDALGRDDWLPIELRLKD
                     DYIGAIAAYGLYDVLFVNPVIDGMNLVAMEGPLLNRRRGVLVLSRNAGAYGRLGRYAL
                     PVNPFDVDEMADALERAITMPADERTRRSRGLSRLVLANPPARWVGGQLEDLERAKRR
                     RR"
     gene            complement(1184..1972)
                     /gene="otsB"
                     /locus_tag="VIH70_02535"
     CDS             complement(1184..1972)
                     /gene="otsB"
                     /locus_tag="VIH70_02535"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY8115273.1"
                     /translation="MLRRRSRSSWGTTTPPSRQADLDALRQRADVAGVVLDFDGTLAQ
                     IVARPELARPVDGAREALAGLVERYRTVAIVTGRRSDEVAALVDLPHLAYVGLYGLED
                     GAPELVTAVVPRAEAAAAEVPEAWVEDKGVSVAVHYRQAPDPPSARAVLVVALQPVAT
                     GAGLELIEGKMVVELVPPDRPLKGGAVERLVGEHALEALLYAGDDHADLDAFAALDRL
                     SASKDVGVAVRVAVRGPETPLAMVEAADVVVEGPEGLVDLLRTL"
     gene            complement(1911..2501)
                     /locus_tag="VIH70_02540"
     CDS             complement(1911..2501)
                     /locus_tag="VIH70_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEY8115274.1"
                     /translation="MHQPPTNRSFYLSLAASTLRFAIIVALVIGGIVVIDQAFPALSS
                     GDGGGGGDGGGATVPLTGGTIGATGTDTTGPTGETATGGTRPEPSPTVVGVRIAVFNG
                     AGVSGLAGDTQARLEDLGYIATQDPADAPTSYPVTTIYYNAPKDKVEADYLASSFFKK
                     LDGVEVVRLEPDTADIDPSTQVAIFLGNDYATLKAG"
     gene            complement(2518..2796)
                     /locus_tag="VIH70_02545"
     CDS             complement(2518..2796)
                     /locus_tag="VIH70_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEY8115275.1"
                     /translation="MGAQPRDLALELDERTRAVLDFEASWWLEGGPKERLIRERFGIS
                     STRYQQLLMRTIDLPEALRYDPMLVRRLRRLRESRRRKRLARRLGLPR"
     gene            complement(2855..7657)
                     /locus_tag="VIH70_02550"
     CDS             complement(2855..7657)
                     /locus_tag="VIH70_02550"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEY8115276.1"
                     /translation="MSTTDEVTAGALPASVLELLDASVPPERAEALRAFAVAFTRRLP
                     PQDLSERPAAELVGVIIGAFALADTRPEDEFAVRVFEPDASEDGYEALGTVVEVSAAD
                     CPFLYDSVTEELGAWGLSVRRVIHPVIGVERRPDGRIERILHVRKTEDRESVMHFELE
                     QRLTEQERTELEQAIRRVLGDVRLAVRDFKAMKDAARHMIEIARAGSALYPPDEVAET
                     VAFLEWLLDLNFVFLGYREYELVDLPEGRALGAVPGSGLGILSKLAWSEYEKPVPLES
                     IEPNLRARIEGGDLLIYSKTNRPSTVHRRARMDYIGVRKVSAQGRIVGETRMVGLFTS
                     KAYSEPASKTPLLHRKLEQILDAEDLYEGSHDYKAIVTIFESFPKDELFSAPTDELRE
                     QVMGLLQVHETKEVRLFARRDPYGRSVSLLVAMPRDAFDADVRLKLQDLFLQRFDGTT
                     IDDHVALTESGIAQLHFTVHVGHGRIPDVLLDELEREVIEIARTWDDRLLDRLIQEHG
                     EARARTLFERWADRFPDYYKASTDVAVAGLDVARFDELERGEEGFLIGLANQTEEGRT
                     LTHVRLYKVEGRVELSDFVPTLESLGLRVIEEWPTTLLGEDGDERFLHDFGVLDTDHR
                     PIDVETTGTRVAECIGAVWRGECESDSLNRLVVSAGIDWRQVQILRAYRKYHHRVNST
                     FPVEYKNDAFAAHPFVAEQLVRLFEMRFDPSRPRDPEAVEALRMQIRSDLDAVRSLEQ
                     DRILRNALGVIDATVRTNVYVPRRRSIAFKLRSAAVPEMPKPTPLFEIFVYSPSMEAV
                     HLRGGRVARGGIRWSDRRQDYRTEVLGLLKAQMVKNAVIVPTGAKGGFVLKHEIEDPR
                     ALKEEGTRQYETFVRSMLDLTDNLVEGAVVHPASVVVEDEDDPYLVVAADKGTATFSD
                     IANAISAEYDFWLGDAFASGGSTGYDHKELGITARGAWESIKRHFREIGTDVMNEPFT
                     VVGIGDMSGDVFGNGMLYSDQICLVAAFDHRHVFVDPAPNPAVGFAERRRLFELAGSS
                     WDEYDRSKISEGGGVWPRSAKSIDISPETRLALGIEAEELSPSELISAILRAPVDLLY
                     NGGIGTYVKATTESDADVGDRVNDDLRVDGRDIRCRVVAEGGNLGFTQRGRIEFALAG
                     GRINTDFIDNSAGVDTSDHEVNLKILLGLGVARGELAMDERNALLADVEGDVVRHVLY
                     DNFLQAQILSQEAVTSAHHMEAYEGLMVGLETSDVLDRDLEALPNAEEMAERRRTNRG
                     MTRPELAVLLAYAKQNLTNALLLSSLPDSAYLETDLRGYFPPQVVERFGPLLNEHPLR
                     RELIAMLVANDVVNSQGITFVSRVVTETGADAADVTRAYRIARDVTGAVERWEEIEAL
                     VGELEPALLDDLMEGVDRLVEVTARWFLQHVPGQLGRAIEAYSGPFRAFAAAVGEVAP
                     ARWLQERERAAWALMDRGVPEGIARAHALQPFLVHGPNVADVASATGRSLEDVTLAFF
                     LVGDATYLDWLGSRLSEVSTTTRWHRWALQALEEDLRLTRRQVAEKVLGEGDDVRAPV
                     EQALAAFLARRDESMTRLGRFMRGIALEEASDLAALTVAVRQVRALSA"
     gene            7824..9935
                     /locus_tag="VIH70_02555"
     CDS             7824..9935
                     /locus_tag="VIH70_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8115277.1"
                     /translation="MAQLRMLHALATRLNALDDVAAIGEAITTELKTLIDYHNCRIYL
                     LLEDGLTLWPVTFRGELTEYESETPEELVTQVGEGITGYAAITGQTCYAPDALNDERG
                     IQIEGTDEIVESLLAVPLKAADKVTGVIVLSKLGIDQFEEDDQRVLEVLASHASVAYE
                     NASLLQKEREAAKTASALLGLSQALSGAGNVSGVLARVVAAVPSIIDGSVVVAYRRDQ
                     KTGDFIPAHHRGLSPDRPPTIPAEVAVRFLVSFEEPFVIERDMAEQVPTEFWLVDEPT
                     AALIAPMSWEPDEFGALVVLAPDVESTFSERDRDLARAIAQTASLALGSARHVRELER
                     FHELAETIDAIFWEAEPTTLAFTFLSRRASLVLGHETDTSEALPQRWGDHIVPDDRTR
                     MTSTLRAAVATPGDDLDLEYRTRGREGETLWLRDIVHIAVDARGRPVVRGLIVDVTEG
                     KRAEQALRRSEQKYSEAFHREREATRRLRALDEMKNTFLEAVSHDLRTPLTSILGSAV
                     TLEQSGMDIPREDAVDLLRRIASNARKLERLLGDLLDLDRLQRGIITPQRRRIDVGAL
                     VLGAVREFEQLGGRKVECGSDELIANVDPPKVERIVENLLSNASRHTSPDSRIWARVE
                     RREDGLLLVVEDEGGGVPDDLKDAIFEPFRQGPGPASASPGVGVGLSLVARFAELHGG
                     RAWVEDRPGGGASFRILLAEA"
     gene            complement(9940..10890)
                     /locus_tag="VIH70_02560"
     CDS             complement(9940..10890)
                     /locus_tag="VIH70_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016559274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115278.1"
                     /translation="MQARDRNRVASTLAVLILVGSAITPSAAVAGAIPGSGCRVFPAS
                     NIWNTRIDALPVHPLSDTWLRSAQAGSTDLHPDFGPPSYGLPFDVVGRRHAKERVRFT
                     YADESDPGPYPFDARTPIEGGSDRHVLIVERGTCRLYELFGASWNHGDPRAGSGAIFD
                     LDSNRLRPDTWTSADAAGLPILPGLVRWDEVKAGSITHAIRFTLSCTTDAYIWPARHE
                     AGVHDPDCPPMGARFRLKATFDLGGFSPRARTVLRAMQHYGLILADNGSNWYFQGTRD
                     AHWRNHLLDQLKTVPASAFEAVDVSGCMVNADSGRADCPA"
     gene            complement(10949..11020)
                     /locus_tag="VIH70_02565"
     tRNA            complement(10949..11020)
                     /locus_tag="VIH70_02565"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10986..10988),aa:Thr,seq:tgt)
     gene            11051..12034
                     /locus_tag="VIH70_02570"
     CDS             11051..12034
                     /locus_tag="VIH70_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY8115279.1"
                     /translation="MTHTAPTEDILTADIHLEEFMDASVGIAGSVGDIEVLAASFTRY
                     LRAANRSPKTIRTYAEAVDGLARFLTDHGMPRQVSAIRREHVEAFVEDILARWTAATA
                     LNRYQGLRQFFRFLTEDGEITESPMDRMTPPKVPEAPPPVLTDDELRALLKSAEGGSF
                     EQRRDTALLRVMIDTGARVSEIANLAMEDIDLNAGTITVVGKGRRVRTLPLGVKSVRQ
                     LDRYIRARAAYPQVVEPWLWLGTRGRLTDSGIRQLLERRGADAGIARKVTPHMFRHAF
                     AHRWLSDGGNEGDLMRLTGWRTRQMVSRYAASAADERAMAAHRRMGPGDRL"
BASE COUNT         1972 a         4462 c         4342 g         1893 t
ORIGIN      
        1 actacaaccg cgtctcgaac ggcatgttgt ggttcgcgta ccactatctg tgggacacgg
       61 tcacgtcgcc atcgtgggga tcggacgtcg agcaggcgtg ggacgcgtac gtcgacgtca
      121 atcgacggtt cgcgacggcc ctggccgagg agggcgatgc gctcgggggc gacaccgcgt
      181 tcctgatcca ggactaccac ctgtccctcg tccccaggat cctgcgcgag ctggtcccga
      241 acgcgctgat cgcgcatttc tcgcacacct cgatcgcggg tcccacgtac ttccgcatgc
      301 tgccggcgcg aatccacgat caggttctcg ggggcctgat gggggcggac gtcctcgggt
      361 tccactcccc ggcgtgggcc gagaacttcc tgctctccgc ccgtcagttc cccggtgccc
      421 gggtcgacct cgcccgctcg cgcatcgtga ccgagggtcg cgaggtgacg gttcgcatcc
      481 acccgatctc cgtggacggc cgcgccatgc gcgaggaggc ggcgacgccg cgggtccgca
      541 gcctccggca cgcgctcaac aggtggcgcg acaacaaccg cctcatcctg cgcgtggacc
      601 ggctcgagct caccaagaac gtggtccgcg ggttccaggc gtacgagctc ttccttcgcc
      661 gcaacccgtc gtggatcggt cgggtccggt tcctcgcgct tctctcgccc tcgcggatgg
      721 agttgcccga gtaccgggag tacacggagg cctgcctggc ggaggcccgc cgcgtgaacg
      781 acgccctcgg ccgcgacgac tggctgccga tcgaactccg cctgaaggac gactacatcg
      841 gcgcgatcgc cgcgtacggc ctgtacgacg tgctgttcgt gaaccccgtg atcgacggga
      901 tgaaccttgt cgcgatggag ggaccgctgc tgaaccggcg acgcggcgtg ttggtgctct
      961 cccgcaacgc gggggcgtac gggcggctcg gcaggtacgc gctgcccgtg aacccgttcg
     1021 acgtggacga gatggccgac gcgctcgagc gcgcgatcac gatgcccgcg gacgagcgca
     1081 cccggcgctc ccgggggctc tcccggctgg tgctcgcgaa cccgccggcc cggtgggttg
     1141 ggggacagct cgaggatctc gagcgcgcca agcggcggcg gcgctagagc gtccgcagga
     1201 ggtcgacgag gccctccggc ccctcgacca ccacgtcggc cgcctcgacc atcgcgagcg
     1261 gcgtctccgg tccacggacg gcgacgcgca cggccacgcc gacgtccttc gaggccgaca
     1321 gccggtcgag ggcggcgaag gcatcgagat ccgcgtgatc gtcgcccgcg tacaggagcg
     1381 cctcgagcgc gtgctctccg acgaggcgct cgaccgcgcc gcccttaagc ggccggtccg
     1441 gcgggacgag ctcgaccacc atcttgccct cgatgagctc cagacctgcc ccggttgcaa
     1501 ccggctgcag cgcaaccacc agcacggctc tggccgacgg cggatccggc gcctgtcggt
     1561 aatggaccgc gaccgaaacc cccttgtcct cgacccaggc ctccgggacc tcggcggcgg
     1621 ccgcctccgc acgcggcacg accgcggtca ccagctctgg cgcgccgtcc tccagcccat
     1681 acaggccgac gtacgcaaga tgcgggaggt cgaccagcgc tgcgacctcg tccgaacgcc
     1741 gcccggtgac gatcgcgacg gtgcgatacc gctcgacgag cccggcgagc gcctctcggg
     1801 cgccgtcgac cggccgtgcg agctcggggc gggccacgat ctgggcgagc gtgccgtcga
     1861 agtccaggac gacgcccgcg acgtccgccc gctgccgcag cgcgtcgagg tcagcctgcc
     1921 ttgagggtgg cgtagtcgtt ccccaggaag atcgcgacct gcgtcgaagg atcgatgtcg
     1981 gccgtgtcgg gctcgagcct gaccacctcg accccgtcga gcttcttgaa gaagctgctg
     2041 gcgaggtagt ccgcctcgac cttgtccttg ggggcgttgt agtagatcgt cgtgaccggg
     2101 tacgaggtgg gcgcgtcggc cgggtcctgg gtcgcgatgt acccgaggtc ctccaaccgg
     2161 gcctgcgtgt cgccggccag gccgctgacc cccgcgccgt tgaacaccgc tatccggacg
     2221 ccgacgacgg tcggactcgg ctctgggcgc gttccgccgg tggccgtctc gccggtcggc
     2281 cccgtggtgt cggtgccggt ggcgccgatc gtgccgcccg tcaacggaac ggtcgcgcca
     2341 ccaccatccc cgccgccgcc gccgtcgccg gaggacaacg cggggaaggc ctgatcgatc
     2401 acgacgatac ccccgatgac cagggcgacg atgatcgcga aacggagcgt gctggcggcc
     2461 aaggagaggt agaagctcct gttggtcggt ggctggtgca cgccatccta cccccggcta
     2521 ccggggaagc ccaagccgcc gcgcgaggcg cttgcgtcga cgcgactcgc gcagcctgcg
     2581 caaccgacgc accagcatcg ggtcgtatcg cagcgcctcc gggagatcga tagttcgcat
     2641 caggagctgc tgataccgcg tcgacgagat gccgaaacgc tcgcggatca ggcgctcctt
     2701 cgggccccct tccagccacc acgaggcctc gaagtccagg accgcgcggg tgcgttcgtc
     2761 gagctcgagc gcgagatcgc gcggttgggc tcccacggag ggcagggtag cgggcgccgc
     2821 cgacgctgca agcacccggc ggcgactcga cggctcaggc cgagagcgcc cggacctgcc
     2881 gcaccgccac cgtgagggcg gcgagatcgc tggcctcctc gagcgcgatc ccgcgcatga
     2941 accggcccag ccgggtcatc gactcatccc gccgcgcgag gaacgcggcg agggcctgct
     3001 cgacgggcgc ccgcacgtca tcaccctccc ccaggacctt ctcggccacc tgccggcggg
     3061 tcagccggag gtcctcctcc agcgcctgga gcgcccagcg gtgccaccgc gtggtcgtcg
     3121 agacctcgga caggcgcgaa cccaaccagt cgaggtaggt cgcgtctccc acgaggaaga
     3181 acgcgagggt cacgtcctcg agtgaccggc cggtagccga cgccacgtcc gccacgttcg
     3241 gaccgtgcac gaggaagggc tgcagggcgt gcgcacgcgc gatcccctcg gggacgcccc
     3301 ggtccatcag cgcccacgcc gcgcgttcgc gctcctggag ccatcgggcc ggcgcgacct
     3361 ccccgacggc cgcggcgaac gctcggaacg gccccgagta cgcctcgatc gcccggccga
     3421 gctgtcccgg gacgtgctgc aggaaccacc gcgccgtcac ctcgaccagc ctgtcgacgc
     3481 cctccatcag gtcgtccagg agcgcgggtt cgagctcgcc gacgagcgcc tcgatctcct
     3541 cccaccgttc gacggcgccc gtgacgtcgc gcgcgatccg gtacgcgcgc gtgacgtccg
     3601 cggcgtccgc cccggtctcg gtcacgacgc gcgagacgaa ggtgatgccc tgcgagttca
     3661 cgacgtcgtt ggcgaccagc atcgcgatca gctcgcgacg cagcgggtgt tcgttcaaca
     3721 gcgggccgaa ccgctcgacc acctgcggcg ggaagtaccc gcgcaggtcc gtctcgaggt
     3781 acgccgagtc cgggagcgag gacaacagga gcgcgttcgt gaggttctgc ttggcgtacg
     3841 cgaggagcac cgccagctcg gggcgcgtca tgccgcggtt cgtgcgccgg cgttcggcca
     3901 tctcctccgc attcgggagg gcctcgaggt cacggtcgag cacgtcgctc gtctcgagcc
     3961 cgaccatcag gccctcgtac gcctccatgt ggtgggcgga cgtgaccgct tcctgcgaga
     4021 ggatctgcgc ctgcaggaag ttgtcgtaca gcacgtggcg gacgacgtct ccctcgacgt
     4081 cggcgagaag ggcgttgcgc tcgtccatcg cgagctcgcc acgcgccacc ccgagcccga
     4141 gcaagatctt gaggttaacc tcgtggtccg aggtatccac tcccgccgag ttgtcgatga
     4201 agtcggtgtt gatcctcccg cctgcgagcg cgaactcgat ccggccgcgc tgcgtgaagc
     4261 cgaggttgcc gccctcggcc accacgcggc accggatgtc cctgccgtcc acgcggagat
     4321 cgtcgttcac gcggtcgccg acgtcggcgt ccgactcggt cgtcgccttg acgtacgtcc
     4381 cgatgccgcc gttgtagagc aggtcgacgg gggcgcgcag gatcgccgag atcagctcgc
     4441 tcggcgagag ctcctcggcc tcgatgccga gcgcgaggcg ggtctcgggc gagatgtcga
     4501 tcgacttggc cgagcgcggc cacacgccgc cgccctccga gatcttggag cggtcgtact
     4561 cgtcccagct cgacccggcc agctcgaaca accggcggcg ttcggcgaac cccacggccg
     4621 ggttcggcgc cgggtcgacg aacacgtgtc gatggtcgaa cgccgcgacc aggcagatct
     4681 gatccgagta cagcatgccg ttgccgaaca cgtcgcccga catgtcgccg atgccgacca
     4741 cggtgaacgg ctcgttcatc acgtcggtgc cgatctcccg gaagtgccgc ttgatcgact
     4801 cccaggcgcc gcgcgccgtg atgccgagct ccttgtggtc gtatccggtc gacccacccg
     4861 aggcgaacgc gtcacccagc cagaagtcgt actcggcaga gatcgcgttc gcgatatccg
     4921 agaacgtcgc ggtccccttg tcggccgcga ccacgaggta cggatcgtcc tcgtcctcca
     4981 ccacgacgga cgccggatgg acgaccgcac cctcgacgag gttgtccgtg aggtcgagca
     5041 tgctccgcac gaacgtctcg tactggcggg tcccctcctc cttgagggcc ctcggatcct
     5101 ctatctcgtg cttgaggacg aagcccccct tcgcacccgt cggcacgatg acggcgttct
     5161 tgaccatctg cgccttgagg agcccgagga cctcggttcg gtagtcctga cgccggtcgg
     5221 accagcggat cccgccgcgg gccacccgcc cgccgcggag gtggaccgcc tccatcgagg
     5281 gggagtagac gaagatctcg aagagcgggg tcggcttggg catctcgggg acggcggcgg
     5341 accgcagctt gaacgcgatc gagcggcgcc tcgggacgta gacgttcgtt cggaccgtcg
     5401 cgtcgatgac cccgagcgcg ttgcgcagga tccggtcctg ctcgagggag cgcacggcgt
     5461 caaggtccga acggatctgc atgcggagag cttcgacggc ctcggggtcg cgaggtcgcg
     5521 acgggtcgaa ccgcatctcg aacagccgaa ccagctgctc ggcgacgaag gggtgcgccg
     5581 cgaaggcgtc gttcttgtac tcgaccggga aggtcgagtt cacgcggtga tggtacttgc
     5641 gataggcgcg caggatctgc acctgccgcc aatcgatccc ggccgacacg accaggcgat
     5701 tgagcgaatc ggattcgcac tccccgcgcc acaccgcgcc gatgcactcg gcgacgcgcg
     5761 tccccgtcgt ctcgacgtcg atcggccggt ggtcggtgtc gagcacgccg aagtcgtgca
     5821 ggaagcgctc gtcgccgtcc tcgccgagca gcgtggtcgg ccactcctcg atcacgcgca
     5881 gaccgagcga ctcgagggtc ggcacgaagt ccgagagctc gacccttccc tccaccttgt
     5941 acaggcggac gtgcgtgagg gtccgcccct cctcggtctg gttggccaga ccgatcagga
     6001 acccctcctc cccccgctcc agctcgtcga agcgcgccac gtcgaggccg gcgaccgcga
     6061 cgtcggtcga ggccttgtag tagtcgggga accgatccgc ccaccgctcg aacagcgtgc
     6121 gggcgcgcgc ctcgccgtgc tcctggatca gacggtcgag cagccggtcg tcccaggtgc
     6181 gcgcgatctc gatgacctcg cgctcgagct cgtccaggag cacgtccggg atccggccgt
     6241 gaccgacgtg cacggtgaag tggagctgcg cgatcccgct ctcggtgagc gcgacgtggt
     6301 cgtcgatcgt ggtcccgtcg aaccgctgga ggaacagatc ctgcagcttg agccggacgt
     6361 ccgcgtcgaa ggcgtcgcgg ggcatcgcca ccagcagcga cacgctccgt ccgtacgggt
     6421 cgcggcgcgc gaacaggcgg acctccttgg tctcgtggac ctgcaggagc cccatcacct
     6481 gctcgcgcag ctcgtcggtc ggggccgaga acagctcgtc cttggggaag ctctcgaaga
     6541 tcgtgacgat cgccttgtag tcgtgcgaac cctcgtacag gtcttccgcg tcgaggatct
     6601 gctccagctt gcggtgcaac agcggcgtct tgctggccgg ctcggagtac gccttcgagg
     6661 tgaacaggcc gaccatccgc gtctcgccga cgatgcgtcc ctgcgccgag accttccgga
     6721 cgccgatgta gtccatccgc gctcgccgat ggacggtgga cggccggttc gtcttcgagt
     6781 agatcaggag gtcaccgccc tcgatgcgcg cccgcaggtt cggctcgatc gactcgagtg
     6841 gcaccggctt ctcgtactcg gaccaggcga gcttcgacag gatccccagg cccgaaccgg
     6901 gcacggcccc gagcgcgcgt ccctcgggca gatcgacgag ctcgtactcg cgatagccga
     6961 ggaagacgaa gttcaggtcc agcaaccatt cgaggaaggc aaccgtctcg gctacctcgt
     7021 ccggggggta cagcgccgaa ccggcccgcg cgatctcgat catgtggcgc gccgcgtcct
     7081 tcatcgcctt gaagtcgcgg accgcgagcc ggacgtcgcc gaggaccctg cggatcgcct
     7141 gctcgagctc cgtccgttcc tgctcggtga gtcgctgctc cagctcgaag tgcatgacgg
     7201 attcgcggtc ttcggtcttg cggacgtgca ggatccgttc gatccggccg tcgggtcggc
     7261 gctcgacgcc gatcaccggg tggatcaccc gccggacgct caggccccag gccccgagtt
     7321 cctcggtgac cgagtcgtac aggaaggggc agtccgcggc gctgacctcc acgacggtgc
     7381 cgagcgcctc gtagccgtcc tcggacgcgt cgggctcgaa gacacggacg gcgaactcgt
     7441 cctccggccg ggtgtcggcc aacgcgaagg ccccgatgat cacgccgacc agctccgcgg
     7501 ccggtcgttc ggacaggtcc tgcggcggca gccgccgcgt gaaggccacg gcgaacgccc
     7561 ggagggcctc ggcccgttcc ggcggcaccg aggcgtcgag gagctccagc acggaggccg
     7621 gcagagcgcc cgcggtcacc tcgtccgtgg tgctcacgtt gcacatggta cccgtggccg
     7681 aacacgcccc cgacctgcgg cttcaggaag aggccggacc agccgaggtc atgagaggat
     7741 ggccgggccc gtccgggccc tgcgggatgg tcgagaccag caaccgcgtc agcggacccg
     7801 tcgatgaaca cgagttgcgc tccgtggcgc agctgcggat gctgcacgcc ctggcgacgc
     7861 ggctgaacgc gctggacgac gtcgccgcga tcggcgaggc catcaccacc gagctcaaga
     7921 cgctgatcga ctaccacaac tgccggatct acctcctgct cgaggacggc ctcacgctgt
     7981 ggccggtcac ctttcgtgga gagctcacgg agtacgagtc cgagacgccc gaggagctgg
     8041 tcacgcaggt cggcgagggc atcaccggct atgccgcgat cacggggcag acgtgctacg
     8101 cgcccgacgc cctgaacgac gagcggggca tacagatcga aggcaccgac gagatcgtgg
     8161 aatcgctcct ggccgtaccc ctcaaggccg cggacaaggt gaccggcgtc atcgtgctct
     8221 cgaaactggg gatcgatcag ttcgaggagg acgaccaacg ggtgctcgag gtcctcgcct
     8281 cgcacgcatc cgtcgcatac gagaacgcca gcttgctgca gaaggaacgc gaggcggcga
     8341 agacggcgtc ggcactgctc ggtctgtcgc aggcgctcag cggcgccggg aacgtctccg
     8401 gcgtgctcgc gcgcgtcgtc gccgccgtgc cgtcgatcat cgacggttcg gtcgtcgtcg
     8461 cctatcgtcg cgaccagaag accggtgact tcatcccggc ccaccatcgt ggtctctccc
     8521 ccgaccggcc accgacgatc cccgccgagg tcgcggttcg tttcctcgtc tccttcgagg
     8581 agcctttcgt gatcgaacgc gacatggccg agcaggtccc gaccgagttc tggctcgtcg
     8641 acgagccgac ggcggcgctc atcgccccga tgtcgtggga gccggacgag ttcggcgcgc
     8701 tggtggtcct ggcgcccgac gtcgagagca ccttctccga acgcgaccgc gacctggcgc
     8761 gcgcgatcgc ccagaccgcc tcgctcgccc tgggcagcgc acggcacgtg agggagctcg
     8821 agcggttcca cgagctcgcg gagacgatcg acgcgatctt ctgggaggcc gagccgacga
     8881 cgctcgcgtt cacgttcctg tcgagacggg cgtccctggt gctcggacac gagacggaca
     8941 cgtcggaggc gctgccgcag cgatggggcg accacatcgt cccggacgac cgcacgcgga
     9001 tgacctcgac gctccgggcc gccgtcgcca cgcccggcga cgacctcgac ctggagtatc
     9061 ggacccgggg gcgggagggg gagaccctct ggctccgcga catcgtccac atcgccgtcg
     9121 acgccagagg gaggcctgtc gtccgaggcc tcatcgtcga cgtgacggaa ggcaagcgcg
     9181 ccgagcaggc gctgcgccgc agcgagcaga agtactccga ggccttccac cgcgaacgcg
     9241 aggccacccg gcgccttcgg gcgctcgacg agatgaagaa cacgttcctc gaggccgtct
     9301 cgcacgatct ccgcaccccg ctcacgtcga tcctgggctc cgcggtcacc ctcgagcagt
     9361 cgggcatgga catcccccgc gaggacgcgg tcgacctgct ccggcgcatc gcgtcgaacg
     9421 ctcgcaagct cgagcggctg ctcggcgacc tgctcgacct cgaccggctg caacgcggga
     9481 tcatcacgcc gcagcgccgg cggatcgacg tgggcgcgct cgtcctcggc gcggtacggg
     9541 agttcgagca gctcggcggg cgcaaggtcg agtgcgggtc cgacgagctc atcgcgaacg
     9601 tcgacccccc caaggtcgag cggatcgtcg agaacctgct gtccaacgcc tcacggcaca
     9661 cgtcgcccga tagccgcatc tgggcacgag tggagcggcg cgaggacggg ctcctactcg
     9721 tcgtcgagga cgaggggggc ggcgtcccgg acgacctcaa ggacgcgatc ttcgagccct
     9781 tccggcaggg ccccggtccc gcgagcgcgt cgcccggcgt cggcgtcggg ctgtcgctcg
     9841 tcgcccgctt cgccgagctc cacggcgggc gggcgtgggt cgaggaccgt cccggtggtg
     9901 gcgcctcctt caggatcctg ctggccgagg cctgaggctt cacgcgggac agtcggctct
     9961 cccgctatcg gcgttcacca tgcacccgct cacgtcgacg gcctcgaacg cggacgccgg
    10021 cacggtcttg agctgatcga gcagatggtt gcgccagtgg gcgtcacgcg tgccctggaa
    10081 gtaccagttc gagccgttgt cggcgaggat cagcccgtag tgctgcatcg cgcgcaggac
    10141 cgtccgcgcc ctcgggctga acccgccgag gtcgaaggtc gccttgagcc ggaagcgcgc
    10201 gcccatcggc ggacagtcgg ggtcgtggac accggcctcg tggcgggccg gccagatgta
    10261 cgcgtccgtc gtgcacgaga gcgtgaaccg gatcgcgtgc gtgatgctgc ccgccttgac
    10321 ctcgtcccag cgcacgagcc ccggcaggat cgggagcccg gcggcgtcgg cggaggtcca
    10381 ggtgtcgggg cggagccggt tcgagtccag gtcgaagatt gcaccgctcc ccgcgcgcgg
    10441 gtcaccgtgg ttccacgacg cgccgaacag ctcgtagagt cggcacgtgc cgcgctcgac
    10501 gatcagcacg tgccgatccg agccgccctc gatcggcgtt cgcgcgtcga acgggtaggg
    10561 gcccgggtcg ctctcgtcgg cgtaggtgaa tcggacgcgt tccttcgcgt gacggcgccc
    10621 cacgacgtcg aacgggagcc cgtaggacgg cggcccgaag tccggatgca ggtccgtcga
    10681 ccccgcctgg gccgaccgga gccaggtgtc gctcagcggg tggaccggga gcgcgtcgat
    10741 ccgcgtgttc cagatgttcg acgcggggaa gacccggcaa ccagatccgg ggatggctcc
    10801 ggcgacggcg gccgagggcg tgatcgcgga tccgacgagg atgaggacgg cgagcgtact
    10861 cgcgacgcgg ttccggtcac gagcctgcat ggccctggca tcggcactcc ggccccccga
    10921 ccttatcgga cgagatcggg aggtctcgag ccggcggtcg gattcgaacc gacgacctgc
    10981 tgtttacaag acagctgctc tgcccctgag ctacgccggc gtcgatgacc tgcggaaacg
    11041 ctccaagggg ttgacgcaca ccgctcctac agaggatatt ctcacagcgg atatccacct
    11101 ggaggagttc atggacgcgt ccgtaggcat cgcgggaagt gtaggtgaca tcgaggtact
    11161 ggcggcgtcg tttaccaggt acctccgggc cgcgaatcga agtccaaaga ccatccgcac
    11221 ctacgccgag gcggtcgacg gcttggcgcg attcctcacc gatcacggga tgcctcggca
    11281 agtctcagcg attcggcgcg agcacgtcga ggccttcgtc gaggacatcc tcgctcggtg
    11341 gaccgcggcg acggcattga accgctacca ggggctgcga cagttcttcc ggttcctaac
    11401 cgaggatggc gagatcaccg agagtccgat ggaccgcatg acgccgccca aggttcccga
    11461 agctcccccg cccgttctca ccgacgacga gctccgtgcg ctcctgaagt ccgccgaggg
    11521 cggatccttc gagcagcgcc gggacaccgc gcttctgcgg gtcatgatcg acaccggagc
    11581 acgagtgtcg gagatcgcca acctagccat ggaggacatc gacctgaacg ccgggaccat
    11641 caccgtcgtg ggaaagggcc ggcgcgtccg aacgctcccg cttggggtga agtcagttcg
    11701 gcagctggac cgctacatcc gtgcgcgggc ggcctacccg caggtcgtcg aaccttggct
    11761 ctggctgggg acgagaggcc ggcttacaga cagcgggata cgccagctcc tcgagcgccg
    11821 cggcgcggat gctggcatcg cccgaaaggt gaccccgcac atgttccgcc acgcgttcgc
    11881 gcatcgttgg ttgagcgatg gcgggaacga gggagacctg atgagactga cggggtggcg
    11941 cacccgccag atggtgagta ggtacgccgc gtccgcggcc gacgagcgag ccatggctgc
    12001 tcaccggcgg atgggacctg gggaccggct ctaacgagat ggggccgaac gtcgcggcca
    12061 tcacgaaaaa gcccgagcac gagcatgccg cgcccatcgg cgctcgcgat tccccgcgga
    12121 acactgtatg cgatcgcgaa gactaatggg gcagcgaggc ctgagcccgt cgaggcgggc
    12181 ccaccaggca ccgctcgggg atcgtttggc ctccgagcgg attgcggagg cgagtcaaga
    12241 acagtagatg tcctcggcga cggatatggc cgcttcccag ccggacgagt tctgggcgac
    12301 gacgacgacc ttcgagcggg aagcgccacc actactcgaa cgccaactac gtcctcctcg
    12361 ggcagatcgt ggaggaggtg accggccacc cccctagggt acgcgcacgc ggcatatgcc
    12421 ttgcgcgccc tcgcccacga gggttacggc cacgaccgcc gcctcatggt ggtcctagct
    12481 gagaccgcgc ctgctaagtc tagctgagac cgcggctgct aagtcacccg atgacgtcat
    12541 gcgccgattg cacgagcgct gggctgaggt ctaccgggcg ggtcgacgcg tcaagttcgc
    12601 gagcttcccc atcgagttca acacctatgc cacccgcgta cctgacgaac tgcgcgcgga
    12661 ggttgagtc
//
LOCUS       DATBRK010000084         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1309123, whole genome shotgun sequence.
ACCESSION   DATBRK010000084 DATBRK010000000
VERSION     DATBRK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309123"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VIH70_02575"
     CDS             <1..242
                     /locus_tag="VIH70_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115280.1"
                     /translation="AQHPLQGGADMTRRPVGSDGVHRQGDALVAICEQDGVPYLRWET
                     FDDVREALEEADTLLGPLGAERCPGWRTSSRTPST"
     gene            complement(251..337)
                     /locus_tag="VIH70_02580"
     tRNA            complement(251..337)
                     /locus_tag="VIH70_02580"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(301..303),aa:Ser,seq:gga)
     gene            complement(405..494)
                     /locus_tag="VIH70_02585"
     tRNA            complement(405..494)
                     /locus_tag="VIH70_02585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(458..460),aa:Ser,seq:cga)
     gene            583..1011
                     /locus_tag="VIH70_02590"
     CDS             583..1011
                     /locus_tag="VIH70_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8115281.1"
                     /translation="MKAERLRKIPLFAGLSDHQYERLERWTDEIDVPTNKHLVNQGAY
                     PHEFMVIETGTAEVTHDGVHLADLGPGDFFGEMALLLNVPRTATVAATSDVTLVVMHD
                     RNFRAMEEEIPAVAKRVHAIMEERRAKSADQGIETPEAKE"
     gene            complement(1190..1621)
                     /gene="tadA"
                     /locus_tag="VIH70_02595"
     CDS             complement(1190..1621)
                     /gene="tadA"
                     /locus_tag="VIH70_02595"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY8115282.1"
                     /translation="MMRLALEEARAAAAGGDVPVGAVIVRGDEILARAGNAREREQDP
                     TAHAEILALRQASGAIGSWHLEGCAIVVTLEPCAMCAGAIVLARLDRLVFGASDPKAG
                     FAGSLGDLVRDGRLNHEVDVTVGVLAEECGDVLRAFFADRR"
     gene            complement(1646..1999)
                     /locus_tag="VIH70_02600"
     CDS             complement(1646..1999)
                     /locus_tag="VIH70_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY8115283.1"
                     /translation="MTVVDRERFEDLVMEAVDSLPPWVRERLENIELILEDRPPRGEP
                     NLLGRYHGVPLTRRGQFHSGPTFPDTITLYQRTIEGVARDEEHLRQVIAHTVEHEVAH
                     FFGISDDRLREIDRY"
     gene            complement(1996..3024)
                     /gene="speB"
                     /locus_tag="VIH70_02605"
     CDS             complement(1996..3024)
                     /gene="speB"
                     /locus_tag="VIH70_02605"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY8115284.1"
                     /translation="MSDPPDFRLERSELRGEDEYTEFGPDDLEYYVDARGTFDKVPLC
                     PDDESLSASGADVAIVGAPTDDGTSGRPGARFGPRAIRSAPTTWGRDAWSIQLEVEPY
                     EWLTVVDAGDAPVVPSRHARALRVIHEKVVRVASAGAIPVVLGGDHSITYPSAAAVAR
                     HVWPRKVGVVHFDAHADAADSISGSLISHGTPMRRLIDEGWVRGSNFVQVGLRGYWPE
                     KATFDWMREQGFRWHTIVEVEERGVDAVIDDAIAEALEGSEVVYLSVDIDVVDPGMAP
                     GTGTPEPGGMTGRELLRAVRRIVGAVELAGMDVVEVSPPYDQSEVTSMLAYRVVMEAL
                     SSLAARRG"
     gene            complement(3071..>3298)
                     /locus_tag="VIH70_02610"
     CDS             complement(3071..>3298)
                     /locus_tag="VIH70_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115285.1"
                     /translation="AVTAEGPRTIAGMRAECFHATGGSAEAVRRVVWCFSRGGLLLSL
                     FDGVQDGRVTSAEATHISPGVAPSELVTPTT"
BASE COUNT          519 a         1213 c         1115 g          451 t
ORIGIN      
        1 aagcccagca cccgctgcag ggcggggctg atatgacccg tcgacccgtc ggctccgacg
       61 gcgtacatcg ccagggggac gcgctcgtcg cgatctgcga gcaggacggt gtcccgtacc
      121 tgcgatggga gacgttcgac gacgtgcggg aggcgctcga ggaggcggac acgctcctgg
      181 gccccctggg cgccgaacgc tgtcccggat ggaggacctc gtcacgcaca ccaagcacct
      241 gaaccccgag cggtggccgc gggattcgaa cccgcgaggc ggttgcccac ctacacgctc
      301 tccaggcgtg ccccttcgtc cgctcgggca ggccaccgcc gtcgagtcta gcggcggggt
      361 agtgccgaaa cggccgcccc ttccgaggcg gccggctggt ctcgcggagg cgacaggatt
      421 cgaacctgtg agaggcggat taggccccta accgctttcg aggcggtccc gttcgtccgc
      481 tccggcacgc ctccggggcg agtctagcaa gcggcctccc gccggcctgt gcgaggattc
      541 gacgcggatc gagggagcgc cacggagaac ggaggaagcc ccatgaaggc cgagcgtctg
      601 cggaagatcc ccctgttcgc ggggctgagc gaccaccagt acgaacgact cgaacgctgg
      661 accgacgaga tcgacgtccc cacgaacaag cacctggtga accagggggc ctacccgcac
      721 gagttcatgg tgatcgagac cgggacggcg gaggtcacgc acgacggggt gcacctcgcg
      781 gatctggggc ccggcgattt cttcggcgag atggcgctgc tgctcaacgt gccccggacc
      841 gccaccgtcg cggcgacctc cgacgtcacc ctcgtggtca tgcacgatcg gaacttccgc
      901 gcgatggagg aggagatccc cgccgtcgcc aaacgcgtgc acgcgatcat ggaggagcgt
      961 cgggccaagt cggcggacca ggggatcgag acgccggagg cgaaggagta ggcgagggcg
     1021 gtaggggtcc ttcgctcgaa cgccggcgtg agggtcctga cagacagggg tctgacatcg
     1081 cgcccgcatc gggagcgtcg gtgtcagggt tctgacagac aggatctgac ggacgcggcg
     1141 agaaggacca ggggcttcca ggacgactca cgcaggaaga ccaccccttt cagcggcgat
     1201 cggcgaagaa tgcccgcagc acgtcgccgc actcctccgc cagcacgccg accgtgacgt
     1261 cgacctcgtg gttgaggcga ccgtcccgga cgaggtcgcc gagcgatccc gcgaaccccg
     1321 ccttcgggtc gctggcgccg aacaccagcc gatcgagccg tgcaagcacg atcgcgcccg
     1381 cgcacatcgc gcacggttcg agcgtgacga cgatcgcgca cccctcgagg tgccagctcc
     1441 cgatcgcgcc cgacgcctgc cggagcgcca ggatctcggc gtgggcggtg gggtcctgtt
     1501 cgcgttcgcg agcgttgccc gcccgcgcca ggatctcgtc gccccgaacg atcaccgcgc
     1561 ccaccggcac gtcccccccc gctgccgccg cccgcgcctc ctcgagcgcg agccgcatca
     1621 tccgctcgtc gggggcgtcc accggtcagt accggtcgat ctcgcgcagc cggtcgtcgc
     1681 tgatcccgaa gaagtgcgcc acctcgtgct cgaccgtgtg cgcgatcacc tggcgcagat
     1741 gctcctcgtc gcgcgccacc ccctcgatcg tccgctgata cagcgtgatc gtgtcgggga
     1801 aggtgggccc cgaatggaac tggccgcgcc tggtcagcgg gaccccgtgg tagcgcccga
     1861 ggaggttcgg ctcgcccctg ggcggacgat cctccaggat cagctcgatg ttctcgagtc
     1921 gttcgcgcac ccacggcggc aacgagtcga ccgcctccat gacgaggtcc tcgaaccgct
     1981 ctcggtcgac cacggtcacc cgcgccgcgc ggcgagcgac gacagcgcct ccatgaccac
     2041 acgataggcg agcatcgacg tgacctcgct ctggtcgtag ggcggcgaga cctcgaccac
     2101 gtccattccg gccagctcca cggcgccgac gatcctgcgc accgcgcgca gcagctcgcg
     2161 cccggtcatc ccgcccggct ccggcgtccc cgtccccggc gccatccccg ggtcgacgac
     2221 gtcgatgtcg accgagaggt agacgacctc cgaaccctcc aacgcctcgg cgatcgcgtc
     2281 atcgatcacc gcgtccaccc cgcgctcctc gacctcgacg atcgtgtgcc accggaaccc
     2341 ctgctcccgc atccagtcga aggtggcctt ctcgggccag tacccgcgca ggccgacctg
     2401 gacgaagttc gagccccgca cccagccctc gtcgatcagc cggcgcatcg gggtgccgtg
     2461 cgagatgagc gatccggaga tcgagtccgc cgcgtcggcg tgcgcgtcga agtgcaccac
     2521 gccgaccttc ctcggccaca catggcgggc gacggccgcc gccgacgggt acgtgatcga
     2581 gtggtccccg ccgaggacga ccgggatcgc gcccgcactg gcgacccgga ccaccttctc
     2641 gtggatcacc cggagcgccc gggcgtgacg ggagggcacg accggcgcgt cgccggcgtc
     2701 gacgacggtc agccactcgt agggttcgac ctcgagctgg atcgaccacg cgtcccgtcc
     2761 ccacgtggtc ggcgccgacc ggatcgcacg cgggccgaac cgcgcacccg gccgtcccga
     2821 ggtcccatcg tccgtcggcg ccccgacgat cgccacgtcc gcgccgctcg cggaaaggct
     2881 ctcgtcgtcg ggacacagcg gcaccttgtc gaaggtcccg cgggcgtcga cgtagtactc
     2941 caggtcgtcc ggcccgaact cggtgtactc gtcctcgccg cggagctcgc tccgctcgag
     3001 gcggaagtcg ggtggatcgc tcatcccgag cggacgctat cggagcgagg atcgcgatgt
     3061 catggctcgt tcacgtggtc ggggtgacga gctccgaggg cgcgacgccc ggcgagatgt
     3121 gcgtggcctc ggcgctcgtc acgcgaccgt cctggacccc gtcgaagagg gagagaagga
     3181 ggccacctcg cgagaaacac cacaccaccc tgcggaccgc ttcggccgag ccaccagtcg
     3241 cgtggaagca ttcggctcgc atcccggcga tcgtgcgcgg cccttcggcc gtcaccgc
//
LOCUS       DATBRK010000085         6000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2610784, whole genome shotgun sequence.
ACCESSION   DATBRK010000085 DATBRK010000000
VERSION     DATBRK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2610784"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..725
                     /locus_tag="VIH70_02615"
     CDS             72..725
                     /locus_tag="VIH70_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY8115286.1"
                     /translation="MAERTFRDGARAVGPLAVAVVGFGISFGVLARAVGFGWLAPIVM
                     SATTFAGSAQFASVSVLGDGGTVAAAVAAALLLNARYAPIGISVAPYLTGSSWSRFVH
                     AQLVVDESWAIAAEGEGRFSRSVLLGAGVVLYMSWVAGTTVGALVGQGIGDPATFGLD
                     AAFAALFLGLLVPIARDRRHVAAALLGATIALALTPFAPAGVPIIAASAACLLGLKR"
     gene            722..1030
                     /locus_tag="VIH70_02620"
     CDS             722..1030
                     /locus_tag="VIH70_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY8115287.1"
                     /translation="MTQTWLVVGLVGAGTIAIKAIGPVLLGGRPLPERLSRVIELLAP
                     ALLGALVAVQTFGNGQALAVDERVLGVAVAAIAIWRKAPLLLVVILATVTTAFARALL
                     "
     gene            1056..1126
                     /locus_tag="VIH70_02625"
     tRNA            1056..1126
                     /locus_tag="VIH70_02625"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1088..1090,aa:Gly,seq:tcc)
     gene            complement(1370..2575)
                     /locus_tag="VIH70_02630"
     CDS             complement(1370..2575)
                     /locus_tag="VIH70_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8115288.1"
                     /translation="MTGFSKQDVADRAGVDRGYVDRLVELGILQPGGGDTYSPGDVRR
                     ARWVKGFERAGVPLEGIATAVRDGALSFSYLDATAFDRFAGVSRTKFRELSERTGVPM
                     DLLLVVREAHGFAEPGPEDHVREDELAIVPVMELLLSFGIRPEAIKRLLRAYGDGLRR
                     IVETETDWYRTEVELPLLEGGMTEVEMQAAQADVGSRISPLLDQTLVSIYHGQQEHAW
                     SKSAVEGVETALEQTGLYRRVRRPPAVSFLDITGYTRLTEERGDEAAAELATRLATLV
                     RGSSREHGGQPVKWLGDGVMFYFPDPGDGVLAALDMVEGVAAEALPPARVGIHAGPVV
                     FQEGDYFGRTVNIAARIADYARPGEVLVTQEVVDAADLDGVGFTTIGPIELKGVAEPL
                     NLRSVRRVG"
     gene            complement(2600..3061)
                     /locus_tag="VIH70_02635"
     CDS             complement(2600..3061)
                     /locus_tag="VIH70_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115289.1"
                     /translation="MKTCPYCAEEIKDAAIKCRWCGSWLVDEIPAAAVTPPKRVEAPA
                     AQPTLEPAATPQPEAEAPAAAAPEQAAPAAAAPVATAAPAPEAKIEFTHTGSRYLLGY
                     APDHFGIWDRNTPQQPVETFPRDDDGWAAAWARYATIETSWMDLRTGQKSG"
     gene            complement(3161..4390)
                     /locus_tag="VIH70_02640"
     CDS             complement(3161..4390)
                     /locus_tag="VIH70_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8115290.1"
                     /translation="MSDDLTRIRAGRLIDVVAGEVLRDRVIGIRGDRIESIGDDDGSP
                     VQIDLSEHTVLPGLIDCHAHLIGEIEGGHGYASLVQRSGAQEALTGVRNARDTVRAGF
                     TSVRDVGTFRAFVDVALRDAIEAGDVVGPRMLCAGAYVTSSGGGGDVTGLAPDVDAVV
                     PRELRFGVANSVDEVRRVVREILHGGADFIKVIATGAVLTEGTVPSAPEFSEAEIRAA
                     VEEAAVYETHVAAHAHGAEGIERAVRAGARSIEHGSLMDDDSIALMAEHGTYLVADVW
                     MGDWLSDQGEREGWSPDVMRKLRETTDVQREGFAKAVEAGVRIAFGTDSGTYPHGMNA
                     HQFATMVKLGMTPIEAIRSATVVAAELVGWRDRVGAIEPGLFADLVGVRGDVLSDVGL
                     LTDVDVVMKGGVLLPAG"
     gene            complement(4387..5946)
                     /locus_tag="VIH70_02645"
     CDS             complement(4387..5946)
                     /locus_tag="VIH70_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8115291.1"
                     /translation="MVRTVASLIGGRARDDAPGGTRDIPNPARLDEIVATVRLADATT
                     FVEACAAARNVQRDWARVPAPVRGRAVQQLGRLVEDNAETLARTMTREIGKPIAEARG
                     SVQEVVDTCNFFVSEGRRLYGMTVPSELPDKQLFTFRNPVGVAAIVTAGNFPVAVPSW
                     YLVPALLCGNAAVWKPAEYAPATSEALARLFLHAGFPDGVFNMVLADGAATFEGLSDA
                     LDAGLIDKVGFTGSSDVGRRLGELCGRHLQTPCLELGGKNPLLVMPDAALDLAVEGAL
                     FSGFGTAGQRCTSLGTVIVHEQVHDAFLARFAEAVETAPIGDPFEDVLYGPMISSRFR
                     DRFLGWLDWVQPHHTLHGSNATGRITRADPRAGFVGDPDAGLYVHPTIVDGVTMDDDL
                     YKQETFGPIVGVASFGDFEEAMALANGHGYGLSASIYTTDPLNVFRFRERISAGMVSV
                     NNSTTGAEAHLPFGGNGRSGNGARQSGVWVLDQFTRWQAMNWDYSGKLQKAQMDVADV
                     SADPSFRLTEP"
BASE COUNT          833 a         2171 c         2063 g          933 t
ORIGIN      
        1 gtgaacacgt cgacgagccg gaagggaacg ttcacgccgg gcactgtacc gacccattgc
       61 taccgttggt catggccgaa cggaccttcc gggacggggc tcgagccgtc ggcccgctcg
      121 cggtcgccgt cgtcgggttc gggatctcgt tcggcgtgct cgcgcgggcg gtcgggttcg
      181 gctggctggc cccgatcgtg atgtcggcga cgacgttcgc ggggtcggcc cagttcgcat
      241 cggtctcggt cctgggggac ggcgggacgg tcgccgcggc cgtcgcggcg gcgctgctgc
      301 tgaacgcgag gtacgcgccg atcggcatct cggtcgcgcc gtacctgacc ggttcctcgt
      361 ggtcgcggtt cgtccacgcg cagctcgtgg tcgacgagtc gtgggcgatc gccgccgagg
      421 gagagggccg cttctcgcgg agtgtgttgc tcggtgccgg tgtcgtcctg tacatgtcgt
      481 gggtcgccgg gacaacggtc ggcgcgctgg tgggacaggg gatcggcgac cccgccacct
      541 tcgggctcga cgcggcgttc gccgccctgt tcctcgggct cctcgtgccg atcgcgcggg
      601 accgtcgtca cgtcgcggcg gcgttgctcg gggcgacgat cgcgctcgcg ctcacgccgt
      661 tcgcgccggc cggcgtgccg atcatcgcgg cgagcgccgc gtgcctgctg ggcttgaagc
      721 gatgactcag acgtggctcg tcgtcgggct ggtgggcgcc ggcacgatcg cgatcaaggc
      781 gatcggcccg gtgctgctcg gcggccgtcc gctccccgag cggctctcgc gcgtgatcga
      841 gctgctcgcg cccgccctcc tcggcgcgct cgtcgcggtg cagacgttcg gaaacgggca
      901 ggcgctggcg gtcgacgaac gcgtcctcgg ggtggcggtc gcggcgatcg cgatctggcg
      961 gaaggcgccg ctcctgctcg tcgtgatcct ggcgaccgtc acgacggcgt tcgcccgggc
     1021 gctgctctga ggttgctagg cttggctgaa cgcaggcggg cgtagctcaa tggcagagct
     1081 ccagccttcc aagctggtgg tgcgggttcg attcccgtcg cccgctccgc ccgtcgacgc
     1141 ccttgcgggg tcgaaatggc acggcagctc gttgaagctc gacggcgaca tcttcccgtg
     1201 cgagtggcac ggctctacgt tcgacgccag gacgggcagg gcgattttcg ggaccggttc
     1261 gccccaacgc caggctgatc atgctgccga cccgcgtcca gatggcgtcc tgacctacgt
     1321 cttcgaggag cgttccgaga cctgagctga tgccgcgcgt aggggctgct cagccgaccc
     1381 ggcgcaccga acgcaggttg agcggttccg cgacgccctt cagctcgatc ggtccgatcg
     1441 tggtaaagcc aacgccgtcg aggtcggccg cgtccacgac ctcctgagtc acgagcacct
     1501 cgccgggtct ggcgtagtcg gcgatccgcg ccgcgatgtt gacggtccgg ccgaagtagt
     1561 cgccctcctg gaagacgacc ggcccggcgt ggatcccgac cctcgcaggc gggagggcct
     1621 ccgcggccac gccctccacc atgtccagag cggccaacac gccgtcaccc gggtccggga
     1681 agtagaacat cactccgtcg ccgagccact tgaccggctg tcccccgtgt tcccgcgagg
     1741 acccgcggac cagggtcgcc agcctcgttg ccagctcggc ggcggcctcg tctccccgct
     1801 cctcggtcag acgcgtgtac ccggtgatgt cgaggaagga cacggccggc gggcggcgga
     1861 cccggcggta cagaccggtc tgttcgagcg cagtctccac gccctccacc gcgctcttgc
     1921 tccacgcgtg ctcctgctgc ccgtggtaga tcgacaccag ggtctggtcc aggaggggtg
     1981 agatccggga cccgacgtcg gcctgggccg cctgcatctc gacctccgtc atcccccctt
     2041 cgaggagcgg cagctcgact tcggtcctgt accagtccgt ctcggtctcg acgatcctgc
     2101 gaaggccgtc tccgtaggct cggagcaatc gcttgatcgc ctccggtcgg atcccgaagg
     2161 acagcaagag ctccatcaca ggcacgatcg ccagctcgtc ctcccggacg tgatcttcgg
     2221 gccctggttc ggcaaagccg tgtgcctccc gaacgaccag gaggaggtcc atgggcacgc
     2281 ccgtccgttc gctcagctcg cggaacttcg tccggctcac ccccgcgaac cgatcgaagg
     2341 cggtcgcatc cagataggag aacgaaagtg ctccgtcccg cacggcggtg gctatcccct
     2401 cgagtggcac cccggcccgt tcgaagccct tcacccaccg ggcccgacgg acgtcaccgg
     2461 gcgagtacgt gtcaccgcca ccgggttgca ggatcccgag ctccaccaac cggtcgacgt
     2521 agcctcggtc gaccccggcg cgatcggcga cgtcctgttt cgagaatccg gtcacgatcg
     2581 gctcaggcca cgccgagcct cacccggact tctgacccgt tcggagatcc atccagctcg
     2641 tctcgatcgt cgcgtagcgg gcccacgcgg cggcccaccc gtcgtcgtcg cgcgggaacg
     2701 tctcgacggg ctgctgcggc gtgttgcggt cccagatccc gaagtgatcg ggtgcgtacc
     2761 cgagcaggta ccgcgaaccc gtgtgcgtga actcgatctt ggcctcgggc gcgggtgccg
     2821 cggttgcaac aggtgcggcg gccgcgggcg ccgcctgctc gggagccgcc gcggccggtg
     2881 cctcagcttc gggctgcggg gtggcggcgg gctcgagcgt cggctgggcg gccggggcct
     2941 cgacacgctt gggcggcgtc accgccgcgg ccgggatctc gtcgacgagc cacgatccgc
     3001 accaacggca cttgatcgcc gcgtccttga tctcctcggc gcaatacgga caggtcttca
     3061 tcgaggtgct ccttccgcgc ttttgtcact ccccgcacag cttttcgaca ggctcgacag
     3121 cgtactccgg gaccccgaga cgagggaacc cgggtgggtg tcagcccgcc ggcaggagca
     3181 cgccgccctt catgacgaca tccacgtccg tgagcaggcc gacgtcgctc agcacgtcgc
     3241 ctcggacgcc cacgaggtcg gcgaacagcc ccggctcgat cgcgccgacc cgatcgcgcc
     3301 acccgacgag ctcggcggcc accacggtgg cagagcggat cgcctcgatc ggcgtcatcc
     3361 cgagcttgac catggtggcg aactggtggg cgttcatccc gtgcgggtag gtcccgctat
     3421 cggtcccgaa cgcgatccgg acccccgcct cgacagcctt ggcgaacccc tcccgctgca
     3481 cgtcggtcgt ctcgcggagc ttgcgcatca cgtccggcga ccagccctcg cgctcgccct
     3541 ggtcgctcaa ccagtccccc atccagacgt ccgcgaccag gtaggtgccg tgctcggcca
     3601 tgagcgcgat cgagtcgtcg tccatcagcg agccgtgctc gatcgagcgc gcgccggcgc
     3661 gaaccgcgcg ctcgatgccc tcggcgccgt gggcgtgggc cgcgacgtgg gtctcgtaca
     3721 ccgcggcctc ctcgaccgca gcccggatct cggcctcgct gaactcgggc gcggacggga
     3781 cggtgccctc ggtaaggacc gcaccggtcg cgatgacctt gatgaagtcg gcgccgccgt
     3841 gcaggatctc gcgcaccacc cgccgcacct cgtcgaccga gttcgcgacg ccgaaccgca
     3901 gctcgcgggg cacgaccgcg tcgacgtcgg gggcgagacc ggtcacgtcg ccgcccccgc
     3961 ccgagctcgt cacgtacgcc ccggcacaca gcatccgagg tccgaccacg tcgccggcct
     4021 cgatcgcgtc gcgcagcgca acgtcgacga aggcgcggaa cgtcccgacg tcccgaacac
     4081 tcgtgaaccc ggccctcacc gtgtcgcggg cgttccggac gccggtgagg gcctcctgcg
     4141 cgccactccg ctgcacgagc gaggcgtacc cgtgcccgcc ctcgatctcg ccgatcaggt
     4201 gcgcgtggca gtcgatcaac cccgggagca cggtgtgctc ggacaggtcg atctgcaccg
     4261 gcgatccgtc gtcatctccg atcgactcga tccggtcgcc gcggatgccg atcacgcgat
     4321 cgcggagcac ctcgcccgcg acgacgtcga tcagccgtcc ggcgcggatg cgcgtgaggt
     4381 cgtcgctcat ggctcggtca gacggaacga cgggtcggcc gacacgtcgg cgacgtccat
     4441 ctgcgccttc tgcagtttgc ccgagtagtc ccagttcatg gcctgccagc gcgtgaactg
     4501 atcgagcacc cacaccccgg actgtcgcgc gccgttgccg ctgcgtccgt tgccgccgaa
     4561 gggcaggtgg gcctcggctc cggtggtcga gttgttcacg ctcaccatgc ccgccgagat
     4621 gcgctcgcgg aaccggaaga cgttgagcgg gtccgtcgtg tagatcgagg ccgacaggcc
     4681 gtagccgtgg ccgttcgcga gagccatcgc ctcttcgaag tccccgaacg aggcgacgcc
     4741 cacgatcggg ccgaacgtct cctgtttgta caggtcgtcg tccatcgtca cgccgtcgac
     4801 gatcgtcggg tgcacgtaca gaccggcatc cgggtcgccg acgaagccgg cgcgagggtc
     4861 ggcgcgtgtg atcctgcccg tcgcgttcga accgtggagc gtatggtgcg gttgcaccca
     4921 gtcgagccac ccgaggaacc tgtcgcggaa acgcgacgag atcatcggcc cgtagagcac
     4981 gtcctcgaac ggatcgccga tcggcgcggt ctcgaccgcc tcggcgaacc gtgcgaggaa
     5041 cgcatcgtgg acctgctcgt gcacgatgac cgtcccgagc gaggtgcagc gctgccccgc
     5101 ggtcccgaac ccgctgaaca gcgcgccctc gaccgcgagg tcgagggcgg cgtcgggcat
     5161 cacgaggagc gggttcttcc cgccgagctc gagacagggc gtctgcaggt gtcggccgca
     5221 gagctcgccg agcctccgcc ccacgtcgct cgaacccgtg aagccgacct tgtcgatcaa
     5281 ccccgcgtcc agggcatccg agagcccctc gaacgtggcg gcgccatcgg cgagcaccat
     5341 gttgaacacg ccatcgggga aaccggcgtg caggaacagc cgagcgagcg cctcggaggt
     5401 ggcgggcgcg tactcggcgg gcttccacac cgcggcattg ccgcacagga gtgccgggac
     5461 caggtaccag gagggaaccg cgacggggaa gttccccgcg gtcacgatcg ccgcgacgcc
     5521 gaccgggttg cggaacgtga acagctgctt gtccgggagc tcggacggca ccgtcatccc
     5581 gtagagacgt cggccctccg acacgaagaa gttgcacgtg tcgacgacct cctggacgga
     5641 gcctcgggcc tcggcgatcg gcttgccgat ctcgcgcgtc atggtgcggg cgagcgtctc
     5701 ggcgttgtcc tcgaccaggc gcccgagctg ctggaccgcg cgcccgcgca ccggcgccgg
     5761 gacgcgggcc cagtcgcgct gcacgttccg ggctgcggcg caggcctcga cgaaggtcgt
     5821 cgcgtcggcc aatcggacgg tcgcgacgat ctcgtcgagc ctggcggggt tcgggatgtc
     5881 ccgggtgccg cccggcgcgt cgtcgcgcgc tcgacccccg atcaacgaag cgacggtgcg
     5941 taccaccctg cctccacgct cggagcaccc gatagtagcg gcccggccgc ccccgggacg
//
LOCUS       DATBRK010000086         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_155782, whole genome shotgun sequence.
ACCESSION   DATBRK010000086 DATBRK010000000
VERSION     DATBRK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155782"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VIH70_02650"
     CDS             complement(<1..263)
                     /locus_tag="VIH70_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115292.1"
                     /translation="MLTAEQIGQFHRDGVLALRGVFDRDEVRALQRAADEVTAEAVAG
                     RGHGHGYREVDGRRQYYRTDGVLWDHDAAFRMATVNPILLSAVG"
     gene            374..1204
                     /locus_tag="VIH70_02655"
     CDS             374..1204
                     /locus_tag="VIH70_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8115293.1"
                     /translation="MTGAPRGSSPTVVEAGVHRPGLMRRHADRLLPVHELIVVQAGML
                     PIAEEDRRFEVRRNEWVLLRAGRRHSGYDDLDGKTWFYWVCFGDSGDAEVDDAISERR
                     TGTVSRPGRVRAHFEHLLEDQHRAILSPTTARSHLQLLLAEMRLPPIAEARDETAPGL
                     AERASAFVGEHLSEPDLSTARVAGALACSPDYLGRVFRASFDETLTDHIHRLRIERAL
                     TLFRSTGLPADRVAAAVGFGDVRYFRRIFKRRVGLTSGQFRRLRPSAGDGTAEALHPS
                     "
BASE COUNT          382 a          879 c          796 g          412 t
ORIGIN      
        1 ccgactgccg agagcaggat ggggttcacc gtggccatcc ggaacgcggc gtcgtggtcc
       61 cacagtaccc cgtcggtgcg gtagtactgc cggcggccgt cgacctctcg atagccgtgc
      121 ccgtgcccac ggccggcgac cgcctccgcc gtcacctcgt ccgcagcgcg ctggagcgct
      181 cggacctcgt cccggtcgaa gacgccgcgc agggcaagca ccccatcgcg gtggaactgc
      241 ccgatctgct cggcggtgag catcttcgtc ctccccccct tgtgactcga ttccgacctc
      301 gcgccgacgc taaccaggcc ggatacctgg ggcgagggcc ggttccgacg tcgaggggta
      361 cgattccgtc ctcatgaccg gagcaccgcg cggatcgtca cccaccgtgg tggaggcggg
      421 cgtgcatcgg ccgggactga tgaggcgaca cgccgaccgc ctcctgcccg tccacgagct
      481 catcgtggtg caggcgggga tgctgcccat cgcggaggag gaccggcgtt tcgaggttcg
      541 ccggaatgag tgggtcctgc tgcgggcggg ccggcggcac tccgggtacg acgatctcga
      601 cggaaagacg tggttctact gggtgtgctt tggcgactca ggggatgcag aggtcgacga
      661 cgcgatttcg gagcgccgga ccggcacggt ttcacggccc ggccgggtgc gcgcccattt
      721 cgagcatctc cttgaggacc agcaccgggc gatcctgtcg ccgacgaccg ctcgcagcca
      781 cctgcagctc ctcctggccg agatgcggct cccgccgatc gccgaggccc gcgacgagac
      841 cgcgccgggt ctcgccgagc gcgcatcggc cttcgtcggg gagcatctga gcgagccgga
      901 cctgagcacc gcccgcgtcg cgggcgccct ggcctgcagt cccgactacc tgggacgcgt
      961 cttccgagct tcgttcgacg agacgctcac ggaccacatc caccggctgc gcatcgaaag
     1021 ggcgctgacg ctgttccgct cgacgggact gccggcggat cgcgtcgccg cggcggtcgg
     1081 gttcggcgac gtgcgctact tccgccggat cttcaagcga cgggtcgggc tcacctcggg
     1141 gcagttccgg cgcctgcgtc cgtccgcggg ggacgggacg gccgaggcgc tgcacccgag
     1201 ctaggaaccc ttgtttcgtt tgatcacgaa cgtcacgaac gacagctctg tccgatggac
     1261 gctgcccgtg atctcccaaa tccctcggtg ggaaagatca caccgctcgg ccggaacccg
     1321 cgggggccgt atccgtcggg aacaaccccg cgaaccggcg gggcaggccg gtccagtcgg
     1381 cggccggtga tgttgagccg gcccggcacc ttcgaccacc atccgaactt catccccacg
     1441 cgccctcgat cgtcaacgaa gccgtggccg acggcgagga tcccgtgcgg ccagagtcct
     1501 ccgacccaaa gcgtgccgtt gccgtaggag gctccccatc cgaagaaggc gtctcgtgcc
     1561 gggcccgaag ggccggccgg atttgggagc gtgactgggc aggccttcgc ctgggccatc
     1621 gtgaccggtc cggatgtctc atcgaccgcc gaccccgtca acggcggcga cgtgcccgga
     1681 gcgcccgact catcgagcct ccccttgggt gtacaagtca ccagggccat cgcgacaccg
     1741 atggcgaaga ttcgtgcgag tctcacagac gagcgctccc ctgaccgatc ccatgctgtg
     1801 ttacaccctt cggcgccgat tggttccgct gcacgtgctc ttccatgcct tgttagctac
     1861 acctcaccaa gcttcaggac cgctcgcgcc atgaaacggt gttttaggat ttgatctgga
     1921 cccgagagcc gttgggaact cgcgcgtcca cccatggtga cgttgcttcg acccgaagac
     1981 ccagggacgc cacgatgcgt tcgatcacgt ggcggatcgc ctcctccttc caccctgatc
     2041 gccagccttc cccaatggct ccgcggattc tccccggccg tcttctcgtg cctccgccaa
     2101 cgtgtcgcgt cgtagggaca ctgttacggg tcggggcccg tctacctgcc cggcgtcgaa
     2161 gtcgccgtgg ggcgcgagct cccggtgagc tcattattga gcacatcggc ctgaggaacg
     2221 gtgttcggca tgcgccgtcg accatagggg ttcaaagacc ccaaccgaca cgatctggct
     2281 gaaacgttgg gtcagtagat cccgcccgag ccaccaccac ccgggcctcc gccggcattc
     2341 gcggcgatga gaacgatgag tccggcaacg accgctaggc cgacaacgat tccgacccat
     2401 accttccagg tccaaccact gcgcggctgc atgctcctcg agttgtccat gacggacctc
     2461 cttcggctc
//
LOCUS       DATBRK010000087         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1887209, whole genome shotgun sequence.
ACCESSION   DATBRK010000087 DATBRK010000000
VERSION     DATBRK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887209"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1533)
                     /locus_tag="VIH70_02660"
     CDS             complement(202..1533)
                     /locus_tag="VIH70_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010793081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEY8115294.1"
                     /translation="MVARFDGGTISSDAGGLLLRETDRRIRLLKRLRDCFRDGRSPGR
                     VEHGLEQMLGQRIYALALGYEDLNDHEQLREDPLLAVLAGKSDPTGQDRKRQRDKGKP
                     LAGKSTLNRLEHGIATPDRYKKVNYAAEAIDRLLVDLWLEAHTQAPEEVILDLDTTDV
                     ALHGHQEGRFFHGYYNHYCYLPLYIFCGEHLLCARLRPSNRDASAGSLKEIQAIVAQM
                     RAVWPEVRIILRADSGFCREELMSWCETHAVDYVFGLARNERLRAQIESEMQAAAGLY
                     AATGKPARVFTEFFYQTHKSWSRSRRVVAKAEQIEGKENPRFVVTSLSPHGWAAAALY
                     EQLYCARGDMENRIKEQFSLFSDRLSTATMRANQFRLYLSSLAYVLLQALRRLGLRGT
                     ELAHAQASTLRLKLLKIGAQIRVTVRRVWVAMATSYAHRRLFETVYQQLRC"
BASE COUNT          311 a          487 c          435 g          325 t
ORIGIN      
        1 ttaaccgttc cttctcacca atgagaagaa ggtagcgaaa atggtagtca gctggcgcga
       61 cgggtgaaga gggcaatggc gcacggctgg aaactactgc aagagctttc cgcgggggag
      121 cagaaacgaa tttttggcaa agctcgcgct gactgctcgt ttgctttccg gattttgtgg
      181 atagctggtc ctgctccgag gtcagcagcg tagttgttgg tagacggttt cgaacaagcg
      241 tcggtgggca tagctggtgg ccatcgctac ccagactcgg cgcaccgtga cccgaatctg
      301 ggctccgatc ttcagtaatt tgaggcgtag cgtggaagct tgcgcgtgcg ccagttctgt
      361 accccttaaa cccaaacggc gcagagcctg cagcagtaca taggctagcg aagagagata
      421 cagccggaat tgattggcgc gcatggtcgc cgtgctgagc cgatcgctga acagggagaa
      481 ctgttccttg atgcgattct ccatgtctcc tcgggcacag tagagctgct catagagagc
      541 ggccgcggcc cagccgtgtg gcgacagtga ggtcacgacg aagcgcgggt tctctttgcc
      601 ctcaatctgc tcagccttgg ccaccacccg gcggctccgg ctccaactct tgtgcgtctg
      661 atagaagaac tcggtgaaca cccgtgccgg ctttcccgtc gccgcatata gccccgccgc
      721 cgcttgcatc tcgctttcaa tctgggcccg cagacgttca ttgcgcgcca agccaaacac
      781 gtagtccacg gcatgggttt cgcaccaact catcagctcc tcgcggcaga aaccggaatc
      841 ggcgcgcagg atgattctta cttccggcca caccgcgcgc atctgcgcta cgattgcttg
      901 gatctctttc aggctacccg cggaagcatc gcggttggac ggccgcaggc gcgcacacag
      961 cagatgctcc ccgcaaaaaa tgtagagcgg caaatagcag tagtggttgt aatacccatg
     1021 gaagaagcgg ccttcctggt gtccgtgcag cgccacatcg gtcgtgtcga gatcgagaat
     1081 gacttcttcc ggagcctggg tgtgcgcttc cagccacaga tccactaaca agcgatcgat
     1141 ggcctccgca gcgtagttca ctttcttgta gcgatcgggt gtcgctatcc cgtgctccaa
     1201 gcgattcagt gtgctcttgc ccgccaacgg cttgcccttg tcccgttgcc gttttcgatc
     1261 ctgcccggtg ggatcgctct ttcccgccaa caccgccaac agcggatcct cccgcaactg
     1321 ctcgtgatca ttgagatctt catagcccag cgccagcgca taaatgcgct gccccagcat
     1381 ctgttccagc ccgtgctcga cccgccccgg actccgtcca tcgcggaaac aatcccgcag
     1441 tcgcttcagc aatcggatcc gtcggtcggt ctctcgcagc agcaatcccc cggcatcgga
     1501 actgatcgtt ccaccgtcga agcgcgccac cacctggcga gaaaaaagct cttcaaac
//
LOCUS       DATBRK010000088         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_731709, whole genome shotgun sequence.
ACCESSION   DATBRK010000088 DATBRK010000000
VERSION     DATBRK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731709"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="VIH70_02665"
     CDS             <1..1431
                     /locus_tag="VIH70_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY8115295.1"
                     /translation="AGREVADAAVRLIGGAYGRRAVVVCGKGNNGGDGLVAARYLDRA
                     GIRTTVLLLDDAPAGPAGTNLARLGTTTARRRAFEPAALDRELGRADVEVDAIFGTGF
                     RGAPTGSYAEAIGALAASPIPLVAVDIPSGVNGETGAVQGPAVRADLTVTFGAPKPGL
                     VLLPGATRAGVVEVQQIGFPADLIRSDLVLLEAEDIAAMLPSREPDTHKRSAGYAVVI
                     GGSRLMTGAVALAGTAAYRSGAGLVAVAVPEGILPVSQGAVREAVFVGLPETDAGTAA
                     GGSARLDEVLGQADALAVGPGMTTDERTAGWVRELVRSSEVPVVLDADGLNAFAGRAE
                     DLADRKADLILTPHAGEFARVAGVSMGDVAADRIRHVRELAARTSATVLLKGSRTLIG
                     APDGSVRINPTGGPFLATGGTGDVLTGMIAALLARGCSPLDAGSAAAFVHGLAGGLAG
                     EERGVGTTASDVLERVPTVMREVVGG"
     gene            1428..2552
                     /gene="alr"
                     /locus_tag="VIH70_02670"
     CDS             1428..2552
                     /gene="alr"
                     /locus_tag="VIH70_02670"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY8115296.1"
                     /translation="MTRYRPTVVEIDLEAIAHNVRRLRPPAAELMAVVKADGYGHGDA
                     EVAKAALEAGASRLGVSLVEEGLGLRDRGIDAPVLVLAEFPPGSEKEALERGLTPTVY
                     TDEGVAGVAEAARAIGRPVGVHLKLDSGMHRVGLWPPDRAPAFARRVLDAGLELEGLW
                     THFADSELDEAGTLEQLALFRAAADALAAVGITPGVLHAANSGATIGLPRTHLDLVRP
                     GAAVYGIDPGGGLAEPFGLRPALSWRSAVTMVKRLPAGERLSYGGRYALERDATIATV
                     PVGYDDGFPRQLGGMGEVLIGGRRHPVVGTITMDQILVDCGDDSVSPGDEVVLIGTQG
                     DERITVEELAERAGTIARQIATGIGARVPREYPAADGRPR"
BASE COUNT          322 a          910 c         1064 g          351 t
ORIGIN      
        1 gccggccgcg aggtcgccga cgccgccgtc cggctgatcg gcggcgcgta cgggcggcgc
       61 gcggtggtgg tgtgcggcaa gggcaacaac ggtggcgacg gtctggtcgc ggcccggtac
      121 cttgaccgcg ccggcatccg gaccacggtg ctgctgctgg acgatgcgcc ggcgggtccc
      181 gcggggacca acctcgcgcg actcggaacc acgacggcgc ggcgtcgcgc gttcgagccg
      241 gcggcgctcg atcgcgagct cgggcgcgcc gacgtggagg tcgacgcgat cttcgggacg
      301 ggcttccgcg gggccccgac cgggtcgtac gccgaggcga tcggggcgct cgccgcgtcc
      361 ccgatcccgc tggtggccgt cgacatcccc tcgggcgtga acggcgagac cggggcagtg
      421 cagggtcctg cggtccgcgc cgatctcacg gtcacgttcg gggcgccgaa gcccgggctg
      481 gtcctgctgc ccggagccac gcgggcgggc gtcgtcgagg tccagcagat cgggttcccg
      541 gcggacctga tccgcagcga cctcgtgctg ctcgaggccg aggacatcgc ggcgatgctg
      601 ccgtcacgtg agcccgacac gcacaagcgc tcggcgggct acgcggtcgt gatcggcggc
      661 tcgaggttga tgacaggggc ggtcgcactc gccgggaccg ccgcgtaccg gtccggcgcg
      721 ggcctggtcg ccgtggccgt gcccgagggc atccttccgg tgtcgcaggg agccgtccgc
      781 gaggccgtgt tcgtcggcct ccccgagacc gacgcgggga cggcggccgg cggctcggct
      841 cgcctcgacg aggtgctcgg gcaggcggat gcgctcgccg tcgggccggg gatgacgacc
      901 gacgagcgca cggcgggatg ggtccgcgag ctggtgcgtt cgagcgaggt gccggtcgtc
      961 ctggacgcgg acggcctgaa cgcgttcgcg gggcgcgccg aggatctcgc ggatcgcaag
     1021 gccgacctga tcctcacgcc gcacgccggc gagttcgcgc gggtcgctgg ggtctcgatg
     1081 ggcgatgtgg cggccgatcg gatccgtcac gttcgcgagc tggccgcgag gacctcggcg
     1141 acggtgctcc tgaagggcag ccgaacgctg atcggcgcac cagacgggtc ggtccggatc
     1201 aacccgaccg ggggtccgtt cctcgcgacc ggcgggacgg gcgacgtgct caccgggatg
     1261 atcgcggccc tgctcgcgcg ggggtgttcg ccgctcgatg cgggctcggc ggccgcgttc
     1321 gtccatggcc tcgccggcgg cctcgcgggc gaggagcgcg gcgtgggcac caccgcctcc
     1381 gacgtgctcg aacgcgtccc gacggtgatg cgagaggtcg tcggcgggtg acgcggtacc
     1441 gtcccacggt cgtcgagatc gatctcgagg cgatcgcgca caacgtccgg cggctccgac
     1501 ccccagcggc ggagctgatg gccgtcgtga aggccgacgg atacggacac ggcgacgccg
     1561 aggtggcgaa ggcggcgctc gaggcggggg cctcgaggct cggggtctcg ctcgtggagg
     1621 aggggctcgg gctgcgcgat cgtgggatcg acgcgccggt cctcgtgctg gccgagttcc
     1681 cgcccggctc ggagaaggag gcgctcgagc gcggactgac gccgaccgtg tacacggacg
     1741 agggcgtggc gggcgtggcc gaggccgctc gggcgatcgg ccgccctgtg ggtgtgcacc
     1801 tcaagctcga cagcgggatg catcgggtcg gtctgtggcc gcccgaccga gccccggcat
     1861 tcgcgcggcg tgtcctcgac gccgggctcg agctcgaggg cctgtggacc cacttcgccg
     1921 actcggagct ggacgaggcg gggacgctcg agcagctggc gctgttccgc gcggccgccg
     1981 acgcgttggc ggcggtgggg atcacgccgg gggttctcca cgcggcgaac agcggcgcca
     2041 cgatcggact cccgcggacc catctcgacc tcgtgcgccc cggcgcggcc gtctacggga
     2101 tcgaccccgg cggcgggctc gccgagccgt tcggcctgcg gcccgccctg tcctggcgtt
     2161 cggcggtcac gatggtgaag cgattgcccg ccggcgagcg cctctcctac ggaggtcggt
     2221 acgcgctcga acgcgacgcc acgatcgcga cggtgccggt cgggtacgac gacgggttcc
     2281 cgaggcagct gggagggatg ggcgaggtct tgatcggggg gcgtcgccat ccggtcgtcg
     2341 gaacgatcac gatggaccag atcctcgtcg actgcgggga cgactccgtg tctcccggcg
     2401 acgaggtggt gctgatcggc acccagggcg acgaacgcat cacggtcgag gagctcgccg
     2461 aacgcgcggg cacgatcgcg cgccagatcg cgaccgggat cggcgcacgc gtcccgcgcg
     2521 agtatccggc cgcggacgga cgaccccgat gagccgcaag cgcacggccc tggtcaccgc
     2581 cggtatcgcg gccggcgcgg ttgcgggcgg cgtgatcggg cgcaccgtgc tgaacgcgcg
     2641 acgccgg
//
LOCUS       DATBRK010000089         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2464426, whole genome shotgun sequence.
ACCESSION   DATBRK010000089 DATBRK010000000
VERSION     DATBRK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464426"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VIH70_02675"
     CDS             complement(<1..270)
                     /locus_tag="VIH70_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY8115297.1"
                     /translation="MSAAFSIDNPNRITATVPPGATDGPIAVTNTDGTATSAASFDVA
                     TNFTVTAGGFSITSFNPTSGVIGTSVVITGVGFTGANAVRFNGTTA"
     gene            complement(656..2215)
                     /locus_tag="VIH70_02680"
     CDS             complement(656..2215)
                     /locus_tag="VIH70_02680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8115298.1"
                     /translation="MRILLWHGWLLEGSGSNVATARVAEHYRAAGHDVLLLCQERHPE
                     RYPWIDAFGSVDAGGPSELTWQAVRGASGRCVVLRPRIGSMIPVFVVDRYEGFDEVRR
                     FVDLSDDDLGSYLRANVDALRAAAAWHGTEFTFTGHAIPGAAIGRRALGVGRYVAKIH
                     GSDIEYAVRIQERYRELAREGLRAARAVVGPTADVLARCSALVPGIEELARIVPPGVE
                     ISAFRPRPRHEALLEAAELLERDPDTARGRPSSIDEQVERALDDREAVALDALAENYD
                     QAVPEPDAAARLRRLAGTSGPIVGYLGKLIPQKGVELMLQAHRTADHEAHALVVGFGS
                     YREWLAALAIALERGDAEAVAWMRDVREIPIEAGPDPGPRTGERDVTFTGRLDHRYAP
                     DALAAMDVMVVPSILSEAFGMVAAEGAAAGALPLVARHSGLGEVGAALEAAIGRPGLL
                     TFEPGPGAVGRLATALDRLLELPPAERDELRGTVSAFVGREWTWDRTASALLAAAGLD
                     SPDRRAYADPP"
     gene            complement(2290..>2482)
                     /locus_tag="VIH70_02685"
     CDS             complement(2290..>2482)
                     /locus_tag="VIH70_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115299.1"
                     /translation="VRVDFYRTREGKTLRIGESDDGMLSVEILKDGAWTAAPLGMIGL
                     RLSPETRRLKASEIKILPN"
BASE COUNT          364 a          931 c          845 g          342 t
ORIGIN      
        1 cgccgtggtt ccgttgaagc ggaccgcgtt ggcccccgtg aagccgacgc ccgtgatgac
       61 caccgaggtt ccgatcacgc cgctcgtcgg gttgaacgat gtgatcgaga agcctccggc
      121 cgtcacggtg aagttggtcg ccacgtcgaa gctcgccgcg ctcgtcgccg tcccgtccgt
      181 gttcgtcacg gcgatcggtc catcggtcgc ccccggcggc accgttgccg tgatcctgtt
      241 cgggttgtcg atcgagaatg cggcggacac cccccttgaa cgtcaccgcg gtgacgtccg
      301 ggtcggggcc ggtgaagttg tcgccgttga tcacgacggg agcgccgatc gggccgctcg
      361 tcggcgtgaa cgaggagatc gcaggggccg ccgcgagggc cgcgtccgcg cccgggatgg
      421 cgatcgtgag ccccgccaga acgacggcca acgaggctcg caccgcgcga cgtaggaggt
      481 ccttcaagac gtccccctcc tcgggaccga aaggaaccgc ccctcgtcct ccctcatttc
      541 caccggatcc gcgatcgacg tgagggagcc ccgtcgggtc gactccacac gccgccaagc
      601 gctcggaccg tccacggccc tataaccacg ccgatggtct cggggttcct tcagctcacg
      661 gtgggtcggc gtaggctcgg cgatccgggg agtccaggcc cgcggccgcg agcagcgcgg
      721 acgcggttcg gtcccaggtc cactcgcgac cgacgaacgc gctcacggtc ccacggagct
      781 cgtcgcgctc cgcgggcggg agctcgagca agcgatcgag cgccgtcgcc agtcgcccca
      841 ccgccccggg gccgggctcg aacgtgagca gacccggacg gccgatcgcg gcctcgagcg
      901 ccgcgccgac ctctccgagg cccgagtggc gcgcgaccag cgggagcgcg ccggcggccg
      961 cgccctcggc ggccaccatc ccgaacgcct cggacaggat cgacggcacc accatcacgt
     1021 ccatcgcggc gagggcgtcg ggggcatacc gatgatcgag acggcccgtg aacgtgacgt
     1081 cgcgctcacc ggtccgcgga ccgggatcgg gtccggcctc gatcgggatc tcgcggacgt
     1141 cgcgcatcca cgcgaccgcc tcggcatcgc cgcgctcgag cgcgatagcg agcgcggcga
     1201 gccactcgcg atacgagccg aacccgacca ccagcgcgtg cgcctcgtgg tccgccgtcc
     1261 ggtgcgcctg gagcatcaac tcgacgccct tctgcgggat cagcttgccc aggtacccca
     1321 cgatcggccc gctcgtgccg gcgagccgtc ggagccgcgc cgccgcatcc ggctcgggca
     1381 ccgcctggtc gtagttctcc gcgagcgcat cgagcgccac ggcctcccga tcgtcgaggg
     1441 cccgctccac ctgctcgtcg atcgacgacg gccgtccccg cgccgtgtcc gggtcccgct
     1501 cgagcagctc ggccgcctcc agcaacgcct cgtgtctggg tcgcgggcgg aacgcggaga
     1561 tctcgacgcc aggcgggacg atccgggcga gctcttcgat ccccgggacc agtgccgagc
     1621 accgcgcgag cacgtcggcg gtggggccaa cgacggcgcg cgccgcccgg aggccctcgc
     1681 gcgcgagctc acggtaccgc tcctggatcc gcaccgcgta ctcgatgtcg ctgccgtgga
     1741 tcttcgcgac gtaccggccc acgccgagcg cgcgtctgcc gatcgcggcg cccgggatcg
     1801 cgtgacccgt gaacgtgaac tcggtcccgt gccacgcggc ggccgcgcgc agcgcatcga
     1861 cgttcgcccg caggtaggaa ccgaggtcgt cgtccgacag gtcgacgaac cgacggacct
     1921 cgtcgaaccc ctcgtaccgg tcaacgacga agacggggat catcgagccg atccgaggtc
     1981 tgagcacgac gcagcgcccg gacgcgcccc gcacggcttg ccacgtcagc tccgacggcc
     2041 cgccggcgtc gaccgatccg aacgcgtcga tccacgggta gcgctcggga tgccgctcct
     2101 ggcacaacag cagcacgtcg tgaccggcgg cgcggtagtg ctcggccacg cgcgcggtcg
     2161 cgacgttgga tcccgaaccc tcgaggagcc acccgtgcca caggaggatg cgcatcgtcg
     2221 gatgctagac gagcgcgctc gacggctcgg cgaacgacac ccttgcggtg cagcctcggt
     2281 gcggatcgct cagttgggaa ggatcttgat ctccgacgcc ttcaatcggc gcgtctctgg
     2341 agagagtcgc aggccgatca tgccgagcgg ggcggcggtc caggcaccgt ccttcaggat
     2401 ctccacgctc agcatcccgt cgtcggactc cccgatccgg agcgtcttgc cctctctcgt
     2461 acggtagaag tccacgcgca ca
//
LOCUS       DATBRK010000090         3281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_731985, whole genome shotgun sequence.
ACCESSION   DATBRK010000090 DATBRK010000000
VERSION     DATBRK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731985"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /locus_tag="VIH70_02690"
     CDS             complement(<1..1928)
                     /locus_tag="VIH70_02690"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY8115300.1"
                     /translation="MSLEQALDALIGSEAFERLLLERARPLVARADAGEDALVAALAR
                     ALGAPLLLVAPGPHEAERLARGARAWLGDDGAALLPSWEALPYEGISPSPEVSARRAE
                     AVRRLRAAKGAFVLVAPALAAMQGLVPTLGEIPPVELVKGIELPPDDLAERLVALGYA
                     RTDVVEHRGEFAVRGGVLDVFPGTARRPVRLEYWGDEIESLREFVPSSQLSSGPVVRV
                     EVSPVRELVPDDALAERARTRAPRYLDRFRDGLERLAEGLHAEGLESLAPLLFDRMPV
                     PAELLQEGSWVVVSEARRTFDRAHRAFEDAEALAEASNWPGPRAVHDLREALEGPVRL
                     ELTGFTEGIDTGLEGWGSAQGNPAELARRAAELAERGYRVLLTARGHGSLERVSEFVA
                     LPRAEPVEAELQAGFVFPAGRLAVATEEDLFGSRRHTRDAPRFTTRRGDAIAEELEVG
                     DFAVHRVHGVARYVGVQRRAIGGSERDYMVLEYASGDRLSVPTDQVGMVAKYVGGETP
                     RLSRLGTSDWARTTGRVKRAVKDMAGELVRLYSVRLSIERPPYGPDTPWQMELEDAFP
                     HEETGDQVTAIEEVKADLLRPRPMDRLICGDVGFGKTEIAVRAAFKVVMEGKQVAVLV
                     PTTLLAEQHLITFGERYAP"
     gene            complement(2041..2622)
                     /gene="pth"
                     /locus_tag="VIH70_02695"
     CDS             complement(2041..2622)
                     /gene="pth"
                     /locus_tag="VIH70_02695"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY8115301.1"
                     /translation="MTWLVAGLGNPGERYARTRHNLGYRVVDELVASERERFRKARFV
                     GADVSEIRVGGERVVLAKSRAFMNESGPAIASLARKHGVEPDHVIAVHDEIDLPFGAL
                     RVKSGGSTAGHNGLRSLQQALRTPDFFRVRLGVGRPTGRKQAADWVLDAFAKREEPDV
                     ALLVDDAADAVRSLIADGLLPTQDRYNRAPREP"
     gene            complement(2637..>3281)
                     /locus_tag="VIH70_02700"
     CDS             complement(2637..>3281)
                     /locus_tag="VIH70_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY8115302.1"
                     /translation="KGVARKLRAAGRVPGVLYGHGMDSTPLSVDAKDLFHVLHTGAGT
                     NVLVDLVVDGTEHLIIPRDVQRDHIHGRFIHIDFLAVRRDEKLHLSIPVRIVGESVGV
                     KAGGVVEHHLWELEVESLPGDVPEAIDADITSLEIGMSLRVSDLVPPKGVTILTNPEE
                     SVVAVQQPQMAVELEEEEAAAAEAEAAEGEAAEGEEGEAAQGEGAEGSDERSEG"
BASE COUNT          447 a         1242 c         1152 g          440 t
ORIGIN      
        1 ggcgcgtagc gctcgccgaa cgtgatcagg tgctgctcgg cgagcagggt cgtcggcacg
       61 agcaccgcca cctgcttgcc ctccatcacg accttgaacg cggcccgcac cgcgatctcg
      121 gtcttgccga accccacgtc gccacagatc aaccggtcca tcgggcgcgg ccgcagcagg
      181 tccgccttga cctcctcgat cgccgtgacc tggtcgccgg tctcctcgtg cgggaacgcg
      241 tcctcgagct ccatctgcca cggcgtgtcg gggccgtacg gcgggcgctc gatcgacagc
      301 cggaccgagt acagccgcac gagctcgccc gccatgtcct tcaccgcgcg cttcacgcgc
      361 ccggtcgtcc gcgcccagtc gctcgtccca aggcgcgaca ggcgaggcgt ttccccgccg
      421 acgtacttgg ccaccatccc cacctgatcg gtggggaccg agagccggtc gcccgaggcg
      481 tactcgagga ccatgtagtc gcgttcggaa ccgccgatcg cgcgacgctg cacgcccacg
      541 tagcgggcga ccccgtgcac gcggtgcacg gcgaagtcgc cgacctcgag ctcctcggcg
      601 atggcgtcgc cgcgacgcgt cgtgaaccgc ggggcgtcgc gcgtgtggcg ccgcgaaccg
      661 aacagatcct cctcggtcgc cacggcgagc ctgccggcgg ggaacacgaa gccggcctgc
      721 agctcggcct cgaccggctc ggcccgcggc agcgcgacga actcgctgac gcgttccagc
      781 gacccgtggc cgcgcgccgt gagcagcacg cggtacccgc gctcggcgag ctccgcggcg
      841 cgccgcgcga gctcggcggg gttgccctgc gccgagcccc acccctccag acccgtgtcg
      901 atgccctcgg tgaagcccgt gagctccagc cggaccggac cctcgagcgc ctccctcagg
      961 tcgtggaccg cgcgaggacc gggccagttc gaggcctcgg cgagcgcctc ggcgtcctcg
     1021 aacgcccgat gcgcccggtc gaatgtccgg cgggcctcgg ataccacgac ccacgagccc
     1081 tcctgcagca gctcggcggg gaccggcatc cggtcgaaca gcaggggggc gagcgactcg
     1141 agcccctcgg cgtgcagccc ctccgccaac cgctcgagcc cgtcgcggaa gcggtcgagg
     1201 tagcggggtg cccgcgttcg cgcgcgttcg gcaagcgcgt cgtcggggac gagttcgcgc
     1261 accggcgaca cctcgacccg gacgacgggc ccggacgaca gctgcgacga cggcacgaac
     1321 tcgcggagcg attcgatctc gtcgccccag tactcgaggc gcacgggacg ccgcgcggtc
     1381 ccggggaaca cgtcgagcac cccaccgcgg acggcgaact cgccgcggtg ctcgaccacg
     1441 tccgtgcgcg cgtacccgag ggcgaccagc cgctcggcga gatcgtccgg cgggagctcg
     1501 atccccttga ccagctcgac cggcgggatc tcgccgaggg tggggacgag gccctgcatc
     1561 gcggcgagag cgggggcgac caggacgaac gcgcccttgg ccgcgcggag ccggcggacc
     1621 gcctcggccc tgcgcgccga gacctccggc gaggggctga tcccctcgta cgggagcgcc
     1681 tcccacgagg gcaggagcgc cgccccgtcg tccccgagcc acgctcgtgc cccgcgggcg
     1741 agccgttccg cctcgtgcgg gccgggcgcg acgagcagca gcggagcgcc gagcgcccga
     1801 gcgagcgccg ccaccagcgc gtcctcgccg gcgtccgctc gggcgacgag cgggcgggcg
     1861 cgctcgagca ggaggcgttc gaacgcctcc gacccgatca gcgcgtcgag cgcctgctcc
     1921 aggctcacct gatctccttc gactcggccc ttggctcgtg ctttggcacc gtcaccggac
     1981 agcacgaaca ggcctcgttc ggcgaacgag gcctttccac gcgatcgtct tcgcgcccca
     2041 ctacggttcg cgcggagcgc gattgtatcg gtcctgcgtc ggaagcaggc cgtcggcgat
     2101 cagggaccgg accgcgtcgg ccgcatcgtc gaccagcaga gccacgtcgg gctcctcgcg
     2161 cttggcgaac gcgtcgagca cccagtccgc cgcttgcttt cgaccggtcg gacggccgac
     2221 tccgaggcgc acgcggaaga agtcgggcgt tcggagcgcc tgctgcagcg aacgcaggcc
     2281 gttgtgaccg gccgtcgagc cgccggactt gacgcggagc gccccgaagg gcagatcgat
     2341 ctcgtcgtgc accgcgatca cgtggtcggg ttcgacgccg tgcttgcgcg cgagcgaggc
     2401 gatcgccgga ccgctctcgt tcatgaacgc gcgcgacttc gcgagcacga cgcgttcgcc
     2461 ccccacgcgg atctcgctca cgtccgcgcc gacgaagcgg gccttgcgga accgttcccg
     2521 ctcgcttgca acgagctcgt ccacgacccg gtagccgagg ttgtggcggg tcctcgcgta
     2581 gcgctcgccg gggttgccga gccccgcgac gagccaggtc atgtcggcga gggcgtctag
     2641 ccctcggacc gctcgtcgga gccctcggcc ccctcgcctt gggcggcctc gccctcctcg
     2701 ccctcggcgg cctcgccctc cgcggcctcg gcctcggcgg cggcggcctc ctcctcctcg
     2761 agctcgacgg ccatctgggg ctgctgcacc gcgacgacgg actcctcggg gttggtcagg
     2821 atcgtcacgc ccttgggtgg gacgagatcg ctcacgcgca gcgacatccc gatctccagg
     2881 gacgtgatgt cggcgtcgat cgcctcgggc acgtcgcccg gcagggactc gacctcgagc
     2941 tcccacaggt ggtgctcgac gacgccgccg gccttcacgc cgacggattc gcccacgatg
     3001 cgcaccggga tcgagaggtg gagcttctcg tcacggcgga cggcgaggaa gtcgatgtgg
     3061 atgaagcgac cgtggatgtg gtcgcgctga acgtcgcgag ggatgatcag gtgctcggtc
     3121 ccgtcgacga cgaggtcgac caggacgttg gtgccggcgc cggtatggag gacgtggaac
     3181 aggtccttgg cgtccacgga cagcggggtc gagtccatcc cgtgcccgta gagcaccccc
     3241 ggcacgcggc cggcggcccg cagcttgcgg gcgacgccct t
//
LOCUS       DATBRK010000091         6087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_590488, whole genome shotgun sequence.
ACCESSION   DATBRK010000091 DATBRK010000000
VERSION     DATBRK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590488"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..330)
                     /gene="fdxA"
                     /locus_tag="VIH70_02705"
     CDS             complement(1..330)
                     /gene="fdxA"
                     /locus_tag="VIH70_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005478968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY8115303.1"
                     /translation="MAYVIAEPCIDIKDKACVDECPVDCIYEGPRMLYIQPDECVDCD
                     ACVPVCPVTAIFSDEDLPGEWKQFTAVNAEWFSDAVTGLGSPAGAAQVGPQDKDHPVV
                     AGWSAVA"
     gene            411..1658
                     /locus_tag="VIH70_02710"
     CDS             411..1658
                     /locus_tag="VIH70_02710"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY8115304.1"
                     /translation="MPIVVQKYGGTSVGDTDRIRGVADRIVRAREQGQDVVVVVSAMG
                     ETTDDLLDKASDITPAPEPRELDMLLTAGERIAMSLLAIAVNARGCRAASYTGSQAGI
                     ITDTQHGKARIVEIRPTRIKESLDAGNVVILAGFQGLSTEYDITTLGRGGSDTTAVAM
                     AAALGAEVCEIYTDVDGVFTADPRTHPEARRIDRISYEEMLELAAAGARVLQLRCVEY
                     ARRHGVRIHVRNSLGEQPGTWVDEDEEASTGMLESALIRGVALDVEEAKVTLDEVPDR
                     PGIAAAIFKAVAAEGIHVDMIVQNVSHDGRTDLSFTIPRADLHRLDTVLEGIVTDVGA
                     LRYETDAAIAKLSLVGAGIKSNPGVAADMFDALAAEGINIDMISTSSIRISCVIRAED
                     GERAVRAVHQRFGLDGATLGEEA"
     gene            1661..2347
                     /locus_tag="VIH70_02715"
     CDS             1661..2347
                     /locus_tag="VIH70_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115305.1"
                     /translation="MVVCPTCREVNEEGRAICQKCGSSLTPDAVALLPRREPGERAPI
                     EMRKPPQPSRWRPLIVLGLLLGGAMAVGAFLVLRPDPCEDTNFESENFGYCLLVPSGW
                     EAGPAQFGADVTLDQFAPPTGSATVVVEAVDLETGVELDQWSEFVRQRDEDEGLTPGP
                     ASETRLDGADALQWDVSVASEGGDTFLMREVVTVSNDVGWRVTLNDLQEGFDTSAVVF
                     RDMLDSWQFA"
     gene            complement(2376..2951)
                     /locus_tag="VIH70_02720"
     CDS             complement(2376..2951)
                     /locus_tag="VIH70_02720"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8115306.1"
                     /translation="MTDDTAPSPEITLSYRGEVVRLAAIDPEADAETFAAWFDDPVST
                     RLAGFRPVWPMNRASAQERLEEWVKASPGAINFAARTIADDRLVGGIGLMDINHPDGN
                     AQLGLSVYRQDDWGRGYGGEMIVLALRYAFNELNLHRVWLTTSDFNERALKLYEKIGF
                     RHEGRGREHILLDGTRWDLVYMGILRDEFLG"
     gene            complement(2995..3258)
                     /locus_tag="VIH70_02725"
     CDS             complement(2995..3258)
                     /locus_tag="VIH70_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115307.1"
                     /translation="MGEMDMVDELETKASHHHEATVHDHKHFHVTHYLHRGENWGHLL
                     STHVHEHNHPELEHVHMPHEDIEKEHRREGHIHDHARPADSPA"
     gene            3333..4367
                     /locus_tag="VIH70_02730"
     CDS             3333..4367
                     /locus_tag="VIH70_02730"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY8115308.1"
                     /translation="MSGVRVAIVGATGAVGRTMLEILEERDFPLDELTLLASERTAGT
                     RLRFRDREHTVALLTPEALDGVDLALSSCGSAIAETWVPQAAEAGATVVDNSSAFRME
                     PWAELVIPEVNPEALERGARVYAVPNCTIITALMALAPLHRVAGLRAVSLSSYQSVSG
                     AGAKGVRELAEQVEKLHGMEDDLAHPDQDALPVGDVVGKTIAYNVVAKIDVFDEQSGF
                     TFEEIKVQREARRILSLPELDISATCVRVPVAVGHSVSLHATFERPLSPGEAREVLEA
                     AEGVQVRDDPANEIYPSPLEAAGVDDVLVGRIRRPEGHDDELMLFACADNLRKGAALN
                     AVQIAERVTA"
     gene            4595..>6087
                     /locus_tag="VIH70_02735"
     CDS             4595..>6087
                     /locus_tag="VIH70_02735"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY8115309.1"
                     /translation="MRSRIGITSLALAAVFVLFMATPTTAGPFTVSPLTVVSGPSPFA
                     GCDLSGQPGTNFLNSEVEPWVEVNPTNPNNVIAVWQQDRWSNGGARGLLTGVSHDGGQ
                     SWTTTFPHFSTCAGGTAANGGNYERASDPWVTFAPNGDAYQISLSVNFVNDPATAILV
                     SKSTDDGDNWTEPITLIRDPSGVAPFLFNDKESITADPTDANRVYAVWDRTRFPSDNA
                     NFNAQHAFSFRGDIVFARTLDAGDTWEPAHTIFEPRKNQATIGHQIVVQPDGDLFDFF
                     TFFQGSGNNAPGSSLAYVTSTDHGATWSDPIVIRKTRFIGAFDPDTGLPIRAEGFTPE
                     VAVDRSNGNLYVTWQDTMFAGVDQVAFTMSSDDGATWSDPVKVSQTPSSSDVANEQAW
                     VQAVAVADDGTIAVTYYDFRNNDQETGAPTDHWLVHCHAACDDADSWDDEVRLTGSSF
                     DIEQAPFARGPNGFFLGEYEGLSNIGDAFVPLFAIVNDGNTANRTDIV"
BASE COUNT          954 a         2120 c         2081 g          932 t
ORIGIN      
        1 tcaggcgacg gccgaccacc cggcgacgac cgggtggtcc ttgtcctgcg ggccgacctg
       61 ggccgctccg gcgggcgatc ccagcccggt cacggcgtcc gagaaccact cggcgttgac
      121 cgccgtgaac tgcttccact cgccggggag gtcctcgtcc gagaagatcg cggtgacggg
      181 gcagaccggc acgcaggcgt cgcagtccac gcactcgtcc ggctggatgt acagcatccg
      241 agggccctcg tagatgcagt cgaccgggca ctcgtcgacg cacgccttgt ccttgatgtc
      301 gatgcacggt tccgcgatga cgtaggccat tggtgtccct ttcccgggcg ccggctggat
      361 gaaactgtac cccacaccga cccaacggac cccgctgcta ccctcctcgc atgcccatcg
      421 tggtgcagaa atacggcggg acctccgtcg gcgacaccga ccgcatccgg ggcgtcgcgg
      481 accggatcgt ccgcgcgcgc gagcagggcc aggacgtcgt cgtcgtggtc tcggccatgg
      541 gcgagaccac ggacgacctc ctcgacaagg cctcggacat cacgccggcc cccgagccgc
      601 gcgagctcga catgctcctc acggccggcg aacggatcgc catgtcgctc ctcgcgatcg
      661 ccgtcaatgc ccgtgggtgc cgagccgcct cgtacacggg ctcgcaggcc gggatcatca
      721 ccgacaccca gcacggcaag gcccgcatcg tcgagatccg ccccacccgg atcaaggagt
      781 ccctcgacgc cggcaacgtc gtgatcctgg ccgggttcca gggcctgtcg accgagtacg
      841 acatcacgac gctcggacgc gggggctcgg acacgaccgc cgtggcgatg gcggcggccc
      901 tcggggcgga ggtgtgcgag atctacaccg acgtcgatgg cgtcttcacc gccgatcccc
      961 ggacccaccc cgaggcccgc aggatcgacc gcatctccta cgaggagatg ctcgagctcg
     1021 ccgccgccgg cgcgagggtc ctccagctgc ggtgcgtgga gtacgctcga cggcacggcg
     1081 tccggatcca cgttcggaac tccctgggtg agcagccggg cacgtgggtc gacgaagacg
     1141 aggaggcttc aaccgggatg ctcgagagcg ccttgatccg cggcgtcgcc ctggacgtcg
     1201 aagaggccaa ggtcaccctc gatgaggtac cagaccggcc cggcatcgcg gccgcgatct
     1261 tcaaggcggt cgccgccgag ggcatccacg tcgacatgat cgtgcagaac gtctcgcacg
     1321 acgggcgcac cgacctgagc ttcacgatcc cgcgcgccga tctgcaccgc ctcgacacgg
     1381 tgctcgaggg catcgtgacc gacgtcggcg ccctccgcta cgagaccgac gccgcgatcg
     1441 ccaagctctc gctcgtcggc gccgggatca agagcaaccc cggcgtggcc gcggacatgt
     1501 tcgacgcgct cgcggccgag ggcatcaaca tcgacatgat ctcgacctcg tcgatccgca
     1561 tctcctgcgt gatccgggcc gaggacggcg agcgcgccgt ccgcgccgtc caccagcggt
     1621 tcggtctgga cggcgcgacg ctcggcgagg aggcctgagc gtggtcgtct gcccgacctg
     1681 ccgcgaggtg aacgaggagg ggcgcgcgat ctgccagaag tgcggctcct cgctcacccc
     1741 cgacgccgtc gccctcctcc cgcgtcgcga gcccggcgag cgcgcaccga tcgagatgcg
     1801 caagcctccc cagccctcga gatggcggcc gctgatcgtg ctcggcctgc tcctcggcgg
     1861 ggccatggcc gtcggcgcgt tcctcgtgtt gcggcccgac ccgtgcgagg acacgaactt
     1921 cgagtcggag aatttcggct actgcctgct ggtccccagc ggctgggagg cgggtccggc
     1981 gcagttcggc gccgacgtga cgctcgacca gttcgctccg cccacgggtt cggcgaccgt
     2041 ggtcgtcgag gccgtcgacc tggagaccgg ggtcgagctc gaccaatggt cggagttcgt
     2101 ccgtcagcgc gatgaggacg aggggctcac gccggggccg gcgagcgaga cgaggctgga
     2161 cggcgccgac gcgttgcagt gggacgtctc tgtcgcgtcc gagggcggcg acacgttcct
     2221 catgcgggag gtcgtcacgg tgagcaacga cgtcgggtgg cgggtcacgc tgaacgatct
     2281 ccaggaaggg ttcgacacga gcgcggtcgt gttccgggac atgctcgact cctggcagtt
     2341 cgcgtaggcg ggctcgcggc gctcagcccc agcgatcagc cgaggaattc gtcccgcagg
     2401 atccccatgt agacgaggtc ccaccgcgtg ccgtcgagca ggatgtgctc gcgaccgcgt
     2461 ccctcgtggc ggaacccgat cttctcgtac agcttgagcg cgcgctcgtt gaagtcgctc
     2521 gtcgtgagcc acactcggtg caggttgagc tcgttgaacg cgtagcggag cgcgaggacg
     2581 atcatctccc cgccgtagcc acgaccccaa tcgtcctgcc ggtagaccga caggccgagc
     2641 tgcgcgttcc cgtcggggtg gttgatgtcc atgagcccga tgccgccgac gaggcggtcg
     2701 tcggcgatcg tacgcgcggc gaagttgatc gcgcccggtg acgccttcac ccactcctcg
     2761 agcctttcct gcgcgctcgc ccgattcatc ggccacaccg gccggaaccc cgccagccgc
     2821 gtgctcaccg ggtcgtcgaa ccacgcggcg aacgtctccg cgtccgcctc cgggtcgatc
     2881 gcggccagcc ggacgacctc gccccgatag ctgagcgtga tctccggcga gggcgccgtg
     2941 tcgtcggtca tcgtccgagc atgctacgag gtgggcgccg gcaagagacg gaggtcaggc
     3001 gggggagtcg gccggccggg cgtgatcgtg gatgtgcccc tcgcgccggt gctccttctc
     3061 gatgtcctcg tgcggcatgt gcacgtgctc cagctcggga tggttgtgct cgtgcacgtg
     3121 ggtggacagt aggtgccccc agttctcgcc ccgatgcagg tagtgggtca cgtggaagtg
     3181 cttgtggtcg tgcaccgtgg cctcgtgatg gtgactggcc ttcgtctcga gctcgtcgac
     3241 catgtccatc tctcccatcg cagcctcctc aggcactcga cgcttcgaag gtacccccgg
     3301 cgggccgggt cacaccaggt cccaccggta ccatgagcgg tgtgcgtgtc gcgatcgtgg
     3361 gtgcaaccgg ggcggtcggc cggacgatgc tcgagatcct cgaggagcgc gacttcccgc
     3421 tggacgagct gaccctgctc gcctcggagc gcacggcggg cacccggctc cggttccggg
     3481 accgggagca cacggtcgcc ctgctcacgc ccgaggcgct cgatggcgtg gacctggcgc
     3541 tgtcgagctg cgggagcgcg atcgccgaga cctgggtgcc gcaggccgcc gaggccggcg
     3601 ccaccgtggt cgacaactcg agcgcgttcc gcatggagcc gtgggccgag ctcgtgatcc
     3661 ccgaggtgaa tcccgaggcg ctcgaacgcg gcgcgagggt ctacgcggtg ccgaactgca
     3721 cgatcatcac cgcgctgatg gcgctcgcgc cgttgcaccg ggtcgccggc ctcagggccg
     3781 tgagcctgtc ctcgtaccag tcggtctcgg gcgccggcgc gaagggcgtg cgagagctgg
     3841 ccgagcaggt cgagaagctc cacgggatgg aggacgacct cgcgcacccc gatcaagatg
     3901 cgctgccggt cggcgacgtg gtcggcaaga cgatcgcata caacgtggtg gccaagatcg
     3961 acgtgttcga cgagcagagc gggtttacgt tcgaggagat caaggtgcag cgggaggcca
     4021 ggcggatcct gtcgctgccc gagctcgaca tctcggccac gtgcgtgcgg gtgccggtgg
     4081 ccgtcggcca cagcgtgagc ctgcacgcga cgttcgagcg gccgctgtcg cccggcgagg
     4141 cgcgcgaggt cctcgaggcg gccgagggcg tccaggtccg cgacgatccg gccaacgaga
     4201 tctacccgag ccccctcgag gccgccgggg tcgacgacgt gctcgtcggc cggatccgcc
     4261 ggcccgaggg ccacgacgac gagctcatgc tgttcgcgtg cgccgacaac ctccgcaagg
     4321 gcgcggccct gaacgcggtg cagatcgccg agcgcgtgac cgcctgagcg ccggctctgg
     4381 cggccgcccg acgaaggatc gtcggcccgc accgtcagcg ttctgtctgt cagggatctg
     4441 acccgtccgg gttcagactg acagaaccct gtcgccgggg cgacgcacgg gcaatgcctt
     4501 gcgagtgcgg aggcgaccgt tgtacggtcg tttcaaggac gccggtacgc tcggtcgggc
     4561 tcgtgggggg atccgggcag gcaaggaggc ccccatgcgt tcgaggatcg ggatcacgtc
     4621 gttggctctc gcggccgtct tcgttctgtt catggcgaca ccgacgaccg cgggaccctt
     4681 caccgtgtca ccgctcacgg tcgtgtccgg accgagtccg ttcgccggct gcgacctctc
     4741 tgggcagccc gggaccaact tcctcaacag cgaggtcgag ccctgggtcg aggtcaaccc
     4801 cacgaacccg aacaacgtca tcgccgtgtg gcaacaggac cgctggtcaa acggcggggc
     4861 tcggggccta ctcacgggtg tgtcccacga cggggggcag tcctggacca ccaccttccc
     4921 gcacttcagc acctgcgccg gcgggacggc cgccaacggg gggaactacg aacgagcctc
     4981 ggacccctgg gtgaccttcg cgccgaacgg cgatgcctac cagatcagcc tgtccgtgaa
     5041 cttcgtgaac gacccggcca cggcgatcct ggtgagcaag tccacagacg acggcgacaa
     5101 ctggacggag ccgatcacgc ttatccgcga ccccagcggc gtcgcgccct tcctgttcaa
     5161 cgacaaggag tcgatcaccg ccgatcccac cgacgccaac cgcgtctacg ccgtgtggga
     5221 tcgcacccgg ttccccagcg ataatgcgaa cttcaacgct cagcatgcgt tctcgttccg
     5281 gggcgacatc gtcttcgctc gcaccctgga tgccggcgac acgtgggaac ccgcgcacac
     5341 gatcttcgag ccgaggaaga accaggccac gatcggtcac cagatcgtcg tgcagcccga
     5401 cggggacctg ttcgacttct tcacgttctt ccagggctcg ggcaacaacg caccgggctc
     5461 cagcctcgcc tacgtcacat ccacggatca cggcgcgacc tggtcggatc cgatcgtgat
     5521 ccgcaagacc cggttcatcg gcgcgttcga ccccgacacg ggcctgccga tccgggccga
     5581 aggcttcacc cccgaggtcg cggtcgaccg gtcgaacggc aacctctacg tgacctggca
     5641 ggacacgatg ttcgcggggg tggatcaggt cgcgttcacg atgtcgagcg acgatggtgc
     5701 tacctggtcg gaccccgtga aggtgagcca gacgccgtcg agctccgacg tggcgaacga
     5761 gcaggcatgg gtgcaggccg tggccgtggc ggatgacgga acgatcgccg tcacctacta
     5821 cgacttccga aacaacgacc aggagacggg agcccccacc gaccactggt tggtgcactg
     5881 ccacgccgcg tgtgacgacg cggacagttg ggacgacgag gtccggctga ccggcagctc
     5941 gttcgacatc gagcaggcgc cgttcgcccg aggcccgaac ggcttcttcc tcggggagta
     6001 cgagggactg tcgaatatcg gtgacgcctt cgtgccgttg ttcgccatcg tgaacgacgg
     6061 aaacacggcc aaccggaccg acatcgt
//
LOCUS       DATBRK010000092         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_298006, whole genome shotgun sequence.
ACCESSION   DATBRK010000092 DATBRK010000000
VERSION     DATBRK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298006"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VIH70_02740"
     CDS             <1..720
                     /locus_tag="VIH70_02740"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY8115310.1"
                     /translation="KLASRDAKPDGMVVVRADEVDAYLEPLPVRALWGVGEKTAETLG
                     RLGVRTVGDLGRTPVGVLGRLMGERHARDLRDLARGEDDRTVVPYEAPKSVSHEETFD
                     RDLDDRRELLREVLSLSQRVAARLREDGFRTRTVTLKIRLANFTTLTRSRTLTAPTDV
                     AADLYEVVGELFTALPGARRRVRLLGVVATGLVPAGEQQLALLRSERWGDVERAVDRI
                     DRRFGPGSAVPAALLERGRDR"
     gene            825..1214
                     /locus_tag="VIH70_02745"
     CDS             825..1214
                     /locus_tag="VIH70_02745"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEY8115311.1"
                     /translation="MPLSPDEQRILDEIEQRLAEEDPRLVEAVSRTSLYTHLARRIKW
                     SLLAFLVGFLMLMVFLMVVPDLLWVAATGFGVMLAAALLIYRYLKQLTLDQMRAMQQQ
                     SGRFSLTGLLARLAGRFRTPPATPPGS"
     gene            1480..>1787
                     /locus_tag="VIH70_02750"
     CDS             1480..>1787
                     /locus_tag="VIH70_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8115312.1"
                     /translation="MEGFTAHQATKFTGCSARQLRYWDQIGLVNPSIQGTGGRPGVPR
                     LYSFRDLVALKVVKSLLDNGMSLQRVRRAWDYLNRRAALDKHLSEVKLITDGVSIFKI
                     A"
BASE COUNT          261 a          600 c          626 g          300 t
ORIGIN      
        1 aagctcgcct cgcgcgacgc gaagcccgat gggatggtcg tcgtgcgcgc cgacgaggtg
       61 gacgcctacc tcgaaccgct gcccgtgcgg gcgctctggg gggtgggcga gaagacggcc
      121 gagaccctcg ggcggctcgg cgtgcggacc gtcggcgatc tgggccggac cccggtcggg
      181 gtgctcggca ggctcatggg cgaacggcac gcgcgcgacc tcagggacct tgcccgtggg
      241 gaggacgacc ggacggtcgt gccctacgag gcgccgaagt cggtcagcca cgaggagacc
      301 ttcgaccgcg acctggacga tcggcgcgag ctgctccgcg aggtgctctc gctctcccag
      361 cgggtggccg cccggctgcg cgaggacggg ttccggaccc ggacggtcac cctgaagatc
      421 cggctcgcga acttcacgac gctcacgcgt tcgcgaacgc tcacggcgcc gaccgacgtc
      481 gccgcggacc tgtacgaggt ggtcggggag ctgttcaccg ctttgccggg ggcccgccgg
      541 cgcgtccgat tgctcggagt ggtcgcaacg ggcctggtgc cggcggggga gcagcagctc
      601 gcgctgctgc ggagcgagcg gtggggcgac gtcgaacgcg cggtggatcg gatcgatcgg
      661 cggttcggac cgggatcggc cgtgcctgcg gccctgctcg aacgcggccg cgatcgctga
      721 acgtccgccc ggacgcgccc ggcctccgtc gaaggcccct tccactggcg gaggtcgttc
      781 ctataatcgt gtcggaccgt catcgaggcc ggagccggga gcctatgcca ctgtcgcccg
      841 acgaacagcg catcctcgac gagatcgagc aacgtctcgc cgaggaggat ccccggctgg
      901 tcgaggccgt cagccgaacc tccctgtaca cgcatctcgc ccggcgcatc aagtggtcgc
      961 tgctggcgtt cctggtcggc ttcctgatgc tgatggtgtt cctgatggtc gtccccgacc
     1021 tgctgtgggt cgcggccacg ggcttcggcg tgatgctcgc ggccgcgttg ctgatctatc
     1081 gatacctcaa gcagctgacc ctcgatcaaa tgcgtgccat gcagcagcag tcggggcgtt
     1141 tctcgctcac cggcttgctg gcgcgtctcg ccggacgttt ccgaacgccg cctgcgacac
     1201 cccccggttc ctaagcgttc ggtcgagcgc gcggttccct cggtcgtctc gtcgccgatc
     1261 gacgggcatg acgagcccgt tgcgatcgaa tgtgagaaac atccgagaga cccgccatgt
     1321 cgtcgtcctc gggttgccac ggcacacgcg cctggagtac aacttgcctc accttccccg
     1381 ggggcgtgac cgtcgatccg acgtcgagac ttcccctcga cgagccggcg gaacgcgttg
     1441 gtgtcgtgag ggagacgtcg tcggagggcg ctcgttccta tggagggatt taccgcacat
     1501 caggcgacga agttcaccgg gtgctccgcg cgtcagctca ggtattggga ccagatcggc
     1561 ttggtcaacc cgtccatcca ggggaccgga ggccgtccgg gggtcccaag gttgtactcg
     1621 ttccgggacc tcgtcgcgct caaggtcgtc aaatccctgc tcgacaacgg gatgtcgctc
     1681 caacgtgtgc gccgggcatg ggactacctg aatcggcggg ccgcgctgga caagcacctg
     1741 tccgaggtca agctgatcac cgacggggtc agcatcttca agatcgc
//
LOCUS       DATBRK010000093         7427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1310944, whole genome shotgun sequence.
ACCESSION   DATBRK010000093 DATBRK010000000
VERSION     DATBRK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310944"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VIH70_02755"
     CDS             complement(<1..292)
                     /locus_tag="VIH70_02755"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEY8115313.1"
                     /translation="MTEPLVGVVMGSASDWDTMQHCAKQLEELRVPFETRVLSAHRTP
                     KELAAWIEDAESRGVEVFIAAAGMAAALPGVVAAHTTRPVLGVPMESKLAGGL"
     gene            complement(351..1763)
                     /locus_tag="VIH70_02760"
     CDS             complement(351..1763)
                     /locus_tag="VIH70_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8115314.1"
                     /translation="MTEADVPLRLDAPREEVLEHAAKLVAEAWQSFDRYRPEEPPLDE
                     RVRRLLRTELPTTPGAAHEAVDDAARILDESIAQPRPRYFVGVGSSGLEIGTIGDLLA
                     HTYDINLAVDARAATLIERQAVRWAAEFIGYAPDAAGAFTSGGTVSNTTALAAAREHA
                     LPGSRYEGLGGKRVAIYCSQEVHYSVTRAVETLGVGSANLRAIPLGAERGLEPDALAV
                     AIDRDLADGVTPIAVVATAGTTLTGAIDPLEAIADICEERGVWFHVDGAYGLPAVAAR
                     PELFAGFERAQSVAVDAHKWLYLPKACGILLAREQEDLVASFGHEQGYLPHQRHELHA
                     METTFEYSRPFRALKFWLAMRVHGAAAFAEAIERNLQQAQLLYRTVSERDDFEALAPP
                     KLSILAFRHAPPGVEDVGAHNQRLAEAIQADGRVWMSSALIDEEVWLRPCFVNFRTTE
                     EDVLDVVEIAAEIGERIATG"
     gene            complement(1760..2701)
                     /locus_tag="VIH70_02765"
     CDS             complement(1760..2701)
                     /locus_tag="VIH70_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8115315.1"
                     /translation="MADAGERTSRPDRTTLAAFATFVTLAGGNVIAVRSISCEDCELD
                     PFWAAASRFLLASLILVAIALVVGGGMPRGRALLGAALYGVLGFGAAFAFAYWGLQRV
                     SAGLGAVFLATVPLLTFAFALLHRQERFKWDGLIGGVFAVAGMAVIFREGLGAGMPLG
                     SLLSLFAAAVCFAETGIVVKAFPRVHPISMNAIGLGVGGLMLLVLAWLHGDSLAVPEL
                     AKTWVAQAYLVLLGSVVAFSLYVYVLRRWTASAVSYEGVLIPIVAILLASWLQDEEIT
                     WAFATGSVLVLVGVYVGALRRPAGVPDLEPGRVASEP"
     gene            complement(2733..3872)
                     /locus_tag="VIH70_02770"
     CDS             complement(2733..3872)
                     /locus_tag="VIH70_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY8115316.1"
                     /translation="MRSARHPARAEVVGSLLRPSALRKAIDDFYERGHSAVLAEERDK
                     DRTALREVEDDAIRVAVQRQIDLGLDVVTDGEFRRWMFLNSFYDAVEGFRTDNVVTFR
                     NAQGEKVPLRVHEIVERLRPVDSPAAREADFVSTVADGYPFKITFPAPSLFGHPFSYK
                     PGVTSGYGSLEEFVGHAIQIERGLVADVIKAGARYVQFDFPLYPYLVDPAWVERFEAQ
                     GHRVSDLVDEAIATDTAVLEGIPDDVTVALHICRGNYRSSWMCEGSLEPVAERVFGEL
                     PYDAFLVEWDDLGRDGGFESVRFLRKGSTMVMGIVSTKTPALEDDDDLIRKMEGAASL
                     AGGMDRLAISPQCGFASVMVGNEIDEDTQWRKLELVARVADRIWG"
     gene            complement(3970..5172)
                     /locus_tag="VIH70_02775"
     CDS             complement(3970..5172)
                     /locus_tag="VIH70_02775"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEY8115317.1"
                     /translation="MSERRKEVFMMIEEFGGGVGVRSWASILDDKTREQAEKTALLPI
                     LTEPIALMADAHLGYGATIGSVIPTESAVIPSAVGVDIGCGMAARKVDLAGTTVLELA
                     ESWVHNCEASIPAGMGKGRTEFSAAAKEWMSAHTPPATLANPRRAEAQLGSLGSGNHF
                     IELAVDQHDGTWILLHSGSRGPGNQLATLHTKVAMDLHERLGTALEDPELSWLQSNTP
                     EFDAYIDDLRWAQAFALENRRLLLDGAHHQLNEANGESVRTLDEVNCHHNYTELEEHH
                     GKWLWVTRKGAIRAGVGDRGLIPGAMGQKSFVVRGLGNPLSYESCSHGAGRVLSRSKA
                     RKTLSVADLESQMMGRSWQASEAKSLLDEAPQAYKPLDVVMRDQADLVEIEVELSSVA
                     NYKGVEQR"
     gene            complement(5469..6662)
                     /locus_tag="VIH70_02780"
     CDS             complement(5469..6662)
                     /locus_tag="VIH70_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8115318.1"
                     /translation="MGAGEARMTLGERIMLEELERDPYPIYARLREEEPVAWVPAVQL
                     WLVTRYDDVRTVDVSPEVFTAATDPSTLNRTMGVNMLGSEGPDQQRVRRVVEPPFRPR
                     DVEERTRGMIPALANELIDGFEGRGEADLFTEFADPMSVRSLRFMLGLDDVAWEDLLR
                     WNQGLMLGLANFEGDPAKQAPADEASASLSEVIERVLDHLEDEPDGSVLSAMLHHDRD
                     GDRITREEIVANTKLMLSGGLQEPRDVIALVIHALGSNPSQLEEVRSNPRLVKSAVEE
                     TLRWAGPVGTSTRQTTETTQLAGVKLEEGALIGAVLSSANRDARRFTDPDRFDIHRKE
                     GAHLAFAVGNHFCLGAWFGRHLARVSLEILLERLPGLRLDPERSAMLSGWEFRAPDST
                     WVRWG"
     gene            complement(6638..7216)
                     /locus_tag="VIH70_02785"
     CDS             complement(6638..7216)
                     /locus_tag="VIH70_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8115319.1"
                     /translation="MQPLRREDHPELEELFRLYDDTMSFVPNSLFTMARRPEILRAFS
                     ELITQIWRTGTVPVGLKPLVAIVASTAAGCRYCQAHETVDAKMRGVDEDKIAEIWDFE
                     RSPRYSEAERAALRFARDASVVPNAVTPAHFEDLRRYWDDGEIVEILAVVGLFGFLNR
                     WNDSMATDLEEIPTGFAGRTIGPHGWEPGKHG"
     gene            7291..>7427
                     /locus_tag="VIH70_02790"
     CDS             7291..>7427
                     /locus_tag="VIH70_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8115320.1"
                     /translation="MRLEGKVAIVSGGGTGIGAAAARLFAREGAKVVVTGRRAEPLEE
                     VA"
BASE COUNT         1202 a         2663 c         2440 g         1122 t
ORIGIN      
        1 cgagcccgcc cgcgagcttc gactccatcg ggacgccgag cacgggtcgc gtggtgtggg
       61 cggccacgac gccgggcagg gccgccgcca tgcccgcggc cgcgatgaag acctccacgc
      121 cgcgggactc ggcgtcctcg atccaggccg cgagctcctt gggcgtgcgg tgcgccgaga
      181 gcacccgggt ctcgaacggg acgcggagtt cctccaactg cttggcgcag tgctgcatcg
      241 tgtcccaatc ggacgccgat cccatcacga cgccgaccag cggctcggtc atcttgtcga
      301 gctccctctg tatgcgccgg cgcgagcagt ctagacgggc gggcggtccc tcatcccgtg
      361 gcgatccgtt cgccgatctc ggcggcgatc tcgaccacgt cgagcacgtc ctcctcggtc
      421 gtgcggaagt tcacgaagca cgggcggagc cagacctcct cgtcgatcag cgcgctcgac
      481 atccagacgc ggccgtcggc ctggatcgcc tcggccagcc gctggttgtg cgcgccgacg
      541 tcctcgacgc ccgggggggc gtgacggaac gccaggatcg agagcttcgg aggagcgagc
      601 gcctcgaagt cgtcccgctc cgacaccgtt cggtacagga gctgcgcctg ctgcagattg
      661 cgttcgatcg cctcggcgaa cgccgcggct ccgtgcacgc gcatcgcgag ccagaacttg
      721 agcgcccgga acggccgcga gtactcgaac gtcgtctcca tcgcgtgcag ctcgtgccgc
      781 tgatgcggca gatagccctg ctcgtgcccg aacgacgcga ccaggtcctc ctgctcgcgc
      841 gcgagcagaa tcccgcaggc cttcggcagg tacagccact tgtgcgcgtc gacggcgacg
      901 gactgcgcgc gctcgaaccc cgcgaacagc tcggggcggg cggccaccgc cgggagcccg
      961 tacgcgccgt ccacgtggaa ccacacgccg cgctcctcgc agatgtccgc gatcgcctcg
     1021 agcggatcga tcgcgccggt gagcgtggtg cccgcggtcg cgaccaccgc gatcggcgtc
     1081 acgccgtccg cgagatcgcg gtcgatcgca acggcaagcg cgtcgggttc gaggccccgc
     1141 tcggcgccga gcgggatcgc ccgcaggttc gccgagccga cgccgagcgt ctcgacggcc
     1201 cgggtgaccg agtagtgcac ctcctgcgag cagtagatcg ccacgcgctt gcccccgagc
     1261 ccctcgtacc gcgagccggg cagggcgtgc tcgcgcgccg cggcgagcgc ggtcgtgttc
     1321 gataccgtcc cgccgctcgt gaacgccccg gcggcgtcgg gcgcgtaccc gatgaactcg
     1381 gccgcccacc ggaccgcctg cctctcgatc agcgtcgcgg cgcgcgcgtc gaccgcgagg
     1441 ttgatgtcgt aggtgtgcgc gagcaggtcg ccgatcgtgc cgatctccag gccgctcgag
     1501 cccaccccga cgaaataccg ggggcgcggc tgcgcgatcg actcgtcgag gatccgtgcc
     1561 gcgtcgtcga cagcctcgtg agccgccccg ggcgtggtcg ggagctcggt ccgcaggagc
     1621 cggcggacgc gttcgtcgag cgggggctcc tcgggccggt accggtcgaa gctctgccac
     1681 gcctcggcga ccagcttggc ggcgtgctcg agcacctcct cgcgcggggc gtcgagccgc
     1741 agcgggacgt cggcctcggt cacggttcgg atgctacccg gcccggttcg agatccggca
     1801 cacccgcggg ccggcggagc gcgcccacgt agaccccgac cagcacgagc accgaaccgg
     1861 tcgcgaacgc ccaggtgatc tcctcgtcct ggagccacga cgccagcagg atcgccacga
     1921 tcgggatcag cacgccctcg tacgagaccg cgctcgccgt ccagcggcga aggacgtaga
     1981 cgtacagcga gaacgcgacg accgagccga gcaacaccag gtaggcctgc gcgacccacg
     2041 tcttcgcgag ctcgggcacg gccagggagt cgccgtggag ccacgcgagg acgagcagca
     2101 tcagcccgcc cacgcccaac ccgatcgcgt tcatcgagat ggggtggacg cgcggaaacg
     2161 ccttgaccac gatgccggtc tcggcgaagc acactgcggc cgcgaagagc gagagcagcg
     2221 agcccaatgg catgccggcg ccgagcccct cgcggaagat caccgccatg cctgccacgg
     2281 cgaacacgcc gccgatcagg ccgtcccact tgaaccgctc ctgccggtgg agcagcgcga
     2341 acgcgaacgt caggagcggc acggtcgcga ggaacacggc cccgaggccc gcggacaccc
     2401 gttgcagccc ccagtacgcg aacgcgaacg cggcgccgaa tcccagcacc ccgtagagcg
     2461 ccgcgccaag caaggctcga ccgcgaggca tcccgccgcc gacgacgagc gcgatcgcca
     2521 ccaggatcag cgacgccagc aggaagcgcg aggcggccgc ccagaagggg tcgagctcgc
     2581 agtcctcgca cgagatcgat cggaccgcga tcacgttgcc gcccgccagc gtcacgaacg
     2641 ttgcgaacgc ggccagggtg gtccggtcgg gtcgactcgt tcgctccccc gcgtcggcca
     2701 tgaccgcaga tcgtagcccg atggactggc agtcatcccc agatccgatc ggccacccgg
     2761 gcgacgagtt ccagcttgcg ccactgcgtg tcctcgtcga tctcgttgcc gaccatcacg
     2821 cttgcgaacc cgcactgcgg gctgatcgcg agccggtcca tgccgcccgc gaggctcgcc
     2881 gccccctcca tctttcggat gagatcgtcg tcgtcctcga gcgccggcgt cttcgtcgag
     2941 acgatgccca tgaccatcgt ggaacccttg cggaggaagc gcaccgactc gaacccgccg
     3001 tcgcggccga ggtcgtccca ttcgaccagg aacgcgtcgt acgggagctc gccgaacacg
     3061 cgctcggcca ccggctcgag cgagccctcg cacatccacg aggagcggta gttccctcgg
     3121 cagatgtgga gcgccacggt cacgtcgtcg gggatgccct cgagcaccgc agtgtcggtc
     3181 gcaatcgcct catcgaccag atcggagacc cgatgaccct gcgcctcgaa gcgctcgacc
     3241 cacgccggat cgacgaggta cggatacagc gggaagtcga actggacgta tcgcgcaccg
     3301 gccttgatca cgtcggcgac gagcccgcgt tcgatctgga tcgcgtgccc gacgaactcc
     3361 tcgagcgagc cgtagcccga ggtcacgccg ggcttgtacg aaaatgggtg cccgaacagc
     3421 gacggcgccg gaaacgtgat cttgaacggg tacccgtcgg cgacggtgct cacgaagtcg
     3481 gcctcccgtg cggcgggcga gtcgacgggg cggagacgtt cgacaatctc gtggacgcgc
     3541 agggggacct tctccccctg ggcgttgcgg aaggtgacga cgttgtcggt gcgaaagccc
     3601 tcgaccgcgt cgtagaacga gttcaggaac atccatcgcc ggaactcgcc gtcggtgacc
     3661 acatcgagcc cgaggtcgat ctggcgctga accgcgactc ggatcgcgtc gtcctcgacc
     3721 tcacggaggg ctgtccgatc cttgtcccgc tcctccgcta acaccgccga atgcccgcgt
     3781 tcgtagaagt cgtcgatggc cttgcggagt gcagacggcc gcagcagcga gccgacaacc
     3841 tcggcgcgag cggggtggcg tgctgagcgc atccgatccg aagactagtg gacccagatc
     3901 ttccacagac ctacaggggt taactctgat cgtggaaacc gcgatctcga caagggttga
     3961 caacatctct catctctgtt cgaccccctt gtagttggca accgagctga gctcgacctc
     4021 gatctccacc agatcagcct gatcccgcat cacgacgtcc agcggtttgt acgcctgggg
     4081 cgcctcgtcg agaagcgact tcgcctcgga cgcctgccac gaccttccca tcatctgcga
     4141 ctcgaggtcc gcgaccgaca gcgtcttcct ggccttcgac ctggagagaa cccggcccgc
     4201 cccgtgcgaa caggattcgt acgacagggg gttaccgagg ccccgaacga cgaacgactt
     4261 ctgccccatc gcgcctggga tgagcccccg gtcgcccacc ccggctctga tcgctccctt
     4321 cctcgtcacc cagagccact ttccatggtg ctcctcgagt tcggtgtagt tgtggtggca
     4381 gttgacctcg tccagcgtcc gaacactctc gccgttcgcc tcgttcagct ggtggtgggc
     4441 gccgtcgagc aagagccgtc tgttctccag ggcgaaggcc tgtgcccacc gcaggtcatc
     4501 gatgtaggcg tcgaattcag gcgtgttgct ctgcagccac gacaactccg ggtcctcgag
     4561 agcggtcccg agccgctcgt gcaggtccat cgctaccttg gtgtggagcg tcgccagctg
     4621 gttgcccggg cccctggatc cggaatggag caggatccag gtcccgtcgt gctgatcgac
     4681 cgccagctcg atgaaatggt tgcccgatcc cagcgacccc agctgggcct ccgccctccg
     4741 cgggttcgcg agcgtcgccg gcggagtgtg agccgacatc cactccttcg cggcggcaga
     4801 gaactcggtc ctccccttcc ccatgccggc cgggatcgag gcttcgcagt tgtgcaccca
     4861 gctctcggcg agctcgagga ccgtcgtgcc ggcgagatcg accttcctgg ccgccatacc
     4921 gcatccgata tcgactccga cggctgaagg gatcaccgcc gactcggtcg ggattacgga
     4981 accgatggtt gccccatacc cgagatgagc gtcggccatc agcgcgatcg gctccgtgag
     5041 tatgggaagg agcgcggtct tttcggcctg ctctctcgtc ttgtcgtcga ggatggatgc
     5101 ccacgacctg accccgacac cgccaccgaa ctcctcgatc atcatgaaca cttccttccg
     5161 tcgctcagac acgccctcgg ttggacacct cgggaaatga agaacccctc cgtgggaggg
     5221 gtcaggcgca cgacggcaag aagcgacgcg cgcctaaggg gcgtcgcgtc gttgcaatgc
     5281 gttcgaggtg aacgtcttgc tcatggcgaa ggcgaagata caggaacgct atggcgaaga
     5341 caatgcctcc cggtcgctgg tgacggaagt gacatcctga gccatccgat ctcgcggcga
     5401 cggaagcttc gacagtagga caatcggatc gcagtcgatg aaaaacgaag ccgcaacccc
     5461 gaagaccgtc aaccccatcg aacccaggtc gagtccggag ctctgaactc ccatcccgac
     5521 agcatcgccg atcgctcggg gtcgagccga agtcccggca gccgctcgag caggatctcg
     5581 agcgagaccc gcgcgaggtg gcgcccgaac cacgccccga ggcagaaatg gttcccgacc
     5641 gcgaatgcca gatgcgcgcc ttccttccgg tggatgtcga accgatcggg gtcggtgaag
     5701 cggcgcgcgt cccggttcgc actcgagagc accgcgccga tcagcgcacc ctcctcgagc
     5761 ttcacccccg cgagctgggt cgtctccgtc gtttgccggg tcgaggtccc gaccggtccc
     5821 gcccaccgca gggtctcctc gaccgccgac ttgaccaacc gcgggttcga acgcacctcc
     5881 tcgagctgcg aagggttcga gccgagcgcg tggatcacga gcgcgatcac gtcgcgcggc
     5941 tcctggagcc cgcccgagag catcagcttg gtatttgcga cgatctcctc ccgcgtgatg
     6001 cggtctccgt cgcgatcgtg atgcagcatc gccgagagca cggaaccgtc gggctcgtcc
     6061 tcgaggtggt ccaggacgcg ttcgatcacc tccgagagcg acgcactcgc ctcgtcggcc
     6121 ggcgcctgct ttgccggatc gccctcgaag ttcgcgagcc cgagcatcag gccttggttc
     6181 cagcgcagga ggtcctccca cgccacgtcg tcgagcccga gcatgaatcg gagcgagcga
     6241 acgctcatcg ggtcggcgaa ctcggtgaac agatcggcct cgccccggcc ctcgaacccg
     6301 tcgatcagct cgtttgcgag cgccgggatc atgcccctcg tgcgctcctc gacgtcgcgc
     6361 ggccggaacg gcggctcgac gacccgacgg acccgctgct ggtcgggacc ctcggagccg
     6421 agcatgttga cgcccatcgt gcggttcagc gtcgaggggt cggtcgcggc cgtgaacacc
     6481 tcgggcgaca cgtcgaccgt ccgcacgtcg tcgtaccgcg tgacgagcca cagctgcacc
     6541 gcgggcaccc atgccaccgg ctcttcctcg cgcaggcgcg cgtagatcgg gtacgggtcg
     6601 cgctcgagct cctccaacat gatccgctcg cccaacgtca tccgtgcttc cccggctccc
     6661 acccgtgagg gccgatcgtc cggccggcga acccggtggg gatctcctcg aggtcggtgg
     6721 ccatcgagtc gttccagcgg ttcaggaacc cgaacagtcc gacgacggcc aggatctcga
     6781 cgatctcgcc gtcgtcccag tacctgcgga ggtcctcgaa gtgcgcgggc gtcacggcgt
     6841 tcgggacgac gctcgcgtcg cgcgcgaagc gtagggcggc gcgctcggcc tccgagtagc
     6901 gcggcgagcg ctcgaagtcc cagatctctg cgatcttgtc ctcgtcgacc ccgcgcatct
     6961 tcgcgtccac ggtctcgtgc gcctggcagt accggcagcc cgccgcggtg ctcgcgacga
     7021 tcgcgaccaa cggcttcagc cccaccggga ccgtcccggt ccgccagatc tgtgtgatca
     7081 gctcgctgaa cgcccggagg atctcgggcc ggcgggccat cgtgaacagg ctgttcggga
     7141 cgaaggacat cgtgtcgtcg tacagccgga agagctcctc cagctccggg tggtcctcac
     7201 gccgcagcgg ctgcatgttc gccacgcatc gacctccctg cgctcgtcgg cgccggtagg
     7261 gcagactatg ccccatcgcg aggaggcgag atgcggctcg agggcaaggt ggcgatcgtg
     7321 agcggcggcg ggaccgggat cggggcggcc gcggctcggc tgttcgcgcg cgagggcgcg
     7381 aaggtggtcg tcaccggccg ccgcgccgaa ccgctcgagg aggtcgc
//
LOCUS       DATBRK010000094         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2176948, whole genome shotgun sequence.
ACCESSION   DATBRK010000094 DATBRK010000000
VERSION     DATBRK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2176948"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1585)
                     /locus_tag="VIH70_02795"
     CDS             complement(32..1585)
                     /locus_tag="VIH70_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY8115321.1"
                     /translation="MRNDTYRRRWWILAVLAVSLLIIIIDDTIINVAIPTLQRELGAS
                     ASQLQWIIDAYIVVFAGLLLTMGSLGDRFGRKRFLQLGLVVFAGASVFGAYASSTSQL
                     IWARALMGVGGALIMPSTLSILIDVFPREERVKAIGIWTGVASLGIPIGPIVGGWLLE
                     RFWWGSVFLLNLPIVAVALSVGWFLLPESRDPDPPRPDVPGLVLSVAALSSLVYGIIE
                     APSSGWISVWVIGALTIAVVSGAALVVHEARTDQPMLDLRLFRNPRLAWGTAAVTLAG
                     LAIGGLAFELTQYLQFVQGYTPLQAGLRFLPLAIGFGIAGPISQRVVPRAGSGRTVAA
                     ALAVAAVFLVALSRVGPGTSYWIVAGVLLAIGLGIGAAFVPATDSVMAAVPGANAGFG
                     SALNDSGRQVGAALGIGILGAISNVLYASGIAGIAATLDPRAAEPVKASVGGALEVAS
                     GIGGSIGSGLREAAVASFMDGFRVAMLVGAALLGVGAVLVLRYLPSRDVEPQPDIVSQ
                     QELVPASQG"
BASE COUNT          359 a          657 c          614 g          247 t
ORIGIN      
        1 tccccgctgc ggcctccgtt ctgagcgtgc atcaaccctg gctcgccggc accaactctt
       61 gctgcgagac gatatccggt tgcggctcga cgtcccgcga tggcaggtaa cgcaggacca
      121 ggacggcacc gactccaagg agggcggctc cgacgagcat ggcgacgcgg aacccgtcca
      181 tgaatgaagc gaccgccgcc tcacggagcc ccgagccgat cgagccgccg atgccggatg
      241 cgacctcgag cgcaccgccg accgaggcct tgaccggctc ggccgcgcgg ggatccagtg
      301 tcgccgcgat acccgcgatc cccgacgcgt agagaacgtt ggagatcgcc ccgaggatcc
      361 cgatcccgag cgcggcgccg acctgtcgac cggagtcgtt gagcgccgag ccgaagccgg
      421 cgttggctcc tgggaccgcc gccatcaccg agtcggtcgc tggtacgaac gccgcaccga
      481 tccccagccc gatcgccaag agcacgcccg ccacgatcca gtaggaggtg ccgggaccga
      541 cgcgggagag cgcgactagg aagacggcag caaccgcgag ggcagcggcc accgtgcgcc
      601 ccgaacccgc ccgaggcacg acgcgctggc tgatcgggcc ggcgatcccg aagccgatcg
      661 cgagcggaag gaaccggagc cctgcctgca acggcgtgta gccctgcacg aactgcaggt
      721 actgggtgag ctcgaacgcg agcccaccga tcgcgagccc cgcgagcgtg accgccgccg
      781 tgccccaggc cagacgtggg ttccggaaca accgtaggtc gagcatcggc tggtccgtgc
      841 gtgcttcgtg caccacgagc gccgcgccgc tcacgacggc gatcgtgagg gccccgatca
      901 cccacacgct aatccagccg ctggacggcg cttcgatgat cccgtagacg agcgagctga
      961 gggcggcgac ggaaagcacg agtcctggca cgtccgggcg gggcggatcc ggatcccgac
     1021 tctcgggtag gaggaaccac ccgaccgaga gcgccaccgc aacgatgggg aggttcagca
     1081 ggaacacgga cccccaccag aaccgctcga gcagccaccc cccgacgatc ggtccgatcg
     1141 ggatgccgag gctcgcaacc cccgtccaga tcccgatcgc cttcacgcgc tcctcgcggg
     1201 ggaagacgtc gatcaggatc gagagcgtcg acggcatgat gagagcgcct ccgacgccca
     1261 tcagggcacg tgcccagatc agctgggacg tcgacgatgc gtaggcgccg aagacgctgg
     1321 cccccgcgaa caccaccagc cccagctgga ggaaccgctt gcgaccgaac cggtcgccca
     1381 gcgagcccat cgtcagcaac agacccgcga agaccacgat gtacgcgtcg atgatccact
     1441 ggagctggct ggcggacgcg ccgagctctc gctgcaacgt cgggatcgcg acgttgatga
     1501 tcgtgtcgtc gatgatgatg atcagcaggc tcacagcgag caccgccagg atccaccacc
     1561 ggcgccgata cgtgtcgttc cgcatcgttc cctcccctcc gttcgtgtcc gggctctggt
     1621 ttccggatgc tcgttcgtta ccgtcctcgc gtcctgtatc gaacatcgtg tgcgattatg
     1681 tggtacggtc ccggccgggc gtcaacaatc aaagtggccg cttccgttcg caaccgaacg
     1741 gaagcgcaga gatcgttcgg gaaaggacgc gagatggcgg agcgggtgaa gatggatcga
     1801 cgggtccgac gaacacgtga gctcctccga acagcgttaa ccacgctgat ccaagagcgc
     1861 ggatacgacc ggatcac
//
LOCUS       DATBRK010000095         6035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_876995, whole genome shotgun sequence.
ACCESSION   DATBRK010000095 DATBRK010000000
VERSION     DATBRK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876995"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VIH70_02800"
     CDS             complement(<1..372)
                     /locus_tag="VIH70_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8115322.1"
                     /translation="MGVFERDVVIIGGGHNGLACAAYLAKAGLDVLVLEKRGVVGGAA
                     ATEEPWPGYRVSSASYVVSLMPPQVVRELDLKRFGYEVSIITPDYFVPFPDGTSLTLW
                     GDVERDAAAIGRFSERDAAAYV"
     gene            522..1709
                     /locus_tag="VIH70_02805"
     CDS             522..1709
                     /locus_tag="VIH70_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115323.1"
                     /translation="MAVNSLQRRGLVNAAVAAVATFAIGSAVVYAAGGPDDVAAPPRS
                     SRPSASPKTPSCAPTWAVVRSANPGEAATTLQGVTAVTSAEAWAVGGLGEPEIPTAVA
                     IQRWDGDRWLAIAGPSPGSTLNELRAVDASEPNDVWAVGRTSSGFGEQPLVLHYDGIA
                     WSDVELPSEIHGVLNGVAAISPSDVWAVGSVGDPAASLERALVLHWDGTAWADVELGR
                     AAGGGKSALVDVEGSSPTDLWAVGYHHFRPLILRFDGEAWSQSPTEIRGTLLAIESFA
                     PSEAWAVGTPIQRFDGEAWTETPLAHGDAELVDVAAIGGLDIWAVGTRPSKEPGTTRA
                     AVYLYDGRRWVPVDGPSVAGSDALSAVDALPDGTLLAVGWKDVGLERETLAIRGTTCP
                     TAS"
     gene            1755..2930
                     /locus_tag="VIH70_02810"
     CDS             1755..2930
                     /locus_tag="VIH70_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8115324.1"
                     /translation="MDFRLTTEDLELRDSARSFTERVLIPLELECEEHHGLSSESSAN
                     AKQAVLDAGFNAINHARDDGGRGLDLLQQILIEEQWGRATGGLWDIPWRPSIPLANAT
                     PDQRERYLIPACRGERRDAYAITEEHAGSDPSMVRTSARRDGDAWVIDGTKWHVTSGD
                     VADFLLVHAHVDGDPAKPTVFLVDKDLPGVRLVRTPKYSHTFVFQHPIFAFEGVRVGL
                     DAVLGEVGAGYELTKDWFVQERIMIGARTMGAATRALDLSLAFAKERVQFGEPIVGFQ
                     AVEFMLADMAAQIMSAKSTLYRVGWQAARGGADRKELHALASAVKLVCSETAGRVADA
                     ALQIHGGRGYMREHPVERLWRELRVDRIWEGTSEIQRIVIGNELRKRGHEAYTGWPS"
     gene            2980..3957
                     /locus_tag="VIH70_02815"
     CDS             2980..3957
                     /locus_tag="VIH70_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8115325.1"
                     /translation="MEGGALGTITVGVDVGGTKIQSAAMRAKQVVGVHRLPTPLTGAK
                     DVVAAIGEAVAKALADGGAQPTDLEAVGMGVPGAIDTEAGTVSSSPNLPGFQAAEPVP
                     LGAMVAEALGGVPVRLDNDVRVAILGEWRRGAGHGYRNLLGVWVGTGVGGGLVLDGKV
                     YQGQGAAGEIGHMIVKPGGRACSDGRRGHLEAYAGRGQMEVQARRLVKEGHKTDLFEI
                     MAKRGRTRLSSGVWAVALEKKDKMARELVDDAVEALGVGLASVQNLLSLEAIVIGGGL
                     ADRLGPTFVDRIREAMRPLLFVPEQPPALLSSEFRDLSGAVGAGVLAGG"
     gene            complement(3997..4725)
                     /locus_tag="VIH70_02820"
     CDS             complement(3997..4725)
                     /locus_tag="VIH70_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115326.1"
                     /translation="MRQHRFATAFATAFSGALSGAFSGAFSGAIASVCLAFALAACTG
                     DAEPGAPSTTPPVTHISGTGPTASPTAPALPDEPPATRGPVAADCVRGWMTPPRDAQR
                     FAEPLRVIRRATGVEGPLEVVDLRFFAGPESPPSDKGYLLDVRRWYVKAFAKHDPSFQ
                     GRFLVESREFGTGLAAVAPYDTSGFRSPDWVGFQYDSGDPEARVYPGLPGTWDGVPYD
                     FVSGGAGLQIPGLPDEVVGCLSGI"
     gene            4783..>6035
                     /locus_tag="VIH70_02825"
     CDS             4783..>6035
                     /locus_tag="VIH70_02825"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8115327.1"
                     /translation="MQGASPQPPERSSYERLGHDESPLFSRRSFLRTTGFALAGATMY
                     ACTGGGRKQPVVAPGTTASVVATDTRWPIKRAIYVMMENRSFDNLYGRFPGANGATVG
                     VKFGQEVPLTASPDWLPGDIPHDRAAHLNDVNGGAYDGFGNGQFGDPWAYTQFEGASI
                     PNYWSWAKDYVLCDNFFASVGGPSYPNHFFFVAGQSGGALDNPENIETRQLSDGRTFK
                     SWGCDAVGKNVFVLVKDERGNLTKHDSCFTIRTVPEQLEDAGVSWAFYAAQPGQSGYF
                     WNALNGIDGVFHTDLWRPERIRSVDRLPQDIRAGRLPAVTWVTPRFELSDHPPESTCF
                     AHNWLTDIVNAVMETEDWEHTALFLTWDEWGGFYDHVPPPEVDDLGFGFRVPMLVMSP
                     YAKKGYVDDAEGEFSSPLRFVADNWG"
BASE COUNT          905 a         2080 c         2224 g          826 t
ORIGIN      
        1 cacgtacgcg gccgcgtctc gctcgctgaa ccgaccgatc gcggcggcgt cgcgctcgac
       61 gtcgccccac agcgtgagcg aggtcccgtc cgggaacggc acgaagtagt cgggcgtgat
      121 gatcgagacc tcgtacccga accgcttcag atcgagctcg cgcacgacct gcgggggcat
      181 cagggacacc acgtaggacg cgctcgagac ccggtagccc ggccacggct cctcggtcgc
      241 cgcggcgccc ccgacgacgc cccgtttctc caggacgagc acatccaggc cggccttggc
      301 aaggtacgct gcgcacgcga ggccgttgtg gcctcccccg atgatgacga cgtcgcgttc
      361 gaacacgccc acggaagccg gagggtaccg gccgcgcgat gtgccgcgca atggcggaca
      421 tcgtggcccg gcgctcggga accgcggcgc gcccgcgagt ggcccggcgc tcgggaaccg
      481 cggtgcgccc gcgagggtcc tgacggcgtg agcagcgtgc ggtggccgtg aacagcctcc
      541 agcggcgggg cctggtgaac gccgcggtcg cggccgtggc caccttcgcg atcggctcgg
      601 cggtggtcta cgcggccggt ggccccgacg acgtcgccgc gccgccccgc tccagccgcc
      661 cgtcggcctc cccgaagacc ccgtcctgcg cccccacctg ggccgtcgtt cggagcgcga
      721 acccggggga ggcggcgacc accctgcagg gggtcacggc cgtcacgtcg gcggaggcgt
      781 gggccgtcgg cgggctcggc gagcccgaga tcccgacggc ggtggcgatc cagcggtggg
      841 acggcgaccg gtggctggcc atcgccgggc cgagcccggg gtccaccctg aacgagctcc
      901 gtgccgtcga cgcctccgag ccgaacgacg tctgggccgt cggacggacc tcgagcgggt
      961 tcggcgagca gccgctggtc ctgcactacg acgggatcgc ctggtccgac gtcgagctcc
     1021 cgtcggagat ccacggcgtg ctgaacggcg tcgccgcgat ctcaccctcg gacgtgtggg
     1081 cggtcggatc cgtcggtgac ccggccgctt cgttggagcg ggcactggtc ctgcactggg
     1141 acgggacggc gtgggccgac gtggagctgg ggcgagcggc cggcggcggc aagtccgccc
     1201 tcgtcgacgt cgaagggagc tcacccaccg acctgtgggc ggtcggctac caccacttcc
     1261 ggccgttgat cctgcggttc gacggcgagg cgtggagcca gagcccgacc gagatccgcg
     1321 ggacgctgct cgcgatcgag tcgttcgccc cgagcgaggc atgggccgtc ggcacgccga
     1381 tccaacgctt cgacggcgag gcgtggacgg agacgccgct cgcccacgga gatgccgagc
     1441 tcgtcgacgt cgcggcgatc ggcggtctcg acatctgggc ggtgggcacg cgtccgtcga
     1501 aggagccggg gaccactcgc gcggccgtct acctgtacga cggtcgccgg tgggtgccgg
     1561 tcgacggacc atcggtcgcg gggtcggacg ccctctcggc ggtggacgcc cttcccgacg
     1621 ggaccctgct ggccgtggga tggaaggacg tcggcctcga acgcgagacc ctcgcgatcc
     1681 gcggcacgac ctgccccacc gcgtcgtgag cggaccggcg cggcgcatgc gcgttcgcta
     1741 ggctcgcccg gccgatggac ttccgcctca caaccgagga tctcgagctg agggatagcg
     1801 cccggagctt caccgaacgg gtgctgatcc ccctcgagct cgagtgcgag gagcaccatg
     1861 gactgagctc ggagagctcc gcgaacgcga agcaggcggt gctcgacgcg ggcttcaacg
     1921 cgatcaacca cgcgagggac gatggcggtc ggggcctcga cctcctccaa cagatcctga
     1981 tcgaggagca atggggccgg gccacgggcg ggctctggga catcccgtgg cggccgtcga
     2041 tccccctggc gaacgccacc cccgaccaga gggagcggta cctcatcccg gcgtgccggg
     2101 gcgagcggcg tgacgcgtac gcgatcaccg aggagcacgc cggttcggat ccgtcgatgg
     2161 tccgcacctc ggctcggcgc gacggcgacg cctgggtgat cgacggcacg aagtggcacg
     2221 tcacgtcggg cgacgtcgcc gacttcttgc tcgtgcacgc ccacgtggac ggcgaccccg
     2281 ccaagccgac cgtgttcctg gtcgacaagg acctgcccgg cgtccggtta gtccggacgc
     2341 cgaagtactc gcacacgttc gtgttccagc atccgatctt cgcgttcgag ggtgtccgcg
     2401 tcgggctcga cgcggtgctg ggcgaggtag gcgccggcta cgagctcacg aaggactggt
     2461 tcgtccagga gcggatcatg atcggcgcgc gcaccatggg cgcggccacc cgagccctcg
     2521 acctctcgct tgccttcgcg aaggagcgtg tccagttcgg cgagccgatc gtgggattcc
     2581 aggccgtcga attcatgctt gcggacatgg ccgcccagat catgtcggcg aaatcgacgc
     2641 tgtatcgcgt cggctggcag gcggcccgcg gtggcgccga tcgcaaggag ctgcacgcgc
     2701 tcgcgtcggc cgtcaagctc gtgtgctcgg agacggcggg tcgcgtggcc gacgcggccc
     2761 tccagatcca cgggggccgc ggctacatgc gcgagcaccc cgtcgaacgc ctgtggcgcg
     2821 agctcagggt cgaccgcatc tgggagggca cctccgagat ccagcggata gtcataggca
     2881 acgagctccg caagcgcggc cacgaggcct atacgggatg gccgtcctga ccgatactgg
     2941 cccgtagcgg ccgggtccgg ccagccacgc ttccccatcg tggaaggagg agcgctgggc
     3001 accatcacgg tgggcgtcga cgtcggcggg accaagatcc agtccgcggc gatgcgcgcg
     3061 aagcaggtgg tgggcgtgca tcgcctgccc acgccgctca cgggcgcgaa ggacgtggtg
     3121 gcggcgatcg gcgaggcggt cgcgaaggcg ctcgcggacg gcggggcgca gccgaccgac
     3181 ctcgaggcgg ttgggatggg ggtcccggga gcgatcgaca ccgaggccgg gaccgtctcg
     3241 agctcgccga acctcccggg cttccaggcg gccgaacccg tccccctggg ggcgatggtc
     3301 gccgaggcgc tcggcggcgt gccggtaagg ctcgacaacg acgtccgcgt cgcgatcctg
     3361 ggcgaatgga ggcgtggggc gggtcacggg taccgaaacc tgctcggcgt gtgggtcggc
     3421 accggtgttg gcggaggcct ggtgctcgac gggaaggtct accaggggca gggcgcggcc
     3481 ggggagatcg gccacatgat cgtgaagccg ggcggccgag cctgctccga cggccgccgc
     3541 ggacacctcg aggcctacgc cggccgaggc cagatggagg tgcaggcccg ccgcctggtg
     3601 aaggagggcc acaagaccga cctgttcgag atcatggcga agcggggacg gacacgtctg
     3661 tcgagcggcg tctgggccgt ggcgctcgag aagaaggaca agatggcacg cgaattggtc
     3721 gacgacgcgg tggaggctct cggcgtcggg ctcgcatcgg tccagaacct cctctcactc
     3781 gaggcgatcg tgatcggcgg cggcctcgcg gatcggctcg gccccacctt cgtggatcgg
     3841 atccgcgagg cgatgcggcc gctgctgttc gtccctgaac aacctcccgc gttgctctcg
     3901 agcgagttcc gcgacctgtc gggcgccgtg ggtgcggggg tgctcgccgg cggttagcgc
     3961 ggcgagggag ggatcgacct ggccaccgga ccgaggtcag atccccgaca ggcagccgac
     4021 cacctcgtcg ggcaaccccg ggatctgcag ccccgcacct ccgctcacga agtcgtaggg
     4081 caccccgtcc caggtccccg gcagccccgg atagaccctc gcctcggggt cgccgctgtc
     4141 gtactggaac ccgacccagt ccggcgaccg gaagccgctc gtgtcgtacg gggcgaccgc
     4201 cgccagaccg gtcccgaact cgcgggattc gaccaggaac cgtccctgga acgaggggtc
     4261 gtgcttcgcg aacgccttga cgtaccagcg ccggacgtcc aggaggtagc ccttgtccga
     4321 gggcggcgac tcgggccccg cgaagaagcg caggtccacc acctcgagtg gaccctcgac
     4381 gcccgttgcc cggcggatca cgcgcagcgg ttccgcgaag cgctgggcgt cgcggggcgg
     4441 cgtcatccac ccgcggacgc aatccgcggc gaccggcccc cgcgtggcgg gtggctcgtc
     4501 cgggagcgcg ggcgccgtgg gcgaggcggt cggacccgtc cccgagatgt gggtgaccgg
     4561 aggcgtcgtg gacggcgcgc cgggctcggc gtcgccggtg cacgcggcga gcgcgaacgc
     4621 gaggcacacg gatgcgatcg cgccagagaa cgcgccagag aacgcgccgg aaagcgcgcc
     4681 ggagaacgcg gtggcgaacg cggtggcgaa cctgtgctga cgcatgggcc gagggtcgga
     4741 gaaggatatc gttcgcgggg ttgcttgata gcttgggcaa ccgtgcaggg agcctccccc
     4801 caaccccccg agcgttcgag ctacgagcgt ctcggacacg acgaatcgcc gctgttctcc
     4861 cggcgatcgt tcctgcgcac gaccgggttc gccctggccg gggcgacgat gtacgcgtgc
     4921 accggcggcg gccgcaagca gccggtggtg gcgccgggca cgaccgcgtc ggtcgtggcg
     4981 accgacacgc ggtggccgat caagcgcgcg atctacgtga tgatggagaa ccggagcttc
     5041 gacaacctgt acggcaggtt ccccggcgcc aacggcgcga ccgtcggcgt caagttcggg
     5101 caggaggtgc cgctcacggc gagccccgac tggctcccgg gcgacatccc ccacgaccgg
     5161 gcggcgcacc tgaacgacgt gaacggcggt gcgtacgacg ggttcggcaa cgggcagttc
     5221 ggtgacccgt gggcctacac gcagttcgag ggggcatcga tcccgaacta ctggtcgtgg
     5281 gcgaaggact acgtgctgtg cgacaacttc ttcgcgtcgg tcggtggccc gtcctatccg
     5341 aaccacttct tcttcgtggc gggccagtcg gggggcgcgc tcgacaaccc ggagaacatc
     5401 gagacccgcc agctctcgga cggccgcacg ttcaagagct gggggtgcga cgcggtcggc
     5461 aagaacgtgt tcgtcctggt gaaggacgag cgcggaaacc tcaccaagca cgactcctgc
     5521 ttcacgatcc ggaccgttcc cgaacagctc gaggacgccg gcgtgtcctg ggcgttctac
     5581 gccgcgcagc caggccagtc cggctacttc tggaacgcgc tcaatggcat cgacggcgtc
     5641 ttccacaccg acctgtggcg acccgaacgg atccgctccg tagaccgact cccgcaggac
     5701 atccgcgccg gccggctccc cgcggtcacc tgggtcacgc cgcggttcga gctctcggat
     5761 cacccgcctg agagcacgtg tttcgcccac aactggctca ccgacatcgt caacgcggtg
     5821 atggagaccg aggactggga gcacacggcg ctgttcctga cgtgggacga gtggggaggg
     5881 ttctacgacc acgtgccgcc gcccgaggtc gacgacctcg ggttcgggtt ccgcgtcccg
     5941 atgctcgtga tgagcccgta cgcgaagaag ggctacgtcg acgacgccga gggcgagttc
     6001 tcgagcccgc tccggttcgt cgcggacaac tgggg
//
LOCUS       DATBRK010000096         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1888292, whole genome shotgun sequence.
ACCESSION   DATBRK010000096 DATBRK010000000
VERSION     DATBRK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888292"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1959)
                     /locus_tag="VIH70_02830"
     CDS             complement(<1..>1959)
                     /locus_tag="VIH70_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY8115328.1"
                     /translation="IKEHPVLLNRAPTLHRLGIQAFEPVLVEGKAIQIHPLVCTAFNA
                     DFDGDQMAVHVPLSAEAQAEARILMLSTNNILSPAHGRPIAVPTQDMVLGIYYLTLEP
                     DRPENDEDIPRFGSVNEALMALDHRLVRLHTVVKVRLPGKALPPEMRPDDVDRETSNG
                     DRVPVVETTVGRLIFNQSFPDSEEFVDRPLLKSDVSRLVDACVHRYDRAQVEQILDNL
                     KNVGFHYATRAGVTLGVEDVTTPSDKAVILDRHEKEASKIEAQYRKGIITDDERRQEL
                     ITIWTQATDEVKDAMEAQFGKTNPIYMMANSGARGNIMQIRQIAGMRGLVANPKGEII
                     PRPIKSNFREGLSVLEYFISTHGARKGLADTALRTADSGYLTRRLVDVSQELIVREED
                     CGTDRSVPVPVTYETPTGARVENQHLDTSLYGRVLAEDVKVERKKIASRGDFLDDAGA
                     QRLVEAGVDAVRVRSATTCEAEYGVCRLCYGWSLATGRLVDIGESVGIVAAQSIGEPG
                     TQLTMRTFHTGGVAGEDITHGLPRVVELFEARRPKGEAWITEIPGTVQIEDDEEKKER
                     RITVTSEDGSDSVQYKVGFRARLLVGDGDAVEVGQQLTEGSVNPHEKLRIEGVQALQN
                     HLVEEVQQVYRSQGVTIHDKHIELIVRQM"
BASE COUNT          278 a          684 c          648 g          349 t
ORIGIN      
        1 catctgacgc acgatcagct cgatgtgctt gtcgtggatc gtgacgccct gtgaacggta
       61 gacctgctgg acctcctcga cgaggtggtt ctgcagggcc tgcacgccct cgatccgcag
      121 cttctcgtgc gggttcaccg acccctcggt gagctgctgc ccgacctcga cggcgtcgcc
      181 gtcgccgacc agcagccggg cccggaaccc gaccttgtac tgcacggagt cgcttccgtc
      241 ctccgacgtg acggtgatcc gccgctcctt cttctcctcg tcgtcctcga tctggacggt
      301 gcccgggatc tcggtgatcc acgcctcgcc cttgggacgg cgggcctcga acagctccac
      361 gacacggggc agaccgtgcg tgatgtcctc acccgcgacg ccgcccgtgt ggaaggttcg
      421 catcgtgagc tgggtccccg gctcgccgat cgactgcgcg gccacgatcc ccaccgactc
      481 gccgatgtcc acgagccgcc ccgtcgcgag cgaccatccg tagcagagcc ggcagacgcc
      541 gtactccgcc tcgcaggtcg tcgccgaccg gacgcggacc gcgtcgacgc cggcctcgac
      601 caggcgctgc gcccccgcgt cgtcgaggaa gtcgccccgc gaggcgatct tcttgcgctc
      661 caccttgacg tcctcggcga gcacccggcc gtacagcgac gtgtcgaggt gctggttctc
      721 gactcgcgcg ccggtgggcg tctcgtacgt gacggggacc ggcaccgaac ggtcggtgcc
      781 gcaatcctcc tcccgcacga tcagttcctg cgagacgtcg acgagacgac gggtcaggta
      841 gcccgagtcg gccgttcgga gcgcggtgtc ggccaggccc ttgcgggcgc cgtgcgtcga
      901 gatgaagtac tcgagcaccg acaggccctc gcggaagttc gacttgatcg ggcgcgggat
      961 gatctcgccc ttcgggttgg ccaccaggcc ccgcatgccc gcgatctgac ggatctgcat
     1021 gatgttcccc cgggcacccg agttcgccat catgtagatc gggttggtct tgccgaactg
     1081 ggcctccatc gcgtccttga cctcgtcggt cgcctgggtc cagatcgtga tcagctcctg
     1141 gcggcgctcg tcgtcggtga tgatgccctt gcggtactgg gcctcgatct tcgacgcctc
     1201 cttctcatgg cggtccagga tcacggcctt gtccgagggc gtggtcacgt cctcgacccc
     1261 gagcgtcacg ccggcgcggg tcgcgtagtg gaacccaacg ttcttcaggt tgtcgaggat
     1321 ctgctcgacc tgcgcgcggt cgtagcggtg cacgcaggcg tcgaccagac gactcacgtc
     1381 gctcttgagc agcggacggt cgacgaactc ctccgagtcg gggaagctct ggttgaagat
     1441 cagccgcccg accgtggtct ccacgaccgg gacgcggtcc ccgttcgagg tctcccgatc
     1501 gacgtcgtcc ggccgcatct ccggagggag agccttgccc ggcaggcgaa ccttgacgac
     1561 cgtatgcagc cggaccagcc ggtgatcgag ggccatcagg gcctcgttca ccgagccgaa
     1621 cctggggatg tcctcgtcgt tctcgggacg atcgggctcg agcgtcaggt agtagatccc
     1681 gagcaccatg tcctgggtcg gcacggcgat cggacgaccg tgggccggcg acaggatgtt
     1741 gttggtcgac agcatcagga tgcgggcctc ggcctgcgcc tccgcggaga gcgggacgtg
     1801 gaccgccatc tggtcgccgt cgaagtcggc gttgaacgcc gtgcacacga gcgggtggat
     1861 ctggatcgcc ttgccctcga ccaggaccgg ctcgaacgcc tggatcccca gacggtggag
     1921 cgtcggcgcc cggttcagca gcaccggatg ctccttgat
//
LOCUS       DATBRK010000097         9074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_446835, whole genome shotgun sequence.
ACCESSION   DATBRK010000097 DATBRK010000000
VERSION     DATBRK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446835"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VIH70_02835"
     CDS             complement(<1..863)
                     /locus_tag="VIH70_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEY8115329.1"
                     /translation="MEWALLGSSLLVILGGAELFTNGVEWIGEGFGLSEGVVGSVLAA
                     VGTALPETILPLVAIVSGHASGEEIGIGAILGAPFMLTTLAMFVVGTAVLVFARGGRR
                     STDLHADPGVLRQDLGYFLCMYALAAAAGLVHVRALHWALALVLVIGYVFYVRRHLRA
                     PGERELEDEARSEIHPLYLWGWLRRARPSLDPWSGAPDWPMFVQTGLSLLLIVGGARM
                     FVSGVDTIAENFGVSHLVFALLVAPVATELPEKFNSVLWVRRRKDTLALGNMTGAMVF
                     QSSFPVTVGLLL"
     gene            1048..2394
                     /locus_tag="VIH70_02840"
     CDS             1048..2394
                     /locus_tag="VIH70_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HEY8115330.1"
                     /translation="MGRERGTTRITVAPDGEPPVGRGLTSVLLAIGVTIPAIALRIGG
                     ASLGHGFEALLFGLAIVGAAFILSWAAEVAQLDISAGLAIAVLALIAVLPEYAVDMVF
                     AWKGGNAFEQFGAACHPPGAPGEDACSLALANMTGANRLLIGIGWSMVVFIAAYRSRT
                     RPPVLREVRLDRQHSVEVAFLGLATVYSLTLPLKSQITLVDSLVLVSIFAAYTWRISQ
                     GPADEPHLVGPARWMGTFRPARRRVTVIAMFAFAATVILLSAEHFAEALVATGQEFGI
                     SEFLLVQWLAPLASEAPELLIAGLYAWRLNTNAGLGTLVSSKVNQWTLLVGTLPIVFA
                     IAAGGLHGLPVDAHQRQELFLTAAQSFFALAVLVTLSISVREAWLLFGLFWAQFLIGA
                     VVPQSFMGTERIIVATAYMILGVGIFARQRRLIRPLIRDGFATSYRDLGGRQVAGE"
     gene            2433..4682
                     /locus_tag="VIH70_02845"
     CDS             2433..4682
                     /locus_tag="VIH70_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8115331.1"
                     /translation="MDPRAFLERLSAEGEVVHVRDLPARTTEPGPFPVEVPELIRQRL
                     ALVGVTGLYAHQRAAFDLARSGGNVIVATGTASGKTLVYNLAFATAAVPDPKRTALYL
                     FPTKALARDQLRQVRELRLPQLRAGVYDGDTPRADRPLIRKNANLVMTNPDMLHAGIL
                     PDHPRWADFFLRLSHVVVDETHVFRGVFGSHVAQILRRLRRVVAHYGGDPAFVLASAT
                     VGNPVELAERLVGEPFEAFVDDRSPSGGKTFALWNPPIVDEESGARRSALTDTSFLMA
                     RLAEEGVKSIGFARSRRAAELLAEFTRRELDAELRPRVKAYRAGYLAEDRRELERRLA
                     NDELIAVASTNALELGIDIGSLDASLLVGYPGTRASMWQQAGRAGRRSDGSLAVLVAQ
                     DDPLDQYLVTHPADLFDKPSEAAVIDPTNPYVLIPHLLCAAREQPLSDEEVATYWPEA
                     SDTVLGLEATGELRRRGSLLHHAGHEAPHWKVDIRSSGGRRFQIVVEETGELLGTVDG
                     SRAFWQVHPGAVYLHQGEQFEVRVLDLEASVAIVTRSDPDYYTQSRDTTDIEVVIEQE
                     RERLSEDVQTTYGTVRVKNQVVAFARKHVATGEVLEIVPLDLPPVPLETRAVWWGIPQ
                     TVLDRAELRPSAIPGAAHAAEHAAIGLLPLVATCDRWDVGGVSTAYHPDTASCAIFVY
                     DGYQGGAGIAERGFSSARRWLQATLETVSRCPCAHGCPSCVQSPKCGNGNEPLDKAGA
                     VSLLEAMLG"
     gene            4699..5181
                     /locus_tag="VIH70_02850"
     CDS             4699..5181
                     /locus_tag="VIH70_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HEY8115332.1"
                     /translation="MTREVYIAVVGPSAGTPAELAIGEAVGRGVAEAGGVLVCGGMGG
                     VMEAAAGGCADAGGRSVGILPSDSRLDANPYLTIAVATGMGEARNAIVVRTADVVIAV
                     HGEFGTLSEIALALKMGKPVVGVGTWELGKEGEPVDAIVRAADARDAVSQALRLAGSL
                     "
     gene            5266..7011
                     /locus_tag="VIH70_02855"
     CDS             5266..7011
                     /locus_tag="VIH70_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEY8115333.1"
                     /translation="MTDHEEHPEIAPAPATDPHSDAELDAIAEAEVEGLPVSTDAALR
                     TTHGAGGRFRGAIDAVLPRGRFGDQRDILGGAGQNVIGLAVGVLTTFATQVIMTRGLG
                     PALFGVVTLATQFAFIASTATRFGMDVANVRLVAILVGRGESGRTRPLVRTSAIIAGV
                     ISVLVGVAVIVLADRLSTAFVSQWFHDQGVVAFDAAGVAIPFAALTWVYLGATRGLKI
                     MRHTLYIFWIGQPLGWIAIAVVSWFFAKTAGVTSFAYAASWAIACAFAWWAWERETRG
                     FDARTEGSGIPEERTGALMKFGMLRAPATLFAQLLFWIDTFVLAAYRSSAEVGVYGAT
                     VRTGQSLLLFLTSLSLVFSPFVADLHYRGEKAKLDALYKNVTRWGLATTLPILLALAI
                     LPGTIMRVYGPKFVAGANALEILIIGLIVPVIVGTVGFILIMVGRTGWDLLVYVASFV
                     VAVGIAVWLAPTYGMKGAAIAEAVTLTLSAFARLLLVKRFVDIWPFDRWFLRLIPPAL
                     AGAVVMWAVHAVLAGPKWLIDLTLSISAGTLAYGVVLLWIGLKPGERAPALALIRRLL
                     GRTPNPPDPNEGGEP"
     gene            7008..7373
                     /locus_tag="VIH70_02860"
     CDS             7008..7373
                     /locus_tag="VIH70_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8115334.1"
                     /translation="MTDPGRLERGRDKLSEVHGERALATVESLGDLGRLIVEVAYGDV
                     YSRPGLSLRERQITSVAALVATGRSSQLPVHLRSSLAAGLSPEELQEVIIQTATIAGF
                     PPAMNAWSTLKTIVAETET"
     gene            7373..7768
                     /locus_tag="VIH70_02865"
     CDS             7373..7768
                     /locus_tag="VIH70_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8115335.1"
                     /translation="MDALYGARRWADAWGRAWRTHDAELVGSLYADGASFRSQRFREL
                     DDPAGYARWAFDEEAEAECRFAEPALSGHDGAAVEYWAVSTARDGTVETIAGVALLRF
                     GADGRVVAERDYWNLAPGRAEPYEGWDPV"
     gene            7877..8668
                     /locus_tag="VIH70_02870"
     CDS             7877..8668
                     /locus_tag="VIH70_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115336.1"
                     /translation="MRRAILVPLVAMLATGLAACGYQPEIEPPGAATGGSSTPAPVAA
                     TGPTSAVPTFTADGCPIGDPAFCKQAAFLANALVLSDADAVFDLSRRVSMECADLDRD
                     VFPQCGNRDTLEGYVVGDHHGHLFVDGPVGYRRTLRFFVEAVDEEYSDEFGDARMQIL
                     GVSTCGKGANTSYHLVYLVGLGDPTSTLPADRFLGTYELTKEDGSWAIGATYVGLYTD
                     WQLVLDDPLTQIACGDIQPWVGAAPNLNPPQSESQPTPIAGTGST"
     gene            8760..9074
                     /locus_tag="VIH70_02875"
     CDS             8760..9074
                     /locus_tag="VIH70_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115337.1"
                     /translation="MADYVFLYSGGTMPETKEEQDAVMAAWNAWFGELGTTLKDGGNP
                     FTPAAKTIGSDGSVSDGGGSASGYSILTAGSLDEAVDKAKGCPVLKGGASITVYETFA
                     VM"
BASE COUNT         1251 a         3198 c         3251 g         1374 t
ORIGIN      
        1 agcagcagcc cgaccgtgac cgggaaggac gactggaaga ccatcgcacc cgtcatgttg
       61 ccgagcgcca gggtgtcctt gcggcgccgg acccacagca cgctgttgaa cttctccggg
      121 agctcggtgg ctacgggcgc caccagcagt gcgaagacga gatgcgacac gccgaagttc
      181 tccgcgatcg tgtcgacgcc cgacacgaac atcctcgcgc cgcccacgat cagcaggagc
      241 gagaggcccg tctggacgaa catcggccag tccggcgcgc ccgaccacgg gtccagcgac
      301 ggccgcgccc gacgcaacca gccccagagg tagagcgggt ggatctcgct gcgcgcctcg
      361 tcctcgagct cgcgctcccc gggcgcccgt aggtgccgcc ggacgtagaa cacgtagccg
      421 atcaccagca cgagggcgag cgcccagtgc agcgctcgga cgtgcacgag gccggcggcg
      481 gcggccagcg cgtacatgca gaggaagtac ccgaggtcct gtcgcaggac tcccgggtcc
      541 gcgtgcaggt cggtcgatcg ccgcccgccg cgcgcgaaca cgagcaccgc cgtgccgacg
      601 acgaacatcg cgagcgtcgt cagcatgaac ggcgctccga ggatcgcgcc gatgccgatc
      661 tcctcgccgc tcgcgtgacc ggacacgatc gccacgagcg gcaggatcgt ctcggggagc
      721 gcggtgccga ccgccgccag cacgctgccg acgacgccct cggacaggcc gaagccctcc
      781 ccgatccact cgaccccgtt ggtgaacagc tcggcgccgc cgaggatcac gagcaacgac
      841 gagccgagca gcgcccactc catcgggcgt gcatcctgtc acaccgccgg ccgtcgcctg
      901 cgccgagggg gcgccgagga ggctgttacg cttcgtcgga gaaggggagt agtccacaag
      961 cggatccgcc gtcatgacgg tcgcgcgtcg cgacccggtg gaccctcgcc cccggcgagg
     1021 acgagacctt cggccgagag gaaggccgtg ggtcgcgagc gcgggaccac ccggatcacc
     1081 gttgcgccgg acggcgaacc gcctgtcggc cgcggcctca cctcggtgct cctcgcgatc
     1141 ggcgtcacga tcccggcgat cgccctgcgg atcggcggcg cctcgctcgg tcacgggttc
     1201 gaggcgttgc tgttcgggct cgcgatcgtc ggggccgcct tcatcctgtc gtgggccgcc
     1261 gaggtggcgc agctcgacat ctcggcgggg ctcgcgatcg cggtgctggc gctgatcgcc
     1321 gtgctacccg agtacgcggt cgacatggtg ttcgcgtgga agggcgggaa cgcattcgag
     1381 cagttcggcg ccgcctgcca tccgcccggc gcccccggcg aggacgcgtg ctcgctggcg
     1441 ctcgcgaaca tgaccggcgc caatcggctc ctgatcggca tcggctggtc gatggtcgtg
     1501 ttcatcgcgg cctaccgttc acggacgcgg ccgcccgtgc tgcgcgaggt gcggctggac
     1561 cggcagcatt cggtcgaggt cgcattcctc ggcctcgcga ccgtctactc gctcacgctg
     1621 ccgctgaagt cacagatcac gctggtcgac tcgctggtcc tggtctcgat cttcgccgcg
     1681 tacacctggc gcatctcgca gggtccggcg gacgaaccgc acctcgtcgg tcccgcccgc
     1741 tggatgggga cgttccggcc cgcgcgccgc cgagtcaccg tgatcgcgat gttcgcgttc
     1801 gccgccacgg tgatcctgtt gtccgccgag cacttcgccg aggccctcgt cgcgaccggg
     1861 caggagttcg gcatcagcga gttcctcctg gtccagtggt tggccccgct cgcgtccgag
     1921 gcgcccgagc tgctgatcgc cgggctctac gcctggcgtc tgaacaccaa cgccggtctg
     1981 ggcaccctcg tgtcctcgaa ggtgaaccag tggaccctgc tggtcggcac cctgccgatc
     2041 gtgttcgcga tcgcggccgg cgggctgcac ggcctcccgg tcgacgcgca ccagcgtcag
     2101 gagctgttcc tcaccgcggc gcagtcgttc ttcgcgctcg ccgtcctcgt cacgctctcg
     2161 atcagcgtgc gcgaggcctg gttgctgttc ggcctgttct gggcgcagtt cctgatcggc
     2221 gcggtcgtgc cgcagtcctt catgggcacc gagcgcatca tcgtggcgac cgcgtacatg
     2281 atcctcgggg tcgggatctt cgcccgtcag cggcgcctga tccggccgct catccgcgac
     2341 ggtttcgcga cctcgtaccg cgacctcggt gggcgccagg tcgccggcga gtgaccccgg
     2401 cggtcctcgc gggcgccgct accatcggtc gagtggatcc ccgtgcgttc ctcgaacggc
     2461 tgagcgccga gggcgaggtc gtccacgtcc gcgacctacc ggcccgcacc accgagcctg
     2521 gccccttccc cgtcgaggtc cccgagctga tccgccagcg gctcgccctg gtcggcgtga
     2581 ccggcctgta cgcgcatcag cgggcggcgt tcgacctcgc gcgctcgggc gggaacgtga
     2641 tcgtcgcgac cggcacggcc tcgggcaaga ccctcgtcta caacctcgcg ttcgcgacgg
     2701 cggcggtgcc ggaccccaag cgcaccgcgc tgtacctgtt ccccaccaag gcgctggcgc
     2761 gcgaccaact ccgtcaggtc cgcgagctca ggctgcccca gctccgtgcg ggcgtctacg
     2821 acggcgacac accgcgggcc gaccggcccc tgatccgaaa gaacgcgaac ctggtcatga
     2881 cgaacccgga catgctccac gcggggatcc tgcccgacca tccgcgctgg gcggacttct
     2941 tcctgcgcct gtcgcacgtc gtggtcgacg agacccacgt gttccggggc gtgttcgggt
     3001 cgcacgtggc acagatcctg cggcggctcc ggcgcgtggt ggcgcactac ggcggcgacc
     3061 ccgcgttcgt cctcgcgtcc gcgaccgtcg gcaatccggt cgagctcgcc gaacggctcg
     3121 tcggggaacc gttcgaggcc ttcgtcgacg atcggtcgcc gagcgggggc aagaccttcg
     3181 cgctgtggaa cccaccgatc gtggacgagg agtcgggcgc ccgcaggagc gcgctcaccg
     3241 acacctcgtt cctgatggcg cggctcgccg aggagggcgt gaagtcgatc gggttcgcgc
     3301 ggtcccggcg ggcggcggag ctgctggccg agttcacgcg gcgcgagctg gacgccgaac
     3361 tgcgcccacg ggtcaaggcg tatcgcgccg gctacctggc cgaggatcga cgcgagctcg
     3421 aacggcgact cgcgaacgac gagctgatcg ccgtcgcctc gacgaacgcg ctcgagctcg
     3481 ggatcgacat cgggtcgttg gacgcgtcgc tgctggtggg ctacccaggg acgcgggcgt
     3541 cgatgtggca gcaggccggg cgcgcgggcc ggcgttcgga cggatcgctc gcggtgctgg
     3601 tggcgcagga cgacccgctc gaccagtatc tggtcactca ccccgcggat ctgttcgaca
     3661 agccctcgga ggccgcggtg atcgatccga cgaacccgta cgtcctcatc ccccatctgc
     3721 tgtgcgcggc ccgcgagcag ccgttgtccg acgaggaggt cgcgacctac tggccggagg
     3781 cctcggatac cgtcctgggt ctcgaggcga ccggcgagct ccggcgccgc ggctccctcc
     3841 tgcaccacgc gggccacgag gcgccccact ggaaggtgga catccgcagc agcggcgggc
     3901 gccggttcca gatcgtggtc gaggagacgg gggagctgct cggcacggtc gacggctcgc
     3961 gcgccttctg gcaggtccat ccgggcgccg tgtacctgca tcagggggag cagttcgagg
     4021 tacgggtgct ggatctggag gcgagcgtcg cgatcgtgac ccggtccgac cccgactact
     4081 acacgcagtc gcgcgacacc accgacatcg aggtcgtgat cgagcaggaa agggaacggc
     4141 tctccgagga cgtccagacg acctacggga cggtgcgggt caagaaccag gtcgtggcgt
     4201 tcgctcgaaa acacgtcgcc accggcgagg tgctcgagat cgttccactc gacctcccgc
     4261 ccgtgccgct cgagacgcgc gccgtctggt ggggcatccc gcagacggtg ctcgatcggg
     4321 ccgagctccg accgtcggcg atcccggggg cggcccacgc ggccgaacac gcggcgatcg
     4381 ggctgctccc gctggtcgcc acgtgcgatc gatgggacgt cggcggcgtg tcgaccgcgt
     4441 accatcccga caccgcgtcc tgcgcgatct tcgtgtacga cgggtaccag ggcggtgccg
     4501 gcatcgccga acgggggttc tcctccgccc ggcgatggct gcaggcgacg ctcgagaccg
     4561 tgtcgaggtg tccgtgtgcg cacgggtgcc cctcgtgcgt gcagtcaccc aagtgcggga
     4621 acgggaacga gccgctggac aaggcgggcg ccgtgtcgct gctggaggcg atgctgggtt
     4681 gacggtgagg acgcggagat gactcgagag gtctacatcg cggtggtggg accgtcggcg
     4741 gggacgccgg ccgagctcgc gatcggcgag gccgtgggac gaggggtcgc cgaggccggc
     4801 ggtgtcctgg tctgcggcgg gatgggcggc gtcatggagg cggcggcggg cggctgcgcc
     4861 gacgcgggcg ggcgttcggt ggggatcctc ccgagcgact cgcgactgga tgccaacccg
     4921 tatctgacga tcgcggtcgc gacggggatg ggtgaggcga gaaacgcgat cgtcgtgcgg
     4981 acggccgacg tcgtgatcgc ggtgcacggc gagttcggga cgctctcgga gatcgccctc
     5041 gccctcaaga tgggcaagcc ggtcgtcggc gtggggacct gggagctcgg caaggagggc
     5101 gaaccggtcg acgcaatcgt ccgggcggcc gacgccaggg acgcggtgtc gcaggcgctc
     5161 cggttggccg ggtccctcta gactcctcgc gaccaccgcc gcccggcgcc ggaccacccc
     5221 cttcctccgc gtgcggtccg ggttcgccgt ggagacaggg gacgcatgac cgaccacgaa
     5281 gagcatcccg agatcgcgcc cgccccggcg acggacccac actccgacgc cgagctcgac
     5341 gcgatcgccg aggccgaggt cgagggtctg ccggtatcga cagacgcggc gctccgcacg
     5401 acccatggcg ccggcggcag gttccggggg gcgattgacg ccgtcctgcc gcgggggcgc
     5461 ttcggcgacc agcgggacat cctcggcggc gccggccaga acgtgatcgg cctggcggtc
     5521 ggcgtgctca cgacgttcgc cacccaggtg atcatgacgc gcggcctggg ccccgcgctc
     5581 ttcggcgtcg tgaccctcgc aacgcagttc gcgttcatcg cgagcaccgc cacgcggttc
     5641 ggcatggacg tggcgaacgt ccgtctcgtg gcgatcctcg tcggccgcgg cgagtccggg
     5701 cggacacgcc cgctcgtacg gaccagcgcg atcatcgccg gcgtcatctc ggtcttggtt
     5761 ggcgtggcgg tcatcgtgct cgcggatcgg ctctcgaccg cgttcgtctc gcagtggttc
     5821 cacgaccagg gcgtggtggc gttcgatgcg gccggcgtcg cgatcccgtt cgccgcgctc
     5881 acctgggtct acctgggcgc cacacgcggg ctcaagatca tgcggcacac cctgtacatc
     5941 ttctggatcg gccagccgct cgggtggatc gccatcgccg tcgtgagctg gttcttcgcc
     6001 aagaccgccg gcgtgacctc cttcgcgtac gcggcgtcct gggcgatcgc atgcgcgttc
     6061 gcgtggtggg catgggaacg cgagacccgg gggttcgatg ctcggaccga gggctccggc
     6121 atccccgagg agcgcacggg ggcgctgatg aagttcggga tgctccgcgc ccccgcgacg
     6181 ctgttcgccc agctgctgtt ctggatcgac acgttcgtgt tggccgcgta ccggagttcc
     6241 gcggaggtcg gggtctacgg cgccaccgtg cggaccgggc agtcgctgct gctgttcctc
     6301 acctcgctct cgttggtgtt cagcccgttc gtcgccgacc tgcactaccg cggagagaag
     6361 gccaagctcg acgcgctgta caagaacgtg acgcggtggg gtctggccac cacgctgccg
     6421 atcctgctcg cgctcgcgat cttgccgggg acgatcatgc gcgtgtacgg cccgaagttc
     6481 gtcgccggcg ccaacgcgct cgagatcctg atcatcggcc tgatcgtgcc ggtgatcgtc
     6541 ggcacggtcg ggttcatcct gatcatggtc ggtcgcaccg gctgggacct gctcgtctac
     6601 gtcgcgtcgt tcgtcgtcgc ggtcgggatc gccgtctggc tcgcgcctac gtacgggatg
     6661 aagggcgccg cgatcgccga ggcggtgacg ctgaccctgt ccgcgttcgc gcggctgctg
     6721 ctcgtcaagc ggttcgtcga catctggccg ttcgaccgct ggttcctgcg gctgatcccg
     6781 ccggcgttgg cgggcgcggt cgtcatgtgg gcggtgcacg cggtcctggc ggggccgaag
     6841 tggctgatcg acctgacgct gtcgatctcc gccggcacgc tcgcgtacgg cgtggtgctg
     6901 ctctggatcg gcctcaagcc cggcgagcgg gcgcccgcgc tcgccctgat ccgcaggctg
     6961 ctcggccgca cgccgaaccc acccgatccg aacgaaggag gcgagccatg accgacccgg
     7021 gccggttgga acgcggccga gacaagctca gcgaggtgca cggggaacga gccctggcca
     7081 ccgtggagtc actgggcgac ctcgggcggc tgatcgtcga ggtggcgtac ggcgacgtct
     7141 actcgcgacc ggggctctcg ctccgcgagc gtcagatcac ctcggtcgcg gcgctcgtcg
     7201 cgaccggccg gtcctcgcag ctgccggtgc acctgcgctc gtcgctggcg gccgggctct
     7261 cgcccgagga actgcaggag gtgatcatcc agacggccac gatcgcgggc ttcccacccg
     7321 cgatgaacgc ctggtcgacg ctgaagacga tcgtcgcgga gacggagacc tgatggacgc
     7381 cctctacggc gctcggcgct gggcggatgc atggggccgt gcctggcgaa cgcacgacgc
     7441 cgagctggtg gggtcgctgt acgccgacgg tgcctcgttc cgttcccagc ggttccgcga
     7501 gcttgacgac ccggccggct acgcgcggtg ggcgttcgac gaggaggccg aggccgagtg
     7561 caggttcgcc gaacccgccc tgtcgggcca cgacggcgcc gccgtcgagt actgggcggt
     7621 cagcaccgct cgcgacggca cggtcgagac gatcgcgggg gtcgcactgc tgcggttcgg
     7681 ggccgatggc cgtgtggtgg ccgagcgcga ctactggaac ctcgcccccg gccgggccga
     7741 gccctacgag ggctgggatc ccgtctagcc ctgaccttcc tgcgcccgca ttcgcctgcg
     7801 cccgcattcg agcgcgggct tcgtcgaacg ggatcgaccc gagcctcccg cacggccgaa
     7861 cccgtatctt ccagcggtgc gtcgagcgat cctcgtcccg ctcgtagcga tgctcgcgac
     7921 gggcctcgcg gcctgcgggt accagcccga gatcgagcct cccggcgccg ccacgggcgg
     7981 ttcgtcgacg ccggcgccgg tcgcggccac cggaccgacc tcggcggtgc ccaccttcac
     8041 cgcggacggg tgccccatcg gcgacccggc gttctgcaaa caggcggcgt tcctcgccaa
     8101 cgcgctggtg ctgtccgacg ccgacgccgt cttcgacctc agccgccggg tgtcgatgga
     8161 gtgcgcggat ctcgatcgcg acgtgttccc ccagtgcggg aaccgagaca cgctcgaggg
     8221 ctacgtggtg ggcgaccacc atggtcacct cttcgtggac gggcccgtgg gctaccggcg
     8281 gaccctccgc ttcttcgtcg aagccgtgga cgaggagtac tcggacgagt tcggcgacgc
     8341 gcggatgcag atcctcggcg tctccacgtg cggcaagggc gcgaacacgt cctatcacct
     8401 ggtctacctc gtcgggctcg gcgacccgac cagcacgttg cccgccgatc gcttcctggg
     8461 gacctacgag ctcacgaagg aggacggctc gtgggccatc ggggccacct acgtcgggct
     8521 ctacacggac tggcagctcg tgctggacga ccccttgacc cagatcgcct gcggcgacat
     8581 ccagccgtgg gtcggggcgg ccccgaacct caacccgccg cagtccgaga gccaacccac
     8641 gccgatcgcc ggcaccggct cgacgtagtc cggcggccgc gcgcacgagt cgcgggcgac
     8701 ctgtcatgct ccggcgagcg cctcatggag ggcgacctcg gacggaaggg cgaccggcga
     8761 tggccgacta cgtgttcctg tacagcggcg gcacgatgcc ggagaccaag gaagagcagg
     8821 acgcggtgat ggccgcgtgg aacgcgtggt tcggcgagct gggcacaacg ctgaaggacg
     8881 gcggcaaccc gttcaccccg gcggccaaga cgatcgggtc cgacggctcg gtgagcgacg
     8941 gcggcgggag cgcgagcggc tactcgatcc tgacggccgg atcgctggac gaggcggtcg
     9001 acaaggccaa gggctgcccg gtgctcaagg gaggcgccag catcacggtg tacgagacct
     9061 tcgcggtgat gtag
//
LOCUS       DATBRK010000098        10209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1021988, whole genome shotgun sequence.
ACCESSION   DATBRK010000098 DATBRK010000000
VERSION     DATBRK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021988"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /gene="dapB"
                     /locus_tag="VIH70_02880"
     CDS             <1..717
                     /gene="dapB"
                     /locus_tag="VIH70_02880"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY8115338.1"
                     /translation="AAIDRSKIGQKIGQMIGHPEIDAPVSDELDRLLEANAEVAIDFT
                     HPDVAMDDIRWCISHAVHAVVGTTGISLENLQEIDKLLSDEGHESNVIVASNFALGAV
                     LMQRFAAQAVRYLPAVEIIELHHDQKADAPSGTAVATVRRIQEERDGSWRGPLAESLE
                     GVRGGDVEGIRVHSVRLPGLVAHHEVIFGGQGQTLTIRHDSTDRSSFMPGVLMATRAV
                     MVRPGLTIGLEPLLDLPPLP"
     gene            722..1498
                     /locus_tag="VIH70_02885"
     CDS             722..1498
                     /locus_tag="VIH70_02885"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY8115339.1"
                     /translation="MRRVACVFAHPDDDTFGVGGSLALHARDELELTVVLATSGEAGR
                     IADASLATREELGKVREAEDLASWRALGLEPDVRFLRHPDGGLGRLPRRGLVEQLRSI
                     LEELAPEVVVTFGPDGITGHPDHVAIGAAATDAFDTATADVPGAFLRLLHVAIAALDL
                     ERLNRGLRARGLEPIDSTQPFMPRPVPDREIGVRVDCSGVLDRKLEALRAHKTQSELE
                     DVPFDLWPDLLRTEAFVVAVPKRSPGDPVATDLLALPPAS"
     gene            1520..2728
                     /locus_tag="VIH70_02890"
     CDS             1520..2728
                     /locus_tag="VIH70_02890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8115340.1"
                     /translation="MRRITVDELELFLLASELASSDVPDRDEIERERSIAEPDRNFAA
                     FDGPDIVGTAGAFTMPITVPGGELVVGYPTLVGVLPTHRRRGIAAELMRALLDDARER
                     GELLSVLYASEGGIYGRYGYGVGTIGLTLRIETARSAFVRGYASTGQIRLVERERATK
                     EILAVHEATRLELPGMVHLDERRLAYVVGQEHGAGKERPTFFALREGPSGVDGYVIYK
                     VAHDWPENTPNTALHVRDLVAANPGAYADLWRFVLDVDLVTYVEASNRPSDEPLLYLL
                     REPRRLRARMSDNLWVRLVDVPGALAARRYAAAGRVVLEVADRFCAWNGGRYVLEAAE
                     DGRAFVEPTSEAAEIACTASDLGATYLGGTRFRQLRRAGRVDERAEGALARADAMFGW
                     DPAPWSPYDF"
     gene            complement(2751..3365)
                     /locus_tag="VIH70_02895"
     CDS             complement(2751..3365)
                     /locus_tag="VIH70_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY8115341.1"
                     /translation="MDASIPLAAFGLGVALGASPGPVQLLLFSEASRGGAGRGLRAMA
                     GANATFGLMMLLLAAGLSSLEPGETFLRVVKVIGGAFLVFIAVDAIRESRRPRVLDES
                     RGGLHPSVRGIVAVLLNPGVYVFLATTGTAVVASAVDEGGRGLAVVTVVALLVGVSLM
                     DSAMVVLGAGAHRVSERFLRILSDVLAVAMGALGVWLVIRGIAG"
     gene            complement(3371..3826)
                     /locus_tag="VIH70_02900"
     CDS             complement(3371..3826)
                     /locus_tag="VIH70_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8115342.1"
                     /translation="MRTLELRRHAQRDPEADRLSQQGRAQAQSVGATLAGGYSVVFVS
                     PAERAAETVAWFLRGLGEQLPAHAVVPGLAAEDNDGSPLAMAGVVAALLDAVPEGERG
                     LAISHTPLIERAVLGLGAAEIEPLAECEGVLLTRTGDDDPVEVAELRLR"
     gene            3871..4335
                     /locus_tag="VIH70_02905"
     CDS             3871..4335
                     /locus_tag="VIH70_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115343.1"
                     /translation="MKPTGRLVALLCLAIALGACTGGDASGDGGSTGSAGPSTTTEVP
                     TPAQTFTGAPGTATYEYANEGLLVTVDLDGSDGTLEVENDSEHDLDPPGLYVEDAVDG
                     HEIDGEVVDSAPVAAGETATFDVRLDGITVDDIGLLVLLFGADNYGAFVRTA"
     gene            complement(4332..4895)
                     /locus_tag="VIH70_02910"
     CDS             complement(4332..4895)
                     /locus_tag="VIH70_02910"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY8115344.1"
                     /translation="MPRIYTKTGDDGTTGLLYGGRVSKAELVPEACGTLDEAVAVLGL
                     ARAHTSDPSLSNELLALQRELFVVGADLATNPDGRARLEPGISLVSPDMVERLESRID
                     QLVEDHPLPNAFIVPGANPVSAFLDLARSTIRRAERRVVELRESDAAVNDEASRYLNR
                     LSDALFVLARVAAGEAEPHSRTDESPG"
     gene            5135..6019
                     /gene="dapA"
                     /locus_tag="VIH70_02915"
     CDS             5135..6019
                     /gene="dapA"
                     /locus_tag="VIH70_02915"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY8115345.1"
                     /translation="MAGRFGAVVTAMVTPFRNDHGLDLDAARSLAAHLYERGSDSLVI
                     AGSTGESPTLTHREKLDLFQAVIDVAKGKGKVLCGTGTYNTAETLELSREAEELGADG
                     LLLVTPYYNKPPQRGLLEHFTQVANAVSIPVVVYNIPGRTGTRIEHDTLLRIAEVPNI
                     VGVKDSTGDFQSISRLISEVSPDFEVYSGDDWATFGYLCLGAVGVVSVAGHLVGGRIR
                     QMCDLIEAGDIPAARKIHEELTPLFNALFITSNPIPVKTALEMVGLPVGPPRLPLVPA
                     TSQERERIRKALVDAGLL"
     gene            6003..7685
                     /locus_tag="VIH70_02920"
     CDS             6003..7685
                     /locus_tag="VIH70_02920"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY8115346.1"
                     /translation="MPGSSEAARPARVVFLGGVGEVGRNMACVELDGRILIVDVGLSF
                     PHAEMPGIDLVLPDFEYIRERFDDVEAIVLTHGHQDHIGAMPYLLRELDGDPLPVYGT
                     AFTLALLEGQLEEHQVRDRAEFRQVMPGEAAVAGPFSMRFLRVTHSIPDGMAVVIDTG
                     YGSILHTGDFKIDQTPLDGRATDLHALAEEAGRGVHLLLSDSTNAEESGYTDSERSVG
                     PVLQDIIARAPQLVVVACFSSHIHRIQQVVNAARADERVVAFLGRSMLQSVEAARLLG
                     ILHVPEDDVIPIEEVEHRDPSRVVVVCTGSQGEPFSALSLMAAREHKWIKLKEGDSVV
                     LSSSLIPGNEPAIHRVIDGLYRTGADVFHLPAYPVHASGHAAAEELRLMLSLVRPRWF
                     IPVHGERRHLAHHAKIATEVGIAADHVLICEDGDVVEVGEKVHVIERAPAGMTLVDGL
                     GIGDVGEVVLRDRRKLSADGVVVVVVAVDGHHGQVLSGPDIVNRGFVFDETSGDILEE
                     ARERVMLSLKDSAEAEVVDRGVLEQNIRRVLGKYFYEVTQRKPVILPVIVEV"
     gene            7686..9893
                     /locus_tag="VIH70_02925"
     CDS             7686..9893
                     /locus_tag="VIH70_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY8115347.1"
                     /translation="MASTTKGRAKRSSKRPSVRSHLRPWARDALGIGLVVLALLAILG
                     LWFGSAGPVGAGLDWLLRATFGMAAVVFPILALFWGLILLRGTAEQDRVRMFIGFAIT
                     TVGLLALISLWHGNPRPFGGYERLSGAAGIAGASASWPLGKLVSGIGVAIVWGGAVLL
                     GLLIFTGTPLSAIWDRIVDAFSRDGDGELDGEEEEEEQEQEVAEPRIVHASDGFSLLE
                     LPEDEEPEEELEPIAIPALVRPTGGGHYELPPLELLRTSPPSASSERDEEHQKAALER
                     TFHTFGVPAHVVAAHRGPTVTLYEVEIEAGTKVNKVLSLADDIAYALATPDVRIIAPI
                     PGRSAIGVEVPNKVRDFVMLGDILRSKTALDDDHPLAVALGKDVHGRAQLVNLADMPH
                     VLIAGATGAGKSSLINSFITSVLMRADPDEVKLVLVDPKRVELVHFADLPHLLVPVIV
                     HPKRAAEALAWVVREMEQRYELLAMVGVRDIDGYRAGMEEETLRIPPGQEDRVVDFPY
                     LLVVIDELADLMMVAPRDVEDAICRIAQMARAVGIHLVVATQRPSVDVVTGLIKANIP
                     SRIALMTSSQADSRVILDMNGAEKLVGHGDLLFAPSSASKPTRLQAAWVTEKEIQAVG
                     DWVRDQRSVEYAMSVEGLGKPPVDGEERGGLGGDDDLLEQAAELVIRSQLGSTSMLQR
                     KLKVGFARAGRLMDLMEDRGIVGPSLGSKARDVLVTPEEWPEKWEESRAAPTG"
BASE COUNT         1513 a         3531 c         3704 g         1461 t
ORIGIN      
        1 gccgcgatcg atcgctcgaa gatcgggcag aagatcgggc agatgatcgg acaccccgag
       61 atcgacgcgc cggtctccga cgagctcgat cggctgctcg aggccaacgc cgaggtcgcg
      121 atcgacttca cgcacccgga cgtcgcgatg gacgacatcc gctggtgcat ctcgcacgcg
      181 gtgcacgccg tggtcggaac gacggggatc tcgctcgaga acctccagga gatcgacaag
      241 ctgctctcgg acgagggtca cgaatcgaac gtgatcgtcg cgtcgaactt cgcgctcggc
      301 gccgtgctga tgcagcgctt cgccgcgcag gcggtccgct acctgccggc cgtcgagatc
      361 atcgagctcc accacgacca gaaggcggac gctccctcgg ggaccgcggt cgccacggtc
      421 cggcggatcc aggaggagcg ggacgggtcg tggcgcggcc cgctcgccga gtcgctcgag
      481 ggggtgcgcg ggggcgacgt cgaggggatc cgcgtccatt cggtgcgtct gcccgggctc
      541 gtggcgcacc atgaggtcat cttcggcggg cagggacaga cgctcacgat ccggcacgac
      601 tcgaccgatc ggtcctcgtt catgccgggc gtgctgatgg ccacccgggc cgtgatggtc
      661 cgcccggggc tcacgattgg gctcgagccg ctgctcgacc tcccgcccct gccgtagccc
      721 ggtgcgacgg gtcgcgtgcg tcttcgcgca tcccgacgac gacacgttcg gcgtgggcgg
      781 cagcctcgcg ctccacgcgc gggacgagct cgagctcacc gtggtccttg cgacgagcgg
      841 ggaggccggc cgcatcgcag acgcctcgct cgcgacccgc gaggaactcg ggaaggtgcg
      901 cgaggcagag gatctcgcgt cctggcgggc gttggggctg gagcccgatg tccgtttcct
      961 gcgacacccc gatggcgggc tcgggcggtt gccgcgccgg gggctggtcg agcagcttcg
     1021 ctcgatcctc gaggagttgg cgccggaggt tgtggtcacg tttggacccg acgggatcac
     1081 gggccatccg gaccacgtgg cgatcggtgc ggcggccacc gacgcgttcg acacggcgac
     1141 ggcggacgtt cccggcgcgt tcctgcggct gctgcatgtc gcgatcgcgg ccctcgatct
     1201 ggagcggctg aatcggggcc tgcgtgcgcg cgggctcgaa ccgatcgact ccacccagcc
     1261 cttcatgcct cggccggtgc ccgaccggga gatcggcgtg cgcgtcgact gctccggcgt
     1321 gctcgatcgc aagctcgagg cgctgcgcgc gcacaagacc cagtcggagc tggaggatgt
     1381 ccctttcgac ctgtggcccg acctgctccg gaccgaggcg ttcgtggtcg ccgtgccgaa
     1441 gcggtctccg ggggacccgg tcgccaccga cctgctggcg ctcccgccgg cctcgtaagc
     1501 tccgcggatg ggccaacggg tgcgcaggat cacggtggac gagctcgagc tgttcctcct
     1561 ggcctccgag ctcgcgtcca gcgacgttcc cgatagggac gagatcgaac gcgagcgctc
     1621 gatcgccgaa cccgaccgga acttcgcggc cttcgacggg ccggacatcg tggggacggc
     1681 gggcgcattc acgatgccga tcacggtccc cggcggcgag ctcgtcgtcg ggtacccgac
     1741 cctggtcggc gtgctcccca cgcaccggcg gcgggggatc gcggcggagc tgatgcgagc
     1801 gttgctcgac gacgcgcggg agcgcggcga gctgctctcg gtgctgtacg cctcggaggg
     1861 cggcatctac ggccggtacg ggtacggggt cgggacgatc gggctgacgc ttcggatcga
     1921 gaccgcccgc tcggcgttcg tccgcgggta cgcgtcgacc ggccagatcc ggctggtcga
     1981 gcgcgaacgc gccaccaaag agatcctcgc cgtgcacgag gccacgcgcc tcgaactccc
     2041 ggggatggtc cacctggacg agcggcggct cgcgtacgtc gtcggccagg agcacggcgc
     2101 cggcaaggaa cggccgacgt tcttcgcgct gcgcgagggt ccgtccggcg tcgacggata
     2161 cgtgatctac aaggtcgcgc atgattggcc cgagaacacg ccgaacaccg cgctgcacgt
     2221 tcgcgacctc gtggccgcga acccgggagc gtacgcggac ctgtggcggt tcgtgctgga
     2281 cgtggacctc gtgacgtacg tcgaggcatc gaaccgaccg agcgacgaac cgctcctgta
     2341 cctgctccgc gagcctcgac gccttcgcgc caggatgtcc gacaacctgt gggtgcggct
     2401 ggtcgacgtc cccggtgccc tggccgcccg acggtacgcg gcggccggcc gcgtcgtcct
     2461 cgaggtcgcc gaccgattct gcgcgtggaa cgggggccgg tacgtgctcg aggccgcgga
     2521 ggatggccgc gccttcgtgg agccgacgag cgaggcggcc gagatcgcgt gcacggcgag
     2581 cgatctcggc gccacgtacc tgggcggaac gaggttccgc cagctccgcc gcgccggccg
     2641 ggtcgacgaa cgcgccgagg gcgcgctcgc gcgggccgac gccatgttcg gctgggaccc
     2701 ggcgccgtgg tccccgtacg acttctgacc ggcatacgat agcgcccgcg tcagccggcg
     2761 atcccgcgga tcacgagcca gacgccgagg gcgcccatcg cgaccgccag gacgtcgctc
     2821 aggatccgca ggaagcgctc gctcacgcgg tgcgcgcccg cgccgagcac caccatcgcc
     2881 gagtccatca gcgacacgcc caccaacagc gcgaccaccg tgaccaccgc gagcccgcga
     2941 ccgccctcgt cgacggcgct cgcgaccacc gcggtgcccg tggtcgcgag gaacacgtag
     3001 acgcccgggt tcagcaggac ggcgacgatc ccccgaacgc tggggtgcaa cccgcctcgg
     3061 gattcgtcga gcacccgtgg gcgacggctc tcgcggatcg cgtcgaccgc gatgaacacc
     3121 aggaacgcgc ccccgatcac cttgacgacg cgcaggaacg tctcgccggg ctcgagtgag
     3181 gacagccccg ccgcgagcag cagcatcatc aggccgaacg tcgcgttcgc gccggccatc
     3241 gcgcgcaggc cgcgccccgc gccaccgcgc gaggcctcgg agaacaggag gagctgtacc
     3301 ggtcccggcg acgctccgag ggcgacgccg aggccgaacg cggcgagcgg gatcgaggcg
     3361 tccaccgcgg ctagcggagg cgcagttccg cgacctcgac cgggtcatcg tcgccggtcc
     3421 tggtgagcag gacgccctcg cactcggcga gcggctcgat ctcggccgcg cccagtccca
     3481 ggaccgcgcg ctcgatcagc ggcgtgtgcg agatcgcgag cccgcgctcg ccctccggaa
     3541 ccgcgtcgag caacgccgcg acgacgccgg ccatcgcgag cggcgatccg tcgttgtcct
     3601 ccgcggccag acccgggacg acggcgtggg cggggagctg ctcgccgaga ccgcggagga
     3661 accacgcgac ggtctcggcc gcccgctcgg cgggggagac gaacacgacg gagtagccgc
     3721 ccgcgagcgt cgcccccacc gactgtgcct gcgcccggcc ctgctgcgat agccgatcgg
     3781 cctccgggtc acgctgcgcg tggcgtcgca gttcgagcgt cctcacgtgg gtcttcctcc
     3841 ttcggcgggt catgtagcat gccgcgaccc atgaagccta ccggtcgtct cgtcgcgctc
     3901 ctgtgcctgg cgatcgcgct cggggcctgc accggagggg acgccagcgg ggacggcgga
     3961 tcgacggggt cagcgggccc gagcacgacc acggaggtcc cgacccccgc ccagacgttc
     4021 acgggtgccc ccggcaccgc gacatacgag tacgccaacg agggtctgct cgtgacggtc
     4081 gacctcgacg ggagcgacgg gaccctcgag gtcgagaacg actccgagca cgacctcgac
     4141 cccccgggtc tctacgtcga ggacgcggtc gacggccacg agatcgacgg cgaggtcgtg
     4201 gacagcgcac ccgtggcggc gggagagacc gcgaccttcg acgtgaggct ggacggcatc
     4261 acggtcgacg acatcgggct gctggttctg ctgttcggcg ccgacaacta cggcgcgttc
     4321 gtccggaccg cctagccggg cgattcgtcg gtgcggctgt gcggctcggc ctcgcccgcc
     4381 gcgacgcgcg ccaggacgaa gagcgcatcc gacaggcggt tcaggtagcg ggacgcctcg
     4441 tcgttgaccg cggcatccga ctcccggagc tcgaccacgc gccgctcggc ccggcggatc
     4501 gtgctccgcg cgaggtcgag gaacgccgag acggggttcg cgccggggac gatgaaggcg
     4561 ttcgggaggg ggtggtcctc gacgagctgg tcgatccgcg actccagccg ctcgaccatg
     4621 tccggcgaga ccagggagat gccgggttcg agcctggcgc gcccgtcggg gttggtggcg
     4681 aggtcggccc cgaccacgaa gagctcccgc tgcagcgcca ggagctcgtt cgagagcgag
     4741 gggtcgctcg tgtgggccct cgcgagaccg agcacggcga cggcctcgtc gagcgtgccg
     4801 caggcctcgg ggacgagttc ggccttggac acccggcccc cgtagaggag acccgtggtg
     4861 ccgtcgtcac cggtcttggt gtagatgcgc ggcatggtgg ctaggatagc ggcgcctccg
     4921 cccgagccgg gcggtcgcac cgtccggccg agcacgagag cggagcccga acaggagtcg
     4981 caggagatgc cggaggcacg agcgacgctc cttccgcacc tgcacaacgt tgctcggtac
     5041 tctctcgaca cccctgaatg acatgtgcaa catgtggcag atgtggttat cgtgcggacc
     5101 gtggaacacg cccgcaccgg agtgaggagg catcatggcc ggtaggttcg gggcggtcgt
     5161 caccgccatg gtcacaccct tccgcaacga tcacgggctc gacctggacg cggcgcgctc
     5221 gctcgccgca cacctgtacg agcgcggctc ggattcgctc gtcatcgcgg gttcgaccgg
     5281 cgagtccccc acgctcaccc accgggagaa gctggacctg ttccaggcgg tgatcgacgt
     5341 cgccaagggc aaggggaagg tgctgtgcgg aacgggcacc tacaacaccg ccgagacgct
     5401 cgagctgtca cgcgaggccg aggagctcgg cgccgacggg ctcctgctcg tgacgcccta
     5461 ctacaacaag cccccgcagc gaggactgct cgagcacttc acgcaggtcg cgaacgccgt
     5521 ctcgatcccg gtggtcgtgt acaacatccc cgggcggacc ggaacccgca tcgaacacga
     5581 cacgctgctg cggatcgccg aggtgccgaa catcgtcggc gttaaggact cgacgggcga
     5641 cttccagtcg atctcgaggt tgatctcgga ggtctcgccc gacttcgagg tctacagcgg
     5701 cgacgactgg gcgacgttcg gctacctgtg cctgggcgcg gtcggggtcg tctcggtggc
     5761 cggtcacctc gtgggcggga ggatccgcca gatgtgcgac ctcatcgagg cgggcgacat
     5821 cccggcggcg cgcaagatcc acgaggagct gacgcccctg ttcaacgcgc tgttcatcac
     5881 ctcgaacccg atcccggtca agaccgcgct cgagatggtc gggctccccg tcggcccgcc
     5941 gcggctcccg ctcgtgccgg cgacctcgca ggagcgggag cggatccgca aggccctggt
     6001 ggatgccggg ctcctctgaa gccgcccggc ccgctcgcgt cgtcttcctc ggaggggtcg
     6061 gcgaggtggg tcggaacatg gcctgcgtcg agctcgacgg ccgcatcctg atcgtcgacg
     6121 tcggcctgtc gttcccgcac gccgagatgc ccggcatcga cctcgtcctg cccgacttcg
     6181 agtacatacg ggaacggttc gacgacgtcg aggccatcgt cctgacgcac gggcatcagg
     6241 accacatcgg cgccatgccg tacctgctgc gcgagctcga cggggacccg ctgccggtgt
     6301 acgggacggc gttcacgctc gcgctgctcg aggggcagct ggaggagcac caggtccgcg
     6361 accgcgccga gttccggcag gtgatgcccg gggaggccgc ggtggccgga ccgttctcga
     6421 tgcggttcct ccgcgtcacg cactcgatcc cggacggcat ggcggtggtg atcgacaccg
     6481 gctacggctc gatcctgcac accggcgact tcaagatcga ccagaccccg ctggacgggc
     6541 gcgccaccga cctgcacgcg ctcgccgagg aggcgggaag gggcgtgcac ctgctgctca
     6601 gcgactcgac gaacgccgag gagtccgggt acacggacag cgagcggagc gtgggcccgg
     6661 tcctgcagga catcatcgcc cgggccccgc agctcgtcgt ggtcgcgtgc ttctcgagcc
     6721 acatccaccg gatccagcag gtggtgaacg ccgcccgggc cgacgagcgc gtggtcgcgt
     6781 tcctcggccg ctcgatgctc caaagcgtcg aagcggctcg gctgctcggg atcctgcacg
     6841 tgcccgagga cgacgtgatc ccgatcgagg aggtcgagca ccgcgacccg tcccgcgtgg
     6901 tcgtcgtctg cacgggctcg cagggggagc cgttctcggc gctcagcctg atggccgcgc
     6961 gcgagcacaa gtggatcaag ctcaaggagg gcgacagcgt cgtgctgtcg tccagcctga
     7021 tcccgggcaa cgagcccgcg atccaccgcg tgatcgacgg gctctatcgg accggcgcgg
     7081 acgtgttcca ccttccggcg tacccggtac acgcgagcgg ccacgccgcc gccgaggagc
     7141 tccgcctgat gctctccctc gtgcgcccga gatggttcat cccggtccac ggcgagcgac
     7201 gtcacctcgc ccaccacgcg aagatcgcga ccgaggtcgg catcgccgcc gaccacgtcc
     7261 tgatctgcga ggacggagac gtggtcgagg tcggggagaa ggtccacgtg atcgagcggg
     7321 cgccggccgg gatgacgctc gtcgacgggc tcggcatcgg cgacgtcggc gaggtggtcc
     7381 tgcgggaccg ccgcaagctg tcggccgacg gggtggtggt ggtcgtggtg gcggtcgacg
     7441 ggcaccacgg gcaggtgctc tccggcccgg acatcgtgaa ccgcgggttc gtgttcgacg
     7501 agacctcggg cgacatcctt gaagaggcgc gcgaacgcgt catgctttcc ctgaaggaca
     7561 gcgccgaggc cgaggtggtc gaccgcgggg tgctcgagca gaacatccgg cgggtcctcg
     7621 gcaagtactt ctacgaggtc acgcagcgta agcccgtgat cctgcccgtg atcgtggagg
     7681 tgtagttggc gagcaccacg aagggccgcg cgaagaggag cagcaagcgg ccctcggtca
     7741 ggagccacct tcgtccctgg gcccgcgacg ccctcgggat cggtctggtc gtgctcgcgc
     7801 tcctcgcgat cctcggactg tggttcggtt cggccgggcc ggtgggggcg ggcctggact
     7861 ggctgctccg cgccaccttc ggcatggcgg cggtcgtctt cccgatcctc gccctgttct
     7921 gggggctgat cctgctccgg gggacggccg agcaggaccg tgtgcggatg ttcatcgggt
     7981 tcgcgatcac gaccgtcggc ctgctcgccc tgatctcgct ctggcacggg aacccgaggc
     8041 cgttcggcgg ctacgagcgc ctgagcggtg ccgccggcat cgcgggcgcg tcggcctcgt
     8101 ggccgctcgg aaagctggtc tccgggatcg gcgtcgcgat cgtgtggggc ggcgcggtcc
     8161 tcctcggcct gctgatcttc acggggaccc cgctctcggc gatctgggac cgcatcgtgg
     8221 acgccttctc gagggacggc gacggcgagc tcgacgggga agaggaagaa gaggaacagg
     8281 agcaggaggt cgccgaaccg cggatcgtgc acgcgagcga cggcttctcg ctgctcgagc
     8341 tccccgagga cgaggagccg gaggaggagc tcgagccgat cgcgattccc gcgctcgtcc
     8401 ggccgactgg aggcgggcac tacgaactcc cgccgctcga gctgctccga acatctccgc
     8461 cgtcggcgtc cagcgaacgg gacgaggagc accagaaggc cgcgctcgag cgcacgtttc
     8521 acacgttcgg cgtgcccgcc cacgtcgtgg cggcgcaccg ggggccgacc gtcaccctgt
     8581 acgaggtcga gatcgaggcc ggcacgaagg tgaacaaggt gctctcgctg gccgacgaca
     8641 tcgcgtacgc gctcgcgacc cccgacgtgc ggatcatcgc gccgatcccc ggtcgctccg
     8701 cgatcggggt cgaggtgccg aacaaggtcc gcgacttcgt gatgctcggc gacatccttc
     8761 gctcgaagac ggcgctcgac gacgaccacc cgctggcggt cgcgctcggc aaggatgtcc
     8821 acggacgggc gcagctcgtc aacctcgcgg acatgcccca cgtgctgatc gccggggcga
     8881 ccggcgccgg gaagtcgagc ctgatcaatt cgttcatcac ctcggtcctg atgcgcgccg
     8941 atcccgatga ggtcaagctc gtgctggtcg accccaagcg cgtcgagctc gtgcacttcg
     9001 ccgacctgcc tcacctgctc gtcccggtga tcgtgcaccc caagcgcgcc gccgaggcac
     9061 tcgcttgggt ggtacgcgag atggagcagc gatacgagct gctcgcgatg gtgggggtcc
     9121 gcgacatcga cggctaccgc gcggggatgg aggaggagac gctgcggatc ccgccggggc
     9181 aggaggaccg cgtcgtcgac ttcccctacc tgctggtcgt gatcgacgag ctcgccgacc
     9241 tgatgatggt cgcgccgcgc gacgtcgagg atgcgatctg ccggatcgcc cagatggcgc
     9301 gcgccgtggg gatccacctg gtcgtcgcca cgcagcgccc cagcgtcgac gtcgtgaccg
     9361 ggctgatcaa ggcgaacatc ccgagccgca tcgcgctcat gacctcgagc caggccgact
     9421 cgcgcgtgat cctggacatg aacggcgccg agaagctggt cgggcacggc gacctcctct
     9481 tcgcgccgtc gtcggcctcc aagccgacgc gcctgcaggc ggcgtgggtg acggagaagg
     9541 agatccaggc ggtgggggac tgggtccgcg accagcgctc ggtcgagtac gcgatgagcg
     9601 tcgagggcct ggggaagccg ccggtggacg gcgaggagcg cggcgggctc gggggtgacg
     9661 acgacctgct cgagcaggcg gccgagctcg tgatccgctc gcagctcgga tccaccagca
     9721 tgctgcagcg caagctgaag gtcgggttcg cgcgcgccgg gcggctgatg gacctgatgg
     9781 aggaccgcgg gatcgtcggt ccgtcgctcg ggtcgaaggc gcgcgacgtg ctcgtgacgc
     9841 cggaagagtg gccggaaaag tgggaggagt cccgcgccgc gccaactggg tagggcacgg
     9901 ttcgccgggt ggtttcgagg agttctcgtc tcgcgtgccc ggtcggcggt cgtccgatag
     9961 gatgcccgcg atccggccgg gaggtttctt gcagcgaacg accgtcagcg tgggttccgc
    10021 gctccgaaag gtgcgcgagg cgcgtggcgt gacactggag gaggcggcac gagacacgcg
    10081 cgtgcggctc gaattcctcg aggcgatcga ggccgaggac ttccagctgc tcctgggtga
    10141 ggtccacctg cgcgggtgcc tgcggaccta cgccacgtac ctcaggctgt ccccggacaa
    10201 ggtcgtcgc
//
LOCUS       DATBRK010000099         7098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2755048, whole genome shotgun sequence.
ACCESSION   DATBRK010000099 DATBRK010000000
VERSION     DATBRK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2755048"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VIH70_02930"
     CDS             complement(<1..268)
                     /locus_tag="VIH70_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY8115348.1"
                     /translation="MDPRIGTMFAGHRIEEVIGRGGASVVYLAEHLRLGRRVALKILA
                     PHLAEDEMFRERFIRESRIAAGLDHPNVVTIYDAGEVEDGLYISM"
     gene            401..3145
                     /locus_tag="VIH70_02935"
     CDS             401..3145
                     /locus_tag="VIH70_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8115349.1"
                     /translation="MIVGRTAEIASIASFLDDVPDGPTALVLDGEPGIGKTVLWARAV
                     EEARGRGYRVMSCRPAGPDAQLSFVALGDLFSADTEPLFETLPQPQREALEVALLRRH
                     RGGDAPDPRAAAVATLGVVRAATAQAPSIIAIDDVQWLDPATARVLAFALRRLTAERI
                     GVVVSRPPTGEATPLGLADTMHGEGFRTLRVGPLGAHEIGVIVRDRTGVTLSKPDVER
                     LAELCGGNPFYALEIARARERGDEGVTGQRLPIPKSVHEDLVRARVDSVSATTREVLL
                     FAASISRPTVELLRRAIPATTLNPGLQGAIDAGLIEVLGADVRFIHPLYRSSIYADTS
                     RRRRHEMHARLAEITPDPEERARHLALAADGSDGKIAAEVEAAAASARGRGAPEVAAD
                     LVEHAIRLTPSDDTASLRRRLLVSAQDRMSAGDGDGARERAREALRLSEPGVAQAEPL
                     RRIGASEFARGALAEARATLAEALARAGEDDRASAEIHRELAMIGIRSGEVGAAERDA
                     AAALERAGRTGDAQLTEAIATAAAEVDLLLGRSSSDLSQVFNGYVAPDAPLSGSPELV
                     AALAEAFAGQLDRSERRLERLRRQAHDRGDEPGRLLVTTRLAELRIRAGSWDRAATLA
                     HEAYELALDLDTSHGLQLGLMASAAAGSGRIDDARRFAEQGLTSASGDRPAVIWNLAA
                     LGFLELSLARYADALRVLGRAGGLLAETGIADPSAFPFLADEAEALIGVGELATASRR
                     IAALEEASDRLDRDLIRGQAARCTGLASAAEGDIPASLRELERAVEIHERLGVAIELG
                     RSLLALGMSRRRDRQKRPAREVLERALEAFTTTETPLWANRVRDEIARIGGRRASVGE
                     LTESEAQVARLAAAGLTNREIARTLSIGIRTVEGHLSHVYTKLEIRSRTELAAFDLDV
                     "
     gene            3308..4729
                     /locus_tag="VIH70_02940"
     CDS             3308..4729
                     /locus_tag="VIH70_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY8115350.1"
                     /translation="MFSKFLSSRAWLVAFVMFATAFPVVPLAEGEVPIPQGSRTSPPN
                     IVLILTDDQRADTLRYMPIVQRLLVQTGVRFDNGYVANPLCCPSRASILTGTYSHTNG
                     VYSNHGPHGGFAAFHDATTIATVLQAAGYRTGQFGKYFNGYRDTTYVPPGWDRWFATY
                     DRDSGYYGYTAVSDGDEVQYGSDPADYGQTILRREAVSFIEDTDASKPLFMYWATHAP
                     HAPAIPERQDRKAFASIERWRSPSYDEADVSDKPSHVQARPPIDPATAREIDRFRLDQ
                     IRSLQSVDRSVGAIVKALRESGRLENTIIVFMSDNGMLWGEHRLHGKSEIYEEAIAVP
                     FVVRYDAMIGRARTDEHLVLNIDLAPTFADAAGVELPNADGRSLLPLLDSSDAGWRES
                     FLVEHLRAGDNTRYAPTFCAVHTDRYVLVRYATGEEEMYDLAHDPAQLENVAGSARYD
                     ELRGSLRGRLRVLCDPLPPGFSF"
     gene            4795..5466
                     /locus_tag="VIH70_02945"
     CDS             4795..5466
                     /locus_tag="VIH70_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8115351.1"
                     /translation="MARRGDLNGWWWFGIAVVGFSCRVLFRLRIEGADRVPSVGPAIV
                     AGNHVSALDGVVLALTTSSRSRRMTRFLVAAEFFRKLWFGWALRLYGQIPLRRGQRDQ
                     GALDVAIDTIRGGALAGIFPEGTVNPEPGAGLMRGRKGAARIALASVATVVPVGIWGT
                     QARWPRSGLHFRRPWRPVVAISYGEPIPPKGDPDSAPDVKAFTDVILDALAAQTERAK
                     ELAEA"
     gene            complement(5471..6088)
                     /locus_tag="VIH70_02950"
                     /pseudo
     CDS             complement(5471..6088)
                     /locus_tag="VIH70_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            complement(6189..>7098)
                     /locus_tag="VIH70_02955"
     CDS             complement(6189..>7098)
                     /locus_tag="VIH70_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8115352.1"
                     /translation="GTDDADDLRLYLEAAAREPLLTKEEEVELAILIEHGKEGEERLR
                     AGRLRSERSIMKARQEVRKAEAARQRFIMANLRLVVSVARKYQGQGLPLLDLIQEGNI
                     GLMRAVELFDWRRGFKFSTYATWWIRQGITRAIADRSRSIRLPVHIHDRLRKLNRTRF
                     QFAQTMGREPSRDELARALDTTVDEIDDLTEMGRREPLSLQSPVGEDTELGDLIEEVD
                     SDAAFNEVEDALLREEIGTAVDRVLDERERHVVALRYGLENGQPLSLRETGKVLGLSG
                     ERVRLIEREALRKLRDSNLISAVAAM"
BASE COUNT         1069 a         2541 c         2441 g         1047 t
ORIGIN      
        1 gcatcgagat atagagaccg tcctccacct cgccggcgtc gtagatcgtc acgacgttgg
       61 gatgatcgag ccctgcggcg atccgcgatt cgcggatgaa tcgctcacgg aacatctcgt
      121 cctcggccag gtgcggagcc aggatcttca gggcgacccg cctgcccagg cgcaggtgct
      181 cggccagata cacgacgctc gcgccgcctc gaccgatgac ctcctcgatc cggtggcccg
      241 cgaacatcgt gccgatccgg gggtccatcg ccaggttgta gcactccctc cgaacggcga
      301 acagaccgga tccgggggcg acgaaggatc gacgtccctg cgtttgaccg actcggaccg
      361 gggtcccacg attggggcac ccgaccgtcg gagcatcgat gtgatcgttg ggagaacggc
      421 cgagatcgcc tcgatcgcat ccttcctcga cgacgttccg gacgggccga ccgccctcgt
      481 cctcgatggt gagcctggca tcgggaaaac cgtcctgtgg gcacgtgccg tggaggaggc
      541 ccgcggccgg ggctatcggg tcatgagctg caggcccgcg ggacccgatg cgcagctgtc
      601 cttcgtcgcg ctcggcgacc tgttctcggc cgacaccgag ccgctgttcg agaccttgcc
      661 gcaaccccag cgagaggcgc tcgaggttgc cttgctccga cgccatcggg gcggggatgc
      721 accggatcca cgcgcagcgg ccgtcgcgac gctcggggtc gttcgtgcgg ccaccgcgca
      781 ggcgccctcg atcatcgcga tcgacgacgt gcagtggctc gaccccgcga ccgcgcgtgt
      841 gctcgcgttc gcgctgcggc gcctcacggc cgagcggatc ggcgtcgtcg tgagtcgtcc
      901 gccgaccggg gaagcgaccc cgctgggcct cgccgacacg atgcatggcg aggggttccg
      961 aacgctccgc gtcggaccgc tgggcgccca cgagatcggc gtcatcgtgc gagatcgaac
     1021 gggtgtgacg ctgtcgaaac ccgacgtcga gcggttggcc gagctgtgcg gaggcaaccc
     1081 cttctacgcc ctggagatcg ctcgagcccg ggagcgcggc gacgaagggg tgaccgggca
     1141 acgtctcccg attcccaaga gcgtgcacga ggacctcgtt cgagcgcgcg tcgacagcgt
     1201 atccgccacg acgcgggagg tcctcctgtt cgcggcctcg atctcccgcc cgacggtgga
     1261 gttgctgcga cgcgcgatcc cggccaccac cctgaacccc gggctccagg gggcgatcga
     1321 tgcgggtctg atcgaggtcc tcggcgccga cgtccgtttc atccatcccc tgtaccgctc
     1381 ctccatctac gcggatacgt cccgtcgacg ccgtcacgag atgcacgccc ggctcgccga
     1441 gatcacgcct gaccccgagg agcgagcgcg gcatctcgcg ttggcggcgg atggatccga
     1501 cgggaagatc gccgccgagg tcgaggcggc ggcggcgtcg gcccgcggcc gaggggcccc
     1561 cgaggtcgcc gccgatctcg tcgagcacgc gatccggctt acgccgagcg acgacacggc
     1621 ctccctgcgc cggcgcctgc tcgtgtccgc ccaagaccgg atgtccgcgg gtgacgggga
     1681 cggcgcgcgc gaacgcgctc gggaagccct ccgtctctcg gagccggggg tcgctcaggc
     1741 agaaccgctc cgacggatcg gtgccagcga gttcgctcgc ggcgcgctcg cggaggcgag
     1801 ggcgacgctc gccgaggcgc tcgcgcgcgc cggagaggac gaccgtgcga gcgccgagat
     1861 ccatcgcgag ctcgcgatga tcggaatccg ctcgggggag gtcggggccg ccgaacggga
     1921 cgccgcagcc gctctcgaac gggcgggtcg cacgggcgat gcgcagctca ccgaggccat
     1981 cgcgaccgca gcggccgagg tggacctgct cctggggagg tcctcctccg acctctcgca
     2041 ggtcttcaac gggtacgtcg cccccgacgc tcccctgtcg ggatcgccgg agctggtcgc
     2101 ggcactggca gaggcgttcg ctgggcagct cgaccgctcc gagcggcggc tggagcggct
     2161 ccgccgccag gcacacgacc gtggcgacga gcccgggcgc ctgctcgtga cgacgcgact
     2221 tgcggagctc cgaatccgag caggatcgtg ggatcgggcg gcgaccctcg cgcacgaggc
     2281 ctacgagctc gccctcgatc tggacacgag ccacggcctc cagctcggcc tcatggcctc
     2341 cgcggcggca gggagcggtc ggatcgacga cgcacgccgg ttcgccgagc aggggctgac
     2401 gagcgcctcg ggggaccgac ccgcggtgat ctggaacctc gcggcgctgg ggttcctcga
     2461 gttgtcactc gctcgctatg ccgacgcgct gcgcgtgctc gggagggcgg ggggcctcct
     2521 cgccgagacc ggcatcgccg acccgtccgc gttcccgttc ctggcggacg aggccgaggc
     2581 gctcatcggg gtcggcgagc tcgcgaccgc gtcccgccgg atcgccgcgc tcgaggaggc
     2641 gagcgatcgg ctcgatcgag acctgatcag aggtcaggcg gcacgatgca ctggactcgc
     2701 gagcgccgcc gaaggcgaca tccccgcgtc cctccgggag ctggaacgcg ccgtcgagat
     2761 ccacgaacgc ctgggggtcg cgatcgaact cggtcggtcg ctcctcgcac tcggtatgtc
     2821 ccgacgtcgt gaccggcaga agcgacccgc tcgcgaggtc ctcgagcgtg cgctcgaggc
     2881 attcaccacc accgagacgc cgctgtgggc gaaccgagtt cgcgacgaga tcgcgcggat
     2941 tggtggccgg cgtgcatcgg tgggcgagct gaccgagagc gaagcccagg tggcgcgtct
     3001 ggccgccgcc ggactcacga accgcgagat cgcgcggacg ctgtcgatcg ggattcggac
     3061 cgtcgagggt catctctcac acgtctacac gaagctcgag atccgatcgc gcaccgagct
     3121 ggcggcgttc gacctcgacg tgtgatcccg aactccatgt gacgtaggtc accccctccg
     3181 cagagcgcca cctgtcgatc ggcgcggcat gcgtcgcaaa cacgcgtgat acctcgggtt
     3241 tctcggcaac ccgccctccc tagcgtcggc cgcgtgaagc caacgctcac cgagggaggt
     3301 ggaaccgatg ttcagcaagt tcctcagcag ccgggcgtgg ttggtcgcct tcgtgatgtt
     3361 cgcgacggcc ttccccgtcg tcccactcgc cgaaggtgag gtcccgatcc ctcagggatc
     3421 gcgcacgagc ccgccgaaca tcgtgctcat cctcaccgac gaccaacgag cggacacgct
     3481 ccggtacatg ccgatcgtgc agcggttgtt ggtccagacg ggcgttcgct tcgataacgg
     3541 ctacgtggcg aatccactct gctgcccgag ccgagccagc atcctcaccg ggacctactc
     3601 ccatacgaac ggcgtctact cgaaccatgg accccatgga gggttcgccg cgttccacga
     3661 cgcgacaacg atcgcgactg tgctgcaagc ggccgggtat cggaccgggc agttcggcaa
     3721 atacttcaac ggctaccggg acacgaccta cgtgccaccg ggatgggatc gatggttcgc
     3781 cacgtacgat cgggacagcg gctactacgg ctacaccgcc gtgtcggacg gggacgaggt
     3841 gcagtacgga tccgacccgg cggactacgg acagacgatc ctgcgccgcg aggccgtctc
     3901 cttcatcgag gatacggacg cctccaagcc gttgttcatg tactgggcga cccacgcccc
     3961 gcacgccccc gcgatccctg agcggcaaga caggaaggcg ttcgcctcca tcgagcgctg
     4021 gcggtcgccc agctacgacg aagccgacgt cagcgacaag ccatcacacg tccaggccag
     4081 gccgccgatc gacccggcga cggcacggga gatcgatcgg ttccgattgg atcagatccg
     4141 gtccctgcag tccgtggatc gatccgtggg tgcgatcgtc aaggcgctgc gcgagagtgg
     4201 ccggctggag aacacgatca tcgtgttcat gtccgacaac gggatgctgt ggggagaaca
     4261 ccggctccac ggcaagagcg agatctacga ggaggcgatc gccgttccct tcgtcgtgcg
     4321 atacgacgcg atgatcggtc gagcgcgcac cgacgagcac ctcgtgctga acatcgacct
     4381 cgcgccaaca ttcgcggacg cggcgggcgt ggagctgccg aatgccgacg gacggagcct
     4441 cctcccactc ctcgactcat cggacgctgg atggcgcgag tcgttcctcg tcgagcacct
     4501 ccgagcgggg gacaacacga ggtacgcccc cacgttctgc gcggtgcaca ccgaccggta
     4561 cgtcctcgtg cgctacgcga ccggagaaga ggagatgtac gacctcgcac acgatcccgc
     4621 tcagctggag aacgtggccg gctccgcccg gtacgacgag ctgcgcggat cgctccgagg
     4681 tcggctccgc gtgctctgcg atccgctgcc gcccggattc tccttctgaa ctcgatccat
     4741 cacgcaggga cccggtccac aggggccggg tcctcgcgcg agtagcatcc gcggatggct
     4801 cggcgcggcg acctgaacgg atggtggtgg ttcggcatcg ccgtcgtggg cttctcgtgc
     4861 cgcgtcctgt tccgactccg catcgagggt gccgaccggg tcccgtccgt gggccccgcg
     4921 atcgtcgccg gcaaccacgt ctcggcgctc gacggcgtcg tgctggccct gaccacgagc
     4981 tcgcgcagcc ggcgcatgac ccggttcctg gtcgcggcgg agttcttccg caagctctgg
     5041 ttcgggtggg ccctgcgtct gtacggtcag atcccgctgc gccgcggtca gcgcgatcag
     5101 ggcgcgctcg atgtcgcgat cgacacgatc cgtggcggcg cgctggccgg catcttcccg
     5161 gagggaacgg tcaacccgga gccgggggcc gggctgatgc ggggccgcaa gggtgcggct
     5221 cggatcgccc tggccagcgt ggcaaccgtg gtccccgtcg gcatctgggg gacgcaggcc
     5281 cggtggccga gatcgggtct gcacttccgg cgtccgtggc gaccggtcgt cgcgatctcc
     5341 tacggcgaac cgatcccgcc gaagggcgac ccggattcgg cgccggacgt gaaggccttc
     5401 acggacgtca tcctcgacgc gctcgccgcg cagacggagc gggcgaagga gctggccgag
     5461 gcgtagggct tcagccgacg tgatcggcga accacgaggc cgcgagctca gccacgcgct
     5521 cgagcgcccc cggctcctcg aacaggtggg tggccccggg cacgatctcg agccgtgtct
     5581 cggatcggag gcgaccctgc gcccgccggt tgagctcgag tacctggagg tcggcgcccc
     5641 cgacgatcag caacgtcggc gccgtcacgg cctcgagcgc gtcccccgcg agatcgggcc
     5701 ggccgccgcg cgagacgacg gccgcgacgt ccggccggag atgggcggcc gccacgagcg
     5761 cggccgccgc cccggtgctc gccccgaaca aggcagtcgg cagtcggccg acgtcgggct
     5821 caccccgcag ccaggcgacc gccccctcga gccgccgcga gagaagtccg atgtcgaaga
     5881 ccttgtcgcg atcccccgcc tcgtcgtcgt tcaagaggtc gaacaacagg gtcccgaggc
     5941 cctcggcctg cagacgctcc gcgacccgca cgttgcgagg cgatcgcctg ctcgagccgc
     6001 tgccgtgcgc gaacaccacc acgccgatcg ccggttcggt gatcgcgaga tcgccgggca
     6061 ggcccagccc gtcggcctcg acggtcacgg ttcgggaaag gacgtccgcc atcagccgaa
     6121 aagcgggagg gccggcccgc tgtgcgggcc ggccctcccg gcgcccctcg tccgagtcgt
     6181 cgtcggcgtc acatcgccgc gacggcggag atcaggttcg aatctcggag cttgcgcagc
     6241 gcctcgcgct cgatcagtcg aacgcgctcg cccgagagac cgagcacctt gcccgtctcc
     6301 cgcagcgaca gcggctgccc gttctccagg ccgtaccgca gcgccacgac gtgccgctcg
     6361 cgctcgtcga gcacgcggtc gacggccgtc ccgatctcct cacggagcag ggcgtcctcg
     6421 acctcgttga acgcggcgtc cgagtcgacc tcctcgatca ggtcgcccag ctcggtgtcc
     6481 tcgcccacgg gcgactgcag cgacaggggc tcgcggcggc ccatctcggt caggtcgtcg
     6541 atctcgtcga ccgtcgtgtc gagcgccctc gcgagctcgt cgcggctcgg ctcgcggccc
     6601 atcgtctggg cgaactggaa ccgcgtgcgg ttcagctttc gcagccggtc gtggatgtgc
     6661 accggcagcc ggatcgaacg cgagcgatcg gcgatcgccc tcgtgatccc ctgacggatc
     6721 caccacgtcg cgtacgtgga gaacttgaag ccgcggcgcc agtcgaacag ctcgaccgcg
     6781 cgcatcagcc cgatgttgcc ctcctggatc aggtcgagca gcggcagccc ctggccctgg
     6841 tacttccggg ctaccgagac gaccagccgc aggttggcca tgatgaagcg ctgccgggcg
     6901 gcctccgcct tccggacctc ctggcgagcc ttcatgatcg aacgctcgga ccgcagccgg
     6961 ccggcgcgca ggcgctcctc cccctccttg ccatgctcga tcaggatggc gagctcgacc
     7021 tcctcctcct tggtgaggag tggctcgcgc gccgccgcct ccaggtacag ccggaggtcg
     7081 tcggcgtcat ccgtgccg
//
LOCUS       DATBRK010000100        22030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_733423, whole genome shotgun sequence.
ACCESSION   DATBRK010000100 DATBRK010000000
VERSION     DATBRK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733423"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VIH70_02960"
     CDS             complement(<1..240)
                     /locus_tag="VIH70_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115353.1"
                     /translation="MSGRVVRRRTDVLVALAGLALFVPCAVIASDGTVGPVELAVFHA
                     INGLPEALSPVMQWVQWLGVLAVGPMVALGAALFRR"
     gene            complement(237..1301)
                     /locus_tag="VIH70_02965"
     CDS             complement(237..1301)
                     /locus_tag="VIH70_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEY8115354.1"
                     /translation="MAHLVTLIPGDGIGPEVSEATRRVLDATGVPIQWVVEEAGADIL
                     AHEGTTLPGRVLDQIRDSRVAIKGPITTPVGSGFRSVNVELRQALDLFAAVRPSRSLP
                     GLPTRHPDVDLVVIRENTEDLYQGIEFERGTPDALKLREELLRLSGRELREDSGFTVK
                     PISVTGARRIVRFALDFVGAHGRRKVTLGHKANIMRFSDGLFLSIGQIEAEGHPEVWF
                     EEMEIDQLSMRLVREPAEFDVLLLPNLYGDIISDLCAGLVGGLGLAAGANLGWEYAVF
                     EPVHGSAPDIAGRGIANPIAMILSGVMLLRHLHEAAAATAVERAVDRVLERGVARTPD
                     LGGTSSTDAVAAAIEETLRG"
     gene            complement(1362..2141)
                     /locus_tag="VIH70_02970"
     CDS             complement(1362..2141)
                     /locus_tag="VIH70_02970"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019548740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY8115355.1"
                     /translation="MAATGSGGLGEVLEGSDLLAAGSAHTVTLTLRIRRYNPELRGEE
                     SWWDEFEIQADPDDRLLDALHQVKWYHDGTLALRRSCAHGICGSDAMMIDGKNALACK
                     VIVRDVAPKVTVEPIRGLPVLKDLIVDMEPFFAGYRSVLPYLINDSGEPDTERLQSPE
                     ERHRFDDTTKCILCAACTTSCPIFWGDEEYVGPAAIVNAHRFIYDSRDEAGRERLTIL
                     SEKTGVFRCRTTFNCTEACPRGIEVTKAIQEVKRSILFDRF"
     gene            complement(2141..3877)
                     /gene="sdhA"
                     /locus_tag="VIH70_02975"
     CDS             complement(2141..3877)
                     /gene="sdhA"
                     /locus_tag="VIH70_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY8115356.1"
                     /translation="MTIFHTYDAVIVGAGGAGLRAAIETSDRVKTAVISKLYPTRSHT
                     GTAQGGVCAALANVEEDSPEWHAFDTVKGGDYLVDQPAAQLMCEEAVDAIYQLEHWGL
                     PFNRTPEGKIDQRRFGGHTRNHGEAPVRRACFAADRTGHMILQTLYQQCVKRNVRFFN
                     EFYVLDQLMAEGACAGVVAYELATGEIHVFRSKAVLYATGGYGRMFKVSSNAHTLTGD
                     GPGVLFRRGLPLEDMEFFQFHPTGVYKMGILLSEAVRGEGGILLNREGERFMERYAPT
                     VKDLAPRDMVSRAIFQEIKEGRGDGPNRDAVWLDVRHLPRDVIEEKLPDVTEFARVYL
                     GVEPTTELVPIQPTAHYAMGGIPTNVDGQVVVGQDETPIPGLYAAGECACVSVHGANR
                     LGTNSLLDIVVFGRRGGAHAAGFCGDADLPDLPERPEGPVRDMVDGLLNRPEGANAAD
                     IRNELQDSMQELAFVVRTEESLRKMQEILGSLRERFERVYVQDTGAMFNTELMEVVEL
                     GFLLECADALVAAALARDESRGGHYREDHPLRDDANWLKHSLAYRDEDGSIRLEHKPV
                     KLGPYVPMERKY"
     gene            complement(3880..4323)
                     /locus_tag="VIH70_02980"
     CDS             complement(3880..4323)
                     /locus_tag="VIH70_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HEY8115357.1"
                     /translation="MSVEAPERPTPSPRGGSPGIRGRERPVGGFELWSWLFMRISGLV
                     LLILAVGHVLIMHVWDDGVDRVNFGFVAARWQSPFWRTWDWALLVLALIHGINGLRII
                     VQDYVRWPAVRFAVDMAFFVIGFALFVLGTVVVFTFDPSQWPGTV"
     gene            complement(4323..4718)
                     /gene="sdhC"
                     /locus_tag="VIH70_02985"
     CDS             complement(4323..4718)
                     /gene="sdhC"
                     /locus_tag="VIH70_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY8115358.1"
                     /translation="MAKRKSDVGTLYKGSPGQWSWLLHRITGVAVILFLFAHVVDTAV
                     VGWGPEAYNRVVGVYHNWVVKLLELGLVAAVLYHAINGVKIMVFDFWPSSARHMKGIA
                     LASTIVFALSMIPITIIMGHGILDSAGVF"
     gene            complement(4782..5429)
                     /locus_tag="VIH70_02990"
     CDS             complement(4782..5429)
                     /locus_tag="VIH70_02990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEY8115359.1"
                     /translation="MDIVDPRVEDYMRSLLTRYDEPVLLEMEAEGAERNFPIIGRLVG
                     VAVEVLARTVGAERVFELGSGFGYSAYWFARAVGPGGEVHATDGDPANEPKALDYLSR
                     ADLAAPIRWHVGDAVENLGAVEGEFDVVYNDIDKQGYPDAWRAARERIRVGGLYLCDN
                     VLWSGAVADPAGDHGRDAKTTNAILEHNRMIAEDERFASSIVPTRDGVFVALRVA"
     gene            5463..7046
                     /locus_tag="VIH70_02995"
     CDS             5463..7046
                     /locus_tag="VIH70_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8115360.1"
                     /translation="MSDVGPRSTDSGIDLEPVYEPDDLGGFDPERDLGVPGGPPFTRG
                     VYPSMYLGRLWTMRQYAGMATADETNRRFRYLLDHGQTGLSVAFDLPTQMGLDSDHPR
                     AEGEVGKTGVAIDSIEDMGRLLDGIPLDAVSTSMTINATAPILLLLYELVAEERGIAA
                     SALNGTVQNDLLKEYAARGTYIYPPRPSMRLITDLFAYCGQTIPRWNTISVSGYHMRE
                     AGATAVQEIAFTLADGIAYVQAAIDAGLEVDGFAPRVSFFFACHMNFFEEIAKFRAAR
                     RMWATIMAERFGAKDPRSLMLRFHTQTGGATLTAQQPRNNIVRTALEALAAVLGGTQS
                     LHTNSFDEALALPTEQAAKIALRTQQVIGHETGVTHTVDPLGGSYYLESLTNEVESRA
                     WRYVERIDAMGGAVDAIEAGFYQDEIAEAAFRIQQGIESGERVVVGVNRFEDPDEQPV
                     RIQTIGQEEVARQVERLRALRASRDQGAVDRALQEIESAARGTANLLPPMKLALRARA
                     TLGEVSDVLRGVFGEYQPR"
     gene            complement(7075..7242)
                     /locus_tag="VIH70_03000"
     CDS             complement(7075..7242)
                     /locus_tag="VIH70_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115361.1"
                     /translation="MGQSVTYVTELAVGIGCVVAAVATARGHRLRWLALVLAGAGASA
                     IVHAIVELATE"
     gene            7269..7961
                     /gene="npdG"
                     /locus_tag="VIH70_03005"
     CDS             7269..7961
                     /gene="npdG"
                     /locus_tag="VIH70_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEY8115362.1"
                     /translation="MDISIIGGTGDEGFGLTLRLARAGNRVTIGSRIAEKGSAVADTA
                     REMLGPGANVDGAANEEAATADVVIVTVPFGGQAEIYRSIKDHVKTDAIVVDCTSPLA
                     TAVGGRAWQVVRPWHGSALEQAQAILEQVHPVRMVGAFHTISGEALQNLEHEMESDVL
                     VCGTDVEAKAVVGGLVDQIPNLRWVDAGDLTQARTLEPLTALLISINRTYGIHDAGVR
                     ITGRDAWGLPGA"
     gene            complement(7972..9699)
                     /locus_tag="VIH70_03010"
     CDS             complement(7972..9699)
                     /locus_tag="VIH70_03010"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY8115363.1"
                     /translation="MAKPRILIANRGEIAVRIIRTVRELGYTSIAVYSDADRDALHVE
                     MADQAHRIGPALAADSYLSIGAILEAASRAKATMIHPGYGFLSERAHFASAVADSGFT
                     FIGPPAEAIEMMGDKAAARRAADRVGVPIVPGTPDPVTPDQAPAEAERIGFPVLVKAA
                     FGGGGKGMQVVRKPEELESALARATREAQAYFGRPDVFLERYIDRAHHVEAQVFADTR
                     GNVFFLGERDCTVQRRHQKLIEETPSPVVDADLRSRIGEAAMALAKEAGYVNAGTVEC
                     ILDEDGSFYFLEMNTRLQVEHTVTEMATGLDLVALQIRVALGEKLELSDVRPTGHAIQ
                     CRINAEDPARNFLPGPGRVIAYREPSGPFVRVDSAVRAGREIPADYDSMFAKVIVSAE
                     NRVRSIARMDRALSEFHVEGVPTTIPLLRWVLGTRAFSKSTHSTTWLEKALRDAELPE
                     AGTPAPPPSDTRQPVPAELLLEVDGRRVPVRIFDQRRDTAPKVPSRHSAHGGDHVHSV
                     IAAPMQGTILRVLVEPGQEIQAGDVVCILEAMKMENAIPAPREGVVTEIPVHAGQNVQ
                     PGDPLVELD"
     gene            9808..10560
                     /locus_tag="VIH70_03015"
     CDS             9808..10560
                     /locus_tag="VIH70_03015"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY8115364.1"
                     /translation="MLSEDALVRALERVGLRAPVRFDEVTASTQATALELATAGTPEW
                     TLVAAGHQTDGRGRLGRMWVDLPGRALMFSFVLRPGLDPGSGGLLTLLAGTAMAQACF
                     ELADQRAACKWPNDLLVAGRKSGGILAESRLVGDRFEYVVIGIGVNLGAPPSDVSEAG
                     AVEAEDDELLGTFLAAFVRGYEPEHPAFAESVIAAYRQVCATLGTRVRASTTAGAIVE
                     GEAVDLDERGGLLVRTTGGDEVVRFGEVEHLE"
     gene            10570..11262
                     /locus_tag="VIH70_03020"
     CDS             10570..11262
                     /locus_tag="VIH70_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY8115365.1"
                     /translation="MPFPRRLLIENEQLVLELRPHWIALVMPAIVTILVIVGWSLAVS
                     YGPDSGTGRDVTFWGATAIAILVLIWFPLRRLVAWATSYFVVTSDRVIHREGWIAKRS
                     MEIPLEAINDVRFHQRVFERMIGAGDLIISSASEFGREVFGDIRNPEHVQRTIYQQGE
                     LNKDRMYRGLGRDSAPAVPAPPATSGGTTPAMPPASASTTGELERLAELRNRGVLTED
                     EFQSQKRKILGG"
     gene            11296..11541
                     /locus_tag="VIH70_03025"
     CDS             11296..11541
                     /locus_tag="VIH70_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115366.1"
                     /translation="MDTLNRSIARTPPATKIELVGYVLVAVGYVVITLGILEIGGLFN
                     IGIGFAVAWAGCVLMFLGEERREFDEEAEFRRELEEL"
     gene            11593..12762
                     /locus_tag="VIH70_03030"
     CDS             11593..12762
                     /locus_tag="VIH70_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEY8115367.1"
                     /translation="MEPRVAVVGVGHAGFAPLTAGVSYKELMFEAAQRAYHDAGVDPR
                     TEIDSFVCCSEDFLEGTSIFDEYVPDQLGAMQRPVHTVAADGLFGVSTGWMLIRSGLA
                     SIVAVEAHSKVSDVRTLGAIEAFALDPVLNRPLRFPTLAIAGLDMNAFLERTGLSEEH
                     CSIVAAKNRSNALDNPRAAYPADVTADDVDDSSPVAWPLKALEVAERADGCIVVVLAA
                     EDRARELTEGPVWLLGTGWSSGSPSLESRTWGETEPVTRAAESAYRRAEIDDPQEEID
                     LAEVDDTFAYKELLHLEALGLGQAPELAQLVEEGGLEPGGDLPVNVSGGSLGQGNLFE
                     ANGLVRLLECVEQLRGEAGERQVDEAYVAVAQSARSAPSTSAAVLVLANDEGAGR"
     gene            12759..13124
                     /locus_tag="VIH70_03035"
     CDS             12759..13124
                     /locus_tag="VIH70_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8115368.1"
                     /translation="MKLRALDHVWFWVTDLDRSVRFYEEQLGLGLRVRHGDEWAELEA
                     GAIHIGLHGARGEGNVPHGGTAVFRVDDLDLAKTALEERGVDFHEHLGEVPGIARYAS
                     FDDPDGNSMQLIEYIPGEG"
     gene            13125..14279
                     /locus_tag="VIH70_03040"
                     /pseudo
     CDS             13125..14279
                     /locus_tag="VIH70_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010923764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
     gene            14277..14882
                     /locus_tag="VIH70_03045"
     CDS             14277..14882
                     /locus_tag="VIH70_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEY8115369.1"
                     /translation="MGREGPPATATQLETPPTATPATSLSDAEFRSATGAVEDEIGAR
                     YAWDAGVAIGRFLDGLRDGRIMGRECRNCERVLVPPRMFCERCFRGTDAWKQVEHTGV
                     VQTFSICNVRWDMQPLDEPELPAVIAIDGSDGGFLHTLGEVERDEIRIGMKVEAVWKP
                     VSERTGSILDIAYFRPRQGDPGSSTSERDRASGSRLGGDAS"
     gene            14882..15352
                     /locus_tag="VIH70_03050"
     CDS             14882..15352
                     /locus_tag="VIH70_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY8115370.1"
                     /translation="MLEKIARPEEIRRWEGDLPVRHRYTPGVAGDAFFTALKERGVLL
                     GSRCESCAYTYVPARLFCERCFSELSADTEVGPAGELVSFTIVFQGIEGEPLDRPQTL
                     GAVRLDGADAVLIHRVEPGEEPLEIGERVEVVVEPESRRTGSILDIAGFRLVET"
     gene            complement(15397..15870)
                     /locus_tag="VIH70_03055"
     CDS             complement(15397..15870)
                     /locus_tag="VIH70_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8115371.1"
                     /translation="MAMAEKTTAKKGTTTSAKRTGAGNRFTAEERAAMKERARELKAA
                     KTKEEGERALLAKIAEMPEPDRAMAERLHAIITKSAPSLSPKTWYGMPAYAKDDEIVC
                     FFKSADKFKSRYATFGFEEAANLDEGAMWPTSFALKRLTAADEKKIGALVKKAAS"
     gene            complement(15932..16342)
                     /locus_tag="VIH70_03060"
     CDS             complement(15932..16342)
                     /locus_tag="VIH70_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8115372.1"
                     /translation="MDITIYQTLLPHEDPDASRAFYRDTLGFEVRNEVEYGGMYWITV
                     GPPDQPGTSIVLYPPAATPGITDDERRTIAEMMAKGTFASINLATKDVDGAFERLQAG
                     DAEVVQEPTDQPYGVRDCAFRDPAGNLIRIQELR"
     gene            complement(16348..16797)
                     /locus_tag="VIH70_03065"
     CDS             complement(16348..16797)
                     /locus_tag="VIH70_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8115373.1"
                     /translation="MTSRSASAQHLRDLAVLRRVRDRIDREYAQPLDVEALARGAHMS
                     AGHLSRQFRLAFGESPYSYLMTRRIERAMALLRRGDLSVTEVCFDVGCSSLGTFSTRF
                     TELVGVPPSAYRRQSPRAMAGMPPCVAKQVTRPIRNREAPLAEPHVA"
     gene            16882..20037
                     /locus_tag="VIH70_03070"
     CDS             16882..20037
                     /locus_tag="VIH70_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8115374.1"
                     /translation="MATLTCERCGLPLPDEARFCPNCGYPVGAPAPEERKFVTVIFVD
                     LVGSTQLSSQIDPERYREVLTRYYRMVAQELESLRGRAYNFAGDAVVGVFGLQHTHDD
                     DALRAVRAGLGLVDRVGRLGEEIGLPVPLRIRVGIHTGPVAIGSEASEQGLLYGATVN
                     MAARLQQAADPGTVLVSETSYLLSLSQVQYGPMREVEAKGFEDEARAWPVAALQPGSG
                     RRTIPLVNRKRELRLLQDAFDGARETRRGHLVTLFGEPGIGKSRVVDEFVAGLPDDAK
                     VLMGRASPFEEDVTFGPLAQILLHEMGEAAHAPPEALRTRLEEIVVESTPPDEAKQIV
                     ERLCIALGIAGEARDEKRYRVAEIRSGLLALLRGFSSRGPVVVVLEDLQAAQAPMLEL
                     VEELVREADGISLLVICAARYTLLDDRPGWGGGRGNSVNLYLETLSLDDSTQLARDAG
                     EGLDEATAQRIAQHAGGNPFFIIETTGMLLHQGTGLSADTGPLPPALLPPTVQAVIAA
                     RIDHLSPQGRDLIRKASVFARSSFTLAELDLIAVPTGEALGRLEEEELLEHDGDRPDV
                     WRFSHGMVRDVAYESLPKRERQRLHLLVADGLSGDPETAARRPRAIAFHLEQAALAAL
                     DLNPRDRTLAERAVDALARAGDIAREGPNMKAAEEDYRRALELAGPERGWGLREARIL
                     ASLGEIRYWLGEFEPAVPVLERALKLGDGDPAVRAQASRFLGDIELSILGDAKRAAEL
                     LDDALEAARGLGDPWTLARTLLVAAWAPYFRQDAEGARSMFREALETARANPEGDRWS
                     EARSLVGLSTLESDEGDEEGSLRLAAEALVVAETSGDRFSIAVAHEAVGGTMRRMMRL
                     EDAEPNLDAAVDAFRELGARWELASALTSRGIARRLAGRADDAVKDLREAYRLCRELK
                     ERSIITWTASALARAFVNAGDPGSARRVLDEVDSVANVHTAAGEDWIDYADVEILLAE
                     GERDAALERAISILRYERDEGSEKDIAARVWWISQVFGVEAAGGEGEVERARELLERT
                     QMLQAFREAELVTSRSAISRA"
     gene            20056..21213
                     /locus_tag="VIH70_03075"
     CDS             20056..21213
                     /locus_tag="VIH70_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8115375.1"
                     /translation="MDFDLTPEQDELRAAVRAFAQEIVAPAAAEADEREEFPTEIVKR
                     AAELGLFGIPFPEEVGGQGGDLLSFCLCLEEIGRYDSSLAITLEAAVGLAANPLFRFG
                     TDEQRQRWLVSMARGEAIGAFALTEPGGGSDARAIRTTARFDGDGWVIDGSKAFITNS
                     GTPLTSVIIVAALDETADDGDVSTIVVPVGTPGLTVGASYRKVGWRASDTHAVSFDAC
                     RVPADHLLGERGRGYAQFLETLADGRVAIAALSVGLARGCLEESVRYAAEREAFGQPI
                     GAYQAVQFKIADMRVGVETARLAYQRAAWLRDRGRPFATEASIAKLYASEVAVSSARE
                     AVQVHGGYGFVEDFPVARFYRDAKVLEIGEGTSEIQRVLIARALGLPGTDL"
     gene            21344..>22030
                     /locus_tag="VIH70_03080"
     CDS             21344..>22030
                     /locus_tag="VIH70_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115376.1"
                     /translation="MAENHSGSGVVFRRALAVVVALALLTSFAQVALAEPSTQEKLDA
                     AKAEFEQLKNQIASQQQVLNRLSAEAAVIAERWDVASGRWEQVTSELRKTQLALAEAQ
                     AEYQGLKSDLEERARQAYITGPGSGLEFLLGASSIADLSARMEYVNALSQEDADLATE
                     VQNVRNTLAAQKEDQEKLQAKAAKALKKVESEQAALDAKFDEQQAVLDDLDAKKARAE
                     DLVKRLTKRQQ"
BASE COUNT         3265 a         7582 c         7942 g         3241 t
ORIGIN      
        1 ccgacggaac agggccgccc cgagggcgac catcggaccg accgcgagga cgcccagcca
       61 ctgcacccac tgcatcaccg gcgacagcgc ctcgggcagc ccgttgatcg cgtggaacac
      121 cgcgagctcc acgggcccga ccgtgccgtc cgaggcgatg accgcgcacg ggacgaacag
      181 cgcgagcccc gcaagcgcga cgaggacgtc ggtccttcgg cgaacgacgc gcccgctcat
      241 ccgcgcagcg tctcctcgat cgccgcggcg accgcgtcgg tcgacgaagt gccgcccagg
      301 tcgggcgtgc gcgcgacacc gcgttcgagc acccggtcga ccgcgcgttc gacggcggtc
      361 gccgcggcgg cctcgtgcag gtggcggagc agcatcactc ccgacaggat catcgcgatc
      421 gggttcgcga tgcccctgcc ggcgatgtcc ggggcgctgc cgtgcacggg ctcgaacacc
      481 gcgtactccc atccgaggtt cgcgcccgcc gcgagcccca gcccgccgac cagcccggcg
      541 cacaggtccg agatgatgtc gccgtacagg ttcggcagca acaggacatc gaattcggcg
      601 ggctctcgca ccagccgcat cgagagctga tcgatctcca tctcctcgaa ccagacctcc
      661 ggatgtccct cggcctcgat ctgcccgatc gacaggaaca acccgtcgga gaaccgcatg
      721 atgttcgcct tgtgcccgag cgtgaccttg cgccgtccgt gcgcccccac gaaatcgagc
      781 gcgaaccgga cgatccgccg cgcaccggtc accgagatcg gcttcaccgt gaacccggag
      841 tcttcgcgca gctctcgccc cgaaagccgc aggagctctt cgcggagctt cagggcatcg
      901 ggcgttcctc gctcgaattc gatgccctgg tacaggtcct cggtgttctc gcggatcacg
      961 acgaggtcga cgtccgggtg ccgcgtcggc agccccggga gcgaacgcga gggccgcacc
     1021 gccgcgaaca ggtcgagcgc ctgccggagc tcgacgttca cgctccggaa ccccgacccg
     1081 accggcgtgg tgatcgggcc cttgatcgcc acgcgcgagt cgcggatctg gtcgaggacc
     1141 cgtccgggga gggtggtgcc ctcgtgcgcc aggatgtcgg cgcccgcctc ctcgaccacc
     1201 cattggatcg ggacgcccgt ggcgtcgagc acgcgccggg tcgcctcgga gacctcgggc
     1261 ccgatgccgt cgccggggat cagggtcacc aggtgcgcca cggcccgaac cttatcgggc
     1321 gttcggcgtc gggcgagatc agggatgtgg cggcgggtgt gtcagaagcg gtcgaacaag
     1381 atcgagcgct tcacctcctg gatcgccttc gtgacctcga tgcccctggg gcacgcctcc
     1441 gtgcagttga acgtcgttcg acaacggaac acgccggtct tctccgacag gatcgtcaga
     1501 cgctccctgc cggcctcgtc ccgcgagtcg tagatgaacc ggtgggcgtt cacgatcgcc
     1561 gccggcccga cgtactcctc gtcgccccag aagatggggc acgaggtcgt acacgcggcg
     1621 cacaggatgc acttggtcgt gtcgtcgaac cggtggcgct cctcggggga ctggaggcgc
     1681 tcggtgtcgg gctcgcccga gtcgttgatc aggtacggca ggaccgaacg gtagccggcg
     1741 aagaacggct ccatgtcgac gatgaggtcc ttgaggaccg gcaggccgcg gatcggctcg
     1801 accgtcacct tcggtgccac gtcgcgaacg atgaccttgc acgcgagcgc gttcttgccg
     1861 tcgatcatca tcgcgtccga gccgcagatg ccgtgggcgc acgaccgccg gagtgcgagc
     1921 gttccgtcgt ggtaccactt gacctggtgc agcgcgtcga gcagccggtc gtcggggtcg
     1981 gcctggatct cgaactcgtc ccaccacgac tcctcgccgc gaagctccgg gttgtagcgg
     2041 cggatcctga gcgtgagcgt gaccgtgtgc gccgagccgg cggcgaggag gtccgagccc
     2101 tcgaggacct cccccagtcc tccggatccg gtggcggcca tcagtacttc cgttccatgg
     2161 gcacgtaggg tcccagcttg accggcttgt gctcgagccg gatcgaaccg tcctcgtcac
     2221 gatacgcgag tgagtgcttg agccagttcg cgtcgtcgcg cagcgggtgg tcctcccggt
     2281 agtggccccc gcggctctcg tcgcgggcaa gggccgccgc caccagcgcg tcagcgcact
     2341 cgagcaggaa cccgagctcg acgacctcca tcagctcggt gttgaacatc gcgccggtgt
     2401 cctgcacgta gacgcgctcg aaccgctcgc gcaggctccc caggatctcc tgcatcttcc
     2461 gcaggctctc ctcggtcctg accacgaacg cgagttcctg catcgagtcc tggagctcgt
     2521 tccggatgtc cgccgcgttg gcgccctcgg ggcggttcag caggccgtcg accatgtcgc
     2581 gaaccgggcc ctcggggcgt tcgggaagat cgggcaggtc ggcgtccccg cagaacccgg
     2641 cggcgtgcgc gccgccgcgg cggccgaaca cgacgatgtc gagcagggag ttcgttccca
     2701 gcctgttcgc gccgtgcacc gagacgcacg cacactcgcc cgcggcgtac aggccgggga
     2761 taggtgtctc gtcctgcccg acgacgacct gcccgtcgac gttggtgggg atgccgccca
     2821 tcgcgtagtg cgcggtcggc tggatcggta cgagctcggt tgtcggctcg acgccgaggt
     2881 acacgcgggc gaactcggtc acgtcgggca gcttctcctc gatcacatct cgcggaaggt
     2941 gtcgcacgtc gagccacacg gcgtcgcggt tggggccgtc gccgcggccc tccttgatct
     3001 cctggaagat cgcgcgactc accatgtcgc gcggggcgag gtccttgacc gtgggagcgt
     3061 atcgctccat gaagcgctcg ccctcgcggt tcagcaggat cccgccctcg cctcggaccg
     3121 cctccgagag caggatgccc atcttgtaca cgccggtcgg atggaactgg aagaactcca
     3181 tgtcctcgag cggcagcccc cggcggaaca gcaccccggg gccgtcgccg gtcagcgtgt
     3241 gcgcgttcga ggagaccttg aacatccgtc cgtacccgcc ggtcgcgtat aggacggcct
     3301 tggagcggaa cacgtggatc tcgcccgtcg cgagctcgta cgcgacgacc cccgcgcacg
     3361 cgccctcggc catcagctgg tcgagcacgt agaactcgtt gaagaaccgc acgttgcgct
     3421 tcacgcactg ctggtacagc gtctgcagga tcatgtgacc cgtccggtcg gccgcgaagc
     3481 acgcgcgtct gacgggggcc tccccgtggt tgcgcgtgtg cccgccgaac cgccgctggt
     3541 cgatcttgcc ctccggcgtt cggttgaatg gcaggcccca gtgctcgagc tgatagatcg
     3601 cgtcgacggc ctcctcgcac atcagctgcg cggcgggctg gtcgaccagg tagtccccgc
     3661 ccttgaccgt gtcgaacgcg tgccattcgg gcgagtcctc ctcgacgttc gcgagcgcgg
     3721 cgcagacgcc tccctgcgcg gtcccggtgt gcgagcgcgt cgggtacagc ttcgatataa
     3781 ctgcggtctt cacccggtcc gacgtctcga tcgccgcacg cagcccggcg ccgccggcgc
     3841 cgacgatcac cgcgtcgtac gtgtggaaga tcgtcatggc tagacggtcc ccggccactg
     3901 gctcgggtcg aacgtgaaca cgacgaccgt gccgaggacg aacagcgcga acccgatcac
     3961 gaagaaggcc atgtcgacgg cgaaccggac ggcgggccac cggacgtagt cctggacgat
     4021 gatgcgcaac ccgttgatcc cgtggatcag cgcgaggacc aggagcgccc agtcccacgt
     4081 ccgccagaac gggctctgcc accgcgcggc gacgaagccg aagttcacgc ggtccacccc
     4141 gtcgtcccac acgtgcatga tcaggacgtg cccgacggcg aggatcagca gcacgagccc
     4201 gctgatccgc atgaacagcc aggaccacag ctcgaagcca ccgacggggc gctcgcggcc
     4261 tcggatcccc ggggacccgc cgcgtgggga cggcgtcggg cgttcgggtg cctcgacgga
     4321 catcagaaga cccccgcgga gtccaggatc ccgtggccca tgatgatcgt gatcgggatc
     4381 atgctgaggg cgaagacgat cgtcgaggcg agcgcgatcc ccttcatgtg acgtgcgctc
     4441 gacggccaga agtcgaagac catgatcttt acgccgttga tcgcgtggta cagcaccgcg
     4501 gcgaccagcc cgagctcgag cagcttgacc acccagttgt ggtacacgcc gacgacgcgg
     4561 ttgtaggcct ccggacccca gccgaccacg gcggtgtcca cgacgtgcgc gaacaggaac
     4621 aggatcaccg cgacgccggt gatgcggtgc agcagccagg accactgtcc cggactgccc
     4681 ttgtacaggg tcccgacgtc ggacttccgc ttcgccatca gccctcgaac acctagccta
     4741 gacgcggctc cgccgaacgc ctgcccggag ccgcgcggag tctacgccac gcggagggcg
     4801 acgaacacgc cgtcgcgggt cgggacgatc gaggaggcga agcgctcgtc ctccgcgatc
     4861 atgcggttgt gctcgaggat cgcgttggtg gtcttcgcgt cccgaccgtg gtcgcccgcc
     4921 gggtcggcga ccgcgcccga ccacagcacg ttgtcgcaga ggtagaggcc gccgacgcgg
     4981 atccgttcgc gagcggctcg ccacgcgtcg gggtacccct gcttgtcgat gtcgttgtag
     5041 acgacgtcga actcgccctc gacggcgccg aggttctcga ccgcgtcgcc gacgtgccag
     5101 cggatcgggg ccgccaggtc cgcgcgcgag aggtagtcga gcgccttcgg ctcgttcgcc
     5161 gggtcgccgt ccgtcgcgtg gacctcgccg ccggggccca ccgcccgcgc gaaccagtac
     5221 gccgagtacc cgaagcccga cccgagctcg aatacccgct ccgcgccgac cgtacgcgcg
     5281 agcacttcga ccgcgacccc gaccaggcgg ccgatgatcg ggaagttgcg ctccgcgccc
     5341 tcggcctcca tctcgagcag gacgggttcg tcgtagcggg tgagcagcga gcgcatgtag
     5401 tcctcgacgc ggggatcgac gatgtccacc gtcccgatgc taccggctac cctgggtccg
     5461 cgatgagcga cgtcggacca cggtcgacgg actccgggat cgatctcgag cccgtctacg
     5521 agccggacga tctcggcggt ttcgaccccg aacgggacct cggcgtcccc ggcggaccgc
     5581 cgttcacccg cggcgtgtac ccatcgatgt acctcggccg cctgtggacg atgcgccagt
     5641 acgccgggat ggccacggcc gacgagacca accggcgctt ccgatacctg ctcgatcacg
     5701 ggcagacggg gctctcggtc gcgttcgacc tgccgacgca gatgggattg gactcggacc
     5761 accctcgggc cgagggcgag gtgggaaaga ccggcgtcgc gatcgactcg atcgaggaca
     5821 tgggccggtt gctggacggg atcccgctgg acgcggtctc gacgtcgatg acgatcaacg
     5881 ccaccgcgcc gatcctgctc ctgctgtacg agctggtggc cgaagagcgg gggatcgcgg
     5941 cgtcggccct gaacgggacg gtgcagaacg acctgctgaa ggagtacgcc gcccgcggga
     6001 cgtacatcta cccgccgagg ccctcgatgc gcctgatcac cgatctgttc gcgtactgcg
     6061 gccagacgat cccacggtgg aacacgatct cggtgagcgg ctaccacatg cgcgaggccg
     6121 gcgcgaccgc ggtgcaggag atcgcgttca cgctcgcgga cggcatcgcg tatgtccagg
     6181 cggcgatcga cgcggggctg gaggtggacg gcttcgcgcc gcgggtctcg ttcttcttcg
     6241 cgtgccacat gaacttcttc gaggagatcg ccaagttccg ggccgcccgc cggatgtggg
     6301 ccacgatcat ggccgagcgg ttcggcgcga aggacccgcg gagcctgatg ctccgattcc
     6361 acacgcagac gggcggcgcg accctcaccg cgcagcagcc gcggaacaac atcgtgcgca
     6421 ccgcgctcga ggcgctcgcg gcggtcctcg gcgggacgca gtcgctgcac acgaactcgt
     6481 tcgacgaggc cctcgcgctg ccgacggagc aggccgcgaa gatcgccctt cggacccagc
     6541 aggtgatagg gcacgagacg ggggtgacgc acaccgtgga tcccctcggg ggctcgtact
     6601 acctcgagtc gctcacgaac gaggtcgagt cccgcgcgtg gaggtacgtg gagcgcatcg
     6661 acgcgatggg aggcgcggtc gacgcgatcg aggccgggtt ctaccaggac gagatcgccg
     6721 aggccgcgtt ccggatccag caggggatcg agtccggcga acgcgtggtc gtcggggtga
     6781 accggttcga ggatcccgac gagcaaccgg tccggatcca aacgatcggc caggaggagg
     6841 tcgcccggca ggtcgagcgg ctccgagcgc tccgggcgtc gcgcgatcag ggtgcggtgg
     6901 accgggcgct ccaggagatc gagtcggcgg cccgcggtac cgccaacctg ctcccaccca
     6961 tgaagctcgc cctgcgcgcg cgggcgacgc tcggcgaggt ctccgacgtg ctccgcggcg
     7021 tgttcggcga gtaccagcct cgctagcggc ccgagactcc tgtccgcggc ttcgtcactc
     7081 ggtcgcgagc tcgacgatcg cgtgcacgat cgctgaggcg ccggcccccg cgaggacgag
     7141 cgcgagccac cgcaggcgat ggccgcgagc cgtcgcgacg gccgcgacca cgcagccgat
     7201 cccgaccgcg agctcggtaa cgtaggtgac ggattggccc atcgcggtgc agggtaggct
     7261 ctgccgccat ggacatctcg atcatcgggg ggacgggcga cgaggggttc ggcctcacgc
     7321 tccggctcgc cagggcgggc aaccgggtca cgatcggctc gcggatcgcc gagaagggtt
     7381 cggccgtcgc ggacacggcc cgcgagatgc tcggtccagg cgcgaacgtc gacggcgcgg
     7441 ccaacgagga ggcggcgacc gcggacgtcg tcatcgtgac cgtgccgttc ggcggccagg
     7501 ccgagatcta ccgttcgatc aaggaccacg tgaagacgga cgcgatcgtc gtcgactgca
     7561 cgagtccgct cgcgaccgcg gtgggcgggc gcgcgtggca ggtggtccgg ccgtggcacg
     7621 gctcggccct cgagcaggcc caagccatcc tggagcaggt gcaccccgtt cggatggtgg
     7681 gcgcgttcca cacgatctcg ggggaggcgc tgcagaacct cgagcatgag atggagagcg
     7741 acgtcctggt ctgcggaacc gacgtggagg ccaaggcggt cgtcggcggc ttggtcgacc
     7801 agatcccgaa cctgcgctgg gtggacgccg gcgacctcac gcaggcgcgg acgttagagc
     7861 cgctcaccgc gctgttgatc tcgatcaatc ggacgtacgg gatccacgac gccggcgttc
     7921 ggatcaccgg tcgcgatgca tggggcctcc ccggcgccta gcgggcgacg gtcagtccag
     7981 ctcgacgagc gggtccccgg gctggacgtt ctgcccagca tgcacaggga tctcggtgac
     8041 gacgccctcg cgcggggccg ggatcgcgtt ctccatcttc atcgcctcga ggatgcagac
     8101 gacgtcgccc gcctggatct cctgacccgg ctcgacgagc acccgcagga tcgtgccctg
     8161 catcggcgca gcgatgacgc tgtgcacgtg gtcgccgccg tgggcggagt gacgcgaggg
     8221 caccttcggg gcggtatcgc gccgctggtc gaagatccga acgggcaccc ggcgcccgtc
     8281 cacctcgagc aggagctcgg ccgggaccgg ctgccgggtg tcggacggcg ggggagccgg
     8341 cgtgccggcc tcggggagct cggcgtcgcg gagcgccttc tcgagccacg tcgtcgagtg
     8401 cgtcgacttg gagaacgcgc gcgtgccgag gacccaccgg agcagcggga tcgtcgtcgg
     8461 gacgccctcg acgtggaact cggacagcgc gcgatccatc ctcgcgatcg accggacgcg
     8521 gttctccgcc gagacgatga ccttggcgaa catcgagtcg tagtcggccg ggatctcccg
     8581 ccccgcgcgc acggcggagt ccaccctcac gaacggcccg gacggctcgc gatacgcgat
     8641 gacgcggccc ggccccggca ggaaattgcg ggccgggtcc tccgcgttga tccggcactg
     8701 gatcgcgtgt cccgtcggcc tcacgtccga gagctcgagc ttctcgccga gcgcgacgcg
     8761 gatctggagg gccaccaggt cgaggccggt cgccatctcg gtcaccgtgt gctccacctg
     8821 cagccgcgtg ttcatctcca ggaagtagaa gctcccgtcc tcgtcgagga tgcattcgac
     8881 ggtgccggcg ttcacgtagc cggcctcctt cgcgagcgcc atcgcggcct cgccgatccg
     8941 cgaccgcagg tcggcgtcga ccaccgggga aggcgtctcc tcgatcagct tctggtggcg
     9001 tcgctggacc gtgcagtcgc gttcgccgag gaagaacacg ttccctctgg tgtccgcgaa
     9061 cacctgcgcc tcgacgtggt gcgcgcggtc gatgtaccgc tcgaggaaca catccgggcg
     9121 gccgaagtac gcctgcgcct cccgcgtcgc tcgagcgagt gcgctctcga gctcctcggg
     9181 ctttcgcacg acctgcatgc ccttgcctcc gccgccgaac gcggccttca cgaggacggg
     9241 gaacccgatc cgctcggcct cggccggcgc ctggtcgggc gtgaccgggt cgggcgtgcc
     9301 ggggacgatc ggtacgccga cgcggtcggc cgcccgtcgg gcggccgcct tgtcgcccat
     9361 catctcgatc gcctctgccg ggggcccgat gaacgtgaag ccggagtcgg cgaccgcgct
     9421 cgcgaagtgc gcccgctcgg acaggaaccc gtagcccggg tggatcatcg tcgccttcgc
     9481 ccgcgacgcc gcttccagga tcgcgccgat cgacaggtag gagtcggcgg ccagcgcggg
     9541 gccgatccgg tgggcctggt cggccatctc gacgtgcagc gcgtcgcggt cggcgtcgct
     9601 gtaaacggcg atcgatgtat agccgagctc tcggaccgtt cggatgatgc ggaccgcgat
     9661 ctcgccgcgg ttcgcgatca ggatccgagg tttcgccacg cgctccgctc cgtcatgctt
     9721 gctcgatcgg accgtcggat cccgatggtc cgcggaaccg gtacccacgg aacaatcggg
     9781 tacccccgaa cgggagtcaa tcgtctggtg ctgtccgaag acgccctggt ccgggcgctc
     9841 gaacgcgtag gtctgcgtgc acccgtgcgg ttcgacgagg ttactgcctc gacccaggca
     9901 acggccctcg agctggcgac cgcaggcacg cccgagtgga ccctcgtcgc agccggccat
     9961 cagaccgacg gtcgtggccg gctggggcgc atgtgggtcg acctgcccgg ccgggcgctc
    10021 atgttctcgt tcgtgctgcg accggggctc gatcccggtt cgggggggct gctcacgctc
    10081 ctcgcgggca ccgcgatggc gcaggcatgc ttcgagctcg ccgatcagcg cgccgcatgc
    10141 aagtggccga acgacctcct ggtggcgggt cgcaagtccg gagggatcct ggcggagtcc
    10201 cgcttggtcg gcgaccggtt cgagtacgtc gtgatcggca tcggcgtgaa cctcggcgcc
    10261 ccgccctcgg acgtctccga ggccggagcc gtcgaggccg aagacgacga gctgctcggg
    10321 accttcctgg ccgcgttcgt tcgcgggtac gagcccgagc acccggcgtt cgcggagagc
    10381 gtgatcgccg cctaccggca ggtgtgcgcc acgctgggga cccgcgtccg ggccagcacg
    10441 acggccgggg cgatcgtcga gggggaggcc gtggacctgg acgaacgcgg cggcctgctc
    10501 gtgcgaacga cggggggcga cgaggtcgtg cggttcgggg aggtcgagca cctcgagtag
    10561 gatgcgctcg tgccgttccc ccgcaggctc ctgatcgaga acgagcagct ggtcctcgag
    10621 ctgcggccgc actggatcgc cctggtgatg ccggcgatcg tcacgatcct cgtgatcgtc
    10681 gggtggtccc tcgcggtgtc gtacggcccc gacagcggca ccggccgcga cgtcacgttc
    10741 tggggcgcga ccgcgatcgc gatcctcgtc ctgatctggt tcccgttgcg cagattggtg
    10801 gcgtgggcga cgtcgtactt cgtcgtgacg tccgaccgag tcatccatcg cgaggggtgg
    10861 atcgcgaagc gctcgatgga gatcccgctc gaggcgatca acgacgtgcg cttccaccag
    10921 cgggtgttcg agcggatgat cggcgccggc gacctcatca tcagctcggc cagcgagttc
    10981 ggacgcgagg tgttcggcga catccgcaac cccgagcacg tccagaggac gatctaccag
    11041 cagggggagc tcaacaagga ccggatgtac cgagggctcg ggcgcgactc ggcacccgcg
    11101 gtgcccgccc ccccggccac atcgggtggg acgacgcccg ccatgccgcc ggcctcggcc
    11161 tccaccacgg gcgagctcga gcgcctggcc gagctgcgga acaggggcgt gctcacggag
    11221 gacgagttcc agagccagaa gaggaagatc ctcggggggt agactcgggg atccgttcga
    11281 cagcgagacg acggcatgga cacactgaac cgatcgatag cgcggacacc gccggcgacg
    11341 aagatcgagc tcgtcgggta cgtgctggtg gccgtcggct acgtcgtgat cacgctcggg
    11401 atcctcgaga tcggcggctt gttcaacatc gggatcgggt tcgccgtcgc gtgggcgggc
    11461 tgcgtgctga tgttcctcgg cgaggagcgt cgcgagttcg acgaggaggc cgagttccgc
    11521 agggagctcg aggagctctg aggctcccgc gccggcgagc ggggtcggcg gcccgatggg
    11581 acaatcgcct tcgtggagcc gagggtcgcc gtggtcggcg ttggacacgc ggggttcgcg
    11641 cccctcaccg ccggggtctc ctacaaggag ctgatgttcg aggcggcgca gcgcgcctac
    11701 cacgacgccg gcgtggatcc ccggaccgag atcgactcgt tcgtctgctg ctcggaggac
    11761 ttcctcgagg gcacgtcgat cttcgacgag tacgtgcccg accagctcgg cgcgatgcag
    11821 cgaccggtcc acacggtcgc cgcggacggg ctgttcggcg tatcgacggg gtggatgctg
    11881 atccgttcgg gcctcgcgtc gatcgtcgcc gtcgaggcgc atagcaaggt ctcggacgtc
    11941 cgaacgctcg gcgcgatcga ggcgttcgcg ctcgacccgg tgctgaaccg cccgctgcgc
    12001 ttcccgacgc tcgcgatcgc cgggctcgac atgaacgcgt tcctcgaacg taccgggctg
    12061 tccgaggagc actgctcgat cgtcgccgcc aagaaccgtt cgaacgcgct cgacaacccc
    12121 cgggccgcct atccggccga cgtcaccgcc gacgacgttg acgactcgag ccccgtcgcg
    12181 tggccgctga aggcgctcga ggtcgccgaa cgcgccgacg gctgcatcgt ggtggtgctc
    12241 gccgccgagg accgcgcgcg cgagctcacc gaggggccgg tgtggctgct cgggaccggc
    12301 tggtcctcgg ggagcccctc cctggagagc cggacgtggg gcgagaccga gccggtgacc
    12361 agggccgccg agtccgcgta ccgccgggcc gagatcgacg accctcagga ggagatcgac
    12421 ctggccgagg tggacgacac cttcgcgtac aaggagctcc tccacctgga ggcgctcggg
    12481 ctgggccagg ccccggagct cgcgcagctg gtcgaggagg gcgggctcga gccggggggc
    12541 gacctcccgg tgaacgtctc gggcggctcg cttgggcagg ggaacctgtt cgaggcgaac
    12601 ggcctcgttc ggctgctgga atgcgtcgag cagctccgcg gggaggcggg cgaacgccag
    12661 gtcgacgagg cgtacgtggc ggtcgcgcag tccgctcgca gcgcgccgtc cacctcggcc
    12721 gcggtcctgg tgctcgcgaa cgacgagggg gcaggccgat gaagctccgg gccctcgacc
    12781 acgtctggtt ctgggtgacc gacttggatc ggtcggtgcg cttctacgag gagcagctcg
    12841 ggctcgggct gcgggtccgg cacggcgacg aatgggccga gctcgaggcc ggcgcgatcc
    12901 acatcgggct ccacggcgcg cgcggggagg gcaacgtccc gcacggtggc acggccgtgt
    12961 tccgcgtcga cgacctcgac ctcgcgaaga cggcgctcga ggagcgcggc gtggacttcc
    13021 acgagcacct cggcgaggtg ccgggcatcg cgcgctacgc atcgttcgac gaccccgacg
    13081 gcaactcgat gcagctgatc gagtacatcc cgggagaggg ctagatggcc cggcgcgtcg
    13141 cggtcgtggg cgcggggatg acgagattcg tccggcgcgc gcaggagacc ggcaaggagc
    13201 tcgcgtggga ggcctcccgc gaggcgctcg cgtcgtgcga gctcccgctc gatcggatcg
    13261 acgccgtgtg cctgaccagc gcccccgacg cgttcgacgg cgtccacatg aagggggaat
    13321 acctgtcgga cggggcaggt gcgttcggcc ggcccttcat ccggacgtac gtgggcggtg
    13381 gcagcggcgt gttcacggcg atccaggcgt actacacggt cgcgagcggg atggccgacg
    13441 tcgtgctggc cgtggccgag gagaagatgt cttcgacgca gccgcacccg cagggcgcgt
    13501 tcctgacgat cttcgacaac atcctcgagc gcccgctcgg cccgaacctc ctgtggatct
    13561 tcgcgctcga gatgaaccgc tacatgaccg tgcacggcat cgacaagcgc gacatcgccg
    13621 ccgtcgcggt gaagaacaag cggaacgcgg ccgaccaccc ggccgcgctc ctcggccagg
    13681 ccggcatcac gatcgaggac gtgctcgcct ccgagacgct cgcgtggccg gtgcaacgcc
    13741 tggacgtctc gccgatcagc gacggcgccg tcgcgatcgt ggtggcgagc gaggaggccg
    13801 cgaagcagat gaccagcgac cccgtctgga tccagggcgt cgggtggaac ctcgacaccg
    13861 cctactggac caaccgcgac ctcgtgttcc ccgactacgt cgcgaacgcg gctcgcatgg
    13921 cctacgggat ggccggcgtg accgagccgc gcaagcagat ccacgtggcc gagccctacg
    13981 accccttcga ctacaaggag ctgcaccacc tcgagggcct actgctgttc gacaagggca
    14041 aggcgcccga ggcggcccga gacggcgtcc tcgaccgcaa gggcgatctg ccctcgtgcc
    14101 cgagcggcgg cctcctcggc gtcggcaacc cgatcgcggc ggccggcctg atgaagatcg
    14161 ccgagctctt ctggcagctc cgtggcgagg cgggcgcccg gcaggtcccg ggcacacccg
    14221 aacggggcgt ggcccaagcc tggggcgacc tcatgcaggt cgggaccgtc gtggtgatgg
    14281 gtcgcgaggg cccgcccgcg acggcgacgc agctcgagac gccgcccacc gcgacgccgg
    14341 cgacgtcgct ctcggacgcc gagttccgct cggccacggg cgccgtcgag gacgagatcg
    14401 gcgcacgtta cgcctgggat gcgggcgtcg cgatcggccg gttcctcgac gggctccgtg
    14461 acggacggat catgggtcgc gagtgccgga actgcgagcg cgtgctcgtg ccgccccgga
    14521 tgttctgcga gcgctgcttc cgcggcaccg acgcgtggaa gcaggtggag cacaccggcg
    14581 tggtgcagac gttctcgatc tgcaacgtgc ggtgggacat gcaacccctc gacgagcccg
    14641 agctccccgc ggtgatcgcg atcgacggct cggacggcgg gttcctgcac acgctcggcg
    14701 aggtcgaacg cgacgagatc aggatcggga tgaaggtgga agccgtctgg aagccggtga
    14761 gcgagcgcac cggctcgatc ctcgatatcg cctacttccg accacggcag ggggatccgg
    14821 ggagctcaac gagcgagcgc gaccgcgcga gcggtagccg cctaggaggg gacgcgagct
    14881 gatgctcgag aagatcgcgc ggcccgaaga gatccggcgg tgggaggggg acctgcccgt
    14941 tcgccaccgc tacacgccgg gtgtcgccgg cgacgcgttc ttcacggcgc tcaaggagcg
    15001 cggcgtgctg ctcggctcgc ggtgtgagag ctgcgcgtac acctatgtgc cggcgcggct
    15061 gttctgcgaa cgatgcttct cggagctctc ggccgacacc gaggtcggac ccgccggcga
    15121 gctcgtctcc ttcacgatcg tgttccaggg gatcgagggc gagccgctcg accgccccca
    15181 gaccctcggt gcggtccgtc tcgacggggc ggacgcggtc ctgatccatc gggtcgagcc
    15241 gggcgaggag ccgctggaga tcggcgaacg cgtcgaggtg gtcgtcgagc cggagagccg
    15301 ccgaaccgga tcgatcctcg atatcgcggg cttccgtctc gtcgagacct agaacatcgc
    15361 ccggctcgat ctaccccacg gctcccggag ccgggttcag ctcgccgcct tcttcacgag
    15421 cgcgccgatc ttcttctcat cggcggcggt cagcctcttc agcgcgaagg aggtcggcca
    15481 catggcgccc tcatcgaggt tcgctgcttc ctcgaaaccg aacgtcgcgt accgcgactt
    15541 gaatttgtcc gcactcttga agaagcagac gatctcgtcg tccttggcgt acgcgggcat
    15601 cccgtaccag gtcttgggcg aaaggctcgg cgcgctcttc gtgatgatcg cgtggagccg
    15661 ctccgccatg gcgcggtccg gttccggcat ctcggcgatc ttcgcgagca gggcgcgctc
    15721 cccctcctcc ttggtcttgg cggccttcag ctcccgggcg cgttccttca tcgcggctcg
    15781 ttcctcggcc gtgaacctgt tccccgcgcc ggtccgcttg gcggacgtcg tggtgccctt
    15841 cttcgcggtt gtcttctcag ccattgccat tgcacctcct tgcttcggaa acgtatgtgc
    15901 cgcttgacct ccggcgcgat cgccggattg ctcagcgcag ctcctggatg cggatcagat
    15961 tgcccgcggg atcgcggaag gcgcagtcgc gaaccccgta cggttggtcg gtcggctcct
    16021 ggacgacctc ggcgtcgccg gcctgcaggc gttcgaaggc gccgtcgacg tccttggtgg
    16081 ccaggttgat gctggcgaac gttcccttgg ccatcatctc cgcgatggtg cgccgctcgt
    16141 cgtcggtgat gccgggggtg gcggcgggcg ggtacaggac gatggacgtg ccgggctggt
    16201 cggggggccc gaccgtgatc cagtacatcc cgccgtattc gacctcgttg cggacctcga
    16261 agccgagggt gtcgcgatag aaggcccggg aggcgtccgg atcctcgtgc gggagaaggg
    16321 tctggtagat ggtgatatcc atggcggtca tgctacgtgc ggctcggcga gcggcgcttc
    16381 tcgattcctg atcggtctgg tcacctgttt cgccacgcac ggaggcatcc cggccatggc
    16441 gcgcggcgac tggcgccggt acgcgctggg cggcacaccc accagctcgg tgaagcgggt
    16501 gctgaaggtg cccagcgacg agcagccgac gtcgaaacag acctcggtga cgctcaggtc
    16561 gccgcgacgc agcaacgcca tcgcgcgctc gatgcgccgg gtcatcaggt agctgtaggg
    16621 ggactcgccg aacgcgagcc ggaactggcg gctgaggtgc ccggccgaca tgtgcgcccc
    16681 gcgggcgagc gcctcgacgt ccagcggctg agcgtactcg cggtcgatcc ggtcgcggac
    16741 gcggcgcaga accgcgaggt cgcgcaggtg ctgcgcggag gcggacctgc tggtcacgag
    16801 cgagatagta ctgtcgcgcc ggccgcttcc cagccgcacc cgcgcccggg tcgcgtcatc
    16861 cggcgcccgt atgctttcgg catggcgacc ctgacgtgcg agcggtgcgg gctccctctg
    16921 cccgacgagg cccggttctg cccgaactgc ggctatccgg tgggtgcccc ggcgccggag
    16981 gagcgcaagt tcgtcacggt gatcttcgtc gacctggtgg ggtcgaccca gctctcgagc
    17041 cagatcgatc ccgaacgcta ccgagaggtc ctcacccggt actaccggat ggtggcccag
    17101 gaactcgagt cgctccgcgg ccgcgcctac aacttcgccg gcgacgcggt cgtcggggtc
    17161 ttcggtctgc agcacacgca cgacgacgac gcgctccgcg ccgttcgggc cggcctgggg
    17221 ttggtcgacc gcgtcggtcg cctcggcgag gagatcgggc tcccggtccc gctccggatc
    17281 cgcgtcggga tccataccgg tccggtcgcg atcggcagcg aggcgtccga gcagggcctg
    17341 ctctacggcg ccaccgtcaa catggcggcg cggctccagc aggccgccga cccggggacg
    17401 gtgctcgtct cggaaacgag ctacctcctg tccctctcgc aggtccagta cggcccgatg
    17461 cgcgaggtcg aggcgaaggg gttcgaggat gaggcgcgcg cgtggccggt cgcggcgctg
    17521 cagccgggct cgggccgccg aacgatcccg ctcgtgaacc gcaagcgcga gctccggctg
    17581 ctgcaggacg cgttcgacgg cgcgcgggag acgcgccgcg ggcacctggt gacgttgttc
    17641 ggcgagccgg ggatcggcaa gagccgggtt gtcgacgagt tcgtggccgg tctgccggac
    17701 gacgccaagg tgctgatggg ccgcgcgagc cccttcgagg aggacgtgac cttcggaccg
    17761 ctcgcgcaga tcctcctgca cgagatgggg gaggcggcgc acgcgccgcc cgaggcgctc
    17821 cgcacgcggc tcgaggagat cgtcgtggag tccaccccgc ccgacgaggc gaagcagatc
    17881 gtcgagcgcc tgtgcatcgc gctcgggatc gccggcgagg cgcgcgacga gaagcgctac
    17941 cgcgtggccg agatccgttc ggggctgctc gcgctcctgc gcgggttctc cagccgggga
    18001 ccggtggtcg tggtgctcga ggacctgcag gcggcccagg cgccgatgct cgagctggtc
    18061 gaggagttgg tgcgcgaggc cgacggcatc tcgttgctcg tgatctgcgc cgcccggtac
    18121 acgctgctcg acgaccgtcc gggctggggc ggcggtcgcg ggaactcggt caacctctac
    18181 ctggagacgc tctcgctcga cgactcgacg cagctcgcgc gggacgcggg cgaaggcctg
    18241 gacgaggcga cggcccagcg gatcgcgcag cacgccggcg ggaacccgtt cttcatcatc
    18301 gagacgaccg ggatgctgct gcaccaaggg acggggctct cggccgacac cggcccgctg
    18361 ccgccggcgc tcctgccgcc gaccgtgcag gccgtgatcg cggcccggat cgaccacctc
    18421 tcaccgcagg gccgagatct gatccgcaag gcctcggtgt tcgcccgctc gtcgttcacc
    18481 ctcgccgagc tggacctgat cgccgttccg accggcgagg cgctcggccg cctcgaggag
    18541 gaggagctgc tcgaacacga cggcgatcgc ccggacgtct ggcgcttcag ccacggcatg
    18601 gtgcgcgacg tcgcctacga gagcctcccc aagcgcgagc gccagcgcct gcacctgttg
    18661 gtcgccgacg gcctgtccgg cgacccggag acggccgccc gccgccctcg cgcgatcgcg
    18721 ttccacctcg agcaggcggc cctggccgcg ctcgacctca acccgcgcga ccgaacgctc
    18781 gcggaacggg cggtcgacgc cctggctcgc gccggcgata tcgcccgtga ggggccgaac
    18841 atgaaggcgg ccgaggagga ctaccggcgc gcgctggagc tcgccggccc cgaacgcggg
    18901 tgggggctcc gcgaggcgag gatcctcgcg agcctgggcg agatccgcta ctggctcggc
    18961 gagttcgagc ccgccgtccc ggtgctcgag cgtgcgctga agctcggcga cggcgacccc
    19021 gcggtccgag cacaggcctc gcggttcctc ggcgacatcg agctctcgat cctgggtgac
    19081 gccaagcgcg ctgccgagct gctcgacgac gcgctcgagg cggcgcgcgg gctcggggat
    19141 ccgtggacgc tggcgcggac gctgctggtc gcggcctggg ctccctactt ccggcaggac
    19201 gccgagggcg cgcgctcgat gttccgggag gcgctcgaga ccgcgcgcgc gaacccggag
    19261 ggcgaccgct ggtccgaggc tcggtcgctc gtggggctct ccacgctcga gagcgacgag
    19321 ggcgacgagg agggctcgct ccggctcgcg gcggaggcgc tcgtcgtcgc cgagacgagc
    19381 ggagaccggt tctcgatcgc ggtcgcccac gaggcggttg gcggcacgat gcgtcggatg
    19441 atgcgtctcg aggacgccga gccgaacctg gacgccgccg tcgacgcgtt ccgcgagctc
    19501 ggtgcgcgtt gggagctcgc gagcgcgctc acgtcgcggg gcatcgctcg acggctcgcc
    19561 ggcagggcag atgacgccgt gaaggacctg cgcgaggcgt accgcctgtg tcgcgagctg
    19621 aaggaacgga gcatcatcac gtggacggcg agcgcgctcg cccgggcgtt cgtcaacgcc
    19681 ggggatccgg gctcggcacg gcgcgtgctg gacgaggtcg actcggtcgc caacgtccac
    19741 accgccgccg gtgaggactg gatcgactac gccgacgtcg agatcctgct ggccgagggc
    19801 gagcgggacg cagcgctcga acgcgcgatc tcgatcctgc ggtacgagcg cgacgagggt
    19861 tcggaaaagg acatcgcggc gcgcgtgtgg tggatctcgc aggtgttcgg ggtcgaggcc
    19921 gcgggtggcg agggggaggt cgagcgcgcc cgggagctgc tcgagcggac ccagatgctc
    19981 caggccttcc gcgaggccga gctggtcacg agccgctcgg cgatctcgcg ggcctgaccg
    20041 ctaggcttcg gacccgtgga cttcgacctc acgccggagc aggacgagct gcgggcggcg
    20101 gtccgcgcgt tcgcccagga gatcgtggcg cccgccgcgg ccgaggccga cgagcgcgag
    20161 gaattcccca ccgagatcgt gaagcgcgcc gccgagctcg ggctgttcgg gatcccgttc
    20221 cccgaggagg tcggcgggca ggggggcgac ctgctctcgt tctgcctgtg tctggaggag
    20281 atcgggcggt acgactcgtc gctcgcgatc acgctcgagg ccgcggtagg gctggcggcg
    20341 aacccgctgt tccggttcgg cacggatgag cagcgccagc gctggttggt atcgatggcg
    20401 aggggcgagg cgatcggcgc gttcgcgctc accgaacccg gcggcggctc ggacgcccgc
    20461 gcgatccgga cgaccgccag gttcgacggc gacggatggg tgatcgacgg ctcgaaggcg
    20521 ttcatcacga actcggggac gccgctgacg tcggtgatca tcgtggcggc gctggacgag
    20581 acggccgacg acggcgacgt ctcgacgatc gtcgtgcccg tcgggacgcc cgggctcacg
    20641 gtcggcgcga gctaccggaa ggtgggctgg cgcgcgagcg acacccacgc ggtctcgttc
    20701 gacgcgtgtc gcgtgccggc ggaccacctg ctcggcgagc gcggccgggg gtacgcgcag
    20761 ttcctcgaga cgctcgccga cgggcgggtc gcgatcgccg cgctctcggt gggcctcgcg
    20821 cgcggatgcc tggaggagag cgtccggtac gcggcggagc gggaggcgtt cgggcagccg
    20881 atcggcgcgt accaggcggt gcagttcaag atcgcggaca tgcgggtggg ggtcgagacg
    20941 gcgcggctcg cgtaccagcg ggcggcgtgg ctccgcgacc gagggcgccc cttcgcgacc
    21001 gaggcgtcga tcgccaagct gtacgcgagc gaggtcgcgg tctccagcgc ccgggaggcg
    21061 gtgcaggtcc acggcggcta cgggttcgtc gaggacttcc ccgtggcccg cttctaccgc
    21121 gacgccaagg tgctcgagat cggggagggc accagcgaga tccagcgcgt gctcatcgcc
    21181 cgggcgctcg gcctgccggg gaccgacctg taggcgccgc caggcacgga tcgcgtccga
    21241 gtctcgagcg gggcggggaa gaacgccggg atggttgcaa ccaagggttg cagcgggcac
    21301 ggttgacatc ccgctcggcc gtccgatagg aagggccttc cccatggccg aaaaccacag
    21361 cggctcaggc gtcgtgttcc gccgcgccct cgcggtggtg gtggcgctgg ccctcctgac
    21421 gtcgttcgcg caggtcgccc tcgccgagcc gagcacccag gagaagctcg acgctgccaa
    21481 ggccgagttc gagcagctga agaaccagat cgcgagccag cagcaggtcc tgaaccggct
    21541 gtcggcggag gcagccgtca tcgccgagcg ctgggacgtc gcgagcggcc gctgggagca
    21601 ggtcacctcc gagctccgca agacgcagct cgccctcgcc gaggcccaag cggagtacca
    21661 gggcctcaag tccgacctgg aggaacgcgc ccgccaggcc tacatcaccg gcccggggtc
    21721 gggtctcgag ttcctgctgg gcgcgtcgtc catcgcggac ctgtcggcgc gcatggagta
    21781 cgtgaacgcc ttgagccagg aggacgccga cctagcgacg gaggtgcaga acgtccggaa
    21841 cacgctcgcg gcgcagaagg aagatcagga gaagctgcag gcgaaggcgg cgaaggctct
    21901 gaagaaggtc gagagcgaac aggcagcgct cgacgcgaag ttcgacgagc agcaggcggt
    21961 gctcgacgac ctggacgcga agaaggcgcg ggccgaggac ctggtgaagc gactcaccaa
    22021 gcgtcagcag
//
LOCUS       DATBRK010000101         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_733488, whole genome shotgun sequence.
ACCESSION   DATBRK010000101 DATBRK010000000
VERSION     DATBRK010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733488"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /gene="purF"
                     /locus_tag="VIH70_03085"
     CDS             <1..1296
                     /gene="purF"
                     /locus_tag="VIH70_03085"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY8115377.1"
                     /translation="QHRGQEAAGMAVSDGEHILVFKELGLVSQVFDEATLSTLRGDLG
                     IGHNRYSTTGSTTWENAQPAFKTDGQRSLALGHNGNLVNTLELAERVGAGGSATTDSD
                     LVATILARHPSEDLESALIDVLPTLEGAFSFVVMDERSLFAVRDPHGLRPLSIGRLPN
                     GFCVASETCALDIVGATFLRDVEPGELVRVDERGVVSTRFGNSERGALCIFEFVYLAR
                     PDSRLRGTTVHEVRRELGRRLALEAPAEGDLVIPVPDTAHSAASGYAEVSGIPYGEGL
                     MKNRYVGRTFIQPSQSLRERGVKLKLNPMPDAVRGKRLVVIDDSIVRGTTTRQIVQAL
                     REAGATEVHTRITCPPIRWPCFYGIDMSTRAELIAAGSTVDGVRDFVGADSLGYLSLE
                     SLVKATGAPTNSFCRACFDGHYPVPVGEEEPSKLALETL"
     gene            1293..>1523
                     /locus_tag="VIH70_03090"
     CDS             1293..>1523
                     /locus_tag="VIH70_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115378.1"
                     /translation="MTEGVQGGSSNERSEAAADPLAREASHRWRNASEETEGKAAYRQ
                     SGVDVQAGRRAVELIRDMAHAARRPEVVGAVGD"
BASE COUNT          225 a          522 c          549 g          227 t
ORIGIN      
        1 cagcaccgag gccaggaggc ggccgggatg gcggtctccg acggcgagca catcctggtg
       61 ttcaaggagc tggggctcgt ctcccaggtg ttcgacgagg cgacgctctc gacgctccgc
      121 ggcgacctcg ggatcgggca caaccgatat tcgacgaccg gctcgacgac gtgggagaat
      181 gcccagcccg cgttcaagac ggacggacag cgttcgctcg cgctggggca caacggcaac
      241 ctcgtgaaca cgctcgagct tgccgagcgg gtcggcgccg gaggctcggc caccaccgac
      301 tcggacctgg tcgcgacgat ccttgcccgg cacccgtcgg aggacctcga gtcggcgctg
      361 atcgacgtgc tccccacgct cgagggcgcg ttctcgttcg tcgtgatgga cgagcggagc
      421 ctgttcgcgg tgcgcgatcc ccacggtttg cgtccgctct cgatcggccg cctgccgaac
      481 ggtttctgcg tcgcctccga gacctgcgcg ctcgacatcg tgggcgcaac gttcctgcgc
      541 gatgtcgagc cgggtgagct ggtgcgcgtg gacgagcgcg gcgtcgtctc cacgcggttc
      601 gggaacagcg aacgcggcgc gctctgcatc ttcgagttcg tgtacctcgc gcggccggac
      661 tcgaggctcc gcggcacgac ggtgcacgag gttcgtcgcg agctcgggcg gcgcctcgcg
      721 ctcgaggcgc cggccgaggg cgacctcgtg atcccggtcc ccgacaccgc gcattccgct
      781 gcgtcggggt acgcggaggt ctccgggatc ccctacggcg agggcctgat gaagaaccgg
      841 tatgtcggcc gcacgttcat ccagccgtcg cagtcgctgc gcgaacgcgg cgtgaagctg
      901 aagctcaacc cgatgcccga cgcggtgcgc ggcaaacgcc tcgtggtgat cgacgactcg
      961 atcgtccgcg gcaccacgac acgccagatc gtccaggcgc tccgcgaagc aggcgcgacc
     1021 gaggtgcata cgcgcatcac ctgcccgccg atccggtggc cctgcttcta cggcatcgac
     1081 atgtcgacac gggcggagct gatcgccgcc gggtcgacgg tcgacggcgt acgcgacttc
     1141 gtcggcgcgg actccctggg ctacctcagc ctggagtccc tggtgaaggc aaccggggcg
     1201 ccgacgaact cgttctgccg ggcctgcttc gacggccact acccggtccc ggtcggggag
     1261 gaggagccgt cgaagttggc tctcgagacc ctgtgactga gggggtccag gggggtagca
     1321 gcaacgagcg cagcgaggcg gctgcggacc ccctggctcg cgaggcgtcc caccggtggc
     1381 gaaacgcctc cgaggagact gaggggaagg cggcttaccg gcagtccggc gtcgacgtcc
     1441 aagcgggccg ccgtgccgtc gagctgatcc gggacatggc ccacgcggct cggcgaccag
     1501 aggttgtcgg cgcggtcggc gac
//
LOCUS       DATBRK010000102        18108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_158709, whole genome shotgun sequence.
ACCESSION   DATBRK010000102 DATBRK010000000
VERSION     DATBRK010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158709"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..499)
                     /locus_tag="VIH70_03095"
     CDS             complement(317..499)
                     /locus_tag="VIH70_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115379.1"
                     /translation="MSMSLATLAREEGVRWFSIGSDAHNELELELLPYGMATAARAGI
                     ETRSHPDHGSIDFLPS"
     gene            1078..1950
                     /locus_tag="VIH70_03100"
     CDS             1078..1950
                     /locus_tag="VIH70_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8115380.1"
                     /translation="MDHVSFSVHRGEVFGFLGPNGAGKTTTLEIVEGLTTPTSGRTRV
                     LGMDSVIQRDEMQQRIGVQLQSSAYFEYLTLEEILDLFGSFYERSLKPEPLLEQVGLL
                     EKRTSLVKHLSGGQAQRFSIIAAMVNDPEVVFLDEPTTGLDPQARRNLWDLIAKINGE
                     EAKTIVMTTHYMEEAQVLCDRVAIIDHGRIQALDSPLGLIHELPSAYRIQFATGADVD
                     TNELRALPGVHAVSPMLLGGFNYELAIVQTRETLPAFLDWTKSSGVEIDDVQVVSATL
                     EDVFLSLTGRSLRE"
     gene            1956..3002
                     /locus_tag="VIH70_03105"
     CDS             1956..3002
                     /locus_tag="VIH70_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8115381.1"
                     /translation="MIRLFNASVRMIYRDRQVLFWALVFPIIFAVVFGLFSFEDAPEV
                     EIELHGDRASPLYQAIDEGLVSIEPFTVTESADPAADNVVNGEMDVAVTVDGTSVHVR
                     CNQTNFDINRFAIAAIDQVVDEANLEAAGVNEPAVTVETTPVAGEDVTYYDFLLPGLV
                     AMGLMNASIGGMAVAISRFREQKILRRILATPLDPNRFLAAQVGARLVLALVQTALIL
                     GVGVFIFGATIHGNVVWLFVLAVIGNLIFLNIGFSIAGRARNPDAADGMANAIALPML
                     FLSGTFFPTDTLPEAVQTIVAILPLTPLLEAMRIVAVDGDPITAASSQILLLAGWVVV
                     SFAIASRLFRFQEV"
     gene            complement(3789..5153)
                     /locus_tag="VIH70_03110"
     CDS             complement(3789..5153)
                     /locus_tag="VIH70_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8115382.1"
                     /translation="MGSERATARTTGEVRRRLAAGGIALALIAGACDAEPATGRRPSP
                     TGAPGSTMTVPPTTSPVPPARADGSGPTRWPIEHVVFLIKENRTFDNLFGTFPGANGV
                     TVGYDRGVRRSLTPGTDGSIGSEDIPHCYTCSLEAWNHGKMDGFNQGAVSDRWAYTQL
                     HRGQLPTYWHWAREFVLADNFFSSAQGPSFPNHLYSIAAQSGGAHDNPRRTGPFRSSN
                     TFGCDAPKGQTVTIVDSEGHEKKVQPCFDFLTEGDLLTKAGIDWAYYAAEPDQLGYIW
                     SAYSAIRHIRETREWSRHVLPVDDVIGDIRDDLLPPVTWITPRYEVSEHPQYNFCLGE
                     NWSTRVIDAIMRSPMWKNTAIFVTWDDYGGFYDHVPPPQVDGFGLGIRVPLLVISPYA
                     KGGVIDHQIGEFSSVLRFIETNWGLSLLTDRDREATDLSYDFDFTKRPNPPDPLPLRT
                     DCFA"
     gene            5297..6793
                     /locus_tag="VIH70_03115"
     CDS             5297..6793
                     /locus_tag="VIH70_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8115383.1"
                     /translation="MDWKDEDPGLPIKFGPCSNAEYDPEPLPPVLRETIRRAREATER
                     NAHRVGMSRREFLLSICGAATTLLVLDACTREAVRTVSPGATPGGGYAIPPEATTDPE
                     VAHEVIGGEEFVFDIQGHLLEYDLNPILNGQEFWQRFPQQNCGEDDPRVCYSIESFLE
                     LMFLRSDTSMLVLSALPIYPEGSPLSTEIMDETRRIAEGLCRDERVLLHAQALPNLDP
                     SESSLDAMDETVDRYPIAAWKTFTHFPDVFENNGKAWWLDDHDPSLPQVGDRFIERAL
                     RLGVPTICAHKGFSGESPYASPQDIGPAARRHPEANFVVYHSGFEAGLAEGPYTAATA
                     NVGVNRLVTSMRKAGIGPNENVYAELGSTWWYIMRYPVQAAHVLGKLLKYVGEDNVLW
                     GTDCLFYGSPQDQIQALRSFKISEEFQERYGYPQLTRQIKNKILGANGARLYGVEPIT
                     DRCEFTRRELERIRRHLPGRNRTLGPSTTGEARAFRALDHAAIVQDPG"
     gene            7037..7789
                     /locus_tag="VIH70_03120"
     CDS             7037..7789
                     /locus_tag="VIH70_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115384.1"
                     /translation="MPKDAERFVERAFQVLFERAADAGDRARREAARGPSPDQAFEDG
                     RVLAFYEVIRELWTLAKELGLHERGLGAPRWHPDDVGVLRRDDPETQDRILTSLQFMA
                     RSDWEAQERFLGEHANVREALGERFAGTWIRWERHQREWHVAVVDPTPDDVELVADAA
                     RRAGFVPFVRGVRYSEAEVESFRRAGEAALDRMRSSPAHDVLRGYGPDAEANGVRLTI
                     SEANTTVVADLLAVMPVDVVEIEVNDIRAVAL"
     gene            7932..8249
                     /locus_tag="VIH70_03125"
     CDS             7932..8249
                     /locus_tag="VIH70_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115385.1"
                     /translation="MAMSRETKPGKIGAWIALGLSIALAVRVIAIDNPEALVIVAPAL
                     VGALLAVAWPQMRVALLTGLVLIGVTATFSLIGWIGLLFIPSMVLIGLSAYRISRTPT
                     LQP"
     gene            complement(8875..9258)
                     /locus_tag="VIH70_03130"
     CDS             complement(8875..9258)
                     /locus_tag="VIH70_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115386.1"
                     /translation="MKHAVDAGDGSVGERPVVPAAGSQEVGVEVVDIGSHELGQVEVA
                     DVRLEVAVDDGAGVAHCRGRPAGRGGLEPLVEQTRECAGSYPSPTGLGHQLLKLSAGE
                     AIGAVDRLGQPSLPPSIGVDALVDA"
     gene            9296..10051
                     /locus_tag="VIH70_03135"
     CDS             9296..10051
                     /locus_tag="VIH70_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY8115387.1"
                     /translation="MNPASNATPPRVAPAEIRPPSPEQVAVLLEWAKRRDPALYCFLR
                     LAVWTGARRSQLLALRWGDVDQERAAIAFSRALVEGPDGPELRSTKTHRTYRVELDAE
                     SLDVLTAHRHWARTKARDAGAELTAGAFVFSSRPEGAKPWLPNWVTKRFIDARRAAGL
                     PHFRLHDLRHFMASEMLAAGVPIVTVSQRLSHARASTTLNVYAHSVPGGDRRAAETLA
                     VILAAGRGHADRSDGRTTRRFRGTAERDGGTVG"
     gene            10229..10987
                     /locus_tag="VIH70_03140"
     CDS             10229..10987
                     /locus_tag="VIH70_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY8115388.1"
                     /translation="MYAVERRQEIATLIEERGRGDVTELADRFDVTPETIRRDLTELE
                     QRRLLRRVHGGAISIHRFRSEPAIAEKAEAMADEKAAIAAAATAFVPEAGSVLIDAGT
                     STLALARIFPERELRVFTHSVAVALELASKLDLRLYVIGGRLRTRTLANVDEWALRQL
                     AALRVDVAFVGTNGFSAERGLSTPDPAEAAVKHALCLAGAQTVVLADHSKVNQESAVR
                     FAAIDEFDVLISDHGLAEPYRRALDQAGVEVVLA"
     gene            11088..12230
                     /locus_tag="VIH70_03145"
     CDS             11088..12230
                     /locus_tag="VIH70_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014693320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HEY8115389.1"
                     /translation="MATAMPVPAGATGGWRARVQRFGGFLAGMVIPNIGAFLAWGLIT
                     AFFIPTGWIPNEDLAQLVDPMIFYLLPILIGFTGGFLIHGHRGGVVGAIATAGVAIGG
                     SVGTEVGGELVRTPMFLGAMIMGPLGGWLIKQFDQAVMHRVRTGFEMLVSTFSAGILG
                     AILAVLAYLLIGPPVSVLSNAAGNAVEFLVDNTLLPLTSIIVEPAKVLFLNNAINHGV
                     LAPLGVAEAAEEGKSILFMIETNPGPGLGLLLAYWMFGPRLLRPSVPGAIIIQFAGGI
                     HEIYFPYVLMRPITIVGVIAGGMAGVFTFLATDSGLTATPSPGSIFAYLAVTPKGGLP
                     GVLLGITVATAVSFAVNALILKATVREQDEVVIPEIVPTGPAPATA"
     gene            12230..12538
                     /locus_tag="VIH70_03150"
     CDS             12230..12538
                     /locus_tag="VIH70_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HEY8115390.1"
                     /translation="MASVQGSDVRKVVVACEAGMGSSVLLVTQLRDRLKGTGAEVVHS
                     PVSQVPADADVIVCHRGLEATARKIAPEKVVVTFNMFLGDPAFDRVVEAITEGKALEG
                     "
     gene            12541..12984
                     /locus_tag="VIH70_03155"
     CDS             12541..12984
                     /locus_tag="VIH70_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY8115391.1"
                     /translation="MDALLPAAGIRLGLQASDRFDAVRQSGQVLIDIGAVKAPYVDAM
                     LERERGISTSLGEGFAIPHGTEESRRWINETMLSFLQFPAGVEWDDVMVTVCIGIAAV
                     GDEHVGVLANLARVLTVPEQAASLRAAERPEDVLAILGRDEGAIG"
     gene            12981..14033
                     /locus_tag="VIH70_03160"
     CDS             12981..14033
                     /locus_tag="VIH70_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018745611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HEY8115392.1"
                     /translation="MKVARLYAPGDLRIEDADVPEAGPGDLVIRGRTCSTCGTDAKIF
                     RFGHHHISLPRVLGHEVAGEVTEVGSEVGVQGGHRWSPGDRVQIIAAIPDGVCFLCRR
                     GQHTVCEELESIGYQYDGGFAESMRVPAKVLAVDGVNRIPEHVPFEEASLTEPLACVL
                     NGQELAQVGEGDAVVVLGAGPIGCLHVRLARARGARMVVLIDVNQGRLDLAARAEPDA
                     AIDSTKDDPIDAIKKLTDGRGADVVITATGVGQAQEQALEMAALRGRISLFGGLPRDD
                     SVIRFDSNLVHYRELSVVGAYGSAPRHNREALALIADGKVRVDDLITHRMPLTDVSRA
                     IETVMSGEGLKVVIEP"
     gene            14038..15006
                     /gene="pfkB"
                     /locus_tag="VIH70_03165"
     CDS             14038..15006
                     /gene="pfkB"
                     /locus_tag="VIH70_03165"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEY8115393.1"
                     /translation="MIVTVTPNPSLDRTLEVEELRRGEVVRAHADRSDPGGKGVNVSR
                     ALAANGYPSRAVLPLGGHDGALLASLLEGLGIEVAAVPIADAIRSNVTVAEPDGTVTK
                     LNAPGPRLSPAEVDALLERAVAAADGAGWVVASGSLAPGTPDDLYARLAAAVRPVGVR
                     VAVDTSGPALERALVAGPDVVKPNEEELTEVTGVVPETLGDVVLAANQLREKGVGTVL
                     VSLGARGAVLVEDGEAVHAESEVIVPRSTVGAGDALLAGFLAAGGEGPDALAEGVAWG
                     AAACVLPGTAVPSPGDLQRQLVQTHPVDPERSLALGTDRRRRSTHA"
     gene            14999..15286
                     /locus_tag="VIH70_03170"
     CDS             14999..15286
                     /locus_tag="VIH70_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY8115394.1"
                     /translation="MPEARVTLRNPTGLHARPAKIFAKAAAAFPADVVVEKDGRRVNA
                     KSTLSLLTLDCHQGDEILISVEGEAADETLAELVGLVEAGIGEHEGGGVAT"
     gene            15283..17019
                     /gene="ptsP"
                     /locus_tag="VIH70_03175"
     CDS             15283..17019
                     /gene="ptsP"
                     /locus_tag="VIH70_03175"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY8115395.1"
                     /translation="MSTRLSGRAAAPGAAVAPAFVLAPQPVLTKLPEVASGAPEDERT
                     RLLGALERAEAELRELAERVAASAGEEEGEIFEAHAEFAADPELARLTEEAVRAGASA
                     ERAVVGAFETFRELLVASASEYLAARASDLDDVRDRVVKILLGMSTGGDRPEQRSVVV
                     AHELTPSETASIPVDLIAAIVTETGSPTSHAAILARALGVPAVVACDGVLAATRAGVH
                     IAVDGRAGEAIVDPTPDERAEIVRRHEQEERRREDLRALREKPGRTADGHPVELAANI
                     GSIDHIPAAVEAGGQGSGLVRTEFLFIGRVTAPTVEEQTKVYAEILRGFPGHRVVFRT
                     MDVGADKPLPFVERDPEENPALGLRGIRLSLRSPDLFRDQLRALVRARADVAGEEAGR
                     LAIMFPLVATAGELEAARGVLRGVADEDGVDLEGIEVGVMIEVPSGALGAARLARHAD
                     FFSIGTNDLIQYLFAVDRLNGAVADIGDVLEPDVLTLIGSVVEAGHANEAWVGVCGEA
                     AGDPTVAGALVGLGVDELSMTKVAIPEVKDALRRLTRDECREAVHAAIAGAADAAGSR
                     TILEERLRTSLA"
     gene            17179..17778
                     /locus_tag="VIH70_03180"
     CDS             17179..17778
                     /locus_tag="VIH70_03180"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY8115396.1"
                     /translation="MHMYDQQLVRVHATSIHADVWHMMRGLPYVMRFMGAGLRGPKDV
                     VPGTDLSGVVERVGKGARRFQPGDEVFGQTLVANLWRHGAVRRTRRRSGVEVRAEAGG
                     PHVRTGRSRPTSGSLAVQGVRDEGRIQAGHRVLINGAGGAVGTFAVQLAKALGAEVTA
                     VDAAEKLEMLRAIGADRVVDYGQKDFTGATRDGHVPETV"
BASE COUNT         2871 a         5925 c         6356 g         2956 t
ORIGIN      
        1 ttcggggact acacgaagct ggcgttcgat tcgggaacgt tctatccagc gtggtccgac
       61 aactcgaaca gcaccgggga caaccccgac ggcgcgctgt ccaaactgga tctctacacc
      121 gcggcggtca cgatctcgta ggcaccgaga ccggccgcag aggcgtccgg taccggtcaa
      181 gctggccggg caagctacgc gctttgcctt cacctgtacg gtgaaggagc gacgacaagg
      241 ccatggacaa gatcacgcac ggatggtcgc atcatgtctg gtgctcgatt cgtgcttctt
      301 cgttgagatc gcgcgcctac gagggcagga agtcgatcga tccgtggtca ggatgcgatc
      361 tcgtttcgat gccggcgcga gcggcggttg ccatgccgta cgggaggagc tccagctcga
      421 gctcgttgtg cgcatcgctt ccgatcgaga accaccggac gccctcctcg cgggcgagcg
      481 tcgccagcga catgctcacg tcctggcgcc actccgtggc gtccagctcg acggctttgc
      541 ccagccgtgt gcagcctcgc ggaagttcat gtatcgcgag ctatccggcc agcttcggaa
      601 ggcgcgcttg gctggccgcc caccacgacg ggtggcctag attggtcctc cctaaccatc
      661 ttgaccaggt gcgccctgtc gcgccctgag caggcgcaac cgcgtcgcac ggttgtgtcg
      721 gagctgtcca tcagggcggg tccgagacct ctcgtggtgg cccaagtctg cccacaggct
      781 gatgggctca cggccctgtt gggaccatca agaacgccca ggtcagggcc catgccggtc
      841 cgggtgcggc agttgtcgca gcgaacgtct ggacacacgc aacctctcta tcctcttgac
      901 ctgcggagat gtaggcagaa gcctctgtag agcggccaca ggctgggagc tgtcacccgg
      961 gagttgaccg atgggcgagg agcgcgtggg cgaggggcgc gtgcgtgacg aggtcgcggt
     1021 cgaggtccgc gatctcgtga aggagtaccc gacccccgag gggccagggt cgccgcggtg
     1081 gatcatgtct cgttcagcgt ccatcgcggc gaggtgttcg gcttcctcgg tcccaacgga
     1141 gccggcaaga cgacgacgct cgagatcgtc gaggggctca ccacgccgac gtccggccgg
     1201 acccgggtcc ttgggatgga ctcggtgatc cagcgtgacg agatgcagca gcggatcggc
     1261 gtccagctgc agtcgagcgc gtacttcgag tacctcacgc tcgaggagat cctcgacctg
     1321 ttcgggtcgt tctacgagcg ctcgttgaag cccgaaccgc tgctcgaaca ggtcggcctg
     1381 ctcgagaagc gcacgtcgct cgtgaagcac ctctctggtg gccaggctca gcggttctcg
     1441 atcatcgcag cgatggtgaa cgatcccgag gtggtcttcc tcgacgagcc gacgactggc
     1501 ctggacccac aggccaggcg gaacctctgg gacctgatcg cgaagatcaa cggcgaagag
     1561 gccaagacga tcgtgatgac gacccattac atggaggagg cgcaggtcct gtgcgaccgc
     1621 gtggccatca tcgaccacgg gaggatccag gcgctggatt ccccgctcgg cctgatccac
     1681 gagctcccat ccgcgtatcg gatccagttc gcgacgggcg ccgacgtgga cacgaacgag
     1741 ctgagagcgc tgccgggtgt tcacgccgtc tcgccgatgc tgctcggtgg cttcaactac
     1801 gagctcgcaa tcgtgcagac tcgggagacg ctgcccgcgt tcctggactg gacgaagtca
     1861 agcggggtgg agatcgacga cgtgcaggtc gtgtcggcga cgctcgagga cgtcttcctc
     1921 tccctgacgg gacgcagcct gagggagtaa agaccatgat caggctcttc aacgccagcg
     1981 tgaggatgat ctaccgcgac cgtcaggtgc tgttctgggc cctcgtcttc ccgatcatct
     2041 tcgcggtcgt cttcggcctg ttcagcttcg aggacgctcc cgaggtcgag atcgaactgc
     2101 atggggatcg ggcatcgccc ctgtaccagg cgatcgacga ggggctggtg agcatcgagc
     2161 cgttcacggt gaccgagagc gccgacccgg ccgcggacaa cgtggtgaac ggtgagatgg
     2221 atgtggccgt gacggtcgat ggcacgtcgg tccacgtgcg ttgcaaccag acgaacttcg
     2281 acataaaccg cttcgcgatc gccgcgatcg accaggtcgt ggacgaggcg aacctagagg
     2341 ccgccggggt taacgagccc gcggtgaccg tggaaacgac cccggttgcg ggcgaggacg
     2401 tcacgtacta cgacttcctt ctgcccggcc tcgttgcgat gggactgatg aacgcctcga
     2461 ttggcgggat ggcggtggcg atctcccggt tccgggagca gaagatcctg cgccgcatcc
     2521 tcgcgacgcc gctcgacccg aatcggttcc ttgcagctca ggtcggtgcg cggctcgtcc
     2581 ttgccctggt gcagacggcg ttgatattgg gggtcggagt attcatcttc ggggccacca
     2641 tccacggcaa cgtcgtgtgg ctcttcgtgc tggcggtgat cgggaacctg atcttcctga
     2701 acattgggtt ctcgatcgcg ggacgtgcgc ggaaccccga tgccgccgat gggatggcga
     2761 acgcgatcgc gctgcccatg ctgttcctgt cgggtacctt tttcccgacg gacacgctgc
     2821 cggaggcggt gcagacgatc gtcgcgatcc tgcccctcac accgctcctg gaggcgatgc
     2881 ggatcgttgc cgtcgacggc gatccgatca ccgccgcgag cagccagatc ctgctgctgg
     2941 cggggtgggt ggtggtgtcg ttcgccattg cgagccgcct gttccggttc caggaggtct
     3001 agcgaagggc aatcgggaat gaacctctaa ccgccggcac tccgctctag tcgtgcagcc
     3061 accaagcgcg tggttcacgg ctcacccctg cgtgagtttt ggtcgacatg gcggaagatc
     3121 gccacggcat gcattggatt cgcttgacca tgcagtgaag ccaagagcgt tcacggtaga
     3181 tgcgttgaag cggtgagtct ggggtccgtc cttgccatcg gcttcaatcc cgctggcgcc
     3241 catgttgcga gtctaggtcg atacgacgcc cgtccggcgc gggcttccgg ttcggccaaa
     3301 aacagggagc cccgcaacgc gaacgctaag ggctcccgac cttctctcgt tgctcgctct
     3361 cctagtccgc gaccgggatc acgaacaccg tcaacgcaca gttccccagc cccgcactcg
     3421 ggttgttgtt ggtgtttccg cctccgaccg ggccaggcac cggatcggtg cgtgcgtcca
     3481 cgaagacatc gtaggcgcca ggcgcgacgt cgtccgtacg cgacccggcg cgttcctcgg
     3541 ctcttgacgg acaacggtat aggggtctcc tttggcgatg cggtcggagg acggtgggat
     3601 gatgaagcgg cttctgagtg ccttcgcgga cggactccgg gcgaaggcgg cgacccgacc
     3661 gaaacgggat ctcaatcggg ctgcagagaa gaagaagacc catgaggaga tgacttcccg
     3721 tgattgacag ggggaacctc ttgacctcca ttgcggaacc tgcggtttcg cggtgttccc
     3781 cacagacctc acgcgaagca gtccgtgcgg agcggcagtg gatcgggcgg gttcgggcgc
     3841 ttcgtgaaat cgaaatcgta cgagagatcg gtcgcctccc ggtcgcggtc cgtcaacagg
     3901 gagagccccc agttggtctc gatgaagcgc agaacgctcg agaattcgcc gatctgatga
     3961 tcgatcacgc cgcccttcgc gtagggagag atgaccagca acggcacgcg tatgcccagc
     4021 ccgaatccgt ctacttgcgg tggtggcacg tgatcgtaga agccgccgta gtcgtcccat
     4081 gtcacgaaga tcgccgtgtt cttccacatg ggacttcgca tgatcgcgtc gatcacccgt
     4141 gtcgaccagt tctcacccag gcagaagttg tactgggggt gctcggagac ctcgtaccgc
     4201 ggggtgatcc aggtgaccgg cggtagcagg tcgtcccgga tgtcgccgat gacgtcgtcg
     4261 accggcagca cgtgccggga ccactccctc gtctccctga tgtgccggat ggccgagtat
     4321 gcggaccaga tgtagccgag ctggtcgggc tcggccgcgt aataggccca gtcgatgccg
     4381 gccttcgtga ggagatctcc ttcggtcagg aagtcgaagc acggctgtac cttcttctcg
     4441 tgaccctccg agtcgacgat cgtgaccgtc tggcccttcg gtgcgtcaca gccgaacgtg
     4501 ttgctcgatc ggaacggccc ggtgcgcctg gggttgtcat gagcgccgcc ggactgggcg
     4561 gcgatcgagt acaggtggtt cgggaacgag ggcccctgcg ccgaggagaa gaagttgtcc
     4621 gcgagcacga actcgcgagc ccagtgccag taggtcggga gctgccctcg gtggagctgc
     4681 gtgtaggccc atcggtccga cacggcgccc tggttgaacc cgtccatctt cccgtggttc
     4741 caggcctcga gtgagcatgt gtagcagtgg gggatgtcct cggagccgat gctgccgtcg
     4801 gtcccgggtg tgagcgaccg ccgcacgccg cggtcgtagc cgaccgtcac gccgttggcg
     4861 cccgggaacg tcccgaacag gttgtcgaag gtgcggttct ccttgatcag gaagacgacg
     4921 tgttcgatcg gccatcgcgt ggggccgctc ccgtccgccc gggcgggcgg cacgggggac
     4981 gtggtgggcg gaacggtcat ggtcgatccg ggagcccccg taggcgacgg gcgccgcccg
     5041 gtcgcgggct ccgcgtcaca ggccccggcg atcagcgcga gcgcgatccc tccggcggcc
     5101 agacgccggc gcacctcccc ggtcgtgcgt gccgtggccc gctcggatcc catcgcgatg
     5161 cagactcgat caggcccccg gacgttcccg tcggggaagg gtccggtcgg ctccacgggg
     5221 ccggagggaa ggccgccgct gcgcggaacc gggacggcct tggcgctgtc tatcgtgggt
     5281 cttgatcggg ggcgtgatgg actggaagga cgaggatccg ggactaccga tcaagttcgg
     5341 gccgtgctcc aacgccgagt acgatcccga gcccctcccg ccggtgctga gggagacgat
     5401 cagacgggcg cgcgaggcca ccgagcggaa cgcgcaccgt gtcgggatga gccggcgtga
     5461 gttcctgctg tcgatctgcg gcgccgccac gacgcttctc gtgctggacg cgtgcacccg
     5521 cgaggccgtg cggacggtct cgccgggcgc gacgcccgga ggcggctacg ccatcccccc
     5581 cgaggccacg accgaccccg aggtcgccca cgaggtcatc ggtggcgagg agttcgtctt
     5641 cgatatccag ggccatctcc tcgagtacga cctgaacccg atcctcaacg ggcaggagtt
     5701 ctggcagcga tttccgcagc agaactgcgg cgaggacgac cctcgagtct gctactcgat
     5761 cgagagcttc ctggaactga tgttcctgcg aagcgacacc tcgatgctcg tgctgtcggc
     5821 gctgccgatc tacccggagg ggagcccgct ctcgaccgag atcatggatg agacccggag
     5881 gatcgccgag ggcctgtgcc gggacgagcg ggtgttgctg cacgcgcagg cgctgccgaa
     5941 cctcgatcca tccgagtcct cgctcgacgc gatggacgaa accgtcgacc gttatcccat
     6001 cgcggcgtgg aagaccttca cccacttccc cgacgtcttc gagaacaacg gcaaggcttg
     6061 gtggctcgac gaccacgacc cgtcgcttcc gcaggtcggt gatcggttca tcgaacgcgc
     6121 cctgcgtctg ggggttccga cgatctgtgc gcacaagggg ttcagtggcg aaagcccgta
     6181 cgcctcgccg caagacatcg ggccggcggc gaggagacat cccgaggcga acttcgtcgt
     6241 gtaccactcg gggttcgaag caggtctcgc ggagggaccg tacacggccg cgacggccaa
     6301 cgtcggggtg aaccgcctgg tcacgtccat gaggaaggcg gggatcgggc cgaacgagaa
     6361 cgtctacgcc gagctcggct cgacctggtg gtacatcatg cgttacccgg tgcaggcggc
     6421 gcacgtcctc ggcaagctct tgaagtacgt cggcgaggac aatgtgttgt ggggtactga
     6481 ctgcctcttc tacggctcac cacaggatca gatccaggcg ctccggtcct tcaagatcag
     6541 cgaggagttc caggagcggt acggctatcc acagctcacg cggcagatca agaacaagat
     6601 cctcggtgcg aatggagcgc gcctgtacgg cgtggagccc atcacggacc gatgcgagtt
     6661 cacccggcgc gaactcgagc ggatcaggcg tcacctgccg ggccggaatc gcacgctcgg
     6721 cccgtcgacg acgggggagg cgcgagcctt ccgagccctc gaccacgcgg ccatcgttca
     6781 ggaccccggg tgattccgaa gtagcgacag gtccgcggaa ggatgccggt cacctcctta
     6841 gcgcggcgga gatcgccttg ggaggcgacg cgctggacga tccgatcgcc tggccttgcg
     6901 ggcttcacgc agcgatgcac cgaagagaca gccgggaatg ggagaccgca tttgcatgga
     6961 tctggcgcaa tagagtcaat gtgacctgat ctggttcgcg atcccctcga tcgccatgac
     7021 gcattggagg caccgcatgc cgaaggacgc ggagcgattc gtcgaacgag cgttccaggt
     7081 gctgttcgag cgggcggccg acgccggaga ccgggcccgg cgggaagccg cccgcggccc
     7141 gtcgcccgac caggcattcg aggacgggcg ggtgctcgcc ttttacgagg tcatccggga
     7201 gctctggacg ctcgcgaagg agctcggcct gcacgagcga gggctcggcg cgcccagatg
     7261 gcatccggac gacgtgggcg tccttcggcg agacgatccc gagacccagg accggatcct
     7321 cacgagcctg cagttcatgg cgcgtagcga ctgggaggcg caggagcggt tcctcgggga
     7381 gcacgcgaac gttcgcgagg cgctcggcga gcgcttcgcg gggacgtgga tccgatggga
     7441 gcggcaccag cgcgagtggc acgttgcggt ggtcgatcca accccagatg atgtcgaact
     7501 cgtggccgat gccgctcgcc gcgcggggtt cgttccgttc gtgcgcggcg tccggtactc
     7561 cgaagccgag gtcgagtcgt tccgccgggc cggcgaagcc gctctggatc ggatgcggtc
     7621 gagcccggcc catgatgtcc ttcgcggcta cggccccgac gccgaggcga acggtgtccg
     7681 cctgacgatc agcgaggcga acaccaccgt ggtggctgac ttgctcgccg tcatgccggt
     7741 ggacgtggtc gagatcgagg tcaacgacat ccgggccgtg gcgctctgag ggccgcggtt
     7801 ccccatccgc tcaaggcgcc ggagggtcga agggcgacga gacctcactt cgcggccatt
     7861 caaggaggtt ccctccacgt ggaacgatgt tcgcggatcg agatggtgct cgtcagcctt
     7921 aggatggcgc cgtggcgatg tcgcgagaga caaagccggg caagatcgga gcctggatcg
     7981 cgcttggcct gagtatcgcg ctcgccgtcc gcgtcatcgc gatcgacaat cccgaagcgc
     8041 tcgtgatcgt cgcccctgcc ttggtgggtg ctctcctggc ggtcgcctgg ccgcagatgc
     8101 gagtggctct gctgacgggc ctggtattga tcggagtaac agcaaccttc tcgctgatcg
     8161 gctggatcgg gctactcttc atcccctcga tggtcctaat cggcctgtcc gcctatcgca
     8221 tctcccgaac gccgaccctc cagccgtaac atcgcctcat cgctgggtgg ggactcgcgg
     8281 ttggaatccg gatgaggaac gtgcccgcag atgccctccg aatcgagatc caccccccgc
     8341 cggatgggcc ccgctctgaa tgcgaccgac cgcctcggca ttggaggtcg ctcatcccac
     8401 tgatggttga atcccttcag ctcgcccgac ggttcacggc ccaagggaat cagacgacca
     8461 ctgccacgtg gtcgcctcat ctcgcagacg ggcttcccag gcctagccaa ctcggtgtga
     8521 ccccgtggat ggcgccgcag gggtcccgtc ggggtcccgt gaacgaccgc gaaacgacgc
     8581 aagacgaccc cattgcacgc gaacgataat gtccagctca cgggccggat ggcggcgacg
     8641 ccgcaggtca gaggaccgga ccctgcaacc tttgccaagg tgggggtcgc gggttcgaat
     8701 cccgtcgtcc gctccaagaa gtggtgcggt gaccagcgcc ttctctgtcc gctgccgatc
     8761 cgccgtcacg cgcctgccca tgagtcgccc atggcttgcc catgaccgta ggcgtagagt
     8821 ccggcgatca tgggacaggg atccatgcgc cggcgtggca aggacacctg ggagctacgc
     8881 gtctaccagg gcatcgaccc cgatactcgg cggcagcgat ggctgaccaa gacggtccac
     8941 ggctccgatc gcttcgcccg ccgacagctt gaggagctgg tggccgaggc cggtcgggct
     9001 cggatacgag ccggcacact ctctggtctg ctcgaccagt ggctcgaggc cgcctctccc
     9061 ggctggtcgg cctcgacagt gagccacacc cgctccatcg tcgactgcca cctcaagccg
     9121 cacgtcggcc acctcgacct ggccaagctc atgactgccg atatcgacga cttctacgcc
     9181 cacctcctgc gagccggcgg ccgggacgac cggccgctcg cccacggaac cgtcgcccgc
     9241 gtccacggcg tgcttcaccg cgcttaccca agccgtcagg tgggactgga tctggatgaa
     9301 tcccgcgtcc aacgcgacgc cgcccagagt ggccccggcc gagattcgtc cgccgagccc
     9361 ggagcaggtc gccgtcctgc tcgagtgggc gaagcgtcgg gaccccgctc tctactgctt
     9421 cctccggctg gccgtctgga cgggtgcccg tcggagccag ttgctggcgc tgcggtgggg
     9481 cgacgtcgac caggaacgag ccgcgatcgc cttcagccgc gcactggtcg aggggccgga
     9541 cggacccgag cttcgctcca cgaagaccca ccggacgtac cgcgtcgagc tcgacgccga
     9601 gtcactcgac gtgctaacgg cgcaccgaca ctgggcgagg acgaaagccc gggacgcggg
     9661 cgcggagctg acggcgggtg cgtttgtatt cagttctcgt ccagaaggcg cgaagccctg
     9721 gctgcctaac tgggtgacga agcggttcat cgatgcccgg cgtgccgcgg ggttgccgca
     9781 cttccggctc catgacctgc gccacttcat ggcgagcgag atgcttgcgg cgggcgttcc
     9841 catcgtgacc gtgtcccagc gcctcagtca cgctcgcgcg tccacgacct tgaacgtcta
     9901 cgcgcactcg gtccccggag gagatcgccg agctgcagag acgctggcgg taattctcgc
     9961 ggccggccgg gggcacgcgg accggtctga tggcagaaca acgagacggt tcagggggac
    10021 ggccgagcgt gatggaggca ccgtcggcta actggccgag cggcgggtgg gccgcggtag
    10081 gctgccccat cgctcgggcg acgccatctc catggtgctc gagcaccccc gttcttgtct
    10141 gagcgtctgt gttctcggtg agcaacttga caaatcggag acaaacggat gaaaatccac
    10201 acgaatccac cccaagggga gaggccactt gtacgcggtg gagcggcgcc aggagatcgc
    10261 cactctcatc gaggagcgcg gtcgcggcga cgtcaccgag ctggcagacc gcttcgacgt
    10321 gacacccgag acgatccgtc gtgacctgac cgagctcgaa cagcgtcggc tccttcggag
    10381 ggtccacggt ggagcgatct cgatccaccg gttccgttcg gagcccgcga tcgccgagaa
    10441 ggcggaagcc atggccgacg agaaggcggc gatcgcggcc gccgcgacgg cattcgtccc
    10501 ggaagccggc tcggtgctga tcgacgcggg cacgtccacg ctcgcgctcg ctcggatctt
    10561 ccccgagcgc gagctcaggg tgttcacgca ttcggtcgcg gtcgccctgg agctcgcgtc
    10621 caagctcgac ctccggctgt acgtgatcgg cggacgcctg cggacacgaa ccctcgcgaa
    10681 cgtggacgag tgggcgctcc gtcagctcgc agcccttcgc gtcgacgtcg cgttcgtcgg
    10741 gacgaacggg ttcagcgcgg agcgcggtct ctccactccc gaccccgccg aggcggccgt
    10801 gaagcacgca ctctgcctgg ccggcgcgca gacggtggtg ctcgccgacc actcgaaggt
    10861 gaaccaggag tcggcggtcc ggttcgccgc gatcgacgag ttcgatgtct tgatcagcga
    10921 ccacgggctc gcggagccct atcgccgcgc tctcgaccag gccggcgtgg aggtggtact
    10981 cgcctgaggg gatgagcaca ggagcaaggt gcgggggctc gcgtggcgcg agacccgcag
    11041 gagtactcgc ggagccagag agcggagcca gagaaagggg gagcacgatg gcaacagcga
    11101 tgccggtccc cgcaggggcg accggaggat ggcgggcgag ggtccagcgg ttcggtggat
    11161 tcctggcagg gatggtgatc cccaacatcg gcgcgttcct cgcgtggggg ctcatcacgg
    11221 ccttcttcat cccgacggga tggatcccca acgaggatct cgcccagctc gtcgatccca
    11281 tgatcttcta cctgctgccc atcctgatcg gcttcacggg cgggttcctg atccacggac
    11341 accgcggcgg cgtcgtggga gcgatcgcca ccgcgggcgt tgcgatcggg gggtcggtcg
    11401 gaaccgaggt cgggggcgaa ctggtccgga ccccgatgtt cctcggcgcc atgatcatgg
    11461 gcccgctggg gggttggctg atcaagcagt tcgaccaggc ggtgatgcat cgggtccgga
    11521 ccgggttcga gatgctcgtc tcgaccttct cggccgggat cctcggggcg atcctcgcgg
    11581 tcctcgcgta cttgctgatc ggaccgccgg tctcggtgct ctcgaacgcc gccggtaacg
    11641 ccgtggagtt cctggtcgac aacacgctgc ttccgctcac gtcgatcatc gtcgagccgg
    11701 caaaggtcct gttcctgaac aacgccatca accacggggt cctggcgccg ctcggcgtcg
    11761 cggaggccgc cgaggagggg aagtcgatcc tgttcatgat cgagacgaac cccggtcccg
    11821 gactgggcct gctactcgcg tactggatgt tcgggcctcg tttgctgcga ccctcggtgc
    11881 ccggcgcgat catcattcag ttcgccgggg gcatccacga gatctacttc ccctacgtgc
    11941 tgatgcgtcc gatcacgatc gtgggcgtga tcgccggcgg catggcgggg gtgttcacgt
    12001 tcctcgcgac cgattcgggg ctcacggcga caccttcgcc gggcagcatc ttcgcgtacc
    12061 tggccgtcac cccgaagggt ggccttcccg gggtgctcct ggggatcacg gttgcgaccg
    12121 cggtctcgtt cgcggtcaac gcgcttatcc tgaaggcgac cgtgcgcgaa caggacgagg
    12181 tcgtcatccc cgagattgtc ccgaccgggc cggcgccggc gaccgcatga tggcgtcggt
    12241 ccaggggagc gacgtccgga aggtcgtggt cgcctgcgag gcgggcatgg gcagcagcgt
    12301 gctgctcgtg acccagctcc gcgatcggct gaagggaacg ggcgccgagg tggtgcactc
    12361 gccggtgagc caggtccccg cggacgcgga cgtgatcgtg tgccatcggg ggctcgaggc
    12421 gaccgctcgc aagatcgctc cggagaaggt cgttgtgacc ttcaacatgt tcctcggcga
    12481 cccggcgttc gaccgggtcg tcgaggcgat cactgagggg aaggccctcg aggggtagcc
    12541 atggacgcgc ttcttcccgc cgccgggatc cgcctcggcc tgcaggcgtc ggatcgattc
    12601 gacgccgtcc ggcagtccgg ccaggtgctg atcgacatcg gcgccgtgaa ggcgccctac
    12661 gtcgacgcga tgctcgaacg cgagcggggg atctcgacct cgctcggtga ggggttcgcg
    12721 atcccgcacg ggacggagga gagccgtcgg tggatcaacg agacgatgct ctcgttcctg
    12781 cagttccccg ccggcgtcga gtgggacgac gtcatggtga ccgtctgcat cgggatcgcg
    12841 gccgtcggcg atgagcacgt gggggtgcta gcgaacctgg cgcgcgtgct gaccgtgcct
    12901 gagcaggcgg caagtcttcg ggccgcggag cggcccgaag acgtgctcgc gatcctcggc
    12961 cgggacgagg gggccatcgg gtgaaggtcg cccgtctcta cgcgccgggc gacctccgga
    13021 tcgaggacgc cgacgtcccc gaggccggtc cgggtgatct cgtgatccgg ggtcgcacct
    13081 gctccacatg cggtacggac gcgaagatct tccgcttcgg acatcaccac atctccttgc
    13141 cccgcgtcct cggccacgag gtggcgggcg aggtgacgga ggtgggctcg gaggtcggcg
    13201 ttcagggcgg ccacagatgg tcgccgggcg accgcgtgca gatcatcgcg gcgatccccg
    13261 acggggtgtg cttcctctgc cggcgggggc agcacacggt ctgcgaggaa ctcgagtcga
    13321 tcggctacca gtacgacgga gggttcgcgg agtccatgcg ggtgcccgcg aaggtgctcg
    13381 cggtcgacgg ggtcaaccgc atccccgagc acgtcccttt cgaggaggcg agcctcacgg
    13441 agccgctcgc atgcgtcctg aacggacagg agctcgccca agtcggcgag ggggatgccg
    13501 tcgtcgtcct cggcgcgggg ccgatcggct gtctccacgt tcgcctggca cgcgcgcgtg
    13561 gcgcccggat ggtcgtcctc atcgacgtga atcaggggcg gctcgacctc gccgctcgtg
    13621 cggagccgga cgcggcgatc gacagtacga aggacgatcc gatcgatgcg atcaagaagc
    13681 tgaccgacgg acgaggtgcc gacgtggtga tcaccgcgac cggcgtcggt caggcccagg
    13741 agcaggccct cgagatggcg gccctccgcg gccgaatctc cctcttcggc ggcctcccgc
    13801 gagacgactc ggtgatccgc ttcgactcca acctcgtcca ctaccgagag ctctccgtcg
    13861 tcggtgccta cggatccgcg ccccggcaca atcgggaagc gctcgccctg atcgccgacg
    13921 gcaaggtgcg ggtcgacgat ctgatcaccc accggatgcc gctcacggac gtcagccggg
    13981 cgatcgagac cgtgatgtcc ggcgagggcc tgaaggtcgt catcgagccg tgacgtcatg
    14041 atcgtcacgg tgacgccgaa ccccagtctc gatcgcaccc tcgaggtgga ggagctgcgg
    14101 cgaggagagg tcgtccgcgc ccacgccgat cgatcggatc cgggcggcaa aggcgtcaac
    14161 gtgtcgcgag ccctggcggc gaacgggtac ccgtcgcgcg cggtcctccc gctcggcggc
    14221 catgacggtg cgctgctcgc ctccctcctg gagggcctcg gaatcgaggt cgccgccgtc
    14281 ccgatcgccg acgcgatccg atcgaacgtc accgtggccg agcctgacgg gaccgtcacg
    14341 aagctgaacg cgcccgggcc gcggctgtct cccgccgagg tggatgcgct cctcgagcgc
    14401 gcggtcgcgg ccgcggacgg cgcgggctgg gtcgtggctt cggggagcct cgcgccgggg
    14461 acgcccgacg acctgtatgc gcgcctcgcg gccgcggtcc gacctgtggg tgtgcgcgtc
    14521 gcggtcgata caagtgggcc cgccctcgaa cgagcgctcg tggccggtcc cgacgtcgtg
    14581 aaaccgaacg aggaagagct caccgaggtg accggcgtgg tccccgagac gctcggggat
    14641 gtcgtgctcg ccgcgaatca gctccgcgag aagggggtgg ggacggtgct ggtctctctc
    14701 ggggctcgtg gggccgtgct cgtcgaggac ggcgaggccg tgcacgcgga gagcgaggtg
    14761 atcgtcccga ggagcaccgt cggtgccggg gacgcgctcc tcgcgggctt cctcgcggcc
    14821 ggtggcgaag ggcccgacgc cctcgccgaa ggcgtcgcgt ggggcgccgc cgcctgcgtg
    14881 ctcccgggga cagcggtgcc gagccccggc gatctccaac gacagctggt ccagacgcat
    14941 ccggtcgatc ccgagcggtc gttggcgctc ggtaccgacc ggcgccggag gtcgacccat
    15001 gcctgaggcg agggtgacgc tgcggaatcc cacggggttg cacgcgcgac cggcgaagat
    15061 cttcgcgaag gcggctgcgg cgttccccgc cgacgtggtc gtcgagaagg acggtcgccg
    15121 cgtgaacgcg aagtcgacgc tctcgctgct cacgctggac tgtcaccagg gcgacgagat
    15181 cctgatcagc gtggaggggg aggcagcgga cgagacgctc gcggagctcg ttggtctggt
    15241 cgaggcgggc atcggcgagc acgagggtgg aggagtcgcc acgtgagcac gaggctgtcg
    15301 ggtcgagccg cggcgcccgg cgccgccgtc gctcctgcct tcgtcctggc gccacaaccc
    15361 gtcctgacca agctccccga ggtcgcatcc ggcgctcccg aagatgagcg cacgcgcctc
    15421 ctcggggcgc tcgagcgggc ggaggccgag ctccgcgagc tcgccgaacg cgtcgctgcg
    15481 tcggccggcg aggaagaggg cgagatcttc gaggcccatg ccgagttcgc tgccgacccc
    15541 gagctggccc ggctgaccga ggaagccgtc cgagccggcg cctcggccga acgcgcggtc
    15601 gtcggcgcgt tcgagacgtt ccgggagctc ctggtcgcgt ccgcgagcga gtacctcgcg
    15661 gcacgtgcat ccgacctcga cgacgtccgt gaccgggtcg tgaagatcct cctggggatg
    15721 agcacgggtg gcgatcggcc ggagcagcgg tcggtggtcg tggcccacga gctcacgccg
    15781 tccgagaccg cgagcatccc ggtcgacctg atcgccgcga tcgtgacgga gaccgggtcg
    15841 ccgacgagcc acgccgcgat cctggcgagg gcgctcggtg tccccgcggt ggtggcgtgc
    15901 gacggagtgc tggcggccac gcgggcgggc gtccacatcg cggtcgacgg acgggccggc
    15961 gaggcgatcg tcgatccgac gcccgacgag cgcgcggaga tcgtccgtcg tcacgaacag
    16021 gaggagcggc gccgcgagga cctccgtgcc ctccgcgaga agccgggtcg gaccgcggac
    16081 gggcatccgg tggagctcgc cgccaacatc ggctctatcg accacatccc cgccgccgtg
    16141 gaggccggtg gtcaggggag cggtctcgtc cgcaccgagt tcctgttcat cgggcgggtg
    16201 accgcgccga ccgtcgaaga gcagacgaag gtctacgcag agatcctccg aggcttcccc
    16261 ggccaccgcg tcgtgtttcg gaccatggac gtgggggccg acaagccgct gccgttcgtg
    16321 gagcgcgacc ccgaggagaa ccccgcgctc ggtctccgcg ggatccgctt gagcttgcgc
    16381 agtccggacc tgttccgcga tcagctgcgc gccctcgtac gcgcacgagc ggacgtggcc
    16441 ggcgaggagg ccggtcgcct cgcgatcatg ttccctctcg tcgccacggc cggcgagctc
    16501 gaagcggcgc gaggagtgct tcggggtgtc gccgacgagg atggggtcga cctcgagggg
    16561 atcgaggtgg gcgtgatgat cgaggtgcca tcgggcgccc tcggcgctgc ccggctggcg
    16621 cggcacgccg acttcttctc gatcggcacg aacgatctga tccagtacct cttcgccgtc
    16681 gaccggctga acggtgccgt cgccgacatc ggcgacgtcc tcgagcccga cgtcctcacg
    16741 ctgatcggga gcgtcgtcga agcgggacac gcgaacgagg cctgggttgg tgtgtgcggt
    16801 gaagcggcag gtgatcccac ggtcgcgggc gcgctcgtcg ggctgggcgt cgacgagctc
    16861 tcgatgacga aggtcgcgat ccccgaggtg aaggacgcgc tccggcgact cacccgtgac
    16921 gaatgccgcg aggccgtcca cgccgcgatc gccggtgccg cggacgccgc cgggtcgcgc
    16981 acgatcctcg aggaacggct ccgcacgtcg ctcgcctgac cggcggccca tcgatggggc
    17041 cacctcacct cgcgcgaacg ccgggggatc gagtaattcc cttcgcggac agtggtgagc
    17101 acacgataaa ggcgatcatt cagaaccgct acgggtcgcc ggaggtcctg cagccggcga
    17161 gatcgccgag ccggtcgcat gcacatgtac gaccaacagc tcgtccgtgt gcatgcgacg
    17221 tcgatccacg ccgacgtctg gcacatgatg cggggcctgc cgtacgtcat gcgattcatg
    17281 ggcgccggat tgcgggggcc gaaggacgtc gtgccgggta cggatctgtc gggagtcgtc
    17341 gaacgggtcg gcaagggcgc gaggcgcttc caaccgggcg atgaagtgtt cggacagacc
    17401 ctcgtcgcca acctctggcg ccacggggcc gttcgccgaa cacgccgccg ttccggagtc
    17461 gaggttcgag cggaagccgg cggccctcac gttcgaacag gccgcagccg tcccacgtcg
    17521 ggatcgctcg ccgtgcaggg cgttcgcgac gaggggcgga tccaggcggg acatcgcgtc
    17581 ctgatcaacg gcgcgggcgg tgccgtgggg acgttcgccg tgcagctcgc caaggcgctc
    17641 ggggctgaag tgacggccgt ggacgctgcc gagaagctcg agatgctccg ggcgatcggt
    17701 gcggatcggg tcgtcgacta cgggcagaag gacttcacag gagccacacg cgacgggcac
    17761 gttcccgaga cggtctgaag acaggtctgt gatgcgacgg tgttcggatg gacgcggccg
    17821 aatcaggccg tctactgcag ttctagtggt gggcccccgg gactcgaacc cgaacctgcg
    17881 gattaagagt tgtcgaaccc ccggccgcgc ctacgtcggc gggaccggat ggtgatcgcg
    17941 gcgttggcgg atctgatcga ccgcgaccgc tggagtgggt tcatcgtgtc cccggcgacg
    18001 atccttcggt ggcaccggga cctcgtcagg cgcaagtgga cgttccggca ccgcaaggct
    18061 ggccggccgc cgctcgattc cgcgctggtt cgtctgatcg tgcagatg
//
LOCUS       DATBRK010000103         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2467323, whole genome shotgun sequence.
ACCESSION   DATBRK010000103 DATBRK010000000
VERSION     DATBRK010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467323"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="VIH70_03185"
     CDS             complement(<1..1509)
                     /locus_tag="VIH70_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8115397.1"
                     /translation="MLRRSAAPVRLVPLILALALTAAACGGGTDEPTQGNGPPATGGT
                     PVGGTLKLAMQSDVQEAFDPQKEYYAVAWAFDRCCLLRTLLSYNGKTTAEGGAEVQPD
                     LASALPEQSADGLTWTFTLKPGIAYAPPLDDVSVTAQDFIRAMERTACGTCSTGGYGF
                     YYSAIEGFDEFAAGNADSISGLEAPDDSTLIVRTTTPTGDLPFRMAMPAAAPIPPLPG
                     DPKARLGIATGHDDNFGRFLIGTGPYMFEGTDLLDFSLPPGDQEPVAGYDVGRSIVLV
                     RNPSYDSATDDLREGYVDRIEVTIGGDSNDLALKVDAGELDMVYDGVPPAAQIRQYAS
                     NPDLQDQIHSDPSDGVRYIEMNLATPPFDDQNVRIALNYAVDKEGFRQLRGGPLFGEL
                     AGHIMVNSLQGDQLRDYDPYSSPNGRGDATAAKQAMAASRYDTDGDGVCDDASCDGIV
                     TIIDEADPYPKQTALLQQSLEPLGITLDVKTFERTTMYAKCEDPNSQWGLCPS"
     gene            complement(1563..1955)
                     /locus_tag="VIH70_03190"
     CDS             complement(1563..1955)
                     /locus_tag="VIH70_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8115398.1"
                     /translation="MRKHAEQVRAYLEANLTQDLDMNAVARAASLSPFYLTRIFKQRY
                     GIPPYRYLIGLRIRYASDLLRDSSLSVTQVCHRSGFNSLSHFITTFRNHTGMSPSQYR
                     RVVDWEQDAGRFGQETEALVETPRRMNF"
     gene            2330..2403
                     /locus_tag="VIH70_03195"
     tRNA            2330..2403
                     /locus_tag="VIH70_03195"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2364..2366,aa:Ile,seq:cat)
BASE COUNT          367 a          792 c          839 g          413 t
ORIGIN      
        1 gctcgggcac aggccccact gcgagttggg gtcttcgcac ttcgcgtaca tcgtggtgcg
       61 ctcgaacgtc ttcacgtcca gcgtgatgcc gagcggctcg aggctctgct gcagcaacgc
      121 cgtctgcttg gggtacgggt ccgcctcgtc gatgatcgtc acgatgccgt cgcacgacgc
      181 gtcgtcgcac acgccgtcgc cgtccgtgtc gtacctcgag gcggccatcg cctgcttcgc
      241 ggccgtcgcg tcacctcgac cgttcggcga cgagtagggg tcgtagtccc tgagctggtc
      301 gccctggagg ctgttcacca tgatgtgccc cgccagctcg ccgaacagcg gaccgccacg
      361 gagctggcgg aacccctcct tgtcgaccgc gtagttgagc gcgatccgca cgttctggtc
      421 gtcgaacggc ggcgtcgcaa ggttcatctc gatgtagcgg accccgtccg agggatccga
      481 gtggatctgg tcctgcaggt ccgggttgct ggcgtactgg cggatctgcg ccgcgggcgg
      541 cacgccgtcg tagaccatgt cgagctcgcc cgcgtccacc ttcagcgcga ggtcgttcga
      601 gtctccgccg atcgtgacct cgatgcggtc cacgtagccc tcgcgcagat cgtcggtggc
      661 cgagtcgtag gaggggttgc ggaccaggac gatcgaacgc ccgacgtcgt agcccgcgac
      721 gggctcctgg tcgccgggcg ggagcgagaa gtcgagcagg tcggtcccct cgaacatgta
      781 gggcccggtc ccgatgagga atcggccgaa gttgtcgtcg tgcccggtgg cgatgccaag
      841 ccgggccttc gggtcgccag ggagcggcgg gatcggcgcc gcggcgggca tcgccatccg
      901 gaacggcagg tcgccggtcg gcgtggtcgt gcgcacgatc agcgtcgagt cgtccggagc
      961 ctcgagaccg ctgatcgagt cggcgttacc ggcggcgaac tcgtcgaatc cctcgatggc
     1021 cgagtagtag aagccgtatc cgcccgtcga acacgtaccg cacgccgtcc gctccatcgc
     1081 gcggatgaag tcctgggcgg tcacggacac gtcgtcgagc ggcggtgcgt acgcgatgcc
     1141 cggcttgagt gtgaacgtcc acgtgagccc gtccgccgac tgctcgggga gtgcggacgc
     1201 gagatcgggc tggacctcgg caccgccttc ggccgtcgtc ttgccgttgt acgagagtag
     1261 cgtccggagc aggcaacagc gatcgaacgc ccacgcgacg gcgtagtact ccttctgcgg
     1321 gtcgaacgct tcctggacat ccgactgcat cgcgagcttc agcgtcccac ccaccggcgt
     1381 cccgccggtg gcggggggcc cgttgccttg cgtcggctcg tccgttcctc ctccgcacgc
     1441 ggccgccgtg agggcgagcg cgaggatgag cgggacgagc cgaacgggtg ccgcagatct
     1501 cctgagcatg ccgtcccctt tcggcccgag acatgcggcc gcggcgggac gccgggccgc
     1561 tctcagaagt tcatacggcg gggcgtttcg accaacgctt cggtctcctg cccgaacctt
     1621 cccgcatctt gctcccagtc caccacgcgt cggtactgcg agggactcat gccggtgtgg
     1681 ttgcggaagg tcgtgatgaa gtgcgagagc gaattgaacc ccgaccggtg gcagacctgc
     1741 gtcacgctga gcgatgagtc gcgcagcagg tcggacgcgt agcggatccg gagcccgatc
     1801 aggtaccggt acggcgggat gccgtagcgc tgcttgaaga tgcgggtgag gtagaagggc
     1861 gacagggacg cggcgcgcgc caccgcgttc atgtccaggt cctgagtcag gttggcttcg
     1921 aggtacgcac ggacctgctc cgcgtgtttg cgcacgtgtc ggctctggtg gttgccgtcg
     1981 ccgaacatcc ggaacaggct cgaccccccc tctctcctcg aagtgtacgc gggggagtgc
     2041 tcgcggcgtc ttgcggattt ctgcccatcc ctcggccgcc tctgggctcg tgcctggttg
     2101 tgggcccacg cgggctcgaa cggtggctcc caagatggcg gtggtgccag cagggtgacg
     2161 ggacaccgag gaaccggacg ccggtccgct caaggaacgg gaccgcctcg acgagacctg
     2221 atttggatct cggcgacgct tcgaggacgg gacgatcgca ggggaaacga ccgtgatcaa
     2281 caacccgatt gacacctaca cccgagcgct acgaatacac tccggaatcg gggcggtagc
     2341 tcagtcggtc agagcagcgg actcataatc cgtcggtcgc aggttcaagt cctgcccgcc
     2401 ccacgagaga a
//
LOCUS       DATBRK010000104         5723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2467324, whole genome shotgun sequence.
ACCESSION   DATBRK010000104 DATBRK010000000
VERSION     DATBRK010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467324"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VIH70_03200"
     CDS             complement(<1..804)
                     /locus_tag="VIH70_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115399.1"
                     /translation="MKPTDDQATPEASGVSEAKDTARRRGTWITRYKPDLSVRFFSSS
                     ADASRARRPTDLVLFLLAAVTLGLVSLFAPDPTAADARFTRLVEDLPGLLGWFWEIAS
                     DMLVIWPLVLLVAAVVASRRLFLLRDQLVGAFLTVGVTTLVSGGFSGLVDGVTATGAP
                     PVYPAVRVAFAAALIATTAPHLGRPVRRLGRWVILLGSLGVVALGISLPLGVVSALAI
                     GFGSAALVHLAFGSPGGLPSMEQVRAALEELGVAAVDLRVAELQPKGVAL"
     gene            complement(895..1275)
                     /locus_tag="VIH70_03205"
     CDS             complement(895..1275)
                     /locus_tag="VIH70_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY8115400.1"
                     /translation="MLAYEWTFGSVLWAMVVFFFWFMLIWIFIGVFADLFRRNDLSGW
                     AKAGWLLLIFILPFFGILVYLIARPKMTEQDKEMMAAISERERRATGYSAADEVAKLA
                     KLRDEGKITAEEYERMKQQAMMQV"
     gene            1389..3059
                     /locus_tag="VIH70_03210"
     CDS             1389..3059
                     /locus_tag="VIH70_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8115401.1"
                     /translation="MQSNGSDATPTRPPSDRPVARLANAGWRRRPSGEPPPLPREPGW
                     RAWGWAAVALLVAGVGIGLAIVPTDAQPPLLRWFEDLRTPTLVDVAKVLDGLTDPLVV
                     WILRVVLLVVAAFFARWRHLITLLALYVVVDFLDTILAVQRPAPEGVTVLSSNATTSF
                     PSLPVAALAVTLFGAAMMLAPAGRPRRLAQAGAFALTGLVVLARLLLGADYPVDAVYS
                     LLLGWIAVGLAFAFFVPDDVFPVSYARGGKAAHLDLGGARGQAIVAAVRDQLGFAVTE
                     IEPFGLAGSGGSSPLRMRVAELDGYLFGKIYSTGHLRADRWYKVGRSILYGQLEDEVP
                     LGSVRRLALYEDYALRLLRDVGIGVATTYGIVELTPDREYMLVTEFFSNATTLGDAEV
                     DDAIIDEGMELVRLLWASGLAHRDLKPANMLVRGGHLQLVDVSGLQVRPTPWRQAVDL
                     ANMMLTLALRTDPDRVYARAITFFSPDDVAEAFAAARGLAIPTQVSEKLKQDGRPILE
                     RFRELAPDRPPVSIQVWSVRRVALTVAAIVGALALAAMVWATLAIGLD"
     gene            3141..3404
                     /locus_tag="VIH70_03215"
     CDS             3141..3404
                     /locus_tag="VIH70_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY8115402.1"
                     /translation="MYLILALLAIALAAGWIAQVIVGRSHGRTDWTEALVAGFLGSFV
                     GGLLASLIAGDGLRFRPSGLIGSILGAIIVTAVWGLVRGRRTA"
     gene            3583..4002
                     /locus_tag="VIH70_03220"
     CDS             3583..4002
                     /locus_tag="VIH70_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8115403.1"
                     /translation="MQAGTFATVPAVSALALVAGRGRLAASLAVGGTIAWVGAKLAKP
                     FGGRARPRGVLPDVTIREGIEGDLGWVSGHTAVATTLAFVAAGELPRTARPILAGIVG
                     TVGFGRMYVGAHLPHDVVGGAGLGLMISAVIPPADGG"
     gene            complement(4004..5014)
                     /locus_tag="VIH70_03225"
     CDS             complement(4004..5014)
                     /locus_tag="VIH70_03225"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY8115404.1"
                     /translation="MEDVVDTQPPAPGSEFPPGLPRTEPAEPHASSTAPTGKDVEAPS
                     QAGRVARARRSAAELERRARREFDHAKEQHVAVRLAVRAFESDRRRAGGLLAGGLAYR
                     IFLWQIPLALFLVSALGLATELSGDDPADLARKTGMTAALAGAISQAVAASDSARWWL
                     LILGAFLTVWAGRGVYRGFRLVSELAWGIRAPRASALKGSLAVTGFGLLAVALQAFLP
                     KISQTLGLSVLVRFVLGLILASSLAFAVLRLLPRADVPWTSTLPGAVLVGVGVRLLGL
                     AVSTYFAYRLDHSNDLYGALGLAVVMMLFLFLVARLFVAAQFLNSTLHRRREPLPSAD
                     GG"
     gene            5109..>5723
                     /locus_tag="VIH70_03230"
     CDS             5109..>5723
                     /locus_tag="VIH70_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115405.1"
                     /translation="MSTPNERTTVDEGAASPERFGRVVPLRVAAQPPPPLRELVEQSF
                     RIAIGATALASEVLAEAVARTLGREPFAEGETPGDETARPASGVPLVAGAAIGVALEG
                     ARWGARAATTMLSSAELFLSLLTSPGFVQEPLRQAGERLGALDARWRQQRPRDEEAAA
                     SFLRLVVPQVVDAALDQIDLNELVAERIELDQVIDRLDLDRAIAR"
BASE COUNT          829 a         2084 c         2026 g          784 t
ORIGIN      
        1 gagcgcgacg cccttgggtt gaagctcggc cacacgcaga tcgaccgccg ccaccccgag
       61 ctcctcgagc gccgcacgga cctgctccat cgagggcagg ccccccgggg atccgaacgc
      121 caggtgcacc aacgccgccg agccgaaccc gatcgcgaga gccgagacca cacccagcgg
      181 aagcgagatg ccgagcgcga cgacgcccag cgacccgagc aggatcaccc atcggcccag
      241 gcgccggacg ggtcgcccga ggtggggcgc ggtggtcgcg atgagggcgg ccgcgaacgc
      301 aacgcggacg gcgggataga ccggcggcgc cccggtcgcc gtcaccccgt ccacgaggcc
      361 cgagaaccca cccgagacca gggtcgtcac acccacggtc aggaacgcac cgaccagctg
      421 atcccgcaac aggaacagcc gacgcgatgc cacgacggcc gcgaccagca ggaccagcgg
      481 ccagatgacc agcatgtcgc tggcgatctc ccagaaccac ccgaggagcc ccggcagatc
      541 ctcgacgagc ctggtgaagc gcgcatcagc cgccgtcggg tcgggtgcga agagcgagac
      601 caacccgagg gtgaccgcgg cgaggaggaa caggaccagg tccgtcggcc ggcgggccct
      661 cgacgcgtcg gcgctggagg aaaagaagcg gacgctgagg tcgggcttgt accgggtgat
      721 ccaggtcccg cgccgccggg cggtgtcttt cgcctcggac acgccggacg cctcgggcgt
      781 tgcctggtcg tccgtcggct tcaccgaccg cacccctccg aacgctcgag agccgccggg
      841 tcccggtcgt ccagggagcc ccagcggccc tcggagcttc gacgaacgat ggagctagac
      901 ctgcatcatc gcctgctgct tcatccgctc gtactcctcc gcggtgatct tgccctcgtc
      961 gcggagcttg gcgagcttgg cgacctcgtc ggcggcggag tacccggtcg cccgccgttc
     1021 ccgctcggag atcgcggcca tcatctcctt gtcctgctcg gtcatcttcg gccgcgcgat
     1081 caggtagacc aggatcccga agaacggaag gatgaagatc agcaggagcc agccggcctt
     1141 cgcccatccg gagaggtcgt tgcgtcggaa gaggtcggcg aacactccga tgaagatcca
     1201 gatcaacatg aaccagaaga agaagacgac catcgcccac agcacactgc cgaacgtcca
     1261 ctcgtacgcc agcatccgac cctccccttg ttggcccgct ctcgggcgcc ggcggagcgt
     1321 accccaagac gggcggggtt gcatccgcgc tgaaccgacg gcggggcagg ggctagagtc
     1381 cgttcccgat gcagtccaac ggatccgacg cgacgccgac acgcccgccg tccgatcgtc
     1441 ccgtcgctcg gctcgcgaac gccggctggc gccggcgccc ctcgggcgag ccgccgccgc
     1501 tgccacgcga gccggggtgg cgcgcctggg gatgggcggc ggtcgccctg ctcgtcgccg
     1561 gcgtcgggat cggtctggcg atcgtcccca cggacgcgca gccaccgttg ctgcgatggt
     1621 tcgaggatct gcggaccccg acgctcgtcg acgtcgcgaa ggtcctcgac ggcctgaccg
     1681 acccgctggt cgtctggatc ctccgcgtcg tgctcctcgt cgtcgcggcg ttcttcgccc
     1741 ggtggcgcca cctgatcacg ctcctcgcgc tctacgtcgt ggtcgacttc ctggacacga
     1801 tcctcgccgt ccagcgaccg gcgcccgagg gcgtgacggt cctgtcgagc aacgccacga
     1861 cgagcttccc gtcgctgccg gtggccgcgc tcgcggtgac gctgttcggg gcggcgatga
     1921 tgctggcccc ggccggtcgg ccgcgccggc tcgcgcaggc cggtgcgttc gcgctcacgg
     1981 gcctggtcgt gctggcgcgg ctgctgctcg gtgcggacta cccggtcgac gcggtgtact
     2041 cgctgctgct ggggtggatc gcggtgggac tcgcgttcgc gttcttcgtc ccggacgacg
     2101 tcttccccgt ctcctacgcg cgcggcggga aggccgcgca cctggatctc gggggcgcga
     2161 ggggacaggc gatcgtcgcg gcggtccgcg accagctcgg cttcgcggtg accgagatcg
     2221 agccgttcgg actggcgggc tccggcggat cgagcccgct tcggatgcgc gtggccgagc
     2281 tcgacgggta cctgttcggc aagatctatt ccacgggcca tctgcgcgcg gaccgctggt
     2341 acaaggtcgg gcgctcgatc ctgtacggac agctcgagga cgaggttccg ctcgggtcgg
     2401 tgcgccggct cgcgctctac gaggactacg cgctgcgcct gcttcgcgac gtcgggatcg
     2461 gcgtcgcgac cacctacggc atcgtggagc tcaccccgga ccgcgagtac atgctcgtca
     2521 ccgagttctt ctcgaacgcg acgacgctcg gcgacgccga ggtcgacgac gcgatcatcg
     2581 acgaagggat ggagctcgtg cggctgctct gggcgtcggg gctcgcccac cgggacctga
     2641 agcccgcgaa catgctcgtg cgtggcggtc acctgcagct cgtcgacgtg tccggcctgc
     2701 aggttcggcc gacgccgtgg cgccaggccg tcgacctcgc gaacatgatg ctgacgctgg
     2761 cgctccgcac cgaccccgat cgcgtgtacg cccgcgcgat cacgttcttc tcacccgacg
     2821 atgtcgccga ggcgttcgcc gcggcccggg ggctcgcgat cccgacccag gtgagcgaga
     2881 agctgaagca ggacggacgg ccgatcctcg aacggttccg cgagctcgcg cccgatcgtc
     2941 ctcccgtctc gatccaggtg tggagcgttc gacgggtggc cctcaccgtg gccgcgatcg
     3001 tcggcgccct ggcgctcgcc gccatggtgt gggcgacgct cgcgatcggc ctggattgac
     3061 gcgcaaagcc cgcgtgggaa cgggacagca ccccggccgg gctcgcgtac actgcaccga
     3121 ccttcgaacg ggagaagccg atgtacctga tcctcgcact cctcgcgatc gcgctggccg
     3181 caggctggat cgcacaggtc atcgtcggcc ggagccacgg ccggaccgac tggaccgagg
     3241 cgttggtcgc cggcttcctc ggctccttcg tgggcgggct gctcgcgagc ctgatcgcgg
     3301 gtgacggcct gaggtttcgg ccgagcggcc tgatcgggtc gatcctcggc gcgatcatcg
     3361 tgaccgccgt gtgggggctg gtgcgcggtc ggcgcaccgc gtaacgctcg gccgcggcgc
     3421 accgtccgga cccggcggct cggtcggttc gcgctcgggg cggccctgtt cctcgtaagc
     3481 ctcgccgagg cccggcgggg caggctcccc gactacgagg aacgcgtctt ccggaccgtc
     3541 aacggcgcga gcgaccgaat ccgcgtcccc gttcgcgcga tcatgcaggc cgggacgttc
     3601 gcaacggttc cggcggtctc ggccctcgcg ctcgtggccg gccgcgggcg ccttgccgcg
     3661 agcctggccg tgggcggcac gatcgcctgg gttggcgcca agctcgccaa gccgttcggg
     3721 ggccgggccc ggccgcgggg cgtcctgccc gacgtcacga tccgcgaggg gatcgagggc
     3781 gacctcggct gggtctcggg ccacaccgcc gtcgcgacca cgctcgcgtt cgtcgcggcc
     3841 ggggagctcc ctcggaccgc gcgacccatc ctcgcgggga tcgtggggac ggtggggttc
     3901 ggtcggatgt acgtgggcgc gcatcttccc catgacgtcg tcggcggggc gggccttggg
     3961 ctgatgatct ccgccgtgat cccgccggcc gacggcggct gagtcaaccc ccgtccgcgc
     4021 tcggcagcgg ttcacgccgc cgatgcagcg tcgagttcag gaactgggcc gccacgaaca
     4081 gccgcgcgac caggaacagg aagagcatca tcacgacggc gagcccgagc gccccgtaca
     4141 gatcgttgct gtggtcgagg cggtaggcga agtatgtcga cactgccagc cccagcagcc
     4201 gcaccccgac gcctaccagc acggcacccg gaagggtcga ggtccagggc acgtccgcgc
     4261 gcggcagcaa ccgcagcacc gcgaacgcca acgaggaggc caggatcaga ccaaggacga
     4321 accgtacgag gacggagagc ccgagcgtct ggctgatctt cggcaggaac gcctgcaggg
     4381 cgacggcgag caaaccgaag ccggtgacgg ccagggaacc cttcagcgcg ctcgcgcggg
     4441 gagcgcggat gccccacgcg agctcgctca ccaaccgaaa ccctcgatac acaccccgcc
     4501 ccgcccagac cgtcaagaac gcaccgagga tcaggagcca ccaccgcgcg ctgtccgacg
     4561 cggcgacggc ctgagagatc gctccggcga gggccgcggt catgccggtc ttcctcgcga
     4621 gatcggcggg atcgtccccc gagagctcgg tcgccaggcc gagggcgctc acgaggaaca
     4681 gcgcgagcgg gatctgccac aggaagatcc ggtaggcgag tccccccgcg aggagaccac
     4741 cggcgcgtct gcgatcgctc tcgaaggccc ggaccgccag ccgaaccgcg acgtgctgtt
     4801 ccttcgcgtg atcgaactcc cgcctcgccc ggcgctcgag ctccgccgcc gagcggcgtg
     4861 cacgtgcgac ccggcccgcc tggctcgggg cctcgacgtc cttcccggtc ggggcggtcg
     4921 atgacgcgtg cggctcggcc ggctcggtcc ggggcaggcc ggggggaaac tcggatcccg
     4981 gggcgggcgg ctgcgtgtcc acgacgtcct ccacgcatcg gcacgtgatc ggcccggacg
     5041 acgttcgggc acggcgatgg tagcccagcg atgagcaccg ttggcacctc gtcacccgag
     5101 gggtgatgat gagcaccccg aacgagagga cgaccgtgga cgagggcgcc gcttcacccg
     5161 aacgtttcgg cagggtcgtc ccgctccggg tcgccgccca gccgccgccg ccgctccggg
     5221 agctcgtcga gcaatcgttc cggatcgcga tcggggcgac cgcgctcgcc tccgaggtcc
     5281 tcgccgaggc cgttgcccga acgctgggcc gcgagccgtt cgccgagggc gagacgcccg
     5341 gcgacgagac cgctcgaccg gcctccgggg tcccgctcgt cgccggcgcg gcgatcgggg
     5401 tcgccctcga gggcgcccgg tggggcgctc gcgccgccac cacgatgttg agcagcgccg
     5461 agctgttcct ctcgctcctg acgagcccgg ggttcgtcca ggaaccgctc cgccaggccg
     5521 gggagcggct cggggcactc gacgcccgct ggcgtcagca acgcccgcgc gacgaggagg
     5581 cggccgcatc gttcctgcgg ctcgtggtcc cccaggtcgt ggacgcggcg ctcgaccaga
     5641 tcgacctcaa cgagctcgtc gccgaacgca tcgagctcga ccaggtgatc gaccggctcg
     5701 acctcgaccg ggcgatcgcg cga
//
LOCUS       DATBRK010000105         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2324443, whole genome shotgun sequence.
ACCESSION   DATBRK010000105 DATBRK010000000
VERSION     DATBRK010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2324443"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VIH70_03235"
     CDS             complement(<1..960)
                     /locus_tag="VIH70_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEY8115406.1"
                     /translation="MSEHQSTPREDGFSMPAEWEPHEACLMEWPTRTRKELWGDRFEE
                     AERDYATVANAIAAFEPVVMVCDPEQQTEVRRRCGDGVEVLPLPIDDSWMRDNGPIFV
                     RDAAGRIALVHFGFNSWGEKYLPYDRDAQVPRLIAEHLGMRRYEAPFVLEGGSFFVDG
                     EGTLVTTEQCLLNPNRNPTLSREQIERGLRDYLGVEAIVWLEFGHSTDRDTDGHIDGI
                     APYLAPATVALLLPEDPADPDHERGRANLDRLQSTRDARGRAFEVIAFETRPPGRVPY
                     LNFYLANNGGLVMPIAERPEDEQALEQIAKMLPDREVVPVPGNC"
     gene            1099..1542
                     /locus_tag="VIH70_03240"
     CDS             1099..1542
                     /locus_tag="VIH70_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115407.1"
                     /translation="MAKLEGIRSLDLILEEVRSERDLQLSHFDAIDTKAGIVLGFAGA
                     LVALAPRGAELLLDLGRALAVACGFTCLSAFWPRRYWSTNLRPFRDKYLAAELEFTKI
                     HLLDTQIGMADRMSGILASKAMRLKLAMIALALAVFLTAVGVVLD"
     gene            1557..1718
                     /locus_tag="VIH70_03245"
     CDS             1557..1718
                     /locus_tag="VIH70_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115408.1"
                     /translation="MEKEPNADHGGEATKPEETPPPFEPDPDIVTFLERGRRQDPKRT
                     WEATEPAER"
BASE COUNT          301 a          638 c          667 g          315 t
ORIGIN      
        1 gcagttgccc gggacgggca cgacctcgcg gtccgggagc atcttcgcga tctgctcgag
       61 cgcctgctcg tcctccggtc gttcggcgat cggcatcacg aggccgccgt tgttcgcgag
      121 gtagaagttg aggtacggga cgcgccccgg cggacgggtc tcgaacgcga tcacctcgaa
      181 ggctcgcccc ctcgcatcgc gggtgctctg cagccggtcg aggttggcgc gcccgcgttc
      241 gtggtccggg tcggccgggt cctcggggag caggagcgcc accgtggccg gcgcgaggta
      301 cggcgcgatc ccgtcgatgt ggccgtcggt gtcgcggtcg gtggagtgac cgaattccag
      361 ccagacgatc gcctcgaccc ccaggtagtc gcgcagtccc cgctcgatct gctcgcgcga
      421 gagcgtcggg ttgcggttgg gattcagcag gcattgctcg gtcgtgacga gggtgccctc
      481 gccgtcgacg aagaacgagc cgccctcgag cacgaacggg gcctcgtagc ggcgcatccc
      541 gaggtgctcg gcgatgaggc gcgggacctg cgcgtcgcgg tcgtacggca ggtacttctc
      601 gccccacgag ttgaacccga agtggacgag cgcgatccgg cccgcagcgt cccggacgaa
      661 gatcggcccg ttgtcgcgca tccacgagtc gtcgatcggt agcgggagca cctcgacgcc
      721 gtcgccgcag cgccggcgga cctcggtctg ttgctcggga tcgcagacca tgacgacggg
      781 ctcgaacgcc gcgatcgcgt tcgccacggt cgcgtagtcg cgctcggcct cctcgaaccg
      841 gtccccccag agctccttgc gcgtccgcgt gggccactcc atcaggcagg cttcgtgcgg
      901 ctcccactcc gcgggcatcg agaagccgtc ctcgcgtggg gtggactggt gttcgctcat
      961 cgcccctccc cccttggcgt atatgaaggc gtccaggggg aaggtacacg catccctcgg
     1021 acgggagcct cgtcgggctg cacgcgcgga ccgatctgct gtcgatcgtt gtcggtggct
     1081 cgggctagcg tccggccgat ggccaagctc gaaggaatcc gttcgctcga tctcattctc
     1141 gaggaggtga gatctgagcg agatctgcag ctctcgcact tcgatgcaat cgacaccaag
     1201 gcggggatcg tcctcgggtt cgcgggcgct ctcgtggcgc tggcgccgcg aggggcggaa
     1261 ctcctcctgg accttggccg tgctctcgcg gtcgcctgcg ggttcacctg cttgtcggcg
     1321 ttctggccca ggcgctactg gagcacgaac ctccggccgt tccgagacaa gtacctcgcc
     1381 gccgagctgg agttcacgaa gatccatctc ctcgacacac agatcggcat ggcggacagg
     1441 atgtccggca tcctcgcgtc caaggcgatg aggctcaagc tcgcgatgat cgcgcttgcg
     1501 ctcgcggtct tcctgacggc ggtcggtgtc gtcttagact gaaccaagga ggcgggatgg
     1561 agaaggaacc gaacgcggac cacggcggag aggcgacgaa gccggaagag acgccgcccc
     1621 cgttcgagcc ggatccagac atcgtcacct tcctcgaacg aggccggcga caggacccga
     1681 aacggacctg ggaagcgacc gagccagccg aacgctgatc cgcatcgcga acttcctcga
     1741 ccaccaggca ttgagccggt cggccaccgg ccgaggtacc ctgacgtcga cgagcgcatg
     1801 caaatcgacg ggttcacgtt cggctcgatc cggatcgacg gagtggacta cgagcacgac
     1861 gtcgtgatcg ctcgcgggcg ggtccgtagg cggaagaaga aggcgtcgaa gccgttccgc
     1921 g
//
LOCUS       DATBRK010000106         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1747643, whole genome shotgun sequence.
ACCESSION   DATBRK010000106 DATBRK010000000
VERSION     DATBRK010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747643"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VIH70_03250"
     CDS             <1..1124
                     /locus_tag="VIH70_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115409.1"
                     /translation="VQQGVETMGHLAGSADSSMQAMSAHDGTERNVRPVDQQISELAE
                     QLRLLYDRTGIAMSSLHESVAFLGDRMGVETRERLEEMNRAFEARVIGLANLVRSDSE
                     ALRRELVRTAEGHDETIARLLDERLHQVTETLGDSVAKTVQVKLDDSLSALTARTDEQ
                     TRLIDARAGETVSAIDRNLIRMVDALDDHFERLGADVGERAATAAELAIGARFDDVLA
                     RLHAAASVMEREGSQTRDALEATRSRLDETSGALDTRFVALAKLVRSDSETLSGQILA
                     DQDVTKQALRAMKELQASLPSEVIQMVEERFGSLAESIERSNEMLAARIDRMADTIGQ
                     RQNDDIQVVIDRMGDAMHALASLGKDPRSGGPGSGVDID"
     gene            complement(1145..1567)
                     /locus_tag="VIH70_03255"
     CDS             complement(1145..1567)
                     /locus_tag="VIH70_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY8115410.1"
                     /translation="MSVRFDDLAVGQDLPSSSRTVTRNDIEAYADASGDQNPLHRDDE
                     VARAAGFPRVVAHGMYTMGILTSWLVDWLGDPAGLLRLQAHFRAPVFVDESIECGGRV
                     RSLDPQARTAVLDVWVRVERGGAIEWPIRRSEAQVRPA"
     gene            complement(1564..>1852)
                     /locus_tag="VIH70_03260"
     CDS             complement(1564..>1852)
                     /locus_tag="VIH70_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8115411.1"
                     /translation="VPPTIVTVPELEAGLDHVVSDPRLGIDLSRILHGEQEYRWSRPV
                     AVGETLTASATIDSVRDKGGMRFLSLRTEVVDEAGELVASGRSTLIVRGDR"
BASE COUNT          311 a          639 c          652 g          250 t
ORIGIN      
        1 acgtccagca gggtgtcgag accatgggcc atctggccgg gagcgccgac tcgagcatgc
       61 aggcgatgtc ggcacacgac gggacggaga ggaacgtccg gccggtcgac cagcagatct
      121 ccgagctcgc cgagcagctg cggctcctct acgaccgaac cgggatcgcg atgagctcgc
      181 tgcacgagtc cgtggccttc ctcggcgacc ggatgggggt cgagacgcgc gagcggctcg
      241 aggagatgaa ccgggcgttc gaggctcggg tgatcgggct cgcgaacctc gtccgttccg
      301 actccgaggc gctccgtcgc gagctcgtga ggacggccga gggccacgac gagacgatcg
      361 cccggctgct cgacgagcgg ctgcaccagg tcaccgagac gctcggcgac tccgtcgcga
      421 agacggtgca ggtcaagctc gacgactcgc tgtccgcgct gaccgcacgc accgacgaac
      481 agacccggct gatcgacgcg cgggcgggcg agaccgtttc ggccatcgac cggaacctca
      541 tccggatggt cgacgcactc gacgatcatt tcgagaggct cggggccgac gtgggcgagc
      601 gggcggccac cgccgccgag ctcgcgatcg gcgcgcggtt cgacgacgtg ctcgcgcgct
      661 tgcacgcggc cgcctccgtg atggaacgcg agggttcgca gacccgggat gccctggagg
      721 ccacgcggag ccgcctggac gagacgagcg gcgccctcga cacgcgattc gtcgcgctgg
      781 ccaagctcgt acgttcggac agcgagacgc tctcggggca gatcctggcg gaccaggacg
      841 tcacgaagca ggcgctccgg gcgatgaagg agctacaggc gagcctgccg tccgaggtca
      901 tccagatggt cgaggagcgg ttcggctccc tcgccgagtc gatcgagcgt tccaacgaga
      961 tgctcgcggc tcggatcgat cggatggcgg acacgatcgg tcagcgccag aacgacgaca
     1021 tccaggtcgt gatcgaccga atgggcgacg cgatgcacgc gctcgcgagc ctcgggaagg
     1081 atccgcggtc gggtggcccc gggtccgggg tcgacatcga ctgacccctt gggggccacc
     1141 gcgatcaggc cgggcgaacc tgggcctcgc tcctacggat cggccactcg atcgcaccgc
     1201 cccgctcgac ccggacccag acgtcaagca cggccgtccg ggcctgcgga tcgagcgacc
     1261 tcacccgacc gccacactcg atcgattcgt cgacgaacac cggagctcga aagtgcgcct
     1321 ggaggcggag cagccccgcg gggtcgccca gccaatcgac gagccaggat gtcaggatgc
     1381 ccatggtgta catgccgtgg gcgaccacgc gtgggaagcc ggcggcgcga gcgacctcgt
     1441 cgtctcggtg gagcgggttc tgatcgcccg acgcgtccgc gtacgcctcg atatcgttgc
     1501 gtgtgaccgt gcgcgacgac gacggcaggt cctgccccac cgccagatcg tcaaatcgaa
     1561 cgctcaccgg tcgccccgga cgatcagcgt gctccgcccc gatgcgacga gctcgccggc
     1621 ctcgtccacg acctcggtcc gaaggctcag aaaacgcatg ccgcccttgt cgcggacgct
     1681 gtcgatcgtc gccgaggcgg tcagcgtctc gcctaccgcg acgggccgcg accaccggta
     1741 ctcctgttct ccgtggagga tgcgggaaag gtcgatcccg agccgcggat cggacaccac
     1801 gtgatcgagc ccggcctcga gctcgggcac ggtcacgatc gtcgggggca cg
//
LOCUS       DATBRK010000107         4126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_159357, whole genome shotgun sequence.
ACCESSION   DATBRK010000107 DATBRK010000000
VERSION     DATBRK010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159357"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VIH70_03265"
     CDS             complement(<1..1233)
                     /locus_tag="VIH70_03265"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEY8115412.1"
                     /translation="MTGLTARATRLGPAFDLLAGYREGGFFLERSGLGLATSGEVDRV
                     AAEPGPERILRLSRSILEALASIEAEPASPAAAAVASIPFDDREPAEAVLPRRATIRL
                     DAGETWQVEVAPRGETPHAGERERWTGRTLPHDAFEEMQLRPDPEPDEYVTAVGRATE
                     RIRSGELRKVVLARTMLVNADRTLDPKQLLWRLRAVDADCYAFAAPQPNAVTGSVLVG
                     ATPELLLRRRGRLVEATPLAGSSQRFGDAGRDRASADRLFRSGKDREEHAVVVQDVHH
                     ILDAFCEDLVHPHEPELLGTANVWHLATPFRGRLHDPSVTALELVAALHPTAAVCGSP
                     RETAREALEDLEPIERGGYAGPVGWMDANGDGEWAIALRCAEITGSMARLFAGAGIVA
                     DSEPEAELDETERKFRALL"
     gene            complement(1265..1966)
                     /locus_tag="VIH70_03270"
     CDS             complement(1265..1966)
                     /locus_tag="VIH70_03270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEY8115413.1"
                     /translation="MSGHVTDGGLPDPSEKAQVVRRMFDRISNRYDLVNRVMTFGMDV
                     GWRRRAVGELRLPGGALVADLACGTGDLCNELLAGGYRAIGFDFSHGMLRNARTEAPT
                     IEADVLRLPLADGRVDGATCGFALRNVTDLAELFVETARVLRPGGRAAFLETSEPDGR
                     VLRAGHALYFRRIVPLIGGALSDGEAYRYLPRSTAYLPEPHRFLEMLGDAGFERVERT
                     PLGGGVAQMLVGTRG"
     gene            complement(1963..2658)
                     /locus_tag="VIH70_03275"
     CDS             complement(1963..2658)
                     /locus_tag="VIH70_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8115414.1"
                     /translation="MRYRVEQLAAACDVSVDTIRYYQTRGLVPPPAREGRVAWYDDRH
                     AERIREVREFRRRGLTLAAIERVLGDGLDPADADLAVAIAAARGEARQDELLTLDGFA
                     SRSGVPASLIQAVEREGIELGRTVDGEVRYTAADIDMVRGALRLLEYGLPLGELLSLA
                     RDTNEAMVALAARAVELFDEHVRKPIRDTAGAEDAAAQLVEAFDTLLPAVTNLVANHF
                     RRVLLAEAEERLE"
     gene            complement(2708..3496)
                     /locus_tag="VIH70_03280"
     CDS             complement(2708..3496)
                     /locus_tag="VIH70_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY8115415.1"
                     /translation="MSHPEDLLADYIDGALEERERAAVDAHLLGCARCSSEVRQAGAA
                     RAALSELENVPVPFGVTGPVLAEAGRRFERRPGVVWGRFQWAAGLAAAAALVVVVALN
                     VGGGDARNAAAPERTTAVAGGTGAGGAEAATPGVTFAGLERQTDVNYDEAGIQAVASQ
                     AAGAIAEAGDARSSQAALATSAEDSKERTSLARDCVDRSGVRGPNDVLIRLIEARFEG
                     TPAFIGVFSEGPGAGQAPDHIVVFVVAKDDCRILSTASQRIPPG"
     gene            complement(3493..>4126)
                     /locus_tag="VIH70_03285"
     CDS             complement(3493..>4126)
                     /locus_tag="VIH70_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8115416.1"
                     /translation="SGDRAAFAALVERHERRVYNLALRMTGREEDARDATQDAFLTVL
                     RKLSSFRGEAAFTTWLHRVTVNACYDLLRKRQRAPLLDRGEDDLPALEPPPAPDHADA
                     SSLSMDVQQALLQVPEDFRAVMILHDVHDLRQEEVAAIVGVPIGTVKSRLHRGRIALA
                     RAMGIEQRERASGDPEGPPALGGSPEAGPAAPRSGERAEAAGPSEGTVSP"
BASE COUNT          532 a         1557 c         1492 g          545 t
ORIGIN      
        1 gagcagcgcc cggaacttgc gctcggtctc gtcgagctcg gcctcgggtt ccgagtcggc
       61 gacgatgccg gcgccggcga acagccgggc catcgagccc gtgatctccg cgcaccgcag
      121 cgcgatcgcc cattcgccgt cgccgttcgc gtccatccag ccgaccggcc cggcgtaccc
      181 gccccgctcg atcggctcga ggtcctcgag cgcctcgcgg gccgtctcgc gcggcgaccc
      241 gcacacggcg gctgtggggt ggagcgcggc cacgagctcg agcgccgtga ccgacggatc
      301 gtgcaggcgc ccccggaacg gcgtcgcgag gtgccacacg ttcgcggtgc cgagcagctc
      361 cggctcgtgc gggtggacga ggtcctcgca gaacgcatcg aggatgtggt ggacgtcctg
      421 gacgaccacc gcgtgctcct cgcgatcctt gcccgagcgg aacagccggt ccgccgaagc
      481 tcggtcgcgc ccggcgtcgc cgaaacgctg cgacgacccc gcgagcggcg tcgcctcgac
      541 gagccggccg cggcgacgga gcaggagctc gggcgtcgcg ccgacgagca ccgaacccgt
      601 caccgcgttt ggctgcgggg cggcgaacgc gtagcagtcc gcgtcgaccg cgcgcagtcg
      661 ccacaacagc tgcttggggt cgagcgttcg atccgcgttg acgagcatcg ttcgcgcgag
      721 cacgaccttg cggagctcac ccgagcggat ccgttcggtc gcccggccaa ccgccgtcac
      781 gtactcgtcg ggctccgggt ccggccggag ctgcatctcc tcgaacgcgt cgtgcgggag
      841 cgtccggccc gtccatcgtt cgcgctcgcc cgcatgcggg gtctcgccgc gcggggcgac
      901 ctcgacctgc caggtctcgc cggcgtccag gcggatcgtc gcccgtcgcg ggaggaccgc
      961 ctccgcgggc tcacggtcgt cgaacgggat cgacgccacc gccgccgcgg cgggcgaggc
     1021 gggttcggcc tcgatcgagg cgagtgcctc gaggatcgaa cgcgatagtc gcaggatgcg
     1081 ttcgggtccc ggctcggccg cgacccggtc gacctccccg ctcgtcgcga gcccgagccc
     1141 ggagcgctcg aggaagaagc cgccctcccg gtacccggcg agcaggtcga acgccggacc
     1201 gagccgggtc gcccgagctg tgagaccggt cacgtcgccg acctcgacgg tcgcgctggc
     1261 gcggtcatcc gcgcgtcccc acgagcatct gggccacgcc gcccccgagc ggcgtcctct
     1321 cgacccgctc gaaccccgcg tcgccgagca tctcgaggaa ccggtggggc tcggggaggt
     1381 aggccgtcga cctggggagg tatcggtagg cctccccgtc cgagagcgcc ccgccgatca
     1441 gcgggacgat ccgccggaag tacagcgcgt gtcctgcccg gaggacgcgt ccgtcgggct
     1501 cgctcgtctc gaggaacgcg gcgcgcccgc ccggccggag cacgcgcgcg gtctcgacga
     1561 acagctcggc caggtcggtc acgttgcgca gcgcgaaccc gcacgtggcg ccgtccacgc
     1621 ggccgtccgc gagcggaagc cgcaggacgt cggcctcgat ggtcggcgcc tcggtgcggg
     1681 cgttgcggag catgccgtgc gagaagtcga agccgatcgc ccggtacccg ccggccagca
     1741 gctcgttgca caggtcgccc gtgccgcacg cgaggtcggc cacgagggcg ccgccgggca
     1801 ggcggagctc gccgaccgcg cgacgtcgcc acccgacgtc catcccgaag gtcatgacgc
     1861 ggttcacgag gtcgtagcgg ttcgagatcc ggtcgaacat gcggcggacc acctgggcct
     1921 tctcgctcgg gtccgggagg ccgccgtcgg tcacgtggcc gctcactcga gccgctcctc
     1981 ggcctccgcg agcaggaccc ggcggaagtg gttggcgacc aggttcgtca cggcgggcag
     2041 cagcgtgtcg aacgcctcca cgagctgcgc cgcggcgtcc tcggcgccgg ccgtgtcacg
     2101 gatcggcttg cgcacgtgct cgtcgaacag ctcgaccgcc cgggcggcga gcgcgaccat
     2161 ggcctcgttc gtgtcccgcg cgagcgacag gagctcgccg agcggcagcc cgtattcgag
     2221 gaggcggagc gcgccgcgga ccatgtcgat gtcggcggcc gtgtaccgga cctcgccgtc
     2281 gaccgtgcgg ccgagttcga tcccctcgcg ctcgaccgcc tggatcaggg aggcggggac
     2341 gccgctgcgg ctggcgaagc cgtccagggt gaggagctcg tcctgacgag cctcgccgcg
     2401 ggccgccgcg atcgcgacgg ccaggtcggc gtcggcgggg tccagcccat cgccgaggac
     2461 acgctcgatc gccgcgagcg tgaggccccg gcggcggaac tcgcggacct cgcggatccg
     2521 ctcggcgtgg cgatcgtcgt accacgcgac gcggccctcg cgggcgggcg gcgggacgag
     2581 gccgcgggtc tggtagtagc ggatcgtgtc gaccgagacg tcgcacgcgg ccgcgagctg
     2641 ctccacgcga tacctcacga ccggtaactc tacgagtgaa cgctcgaacc ttcacccgcc
     2701 gcggagctca gccgggcggg atcctctggg aggcggtgct caggatgcgg caatcgtcct
     2761 tcgcgaccac gaacacgacg atgtggtcgg gggcctgccc ggcgccgggc ccctccgaga
     2821 acacgccgat gaacgccggc gtgccctcga accgcgcctc gatcagccgg atcaacacgt
     2881 cgttcggacc gcggacaccg ctccggtcga cgcaatcccg cgcgaggctc gtgcgctcct
     2941 tcgagtcctc ggcgctggtc gccaacgccg cctggctgct ccgcgcgtcc ccggcctcgg
     3001 cgatcgcgcc ggccgcttgg ctcgccaccg cctggatccc cgcctcgtcg tagttcacgt
     3061 cggtctggcg ttcgagaccc gcgaaggtga ctccgggcgt cgccgcttcg gcgccgcccg
     3121 ccccggttcc accggcgacc gccgtggtcc gctccggggc cgcggcgttc cgggcgtccc
     3181 cgccgccgac gttgagggcc accaccacga cgagcgcggc ggccgcggcc agtccggcgg
     3241 cccactggaa gcgaccccat accacgcccg gacggcgctc gaaccgccgc ccggcctccg
     3301 ccagcacggg gccggtcacg ccgaacggca cggggacgtt ctcgagctca ctcagcgcgg
     3361 ccctggcggc ccccgcctgc cggacctccg agctgcaacg cgcgcagccg agcaggtgtg
     3421 cgtcgacggc ggcgcgctcg cgttcttcca gcgcgccgtc gatgtagtcg gcgaggagat
     3481 cttcgggatg gctcacgggg atactgtccc ttctgacggc ccggcggcct ccgcccgttc
     3541 ccccgatctc ggagccgcgg ggccggcttc gggggacccg cccaacgcag gtggaccttc
     3601 cgggtccccg gaggcacgct cccgctgctc gatccccatc gcccgtgcca gcgcgatccg
     3661 gccgcgatgc aggcgcgatt tcacggtccc gatggggacg cccacgatcg cggccacctc
     3721 ctcctgccgg aggtcgtgga cgtcgtgcag gatcatcacc gcgcggaagt cctcgggcac
     3781 ctgcaggagg gcctgctgca cgtccatcga caggctcgag gcatcggcgt ggtcgggcgc
     3841 cggcgggggc tcgagcgcgg ggagatcgtc ctcgccgcga tcgagcaacg gcgctcgctg
     3901 gcgcttgcgc aacaggtcgt agcaggcgtt gaccgtcacg cggtggagcc atgtcgtgaa
     3961 cgccgcctcg ccgcggaacg acgaaagctt gcggagcacc gtgaggaagg cgtcctgcgt
     4021 ggcgtcgcgc gcgtcctcct cccgtcccgt catgcgcagg gccaggttgt agacccgccg
     4081 ctcgtggcgt tcgaccagcg cggcgaaggc ggctcgatct cccgac
//
LOCUS       DATBRK010000108         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1890362, whole genome shotgun sequence.
ACCESSION   DATBRK010000108 DATBRK010000000
VERSION     DATBRK010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890362"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1558)
                     /locus_tag="VIH70_03290"
     CDS             complement(74..1558)
                     /locus_tag="VIH70_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8115417.1"
                     /translation="MILWRRGAPDRRRREELEAWEEVRVTSTAGWRALDRAISGDVIL
                     PDSREFDIGYPALNARFDDVRPQAVVRCRSSEDVAETIRFAHGAGMEVATRNGGHCFG
                     GRSSTKGLLVDVTPLRSVEISAGTVTVGGGTRLGEVYGSLLEQDLAIPGGSCPSVGIA
                     GLALGGGLGIIGRKHGLTSDHLVRAQIVLADGRALECEEHREEDLFWALRGAGAGNFG
                     VVTSLTFRTIAAPAATNFHLAWPFSKAAAVIDAWQSWAPTAPDELAASLLIGASAEPE
                     DPPSVGVFGVMLGTEPDTAELVDELVTRMGSDPDSAFRKHMSFRQTTRYWGERAARER
                     VEGEPPGWELRGHRFIKSEFFQRPISAEAIGALLENFRNGRAREQTRELDFSPWGSGY
                     NRMSADATAFVHRDALFWLKHATEVQPSASTAEKDAAHDWVTRSWATVHPWGTGRVFP
                     NFPDPDLQDWASAYFGTNLDRLLRVKARYDPGNLFRFRQSLPVH"
BASE COUNT          296 a          605 c          543 g          276 t
ORIGIN      
        1 cttcgccgac ctcggcatca ccgccagcgc cgcggcatag acaacagttt gtcggtccgc
       61 gttccgcaag cgcctagtgc acgggcagtg actgacggaa ccggaagagg ttccctggat
      121 cgtatcgggc cttgaccctc agcaggcgat ccaggttcgt gccgaaatag gcgctggccc
      181 aatcctgtag atccggatcg gggaagttgg ggaacacacg tcccgtgccc cagggatgca
      241 ccgtggccca tgatcgagta acccagtcgt gggctgcatc cttctcagcc gtagacgcgc
      301 tgggttggac ctcggtggcg tgcttcagcc aaaagagggc gtctcgatgt acgaaggcgg
      361 tggcatcggc gctcatgcgg ttgtagccgc tgccccacgg cgagaaatcc agctcgcgag
      421 tctgctcacg cgctcggccg ttccggaagt tctcgagcaa cgcgccgatc gcctccgctg
      481 agataggccg ctggaagaac tccgacttga tgaagcggtg accgcgtagc tcccagcccg
      541 gaggctcccc ctccacccgc tctcgggccg cgcgctcccc ccagtacctc gtcgtctgcc
      601 ggaaggacat gtgcttgcgg aaggccgagt ctggatccga gcccattcgg gtcacgagct
      661 cgtcgacgag ctccgccgtg tcgggctcgg tgcccagcat caccccgaac acgcccacag
      721 aaggcgggtc ctcgggctcg gcgctcgccc cgatcagcag actggcggcg agctcgtcgg
      781 gggcggtagg ggcccaggac tgccacgcat cgatcaccgc ggcggccttg gagaagggcc
      841 aggccaggtg aaagttcgtc gctgccggtg cggcgatggt gcggaacgtg agagaagtca
      901 ccaccccaaa gttcccggct ccggcgccgc ggagggccca gaacaggtct tcctcgcggt
      961 gctcctcgca ctccagagcc cgaccgtccg ccaggacgat ctgtgcgcgg accagatgat
     1021 ccgaggtgag cccgtgcttt cggccgatga tccccaggcc cccaccgaga gcgagaccgg
     1081 cgatcccgac cgacggacag gagcccccag gaatagcgag gtcctgttcc agcagcgatc
     1141 cgtacacctc gcccaggcgg gtgcctcccc ccaccgtcac cgtgcccgcg gagatctcga
     1201 cggaacgcag gggggtgacg tcaacgagca gtcctttcgt cgaggacctt ccgccgaagc
     1261 agtgaccgcc gtttctcgtt gctacctcca tcccagcccc gtgggcgaac cggatagtct
     1321 cagcaacgtc ctccgagctt cggcaccgga cgaccgcctg aggccgcaca tcgtcgaacc
     1381 gggcgttgag cgccgggtac ccgatatcaa actcacgaga gtcgggaagg atgacgtctc
     1441 cgctgatcgc tctatccagc gctctccaac cggctgttga ggtcacgcgc acctcctccc
     1501 acgcttccag ttcctcccgg cgccgtcgat cgggagctcc tcgtcgccac aagatcatgg
     1561 cggtgcccgc gaggtacgcc tagtcagcat ggaacggccg gctccggtcc gcatctgcgt
     1621 gggtcaccca cactgagcgg gcggagccac catacggaag gagccggccg tggccagtgt
     1681 cgcccatctt gccccgatcc atctcggcct ggacgtccac
//
LOCUS       DATBRK010000109         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1603554, whole genome shotgun sequence.
ACCESSION   DATBRK010000109 DATBRK010000000
VERSION     DATBRK010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603554"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1502
                     /gene="carB"
                     /locus_tag="VIH70_03295"
     CDS             <1..>1502
                     /gene="carB"
                     /locus_tag="VIH70_03295"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710836.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEY8115418.1"
                     /translation="LLPTLGGQTALNLALELVAAGVLEALDIELIGAGPDVIRRAEDR
                     ELFRETVRLAGLKVPESTIVTDPADLPIDLSFPVILRPAFTLGGHGGGTARSWSELEG
                     LLERALAESPVRQVLVEESLLGWDEFELEVIRDRRDNVVIVCSIENLDPMGVHTGDSV
                     TVAPQMTLSDEAYQELRDAAADVIRAVGVECGGSNIQFARHRESGELRVIEMNPRVSR
                     SSALASKATGYPIAKVAAKLAVGYTLDEIPNDLTGTTPASFEPTLDYVVVKAPRFAFE
                     KFPGAETELGTQMKSVGEAMGIGRTFTEAFLKAIRSRELDGGAVTPWEALDAVPSGVH
                     PWFRRELESVVAALARVHSLDDLVADDWLRLKRLGLSDADIAAQSGTTEEQARAKRRS
                     WGVKPAFRRVDSCAGEVEAGSNYLYSTWGEQDEAPPAPGRSVIILGSGPNRIGQGIEF
                     DYCCVHAAKTFRELGYEAVMVNCNPETVSTDYDTSNRLYFEPLCTEEVLA"
BASE COUNT          235 a          512 c          525 g          230 t
ORIGIN      
        1 ctgctgccga cgctcggcgg tcagaccgcg ctgaacctgg cgctggagct cgtcgccgca
       61 ggggtgctcg aggcgctcga tatcgagctg atcggcgccg gccccgacgt gatccgccgc
      121 gccgaggacc gcgagctctt ccgcgagacc gtccggctcg ccgggctgaa agtgccggag
      181 tcgacgatcg tcaccgatcc cgccgaccta ccgatcgact tgagcttccc cgtgatcctg
      241 aggcccgcgt tcaccctcgg cggccacggc ggcggcaccg caaggtcctg gtccgagctg
      301 gaaggactcc tcgagcgggc cctcgccgag agtccggtgc ggcaagtgct ggtcgaggag
      361 tccctgctgg gctgggacga gttcgagctc gaggtgatcc gcgaccgccg cgacaacgtc
      421 gtcatcgtct gctcaatcga gaacctcgac ccgatgggcg ttcacacggg cgattcggtc
      481 acggtcgcgc cgcagatgac gctctcagac gaggcgtacc aggagctgcg ggacgcggcc
      541 gcggacgtca tccgcgccgt cggcgtcgaa tgcggcggct cgaacatcca gttcgcccgc
      601 catcgggaga gcggcgagct gcgagtgatc gagatgaacc cgcgcgtctc gcgctcgtcg
      661 gcgctcgcct cgaaggcgac gggctacccg atcgccaagg tcgccgccaa gctggcggtc
      721 ggctacacgc tcgacgagat cccgaacgac ctgaccggca cgaccccggc cagcttcgag
      781 ccgaccctcg actacgtcgt cgtcaaggcg ccgcgctttg cgttcgagaa gttcccgggc
      841 gcggagaccg agctcggcac acagatgaag tcggtcggcg aggcgatggg gatcggccgc
      901 acgtttacgg aggcgttctt gaaggcgatc cgctcgcggg agctcgacgg cggcgcggtc
      961 acgccctggg aggcgctcga cgcagtcccg tcgggtgtcc atccctggtt ccggcgggag
     1021 ctggagagcg tggtcgcggc cctcgcgcgc gtgcactccc tcgacgatct cgtcgcagac
     1081 gactggctac ggctgaagcg gctcgggctc tcggacgccg acatcgccgc ccagtccgga
     1141 acgacggagg aacaggcgag agccaagcgc cgttcctggg gcgtcaagcc ggcctttcgc
     1201 cgggtcgatt cctgcgccgg tgaggtcgag gcggggtcga actacctcta ctcgacctgg
     1261 ggggagcaag acgaggcgcc gcctgcgccc ggccgctctg tgatcatctt gggttcaggc
     1321 ccgaaccgga tcggccaggg gatcgagttc gactactgct gcgttcacgc ggcgaagacg
     1381 ttccgcgagc tgggctacga agccgtgatg gtgaactgca accccgagac cgtctcgacc
     1441 gactacgaca cctccaaccg gctctatttc gagccgctgt gcaccgagga ggtgctggcg
     1501 at
//
LOCUS       DATBRK010000110         8681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_159874, whole genome shotgun sequence.
ACCESSION   DATBRK010000110 DATBRK010000000
VERSION     DATBRK010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159874"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VIH70_03300"
     CDS             <1..1246
                     /locus_tag="VIH70_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8115419.1"
                     /translation="QHFVLVDPFTVTENIILGEEGGERLDMDAAQQRVSDLAETYAFR
                     VDPRAVVEDLSVGEEQRVEILKALYRGVEILILDEPTAVLTPNETKDLFGNLRKLREA
                     GRTIVFISHKLDEVMEIADRITVLRRGRVVGETRPAETTKAKLAEMMVGRPVLFRLEK
                     PSVQIGEPVLRVGDLTGEGKLNGVSLEVRAGEILGVAGVEGNGQRELAETLIGLRAPE
                     SGTIELEGSVISGRAVFDIRNAGVGYIPEDRHEQGLVLTMSLWENSVLGRQDDEEFAG
                     RLGVLFIRKIKDLAARLVELFDIRTRSVEVTASTLSGGNQQKLILARELETDPKLLIA
                     AQPTRGLDVGAIEFVWKEILEQKAAGRGVLLISAELDEIYALSDRIVTLYEGKITGEF
                     PPDAPPEQIGIGMLGRAPEAAA"
     gene            1246..2487
                     /locus_tag="VIH70_03305"
     CDS             1246..2487
                     /locus_tag="VIH70_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8115420.1"
                     /translation="MAAEGMAAPVAAPRARPAWLENFVGQFLALLASFGIALLLGSLL
                     IVAYGENPIDVYRSIFDASLGSTDGIGYVLAIATPLIFSALAVSVCFKGGLFNIGVEG
                     QYLVAMITAAVAELNLDFLPGFLLVPAVLLAAMLGGMIWAAIPGVLKVKRGAHEVVTT
                     IMLNAIALSLVGWALNGPLHYDDAPEGAFVDLRTNVFPAKGLVGDLGSAFGVTPGAHL
                     SWLLFLAIAAAIVVWFLIRRTRLGYEARAVGSSPGSARAGGVSIGSTQIRLFLISGAL
                     AGLIGMQQILADHGYLPLNYVVGLGFLGIGVAFLGQNNPIGIVFAAILWGTLSRGEVA
                     LQISSQVPREFIIILQGLLILTVVITYQIAKRQLAARQLQRAGAAGELEDSAAPEESS
                     GPRGPEETGAEALAPADEKRI"
     gene            2487..3461
                     /locus_tag="VIH70_03310"
     CDS             2487..3461
                     /locus_tag="VIH70_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8115421.1"
                     /translation="MPFTDFAVAIQISFTYFTVFYLTGLGGLFSERSGIVNIGLEGLM
                     IVGTVTGAFGVRFFTDTVGLGTVWGPILGLLFGALCGAIFAGVHAVATITFRVDHIVS
                     GVVINVVAIGLARFLSSAFFNGQSTQSDPGGPHLARLDIPGLSALPGGLDRAFEGLSP
                     MVIVAALMVIPVWYSLNRLRWGLRLRSCGENPEATRSLGVSVPLYRYQGVLLSGAFAG
                     LSGAFLAAEIIGNWHEGQTLGLGFIALAALIISNWNPIRLMFFAWLFGFAFAIPLRLN
                     DAPVISLLPEQFIRMIPYVVTIVAIAGFVGRVRPPAAAGRAYEGGGGG"
     gene            complement(3468..4085)
                     /gene="upp"
                     /locus_tag="VIH70_03315"
     CDS             complement(3468..4085)
                     /gene="upp"
                     /locus_tag="VIH70_03315"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY8115422.1"
                     /translation="MSVTVVDHALAGHLLAQLRSQETAPPVFRTLAKRLALTLALEAI
                     RDLPTTEIAVRTPLEETTGRVLGDLVAVPILRAGLGMLEAVTELFPEVAVGYIGLERD
                     EASLEPQSYYRKLPPVKGRHVLVLDPMLATGGSGSAATSAVREGGPRSIRFVCVVAAP
                     EGIKRMEADHPDVPIFTAAIDRQLNERGYIVPGLGDFGDRLFGTE"
     gene            4344..5369
                     /gene="aroF"
                     /locus_tag="VIH70_03320"
     CDS             4344..5369
                     /gene="aroF"
                     /locus_tag="VIH70_03320"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY8115423.1"
                     /translation="MVVVMRREATEADIELVSERVRAAGGEAFVSRGAVHTIVGLVGD
                     TERFQAIPWGQLPGVDHVIQVGKPYKMVAADLHPTPTTVNVGSTPVGRGTFTLIAGPC
                     AVESEEQAMLATKAARAAGATILRGDVYKPRTSPYSFQGLGERGLEVLEECRRQTGLP
                     IVVEVVDVSQVEHVAAVADCIRVGTRNAQNFELLKEVGLSRRPVMLKRGLAMTIEEWL
                     QAAEYVAQRGNSEIILCERGIRTFEPATRNTLDLAGMAVAQTESHLPVIVDPSHAVGK
                     RHLVAPMSRAALAAGADAVMIDVHPDPESALCDGPQAVTPDEMLDLGRELERLAAALG
                     RPFNHAE"
     gene            5501..7909
                     /locus_tag="VIH70_03325"
     CDS             5501..7909
                     /locus_tag="VIH70_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115424.1"
                     /translation="MRMRIGRTFFALAIAAPLIAVVAPAAPTAVAQPACELGNGIEHV
                     VNIVFDNVHFFRDNPNVPSDIEQIDSLNDFIQDNGVMLSNNHTPLIAHTAEDSLAIYT
                     GLYGDRHGMPISNSYRTWNPNGTVNFGNGPFQGASSFLYWTDPNNDGGKDTKHAMIYS
                     DSVPASATTTNVATPAPWVPWTRAGCDVGDVSTANMVLENPGFDIPKVFGPNSPETAQ
                     LIADTPPPPAPAFFNQETADYVGLAVHCAQGSPFCADAEAIKFGQVTPSPTAVDDVLP
                     DEPGGYTGYQALFGHKYIAPQLGAGNVSLTHNGYPVTNAAGNLVDLSGGQINGAFLPP
                     NTPGFPGFGPIVAKQTLAYMADMLEADVPVVYGYIGDIHERKAGQSGCSTSTAVAAGW
                     ALGPGDNCYRQTAEQYDQAFQTFFDRLAAAGITKDNTLFVFGAEENDQFDGANVGRAI
                     EPTQGGTPGCLGFSADTYCYASGQIGEINTNLPGLLAAQKGNTTPFAIEPQGAVIYVN
                     GHPGPNDPAVRQLERDIGALTNPHDPYTGNDDESITAYMAGSVEQEILHLVNADPDRT
                     PTFTLFPKPDYFFGSPVTCTPADQAPCASNTLASAARFAWDHGYYAPTIDITWAGFVG
                     PGVANLRLDGPEADEGPALEDPDGGGTVPEFSRVGTWVDLPDIRPTILWLVGLQDSYV
                     NDGRVLSEILTDPNAAIAGSRYTDLAACYKQVNASVGEFGTNTLIADTAALKSGGGGD
                     GLFKSTQTRLSKLLSQRDALAPELNNRLFDAAFDDVALPAATNLLDRCGSLLEQAEEL
                     ASGE"
     gene            complement(7922..>8681)
                     /locus_tag="VIH70_03330"
     CDS             complement(7922..>8681)
                     /locus_tag="VIH70_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115425.1"
                     /translation="VSANVVDQRVWATSGEQGPPGVYLLGSPGTALPFVVTRAWKVGT
                     GMVTEEIHFYGPSGRLVHRWGPAVRRMIGSMDLTVETDTVTDASFDETGTYVVSFVID
                     EEIVGEIEVPVLVQLASAKLPKVIEDGLKRSDVIWVGVEANGRRKLIPSWFVYRNGRI
                     FVLSQRKPGPEDQSVPGIPGASELVVVTRRKGRDTAATEFHAASRVLQGPEWEEAAKL
                     LVDKRKSRAGTPSEWLARWRGSCDIAELTPVLPG"
BASE COUNT         1369 a         2963 c         2979 g         1370 t
ORIGIN      
        1 ccagcacttc gtcctggtcg atcccttcac ggtcaccgag aacatcatcc tgggggagga
       61 gggcggcgag cgcctcgaca tggacgcggc gcaacagcgt gtctccgatc tcgccgagac
      121 ctacgcgttc cgcgtcgacc cccgcgccgt cgtcgaggat ctgtccgtgg gcgaggaaca
      181 gcgcgtcgag atcctcaagg cgctgtaccg gggggtggag atcctcatcc tggacgagcc
      241 gaccgccgtc ctcaccccca atgagaccaa ggacctgttc ggcaacctcc gcaagctccg
      301 ggaagcgggg cggacgatcg tgttcatcag ccacaagctc gacgaggtca tggagatcgc
      361 ggaccggatt acggtgcttc gccggggacg cgtggtcggc gagacgcgac ccgccgagac
      421 caccaaggcg aagttggccg agatgatggt gggccggccc gtgctgttcc ggctcgagaa
      481 gccctcggtg cagatcggcg agccggtcct gcgggtcggg gacctcaccg gcgaggggaa
      541 gttgaacggc gtgagcctgg aggttcgtgc cggcgagatc ctcggagtcg ccggcgtgga
      601 ggggaacggt cagcgtgagc tggcagagac actgatcggc ttgcgggcgc cggagtcggg
      661 cacgatcgag ctcgagggat ccgtgatctc ggggcgcgcc gtgttcgaca tccgcaacgc
      721 cggcgtcggc tacatccccg aggaccggca cgagcagggt ctggtcctga ccatgtcgct
      781 gtgggagaac tcggtgctgg gtcggcagga cgatgaggag ttcgccggac ggctcggcgt
      841 cctgttcatc cggaagatca aggacctcgc cgcgcggctg gtggagctgt tcgacatccg
      901 aactcggagc gtcgaggtga cggcgtcaac gctctcgggt gggaaccagc agaagctcat
      961 cctcgcgcgg gagctggaga ccgatcccaa gctcctgatc gccgcgcaac ccacgcgcgg
     1021 gctggacgtc ggggcgatcg agttcgtctg gaaggagatc ctcgagcaga aggccgcggg
     1081 ccgcggcgtg ctgctgatct cggccgagct cgacgagatc tacgcgctgt ccgaccggat
     1141 cgtgacgctg tacgagggca agatcacagg cgagttccca cccgacgccc cgcccgagca
     1201 gatcgggatc ggcatgctcg gccgtgctcc cgaggcggcc gcgtagtggc cgccgaaggg
     1261 atggccgcac ccgtcgccgc accgcgcgcc cgtccggcgt ggttggagaa cttcgtcggc
     1321 cagttcctgg ccctgctcgc ctcgtttggg atcgccctgc tgctcggctc gctcctgatc
     1381 gtggcctacg gcgagaaccc gatcgacgtc taccggtcga tcttcgacgc gtcgctgggc
     1441 tcgaccgacg gcatcggcta cgtcctggcg atcgctacgc cgctgatctt ctccgccctc
     1501 gccgtgtccg tgtgcttcaa gggcggcctc ttcaacatcg gcgtcgaggg tcaatacctc
     1561 gtcgcgatga tcacggccgc ggtggccgag ctcaacctgg acttcctccc cggcttcctg
     1621 ttggtaccgg ccgtgctgct cgcggccatg ctcggcggca tgatctgggc cgcgatcccc
     1681 ggcgtcctga aggtcaagcg aggtgcgcac gaggtggtca cgacgatcat gctcaacgcg
     1741 atcgccctca gcctggtcgg gtgggccctc aacgggccgc tgcattacga cgacgcgccc
     1801 gagggggcct tcgtcgacct gcggacgaac gtgttccccg ccaagggtct ggtcggcgat
     1861 ctcggctcgg cgttcggcgt cacgccggga gcgcacttgt cgtggctgct gttcctggcg
     1921 atcgccgccg cgatcgtcgt ctggttcctg atccgccgga cgcgccttgg ctacgaggcg
     1981 cgggcggtcg ggtcatcgcc gggttcggcc agggcggggg gcgtgtcgat cggctcgaca
     2041 cagatccgct tgttcctgat ctcgggggcg ctcgccggcc tcatcgggat gcagcagatc
     2101 ctggcggacc acggctacct gcccctgaac tacgtcgttg gattgggctt cctcgggatc
     2161 ggggtcgcct tcctgggcca gaacaacccg atcggcatcg tcttcgcggc gatcctgtgg
     2221 gggaccctct cgcgaggaga ggtcgccctc cagatctcca gccaggtgcc gcgcgagttc
     2281 atcatcatcc tgcagggcct gctgatcctg accgtcgtga tcacgtacca gatcgccaag
     2341 cgccagctcg ccgcccgtca gctgcaacgg gcgggggcgg ctggggagct ggaggactcc
     2401 gcggcgccgg aggagagctc gggaccgcgc ggtcccgagg agaccggagc cgaggccctc
     2461 gcgccggcgg acgagaagag gatctgatgc cgttcacgga cttcgccgtc gcgatccaga
     2521 tctcgttcac gtacttcacg gtcttctacc tcacggggct cggcggactg ttcagcgagc
     2581 gctcgggcat cgtgaacatc ggcctggagg gcctgatgat cgtcgggacg gtgacgggcg
     2641 cgttcggcgt gcgcttcttc accgacacgg tggggctggg cacggtgtgg ggcccgatcc
     2701 tcggactctt gttcggcgcc ctgtgcgggg cgatcttcgc cggcgtgcac gcggtcgcga
     2761 cgatcacgtt ccgcgtcgac cacatcgtgt cgggcgtggt gatcaacgtg gtcgcgatcg
     2821 gcctcgcgag gttcctgtca tccgcgttct tcaacggcca gtcgacgcag tccgaccccg
     2881 gcggtccgca cctggcgcgg ctggacatcc ccggcctttc ggcgttaccg ggcggcctgg
     2941 accgggcctt cgagggcctg tcgccaatgg tgatcgtggc cgccctgatg gtgatccccg
     3001 tgtggtactc gctgaaccgc ctgcggtggg ggctccggct tcggtcctgc ggcgagaacc
     3061 ccgaggcaac ccggtcgctc ggcgtgagcg tcccactgta ccggtaccag ggggttctgc
     3121 tgtcgggcgc gttcgccggg ctgtcgggcg cattcctggc cgcggagatc atcggcaact
     3181 ggcacgaggg gcaaacgttg ggcctggggt tcatcgcgct cgccgcgctc atcatctcga
     3241 actggaaccc gatccggctg atgttcttcg cctggctgtt cgggttcgcg ttcgcgatcc
     3301 cgctgcggct gaacgacgcc ccggtgatct cgctgctgcc ggagcagttc atccggatga
     3361 tcccctatgt cgtcacgatc gtggcgatcg ctggcttcgt cggacgcgtg cgaccgcccg
     3421 cggccgccgg cagggcgtac gagggcggcg gcggaggcta gtcggtccta ctccgtcccg
     3481 aacaaccgat cgccgaagtc gccgagaccc ggaacgatgt agccgcgctc gttcagctgc
     3541 cggtcgatcg ccgcggtgaa gatcgggacg tccgggtggt cggcctccat ccgcttgatc
     3601 ccttcaggcg cggcgacgac gcagacgaag cggatcgacc tgggcccgcc ctccctcacg
     3661 gcggatgtcg ccgccgaacc cgaaccgccc gtggcgagca tcgggtcgag caccaggacg
     3721 tgccggccct tcaccggagg gagcttgcgg tagtacgact gcggctcgag cgacgcctcg
     3781 tcgcgttcca gaccgatgta gccgacggcg acctccggga acagctcggt caccgcctcc
     3841 agcatcccga gccccgcgcg gaggatcggc accgcgacga gatcgccgag cacgcgtccg
     3901 gtcgtctcct cgagcggggt ccgcacggcg atctccgtcg tcggcagatc acggatcgct
     3961 tcgagcgcga gcgtcagggc caggcgcttg gcgagcgttc tgaacaccgg cggcgccgtc
     4021 tcctgcgagc ggagctgtgc caggagatgg cctgcgagcg cgtgatccac cacggtcacg
     4081 gacacgcgcg catcctaccg ggacgcgcga ggccccgctc acgcgggacg cgtccgtcga
     4141 tcgatgggtt gccagaccac gacggggctg ccacgatggc cccctgatgc gcgagacctt
     4201 cggttcgtgg ttctacggcg ggcggtgccc ggcctagtcg cctgaaccga aggaacaggg
     4261 accccgggcc gggagcccgg ggtttcgtcg tttccgaggc cctatcgcgc gccgagggga
     4321 cgacgaggag ctggaggaac gcgatggtcg tggtcatgag gcgcgaggcc accgaggccg
     4381 acatcgagct ggtctccgaa cgcgtccgcg ccgccggagg cgaggcgttc gtctcgcgcg
     4441 gcgcggtcca cacgatcgtc ggcctcgtcg gcgacaccga gcggttccag gcgatcccct
     4501 gggggcagct ccccggcgtc gaccacgtga tccaggtcgg caagccgtac aagatggtcg
     4561 ccgccgatct tcaccccacc ccgacgaccg tgaacgtcgg ctcgacgccg gtcggtcgtg
     4621 gcacgttcac cctgatcgcc gggccgtgcg cggtcgagag cgaggagcag gcgatgctcg
     4681 cgaccaaggc ggctcgggca gccggcgcga cgatcctgcg cggcgacgtg tacaagccga
     4741 ggacgtcgcc ctactcgttc cagggtctcg gcgaacgggg gctcgaggtc ctggaggagt
     4801 gccggcgtca gaccgggctg ccgatcgtcg tggaggtcgt cgacgtctcg caggtggaac
     4861 acgtcgccgc ggtcgccgac tgcatccgcg tcggtacgcg gaacgcccag aacttcgagc
     4921 tcctgaagga ggtcgggctg tcccgccggc ccgtgatgct gaagcgcggg ctcgcgatga
     4981 cgatcgagga gtggctccag gccgccgagt acgtcgcgca gcgcgggaac tccgagatca
     5041 tcctgtgtga gcgcgggatc cgaacgttcg aaccggccac gcggaacacg ctcgacctcg
     5101 cggggatggc ggtcgcgcag accgagtccc accttcccgt gatcgtcgac ccgtcgcacg
     5161 cggtcggcaa gcggcacctg gtcgcgccga tgtcgcgtgc ggcgctcgcg gcgggggcgg
     5221 acgccgtgat gatcgacgtg caccccgatc ccgagagtgc tttgtgcgac ggcccacagg
     5281 cggtcacgcc cgacgagatg ctcgatctgg gtcgcgagct cgagcggctc gccgcggcgc
     5341 tcggccggcc gttcaaccac gccgagtagg gctcggaggc cgtcccggcc agaccccgca
     5401 ggttcatcgt gcgttccgga agcgcgcctt gcggcgacgc cgacaggcgc cttacggtgg
     5461 atccatcggc actgagcgtc acatgaccgg gaggacagcc atgaggatgc ggatcggccg
     5521 aaccttcttc gcgttggcga tcgcggcccc gttgatcgcg gtcgtggcgc ccgcggcccc
     5581 caccgcggtc gcgcaacccg cgtgtgaact cggaaacggc atcgagcacg tcgtcaacat
     5641 cgtgttcgac aacgtgcact tcttccgcga caacccgaac gtgccctcgg acatcgagca
     5701 gatcgacagc ctcaacgact tcatccagga caacggcgtc atgctctcga acaaccacac
     5761 gcccttgatc gcccacacgg ccgaggacag ccttgcgatc tacaccggtc tgtacggcga
     5821 ccgtcacggg atgccgatca gcaactccta ccggacctgg aacccgaacg gaaccgtcaa
     5881 cttcggcaac ggccctttcc agggagctag ctccttcctg tattggaccg acccgaacaa
     5941 cgacgggggc aaggacacca agcacgcgat gatctactcg gacagcgtcc ccgcgtccgc
     6001 gaccacgacg aatgtcgcca cgccggcccc ctgggtcccg tggacccgag cgggatgcga
     6061 cgtcggcgac gtctcgacgg ccaacatggt gctcgagaac ccgggcttcg atatccccaa
     6121 ggtcttcgga ccgaactctc cggagacggc tcagctcatc gccgacaccc cgccgccacc
     6181 cgctccggcg ttcttcaacc aggagaccgc cgactacgtc ggtctcgctg tgcattgcgc
     6241 gcaggggagt ccgttctgcg cggacgcgga ggcgatcaag ttcgggcagg tcacgccatc
     6301 tcccacggcg gtggacgacg tgctgccgga cgagcccgga ggctacacgg ggtaccaagc
     6361 cctgttcggc cataagtaca tcgcgccgca gctcggcgcg gggaacgtga gcctgacgca
     6421 caacggctat ccggtgacga acgcggccgg gaacctcgtc gacttgagcg gcggccagat
     6481 caacggagcg ttcctgccac caaacacgcc cggattccca ggcttcggcc cgatcgtggc
     6541 gaagcaaacg ctcgcgtaca tggccgacat gctcgaggcg gatgtgccag tcgtctacgg
     6601 ctatatcggc gacatccacg agcgcaaggc cgggcagtcc ggctgctcga cctcgacggc
     6661 cgtggctgcc ggatgggcgc tcggtcccgg ggacaactgc taccggcaga ccgccgagca
     6721 gtacgaccag gccttccaaa ccttcttcga tcgcctggcc gcggccggta tcacgaagga
     6781 caacacgctg ttcgtgttcg gcgcggagga gaacgaccag ttcgacggcg ccaacgtcgg
     6841 gcgggcgatc gagccgacgc agggcgggac accgggctgt ttgggcttca gcgccgacac
     6901 gtactgctac gcatcgggcc agatcggcga gatcaacacg aacctgcccg gtcttctcgc
     6961 cgctcagaag gggaacacga ctccgttcgc catcgagccg cagggcgccg tcatctatgt
     7021 caacggccac cccggaccca acgatccggc cgtccgccag ctcgagcggg acatcggcgc
     7081 cctcaccaat ccccacgatc cgtacacggg caacgacgac gaatcgatca cggcctacat
     7141 ggccggcagc gtggaacaag agatcctgca cctggtgaac gccgatcccg atcggacccc
     7201 gacgttcacc ctgttcccca aacccgacta cttcttcggc tctcccgtca cgtgtacgcc
     7261 ggcggaccag gcaccgtgcg catccaacac cttggccagc gcggcgcggt tcgcctggga
     7321 tcacgggtac tacgcgccca cgatcgacat cacgtgggcg ggtttcgtgg gacccggcgt
     7381 agcgaacctc cgtctggacg gaccagaagc cgacgaaggg ccggcgctcg aagatcccga
     7441 cggcggcggg acggtccccg agttcagcag ggtcgggacg tgggtcgatc tcccggacat
     7501 ccgtccgacg atcctgtggc tggtgggtct gcaggacagc tacgtcaacg acggccgagt
     7561 gctgagcgag atcctcaccg atccgaacgc cgcgatagcc ggctccaggt acacggacct
     7621 cgccgcgtgc tacaagcagg tcaacgccag cgtgggcgag ttcggtacga acacgctgat
     7681 cgccgacacg gccgctctca agagcggcgg gggcggcgac ggattgttca agtcgacgca
     7741 gacgcggctg tccaagctgc tatcccagcg ggacgcgctg gcgcccgagc tcaacaacag
     7801 gctgttcgac gcggcgttcg acgatgttgc cttgccggcc gcgacgaatc tccttgatcg
     7861 gtgcgggtcg ctgctggaac aggccgagga gctcgcgtcg ggcgagtagg cgagctcgct
     7921 cttagccggg gaggacgggc gtgagctcgg cgatgtcgca cgagccgcgc cagcgcgcga
     7981 gccactcgct cggggtcccc gcgcggctct tgcgcttgtc cacgaggagc ttggctgcct
     8041 cttcccactc ggggccctgt aggacgcgcg aggccgcgtg gaactcggtc gcagcggtgt
     8101 cgcggccctt gcgccgggtc acgacgacga gttccgacgc gccggggatg cccggcacgc
     8161 tctgatcctc ggggccgggc ttccgctggg acagcacgaa gatcctgccg ttgcggtaga
     8221 cgaaccagga cgggatcagc ttgcgccgac cgttcgcctc gacgccgacc cagatcacgt
     8281 ccgaccgctt gaggccgtcc tcgatcacct tggggagctt cgccgacgcg agctggacca
     8341 ggaccgggac ctcgatctcg ccgacgatct cctcgtcgat gacgaacgac acgacgtagg
     8401 tcccggtctc gtcgaacgag gcgtccgtca ccgtgtctgt ctcgaccgtc agatccatcg
     8461 agccgatcat gcggcgaacc gcgggacccc agcgatgcac caggcgcccg gacggtccgt
     8521 agaagtggat ctcctcggtc accatgcccg tcccgacctt ccacgcgcgg gtgacgacga
     8581 agggaagcgc cgtacctggc gagccgagca ggtagacgcc cgggggaccc tgttcgccgg
     8641 aggtggccca gacacgctgg tccacgacgt tggcggagac g
//
LOCUS       DATBRK010000111        17180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_15030, whole genome shotgun sequence.
ACCESSION   DATBRK010000111 DATBRK010000000
VERSION     DATBRK010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17180
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15030"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VIH70_03335"
     CDS             <1..388
                     /locus_tag="VIH70_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115426.1"
                     /translation="SVAGAAGAGGVGGPAFYVDGTLYRTVGTPTDLTGTGAPASSFDT
                     IYEFFGAQPYNVATAAPGDPGYNGGRWRVHGLTFTSYATALSAYDANLSGDFDSEAEV
                     TAAIAGGAATDIGIVKSFVCPVIKIH"
     gene            477..1157
                     /locus_tag="VIH70_03340"
     CDS             477..1157
                     /locus_tag="VIH70_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8115427.1"
                     /translation="MPETVLVVDDDPTTRDVIVRYLEREGFEALEAEDGGRARAVIES
                     AEPNLVILDLMLPGIDGLSVIRWMRSTGWTPVIMLTALGEETDRIVGLELGADDYVTK
                     PFSPRELVARVRTVLRRSGPSAPAVERLETGDLVLDAAAREVRKGDAEVRLTAREFDL
                     LWFLASHPRRVFSRDQIMSRVWGYDDGLDTGTVTVHVRRVREKIEDDPSEPRHLETVW
                     GVGYRFTP"
     gene            1154..2227
                     /locus_tag="VIH70_03345"
     CDS             1154..2227
                     /locus_tag="VIH70_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8115428.1"
                     /translation="MSELALAAAAGILTVGLLAAIALGRFRTVHARLLGLTLIVGALP
                     IAAVVLSGVVMFDMHADAVLIAVAIASSVVAIGGALLVTRSISRPLGELRASTTRVAR
                     GDFSTRVGRRGPAELSEVAAAFNEMTSRLEELFDARRQLVAWASHDLRAPVSSLQAML
                     EAIEDGVVQPEHYLPAMRQQVHALRVLIDDLFELAQIDADDTAFETYGVPVGPLVESS
                     VEAFAAEASARRISLERRVATGLPDVEVSPEKIERVLRNLLANAVRHTPPEGSIVVGA
                     ERVNGTVVVAVEDTGEGIPSGAARTMFDRFWRGDPARDRSRDGAGLGLAIAKGLVERH
                     GGRIWAEEREGGGARIAFTLPIA"
     gene            complement(2382..2885)
                     /locus_tag="VIH70_03350"
     CDS             complement(2382..2885)
                     /locus_tag="VIH70_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115429.1"
                     /translation="MSEPPFDPLTSLRTLLERGVRFVLIGGYAGALRGSPVITGDLDV
                     CYARDDDNLERLADALGSLDAHLRGAPPDVPFLLDARTLRAGDHFTFGTSVGALDILG
                     TPSGTKGFADLDANATDEVVDGLTVRVASIEDLIRMKRAAGRPKDLIALEWLAAVRDE
                     IEDRGEG"
     gene            complement(2882..3196)
                     /locus_tag="VIH70_03355"
     CDS             complement(2882..3196)
                     /locus_tag="VIH70_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8115430.1"
                     /translation="MDARSIVLEARFGAGLSQRELARRSGVPQAAISRIERGFVSPRT
                     ETLDRLLRACGRDVGLIVRPGTGLDRSLIRERLRLTTAERARLAVREWENTRAFDRAV
                     RR"
     gene            complement(3222..4040)
                     /locus_tag="VIH70_03360"
     CDS             complement(3222..4040)
                     /locus_tag="VIH70_03360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017307293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8115431.1"
                     /translation="MIPLRDANPTKRFPVVTLALIAANVAVFLLWQPSFGTQLEQQTF
                     YYCHAEIPWEVSHQENLAQGGLEARVALDADLGPGAGADIQPFLRRECPTKSWWLAIF
                     VAMFLHGGWFHIAGNMLFLWVFGNNVEDRLGYVAYPLFYVLGGLAASALQLAFGANST
                     IPSLGASGAIGGILGAYIVLFPHARVTTLVIFFFITVVEIPAGVVLAIWFVLQLFSGV
                     GQLGAEVGGGVAYWAHVGGFAFGAVVAWLFYRRRGGLGPRAYDVGRGPGDPFGR"
     gene            4097..5740
                     /locus_tag="VIH70_03365"
     CDS             4097..5740
                     /locus_tag="VIH70_03365"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEY8115432.1"
                     /translation="MSGRRASDLLVSCLEAEGVPYVFGVPGEETVDLNESLSRSTVRF
                     VPTRHEQAAAYMADAYGRLTGRAGVCLATLGPGATNLVTGVADAFLDRAPIVVLTGQG
                     DLERMHKESHQYIDVLAIMRPITKWNARVSDPAIIPEVVRKAFKVAEAEKPGSTHIEL
                     PEDVMAAETRGEPLARTEPVHPDASSADIARAAELIRHAEHPIALAGNGAVRGRAANT
                     VRALTRRTGIPVATTFMAKGLMDPDDELALGAVGLQPGDYRMTGFDEADLVIAIGYDL
                     VEHAPRHWNPNKDKAIVCIDTVPAEIDEHFIPAVELVGDVSRSLERLTRKAERASHPA
                     GSPRLRDAVRERLDRADDDPESPLQPPRVLVELRRALGKRDILVSDVGLHKLWIGRMF
                     PAYEPDTVLISNGLAAMGFALPAAIATKLVHPDRNVVAVSGDGGFMMNAQELETAVRL
                     GTAVVNVVWEDGGFGSIAWKQEKRFGRHFGTDFGNPDLVRFAESFGIPAWRCDTASDF
                     AKRLEEALATAGPSLIAIPIDYSPDVAIVEELEDEPKAT"
     gene            5795..6748
                     /locus_tag="VIH70_03370"
     CDS             5795..6748
                     /locus_tag="VIH70_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8115433.1"
                     /translation="MDGGAEPDEEALDAYSRVVTSVAERLIPSVASLRVRQRVRGGSL
                     DGAGSGVVITPDGFALTSAHVVGGTDRGSASFVDGREIPFDVVGRDELSDMAVLRLQS
                     RSDLEPAELGEAARLRVGQLVVAIGNPMGFAGSVTAGVVSALGRALPVQAGRVVENVI
                     QTDAALNPGNSGGALADGRARVVGINTAVAGVGLGLAVPIDDSTRRIMHALMTSGRFQ
                     RAYIGIAGGGRPLPPRVAAAVGRDQGVEVMEVVAGSPAARAGIRSNDLIVELDGVPIS
                     DARDLQRLMVEDVIGRSVEALVWRDGELRTIMIHPAELTAA"
     gene            6745..7362
                     /locus_tag="VIH70_03375"
     CDS             6745..7362
                     /locus_tag="VIH70_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8115434.1"
                     /translation="MNDRKAGARRWFDRRAASYESGLTSRWRDPVQRASLDALHLGAE
                     DRLLDVGCGTGAASRSASSVAASVVGIDLSPEMVRQAQALANGIANVHFEIADSEHLP
                     FGDGTFTAVLCSNSFHHYPDPGRAVREMARVLASGGRIVLGDACSDSAAARVADAFLR
                     RFEPGHVRLYRSHELGSFVRDAGLSRVELRRLSGGGFAIVRGVAA"
     gene            7449..10334
                     /locus_tag="VIH70_03380"
     CDS             7449..10334
                     /locus_tag="VIH70_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8115435.1"
                     /translation="MTTPTPCQVCGQSLPEGAGFCPNCGSPVGSPLGTAERKVVTVLF
                     ADLVDSTGLAQRLDPERAREILGYFFEAASEELLALRGRAEKFIGDAVMAVFGMPTVH
                     EDDALRAVRAGLAIRGRTRRLSQALGLPEPLEVHVGVESGEAATGLGPAGQLLVTGPV
                     VNAAARLQAAAGPGEVLAGETTVALTANEVSYGVRREATAKGFQGDTIAGFEVEGLTP
                     RSARRTIPFVGRSSELAILRESLHRATDTGHPVLVSVLGEPGIGKSRLADELVALVED
                     DVPVLAGRARPFTDTATFAPAATIVAELAGLEEDDPPEKARRRLRELAERYVDPDESA
                     HVVERLALLFGLADRPSQASFVQDVQSGFVALVDGLARDTAAVLVFEDAHELRPAMLE
                     LIERLGSPTRDGHRKVLVLALARTTLLEQRPAWGTTTANAVRLQLEPLSAEESIDLAR
                     QASGRRIDDAEATEIAERAGGNPFFIIETTGMLLPDPDGRREAAPRPSLPPTVQAVVA
                     ARLDHLEPRLRELTRRASSFFVSFDLHELRTIDPEVTEDELRRLEEAEILVHEDPGHA
                     VIRWRVRHATVKEVAYASLPKRERVRLHQLIADRLFADSHPAWAADHLELAAIASLDL
                     DRDDRSVAERAADALLDAGDRARRRMESRTAIDRYERALGLAGSEGTWGVREARALAG
                     IGEARYWLGEYPAATDVLGRAVVLGREVDDPFTLSLALRFLGDIAINVEADLDKAETL
                     LDESLAAAEAMDEPWACTRTLLFAGWVPWTRGDHEAAEKIWRRALEVADPDDGWARVR
                     ALNSLSINRTGTPGRSGVSPEEELRQALELSDEASALAEEIGDQFSIAMTTVQRARIL
                     DDQGKFEESLPCFDRAIGVFEDLGARWELGDATAERGIAKRELGRLDEAEEDLRKAIR
                     ISEELGERQLASWTWRALAKVSERRGDGAEAERQLRRSREAEAQGPR"
     gene            10432..11256
                     /locus_tag="VIH70_03385"
     CDS             10432..11256
                     /locus_tag="VIH70_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115436.1"
                     /translation="MEEPRVPEPPGSPRLLRPRSVGELLTDAFELYRRHWQNLFMIVA
                     VIVIPLSFLQVLVGDFWIRQGLSEEELRNGVEVTVNGAFVGAIFASFVVAITSILMWT
                     VLTGAITRAAAGTFLARDLDIGESYRYGFARFWSIVLVGLLSGLAIAVGFLLLIVPGF
                     IVLTFLTCAIPALVIEDKRGREALRRSWNLVRGRGWPVFGTILVAFLINGIVNSLLTS
                     PFGDNWAARSIGASIASILTMPYMALVSVFIYLDVRVRKEQYSAAQLERDLASSGS"
     gene            complement(11275..14583)
                     /locus_tag="VIH70_03390"
     CDS             complement(11275..14583)
                     /locus_tag="VIH70_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115437.1"
                     /translation="MSIVIAIGVVLVAVGGAVALWRLCRLGAASDRWALIVISAGIVA
                     FITLGVMAEIEGLLQAMHPLGRTGRGVMAIIADPVRARATVDAWQNWFSQPYATASAV
                     TVARPPLAPRTAASWFLGIDSFAFVPAYVLFLGVLFRRAQKVVKDEAHRRATLVRETD
                     QGTKKQKVKQKAREDMASSTLTGFQRTAAIAAGILALVPFFDLLENGMLWRFVDRRVE
                     ACPSGLCEDVNVPSDGFVQLLRVVSYTKLILVAGAIVVALLPLAAWVRLGTDWFRSLR
                     PALRRLRGPIVVLVVFFVLMWANPQTKDVFRGWDLPRGALAFLAAVAAAYVTWSIGRR
                     VTRVRRGKDEAGHPLQGRDPRQVRWWMFGAGVALFLVGFTGIWFDLGPRGLMIPGLFL
                     GALGLFDPAGEDIVVAAPVEVSAPIRDQVPALLAAAWPIALSYFVARSLFPLTMYDHA
                     GSFLYVTVVYVSWTIVVPATSIAFFYYGFLYAAARASAVRREPRPASGRPSPPGSIVG
                     RAVSGAIMVATPTDTRRHPYEVDALIVAGVLLWWRVLVNPWAVGQGFGAIALVVAFFA
                     VSTAAVGWVSLFVERSEPPGLFKVLRVQRTPVFLLLLLWLVLTSVLPLIGREDFHDVR
                     TIRAGVDRSLVSMGTNDLFARWIANQPGLAADGAPGVPRQAVPLILVATEGGGVKAAT
                     WTALVLDCVLSPDPIVDRCPGHDGPRLASVFAESGVSGGSVGLVEYTRYAADPALADR
                     SGDWIEQRLGADFVSPSIAWQLYIETPRAWLQFDAGLDRAEVLERGWEQTWVEEGRFG
                     LLDALLRGAPASEGPLAEGFRSLFANDTNVPLLLLNGFSVQDGCKFVTTPIDGNGPES
                     DSLNCVVAHADVEVRADPSLGRPGFLSGSRVLTDLLCKPDDDVRLSTAALLSARFPYV
                     SPSGRLLSCGEESRRAINVVDGGYGENTGAGSVLELWQSLRPLVDEYNGDPSHGACIV
                     PVLLQIQSGYGPPADMPSTGQAELAVPPKGILRAPNGFTVWQRNAAQLAVLDPLPFTG
                     SATQKARQGVYAHVYPYAHPGAEAPLGWTLSDEARNDLRAQLSATGNVEALGGLEVFL
                     NDPGTCAPGDQATA"
     gene            complement(14698..16032)
                     /locus_tag="VIH70_03395"
     CDS             complement(14698..16032)
                     /locus_tag="VIH70_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8115438.1"
                     /translation="MTEVAETRYAKTLDGGYIAYKLLGDPTSENEVAVIGSIAANVEL
                     FFEFEPAARSWLELASFSRLSLHDRRGTGLSDDMGGLPDLETRAADLLAVLDAAGLRR
                     PILLASADGGMVAALLAATHPDRVAGLVWFFAQARSVKTRGWSFGRDPDDVDRLAREA
                     ERAWGTEDFVRRMYAHAEMDDDVIRFLARMQRHSCGPATAVGFIRLLHDYDVREILPT
                     LRIPVLALGTEGYPGEWVVEQTRATAALIPGAVCRSMPARASRLWAPAVIEATFDFCG
                     IQRPPPELDSILSTVLFTDIVGSVEKQAEFGDHAWKELVLQYRSIVRTTLERWRGREN
                     DTAGDGFYAAFDGPARAIRCALEVAERVRDLGIEIRAGVHTGECELIEDKCSGLTVSI
                     GARVAAIGGAGDVLVSQTVKDLVPGSGLQFDEAGEHQLKGVPGRWRLHKVIG"
     gene            complement(16348..>17180)
                     /locus_tag="VIH70_03400"
     CDS             complement(16348..>17180)
                     /locus_tag="VIH70_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8115439.1"
                     /translation="RDGSANGRIEVKVGIEWLPLASIGLLRDASALARDLGTGIHIHL
                     NESVGEVEISKEKFGRRPTEVAYECGILGSSTVAAHCVWLSDTEIGLMRDTGTHISHN
                     PTSNAKLGNGVARLLDYLRAGINVGLGHDSTEGVNTGDMFEVMKFASLMQRAVRMDAN
                     VLQAGDLLRMATRNGSDALRHDTGMLAEGKKADVIVIELDDVGFTPLVPGNRNQLYSH
                     LAFASHGRAVSSVVIDGAVVMRERELLTVDEEEIRRKANEAFVRILDRAHVGSVQTIV
                     "
BASE COUNT         2632 a         6168 c         5977 g         2403 t
ORIGIN      
        1 gtcggtcgcg ggtgcggcgg gagccggtgg cgtcggcggc ccggcgttct acgtcgacgg
       61 gacgctgtac cggacggtcg gcacgcccac ggacctgact gggaccggcg cgccggcgtc
      121 gagcttcgac acgatctacg aattcttcgg cgcccagccg tacaacgtgg cgacggccgc
      181 gcccggcgac cccgggtaca acggcggccg gtggcgcgtg cacggcctca cgttcacgag
      241 ctacgcgacc gcgctgtcgg cgtacgacgc gaacctcagc ggcgacttcg acagcgaggc
      301 ggaggtcacc gccgcgatcg cgggcggtgc cgcgaccgac atcggcatcg tcaagagctt
      361 cgtctgcccc gtgatcaaga tccactgatg gatccgttcc gggcggcgcg gcgtcccccg
      421 aggcgccgcg ccgtcccgaa cccgttcccg cggagcgggg atatcctcga ctgcacatgc
      481 ctgagaccgt gctggtcgtc gacgacgatc cgacgacccg ggacgtgatc gtccgctacc
      541 tcgagcgtga ggggttcgag gcgctcgagg ccgaggacgg cggccgggct cgagcggtca
      601 tcgaatccgc cgagccgaac ctcgtgatcc tcgacctcat gctcccgggg atcgacggcc
      661 tctcggtgat ccgctggatg cgctcgaccg gctggacgcc cgtgatcatg ctcaccgcgc
      721 tgggcgagga gaccgatcgc atcgtcggtc tcgagctggg agccgacgac tacgtcacca
      781 agccgttctc gccccgcgag ctcgtggcgc gcgtcaggac ggtgctccgg cgttcggggc
      841 cgtcggcgcc ggcggtcgag cggctcgaga ccggcgatct cgtcctggac gcggccgcgc
      901 gcgaggtccg aaagggcgac gccgaggttc ggctgaccgc cagggagttc gacctgctgt
      961 ggttcctcgc gagccatccc cgacgggtgt tctcccgcga ccagatcatg agccgggtct
     1021 ggggctacga cgatgggctc gacacgggga ccgtgacggt ccatgtccgg cgcgtccggg
     1081 agaagatcga ggacgacccg agcgagcccc gacatctcga gacggtctgg ggcgtcggct
     1141 accggttcac gccgtgagcg agctcgcgct ggcggcggcc gcgggcatcc tcaccgttgg
     1201 tctgctcgcg gccatcgcgc tcggtcggtt ccgaacggtc cacgcccgtc tgctcggact
     1261 caccctcatc gtcggcgcgc tcccgatcgc ggccgtcgtg ctctctggcg tggtgatgtt
     1321 cgacatgcac gcggacgccg tgctgatcgc ggtcgcgatc gcttcgtcgg tggtggcgat
     1381 aggcggcgcg ctgctcgtga ctcgctcgat ctcgcgcccg ctcggcgagt tgcgcgcgtc
     1441 gacgacgcgg gtcgcgcgcg gcgacttctc caccagggtc ggccggcgcg ggccggcgga
     1501 gctctcggag gtcgcggccg cgttcaacga gatgacctcg cggctcgagg agctattcga
     1561 cgcccggcgg cagctcgtgg cgtgggcgag ccacgacctg cgggccccgg tctcctcgtt
     1621 gcaggcgatg ctcgaggcga tcgaggacgg ggtcgtccag cccgagcact acctgcccgc
     1681 gatgcggcaa caggtccacg cgctccgcgt gctcatcgac gacctgttcg agctcgcgca
     1741 gatcgatgcg gacgacaccg cgttcgagac gtacggcgtg ccggtcggac cgttggtcga
     1801 gtcctcggtc gaggcgttcg cggcggaggc gagtgcccgc cggatcagcc tcgagcgccg
     1861 ggtggcaacg gggttgcccg acgtcgaggt ctcgcccgag aagatcgagc gcgtgctccg
     1921 gaacctgctc gcgaacgccg tccgccacac gccgcccgag ggctcgatcg tcgtgggcgc
     1981 ggagcgcgtg aacggcacgg tcgtggttgc cgtcgaggac acgggcgagg gcatcccgtc
     2041 gggggcggcc cggacgatgt tcgatcgctt ctggcgcggc gatcccgccc gcgaccggag
     2101 ccgcgacggt gccgggctgg gtctcgcgat cgcgaagggc ttggtcgagc ggcacggcgg
     2161 ccggatctgg gccgaggagc gagagggcgg cggcgcgagg atcgcgttca ccctgcccat
     2221 cgcatgacgg cgggtcgtca gggttctgac agacagtgtt gtgacgccgg gcgtcatcgg
     2281 aaacgctcgg tcgtcagggt tctgacgggc agaaccctga cgccgggcga aaacccgcgg
     2341 gacctcccct cggccccgac ccacgtcggc ccgatcccgc cctacccctc gccgcggtcc
     2401 tcgatctcgt cccgcacggc cgccagccac tcgagcgcga tgagatcctt cgggcggccg
     2461 gccgcgcgct tcatgcggat cagatcttcg atcgacgcga cccgcaccgt gagtccgtcg
     2521 accacctcgt cggtcgcgtt cgcatcgagg tcggcgaacc ccttcgtccc cgacggcgtg
     2581 ccgaggatgt ccagcgcacc caccgacgtg ccgaacgtga agtgatcgcc ggcgcgcagc
     2641 gtgcgggcat cgagcaggaa cggcacgtcg gggggggcgc cgcggaggtg ggcgtcgagc
     2701 gatccgagcg cgtcggccag tcgctcgagg ttgtcgtcgt cacgcgcgta gcagacgtcg
     2761 agatcgccgg tgatcaccgg tgaacctcgg agggctcccg cgtatccacc gatcaggacg
     2821 aaccggacgc cccgctcgag cagcgtccgc aacgacgtca acgggtcgaa cggaggctcg
     2881 ctcaccggcg cacggcccgg tcgaacgcgc gcgtgttctc ccactccctg acggccagcc
     2941 gcgcacgctc ggcggtcgtg agccggaggc gttcccggat caggctgcgg tccaggccgg
     3001 tcccgggtcg cacgatcagc ccgacgtccc ggccgcaggc tcgcaggagg cgatccaggg
     3061 tctccgttcg cggcgacacg aaaccgcgct cgatgcgcga gatcgccgcc tgaggtaccc
     3121 ccgatcgccg ggccagctcg cgctgcgaca gccccgcccc gaaccgggcc tcgagcacga
     3181 tcgaacgggc gtccaccagc gaaatgatat cgcaaacgac atcatcgccc gaagggatcc
     3241 cccggtcctc gcccgacgtc atacgccctc ggcccgagcc caccccgccg ccggtagaac
     3301 agccaggcca cgacggcgcc gaaggcgaac ccgccgacgt gcgcccagta cgcgaccccg
     3361 ccgcccacct ccgccccgag ctgacccacg ccgctgaaca gctggagcac gaaccagatg
     3421 gccagcacca cgcccgcggg gatctcgacg accgtgatga agaagaagat cacgagcgtc
     3481 gtcacccggg cgtgcgggaa cagcacgatg tacgcgccca ggatcccgcc gatcgcgccg
     3541 gacgcgccga ggctcgggat cgtcgagttc gccccgaacg cgagctggag cgccgacgcc
     3601 gccagcccgc cgagcacgta gaagagcggg taggccacgt agcccagccg gtcctcgacg
     3661 ttgttcccga agacccacag gaacagcatg ttgccggcga tgtggaacca acccccgtgc
     3721 aggaacatcg cgacgaagat cgcgagccac cagctcttgg tcggacactc ccgccgcagg
     3781 aacggctgta tgtccgcccc cgcacccggg ccgagatccg cgtcgagcgc gacccgcgcc
     3841 tccagacctc cctgcgcgag gttctcctgg tgcgagacct cccacgggat ctcggcgtgg
     3901 cagtagtaga acgtctgctg ctccagctgc gtgccgaacg aaggctgcca cagcaggaac
     3961 accgcgacgt tcgcggcgat gagcgcgagg gtgacgacgg ggaagcgctt ggtggggttc
     4021 gcgtcccgga gcgggatcac gggtcggagt atgccagggt cgggtacgcg gagcagcccc
     4081 gtagactcgg ctcgacatgt ccggtcgaag ggcctccgac ctgctcgtct cctgcctgga
     4141 ggccgagggc gtcccctacg tgttcggcgt gccgggagag gagaccgtcg acctcaacga
     4201 gtcgctgtcc cgctcgaccg tccgcttcgt tccgacgcgg cacgagcagg cggccgcgta
     4261 catggccgac gcctacggcc gcctcacggg tcgggcgggc gtctgcctcg cgaccctggg
     4321 gcccggggcg acgaacctcg tgaccggggt cgccgacgcg ttcctcgatc gcgccccgat
     4381 cgtcgtcctc accggccagg gcgatctgga acggatgcac aaggagtccc atcagtacat
     4441 cgacgtcctg gcgatcatgc gcccgatcac caagtggaac gcgcgcgtga gcgacccggc
     4501 gatcatcccc gaggtcgtcc gcaaggcctt caaggtcgcg gaggccgaga agccgggatc
     4561 cacccacatc gagctgcccg aggacgtaat ggcggcggag acgcggggcg agccgctcgc
     4621 ccggacggag ccggtgcacc ccgatgcctc gtccgccgac atcgcgcgcg ccgccgagct
     4681 catccgccac gccgagcacc cgatcgcgct cgcggggaac ggggcggtcc gcgggcgtgc
     4741 cgcgaacacc gttcgcgcgc tcacgcgcag gaccggtatc ccggtcgcga ccacgttcat
     4801 ggccaagggg ctgatggacc ccgacgacga gctcgcgctg ggggcggtgg ggctgcagcc
     4861 cggcgactac cggatgaccg gcttcgacga ggccgatctg gtgatcgcga tcggctacga
     4921 cctggtcgag cacgcgccgc ggcactggaa cccgaacaag gacaaggcga tcgtgtgcat
     4981 cgacacggtc cccgccgaga tcgacgagca cttcatcccc gccgtcgagc tcgtcggtga
     5041 cgtgtcgcgg agcctcgaac gactgacgcg caaggccgag cgagcgtcac accccgccgg
     5101 ctcgccccgc ctgcgagacg ccgtgcgcga gcgcctggat cgcgccgacg acgacccgga
     5161 gtcgccgctg caaccgccca gggtcctcgt cgagcttcgg cgcgcgctcg ggaagcggga
     5221 catcctcgtc tccgacgtcg ggctccacaa gctctggatc ggccggatgt tccccgcgta
     5281 cgaaccggac acggtcctca tctcgaacgg cctggccgcg atgggcttcg cccttccggc
     5341 ggccatcgcg accaagctgg tccacccgga ccgcaacgtg gtggcggtga gcggcgacgg
     5401 cgggttcatg atgaacgcgc aggagctcga gaccgcggtc cggctgggga ccgccgtcgt
     5461 caacgtcgtc tgggaggacg gcgggttcgg ctccatcgcg tggaagcagg agaagcggtt
     5521 cgggcgccac ttcgggacgg acttcggcaa cccggacctc gtgcggttcg ccgagtcgtt
     5581 cggcatcccc gcctggcgat gcgacacggc ctccgacttc gccaagcggc tcgaggaggc
     5641 gctcgcgacg gccggcccga gcctcatcgc cattccgatc gactactcgc ccgacgtcgc
     5701 catcgtcgag gagctcgaag acgagcccaa ggcgacctga tcgatccgag accgatgggt
     5761 ctcgttcagc gtttcctgcg agaagatgga gggcatggac ggcggcgcgg agccggacga
     5821 ggaggcgctc gacgcctact cgcgcgtggt cacctccgtc gccgagcgtc tgatcccctc
     5881 ggtcgccagc ctccgggtgc ggcaacgcgt gcgtggaggc agcttggacg gcgccggctc
     5941 gggcgtggtg atcacgccgg acggcttcgc cctgacctcg gcccacgtcg tgggcgggac
     6001 ggatcgcggg tcggcgtcgt tcgtggacgg gcgcgagatc ccgttcgacg tggtcggccg
     6061 cgacgagctc tcggacatgg cggtgctccg cctccagagc cgcagcgacc tcgaaccggc
     6121 ggagctcgga gaggcggcga ggctccgcgt cgggcagctc gtcgtggcga tcgggaaccc
     6181 gatgggcttc gcgggatccg tcaccgccgg cgtggtgagc gccctcggcc gcgcgctccc
     6241 ggtgcaggcg ggccgcgtcg tcgagaacgt catccagacc gacgccgcgc tgaaccccgg
     6301 gaactcgggc ggcgcgctcg ccgacgggcg cgctcgcgtc gtcggcatca acaccgccgt
     6361 cgccggggtc gggctcggcc tcgcggtccc gatcgacgac tcgacccgcc gtatcatgca
     6421 cgcgctcatg acgtcgggcc ggttccaacg cgcgtacatc gggatcgccg gaggcgggcg
     6481 ccccctgccg ccgcgcgtgg ccgccgcggt gggccgcgac cagggcgtcg aggtcatgga
     6541 ggtcgtcgcg ggcagcccgg ccgcccgagc cggtatccgg tccaacgacc tgatcgtcga
     6601 gctcgacggc gtgccgatct cggacgcgcg cgacctgcag cggctgatgg tcgaggacgt
     6661 gatcggccga tcggtcgagg cgctggtttg gcgcgacggc gagctccgga cgatcatgat
     6721 ccaccccgcc gagctcacgg cggcgtgaac gataggaagg ccggtgcgcg ccggtggttc
     6781 gaccggcggg ccgcgagcta cgagtcgggg ctcacctcac ggtggcgcga ccccgtccag
     6841 cgcgcgtcgc tcgacgcgtt gcatctcggc gccgaggatc gcctcctcga cgtcggatgc
     6901 ggtacggggg cggcgtcgcg ctcggcgtcg tcggtcgcgg catcggtcgt cggcatcgac
     6961 ctgtccccgg agatggtccg gcaggcgcag gcgctcgcga acgggatcgc gaacgtgcac
     7021 ttcgagatcg cggactccga acacctgcct ttcggcgacg ggacgttcac cgccgtactg
     7081 tgctcgaact cgttccacca ctaccccgat cccggccggg cggtgcgcga gatggcccgc
     7141 gtgctcgcgt ccggcgggcg gatcgtccta ggggatgcgt gctcggacag cgccgccgcg
     7201 cgcgtcgccg acgcgttctt gcgacggttc gagcccggtc acgttcgtct gtatcgctcg
     7261 catgagctgg ggtcgttcgt tcgggatgcc gggctgtcgc gcgttgagct ccggaggctc
     7321 tccggtggcg ggttcgcgat cgtccgaggg gtcgccgcct gaccctcggg tcgcctccga
     7381 taccgcttcc aatatcgccg cgttgcggga atatcgccgt catccccaga cctatccttc
     7441 aaagagccat gacgaccccc accccctgcc aggtctgcgg ccagtcgctc cccgagggcg
     7501 caggcttctg cccgaactgc ggctcccccg tcggctcccc cctcggcacc gcggaacgca
     7561 aggtggtcac ggtgctgttc gccgacctcg tcgactccac cggcctcgcg cagcgcctgg
     7621 accccgagcg cgcccgcgag atcctcggct acttcttcga ggccgcgtcc gaggagttgc
     7681 ttgcgctccg cggccgggcg gagaagttca tcggtgacgc cgtgatggcg gtcttcggca
     7741 tgcccaccgt ccacgaggac gacgcgctcc gcgccgtgcg cgcgggtctc gcgatccgtg
     7801 gccggacccg ccgcctgagc caagcactcg gcctgcccga acccctcgag gtccacgtcg
     7861 gcgtcgagtc gggcgaggcc gccaccggtc tcgggcccgc gggccagctc ctggtcaccg
     7921 gcccggtcgt gaacgccgcg gctcggctcc aggccgccgc cggacccggc gaggtgctgg
     7981 ccggcgagac caccgtggcg ctcacggcca acgaggtctc gtacggggtc cggcgtgagg
     8041 ccaccgccaa gggcttccag ggggacacga tcgccggctt cgaagtcgag gggctcacac
     8101 cccggagcgc ccgcagaacg atcccgttcg ttgggcgctc gagcgagctc gcgatcctgc
     8161 gcgagagcct gcaccgcgcc accgacaccg gacatccggt cctcgtcagc gtcctcggcg
     8221 aacctggcat cgggaagtcg cgcctggccg acgagctcgt cgcgctcgtc gaggacgacg
     8281 tcccggtgct cgccgggcgc gcgcggccgt tcaccgacac cgccacgttc gcacccgccg
     8341 cgaccatcgt ggccgagctc gcgggcctgg aggaggacga cccgcccgag aaggccaggc
     8401 gccgcctgcg cgagctcgcc gagcgctacg tcgaccccga cgaatccgcc cacgtcgtcg
     8461 aacggctcgc gctcctgttc ggcctggccg accggccgtc gcaggcgtcg ttcgtgcagg
     8521 acgtccagtc cgggttcgtc gcgctcgtcg acggcctcgc gcgcgatacc gccgccgtcc
     8581 tcgtgttcga ggacgcgcac gagctccgcc cggcgatgct cgagctgatc gaacgcctgg
     8641 ggtcgccgac ccgcgatggt caccgcaagg tcctcgtcct cgcgctcgcc cgcaccacgc
     8701 tgctcgagca gcggccggcg tggggcacga cgaccgcgaa cgcggtccgg ctccagctcg
     8761 agccgctctc tgccgaggag tcgatcgatc tcgcgaggca ggcatcgggc cgccgcatcg
     8821 acgacgccga ggcgaccgag atcgccgaac gcgccggcgg caaccccttc ttcatcatcg
     8881 agacgaccgg catgctgctc cccgaccccg acggacggcg cgaggcagcg ccgcgcccga
     8941 gcctgccgcc aaccgtgcag gccgtcgtcg cggcccggct cgatcacctc gaaccccggc
     9001 tgcgtgagct cacccggcgc gcgtcgtcgt tcttcgtctc gttcgaccta cacgagctcc
     9061 ggacgatcga ccccgaggtc accgaggacg agctccgccg gctcgaggag gccgagatcc
     9121 tcgtgcacga ggacccgggc cacgcggtca tccgatggcg cgttcggcac gcgacggtca
     9181 aggaggtcgc gtacgcgagc ctgcccaagc gggagcgggt gcgcctgcac cagctgatcg
     9241 ccgaccggct gttcgcggac agccacccgg cgtgggcggc cgaccacctc gagctggccg
     9301 cgatcgcctc gctcgatctg gaccgggacg accgatcggt ggccgagcgc gcagccgatg
     9361 cgctcctgga cgcgggcgat cgcgccaggc gccgcatgga gagccggacg gcgatcgacc
     9421 gctacgagcg ggcgctcggg ctcgcgggct ccgagggcac gtggggcgtc cgcgaggcgc
     9481 gcgcactcgc gggcatcggc gaggcccgat actggctcgg cgaatacccg gcggccaccg
     9541 acgtgctcgg ccgggcggtg gtgctcggcc gggaggtcga cgacccgttc acgctctcgc
     9601 tcgcgctgcg gttcctgggc gacatcgcga tcaacgtgga ggccgacctc gacaaggccg
     9661 agacgctgct cgacgagtcg ctcgcggcgg ccgaggcgat ggacgagccg tgggcgtgca
     9721 cccgcacgct gctgttcgcc ggctgggtgc cgtggacgcg cggcgaccac gaggcggccg
     9781 agaagatctg gcgccgcgcg ctcgaggtcg ccgacccgga cgacggctgg gcgcgcgtgc
     9841 gggcgctcaa ctcgctgtcg atcaacagga cgggcacccc cggtcgctcc ggcgtctcgc
     9901 ccgaggagga gctgcggcag gcgctcgagc tgagcgacga ggcgagcgcc ctcgccgagg
     9961 agatcggcga ccagttctcg atcgcgatga cgacggtgca gcgagcccgg atcctggacg
    10021 accagggcaa gttcgaggaa tcgctcccgt gcttcgaccg cgcgatcggc gtgttcgagg
    10081 acctcggcgc gcggtgggag ctcggcgacg cgaccgccga acgcggcatc gccaaacgcg
    10141 agctcggccg cctggacgag gccgaggagg atctccgcaa ggcgatccgc atctccgagg
    10201 agctcggcga acgccagctc gcgagctgga cctggcgcgc actcgccaag gtctcggaac
    10261 gccgaggcga cggggcggag gcggagcggc agctacggcg ttctcgtgag gcggaggctc
    10321 aggggccgcg gtagccaact cgaaccgcgc accgacgccg ggtagagtgg ccctcgaggc
    10381 gtgcccgacg agccgcacgc ggtcggccgc acggctatgg gaggagatcc gatggaagaa
    10441 ccccgagtcc ccgaaccgcc cggcagcccc aggctgctcc gcccgcgcag cgtcggcgag
    10501 ctcctcaccg acgcgttcga gctctaccgc cggcactggc agaacctgtt catgatcgtg
    10561 gcggtgatcg tcatcccgct ctcgttcctg caggtgctcg tcggcgactt ctggatccgc
    10621 cagggcctca gcgaggagga gttgcgcaac ggcgtcgagg tcaccgtgaa cggggcgttc
    10681 gtcggggcca tcttcgcctc gttcgtggtc gcgatcacgt cgatcctgat gtggacggtc
    10741 ctcacgggcg ccatcacacg ggccgcggcc gggaccttcc tcgcccgcga cctggacatc
    10801 ggcgagagct accggtacgg gttcgctcgc ttctggtcga tcgtgctggt cggcctgctc
    10861 tccggccttg cgatcgcggt cggcttcctg ctgctgatcg tccccggctt catcgtgctc
    10921 acgttcctca cctgcgcgat ccccgcgctg gtgatcgagg acaaacgcgg acgcgaggcg
    10981 ctccgccggt cctggaacct ggtacgcggg cgaggatggc ccgtcttcgg gaccatcctc
    11041 gtcgccttcc tgatcaacgg gatcgtcaac agcctgctga cctcgccgtt cggggacaac
    11101 tgggcggccc gcagcatcgg cgcctcgatc gcgtcgatcc tcacgatgcc ctacatggcg
    11161 ctcgtgagcg tgttcatcta cctggacgtg cgcgtccgca aggagcagta ctcggcggcg
    11221 cagctggaac gcgacctcgc gtcgagcgga agctgagccc cgagcggcaa ggcgctacgc
    11281 ggtcgcctga tcgcccggcg cgcaggttcc gggatcgttc aggaacacct cgagcccccc
    11341 gagggcctcg acgttgcccg tggccgagag ctgcgcccgc aggtcgttcc tggcctcgtc
    11401 tgaaagcgtc cagccgagcg gcgcctcggc cccgggatgg gcgtaggggt acacgtgtgc
    11461 gtacacaccc tgccgcgcct tctgggtggc cgaacccgtg aacgggagcg ggtccagcac
    11521 cgccagctga gcggcgttgc gctgccagac cgtgaagccg ttcggcgcgc gcaggatgcc
    11581 cttgggcggg acggccagct cggcctggcc ggtcgacggc atgtcggcag gcggcccgta
    11641 gccgctctgg atctgcagca acacgggcac gatgcaggca ccgtgcgagg ggtccccgtt
    11701 gtactcgtcg acgagcgggc gtagggactg ccagagttcg agcacgctgc cggctcccgt
    11761 gttctcgcca tacccgccgt ccacgacgtt gatggcccgg cgcgactcct ccccgcaact
    11821 gagcagccga ccgctcggcg acacgtacgg gaaccgcgcc gaaagcagcg ccgcggtgct
    11881 caaccgcacg tcgtcatcgg gcttgcacag cagatcggtc agcacccggg agcccgagag
    11941 gaacccgggc cttcccaggg acgggtccgc ccgcacctcc acgtcggcat gggcgaccac
    12001 acagttgagg ctgtccgact ccgggccgtt gccgtcgatc ggtgtggtca cgaacttgca
    12061 cccgtcctgc accgaaaaac cgttcagcag cagaagcggc acgttcgtat cgttcgcgaa
    12121 cagggatcgg aagccctcgg cgagcggtcc ctccgaagcg ggcgccccgc ggaggagcgc
    12181 gtccagcaat ccgaaccgtc cctcctccac ccaggtctgc tcccagccgc gctcgagcac
    12241 ctctgcgcga tcgaggccgg cgtcgaactg gagccacgca cgcggcgtct cgatgtagag
    12301 ctgccaggcg atcgagggcg agacgaagtc ggcgccgaga cgctgctcga tccagtcgcc
    12361 ggaccggtcg gccagggcgg gatcggccgc gtagcgggtg tactcgacga ggccgacgct
    12421 gccgcccgag actccgctct cggcgaagac ggaggcgagt cgcggcccgt cgtgaccggg
    12481 gcaccgatcg acgatcgggt ccggcgagag cacgcagtcc agtaccagcg cggtccaggt
    12541 cgcggccttg accccgccgc cctcggtggc gaccaggatc agcggcaccg cctgcctcgg
    12601 cacgccgggg gcgccgtcgg cggcgagccc aggctggttg gcgatccacc gcgcgaacag
    12661 atcgttggtc cccatcgaca ccagcgagcg atcgacgccg gcgcggatcg ttcgcacgtc
    12721 gtggaagtcc tcgcgtccga tcagcgggag cacgctggtg agcacgagcc agagcagcaa
    12781 caacaggaac accggggttc gctgcacccg aaggaccttg aacaggcccg gtggttcgga
    12841 ccgttcgacg aacagagaca cccagccgac ggcggcggtc gacacggcga agaacgcgac
    12901 cacgagcgcg atcgcgccga acccctgccc aacggcccag gggttgacga gcacgcgcca
    12961 ccagagcagc acgccggcga cgatgagggc gtcgacctcg tagggatgac gacgggtgtc
    13021 cgtgggtgtc gccaccatga tggcgccgga cacggcccgg ccgacgatcg aacccggagg
    13081 cgacggtctg cccgaagccg gccggggctc gcgccgaacg gccgacgccc tggccgccgc
    13141 gtagaggaac ccgtagtaga agaaggcgat cgaggtcgcc gggacgacga tcgtccagga
    13201 cacgtacacg acggtgacgt acaggaacga gcccgcgtgg tcgtacatcg tgagcgggaa
    13261 cagcgatctc gcgacgaagt acgagagggc gatcggccag gctgcggcga gcagagccgg
    13321 cacctggtcc cggatcgggg cactcacctc gacgggagcg gccacgacga tgtcctcgcc
    13381 ggccgggtcg aacagaccga gggcgcccag gaacagcccg gggatcatca gaccgcgggg
    13441 acccaggtcg aaccagatcc ccgtgaaccc aaccaggaac agcgcgactc cggctccgaa
    13501 catccaccac cggacctgtc tgggatcgcg cccctggagg gggtggcccg cctcgtcctt
    13561 gccgcgccgc acgcgggtca cgcgccggcc gatgctccac gtcacgtacg cagccgccac
    13621 tgcggccagg aacgccaggg cgccgcgcgg caggtcccat ccacggaaga cgtccttggt
    13681 ctgggggttg gcccacataa gaacgaagaa gacgacgagc acgacgatcg ggcctcgcag
    13741 gcgtcgaagg gccgggcgca gcgagcggaa ccaatcggtg ccgagcctga cccaggcggc
    13801 cagagggaga agggcgacga cgatggcacc ggcgaccagg atcagcttgg tgtagctgac
    13861 cactcggagc agctggacga agccgtcgct cgggacgttc acgtcctcgc acaggccaga
    13921 tgggcaggcc tcgacgcgtc gatccacgaa ccgccagagc atgccgttct cgagcaggtc
    13981 gaagaacggg accaacgcga ggatcccagc cgcgatggcg gccgtcctct ggaaccccgt
    14041 cagcgtggac gacgccatgt cctcccgagc cttctgcttg accttctgct tcttcgtccc
    14101 ctggtcggtc tctctcacga gggtcgcccg ccgatgcgcc tcgtccttga cgaccttctg
    14161 ggcgcgccgg aacaggacgc cgaggaacag gacgtacgcg gggacgaacg cgaatgagtc
    14221 gatgcccagg aaccacgatg ccgccgtacg cggagccagc ggcggacgtg cgacggtgac
    14281 ggccgacgcg gtcgcgtacg gctgcgagaa ccagttctgc catgcgtcca ccgttgcccg
    14341 cgcgcggacg gggtccgcga tgatggccat cacgccgcgc ccggttcgac ccagcgggtg
    14401 catcgcctgc aagagcccct cgatctcggc catgacgccg agggtgatga acgcgacgat
    14461 cccggcgctg atgacgatca acgcccaccg atcggatgcc gctccgagcc ggcacagacg
    14521 ccaaagcgcg acggctcccc cgaccgcgac gaggacgacc ccgatcgcga tcacgatgga
    14581 catcggcccc tcccgcccgc cactcggcgc cttcgcggac gtacgccacc cgaggtcggt
    14641 cgtcaagtca gttctccggc gtacggcggt cgcaggtggt cggtgaccgt tgaccggtca
    14701 gccgatcacc ttgtggagac gccagcggcc ggggacgccc ttcaattggt gctcacccgc
    14761 ctcgtcgaac tgcagacccg aacccgggac gaggtccttc accgtctgtg agaccaggac
    14821 gtcgccggcc ccgccgatgg cggccacccg ggcaccgatc gagacggtga gaccgctgca
    14881 cttgtcctcg atcagctcac attcccccgt atgcacgcca gcccggatct cgatccccag
    14941 atcgcggacc cgttcggcca cctccagcgc gcagcggatc gcgcgcgcgg gaccgtcgaa
    15001 cgcggcgtag aagccgtcgc ccgcggtgtc gttctccctc ccgcgccacc gctcgagcgt
    15061 cgtccgcacg atcgagcggt actgcagcac gagttccttc cacgcgtggt cgccgaactc
    15121 cgcctgcttc tcgaccgacc cgacgatgtc ggtgaacagg accgtcgaca ggatgctgtc
    15181 gagttcggga ggcgggcgct ggatgccgca gaaatcgaac gtcgcctcga tgaccgccgg
    15241 cgcccagagc cgagacgccc tcgcgggcat cgacctgcag accgcgccgg ggatcagcgc
    15301 cgcggtcgcc cgggtctgct cgacgaccca ctcccccgga tacccctccg tccccagggc
    15361 caggaccggg atccggagcg tcgggaggat ctcgcgaacg tcgtagtcgt ggagcaatcg
    15421 gatgaacccg acggcggtcg caggcccaca cgaatgccgc tgcatcctcg cgaggaagcg
    15481 gatgacgtcg tcgtccatct cggcatgcgc gtacatgcgg cgcacgaagt cctccgtccc
    15541 ccaggcgcgc tccgcctccc gagcgaggcg atccacgtca tccgggtcgc gaccgaacga
    15601 ccacccgcgc gtcttcaccg atcgcgcctg cgcgaagaac cagacgagtc ctgcgacccg
    15661 atccgggtgc gtcgcggcaa ggagggcggc aaccatgccc ccatcggcgg acgccagcag
    15721 gatcggacga cgaagacccg ccgcgtcgag gaccgcgaga aggtcggccg cccgcgtctc
    15781 caagtcgggg aggccgccca tgtcatccga gagcccagtc cctcggcggt cgtggaggct
    15841 caaccgagag aaggacgcca gctccaacca agacctcgct gcgggctcga attcgaagaa
    15901 cagctcgacg ttggccgcga tggagccgat cacggcgacc tcgttctcgc tcgtgggatc
    15961 gccgagcagc ttgtacgcga tgtagccgcc gtccagcgtc ttcgcgtacc gggtctcggc
    16021 gacctcagtc acgcgtcccc ccatcgggtc gacgtgccag cggtcgggga agccatcggt
    16081 tcctgttcgt cgaggtcgaa ggctcagtgg gatatgagct caccgggccc tcccagttcc
    16141 cggatgatcc ccatcagtcg ctacccatac cacggacgga taacgccgtc ttcgccgtga
    16201 ctgggcgtcg agcagcctgc gagtccctgg cccctcgact ctcgcggagc cggggcatcg
    16261 cccggacgat cgctgtgaag caggccacgc gccccgtacc gtggacccgt gacggtggct
    16321 cggatcctcc atcgggaccg agaaggtcta cacgatcgtc tgcaccgacc cgacgtgagc
    16381 tcgatcgagg atccgtacga acgcctcgtt ggccttccgg cggatctcct cttcatcgac
    16441 cgtcaggagc tcccgttccc gcatcaccac cgcaccgtcg atgacgacgg aactcaccgc
    16501 gcgcccatgt gaagcgaagg cgagatggga atagagctgg ttgcgattgc ctgggacgag
    16561 aggggtgaag cccacgtcgt cgagctcgat cacgatgacg tcggccttct tcccctcggc
    16621 gagcatgccg gtgtcgtgcc gcagcgcgtc cgaaccgttg cgggtggcca tgcggagcag
    16681 atccccggcc tgcaggacgt tggcgtccat tcggacggcc cgctgcatca gcgacgcgaa
    16741 cttcatgacc tcgaacatgt cacccgtgtt gacaccctcg gtcgagtcgt gtccgagccc
    16801 gacgttgatc ccggcccgca ggtagtcgag aagacgagcc accccgtttc ccaacttggc
    16861 gttcgacgtc gggttgtgcg agatgtgggt gccggtatcc cgcatcaacc cgatctccgt
    16921 gtccgacagc cacacgcaat gagcggcgac ggtgctcgag cccaggatcc cgcactcgta
    16981 cgcgacttcc gtggggcggc gcccgaactt ctccttcgag atctcgacct cgccgacgga
    17041 ctcgttgaga tggatgtgga tccccgtacc cagatcgcgt gccagcgcgc tcgcgtctcg
    17101 caggaggccg atcgaagcca gcggaagcca ctcgatgccg accttgacct cgatccgtcc
    17161 gttggcggac ccgtctctcg
//
LOCUS       DATBRK010000112         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2615490, whole genome shotgun sequence.
ACCESSION   DATBRK010000112 DATBRK010000000
VERSION     DATBRK010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615490"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VIH70_03405"
     CDS             <1..447
                     /locus_tag="VIH70_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8115440.1"
                     /translation="VMLGNHQAAQDLVQDTFMKLWRTADRYDPQRGRLETWVLLTARS
                     LAIDALRRRVLESRSLRSVGVQQEEDPQPLPDEQAMVADLSARARRAMGVLSPEQRSA
                     LELAYFGGRTTAEVAALEGIPQGTVKTRIRSALLRLRAEMAKGSAR"
     gene            444..1193
                     /locus_tag="VIH70_03410"
     CDS             444..1193
                     /locus_tag="VIH70_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8115441.1"
                     /translation="MTCDEVRELLPEHLLGTLQGPEDLEVRRHLRGCAACREERVKLE
                     EGVSALSRAVHDRQPPPELRDRVLGTLGEEWEESVPLPATPVPSRGAGRERSPWRAFA
                     VAAALILVVGSIAFGFAQGHRASLATTDAQSYRNLLRSLGGKEFRIGELRPAEGSSMH
                     GQVLIYDGDPSGDWSSWALVFAKVPGYSGSATATLLSPEGDSRALRLIEFQEGDGETW
                     LVTYADLTRFDRLTVTSPTGQVLATATLEPA"
     gene            1508..1855
                     /locus_tag="VIH70_03415"
     CDS             1508..1855
                     /locus_tag="VIH70_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115442.1"
                     /translation="MDPAKVEFLFGRVPTWADTDDRDDRSVLLREQRLGRRDDDVGRF
                     GLAMYEAIADQIANGEPPEVWETARCDEPVPPLSGMTPRQAAADPTRRETLERLLASF
                     EDRSAPLTGAAWR"
     gene            complement(1957..2481)
                     /locus_tag="VIH70_03420"
     CDS             complement(1957..2481)
                     /locus_tag="VIH70_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY8115443.1"
                     /translation="MARLSGHRVGVFVEEGFEDLEFWVTVMRLREEGADVTIVGTHAG
                     ERYRGKNGLSAASDVAAKEVSADDFDAVVVPGGWAPDKLRRHDEVTGLVRKAYEQGKI
                     VGLICHAGLVGISAGIVEGHRATGSLGIRDDLVNAGATWVDEPAFRDGTIVWGRVVED
                     IPDFCRELVAAIAP"
BASE COUNT          397 a         1005 c          950 g          428 t
ORIGIN      
        1 gtgatgctcg gcaaccatca ggccgcacag gacctcgtac aggacacgtt catgaagctc
       61 tggagaaccg ccgatcgcta cgacccgcag cgcgggcgtc tggagacatg ggtgctgctg
      121 accgcgcggt cgctcgcgat cgacgccctg cgtcggcgcg tcctggaatc acggagcctc
      181 cggtcggtgg gcgttcagca agaggaggac ccccagcctc tcccggacga gcaggcgatg
      241 gttgccgacc tgtcggcccg tgcgcgacgg gcgatggggg tcctctctcc cgagcaacgc
      301 tccgcgctgg agctcgccta cttcggtggc cggaccacgg ccgaggtggc agcgctcgag
      361 gggatcccgc aagggaccgt gaagacccgg atccgatcgg ccctgttgcg tctgcgggcc
      421 gagatggcaa aggggtccgc gcgatgacgt gtgacgaggt ccgcgagctc ttgcccgagc
      481 acctgcttgg cacgctccag gggcccgagg acctcgaggt ccggcggcac ctccgtgggt
      541 gcgccgcctg ccgtgaggag cgggtgaagc tcgaagaggg cgtctcggcc ctgtcccgtg
      601 ccgtccacga tcgacagccg ccgcccgagc ttcgcgaccg cgtcctgggc acgctcggcg
      661 aggagtggga ggagagcgtg cctttgccgg cgacgcccgt cccctcccgc ggggccggcc
      721 gcgagcgctc gccctggcga gccttcgccg tcgccgccgc gctgatcctg gtggtcggct
      781 cgatcgcctt cggcttcgcc caggggcacc gcgcctcctt ggccacgacc gacgcgcaga
      841 gctaccggaa cctgctgagg tcactcggtg ggaaggagtt caggatcggt gagctccgac
      901 cagccgaggg atcctcgatg cacgggcagg tgttgatcta cgacggcgac ccgtccggcg
      961 attggtcctc gtgggcgctc gtgttcgcga aggtgcccgg ctactcgggt tccgcgacgg
     1021 cgacgttgct ctcgcccgag ggcgattcgc gggctcttcg actgatcgag ttccaggagg
     1081 gcgacgggga gacctggttg gtcacctacg ccgacctgac tcgattcgac agactcacgg
     1141 tcacctcccc gaccgggcag gtgttggcga ccgcgacgct cgaacccgcc tgaactcgga
     1201 cgtctcccgc ttcctcttca ggaatcggag caggcgttgg agtgccggta tcggatctcg
     1261 gtgccgtccg gggcatgacc gactcgctct cgacgaacgg cctcacgagg cgtcgtaatc
     1321 ggaggcatcc ggccaccgcc gttgtgaggg cgtcgtggac gaccacacgg cagctggggc
     1381 gcaactgctg gacgtgcccg actcgcttcg aacgggtact taagggcaag ggcccaggcg
     1441 agggctgacc cctgtccgcc ggcctcggtc cgtttgtccg tcttgccggc ctccgtaccc
     1501 tggtgccgtg gatcccgcga aagtcgagtt cctcttcggt cgagtgccga cgtgggccga
     1561 caccgacgat cgcgacgatc gctcggtcct gctgcgggag cagcgactcg gtcggcgcga
     1621 cgacgatgtc gggcggttcg ggctcgccat gtacgaggcg atcgcggacc agatcgcgaa
     1681 cggcgagccg cccgaggtgt gggagacggc ccggtgtgat gagccggtcc ccccgctctc
     1741 tggcatgacc ccgcgccagg ccgccgccga cccgacgcgt cgcgagacgc tcgagcgcct
     1801 cctggcgagc ttcgaggatc ggagcgcccc gcttaccggg gcggcgtggc gatgacgatg
     1861 agaccggccc cgccttcggc tcttcctcgg cctggccgac gacccttggt agcggccacg
     1921 ggctgaaggc atcgagtctc gcctgcctcg cctcggctag ggagcgatgg cggcgacgag
     1981 ctccctgcag aagtcgggga tgtcctccac cacacggccc cagacgatgg tcccgtcgcg
     2041 gaaggccggc tcgtccaccc aggtggcgcc ggcgttgacg aggtcgtccc tgatcccgag
     2101 gcttccggtc gctcggtgac cctcgacgat ccctgccgag atcccgacca ggccggcgtg
     2161 gcagatcaac cctacgatct tgccctgctc gtaggccttg cgaacgaggc cggtgacctc
     2221 gtcgtgcctc cgcagcttgt ccggcgccca gccgccgggg accacgaccg cgtcgaagtc
     2281 gtcggccgag acctccttgg ccgccacgtc gctcgccgcc gaaagcccgt tcttgccgcg
     2341 gtaccgctcg cccgcgtgcg ttccgacgat cgtgacgtcg gcgccctcct cgcgcaggcg
     2401 catgacggtc acccagaact cgaggtcctc gaacccctcc tctacgaaca ccccgaccct
     2461 gtgccccgaa agccgtgcca tcgcatgcct cctcggttcg tcgacgccaa cgaaggctac
     2521 tggtcgaccg catgccggcg acgctgggct cggccagccg ccccaggatt ccccggcgat
     2581 ccccgcaccg aacggtatcg agctgcgcgc ggcccggaaa atgtccgatc cgtcagcggc
     2641 cccgctcgta gacgcgcgcc cccgccatgt cccgcgacag gccgcgggcc acctgcttga
     2701 cggcgccggc cagatcgggc cgcgcgaagc cgtccaccag aagccgttcg accggaccga
     2761 cgaccccaat ggcgcccacg
//
LOCUS       DATBRK010000113         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2615810, whole genome shotgun sequence.
ACCESSION   DATBRK010000113 DATBRK010000000
VERSION     DATBRK010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615810"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VIH70_03425"
     CDS             <1..336
                     /locus_tag="VIH70_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115444.1"
                     /translation="GRLFVSMMWRSRKALDLLRDPRIAVHSVTTNKDGTDGDVKIYGH
                     SADERDPDVRAAFREAIRRRIDWAPDEPEYHCFSVDVRSAGYTRFGGKAEAVAWDEER
                     GLRRLPPPG"
     gene            complement(338..>1730)
                     /locus_tag="VIH70_03430"
     CDS             complement(338..>1730)
                     /locus_tag="VIH70_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8115445.1"
                     /translation="IQNLLDFERDYPDAEVFLMEQNYRSTSNILAIANALIEHNVQRK
                     PKSLWTETTEGELAVRFRADDEHEEALFVAEETHRLVEDEAHRYADVAVFYRTNAQSR
                     VLEDIFMRAGIPYRVVGGVRFYQRKEIKDVLAYLRLLLNPQDVISARRVVNTPKRGIG
                     DATVAALEGFAAIEQVAFLEATRRADELPELGARAKGAVAGFVGVMLRLQEAYDSGAG
                     PQRMVEAAATESGYLLELEAERTVEAEGRIENIRELGGVAAEFEQRSPDGSLAEFLEQ
                     VSLVSEQDEYDEESGSVTLMTLHNAKGLEFPVVFIIGLEDGVFPHYRSMGDPAQLEEE
                     RRLMYVGVTRARERLYLTFAWSRTLFGSTSYSPPSRFLGEIPSELVRALEEGDLVVAS
                     DSSVSPIRAAVEGVREIPQISAGDTVRHDKWGEGVVLTVSGAGSDAEATVRFEEAGEK
                     RLLVAYAPLRKVG"
BASE COUNT          255 a          625 c          586 g          264 t
ORIGIN      
        1 ggccggctgt tcgtctcgat gatgtggcgg tcgaggaagg cgctcgacct gctccgcgat
       61 cctcggatcg cggtccacag cgtgacgacg aacaaggacg gtaccgacgg cgacgtgaag
      121 atctacggcc actccgccga cgaacgggac cccgacgtcc gcgccgcctt ccgcgaggcg
      181 atccggcggc ggatcgactg ggcaccggac gaacccgagt accactgctt ctcggtggac
      241 gtgcgctcgg cgggatacac tcggttcggc ggcaaggcgg aggcggtggc gtgggacgag
      301 gaacgcgggc tgcggcgcct ccccccgccc ggttagctca gccgaccttc cgcagtggcg
      361 cgtaggcgac caggagccgc ttctcccccg cctcctcgaa ccgaaccgtc gcctcggcgt
      421 ccgaaccggc gccgctcacg gtgaggacga cgccctcgcc ccacttgtcg tgccggaccg
      481 tgtcgcccgc cgagatctgg gggatctcgc gcacgccctc gaccgccgcg cggatgggtg
      541 acaccgagga gtccgaggcc acgaccagat cgccctcctc gagggcccgg acgagctcgc
      601 tcgggatctc accgaggaac cgcgacggcg ggctgtacga cgtcgagccg aagagcgtcc
      661 tgctccaggc gaacgtcagg tacagacgct cgcgggcgcg cgtcaccccc acgtacatca
      721 ggcgccgctc ctcctcgagc tgggccggat cgcccatgga gcggtagtgc gggaacacgc
      781 cgtcctccag gccgatgatg aagaccactg ggaactcgag gcccttggcg ttgtgcagcg
      841 tcatgagcgt cacgctcccc gactcctcgt cgtactcgtc ctgctcggag acgagcgaca
      901 cctgctcgag gaactcggcg aggctcccgt ccggcgagcg ctgctcgaac tcggccgcca
      961 cgcccccgag ctcgcggatg ttctcgatcc ggccctccgc ctcgacggtg cgttcggcct
     1021 ccagctcgag gaggtacccg gactcggtgg ccgcggcctc caccatccgc tggggccccg
     1081 cgccggagtc gtacgcctcc tggaggcgaa gcatcacccc gacgaacccg gcgacggcgc
     1141 ccttggcccg ggccccgagc tcggggagct cgtcggcccg gcgcgtggcc tccaggaagg
     1201 cgacttgctc gatcgccgcg aagccctcga gcgcggccac cgtcgcgtcc ccgatgccgc
     1261 gcttgggtgt gttcaccacc cggcgtgcgc tgatcacgtc ctgcgggttc agcagcaacc
     1321 gcaggtaggc gagcacgtcc ttgatctcct tgcgctggta gaaccgcacc ccaccgacga
     1381 cgcggtaggg gatgcccgcc cgcatgaaga tgtcctccag aacccggctc tgggcgttgg
     1441 tccggtagaa gaccgccacg tccgcgtacc ggtgagcctc gtcttcgacg agccgatggg
     1501 tctcctcggc gacgaacagc gcctcctcgt gctcgtcgtc ggcgcggaac cgcaccgcga
     1561 gctcgccctc ggtcgtctcg gtccacaggg acttgggctt gcgctgcacg ttgtgctcga
     1621 tcagcgcgtt ggcgatcgcg aggatgttcg aggtggagcg gtagttctgc tccatcagga
     1681 agacctcggc gtcggggtag tcgcgctcga agtccaggag gttctggatc
//
LOCUS       DATBRK010000114         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1025496, whole genome shotgun sequence.
ACCESSION   DATBRK010000114 DATBRK010000000
VERSION     DATBRK010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025496"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VIH70_03435"
     CDS             complement(<1..195)
                     /locus_tag="VIH70_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HEY8115446.1"
                     /translation="MDPLRPTGQDVVGIVFRVPLVDGGDQLAYILHRGDTKDPGPDQF
                     LVSANYGHEVWRLQGRRPGEA"
     gene            complement(274..930)
                     /locus_tag="VIH70_03440"
     CDS             complement(274..930)
                     /locus_tag="VIH70_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEY8115447.1"
                     /translation="MLVPARPTRRGLYLRAVLDVLALGALVYLVKSPLVWLLMAINVA
                     LYFVGAAIARRMGQRLFRPLLVAWLLAFPVVVVLLSFGDVSTDQWGGLMLNVIVVVPV
                     ISLSFPLGILLALGRRSSLPAISWFCVGFIELLSAASRGDSSSGDTAGVPNVVGMDLQ
                     SAQDTLQANGFYNLGSHDVTGEASFQILDRNWEVVDQTPSAGTMTDMSVRVELGVVRK
                     "
     gene            1045..1437
                     /locus_tag="VIH70_03445"
     CDS             1045..1437
                     /locus_tag="VIH70_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115448.1"
                     /translation="MDLLGTSINAAVVAVVGALLAWLARGRFDALDRRIDRLETRLVG
                     TETRLDGRIDEMGARLEGRFDRMESRMDGIEPRLGSRMDALGARFDGLESRFDGRFDA
                     LQSSMDAMRSDLTQVALAVGVRPRAQND"
     gene            complement(1504..1782)
                     /locus_tag="VIH70_03450"
     CDS             complement(1504..1782)
                     /locus_tag="VIH70_03450"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEY8115449.1"
                     /translation="MEGEALRLDGNAIAGTLGEVFVHEMTAARIACKGCGNVEPVGAE
                     HAYVQAPGIVLRCSHCDSALLVMTRTAGRHRIWFPRSHWLETGEAGNS"
BASE COUNT          321 a          602 c          599 g          294 t
ORIGIN      
        1 ggcttctccg ggtcggcgcc cctgcagccg ccacacctcg tgaccgtagt tcgcggacac
       61 caggaactgg tcgggtccgg gatccttcgt gtcgcctcgg tgcaggatgt acgcgagctg
      121 gtcgccgccg tcgacgagcg gcacccggaa cacgatgccg accacgtctt gaccggtcgg
      181 gcggagcggg tccacgccgc tgcacgaccg ccccggtcca cacttgcagt ccgcaaaggt
      241 cgttgaagtc cgtgctggtc gggtcccccg tacctacttg cgaactactc caagctccac
      301 acgaaccgac atgtcggtca tcgtccccgc ggaaggagtc tggtccacca cctcccagtt
      361 tcggtccaga atctggaagc ttgcttcgcc tgtaacatca tgtgacccta ggttgtagaa
      421 accgttcgct tggagcgtgt cttgtgcgct ctggaggtcc atcccgacca cattcgggac
      481 cccagcggta tcgccagaac tactgtctcc gcgggacgcc gccgataaga gctcgatgaa
      541 cccgacgcag aaccacgaga tcgcgggcag tgagctccga cggcccagcg ccagcaggat
      601 cccgagcggg aacgacagcg agatcaccgg gaccacaacg atcacgttga gcatcagccc
      661 gccccactga tccgtggaga cgtcgccgaa cgacagcagc acgaccacga cggggaaggc
      721 aagcaaccag gccacgagca ggggccggaa gagccgctga cccatcctcc gggcgatggc
      781 cgcgccgacg aagtagagcg cgacgttgat cgccatgagc agccacacga gcgggctctt
      841 caccaggtac acgagggcgc cgagcgcgag cacgtcgagg accgcccgca ggtagaggcc
      901 tcggcgcgtg ggacgcgccg gcacgagcat cgcgtgcagt atcgcggtcg actcggatgc
      961 agggccggcg acgactccgt cggagccgcg tgctagggtc gataggtctt ggtggtgcgc
     1021 ctctccaggg agaatgcgag gctgatggat ctcctgggaa cgtcgatcaa cgcggcggtc
     1081 gtcgcagttg tcggagccct tctggcatgg ctcgcgaggg gtcgcttcga cgcactcgac
     1141 cggaggatcg accggctgga gacgcggctc gtcgggacgg agacgcggct cgatggacgg
     1201 atcgacgaga tgggtgctcg gctcgagggg cggttcgacc ggatggaatc ccggatggac
     1261 gggatcgaac ctcgccttgg atccaggatg gacgcgctgg gagctcgatt cgacgggctt
     1321 gaatctcgat tcgacgggcg gttcgacgcc ctccagtcgt cgatggacgc gatgcgttcg
     1381 gatctcacgc aggtggccct cgccgtcggc gttcgtcctc gggctcagaa cgactgatcg
     1441 gcgcgacata gagattcagt gtcggccccg agggggatta agggccgagc ttcgaggctg
     1501 gactcaggag ttccccgcct cgcccgtctc cagccagtgc gagcggggga accagatacg
     1561 gtgccgtccc gctgtccgag tcatcaccaa cagggccgag tcgcagtggc tgcatcgcaa
     1621 cacgatgccg ggggcctgca catacgcgtg ctccgctccg acgggctcca cgttgccaca
     1681 tcctttgcac gcgatccgag ccgccgtcat ctcgtggacg aacacctcgc ccagcgtgcc
     1741 cgcgatcgca ttgccgtcga gcctgagcgc ctcgccttcc atctagcctc ccgtcggtcc
     1801 gaatcgctcg gtcttc
//
LOCUS       DATBRK010000115         5032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1605042, whole genome shotgun sequence.
ACCESSION   DATBRK010000115 DATBRK010000000
VERSION     DATBRK010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605042"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VIH70_03455"
     CDS             complement(<1..201)
                     /locus_tag="VIH70_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115450.1"
                     /translation="MTAPRSAVRRLAIARVISVTGSAAAYTALMFEIYDQTGSSVWLA
                     AALIVTEGVTGLVGPLASVLGDR"
     gene            complement(198..1394)
                     /locus_tag="VIH70_03460"
     CDS             complement(198..1394)
                     /locus_tag="VIH70_03460"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8115451.1"
                     /translation="MTAPRTAVRRLATARIISITGGAAAYTALNFTILEQTGSAAWVA
                     VSLLLTFGVTGFVVPPAGILGDRFDRRKVLIVSDLAGAACFAMMALSSEPAWLLPWGF
                     LSAVAEAPFWSASDSAIPNVAGPELTTWANSLLGASRLAGVAIGPALGGALLATTSPS
                     IVFGLNAVSFVLSALIIVTVHGRFSSEPSEEEFRGMRAGFRFLRHDRVLRTLLLAWIV
                     FISGMGMSMVADAPLAEVFEAGPFGFGLLITAWGGGSALGALFARRMQTGSEGTWLVS
                     AGVVIAATGIGIGLSPWFALAVTLMFVMGLADGTTIVAETNLRQRRPPDAVRSRVTAA
                     FSGVMHLMLAFSYIVAAFVVPWIGPKPTYVVGGVTAGLAVFVLLRTKRYLELEEAARL
                     EGGEHR"
     gene            complement(1427..2701)
                     /locus_tag="VIH70_03465"
     CDS             complement(1427..2701)
                     /locus_tag="VIH70_03465"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8115452.1"
                     /translation="MRSHSQPRSAVRRLALARLISIAGGAAAFAALNFTVYERTGSAT
                     WLSASLLLTFGVSGVFAPLGGMLGDRFDRKRVMIVSDLAGAACFLALAFVHEPALLLA
                     LGFVAAVVEAPFWSASAAAVPNLVGKDDLAWANGLLQVGGNAGIMLGPALGGVLLAAI
                     GSGMVFGLNAFSFVVSAAIIATVRGRFAEDRTEHDDAHRGFRAGFVFIARDRVLRTIV
                     LAWVALVFGIGLAMVADVPLAELFGSGSTGYGLMIGAFGAGAVLGSLAGRKLHERNEA
                     RALIVGTIVMGATTAGIALSPWFPPILALLVLCGAADAVIMIADRSIQQRRTPDAVRS
                     RVVGASEAMIDIALSIGFVLGGPAVAALGPRAVYAIGGACGLLGAVVLVPVLRASRRA
                     SPAAAATDPETEDEDVQEHAEADERVVARVAS"
     gene            complement(2934..4517)
                     /gene="serA"
                     /locus_tag="VIH70_03470"
     CDS             complement(2934..4517)
                     /gene="serA"
                     /locus_tag="VIH70_03470"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY8115453.1"
                     /translation="MRVLVTEKLSDAGLDQLRKDFEVDVRPELAADGLAEAIAPYDAL
                     VVRSQTQVTSDVIEAGDNLKVVARAGIGLDNVDVDAATRRGVMVVNAPSSNIVSAAEH
                     TIALLLAQARNIPQAHDDLKRGHWDRSKWQGVELQGKTLGIVGLGRVGALVAQRSVAF
                     GMRVIAYDPYVPRARAKEMGIELMPSLEALLVQSDFVTIHLPRSPETEGLIGERELAL
                     VKEGARLVNTSRGGIVDEQALAKALEDGRVAGAALDVFEHEPPEDSPLFGFEQVVTTP
                     HLGASTAEAQDKAGTSVAEMVRLALNGEFVPYAVNVSAAAEVAEIVRPFLPLAERLGA
                     LLTGLAEGAVRTVQAQYLGRIGEADTRVLTLAVVKGVLQGVVHEPVSFVNAPIIARER
                     GIAISETRSSVSSDYVNLVAVTAETDQGEVSVAGTLVGKRNDQRVMHVNGYDIEMAPA
                     TNMLFFEYEDRPGVIGKVGTILGEHQVNIATMDVGRASRGGTALMGLTLDSPAGQDVI
                     DEITSAIGGRAARSIVLPD"
     gene            complement(4597..>5032)
                     /locus_tag="VIH70_03475"
     CDS             complement(4597..>5032)
                     /locus_tag="VIH70_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEY8115454.1"
                     /translation="AISVMQGLHAALELYFQDGVDVALERHRTLSRAVKDGAQALGLD
                     LFGEGLDDNWTVTAIRAPEAADADTISDRMRSDFGCVIAPGQGPLKGTVFRIGHFGYF
                     SELDIVRGLAALEMTLERLGHPVKRGAAVAAAEGVFLGGPGG"
BASE COUNT          814 a         1875 c         1687 g          656 t
ORIGIN      
        1 ccggtccccc agcacgctgg cgagcggacc gaccagcccg gtcacgccct cggtgacgat
       61 cagcgccgcc gcgagccaga ccgaagaccc cgtctggtcg tagatctcga acatcagcgc
      121 cgtgtacgcg gccgcgctcc cggtgaccga gatcacccgc gcgatcgcca gccggcggac
      181 cgcggatcgc ggtgcggtca tcggtgctcg ccgccttcga gccgtgcggc ctcttcgagc
      241 tccaggtacc gcttcgtccg cagcaggacg aacaccgcca acccggcggt gaccccgccc
      301 accacgtagg tgggcttcgg cccgatccac ggaacgacga acgcagccac gatgtacgag
      361 aacgccagca tgaggtgcat cacgccgctg aacgcggccg tcacacggct tcgaacggcg
      421 tcgggtggtc gtcgctgccg caggttcgtc tccgccacga tcgtcgtccc gtcggccaga
      481 cccatgacga acatcagtgt caccgccagc gcgaaccacg gcgagaggcc gatgccgatc
      541 ccggtggccg cgatcacgac gcccgccgat accagccacg tcccctcgct ccccgtctgc
      601 atgcgcctcg cgaacaacgc gcccagcgcc gaacccccgc cccatgccgt gatcaaaagc
      661 ccgaagccga acggtccggc ctcgaagacc tccgcgagcg gagcgtctgc gaccatcgac
      721 atccccatgc cggagatgaa cacgatccac gcgagcagga gcgtccgaag cacccgatcg
      781 tgtcgaagga agcggaaccc cgcgcgcatg cctcggaatt cctcctcgga gggttcgctc
      841 gagaagcgcc cgtggaccgt cacgatgatc agggccgaca acacgaacga gacggcgttg
      901 aggccgaaca cgatgctcgg gctcgtcgtc gcgaggagcg cgccgcccag tgccgggccg
      961 atcgcgactc cagccaatcg gctcgcgccg agcaggctgt tggcccacgt ggtgagctcg
     1021 gggccggcga cgttggggat cgcggagtcc gaggcggacc agaatggcgc ctcggccacc
     1081 gccgacagga agccccacgg cagcagccac gcgggctcgg aggacagcgc catcatcgcg
     1141 aagcacgccg cgccggccag gtcggacacg atcagcacct tgcgccggtc gaatcggtcg
     1201 cccaggatcc cggccggcgg cactacgaac cccgtgacgc cgaacgtcag cagcagggag
     1261 accgccaccc acgccgccga gcccgtctgc tccaggatgg tgaagttcag cgccgtgtac
     1321 gccgcggccc caccggtgat cgagatgatc cgggccgtgg cgagccgccg gacggcggtt
     1381 cggggggcgg tcaccgaggg agtgtccacc gccccccgac ctccgtctag ctcgcgaccc
     1441 gagcgacgac gcgctcgtcc gcctcggcgt gttcctgcac gtcctcgtcc tcggtctcgg
     1501 gatcggtcgc ggccgcagcc gggctcgccc gccgagatgc gcgcagcacg ggcaccagca
     1561 ccaccgcacc cagcagcccg cacgccccac cgatcgcgta cacggcacgc gggcccagcg
     1621 cggcgaccgc gggaccaccg aggacgaacc cgatcgacaa tgcgatgtcg atcatcgcct
     1681 ccgacgcgcc caccacgcgg ctgcgcacgg cgtcgggcgt ccggcgctgc tggatgctcc
     1741 ggtccgcgat catgatcacc gcgtccgcag cgccgcagag cacgagcagc gcgaggatcg
     1801 gcgggaacca cggcgagagc gcgatgcccg ccgtcgtggc gcccatcacg atcgtgccga
     1861 cgatcagcgc gcgcgcctcg ttgcgttcgt gcagcttccg acccgcgagc gacccgagca
     1921 cggccccggc gccgaacgcg ccgatcatca gcccgtagcc ggtcgagccc gacccgaaca
     1981 actctgcgag cgggacgtcg gccaccatcg ccagcccgat cccgaagacg agcgccaccc
     2041 acgcgaggac gatcgtgcgc agcacgcggt cgcgcgcgat gaacacgaac cccgcgcgga
     2101 acccgcggtg cgcatcgtcg tgctcggtcc gatcctccgc gaaccgtccg cggacggtcg
     2161 cgatgatcgc cgccgacacg acgaacgaga aggcgttcag gccgaacacc atccccgacc
     2221 cgatcgccgc gagcagcacg ccgcccaggg cgggtccgag catgatcccc gcgttcccgc
     2281 cgacctggag caggccgttc gcccaggcca ggtcgtcctt gccgaccagg ttcgggacgg
     2341 ccgcggcaga ggccgaccag aacggcgcct ccacgaccgc ggccacgaat cccaacgcga
     2401 gaagcagcgc cggctcgtgc acaaaggcca gcgcaaggaa gcacgcggcg cccgcgagat
     2461 cggacacgat catcacccgc ttgcggtcga agcgatcccc cagcatcccg ccgagcggcg
     2521 cgaacacacc ggatacgccg aacgtgagca gcagcgacgc cgagagccac gtcgccgagc
     2581 cggtccgctc gtagacggtg aagttcagcg cggcgaaggc cgccgcgccg cccgcgatcg
     2641 agatcaggcg tgcgagcgcg agccttcgaa cggcgctacg aggttgtgag tgagagcgca
     2701 tcggacgact cctgtgactc ggttggatcg agggacggtg gttcgggcgg tcccgccgga
     2761 gaggcggggg cgcgaccgct agacggccgc gatgaaggac gggatgagac ccgtcctcct
     2821 gctcccgaac gacacctgga gtcgcctcat ggtcgtgcga gtgtagcagc gcggaccttg
     2881 cgaaggaagc accctgtgac gaacgtcaca ggtgcttcgc aacgttggga cgatcaatcc
     2941 gggaggacga tggaccgcgc ggcgcgaccg ccgatcgccg aggtgatctc gtcgatcacg
     3001 tcctggcccg cggggctgtc gagcgtgaga cccatcaaag ccgtgccgcc gcgtgaggct
     3061 cggccgacgt ccatcgtcgc gatgttgacc tggtgctcgc ccaggatcgt tccgaccttg
     3121 ccgatcacgc ccgggcggtc ctcgtactcg aagaacagca tgttggtggc gggcgccatc
     3181 tcgatgtcgt agccgttcac gtgcatcacg cgctggtcgt tccgcttgcc gaccagcgtg
     3241 ccggcgaccg agacctcgcc ctggtcggtc tcggcggtca ccgcaacgag gttcacgtag
     3301 tcgctcgaga ccgacgagcg cgtctcgctg atcgcgatcc cgcgttcgcg cgcgatgatc
     3361 ggggcgttca cgaacgacac gggctcgtgg accacgccct ggagcacgcc cttcaccacc
     3421 gcgagcgtga gcacgcgggt gtccgcctcc ccgatcctgc cgaggtactg ggcctgcacg
     3481 gtccggacgg cgccctcggc cagaccggtg aggagcgcgc cgaggcgttc ggcgagcggg
     3541 aggaaggggc gcacgatctc ggcgacctcc gccgcggccg agacgttcac agcgtacggg
     3601 acgaactcgc cgttcaaagc gaggcgcacc atctcggcca ccgaggtgcc ggccttgtcc
     3661 tgcgcctcgg cggtggaggc ccccagatgc ggggtcgtca ccacctgctc gaacccgaac
     3721 agcggcgagt cctccggagg ctcgtgctcg aacacgtcca gcgccgcgcc ggcgacccgg
     3781 ccgtcctcga gcgccttcgc gagcgcctgc tcgtcgacga tgccaccgcg ggacgtgttc
     3841 acgagtcgcg cgccctcctt caccagcgcg agctcgcgct ccccgatcag gccctcggtc
     3901 tccggcgagc ggggcaggtg gatcgtcacg aagtccgact gtacgagcag cgcctcgagg
     3961 ctcggcatca gctcgatgcc catctccttc gcgcgcgcgc gcggcacgta ggggtcgtag
     4021 gcgatcacgc gcatcccgaa cgcgacgctc cgctgcgcga cgagcgcgcc gacgcgcccg
     4081 agccccacga tcccgagcgt cttgccctgg agctcgacgc cctgccactt cgagcggtcc
     4141 cagtggccgc gcttcagatc gtcgtgcgcc tgcgggatgt tccgggcctg tgcgagcagc
     4201 agcgcgatcg tgtgttcggc cgccgagacg atgttcgacg acggcgcgtt caccaccatc
     4261 acgcctcgtc gggtcgcggc gtcgacgtcc acgttgtcga gcccgatccc ggcacgggcg
     4321 acgaccttga ggttgtcgcc cgcctcgatc acgtccgagg tgacctgggt ctgcgaccgc
     4381 acgacgagcg cgtcgtacgg ggcgatcgcc tcggccaggc cgtcggccgc gagctcgggt
     4441 cgcacgtcga cctcgaagtc cttgcggagt tgatcgaggc ccgcgtccga cagcttctcg
     4501 gtgacaagca ctctcatggc ggcaatcgtc cttcggtcag gcgccgaacg cgtcttgcca
     4561 ccgggcggct accgctgcgc tctcgcgctc gctcaatcag ccccccggac cccccaggaa
     4621 gacgccctcg gccgcggcga cggccgcgcc gcgcttgacc gggtggccga gccgctcgag
     4681 tgtcatctcg agcgcggcca ggccacggac gatgtcgagc tcgctgaagt agccgaagtg
     4741 cccgatccgg aacaccgtgc ccttgagcgg gccctgcccc ggcgcgatga cgcagccgaa
     4801 gtccgaccgc atccggtcgc tgatcgtgtc ggcgtcggcg gcctcgggag cgcggatcgc
     4861 tgtgaccgtc cagttgtcgt ccaggccctc cccgaacagg tcgagcccga gcgcctgcgc
     4921 gccgtccttg accgcgcgcg agagcgtgcg gtggcgttcg agcgcgacgt cgacgccgtc
     4981 ctggaagtac agctcgagcg cggcgtgcag gccctgcatc acgctgatcg cg
//
LOCUS       DATBRK010000116         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1605180, whole genome shotgun sequence.
ACCESSION   DATBRK010000116 DATBRK010000000
VERSION     DATBRK010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605180"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VIH70_03480"
     CDS             complement(<1..1496)
                     /locus_tag="VIH70_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8115455.1"
                     /translation="MSATQGLHGPIDERLLDVRTPAELAFSPDGSRVAFALHSTVAEV
                     GSHPPSDLYLVERDSDEPVQVTTGVWSDRSPAWSPDGSRLAFLSDRITPGHQLPYTMA
                     VGAEPVLAANLHGSAEAVSWSRSRDLLLVLAADPGCYGLDWSARAVRGAEPAPDPTII
                     KPGDARRRLFEIHLASGDVTEVGPPGMSVWEFDGAPDDILVALVSEDPSTAGWYRSVV
                     ARLDLAERTAQTLYRPTWSLEGLALSPDGRRAAVVEGYSSDPGLLSGSVMIVDLADGT
                     TTDPWPGLQTVGLAEWIDDESLWYARCDGTGTACGRISLDGAVEERWRGDAFIGDEVT
                     KPRCTIAGGGSVVWTTHQAHGRPPELARFDHDTGEWTRFTSFNDDVIADTVFPEVRTI
                     RWTTPDGIEIEGLLMTPRGAKAPLPLLTLVHGGPTWNWGAFFSDSEPNAVLLASAGYA
                     CLLPNPRGSIGRGHAFAQAVIGDSGGMDFQDVMAGVDHCIAAGVADRER"
     gene            1584..2228
                     /locus_tag="VIH70_03485"
     CDS             1584..2228
                     /locus_tag="VIH70_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115456.1"
                     /translation="MHALRGGRTIALVVIVAALVACGGAPEVQDPKPAPEPASGGIPW
                     PAPSHPLDLTVAAGLEPTTHEFLDFHVHAHLDVFVNGSPLEVPAGIGINIEDPAVHIV
                     EDDLGTTYGGIDPPCAQPCISPLHTHGTDGILHTESAVDHPNTLGQFFTEWGVELTDT
                     CVGGYCSPGASIQVFVNGEPYEGDPAQIQLIDRLEIAIVIGSPPEEVPSTFPEA"
     gene            complement(2316..>3144)
                     /locus_tag="VIH70_03490"
     CDS             complement(2316..>3144)
                     /locus_tag="VIH70_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115457.1"
                     /translation="DPKRRFASAAELEAALLAVPLARGGDTMRLETVVAAETQPVAPV
                     DAAAAGAIAAGAAGVAADDAKSEPVAAVAADGGGRPSRRTAPSHAPAGRFPSGRRPWL
                     IAVGVVALVALAGMVFAATMNDPLPTRAAARDAAREAAENVLPPETPPTFADAWSGLI
                     AAIGSAQATEDISDHAAEELLKDADELLGAYREGDTDRVGESLQHLDEHLAKAVEDEE
                     ISPAAADAVETSISDLVVALQDEGALDSAPTGPTGDEQGSDEHGGSPPYGEAKGHED"
BASE COUNT          455 a         1132 c         1100 g          457 t
ORIGIN      
        1 cgctcgcggt cggcgacgcc cgcggcgatg cagtggtcga cgccggccat cacgtcctgg
       61 aaatccatcc cgcccgagtc gccgatcacc gcctgcgcga acgcgtgccc gcggccgatg
      121 ctccctcgcg ggttcggcag caggcaggcg tacccggcgc tcgcgagcag gaccgcgttc
      181 ggctccgagt ccgagaagaa tgccccccag ttccaggtgg ggccaccgtg cacgagcgtt
      241 agcagcggca gcggagcctt tgcaccgcgc ggcgtcatca acaggccctc gatctcgatc
      301 ccgtcgggcg tggtccaccg gatcgtgcgc acctcgggga acacggtgtc ggcgatcacg
      361 tcgtcgttga acgaggtgaa ccgtgtccac tcccctgtgt cgtgatcgaa ccgcgcgagc
      421 tcgggcggac gcccgtgcgc ctgatgcgtg gtccacacga cggagcctcc gccggcgatc
      481 gtgcatctcg gcttggtcac ctcgtcgccg atgaacgcgt ccccgcgcca ccgctcctcg
      541 acggcgccat cgagcgagat ccgcccgcac gccgtcccgg tcccgtcgca ccgcgcgtac
      601 cagagcgact cgtcgtcgat ccattcagcg aggccgacgg tctgcagacc cggccaggga
      661 tcggtcgtcg tcccgtcggc gaggtcgacg atcatcacgc tgccgctcag cagcccgggg
      721 tcgctcgagt agccctctac cacggccgcg cggcgcccgt ccggcgagag cgcgaggccc
      781 tcgagcgacc aggtcggccg gtagagggtt tgcgcggtgc gttcggcgag gtcgagccgg
      841 gcgaccaccg agcggtacca ccccgccgtg ctcggatcct ccgagacgag ggcgaccagg
      901 atgtcgtcgg gggccccgtc gaactcccac acgctcatgc ccggcggccc gacctccgtg
      961 acgtcgccgg aggcgaggtg gatctcgaag agccgccgac gggcgtcacc cggcttgatg
     1021 atcgtggggt cgggggcggg ttcggcgccg cgcacggctc gagcgctcca gtccaggccg
     1081 tagcagccgg ggtcggccgc gagcaccagc aggagatcgc gcgagcgtga ccacgagacc
     1141 gcctcggcag aaccgtgcag gttcgcggcg agcaccggct ccgcgccgac ggccatcgtg
     1201 tacgggagct ggtggccggg ggtgatccga tcggacagga acgccagccg cgagccgtcc
     1261 ggggaccacg ccgggctccg gtcgctccac acacccgtcg tgacctggac cggttcgtcc
     1321 gagtcccgct cgacgaggta gaggtcactc ggcgggtgcg acccgacctc tgcgaccgtc
     1381 gagtgcagtg cgaacgcgac cctcgacccg tcaggagaga acgcgagctc cgctggcgtc
     1441 cgaacgtcca gcaggcgctc gtcgatcggg ccgtggagac cttgggtcgc actcacggcg
     1501 cgccagcgta tctccctggc tccggccttg gagaggagtc tcgaacgtga acgagggctg
     1561 atcggccggt gcatactgac tcgatgcatg cgttgcgcgg cggacgaacg atcgcgctcg
     1621 tggtgatcgt ggcagcgctc gtggcctgcg gcggcgcgcc agaggttcag gacccgaagc
     1681 cggctcccga gccggcgagt ggggggatac cgtggccggc gccttcacac ccgctcgacc
     1741 tgaccgtcgc cgccggcctc gagccgacga cccacgagtt cctcgatttc cacgtccatg
     1801 cgcatctcga cgtgttcgtc aacgggtcac ccctcgaggt gccggcaggg atcggcatca
     1861 acatcgagga cccggccgtc cacatcgtgg aggatgacct cgggacgacg tatgggggta
     1921 tcgatcctcc ctgtgcgcag ccctgcatct cgccgttgca cacgcatggg accgacggca
     1981 tcttgcacac cgagtcggca gttgaccatc cgaacacgct cgggcaattc ttcaccgagt
     2041 ggggtgtgga actcaccgac acgtgcgtgg gcgggtattg ctcgcccggc gcatcgatcc
     2101 aggtgttcgt gaacggggaa ccgtacgagg gagaccccgc ccagatccag ctcatagatc
     2161 ggttggagat cgccatcgtg atcgggtcgc cgcccgagga ggtcccctcc acgttcccag
     2221 aggcgtaagc accgctccat aagcccacaa gtgttcatgg agcccgccag acagagcacc
     2281 gatatgcgct cgaaccccta gatgagagac gagccctagt cttcgtggcc cttggcttcg
     2341 ccgtacggcg gcgagccgcc gtgctcgtcg ctcccctgct cgtcgccggt cgggccggtc
     2401 ggcgccgagt cgagggcgcc ctcgtcctgc agcgcgacca ccaggtccga gatcgacgtt
     2461 tccaccgcgt cggcggccgc gggtgagatc tcctcgtcct cgacggcttt ggccaggtgc
     2521 tcatcgaggt gctggagcga ctccccgacc ctgtcggtgt cgccctcgcg gtaggcgccg
     2581 agcagctcgt cggcgtcctt gaggagctcc tcggcggcgt gatcggagat gtcctcggtc
     2641 gcctgcgccg acccgatcgc ggcgatgagc ccggaccacg cgtccgcgaa cgtgggcggc
     2701 gtctccggcg ggagcacgtt ctcggctgcc tcccgtgcgg cgtcgcgtgc cgccgccctc
     2761 gtcggcaacg ggtcgttcat ggtcgcagcg aacaccatcc cggcgagcgc gacgagcgcg
     2821 acgactccca ccgcgatcag ccaggggcgg cgcccactgg ggaaccggcc agcgggagcg
     2881 tgcgagggcg cggtccgccg cgaaggccgt ccaccgccat cggcggcgac cgcggcaacc
     2941 ggctcgctct tcgcatcgtc tgcggcaacg ccggccgcac cggccgcgat cgcaccggcg
     3001 gccgccgcgt cgacgggggc gaccggctgg gtctcggccg cgacgacggt ctcgagccgc
     3061 atcgtgtcgc ctccccgggc gagggggacg gcgagcaggg cggcctcgag ctcggcggcg
     3121 gacgcgaacc ggcgcttcgg gtcc
//
LOCUS       DATBRK010000117         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2616339, whole genome shotgun sequence.
ACCESSION   DATBRK010000117 DATBRK010000000
VERSION     DATBRK010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2616339"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VIH70_03495"
     CDS             <1..966
                     /locus_tag="VIH70_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8115458.1"
                     /translation="RRLVTKLFTRGAVARYEGFVREQARLVLDRGLPKGEFDFVEEIS
                     RELPIRVLARIMGVPDEDLPMCVELGDAMIAQADPEYSRAVIDKEDTSEYRLLPFRSP
                     AAVELMAYGHRLAEERRAEPRDDLVTKLTQAEVEGERLSTREFDNFFCLLIVAGNETT
                     RHAITHGVRALVDHPDQWRRLREDPGIMPLAAEEILRYGSPTMHFRRTATRDIELRNT
                     TIREGDKVVVWFVSANYDEEVFRDAERFDVGRDPNSHMAFGSGGPHVCLGGHLARLEI
                     RVMFEELVPRLSSLEITGPIERLRSNFINGIKHMPVRVELAGRAG"
     gene            971..1402
                     /locus_tag="VIH70_03500"
     CDS             971..1402
                     /locus_tag="VIH70_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8115459.1"
                     /translation="MANGLTSESSITIDAPSGRVWEALTTPELIKEWFFGVDTETDWK
                     VGGPIVHRGEYQGSPYQDKGTILAFDPPRSFVHSHWSPVSGLPDSPENYQEVSWNLAE
                     HDGRTELTVREVNLPSEEARAVSDKAWSGALQALKDLVENA"
     gene            complement(1465..2958)
                     /locus_tag="VIH70_03505"
     CDS             complement(1465..2958)
                     /locus_tag="VIH70_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEY8115460.1"
                     /translation="MTTIVPRWEWRAFGRSFGAAEDALVPISPERTEEGDEIYLVSGV
                     SANVKVRDGLMDVKVLREVDGDGLERWEPVMKQGFPLAAKDVSEVFGALDVPVPDLTP
                     ATYTLDAFLNELIEPNDLASVVNVHKRRNRFTVGGCMAELADVVADGRPTRTIAIESE
                     DAAAVIAAVRAIGLGGYTNTSYPRGLAALIDDEPARSAVIDVGTNSVKLHIGERDGSG
                     GWHTILDRAEITRLGEGLHERGEIGREALDRTLTAIQAMLDEAEREGARAVAAVGTAG
                     LRMARNGDDVLASIRERTGVGVEVISGEVEARLAYLAARAGLDLGEGSLVVFDTGGGS
                     SQFTFGQGSRVDERFSVEVGAVRYTERFGLADSVTPEALSEAMAAIADDLSRLDGRPP
                     PDALVGMGGAVTNIAAVHHGLTTYDPEVIQGTVMDRVEIARQIELYRSRDADERRAIA
                     GLQPKRSDVILAGACIVATVMDKLGAPTVTVSDRGLRHGLLVDRFGA"
BASE COUNT          487 a         1134 c         1058 g          491 t
ORIGIN      
        1 cgccggctgg tcaccaagct cttcacgcgg ggcgcggtcg cccggtacga ggggttcgtt
       61 cgggagcagg cccggctcgt cctggatcga ggtctgccca agggcgagtt cgacttcgtc
      121 gaggagatct cgcgggagct cccgatccgc gtcctcgccc ggatcatggg cgtccccgac
      181 gaggatctcc cgatgtgcgt cgagctcggc gacgccatga tcgcccaggc cgatcccgag
      241 tactcgcgcg ccgtgatcga caaggaggac accagcgagt accgcctcct ccccttcagg
      301 agcccggcgg cggtggagct catggcctac ggccaccgcc tcgccgagga gcgacgggcc
      361 gagcccaggg acgacctggt caccaagctc acccaggccg aggtcgaggg cgagcgactg
      421 tccacgcggg agttcgacaa cttcttctgc ctgctgatcg tcgccgggaa cgagacgacg
      481 cgccatgcca tcacccacgg cgtgcgtgcc ttggtcgatc acccggacca gtggcgacgc
      541 ctgcgcgagg atccgggcat catgccgctc gccgccgagg agatcctccg gtacggctcg
      601 ccgacgatgc actttcgtcg gaccgcgacg cgcgacatcg agctccggaa cacgacgatc
      661 cgcgagggcg acaaggtcgt cgtctggttc gtgtcagcca actacgacga ggaggtcttc
      721 cgggacgccg aacgtttcga cgtcggccgc gacccgaact cgcacatggc gttcggcagc
      781 ggcgggccgc acgtgtgcct gggcgggcac cttgcccggc tggagatccg ggtgatgttc
      841 gaggaactcg tcccacgcct gtcgagcctg gagatcaccg gcccgatcga acggctccgc
      901 tcgaacttca tcaacggcat caagcacatg ccggtgcgtg tcgagctcgc aggtcgggcg
      961 ggataaacgg gtggcgaacg gtctgacatc ggagagctcg atcacgatcg acgcaccttc
     1021 ggggcgcgtg tgggaggcgt tgacgacgcc cgaactcatc aaggagtggt tcttcggggt
     1081 cgacaccgag acggactgga aggtcggcgg cccgatcgtt catcgaggtg agtaccaggg
     1141 cagtccgtac caggacaagg gcacgatcct ggcgttcgac cctccgcgat cgttcgtgca
     1201 cagccattgg agcccggtct cgggtcttcc cgacagtccc gagaactacc aggaggtgtc
     1261 ctggaacctt gccgagcacg acggccgaac cgagctgacg gtccgggagg tcaaccttcc
     1321 gtccgaggaa gcgagggccg tctcggacaa ggcctggagc ggcgcgctgc aagccctcaa
     1381 ggacctcgtc gagaacgcat agggccccga ccggtcatcg gaccggcgct ctgtcgcgtc
     1441 ggtgttcgcc acggcaactc caccttatgc gccgaaccga tcgaccagca ggccgtgccg
     1501 gagccctcga tcgctgaccg tcaccgttgg cgcgcccagc ttgtccatga cggtcgccac
     1561 gatgcacgcg ccggcgagga tcacgtccga gcgcttgggc tggagccccg cgatcgctcg
     1621 gcgctcgtcg gcgtcccgcg agcggtagag ctcgatctgg cgcgcgatct cgacccggtc
     1681 catgaccgtc ccctggatga cctcgggatc gtaggtggtc aggccgtgat gcacggctgc
     1741 gatgttggtg acggcgccgc ccatccccac gagcgcgtcc gggggcgggc gcccgtcgag
     1801 gcgcgagagg tcgtcggcga tcgccgccat tgcctcgctc agcgcctcgg gcgtcaccga
     1861 atccgcgaga ccgaaccgct ccgtgtagcg cacggcgccg acctcgacgc tgaagcgctc
     1921 gtcgacccgc gagccctgcc cgaaggtgaa ctggctgctc ccgccgcccg tgtcgaagac
     1981 gaccagcgac ccctcgccca ggtcgaggcc ggcccgcgcc gcgaggtagg cgagccgcgc
     2041 ctccacctcg cccgagatca cctcgacgcc gacgccggtg cgctcgcgga tcgaagcaag
     2101 cacgtcgtcc ccgttgcgag ccatgcgcag acccgccgtg cccaccgcgg cgaccgctcg
     2161 agcgccctcg cgttcggcct cgtcgagcat cgcttggatc gccgtcaacg ttcggtcaag
     2221 cgcctcgcgc ccgatctccc cccgctcgtg gagcccctcg cccaggcgtg tgatctcggc
     2281 tcggtcgagg atggtgtgcc accccccgct gccgtcgcgt tcgccgatgt ggagcttcac
     2341 cgagttcgtc ccgacgtcga tgacggcgct gcgcgccggc tcgtcgtcga tcagcgcggc
     2401 gagcccccgc ggatagctcg tgttcgtgta gccgccgagg ccgatcgcgc gaacggcggc
     2461 gatgaccgcg gcggcatcct ccgactcgat cgcgatcgtc cgcgtgggcc gtccgtccgc
     2521 gaccacgtcg gcgagctcgg ccatgcagcc gccgaccgtg aagcggttcc gtcgcttgtg
     2581 gacgttcacc acgctagcaa ggtcgttggg ctcgatgagc tcgttcagga acgcgtcgag
     2641 cgtgtaggtg gccggcgtga gatcgggcac ggggacgtcg agcgccccga agacctcgga
     2701 cacatccttc gccgcgagcg ggaacccctg cttcatgacc ggctcccacc gctccaggcc
     2761 gtcgccgtcg acctcccgaa ggaccttgac atccatgagg ccgtcgcgaa ccttcacgtt
     2821 cgcgctcact cccgagacga ggtagatctc atcgccttcc tcggttcgct cgggactgat
     2881 cgggaccagg gcatcctcgg cggctccgaa gcttctgccg aaagcccgcc actcccagcg
     2941 cgggacgatc gtcgtcatcg aacgcccctc gaggtctccg cgcgcatcgc tcgcaaggct
     3001 agcgcacctc cctgcgccgg ccctcactca gatgagcggc tcccgcgatc aaccggtagc
     3061 atcggcgacc cagtgagcga acgagcggtc gcggcggggc ggagtgccgc cggtgtggtg
     3121 ctcctcacgc tgtgcgccgg ccagttcctg atggccctcg acagctcggt
//
LOCUS       DATBRK010000118         9863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1749138, whole genome shotgun sequence.
ACCESSION   DATBRK010000118 DATBRK010000000
VERSION     DATBRK010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749138"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VIH70_03510"
     CDS             <1..213
                     /locus_tag="VIH70_03510"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEY8115461.1"
                     /translation="VPYLNFYLANNGGLVMPIAERPEDEQALEQIAKMLPDREVVPVP
                     GNCINEGGGGPHCITQQRPAGVAVPA"
     gene            215..517
                     /locus_tag="VIH70_03515"
     CDS             215..517
                     /locus_tag="VIH70_03515"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEY8115462.1"
                     /translation="MYLSPTEEDRLRIFAAAELARRTLELGLALNAPEATALICDEMH
                     MAARSGASFEEVVEAGRRAVAVDQLIPGVAELIEEVRLEVLLDEGSRLVVLRRPWG"
     gene            514..768
                     /locus_tag="VIH70_03520"
     CDS             514..768
                     /locus_tag="VIH70_03520"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEY8115463.1"
                     /translation="MSDPIELAPGRDRIRLEVTNTSARVVRVSSHYPFWRTNRRLQFD
                     REAARGFRLDVPAGSSVRFAPGETREVTLVRYGGHGGSDG"
     gene            761..2422
                     /locus_tag="VIH70_03525"
     CDS             761..2422
                     /locus_tag="VIH70_03525"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016824090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEY8115464.1"
                     /translation="MAELSRAEYARRYGPTTGDRVRLGDTDLWIRIEDDHVGYGDEPI
                     WGYAKNFRSRMTQFDRATPESELDMLIAGVIVVDPFLGIVKTNIGIKDGRIDGIGRAG
                     SPDISDGVELQIGPSTWPVNGYGLIATAGAIDSHVHLLTPRLVPVALSAGVTTLITAG
                     FEEPPYVMEVTYRAFEGLPVNLGLQASARTDVPGQVDRVIDAGACGLKIHEDWGAYPE
                     IIDATLERAEAHDVAVCLHTDGLNESCELEDTVEAIAGRVVHAYHVEGVGGGHVPDVI
                     GLVREPNVVCSSTTPTLPYGRATAAEHLEMILAVHGGSAELPEDVEAARERIHPATMA
                     AEGPLHELGAISIVNSDSQGMGRIGETIRRTWQLAHVMKAWSGDEGDDNARVLRYLAK
                     VTAEPARVHGIEADVGSLRPGRLADIVLWRPTAFGVKPELTLKAGAFAWGALGQGNAT
                     VEGVEPVRYGGHWGALGDAAAALSTTFVSRAALEGGIRERLGSRRRFTAVTGTRSATR
                     GSLVHNTAVLPVEVDPGDGTVTLDGRVLAIEPVGEVPLSRRYLLA"
     gene            complement(2535..2990)
                     /locus_tag="VIH70_03530"
     CDS             complement(2535..2990)
                     /locus_tag="VIH70_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8115465.1"
                     /translation="MGARARRDALDAAARGTRLDRLILDTTALVAAERGRRELDEVIA
                     DDDDVAIAAISAAELLVGVELAGARQRDRREGFVQQALSTLTIEDYTLATARAHSRLL
                     AEVRRSGRPRGAHDLIIAATSVSAERILVTADARGFEDLPGVEVRLLER"
     gene            complement(2924..3175)
                     /locus_tag="VIH70_03535"
     CDS             complement(2924..3175)
                     /locus_tag="VIH70_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY8115466.1"
                     /translation="MKDISATEAARRFSELLDAVEHRHETFVVTRQGRSVARITPAVA
                     ATGRAVKDLLLAHRPDATWGRELGVMRSMLLPEERDWTA"
     gene            complement(3223..3582)
                     /locus_tag="VIH70_03540"
     CDS             complement(3223..3582)
                     /locus_tag="VIH70_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115467.1"
                     /translation="MDLLVVSINAVVVAAVGGIVAWLGKGRFDATDRRFEALDHRIDR
                     LETRLDGRIDGLAGRFDGLEERVDGWFERLETRFDGFEARTDRRFDTLQSSVDAMRSD
                     ITQVALAVGIRPRAQNG"
     gene            3749..4381
                     /locus_tag="VIH70_03545"
     CDS             3749..4381
                     /locus_tag="VIH70_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8115468.1"
                     /translation="MKLTTVTIVSVDGVMQGLGAPDEDRSGGFERGGWVTPLFDNEAI
                     TFLNQVYERADAFLFGRRTYEIFADSWGAVEDPGINPIASALNAQPKYLVSTTLTEPR
                     WANTTVLTGDIAAAIGELKAKPGGELQVHGSGSLVRWLFDNQLVDEINLLTAPVVVGQ
                     GTRLFPDTGPDIALDLVDSRSTPKGVTIQVYRPAGRPQYGTATVDLKHVT"
     gene            4464..5201
                     /locus_tag="VIH70_03550"
     CDS             4464..5201
                     /locus_tag="VIH70_03550"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEY8115469.1"
                     /translation="MDASRSEAPHRVRSTSRIADAIVALLVTQILLLGVEALALFNRI
                     RLLERAREGHFVTLAEAEQADNRVIASVVIWLVVFAVTAIVWCVWQHRAQRNAIELAY
                     GKLEFTPGWAVGWWFVPFANLVKPFQTVRELWKASHGGHNGQVVATWSVIGWWWALWL
                     IASVLERLTVRVGSRNDLSDLIRSDTTEVVANGVMVVAAILAIMIVRSVVALQGRAVA
                     APVLGPLPPMPIAGVPMPAPPPPLPAG"
     gene            5327..5734
                     /locus_tag="VIH70_03555"
     CDS             5327..5734
                     /locus_tag="VIH70_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HEY8115470.1"
                     /translation="MTAWTDEELGRIGEAQELQLASERPDGTLRKYVTMWVVRLEDQL
                     YVRSAHGADNPWFRRAKASGVGRTRTGGLERDVTFADPAPDVHPDIDAAYRGKYDRYG
                     PEIVGTVVGSLAHAVTIRLVPRSPGLFRPSGVR"
     gene            complement(5727..6737)
                     /locus_tag="VIH70_03560"
     CDS             complement(5727..6737)
                     /locus_tag="VIH70_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8115471.1"
                     /translation="MGFRLRAGRGTNLALLVLLIGALVTGALAFAVGGGWAFWLAAHG
                     AVGLALVLLAPWKSVISARGLRRARPGTAASVLLAVLVVLTVASGIAHAVGVWTVGLA
                     MQVHVGAALVSIPLAIWHVVARRVRPRPSDLSRRSLLRAGAVAAGGVVAFGAIEGLVR
                     ATGLPGSDRRATGSYERGSFDPAEMPVTQWLDDEPPEIDPADWRLIVRTSEGESTLSF
                     EELTGFSDRVRATVDCTGGWFATQDWTGVRLDRLLEADGDVRSFVVVSATGYGRRFPI
                     ADAPSMLLATRAGERPLSVGHGFPARLVAPGRRGFWWVKWIERIETSGTPWWWQSPFP
                     LT"
     gene            complement(6827..7822)
                     /locus_tag="VIH70_03565"
     CDS             complement(6827..7822)
                     /locus_tag="VIH70_03565"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY8115472.1"
                     /translation="MAIKLALAGDTMLGRGVADRLAMSSPESLFAPEIVEIANEADLF
                     VVNLECCISQRGTRWAAPGKAFFFRAPPSAAEALAVLGVDCVSLANNHALDFGEHALQ
                     DTFDHLARVGIAWAGAGPDVDRAREPAVLSAGAFRLGVLALCDHPEDFAAGPGRPGVA
                     FADLKRGVPDWVSAAVRSVEGDAVLVTPHWGPNMVRSPVPHVRRAARALVGAGAALVA
                     GHSAHVFHGVQGRVLFDLGGFIDDYAVDPVLRNDLGLLWLVTLDARGPLSLEAVPLTL
                     GFCRTGLAHGADAAWVEQRFVDACREMGTEVIAEDRRLKMTFDATVRRTGRSGFG"
     gene            complement(7827..9224)
                     /locus_tag="VIH70_03570"
     CDS             complement(7827..9224)
                     /locus_tag="VIH70_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY8115473.1"
                     /translation="MSDTLSKHAQTGPMVAAQAGEGRLARPPVAVVLAAGRSERLADV
                     TGGGSKALVRVGGLSLAGRAVRSLLAAGIERVVVVVGYQAGPVATVVNRIAPGRARAV
                     FAENWELGNGASLAAAEPHLAEEGSFLLVTTDHVFGEGALEPLLSASGYGVLVDHAPD
                     PKAWAEGTRVRLHEEHAIAFSKEFEDPSIDCGAFLLPVAIFEAQRRAAARGDDSLAGA
                     VTELARTDPLEALPLPQGAWWQDVDTPADLRRAAERLRRSLTKVADGPVAHYLNRPIS
                     TRLSMALAHLPLHPDVVSLVAFLVTVAGAWSLAMGAGVAGGLLVHAGSLLDGVDGEIA
                     RLQVRAGPAGALLDGVLDRLADACVIGGLALWAIEAGADPSVAVTLAVAATTGAMLSM
                     ATKDRITALGLPPAPERSIGFVFGGRDARLLIVTVFAILGLPSVALAAVAITSGASLA
                     IRLYLTRRVVRRQIR"
     gene            complement(9302..9625)
                     /locus_tag="VIH70_03575"
     CDS             complement(9302..9625)
                     /locus_tag="VIH70_03575"
                     /inference="COORDINATES: protein motif:HMM:NF040039.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6256 family protein"
                     /protein_id="HEY8115474.1"
                     /translation="MLRAVLPPLITAYALFAAMVALAWRRPVARPIARGEGSTRVRLG
                     RVVRTTVGGYVAFLAVVLIFHVWLAKEPDAFVNALWGGAFLCGILLSVAWGWSLLGRG
                     SRRHS"
     gene            complement(9632..>9863)
                     /locus_tag="VIH70_03580"
     CDS             complement(9632..>9863)
                     /locus_tag="VIH70_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6186 family protein"
                     /protein_id="HEY8115475.1"
                     /translation="EVTGMRAIAVVGWLVIVAALLVWQGIGLVHGPEWPTLSDFFRSF
                     MTVPLGRFILFGLWLWLGWHLFIRGWSFFLRS"
BASE COUNT         1620 a         3460 c         3434 g         1349 t
ORIGIN      
        1 gtcccgtacc tcaacttcta cctcgcgaac aacggcggcc tcgtgatgcc gatcgccgaa
       61 cgaccggagg acgagcaggc gctcgagcag atcgcgaaga tgctcccgga ccgcgaggtc
      121 gtgcccgtcc cgggcaactg catcaacgag ggcggcgggg gaccgcactg catcacccag
      181 cagcgtcccg cgggcgtcgc cgtcccggcc tgagatgtat ctgagcccga ccgaggagga
      241 tcggctgcgg atcttcgccg cggcggagct cgcgcggcgc acgctcgaac tcgggctcgc
      301 gctgaacgcc ccggaggcga ccgctttgat ctgcgacgag atgcacatgg cggcgcggtc
      361 cggcgcctcg ttcgaggagg tggtcgaggc cggccggcgg gccgtcgcgg tcgaccagct
      421 catccccggc gtggccgagc tgatcgagga ggtccgactc gaggtgctcc tcgacgaggg
      481 gagcaggctc gtcgtgctgc ggaggccgtg ggggtgagcg acccgatcga gctcgcgccc
      541 ggccgggacc ggatccgcct cgaggtgacg aacacgtccg cccgcgtcgt ccgcgtctcc
      601 tcccactacc ccttctggcg gacgaaccgc cgtctgcagt tcgaccgcga ggccgcccga
      661 gggttccggc tcgacgtccc cgcgggctcg tcggtcaggt tcgcgccggg cgagacccgc
      721 gaggtcacgc tcgttcgcta cggcggccac ggaggctccg atggctgagc tgtcgcgcgc
      781 ggagtacgcc cggcgctacg gaccgaccac gggcgaccgc gtgcgcctgg gcgacaccga
      841 cctgtggatc cggatcgagg acgaccacgt cggctacggg gacgagccga tctgggggta
      901 cgcgaagaac ttccgctcgc ggatgacgca attcgaccgc gccactcccg agagcgagct
      961 cgacatgctg atcgccggcg tgatcgtcgt cgatccgttc ctcggcatcg tgaagacgaa
     1021 catagggatc aaggacggcc ggatcgacgg gatcggccgc gcgggcagcc ccgacatcag
     1081 cgacggcgtc gagctgcaga tcggcccgag cacgtggccg gtgaacgggt acgggctcat
     1141 cgcgacggcc ggcgcgatcg acagccacgt gcacctcctc accccgcggc tcgtcccggt
     1201 ggcgctctcg gcgggcgtca cgacgctgat cacggcaggc ttcgaggagc cgccgtacgt
     1261 gatggaggtg acgtaccgag cgttcgaggg gctgccggtg aacctgggcc tgcaggcgag
     1321 cgcccgcacc gacgtccccg gccaggtcga ccgcgtgatc gatgcgggcg cctgcggcct
     1381 caagatccac gaggactggg gcgcgtaccc cgagatcatc gatgccacgc tggagcgcgc
     1441 cgaggcgcac gacgtcgcgg tgtgcctgca cacggacggg ctcaacgagt cctgcgagct
     1501 cgaggacacg gtcgaggcga tcgcgggccg cgtcgtccac gcctaccacg tggagggcgt
     1561 gggcgggggt cacgtgcccg acgtgatcgg cctggttcgc gagccgaacg tcgtctgctc
     1621 ctcgacgacc ccgacgctcc cctacggccg cgcgaccgcc gccgagcacc tggagatgat
     1681 cctcgccgtc cacggcggct cggccgagct gcccgaggac gtcgaggccg cgcgcgaacg
     1741 gatccatccg gcgacgatgg cggccgaggg cccgctgcac gagctgggcg cgatctcgat
     1801 cgtgaacagc gactcgcagg gcatgggccg gatcggcgag acgatccggc ggacctggca
     1861 gctcgcccac gtcatgaagg cgtggtcggg ggacgagggc gacgacaacg cccgagttct
     1921 gcgctacctg gccaaggtca cggccgagcc ggcgcgggtc cacgggatcg aggccgacgt
     1981 gggctctctt cgtcccggcc gcctggccga catcgtcctg tggaggccga cagcgttcgg
     2041 cgtcaagccg gagctgaccc tgaaggccgg cgccttcgcc tggggcgccc tcggccaggg
     2101 gaacgcgacg gtggaaggtg tggagccggt caggtacggc gggcactggg gagcgctcgg
     2161 cgacgcggcc gccgcgctct cgacgacgtt cgtgtcgagg gcggctctgg agggagggat
     2221 ccgtgagcgg ctggggagcc ggaggcggtt caccgcggtg accgggacgc gttccgccac
     2281 gaggggttcg ctggtccaca acacggccgt gcttcccgtg gaggtcgatc ccggtgacgg
     2341 gaccgtgacg ctcgatgggc gcgtcctggc catcgagccg gtcggggagg tgccgctcag
     2401 tcgcagatac ctgctggcct gatggggccg ggagtcaggg tcaggggaaa tggggactgc
     2461 caccaccaag gcgttccgct cgtctcgatg cgttcgatcc atctccccac cagccactcg
     2521 gagcgacggc cgattcatcg ctcgaggagg cgaacctcga ccccgggcag gtcctcgaac
     2581 ccacgagcgt cggcggtcac caggatccgt tcggcgctca ccgacgtcgc ggcgatgatc
     2641 aggtcgtggg cgccacgcgg gcgaccggac cgacggacct ccgccaagag tcgactgtgg
     2701 gctcgggccg tcgccagggt gtagtcctcg atcgtgagtg tcgacagcgc ctgttggacg
     2761 aacccctctc gtcggtcgcg ctgccgtgcc ccggccagct cgaccccgac gagcaactcc
     2821 gccgccgata tggccgcgat cgcgacgtcg tcgtcgtcgg cgatcacctc gtccagctcg
     2881 cggcgcccgc gttcggcggc cacgagggcg gtcgtgtcga ggatcaggcg gtccagtcgc
     2941 gttcctcggg cagcagcatc gagcgcatca cgccgagctc gcgcccccac gttgcatccg
     3001 ggcggtgtgc cagcaggaga tccttcaccg cacgtccggt ggcggcgacg gccggggtga
     3061 tccgggccac cgaccggccc tggcgggtca cgacgaaggt ctcgtgtcgg tgctcgacgg
     3121 catcgagcaa ctccgagaat cggcgagccg cctcggtggc ggagatgtcc ttcacatgaa
     3181 tcagattatc agacaaggat atggtgacgc cctacaagct cgtcagccgt tctgggctcg
     3241 aggacggatc ccgacggcga gcgccacctg cgtgatgtcg gaacgcatcg catccacgga
     3301 cgactggagg gtgtcgaacc gacggtccgt gcgtgcctcg aacccatcga accgcgtctc
     3361 cagccgttcg aaccaaccgt ccacgcgttc ctcgagcccg tcgaaccgtc ccgccagccc
     3421 atcgatccga ccgtccaggc gcgtttcgag tcgatcgatc cggtgatcga gggcctcgaa
     3481 cctgcggtcc gtcgcgtcga aacggccctt gccgagccag gccacgatgc cgccgacggc
     3541 ggcgaccacg accgcgttga tcgaaacgac gagcaggtcc atcgctggtg cgtcctccgg
     3601 gggagagcgc ctcgatggac ggaggctaac accgagcacc gacgcttgcg caaacccgat
     3661 cgacgactct gctccgaccg atgagatggc gcctcggtcc ggttaaagtc gaacgacgac
     3721 aagacccagc gagaagggag atgcgacgat gaaactgacg accgtcacca tcgtctccgt
     3781 cgacggagtg atgcagggac tcggcgcgcc ggacgaggac cgcagcggcg gcttcgagcg
     3841 cggcggatgg gtcacgccgc tgttcgacaa cgaggccatc acgtttctca accaggtcta
     3901 cgagcgcgcc gacgcgttcc tgttcggccg ccggacctac gagatcttcg ccgactcctg
     3961 gggcgcggtg gaagatccgg gcatcaaccc catcgcgagt gccttgaacg cccagccgaa
     4021 gtacctcgta tcgaccacgc tcacggaacc acgctgggcg aacacgaccg tcctcactgg
     4081 tgacatcgcg gcggccatcg gcgagctgaa agccaagccg gggggtgagc tgcaggtgca
     4141 cggcagcggc agcctagtcc gctggctgtt cgacaaccag ctcgtcgacg agatcaacct
     4201 gctcacggcc ccggtggtcg tcggccaggg cacgcggctc ttccccgaca ccggcccaga
     4261 catcgcgctc gacctggtcg actcgcggtc caccccgaag ggcgtaacga tccaggtcta
     4321 ccggcccgcc gggcgtccgc agtacggaac ggccaccgtc gacctgaagc acgtgacata
     4381 gatccgccgt cctaaggctc gttcggctcc ggcccgatac atctgtgcag acctgatcgc
     4441 acctctggag ttcgggcatg aacgtggatg catcccgatc ggaagctccc caccgggtcc
     4501 gctcgacgag ccggatcgcc gacgcgatcg tcgcattgct ggtcacgcag atccttctcc
     4561 tcggcgtcga ggcgcttgcc ctgttcaatc ggatccgcct cctcgagcgc gctcgtgagg
     4621 gccacttcgt gacgctggct gaggccgagc aagccgacaa tcgggtcatc gcgtcggtgg
     4681 ttatctggct cgtcgtgttc gccgtgaccg cgatcgtgtg gtgtgtgtgg caacaccgcg
     4741 cgcagcgaaa cgcgatcgag ctcgcgtacg ggaagctcga gttcaccccg ggatgggcag
     4801 tggggtggtg gttcgtcccg ttcgcgaacc tcgtcaagcc gttccagacg gtgcgagagc
     4861 tctggaaggc gagccatggt gggcacaacg gacaggtcgt tgccacctgg agtgtgatcg
     4921 gctggtggtg ggccctttgg ctcatcgcga gtgtgctcga gcggctcacg gtccgcgttg
     4981 gttcgaggaa cgacctttcg gacctcatcc gtagcgacac gacggaggtc gtcgcgaacg
     5041 gcgtcatggt cgtcgcggcg atcttggcga tcatgatcgt gcgctcagtc gttgctcttc
     5101 agggacgcgc tgttgccgca ccggtcctcg gccccttgcc gccgatgccg atcgccggcg
     5161 tccccatgcc agcgccgccg ccgcctctac cagccgggta gcgttggggt aaccggtctc
     5221 gccggcgacc agccgcccgg gcggcaccgt ggggttcggc ggtgagctgc tcgggttact
     5281 gtgatgaatg agtcaccgat gccgaatcgt gacgcgaggt acgccgatga ccgcatggac
     5341 cgacgaggag ctcggacgca tcggcgaggc gcaggagctg cagctcgcct cggaacgccc
     5401 ggacggcacg ctccgcaagt acgtgacgat gtgggtcgtc cgcctcgagg accagctgta
     5461 cgtacgctca gcccacggcg cggacaaccc gtggttccgg cgcgcgaagg cgagcggcgt
     5521 gggtcgcacc cggaccggag gcctggagcg ggacgtcacg ttcgccgacc ctgctccgga
     5581 cgtccacccc gacatcgacg ccgcctaccg cgggaagtac gaccggtacg ggccggagat
     5641 cgtcggaacc gtcgtgggat ctctcgccca cgcggtgacg atcaggctcg tgccgcgctc
     5701 gccgggctta tttcgaccat cgggagtcag gtgaggggga acggggactg ccaccaccac
     5761 ggcgttccgc tcgtctcgat gcgttcgatc catttcaccc accagaaccc gcgccggccg
     5821 ggcgcgacga gcctggccgg gaagccgtgg ccgaccgaga gcggccgttc gcccgccctc
     5881 gtcgcgagga gcatcgaggg agcgtcggcg atcgggaacc gtcgtccgta ccccgtcgcg
     5941 gacacgacga cgaagctccg cacgtcgccg tccgcctcga ggagccggtc caggcgaact
     6001 ccggtccagt cctgggtcgc gaaccagccg ccggtgcaat cgaccgtcgc gcgcacccga
     6061 tcggagaagc ccgtgagctc ctcgaaggac agggtcgact ctccttccga ggtccgaacg
     6121 atcaggcgcc aatcagcggg gtcgatctcg ggtggctcgt cgtccagcca ctgggtgacc
     6181 ggcatctcgg caggatcgaa cgagcctcgt tcgtacgaac cggtcgccct gcggtcggaa
     6241 ccgggaaggc cggtcgcgcg cacgaggccc tcgatcgcgc cgaacgccac gacgccgccg
     6301 gcggcgaccg cgccggcgcg cagcagactt cggcgcgaca gatcgctcgg acgcggtcga
     6361 acgcgcctcg ccaccacgtg ccagatcgcg agcgggatcg acacgagtgc ggcgccgacg
     6421 tgaacctgca tcgcgagccc gaccgtccac acccccaccg cgtgagcgat gccgctcgcg
     6481 acggtgagca cgacgagcac ggcgagcagc acggaggcgg ccgtccccgg cctcgctctt
     6541 cggagcccgc gggccgagat cacggatttc cacggcgcga gcaggaccaa cgcgagtccg
     6601 accgccccgt gcgccgcgag ccagaacgcc caccccccgc cgaccgcgaa cgcgagtgcg
     6661 ccggtgacga gcgcaccgat caggagtacg agcagcgcga ggttcgtccc gcgcccggcc
     6721 cgaagccgaa accccacggt ccgaggctac gcccactgcc cgtgttcggg gggcgcttcg
     6781 cgaggctgca tcctcgtccc acgagtctcg gttcacgcga acgtggtcac ccgaaacctg
     6841 atcggccggt tcggcgaacg gtcgcgtcga aggtcatctt caaccgtcgg tcttccgcga
     6901 tgacctcggt tcccatctca cggcaagcgt ctacaaatcg ttgctccacc catgccgcgt
     6961 ccgcaccatg tgccaacccc gtacggcaga agccgagggt cagcggcacg gcctcgagcg
     7021 agaggggtcc cctcgcgtcc agcgtgacga gccacaggag ccccaggtca ttgcggagga
     7081 ccggatcgac ggcgtagtcg tcgatgaagc ctccgagatc gaagagcacc cgtccctgga
     7141 cgccgtggaa cacgtgggcg gaatgacccg ccaccaacgc ggccccggct ccaacgaggg
     7201 ctcgggcggc ccggcgcacg tgagggaccg gcgaccgcac catgttcggc ccccagtggg
     7261 gagtcacgag gacggcgtcc ccctcgaccg atcgaacggc ggccgacacc cagtccggca
     7321 ccccccgctt caggtcggcg aaggccaccc cgggccgacc ggggccggcc gcgaagtcct
     7381 cggggtgatc acagagcgcg aggaccccca gtcggaaggc gcccgcgctc agcacggccg
     7441 gctctcgggc tcggtcgacg tcgggtccgg cacccgccca cgcgatcccg acgcgggcga
     7501 gatggtcgaa cgtgtcctgc agggcatgtt cgccaaagtc gagcgcgtgg ttgttggcca
     7561 gggagacgca gtcgacccca aggacggcaa gtgcctcggc cgcggacggg ggagcccgaa
     7621 agaagaaggc cttgccgggt gcggcccacc tggttccccg ctgggagatg caacattcca
     7681 ggttcacgac gaacaggtcg gcctcgttgg cgatctccac gatctcgggt gcgaacagcg
     7741 actcgggtga ggacatggcc aatcggtccg cgacgccacg tccgagcatc gtgtctccgg
     7801 ccagcgcgag cttgatggcc acgccgctag cggatctggc ggcggaccac gcggcgcgtg
     7861 aggtagagcc ggatcgcgag gctcgcgccc gaggtgatgg ccaccgcagc gagggccacc
     7921 gaggggagcc cgaggatggc gaacaccgtc acgatgagca ggcgcgcatc tcgaccaccg
     7981 aacacgaacc cgatcgaacg ctcgggggcc ggtgggaggc cgagtgccgt gatgcgatcc
     8041 ttggtggcca tcgagagcat cgcgccggtc gtggccgcga cggccagcgt tacggcgacc
     8101 gatggatcgg ccccggcctc gatcgcccag agcgcgagcc caccgatcac gcaggcgtcg
     8161 gcgaggcggt cgaggacccc gtccaggagt gcccccgccg gacccgcccg tacctggagc
     8221 ctcgcgatct ccccgtcgac accgtcgagg agcgaacccg cgtgaacgag gagtccaccg
     8281 gcgactcccg cacccatcgc gagactccat gcgccggcca ccgtgacgag gaaggcgacg
     8341 agcgagacga catccgggtg caggggcagg tgcgcgaggg ccatcgacag acgcgtcgag
     8401 atcggtcggt tgaggtagtg cgcgaccggg ccgtcggcga ccttcgtcag cgagcggcgc
     8461 aggcgctcgg cggcgcggcg aaggtccgcc ggcgtgtcca cgtcctgcca ccacgcgcct
     8521 tggggcagcg gtagggcctc cagcggatcg gtgcgagcga gctcggtcac cgcgccggcg
     8581 agcgagtcgt cgccgcgggc ggccgcgcgc cgctgggctt cgaagatcgc caccggcagc
     8641 aggaaggcac cgcagtcgat cgaggggtcc tcgaactcct tcgaaaaggc gatcgcgtgc
     8701 tcctcgtgca gacgcacgcg ggtgccctcg gcccatgcct tggggtctgg ggcgtggtcg
     8761 accagaaccc cgtacccgct cgccgatagc agcggctcga gtgcgccttc gccgaatacg
     8821 tggtccgtgg ttacgagcag gaaggacccc tcttccgcca ggtgaggctc ggccgccgcg
     8881 agcgatgccc cgttcccgag ctcccagttc tcggcgaaca cggcgcgagc ccgacccggt
     8941 gcgatccggt tcacgacggt tgccacgggg ccggcctgat agccgacgac gacgacgacc
     9001 cgctcgatgc ccgccgcaag caggctgcgt acggcgcgac cagccaggct gaggccgccg
     9061 acgcggacga gggctttcga gcccccgccg gtcacgtcgg caaggcgttc cgaccgacca
     9121 gcggcgagga cgacggcgac gggggggcgg gcgagcctgc cttcgcccgc ctgagccgca
     9181 accatcggcc cggtctgggc gtgcttggac agagtgtcgg acatacgcgc cccccggggc
     9241 cgtcatgacg acccctccga agcatcctcg cggcggcgcg ctctggcaac tcgaccgtcc
     9301 ctcaggagtg acgccggctt ccccttccga ggagtgacca gccccacgcc acggacagca
     9361 agatcccgca aaggaacgca ccaccccaca gcgcgttcac gaatgcatcc ggttcctttg
     9421 cgagccaaac gtggaagatc aggacgaccg cgaggaacgc gacgtatccg ccgaccgttg
     9481 tccggacgac gcgaccgagg cgcacgcggg tcgacccctc acctcgcgcg atcggcctcg
     9541 ccaccggccg cctccacgcg agggccacca tcgccgcgaa gagggcatac gccgtgatca
     9601 acggaggcag gaccgccctg agcacgttcg ctcagctccg aaggaagaag gaccaccctc
     9661 ggatgaacag gtgccagccg agccacagcc agagcccgaa caggatgaag cgtccgaggg
     9721 gcaccgtcat gaaggaccgg aagaagtccg agagcgtggg ccactcgggc ccgtggacca
     9781 gcccgatccc ctgccagacg aggagcgccg ccacgatgac gagccacccg accaccgcga
     9841 tcgcccgcat acccgtcacc tcc
//
LOCUS       DATBRK010000119         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2758784, whole genome shotgun sequence.
ACCESSION   DATBRK010000119 DATBRK010000000
VERSION     DATBRK010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2758784"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VIH70_03585"
     CDS             <1..484
                     /locus_tag="VIH70_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8115476.1"
                     /translation="LLKDPEVLILDEPANGLDPAGIVEVRKLVRRLGHEGRTVFVSSH
                     ILNEVQQTADRVAILAKGRLVATGPVHEVLTAGHATGLLVRLDDVPEGLRILGEAGID
                     ARADDGLIRVALPPADAARVTRALADRGLYLTELRADEADLETVFLELTSGASTEGAR
                     "
     gene            481..1227
                     /locus_tag="VIH70_03590"
     CDS             481..1227
                     /locus_tag="VIH70_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115477.1"
                     /translation="MSGLLASEFRRFGSRRLVKVLVAVEVIAIVATGVIVFLTQEYAL
                     VGLPDVLMGTSLVLVSVGWILGASAIGADWHAGHVTTILTWEPRRGRVQFAKIAAALA
                     GVFVLSLVIQALLGIALAVAAAGAGSTTGADAAWLAESAGVALRVAALSSIFAGFGFG
                     LASAGRNTAVALGVGFGYLVVVENLVRGLRPQWTPWLLQDNAGLFVVGSPIDFPLLGR
                     STIGAGLYLTAVGVALLLAATGLFRARDVL"
     gene            1298..>1636
                     /locus_tag="VIH70_03595"
     CDS             1298..>1636
                     /locus_tag="VIH70_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY8115478.1"
                     /translation="MLADALQTTFEDAVPLSEVEFVVVDLETTGGSPTDSWITEIGAV
                     KLRGGERTGVFETLVNPGVPIPRQITHLTGIDDLVVAGAPPIEWVLPTFAEFARGCVF
                     VAHNARFDFTF"
BASE COUNT          195 a          577 c          594 g          270 t
ORIGIN      
        1 actcctcaag gatcccgagg tcttgatcct cgacgagccg gcgaacggac tcgatccggc
       61 cgggatcgtc gaggtccgca agctggtgcg gcgactcggg cacgagggaa gaaccgtctt
      121 cgtctcgagc catatcctga acgaggtcca gcagacggcc gaccgcgtcg cgatcctggc
      181 caagggtcgg ctggtcgcga ccggtccggt gcacgaggtc ctcaccgcgg ggcacgccac
      241 ggggctgctc gtgcggctgg acgacgtgcc cgagggtctc cggatcctcg gcgaggccgg
      301 gatcgacgca cgtgcggacg acggcctcat ccgggtcgca ctcccgcccg ccgacgcggc
      361 gcgcgtgacc cgagccctcg ccgaccgagg gctgtacctg accgagctcc gcgccgacga
      421 ggcggatctc gagaccgtct tcctcgagct caccagcggc gcctccacgg agggcgcgcg
      481 gtgagcgggc tgctcgcctc ggagttccgc cgcttcgggt cgcgccgact ggtcaaggtg
      541 ctcgtcgcgg tcgaggtgat cgcgatcgtc gcgaccggcg tgatcgtgtt cctcacgcag
      601 gagtacgcgc tcgtgggtct gcccgacgtg ctgatgggca cgtcgctggt cctcgtgtcc
      661 gtcgggtgga tactcggtgc ctccgcgatc ggcgccgatt ggcacgcggg ccacgtcacg
      721 acgatcctga cctgggagcc acgacgcggg cgcgtgcagt tcgcgaagat cgcggccgcg
      781 ttggcgggcg tcttcgtcct ctcgctcgtg atccaggcgc tcctgggaat cgcgctcgcg
      841 gtggccgcgg ccggcgctgg gtcgaccacc ggtgccgacg cggcatggct cgccgagtcc
      901 gccggcgtcg cgctgcgcgt cgcggcgctg tcctcgatct tcgccgggtt cgggttcggg
      961 ctcgcgtcgg ccggacgcaa cacggcggtc gcgctggggg tcggcttcgg ctacctcgtg
     1021 gtcgtcgaga acctggtgcg gggtctgcgg ccgcagtgga cgccctggct cctgcaggac
     1081 aacgccggct tgttcgtcgt gggctcgccc atcgacttcc ccctgctcgg acgctcgacg
     1141 atcggggcgg gcctgtacct gacggccgtg ggggtcgcgc tgctgctcgc ggccaccggg
     1201 ctgttccgcg cgcgcgacgt gctctgagag agaccttgac ctccgaacac ctgttcgggt
     1261 agcctgctcc tcctcgcgcg gccccgcggg agggccggtg ctcgcggacg cgctccagac
     1321 cacgttcgag gacgccgtcc cgctgtccga ggtcgagttc gtagtcgtcg acctcgagac
     1381 caccggcggt tcgcctaccg actcatggat cacggagatc ggcgcggtga agctccgcgg
     1441 cggcgagcgc accggcgtgt tcgagaccct ggtgaacccg ggcgtgccga tcccgcgcca
     1501 gatcacgcac ctgaccggga tcgacgacct ggtggtggcc ggcgcgcccc cgatcgagtg
     1561 ggtgctgccg acgttcgccg agttcgcgcg cgggtgcgtc ttcgtggccc acaacgcccg
     1621 gttcgacttc acgttc
//
LOCUS       DATBRK010000120         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2181578, whole genome shotgun sequence.
ACCESSION   DATBRK010000120 DATBRK010000000
VERSION     DATBRK010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181578"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VIH70_03600"
     CDS             complement(<1..780)
                     /locus_tag="VIH70_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEY8115479.1"
                     /translation="MAGRIRDDDVAAVRERTDIVKLVSGYLTLKKAGHDRLVGLCPFH
                     TEKTPSFGVSPSKGLYHCFGCGKGGDAIGFLRDVEHLEFSEAVERLAKEAGIALRSEG
                     DTPAERRVASKRQALVRANEAAFELYHRTLLEAKEGEEARAYLESRGIDRDVAAEFEI
                     GYAPAYPDFLLRRLVRSLSAELLVEAGLAIKDQEGNVRDRFRRRITFPVHDLSGKAVG
                     IGARILPGGSGRDEGPKYLNSPETPVYRKAEVLYNLQRAKAS"
     gene            complement(811..1812)
                     /locus_tag="VIH70_03605"
     CDS             complement(811..1812)
                     /locus_tag="VIH70_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY8115480.1"
                     /translation="MAVPRERWEEIERDTLSPTATLASASKGREQDEPQDDLRTCFQR
                     DRDRIVHSKAFRRLKHKTQVFLAPEGDHYRVRLTHTLEVSQIARTAARALRLNEDLTE
                     AIALGHDLGHTPFGHLGEQALTPFLGRPFRHNEQSLRIVDHLENDGQGLNLSWEVRDG
                     IVHHTWSMPQPSTPEAQLVRFADRIAYVNHDVDDAVRAGVIEQIELPVAPLEVLGATH
                     SSRINTLVTDLVVRSADGPEIGLSDHVFQALDALREFLFDHVYLRDEARSEQDKAISM
                     VRSLFAHYLDHPEEVPQEYHRAPGDLPTRVADYIAGMTDRYALRIYEQLFLPQRWLL"
     gene            1918..>2370
                     /locus_tag="VIH70_03610"
     CDS             1918..>2370
                     /locus_tag="VIH70_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8115481.1"
                     /translation="MRGVRTRVLVVLAALSVLAVACGGDANDAAGGPAGTGPTAATGA
                     TDSGDDPGAGSQYGGSNGMTGAAGATGAKDADVSVSLNNYLFDPGTVKVGSGDIVAVR
                     NGNARTPHTFTVVGEDVDLELAPLTTETVKIDLAPGTYQLICRFHESLG"
BASE COUNT          351 a          824 c          822 g          373 t
ORIGIN      
        1 cgacgccttg gcgcgctgca ggttgtagag cacctcggcc tttcggtaca ccggcgtctc
       61 gggcgagttc aggtacttcg gcccctcgtc gcgcccgctg ccgccgggca ggatcctcgc
      121 gccgatcccg accgccttgc cggagaggtc gtgcaccggg aacgtgatcc ggcggcggaa
      181 ccggtcgcgg acgttaccct cctggtcctt gatcgcgagg ccggcctcga ccaggagctc
      241 cgccgagagc gacctcacca gccgccggag caggaagtcc ggataggcgg gcgcgtaccc
      301 gatctcgaac tcggcggcca cgtcgcggtc gatcccacgg gactcgaggt acgcccgggc
      361 ctcctcaccc tccttggctt ccaggagcgt gcggtggtac agctcgaacg cggcctcgtt
      421 cgcccggacc agggcctggc gcttggacgc cacacgccgt tcggcgggcg tgtcgccctc
      481 ggagcgcagg gcgatgcccg cctccttggc tagacgctcg accgcctcgg agaactcgag
      541 gtgctcgacg tcgcgcagga acccgatcgc gtcgccgccc ttgccgcagc cgaaacagtg
      601 gtaaaggccc ttggagggcg agacgccgaa cgacggcgtc ttctccgtgt ggaacggaca
      661 cagccccacc aagcggtcgt ggccggcctt cttcagcgtg aggtacccgg agacgagctt
      721 gacgatgtcc gtgcgttcgc ggaccgcggc gacgtcgtcg tccctgatcc tgcccgccac
      781 gttcctcccc tcgacctcgc ctcgcgccgg ctacagcaac cagcgttggg gaaggaacag
      841 ctgctcgtag atccgcagag cgtaccggtc cgtcatgccg gctatgtagt cggcaacacg
      901 cgtcggcaga tctcccggcg cccgatggta ctcctgcgga acctcctcgg ggtgatcgag
      961 atagtgcgcg aagaggctcc gcaccatcga gatcgccttg tcctgctccg agcgcgcctc
     1021 gtcccgcagg tagacgtggt cgaacaggaa ctcgcgcagc gcgtcgagcg cctggaacac
     1081 gtggtcggac agcccgatct cgggtccgtc ggcgcttcgg acgacgaggt cggtcaccaa
     1141 ggtgttgatg cgggacgagt gcgtcgcacc gaggacctcc aggggcgcga cgggcagctc
     1201 gatctgctcg atgacaccgg cccgcaccgc gtcgtcgacg tcgtggttca cgtacgcgat
     1261 acggtcggcg aacctgacga gctgcgcctc cggcgtcgag ggctggggca tcgaccaggt
     1321 gtgatgcacg atgccgtcgc gaacctccca ggacaggttc aggccctgcc cgtcgttctc
     1381 gaggtggtcg acgatccgca ggctctgctc gttatggcgg aacggtctcc ccaggaacgg
     1441 cgtgagggcc tgctccccca ggtgcccgaa cggcgtatgt cccaggtcgt ggccgagcgc
     1501 gatcgcctcg gtgaggtcct cgttcaggcg gagcgcgcgg gcggcggtcc gggcgatctg
     1561 gctcacctcg agcgtgtggg tgagccggac gcggtagtgg tcaccctcgg gggcgaggaa
     1621 cacctgcgtc ttgtgcttca agcgccgaaa cgccttcgag tgcacgatgc ggtcccggtc
     1681 ccgctggaag caggtccgga ggtcatcctg cggctcgtcc tgctcccggc ccttgctcgc
     1741 cgaggcaagc gtcgcggtcg gcgacagcgt gtcgcgctcg atctcctccc aacgttcccg
     1801 gggcacggcc acgcgccgat actaccgtcg cgatccgacg caccttatcc gcggacccgc
     1861 agtacgatcg gcgcttcgac gcggggttct cgcgaccccg tccccgagga ggctcggatg
     1921 agaggcgttc ggacgcgagt cctggtggtt ctcgcggcgc tgtcggtgct cgcggtggcg
     1981 tgcgggggcg acgcgaacga cgctgccggt ggtccggcgg ggaccggtcc tacggccgcc
     2041 accggagcca ccgactcggg tgacgacccc ggcgcgggga gccagtacgg aggctcgaac
     2101 gggatgaccg gcgccgccgg agcgaccggc gcgaaggacg ccgatgtctc ggtctccctc
     2161 aacaactacc tgttcgatcc cggcacggtg aaggtcggca gcggcgacat cgtcgccgtt
     2221 cgaaacggga acgcgaggac gccgcacacg ttcacggtcg tcggggagga cgtcgacctc
     2281 gagctcgccc cgctcacgac cgagaccgtg aagatcgatc tggcgccggg gacgtaccag
     2341 ctgatctgcc ggttccacga atccctcggc
//
LOCUS       DATBRK010000121         5725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_16633, whole genome shotgun sequence.
ACCESSION   DATBRK010000121 DATBRK010000000
VERSION     DATBRK010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16633"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..984)
                     /gene="murI"
                     /locus_tag="VIH70_03615"
     CDS             complement(145..984)
                     /gene="murI"
                     /locus_tag="VIH70_03615"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013170320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY8115482.1"
                     /translation="MSDPRPIGIFDSGVGGLTVARAVFDLLPNEPILYVGDNGRFPYG
                     PRPLEEIRTFALEIAAYMTERDVKMLVVACNSIEVAAIAEVADAHGVPVVGVIDPGVR
                     AAVHATRNDRIGLIGTEATVASGAYERAIAQTGADVRLFSAACPVFVEHVEAGDTSSD
                     ELLAAARGYLVPLDREGIDTLILGCTHYPMLSGLIRMVMGEDVLLVSSAEETAKDVYA
                     SLRASSLLRAAKDGESDEARYEFLTTGDPEQFRRVAHHFLGPVLGGVAPVDVRVVEGV
                     SWS"
     gene            complement(1040..1969)
                     /locus_tag="VIH70_03620"
     CDS             complement(1040..1969)
                     /locus_tag="VIH70_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HEY8115483.1"
                     /translation="MRFDDVLDAIGDTPLVGLPRLSPPGVRMWAKLEGQNPTGSLNDR
                     IAKAMVEAAEASGELTPGRTILEPTSGNTGIALAMVARRKGYGLTVVIPDNASEERIR
                     LLELFGAEIVLSPGDKGTNGSIEIARRLASDDRYYMPFQYGNPANPRAHEEGTGEEIV
                     RDLPEITHFVAGLGTGGTLTGVGRRLKRHDPRVKVIAAEPELGDLVYGLRSLDDGFVP
                     PIFDESVLDRKFLVNVGDSVRATRELTEREGIFAGISSGAVLHVGLRVASELERGDIV
                     CLLPDGGWKYLSTQAWAPELERAEKNVSETLWW"
     gene            complement(2031..2306)
                     /locus_tag="VIH70_03625"
     CDS             complement(2031..2306)
                     /locus_tag="VIH70_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEY8115484.1"
                     /translation="MARVKLPTILRSHAGGERAVDAEGTTLRELLEDLETRYPGITEM
                     VLTDDGALHRFVNVYVNDEDVRYLGSLETAVDPEDTVSILPAVAGGR"
     gene            complement(2377..3189)
                     /gene="moeB"
                     /locus_tag="VIH70_03630"
                     /pseudo
     CDS             complement(2377..3189)
                     /gene="moeB"
                     /locus_tag="VIH70_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
     gene            complement(3186..3620)
                     /locus_tag="VIH70_03635"
     CDS             complement(3186..3620)
                     /locus_tag="VIH70_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEY8115485.1"
                     /translation="MIELERAFFDEMLEHGLAAFPNEACGLLAGKEGRPVKFFAMTNH
                     DASPASYRLDPTEQLRAENDMEDEGWETFAVFHTHTHSEAYPSQTDQEHAFWPAVRSE
                     PRMAIFPGAYYLIMSLSDRSNPDLRAFRIQDTGVIEDEVAIG"
     gene            complement(3670..4725)
                     /gene="selD"
                     /locus_tag="VIH70_03640"
     CDS             complement(3670..4725)
                     /gene="selD"
                     /locus_tag="VIH70_03640"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015400493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY8115486.1"
                     /translation="MEGSRRLTSFSHGAGCGCKLGPADLETVLGTLSLPEVGADVLVA
                     ADTGDDAAVVRLPDGQALVATLDFFTPIVDDPYDWGRIAATNALSDVYAMGARPFLAL
                     NIAAWPVDDLPIEMLGRVLQGGLDVASKAGAAILGGHTITDPEPKYGMVALGLADPER
                     ITRNSTVPPDAHLFLTKPLGVGIATTAHKRGVAEPALLAAAVELMTTPNDAAADAMAE
                     VGAVAATDVTGFGLLGHLQRMLAASGVGARIDASSVPVLPGVLDLAMLDVVPGGTKRN
                     HAYLRPNVQWGKLSIPEQLVLADAQTSGGLLIATLDPEWMGQELTGRGIGWADVGVTV
                     QGRPGTIAVTGKLARGA"
     gene            4778..5617
                     /locus_tag="VIH70_03645"
     CDS             4778..5617
                     /locus_tag="VIH70_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015108491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY8115487.1"
                     /translation="MRTAVVGHVEWIEFAHVEDVPRAGDIVHATEPWEEPGGGGAVSA
                     VQLARLAGECTFYTALGDDERGTWSRRRLAGLGVRLEAATRDEPTRRGVVFVDANGER
                     TITTLGRRLEPTTDDDLPWDELEDVDAVYFTAGDPAALREARRAKVLVATSRVSDLLA
                     EADVRLDAVVGSGNDPSEAFDPARLTHTPNLLVRTDGVRGGRYETGNGRSGSYEPVPP
                     PGPVVDTYGAGDSFAAGLTFALGSGLGVEEELALAARCGAWCAAGRGPYGNQLSAADL
                     ESG"
BASE COUNT          832 a         2040 c         1984 g          869 t
ORIGIN      
        1 ggtcggggat gtcgatgtac tgctgcaggt tggccagcgt ccccgtgccg gcgtccatcc
       61 acatgttgaa cccgtcgtgc tgcaccaggt agccggaggt ggccgtgtcg gcgttcggcc
      121 aggtgccgct cgagccgagg atcgtcagct ccacgagacc ccctcgacca cccggacgtc
      181 gacgggggcg accccgccca ggaccgggcc gaggaagtgg tgcgcgaccc gtcggaactg
      241 ctcggggtcg ccggtcgtga ggaactcgta ccgagcctcg tcgctttccc cgtccttggc
      301 cgcccgcagc aacgaggacg cgcggagcga ggcgtacacg tccttggcgg tctcctcggc
      361 cgatgacacc aacaggacgt cctcccccat caccatccgg atcagccccg agagcatcgg
      421 gtagtgcgtg caccccagga tcagggtgtc gatcccctcc ctatccagcg gcacaagata
      481 cccccgcgcg gccgcgagca actcgtcgct cgacgtatcg ccggcctcga cgtgctcgac
      541 gaagaccggg cacgccgccg agaagagacg gacgtccgca ccggtctggg cgatcgcccg
      601 ctcgtacgcg ccgctcgcga cggtcgcctc ggtgccgatc aggccgatcc gatcgttgcg
      661 cgtcgcgtgc accgcggccc ggacccccgg gtcgatcacg ccgacgacgg gcacgccgtg
      721 agcgtccgcg acctccgcga tcgcggcgac ctcgatcgag ttgcacgcga cgaccagcat
      781 cttgacgtcg cgctcggtca tgtacgcggc gatctcgagc gcgaaggtgc ggatctcctc
      841 gagcggccgc ggaccgtacg ggaaccggcc gttgtcgccg acgtacagga tcggttcgtt
      901 cggcagcagg tcgaagacgg cgcgggccac ggtgaggccg ccgacgccgg agtcgaagat
      961 gccgatcggc cgcgggtcgc tcatccgatc gtccgaacgc gtcgtccgct cacccggtca
     1021 ccatcggcat cgctcccgtc taccaccaca gggtctcgga gacgttcttc tccgcccgct
     1081 cgagctcggg cgcccacgcc tgggtcgaca ggtacttcca cccaccatcg gggagcagac
     1141 acacgatgtc gccgcgttcg agctccgatg cgacgcgcag accgacgtgg agcaccgccc
     1201 ccgacgagat gccggcgaag atgccctcgc gttcggtcag ctcgcgcgtc gcccgaaccg
     1261 agtccccgac gttcaccagg aacttccggt ccagcaccga ctcgtcgaag atcggcggca
     1321 cgaacccgtc gtcgagcgaa cggagcccgt acaccaggtc cccgagctcc ggttcggccg
     1381 cgatcacctt caccctcggg tcgtggcgct tcagccgccg gccgacgccg gtgagggtcc
     1441 cgccggtccc gagaccggcg acgaagtgcg tgatctcggg caggtcccgg acgatctcct
     1501 cacccgtgcc ctcctcgtgc gctcgagggt tcgcggggtt cccgtactgg aacggcatgt
     1561 agtagcgatc gtcgctcgcg agccgccgcg caatctcgat cgagccgttc gttcccttgt
     1621 cgccgggcga gagcacgatc tcggcgccga acagttcgag cagccggatc cgctcctcgc
     1681 tcgcgttgtc ggggatcacg acggtcagcc cgtacccctt gcgccgggcg accatcgcga
     1741 gcgcgatgcc cgtgttgccc gacgtgggct ccaggatcgt gcgccccggc gtgagctcgc
     1801 ccgaggcctc ggccgcctcg accatcgcct tggcgatccg gtcattgagc gagccggtcg
     1861 ggttctgccc ctcgagcttg gcccacatcc gaacgcccgg ggggctcagg cgcggcaggc
     1921 cgacgagcgg cgtgtcgccg atcgcgtcga ggacgtcgtc gaacctcatc ccagcggatt
     1981 atcccagcgc acagggtcac gggtcgggtc cttctacgga cccggacccc tcaacgaccg
     2041 ccggcgacgg cgggcaggat cgaaacggtg tcctccggat cgaccgcggt ctcgagcgaa
     2101 ccgaggtaac ggacgtcctc gtcgttcacg tacacgttca cgaaccggtg gagcgcgccg
     2161 tcgtcggtca ggaccatctc cgtgatcccc gggtatctgg tctcgaggtc ctcgagcaac
     2221 tcgcgaagcg tcgttccctc cgcatcgacg gcacgttcgc caccggcgtg ggagcgaagg
     2281 atcgtcggga gcttcacgcg agccatgtcg aacgtccttt cgtctcgatc ctcagctcgt
     2341 cgcaaccccg tgctcggcct cgggcggcgc ctggccgggc gtgagcccgc agaatgcctc
     2401 gtagtcgatc agctccgtga tcgtgggctc cttgccacac acggggcagt ccgcgtccca
     2461 tcggacgcgg acggtctgga agtccatctc gagcgcgtcg aagatcagga gccggccgat
     2521 cagcgggtcg cccttgccgg tgatgagctt gactgcctcc gtcgcctgga tcgagcccac
     2581 gatccctggc agcgccccga acaccccgcc ctccgcgcag ctcgggaccg tgccgggcgg
     2641 cggcggttgc gggaacaggc acctgtagca cggggcgtcc tggccgggca tgaagaccgt
     2701 ggcctggccg tcccactggt agatcgaccc gtacacgagc ggcttgccga ggaactgcgt
     2761 cgcgtcgttg acgagatagc ggaccgggaa gttgtcggtc ccgtccacca ccacgtccca
     2821 cgggtcgagg acctcgaacg cgttctgtga cgagaggatc gtgtcgtacg gaacgatctc
     2881 gatcgtcggg ttgatcgccc gcaggcgttc ggccgcgctc tcgaccttcg gccgcccgac
     2941 gtcggcggtg gagtgcacga tctggcgctg caggttggac tcgtcgacct tgtcgaagtc
     3001 gaggatgccg agcgtcccca ccccggcggc ggccaggtac atcgccaccg gagagccgag
     3061 cccccccgcg ccgatgcaca ggaccttcgc gtccagcagc ttgcgctgtc cctcgccgcc
     3121 gatccccggc aggatgatgt gccgggcgta ccggcggacc tgctcctcgg tgaaggccgg
     3181 ggtcgtcatc cgatcgccac ctcgtcctcg atgacgcccg tgtcttggat ccggaacgcc
     3241 cgaaggtctg ggttcgatcg gtcggacagg gacatgatca ggtagtacgc cccggggaag
     3301 atggccatcc ggggctccga cctgaccgcg ggccagaagg cgtgctcctg atcggtttgc
     3361 gacggatagg cctcgctgtg cgtgtgggtg tggaagaccg cgaacgtctc ccagccttcg
     3421 tcttccatgt cgttttcggc tcgcagctgt tccgtcgggt cgagccgata gctggccggg
     3481 ctcgcgtcgt ggttggtcat cgcgaagaac ttgaccgggc ggccttcctt gccggccagc
     3541 agtccgcacg cctcgttggg aaacgcggcg aggccgtgct ccagcatctc gtcgaagaag
     3601 gcgcgttcga gttcgatcat tgcaggtgca acaaccgtac cagcaaggtg gtattccctg
     3661 aacggctgtt caggcacctc gggcgagttt gccggtgacg gcgatcgtcc cgggtcggcc
     3721 ctgcaccgtg accccgacgt cggcccagcc gatgcctcga ccggtgagct cctgacccat
     3781 ccactcggga tcgagcgtgg cgatcagaag cccgcccgag gtctgcgcgt ccgcgagcac
     3841 cagctgctcg gggatcgaga gcttgcccca ctgcacgttc gggcgcaggt acgcgtggtt
     3901 gcgcttggtg ccgcccggga ccacgtcgag catcgcgagg tcgaggacgc cggggagcac
     3961 cggcaccgag gacgcgtcga tcctcgcgcc cacccccgag gccgcgagca tccgctgtag
     4021 gtggccgagc agcccaaacc ccgtcacgtc ggtcgccgcc acggcgccga cctcggccat
     4081 cgcgtcggcc gccgcatcgt tgggggtcgt catcagctcg acggcggccg ccagcagcgc
     4141 gggttcggcg accccgcgct tgtgtgcggt cgtcgcgatc ccgacgccga gcggcttggt
     4201 gaggaacagg tgcgcgtcgg gcggcaccgt cgagttccgg gtgatgcgtt cgggatcggc
     4261 gagcccgagc gcgaccatcc cgtacttcgg ctcggggtcg gtgatcgtgt ggccgcccag
     4321 gatcgcggcg cccgccttgc tcgcgacgtc gaggccgccc tgcagcactc ggccgagcat
     4381 ctcgatcggc aggtcgtcga ccggccatgc ggcgatgttc agcgccagga acggccgcgc
     4441 gcccatcgcg tacacgtcgg agagcgcatt ggtggccgcg atccttcccc agtcgtacgg
     4501 gtcgtcaacg atcggggtga agaagtcgag cgtcgcgacc agcgcctgcc cgtccggcag
     4561 ccgcaccacc gcggcgtcgt cccccgtgtc ggcggcaacg agcacgtcgg cccccacctc
     4621 gggaagggac agcgtgccga ggaccgtctc gagatcggcc ggcccgagct tgcatccgca
     4681 cccggccccg tggctaaagc tggtcaggcg tcgcgatccc tccatcgggc caggatacgg
     4741 ggacgagtga cgcgcggtgg cggctaggct gcgacccgtg cgaaccgcgg tcgtcgggca
     4801 cgtcgagtgg atcgagttcg cacacgtcga ggacgtacct cgggccggcg acatcgtgca
     4861 cgccaccgaa ccctgggagg agccgggggg cggcggcgcg gtctcagccg tgcagctcgc
     4921 ccggctggcc ggcgagtgca ccttctacac ggcgctcggg gatgacgaac gcggcacctg
     4981 gtcccggcgc cgcctggcgg ggctgggcgt caggctcgag gcggccaccc gagacgagcc
     5041 gacgcggcgt ggcgtggtgt tcgtcgacgc gaacggcgag cgaaccatca ccacgctcgg
     5101 ccggcgtctc gaacccacga ccgacgacga tctcccgtgg gacgagctcg aggacgtgga
     5161 cgccgtgtac ttcacggcgg gcgacccggc ggcgctccgc gaggcgcgcc gcgcgaaggt
     5221 gctggtcgcg acgagccgcg tctcggacct gctcgccgag gcggacgtgc ggctggacgc
     5281 ggtggtgggc agcggcaacg atccttcgga agcgttcgat cccgcgaggc tcacgcacac
     5341 gccgaacctc ctcgtccgaa ccgacggcgt tcgcggcggc cggtacgaga ccggcaacgg
     5401 tcggagcggc tcgtacgaac ccgtcccccc gcccgggccg gtcgtcgaca cctacggggc
     5461 cggcgactcg ttcgcggccg ggctgacgtt cgcgctcggg agcggcctgg gcgtcgagga
     5521 ggagctggcg ctcgcggcgc ggtgcggcgc ctggtgcgcg gcgggcaggg ggccgtacgg
     5581 caaccagctc tcggccgccg acctcgagtc gggctaaccg agcgtcgtcg agacctcgag
     5641 ccggtggtgt tcgcgcacgt cggcggacgc cacgaacgtc gcgggaccga gcagccggtc
     5701 gccgtcccgc agctcctcgc ggatc
//
LOCUS       DATBRK010000122         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1173150, whole genome shotgun sequence.
ACCESSION   DATBRK010000122 DATBRK010000000
VERSION     DATBRK010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173150"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..955)
                     /locus_tag="VIH70_03650"
     CDS             complement(176..955)
                     /locus_tag="VIH70_03650"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY8115488.1"
                     /translation="MGRLDGKVALITGGASGMGMVASKLFASEGASVVLTDVADDAGE
                     AVASDIEATGGQALYVHSDASDEADAEAMVKAAVDRFGGLHVLYNNAGVMLSRDGSVT
                     ETDASIWDTTLDINVKGVAFGCKYGIPAMIAAGGGSVINVASFVAWMGAATSQTAYTA
                     SKGAVLSMTREIAVEYARRGIRCNALCPGPIDTPLLAELLADPARRQRRFVHIPMGRL
                     GQAEELAKAALFLASDDSSFMTGASLIVDGGITAAYVTPED"
     gene            1069..>1987
                     /locus_tag="VIH70_03655"
     CDS             1069..>1987
                     /locus_tag="VIH70_03655"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8115489.1"
                     /translation="MTTELLVLGGERTAPADGATSDVIEPGTGEPMVEVSDAGAEDAR
                     RAVDIAHRAFEEGAWPRMSATERGRVLLRASALLRERLEEFAVVESRNAGKPINAARG
                     EIGTVANVLEYWGGAANKIFGETIPVSDAGLDVTLREPVGICALIVPWNFPLVIASWK
                     VGPSLACGNTIVVKPATYTPLSVLKLADLLVEAGLPPETMSVLPGPGSTVGNALVSDP
                     RVSKVGFTGSTAVGSRIMQRCAETITRVSLELGGKSANVVFADADMETCVERSMWSIF
                     DNTGQDCTSRARMFVERPVYDEFIERLAKR"
BASE COUNT          296 a          690 c          705 g          296 t
ORIGIN      
        1 cccgggcgga gcttccggaa cgcgaggatc cagatggcgg cgtagagcag cggcgtgaag
       61 tcgaagtcac cgcccgtcgg gatcgcgggg tgcccggtcg cgacggtcgc caggatgggg
      121 ctcatggctt cctatcgttc cctccgagcc gcgggtttag tcccatggcg caggttcagt
      181 cctcgggcgt cacgtaggca gccgtgatcc ccccgtccac gatcagcgag gcccccgtca
      241 tgaaggagga gtcgtcgctc gcgaggaaca gggccgcctt cgcgagctcc tccgcctgcc
      301 ccagccgccc catcgggatg tgcacgaacc tgcgctggcg ccgtgcggga tctgcgagca
      361 gctcggccag cagcggcgtg tcgatcgggc cggggcagag cgcgttgcac cggatccccc
      421 ggcgcgcgta ctcgaccgcg atctcccgcg tcatcgagag cacggcaccc ttcgaggcgg
      481 tgtaggcggt ctgcgaggtg gcggcgccca tccacgcgac gaacgacgcc acgttgatga
      541 ccgacccgcc cccggcggcg atcatcgccg gaatgccgta cttgcacccg aacgcgacgc
      601 ccttgacgtt gatgtcgagc gtggtgtccc agatcgacgc gtcggtctcg gtgaccgagc
      661 cgtcccggct caacatcacc ccggcgttgt tgtaaaggac gtgcaggccc ccgaaacggt
      721 ccacggccgc cttcaccatc gcctccgcgt cggcctcgtc cgacgcgtcc gagtggacgt
      781 acagcgcctg cccgccggtg gcctcgatgt cggaggccac cgcctcgccg gcatcgtcgg
      841 cgacgtcggt gaggacgacc gaggctccct cgctcgcgaa cagtttcgag gcgaccatcc
      901 ccatgccgct cgcgccgccg gtgatgagcg caaccttccc gtcgagccgt cccatcgtgc
      961 cctccctcgt gcgtcgcgcc gagcatacgc acccggttcg gtgaccggcc cttgcggcgg
     1021 ggccgcgcct atcatcgaac catcaggcga gcaacggagg aaccggccat gacgaccgaa
     1081 ctgctggtgc tgggcggcga gcggacggct cccgcggacg gagcgacgtc cgacgtgatc
     1141 gagccgggga cgggcgagcc catggtggag gtctcggacg cgggcgccga ggacgcgagg
     1201 cgcgccgtcg acatcgcgca tcgtgcgttc gaggagggcg cctggccgag gatgagcgcc
     1261 accgaacgcg gacgggtgct gctgcgcgcc tcggcgctgc tccgcgagcg gctcgaggag
     1321 ttcgcggtcg tcgagtcgcg gaacgcgggc aagccgatca acgccgcgag gggcgagatc
     1381 gggaccgtgg cgaacgtgct cgagtactgg ggcggggccg cgaacaagat cttcggggag
     1441 acgatccccg tgagcgacgc cggtctcgac gtgacccttc gcgagccggt cggcatctgc
     1501 gcgctgatcg tcccgtggaa cttcccgctg gtgatcgcct cctggaaggt cgggccctcg
     1561 ctcgcatgcg ggaacacgat cgtggtcaag ccggcgacct acacgccgct ctcggtgctg
     1621 aagctcgccg acctactcgt ggaggccggc ctgccccccg agacgatgag cgtgctcccg
     1681 gggcccgggt cgacggtcgg gaacgcgctc gtctccgatc cgcgggtctc gaaggtgggg
     1741 ttcacgggct ccaccgccgt cgggtcgcgg atcatgcagc ggtgcgccga gacgatcacg
     1801 cgtgtctcac tggagctcgg cggcaagtcg gccaacgtgg tgttcgccga cgccgacatg
     1861 gagacgtgcg tcgagcgttc gatgtggtcg atcttcgaca acaccggcca ggactgcacg
     1921 tcgcgggcgc ggatgttcgt cgagcgaccc gtctacgacg agttcatcga acgcctcgcc
     1981 aagcgga
//
LOCUS       DATBRK010000123         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_161899, whole genome shotgun sequence.
ACCESSION   DATBRK010000123 DATBRK010000000
VERSION     DATBRK010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161899"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VIH70_03660"
     CDS             complement(<1..386)
                     /locus_tag="VIH70_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY8115490.1"
                     /translation="MSAGVHETDEVRVDETVESDGTARSRRRWWTVALVLTPLVLFAL
                     LLATGLGRDPRELPSELIGQPAPRFSLPRLDAGGTIDLGDLHGQVVVVNFWASWCVPC
                     REEHDALAAAWGRYRDRGVVVLGVSF"
     gene            complement(383..>2228)
                     /locus_tag="VIH70_03665"
     CDS             complement(383..>2228)
                     /locus_tag="VIH70_03665"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEY8115491.1"
                     /translation="LAALLANDFSLRYVASNSSRATPTFFKMLALWSADEGSLLLWNL
                     VLGGYLAAVAFRFRRRRPETFPWALAVMFGISAFYLILVLGPTTPFATLATAPVDGRG
                     PLPLLQNHPLMAAHPPFLYLGFIGFTVPFAFAIAALITGRVSDAWMRITRRWTISAWI
                     FLTIGLVLGALWSYSVLGWGGYWAWDPVENVALLPWLVGTAFLHSVMVQERRGMLKVW
                     NLSLIVGTFALTVLGTFLTRGSILASVHAFAESIVGPLYLAFLTLVLLGGFGLIAWRR
                     ESLRAEATFDTPLSRESVFLGNNVLLLVLTFTVLVGTIYPLLAEAVTGGKVSVGSPFF
                     NRNAAPVALLLLFLMGIGPLLPWRAASPSAVLRRLQVPTWAAAVTAAVLALTGAGVMA
                     VIVFGLAAFVAAATVAEMVRGVRAQRRAHGISVVAAIGSAVARNHRLYGGLVVHLGLI
                     VAVVGVSWAALADRSSEATIARGETVHAEGYDLRFDGLTTREEPQRRVLVAELTVLDP
                     ETGDEVVRLRPSLNLYPSASEPIGTPSIRTGTPFNGMVDLYASLVAVEGDTRASFRFF
                     VNPGIGLLWLGGAVMALGGVVAAWPTRPRAASPRGPQRPAERREEVTV"
BASE COUNT          393 a          823 c          780 g          232 t
ORIGIN      
        1 tcgaacgaca cgccgaggac gacgacgcct cggtctcgat accggcccca ggcggcggcg
       61 agcgcgtcgt gctcctcccg gcacggcacg caccacgacg cccagaagtt gacgacgacc
      121 acctgcccgt gcaggtcacc gaggtcgatc gtgcctcccg catcgagcct cggcagcgag
      181 aacctcggcg ccggctggcc gatcagctcg ctggggagct cacgcgggtc ccggccgaga
      241 cccgtggcga gcagcagcgc gaacagcacc agcggagtga gcaccaacgc gacggtccac
      301 caccgacgcc gcgagcgcgc cgtgccgtcc gattccacgg tctcgtcgac ccgaacctcg
      361 tccgtctcgt ggacgccggc gctcacaccg tcacctcctc gcgacgctcg gccgggcgtt
      421 gggggccgcg tgggctcgcc gcccgcggcc gggtcggcca ggccgcgacg acgccgccga
      481 gcgccatcac cgcgccgccg agccacagca gcccgatgcc cgggttcacg aagaacctga
      541 acgaggcgcg cgtgtcgccc tcgaccgcga cgagcgacgc gtacaggtcg accatgccgt
      601 tgaacggcgt gccggtcctg atcgacggcg tcccgatcgg ctcggacgcc gacgggtaca
      661 ggttcaggga cggccggagc ctgactacct cgtcccccgt ctcggggtcg aggaccgtga
      721 gctcggcgac caggacccgt cgctggggct cctcgcgggt cgtgagcccg tcgaaccgga
      781 ggtcgtagcc ctccgcgtgc acggtctcgc cccgggcgat cgtggcctcc gaggagcgat
      841 ccgccagcgc cgcccaggac acgcccacca ccgcgacgat cagcccgagg tgcaccacga
      901 gcccgccgta gaggcggtgg ttccgcgcga ccgcgctccc gatcgccgca acgaccgaga
      961 tcccgtgcgc gcgacgctgc gcccggacgc cgcggaccat ctcggcgacc gtcgccgccg
     1021 cgacgaacgc cgccagtccg aacacgatca ccgccatcac gcccgctccc gtcagcgcga
     1081 ggacggccgc ggtcaccgcg gcggcccacg tcggcacctg cagccggcgc aggaccgcgg
     1141 agggagacgc cgcccgccac ggcagcagcg gcccgatccc catcaggaac aggagcagca
     1201 acgcgacggg cgccgcgttc cggttgaaga acggcgagcc gaccgagacc ttgccgccgg
     1261 tgaccgcctc ggcgagcagc gggtagatcg tgccgaccag cacggtgaac gtgagcacca
     1321 acaacaggac gttgttgccg aggaagacgc tctcgcggga cagcggcgtg tcgaaggtcg
     1381 cctcggctcg cagcgactcg cgacgccacg cgatcagccc gaaccctccc agcagcacca
     1441 gcgtgaggaa cgcgaggtac agcgggccga cgatcgactc ggcgaaggca tggaccgacg
     1501 cgaggatgct cccccgtgtc aggaacgtcc cgaggaccgt cagcgcgaac gtcccgacga
     1561 tcagggacag gttccagacc ttcagcatcc cccggcgctc ctgcaccatc accgagtgca
     1621 ggaaggcggt gcccacgagc cacggcagga gcgcgacgtt ctcgaccggg tcccacgccc
     1681 agtacccgcc ccagccgagg acgctgtagg accacagggc gccgagcacc aggccgatcg
     1741 tgaggaagat ccacgcggag atcgtccacc ggcgcgtgat gcgcatccag gcgtcgctga
     1801 cccgcccggt gatcagcgcg gcgatcgcga acgcgaacgg cacggtgaac ccgatgaacc
     1861 cgaggtacag gaacggggga tgcgcggcca tcagcgggtg gttctgcagc aacgggagcg
     1921 gaccccgacc gtcgaccggg gcggtggcga gcgtcgcgaa cggcgtcgtc ggcccgagca
     1981 cgaggatcag gtagaaggcc gagatgccga acatgaccgc gagcgcccac gggaaggtct
     2041 cggggcgccg gcggcggaac cggaaggcga cggccgccag gtagccaccg aggaccaggt
     2101 tccacagcag cagcgacccc tcgtcggcgg accagagcgc gagcatcttg aagaaggtgg
     2161 gggtcgcgcg gctggagttc gacgcgacgt agcggagcga gaagtcgttc gcgagcagcg
     2221 ccgcgagc
//
LOCUS       DATBRK010000124         3873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2327762, whole genome shotgun sequence.
ACCESSION   DATBRK010000124 DATBRK010000000
VERSION     DATBRK010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2327762"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..1427
                     /locus_tag="VIH70_03670"
     CDS             453..1427
                     /locus_tag="VIH70_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY8115492.1"
                     /translation="MTPFRRHLGRRVAGVGVAATLVVLGLEAPAFAVPPSITSFSPTS
                     GPAGCVVVITGTDFDNPVVTSVDIGGTPVTAFKIVSATKIWATVVGTTSGPIHVTNAN
                     GTANSPTEFTNTNPGACSPTITSLTPCSGSAGTTVVIVGKNLLKYSGTTTTAAMGGDV
                     WFAPYAATATHTVPNVDTPTSLSVVVPSDAADGPIRVSTFNEVIGEGAVLSGTAFQVP
                     PPDVTCAPADHARSITLNLRRHLVARGKVSVGDGFTDCAVSVPVKVQRRRAGGGWRTV
                     GRTSTTDTGAYRKRIKDRPGRYRSVAPKINQFTDLCFRAVSSVRRHNL"
     gene            complement(1500..1796)
                     /locus_tag="VIH70_03675"
     CDS             complement(1500..1796)
                     /locus_tag="VIH70_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEY8115493.1"
                     /translation="MACSRPAFVPRRFSHYQYDRGITLREAARLQSFPNEFEFVRPRL
                     QVARQIGWLAPSRCRSVGHSDLPSQTMPLPKSSSSEPITVLGKAGVSNLRVAFR"
     gene            2389..3003
                     /locus_tag="VIH70_03680"
     CDS             2389..3003
                     /locus_tag="VIH70_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8115494.1"
                     /translation="MGKVRTGLSVSLDGFISGPNDGPDTPMGEGGELLLAWYASGDTE
                     YEMPGTGMVFKVAAATADYLRETQERTGALVFGRRTFEFTNGWGGKHPLDVPVFILSG
                     SIPQEWVYEGSPFTFVTDGLESAVKQAKAIAGDKDVGVGAASVVQQCIKAGLLDEIHL
                     DLVHVLLGGGVSLFDRLGTGPIDLERTRVIEGTGVTHLTFTIVK"
     gene            3675..>3873
                     /locus_tag="VIH70_03685"
     CDS             3675..>3873
                     /locus_tag="VIH70_03685"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8115495.1"
                     /translation="MTGDPIVELDADFSAEDGRTTPWATALELLEKAEVFWLSTVRAD
                     GRPHVTPLVAVWLDGALYFCTG"
BASE COUNT          712 a         1186 c         1263 g          712 t
ORIGIN      
        1 cgaggtgtca tggcatttcg agcaggtctc gccgttgaac gccgaacccg tgccggaggt
       61 cacccgcgat ctcatccgca gtatgccttt tcacaccaac aggccattcg tgcgccggcg
      121 attcagctgt gagaatggca cagcggcagc atgcggtccc gcgaccacaa actcgcggga
      181 ttcgagctgg atccttctct gggcacgaga ttcatgagcg agctgggttg cactcgagcc
      241 ttctgctccc tttgggatag gtcatacttg gctgagaatc tgcgtagacg ctatccggat
      301 cgcattctga tccggcgtcc agtccctcgt ccgctaggca accttcttgt gcgcccctcg
      361 ttatcaccag tgcggagctt cggaagacga ccggcgcggg caacgcaatt ggagccaagc
      421 gggtctcgtt tgcggaacgt gaggacgaag ccatgacacc gttcaggcga cacctcggtc
      481 gacgcgtagc gggagtcggg gttgcggcga ccttggtcgt gctcggcctc gaggccccgg
      541 cattcgcggt tcccccgagc atcacctcgt tctcgccgac cagtggcccg gcaggctgcg
      601 tggtggtgat cacaggaacg gacttcgaca acccagtggt gacgagcgtc gatatcgggg
      661 gcacgccggt gaccgcgttc aagatcgttt cggcgaccaa gatctgggcg acggtcgtcg
      721 ggacgacaag cgggccgatc catgtcacaa acgcgaacgg tacggcgaac agcccaaccg
      781 agttcaccaa caccaacccg ggggcctgct ctccaaccat cacgtcgttg acgccatgtt
      841 cgggatcggc cgggacgacc gtagtgatcg tggggaagaa tctcctcaag tactccggca
      901 cgaccacaac agccgccatg gggggcgacg tgtggttcgc tccgtatgca gccacggcga
      961 cgcataccgt tcccaatgtt gacaccccga cttcgttgag tgtggtcgta ccgtcggatg
     1021 ccgcggacgg accgattcgg gtgagcacgt tcaacgaggt cattggtgag ggagccgttc
     1081 tcagtggtac cgccttccag gtgccgccac cagacgtcac gtgcgcgccc gccgaccacg
     1141 cgaggagcat cacgctgaac ctacggagac acctcgtcgc tcgagggaag gtcagtgtgg
     1201 gcgacgggtt caccgactgc gcggtgagtg ttccggtcaa ggtccagcgc cgtcgtgctg
     1261 gtggcgggtg gaggacggtg gggaggacct cgacgactga caccggggcc tacaggaagc
     1321 ggatcaagga tcgcccaggt agataccggt ccgtggctcc gaagatcaac cagttcactg
     1381 acctctgttt ccgagccgtc tcatccgttc gaaggcacaa cctctaggct gttctggagt
     1441 gccgtgcgcc aggcacgacc cttgctcgtc tgcacgatct tcggagccct cgacgccttt
     1501 cagcgaaacg ccacccgcaa gttgctgacc cctgccttcc cgaggacggt tatgggctcc
     1561 gacgacgagc tcttcggaag gggcatcgtc tgcgacggca gatccgagtg cccgaccgat
     1621 ctgcaccgcg aaggcgcgag ccatccaatc tggcgtgcaa cctggagccg aggacggacg
     1681 aactcgaact cgttggggaa cgactgcaac cgagccgcct ctcgaagtgt gatgcctcgg
     1741 tcgtattggt aatgggagaa gcgccgcggg acaaatgcgg gacgagaaca agccatatcg
     1801 gcccgccgca accggacagg gcccctgacc tgcgtttttc ctggtagtcc caacggggtt
     1861 cgaacccgtg tctccacctt gagagtgttc tcggggccga tctcgctacc tgctcggttg
     1921 gccgaatctg ggcgatgacc tgcgaattcg atgctcgaaa ccctccgacc acttcttgac
     1981 gtctcctggt cgttcgcggg acgaatgcgg gacgagtcgg ggagtgcaag ggccaagcgg
     2041 aggatgggtc atcgactcat ttggcgatcg agggtagcca gcgaattccg cgacgccgcc
     2101 gccagcctcc cggagtagcg tcctcacccg ctccgaggcc gaccggggcc gtgttgattg
     2161 gccgactgtc ccagcgggcc gacgctgagt gatcgaacag ctcattcgct ccgccaatgc
     2221 tcaccatcca tccactggcg agctcctgat cgactcgagg aggaggatga gcccgcacgg
     2281 ttttggacct gtttgatgga ttgcggcagg cgacctaacc ttcaccccta cattcgtcgc
     2341 ccatgacgcc gccgcccgaa tcgtgggcat tgcaaagggg gcgcgaccat ggggaaggtg
     2401 cgcacagggc tctcagtctc gctcgacggg ttcatcagtg gaccgaacga tggccccgat
     2461 acgcccatgg gcgagggcgg agagttgctc ttggcgtggt acgcgtccgg cgacactgaa
     2521 tacgagatgc ctggtactgg catggttttc aaggtcgccg ctgcgaccgc cgactacctg
     2581 cgcgaaacgc aggagaggac gggggccttg gtgttcggtc gacggacgtt cgagttcacc
     2641 aatgggtggg gtggcaagca tcccctcgac gttccggtct tcatcctttc tggctcgatc
     2701 ccgcaggagt gggtctacga aggatcgcca ttcacgttcg taaccgacgg cctcgaaagc
     2761 gcggtgaagc aagcgaaggc tattgctggc gacaaggacg tcggcgtggg cgctgcaagc
     2821 gtcgttcagc agtgcatcaa agccgggctg ctcgacgaga ttcacctgga cctagtgcac
     2881 gtccttctcg gcggaggtgt ctcgttgttc gatcgtctag ggacggggcc gatcgacttg
     2941 gagagaacaa gggtaatcga gggcacgggc gtcacacatc tcaccttcac catcgtgaag
     3001 taaggccgag ctcctaatcg acctcgagga gatgagccgg cacggctcag cctggtcgag
     3061 gcctccgacg aacgctcacg tagctcaagc gcgggggcta gtgccttcac ccgggcgtgg
     3121 ggattcaaac cgggggtcga gaccgggaag ctgggcgggc agaagatacg caggcctgtg
     3181 cgttcggcga gcaaggggac tacgagctcg gcgtgcttct cgaggtcgag gcggggcgag
     3241 gtgcgcgggg caatccccgt ctacagagat ctcgttcgga cgtcgctcgt cgttggcaac
     3301 gtggtcgttc cagctgaccg gccgtgacgt atcgcggtcg gccgcgggac aaatgcggga
     3361 cgagaacaag ccagatcggc cgccgcaacc ggacagggcc cctgacctgc gtttttcctg
     3421 gtagtcccaa cggggttcga acccgtgtct ccaccttgag agggttctcg gggtagattc
     3481 caccacctgc agcgatgcca tgagatgctc gtctacctgc gggtttgctt ctcttcgcac
     3541 ccggtcctct tgcgtcgtcg cccggtacgt cgcggggcca atgcggggcc aacatgttga
     3601 tgccgtctta caacgaaggc tcaggctcac tctcgggctg aggatcatcc cgacgccaca
     3661 cggaggagga ggccatgaca ggtgatccca tcgtggaact ggacgcggac ttcagcgctg
     3721 aagacgggcg cacgacgccg tgggcaacag cactcgagct gctggagaag gccgaggtgt
     3781 tctggctttc aacggtgcgc gcggacggac gaccgcacgt cacaccgctt gtcgcggtct
     3841 ggctcgatgg cgcgctgtac ttctgcaccg gcg
//
LOCUS       DATBRK010000125         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2472666, whole genome shotgun sequence.
ACCESSION   DATBRK010000125 DATBRK010000000
VERSION     DATBRK010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472666"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..>1929
                     /locus_tag="VIH70_03690"
     CDS             13..>1929
                     /locus_tag="VIH70_03690"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY8115496.1"
                     /translation="MRGTMTGKILEAHLAEGRLVPGEEVALRIDQTLLQDATGTMACM
                     EFEALGLEEVTTNPAVQYVDHNMLQFDHRNADDHLFLQTFAARYGIKFSRAGNGICHQ
                     VHTERFARPGATLLGADSHTPTSGACGSIAIGAGGLEVALAMAGHPFRMPIPVVVAVH
                     LDGELPPWVASKDLILSLIRHYTLKGGLNRIFEFTGPGVANLPVTRRATVCNMITELG
                     GTTAIFPSDEQTRRFLRRQHREDQWVELGPDPDASYDEEVHLDLSSLEPLIAKPSQPD
                     NVVPVEQVAGTPVFQVCFGSSVNSWYEDLAIPAAVLDGHHLPPTTVGTVSPGSRQILT
                     MITTSGILEKLEWAGARILEPACGPCVGIGQAPPTGKASVRTFNRNFKGRSGTVDDQV
                     YLASPATTAATGLHGRITDPRTLGEPPVIDDPEPVVDDFMILEPAQPGARERIEVIRG
                     PNIKNPPIPPPLPDDMTGQVLIVLPDNISTGSMAPDGAIVMADRSNVAAIAEYTFMKE
                     DPAFVRRAKAWQGGFIVAGENYGQGSSREHAALAPLQLGVRGVLAQGFARIHRRNLVA
                     QGLVPLYIGADVHDRLEVGDRLAIRGLKVAVASGADQVGVEVDRKEPFRARLDLSPRE
                     RRIVLAGGVLNQLST"
BASE COUNT          304 a          748 c          618 g          259 t
ORIGIN      
        1 caggaggcca gcatgcgggg caccatgacc ggcaagatcc tcgaggcgca cctggccgag
       61 gggcgccttg ttccgggtga ggaggtcgcc ctgcggatcg accagacgct cctgcaggac
      121 gcgaccggca cgatggcctg catggagttc gaggcgctcg gcctcgagga ggtgacgacc
      181 aaccccgccg tgcagtacgt cgaccacaac atgctgcaat tcgaccaccg caacgccgac
      241 gaccacctgt tcctgcagac cttcgcggcc aggtacggca tcaagttctc gcgggcgggc
      301 aacggcatct gccaccaggt ccacaccgaa cggttcgcgc ggcccggcgc caccctgctc
      361 ggcgccgact cgcacacgcc aacctcgggc gcgtgcgggt cgatcgcgat cggcgccggt
      421 gggctggagg tcgcgctcgc gatggccggc cacccgttcc ggatgccgat cccggtcgtc
      481 gtcgccgtcc acctcgacgg tgagctgccg ccctgggtcg cctccaagga cctgatcctg
      541 tcgctcatcc gccactacac cctcaagggc ggcctcaacc gcatcttcga gttcaccggc
      601 ccgggcgtcg cgaacctgcc ggtcacgcgg cgggcgacgg tctgcaacat gatcaccgag
      661 ctcggtggga cgaccgcgat cttcccgtcc gacgagcaga cccgccgctt cctgcgccgc
      721 cagcaccgcg aggaccagtg ggtcgagctc ggccccgacc ccgacgcctc ctacgacgag
      781 gaggtccacc tcgacctgtc cagcctcgag ccgctcatcg ccaagccgtc ccagcccgac
      841 aacgtcgtcc cggtcgaaca ggtcgcgggc acgccggtct tccaggtctg cttcggctcg
      901 agcgtgaact cctggtacga agacctcgcg atcccggccg cggtcctgga cggccaccac
      961 ctgccgccga ccacggtcgg cacggtctca ccgggcagcc ggcagatcct caccatgatc
     1021 acgacctcgg ggatcctgga gaagctcgag tgggccggtg cgcggatcct cgagccggcc
     1081 tgcgggccgt gcgtcggcat cggccaggca cccccgaccg gcaaggcgag cgtgcgcacc
     1141 ttcaaccgca acttcaaggg ccgctccggc acggtcgatg accaggtcta cctcgcctcc
     1201 ccggcgacca cggcggccac cggcctgcac ggcaggatca ccgacccacg caccctcggc
     1261 gagccgccgg tgatcgacga tcccgagccg gtcgtcgacg acttcatgat cctcgagccg
     1321 gcccaacccg gcgcgcgcga gcggatcgag gtcatccgcg gccccaacat caagaacccg
     1381 ccgatccccc cgccactgcc cgacgacatg accgggcagg tcctcatcgt cctgcccgac
     1441 aacatctcaa ccgggtcgat ggcccccgac ggggcgatcg tcatggccga ccggtccaac
     1501 gtggccgcca tcgccgagta caccttcatg aaggaagatc ccgccttcgt gcggcgggcc
     1561 aaggcgtggc aaggcggctt catcgtggcc ggcgagaact acggccaggg ctcaagccgc
     1621 gagcacgccg ccctcgcccc cctgcagctc ggcgtccgcg gcgtgctcgc ccaggggttc
     1681 gcccgcattc accggcgcaa cctggtcgcg caagggctcg tcccgctcta catcggcgcc
     1741 gacgtccacg accggctgga ggtgggcgac cggctggcga tccgcgggct caaggtcgcg
     1801 gtcgcaagcg gtgcggacca ggtcggggtc gaggtcgaca ggaaggagcc gttccgcgcg
     1861 cggctcgacc tctccccccg cgaacgccgg atcgtcctcg ccggcggcgt gctgaaccag
     1921 ctctccacc
//
LOCUS       DATBRK010000126        12660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_453803, whole genome shotgun sequence.
ACCESSION   DATBRK010000126 DATBRK010000000
VERSION     DATBRK010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453803"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /gene="infC"
                     /locus_tag="VIH70_03695"
     CDS             <1..550
                     /gene="infC"
                     /locus_tag="VIH70_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY8115497.1"
                     /translation="LRQAQDLDLDLVEVAPQANPPVARIMDYGKFKYERDIRQKEARK
                     KQARIEVKEIKFRPKIDPHDYATKKGHVERFLRTGARVKVTIMFRGREMARTELGRKI
                     LDRLVADLADQAVVEAHPKQDGRNMVMVLAPSKKPPEPKSTEPRNRQEPEAAAPRAAA
                     EPVQEQAPDEVVAAPAEAPPET"
     gene            607..798
                     /gene="rpmI"
                     /locus_tag="VIH70_03700"
     CDS             607..798
                     /gene="rpmI"
                     /locus_tag="VIH70_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY8115498.1"
                     /translation="MPKQKTHRATAKRFRITRTGKVLHRKATGNHFLRKKRGSRRRRV
                     EGMTQVTAERTNIRRLLGK"
     gene            804..1163
                     /gene="rplT"
                     /locus_tag="VIH70_03705"
     CDS             804..1163
                     /gene="rplT"
                     /locus_tag="VIH70_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY8115499.1"
                     /translation="MARVKRSVHAKKKRREVLRKAKGYFGSRSKRYRVAKEQILHSGQ
                     YAYRDRRDRKAQFRRLWIARIGAGARQHGLSYSRFVHGLSKAGIEVDRKMLADMAVHD
                     PAAFGQLVEAAKDALAA"
     gene            1184..2632
                     /locus_tag="VIH70_03710"
     CDS             1184..2632
                     /locus_tag="VIH70_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY8115500.1"
                     /translation="MITSVRNPKVAAALKLHKRAFRERERAFLVEGVQALGEALSMPR
                     GGISALFHTEPSHPLVERARAAGVAATHVSDDVMARLAGTVTPQGFVGVAGYLDVPFD
                     HLPDEPAGVALLHEVRDPGNAGTVLRSADAVGADAVVFSSSSVDVYNAKTVRASAGSL
                     FHLPVVRGSDTASAVAALRARGMRILAMDADGEGDLYEEDLSDPVAFVFGNEAWGLPK
                     EISALADATVRVPITGRAESLNLAAAATVCLFEWERQRREGRRAVLETIIAAAAHDIR
                     SPLTALKGIGHALATRWEQMRPEDRELMLRAIQFDTDRLNAILRQLVDAARVAAGALD
                     LFPQRTSVSHVVEEIATSLARDPDHVRVEWAAGDDLNVFVDPERLRLVVEAFVESLVW
                     WASEGPVRVEGRVRDGRLSVEARRAETELTQEQAERLFRPRAPGTGSGSKIGLFVALG
                     VAEAQGGGVSARVADGTLIFALDLPVPSEPDA"
     gene            2697..3743
                     /gene="pheS"
                     /locus_tag="VIH70_03715"
     CDS             2697..3743
                     /gene="pheS"
                     /locus_tag="VIH70_03715"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY8115501.1"
                     /translation="MDPSEAARILEQELERGRELIDAARTVEELDEAKTAVLGRKAPI
                     ASVQSSLGALPADARRDLGRRTNEAFAELRTELDARRAELEEGAEDELLRADAVDVTL
                     PGRRPRPGSLHPLSLVQDRIVEIFTRMGYRVAVGPEIEDEWHNFEALNIPPDHPARTM
                     KDSLYVDADGLLLRTETSSVQIRTMESQEPPISVIVPGRTYRREAVDATHLSVFHQVE
                     GLVVDEGISFADLRGTLEAFAREMFGEDVRVRLTPDYFPFVEPGCQAAVSCFVCGGVG
                     CRVCGNGWLELLGAGMVHPKVLENSGIDSERYTGFAFGMGIERVAMVRYGVNDMRLLV
                     EGDVRFLERFEGAA"
     gene            3740..6115
                     /gene="pheT"
                     /locus_tag="VIH70_03720"
     CDS             3740..6115
                     /gene="pheT"
                     /locus_tag="VIH70_03720"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY8115502.1"
                     /translation="MRVVLSWLREMCPTERSAEDLAELLTAKGAEVEAIDYPWERVSG
                     VVVARVLEVRDHPNSDTLCLARVQTGSGELEVVVGVRNMAPGDLVPLAPPGASVLTLP
                     EPLEAREIRGVVSQGMLCAPDELGISASHEGILVLPGDLQPGTDVAEAFGLTDAVLDI
                     EVTPNRPDFLSVLGIAREVSAATGTPLSPPDTDVKEDAEPAEGVATLEVADLERCPRY
                     LARIVRDVRHVPSPIAAQARLFAAGMRPISAVVDATNYAMLEIGQPLHPFDLALLKGP
                     GIVVRRAVAGETLVTLDGVERAFTEDDLLICDAERPVGVAGVMGGELAEVSEQTSDLL
                     LEAAWFERGGVQRTRRRIGLSTEASIRFERGVDPEAAPTGADRACRLMSEWCGARVLR
                     GLVEVGGPPPRRRIELRAPRASALIGYAVKNVDAVEVFERLGIGTETVDEDTILVEIP
                     GYRVDLEREVDLIEEVVRVQGYERVGSTLPPIARPGGLPAAYGFRTRVRDALRRAGLR
                     EVRQIPFVSEADLRMVGDDDAIRVTNPLQADDGWLRTRLTPGMLKVVRHNAYRHVRSV
                     AVFEVDTVFRMRDGRPDERPKAAFALNGNADPGWARDDRPFDVFDAKGVVEAVMAELG
                     ISWTLGDPVGRPFHPGRSGAIMVGEERIGVLGEIHPRVADRLGLTGRVAVAELEVEAL
                     MRLARPVSGVEDVPRFPPVRRDLAFTVDASIPAGRVRSALEDAAGEIVGSVLLFDVFE
                     GPPLPEGTKSLAYSVDFRVPDRTLTDEEVDGAVDAIVQRLSRDVGARLRSG"
     gene            6207..7127
                     /gene="argF"
                     /locus_tag="VIH70_03725"
     CDS             6207..7127
                     /gene="argF"
                     /locus_tag="VIH70_03725"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY8115503.1"
                     /translation="MERDLLSIDDLSPRELGQLLELSAKVKAQPADYADRLAGRSVAM
                     IFEKPSTRTRVSFEIAVASTGGHPVPLSSADLQLGRGETIEDTGRVLSRYVQAIVLRT
                     FEQERLEVLASAASIPVVNALSDFEHPCQCLADLLTIKEVKGELAGTIVTYLGDGNNV
                     THSLLFGGAKAGMHVRAGTPPGFEPIPQVVQRASDIASQTGGSVTITHDPIEASRGAD
                     VLYTDVWASMGQEAEADERALVFPAYALDAEKVAVAAPDVTVMHCLPAHRGQEIAADV
                     IDGPHSVVWDQAENRMHAQKALMLWLLGLA"
     gene            7150..8871
                     /gene="polX"
                     /locus_tag="VIH70_03730"
     CDS             7150..8871
                     /gene="polX"
                     /locus_tag="VIH70_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEY8115504.1"
                     /translation="MARLNDTVEAALIEYADLLSILTEDPYKPRSYEKAARAVGGYPD
                     DLATMDLKEILGIPSVGKSIGEKIDEFVRTGSFEALDELRAQIPPGVREMTAIPGLGP
                     KKAILLYRDLGIADVEQLAQAVEEGRLAEVKGFGAKTEENIRRGIERMTQSTGRVLVS
                     VAMDLADYFIERLGSRKDVRRIEFAGSLRRMAETIGDLDLLVASDDPAGVMEAFTTIG
                     MTERVLARGETKSSILTRKGVQVDLRVVPLEAWGAAMIYFTGSKAHNIRIREMAVRKG
                     LKLNEYGLYRAKSGKLIVAETEEEVYEKLSLSWIPPTLREDRGEVEAALEGTLPDVLV
                     RGQLRGDLHTHTNLTDGLASLEQMLAAASELKYKYYAVTDHAPNLFMQRMTDEKMLAQ
                     REQLRALQSAYPKMTLLHGTELNIDAEGNVDWDGDFLEGFDVCVASVHSLFNQPKHEM
                     TRRIVRAMENPNVHVIGHPTGRMINKRQPVEVDLEEVFKAAARTGTALEVNGYPDRLD
                     LRDEHILWARRHGVKFSVDTDSHATTHLEHMRYGVATAQRGWLTERDVINAWPLSKLR
                     AFLRKGR"
     gene            8868..9545
                     /locus_tag="VIH70_03735"
     CDS             8868..9545
                     /locus_tag="VIH70_03735"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY8115505.1"
                     /translation="MRRTPGGATGFGRRLPRSFFARPSTVVAPELLGRVLVRIRPDGT
                     RLAGRLVEVEAYEQGDPASHSFRGRPTPRTEVMFGPPGRLYVYFTYGQHFCSNVVTGP
                     EGYGSAVLLRAAEPLDGFETMAANRGTDAVRLLCSGPARLTRALEIDRADNGTDVVKD
                     PSLFLLAGSPVGPRATGRSTRVGVNVGVEHVWRFFERGNAFVSPGKPSVPDVDGAQRR
                     ATGSRRS"
     gene            complement(9523..12003)
                     /locus_tag="VIH70_03740"
     CDS             complement(9523..12003)
                     /locus_tag="VIH70_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8115506.1"
                     /translation="MAPRSRTLVVGVFVFALAASACDLPSLHEARKSADALPETSFIY
                     SADGALITTLHAGENRVVVRSRKIPDVMRDAVIASEDQRFYDHSGLDVRALLRAAYVD
                     ATTGRVVEGGSTITQQLVKKLYVGDEQTVGRKIREAYLAWQLEQRLSKDRILTRYLNT
                     VYFGNGAYGILAASETYFGVEPLELTLSQAATLAGLIAAPVDYDPVRHPGRSERRRNH
                     VLTQMRGLGMIDEAEFEKARGSPIALDLAPEDAMHYPAPWFVDYVKEWFLSNPRFGET
                     PQDRYDLLFEGGLRIVTTVDLRLQRAAERAIGSVLTEPADPYGALTAIDPRTGYVRAM
                     VGGRDYWNEDDDFARINLATGGVTGRQAGSAFKPFALVAALEHGVPRTQPLNGSTAHI
                     LLQDGSYWDPHNAEGSGYGTISLESATVNSVNVAYANLLSVIGDGDPYAGAAALVEAA
                     VRMGIRCCPRTTEPNGPLAAVPAAVLGVNEVSTLEMASAFGTLANLGRHVQPTPVISV
                     TSSDGDVIYQAPSRAKQAVEPAIAAEAVDILKGVVSSGTGVGANIGRPQFGKTGTAQN
                     ASDAWFVGAVPQLVTAVWVGFPQGQIPMCCGNVRISTVYGGTWPASIWHAFMLAATRG
                     LPVKEFGTSPNVAFVTLRVDITQGCLANPYTPPGDVDVLRFPSGAEPTLEVCSEPSSY
                     QLPVVPSVIGLERQEAISALHNAGFMVATVLARSDRQPGTVIAQDPGGGSRLILTGTV
                     TITVARGAPEPSPAPDLAPAPNVVGMPRGAAEAEIQQAGFVASVSTGECDPDDPSCDD
                     RPGVVWSQSPDAGAQRERGSTITIFVNP"
     gene            12056..>12660
                     /gene="tyrS"
                     /locus_tag="VIH70_03745"
     CDS             12056..>12660
                     /gene="tyrS"
                     /locus_tag="VIH70_03745"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY8115507.1"
                     /translation="MGPKDLPTLLAGSAEVVPAEDLEHKLSLGRPLRVKLGLDPTAPV
                     VTLGWAVVLHKLRQFQDAGHTAVLIVGDFTAQVGDPSGKTETRPRLSEEEVRGHAERL
                     LDQFWKILDPERTEVRYNAEWLGPMGMEAILRLTASTTVARMLERDDFANRFAAREPI
                     SISELLYPLMQAYDSVAVAADVELGGTDQLYNLLMGREVMEA"
BASE COUNT         1911 a         4308 c         4608 g         1833 t
ORIGIN      
        1 gctccgtcag gcacaggatc tggatctgga tctcgtggag gtggcgccgc aggcgaaccc
       61 tcccgtggcc aggatcatgg actacgggaa gttcaagtac gagcgcgaca tccggcagaa
      121 ggaagcccgc aagaagcagg cgcggatcga ggtcaaggag atcaagttcc gtccgaagat
      181 cgatccgcac gactacgcaa ccaagaaggg ccacgtcgag cggttcctcc ggacgggcgc
      241 gcgggtcaag gtgaccatca tgttccgagg gcgcgagatg gcccgcacgg agctcggccg
      301 caagatcctg gaccggctgg tcgcggacct cgcggatcag gcggtcgtcg aggcccatcc
      361 caagcaggac ggacgaaaca tggtgatggt cctcgcgccg tcgaagaagc ccccagaacc
      421 gaagagcacc gagccgagga accggcagga gccggaggct gccgctcccc gggcggcggc
      481 cgagcccgtc caggagcagg cgccggacga ggtggtggcg gccccggcgg aggcgccgcc
      541 ggagacgtaa cgggaaacgc acgaggagtc gcacgcggca cgggttcgac cagaggaaga
      601 agagcgatgc ccaaacagaa gacgcaccga gcgacggcca agcggttccg catcacgcgg
      661 accggcaagg tccttcatcg gaaagcgacc ggcaaccatt tcctgcggaa gaagcgcggg
      721 agccgccgac gccgcgtgga gggcatgacc caggtcacgg ccgagcgcac caacatccgt
      781 cggctcctcg ggaagtaggg acgatggcac gggtcaaacg atcggtccac gcgaagaaga
      841 agcgacgtga ggtgctccgc aaggcgaagg ggtacttcgg gtcgcgatcg aagcgctacc
      901 gcgtggccaa ggaacagatc ctccattcgg ggcagtacgc ctaccgcgac cgacgagacc
      961 gcaaggcgca gttccgccgc ctgtggatcg cccggatagg ggcgggcgct cgccagcacg
     1021 gtctgtccta ctcgaggttc gtgcacggcc tgagcaaggc cgggatcgag gtcgaccgca
     1081 agatgttggc cgacatggcc gtgcacgacc cggccgcgtt cggccagctc gtcgaggccg
     1141 ccaaggacgc gctcgcggcc tagcccggag gccgggaggg cgcgtgatca cctccgtccg
     1201 caacccgaag gtcgccgcgg ctctcaagct ccacaagcgc gcgttccgcg agcgcgaacg
     1261 cgcgttcctg gtcgagggcg tgcaggcact gggcgaggcg ctctcgatgc cccgcggcgg
     1321 gatctcggcc ctgttccaca cggagccctc gcatcccctg gtcgaacgcg cacgcgccgc
     1381 gggcgtcgcc gcgacacacg tgagcgacga cgtgatggct cgcctcgccg gcacggtcac
     1441 cccgcagggg ttcgtcgggg tcgcggggta cctcgacgtc ccgttcgacc acctgcccga
     1501 tgagccggcc ggggtggccc tgctgcacga ggtccgagat ccgggaaacg cagggacggt
     1561 gctccgatcc gccgacgccg tcggtgccga cgccgtggtc ttctcctcgt cctcggtgga
     1621 cgtctacaac gcgaagaccg ttcgcgcctc ggcgggctcg ctgttccacc tgccggtcgt
     1681 gcggggctcc gacaccgcgt cggcggtcgc ggcgctgcgg gcgcggggga tgcggatcct
     1741 cgcgatggac gccgacggcg agggcgacct ctacgaggag gacctgtccg atcccgtcgc
     1801 gttcgtgttc ggcaacgagg cgtggggtct gccgaaggag atctctgccc tcgccgacgc
     1861 gaccgtgcgg gtgccgatca cgggtcgagc cgagtccctg aacctcgccg cggccgcgac
     1921 cgtctgtctg ttcgaatggg agcgccagcg tcgcgagggc aggcgcgcgg tgctcgagac
     1981 gatcatcgcc gccgccgctc acgacatccg ctcgccgctc accgcgttga aggggatcgg
     2041 gcacgccctc gctacccggt gggagcagat gaggcccgag gaccgtgagc tgatgctgcg
     2101 ggcgatccag ttcgacaccg atcgcctgaa cgcgatcctg cggcagctcg tggacgccgc
     2161 ccgcgtcgcc gccggtgcgc tcgatctgtt cccgcaacgg acttccgtct cgcatgtggt
     2221 cgaggagatc gccacgtccc tggcccgcga ccccgatcac gtccgcgtcg agtgggcggc
     2281 cggcgacgac ctgaacgtct tcgtcgaccc cgaacgcctc aggctcgtcg tcgaggcgtt
     2341 cgtcgaatcg ctcgtctggt gggcgagcga gggtccggtg cgcgtcgagg gacgcgttcg
     2401 ggacggccga ctctcggtcg aggctcgccg cgccgagacc gagctcacgc aggagcaggc
     2461 ggagcgtctg ttccgccccc gcgcgccggg gaccggttcc ggaagcaaga tcgggctgtt
     2521 cgtggcgctc ggcgtggccg aggcccaggg gggcggcgtg tccgcccgcg tcgccgacgg
     2581 cacgctcatc ttcgcgctcg acctccccgt cccgtccgag ccggacgcct gacgaacgcg
     2641 cgcgagggcg cgcgtctcgg aaggccagaa agggcccttg ctagactcgc cgcaccatgg
     2701 acccgtccga ggccgcgcgc atcctcgagc aggaactcga gcgcggacgc gagctgatcg
     2761 acgcggcgcg gacggtcgag gagctcgacg aggcgaagac ggcggtgctg gggcgcaagg
     2821 ccccgatcgc gagcgtgcag agctcgctcg gggcgctgcc cgccgacgcc cgccgtgacc
     2881 tcggccggcg gaccaacgag gccttcgcgg agctgcggac cgaactcgat gcgcgccgcg
     2941 ccgagctcga ggagggcgcc gaggacgagc tgctccgggc cgacgcggtc gacgtcaccc
     3001 tgcccggccg gcgcccgcgt ccgggctcgc tgcacccgct cagcctcgtc caggaccgga
     3061 tcgtcgagat cttcacgcgg atggggtacc gggtggccgt gggccccgag atcgaggacg
     3121 agtggcacaa cttcgaggcg ctcaacatcc cccccgacca ccccgcgagg acgatgaagg
     3181 actccctgta cgtcgacgcg gacgggctcc tgctccgcac cgaaaccagc tcggttcaga
     3241 tccggacgat ggagtcgcag gaaccgccga tctcggtcat cgtgccgggg cgcacctacc
     3301 ggcgcgaggc cgtcgacgcg acgcacctgt cggtcttcca tcaggtcgag ggactcgtgg
     3361 tcgatgaggg gatctccttc gccgacctcc gcggcacgct ggaggccttc gcccgcgaga
     3421 tgttcggcga ggacgtgcga gtgcggctca cgcccgacta cttcccgttc gtcgagcccg
     3481 gctgccaggc ggcggtgtcg tgcttcgtct gcggcggcgt cggctgccgc gtgtgcggga
     3541 acgggtggct cgagctgctc ggcgccggca tggtccaccc gaaggtgctg gagaactccg
     3601 ggatcgactc cgagcggtac acggggttcg cgttcggcat ggggatcgag cgcgtcgcga
     3661 tggtcaggta cggcgtcaac gacatgcggc tgctcgtcga gggtgacgtt cgcttcctcg
     3721 aacggttcga gggcgcggcg tgagggtcgt cctgtcctgg ctccgcgaga tgtgtccgac
     3781 cgaacggtcc gccgaggatc tggccgagct gctgaccgcc aagggcgccg aggtcgaggc
     3841 gatcgactac ccgtgggagc gggtctccgg tgtcgtggtc gccagggtcc tcgaggtccg
     3901 cgaccacccg aacagcgaca ccctgtgtct cgcccgtgtg cagaccggtt cgggcgagct
     3961 cgaggtggtc gtcggcgttc ggaacatggc tcctggcgac ctcgtgccgc tggcgccgcc
     4021 cggcgcgagc gttctcacgc tccccgaacc cctggaggcc cgcgagatcc gcggcgtcgt
     4081 ctcccagggc atgctgtgtg cgcccgacga gctcggcata tcggcgtcac acgaggggat
     4141 cctcgtgctg ccgggcgacc tgcagccggg cacggacgtc gccgaggcgt tcgggctgac
     4201 cgacgcggtc ctcgacatcg aggtcacgcc caatcgaccc gacttcctgt ccgtgctcgg
     4261 gatcgcgcgg gaggtgtcgg cggcgacggg gacccctttg tcaccaccgg acaccgacgt
     4321 gaaggaggac gccgagcccg ccgagggggt cgcgaccctg gaggtcgcgg atctcgaacg
     4381 ctgcccgcgt tacctcgcca ggatcgttcg cgacgtgcgg cacgtgccct cgccgatcgc
     4441 cgcgcaggcg cggctgttcg cggccgggat gcgtccgatc tcggcggtgg tcgacgcgac
     4501 caactacgcg atgctcgaga tagggcagcc gctccacccg ttcgatctcg cgctcctgaa
     4561 ggggcccggg atcgtggttc gtcgcgcggt ggcgggggag acgctcgtga cgctcgacgg
     4621 cgttgagcgg gcgttcaccg aggacgatct gctgatctgc gacgcggagc gaccggtcgg
     4681 tgtggccggg gtgatgggtg gcgagctcgc cgaggtctcc gagcagacga gtgacctgct
     4741 gctcgaggcg gcgtggttcg aacgcggagg cgtccaacgc acccggcggc ggatcggcct
     4801 gtcgacggag gcgtcgattc ggttcgagcg tggcgtcgat cccgaggccg cgccgacggg
     4861 cgccgaccgg gcgtgtcggt tgatgtccga gtggtgcggc gcgcgcgtcc tccgcggcct
     4921 cgtcgaggtg ggcggcccgc cgccacggcg ccggatcgag cttcgggcgc cgcgggcctc
     4981 ggcgctgatc gggtacgcgg tgaagaacgt ggacgccgtg gaggtgttcg agcgactggg
     5041 gatcgggacc gagaccgtcg acgaggacac gatcctggtc gagatcccgg gctaccgcgt
     5101 cgacctcgag cgcgaggtcg acctgatcga ggaggtcgta cgtgtccagg ggtacgagcg
     5161 cgtcggctcc acgctcccgc cgatcgcgcg gccgggcggg ctgcccgccg catacggctt
     5221 ccggacccgc gtccgtgacg ccctgcgacg ggccgggctg cgcgaggttc ggcagatccc
     5281 gttcgtctcc gaggccgacc tccggatggt gggggacgac gacgcgatac gggtcaccaa
     5341 cccgctccag gcggacgacg gatggcttcg gacgcgtctg acccccggga tgctgaaggt
     5401 cgtccggcac aacgcctatc gccacgtccg gtcggtcgcc gtcttcgagg tcgacaccgt
     5461 ctttcggatg cgcgacgggc ggcccgacga acgcccgaag gccgcgttcg cgctgaacgg
     5521 gaacgcggat cccgggtggg cgcgcgacga ccgcccgttc gacgtgttcg acgcgaaggg
     5581 cgtcgtcgag gccgtgatgg ccgagctcgg gatctcgtgg accctcgggg acccggtcgg
     5641 ccgcccgttc caccccggca ggtccggggc gatcatggtc ggcgaggagc ggatcggcgt
     5701 cctgggcgag atccatccgc gcgtcgccga tcggctcggc ctcaccgggc gggtcgccgt
     5761 ggccgagctc gaggtcgagg ccctgatgcg cctcgcgcgc ccggtgtcgg gtgtggagga
     5821 cgtgccgcgc ttcccgccgg tccgccgcga cctggccttc accgtcgacg cgtcgatccc
     5881 ggccgggcgc gttcgctcgg cgctcgagga cgcggcaggc gagatcgtcg gctcggtgct
     5941 cctgttcgat gtgttcgagg gaccgccgct gcccgaggga accaagagcc tcgcctactc
     6001 ggtcgatttc cgcgtccccg accggacgtt gaccgatgag gaggtcgacg gcgcggtgga
     6061 cgcgatcgtc caacggctgt cgcgcgacgt cggcgcgcgg ctccggtcgg gctgaacccg
     6121 cggggctcga cccggatcgc gaccggcgtc ctctcgcggg cggggtctgc gagcagcggg
     6181 tcggacccgc ggtctagact cgaacgatgg agcgcgacct gctgtcgatc gacgacctct
     6241 cgccgaggga gctcggccag ctcctcgagc tctccgccaa ggtcaaggcc cagccggcgg
     6301 actacgccga tcgcctcgcc gggcgctcgg tcgcgatgat cttcgagaag ccctcgaccc
     6361 gtacccgggt ctccttcgag atcgcggtcg cctcgaccgg tggccacccg gtgccgctgt
     6421 cctccgcgga tctgcagctc ggacgtggcg agacgatcga ggataccggg cgggtcctgt
     6481 cgaggtacgt ccaggcgatc gtgctgcgga ccttcgagca ggaacggctg gaggtgctgg
     6541 cctccgcggc ctcgatcccg gtggtcaacg cactctccga tttcgagcac ccgtgccagt
     6601 gcctggccga cctgctgacg atcaaagaag tgaaggggga gctcgccgga acgatcgtca
     6661 cgtatctggg ggacggcaac aacgtgacgc actcgttgct attcggcggc gcgaaggcgg
     6721 ggatgcacgt gcgtgccggg acgccgcccg gtttcgagcc gattccgcag gtcgtgcagc
     6781 gcgcgtccga catcgcgtcg cagaccggcg ggagcgtgac catcacgcac gacccgatcg
     6841 aggcgtcgcg cggcgcggac gtcctgtaca cggacgtgtg ggcgagcatg gggcaggagg
     6901 ccgaggccga cgagcgcgcc ctcgttttcc cggcctacgc gctggacgcc gagaaggtgg
     6961 ccgtcgccgc cccggacgtc acggtgatgc actgcctgcc ggcccaccgg gggcaggaga
     7021 tcgccgccga cgtgatcgac gggccgcatt cggtcgtgtg ggaccaggcc gagaaccgga
     7081 tgcacgcgca gaaggccctc atgctgtggc tcctcggtct ggcctgagcc cgatccggga
     7141 ggacccgtcg tggcgcgtct gaacgatacc gtcgaggcgg cgttgatcga atacgcggac
     7201 cttctgtcga tcctgaccga agacccctac aagccgcgct cctacgagaa ggccgctcgg
     7261 gcggtgggtg gctacccgga cgacctggcg acgatggacc tgaaggagat cctggggatc
     7321 ccgagcgtgg gcaagtcgat cggcgagaag atcgacgagt tcgtccggac gggctcgttc
     7381 gaggcgctcg acgagctgcg cgcgcagatc ccgccggggg tgcgcgagat gaccgcgatc
     7441 ccgggactcg ggccgaagaa ggcgatcctg ctctaccgcg acctcgggat cgccgacgtc
     7501 gaacagctgg cccaggcggt ggaggaaggc cgcctggccg aggtcaaggg gttcggcgcc
     7561 aagaccgagg agaacatccg ccgaggcatc gagcgcatga ctcagtcgac gggtcgggtg
     7621 ctcgtgtccg tcgccatgga cctcgcggac tacttcatcg agcggctggg ttctcgtaag
     7681 gacgtgcgcc ggatcgagtt cgcgggctcg ctccggcgga tggccgagac gatcggcgac
     7741 ctcgacctcc tcgtcgcgag cgacgacccg gccggggtga tggaggcgtt cacgacgatc
     7801 gggatgaccg aacgcgtgct cgccaggggt gagacgaagt cgtcgatcct gacccgcaag
     7861 ggggtgcagg tcgacctccg cgtcgtcccg ctcgaggcgt ggggcgcggc gatgatctac
     7921 ttcaccggct cgaaggccca caacatccgg atccgcgaga tggccgtccg caagggtctc
     7981 aagctcaacg agtacggcct gtatcgggcg aagtcgggca agctcatcgt cgccgagacc
     8041 gaggaggagg tctacgagaa gctttcgctc tcgtggatcc cgccgacgct ccgcgaggat
     8101 cgcggcgagg tcgaggccgc gctggagggc accctccccg atgtgctcgt gcgcgggcag
     8161 ctgcggggcg acctgcacac gcacaccaac ctcacagacg gcctggcctc gctcgagcag
     8221 atgctggcgg cggcctccga gctgaagtac aagtactacg cggtgacgga ccacgcgccg
     8281 aacctgttca tgcagcggat gaccgacgag aagatgctcg cgcagcggga gcagctccgt
     8341 gcgctgcaga gcgcgtaccc gaagatgacc ctgctgcacg gcaccgagct caacatcgac
     8401 gctgagggca acgtggactg ggacggcgac ttcctcgagg ggttcgacgt ctgcgtcgcg
     8461 tcggtgcact cgctcttcaa ccagccgaag cacgagatga cccgaaggat cgtgcgggcg
     8521 atggagaacc ccaacgtgca cgtgatcggc cacccgaccg gccggatgat caacaagcgc
     8581 cagccggtcg aggtggacct cgaagaggtg ttcaaggccg cggcgcgcac ggggacggcc
     8641 ctcgaggtga acggctaccc ggaccggctc gatctgcggg acgagcacat cctgtgggcg
     8701 cggcgccacg gcgtgaagtt ctcggtcgac accgactcac acgccacgac gcatctcgag
     8761 cacatgcggt acggcgtggc gaccgcacag cgcgggtggc tcaccgagcg cgatgtgatc
     8821 aacgcgtggc cgctctcgaa gctcagggcg ttcctgcgga agggccggtg agacgcacgc
     8881 cgggcggcgc gacggggttc ggccgccggc taccgcgctc gttcttcgca cgaccgtcga
     8941 ccgtcgtcgc ccccgagctg ctcggccgcg tcctggtgag gatccggccc gacggcacgc
     9001 ggctcgccgg acggctcgtc gaggtcgagg cgtacgagca gggcgacccg gcgagccact
     9061 cgttccgcgg tcgccccacg cccaggaccg aggtgatgtt cggccccccg gggcggctgt
     9121 acgtgtattt cacctatggg cagcatttct gctcgaacgt ggtgaccggc cccgaggggt
     9181 acgggagcgc ggtcctgctc cgggccgccg aaccgctcga cgggttcgag acgatggcgg
     9241 cgaaccgagg gaccgacgcg gtccggctcc tgtgttcggg cccggcgcgg ctgactcggg
     9301 cgctcgagat cgatcgcgcg gacaacggga cggacgtggt gaaggatccc tcgttgttcc
     9361 tgctcgccgg ctcgccggtc ggcccgcgcg cgaccggccg gagcaccagg gtgggtgtca
     9421 acgtcggggt cgaacacgtg tggcgcttct tcgaacgggg gaacgcgttc gtgtcgccgg
     9481 gcaagccttc ggtccccgat gtcgacgggg cgcagcgaag ggctacgggt tcacgaagat
     9541 cgtgatggtc gacccccgct cccgctgcgc gccggcgtcc ggcgactgcg accacacgac
     9601 gcccggccgg tcgtcgcacg aggggtcgtc cgggtcgcac tccccagtgg acaccgacgc
     9661 gacgaacccg gcctgctgga tctccgcctc cgccgcgcca cgcggcatgc cgaccacgtt
     9721 cggcgccgga gcgaggtcgg gtgcggggga aggttcgggc gcgcccctgg ccaccgtgat
     9781 cgtgaccgtc ccggtcagga tcagccgcga gccgcccccc ggatcctgcg cgatcacggt
     9841 gcccggctgc cggtccgacc gcgcgagcac cgtcgcgacc atgaacccgg cgttgtggag
     9901 cgccgagatc gcttcctggc gctcgaggcc gatcaccgac ggcaccaccg gcaactggta
     9961 cgaggacggc tccgagcaca cctcgagcgt cggctcggcg ccggagggga accgaagcac
    10021 gtcgacgtcc ccgggcggcg tataggggtt cgcgagacac ccctgggtga tgtcgacacg
    10081 cagcgtcacg aaggcgacgt tcggcgacgt gccgaactcc ttcacgggga gcccccgggt
    10141 cgccgcgagc atgaacgcat gccagatcga ggccggccac gtccccccgt agacggtcga
    10201 gatccgcacg ttgccgcagc acatcgggat ctgcccctgc gggaacccca cccacaccgc
    10261 ggtgaccaat tgggggaccg cgccgacgaa ccacgcgtcg ctcgcgttct gcgcggtccc
    10321 ggtcttgccg aactgcggcc gcccgatgtt cgcgcccacg cccgtacccg agctgaccac
    10381 gcccttcagg atgtcgacgg cctcggccgc gatggcgggt tccaccgcct gcttggcccg
    10441 cgagggagcc tggtagatca cgtccccgtc ggacgaggtc accgagatca cgggtgtcgg
    10501 ctgcacgtgc cggccgaggt tcgcgagcgt gccgaacgcg gaggccatct cgagcgtgct
    10561 cacttcgttc acgcccagga cggcggcggg cacggccgcc agcgggccgt tcggctcggt
    10621 ggtgcggggg cagcaccgga tgcccatgcg caccgcggcc tcgacgagcg cggcggcgcc
    10681 cgcgtaggga tcgccgtccc cgatcaccga gagcaggttc gcgtacgcca cgttgaccga
    10741 gttcacggtg gcgctctcca gcgagatcgt cccgtagccg ctgccctcgg cgttgtgagg
    10801 gtcccagtac gagccgtcct gcaacaggat gtgcgcggtg gagccgttga gcggctgcgt
    10861 cctcgggacg ccgtgttcga gggccgcgac gagcgcgaac ggcttgaacg ccgacccggc
    10921 ctggcgacct gtgacgccgc cggtcgcgag gttgatccgg gcgaagtcgt cgtcctcgtt
    10981 ccagtagtcg cgaccgccga ccatcgcccg cacgtacccg gtcctcggat cgatcgccgt
    11041 cagggccccg tagggatcgg ccggttcggt gagcaccgac ccgatcgccc gctcggcggc
    11101 gcgttgcagg cgcagatcga ccgtggtgac gatcctcagg ccgccctcga acaggaggtc
    11161 gtagcgatcc tggggcgtct cgccgaaccg ggggttcgag aggaaccact ccttcacgta
    11221 gtcgacgaac cagggcgccg ggtagtgcat cgcgtcctcc ggggcgaggt cgagggcgat
    11281 cggcgagcct ctcgccttct cgaactccgc ctcgtcgatc atgcccaggc cgcgcatctg
    11341 cgtgagcacg tggttgcggc gacgctcgga ccgtcccgga tgacggacgg ggtcgtagtc
    11401 gaccggcgct gcgatcaggc cggcgagcgt cgccgcctgc gacagggtga gctcgagcgg
    11461 ctcgacgccg aagtaggtct cggacgcggc caggatcccg tatgcgccgt tgccgaagta
    11521 cacggtgttg aggtacctgg tcaggatccg gtccttgctc agccgctgct cgagctgcca
    11581 cgcgaggtag gcctcgcgga tcttgcgccc gaccgtctgt tcgtcaccga cgtagagctt
    11641 cttcacgagc tgctgggtga tcgtcgagcc gccctcgacg accctgccgg tggtggcgtc
    11701 gacgtaggcg gcccgcagca gcgcccgtac gtccagcccc gagtgatcgt agaagcgttg
    11761 atcctcgctc gcgatcaccg cgtcgcgcat gacgtcgggg atcttccgcg agcggacgac
    11821 cacccggttc tcgccggcgt gcagcgtcgt gatcagcgct ccgtccgcgg agtagatgaa
    11881 ggaggtctcc gggagggcgt ccgcgctctt gcgcgcctcg tgcagggagg ggaggtcgca
    11941 cgcggatgct gcgagggcga acacgaacac gccgaccacg agggtccgcg agcgaggggc
    12001 catggtccca ccatcttgcc acgggtggaa gcacgccact gctagccttc gcgccatggg
    12061 accgaaagac ctcccgaccc tgctcgcagg ttccgccgag gtcgtgccgg cggaggatct
    12121 cgagcacaaa ctgagcctgg ggcggccgct gcgagtcaag ctcggactgg atccgaccgc
    12181 gccggtcgtc acgctcggat gggccgtggt gctccacaag ctgcggcagt tccaggacgc
    12241 ggggcacacc gccgtcctga tcgtgggtga cttcacggcg caggtcggcg acccctccgg
    12301 aaagaccgag acgcggccgc gcctgtccga ggaggaggtg cgtggccacg ccgagcggct
    12361 gctcgatcag ttctggaaga tcctcgatcc cgagcgcacc gaggtgaggt acaacgccga
    12421 gtggctcggg ccgatgggca tggaggcgat cctgcgactc acggcctcga ccaccgtcgc
    12481 gcggatgctc gagcgcgacg acttcgcgaa ccgcttcgcc gccagggagc cgatctcgat
    12541 ctcggagctc ctctatccgc tcatgcaggc gtatgactct gtcgcggttg cggccgatgt
    12601 cgagctcggt gggaccgatc agctctacaa cctgctgatg ggccgcgagg tcatggaggc
//
LOCUS       DATBRK010000127         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2328987, whole genome shotgun sequence.
ACCESSION   DATBRK010000127 DATBRK010000000
VERSION     DATBRK010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328987"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VIH70_03750"
     CDS             <1..315
                     /locus_tag="VIH70_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8115508.1"
                     /translation="YEVRTAGSGDRALELAQERCPDLILADVMMPNMDGFELTRRLRM
                     DERTAAAKVILLTARGLSADRLEGFAVGADDYVIKPFDTPELLARIGDVLARPGTTTQ
                     PA"
     gene            350..>1875
                     /gene="hutU"
                     /locus_tag="VIH70_03755"
     CDS             350..>1875
                     /gene="hutU"
                     /locus_tag="VIH70_03755"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY8115509.1"
                     /translation="MSAREIRAPRGTELSCRSWPQEAAMRMLMNNLDPDVAERPEDLV
                     VYGGTGKAARSWEAFDAIVRTLRTLRDDETMLVQSGKPVGVFRTHEMAPRVLISNSML
                     VPKWATWDTFRELEHAGLTMYGQMTAGSWIYIGTQGILQGTYETFAEAATQRFGGSLA
                     GTVTLTGGLGGMGGAQPLAVTMNGGVALCVEIDPARIERRIKTRYLDRESDDLDEVLR
                     WVDEARSASEAVSIGVVGNCAEVLPQLLERGFAPDLVTDQTSAHDPLGGYVPAGLSLE
                     EAARMREDDPDGYERRSFDSMARHVEAMDGFLAAGAVVFDYGNNLRAGAENGGLPHER
                     AYAYPGFVPAFVRPMFCEGKGPFRWAALSGDPEDISKTDRAVAELFPENERLHRWLEM
                     AEERVAFQGLPARICWLGYGERARAGLRFNEMVASGELSAPIVIGRDHLDSGSVASPY
                     RETEAMRDGSDAIADWPILNALVNTAAGAHWVSVHHGGGVGIGYSIHAGMVVVADGSE
                     "
BASE COUNT          282 a          618 c          719 g          256 t
ORIGIN      
        1 tacgaggttc gcacggcggg gagcggcgat cgcgcgctcg agctcgccca ggagcgatgc
       61 ccggacctga tcctggccga cgtgatgatg ccgaacatgg acggcttcga gctgacgcgc
      121 cggctgcgga tggacgaacg caccgcggcc gccaaggtga tcctgctcac ggccaggggg
      181 ctgtcggccg atcggctgga ggggttcgcg gtgggcgccg acgactacgt gatcaagccg
      241 ttcgacacgc ccgagctcct cgcgcgcatc ggcgacgtcc tggcgcgacc cgggaccacc
      301 acgcaacctg cctgagtcgc cgcgaacatc ccgtcctatc atcgcgagcg tgagcgcacg
      361 cgagatccga gcaccccgcg gcaccgagct ctcgtgccgc tcgtggccgc aggaggccgc
      421 gatgcggatg ctgatgaaca acctcgaccc cgacgtcgcc gagcgccccg aggatctcgt
      481 cgtctacggc gggacgggga aggccgcccg gtcgtgggag gcattcgacg cgatcgtgcg
      541 gacgctcaga acgcttcgcg acgacgagac gatgctcgtg caatccggca agccggtcgg
      601 cgttttccgg acgcacgaga tggcgccgcg cgtgctgatc tcgaactcga tgctcgtgcc
      661 gaagtgggcg acgtgggaca cgttccgaga gctcgagcac gccgggctca cgatgtacgg
      721 ccagatgacg gccggctcgt ggatctacat cgggacgcaa ggcatcctgc aggggacgta
      781 tgagacattc gccgaggccg cgacgcagcg gttcggcggg tcgctcgcgg ggacggtgac
      841 gctcaccggc gggctcggcg ggatgggcgg cgcgcagccg ctggccgtga cgatgaacgg
      901 gggcgtcgcg ctctgcgtcg agatcgaccc ggcgcgcatc gagcgccgga tcaagacgcg
      961 ctacctcgac cgcgagagcg acgatctcga cgaggtgctg cggtgggtgg acgaggcgcg
     1021 ttcggccagc gaggccgtct cgatcggggt cgtcggcaac tgcgccgagg tcctgcccca
     1081 gctgctcgag cgcggattcg cgccggacct ggtgaccgac cagacgagcg cgcacgaccc
     1141 gctgggcggg tacgtgccgg cggggctgtc gctcgaggag gccgcgcgga tgcgcgagga
     1201 cgatccggat gggtacgagc ggcgttcctt cgactcgatg gcccggcacg tcgaggcgat
     1261 ggacggcttc ctcgccgccg gcgcggtggt gttcgactac gggaacaacc tgcgtgccgg
     1321 cgccgagaac ggcgggctgc cgcacgagcg cgcctacgcg tacccggggt tcgtgcctgc
     1381 gttcgtccgg ccgatgttct gcgagggcaa gggcccgttc cgctgggccg cgctctcggg
     1441 cgaccccgag gacatctcga agaccgaccg cgcggtcgcc gagctcttcc cggagaacga
     1501 gcggctccac cgctggctcg agatggcgga ggagcgggtc gcgttccagg gcctgccggc
     1561 gcggatctgc tggctcggct acggcgagcg ggcgagggcc ggcctgcggt tcaacgagat
     1621 ggtcgcgagc ggcgagctct cggcgccgat cgtgatcggc cgcgaccacc tcgacagcgg
     1681 gagcgtcgcg agcccgtatc gcgagaccga ggcgatgcgg gacggttcgg acgcgatcgc
     1741 ggactggccg atcctgaacg cgctcgtgaa caccgcggcc ggggcgcact gggtgagcgt
     1801 gcaccacggc ggcggcgtcg ggatcggcta ctcgatccac gcggggatgg tcgtcgtggc
     1861 cgacgggagc gagaa
//
LOCUS       DATBRK010000128         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1028603, whole genome shotgun sequence.
ACCESSION   DATBRK010000128 DATBRK010000000
VERSION     DATBRK010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028603"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VIH70_03760"
     CDS             <1..370
                     /locus_tag="VIH70_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8115510.1"
                     /translation="GDLHGQVVVVNFWASWCVPCREEHDALAAAWGRYRDRGVVVLGV
                     SFEDTREAALEFRDELKGDWPLADDPGSRAAIAYGVFGVPETFVIAPDGTIAAKTTGA
                     LTYEWLTDEIEGALRAGNAE"
     gene            367..843
                     /locus_tag="VIH70_03765"
     CDS             367..843
                     /locus_tag="VIH70_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY8115511.1"
                     /translation="MSGRTRSFGVVAAAIAVVALAALIASGGSGGARTLDERVHEIGA
                     GLRCPVCLNLSVADSPSKLAGEMRTEIETQLRAGRSPEQIRAFFIDRYGEWILLSPPP
                     RGWNLLPWAVPIVGVVAGVALWAAFVRRRAPGTAGQVTETEHRRIDHELADLEEPG"
     gene            874..1767
                     /locus_tag="VIH70_03770"
     CDS             874..1767
                     /locus_tag="VIH70_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8115512.1"
                     /translation="MNPIAISFAVAGLALVAGAGVIAGFLREAPAPVEPLADPLDDRR
                     EALLRSLADLEEAHASGALDEAGYLRLREQTEGRMARVLRALDRRDAGPPRSTAMVAL
                     EDAEAGGGRTSAAEPRRVPPWAVAVLIGGTVLAVVIASLARDAEPQLRASAPQTSNDP
                     LGFFERRVEEHPDDLAARLDLAHRYLDAGMVERALSQYAVALELDPDDAEALAHVGII
                     LYQNGRPEEALRSVDRALATDPRYPEGLFIRGVILLRGLDRPDDAISAFETYLDAAPF
                     GVERQTAKDLIAEARAAADAT"
     gene            complement(1791..1934)
                     /locus_tag="VIH70_03775"
     CDS             complement(1791..1934)
                     /locus_tag="VIH70_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115513.1"
                     /translation="MAPNAGFVIAGYLVTALALGGYTLRLFARARTARRRSQAIADRR
                     RDG"
     gene            complement(1934..2662)
                     /gene="ccsA"
                     /locus_tag="VIH70_03780"
     CDS             complement(1934..2662)
                     /gene="ccsA"
                     /locus_tag="VIH70_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY8115514.1"
                     /translation="MSATAERALGKATSRSGEHLLGIATLVSLGISAVLSLVVAPPDA
                     VQGEVQRIMYIHVPAAWLAYLSFFVVFISSIAYLKTNKTRWDRVAAASAEIGVLFTSL
                     AIVLGMLWGKPVWGTWWTWDPRLTTTAVLLLIYVAYLAVRRITDNPSRRARWAAVIGI
                     VGFIDVPIVHLSVVWWRSLHQGPTVFRLGGPEIEGTMLLALLVGVAAFTITYAYLMTL
                     RLRVGRLEERAAREALAPLVGAER"
     gene            complement(2662..>2875)
                     /locus_tag="VIH70_03785"
     CDS             complement(2662..>2875)
                     /locus_tag="VIH70_03785"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY8115515.1"
                     /translation="VAESARTREAIFPMLVLPLVAPILIAGVRATDLAAAGRSGEVLS
                     WLGLLAAFDVVFLSVGVLVFGYLLED"
BASE COUNT          426 a         1024 c         1051 g          374 t
ORIGIN      
        1 cggtgacctg cacgggcagg tggtcgtcgt caacttctgg gcgtcgtggt gcgtgccgtg
       61 ccgggaggag cacgacgcgc tcgccgccgc ctggggccgg tatcgagacc gaggcgtcgt
      121 cgtcctcggc gtgtcgttcg aagacacgcg cgaggcggcg ctcgagttcc gcgacgagct
      181 gaagggcgac tggccgctcg ccgacgaccc gggctcgcgt gcggcgatcg cctacggagt
      241 cttcggggtc cccgagacct tcgtcatcgc gccggacgga acgatcgccg cgaagacgac
      301 cggcgcgctg acctacgagt ggctcaccga cgagatcgag ggagcgctcc gagcggggaa
      361 cgccgagtga gcggccgaac gcgttcgttc ggggtggtcg cggcggcgat cgcggtggtc
      421 gcgctggcgg cgctgatcgc ctccggtggt tccggcgggg ctcggacgct cgacgagcgc
      481 gtgcacgaga tcggtgcggg gttgcgctgt ccggtgtgcc tgaacctctc agtcgccgac
      541 tccccgtcga agctcgcggg cgagatgcgc accgagatcg agacccagct gcgcgccggc
      601 cgcagccccg aacagatccg cgcgttcttc atcgatcgct acggcgagtg gatcctgctc
      661 tcgccgcccc ctcgcggttg gaacctcttg ccgtgggccg tgccgatcgt cggcgtggtg
      721 gcgggcgtcg ccctgtgggc cgccttcgtt cgccggagag cgcccgggac cgccggccag
      781 gtgaccgaga ccgagcatag gcggatcgac cacgagctcg cggatctcga ggagcccgga
      841 tgaaccgggg acgtcggtga tcgggaggcg cggatgaacc cgatcgcgat ctcgttcgcc
      901 gtcgcggggc tggcgctcgt cgccggcgcc ggcgtcatcg ccggcttcct ccgcgaggcg
      961 ccggcacccg tcgagccgct cgccgacccg ctcgacgacc gccgggaggc gctcctgcga
     1021 tcgctcgccg acctcgagga ggcgcacgcg tcgggcgcgc tcgacgaggc cggctacctc
     1081 cggctgcgcg aacagaccga gggccggatg gcgcgtgtgc tgcgcgcact cgaccgtcgc
     1141 gacgccgggc ctccgcggag caccgccatg gtcgcgctcg aggacgctga agcgggaggc
     1201 ggtcgaacga gcgccgccga acctcgacgc gtcccgccgt gggccgtcgc ggtgttgatc
     1261 ggggggaccg tcctcgcggt cgtgatcgcg tcgttggccc gcgatgccga accccagctc
     1321 cgggcgtccg cgccgcagac ctcgaacgac ccgctcgggt tcttcgagcg acgcgtcgag
     1381 gagcacccgg acgacctggc ggctcggttg gacctcgcgc atcgttacct ggacgcgggc
     1441 atggtcgagc gcgccctgtc ccagtacgcg gtggcgctcg agctcgaccc cgacgacgcg
     1501 gaggcgctcg cgcacgtcgg gatcatcctg taccagaacg ggcgaccgga ggaggcgctc
     1561 cggtcggtcg accgggcgct tgcgaccgac ccgcggtacc ccgaggggct gttcatccgg
     1621 ggggtcatcc tcctgcgagg gctcgatcgt cccgacgacg cgatctcggc gttcgagacc
     1681 tacctggacg cagcgccgtt cggcgtcgaa cgtcagacgg ccaaggacct catcgcggag
     1741 gcccgagccg cggccgacgc tacctgatcc ggctacggcg ggggatgcgg tcagccgtcg
     1801 cgacggcggt cggcgatcgc ctgggaccgg cgcctggcgg tccttgccct ggcgaacagc
     1861 cggagcgtgt acccgccgag cgcgagcgcg gtgaccaggt acccggcgat cacgaagccc
     1921 gcgttcggcg ccatcagcgc tccgccccga cgaggggtgc cagcgcctcg cgcgcggcgc
     1981 gctcctcgag ccgccccacc cggaggcgga gggtcatcag gtacgcgtac gtgatcgtga
     2041 acgcggcgac cccgacgagg agcgcgagca gcatcgtccc ctcgatctcg ggcccgccga
     2101 gccggaacac cgtcggcccc tggtgcagcg aacgccacca gacgaccgac aggtgcacga
     2161 tcggcacgtc gatgaacccg acgatcccga tcaccgccgc ccagcgagcc ctccgagacg
     2221 ggttgtccgt gatccggcgg accgcgagat aggcgacgta gatcagcagg agcaccgcgg
     2281 tggtggtcag gcgcgggtcc cacgtccacc aggtccccca gacgggcttg ccccacagca
     2341 tccccaggac gatcgccagc gacgtgaaca ggacgccgat ctcggcggac gcggcggcga
     2401 cgcggtccca tctcgtcttg ttggtcttca ggtacgcgat gctcgagatg aagactacga
     2461 agaacgagag gtacgcgagc caggccgccg ggacgtggat gtacatgatc cgctgcacct
     2521 cgccctggac cgcgtcgggg ggcgccacca cgagggagag cacggccgag atcccgagcg
     2581 agacgagcgt cgcgatcccg agcaggtgct ccccgcttcg agacgtggcc ttgccgagcg
     2641 cgcgctccgc cgtcgccgac atcagtcctc caacaggtag ccgaacacca ggacgccgac
     2701 cgacaggaac acgacgtcga aggccgcgag cagtcccagc cacgagagca cctcgcccga
     2761 gcgacccgcc gcggcgagat cggtcgcgcg aacccccgcg atcaggatcg gcgcgacgag
     2821 cgggagcacg agcatcggga agatggcctc gcgcgtgcgc gcggactcgg ccacg
//
LOCUS       DATBRK010000129         5995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1028679, whole genome shotgun sequence.
ACCESSION   DATBRK010000129 DATBRK010000000
VERSION     DATBRK010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028679"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VIH70_03790"
     CDS             complement(<1..392)
                     /locus_tag="VIH70_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115516.1"
                     /translation="MGETADRPAFYALSPGGWRDYWSLLHPPYTAWHLSYVVIAACVA
                     PVVDVGWLVETLVAFFLAMGLAAHALDELNGRPLRTRIPHGVLWAISVVGLGGAIGLG
                     IHGTFVISPWLWAFIGAGGFLVVAYNLEL"
     gene            complement(385..1143)
                     /locus_tag="VIH70_03795"
     CDS             complement(385..1143)
                     /locus_tag="VIH70_03795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8115517.1"
                     /translation="MDPERTRQARRLFAGIAPEYDRMGSVLSVGQDPRWRRFLVSKVN
                     AIPGSWVLDVATGTGLVARELANKNVRVVGLDQSRAMVARGVEAVRRRGLEGRVRFVL
                     GQAQALPFADGSIDAVTFTYLLRYVDDPAATVAELVRVLRPGGVMASLEFHVPPEPWA
                     RAGWLAYTRSAMPLVGWTVSNEWYRTGRFLARSITEFVDRYPLPVQVRWWQEAGMRRV
                     RTKLLSSGAAVVTWAVKSTPVLGQESEDLAHPDG"
     gene            complement(1191..1970)
                     /locus_tag="VIH70_03800"
     CDS             complement(1191..1970)
                     /locus_tag="VIH70_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HEY8115518.1"
                     /translation="MDEIEGVLLDIDGVLAVSWEPLPGAIDTIGWLRSNGVPFRLITN
                     TTTKTRAELARTLRDAGFDVRDDELITAVAATARYLRANHPNARCFLLSDGDAAADLE
                     GVELADLDVADVIVLGGASEAFTYTTMNRVFGRLMDGATLVGMHRNLYWRTSDGLELD
                     AGAYIAGLEEATGREAVICGKPSGPYFDAALAMLGVPREGAIMVGDDIVSDVRGAQVH
                     GLRGVLVRTGKYLPDDLAKVPDPPDHVLETIADLPGLLGSS"
     gene            complement(1980..2864)
                     /locus_tag="VIH70_03805"
     CDS             complement(1980..2864)
                     /locus_tag="VIH70_03805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEY8115519.1"
                     /translation="MADLGYFGPDSVSWRVHREVTVLFGGARAVLMHAAHPLVIAGAR
                     ETGFYERNPWKRLQRTLVLTYTITFGSKAEADAAAERINDVHARVKGVDTETGLPYDG
                     LDPDLLLYVHACLIESALVFERLTVGGLDAAGRQRFHEEQMLAAEMVGIPRAAIPPTV
                     PELRAYLQGVYDSGIPRVGEAARRVAALFHDPPREAEWRPVLIGVSKLAFGTLPPELR
                     RLYGIEVGPGRRMAMRAAFAATRALRPLLPPRFRFIAPYQEWRTRTSGGVRDARRSVG
                     VRVDTARSLGDEASNARR"
     gene            complement(2885..4540)
                     /locus_tag="VIH70_03810"
     CDS             complement(2885..4540)
                     /locus_tag="VIH70_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEY8115520.1"
                     /translation="MVPFPMLPTDSAAERRLYEGFLEQLDDAFVIYHSVDWVVGGRAG
                     PDEGEADFVITHPELGLLALEVKGGRVAYDPATNRWTQTGHSGTHGLDEDPFHQSEGA
                     MRSLVRILEGQPGWDRWKPSYGFGVAFPDGRYETAAHPGAPVEIVIDRGDLARLAERV
                     PEVMRTWARGGRRFGAEGMEAVSKALGFSVEVRTPLGLRFDEEDKKIVELTSDQAWVL
                     AFVLHRRRAAVTGPAGSGKTVLAVSVAKQLATAGHRTLLTCFNRRLGEHLRASVGGFG
                     QIDTATFPELCVRLAKEAGVDLPPEEAGPGSPYLERRLPEALAEAATRLGPRYEAIVV
                     DEGQEFRAWWWPALLSLHTDPDAGLLYVFADDNQNLYGGALPVGEEDTIGPISLNLRN
                     TKQIGEFVSVFYKGEQEPIARGPEGEPVQILGYDGEDDLEHLLAVVLRNLVEEEHVPL
                     EDIVVLTPSGTGTSRLRRTGMVDGYRLSETVEPGSVLATSVHAFKGLERPVVILAELG
                     DEHLEDLRQHLYVGGSRARNHLVVLAAEPVARELRAITGVTHP"
     gene            4614..5282
                     /locus_tag="VIH70_03815"
     CDS             4614..5282
                     /locus_tag="VIH70_03815"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HEY8115521.1"
                     /translation="MKLLLAVVVLSVCLGYIFGGRLHHLEALRPRWWGLVLLGLGIQF
                     VHLPDGVAGIDLVIRTSALALSYSLLLAFALVNVRMPGMLLVVVGLACNMTVILVNGG
                     MPASAKALIDSGQQDVLASLRHEAEEKHHLLTDDDQLTFLADVIAVPQPIGQAVSVGD
                     IIVYAGLTWLIVAAMRGWTPSARPAGSGQRRGKHRRGATRGPEPLPDFGFLPPGARTS
                     GTGP"
     gene            complement(5323..5967)
                     /locus_tag="VIH70_03820"
                     /pseudo
     CDS             complement(5323..5967)
                     /locus_tag="VIH70_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
BASE COUNT          888 a         2175 c         2021 g          911 t
ORIGIN      
        1 agctcgaggt tgtacgcgac gaccaggaac cctccggcgc cgatgaacgc ccacagccac
       61 ggcgagatca cgaacgtccc gtggatcccc agcccgatcg cgccgccgag cccgacgacc
      121 gagatcgccc acagcacgcc gtgggggatg cgcgttcgga gcggccgccc gttgagctcg
      181 tcgagcgcgt gggcggccag gcccatcgcg aggaagaacg cgaccagcgt ctcgaccaac
      241 caccccacgt ccaccaccgg cgcgacgcac gccgcgatga ccacgtagga caggtgccat
      301 gcggtgtacg gcgggtggag cagcgaccag tagtcgcgcc agccgcctgg gctcaacgcg
      361 tagaacgccg ggcggtcggc ggtctcaccc atcggggtgg gcgagatcct cggactcctg
      421 ccccaggacg ggtgtcgact tcaccgccca cgtcacgacc gcggcgccgc tgctcagcag
      481 cttcgtccgc acgcgacgca tccccgcctc ctgccaccat cggacctgga cggggagggg
      541 gtaccgatcg acgaactccg tgatgctccg cgcgaggaac cgaccggtcc ggtaccactc
      601 gttggacacg gtccatccga ccagcggcat cgcggacctc gtgtacgcga gccatccagc
      661 ccgggcccag ggttcgggcg gcacgtggaa ctcgagcgag gccatgacgc cgccgggcct
      721 taggacgcgg acgagctcgg cgacggtcgc ggcggggtcg tcgacgtaac gcagcaggta
      781 ggtgaacgtg accgcgtcga tcgagccgtc ggcgaagggc aacgcctggg cctgcccgag
      841 cacgaaccgg acccgcccct cgagcccgcg tcggcgcacg gcctcgacgc ctcgggccac
      901 catcgcccgg ctctggtcca ggccgaccac gcgcacgttc ttgttcgcca gctcgcgcgc
      961 gaccagcccg gtcccggtag cgacgtcgag cacccacgag cccgggatcg cgttcacctt
     1021 cgacacgagg aagcggcgcc acctcgggtc ctgccccacg gacaggaccg aacccatccg
     1081 gtcgtactcg ggcgcgatcc ccgcgaacag ccggcgagcc tggcgcgtgc gttcggggtc
     1141 caccgcccga gcctaccggc acagccggtc gggaggctcc cggctgaagg ctaggacgag
     1201 ccaagcagcc ccgggaggtc ggcgatcgtc tcgagcacgt ggtccggcgg gtccggcacc
     1261 ttcgcgaggt cgtccggcag gtacttcccg gtccgcacga gcacccctcg gagaccgtgg
     1321 acctgcgccc cacggacgtc gctgacgatg tcgtcgccga ccatgatcgc gccttccctg
     1381 gggacgccga gcatcgcgag cgcggcgtcg aagtagggcc ccgacggctt cccgcagatc
     1441 accgcctcgc gtccggtcgc ctcctcgagt cccgcgatgt aggcgcccgc gtcgagctcg
     1501 aggccgtcgc tcgtgcgcca gtagaggttc cgatgcatac cgacgagggt cgcgccgtcc
     1561 atcagcctgc cgaagacgcg gttcatcgtc gtgtacgtga aggcctcgga ggccccgccc
     1621 agcacgatga cgtcggcaac gtccaggtcg gccagctcga cgccctcgag gtccgcggcc
     1681 gcgtcgccgt cggacaggag gaagcagcgc gcgttcggat ggttggcccg gaggtaccgt
     1741 gcggtggcgg ccaccgcggt gatgagctcg tcgtcacgca cgtcgaaccc cgcatcgcgc
     1801 agggtcctcg cgagctctgc ccgcgtcttg gtcgtcgtgt tcgtgatcaa ccggaacggg
     1861 accccgttcg aacgcaacca tccgatggtg tcgatcgcgc cgggcagcgg ttcccacgac
     1921 acggcgagga cgccgtcgat gtcgagcagg acgccctcga tctcgtccat gcgcgaagtc
     1981 tagcgacgtg cgttcgacgc ctcgtcgccg aggcttcgcg ccgtgtccac gcggacaccg
     2041 accgaacgcc gggcgtcccg cacccctccc gacgtccgcg ttcgccactc ctggtacggc
     2101 gcgatgaagc ggaaccgtgg cggcagcaac ggccgtaggg cccgggtcgc ggcgaacgcc
     2161 gcgcgcatcg ccatccgccg gccggggccc acctcgatcc cgtacagtcg gcggagctcc
     2221 ggcggcagcg tgccgaacgc gagcttcgac acaccgatca gcaccggtcg ccattcggcc
     2281 tcgcgcggcg ggtcgtggaa cagcgcggcc acccgccgcg cggcctcgcc gacccgaggg
     2341 atgcccgagt cgtacacgcc ctgcaggtag gcgcggagct cggggacggt tggcgggatc
     2401 gccgcccgcg ggatccccac catctcggcg gcgagcatct gttcctcgtg gaagcgctga
     2461 cggccggccg cgtcgagccc gcccaccgtg aggcgctcga acacgagcgc gctctcgatc
     2521 aggcaggcgt gcacgtacag gaggaggtcc gggtcgagcc cgtcgtacgg caggccggtc
     2581 tcggtgtcga cgcccttcac gcgggcgtgg acgtcgttga tgcgttcggc ggcggcgtcg
     2641 gcctccgcct tcgaaccgaa cgtgatcgtg tacgtgagga ccagggtccg ctggagccgc
     2701 ttccacgggt tccgctcgta gaagcccgtc tcgcgcgccc cggcgatcac gagtgggtgc
     2761 gccgcatgca tgagcaccgc ccgtgccccg ccgaacagca cggtcacctc gcgatgcacg
     2821 cgccacgaga cgctgtcggg cccgaagtac cccaggtcgg ccacccagcc agcctagcga
     2881 cgtctcacgg atgggtgacg cccgtgatcg ctcgtagctc gcgcgcgacg ggttcggccg
     2941 cgaggaccac gaggtggttg cgcgccctcg acccaccgac gtacaggtgc tgccggaggt
     3001 cctcgaggtg ctcgtccccc agctcggcga ggatcaccac gggacgttcg aggcccttga
     3061 acgcgtgaac gctggtcgcg agcaccgaac cgggctcgac ggtctctgac agtcggtagc
     3121 cgtcgaccat ccccgtcctc cgcaggcggc tcgtgccggt tcccgatggc gtcagcacga
     3181 cgatgtcctc gagcggaacg tgctcctcct cgaccaggtt ccgtaggacg accgcgagca
     3241 ggtgctcgag atcgtcctca ccgtcgtacc ccaggatctg gacgggctcg ccctctggcc
     3301 ctcgggcgat cggctcctgc tcgcccttgt agaagaccga gacgaactcg ccgatctgct
     3361 tcgtgttccg gaggttgagc gagatcggtc cgatcgtgtc ctcctcgccc acggggagcg
     3421 ctcccccgta gaggttctgg ttgtcgtcgg cgaacacgta caggagcccg gcgtcgggat
     3481 cggtgtgcag cgagagcagc gctggccacc accacgcacg gaactcctgc ccctcgtcga
     3541 ccacgatcgc ctcgtaccgg ggcccgagcc gggtcgcggc ctcggcgagc gcctctggga
     3601 gccgccgctc gaggtacggg gaaccgggcc ctgcttcctc cggaggcagg tcgactcccg
     3661 cctccttcgc cagccggacg cacagctccg ggaacgtcgc ggtgtcgatc tggccgaacc
     3721 ctccgacgga cgcgcggagg tgctctccga gtctccggtt gaagcacgtg agcagtgtcc
     3781 ggtgcccggc ggtcgcgagc tgttttgcta ccgaaactgc cagcacggtc ttgccgctcc
     3841 ccgccggtcc ggtcacggcc gcgcgtcgcc ggtggagcac gaacgcgagc acccacgcct
     3901 ggtccgaggt gagctcgacg atcttcttgt cctcctcgtc gaaccgaagc ccgagcggcg
     3961 tgcggacctc gacgctgaac ccgagggcct tcgagacggc ctccatgccc tccgcgccga
     4021 agcgtcgccc cccacgcgcc cacgtcctca tcacctcggg gacgcgctcg gcgagccgtg
     4081 cgaggtcgcc ccggtcgatc acgatctcga cgggcgcgcc cgggtgcgcc gcggtctcgt
     4141 acctcccgtc ggggaacgcc accccgaacc cgtagctggg cttccaccga tcccatccgg
     4201 gttggccctc gaggatccgc accagcgaac gcatcgcacc ctccgattgg tggaacgggt
     4261 cctcgtcgag accgtgtgtc ccggagtggc ctgtctgggt ccagcgattc gtggccgggt
     4321 catacgccac ccgaccgccc ttgacctcca gggcgagcag ccccagttcg gggtgggtga
     4381 tcacgaagtc ggcctcgccc tcgtcgggac ctgctcgccc accgacgacc cagtccaccg
     4441 agtggtagat cacgaacgcg tcgtcgagct gttcgaggaa tccctcgtag agacggcgtt
     4501 cggcggcgct atcggtgggg agcatcggga acggcaccat cctggccacg ccgggatgct
     4561 agccccgtcc ctggacacgg gccgcggcgc atggcaccct ccgttcccta ccgatgaagc
     4621 tcctgctcgc cgtcgtcgtg ctgtccgtct gcctcggcta catcttcggc gggcggctgc
     4681 accacctcga ggcgcttcga ccccggtggt gggggctcgt cctcctgggg ctcgggatcc
     4741 agttcgtcca cctccccgac ggcgtcgcag gcatcgacct cgtgatccgc acgtcggcgc
     4801 tggcgctctc ctactcgctc ctgctggcgt tcgccttggt gaacgttcgg atgcccggca
     4861 tgctcctggt ggtggtcggg ctggcctgca acatgaccgt gatcctggtc aacggaggca
     4921 tgccggcgag cgcgaaagcc ctgatcgact cgggccagca ggacgtgctc gcctccctgc
     4981 ggcacgaggc cgaagaaaag caccacctgc tgaccgatga tgaccagctc accttcctcg
     5041 ccgacgtcat cgccgtcccg cagccgatcg gtcaggccgt gagcgtgggc gacatcatcg
     5101 tgtacgccgg actgacgtgg ctgatcgtcg ccgctatgcg gggatggact ccgtcggcga
     5161 ggccggcggg ctcgggacag cgccgcggga agcaccggcg gggggcaacg cgggggcctg
     5221 agcctctccc agactttggc tttcttcctc ctggagccag gacgtcggga accggaccgt
     5281 gatcgtggtg ccctcaccgg gggcgctgcg gatcgcgaag gtgccgccac cgacggtcgc
     5341 ccgctcgcgc atcatcgtca tcccgtagtg cccctcgggg cccggcgcct cgacgtcgaa
     5401 cccgaccccg tcgtcacgca gggtcatctc gacctcgtcg tccttctgct cgacctgctc
     5461 gacggcgatc cagatgttcg aggccttcgc gtgcttgagg gcgttcatca cgccctcacg
     5521 ggcgttgtgg tagagcagca aggcgatcgg agccggcacg tccacctcgt cgatgtcggc
     5581 atggaaccga acgtcggact cgcgcgccat ctccgcgagg aagccacgga tcgcctctcc
     5641 gagaccggcg cgccccagtg gcgaccgatg gaggtctcgg atcagggcac ggatacgttc
     5701 ggaagtgcgg tttttcgtct ccttgatttc gagcaggagt tcttccgtgt cgtccgtctt
     5761 cccggcttcg agatgacggc gcgcgacgtc cacctggatc gagaggcgga acaggagctg
     5821 cgcgaggtcg tcgtggagga acgctgcgat ctgcatgcgt tcgtcctggc gctcctcggc
     5881 catgcggttc gacaaggcgc ggagaacgtg ctcgcgatcc tggagttcct tgtgcgcctc
     5941 ctcaagagcc ttgctacgga agaacatctg ccgggcgagc aagagtggga gtacg
//
LOCUS       DATBRK010000130         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1317143, whole genome shotgun sequence.
ACCESSION   DATBRK010000130 DATBRK010000000
VERSION     DATBRK010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317143"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /gene="mraW"
                     /locus_tag="VIH70_03825"
     CDS             <1..368
                     /gene="mraW"
                     /locus_tag="VIH70_03825"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEY8115522.1"
                     /translation="RSPIETTDELARVVASALGARRGGPHPARRTFQALRIAVNRELE
                     ELAESLPRAVEILAPGGRVVVIAYHSLEDRIVKRFFNGKEDLEVLTRKPLRPSPAEAA
                     VNPRSRSAKLRAAERSRTA"
     gene            557..898
                     /locus_tag="VIH70_03830"
     CDS             557..898
                     /locus_tag="VIH70_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115523.1"
                     /translation="MLLASTLVGSMVFGIVVVNVLLAQTSFRIGEAERRLEELSEDNL
                     ELVREQATLSAPGRISAWASRHGMRLPDDIRILHAPGSGSGEPAGADPSSGTTYPQGT
                     KGSTGKGDPPT"
     gene            895..>1908
                     /locus_tag="VIH70_03835"
     CDS             895..>1908
                     /locus_tag="VIH70_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY8115524.1"
                     /translation="MSRPPAGRLVALFTLMTFAFGAISVRLVVLQVSQAQELQDRAFD
                     QRVRTIDLPAQRGQILDRNGARLALSTPADDVYADPQLVDDPWATATRLSPLLGVGVP
                     DLVHAMTSDGTFVYLARQLGRAASDRIQRLALPGIGLLPTSKRSYPAGSLAAQVLGFV
                     GTDGVGLTGLELGYQDVLAGAPGERTNELGLSGQAIVSGVDQERAPIAGATLVTTIDR
                     DLQFQVQAALEQAVEEQGARGGTVIVMDPRTAEVLAMASNPWFDPNAFGDAPSATYRN
                     RAVTDAYEPGSTNKVITAAAAIQERAIPLGTQLTVPWTMPVGDYIIHDSQRHAPMRMT
                     LGDI"
BASE COUNT          274 a          718 c          668 g          248 t
ORIGIN      
        1 cccggtcgcc gatcgagacc accgacgagc tcgcgcgagt ggtcgcgagc gcgctggggg
       61 cgcggcgggg cggcccccac cccgcccgcc ggacgttcca ggcgctccgc atcgcggtca
      121 accgggagct ggaggagctc gccgagtccc tgcctcgggc ggtggagatc ctcgctcccg
      181 gtggccgcgt ggtcgtgatc gcctaccact ccctggagga ccgcatcgtc aagcggttct
      241 tcaacgggaa ggaggacctc gaggtcctga ccaggaagcc gctccgacct tccccggccg
      301 aggcagcggt gaacccccgg tcgcggagcg cgaagctccg cgcggccgaa cggagccgta
      361 ccgcgtgagc cagcccgcgc gccggctcga gcccgccgaa cgccgaacgc cgcggcgcac
      421 gtccgcgcgt ccctcggcgc ctgccaccgc tcgtcccact cccacccggc ggacgcagcc
      481 ggtcgcgccg cccgcacgcg agcggcccgc cggagcccgc cgcacgagac cgagccatcg
      541 ccgatcgatc gggttcgtgc tgctcgcgtc gaccctggtg gggtcgatgg tcttcggcat
      601 cgtagtcgtg aacgtcctgc tcgcccagac gtcgttccgc atcggtgagg cggagcgacg
      661 gctcgaggag ctctccgagg acaacctcga gctcgtgcgc gagcaggcca ccctgtccgc
      721 tccgggccgg atctccgcct gggccagccg gcacggcatg cgtctccccg acgacatccg
      781 cattctccac gcgcccggtt cgggttcggg ggagccggcc ggagcggacc cttcctcggg
      841 gacgacctac ccccaaggga cgaagggctc cacagggaag ggcgacccac caacgtgagc
      901 cgcccgcccg ccggacggct ggtcgcgctg ttcaccctga tgacgttcgc cttcggggcc
      961 atctcggtgc ggctcgtcgt cctgcaggtc agccaggcgc aggagctcca ggaccgcgcg
     1021 ttcgaccagc gcgtgcggac gatcgacctg ccggcgcagc gcgggcagat cctcgaccgc
     1081 aacggcgcac ggctggcgct gtcgacgccg gccgacgatg tgtacgcgga cccgcagttg
     1141 gtggacgacc cgtgggcgac ggccacgcgc ctctcgccgc tgctcggcgt cggcgtcccg
     1201 gatctcgtgc acgccatgac ctccgacggg acgttcgtct acctcgcgag gcagctcggc
     1261 cgcgccgcct ccgatcgcat ccaacgtctc gccctaccag gcatcggcct gctcccgacc
     1321 tcgaaacgct cgtaccccgc gggatcgctc gccgctcagg tgctcgggtt cgtcgggacg
     1381 gacggggtgg gtctcacggg cctcgagctc gggtaccagg acgtcctggc gggggcgccg
     1441 ggggagcgga cgaacgagct cggactctcg ggccaggcga tcgtgagcgg ggtcgaccag
     1501 gaacgcgccc cgatcgcggg cgcgaccctg gtcacgacga tcgatcgtga cctccagttc
     1561 caggtgcagg ccgcgctcga gcaggcggtc gaggaacagg gcgcccgcgg agggaccgtg
     1621 atcgtgatgg atccgcggac cgccgaggtc ctggcgatgg cgagcaaccc ctggttcgac
     1681 ccgaacgcgt tcggggacgc gccgagtgcc acgtaccgca accgggccgt gacggacgcc
     1741 tacgaacccg gttcgaccaa caaggtcatc acggccgcgg ccgcgatcca ggagcgggcg
     1801 atccccctcg gcacgcagct cacggtcccg tggacgatgc cggtcggcga ctacatcatc
     1861 cacgactcac agcggcacgc accgatgcgg atgaccctcg gggacatc
//
LOCUS       DATBRK010000131        22972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1894032, whole genome shotgun sequence.
ACCESSION   DATBRK010000131 DATBRK010000000
VERSION     DATBRK010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894032"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VIH70_03840"
     CDS             <1..1266
                     /locus_tag="VIH70_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEY8115525.1"
                     /translation="AAANPPEPASPAYYHVSSGSRNPLQYKGLYEYVKEYFERDPLPQ
                     QGRGTVKVPEWTFPGNLRVERLLRTAERAVDVADKVVTHLPKSKRMRDLVTRVDRDKG
                     RVDFVRRYSDLYGAYTEAEVIYTDDRTVELWGGLTAADRKAFPFDAAAIDWRYYLQDV
                     HCPAVTSGLRALSGKRVKPQVRIREREQLVLALFDMEGTILPSNVVESYVWSRMADLP
                     WDQWPDELASVFSRIPSYLMADRRDRGEFLRTFFRRYEGATVEGIERLIEEIVSEFML
                     QKVSAAAVRRIRDHRAAGHRTMMITAAAEPFVQPLATLFDEVIAAKLEVRDGAYTGYL
                     AEPPLVGEARGAWLRRYAELEGADLRSSYAYADSHSDLALLRAVGNPVVVSPDAALLR
                     VATRRRWPIEDWRMAGGMPRVRFPKPAVR"
     gene            1522..2739
                     /locus_tag="VIH70_03845"
     CDS             1522..2739
                     /locus_tag="VIH70_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8115526.1"
                     /translation="MTTQAIELYRSVPRYLTARAVGERLPGLLSGPLAPMRFVNRKDV
                     VPLGPGWGRVRPLLSGICGSDLATIGGKSSFYFSPLVSLPFVPGHEVVGELLDDVDDL
                     PQGTRIALEPVLSCLARGLDPCDNCTKGLTGRCDRVTTGHVSAGLQTGYCADTGGGWS
                     KMFVAHRSQLHPVPDGLSERKAVLLEPLSCAVHTALRADLEPNANVVVIGAGTVGILT
                     LLAVKELTKAGHVAVVAKHARQREWAGAFGASEIVGPKEATNAVRRATGAMKLKPERG
                     ASFLLGGADVAIDCVGSRSSLNLALRITRAGGRVVVSGIPTEGADLTPLWFRELELVG
                     AYTGGYETVGSGKNETKTHSFDLATTIATENPIDQIVGATYPLRRWREAIDHALGAGK
                     LGTLKVAFDPRLD"
     gene            2779..4365
                     /locus_tag="VIH70_03850"
     CDS             2779..4365
                     /locus_tag="VIH70_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEY8115527.1"
                     /translation="MARPGFVLEVDERTPPLLVHQGEGFRLQKFPLGSRVIYPPDSLP
                     RLRGLRNHIRHALDHPHGSEPLGELLKPGMRLTIAIDDISIPLPPMQSPDIRQLVLEE
                     VIELAARRGIEDIKLTMANSLHRRMTADEIKHAVGERVFRSFYPKDLRNHDAEDPDNL
                     AYVGKTDRGEEVEINKRAMESDLLVYVNINLVAMDGGHKSVPVGLASYRSVKHHHNVE
                     TMLHSKSYMDPRPGRSAIHDSCKRMGDLLTANGLRVFTIETTVNNDTFPDPFGFMNKR
                     EWEWSLLEQGTYLGAKKANDLGPSAAKHQIWTRLKSPYGVTGINAGETEAVHEQTLAT
                     LHRQQLVEVNGQSDIMVVGIPYLTPYNVNSILNPILVHCMGLGYLFNMYRNKPVVRPG
                     GAMIMFHPVPNEFHQVHHPSYVDFFEEVLSETTDPSTIESKYEERYATDPWYIHLYRK
                     SYAYHGVHPFYMWYWGAHGLDYLGDVIMVGGDPKTCERLGYRAASSFRDALEMAGETV
                     GRSPSITYFHVPPLAIADVR"
     gene            4365..5270
                     /locus_tag="VIH70_03855"
     CDS             4365..5270
                     /locus_tag="VIH70_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8115528.1"
                     /translation="MSWTREIREVARGWRWGRRTMTPRSALDSTPPPKIWSYPTDWAR
                     TAAGRVARDVILDAALKPIIWNETTPEVFGLDNLEGVRGPVMFISNHSSHLDASIILT
                     TLPHTWREKTATAAAKDYFFDVWWRSAFTALVYAGFPVERGAGEKATAKAKELIREGW
                     DIIVFPEGTRSTDGWLQRFRHGTSRLALDMKMPVVPIAIVGAYAAMPKGRSWPRKGRP
                     PIRVRYGRPIYPREGETHQQLSLRMQQGVAELFDEDRTSWWEAARRAAKDETPSLAGP
                     VGPGWRRTWEGSRPIRRSGKPPTWR"
     gene            5267..5872
                     /locus_tag="VIH70_03860"
     CDS             5267..5872
                     /locus_tag="VIH70_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8115529.1"
                     /translation="MTRTGGEVRRHALIEEAIRAFGREGYSGASLDQIATAVGIRKQT
                     LLYYFPTKDALLEACLQAAGQRVAEEIASALGGKDTYWDKAEAVIHAVFALAEEWPEF
                     PMFVREAGRLGADGIERFTGALDPLRQRAIAFLQDGMDAGKIRKQDPALLLFMLYTAV
                     VGSLTEASVLNAVVGEDKGRQSLRMREREVQAFVKSALQPA"
     gene            complement(5889..6569)
                     /locus_tag="VIH70_03865"
     CDS             complement(5889..6569)
                     /locus_tag="VIH70_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEY8115530.1"
                     /translation="MATKIEETAPAREPLTKGRILRAAVALADEVGVESLSMRRLAQE
                     LGVVPMALYKHVANKDELFDGMIDVVVGEIDPPIQGADWKTTMRERILSARHALLRHP
                     WASSVLESRGEPTPTVIGYMDSMMGVFLDGGFSVDLMHHAMHVMGSRILGFSQELFDD
                     TASMPAEEAAAVWAQMADAYPNIAKLIPVAVDTHEGPVVGGGCDDQFEFEFALDLVLD
                     GLERLRVG"
     gene            6877..7851
                     /locus_tag="VIH70_03870"
     CDS             6877..7851
                     /locus_tag="VIH70_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY8115531.1"
                     /translation="MKAITQDRYGEADVLEFGDVDEPVVEENDVLIRVRAAGAGPDVW
                     HIMAGKPYMARPALGWGKPKIPIRGWDVAGTVEAVGAAVTEFRPGDEVMGTAERGSFA
                     ELTVAPADKLVRKPERLTFEQAAAVPVSGTTAIQALRDKGHVKPGQSVLVIGAGGGVG
                     TFTVQIAKAMGATVTGVASTSKLDLVRSIGADDVIDYTREDFTDGSRRWDVIVDTAGR
                     RSLRELRRALTPKGTLVVVGGDGGGPWTGGFFRGMLRGPILSMFVGQTFGWLNAKATK
                     EDLQALSELIEAGSVTSVVDRTFDLAEAAGAVRYLAEGHPAGKIVVTV"
     gene            complement(7865..9361)
                     /locus_tag="VIH70_03875"
     CDS             complement(7865..9361)
                     /locus_tag="VIH70_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY8115532.1"
                     /translation="MEHAVVIAGGGPTGLMLAGELALAGVDVAIVERRTSQELAGSRA
                     GGLHARTIEVFDQRGIADRFLSEGQVAQVAGFAGVSFDISDFPTRHPYGLGLWQNHIE
                     RILADWVGELEVRIHRGRDVTGFAQDDDGVDIELSDGESLRADYLVGCDGGRSVVRKA
                     AGIGFPGSDPTTSSLIAEAEFAEEPELGIRHDALGTHAFGREEYEIRDGEVVFSDHGP
                     VRVMVTETHVGPPTEPTLQDLSEALIAVYGTDYGIHDPTWLSRFTDMSRQATVYRDRR
                     VLLAGDAAHVHYPVGGQGLNIGVQDAVNVGWKLAQVVKQTSPESLLDTYHAERHPIAA
                     RVLRNTMAQVALGRSDDRIKAVGEVVSELLGMDEPRRRFAAEMSGLGIHYDLGEGHPL
                     LGRRVPDLDLVTADGPIRVFTLLHDARPVLLNLGEPGGIDIAPWADRVQSIDATYEGA
                     WELPAIGEVAAPAAVLIRPDGYVAWVGEQTREGLADALTTWFGSPAAA"
     gene            complement(9483..9686)
                     /locus_tag="VIH70_03880"
     CDS             complement(9483..9686)
                     /locus_tag="VIH70_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEY8115533.1"
                     /translation="MRFLGDKVLQRTAWRIADQLGWETTYDAEFVALTQLQADVFVTS
                     DRDLARAASGLVETASVDALRKA"
     gene            complement(9689..9883)
                     /locus_tag="VIH70_03885"
     CDS             complement(9689..9883)
                     /locus_tag="VIH70_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115534.1"
                     /translation="MTRFVVDCDTLLRIAAGEIEVAAGHQFVAPTLVRSQALSALYQA
                     ARRGEISAADGLERVTRSTP"
     gene            9989..10537
                     /locus_tag="VIH70_03890"
     CDS             9989..10537
                     /locus_tag="VIH70_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY8115535.1"
                     /translation="MTTYVALLRGINVGGKNPIKMPELKACFEQNGFADVATYIQSGN
                     VLFAASETRNAALTDRIEEMLAKSFDYVPTVVVRSRKQMHAIVDGAPKGFGSQPAKYR
                     YDVIFLKEPLTAKAAIERVPTNPAVDTAHAGTSVLYFSRLTAKATQSRLNKIISSPIY
                     PRVTIRNWNTTTKLMSLMDERS"
     gene            complement(10547..10918)
                     /locus_tag="VIH70_03895"
     CDS             complement(10547..10918)
                     /locus_tag="VIH70_03895"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8115536.1"
                     /translation="MQKPRSVEAYFESLPDEQRQSLQKLRKTIASAAPEADEGVTYSM
                     PGFLLDGRGLVAYAALKDHYSFFPMSPEAVDAHRKELGSRVTGKGTIRFEYGERLPTA
                     LVKKVVRTRLAEATAKRAKRR"
     gene            complement(11108..12343)
                     /locus_tag="VIH70_03900"
     CDS             complement(11108..12343)
                     /locus_tag="VIH70_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8115537.1"
                     /translation="MTPEAPTAAGIDIERVFRTEYGRAVSVLVRTFGDIDLAEEAVQE
                     AFAVAVDRWPSNGAPPSPAGWIITTARNKAIDRLRRESSRDDRHAQAVLLHARNEPIE
                     EGPVQDDRLRLIFTCCHPSLAVPSQVALTLRLLGGLTTAEIAKAFLVPEPTMAQRLVR
                     AKAKIRDAGIPYRVPYEADLPERLKPVLAVVYLIFNEGYAASSGDRLVREDLCGEAIR
                     LGRLLAELMPDEPEVMGLLALMLLTESRRETRTTPDGDLVLLADQDRGRWDRELIAEG
                     QAIVRASLRRNRPGPYQLQAAINAVHSDAATAAETDWRQILRLYDRLMAMEPSPVVAL
                     NRSVAVAEVEGPAAALVLVDELPLEGFHVFHAIRAELLSRLGRDDEADEEYERAVRVA
                     ANEPERKLLERKREASRRL"
     gene            complement(12340..12693)
                     /locus_tag="VIH70_03905"
     CDS             complement(12340..12693)
                     /locus_tag="VIH70_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8115538.1"
                     /translation="MKRYLLSIYQPDGPPPADVDLETVMANVDALVEETKAAGAWVFN
                     GGLHPSDTATVLRPRGEDVLVTDGPFLEGKEHIGGFLVVDAPDLDAALDWGRKTARAI
                     GLPIEVRPFQHGDGG"
     gene            complement(12712..13308)
                     /locus_tag="VIH70_03910"
     CDS             complement(12712..13308)
                     /locus_tag="VIH70_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8115539.1"
                     /translation="MGKIVTFTHVTLDGVMQAPGRPDEDTRGEFGFGGWEVRYADEVM
                     AGVAAQGVAAGSALLFGRRTYEDFYGFWPNQPQPNPFTDVLNRSQKYVASRTLSEPLS
                     WENSSLLKGDAAESVAELKASSQEDLVVLGSGDLIQTLMRHDLIDEYTVLIHPLVLGT
                     GRRLFEDDGPPATLRLVDSKITTTGVVIATYRPDSRTG"
     gene            complement(13584..14066)
                     /locus_tag="VIH70_03915"
     CDS             complement(13584..14066)
                     /locus_tag="VIH70_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115540.1"
                     /translation="MTGKADYTDDEWNRLRRAPFVAGMAISLADPGGPIELSHETIAT
                     LKEASTPPSREQLLIEASQDISSMVNQKQNPLAGFKPESSALAGTWVVDELSAVNAIL
                     RAKATPEEAEAFRRWLLDVAKGAADAAKEGGFMGFGAVRVSEGEQGMLEKLRSTLGVS
                     "
     gene            complement(14132..14302)
                     /locus_tag="VIH70_03920"
     CDS             complement(14132..14302)
                     /locus_tag="VIH70_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115541.1"
                     /translation="MPLYLFGIFSILAIVVGVVGLRKPPRALPNIVLAATGIALGALS
                     FLMGLVSLGVGG"
     gene            complement(14369..17539)
                     /locus_tag="VIH70_03925"
     CDS             complement(14369..17539)
                     /locus_tag="VIH70_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8115542.1"
                     /translation="MAACPNCGSENANGANFCSVCGVKLGPPEAAEVRKTVTIVFCDV
                     TGSTELGERLDPESMRKVLARYFEAMRAALERHGGSVEKFIGDAVMAVFGIPRLHEDD
                     ALRAVRAAADMRAALDDLNHELERDHGVRLACRIGVNTGEVLVGAETADFGRITGDAV
                     NTAARLEAAAEPGQILIGDATHRLVRDAVRAEAIEPLSLKGKGEPVPAHRLLEVASEI
                     GQAPRSLTSTMVGRDRELDDLTRAFARAAEDRSCLLFTILGVAGEGKTRLVEEFLSTV
                     SESANVVTGRCLPYGEGITYWPVAEAVRMALGVQAFDAPEDVATRLGDVVAGDEHAAA
                     IALRLAEILGVREGVAAAEEAPWAIRRFLEILAAELPLVALWEDIHWAEPAFLDVVDH
                     IVDWSRDAPIMLVCTARPEFLDTRSDWGGGKLNATTLLLRPLDAKASAVLIDNLLGGS
                     GLPLEDSERLTEAAGGNPLFVEQMLSMMIDDGLLVRQNGAWIPASDLSSVAAPPSVAA
                     LLAARLERLTDGERRAIESAAVVGKEFYAGAVRELLPESLGDGAPDLIRSLIRKELVR
                     TDRSMVPGEEAFRFRHILIRDAAYQAIPKERRADLHERVAAWIGRVAGDRAEEQDEIV
                     GYHLEQAFRYREALGPLDDRARELGRQAAALLAAAGSRALGRYDLVAVTHLLGRASGL
                     LEPSDPARTALLPDLAFALWEGGSADESRAVMSEALERAEQLGDPQVTAHVNLMRWFL
                     QADREGAHERARSVGEQAVALFERAGDQSGLARAWQLIGSVEWDQGQAGKQLLALDRA
                     LEHAKRAGAAFETTDILLSVTAALVRGPTPVPLGIARAERIIDEYPANRGVEAYMCHA
                     LAHLRARIGLFDAAREAAERYRDFLHDTGQVISYWRGAEVLFDIEMLAGDTETASAVA
                     EEAYARLIEREDRWPYLCAFLAQARCASGRFAEAAEVAEIAASSAIAVERALGLGVLA
                     RVRARQGDAATAEELIREAVEIVEGTDFLFDRGTVQLDLGEVMELLGRGEEARAARER
                     ALEMFEQKGDLVSAARTRSLMDRDGG"
     gene            complement(17585..18187)
                     /gene="rpsD"
                     /locus_tag="VIH70_03930"
     CDS             complement(17585..18187)
                     /gene="rpsD"
                     /locus_tag="VIH70_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY8115543.1"
                     /translation="MDKVGPRHRMCRRAGVALCGRPNCPSAKRPYPPGMHGRGRKRIS
                     EYQVRLLEKQKLRHIYGVGERQMRSYYERASRKTAVTGEELIRQLETRLDAVILRLGF
                     ALTQRQARQLVSHGHVKVNGRRLDVPSAQVKPEDTIELSDKAKNFVYVREAIELTPDP
                     PPYLYRNVDQLQGTMSRYPERNEIPLPVPIEERLIIEFYS"
     gene            18440..19369
                     /locus_tag="VIH70_03935"
     CDS             18440..19369
                     /locus_tag="VIH70_03935"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEY8115544.1"
                     /translation="MDLADRVVGSALGLALGDALGAPFEFLRARNVPDPIPALERPWL
                     GEPAGSTTDDTAMARNLMRSLAARGGLDPADVLERHLEWFRSDPPDVGTLTGRVLRRI
                     AAGEDPAAAAQAIWDERGPEVSAGNSSVMYCAPLGLAYANRPERLLELSPALSALTHV
                     DGRCRTAVTAVTFAVAALVRGEPARDAARASLIAVEDLEGGEELEFLVEAVGGSRAID
                     GPDQGFCLFTAGAAFQALVRPGDVETELRRVVSLGGDTDTNAAVAGSLLGARHGVQGL
                     PTAWLERLRDAEAIRAEAEALVPLARTRGRAAP"
     gene            19431..20429
                     /locus_tag="VIH70_03940"
     CDS             19431..20429
                     /locus_tag="VIH70_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY8115545.1"
                     /translation="MDTAERIARRADRAHRRRPWLAFPHAVLKKFGDDQAGNLAALIS
                     YYGFFSLFPLMLVLVTVLGMVLQANSELQVRIQSSALANFPVIGEDIDRNVRALGGSG
                     VTVAIGVALALWAGIGVLKVMQTAMNTVWNVPFRHRPNFVKTLLRAAIMLVVLGLITI
                     AAAAAGSVGSGNDNALLSLLWVAISLLLNFLLFVLAFRILTSVDLSWGDVWLGAAIAA
                     IAWTALQALGGYIVRHQLEGSTQTYGTFATVIGLLAWIYIGAQVTLLAAEVNVVKKRR
                     LWPRAIVQPPLTDADARALASYVRQEERRPEQHVHVRIDDRAFVQPFEPGDTEPTG"
     gene            complement(20431..21447)
                     /gene="gnd"
                     /locus_tag="VIH70_03945"
     CDS             complement(20431..21447)
                     /gene="gnd"
                     /locus_tag="VIH70_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY8115546.1"
                     /translation="MQLGMVGLGRMGANLTRRLMRGGHEVVGYDVNPDSVRLLEGEGS
                     IGTGSLEDLVSKLSKPRAAWVMVPAAFAEQTTDELAELMEPNDIVIDGGNSYYRDDMD
                     RAARYAEKGVHFVDVGTSGGVFGLDRGFCLMIGGEDEVVRRLDPIFATIAPGVGAAER
                     TPGRTDANATTAESGYLHCGPPGAGHFVKMVHNGIEYGLMAAYAEGLNVLKHAGVGAG
                     EREADAETTPLRDPGYYRYDLNIPEITEVWRRGSVVASWLLDLTAAATLEDPELSSFG
                     GRVSDSGEGRWTVLAAVDEGVPVHVLSAALFDRFESRGEADFANKILSAMRQQFGGHA
                     EKKA"
     gene            complement(21447..>22972)
                     /locus_tag="VIH70_03950"
     CDS             complement(21447..>22972)
                     /locus_tag="VIH70_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY8115547.1"
                     /translation="RRVSKGDEVTERLQQMDRDRIVERIWRKDHTVWKDDPTEITDRL
                     GWLTVSDLMHERIGELEAFAEQAAADGFETAVLLGMGGSSLAPEVFVRTFGSADGSLE
                     LIVLDTTHPETTRRVADELDLRTTLFVVASKSGGTTETLSHFAFFWDRTPNGAQFVAI
                     TDPGTPLETLARERGFRALFLNPEDIGGRYSALSYFGLVPAALIGAPLHDVLDRAGEM
                     QAASEQMHPAAEIPGATLGAMIGESAGVGRDKLTLALPEEIARLGGWVEQLIAESTGK
                     EGVGIVPVVGEPLGPPDAYGRDRVFVAIGEHEALDELEHVGHPVARLRYDGLEQIGGE
                     FFRWEMATAIAGHVLGINPFDQPNVQEAKDAINAILASGSVEDPGFDDLGVLLKQVNE
                     GDYVATLAFIDRTSETEDAIERVRLAIRDRYRVATTTGFGPRFLHSTGQLHKGGPNSG
                     VFIQVVDAGRSVDLPIPGRPFTFGTLIDAQALGDLRSLRGRGRRVARVTLDQLMEVL"
BASE COUNT         3460 a         8081 c         7942 g         3489 t
ORIGIN      
        1 gccgccgcga acccgcccga gcccgcgtcg cccgcctact accacgtctc gtcggggtca
       61 cggaacccct tgcagtacaa gggtctgtac gaatacgtga aggagtactt cgagcgcgat
      121 ccgcttcccc agcagggccg cggcaccgtc aaggtccccg agtggacgtt ccccggcaat
      181 ctccgcgtcg aacgcctcct gcggaccgcc gaacgcgccg tcgacgtggc cgacaaggtc
      241 gtcacccacc tgcccaagtc caagcgcatg cgcgatctcg tcacgcgcgt ggaccgcgac
      301 aaggggcgcg tggacttcgt gcgccggtac tcggacctgt acggggcgta caccgaggcc
      361 gaggtgatct acaccgacga ccggacggtt gagctgtggg gtgggttgac ggcggccgac
      421 cgcaaggcgt tcccgttcga cgccgcggcg atcgactggc ggtactacct ccaggacgtg
      481 cattgccccg ccgtcacgtc ggggctccga gcgctctcgg gcaagcgcgt caagccgcag
      541 gtccggatcc gcgagcgcga acagctggtg ctcgcgctgt tcgacatgga agggacgatc
      601 ctgccctcga acgtcgtcga gtcctacgtc tggtctcgga tggccgacct gccgtgggac
      661 cagtggccag acgagctcgc gagcgtgttc tcgcggatcc cctcctacct gatggccgac
      721 cggcgcgacc gcggcgagtt cttgcggacg ttcttccgtc gctacgaggg cgcgacggtc
      781 gagggcatcg agcgtctcat cgaggagatc gtgtccgagt tcatgcttca gaaggtctcg
      841 gcggccgcgg tccgacggat ccgggaccac cgcgcggccg ggcatcgcac gatgatgatc
      901 acggcggcgg ccgagccgtt cgtgcagccg ctcgcgacgc tgttcgacga ggtgatcgcg
      961 gcgaagttgg aggtccgcga cggcgcgtac acgggctacc tggccgaacc gcccctggtg
     1021 ggcgaggcgc gcggggcctg gctccgccgg tacgccgagc tcgagggcgc cgaccttcgg
     1081 tcctcgtacg cgtacgccga ctcgcactcg gacctcgcgc tgctgcgggc ggtgggcaac
     1141 ccggtggtgg tctcgccgga cgcggcgttg ctgcgggtgg ccacgcgccg gcgctggccg
     1201 atcgaggact ggcggatggc gggcgggatg ccccgcgtgc ggttcccgaa gccggcggtg
     1261 cggtgagatg gggcggtggc gcgcgggtgg tgccgcgagg tcgaggttgg ggccggagtc
     1321 gaggttgggg tcggggtcgg ggtcggcgct cgagcgcgac cgtcagggtt ctgacagaca
     1381 ggatcctgac gccgggccgc cgcctcgcgc ctcgcgcgtc agggttctga cagaaaggac
     1441 cctgacgctc cccgcgcggc gaccgacccc cacctcctcc accaccccca ccaccgccac
     1501 caccccggga gcccgccgcc catgaccacc caggccatcg agctctaccg ctcggtcccc
     1561 cgttatctga ccgcccgcgc cgtcggcgag cgcctcccgg ggcttctgag cggcccgctc
     1621 gcgccgatgc ggttcgtcaa ccgcaaggac gtcgtgccgc tcgggcccgg ctggggtcgc
     1681 gttcgcccgc tgctgtccgg gatctgcggc tcggacctcg cgacgatcgg cgggaagtcg
     1741 agcttctact tctcgccgct cgtctcgctc ccgttcgtcc ccggccacga ggtcgtcggc
     1801 gagctcctgg acgacgtcga cgatctcccc cagggcaccc gcatcgcgct cgaacccgtc
     1861 ctgagctgcc tcgcccgcgg cctcgacccc tgcgacaact gcacgaaggg ccttaccggt
     1921 cgctgcgacc gcgtcaccac cggccacgtg agcgccggcc tgcagacggg atactgcgcc
     1981 gacaccggcg gcggctggag caagatgttc gtcgcgcacc gatcgcagct gcacccggtg
     2041 cccgacgggc tctccgaacg caaggcggtc ctgctcgagc cgctcagctg tgccgtccat
     2101 accgccttgc gcgcggacct cgagcccaac gcgaacgtcg tcgtgatcgg cgccggcacg
     2161 gtcggcatcc tcacgctcct cgcggtcaag gagctcacga aggccggcca cgtcgccgtg
     2221 gtcgccaagc acgcccggca gcgggagtgg gcgggcgcgt tcggcgcgag cgagatcgtc
     2281 ggtccgaagg aggccaccaa cgccgtccga cgggcgaccg gcgcgatgaa gctcaagccc
     2341 gagcgcggcg cctcgttcct gctcggcggc gccgacgtcg cgatcgactg cgtcggctct
     2401 cgctcgagcc tgaacctcgc gctccggatc acgcgcgcgg gcggtcgcgt cgtcgtgagc
     2461 ggcatcccga ccgagggcgc cgacctcacc ccgctctggt tccgcgagct cgagctcgtc
     2521 ggcgcctaca ccggcgggta cgagaccgtc ggcagcggca agaacgagac caagacccac
     2581 agcttcgacc tcgccaccac gatcgcgacc gagaacccga tcgaccagat cgtgggcgcg
     2641 acctacccgc tccgccgctg gcgggaggcg atcgatcacg cgctcggcgc gggcaagctc
     2701 gggacgctga aggtggcgtt cgatccacgg ctcgactgac ccaagaacaa gccaagaaca
     2761 agaaggagac acgacgacat ggcacgaccg ggtttcgtcc tcgaggtcga cgaacgcacg
     2821 ccgccgctcc tggtccacca gggcgaggga ttccggctgc agaagttccc tttgggctcg
     2881 cgggtgatct acccgccgga ctcgctcccc aggctccgcg ggctgcgcaa ccacatccgg
     2941 cacgcgctcg accaccccca cggctcggag ccgctggggg agctcctcaa acccggcatg
     3001 aggctcacga tcgcgatcga cgacatctcg atcccgctgc cgccgatgca gagcccggac
     3061 atccgccagc tcgtgctcga ggaggtgatc gagctcgcgg cccgccgggg catcgaggac
     3121 atcaagctga ccatggcgaa ctcgctccat cgcaggatga ccgcggacga gatcaagcac
     3181 gcggtgggcg agcgcgtgtt ccgctcgttc taccccaagg acctgcggaa ccacgacgcc
     3241 gaggaccccg acaacctcgc gtacgtcgga aagaccgaca ggggcgagga ggtcgagatc
     3301 aacaagcggg ccatggagtc cgacctgctc gtctacgtga acatcaacct ggtcgcgatg
     3361 gacggcggcc acaagtcggt gccggtgggc ctggcgagct accggagcgt gaagcaccac
     3421 cacaacgtcg agacgatgct gcactcgaag tcgtacatgg acccgcggcc gggccgcagc
     3481 gcgatccacg actcgtgcaa acggatgggc gacctgctca cggccaacgg cctgagggtc
     3541 ttcacgatcg agaccacggt gaacaacgac acgttccccg acccgttcgg cttcatgaac
     3601 aaacgggagt gggagtggtc gctgctcgag caggggacct acctgggagc gaagaaggcg
     3661 aacgacctcg ggccttcggc cgccaagcac cagatctgga cacgcctcaa gtcgccatac
     3721 ggcgtgaccg gcatcaacgc cggcgagacc gaggccgtgc acgagcagac gctagcgacg
     3781 cttcaccggc agcagctggt cgaggtcaac gggcagtcgg acatcatggt cgtggggatc
     3841 ccgtacctca cgccctacaa cgtcaactcg atcctgaacc cgatcctcgt gcactgcatg
     3901 gggctcgggt acctgttcaa catgtatcgc aacaagccgg tcgtgcgtcc gggtggcgcg
     3961 atgatcatgt tccacccggt gccgaacgag ttccaccagg tgcaccaccc ctcctacgtc
     4021 gacttcttcg aggaggtcct gtccgagacc accgatccct ccacgatcga gtcgaagtac
     4081 gaggagcgct acgcgaccga cccctggtac atccacctct accggaagag ctacgcgtac
     4141 cacggcgtcc accccttcta catgtggtac tggggcgccc acgggctcga ctacctgggc
     4201 gacgtgatca tggtgggcgg cgacccgaag acgtgcgagc gcctgggcta ccgcgcggcg
     4261 tcgagtttcc gggacgcgct cgagatggcc ggggagaccg tggggcgctc gccatcgatc
     4321 acgtacttcc acgtgccgcc cctcgcgatc gcggacgtgc gctgatgagc tggacgcgcg
     4381 agatccgcga ggtggccagg ggctggcgtt ggggacgccg aacgatgacg cctcgctcgg
     4441 cgctggattc gacgccgccc ccgaagatct ggagctaccc gaccgactgg gcccggacgg
     4501 cggccgggcg cgtcgcccgc gacgtgatcc tggacgcggc gctgaagccg atcatctgga
     4561 acgagaccac gcccgaggtg ttcggcctgg acaacctcga gggtgtcagg ggtcccgtga
     4621 tgttcatctc gaaccactcg agccacctcg acgcctcgat catcctgacg acgctgccgc
     4681 acacgtggcg cgagaagacg gcgacggccg cggccaagga ctacttcttc gacgtgtggt
     4741 ggcgctcggc gttcaccgcg ctggtgtatg cggggttccc cgtcgagcgc ggcgcggggg
     4801 agaaggcgac cgcgaaggcc aaggagctga tccgggaggg gtgggacatc atcgtattcc
     4861 cggaggggac gcgttcgacc gacgggtggc tccagcgatt ccggcacggg acgtcgcggc
     4921 tggcgctcga catgaagatg ccggtggtgc cgatcgcgat cgtgggcgcg tacgcggcga
     4981 tgccgaaggg ccgctcgtgg ccgaggaagg gccggccgcc gatccgggtt cggtacgggc
     5041 ggccgatcta cccgcgcgag ggcgagacgc accagcagct ctcgctgcgg atgcagcagg
     5101 gcgtcgccga gctgttcgac gaggaccgca cgtcgtggtg ggaggcggcg cgccgggcgg
     5161 cgaaggacga gacgcccagc ctcgccggcc ccgtcggccc cgggtggcgc cggacgtggg
     5221 agggctcgcg ccctatccgg cgcagcggca agccccctac ctggcgatga cgcgcacggg
     5281 cggggaggtc cgccggcacg cgctcatcga ggaggcgatc cgcgcgttcg gtcgcgaggg
     5341 ctactcgggc gcgagcctgg accagatcgc gaccgccgtg gggatccgca agcagacgct
     5401 cctgtactac ttccccacca aggacgcgct gctcgaggcc tgcctccagg ccgccggcca
     5461 gcgcgtggcc gaggagatcg cgtccgcgct cgggggcaag gacacctact gggacaaggc
     5521 cgaggccgtg atccacgcgg tgttcgccct cgccgaggag tggcccgagt tccccatgtt
     5581 cgtccgcgag gcgggaaggc tcggcgccga cggtatcgag cgcttcaccg gtgcgctcga
     5641 cccactccgc cagcgcgcga tcgcattcct gcaggacggc atggacgccg gcaagatccg
     5701 caagcaggac ccggccctgc tgctgttcat gctgtacacg gcggtcgtgg gttcgctcac
     5761 cgaggcgagc gtgctgaacg cggtggtcgg agaggacaag ggtcggcagt cactgcgcat
     5821 gcgcgaacgt gaggtacagg cgttcgtcaa gagcgcgctt cagccggcgt aagatcggtc
     5881 agccctccct aaccgacacg caatcgctcg agtccgtcca acacgagatc gagggcgaac
     5941 tcgaactcga actgatcgtc gcatcccccg ccgaccaccg gtccttcgtg cgtgtcgacg
     6001 gcgaccggga tcagcttggc gatgttcggg taggcgtccg ccatctgcgc ccataccgcg
     6061 gccgcttcct ccgcgggcat gctcgccgtg tcgtcgaaca gctcctgcga gaagccgagg
     6121 atccggctgc ccatcacgtg catcgcgtgg tgcatcaggt cgaccgagaa gccaccgtcg
     6181 aggaagacgc ccatcatcga gtccatgtag ccgatgaccg tcggcgtcgg ctcgccgcgc
     6241 gactcgagca cgctcgaggc ccacgggtgc cggagcagcg cgtggcgagc ggacaggatg
     6301 cgctcgcgca tcgtcgtctt ccagtccgcg ccctggatcg gcgggtcgat ctcgccgacg
     6361 acgacgtcga tcatcccgtc gaagagttcg tccttgttgg ccacgtgctt gtacaaggcc
     6421 atcgggacga cgccgagctc ctgcgcgagc cggcgcatgc tgagcgactc cacgcccacc
     6481 tcgtcggcaa gggcgaccgc ggcgcgcagg atccgcccct tggtcagggg ctcgcgcgcc
     6541 ggcgcggtct cctcgatctt ggtcgccatg cccactcctc gtccgagccc ggtccggact
     6601 ctcggctgcc cggcttgaca agtgtacgcc gtacacccta gggtggccca gcaggtgtac
     6661 ggtgtacacc tagcgagaaa gggaacaagg tggggtcgat ggagcggcag aggcgtctgg
     6721 cgaggatcgc gggctccctg tacctggggg gtggccgtgc tcgggggggt tcgccgagct
     6781 gatcgtcagc ggcgtatctg ttgaccaagg gcgtgcgggt tcgcgaagag gaaacgctcc
     6841 cgctcgcatc ggtggcatcg taggaaggag agatccatga aggcgatcac gcaggatcgg
     6901 tacggcgagg cggacgtgct cgagttcggg gacgtcgacg agcccgtggt ggaggagaac
     6961 gacgtcctga ttcgggttcg ggcggccggg gccggcccgg acgtgtggca catcatggcg
     7021 ggcaagccct acatggcccg gcccgcgctc ggatggggca agccgaagat cccgatccgg
     7081 gggtgggacg tcgcggggac ggtcgaggcg gtcggcgccg cggtgaccga gttccggccg
     7141 ggcgacgagg tgatgggcac ggccgaacgc ggctcgttcg ccgagctcac ggtagccccg
     7201 gccgacaagc tcgtccgcaa gcccgaacgg ctcacgttcg agcaggcggc ggcggtgccg
     7261 gtctcgggca ccacggccat ccaggccctg cgcgacaagg ggcacgtgaa gcccggacag
     7321 tcggtgctgg tgatcggcgc cggcggcggc gtggggacgt tcacggtgca gatcgccaag
     7381 gccatgggcg ccacggtgac cggcgtggcg agcacgtcga agctcgacct cgtccgctcg
     7441 atcggcgcgg acgacgtgat cgactacacg cgcgaggact tcaccgacgg gtcgcggagg
     7501 tgggacgtga tcgtcgacac ggccgggcga cgctcgctgc gggagctccg ccgcgcgctc
     7561 acgccgaagg gcacgctcgt ggtcgtcggc ggcgacggag gcggcccgtg gaccggcggc
     7621 ttcttccgcg ggatgctccg cgggccgatc ctgtcgatgt tcgtcggtca gacgttcgga
     7681 tggctcaacg cgaaggcgac caaggaggac ctgcaggcgc tctcggagct gatcgaggcc
     7741 ggctcggtca cgtcggtcgt cgaccggacg ttcgacctcg ccgaggcggc cggcgcggtc
     7801 cggtacctgg ccgagggtca cccggccggc aagatcgtcg tcaccgtgta gcggtcgcgg
     7861 tgtgttacgc cgcagcgggc gatccgaacc acgtggtgag cgcgtcggcg agcccttccc
     7921 gggtctgctc tcccacccag gccacatatc cgtcgggtcg gatcaacacg gcggcggggg
     7981 cggcgacttc cccgattgcc ggaagctccc acgcaccctc gtacgtggca tcgatcgact
     8041 gaacccgatc tgcccacgga gcgatgtcga tgccgccggg ctcaccgagg ttgaggagca
     8101 ccggtcgcgc atcgtggagg agagtgaaga cacgaatcgg gccgtcggcg gtgaccaggt
     8161 cgagatcggg cacgcggcgt ccgagcagcg ggtgtccctc gccgaggtcg taatggatgc
     8221 ccagaccgga catctccgcg gcgaatcgtc tgcgaggctc gtccatgccg aggagctcgg
     8281 aaacgacctc gcccacggcc ttgatgcggt cgtccgagcg gccgagcgcg acctgcgcca
     8341 tcgtgttgcg cagcacgcgg gcggcgatcg ggtggcgctc ggcatggtag gtatccagga
     8401 ggctctccgg cgacgtctgc ttgacaacct gggccaactt ccatcccacg ttcaccgcat
     8461 cctgcacacc gatgttgagg ccctgtccac ccaccggata gtgcacgtgt gcggcgtcac
     8521 cggccagaag aacgcgtcgg tcgcggtaga ccgtggcctg ccgcgacatg tcggtgaacc
     8581 tggagagcca ggtgggatcg tggatcccgt aatcggtccc gtagacggcg atgagcgcct
     8641 cgctgagatc ctgcagggtg ggttcggtcg ggggtccgac gtgcgtttcg gtcaccatga
     8701 cccgcaccgg gccgtggtcc gagaagacga cctcgccgtc gcggatctcg tactcctccc
     8761 gaccgaaggc gtgggtgccg agggcgtcgt ggcggatgcc tagttcgggc tcctcggcga
     8821 actcggcctc ggcgattagg ctgctcgtgg tcggatccga tcccgggaac ccgatgcccg
     8881 ctgccttccg gacgacgctg cgtcctccgt cgcagccgac gagatagtcc gcgcgtaacg
     8941 actcgccgtc ggacagctcg atgtcgacgc cgtcgtcgtc ctgcgcgaag ccggtcacgt
     9001 cacgtccgcg atggatccgc acctcgagct cgccgaccca gtcggccagg atgcgctcga
     9061 tgtggttctg ccacagcccg agtccgtagg ggtgccgggt ggggaagtcg ctgatgtcga
     9121 agctgacccc ggcgaacccc gcgacctgag ccacctgtcc ctccgagagg aaccgatccg
     9181 cgatcccgcg ctgatcgaag acctcgatgg ttcgtgcgtg caggccgccg gcgcgcgagc
     9241 cggcgagctc ctggctggtg cgccgctcga cgatggcgac gtcgacgccc gccaacgcca
     9301 gttcgcccgc cagcatcagc cctgtcggac ctcccccggc gatcaccacc gcgtgctcca
     9361 ttccgcgact cctttccgtg ggttcgggcg aacgaactgg cgattgtgcg gcatgaccca
     9421 ggggttgccg caagcccccg ggtgcgctcc ataatggagg tggaggggag tgggtactcc
     9481 ctctaagcct tgcggagcgc gtcgactgac gccgtctcga cgaggccgga ggctgcccgc
     9541 gcgagatctc ggtcggacgt gacgaagacg tcagcctgca gctgcgtcag ggccacgaac
     9601 tcagcgtcgt aggtcgtctc ccaccccagc tggtccgcga tcctccaggc cgttcgttgc
     9661 agcaccttgt cgccgaggaa gcgcaccttc agggagttga tcgtgtgacc cgctcgagcc
     9721 catcggcggc ggagatctca ccccgccggg ccgcctgata cagcgccgac agcgcctgcg
     9781 agcgaaccag cgtcggcgcg acgaactggt gcccggctgc gacctcgatc tcgccggccg
     9841 cgatccggag caacgtgtca cagtcgacga cgaaccgcgt cacgcagcag acgctacacc
     9901 ctgccccgcg cttagagggc gtctgtcgat cgacatcggt aggaaccgct gcatgcgacg
     9961 tcttgccgcc tcgtactctt gaccttcgat gacgacctac gtcgcgctcc tccgcgggat
    10021 caacgtcggc gggaagaacc cgatcaagat gcccgagctc aaggcgtgct tcgagcagaa
    10081 cgggttcgcg gacgtcgcca cgtacataca gagcgggaac gtgctgttcg ccgcgtccga
    10141 gacccggaac gccgcgctga ccgatcggat cgaggagatg ctcgcaaagt cgttcgacta
    10201 tgtccccacg gtggttgtcc gcagccggaa gcagatgcat gcgatcgtag acggcgcgcc
    10261 gaaggggttc ggcagccaac cggccaagta ccggtacgac gtcatcttcc tcaaggagcc
    10321 gctcacggcg aaggccgcga tcgagagggt gccgacgaac ccggccgtcg acacggccca
    10381 cgccggcacg agcgtcctgt acttctcgag gctgaccgcg aaggcgacgc agagccggct
    10441 caacaagatc atctcgtcgc cgatctaccc gagagtgacg atccgcaact ggaacacgac
    10501 caccaagctc atgtcgttga tggacgagcg ctcctgacgc cttgggttac ctccgcttgg
    10561 ctcgcttggc ggtcgcctcg gccaggcgcg ttcgaaccac cttcttcacg agcgccgtcg
    10621 gcagccgctc accgtactcg aacctgatcg tccccttccc ggtcacgcgc gatccgagct
    10681 ccttgcgatg ggcgtctacc gcttccgggc tcatcgggaa gaagctgtag tgatccttca
    10741 gggccgcgta ggcgaccaag ccgcgaccgt cgagcaggaa acccggcatg ctgtacgtga
    10801 ccccctcgtc ggcctcgggc gcggcggagg cgatcgtctt ccgcagtttc tggagcgact
    10861 gtcgctgttc gtccggcagg gactcgaagt acgcctcgac gctcctcggc ttctgcatgg
    10921 ttcgcctcct cacaccgtgg tgggggaggc tagcgggtca ggccccgctc gccacgggct
    10981 gcacctgact agccgatcgg gcgccgccgc gatcggccag gacgcctact acctgtttct
    11041 cagcgcggtc gagatcgcga tgctgctgct gatcgtcggc tatgcgtgga ggtggccccg
    11101 gcagcactca tagcctccgc gaggcctccc gtttgcgttc gaggagtttg cgctcgggct
    11161 cgttggcggc gactcggacc gcgcgttcgt actcctcgtc cgcctcgtcg tcgcgtccca
    11221 ggcgcgagag gagctcggcg cggatcgcat ggaatacatg gaatccttcg agcggcagct
    11281 cgtccacgag cacgagcgcc gccgccggcc cctcgacctc ggcgacggcg acggaccgat
    11341 tgagggccac gaccgggctc ggttccatcg ccatcagccg gtcgtacagc cgaaggatct
    11401 ggcgccagtc cgtctccgcc gcggtcgccg cgtcgctgtg gacggcgttg atcgcggcct
    11461 ggagctgata cggacccggc cggttccgcc gcaggctcgc ccggacgatg gcctggcctt
    11521 ccgcgatcag ctcgcggtcc cagcggccac ggtcctggtc cgcgaggagc acgaggtcgc
    11581 cgtccggggt cgtgcgggtc tcgcgccgcg actcggtgag cagcataagc gcgagcagcc
    11641 ccatcacctc gggctcgtcg ggcatgagct cggctaggag ccggccgaga cggatcgcct
    11701 cgccgcagag atcctcgcgg acgaggcggt cgcccgaact ggcggcgtat ccctcgttga
    11761 agatcaggta gacgacggcg agcacgggtt tgagccgctc cgggaggtcg gcctcgtacg
    11821 gcactcggta ggggatcccc gcatcgcgga tcttcgcctt cgcgcggacg aggcgttgcg
    11881 ccatcgtcgg ctcgggtacg aggaacgcct tcgcgatctc cgcggtggtg agcccgccga
    11941 gcagccgaag cgtcagcgcg acctgcgacg gcacggcgag cgacgggtgg cagcacgtga
    12001 agatcaggcg cagccggtcg tcctgcacgg gaccctcctc gatcggctcg tttcgggcgt
    12061 gcaggagcac cgcttgggcg tgtcggtcgt cgcgggacga ctcgcggcgg aggcggtcga
    12121 tcgccttgtt ccgcgccgtc gtgatgatcc atcccgccgg gctcggtggc gcgccgttcg
    12181 acggccatcg atcgaccgcg acggcgaacg cctcctgcac ggcctcctcc gcgaggtcga
    12241 tgtcgccgaa cgttcgaacg aggaccgaca cggcccgacc gtattcggtg cggaacacac
    12301 gttcgatgtc gatgccggcg gcggtcggag cctccggcgt cacccgccgt ccccgtgctg
    12361 gaacggacgg acctcgatcg gcaggccgat cgctcgagcg gtcttgcggc cccaatcgag
    12421 ggcggcgtcg agatcgggtg catcgacgac caggaacccg ccgatgtgct ccttgccctc
    12481 gaggaagggg ccgtcggtca cgaggacgtc ctcgccccgg ggccgcagca ccgtcgccgt
    12541 gtcggacgga tggagaccgc cgttgaagac ccacgcgccg gcagctttcg tctcctcgac
    12601 cagcgcgtcg acgttggcca tgaccgtctc gaggtcgacg tccgccggcg gcggcccgtc
    12661 cggctggtag atgctgagca ggtagcgctt cattcggatc cctcctcgag gctacccggt
    12721 ccgagagtcc ggccggtagg tggcgatgac cacgccggtc gtcgtgatct tcgagtcgac
    12781 cagacgcagc gtggcgggcg gtccgtcgtc ctcgaacagc ctgcgcccgg tccccagcac
    12841 gagcgggtgg atcagtacgg tgtattcgtc gatcaggtcg tggcgcatca gcgtctggat
    12901 caggtcgccg ctcccgagca ccacgagatc ctcctgcgag ctcgccttca gctcggccac
    12961 ggactcggcg gcgtcgccct tcaggagcga ggagttctcc caggagagag gctcgctcag
    13021 ggtccgcgac gcgacgtact tctgcgatcg gttgagcacg tcggtgaacg ggttcggctg
    13081 tggttggttg ggccagaagc cgtagaagtc ctcgtacgtg cgccggccga acaggagcgc
    13141 gcttccggcg gccacaccct gggccgcgac gcccgccatg acctcgtcgg cgtaccggac
    13201 ctcccaccct ccgaacccga actcgccgcg cgtgtcctcg tcgggtcggc ctggcgcctg
    13261 catgacgccg tcgagcgtga cgtgcgtgaa ggtgacgatc ttgcccatgg tgcctcctcg
    13321 tatcgatcga cccggtcggc gatgggccgc gcgggtgctt gggtgctctc accctctaca
    13381 cgaatgcgaa ccgctcacat cgacgcgaga ggagcctttg atcttggagg actacgaacg
    13441 ggtcggagaa cgacgctcga tcagcaggat gaccgcgtag gccgcgcgag tgtgccaggt
    13501 cgacgggtgg gggcggccgt ctaatgccct caccctcgct cggatctccg cgacccgacg
    13561 cgacgtgacg ccgggagtct gactcagctc acgccgagcg tcgagcgcag cttctcgagc
    13621 atcccttgct cgccctcgct gacccgcacg gcgccgaacc ccatgaaccc tccctccttc
    13681 gccgcgtcgg ccgccccctt ggctacgtcg agcagccagc gacggaacgc ctccgcctct
    13741 tccggggtcg ccttcgcgcg gaggatggcg ttcacggccg agagctcgtc caccacccac
    13801 gtgccggcga gcgccgagct ttcgggcttg aacccggcca ggggattctg cttctgattg
    13861 accatcgacg agatgtcctg cgacgcctcg atgagcagct gctcgcggga cggcggcgtg
    13921 gaggcttcct tcagggtcgc gatcgtctcg tgggacaact cgatcggtcc gcccggatcc
    13981 gccagcgaga tcgccattcc cgcgacgaac ggggcgcgcc tgaggcggtt ccactcatcg
    14041 tcggtgtagt cggccttgcc agtcatcgtg cgctcctttc ccgatccgag ggcgtgcttc
    14101 cctggtcata ggctctcatc catcctgccg tctagcctcc gacccccaac gacacaaggc
    14161 ccatgaggaa cgacagcgcg ccgagtgcga tgcccgtcgc agccagcacg atgttcggga
    14221 gggcgcgtgg cggcttcctc aaacccacga caccaacgac gatcgcaagg atcgagaaga
    14281 tgccgaagag atacagcggc acggagagca gcccgaggac gaacgccccg accgcatagc
    14341 cgttcctcgg cgagctgggc agcggtgctc agcccccatc acgatccatc agcgagcggg
    14401 ttcgggccgc cgagaccagg tcgcccttct gttcgaacat ctcgagcgcg cgctcacgtg
    14461 cggcgcgcgc ttcctcgccg cggccgagca gctccatcac ctcgccgagg tcgagctgca
    14521 cggtcccgcg atcgaacagg aagtccgtgc cctccacgat ctcgaccgct tcccggatca
    14581 actcctcggc cgtcgcagcg tctccctggc gcgctcgcac ccgcgcgagc acgccgaggc
    14641 cgagcgcgcg ctcgacggcg atggcgctcg aggcggcgat ctcggcgacc tcggcggcct
    14701 ccgcgaaccg acccgacgca caccgggcct gggcgaggaa cgcgcagagg tagggccacc
    14761 gatcctctcg ctcgatgagg cgcgcgtagg cctcctcggc cacggcgctg gcggtctccg
    14821 tgtccccggc gagcatctcg atgtcgaaca ggacctccgc cccgcgccag taactgatca
    14881 cctgcccggt gtcgtggagg aagtcgcggt agcgctccgc cgcctcccgc gcggcgtcga
    14941 acaggccgat ccgggcccga agatgagcga gggcgtggca catgtacgcc tccacccctc
    15001 ggttcgccgg gtactcgtcg atgatgcgct cggcccgagc gatcccgagc ggaaccggcg
    15061 tcggccctct gacgagcgcg gcggtcacgc tgagcaggat gtctgtcgtc tcgaacgcgg
    15121 ccccggcccg cttggcatgc tcgagcgctc ggtcgagagc gaggagctgc ttgccggcct
    15181 gcccctgatc ccactcgacc gagccgatca attgccatgc gcgggcgagg ccagattggt
    15241 ccccggcccg ctcgaacagg gccaccgcct gctcgccgac ggatctcgcc cgttcgtgtg
    15301 cgccctcccg atccgcttgg aggaaccagc gcatcaggtt gacgtgcgcg gtcacctgag
    15361 gatcgccgag ctgctcggct cgctcgagcg cctcggacat gaccgccctc gactcgtcgg
    15421 ccgatccgcc ctcccacagc gcgaacgcga ggtcggggag gagggcggtc cgggcgggat
    15481 ccgagggttc caacaagccg gaggctcgcc cgagcaggtg ggtgaccgcg accagatcgt
    15541 accggccgag cgcacgcgaa ccggccgcgg ccagcagtgc cgccgcctgc cggcccagct
    15601 cgcgagcccg gtcgtcgagc ggcccgagcg cctctcggta ccggaacgcc tgctcgaggt
    15661 ggtaaccgac gatctcgtcc tgctcctcgg cgcgatcgcc ggccacgcgc ccgatccacg
    15721 ctgcgaccct ctcgtgcagg tcggcgcgtc gttccttggg gatcgcctga tacgccgcgt
    15781 cgcggatcag gatgtggcgg aagcggaacg cctcctcgcc cggcaccatc gagcgatccg
    15841 tccgcacgag ctccttgcgg atcagcgaac ggatcaggtc gggtgcgccg tcgccgagag
    15901 actcgggcag cagctcccgc acggcgcccg catagaactc cttgccgacc acggcggcgg
    15961 actcgatcgc gcggcgctcg ccgtcggtca ggcgctccag gcgggcggcg agcagcgccg
    16021 cgacggaggg cggcgccgcg accgagctga ggtcactggc cgggatccag gcgccgttct
    16081 gccggaccaa caacccgtcg tcgatcatca tcgagagcat ctgctcgacg aacaatgggt
    16141 tgccgccggc cgcttcggtc aagcgctcgg aatcctccag cggcaggccg gagcctccca
    16201 gcaggttgtc gatcaggacg gccgatgcct tcgcgtcgag cggtcggagc aacagcgtcg
    16261 tggcattcaa cttgccgccg ccccaatccg agcgggtgtc gaggaactcc ggacgcgcgg
    16321 tgcagacgag catgatcggt gcatcccgcg accagtccac gatgtgatcg acgacgtcga
    16381 ggaacgccgg ctcggcccag tggatgtcct cccacagcgc gaccagcggg agttcggcgg
    16441 cgaggatctc gaggaaccgg cggatcgccc acggagcctc ctcggcggcg gcgacgccct
    16501 cgcggacccc gaggatctcg gccagccgaa gagcgatcgc cgcggcgtgc tcgtcgcccg
    16561 cgacgacgtc gccgagccgg gtggcgacgt cctcgggtgc gtcgaacgcc tgcacgccca
    16621 gcgccatgcg aaccgcctcg gcgaccggcc agtacgtgat gccctcgccg taggggagac
    16681 accggccggt cacgacgttg gcggactcgc tcaccgtcga gaggaactcc tccacaaggc
    16741 gcgtcttccc ctcgccggcg acgcccagga tcgtgaacag gaggcagctg cgatcttcgg
    16801 cggcgcgggc gaacgcgcgc gtgaggtcgt cgagctcacg gtcgcggccc accatggtcg
    16861 aggtcagcga gcgtggcgcc tgcccgatct cggaggcgac ctccagcagt cggtgcgccg
    16921 gaaccggctc ccccttcccc ttgagcgaga gcggctcgat cgcctcggcc cgaaccgcat
    16981 cgcggacgag gcggtgggtc gcgtcgccga tcaggatctg cccgggctcg gcggcggcct
    17041 ccaggcgcgc ggcggtgttg acggcatcgc ccgtgatgcg gccgaagtcg gcggtctcgg
    17101 cgcccacgag cacctcgccg gtgttgacgc cgatccggca cgccaggcgg acgccgtggt
    17161 cgcgctcgag ctcgtggttg aggtcgtcga gcgccgcccg catgtcggcg gcagcccgga
    17221 cggcgcgcag cgcgtcgtcc tcatgcagcc gcgggatccc gaacacggcc atcaccgcgt
    17281 ccccgatgaa cttctcgacc gagccgccgt ggcgttcgag cgccgcgcgc atcgcctcga
    17341 agtaacgggc cagcaccttg cgcatcgact cggggtcgag tcgctcgccg agctcggtcg
    17401 agcccgtgac gtcgcagaac acgatcgtga ccgtcttgcg gacctcggcc gcctcgggcg
    17461 ggccgagctt gacgccgcac accgagcaga agttcgcccc atttgcgttc tccgagccgc
    17521 agttcggaca ggccgccacg gccggagtct aggccgctcc ggggagcacg agcaggttcc
    17581 gggactagga gtagaactcg atgatcagcc gctcttcgat cggaacgggg agcgggatct
    17641 cgttgcgctc ggggtagcgg gacatcgtgc cctggagctg gtcgacgttg cggtacaggt
    17701 acgggggcgg gtcgggggtg agctcgatcg cctcgcgcac gtagacgaag ttcttggcct
    17761 tgtccgagag ctcgatcgtg tcttccggct tgacctgcgc gctggggacg tcgaggcggc
    17821 ggccgttcac cttcacgtgg ccgtggctga cgagctggcg cgcctgacgc tgggtgagcg
    17881 cgaacccgag ccggaggatc acggcgtcca gacgggtctc gagctggcgg ataagctcct
    17941 cgccggtgac ggcggtcttg cggctcgcgc gctcgtagta cgagcgcatc tggcgctcgc
    18001 cgaccccgta gatgtggcgg agcttctgct tctcgagcag gcgaacctgg tactcggaga
    18061 tgcgcttgcg cccccgcccg tgcatgccgg gggggtaggg gcgcttggcc gacgggcagt
    18121 tcgggcggcc acacagggcc acgccggcgc ggcggcacat ccgatgacga ggtccgacct
    18181 tgtccattgc gaccaggaag gttaccagcg gggtggttgg gggccagggg acgttcgggt
    18241 cgcccggggg gaggggggat cgttcgtggg gcggccgtcg gtgttctgtc tgtcaggacc
    18301 ctgacggcga agtgccgaaa agcccacgcc gcacctgggt tctgtctgtc agaaccctga
    18361 cggagcgctc gagcgaggca ccccacctcc gccaacgagc gcgcgctcct tcacgcgccg
    18421 agtacgctcg gcgccccgga tggacctcgc cgatcgcgtc gtgggcagcg ccctcgggct
    18481 cgcgctcggg gacgcgctgg gagccccctt cgagttcctg cgcgcccgca acgtccccga
    18541 tccgatcccg gcgctcgagc gcccgtggct cggggagccg gcgggctcga ccaccgacga
    18601 taccgcgatg gcgcggaacc tgatgcgttc gctcgccgcc cggggcgggc tggacccggc
    18661 cgacgtgctc gaacgtcacc tggagtggtt ccgctccgat cccccggacg tgggcacgct
    18721 cacggggcgc gtcctccgcc gcatcgccgc cggcgaggac cccgcggccg cggcacaggc
    18781 gatctgggac gagcgcggcc ccgaggtctc ggccggcaac agctcggtca tgtactgcgc
    18841 gccgctcggc ctcgcctacg cgaaccgccc cgagcggctc ctcgagctct cgcccgcgct
    18901 ctcggccctc acgcacgtcg acggccggtg ccggaccgcg gtcacggccg tgacgttcgc
    18961 cgtggccgcg ctcgtccgcg gcgagcccgc ccgcgatgct gctcgcgcct cgctcatcgc
    19021 cgtcgaggac ctggaaggcg gcgaggagct cgagttcctg gtcgaggcgg tcggcgggtc
    19081 gcgcgcgatc gacggacccg atcagggctt ctgcctgttc acggccggcg ccgcatttca
    19141 ggcgctcgtc cgcccgggcg acgtcgagac cgagctgagg cgagtcgtct cgctcggcgg
    19201 cgacaccgac accaacgccg cggtggccgg ctccctcctc ggcgcccgcc acggcgtcca
    19261 ggggctgccg actgcttggc tcgagcgcct ccgggacgcc gaggcgatcc gagccgaggc
    19321 cgaggctctc gtcccgctcg cgcgaacccg gggacgcgca gcaccctgac gcgaaccgcc
    19381 aacgtttcgg gacggcccga atcggtgaaa catcgacttg aggaggtccc gtggataccg
    19441 cggaacggat cgctcgtcgg gcggaccgag cgcatcgccg tcgtccgtgg ttggcgtttc
    19501 cccacgcggt cctgaagaag ttcggcgacg accaggccgg gaacctcgcg gcgctgatct
    19561 cgtactacgg gttcttttcg ctgttcccgc tgatgctcgt cctcgtcacc gtcctcggga
    19621 tggtcctgca ggcgaactcc gagctccagg tgcggatcca gagctcggcc ctcgcgaact
    19681 tcccggtcat cggtgaggac atcgaccgca acgtgcgcgc gctcggcggg agcggtgtca
    19741 ccgtcgcgat cggcgtcgct ctggcgctgt gggccgggat cggcgtgctg aaggtcatgc
    19801 agacggcgat gaacacggtc tggaacgtcc cgttccgtca ccgtccgaac ttcgtcaaga
    19861 cgctgctgcg agccgcgatc atgctggtcg tcctcgggct catcacgatc gccgcggcgg
    19921 ccgcgggaag cgtcgggtcc gggaacgaca acgcgctgct cagcctcctg tgggtcgcga
    19981 tctccctgtt gctgaacttc ctgctgttcg tgctggcctt tcggatcctg acgtccgtgg
    20041 atctgtcgtg gggcgacgtg tggctgggag ccgccatcgc ggcgatcgcg tggaccgcgc
    20101 tccaggcgtt ggggggctac atcgtgaggc accaactgga ggggtcgacc cagacgtacg
    20161 gcacgttcgc gaccgtgatc gggctcctcg cgtggatcta catcggagca caggtgacgc
    20221 tgctggcggc cgaggtgaac gtggtcaaga agcggcggtt gtggccacga gcgatcgtgc
    20281 agcccccgct caccgatgcg gatgcgcggg ccctcgcgag ctacgtccgg caggaggagc
    20341 gccggccgga gcagcacgtc cacgttcgga tcgacgatcg agcgttcgtg cagccgttcg
    20401 aacccggcga caccgagccg acgggctgag ctacgccttc ttctcggcgt gccccccgaa
    20461 ctgctggcgc atcgccgaca ggatcttgtt cgcgaagtcg gcctcgcccc gtgactcgaa
    20521 ccggtcgaac agcgccgccg acagcacgtg caccggcacg ccctcgtcca ccgcggcgag
    20581 cacggtccac cggccctcgc ccgaatccga cacgcgcccg ccgaacgacg aaagctcggg
    20641 gtcctccaac gtggccgcgg ccgtcaggtc gagcagccac gacgccacca cgctcccgcg
    20701 gcgccacacc tcggtgatct cggggatgtt caggtcgtag cggtagtacc cggggtctcg
    20761 cagcggcgtg gtctcggcgt cggcctcgcg ttcgccggcg ccgaccccgg cgtgcttgag
    20821 cacgttcagg ccctcggcat aggcggccat gagcccgtac tcgatcccgt tgtggaccat
    20881 cttgacgaag tggccggccc ctggcggccc gcagtgcagg tagccggact cggcggtggt
    20941 cgcgttcgcg tcggtccggc ccggcgtgcg ttcggccgcc cccacgcccg gcgcgatcgt
    21001 cgcgaagatc gggtcgaggc gccgcaccac ctcgtcctcg ccgccgatca tcaggcagaa
    21061 cccgcggtcg agcccgaaca cgccgccgct cgtccccacg tccacgaagt gcacgccctt
    21121 ctcggcgtag cgcgccgcgc ggtccatgtc gtctcggtag tacgagttcc cgccgtcgat
    21181 cacgatgtcg ttcggctcca tcagctcggc gagctcgtcg gtggtctgct ccgcgaacgc
    21241 ggccggcacc atcacccagg ccgcacgcgg cttcgagagc ttgctcacga ggtcctcgag
    21301 cgacccggtc ccgatcgacc cctcgccctc gagcagccgg accgagtccg ggttcacgtc
    21361 gtacccgacc acctcgtggc cgccccgcat cagccggcgc gtgagattcg cgcccatccg
    21421 gccgaggccg accatcccga gctgcatcag agcacctcca tcagttggtc gagcgtgacg
    21481 cgggcgacgc gccgtccgcg cccccggagg gagcgcaggt cgccgagcgc ctgcgcgtcg
    21541 atcagcgtcc cgaacgtgaa gggccggccc gggatcggca ggtccaccga acgcccggcg
    21601 tcaaccacct ggatgaacac gccgctgttc gggccgccct tgtggagctg cccggtcgag
    21661 tgcaggaacc gcgggccgaa cccggtcgtg gtcgccaccc tgtaccggtc gcggatcgcg
    21721 agacgaacgc gttcgatcgc atcctcggtc tcggaggtcc ggtcgatgaa cgcgagggtc
    21781 gcgacgtagt cgccctcgtt cacctgcttg agcagcacgc cgaggtcgtc gaaccccggg
    21841 tcctcgaccg agccggaggc gaggatcgcg ttgatggcgt ccttggcctc ctgcacgttc
    21901 ggctggtcga atgggttgat cccgagcacg tggccggcga tcgccgtggc catctcccac
    21961 cggaagaact cgccgccgat ctgctcgagg ccgtcgtacc gcagccgggc gaccgggtgc
    22021 ccgacgtgct cgagctcgtc gagcgcctcg tgctcgccga tcgcgacgaa gacccggtcc
    22081 cgtccgtagg cgtcgggcgg gccgagcggt tcccctacca ccggcacgat gccgacgcct
    22141 tccttgccgg tcgactcggc gataagctgt tccacccagc cgcccaagcg cgcgatctcc
    22201 tcgggcagcg cgagcgtgag cttgtccctg ccgaccccgg ccgactcgcc gatcatcgcg
    22261 cccagggtcg cgcccgggat ctctgccgca gggtgcatct gttcggacgc ggcctgcatc
    22321 tccccggcgc ggtcgagcac gtcgtgcagc ggcgcgccga tgagtgccgc gggcacgagc
    22381 ccgaagtacg acagagccga gtaccggccc ccgatgtcct cggggttcag gaacagcgct
    22441 cggaacccgc gctcgcgagc cagcgtctcg agcggcgtcc ccgggtcggt gatcgcgacg
    22501 aactgggccc cgttcggcgt ccggtcccag aagaacgcga agtggctgag cgtctcggtg
    22561 gtgccgcccg acttgctcgc cacgacgaac agggtcgtcc gcaggtcgag ctcgtcggcc
    22621 actcgccggg tcgtctcggg gtgcgtcgtg tcgaggacga tgagctcgag cgagccgtcc
    22681 gcggatccga acgtgcgcac gaagacctcc ggcgcgaggc tcgaaccccc catcccgagc
    22741 aggaccgcgg tctcgaaccc gtcggccgcc gcctgttcgg cgaacgcctc gagctcgccg
    22801 atccgctcgt gcatcagatc ggagaccgtg agccagccga gacggtcggt gatctcggtc
    22861 gggtcgtcct tccagacggt gtggtccttg cgccagatcc gctcgacgat ccggtcgcgg
    22921 tccatctgct gcaggcgttc ggtcacctcg tctcctttcg agacccgtcg ca
//
LOCUS       DATBRK010000132        11219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1894055, whole genome shotgun sequence.
ACCESSION   DATBRK010000132 DATBRK010000000
VERSION     DATBRK010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894055"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="VIH70_03955"
     CDS             <1..1541
                     /locus_tag="VIH70_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019062070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY8115548.1"
                     /translation="GPPWLSVVGSAAGEVVNGVPVWFVPAEDYSRHFVDFQRDQTIAD
                     IADTVDAGLRSVEHVKRATYIGTAIDQGRTSGVITAEVVNALLGESPGWQGPSSARPP
                     AMPVPFATLAGPSRGDLFDPIRTTPIHPWHVERGASFENVGQWKRPWFFAREGESMDQ
                     AVERECLAVRNAAGAMDASTLGKIEVVGADAPAFLDRMYTNRMSNLAVGSIRYGLMLG
                     LDGMVLDDGVAMRLAEDRYLVTTTTGGAALVLDRFEEWLQTEWPELRVFCTSVTEQWA
                     VVAISGPRAREVVSATGTDVDLSRKAFPFMTFRDGHIAGVPGRLARVSFSGELAYELH
                     VPAWQGPEVWEAVMAAGEPFGIEPYGTEAMHVLRAEKGYVIVGQDTDGSVTPHDLGMD
                     WIVNLSKGDFVGRRSLRRSDIVRPDRRQLVGLLPVDPEALVPEGAQLVLDDTGEIPMP
                     LAGHVTSSYRSPTLGRAFALAMLAGGRRMHGRTVYAPLPEGTLACTVTSAIPYDPEAE
                     RRDG"
     gene            1531..2049
                     /locus_tag="VIH70_03960"
     CDS             1531..2049
                     /locus_tag="VIH70_03960"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEY8115549.1"
                     /translation="MTARSPLAHRATDLDRVGSRELAFLAQVDVRVDPTTAGALGFPT
                     EPNTWTPAGDRDVLWLGPDEWLVVAEPVGAGALAEELERSLADLHRSVVDVSANRVVL
                     ELEGSSRHDLLSSACPIDLHPRSWSDGRCAQSVFGAAQVLLQERRGTTRLFVRPSFAA
                     YVVDLLLAVAPA"
     gene            2070..2546
                     /locus_tag="VIH70_03965"
     CDS             2070..2546
                     /locus_tag="VIH70_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY8115550.1"
                     /translation="MDDDTPALRALRGSDLAYEVVRTERARSAEESAGLQGIELGQLL
                     RTIVVRRGAEDYVFVLVPAGRQIDWPRLRAHIGVSRLSLPDAEEAKAATGYERGTITP
                     FGSSNSWPVIADARIPSLERVAIGGGGHGVNLHVAGHDLVAALGAEVADVTRDGAG"
     gene            2661..3050
                     /locus_tag="VIH70_03970"
     CDS             2661..3050
                     /locus_tag="VIH70_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115551.1"
                     /translation="MRRGWAIAAAILIVLVTAGIAIGAYNAGVDEGIRRAADSEQVVQ
                     VVGGYGHGYGFFPFGFFLFPLFIFGTIFLVSRAFRGPRGWGHDHGRGYGPWSEEGRAR
                     FEDRASDWHRRQHEQVSDQESTGPPSA"
     gene            3137..3817
                     /locus_tag="VIH70_03975"
     CDS             3137..3817
                     /locus_tag="VIH70_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8115552.1"
                     /translation="MTTVLVVEDEIEIARVVRDYLVNAGFEVIVVGDGGSAIASVRSA
                     KPDLLVLDLGLPGRDGLDVAREIRRWSNTPIVMLTARGDETDRVVGLELGADDYVVKP
                     FSPKELVARIRAVLRRTRAAERGAEVLRAADVQIDTGKMRVSVAGASVELTPTEFQLL
                     ATLAREPGRVFTRSQLLDAVHGVAIESYERAIDAHVKNIRRKIEPEPASPRYLLTVHG
                     VGYRFADA"
     gene            3810..4913
                     /locus_tag="VIH70_03980"
     CDS             3810..4913
                     /locus_tag="VIH70_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8115553.1"
                     /translation="MPEFGRWRGDPPPWWPTEESWPPQGGEWSGMRGRFVRRIAIGFG
                     IFFLFFVFLVGWLGAAFGGRWFDGGDRHGGFFPWFPFLPILLVVGFIALGRAIRRTAR
                     PIGEVMDAAEKITDGDLSARAPVYGPSDIRNLAGAFNRMAERIETNERKRRDLLADVA
                     HELRTPLAVIRGRVEGMRDGLYEAEGEHLELIERETDVMARLLEDLQLLSNAEAGALR
                     LHRERLEPSELVETAADAFATPAGEGGLELKTEVGTGLPSLDVDRVRIGEVLANLLAN
                     AVRHTPPGGTVTLEASTNPQGVAFAVTDTGEGIRADELAHVFDRFARSPESRGAGLGL
                     AIAKSLVQAHGGTISAESEPGRGTTIRFVLPAG"
     gene            complement(4916..6088)
                     /locus_tag="VIH70_03985"
     CDS             complement(4916..6088)
                     /locus_tag="VIH70_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY8115554.1"
                     /translation="MAVIDNPGILLYTRIRKSPYFYASREHGVARYSVYNHTYHPRHY
                     GDPVAEYWHLVNRVTLWDVGVEKQIQIRGPDAFDLTNTIVPRDLHKCAVNECKYVFIT
                     GPDGGILNDPVLLRVAEDEFWLSLADSDVNLYALGIAAARGMDVTVREIDVAPVQIQG
                     PNAKKVLADLFGERVLEIPYYGLMQGELDGMDVVVSRTGYTGEVGYEIYLRDASQHGV
                     RLWNLVLEAGRPHGLEVIGPCHIRRIEGGILAFGADMWYEHNPFEVGYHYSWMVDLEQ
                     EADFMGKEALKRIKSEGVRRKLVGVDIEGEPLGSYIDNEMVDVFPVSVGGNEVGRVTS
                     ACFSPRLEKNIGFAMLPIGHTELGTTLEVETPVSGKVKASVVQMPHWDPTKEIPKQ"
     gene            complement(6130..8601)
                     /locus_tag="VIH70_03990"
     CDS             complement(6130..8601)
                     /locus_tag="VIH70_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8115555.1"
                     /translation="MRDRARTVVVGAGIVGASAAYHLAELGERDVLVLDQGRLFETGG
                     STTHAPGLAFQTNGSRMMCRIAQESVALYATLEHDGLPTWYGVGGIEVATSEARMREL
                     KRRLGFARSYGLEGAELLTPAEAAGKVPLLDPSTILGAYHVPSDGIAKGVRIAAALGR
                     KAEQLGVAFEGGVAVTGFEILDGRVVGVRTDHGDVECERVLLSAGIWGPSLGALAGVP
                     VPLLAVQHQLVWTDPIPELEGDAREVAHPILRHQDMAMYFRHRQDHYAVGNYRHEPIA
                     TPQSQLRGPGDAPLMPSLMPFTPADFDVAEAEAARLLPALKGRMRPADPERSINGMFS
                     FTPDAGSIVGESAKVRGFWLCEAVWVTHAGGFGRHVAEWIVQGEPSIDMAEADANRFY
                     PYMTTPPFVLERGKQQYREVYDIIHPRQQMARPRKLRLSPFYERHSELGAEFVVGAGW
                     ERPEWFEANAGLVPSNAPWLRRDEWAARFWSPIEGAEHLATRTSAALFDLTAFAKFDV
                     EGPDALAYLERICANRIDRPVGTIVYTAMLTPAGGIRCDLTITRKEEELFRVITGGGS
                     GQHDLAWMRRQLGDGEQVRIAERTGSLFALGLWGPQAREILAQVSDVDVGNEAFPYMT
                     ARYVNLGEVGPVWAQRISYAGELGWELYGQFAMGDRAWELLWGAGQDLGLVAAGLGCF
                     DTLRLEKGYRFWGQDIHTEHDPFEAGLGFAVKMDKGPFQGRDALERILERGPTERLAC
                     ITLDDPTAVVLGREPIRSDGRVVGYATSAGYGYSVGRCIVYGYLPAELAVPGTPVEVE
                     YFDERLPATVAEDPLWDPKGERLRA"
     gene            complement(8651..8821)
                     /locus_tag="VIH70_03995"
     CDS             complement(8651..8821)
                     /locus_tag="VIH70_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115556.1"
                     /translation="MASDEQAPETIPADELARLRRIEEAAKKVLAEMEGRRSATTALT
                     AIAELRAAVKQP"
     gene            complement(8844..9746)
                     /locus_tag="VIH70_04000"
     CDS             complement(8844..9746)
                     /locus_tag="VIH70_04000"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8115557.1"
                     /translation="MQASIVVLACSGLVGCSAFDLDSPPPRARGSDTIVVGVSGAFAE
                     NQLVAEMYAQVLERAGYTVQRELDLRSRESSQNALESGRIDLKPEYLSSLLLFVDRNA
                     EASEDATEVAERLGGLLRSRGITLLTPSPAQDTNQFVANAETAERFDLTTMSSLAAVA
                     GQLTMGAPPECPQRAFCLPGLKTVYGIVFNDFQPLDVGGPLTVAALSNDDVQVALLFS
                     TDPRVSENGFVPLVDDRHLQDAENITPVIRSDKLNDEIRGLLDTVSARLSNDTLTDLV
                     GQVVIDGQEVPTVARGFLAANGLL"
     gene            complement(9803..11149)
                     /locus_tag="VIH70_04005"
     CDS             complement(9803..11149)
                     /locus_tag="VIH70_04005"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8115558.1"
                     /translation="MDAIERERSTSAERSPGPFGLGRLRLWFSDPSLESGFRADQFRH
                     NLGNIRFAFLAGIVLWVSWGVLLRPYMLALSDQRIDALMRLGFFIPLLVVGLGLTFMR
                     FFARAWEWVSVAIAAATLVGWVFYVSRVLTLPAEYGYVGVILITAFTYTLLRLRFVFV
                     ALITVLGIAAYLPYALTARYIVDVSLVLATLYLVSFGILGGLAAYRMERFTRALFLRE
                     RQLDQERARSDGLLLNILPQAIVEQLKASPSGRVAQAFDQVSVVFADAVGSTEQAARC
                     APEEFADSLDELFRTFDEIADRHGLEKIKTIGDAYMAVAGAPVPMREHAEAAVAMALD
                     ILTEAGAINWPSGDSIQVRVGVATGPAVAGVIGVRKFAYDLWGDTVNLANRLEEYGEP
                     GHALVSEATASQLADRYHFAPTQVMDVKGKGPTPVRVLLGRRSDVPIASPSSPGHE"
BASE COUNT         1682 a         3926 c         3919 g         1692 t
ORIGIN      
        1 aaggtccgcc gtggttgtcg gtggtcgggt ctgcggccgg cgaggtcgtc aacggcgtgc
       61 ccgtttggtt cgttccggcc gaggattact cgcggcactt cgtcgacttc caacgcgacc
      121 agacgatcgc ggacatcgcc gacacggtcg acgcggggct gcgctccgtc gaacacgtga
      181 agcgcgccac gtatatcggt accgcgatcg atcaaggccg cacgagcggg gtgatcaccg
      241 ccgaggtcgt gaacgcgctc ctcggcgagt caccgggatg gcagggaccg tcgagcgcgc
      301 ggccgccggc gatgcccgtg ccgttcgcaa ccctggccgg cccgtcccgg ggcgacctgt
      361 tcgacccgat ccgaacgacg ccgatccacc cctggcacgt cgagcgaggc gcctcgttcg
      421 agaacgtcgg gcagtggaag cggccttggt tcttcgcgcg cgagggcgaa tcgatggacc
      481 aggcggtgga gcgcgagtgc ctggcggtgc gcaacgccgc gggtgcgatg gacgcctcga
      541 cgctcggcaa gatcgaggtg gtcggggccg acgcccccgc cttcctcgat cgcatgtaca
      601 cgaaccggat gtccaacctc gcagtcggct cgatccggta cgggctgatg ctggggctgg
      661 acgggatggt cctggacgac ggcgtcgcga tgcgtctggc cgaggaccgc tacctcgtga
      721 cgacgaccac gggcggggcc gccctcgtcc tggatcggtt cgaggagtgg ctgcaaaccg
      781 agtggcccga gcttcgcgtc ttctgcacca gcgtcaccga gcagtgggcg gtcgtcgcga
      841 tcagcggccc gcgtgcgcgc gaggtcgtct cggcgacggg gaccgacgtc gacctctcgc
      901 gcaaggcctt ccccttcatg acgttccgcg acggccacat cgcgggggtc ccggggaggc
      961 tggcgcgcgt gagcttctcg ggtgagctcg cctacgagct gcacgtgccg gcctggcagg
     1021 ggccggaggt ctgggaggcc gtgatggcgg ccggcgaacc gttcggtatc gagccgtacg
     1081 ggaccgaggc gatgcacgtc ctgcgcgccg agaagggcta cgtgatcgtg ggccaggaca
     1141 cggacggcag cgtcacgccc cacgacctcg ggatggactg gatcgtgaac ctgtccaagg
     1201 gcgacttcgt cgggcgccgt tcgctgcggc gctccgacat cgtgcgtccc gaccgcaggc
     1261 agctcgtcgg gctgctcccg gtcgacccgg aggcgctcgt gcccgaaggc gcgcagctcg
     1321 tgctcgacga caccggcgag atcccgatgc cgctcgccgg ccacgtcacg tcgtcgtacc
     1381 ggagccccac gctcgggcgt gcgttcgcgc tcgcgatgct ggccgggggt cgccggatgc
     1441 atggccgcac ggtctacgcg ccgctgcccg aagggaccct cgcgtgcacg gtgacgtcgg
     1501 cgatccccta cgaccccgag gcagagcgtc gtgacggcta ggagcccgct cgcgcatcgg
     1561 gcgaccgacc tggatcgggt gggttcgaga gagctcgcct tcctggccca ggtcgacgtc
     1621 cgggtcgacc cgacgacggc gggggccctc ggcttcccga ccgagccgaa cacctggacg
     1681 cccgccggcg atcgcgacgt gctctggctc gggccggacg agtggctcgt cgtcgccgaa
     1741 ccggtcggcg ccggcgcgct cgcggaggag ctcgagcgct cgctcgcgga cctccatcga
     1801 tcggtcgtcg acgtctcggc caaccgcgtc gtgctcgagc tcgagggctc gtcgcgacac
     1861 gacctcctct cgtccgcctg tccgatcgat ctgcacccga gatcgtggag cgacgggcgg
     1921 tgcgcgcagt cggtgttcgg cgcggcgcag gtcctgctcc aggagcggcg ggggacgacg
     1981 aggctgttcg tccggccgtc cttcgccgcc tacgtcgtcg acctgctgct cgcggtcgct
     2041 ccggcctgac cggggtaggc tcgcggacgg tggacgacga cacgcccgcg ctccgcgcgc
     2101 tgcggggttc ggacctcgcc tacgaggtgg tccggaccga acgcgcgcgc tcggccgagg
     2161 agagcgccgg cctccagggg atcgagctcg ggcagttgct ccgcacgatc gtggtgcgac
     2221 gcggcgccga ggattacgtc ttcgtcctgg ttccggcagg gcggcagatc gactggccca
     2281 ggctccgcgc ccacatcggc gtcagccggc tctcgctgcc cgacgccgag gaggcgaagg
     2341 ccgcgaccgg gtacgaacgc gggacgatca cgccgttcgg ctcgtcgaac tcgtggcccg
     2401 tgatcgccga cgcccggatc ccgtcgctcg agcgcgtggc gatcggcggc gggggccacg
     2461 gcgtcaacct gcacgtggcc ggccacgacc tcgtcgcggc gctcggggcc gaggtcgccg
     2521 acgtcacgcg cgacggagcc ggctgagccc gcggaggcac gtcgaccaca cgaaccctcc
     2581 cgaacgcctt cacgagcgct tcacatccga gcgccaacat cgatggtgac ggacgagaaa
     2641 cgttcggaag gaggaacgag atgcgacgtg gatgggcgat cgccgcggcg atcctgatcg
     2701 tgctggtcac ggcggggatc gcgatcggcg cgtacaacgc cggcgtcgac gaggggatcc
     2761 gccgcgccgc ggattcggag caggtggtcc aggtggtcgg cggatacggg cacggctacg
     2821 ggttcttccc gttcgggttc ttcctgttcc cgctgttcat cttcggcacg atcttcctcg
     2881 tgagccgggc cttccgaggc ccccgcggtt ggggccacga ccacggccgg gggtacggac
     2941 cgtggtccga ggagggtcga gccaggttcg aggaccgagc gagcgactgg caccgccgcc
     3001 agcacgagca ggtgagcgac caggagagca ccgggccgcc gagcgcctga cccccgaggc
     3061 ggcatcgggt gagcgggcgg tggcggttgc caccgcccgc tcgcgtcggc aggctagcgg
     3121 cgctggaggg cgtgtcatga cgaccgtgtt ggtggtcgag gacgagatcg agatcgcgcg
     3181 ggtggtgcgc gactacctgg tcaacgccgg gttcgaggtc atcgtcgtgg gcgacggcgg
     3241 ttcggcgatc gccagcgtcc ggagcgcgaa gcccgatctg ctggtgctgg acctcgggct
     3301 ccccggccgc gacgggctcg acgtcgcacg cgagatccgc cgttggtcga acacgccgat
     3361 cgtgatgctc accgcgcgcg gagacgagac cgatagggtc gtcgggctcg agctcggcgc
     3421 cgacgactac gtggtcaagc cgttcagccc caaggagctg gtcgcgagga tccgcgccgt
     3481 cctccgacgc acccgggccg ccgaacgcgg cgccgaggtc ctgcgtgccg ccgacgtcca
     3541 gatcgacacg ggcaagatgc gcgtgagcgt ggccggtgcg tcggtcgagc tcactcccac
     3601 cgagttccag ctgctggcga ccctcgcgcg cgaacccggc cgcgtcttca cgcggtccca
     3661 gctcctcgac gccgtgcacg gcgtggcgat cgagtcctac gagcgcgcga tcgacgccca
     3721 cgtgaagaac atccgccgca agatcgagcc cgagcccgcc tcgccccgct acctcctgac
     3781 ggtccacggc gtcgggtacc gattcgccga tgcctgagtt cggccgctgg cgcggggatc
     3841 cgcctccctg gtggccgacc gaggagtcgt ggccgccgca gggcggcgag tggagcggga
     3901 tgcgcggccg gttcgtccgg cggatcgcga tcggcttcgg gatcttcttc ctgttcttcg
     3961 tgttcctggt cggctggctc ggggccgcct tcggcgggcg atggttcgac gggggcgacc
     4021 gccacggcgg cttcttcccg tggttcccgt tcctcccgat cctgctggtg gtcgggttca
     4081 tcgccttggg ccgggcgatc cggcgaacgg cgagaccgat cggcgaggtc atggacgccg
     4141 ccgagaagat caccgacggg gacttgtcgg cccgcgcgcc ggtctacggc ccctcggata
     4201 tccgcaacct ggccggcgcc ttcaaccgga tggccgagcg aatcgagacc aacgaacgca
     4261 agcgccgcga cctgctggcc gacgtcgcgc acgagctccg gacgccgctc gcggtcatcc
     4321 gcgggcgcgt cgagggcatg cgcgatgggc tgtacgaggc cgagggtgag cacctcgagc
     4381 tgatcgaacg cgagaccgac gtcatggcac gcctgctcga ggacctccag ctgctctcga
     4441 acgccgaggc gggcgcgcta cgcttgcatc gcgagcgcct cgagccgagc gagctcgtcg
     4501 agaccgccgc ggacgcgttc gcaacgccgg caggcgaggg cgggctcgag ctgaagaccg
     4561 aggtcgggac cggcctgccg tcgctcgacg tggatcgcgt ccggatcggc gaggtgctcg
     4621 ccaacctgct cgcgaatgcc gtccggcaca caccgcccgg cggcacggtc acgctcgaag
     4681 cgtcgacgaa cccccagggc gtggcgttcg ccgtcacgga cacgggcgag ggtatccgcg
     4741 cggacgagct cgcccacgtg ttcgaccggt tcgccagatc gcccgagtcg cgcggcgccg
     4801 ggctcgggct cgcgatcgcc aagagcctgg tccaggcgca cggcgggacg atctcggccg
     4861 agagcgaacc cggcaggggc acgacgatcc gattcgtcct gccggcgggc tgacgctact
     4921 gtttggggat ctccttggtc gggtcccagt gcggcatctg cacgaccgag gccttcacct
     4981 tgccggagac cggcgtctcg acctcgagcg tggtcccgag ctcggtgtgc ccgatcggca
     5041 gcatcgcgaa cccgatgttc ttctccaggc gcggcgagaa gcaggccgag gtgaccctgc
     5101 cgacctcgtt cccacccacc gacacgggga agacgtcgac catctcgttg tcgatgtagc
     5161 tgccgagcgg ctctccctcg atgtcgacgc cgacgagctt gcgccgcacg ccctcggact
     5221 tgatccgctt gagggcctcc ttccccatga agtcggcctc ctgctcgaga tcaaccatcc
     5281 acgagtagtg gtacccgacc tcgaacgggt tgtgctcgta ccacatgtcc gcgccgaagg
     5341 cgaggatccc tccctcgatg cggcggatgt gacacggacc gatcacctcg agcccgtggg
     5401 gcctgcccgc ctcgaggacg aggttccaca gccggacgcc gtgctgggag gcgtctcgca
     5461 agtagatctc gtagccgacc tcacccgtgt aaccggtgcg cgagaccacg acgtccatgc
     5521 cgtcgagctc gccctgcatc aggccgtagt acgggatctc gaggacccgt tcgccgaaca
     5581 ggtccgcgag caccttcttg gcgttcgggc cctggatctg gacgggagcc acgtcgatct
     5641 cccgtaccgt cacgtccatc cccctcgcgg cggcgatccc gagcgcgtac aggttcacgt
     5701 ccgagtccgc gagcgagagc cagaactcgt cctcggcgac ccggagcagg accggatcgt
     5761 tgaggatccc gccgtcgggg ccggtgatga acacgtactt gcactcgttg accgcgcact
     5821 tgtggaggtc gcgcgggacg atcgtgttcg tgaggtcgaa cgcgtccggc cccctgatct
     5881 ggatctgctt ctcgaccccg acgtcccaca gcgtgacgcg gttcacgagg tgccagtact
     5941 cggcaaccgg gtcgccgtag tggcgagggt ggtacgtgtg gttgtacacg ctgtagcgcg
     6001 cgaccccgtg ctcgcgggac gcgtagaagt acggcgactt gcggatccgc gtgtagagga
     6061 ggatccccgg gttgtcgatg acggccaagc tccgctcctt ccctgcgcga tccggcccga
     6121 gcgacctcgt cacgccctca gtcgttcgcc cttcgggtcc cacaacgggt cctcggcgac
     6181 cgtcgccggg agccgttcgt cgaagtattc gacctcgacc ggcgttcccg gcaccgccag
     6241 ctcggcgggc aggtaaccgt agacgatgca gcgtccgacc gagtagccgt acccggccga
     6301 cgtggcgtag ccgaccacgc gcccgtcgga tcggatcggc tccctgccca gcacgaccgc
     6361 ggtcggatcg tcgagcgtga tgcacgcgag ccgctccgtc gggccgcgct cgaggatgcg
     6421 ttccagcgcg tcgcggccct ggaacgggcc cttgtccatc ttgaccgcga agccgaggcc
     6481 cgcctcgaac ggatcgtgct ccgtgtggat gtcctgcccc cagaaccgat aacccttctc
     6541 cagtctgagc gtgtcgaagc accccagccc ggcggccacg agtcccaggt cctgcccggc
     6601 cccccacagc agctcccagg cccggtcccc catcgcgaac tggccgtaca gctcccaccc
     6661 gagctcgccg gcgtaggaga tccgctgggc ccacaccggt ccgacctcgc cgaggttcac
     6721 gtaccgagcc gtcatgtagg ggaacgcctc gttgcccacg tcgacatcgc tgacttgcgc
     6781 gaggatctcc ctggcctgcg ggccccacag gccgagcgcg aacagcgagc cggtgcgctc
     6841 ggcgatccgg acctgctcgc catcgcccag ctggcggcgc atccaggcga ggtcgtgctg
     6901 cccgctcccg ccgccggtga tcacgcggaa cagctcctcc tccttccgcg tgatcgtgag
     6961 gtcgcagcgg atcccgcctg ccggcgtcag catggccgtg tagacgatcg tgccgaccgg
     7021 tcggtcgatc cggttggcgc agatgcgctc gaggtacgcg agcgcgtcgg gaccctcgac
     7081 gtcgaacttc gcgaacgccg tcaggtcgaa cagcgccgcg ctcgtgcgcg tggccaggtg
     7141 ctcggcgccc tcgatcggcg accagaaccg cgcggcccac tcgtcccggc gcagccacgg
     7201 cgcgttcgac gggacgagac cggcgttggc ctcgaaccac tcggggcgct cccacccggc
     7261 gcccacgacg aactcggcgc cgagctccga gtggcgctcg tagaacggcg agagccgcag
     7321 cttgcgcggg cgcgccatct gctggcgcgg atggatgatg tcgtacacct cgcggtactg
     7381 ctgcttgccg cgttcgagca cgaacggtgg cgtggtcatg tacgggtaga agcggttcgc
     7441 gtcggcctcg gccatgtcga tggacggctc gccctgcacg atccactcgg ccacgtgcct
     7501 gccgaacccg ccggcgtggg tgacccacac cgcctcgcag agccagaacc cgcggacctt
     7561 ggccgactcg ccgacgatcg agcccgcgtc cggcgtgaac gagaacatgc cgttgatcga
     7621 gcgttcggga tcggcggggc gcatgcgtcc cttgagtgcc ggcaggagcc gcgccgcctc
     7681 ggcctcggcg acgtcgaagt cggccggcgt gaacggcatg agcgacggca tcaagggggc
     7741 gtcgcccggc ccgcggagct gggactgcgg cgtcgcgatc ggctcgtgcc tgtagttgcc
     7801 gacggcgtag tggtcctgtc ggtggcggaa gtacatcgcc atgtcctggt gacgaaggat
     7861 cgggtgcgcg acctcgcggg cgtcgccctc cagctccggg atcgggtcgg tccacacgag
     7921 ctggtgctgg acggcgagca gcgggaccgg cacgcccgcc aacgcaccga ggctcggacc
     7981 ccagatcccg gcgctgagca gcacgcgctc gcactcgacg tcgccgtggt cggtcctcac
     8041 accgacgacc cgaccgtcga ggatctcgaa cccggtcacg gcgaccccgc cctcgaacgc
     8101 gacgccaagc tgttcggcct tgcgccccag agcggccgcg atccgaacgc ccttcgcgat
     8161 gccgtcggag ggcacgtgat aggcgccgag gatcgtcgac gggtcgagca gcgggacctt
     8221 cccggccgcc tcggcgggcg tcaggagctc ggcgccctcg agcccgtacg agcgcgcgaa
     8281 cccgagcctg cgcttcagct cacgcatccg ggcctcggag gtcgcgacct cgatcccgcc
     8341 gacgccgtac caggtgggca ggccgtcatg ctcgagcgtc gcgtagagcg cgaccgactc
     8401 ctgcgcgatc cggcacatca tccgggagcc gttggtctgg aacgcgagcc cgggcgcgtg
     8461 ggtggtcgag cccccggtct cgaacagccg accctggtcg agcacgagta cgtcgcgctc
     8521 gccgagctcg gccaggtggt aggccgcgct cgcgccgacg atcccggcgc cgacgacgac
     8581 ggttcgagcc cgatccctca cggttgggaa gtcttcctcg ggcgccgcgg ggcggcaagc
     8641 agcggtaccg tcacggctgc ttcaccgccg cccgcagctc ggcgatcgcg gtgagcgcgg
     8701 tcgtcgccga acgccggccc tccatctcgg ccaggacctt cttcgccgcc tcctcgatgc
     8761 gccgaagacg ggcgagctcg tccgccggga tcgtttccgg ggcctgctcg tccgacgcca
     8821 tcgcgtcctc cgacgaccga gtctcagaga agaccgttcg cggccaggaa acctctggcc
     8881 accgtcggca cctcctgccc atcgatgacg acctgtccca cgaggtcggt cagtgtgtcg
     8941 ttcgacagtc gcgcactcac cgtatcgagc agaccccgga tctcgtcgtt cagcttgtcg
     9001 gaccggatca cgggcgtgat gttctcggcg tcttggagat ggcggtcgtc cacgagagga
     9061 acgaagccgt tctcgctgac gcgtggatcc gtggaaaaga gcagggcgac ctgcacgtcg
     9121 tcgttcgaga gcgcggcgac cgtcagtggg ccccccacat cgagcggctg gaaatcgttg
     9181 aagacgatcc cgtagacggt cttcagcccc gggaggcaga aggcccgttg cgggcactcg
     9241 ggcggcgccc ccatggtcaa ctggccggcc acggctgcca gggagctcat cgtggtcagg
     9301 tcgaaacgct ccgcggtttc cgcgttcgcc acgaactggt tggtgtcctg cgcaggcgac
     9361 ggcgtgagca gggtgatgcc tcgggatcgc agcagccctc ccaaccgctc ggcgacctcg
     9421 gtcgcatcct ccgaggcctc cgcgttgcgg tccacgaaca ggagcagcga cgagaggtat
     9481 tccggcttga gatcgatccg gccggactcc agggcgttct gtgagctctc tcgcgatcgc
     9541 agatccaact cccgctggac ggtgtagccc gcgcgctcca ggacctgggc atacatctcc
     9601 gccacgagct ggttttcggc gaaggcgccc gagacgccga cgacgatcgt gtcgctgcct
     9661 cgggcgcgcg gcggcggtga atcgagatcg aaggcgctgc acccgaccaa cccggagcac
     9721 gccaagacga cgatcgatgc ttgcatgatg aggcgacggg tgcgcctgat gcctggcgag
     9781 tcttctcgga tcgtcgtgag catcattcgt gaccgggcga gctcggggat gcgatcggaa
     9841 cgtcggagcg gcggcccagc aggacgcgca cgggcgtggg ccccttgccc ttcacgtcca
     9901 tcacctgcgt gggagcaaag tgatagcgat cggccagctg gctggccgtc gcttccgaga
     9961 caagggcgtg tcctggctct ccgtactcct cgagcctgtt cgcgaggttc acggtatcgc
    10021 cccacaggtc gtacgcgaac ttgcggaccc cgatcacccc ggcgacggct ggcccggtgg
    10081 cgacgccgac ccgcacctgg atcgaatcgc ccgatggcca gttgatcgcg cccgcttcgg
    10141 tcaggatgtc gagcgccatg gcgacggccg cctcggcgtg ctcacgcatg gggaccggcg
    10201 ctccggcgac ggccatgtac gcgtccccga tggtcttgat cttctccagg ccgtgccgat
    10261 cggcgatctc gtcgaacgtg cggaacagct catccagcga atcggcgaac tcctccggag
    10321 cgcatcgcgc ggcctgctcc gtggagccga ccgcgtcggc gaacaccacg ctcacctgat
    10381 cgaaggcctg cgcgacccgg ccgctggggg aagccttgag ctgctcgacg atggcctgcg
    10441 gcaggatgtt cagcagcagg ccgtcggacc gcgctcgttc ctggtcgagc tgtcgctcac
    10501 gcaggaacag cgctcgggtg aaacgttcca tccgataggc ggccaggccg cccaggatgc
    10561 cgaacgagac gaggtagagc gtcgccagca ccaggctcac gtcgacgatg taccgagccg
    10621 tcaaggcgta cgggagatac gcggcgatgc cgaggaccgt gatcagcgcg acgaagacga
    10681 accggagccg cagcagcgtg tacgtgaacg ccgtgatcag gatgaccccg acgtatccgt
    10741 actccgcagg caacgtgagc acccgcgaga cgtagaagac ccatccaacg agcgtcgcgg
    10801 ccgcgatggc gaccgacacc cactcccacg cgcgcgcgaa gaaccgcatg aaggtgagcc
    10861 ccaacccgac gaccagcagg gggatgaaga agccgagccg catcagcgcg tcgatccttt
    10921 ggtcggacag ggccagcatg tagggacgca ggagcactcc ccacgacacc cacaggacga
    10981 tcccggcgag gaaggcgaac cggatgttgc cgaggttgtg ccggaactgg tcggctcgga
    11041 atccagactc gagggatggg tcgctgaacc acagtcgcag ccgaccaagg ccgaacggtc
    11101 ccggcgaacg ttccgccgac gtggatctct ctcgttcgat cgcgtccatc acacctcttc
    11161 attccacttc gcggccggca acgatgactg ccgaaggcat gcctgatagg ggtgccttc
//
LOCUS       DATBRK010000133         3237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1463339, whole genome shotgun sequence.
ACCESSION   DATBRK010000133 DATBRK010000000
VERSION     DATBRK010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463339"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VIH70_04010"
     CDS             complement(<1..275)
                     /locus_tag="VIH70_04010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY8115559.1"
                     /translation="MDWLDWLLLVVRVVVVFFALLILVMLLIWMERKVIADMQTRVGP
                     MRAGPRGVLITLADGIKLFFKEGITPALADRPVYLLAPVIAMLPAFLA"
     gene            complement(275..2707)
                     /locus_tag="VIH70_04015"
     CDS             complement(275..2707)
                     /locus_tag="VIH70_04015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEY8115560.1"
                     /translation="MSTPRPQDEPVTLTVDGSEVVVPKGTLIIRAAENLGIEIPRFCD
                     HPFLDPIGACRQCYVAIEGQRKLFTSCTTEVAPGMVVRTQGTSDEAHRAQVANLEFLL
                     LNHPLDCPICDRGGECPLQDQAMAFGPGESRYTEPKRTYEKPIALSPLVKLDRERCVL
                     CARCTRFCDEISGDRFIELFARGAGERVSIAAGEDFRSPFSGNTVQICPVGALTAEPY
                     RFVARPFDLTTTDSVCQHCSAGCNVRVDLRRGSVVRVLARDNAHVNDAWTCDKGRFAF
                     RHADLPGRVTTPLIRDRGLEPASFDEVFAGIAEWSREGRVAFLAGGRLPDEDAYALAK
                     LARVAFGTNDLDHRRTFHGGHAELRAAASPLDTTYRDVERAKVILVAGLDAEQEVPIL
                     NLRIRKAARGGAAVFVIHPRRTRLWDVAEHTLCLPEHQTYVLERIHDGDAPEDAFEGR
                     VSQALRRAAGEAVVLVGERLTEHPLAADVALTVAQRYGARMALVPRRAGDRGALRAGV
                     HPSLLPGGRRVSDPAERAEIDGSWGSLPSEGGRSTRQILEACASREIEVLFLIGIDPL
                     RDIPDADLVRRALSNTRHVVVQSLELGSLEPFADAFLPAAAWVEREGHATDWEGRSQP
                     IHPVRGPAGVSRADWEIFAGLAEAMGRPLGFSDLGALRAEAAPLLEPRSVAARSTAWT
                     GTGRPQRLGELTLLSYPLLVDEGRLSEGADELKASLGQDPFVELHPEDADKRGLVDGG
                     RATVTTDAGEAVLDVRVTQHVAQGAAFVPFNQPGVAANELLRGSFTTAVRIEPAVAEP
                     AVAEAAVAEGAA"
     gene            complement(2704..>3237)
                     /locus_tag="VIH70_04020"
     CDS             complement(2704..>3237)
                     /locus_tag="VIH70_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY8115561.1"
                     /translation="VLGGRRLKAWTPGGSSTPMLTEEHLDVGLDFESITAAGSLLGTG
                     AIIVMDETDCIVEAARRLVQFYAHESCGKCTPCREGTWWVSRVLGRIEQGYGRSEDLP
                     LLDELGENILFKAFCALADGAVSPIQSTLKYFRAEYEAHIAERRCPFTGVGPGHETPT
                     DPGDVTANDAGLTEALT"
BASE COUNT          460 a         1197 c         1155 g          425 t
ORIGIN      
        1 gcgaggaacg cgggcagcat cgcgatcacc ggtgcgagca ggtagaccgg ccggtccgcg
       61 agcgccggcg tgatcccctc cttgaagaag agcttgatcc cgtcggcgag cgtgatcagc
      121 acgccccgcg gacccgcgcg catcggcccg acgcgggtct gcatgtcggc gatcaccttg
      181 cgctccatcc agatcaggag catcaccagg atcagcagcg cgaagaagac caccaccacg
      241 cggaccacga gcagcagcca gtcgagccag tccatcaggc cgccccctcg gcgaccgcgg
      301 cctccgcgac cgcgggctcg gcgaccgcgg gttcgatccg gacggccgtc gtgaacgatc
      361 ctctcaacag ctcgttcgcg gcgaccccgg gctggttgaa cggcacgaac gccgcgccct
      421 gcgcgacgtg ctgcgtgacg cggacgtcga gcaccgcctc acccgcgtcg gtcgtcacgg
      481 tcgcgcgccc gccgtccacg agcccgcgct tgtcggcgtc ctctgggtgg agctcgacga
      541 acgggtcctg ccccagggag gccttgagct cgtccgcccc ctcggacagc cgcccctcgt
      601 ccacgagcag cgggtacgac agcagggtga gctcgcccag ccgctgaggg cgtccggtcc
      661 cggtccacgc ggtcgagcgc gcagccaccg agcgcggctc gagcaggggt gcggcctccg
      721 cccggagcgc cccgaggtcc gagaacccga gcgggcgccc catcgcctcg gccagacccg
      781 cgaagatctc ccagtccgcc cgcgacacgc cggcggggcc gcggacgggg tggatcggct
      841 gcgaccggcc ctcccagtcg gtggcatggc cctcgcgctc gacccacgcg gcggcgggga
      901 ggaaggcgtc ggcgaacggc tcgagcgacc cgagctcgag cgactgcacc accacgtgtc
      961 gcgtgttgga gagcgctcgc cggaccaggt cggcgtccgg gatgtcccgg agcggatcga
     1021 tgccgatcag gaacaggacc tcgatctcgc ggctcgcgca cgcctcgagg atctggcggg
     1081 tcgaacgtcc gccctccgac ggaaggctcc cccacgagcc gtcgatctcc gcgcgctcgg
     1141 ccggatcgct cacacggcgg ccgccgggca ggagcgatgg gtggacgccg gcgcggaggg
     1201 ccccgcgatc gccggcgcgc cgcgggacca gcgccatccg ggccccgtac ctctgggcga
     1261 ccgtcagcgc gacgtcggcg gcgagcggat gctcggtcag gcgttccccc accagcacga
     1321 cggcctcgcc ggccgcccgg cgcagggcct ggctcacacg gccctcgaac gcgtcctcgg
     1381 gcgcgtcgcc gtcgtggatc cgttccagca cgtacgtctg gtgctcgggg aggcagaggg
     1441 tgtgttcggc gacgtcccac aggcgggtcc gacgagggtg gatcacgaac acggcggccc
     1501 ccccacgggc ggccttgcgg atcctcaggt tcaggatcgg cacctcctgc tcggcgtcca
     1561 gccccgcgac caggatcacc ttcgcgcgct ccacgtcgcg gtaggtcgtg tccagcggcg
     1621 acgccgcggc ccgcagctcc gcgtggccgc cgtggaaggt cctccggtgg tccaggtcgt
     1681 tggtcccgaa cgccacccgc gcgagcttgg cgagcgcgta cgcgtcctcg tccgggaggc
     1741 ggccccccgc gaggaacgcg acgcggccct cgcgcgacca ctcggcgatg cccgcgaaga
     1801 cctcgtcgaa cgaggcgggt tcgagcccac gatcgcggat cagcggcgtc gtgacgcgcc
     1861 ccggcaggtc ggcgtgccgg aacgcgaacc gccccttgtc gcaggtccag gcgtcgttca
     1921 cgtgcgcgtt gtcgcgggcg aggacgcgca cgaccgaacc ccggcgcagg tcgacgcgga
     1981 cgttgcaccc ggcgctgcag tgctgacaga ccgagtccgt cgtcgtgagg tcgaaggggc
     2041 gggcgacgaa gcggtacggc tcggccgtga gcgcccccac ggggcagatc tgcaccgtgt
     2101 tgccggagaa cggggaccgg aagtcctcgc ccgcggcgat cgagacgcgt tcgcccgcgc
     2161 cccgcgcgaa cagctcgatg aaccggtcgc cgctgatctc gtcgcagaac cgggtgcagc
     2221 gcgcgcacag gacgcatcgc tcgcgatcga gcttgacgag cggtgagagc gcgatcggct
     2281 tctcgtacgt gcgcttgggc tcggtgtacc ggctctcgcc gggtccgaac gccatcgcct
     2341 ggtcctgcag cgggcactcg ccgccgcgat cgcagatcgg gcagtcgagc gggtggttca
     2401 gcagcaggaa ctccaggttc gcgacctggg cgcggtgggc ctcatccgac gtgccctggg
     2461 tccgcaccac catgcccggc gccacctcgg tcgtgcagga ggtgaacagc ttgcgctgcc
     2521 cctcgatcgc cacgtagcac tggcggcaag cgccgatcgg gtccaggaac gggtggtcgc
     2581 agaaccgggg gatctcgatc ccgaggttct cggccgcccg gatgatcagc gttcccttcg
     2641 gaacgacgac ctccgacccg tccaccgtga gcgtgaccgg ctcgtcctgc ggccgcgggg
     2701 tgctcacgtg agcgcctcgg tcaggccggc gtcgttcgcg gtcacatcgc ccgggtcagt
     2761 gggggtctcg tgccccggcc cgacgccggt gaacgggcag cggcgttccg cgatgtgggc
     2821 ctcgtactcg gcgcggaagt acttcagggt cgactggatc gggctcaccg cgccgtccgc
     2881 gagcgcgcag aaggccttga acaggatgtt ctcgccgagc tcgtccagga gggggaggtc
     2941 ctcggaccgt ccgtacccct gctcgatccg gccgagcacc cggctcaccc accacgtgcc
     3001 ctcgcggcac ggcgtgcact tgccgcacga ctcgtgggcg tagaactgca cgagccggcg
     3061 agccgcctcg acgatgcagt cggtctcgtc catcacgatg atcgcgccgg tccccaggag
     3121 cgagccggcg gccgtgatcg actcgaagtc cagcccgacg tcgaggtgct cctcggtcag
     3181 catcggggtc gaggaaccac cgggcgtcca ggccttgagc cgccgcccgc cgagcac
//
LOCUS       DATBRK010000134         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_19381, whole genome shotgun sequence.
ACCESSION   DATBRK010000134 DATBRK010000000
VERSION     DATBRK010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19381"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..422
                     /locus_tag="VIH70_04025"
     CDS             126..422
                     /locus_tag="VIH70_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115562.1"
                     /translation="MSGFQMGQLAGFGAASNGLVILGTSKDMAYRLTTSVPSLRALWT
                     CPGRGSTNDSPAPYWMPVQLSPSPEAFFESSPEVDGLCSATWSFFRTAYGRRTT"
     gene            910..1320
                     /locus_tag="VIH70_04030"
     CDS             910..1320
                     /locus_tag="VIH70_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115563.1"
                     /translation="MRLGEQFLCAGCAMEVLRESVEDFESVTEAGSVEPIETLTWGDR
                     ESVLAHGLEVGRMVREGTMQLVDAATAHHRALARSIRRARDTAECVRRIEASAVLFNV
                     WAFSFDARLEDLERQNRALRLLVRELEREVAEFH"
     gene            1347..1727
                     /locus_tag="VIH70_04035"
     CDS             1347..1727
                     /locus_tag="VIH70_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115564.1"
                     /translation="MGLGNEECWAVETSRDGQKWRSLGRAWKSPGEPVLMHVSVRFVR
                     YRQLVPAETTEWSEPLESEGDLPMTMVRMEDRARQDLWPGDEHRGLPMLLPGGETGRL
                     QAFEHAPDGSSWRYTLEFRGAREG"
     gene            complement(1765..>2274)
                     /locus_tag="VIH70_04040"
     CDS             complement(1765..>2274)
                     /locus_tag="VIH70_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEY8115565.1"
                     /translation="RFGRQVQGGLWVSREWETLRALSEAGLPVPRPIARTDDAILMGY
                     IGDLELAAPQLRSVRPDAAQAQDLFDEVVRAIERMLSINVIHGDLSAYNLLVWDGRVT
                     IIDLPQAIDPRTNRHAHALLERDVTRISEHFERLGLRSSPDRLATDLWTAWQFADLVP
                     EELRTGIEM"
BASE COUNT          344 a          736 c          824 g          370 t
ORIGIN      
        1 caccggtcga gctcgcgggt tcgtcggagg cgtgagccgg taaaagggag ccgtgggttc
       61 ggcgttctcg cgtcgagccc cgtaagaccg agggcccggc aaccatgtgg ctgccgggcc
      121 gttcggtgtc cggattccag atggggcagt tggcagggtt cggggcggcg agcaacgggc
      181 tggtcatcct cggaacgtcg aaggacatgg cgtacaggct gacgacgtcg gtcccttcgt
      241 tgcgggcgct gtggacgtgc cctggccggg gatcgacgaa cgactcgccc gccccgtatt
      301 ggatgccggt gcagctctcg ccgtcgccgg aggcgttttt cgaatcgtcc cccgaggtcg
      361 acggtctgtg ctcggcgacc tggtcgttct tccgaacggc ctacgggcgg cggacgacct
      421 agcccggaaa ggggattgac cggccgaggc cgagcttgct tggttggaaa cgcaacctgc
      481 cctcgtggga ggtcgtcatg gatgacgaac cgacgctcga atcgcaggca aggcaacgga
      541 catccgtccg cgcgttcaga cctccgatcc tctcctcgat cgttcctcgg gcggtcgcga
      601 tgcatcgcgt cgcccccgct ctgtcggtcg aacgccccag ctgatcctgc ccgggcgaac
      661 acctcgaccg atcccgcgtc cgacccacga agcgttcggg ttggccgcat gagaaaggag
      721 cgaacgatgc aaccggaatc gcgatcgaca tccaccggcg ccctcgtggt cagttgcgat
      781 cgctgcggcg aacgagcgag cgtccgaagc ggcaggaaaa tcctgtgccc gacgtgcgcg
      841 gcagcgggcg ccgatgcctc gcctgacgac gccctcatcc tgtgtgatct ctgtgatcgg
      901 gagtcgctgg tgcgactcgg tgagcagttc ctgtgcgcag gttgcgccat ggaggtcctg
      961 cgcgagagcg tggaggactt cgagtcggtg accgaggccg ggtccgtgga gccgatcgag
     1021 accctcacct ggggcgatcg tgagtccgtg ctggcgcacg ggctggaggt gggccggatg
     1081 gtccgggagg ggacgatgca gctcgtcgat gcggcgaccg cccatcatcg ggcgctcgcg
     1141 agatcgatcc ggcgagctcg agacacggcc gagtgcgtac ggcgtatcga ggcgagcgcg
     1201 gtcctgttca acgtgtgggc gttctcgttc gacgcccggc tcgaggatct cgaacgacag
     1261 aaccgggcac ttcgcctcct ggtccgggag ttggagcgag aggtggccga gttccactag
     1321 tccgtacccc gtcgtagggt ttgcccatgg gtctggggaa cgaggaatgc tgggcggtcg
     1381 agacctctcg ggacgggcag aagtggcggt ccctcgggag ggcctggaag tcgccgggcg
     1441 agccggtcct gatgcacgtc tcggttcggt tcgtccggta ccgccagctc gtaccggccg
     1501 agacgaccga gtggagcgag ccgctcgaat ccgagggcga cctcccgatg acgatggttc
     1561 ggatggagga ccgagctcgg caggacctgt ggcctggtga cgagcaccgc gggctcccga
     1621 tgctcctacc cgggggcgag accgggaggc tccaggcgtt cgagcacgcg cccgacggct
     1681 cgagctggcg ctacacgctc gagttccgcg gtgcgcgaga ggggtaggac cgcgggtccg
     1741 gacgatccca gacccgcgga cccgtcacat ctcgatcccg gtccgcagct cctcgggcac
     1801 taggtcggcg aactgccacg cggtccacag gtccgtggcg agccggtccg gcgacgatcg
     1861 cagcccgagc cgttcgaagt gctcgctgat gcgagtcacg tcgcgctcca gcagcgcgtg
     1921 ggcgtgccgg ttcgtgcgcg ggtcgattgc ctgcgggagg tcgatgatcg tcacgcgccc
     1981 gtcccacacg agcaggttgt atgcggacag gtcgccgtgg atcacgttga tggacagcat
     2041 ccgctcgatc gcgcgcacga cctcgtcgaa cagatcctgc gcctgggcgg cgtccggcct
     2101 gaccgagcgg agctgcggcg cggccagctc gaggtcgccg atgtagccca tcaggatcgc
     2161 gtcgtcggtc ctcgcgatcg gtcgcgggac gggcagcccc gcctccgaga gcgctcgcag
     2221 cgtctcccac tcgcggctca cccacaaccc gccctggacc tggcggccga accg
//
LOCUS       DATBRK010000135        10354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2331144, whole genome shotgun sequence.
ACCESSION   DATBRK010000135 DATBRK010000000
VERSION     DATBRK010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10354
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331144"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..247
                     /locus_tag="VIH70_04045"
     CDS             2..247
                     /locus_tag="VIH70_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115566.1"
                     /translation="MEIVVDSGSGVLTYEIEATKAGRRVEVATSRGVIEVSEVTRNGE
                     PVRSGRFMADRVVALVEHPATPPSVRARRVLRGQTQL"
     gene            296..910
                     /locus_tag="VIH70_04050"
     CDS             296..910
                     /locus_tag="VIH70_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8115567.1"
                     /translation="MYLDVFSDNSFETNCWLLGADGTDEVVVVDPGFSPERVRAMLDA
                     EGRRPVAAFATHGHFDHIGSAAVFCGDDLPFYIHEADVAALTDPQGWGAGSRTPPVPV
                     KDVRTVVDGDVLTFAGLTIEVVHTPGHTPGSVCLSTDALLLSGDLVFAGAIGRSDFPN
                     SSPADMERSLDRFLELPDPTDVLPGHGPRTTVGRERERNPFLGR"
     gene            911..2185
                     /gene="hisS"
                     /locus_tag="VIH70_04055"
     CDS             911..2185
                     /gene="hisS"
                     /locus_tag="VIH70_04055"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY8115568.1"
                     /translation="MELQPPRGTDDLLPPASDAMLGLYGAAHRTARLYGFRYVETPTY
                     EHTELFERTSGETSDVVTKEMYTFEDKGGRSLTLRPEQTAGIVRAYLAHAHDLPTPFK
                     AYHVSPQFRHGRPQAGRLREFRQFGVETIGSAAPKADVEIVALGDRFLRARGLDGIEL
                     RVNSIGDAECRPAYREKLIAYLEPHKAELDEDCRTRLRTNPLRVFDCKVDGQTPLVLG
                     APLISDNLCQACAEHFAAFRNGLDAAGIAFVHDPRLVRGLDYYTRTTFEIVSTALSRA
                     QGSVCGGGRYDGLAEVLGGPPTPGVGFALGLDRLLLALREEGAALPGQIGLAGFVVSI
                     GPEATEVGQRLVDELRNAGIPAATTFEDRPLKAQLRLADRSGAAYALILGEREVAAET
                     AVLKSLADGEQLEVPIGEVANWLSRTDWAAER"
     gene            2202..3998
                     /gene="aspS"
                     /locus_tag="VIH70_04060"
     CDS             2202..3998
                     /gene="aspS"
                     /locus_tag="VIH70_04060"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY8115569.1"
                     /translation="MSDLGTAMRTHACGELRDEHTGERVALCGWVWRRRDHGGVSFLD
                     LRDREGLVQLVFHPEDAPEAHAAAQHLGAESVIRVSGEVRRRPPGTVNPELPTGEIEV
                     VVDELEVLAEAETPPFVIEDRIEASEELRLRYRYLDLRRPEMTKVLAMRNAINRIVRD
                     EMESRGFLEVETPLLGRSTPEGARDFLVPSRLWPGTFYALPQSPQQLKQLLMVAGQDR
                     YYQIVRCLRDEPTRADRSFEFTQLDVEMSFVDEEDVFAVIEPLYARIVREIQGVEVAV
                     PFPRLTYDEMMDRYGSDKPDLRYGMELANVGEAFASTGFRAFASVLESGGLIKAFSAP
                     GGAELSRRELDELVQVARSRGAAGLVWIAVEGEGIRSPVEKHLSPGEIEGVRRATGAG
                     PGDLILIVADKPDRTNVALDGLRRHLADRLGLIPEGEWRFCWYSEPPLFDWSEEEGKW
                     VSNHHPFTSPLTEDLAPETAKARAYDLTLNGFELGGGSIRIHRPDVQRRVFGVLGLLP
                     EQTEEQFGHLLRAFRYGVPPHGGIAMGIDRLVMLMAGKDTIRDVTAFPKAQSGADPLT
                     GAPARATEEQLRELGIAIVAPTTHDEEAEPDG"
     gene            3991..4386
                     /locus_tag="VIH70_04065"
     CDS             3991..4386
                     /locus_tag="VIH70_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEY8115570.1"
                     /translation="MVELTESDIEMLTDTNFATFVTVNPDGSPHATITWVDAADGHVL
                     VNTAEGRVKHRNCAANPKVAITVMRNGDAYDWISITGNVVAFETGEQAERHIDELSHR
                     YDGHGYTYTPGQVREILKIRPRRIIRYKD"
     gene            4393..5682
                     /locus_tag="VIH70_04070"
     CDS             4393..5682
                     /locus_tag="VIH70_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY8115571.1"
                     /translation="MADVPLAARMRPRTFEEFVGQSHLIGPGKALTQLLEGGHLPSIV
                     LWGPAGTGKTTLAYLLADAVGAHMVQLSAVSSGVADARKVIEQASGGLFRTVLFVDEV
                     HRWSKAQQDVLLPAVEEGTVTLVGATTENPYFSLVTPLLSRCILLQLEPLAPQELRAL
                     LEHALADEDRGLGKQGVEVSDEAFKHLVEVAGGDARVALTGLEAAVLAARSAGEDSVT
                     RERAADAVQKKAIVYDRQGDAHYDVISAFIKSIRGSDADAALFWLARMLEAGEDARYI
                     ARRMVVHASEDIGLADPRALMIAVAAAQAVEYVGLPEAQLNLAEAAIYLARAPKSNSV
                     IAALGRAQKDATSSDPVPLHLKEPGHPGLKKLGFGRDYKYPHDYPDHRVDQEYLPARF
                     SGNRYYEPSGEGEETDPPSDGGAPDAVEGTAEEAPPV"
     gene            5770..6174
                     /locus_tag="VIH70_04075"
     CDS             5770..6174
                     /locus_tag="VIH70_04075"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEY8115572.1"
                     /translation="MIAALDGGDVALIVLSAFWGLLVLFLCVVLINTFRVLESTKILI
                     DVMREETVPLLREVKGSLERTNRELDRVDVMLEATGEIVGRVRRISGLVEEVVSGPLV
                     RLISIGVGVRKATSRFGGKGRKAGKGDTKGSA"
     gene            6171..6398
                     /locus_tag="VIH70_04080"
     CDS             6171..6398
                     /locus_tag="VIH70_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115573.1"
                     /translation="MRRLFWVALGLGAGAASAILATRFVRRQTAKVAPANLAREARGG
                     IMDLAKLVSDSLAEGRAAMHQKEDELRGNPR"
     gene            6447..6908
                     /locus_tag="VIH70_04085"
     CDS             6447..6908
                     /locus_tag="VIH70_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115574.1"
                     /translation="MIRSRTAALGVLAVAALAACGSRTSPADMEAMMRPTPNARSTAA
                     PTAIALRGTFVKLTGFPCHRSDPYVGGETVTFRGTDGSRTTIVTRSANWIGLPATSEA
                     PLGQCRQVARFTAELPPARRYVVLINDRRLPSVTLDELRAEGLRHTFRLPS"
     gene            6966..9617
                     /gene="alaS"
                     /locus_tag="VIH70_04090"
     CDS             6966..9617
                     /gene="alaS"
                     /locus_tag="VIH70_04090"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY8115575.1"
                     /translation="MEGDRIREEFLRFFEERGHRRVPSSSLIPPPESGLLLTNAGMNQ
                     FIPYFLGHARPPFPRAVTVQKVFRAVDIDLVGHTARHLSLFEMLGNFSFGDYFKAESC
                     RWGYELVTEVYGIEPDRLWVTVFRTDDEAIGIWRDIGVPAERIVRRGEEDNFWWTHAA
                     GPAGPCSEIYVDRGSRFGAEGGPEVDEERHIEIWNHVFMQDEVDDQAQIVRELPKKNI
                     DTGASVERLAVVLQDVDHAFETDLLRPLVEVAESLSGRRLGKDERDDVSLKVIGEHGR
                     ATTFLIADGVQPSNEGRGYLVRRMLRRVVSHARRLGIEHEVMPALVERTVERFGHVYP
                     ELVENRAYVEQVASSEEVRFAGTLRQGMTLFETAIEEASGAERLPGDVVFKLHDTFGF
                     PKELTGELAAEAGLEIDDERFEALMEEQRERAKRSAKNVRVEEELSTVAGEAGATEFV
                     GYQTLESDGRLLALIGLGGREPVATEGDEVRFVLDRTPFYAEAGGQVGDQGVVRAPGG
                     TITVTDAQFGPGDIIVHTGVVGSGEIREGEEVHGEVDADRREATARAHTATHIVHWTL
                     RHVLGDHARQAGSLVAPGRLRFDFPHHSAVPHDVLGEAEELANRRLAEDGQVTIYETT
                     MDEAKNQGAIALFGEKYGDLVRVVEVGDYSVELCGGTHVHHTGEIALVRILHEASIGA
                     GMRRVEALVGPDALREINLERDLLYAIARELGTDPKGALERARHYAERVKELESELGR
                     QAKQELRDRAQHLASGARVVEGAKLVIAAVDADADELRVLAPDVANRLEGPDGAAVAL
                     GTGRGGKALLVAASTKNLIARGVGAPALLEPAANIVGGGKGGKPNLAFSGGPKGDAFQ
                     EALDAVEPRLKELLQGDRGARGANTHA"
     gene            9614..10129
                     /gene="ruvX"
                     /locus_tag="VIH70_04095"
     CDS             9614..10129
                     /gene="ruvX"
                     /locus_tag="VIH70_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY8115576.1"
                     /translation="MSDSPPENEPENEPENEPENEPENEAENEPLAGRGRVLGLDLGD
                     ARIGVAISDEARRMAVPLGTVRTGAPADLRAIADLVREHGVTLVVVGHPLLLSGEAGE
                     RAHHAERFAAAMGEALDVPVRLQDERLSTVEAERALRESGTTGRDLRRTIDRSAATVI
                     LQAWLDTDRPV"
BASE COUNT         1563 a         3514 c         3830 g         1447 t
ORIGIN      
        1 ggtggagatc gtcgtcgact cgggctcggg cgtgctgacc tacgagatcg aggcgacgaa
       61 ggcggggcgc cgcgtcgagg tcgccacgtc gcgcggcgtg atcgaggtct ccgaggtcac
      121 caggaacggg gagccggtcc gcagcgggcg gttcatggcc gaccgggtgg tggccctggt
      181 ggagcacccc gcgacgccgc cgtcggttcg ggcccggcgg gtgctcaggg gccagaccca
      241 gctctgatcg cgttcgcgcc aggcgccgac gcggcgctcg atagactctc cccccatgta
      301 cctggacgtc ttctcggaca actcgttcga gaccaactgc tggttgctcg gcgccgacgg
      361 gaccgatgag gtggtggtgg tcgaccccgg gttctcgccc gaacgcgtcc gcgcgatgct
      421 ggacgccgag ggcaggcggc cggtcgccgc gttcgccacg cacggtcatt tcgaccacat
      481 cgggtcggcg gcggtcttct gcggcgacga cctgcccttc tacatccacg aggcggacgt
      541 cgccgcgctg accgatccgc aggggtgggg agcggggtcg cggacgccac ccgtccccgt
      601 gaaggacgtt cgaacggtcg tcgacgggga cgtgctcacg ttcgccgggc tcacgatcga
      661 ggtcgtgcac acccccggcc acaccccggg gagcgtgtgc ctgtccaccg acgccctcct
      721 gctctctggc gacctcgtgt tcgcgggcgc catcggccgg agcgacttcc ccaactcgtc
      781 gccggccgac atggaacggt cgctcgaccg gttcctcgag ctacccgatc cgaccgacgt
      841 cctcccgggc cacggtccac ggaccaccgt cgggcgcgaa cgcgagcgca accccttctt
      901 ggggcgctga gtggagctgc agccgccacg agggaccgac gacctgttgc cgccggcctc
      961 cgacgcgatg ctggggctct acggggccgc ccaccggacc gctcgcctgt acggcttccg
     1021 ctacgtcgag acgccgacct acgagcacac cgagctgttc gagcggactt cgggcgagac
     1081 ctccgacgtg gtcacgaagg agatgtacac gttcgaggac aagggcgggc gatcgctcac
     1141 gttgcgcccg gagcagacgg ccggcatcgt ccgcgcgtac ctcgcgcacg cccacgacct
     1201 gccgacgccg ttcaaggcgt accacgtctc gccgcagttc aggcacggac ggccgcaggc
     1261 cgggaggctg cgcgagttcc ggcagttcgg ggtcgagacg atcggatccg ccgccccgaa
     1321 ggccgacgtc gagatcgtcg cgctcggcga tcgcttcctc cgcgcgcgcg gcctcgacgg
     1381 gatcgagctg cgtgtcaact cgatcggcga cgccgaatgc cggccggcct accgcgagaa
     1441 gctgatcgcg tacctcgagc cgcacaaggc ggagctcgac gaggactgtc ggacgcggct
     1501 ccggactaat ccgctccgcg tgttcgattg caaggtcgac gggcagacgc cgctcgtgct
     1561 cggggcgccg ctgatctccg acaacctgtg ccaggcctgc gccgagcact tcgcggcgtt
     1621 ccgcaacggg ctggacgccg ccgggatcgc gttcgtccac gatccgcggc tcgttcgcgg
     1681 gctcgactac tacacgcgga cgacgttcga gatcgtctcg accgcgctct cgcgggctca
     1741 ggggtccgtg tgcggcggtg ggcggtacga cgggctcgcc gaggtgctcg gcgggccccc
     1801 gaccccgggg gtggggttcg cgctcggcct ggaccggctg ctcctcgccc tgcgcgagga
     1861 gggagcggcg ctccccggcc agatcgggct cgcgggcttc gtggtctcga tcggtccgga
     1921 ggcgacggag gtcgggcagc ggctcgtcga cgagctccgg aacgcgggca tccccgcggc
     1981 gacgacgttc gaggaccgac ccttgaaggc gcagctgagg ctcgccgacc gctcgggcgc
     2041 cgcgtacgcc ttgatcctcg gggaacgcga ggtggcggcg gagacggccg tcctgaaaag
     2101 cctcgcggat ggggaacaac tggaggtccc catcggcgag gtggcgaact ggctctcccg
     2161 aacggattgg gccgcggagc ggtgagggcg cgggcgctcg gatgagcgat ctcgggacgg
     2221 cgatgcggac gcacgcgtgc ggcgagctcc gggacgagca caccggcgag cgcgtcgccc
     2281 tctgcgggtg ggtgtggcgc cggcgcgacc acggcggggt gagcttcctc gatcttcggg
     2341 atcgcgaggg gctggtacag ctcgtgttcc atcccgagga cgcgccggag gcacacgccg
     2401 cggcgcaaca cctcggcgcc gagtcggtga tccgggtttc gggcgaggtc cggcgacgcc
     2461 cgccgggtac ggtgaacccc gagctgccca cgggcgagat cgaggtggtc gtggacgagc
     2521 tcgaggtgct cgccgaggcc gagacccccc cgttcgtgat cgaggaccgg atcgaggcct
     2581 cggaggagct gcggctccgc taccgctacc tcgacctgcg acgtcccgag atgaccaagg
     2641 tcctcgcgat gcggaacgcg atcaatcgca tcgtccgcga cgagatggag tctcgggggt
     2701 tcctggaggt cgagaccccg ctgctcggcc gtagcacgcc cgagggtgcc cgggacttcc
     2761 tggtcccatc gaggctgtgg cccgggacct tctacgcgct gccgcagtcg ccgcagcagc
     2821 tcaagcagct gctgatggtg gcaggacagg atcgctacta ccagatcgtc cggtgcctgc
     2881 gcgacgagcc gacgcgcgcc gaccgaagct tcgagttcac gcagctcgac gtggagatgt
     2941 cgttcgtcga cgaggaggac gtcttcgcgg tgatcgagcc cctgtacgcg cggatcgtgc
     3001 gcgagatcca gggcgtcgag gtcgccgtgc cgttcccgag gctgacctac gacgagatga
     3061 tggaccggta cggttcggac aagcccgacc tgcggtacgg gatggagctc gcgaacgtcg
     3121 gcgaggcttt cgcgagcacg gggttccgag cgttcgcatc ggtgctggaa tcgggcgggc
     3181 tcatcaaggc cttctcggca cccggcggcg ccgagctgtc ccgcagggag ctcgacgaac
     3241 tggtgcaggt ggcgaggtcg cgcggggcgg cgggcctggt gtggatcgcg gtcgagggcg
     3301 aggggatccg gtcgccggtc gagaagcacc tgtcgcccgg ggagatcgag ggcgtacggc
     3361 gcgcgaccgg ggccgggccc ggcgacctga tcctgatcgt ggccgacaag cccgaccgca
     3421 ccaacgtggc gctcgatggg ttgcggcggc acctggccga ccggctcggc ctgatccccg
     3481 agggggagtg gcggttctgc tggtactcgg agccgccgct gttcgactgg tcggaggagg
     3541 agggcaagtg ggtctcgaac caccacccgt tcacctcgcc gctgaccgag gacctcgcgc
     3601 ccgagaccgc gaaggcgcgc gcctacgacc tcacgttgaa cgggttcgag ctcgggggcg
     3661 ggagcatccg gatccaccgc cccgacgtgc agcgaagggt cttcggggtg ctgggtctct
     3721 tgcccgagca gaccgaggag cagttcggcc acctgctgcg ggcgttccga tatggggtgc
     3781 ccccgcacgg aggcatcgcg atggggatcg atcggttggt gatgctgatg gccggcaagg
     3841 acacgatccg agacgtgacg gcgttcccca aggcgcagtc cggcgccgat ccactcaccg
     3901 gagcgccggc ccgcgcgacc gaggaacagc tgcgtgagct cggcatcgcg atcgtggccc
     3961 ccacgacgca cgacgaggag gcggagcctg atggttgagc tcacggagtc cgacatcgag
     4021 atgctgacgg acacgaactt cgcgacgttc gtcacggtga acccggacgg gtcgccacac
     4081 gcgacgatca cctgggtcga cgcggccgac gggcacgtcc tggtgaacac ggcggagggt
     4141 cgggtcaagc accgcaactg cgcggcgaac cccaaggtcg cgatcaccgt catgaggaac
     4201 ggcgatgcct acgactggat ctcgatcacc gggaacgtgg tggcgttcga gaccggcgag
     4261 caggccgaac ggcacatcga cgagctctcg caccggtacg acggccacgg ctacacctac
     4321 acgcccggcc aggtgcgcga gatcctgaag atccggccca ggcggatcat ccgctacaag
     4381 gactgacctc gagtggccga cgtccccctc gccgcccgga tgcgcccccg aactttcgag
     4441 gagttcgtcg ggcagtcgca cctcatcggt cctggcaagg cgctcacgca actgctcgag
     4501 ggcggccacc tgccctcgat cgtcctgtgg gggccggcgg gaacggggaa gacgaccctc
     4561 gcctaccttc tcgccgacgc ggtcggcgcc catatggtgc agctgtctgc ggtctcctcc
     4621 ggcgtggctg acgctcggaa ggtgatcgag caggccagtg gcgggctctt ccggacggtg
     4681 ctgttcgtcg acgaggtcca ccggtggtcc aaggcgcagc aagatgtcct cctgccggcg
     4741 gtcgaggagg ggacggtcac cctggtgggc gcgaccaccg agaacccgta cttctcgctc
     4801 gtcacgccgt tgctctcgcg ctgcatcctc ctgcagctcg aaccgctggc gccgcaggag
     4861 ctccgcgcgt tgctcgagca cgccctggcc gacgaggacc gcggcctggg caagcagggc
     4921 gtcgaggtct cggacgaggc gttcaagcac ctcgtcgagg tggccggcgg ggacgcccgg
     4981 gtcgcgctca ccggcctgga ggccgcggtc ctggcagcgc gttcggccgg ggaggactcg
     5041 gtcacccgtg agcgcgcggc cgacgcggtg cagaagaagg cgatcgtcta cgaccgacag
     5101 ggtgacgcgc actacgacgt gatctcggcg ttcatcaaga gcatccgtgg gtccgatgcg
     5161 gacgccgcct tgttctggct cgcccggatg ctggaggcgg gcgaggatgc gcgctacatc
     5221 gcccgtcgga tggtcgtgca cgcgagcgag gacatcggcc tggccgaccc ccgtgcgctg
     5281 atgatcgccg tggcggccgc gcaggccgtc gagtacgtgg ggctgccgga ggcccagctc
     5341 aacctcgcgg aggccgccat ctacctggct cgcgcaccga agtccaacag cgtgatcgcc
     5401 gcgctcggcc gggcgcagaa ggacgcgacc tcctccgacc ccgtgccgct ccacctcaag
     5461 gagcccgggc acccggggtt gaagaagctc gggttcggca gggactacaa gtatccgcac
     5521 gactaccccg accaccgggt cgaccaggag tacctgcccg cgcggttctc cgggaaccgg
     5581 tattacgaac cgtcagggga gggggaggag accgatccgc cgtccgacgg cggcgcgccc
     5641 gacgcggtcg aggggacggc ggaggaggca cccccggtat gatgcgcgaa cccggaaccc
     5701 gcccgcgtgc gggccacgcg gcgggcccgg aggcggaccg gtggcggaga ggaaggcggc
     5761 gacgtgggca tgatcgcagc gctcgacggt ggtgacgtcg cactcatcgt gctctctgcg
     5821 ttctgggggc tgctcgtcct gttcctgtgc gtcgtcctca tcaacacctt ccgcgtgctg
     5881 gagagcacga agatcctgat cgacgtgatg cgcgaggaga cggtcccgct gttgcgcgag
     5941 gtgaaggggt ccctggagcg cacgaaccgt gagctcgacc gcgtcgacgt catgctcgag
     6001 gcgaccgggg agatcgtcgg ccgggtccgg cgcatctcgg ggctcgtcga ggaggtcgtc
     6061 tccgggcccc tggtcaggct catctcgatc ggggtcgggg tccgcaaggc tacgagccgg
     6121 ttcggcggca agggccgcaa ggccggcaag ggcgacacga agggttcggc gtgaggcggt
     6181 tgttctgggt cgcgctcggt ctgggggccg gggccgcgag cgcgatcctg gcgacgaggt
     6241 tcgtgcgtag gcagaccgcg aaggtcgcgc cggccaacct cgcccgcgag gcgcgtggcg
     6301 gcatcatgga cctcgccaag ctcgtgtccg actcgctcgc cgagggcagg gcggcgatgc
     6361 accagaagga agacgagctc cgcggcaacc cgcggtagcc ggccggtcga cccgcgcgga
     6421 tccacgacac gtatgcccag aacgagatga tccgttcgcg cacggccgcc ctcggggtcc
     6481 tcgccgtggc ggcgctcgcc gcctgcgggt cccgaacgtc gcccgccgat atggaggcga
     6541 tgatgcgacc taccccgaac gcgaggtcga cggcggcgcc gaccgcgatc gcgctccggg
     6601 gcacgttcgt caagctcacg ggcttcccct gccacaggag cgatccctac gtgggcgggg
     6661 agaccgtgac cttccgaggg accgacggtt cgcggaccac gatcgtgacc cggagcgcga
     6721 actggatcgg actcccggca acgtccgagg ccccgctcgg ccagtgccgt caggtggccc
     6781 gattcaccgc cgagctcccg ccggcgaggc gctacgtcgt cctgatcaac gaccggcggc
     6841 tcccgtcggt cacgctcgac gagttgcgcg ccgaggggct acgccacacg ttccgccttc
     6901 cgtcctgacg ccgcccgacc ggggtcagac ggtcgggagc aggcacgcgg ctagactgtg
     6961 ccgccatgga gggtgatcgg atccgcgagg agttcctgcg cttcttcgag gagcgcgggc
     7021 accggcgcgt cccgtcctcg tcgctgatcc cgccgcccga gtccggcctg ctcctgacga
     7081 acgccggcat gaatcagttc atcccgtact tcctcggcca cgcccggccg ccgttcccgc
     7141 gtgccgtgac cgtccagaag gtcttccgcg cggtcgacat cgacctcgtc ggacacaccg
     7201 cgcgccacct gtccctgttc gagatgctcg ggaacttctc gttcggcgac tacttcaagg
     7261 ccgagtcctg ccgttggggc tacgagctcg tcaccgaggt ctacgggatc gagccggacc
     7321 ggctgtgggt caccgtgttc cggaccgacg acgaggcgat cgggatctgg cgggacatcg
     7381 gagtccccgc cgagcggatc gttcgccgcg gcgaggagga caacttctgg tggacgcacg
     7441 cggccggccc cgccggaccc tgttcggaga tctacgtgga ccgtggatcg cggttcgggg
     7501 ccgagggcgg gcccgaggtc gacgaggagc ggcacatcga gatctggaac cacgtcttca
     7561 tgcaggacga ggtcgacgac caggcgcaga tcgtgcggga gcttcccaag aagaacatcg
     7621 acacgggggc gagcgtggag cgcctggccg tcgtcctgca ggacgtggac cacgcgttcg
     7681 agaccgacct gctgcggccg ctcgtcgagg tcgccgagtc cctctcggga cggcgtttgg
     7741 gcaaggacga gcgcgacgac gtctcgctca aggtgatcgg cgagcacgga cgagcgacca
     7801 cgttcctgat cgccgacggc gtgcagccct cgaacgaggg gcgcggctac ctcgtgcgcc
     7861 ggatgctccg tcgcgtcgtc tcgcacgcga ggcggctcgg gatcgaacat gaggtcatgc
     7921 cggcgctcgt ggagcggacg gtcgagcggt tcgggcacgt gtacccggag ctcgtcgaga
     7981 accgggcgta cgtcgagcag gtcgcgagct cggaggaggt caggttcgcc ggcaccctgc
     8041 gccaggggat gacgctgttc gagaccgcga tcgaggaggc atcgggagcc gagcggctcc
     8101 ccggcgatgt cgtgttcaag ctccacgaca cgttcggctt cccgaaggaa ctcacggggg
     8161 agctcgccgc ggaggccggg ctcgagatcg acgacgaacg gttcgaggcc ctcatggagg
     8221 agcagcgcga gcgcgcgaag cgctcggcca agaacgtgcg cgtggaggag gagctctcca
     8281 cggtcgccgg cgaggcgggc gcgaccgagt tcgtcggata ccagaccctc gagtccgacg
     8341 gaaggctgct cgcgctgatc ggactcggcg ggagggagcc ggtcgcgacc gagggcgacg
     8401 aggtgcgctt cgtcctggac cggacgccgt tctacgcgga ggccggcggc caggtcggcg
     8461 accagggtgt cgtccgggcg cccggcggga cgattaccgt gaccgacgcg cagttcgggc
     8521 cgggcgacat catcgtccac accggtgtcg tcggttccgg cgagatccgc gagggcgagg
     8581 aggtccacgg cgaggtcgac gccgaccgtc gcgaggcgac cgcccgggcc cacaccgcga
     8641 cgcacatcgt ccactggacg ctgcgccacg tgctgggcga tcacgcgcgg caggccgggt
     8701 cgctcgtcgc gcccggccgt ctccggttcg acttcccgca ccactcggcg gtcccgcacg
     8761 acgtcctcgg ggaggccgag gagctggcca accgtcggct ggccgaggac gggcaggtca
     8821 cgatctacga gacgacgatg gacgaggcca agaaccaggg cgccatcgcg ctgttcggcg
     8881 agaagtacgg cgacctcgtc cgggtcgtgg aggtggggga ctactcggtc gagctctgcg
     8941 gaggcacgca cgtccaccac acgggcgaga tcgcgctcgt ccgcatcctc cacgaggcct
     9001 cgatcggcgc cggcatgcgg cgcgtcgagg ccctcgtcgg ccccgatgcc ctgcgcgaga
     9061 tcaacttgga acgcgatctt ttgtacgcga tcgctcgcga gctcggcacc gatccgaagg
     9121 gcgcgctcga gcgcgcccgc cactacgccg agcgcgtgaa ggagctcgag agcgagctcg
     9181 gcaggcaggc gaagcaagag ctgagggacc gggcgcagca tctcgcctcc ggggcgaggg
     9241 tggtggaggg cgcgaagctc gtcatcgcgg ccgtcgatgc ggacgccgac gagctccgcg
     9301 tgctcgcccc tgacgtggcg aaccgcctcg agggtccgga cggtgcggcc gtcgcgctcg
     9361 ggaccgggcg gggtggcaag gccctgctgg tggccgcgag cacgaagaac ctgatcgcca
     9421 ggggcgtggg cgcgccggcg ctgctcgaac cggcggcgaa catcgtgggc ggcggcaagg
     9481 ggggcaagcc caacctcgcg ttctccggcg gcccgaaggg ggacgccttc caggaggccc
     9541 tggacgccgt cgagccccgc ctgaaggagc tcctgcaggg ggaccggggg gctcgaggag
     9601 ccaacacgca cgcgtgagcg atagcccccc agagaacgag ccagagaacg agccggagaa
     9661 cgagccagag aacgagccgg agaacgaggc ggagaacgag ccgttggccg ggcgcggcag
     9721 ggtcctcgga ctcgatctcg gggacgcgag gatcggcgtg gcgatctcgg acgaggcccg
     9781 gcggatggcg gtgcccctcg gcaccgtccg gacgggggcg cccgccgacc tgcgggcgat
     9841 cgccgacctc gtccgagagc acggcgtgac gctcgtcgtg gtcggtcacc cgctgctgct
     9901 ctcgggagag gcgggagagc gggcgcacca cgccgagcga ttcgcggcgg cgatggggga
     9961 ggccctcgac gtcccggtcc ggctgcagga cgagcgcctg tccaccgtgg aggccgaacg
    10021 cgccctgcgg gagagcggga cgaccggtcg cgatctccgg cggacgatcg atcgctcggc
    10081 agccaccgtg atcctgcagg cctggctcga caccgatcgg cccgtctgag cccgacgcaa
    10141 cggtcgcctc ggaccgaacc cggttcccga cggggccgct atgctgcctg gccgatgcag
    10201 gacgcgcccc ctacgcagcg cgacgacgac gctcgcccgc gccaccgatc cgaacggcgg
    10261 gccaactggg gcttccgcat cttcctgatc gtcttcctgg tgctcgccgg cctggccatc
    10321 gccggcaacc gctactacgg ctggtgctcg gagg
//
LOCUS       DATBRK010000136         5959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_455602, whole genome shotgun sequence.
ACCESSION   DATBRK010000136 DATBRK010000000
VERSION     DATBRK010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455602"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VIH70_04100"
     CDS             complement(<1..474)
                     /locus_tag="VIH70_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115577.1"
                     /translation="MFVLVVLAFVVSSGHAAAALWVTLDPTSGAPGETIRVRTAGQGA
                     MAIARPGDAMSLYLRGGLPGSATEETPLEVGQITVDSGLNGVGSFVVPALPAARYEVR
                     LRCEPCAEFSFGNTLLWVADFTITAPMPSTDTEPTEDVTIPAALVALLVVVPVLLR"
     gene            complement(507..2417)
                     /locus_tag="VIH70_04105"
     CDS             complement(507..2417)
                     /locus_tag="VIH70_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2169..2171),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8115578.1"
                     /translation="MKNGLTRLTEPMVRENGGLRTATWDEALDRAAAGLREATERNAG
                     DGVGLFSCSKATNEMNFVAQKFMRAVLRSNNIDSCNRTUHAPSVAGLATVFGAGGGTS
                     SYREIEETDVIVLWGSNARETHPIFFHHLLRGVRNGARLYAVDPRRTSSAEWADTWLG
                     LDVGSDIALANAIAREIIHAGLHHKEFIGRATSDFEAYRDKVESYTLEYAERETGVPA
                     DAIRDLAHAYAGANRAMICWTLGITEHHNAVDNVLALINLALLTGHVGRYGSGVNPLR
                     GQNNVQGGGDMGALPDRLPGFQHVENDELRAKFDKAWGVTIPPQRGWHLSGMFDAMER
                     GELQALYVIGENPLQSEADQRRAAHLLSTRDFMVVQDLFLTATAELADVVLPAAAAWA
                     ECEGTVTNSERRVQRVRKALDPPGEARDDLEIIYDLARRMGSEWGSASAEKTWDEVRS
                     LSPVHAGMTYMRLEELGGIQWPCYDENHPGELFLHSRLWEDPVPGNRAPFVPVDHDPP
                     VDKLDADYPIRLTTGRRLDSYNTGVQTASYTSPMRRGETLDISPEDAERFGLTEGERV
                     CAVSRRGQVEVPIRIDPGLRPGLTFMTLHFQDDVATNLLTIDATDPKSGTAEFKATAI
                     RIEKLPEVATAR"
     gene            complement(2483..3322)
                     /gene="fdhD"
                     /locus_tag="VIH70_04110"
     CDS             complement(2483..3322)
                     /gene="fdhD"
                     /locus_tag="VIH70_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY8115579.1"
                     /translation="MSVTEPIRHSSTRARVVAVHDGIRSERTDSLATEEPLEIRVQGP
                     GQDPLQVAVTMRTPGGDFELAVGFLFTEGLIAPGDVQRVAYCDNLPGEDQRYNVVSVT
                     LARPFEAERLRRNFYANSSCGVCGKAALEDIDVRCEPVGPGPEVELGVLISLPERLRE
                     AQAVFERTGGLHAAGLFDASGSALAVREDVGRHNAVDKVVGERYLASQIPADGTILQV
                     SGRASFEIVQKAAVAGVPIVSAVSAPSSLAVEAAERFGMTLVGFVRDGRANVYSHAAR
                     VPT"
     gene            complement(3385..4599)
                     /locus_tag="VIH70_04115"
     CDS             complement(3385..4599)
                     /locus_tag="VIH70_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8115580.1"
                     /translation="MHPIRYFRPWTMWRRHDLKSAYDVAIVGGGAHGLAIAYELAKRG
                     ITRVAVLEKSYIGAGGSGRNTTIIRANYRTPEGVAFYKQALRLYEHLAQELDYNLLFS
                     QQGHLTLAHAERAVNVAHERAEVNRLLGVDSRVIYPDEIRRLCPQLDLSDRPAFPIMA
                     ALYHPPGGVIRHDAVVWAYARQADRMGVEIHEGVEVTGVRVDGGKVTGVSTSAGDVSA
                     PVVVSATAGWTSEIARMAGIRVPITTHPLQAFVTEPLKPFLHAILVSATLHVYVSQTD
                     RGEVLIGSEIEPYSSYAQDSTLNFLEVTAEHALELLPFLSRVKVMRAWGGLCDVSPDY
                     SPIMGTTEVEGFLIDGGWGTYGFKATPIVGVTMADLIHTGTVPDLIAPFSLNRFWKRE
                     LVSEIAAAAVSH"
     gene            complement(4602..5228)
                     /locus_tag="VIH70_04120"
     CDS             complement(4602..5228)
                     /locus_tag="VIH70_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115581.1"
                     /translation="MNEPIRRSPLDRSHADLGAVQDREAGWRLVAHYGDEAAERATLR
                     EGVGLGDVTARGKIDVRGSLDGALSGAGDVLVARLADDWALLLSEPGGEEVLLPKLAS
                     AAGPGAMVTDATHLFVGFALAGPDLPEALVRLTSWDPASLAEGEATGAPLGDVRAVVV
                     RRDLPLPVLEAYIATEFARYAWETVLDAVRRAGGSPVGWRALRAEGWS"
     gene            complement(5225..>5959)
                     /locus_tag="VIH70_04125"
     CDS             complement(5225..>5959)
                     /locus_tag="VIH70_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY8115582.1"
                     /translation="ADWGLDVRVLNLTGAYAAVNLAGPASRDVMRSLTDADVSGEAVP
                     YLSATTMDVAGVPALVLRIGFVGELGYEIHVPSMYGEHVWDRVMAAGEPQGIRPFGLE
                     AQRILRLEKQHILVGQDTDAESDPFEVGLGWTVKADKDDFLGKRGLEGLAEEAPGERL
                     VGFACSGTWVPPEGASVVHDGVWVGRVTSARRSAAAGAIVGLAWVPAGWASDGTPFEI
                     QFGGRRADATVAMRPFYDPDGARLRS"
BASE COUNT          881 a         2160 c         2032 g          886 t
ORIGIN      
        1 tcgcaggaga accggcacca ccacgagcag tgcgacgagc gccgcgggga tagtcacgtc
       61 ctcggtcggc tcggtgtccg tgctcggcat cggggcagtg atcgtgaaat cagcgaccca
      121 aaggagcgtg tttccgaacg agaactcggc gcacggctcg caacggagcc tgacttcata
      181 gcgagccgcc ggcagcgccg ggacgacgaa ggagccgacc ccgttgagtc ccgaatcgac
      241 cgtgatctgc ccgacctcca ggggcgtctc ttcggtcgcc gacccgggta aacctccgcg
      301 gagatagagc gacatggcgt cgccgggtcg agcgatggcc atggctccct ggccggccgt
      361 cctcacccga atggtttcgc cgggcgcccc actggtcggg tcaagcgtca cccagagcgc
      421 cgcggccgca tgtcccgacg acacgacgaa cgcgaggacc acgaggacga acacccgtgc
      481 gcacagcgcc gcgtgcggcc gcctcgtcat cgcgcagtgg cgacctccgg gagcttctcg
      541 atgcggatag cggtggcctt gaattcggcc gtgccggact tcgggtcagt ggcgtcgatc
      601 gtcagcaggt tcgtcgccac gtcgtcctgg aagtgcagcg tcatgaacgt caggcccgga
      661 cgcagccccg ggtcgatccg aatcggcact tccacctggc cgcgccgtga gaccgcgcac
      721 acccgttcgc cctcggtcag cccgaaccgt tccgcgtctt ccggcgagat gtcaagggtc
      781 tcgccgcgcc gcatcggcga tgtatagctg gccgtctgca cgccggtgtt gtacgagtcg
      841 aggcgccgac cggtggtgag cctgatcggg tagtccgcgt cgagcttgtc gaccggcgga
      901 tcgtggtcga caggcacgaa cggcgcgcgg tttcccggca ccgggtcctc ccacagccgg
      961 ctgtgcagga acagctcgcc gggatggttc tcgtcgtagc acggccactg gatgccgccg
     1021 agctcctcga gccgcatgta cgtcatcccg gcgtgcactg gtgagagcga gcggacctcg
     1081 tcccacgtct tctccgccga agccgagccc cactccgagc ccattcggcg tgccaggtcg
     1141 taaatgatct caaggtcgtc gcgcgcctcg cccggcggat caagcgcctt gcgcacgcgc
     1201 tgcacgcgac gttccgagtt ggtaacggtg ccttcgcatt cggcccacgc cgcggcggcc
     1261 gggagaacga cgtcggcgag ttcggccgtc gccgtcagga acaggtcctg cacgaccatg
     1321 aagtcgcgcg tcgacagcag gtgcgcggcg cgccgttgat ccgcctcgga ctggagcggg
     1381 ttctcgccga tgacgtagag cgcctgcagc tcgccacgct ccatcgcgtc gaacatcccg
     1441 gacaggtgcc agccgcgctg cggtggaatc gtcacgcccc aggccttgtc gaacttcgcg
     1501 cgcagctcgt cgttctcgac gtgttggaag ccgggcagcc gatccggcag cgcgcccatg
     1561 tccccgccgc cctgcacgtt gttctggcca cggagcgggt tgacgccact cccgtatcgg
     1621 ccgacatgcc cggtcaacag cgccaggttg atcagcgcga ggacgttgtc gaccgcgttg
     1681 tggtgctcgg tgatgccgag cgtccagcag atcatcgccc ggttggcgcc ggcataggcg
     1741 tgtgccaggt cgcgaatcgc gtcggcggga acaccggttt cgcgttcggc gtattcgagc
     1801 gtgtacgact cgaccttgtc gcggtaggcc tcgaaatctg acgtcgcccg gccgatgaat
     1861 tccttgtggt gcagccccgc gtggatgatc tcgcgggcga tcgcgttggc cagcgcgatg
     1921 tcggacccga cgtcgagccc cagccaggta tcggcccatt cggcgctcga cgtgcgccgc
     1981 gggtcgaccg catacaggcg cgcgccgttg cgtacgcccc gcaacaggtg gtggaagaag
     2041 atcgggtgcg tctcgcgggc gttcgagccc cacaggacga tgacatccgt ctcttcgatt
     2101 tcgcggtatg aacttgtgcc gccaccggca ccgaacacgg tcgccagacc ggcgacgcta
     2161 ggggcgtgtc aggtgcggtt gcagctgtcg atgttgttgc ttcgaaggac cgcccgcatg
     2221 aacttctgtg cgacgaagtt catctcgttg gtggccttcg agcagctgaa caggccgacc
     2281 ccgtcaccgg cgttccgctc ggtcgcctcc cgcagacccg cggcggcccg atcgagggcc
     2341 tcgtcccagg tcgccgtccg caggccgccg ttctcgcgga ccatcggctc ggtgagccgt
     2401 gtgagcccgt tcttcatcgg ggcgcgctcc tttcgctcgg ctctagccct gattctaagc
     2461 cgcctcccgg cgctcggccc gctcaggtgg ggacgcgcgc ggcgtggcta tagacgttcg
     2521 cgcgcccgtc gcggacgaac cccaccaggg tcatcccgaa ccgctcggcg gcctcgacgg
     2581 cgagcgacga gggcgcgctg accgcgctga cgatcgggac cccggccacg gccgccttct
     2641 ggacgatctc gaacgacgcc cgcccgctca cctgcaggat cgtcccgtcc gccgggatct
     2701 ggctggccag gtaccgctcg ccgacgacct tgtcgacggc gttgtggcgc ccgacgtcct
     2761 cgcggaccgc cagcgccgac cccgacgcgt cgaacagccc cgcggcgtgg agacccccgg
     2821 tgcgctcgaa caccgcctgc gcctcgcgca gccgttcggg cagcgagatc agcaccccga
     2881 gctcgacctc gggccccgga ccgacgggct cgcatcgcac gtcgatgtcc tcaagcgccg
     2941 ccttcccgca gacgccgcac gaggagttgg cgtagaagtt ccggcggagc cgttcggcct
     3001 cgaagggacg ggcgagcgtg accgacacca cgttgtagcg ctggtcctcg ccgggcaggt
     3061 tgtcgcagta ggccacgcgc tggacgtcac cgggcgcgat cagcccctcg gtgaacaaga
     3121 acccgacggc gagctcgaag tcccccccgg gcgtccgcat cgtcaccgcc acctgcagcg
     3181 ggtcctgacc cggtccctgc acgcggatct cgagcggctc ctcggtggcc aggctgtcgg
     3241 tgcgttcgga gcggatcccg tcgtgcacgg ccaccacccg cgcccgggtg gagctgtggc
     3301 ggatcggttc ggtgacgctc accgctccag tatccgaccg cgggcggcga gggtccatct
     3361 ccgcggtgac cagggcggcg aggctcagtg cgagacggcg gcggcggcga tctccgagac
     3421 gagctcgcgc ttccagaacc ggttgagcga gaacggggcg atcaggtcgg gcaccgtgcc
     3481 ggtgtggatc aggtcggcca tcgtgacgcc cacgatcggg gtggccttga agccgtaggt
     3541 cccccaccct ccgtcgatca ggaacccctc gacctcggtc gtccccatga tgggcgagta
     3601 gtcgggcgag acgtcgcaca ggccgcccca cgcgcgcatc accttcacgc gcgacaggaa
     3661 cgggaggagc tcgagcgcgt gctcggccgt gacctcgagg aagttcagcg tcgagtcctg
     3721 ggcgtacgac gagtacggct cgatctccga gccgatcagc acctcgccac gatcggtctg
     3781 cgagacgtag acgtgcagcg tggccgagac caggatcgcg tggaggaacg gcttgagcgg
     3841 ctcggtcacg aaggcctgca gggggtgcgt ggtgatcggg accctgatcc cggccatccg
     3901 cgcgatctcg gaggtccagc cggccgttgc cgagacgacc accggtgccg agacgtcgcc
     3961 ggcgctcgtc gagacgcccg tgaccttccc gccgtcgacc ctcacacccg tcacctcgac
     4021 gccctcgtgg atctcgacgc ccatccggtc cgcctgccgc gcgtaggccc acaccaccgc
     4081 atcgtggcgg atcaccccac cgggcgggtg gtacagcgcc gccatgatcg ggaaggccgg
     4141 gcgatcggac aggtcgagct gcgggcagag cctgcggatc tcgtcggggt agatgacgcg
     4201 ggagtcgacg cctagcagcc ggttcacctc ggcccgctcg tgcgcgacgt tgaccgcgcg
     4261 ttcggcgtgc gccagcgtga gatggccctg ctgcgagaac agcaggttgt agtcgagttc
     4321 ctgcgcgaga tgctcgtaga gcctgagcgc ctgcttgtag aaggcgaccc cctcgggcgt
     4381 tcggtagttg gcgcggatga tcgtggtgtt gcggcccgaa ccaccggcgc cgatgtagct
     4441 cttctccagg accgcgaccc tcgtgatgcc gcgcttggcg agctcgtagg cgatcgcgag
     4501 cccgtgagcc ccgcctccga cgatcgcgac gtcgtacgcg gacttcaggt cgtggcggcg
     4561 ccacatcgtc cacggccgga agtagcggat cgggtgcatc gctacgacca cccctcggca
     4621 cggagcgcgc gccagccgac cggcgatcca cccgcccgcc gaaccgcgtc cagcaccgtc
     4681 tcccacgcgt acctcgcgaa ctcggtcgcg atgtacgcct ccagcaccgg cagcggaagg
     4741 tcgcgccgga ccacgacggc tcggacgtcg cccagcggcg cgccggtcgc ctcgccctcc
     4801 gcgagcgacg ccggatccca cgaggtcagc cggacgagcg cctcgggcag gtcgggccca
     4861 gcgagcgcga accccacgaa caggtgcgtc gcgtcggtca ccatcgctcc gggccccgcc
     4921 gccgaggcga gcttgggcag aagcacctcc tctcccccgg gttcggaaag cagcagggcc
     4981 cagtcgtcgg cgaggcgggc gacgaggacg tcgcccgcgc ccgacagggc gccgtccagc
     5041 gacccccgga cgtcgatctt gccgcgcgct gtcacgtcgc cgagtccgac gccttcgcgc
     5101 agcgtcgcgc gttccgcggc ctcgtccccg tagtgcgcga cgagccgcca ccccgcctcc
     5161 cggtcctgca cggcgccgag gtcggcgtgc gagcggtcga ggggcgaacg ccggatcggc
     5221 tcgttcacga ccggagcctc gcgccgtccg ggtcgtagaa cggccgcatc gccacggtcg
     5281 cgtcggcgcg ccgaccgccg aactggatct cgaacggggt cccgtcgctc gcccacccgg
     5341 cggggaccca ggcgaggccg acgatcgcgc cggccgcggc gctcctgcgg gccgaggtga
     5401 cccgacccac ccacacgccg tcgtggacca cgctcgcacc ctcgggcggc acccacgtcc
     5461 ccgagcaggc gaacccgacg aggcgttcgc cgggtgcctc ctcggccaga ccctcgagcc
     5521 cgcgcttgcc aaggaagtcg tccttgtccg ccttgaccgt ccagccgagg ccgacctcga
     5581 acggatcgga ctcggcatcc gtgtcctgcc cgacgaggat gtgctgcttt tccagccgga
     5641 ggatccgctg ggcctccagg ccgaacggac ggatcccctg cggctcgccg gccgccatca
     5701 ccctgtccca gacgtgctcc ccgtacatcg acgggacgtg gatctcgtag cccagttcgc
     5761 cgacgaaccc gatccgcagc acgagggccg ggacgccggc gacgtccatc gtcgtcgccg
     5821 acaggtacgg gacggcctcg ccggagacgt cggcgtcggt gagcgaacgc atcacgtcgc
     5881 gcgacgcggg accggcgagg ttcaccgccg cgtaggcgcc cgtgaggttc agcacgcgga
     5941 cgtccagacc ccagtcggc
//
LOCUS       DATBRK010000137        12094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2042169, whole genome shotgun sequence.
ACCESSION   DATBRK010000137 DATBRK010000000
VERSION     DATBRK010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12094
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042169"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..471
                     /locus_tag="VIH70_04130"
     tRNA            411..471
                     /locus_tag="VIH70_04130"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:432..434,aa:Lys,seq:ctt)
     gene            661..1107
                     /locus_tag="VIH70_04135"
     CDS             661..1107
                     /locus_tag="VIH70_04135"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8115583.1"
                     /translation="MKTFEAQTKIRAPAQTVFDYVSDFTKHGQWAGHGLQVTKDSDGP
                     VAIGSTFSTVAKQFGTQREHSTVTELEPGRVFGWDSKGALGTVHHRFTVAAADGGTTL
                     TKSAEFLDRSFLAKVTSWKVSRDVPAGLNGDVEKIRAHLESPSSSS"
     gene            complement(1232..1774)
                     /locus_tag="VIH70_04140"
     CDS             complement(1232..1774)
                     /locus_tag="VIH70_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEY8115584.1"
                     /translation="MSELEDFRAEKDEFFRHHPQSPLTHEQRAAFEGLAYYPENDALV
                     IRETLEKDRVDLDEVIVIQTTTGGTQSYRRAGIVRFPVGGAPAQLTLFTSSDAHELFL
                     PFRDATSGKETYGAGRYLEVEPPGLDELVVVDLNYAYNPYCAYNPDWSCPIPPGENWL
                     AVPLRAGEKSFPGAYEVAIA"
     gene            1992..2516
                     /locus_tag="VIH70_04145"
     CDS             1992..2516
                     /locus_tag="VIH70_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY8115585.1"
                     /translation="MVSRRRYVVLLRAISNVSMKPFRDSIEEMGFTDVESYAASGNLL
                     FDARTTDARALERRIGRRFGTDAFVRTPTEMARVVAQDPLGAIVMFLERPPTTSRKRA
                     FLDLDVREPRPVLRGRTLYLSYPLLLRGRRTHLDVEDALAVRGTFRTSRVVATLLARI
                     SARTRDDQARRTRR"
     gene            2659..2964
                     /locus_tag="VIH70_04150"
     CDS             2659..2964
                     /locus_tag="VIH70_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8115586.1"
                     /translation="MREPLIPMDDIPPEGTVTADLLGREVLVMMLNGRPRAYLNVCMH
                     HGGPLTLDGDTFTCQWHGSTFDARTGKALSGPVRPDARLIMLPTRVEDGVLTYVYEE"
     gene            3050..4018
                     /locus_tag="VIH70_04155"
     CDS             3050..4018
                     /locus_tag="VIH70_04155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8115587.1"
                     /translation="MEYVNLGKTGLRVSRIVLGMMSFGKDPGRAWALDEDAAEPIVRR
                     AVEGGTTFFDTADAYNGGQSEVVTGHLLPKLFGMREEYVVATKVFFDTMPGENGRGLS
                     RKHVLASIDASLDRLGLDYVDLYQIHRWDPLTPIEETMEALHDVVKAGKARYLGASSM
                     DAWQFAKAQRVAPTPFVSMQNHYNLVYREEEREMIPQCVDQGVALLPWSPLARGLLAG
                     NRTREGERLTTRSRTDEFGESLYTPEVDFAVVDRVTEVASARGVSSAQVALAWLLHKP
                     AVTAPIVGATRVEHLDDAIRAVGLALTEDEIGRLEDPYVPHAISGH"
     gene            complement(4042..5277)
                     /locus_tag="VIH70_04160"
     CDS             complement(4042..5277)
                     /locus_tag="VIH70_04160"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8115588.1"
                     /translation="MSVSREQLVQRAGVNDDYVDSLHELGALRGRDDAYEERDVHWAA
                     LLYMWKQAGLSAPAILAAVDAGTLSLDFLDSPAWELPEPLPITYSELASKHQVPLHLL
                     GGIQEAMGFASPDPGDRVRPDDIVLAELARVVLDIGASDEGVRRLFRVYADNIRRLAM
                     AEADLYLEELEKTWAGAGVHESDLMLRGAEVGRRMTGLVEGTIRAIYDRHRQHIWTEY
                     GVRRAEMALERAGLLERAESTPAICFVDMTGFTRLTEERGDEEAARLAISLAALVNDV
                     SRTYGGRAIRWLGDGGLFYFEDASAAFAAALEMSERAPAAGLPPTHIGVQAGPVVFRD
                     GDVYGRTVNIASRIADQAEAGEVLTSQETVTSVGDVQASFERARSVELRGVTSPVALY
                     RVLRRAERNSTLDRSDAPP"
     gene            complement(5378..5873)
                     /locus_tag="VIH70_04165"
                     /pseudo
     CDS             complement(5378..5873)
                     /locus_tag="VIH70_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012296234.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
     gene            complement(5897..6118)
                     /locus_tag="VIH70_04170"
     CDS             complement(5897..6118)
                     /locus_tag="VIH70_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115589.1"
                     /translation="MTSRGSSRIRIALFGLLTALVLAFSACASRGRPETETTSRTSKV
                     AELVGVSDLRERFNEDSGKVRLILLISPT"
     gene            complement(6248..6679)
                     /locus_tag="VIH70_04175"
     CDS             complement(6248..6679)
                     /locus_tag="VIH70_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEY8115590.1"
                     /translation="MHGVATGHGDVDHVVIGPTGVFALETKAWRGSFYQKRGQLFSNG
                     KPAEHVLRQARGAAGQVRQHLLEAGIDEWVEAIVVASRASVSRSPLRFPNAYVVSING
                     VVDLITGRRRWIRGSEVLAAVASLVEPGGDDGSVDRGGSEP"
     gene            complement(6683..6943)
                     /locus_tag="VIH70_04180"
     CDS             complement(6683..6943)
                     /locus_tag="VIH70_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115591.1"
                     /translation="MPEPAEFLRSQLRLRIAVFAATVAVAGVLALMGAWSWPTILPAI
                     GLLFVLERLAGKGRVLDPENLRSGILGEEAVAEALAALPSSY"
     gene            7137..8513
                     /locus_tag="VIH70_04185"
     CDS             7137..8513
                     /locus_tag="VIH70_04185"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8115592.1"
                     /translation="MRPVEVPETHTPRPHSTTPSSPNPSPSRVGWTVRATAWLGRIGA
                     DPRDDEELRQRKSLLVLIAVLILPVSLVWGSLYLAFGSPVGIVPYIYFAVSLGSLVIF
                     ARTRNFQFLLVTQLLDILITTTVGMMLVGGFLPSGGVGLWGILAPLGALVFLEVRQAV
                     RWFAAFVLIFLLLGIAGEVLFSDADLPVGFTSTMLVLNIVGAASVAFTLLAVFAQQRN
                     AALTALRAEQDRSEALLLNILPRAIAERLKADSETIADHFDATTILFADVVDFTPLAQ
                     GLPPVEVVGILDHLFSHFDSLVERHGLDKVKTIGDCYMAAAGVPDPHPDHARRAALLA
                     LDMQDVVATSAVAGRPGLELRIGINSGPVVAGVIGTKRFLYDLWGDSVNTASRMESQG
                     TPGEIQITRATYELLKDEFECRRRGTVQVKGKGQMETWYLVGPRSDDRRTESGGESAA
                     GGHHQDRA"
     gene            8802..9668
                     /locus_tag="VIH70_04190"
     CDS             8802..9668
                     /locus_tag="VIH70_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115593.1"
                     /translation="MRGTPIDLASLTGRIVLSSEDDVFTANADGTGVRRVTRKRGPEF
                     DPAWSPDGERIVYRDSRRGINQDDEIYVVHADGTHARNLTRDRGNDWGPDWSPDGRAI
                     VFNSDRDGLPMGGFLMDPDGSHLRRIPTDAYVEYPAWSPDGTRIAFMGGTSASEHDIW
                     VVDRDGSNLTRLTDSPGPDGWPAWSPDGTRVAFSSVRDDCVYSDAPDCRSTGDIGPHH
                     DVWIVNADGTGLSRVTPEFGQFMTWSPDGRYLLVSGYDLYVIRPDGTGRTTLHLEGLR
                     GGLLPDWIDPDP"
     gene            complement(9677..10030)
                     /locus_tag="VIH70_04195"
     CDS             complement(9677..10030)
                     /locus_tag="VIH70_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8115594.1"
                     /translation="MSRSTKQLPRLDAEACGRADGMLMRAFDLLGKRWTGVVLGTLSG
                     GPTGFRALARAVEGISDSVLSDRLGELTGAGLVTRTVAEGPPLSVAYALTDAGRALLP
                     ALAQISRWAEDHLPP"
     gene            10104..10922
                     /locus_tag="VIH70_04200"
     CDS             10104..10922
                     /locus_tag="VIH70_04200"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEY8115595.1"
                     /translation="MRQPVQETSRDAILVEDAHVRPMMGFEILEPLPARSVPYERVDP
                     FILVHEARFRLSELQDVDTKHPHRGFDNLWYILQGSASTGHSTGPGGTVERARLPEGA
                     LLALRTGRGAWHAEAIGAEQREEGLGDTEWRSVLFWVNLARKDKQAEPSARVLLPEQM
                     TVRKEGDATVRVLAGEGSPVELGTPATVLDVEFPDGGEVTTPVPPDYQGFAYVLDGEA
                     VFGANRRPVRPPQLVLLGPGEDLTVTDAVPGARFLLMAGRPYGEIPVFNGPYVD"
     gene            11028..>12094
                     /locus_tag="VIH70_04205"
     CDS             11028..>12094
                     /locus_tag="VIH70_04205"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY8115596.1"
                     /translation="MSARAVRRTAFAASVVTVLVLAWVLVRTLDPAPSGGGEPRARGG
                     GSEVEDELSEQTEERLEALEEANEQGVRWQVRTKAAAESAAPAPGWAGERVADPVADD
                     WEPAIAADPTAPFVYLMTTHFADKPCPGNCPVPHISLHVSSDGGRTWAPQRPLCACKG
                     SWQYDPVVEVVPNTGAVYAAYLNGFNVVFTSSVDHGATWSAPVKTYGNVSWNDKPALA
                     TSDDGKDVYISWNGPTGGDPWMAQSHDSGQTWTQAKLVDSPRYFFAFDADVLHDGTVV
                     FAQSDIDYSGPGGAPVGVVNHHVFVSRDTGATWEDHVVDTVAVGEPCIAEGCGPDFYI
                     GHDAISADDAGGLVVLYDGAAT"
BASE COUNT         1930 a         4120 c         4190 g         1854 t
ORIGIN      
        1 atccatgagc acggccactt gggtcgggtt ctgcgggtcg acgaacgtcc gaatgttggt
       61 gacgcccagc gggccgagga cctcttcgcg cttcggcgag gcaagccagt ggtccttgtc
      121 ctccttcacg tcgtgatgga catcacggtg agcacggtgc cccctcgtcg tcgtgggccc
      181 agcgaaagcg ccggacccgg cgagcgcgat cgtacgcgtt gtgcctgatt cctggcacgc
      241 tacgcggagt cagcggaatg ttgctccttc ggcgccatgt ggcatcgaaa tgacaacgcc
      301 gcgcggcgtg ttgtccccgt ctcatccccg tgaaggaacg ggaatcgacg ggaaacagtg
      361 ggtagcactg cgacgcaaac tcgcagttca gacgcggtgc agctccccga cctgcagcgt
      421 cagaccggac tcttaatccg caggttcggg gttcgagtcc ccgggggccc acaagtccag
      481 cggtaccgct cgagatcgtt ctcgtcggtc atcgtcagaa gaggatgcct tgcgtgcgga
      541 cgtgctcggc gcgccgtgcc gtcgacgcga gctgagcgac gcacctgatc cgctcgctcg
      601 tcggacgcgc ggatgaccgc tgcgataggc tcccagcgca tctcgacacg agggggccgc
      661 atgaagacgt tcgaagccca gacgaagatc agggcgccgg cccagacggt cttcgactac
      721 gtgagcgact tcacgaagca cggccaatgg gccgggcatg gcctgcaggt cacgaaggac
      781 tcggacggcc cggtcgcgat cgggtcgacc ttctcgaccg tcgcgaagca gttcggcacg
      841 caacgcgagc acagcacggt caccgagctc gagccgggca gggtcttcgg ctgggactcg
      901 aagggcgcgc tcggcaccgt gcaccatcgg ttcacggtgg ccgcggccga cggcgggacg
      961 accctcacga agagcgcgga gttcctggac cgctcgttcc tggcgaaggt gacgagctgg
     1021 aaggtctcgc gagacgtacc ggcggggctc aatggagatg tcgagaagat cagggcgcat
     1081 ctcgagtcgc cgtcgagctc gagctgaggg gcgcgcaaga ccccgacgaa acgccctgca
     1141 cagggaagcg cttgatcctc caccactcgg catcgcgagc gtccaggcac tccgcctgga
     1201 ctggcgcacg tatcggggac cgcggcgcag ctcaggcgat ggcgacctcg tacgcacccg
     1261 ggaagctctt ctcgccggcg cggagcggga ccgcgagcca attctctccc ggtgggatcg
     1321 gacagctcca gtcggggttg taggcgcagt aggggttgta cgcatagttg aggtccacga
     1381 cgaccagctc gtcgagcccc gggggctcca cttcgaggta gcgcccggcc ccgtacgtct
     1441 ccttcccgct cgtcgcgtcc cggaacggaa ggaacagctc gtgcgcgtcc gaggacgtga
     1501 acagcgtgag ctgtgccgga gcgccgccga ccgggaaccg gacgatgccg gctcgtcggt
     1561 aggactgcgt tcccccggtg gtcgtctgga tcacgatcac ctcgtcgaga tcgactcggt
     1621 ccttctcgag cgtctcgcgg atcaccagag cgtcgttctc cgggtagtag gcgagcccct
     1681 cgaacgccgc gcgctgctcg tgcgtgagcg gcgactgcgg gtggtgacga aagaactcgt
     1741 ccttctccgc ccggaagtcc tccagctcgc tcatcaaatg tgaccaaccg cttgggggcc
     1801 gttgcccatt cccgcgttcg gcccccggcc tcacgccccg tccgtcaaca gatcggatgc
     1861 ggcctccgac gtattcacct tcctgtgatg gatccgcatc agccgccacg tagggccgag
     1921 ccgagagacc tgccgtcgcc ggcgtgcgag gatgcggctc gagaactcga gcgccatgac
     1981 gagggagcag gatggtcagc cgacgtcgat acgtggtgct gcttcgcgcc atcagcaacg
     2041 tatccatgaa gcccttccgg gattccattg aggagatggg cttcaccgac gtcgagagct
     2101 acgcggcgtc gggcaacctt ctgttcgacg cgcggacgac cgatgccaga gcgctcgagc
     2161 gaaggatcgg ccggcgcttt ggcaccgacg cgttcgttcg cacccctacc gagatggcgc
     2221 gagtggtcgc tcaggatccg ttgggtgcga tcgtcatgtt tctcgaacgc ccgccgacga
     2281 cgtccaggaa gcgcgcgttc ctggacctcg acgttcgcga gccgcggccg gtgctccggg
     2341 gcagaaccct ctatctctcg tacccgctgc tcctgcgcgg acgacggacg catctggatg
     2401 tcgaggacgc gctcgcggtc agggggacgt tccgcacgtc gcgcgtggtg gcgaccctct
     2461 tggctcgcat atcggctcga acgcgcgacg accaggctcg gcgcacaagg cgatagtcgg
     2521 ggtgcgcggc cccattcctt tcccgatctc ccttgcgggc accgacgccg gtgcgatagg
     2581 caggagtgga gacggatcgc atcccgacgg tatcggggtc gttccgcccg gtcggaacct
     2641 cgagcccgga ggcgacgcat gagagagccc ttgatcccca tggacgacat cccgcccgag
     2701 ggaacggtga ccgccgacct cctcggccgg gaggtgctcg tcatgatgct gaacgggagg
     2761 ccccgggcat acctgaacgt ctgcatgcac cacggcggcc cgttgacgct ggacggcgac
     2821 acgttcacct gccagtggca cggctcgacg ttcgacgccc gaacgggcaa ggctctgtcg
     2881 ggaccggtcc gccccgacgc gcggctgatc atgctcccga cccgcgtcga ggacggcgtc
     2941 ctgacctacg tgtacgagga gtaaccgggg ggccgaggag aaggcccgcg ccgagggtgg
     3001 ggacacgccg gcgattcaca cttgtcggcg gagggtagcg tagagaccga tggagtacgt
     3061 gaacctcggc aagaccggcc tccgcgtctc gcgcatcgtg ctcgggatga tgagtttcgg
     3121 taaggacccc ggccgcgcgt gggccctgga cgaggacgcc gccgaaccca tcgtgcgccg
     3181 cgcggtcgag ggcgggacca ccttcttcga cacggcggac gcgtacaacg gcggccagag
     3241 cgaggtggtc accgggcacc tgctcccgaa gctgttcggc atgcgcgagg agtacgtggt
     3301 cgcgacgaag gtgttcttcg acacgatgcc gggcgagaac ggccgcggcc tgtcgcgcaa
     3361 gcacgtgctc gcttcgatcg acgcgtcgct ggatcggctc gggctcgact acgtcgacct
     3421 gtaccagatc caccggtggg acccgctcac cccgatcgag gagacgatgg aggcgctcca
     3481 cgacgtggtc aaggctggca aggcacgcta cctcggggcg agcagcatgg acgcgtggca
     3541 gttcgcgaag gcgcagcgcg tcgcgccgac gccgttcgtc tcgatgcaga accactacaa
     3601 cctggtgtac cgggaggagg agcgcgagat gatcccccag tgcgtcgacc agggggtcgc
     3661 cctgctgccc tggagcccgc tcgcacgcgg cctgctggcc gggaatcgaa cgcgcgaggg
     3721 cgagcggctc acgacgcgct cgaggaccga cgagttcggc gagagcctct acacgcccga
     3781 ggtcgatttc gcggtcgtcg accgcgtgac cgaggtggcg tcggcgcgcg gggtctcgag
     3841 cgcccaggtt gcgctcgcat ggctgctgca caagcccgcg gtgacggcgc cgatcgtcgg
     3901 ggcgacgagg gtcgaacacc tcgacgacgc catccgtgcc gtgggactgg cgctgaccga
     3961 ggacgagatc ggcaggctcg aggacccgta cgtcccgcac gcgatctcgg gtcactagga
     4021 gcgggcgagt tcgagcccgg cctacggcgg ggcgtccgaa cgatcgaggg tcgagttacg
     4081 ctcggcccgc cgaagcactc ggtagagggc cacgggactc gtgactcccc ggagctcgac
     4141 cgagcgcgcc cgctcgaacg acgcctgcac gtcacccacg ctcgtgacgg tttcctggct
     4201 ggtgaggacc tcaccggcct cggcctgatc cgcgatgcgc gaggcgatgt tgaccgtgcg
     4261 gccgtacacg tcgccgtctc ggaagaccac cgggcccgcc tggaccccga tgtgcgtggg
     4321 aggcagcccg gcggcggggg cgcgctcgct catctccaga gccgccgcga acgcggcgct
     4381 cgcgtcctcg aagtagaaca ggccgccgtc gcccagccat cggatcgccc ggccgccgta
     4441 ggtgcgtgag acgtcgttca cgagcgccgc aagcgagatc gccagccggg cggcctcctc
     4501 gtcgccccgt tcctcggtga gccgcgtgaa cccggtcatg tcgacgaagc agatcgcggg
     4561 ggtcgactcg gcacgttcca ggagcccggc tcgttcgagc gccatctcgg cacgtcggac
     4621 gccgtactcg gtccagatgt gctgccggtg tcggtcgtag atcgcgcgga tcgtgccctc
     4681 gacgagcccg gtcatccggc ggccgacctc cgcgcctcgc agcatcaggt ccgactcgtg
     4741 gactccggcg ccggcccacg tcttctccag ctcctccagg tagaggtccg cctcggccat
     4801 cgcgaggcgc cggatgttgt cggcgtacac ccggaagagg cgccgaacgc cctcgtccga
     4861 agcgccgatg tcgagcacga cgcgggcgag ctccgccagg acgatgtcgt ccggccggac
     4921 ccggtcgccg ggatcgggcg acgcgaaccc catcgcctct tgaatgcccc caaggagatg
     4981 cagcgggacc tggtgcttcg acgcgagctc gctgtacgtg atcgggagcg gctcgggcag
     5041 ctcccacgcc ggcgagtcca agaagtccaa ggacagcgtg ccggcgtcga cggcggcgag
     5101 gatcgccggc gcggacagac ccgcctgctt ccacatatag agcagcgcgg cccagtggac
     5161 gtcgcgttcc tcgtacgcgt cgtcgcggcc gcgcaacgcc ccgagctcgt ggaggctgtc
     5221 gacgtagtcg tcgttcacgc ccgcccgctg gaccagctgc tcgcgcgaga ccgacatgag
     5281 agtagttcat cacgtctcgt cggcctacgg atcgcgaact catcggttcc aaccgcatcc
     5341 gccctcggcc gattccgacg tgcctcggat cagaggctca ggcggcgcag caggagagca
     5401 tctgcacgtc ggtcgagaag ctctgtcgtg agcctgatcc cctccgccat ggtgagatac
     5461 ggagcccacg tgtcggccag ctcgtcgacc gtgaggccga acttcactgc gtacacgccg
     5521 gcgaggatcg catcgcccgc gttggcggcc agtgcgtgga caccgagcac cctgccggtc
     5581 tccgcatggg ccacgatctt gaacagccct cgcgtatgga ggtcgacccg cgctctcggg
     5641 acgtggcgga gctccacgac gcggcactcg caccggtagc cggcggcgac cgcctgctcg
     5701 tcggtgagtc cgacggccgc gatgtttggt gacgtgaacg tcacacgcgg cagggccgtg
     5761 tagtccatcg tccggaccgc ggcgccgacg gcgttgtcgg cgacgagcgt tccctgtggt
     5821 gcggcgatgt agacgaactg gagagcatcg gtcacgtcgc cggcggcgta gacgttgcgc
     5881 cagcgaggca cgtcgttcag gtaggcgaga tcaacaggat cagtcggacc ttccccgagt
     5941 cctcgttgaa ccgctcgcga aggtcggaca cgccgacgag ttccgcgacc ttggacgtcc
     6001 gtgaggtggt ctcggtctca gggcgtccgc gcgacgcgca ggcagagaac gcgaggacga
     6061 gcgcggtcag gaggccgaac agcgcgatcc ggatccggct cgacccacgg gaggtcatca
     6121 gaaggtgcac ccagaccacc tccgtggttc atggtaggcc ctatgaggct cgggccccga
     6181 cggcgcgcgg tcacatgcac cgatgcgtcg aacgggtccc gaaccgggtc gaggcgtcgt
     6241 ctgcccgtca gggctccgag ccgccacgat cgaccgagcc gtcgtcgcct cccggctcca
     6301 ccaggctcgc caccgccgcc aggacttccg agccgcggat ccagcgacgc cggcccgtga
     6361 tgaggtccac gacgccgttg atcgaaacga cgtaggcgtt cgggaagcgc aggggggagc
     6421 gtgagacgga ggctcgcgag gcgaccacaa tcgcctcgac ccactcgtcg atgccggcct
     6481 cgaggagatg ctggcggacc tgccccgcgg cgccccgcgc ctgccgcaac acgtgctcgg
     6541 ccggcttccc gttgctgaag agctgtcctc gtttttgata gaacgacccc cgccaggcct
     6601 tggtctcgag cgcgaacacg ccggtggggc cgatgacgac gtggtcgacg tccccgtgcc
     6661 ccgtcgcgac gccgtgcagg acctagtacg aggacggcag cgcggccagg gcctccgcga
     6721 ccgcctcttc acccaggatc ccgcttcgga ggttctcggg atcgaggacc cggcccttcc
     6781 ccgccaggcg ttccaggacg aacaggagcc cgatcgccgg gaggatcgtc ggccacgacc
     6841 acgcgcccat cagggcgagc acgcccgcga ccgcgacggt cgccgcgaag acggcgatcc
     6901 gcaggcgcag ctgcgacctc aggaactcgg cgggttcagg cacgtatgtc tcatcggccg
     6961 gtcgggcccg ccggtgaaag cgcaggcacg gctttgcctc caaatcggca tccaacggcc
     7021 ggccggctcg cccgcggaga ggcgactcga gctcctcggc gtgagcggcg ccagggatgc
     7081 tcgggggtgc ctcgtaggca cggagcgttt gccgcgtccg tcgggaaggg gtgaagatgc
     7141 gccccgtgga ggttccggag acgcacacac cgagaccgca ttcaacgacc ccctcctctc
     7201 cgaacccatc cccttcgagg gtcggctgga ccgtcagagc taccgcgtgg ctcggtcgga
     7261 tcggcgcgga tccccgggac gacgaggagc tgcgtcagag gaagtcgctc ctcgtcctga
     7321 tcgccgtcct gatcctgccg gtttctctcg tctggggcag cctctacctc gccttcggct
     7381 cgccggtcgg gatcgttccg tacatctact tcgccgtctc gctcggttcg ttggtgatct
     7441 tcgcgcgaac ccgaaacttc caattcctcc tggtcaccca actcctcgac atcctgatca
     7501 cgacgacggt cgggatgatg ctggtcggcg ggttcctccc ctccggaggg gtcgggttgt
     7561 ggggcatcct ggctccgctg ggagcgctcg tcttcctcga ggtgcgtcag gcggtccgct
     7621 ggttcgccgc gttcgtcctg atcttcctgc tcctggggat cgcgggcgag gtgctgttct
     7681 cggacgcgga tctcccggtc gggttcacca gcacgatgct ggtgttgaac atcgttggtg
     7741 ctgcatccgt cgccttcacg ctgctcgcgg tcttcgctca gcagcgcaac gcggcgctga
     7801 ccgccctccg agccgagcag gacaggtccg aggccctcct gttgaacatc ctgccgcgcg
     7861 ccatcgccga gcgactgaag gccgattccg agacgatcgc cgaccacttc gacgcgacca
     7921 ccatcctgtt cgccgacgtc gtggacttca ccccgctcgc gcaaggcctc ccgcccgtcg
     7981 aggtcgtcgg catcctcgat catctcttct cccacttcga ctcgctcgtc gagcggcacg
     8041 ggctcgacaa ggtcaagacg atcggcgatt gctacatggc cgccgccggc gtgccggatc
     8101 cccatcccga tcacgcgcgc agggccgcgc tcctggcgct ggacatgcaa gacgtcgtcg
     8161 cgacctcggc ggtggccggc cgaccgggtc tcgagctgag gatcggcatc aactcggggc
     8221 cggtcgtcgc cggggtgatc ggcacgaaac ggttcctcta cgacctttgg ggcgattccg
     8281 tgaacacggc gagtcggatg gagtcgcagg gaacacccgg tgagatccag atcacccgcg
     8341 ccacgtacga gctgttgaag gacgagttcg agtgcaggcg acggggaacg gtccaggtca
     8401 aggggaaggg ccagatggaa acctggtacc tcgtggggcc ccggtcggac gatcgacgaa
     8461 cggagagcgg cggcgagtcg gcggcggggg gccaccacca agaccgcgcg tagatccctc
     8521 ggttccatgt cgatgcgagc ggctgcaccc gcctccggag gtgcgccggc cgggaacccc
     8581 aggcttcccc agggtgtatc ccacggtgag gagcggagga tctccacatg ggccttgggc
     8641 aacatcggtg ggttgtcgct ctggtcacgc tcctggccgc agcggcggtg tcgtgcacga
     8701 catcgtcgcc ctcggccggc ggtggatcga cgacggagcc ctcggccacg gacacggcgt
     8761 cggagccatc gagatccccg gtcgactctc gcgagcaggt cgtgagaggg acaccgatcg
     8821 acctcgcctc cctcaccggc cgcatcgtgc tgtcgtccga ggatgacgtg ttcacggcca
     8881 acgccgacgg cacgggcgtt cgccgagtca ctcggaaacg aggtcccgag ttcgacccgg
     8941 cctggtcgcc cgacggggag cggatcgtct atcgggactc acggcgcggc atcaaccagg
     9001 acgacgagat ctacgtggtg catgccgacg gcacacacgc gaggaacctc acgagggacc
     9061 gggggaacga ttggggtccg gactggtccc ccgacggccg tgcgatcgtg ttcaactccg
     9121 atcgcgacgg gctgccgatg ggcgggttcc tgatggaccc cgacggatcc catctccgtc
     9181 ggatcccgac ggacgcctac gtcgagtacc cggcctggtc gccggacgga acgcgcatcg
     9241 ccttcatggg cggaacgagc gcgagcgagc acgacatctg ggtcgtggat agggacgggt
     9301 cgaacctgac ccggctcacc gactcacccg gccccgacgg gtggcccgcc tggtcgccgg
     9361 acggcacgcg ggtggcgttc agctcggtac gggacgactg cgtctactcg gatgctccgg
     9421 actgccgatc caccggcgac atcgggccgc atcacgacgt ctggatcgtg aacgcggacg
     9481 ggaccggact gagcagggtc acgcccgagt tcggtcagtt catgacgtgg tccccggatg
     9541 ggaggtacct gctcgtctcc gggtacgacc tgtatgtgat ccgaccggac ggcaccggcc
     9601 gcaccaccct tcacctcgag gggctccggg gcggcctact ccccgactgg atcgaccccg
     9661 acccctaggc cgatccctat ggtggaaggt ggtcttcggc ccagcggctg atctgagcga
     9721 gcgcgggcaa cagggctcgg ccggcatcgg tgagggcgta ggccacggac aggggcggcc
     9781 cctccgcgac ggtgcgggtg acgaggccgg cgccggtgag ctcgccgagc cgatcggaga
     9841 gcaccgagtc gctgatcccc tcgaccgcgc gtgcgagcgc ccggaagccg gtcggcccgc
     9901 cgctcagcgt gcccagcacc actcccgtcc agcgcttgcc gagcaggtcg aaggcgcgca
     9961 tcagcatccc gtccgcgcgt ccgcacgcct cggcgtccag acgcggtagc tgcttggtcg
    10021 atcggctcat gggaataaca ctagcagact cctagttgct agcgggatag aagtgatccc
    10081 ccactcgaag ggagatgagg accatgcggc aacctgtcca ggaaacgtcg cgagacgcga
    10141 tcctcgtgga ggatgcccac gtccggccga tgatgggctt cgagatcctc gagcccctac
    10201 cggcccggag cgtgccctac gaacgggtgg acccgttcat ccttgtccac gaggctcgct
    10261 tccggctctc ggagctccag gacgtcgaca ccaagcatcc ccaccggggg ttcgacaacc
    10321 tctggtacat cctccagggc tcggccagca ccggccacag cactggacca ggaggaacgg
    10381 tcgagcgagc tcgactgccc gagggcgcgc tcctggcgct caggaccgga cgtggcgcat
    10441 ggcacgccga ggcgatcggc gcggagcagc gcgaggaggg gctcggcgac acagagtggc
    10501 gcagcgtgct gttctgggtg aacctcgctc gcaaggacaa gcaggcggag ccgagcgcgc
    10561 gggtgctgct gccggagcag atgaccgttc ggaaggaggg ggacgcgacg gtccgggtgc
    10621 tggccggcga gggttccccg gtggagctgg ggactcctgc cacggtcctc gacgtcgagt
    10681 tccccgatgg cggggaggtc acgacgcccg tcccgcccga ctaccagggt ttcgcctacg
    10741 tgctcgacgg cgaggccgtg ttcggcgcca accgccgacc ggtgaggcct ccgcagctcg
    10801 tcctgctggg tccgggtgag gacctcacgg tgaccgatgc cgttcccgga gcgcggttcc
    10861 tgctcatggc cggacggccg tacggagaga ttcccgtgtt caacggcccc tacgtggact
    10921 agatcgcacg cgcaagcaag gctggagggg gacctcggcc aaggcggtcg cctgcagagg
    10981 tcctgcgcgt cgtgatagga aggagccgac ctgagcggag ggggacgatg tcggctcgag
    11041 ctgttcgacg gaccgcgttc gccgcatcgg tggtcacggt actcgtgctc gcctgggtac
    11101 tcgttcggac cctcgatccg gctccgtccg gtggcggcga gccgcgggcc cggggcggcg
    11161 gctcggaggt cgaggacgaa ctgtccgagc agacggagga gcggctcgag gcgctcgagg
    11221 aagcgaacga gcagggcgtc cggtggcagg tccgaacgaa ggcggccgcg gagtcggccg
    11281 caccggcgcc ggggtgggcc ggcgagcgcg tcgccgatcc ggtcgcggat gattgggagc
    11341 ccgcgatcgc ggcggatccc accgccccgt tcgtgtacct gatgacgacc cacttcgcgg
    11401 acaagccctg tccgggcaac tgcccggtgc ctcacatctc gctccacgtc tcctcggacg
    11461 gggggaggac gtgggcgccg caacgaccac tctgcgcctg caagggctcg tggcagtacg
    11521 accccgtcgt cgaggtggtc ccgaacaccg gcgctgtcta cgccgcctac ctgaacggct
    11581 tcaacgtggt gttcacgagc tcggtcgacc acggcgccac gtggtcggcg cccgtcaaga
    11641 cctacggcaa cgtctcgtgg aacgacaagc cggcgctcgc cacgagcgat gacggcaagg
    11701 atgtgtacat ctcctggaac ggcccgaccg gcggggatcc ctggatggcc cagtcacacg
    11761 actccgggca gacgtggacc caggccaagc tcgtcgactc accccgctac ttcttcgcat
    11821 tcgacgccga cgtgctgcac gacggaaccg tcgtcttcgc ccagagcgac atcgactact
    11881 cgggcccggg cggtgccccc gtcggcgtcg tgaaccacca cgtgttcgtc tcgcgagaca
    11941 ccggtgccac gtgggaggac cacgtcgtcg acacggtggc cgtgggcgag ccgtgcatcg
    12001 ccgagggctg cgggccggac ttctacatcg ggcacgacgc catctcggcc gacgacgccg
    12061 ggggcctcgt cgtgctctac gacggcgccg cgac
//
LOCUS       DATBRK010000138         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2475677, whole genome shotgun sequence.
ACCESSION   DATBRK010000138 DATBRK010000000
VERSION     DATBRK010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2475677"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1306
                     /locus_tag="VIH70_04210"
     CDS             233..1306
                     /locus_tag="VIH70_04210"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEY8115597.1"
                     /translation="MAEAQARGGPARVLSLDLVERDRLDAAASFVVDLLERPASEIRV
                     LEGQLGLDDALVVPGHPDAFELSPVTPVEPGTVWAVDGGSCIVADGRSFQVAAFRAAR
                     IRFEDGQTVDVERPQLDVRAVAPEEMARLARTTLTELCGSEPDRLPELPRPVDALREW
                     AEWGAVGRTVREAAPGDLVLVDGSLHGGPLVPERVVLRVHEEAVARRVSLAGVVKAST
                     LYWGRNAPLVTLLKRRGDREVGPSRWATRISTDPVFGRLYVGDIFVAHLAPTASHAFR
                     VDVARGPAPALQVLGRLAGLADDPAFVGYPYPLARAHQAARVTGYDVVDVRRAFREAL
                     SRRGMAEDDVELLFQDFHEVLNR"
     gene            1303..2937
                     /locus_tag="VIH70_04215"
     CDS             1303..2937
                     /locus_tag="VIH70_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8115598.1"
                     /translation="MTGGAGGSRSERERASGSPPFGGGAGYKGRIFGRNVLESTFRSF
                     YGTDLFLGELLAAEDPERGRRFLFRVIQVTYGSDSSDPSWSERTAGSYMSGDVAGTEY
                     TLRDPEQRLYKVATCQPLGYIDAAGAFRKPKSLPAQFARVTAPTAEDFDFLRERMGDV
                     RVGLLRSGEDTIDLEIGVQGDTLTSHVGVFATTGMGKSNLMKVLAGQILASQGRYACL
                     LFDPHGEYLEGGSGTRKGLRSHPWAPDRLKVYATRAAGAGATSLRLSLAELTQGDLQT
                     AYEWSHAQEDAIYRLIRYFHEEWIVRVGEEQDVASLAEDLGVATSTMQVIQRRAQRIL
                     DMPCISRDPSQPSLSARIVDELMDGFSVLVDTSGLSSMEEILVASVITRTVLDAYANL
                     YLEDRDRFAALPPTLVGLEEAQRVLSRVARSDENVFPRLAREGRKFKVGLCAVSQQPK
                     LIDDELLSQFNTFFVLGLADEKDRNILRGSSKQDISDLGPEIQTLMPGEAIVTNLESP
                     FALPAQIYLYEDYLKSVAGPSPLPPRPSDASKGFAE"
     gene            2938..>3413
                     /locus_tag="VIH70_04220"
     CDS             2938..>3413
                     /locus_tag="VIH70_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8115599.1"
                     /translation="MARLVALGDAHLGRSHLAHLRDERGRNVREEDFLRSFTWGVDET
                     IRREPDGFLWLGDIFDHARPSYRTFAQVLAGLQRLTDAGLHGVAISGNHDTPRIRGTG
                     SPYDALERVFPGVAFAWRMEAVRADVGGVAVHAVPQTLAVDDLREQLKVASGAVRA"
BASE COUNT          451 a         1171 c         1271 g          520 t
ORIGIN      
        1 gaggtcggct tcccagctcg ccaatccttc gccatctcct tcacgcgggt ccgcgttcgt
       61 ctccaacgaa ccgatcggtt cgacggcctc ctacacgtct agggtcgtat cggcaggtcg
      121 gcgagcccgt tggagtcccc cggagggggg atgagtcggg aacgactacc tcggtacgtc
      181 tgcagggacc ggctccctcg ggacgtccga tgaccgcggt accctgcgtg gcgtggcgga
      241 ggcgcaggca cgggggggac cggctcgcgt gctcagcctc gacctcgtcg agcgtgaccg
      301 cctggacgcg gccgcctcgt tcgtggtgga cctcctcgag cggccggcct cggagatccg
      361 ggtgctcgag ggtcagctcg gcctcgacga tgcgctcgtc gtgcccgggc atcccgatgc
      421 gttcgagctc tcgccggtca ccccggtcga accgggcacg gtctgggcgg tggacggggg
      481 gtcgtgcatc gtggcggacg ggcggtcgtt ccaggtcgcg gcgttccggg ccgcccggat
      541 ccggttcgag gacggacaga ccgtcgacgt cgagcggccc cagctcgacg ttcgcgccgt
      601 ggcccccgag gagatggcga ggctcgccag gaccacgctc accgagctct gcggctccga
      661 acccgatcgc ctgccggagc tgccgcgtcc ggtggacgcg ttgcgcgagt gggccgagtg
      721 gggagcggtg gggcgaacgg tccgcgaggc ggctcccggg gacctggtgt tggtggacgg
      781 ctcgctccac gggggaccgc tggtgccgga gcgggtcgtg ctccgcgtgc acgaggaggc
      841 cgtggcgcgc cgcgtgtcgc tcgcgggcgt ggtcaaggca tcgactctgt actgggggcg
      901 gaacgcgccg ctcgtcacgc tgctcaagcg gcgcggcgac cgggaggtcg ggccgtcgcg
      961 gtgggcgacg cggatctcga cggatccggt gttcggccgg ctgtacgtgg gcgacatctt
     1021 cgtcgcccac ctcgccccga ccgcgtcgca cgcgttccgg gtggacgtcg cgcgcgggcc
     1081 ggcgccggcg ctccaggtgc tgggccggct cgccgggctc gcggacgacc ccgcgttcgt
     1141 cgggtacccc tacccgctcg cccgggcgca ccaggcggcg cgagtgacgg gctacgacgt
     1201 ggtggacgtc cgccgagcgt tccgggaggc gctctcgcgt cgcgggatgg ccgaggacga
     1261 cgtcgagctg ctgttccagg acttccacga ggtcttgaac cggtgacggg gggcgcgggg
     1321 ggctcgagga gcgagcgcga acgcgcgagc ggtagccccc cgtttggagg gggcgcgggc
     1381 tacaagggcc ggatcttcgg gaggaacgtc ctcgagtcga cgttccgctc cttctacggg
     1441 acggacctgt tcctcggcga gctgctcgcg gccgaggatc ccgagcgggg ccgccgcttc
     1501 ctgttccgcg tgatccaggt gacgtacggg tcggattcct cggatccctc gtggtccgag
     1561 cgcaccgcgg gctcgtacat gtcgggcgac gtcgccggga ccgagtacac ccttcgagac
     1621 cccgaacagc ggctgtacaa ggtcgcgacg tgccagccgc tcgggtacat cgacgcggcg
     1681 ggcgcgttcc gaaagccgaa gtccctcccg gcccagttcg cgagggtcac ggcgccgacc
     1741 gccgaggact tcgacttcct gcgcgagcgc atgggcgacg tgcgggtggg cctgctccgc
     1801 tccggcgagg acaccatcga tctcgagatc ggcgttcagg gcgacacgct cacgtcgcac
     1861 gtcggcgtct tcgcgaccac gggcatgggc aagtcgaacc tgatgaaggt gctcgcgggg
     1921 cagatcctcg cgtcgcaggg ccggtacgcc tgcctgctgt tcgatccgca cggcgagtac
     1981 ctcgagggcg ggagcgggac gcgcaagggg ctgcgctcgc acccgtgggc gcccgatcgg
     2041 ctgaaggtct acgcgacgcg tgccgcgggg gcgggcgcga cctcgctccg actctcgctc
     2101 gccgagctca cccaggggga cctgcagacc gcgtacgagt ggagccacgc ccaggaggac
     2161 gcgatctacc ggctgatccg gtacttccac gaggagtgga tcgtccgtgt gggcgaggag
     2221 caggacgtcg cgtcgctcgc cgaggacctc ggcgtcgcca ccagcacgat gcaggtgatc
     2281 cagcggcgcg cgcaacggat cctcgacatg ccgtgcatct cgcgcgatcc gtcgcagccc
     2341 tcgctgtcgg cgcgcatcgt cgacgaactg atggacgggt tcagcgtgct cgtggacacg
     2401 agcgggctct cgtcgatgga ggagatcctc gtcgcgtcgg tcatcacgcg gaccgtgctc
     2461 gatgcgtacg cgaacctgta cctggaggac cgagaccggt tcgcggcgct gccgcccacg
     2521 ctggtcggtc tcgaggaggc ccagcgcgtg ctgtcgcgcg tcgctcgctc cgacgagaac
     2581 gtgttcccgc ggctcgcccg ggaaggacgc aagttcaagg tcgggctgtg cgccgtgtcc
     2641 cagcagccga agctgatcga cgacgagctg ctctcgcagt tcaacacgtt cttcgtcctc
     2701 ggcctcgccg acgagaagga ccggaacatc ctgcgcggca gctccaagca ggacatctcc
     2761 gacctcggtc ccgagatcca gacgctgatg ccgggcgagg cgatcgtgac caacctcgag
     2821 tcgcccttcg cgctgccggc gcagatctac ctgtacgagg actacctgaa gtcggtcgcc
     2881 ggaccgtcgc cgctgccgcc ccggccctcg gacgcgtcga aggggttcgc cgagtagatg
     2941 gcgcggctcg tcgcgctcgg ggacgcgcac ctcgggcgtt cgcatctcgc gcacctgcgg
     3001 gacgagcggg gccgcaacgt ccgggaggag gacttcctac gctcgttcac gtggggggtc
     3061 gacgagacga tccgccggga gcccgacggg ttcctgtggc tcggcgacat cttcgaccac
     3121 gctcgcccgt cctatcggac gttcgcgcag gtcttggccg gcctgcagcg gctcaccgac
     3181 gccggccttc acggggtcgc gatcagcggc aaccacgaca ccccgcggat ccgtgggacg
     3241 ggctcgccct acgacgcgct cgaacgcgtc ttcccgggcg tcgcgttcgc gtggcggatg
     3301 gaggcggtcc gcgccgacgt cgggggggtc gccgtccacg cggtgccgca gacgctcgcc
     3361 gtcgacgatc tgcgcgagca gctgaaggtg gcctcgggcg cggtccgggc gga
//
LOCUS       DATBRK010000139         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_884402, whole genome shotgun sequence.
ACCESSION   DATBRK010000139 DATBRK010000000
VERSION     DATBRK010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884402"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VIH70_04225"
     CDS             complement(<1..436)
                     /locus_tag="VIH70_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HEY8115600.1"
                     /translation="MAFDMGQVQRGARNRRTPYHEAAQKHDPKGYTVYNHMYFPIRFD
                     SFESEFETLLNGVTLWDVAVERCLEIGGPDGFRFAQLLTPRDLSTCAVGQGKYVLICD
                     SDGGIINDPVLTRMDETTFWFALASSDALLFARGLKNAYRDLD"
     gene            complement(537..2219)
                     /locus_tag="VIH70_04230"
     CDS             complement(537..2219)
                     /locus_tag="VIH70_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY8115601.1"
                     /translation="MDPNGTANLAFVNGRVYTMDAARRWVGAVAVSQGRIVAVGTDAD
                     VRELIGPGTDVVDLGGRMLLPGFQDAHIHAAASGLEMLRCNLSDAYSLPEYERVVREY
                     AAAHPDDAWIVGGGWSMDVFPSGNPPTEPLDRVVADRPVRLWSRDGHSVWVNSRALEV
                     AGVTRDTPDPVDGWIVRDEDGAPTGTLHEGAIDLVEKHVPEPSAQDRIDGLRTGQTYL
                     HSLGITAWQDAIVGGHDPTFDTYVSFARSGELTARVVGALWWERHRGIEQVEDLIALR
                     ERGRAGRFRATSVKIMQDGVIENLTAAVLEPYLDADGRPTGNRGKSFVDPEELKTVVT
                     RLDAEGFQVHVHAIGERAVREALDAIELARRTNGTNDHRHHIAHIQVIHPDDLPRFRE
                     LGVVANAQPLWAVNEGQMLHLTIPFLGAERSSWQYPFGSLVRSGATLAMGSDWAVSSP
                     NPLWEMHVAVHRTAPSTYEYGGGTGEPFLPDERIDLPTALAGFTINSAYVNHLDHLTG
                     SVEVGKRADLAVLDRDLFAHPLEEIADASVVLTFVDGERVYDAEAGPTSGGR"
     gene            complement(2347..>2985)
                     /locus_tag="VIH70_04235"
     CDS             complement(2347..>2985)
                     /locus_tag="VIH70_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8115602.1"
                     /translation="AGAIVLEAEALAKGYGGPPVFEGIGFHVARGERLLIMGLNGAGK
                     TSLLKILADVTTADAGWFRLGHGVSLGYYAQEHEGIRDGEPVLAHVRAESVDASDQDL
                     RGLLGMFGLIGRVSFQDAGTLSGGEKTKLALAQLVAGRRNLLLLDEPTNNLDPPSRTA
                     VAEALAGWSGSMVLVSHDVGFVQALSPHKVLMMPEGELALWDDELLELVALA"
BASE COUNT          437 a         1096 c          999 g          453 t
ORIGIN      
        1 cgtcgaggtc ccggtacgcg ttcttcagcc cgcgggcgaa caggagcgcg tccgaggacg
       61 cgagggcgaa ccagaaggtg gtctcgtcca tccgagtgag gaccgggtcg ttgatgatgc
      121 ccccgtcgct gtcgcagatc agcacgtact tgccctgccc cacggcgcag gtcgacaggt
      181 ctcgcggcgt caggagctgg gcgaaccgga acccgtcggg gccgccgatc tcgaggcagc
      241 gttcgaccgc gacgtcccag agcgtcacgc cgttgaggag cgtctcgaac tcggattcga
      301 acgagtcgaa ccggatcggg aagtacatgt ggttgtagac cgtgtagccc ttcgggtcgt
      361 gcttctgcgc ggcctcgtgg tacggcgtgc ggcggttgcg tgccccgcgt tgcacctggc
      421 ccatgtcgaa cgccatcgcg gaacctcctc ggctcggtgg gtgcgtcggc ccgatcgggc
      481 cgcggctatc gtgcgtccgg ccccgggacc tggcaacgag cccccatgac cgttggtcag
      541 cggccacccg acgtcgggcc agcctccgcg tcgtacacgc gctcgccgtc gacgaacgtg
      601 agcacgacgg aggcgtcggc gatctcctcg agcgggtgcg cgaacaggtc ccggtcgagc
      661 accgcgagat ccgcacgctt cccgacctcg accgaccctg tgagatggtc gaggtggttc
      721 acgtaggcgc tgttgatcgt gaacccggcc agcgccgtgg gcaggtcgat gcgctcgtcg
      781 ggcaggaacg gctcgcccgt cccgcccccg tactcgtacg tgctcggggc ggtccggtgc
      841 acggcgacgt gcatctccca cagagggttg gggctggaga ccgcccagtc gctccccatc
      901 gcgagggtcg cccccgaccg taccaggctc ccgaacgggt actgccagga cgaccgctcc
      961 gcacccagga acgggatcgt gaggtgcagc atctgcccct cgttgaccgc ccacagcggt
     1021 tgggcgttcg cgacgacgcc gagctcgcgg aagcgaggca ggtcgtcggg atggatcacc
     1081 tggatgtgcg cgatgtgatg tcggtgatcg ttcgtaccgt tcgtccggcg agccagctcg
     1141 atggcgtcga gcgcctccct gacggcccgc tcgccgatcg cgtggacgtg tacctggaac
     1201 ccctcggcgt ccaggcgcgt cacgaccgtc ttgagctcct cggggtcgac gaacgacttc
     1261 ccgcggtttc cggtcgggcg cccgtcggcg tcgaggtagg gctcgaggac ggcggccgtg
     1321 aggttctcga tcaccccgtc ctgcatgatc ttgacggacg tcgcgcggaa ccggccggcg
     1381 cggccgcgtt cgcggagcgc gatcaggtcc tcgacctgct cgatgccgcg gtgccgctcc
     1441 caccacaagg ctccgacgac ccgggcggtg agctcccccg aacgtgcaaa cgacacgtag
     1501 gtatcgaacg tcggatcgtg gccgccgacg atcgcgtcct gccacgccgt gatccccagg
     1561 gagtggaggt aggtctgccc ggttcgcagg ccgtcgatcc ggtcctgggc cgagggctcc
     1621 ggcacatgct tctcgaccag gtcgatggca ccctcgtgga gggtcccggt cggcgccccg
     1681 tcctcgtcgc gaacgatcca cccgtcgacc ggatccggcg tgtcccgggt cacccccgcg
     1741 acctcgagcg cgcgcgagtt cacccacacg ctgtggccgt cgcgactcca caggcgcacc
     1801 ggtcgatcgg cgacgactcg atccaacggt tccgtcggcg ggttcccgga cgggaacacg
     1861 tccatcgacc agccgccgcc cacgatccac gcgtcgtcgg gatgcgccgc cgcgtactcg
     1921 cgcacgacgc gttcgtactc gggcagggag tacgcgtcgg aaaggttgca gcgcagcatc
     1981 tccaggccgc tcgcggccgc atggatatgg gcgtcctgga acccgggcag gagcatcctg
     2041 ccccccagat ccacgacgtc cgtccccgga ccgatcagct cgcggacgtc ggcgtcggtc
     2101 cccacggcga cgatccgccc ctgcgagacc gcgaccgcgc ccacccagcg acgggcggcg
     2161 tccatcgtgt agacgcggcc gttcacgaac gcgaggttcg cggttccgtt cggatccaca
     2221 ccctggtccc ttcccggtcg ctcctctcga agacggagcg cgcagagcat agcgagcgtc
     2281 ccctggccaa cgccatcgcc gaccgccgct ccggtgccga tcctccccgc ccgttcccgc
     2341 gtatggctac gccagggcaa cgagctcgag cagctcgtcg tcccagagtg cgagctcacc
     2401 ctcgggcatc atcaggacct tgtgcggcga gagcgcctgc acgaacccga cgtcgtggct
     2461 caccagcacc atcgaacccg accaacccgc gagcgcctcc gccaccgccg tccgcgacgg
     2521 cggatccagg ttgttggtcg gctcgtcgag tagcaggagg ttccgccgcc ccgccacgag
     2581 ctgcgccagc gcgagcttcg tcttctcccc accggagagc gtgccggcgt cctggaacga
     2641 gacccgaccg atgagcccga acatcccgag caggccgcgg aggtcctggt ccgaggcatc
     2701 caccgactcg gcgcggacgt gcgcgagcac cggctcgccg tcgcggatgc cctcgtgctc
     2761 ctgcgcgtag tacccgagcg agacgccgtg cccgagtcgg aaccaccccg cgtccgcggt
     2821 cgtgacgtcc gcgaggatct tgagcaggct cgtcttgccg gcgccgttca gacccatgat
     2881 cagcagccgc tcaccccggg ccacgtggaa cccgatgccc tcgaacaccg gcgggccgcc
     2941 gtaccccttg gcgagcgcct cggcttcgag cacgatcgct cccgc
//
LOCUS       DATBRK010000140         6081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2185210, whole genome shotgun sequence.
ACCESSION   DATBRK010000140 DATBRK010000000
VERSION     DATBRK010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185210"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VIH70_04240"
     CDS             <1..667
                     /locus_tag="VIH70_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY8115603.1"
                     /translation="RARWVERLSSGEPFDELEGLAMLADYGVPVVESIRADTLEDAIE
                     AAERLGFPVAVKTAAPGLQHKSDVGGVRLGVDDSPSLEDAYTDLERELGPRVTVSRMA
                     PPGIEIAVGIVRDDQFGPLVLVGAGGVLVEIMRDRRLALPPLDEARARSLVDGLEIRP
                     LLDGVRGRPAADADALAKSVVSVSWLAHDLGGDLQALDANPVVCSAEGCVAVDTLVIP
                     RIR"
     gene            complement(677..4936)
                     /locus_tag="VIH70_04245"
     CDS             complement(677..4936)
                     /locus_tag="VIH70_04245"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY8115604.1"
                     /translation="MEFRILGSIEVVENGGGPIALGGPKQRAVLAHLLLRANHLVPTD
                     VLIDEVWGEEPPETVRNALQSYASHLRKALGAERLEGSRAGYVLKAEPSELDAVRFRS
                     LLRDARRILPIDAKAAVDAFDHALDLWRGPAFGDLATEPSLRSEAAQLDDLRLAAIEE
                     RIEAQLELGQLGEVIGELEGLTARHPLRERFWEQLMLALYRAGRQGEALAAYDRARAN
                     LADELGIDPSPELRKVQARILAQSADLDPGGEPLRGYRLLERVGEDPFGVVYRATQPN
                     VGREVAVRVVHEHRANDPAFVRRFEPEAQAVAALEHPHIAPVYDYWREPGRAYVVTRF
                     LRGGSLSELVDRSGALPHERATRILEQVASALAMAHRRGVVHGDLGPLNVLLDEEGNA
                     YLTDFSIGQGSARTSDDLEAFAALARGTLGERLPMDVGEALRRVEIAPGPDDSAAVLA
                     EFASVLGARPDGPAAVVADVRNPFKGLRPFLEADALDFFGREAFIRRLLDRLSDADRS
                     SRFIAVVGPSGSGKSSAVSAGLVAALRHGAVPGSEGWFVTEMHPGHHPMEELDAALLR
                     VAVHPPAGLLGRLESGPRGLLELADALVPEGTELLLIVDQFEEAFTLTEVEDDRALFL
                     ESLRVATADPASRVRVIATLRADFYDRPLRYPRMGEVIGSSTETLSPLTPEELERAIV
                     RPAEHAGLTVDRALVPQIAADVAEQPGALPLVQYALTELYDRRHDGRLTLEAYREIGG
                     VGGALAASAEHLYASRQPAGRNAVRQLFLRLVTIGDGVADTRRRVSLSELSAIEVDAS
                     AMESALDTYGRHRLLTFDRDPVTREPTVEVAHEALLSAWERLRGWIEEAREDVRMRRR
                     LSDTAREWERNGRESSFLLAGSRLDQFESWGTATNLALGLEERGYLSASVARRDEERD
                     AEATRALRERTLERRSVKRLRALVVALTAAALVAGTLTAIALNRNASAQRASSLATGR
                     ELLAGAAKNLEADRQLAILLALEALRTTQGVDGTVLPEAEEFLRRAAPAVAIDTTTIL
                     GSTFRENDGWVTPDGNAVALVGDDGSVGVWNLRNGSRILGRPSPDNSCGAEIGCPDVF
                     HVSLSDDAHLMATGDGEGLAHVWDLDSRREVMTASTHAGPKILAGSFGDGVSPMVALS
                     PDGRLLATLGSDTSLRMWEVSTGARVWALAPEGTDAFGPPRFSADGTRLLVCSWPGPP
                     RVVDVATGETMVSPSGDFGCGTPFSADGSRIAFAVSRGGPDNIEVWDLRQRMRVAAAP
                     YAEIGSAYGVAVVQRMALSPDGTILAVVGSRARNVVLLRDADTLAPLDTLEQRAAVFQ
                     VTFSTDGTRIVTSSDDGAARVWNAQTGRLIFTSPDEPSDLLGAAFSKDGSRIAMVYSD
                     GRILVHAIAFEDALEIAKGRVTRSLTDAECRTYLHVPACPT"
     gene            5109..5684
                     /locus_tag="VIH70_04250"
     CDS             5109..5684
                     /locus_tag="VIH70_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115605.1"
                     /translation="MRTIGRLGGSILALAALAGGWSLAARQASGVELQPGVTVFSEGP
                     AQQQLAEWAVARFERAGLDPPTVEIAFHADASGCGGHLGYAKDGHVDVCTVLVNEMAR
                     RNLLHEMGHIWIDQNVSRADRDRFLEFRRLRTWNASTADWDDRGYEQGAEIMAWALGD
                     RILTAQVPDNDPAQLAVAFEILTGVVIPLGV"
     gene            complement(5745..>6081)
                     /locus_tag="VIH70_04255"
     CDS             complement(5745..>6081)
                     /locus_tag="VIH70_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009070510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8115606.1"
                     /translation="PPPEVDDLGFGFRVPMLVMSPYAKKGYVDDAEGEFSSPLRFVAD
                     NWGLPYLTERIERTHNFEHAFAFRKNPRTDAQPLRPIPHCYGKPSEYPGDDYPGWPEG
                     TQPDPSHFV"
BASE COUNT          909 a         2077 c         2127 g          968 t
ORIGIN      
        1 gcgcgcgcga tgggtcgagc ggctgtcgag cggggaaccg ttcgacgagc tcgaggggct
       61 cgcgatgctc gccgactacg gtgtgccggt cgtcgagagc atccgggcgg acacgctcga
      121 ggacgcgatc gaggcggcgg agcgccttgg gttcccggtc gcggtgaaga cggcggcacc
      181 tggtctgcag cacaagtccg atgtcggcgg cgtgcgcctc ggggtggatg actcgccctc
      241 gctcgaggac gcgtacacag acctcgaacg cgagctcggt ccgcgggtga ccgtgagccg
      301 gatggcgccg ccaggcatcg agatcgcggt cgggatcgtg cgagatgacc agttcggccc
      361 gctcgtgctg gtgggggccg gcggcgtcct ggtcgagatc atgagggacc gtcgcctggc
      421 gctcccaccg ctcgacgaag ctcgggcacg atcgctcgtc gacgggctcg agatccggcc
      481 gctgctcgac ggcgtccggg gccggccggc cgccgacgcc gacgctcttg cgaagtcggt
      541 ggtctcagtg tcatggctgg cgcacgacct cggtggtgac ctccaggccc tcgacgcgaa
      601 ccccgtggtc tgctcggccg aaggttgtgt cgccgtcgac acgctcgtga tcccgcgcat
      661 ccgctgaccg aaccggtcat gtggggcacg cgggaacatg gaggtacgtg cggcactcgg
      721 cgtccgtgag cgagcgcgtg acacgcccct tcgcgatctc gagcgcgtct tcgaaggcga
      781 tcgcatgcac gaggatcctt ccatctgagt agaccatcgc gatcctcgat ccgtccttgc
      841 tgaacgcggc tcctagcagg tcgctcggct cgtcgggcga cgtgaagatc agtctcccgg
      901 tctgcgcgtt ccacactcgg gccgcgccgt cgtcactaga ggtcacgatt cgcgtgccgt
      961 ccgtgctgaa cgtgacctga aagacggctg ccctctgctc cagcgtgtcc agcggggcga
     1021 gcgtgtccgc gtccctcaac aggacgacgt ttcgtgcacg actaccgacc acggcgagaa
     1081 tcgtcccgtc cgggctgagc gccatcctct gaactacggc tactccgtat gccgagccga
     1141 tctcggcata cggagcagcc gcgaccctca ttcgctgccg caggtcccag acctcgatgt
     1201 tgtccgggcc acctctgctc actgcgaagg cgatccggga gccgtcggcg ctgaatggag
     1261 tgccgcaccc gaagtcgccg cttggggaga ccatcgtctc cccggtggcg acgtccacga
     1321 cacggggagg ccccggccag gagcacacga ggagtctggt cccatcagca ctgaagcggg
     1381 ggggaccgaa cgcgtcggtc ccctccgggg ccaaggccca cacccgtgcg cccgtcgaga
     1441 cctcccacat ccgaagcgac gtgtcgctcc ccagggtcgc gagcagacgt ccgtctgggc
     1501 tgagggccac cattggggaa acgccgtcgc cgaaactgcc agccaggatc ttggggcccg
     1561 cgtgcgtcga cgcggtcatg acctcgcgac gggagtcgag atcccacacg tgcgccagcc
     1621 cttcgccgtc tccggtcgcc atcaggtggg cgtcgtcgct caggctgaca tggaagacgt
     1681 cggggcaacc gatctcggcg ccgcaggagt tgtcggggga ggggcggcca aggatccgcg
     1741 agccgttccg aagattccat accccgaccg atccgtcatc gcctaccagc gcgacggcgt
     1801 tgccgtcggg cgttacccag ccgtcattct cgcggaacgt actacccagg atcgtggtcg
     1861 tgtcgatggc gacggccggg gcggcgcggc gcaggaactc ctcggcctcc ggcaggaccg
     1921 ttccatcgac gccctgtgtc gtccggagcg cctcgagggc gagcaggatg gcgagctgtc
     1981 gatcggcctc gaggttcttc gccgccccag cgaggagctc acggcctgtg gccaggctcg
     2041 acgcacgctg cgcgcttgcg ttccggttca gggcgatcgc cgtcaacgtt cccgccacga
     2101 gcgcggcggc ggtgagcgcg acgacgagtg cccgcagtcg cttgacggag cgacgttcaa
     2161 gggtgcgttc gcgaagggcc cgggtcgctt ctgcgtcgcg ctcctcatct cgcctggcca
     2221 cgctcgccga aaggtagccg cgctcctcga gcccgagcgc caggttggtg gccgttcccc
     2281 acgattcgaa ctgatccagg cgggatcccg cgagcaggaa gctcgactcg cgtccgtttc
     2341 gctcccattc acgggctgta tccgacagcc gccggcgcat ccggacgtcc tcgcgggcct
     2401 cctcgatcca tccgcgcagc cgttcccagg cgctcaggag cgcttcgtgc gccacctcga
     2461 cggtcggctc tcgagtcacg gggtcgcgat cgaacgtgag gaggcggtgg cgcccgtagg
     2521 tgtccagggc cgactccatc gcgctcgcat cgacctcgat cgcggatagc tcggacagcg
     2581 agacccggcg ccgcgtgtcc gcgactccat cgccgatcgt cacgagccgc aggaagagct
     2641 ggcgaacggc gtttcggcct gctggttgtc ggctcgcgta cagatgctcg gcgctcgcgg
     2701 cgagggcgcc tccgacgcca ccgatctcgc ggtacgcctc gagcgtgagg cgcccgtcgt
     2761 ggcgtcgatc gtagagctcg gtgagagcgt actggaccag gggcagcgcg cccggctgct
     2821 ccgcgacgtc cgcggcgatc tgggggacga gcgcgcgatc gacggtcaag cccgcgtgct
     2881 ccgccggccg gacgatcgcc cgttcgagct cctcgggcgt caaggggctc agggtctcgg
     2941 tactcgaccc gatcacctct cccatccggg gataccggag cggccggtcg tagaagtcgg
     3001 cgcggagcgt ggcgatcacg cgtacccggc ttgccgggtc ggcggtcgcc actcggaggc
     3061 tctcgaggaa cagcgcccgg tcgtcctcga cctcggtgag cgtgaacgcc tcctcgaact
     3121 gatcgacgat cagcaggagc tcggtgcctt cggggacgag cgcgtccgcc agttcgagga
     3181 ggcccctggg acccgactcg aggcggccga ggaggccggc gggcggatgc acagcgaccc
     3241 gcagcaacgc ggcgtcgagc tcctccatcg gatggtgacc cggatgcatc tcggtaacga
     3301 accagccctc tgagccgggg accgcaccgt ggcggagtgc cgccaccagg ccggcgctga
     3361 ccgccgagga cttgccgctt ccgctcggtc cgacgaccgc gataaacctc gacgatcggt
     3421 ccgcatccga caggcgatcg agcagccgcc ggatgaatgc ttccctgccg aagaagtcca
     3481 gggcgtcggc ttcgaggaac ggtcgcagac ccttgaaggg gttgcggacg tcggcgacga
     3541 cggcagccgg gccgtccggc cttgccccga ggaccgacgc gaactcggcg aggaccgccg
     3601 cgctgtcgtc gggtcccggc gcgatctcca cccggcggag cgcctcaccg acatccatgg
     3661 ggagtcgctc gccaagcgtc ccgcgcgcca aggcggcgaa cgcttcgaga tcgtccgacg
     3721 ttcgcgccga tccctgtccg atggagaagt ccgtgaggta ggcgttcccc tcctcgtcga
     3781 ggagcacgtt caacgggccg agatccccgt ggaccacgcc ccgacggtga gccatcgcca
     3841 aggcggaggc gacctgctcg aggatccggg tcgcccgttc gtgcggtagc gcgccagagc
     3901 gatcaaccag ctcgctcaag cttccacctc ggaggaacct ggtcacgacg tacgcccgac
     3961 ccggctcgcg ccagtagtcg taaacgggcg cgatatgggg atgctcgagc gccgcgactg
     4021 cctgcgcctc gggttcgaag cggcgaacga acgccggatc gttggcacga tgctcgtgta
     4081 ctacccggac cgcgacttcc ctgccgacgt tcggctgcgt cgctcggtag acgacgccga
     4141 acgggtcctc tccgacccgt tcgagcaatc ggtacccccg cagcggttct ccgccgggat
     4201 ccaggtccgc gctctgggcg aggatccgcg cctgcacctt gcgcagctcg ggtgacggat
     4261 cgatcccgag ctcgtcggcg aggttcgcgc gagcccggtc gtaggccgca agcgcttcgc
     4321 cttgtcggcc cgcacggtag agagcgagca tcagctgctc ccagaagcgt tcgcggagcg
     4381 ggtggcgtgc cgtgagacct tcgagctcac cgatgacctc accgagctgc ccgagctcga
     4441 gctgcgcctc gatccgctcc tcgatcgctg cgagccgcag gtcgtcgagc tgcgctgctt
     4501 ctgatcggag cgacggttcg gtcgcgagat ccccgaaggc ggggccgcgc cacagatcca
     4561 gcgcgtggtc gaatgcatcg acggccgcct tcgcgtcgat gggcaggatc cgccgcgcgt
     4621 cgcgaaggag cgaccggaag cgcacagcat cgagctccga tggttcggcc ttgagcacgt
     4681 agccagccct gcttccctcg agccgctcgg cgcccagcgc cttgcgcagg tgcgaggcat
     4741 agctctgcag ggcgttccga acggtctcgg gcggctcctc gccccatacc tcgtcgatca
     4801 ggacgtcggt cggtaccaga tggttggcgc gcaggagcag gtgggcaagg accgcccgct
     4861 gtttgggtcc cccgagagcg atcgggccgc caccgttctc gacgacttcg atcgacccca
     4921 ggatccggaa ctccatcggg cgacggtagc ccctggtcgc cgagatggga atgccggatt
     4981 ccgccgacgg gcgagcgttc accgctgcag cgtctgcgag gtcgctgaaa gcttgctgaa
     5041 aggttcgggt cagcgggtga cacgccttcc gcatcgggcg ctcatgcgcc cggttcgagc
     5101 gtgcgcccat gcgcacgatc ggtcggctcg ggggttcgat cctcgctctg gcggcacttg
     5161 ccggaggatg gtccctcgcg gcgcggcagg catccggggt ggagctccag ccaggggtca
     5221 cggtcttctc ggagggccca gcccagcagc agctcgccga gtgggcggtg gcgcggttcg
     5281 agcgcgcggg gttagacccc ccgacggttg agatcgcctt ccatgcggac gcctccgggt
     5341 gcggaggcca cctcgggtac gcgaaggatg gtcatgtcga cgtctgcaca gtgctcgtga
     5401 atgagatggc tcgcaggaac ctcctgcacg agatggggca catctggatc gaccagaacg
     5461 tgtcgcgtgc ggaccgcgat cgtttcctcg agttccgccg cctgcggacg tggaacgcgt
     5521 cgacggctga ttgggacgac cgtggctacg agcagggagc cgagatcatg gcgtgggctc
     5581 tcggtgacag gatcctgacg gcgcaggtcc cggataacga tcctgcgcag ctcgcggtcg
     5641 cgttcgagat cctcaccggc gtcgtcattc cccttggcgt ctgaaaacgc gacttacgct
     5701 catcccccgc gatggaccgg atgcgagccg gggtctgtcg acgatcagac gaagtggctc
     5761 gggtccggct gcgttccctc gggccacccg gggtagtcgt cgccggggta ctcggacggc
     5821 ttgccgtagc agtgggggat gggccgtaaa ggctgtgcgt cggtccgcgg gttcttccgg
     5881 aacgcgaagg cgtgctcgaa gttgtgggtg cgttcgatcc gttcggtcag gtacggcagc
     5941 ccccagttgt ccgcgacgaa ccggagcggg ctcgagaact cgccctcggc gtcgtcgacg
     6001 tagcccttct tcgcgtacgg gctcatcacg agcatcggga cgcggaaccc gaacccgagg
     6061 tcgtcgacct cgggcggcgg c
//
LOCUS       DATBRK010000141         8855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2185288, whole genome shotgun sequence.
ACCESSION   DATBRK010000141 DATBRK010000000
VERSION     DATBRK010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185288"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VIH70_04260"
     CDS             complement(<1..381)
                     /locus_tag="VIH70_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY8115607.1"
                     /translation="MSLKIKVPLAWFGPGTLVRHAAVVCEGGRVTYAGPSAAAPPADD
                     FREVDGFLMPGVADRHVHIRLADPGAVLLGGVTAVRDLAWAPDEIFALANASELPTFT
                     GPVIRAAGPMLTAAGGYPTSASWAP"
     gene            complement(392..2404)
                     /gene="uvrB"
                     /locus_tag="VIH70_04265"
     CDS             complement(392..2404)
                     /gene="uvrB"
                     /locus_tag="VIH70_04265"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY8115608.1"
                     /translation="MPPFKIHADHVPTGDQPTAIDQLVDGVRAGEPHITLLGATGTGK
                     TFTIANVVERVQRPTIVIAPNKSLAAQLANEFREAFPENAVEYFVSYYDYYQPEAYVP
                     STDTYIEKDSSINDEIERLRHSATAALLTRRDVLIVASVSCIYGLGSPEEYEGQILRV
                     HKGVATDLEWSKQRLVDIQYLRNQANLARGKFRVQGDVLEVHPSYEETAIRIEWWGDT
                     IERITRFDPLTGEMLGDLNEITVFPASHYVASDERMKRAVVSIEEELHDRLALLESQS
                     KLLEAERLRMRTTYDLEMLREVGFCSGIENYSRHIDGRAPGEAPYTLLDYFPDDWLCV
                     IDESHVTVPQIHGMYEGDKTRKETLVEFGFRLPSALDNRPLRFEEFVKKVNQTVYMSA
                     TPGPYELRTSIRTAEQIVRPTGLVDPEVQVRPTRGQVDDLIEEIRKRAEAGQRVLVTT
                     LTKKMAEDLTDYLLEMGIRVRYLHSEVDTIQRIEIIRDLRLGEFDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSDTSLIQTIGRAARNVDGTVIMYADQVTDSMKQAIGETQ
                     RRRKIQIEFNREHGIDPQSIRKKVSDILLTMAVDTRAPTPEKRRRKREHPEMPTEELE
                     RLIQQLEEEMHEAAKDLRFEYAARLRDEVVDLRKELKALRGAGVGG"
     gene            2411..2920
                     /locus_tag="VIH70_04270"
     CDS             2411..2920
                     /locus_tag="VIH70_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115609.1"
                     /translation="MIRPGADGLAWWRWKSATTVVGVSMSMSADHSDEDRRIHIGDWA
                     TLNGFELLDDLDREAERLPLFVIQTTVDRDVHNVCVGRWEDTDVACFDLRRGSGWDRI
                     DSTGATIAYPYGLPGLLILHPGVADDDRMLSSRPVIRITPWRLRTRCDPTMPRSPSPS
                     STGPSRPGS"
     gene            2955..3083
                     /locus_tag="VIH70_04275"
     CDS             2955..3083
                     /locus_tag="VIH70_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115610.1"
                     /translation="MGTDWLLGYAPQLTVELFPNLLDGVLGFRDRISSDVVAAFGR"
     gene            complement(3129..3881)
                     /locus_tag="VIH70_04280"
     CDS             complement(3129..3881)
                     /locus_tag="VIH70_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY8115611.1"
                     /translation="MAPAAALRIEDLHKTFDRLEVLKGVDLEVDEHEVVGLIGASGSG
                     KSTLLRCINLLEPVNAGRVFIEGEEITARGVDVNRIRQRVGIVFQAFNLFPHMTVLRN
                     VTLGPIEALGESRAQAEALATELLERFGLADKRDDYPDRLSGGQQQRVAIVRALAMKP
                     DVMLLDEVTSALDPELVAEVLNVIRELAAGGMTMVIATHEMGFARDIANRIGFLDAGV
                     ILEEGPPEEIFSSPREERTQRFLQSIIDAGRM"
     gene            complement(3883..4782)
                     /locus_tag="VIH70_04285"
     CDS             complement(3883..4782)
                     /locus_tag="VIH70_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8115612.1"
                     /translation="MIGGESPEKYQPLPGGGYTPPSGDRGSRILAPLRAEGARGVTIA
                     VVSTVVVFGLLAFAILNSSGWPQIKEAFFDPTVFRESLGKIVQRFARNVFIFVVAEVL
                     VLILAMILAVMRSLRGPVFTPLRIMAAAYTDLFRAIPTILVILLLGFGAPALQIKGVP
                     HSDIFWAIVALVLVYSAYVAEVYRAGIQSIHPSQVASARALGLTRGKTMRFVVVPQAV
                     RRVIPPLLNDFIGLQKDSALIAVIGYTEALKEAQILSAATFDFTPYVALALVYIVITI
                     PEARFVDWLVARDQRRLQAVGGV"
     gene            complement(4790..5707)
                     /locus_tag="VIH70_04290"
     CDS             complement(4790..5707)
                     /locus_tag="VIH70_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8115613.1"
                     /translation="MRRTFAFLVIGSLLFAACAEDNPTIGGTGTTGTGGTGSTGPATA
                     ADCAAANADAFQSPGTLTIGTGNPAFPPWWEGGTSDGSEFEFNDPAKGEGYEGAVAAE
                     VATRMGFSFPDQVTFVPVAFNKSFAPGPKDFDFVLQQISYKPERDDAVDFSDSYYNVN
                     QALVSAEGTSVADATTIADLKDAKLGAPIGTTSLDVITEQIQPTQEPAVYDDLAQAVT
                     DLQNGDIDGIVVDLPTAFFMAAVQIKHGVVVGQFGAESDEYFAMAFETGSPLVECVNL
                     ALDEMKADGTLDDIERQWLADKTNAPVLD"
     gene            complement(5784..6560)
                     /locus_tag="VIH70_04295"
     CDS             complement(5784..6560)
                     /locus_tag="VIH70_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8115614.1"
                     /translation="MSDAGLLALQVRTTNKAFWRNPASAFFTFAFPLMFLVIFTSLLG
                     SGTAEVAPGVEIQQDSYYVAAMAAFAVISATYTNLAISIAFTRDAGILKRTRGAPLPA
                     GIYLGARVTHAILISILLVVITTAFGVAFYQADIPSGALLWEHLATVVVGAMAFSALG
                     LATTAIVPNADAAPAVVNAIILPLLFLSGIFIPLGPDAPQWMKVVGDIFPVKHFAEAM
                     LGSFYGPPLPFEWSDVAVVAAWGVVGLALAIRFFSWEPRR"
     gene            complement(6557..7465)
                     /locus_tag="VIH70_04300"
     CDS             complement(6557..7465)
                     /locus_tag="VIH70_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005310713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8115615.1"
                     /translation="MSDRAMEVTGLRKTYGDLEAVAGIDLHVGVGEVFALLGPNGAGK
                     TTTVEILEGYRQRTAGEVSVLGFDPAGRARALRERVGIVLQSTGVDPYLTVRETVALY
                     ASYYPSPRDVDEVVELVGLAEKRDTRVLKLSGGQQRRLDVAIALAGDAELLFLDEPTT
                     GFDPGARRNAWEIVRNLTALGKTVFLTTHYMDEAQYLADRVAVIAKGRIVAEGPPATL
                     GDRHLAKSVIRFRPPAGDLPPEIASIATTANDELELTVTDPTRILHELTSWAVGRGVE
                     LEGLEVSRPSLEDVYLELTGGEAGGE"
     gene            complement(7483..8457)
                     /locus_tag="VIH70_04305"
     CDS             complement(7483..8457)
                     /locus_tag="VIH70_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY8115616.1"
                     /translation="MSARARVAVMGATAEDPPPGIGAIADTVDLAFADDVPSLAGALE
                     GRDVLFAWRPRSALLSGAWGSAADLQWIQSASAGVDNLLFPELAASHVVLTNARGVYE
                     DAIAEYVLGLLLVFAKGLGGVLERQGRREWRHNETERLERKRVLVVGIGPIGRAIARS
                     CAALRMSVRGVARTPRPGDDVLVEIFGTERLADAVRSADYVIDALPATDETHHLFDHV
                     VFEAMEPWTRFVNIGRGSTVDQRALVRALETGAIAGAALDVFEEEPLPEDDPLWAMPN
                     VIVSPHMAGDAAGWREGVVELFVRNLERYLTGRPLLNVVDKTRGYVPS"
     gene            complement(8454..>8855)
                     /locus_tag="VIH70_04310"
     CDS             complement(8454..>8855)
                     /locus_tag="VIH70_04310"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEY8115617.1"
                     /translation="VAGTARGGVMSEERSYDVEEANAILPELRERLARIRDARQVILR
                     EAEVVRERVVADGGGSQAGREYWDASARLRTELEYLAAENILLRDPETGLVDFPAQRD
                     GERIYLCWRVGEEAVANWHPLDTGFSSRRPL"
BASE COUNT         1350 a         3064 c         3049 g         1392 t
ORIGIN      
        1 cggagcccag gaggccgacg tcgggtaccc gcccgcggcg gtgagcatgg gacccgcggc
       61 tcggatcacc ggtccggtga acgtcgggag ctcggaggcg ttcgcgagcg cgaagatctc
      121 gtccggcgcc cacgcgagat cacggaccgc cgtcacgccg ccgagcagca ccgcgcccgg
      181 atccgcgaga cggatgtgga cgtgccggtc ggcgacaccc ggcatcagga acccgtcgac
      241 ctcgcggaag tcgtcggcgg gcggtgcggc cgcgctcggc cccgcgtacg tgacccgtcc
      301 gccctcgcac acgacggccg cgtgtcggac gagcgtcccc gggccgaacc aggcgagcgg
      361 gacctttatc ttcagcgaca tcggcgccgg ttcagccgcc gacgccggcg ccgcgcaggg
      421 ccttgagctc cttgcggagg tcgacgacct cgtcgcgcag gcgtgccgcg tactcgaacc
      481 gcaggtcctt cgcggcctcg tgcatctcct cctcgagctg ctggatcagg cgctcgagct
      541 cctcggtcgg catctcgggg tgctcgcgct tgcggcggcg tttctccggc gtgggagccc
      601 tcgtgtcgac cgccatcgtg agcaggatgt ccgagacctt cttgcggatc gactgcggat
      661 cgatcccgtg ctcgcggttg aactcgatct ggatcttccg acgccgctgc gtctcgccga
      721 tcgcctgctt catcgagtcg gtgacctggt ccgcgtacat gatcaccgtc ccgtcgacgt
      781 tgcgcgcggc ccggccgatc gtctggatca gcgaggtgtc cgaacgcagg aacccttcct
      841 tgtcggcgtc gaggatcgcg accagggaga cctcgggcag gtcgaggccc tcccgcagga
      901 ggttgatccc gaccagcacg tcgaactcgc cgaggcgcag gtcccggatg atctcgatgc
      961 gctggatcgt gtcgacctcc gagtgaaggt accggacccg gatccccatc tcgagcaggt
     1021 agtccgtgag gtcctcggcc atcttcttgg tcagcgtcgt gacgaggacc cgctggccgg
     1081 cctcggctcg cttgcggatc tcctcgatca ggtcgtccac ctgcccccgg gtaggccgaa
     1141 cctggacctc ggggtcgacc agaccggtgg ggcgcacgat ctgctcggcg gtgcggatcg
     1201 acgtgcggag ctcgtagggc cccggcgtgg cggacatgta gacggtctga ttgaccttct
     1261 tcacgaactc ctcgaaccgc aggggccggt tgtccagcgc gctcggcagg cggaacccga
     1321 actcgacgag ggtctccttc cgcgtcttgt cgccctcgta catcccgtgg atctggggga
     1381 ccgtgacgtg ggactcgtcg atcacgcaga gccagtcgtc ggggaagtag tcgagcaacg
     1441 tgtacggggc ctcgcccggc gcacgaccgt cgatgtgccg tgaatagttc tcgatgccgg
     1501 agcagaaccc gacttcgcgg agcatctcga ggtcgtaggt cgtgcgcatc cgcaggcgct
     1561 cggcctccag gagcttgctc tggctctcga gcagcgcgag ccggtcgtgg agctcttcct
     1621 cgatcgagac gacggcccgc ttcatccgct cgtccgaggc gacgtagtga gaggccggga
     1681 agaccgtgat ctcgttcagg tcgccgagca tctcgccggt gagcgggtcg aaccgcgtga
     1741 tccgttcgat cgtgtcgccc caccactcga tccggatcgc ggtctcctcg tacgaggggt
     1801 ggacctcgag cacgtcgccc tgcacgcgga acttcccgcg ggccaggttc gcctggttcc
     1861 gcaggtactg gatgtcgacc aggcgctgct tcgaccactc caggtcggtc gcgacgccct
     1921 tgtggacacg caggatctgg ccctcgtact cctctggcga gcccaggccg tagatgcacg
     1981 agacgctggc cacgatcagg acgtcgcgac gggtcaggag ggcggccgtc gccgaatgcc
     2041 gcaggcgctc gatctcgtcg ttgatcgagg agtccttctc gatgtaggtg tcggtggacg
     2101 ggacgtaggc ctcgggctgg tagtagtcgt agtacgagac gaagtactcc accgcgttct
     2161 cggggaacgc ctcgcggaac tcgttcgcga gctgggccgc gagcgacttg ttgggcgcga
     2221 tcacgatcgt gggccgttgg acgcgttcga ccacgttggc gatcgtgaac gtcttgcccg
     2281 tcccggtggc cccgaggagc gtgatgtgcg gctcgcctgc ccggacgcca tcgaccagct
     2341 ggtcgatggc ggtgggctgg tcaccggtcg gaacgtggtc ggcgtggatc ttgaacgggg
     2401 gcaccagtcg atgatacggc cgggcgccga cgggctcgcg tggtggcgat ggaagtccgc
     2461 caccacggtc gtgggagtat ccatgtcgat gtccgccgac cattccgacg aagaccgccg
     2521 catccatatc ggcgactggg ccacgctgaa cgggttcgag ctcctcgatg acctcgatcg
     2581 ggaggcggaa cggcttccac tgttcgtgat ccagaccacg gtcgacaggg acgtccacaa
     2641 cgtgtgcgtc gggcgttggg aggacacgga cgtcgcgtgc ttcgatcttc gccggggcag
     2701 cggctgggac aggatcgact caaccggtgc gacgatcgcc tacccctacg gacttcctgg
     2761 gctcctgatc ctgcaccccg gggtcgccga tgacgatcgg atgctctcgt cacgcccggt
     2821 gatcaggatc accccctggc gtctgcgtac gaggtgcgat ccgacgatgc cgcgttcgcc
     2881 gtcgccatcc tcgacggggc cctcgcgacc tggctcatga cgtggcgcgg caacctccgc
     2941 cgatgagttt cgaggtgggc accgactggt tactcggcta cgctccgcag ctcaccgtgg
     3001 aactcttccc caacctcctc gacggcgtgc tcgggtttcg cgatcgcatc tcctccgacg
     3061 tggttgccgc cttcgggcgg tgagtatcgc cacccgctcg tctacggtcg gtggatgcaa
     3121 ggagtcgact acatccgccc ggcgtcgatg atgctctgga ggaaccgctg ggtccgctcc
     3181 tctcgcggag aggagaagat ctcctcggga gggccctcct cgaggatcac gccggcgtcc
     3241 aggaacccga tccggttcgc gatgtcgcgc gcgaacccca tctcgtgggt cgcgatcacc
     3301 atcgtcatcc cgccggcggc gagctcgcgg atcacgttca gcacctcggc gacgagctcg
     3361 gggtcgagcg cgctcgtgac ctcgtcgagc aacatcacgt cgggcttcat cgcgagcgcc
     3421 cgcacgatcg ccacgcgctg ctgctgcccg cccgagaggc ggtcggggta gtcgtcccgc
     3481 ttgtcggcga gcccgaaccg ctcgaggagc tccgtagcga gcgcctcggc ctgggccctg
     3541 gactcgccga gcgcctcgat cggtccgagc gtcacattgc ggagcaccgt catgtgcggg
     3601 aagaggttga acgcctggaa gacgatcccg acgcgctgcc ggatccggtt cacgtcgacc
     3661 ccgcgggcgg tgatctcctc gccctcgatg aacacccggc cggcattgac gggctcgagc
     3721 aggttgatgc agcggagcag ggtcgacttg cccgagcctg aggccccgat caggccgacg
     3781 acctcgtgct cgtcgacctc gaggtcgacg cccttcagca cctcgagccg gtcgaacgtc
     3841 ttgtggaggt cctcgatccg cagggccgcg gcgggcgcca tctcacaccc cgcccacggc
     3901 ctggagccgg cgctggtccc gcgcgacgag ccagtcgacg aagcgagcct cggggatcgt
     3961 gatcacgatg tacacgagcg cgagcgctac gtagggcgtg aagtcgaagg tcgcggccga
     4021 gaggatctgc gcctccttga gggcctccgt atacccgatg accgcgatca gggccgagtc
     4081 cttctgcagc ccgatgaagt cgttcaggag cggtgggatg acgcggcgca ccgcctgagg
     4141 cacgacgacg aaccgcatcg tcttgcctcg ggtcagcccg agcgcgcgcg ccgaggcgac
     4201 ctgcgagggg tggattgact ggatcccggc gcgatagacc tcggcgacgt aggccgagta
     4261 cacgaggacg agcgctacga tcgcccagaa gatgtccgag tgcggcaccc ctttgatctg
     4321 cagcgccggc gccccgaacc ccaggagcag gatcaccagg atcgtcggga tcgcgcggaa
     4381 caggtccgtg tacgcggccg ccatgatgcg gagcggcgtg aacacgggtc cgcggaggct
     4441 gcgcatgacg gcgaggatca tggcgaggat caggacgagg acctcggcca ccacgaagat
     4501 gaagacgttg cgggcgaacc gttgcacgat cttcccgagc gactcgcgga agaccgtcgg
     4561 gtcgaagaag gcctccttga tctggggcca cccggacgag ttgaggatcg cgaaggcgag
     4621 cagtccgaac acgaccacgg tgctcacgac ggcgatcgtc acgccccggg cgccctcggc
     4681 cctcagcggg gcgaggatcc tcgaaccgcg gtctccggac gggggcgtat agccaccacc
     4741 gggcaggggt tggtatttct ccggcgactc cccgccgatc accgacccgc tagtccagga
     4801 ccggagcgtt cgtcttgtcg gccagccact gccgttcgat gtcgtcgagc gtcccgtccg
     4861 ccttcatctc gtcgagcgcg aggttcacgc actcgaccag cgggctcccg gtctcgaacg
     4921 ccatcgcgaa gtactcgtcg gactcggccc cgaactgccc cacgacgacg ccgtgcttga
     4981 tctgcacggc cgccatgaag aacgcggtcg gcaggtcgac gacgatgccg tcgatgtcgc
     5041 cgttctgcag atcggtcacg gcctgcgcga ggtcgtcgta gacggccggc tcctgcgtgg
     5101 gctggatctg ctcggtgatc acgtcgaggc tcgtcgtccc gatcggcgcg ccgagcttcg
     5161 cgtccttcag gtcggcgatc gtggtcgcgt cggccaccga ggtcccctcc gccgacacga
     5221 gcgcctggtt cacgttgtag taggagtcgc tgaagtccac cgcgtcgtca cgctccggct
     5281 tgtacgagat ctgttggagc acgaagtcga agtccttggg gccgggcgcg aacgacttgt
     5341 tgaaggccac cggaacgaac gtgacctggt cggggaacga gaagcccatc cgcgtcgcga
     5401 cctcggccgc gacggcgccc tcgtaccctt cgcccttggc cgggtcgttg aactcgaact
     5461 cggaaccgtc ggaggtgccg ccctcccacc agggcgggaa cgccgggttg ccggtgccga
     5521 tcgtcagggt tccggggctc tggaaggcat cggcgttcgc cgcggcgcag tcggcggccg
     5581 tcgcggggcc ggtggagccg gtgccgcccg tcccggtcgt cccggtgccc ccgatcgtcg
     5641 ggttgtcctc cgcgcacgcg gcgaacagca acgagccgat gacgaggaag gcaaacgtcc
     5701 tgcgcatcga tcgaatcccc ctgccgagtg aagtgcgcga gcctagcaca cgcctgttct
     5761 cccgggggac ggggcgcgtg ctctcacctg cgcggctccc acgagaagaa ccggatcgcg
     5821 agcgcgagcc ccaccacccc ccaggcggcg acgacggcca catcggacca ttcgaagggc
     5881 agcggcggac cgtagaacga gccgagcatc gcctccgcga aatgcttcac ggggaagatg
     5941 tcgcccacca ccttcatcca ctgcggagcg tccgggccga gcgggatgaa gatgccggag
     6001 aggaacagca gcggcaggat gatcgcgttc accacggcgg gggccgcatc cgcgttcggc
     6061 acgatcgcgg tggtcgccag cccgagggcc gagaacgcca tcgcgcccac gaccacggtg
     6121 gccaggtgct cccacaggag cgcgccggag gggatgtcgg cctggtagaa cgcgacgccg
     6181 aacgcggtcg tgatgacgac gagcaagatc gagatcaaga tcgcgtgcgt cacccgtgcc
     6241 ccgaggtaga tccccgccgg cagcggtgcc ccgcgcgtgc gcttcaggat gccggcgtcc
     6301 ctcgtgaacg cgatgctgat ggcgaggttc gtgtaggtcg cggagatgac cgcgaacgcc
     6361 gccatggcgg ccacgtagta gctgtcctgc tggatctcga cgccgggcgc gacctccgcc
     6421 gttccgctcc cgagcagcga cgtgaagatc accaggaaca tcagcgggaa cgcgaacgtg
     6481 aagaacgccg aggccgggtt gcgccagaac gccttgttcg tcgtccggac ctggagcgcg
     6541 aggagaccgg cgtcgctcat tcgccacccg cctccccacc cgtgagctcg agatagacgt
     6601 cctcgaggga cggccgggag acctccaggc cctcgagctc gacgccgcgc cccaccgccc
     6661 acgacgtcag ctcgtgcagg atgcgtgtgg ggtcggtgac ggtgagctcg agctcgtcgt
     6721 tcgccgtcgt ggcgatcgag gcgatctccg gaggcaggtc gccggccggc gggcggaatc
     6781 ggatcacgct cttggccagg tgccggtcgc ccagggtcgc cggagggccc tccgcgacga
     6841 tccgcccctt cgcgatcacg gcgactcggt ccgcgaggta ctgggcctcg tccatgtagt
     6901 gcgtggtcag gaacacggtc ttgccgagcg cggtcaggtt ccgcacgatc tcccaggcat
     6961 tgcgccgggc gcccggatcg aagcccgtcg tgggttcgtc caggaacagg agctcggcgt
     7021 cgcccgcgag ggcgatcgcg acatcgagcc gacgctgctg cccacccgag agcttgagca
     7081 cgcgcgtgtc gcgcttctcg gccaacccca cgagttcgac gacctcgtcg acgtcgcggg
     7141 gggacgggta gtagctcgcg tacagggcca cggtctcgcg gacggtcagg tacgggtcga
     7201 ccccggtcga ctgcaggacg atcccgaccc gctcgcggag ggcgcgcgct cgccccgccg
     7261 ggtcgaaccc gaggaccgag acctcgcccg cggtccgttg gcgataaccc tcgaggatct
     7321 cgaccgtggt ggtcttgccg gcgccgttcg ggccgaggag cgcgaacacc tcgccgacgc
     7381 cgacgtgtag gtcgatcccg gcgaccgcct cgaggtcgcc gtaggtcttg cggagaccgg
     7441 tgacctccat cgctcggtcg ctcatcgtcg caggctaccc actcacgacg gtacgtagcc
     7501 gcgcgtcttg tcgacgacgt tcagcagggg gcgaccggtg agatagcgct cgaggttgcg
     7561 cacgaacagc tcgaccaccc cctcgcgcca cccggcggcg tcgccggcca tgtgcggcga
     7621 gacgatcacg ttcggcatcg cccagagcgg gtcgtcctcg ggcagcggct cctcctcgaa
     7681 gacgtcgagc gcggcgccgg cgatggctcc ggtctcgagg gcccggacca gcgctcgttg
     7741 gtcgaccgtg ctcccgcggc cgatgttcac gaacctcgtc cacggctcca tcgcctcgaa
     7801 caccacgtgg tcgaacaggt gatgagtctc gtccgtggcg ggcagcgcgt cgatcacgta
     7861 gtcggccgat cggaccgcgt cggcgagccg ctcggtgccg aagatctcga ccaacacgtc
     7921 gtcgccgggt cggggggttc gagcgacgcc ccggacggac atccggagcg cggcgcagct
     7981 gcgagcgatg gcgcgtccga tcggtccgat gccgacgacg aggacccgct tgcgctcgag
     8041 ccgctcggtc tcgttgtgtc gccactcgcg gcgcccctgc cgctcgagga ccccaccgag
     8101 acccttcgcg aacacgagca gcaacccgag gacgtactcg gcgatcgcgt cctcgtaaac
     8161 gccgcgagcg ttcgtcagga cgacgtggct cgccgcgagc tcggggaaga gcaggttgtc
     8221 gacgcccgcg gacgccgact ggatccattg caggtcggcc gcgctccccc atgccccgga
     8281 gagcagggcg ctgcgcggac gccaggcgaa gagcacgtcc ctgccctcga gcgccccggc
     8341 gagcgacggg acgtcgtcgg cgaacgcgag gtcgacggtg tcggcgatcg ccccgatgcc
     8401 cgggggcggg tcctcggcgg tcgcgcccat gaccgccacc cgggcgcggg cgctcacagc
     8461 ggacgccggc tcgagaagcc cgtgtcgagc ggatgccagt tcgcgacggc ctcctctccg
     8521 acccgccagc agaggtagat ccgctcgccg tcgcgctggg cggggaagtc aacgagcccg
     8581 gtctccggat cgcgcagcag gatgttctcc gcggctaggt actcgagctc ggtccgcagc
     8641 ctcgcgctcg cgtcccagta ctctcgcccc gcctggctcc cgccgccgtc cgcgacgacc
     8701 cgttccctca ccacctcggc ctcccgcagg atcacctgcc gggcgtcgcg gatcctcgcc
     8761 agccgttccc ggagctccgg caggatcgcg ttggcctcct cgacgtcgta cgaacgctcc
     8821 tcggacatca caccaccccg agcagtgccg gcaac
//
LOCUS       DATBRK010000142         8090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_601011, whole genome shotgun sequence.
ACCESSION   DATBRK010000142 DATBRK010000000
VERSION     DATBRK010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601011"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VIH70_04315"
     CDS             <1..652
                     /locus_tag="VIH70_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115618.1"
                     /translation="LFGAALVRRYAGSRRPYELVWALALLMYAGASLAVAFGVANGWT
                     SGEFRVYWVLGAVLNVPFLAAGELELLFHGRGVRWTLYVLLAFITAYTVAVVRTAEIH
                     AAALAQDLPSGKEVFGDGTAAHRLPQIVAIPAYLVLLGGTLWSARRMRGRPELRDRFW
                     GTLVIAFGATIIAGFGSAFAALGHLVPFSLSLLVGICVMFLGFLRAARRRDLAPTA"
     gene            complement(690..1433)
                     /locus_tag="VIH70_04320"
     CDS             complement(690..1433)
                     /locus_tag="VIH70_04320"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8115619.1"
                     /translation="MTEDHERIDELLAGYVLLSLSGEEAAEADRILVDHVPTCATCRA
                     TLSEFQAVSGDLALAAPPVDPPETLLPRLHHSMDEVPLAGRTPRRGALIAVAASAVAL
                     VAMGGLSLVLGNRLSNAETRTGTALEILSAMRAPGASPVNVSPQDQTPPGSGMVEVSA
                     PDIRRLYIAAEFCPEPAPGHAYQLWLGSNGEWSPVGDMFWPDDGVLLLEVEGVDVSQY
                     DEIWITQEPVTQPPTRPSTDGPSWFGSLT"
     gene            complement(1438..2001)
                     /locus_tag="VIH70_04325"
     CDS             complement(1438..2001)
                     /locus_tag="VIH70_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8115620.1"
                     /translation="MDEDDRHDLELHRLVSGRERSAFDELYRRYGRSAYGVAIRVTSQ
                     DLIAQEVVQDAFLALWRAPEAFDPARGAFRSFFLSLVHHRAVDAVRREERLRRRTERA
                     ANLEPATGEDPAEDVVEDAYLRVRRKEVRAALATLPPAQQQVLEMAYFEAKTQKRIAE
                     ELGIPIGTVKTRTLAAMRKLREALREE"
     gene            2062..2298
                     /locus_tag="VIH70_04330"
     CDS             2062..2298
                     /locus_tag="VIH70_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY8115621.1"
                     /translation="MPEVLMYARPGCGLCDEAREVIMAVRARTPFEYREVDVTGDDPL
                     ELEYGIRIPVVLVDGRELFEIRVDAGRLAEVVRT"
     gene            2344..3045
                     /locus_tag="VIH70_04335"
     CDS             2344..3045
                     /locus_tag="VIH70_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEY8115622.1"
                     /translation="MTERSIPEATVGRLPVYLRALVELAESGSSTVSSEALAELTGVN
                     SAKVRKDLSHLGSYGTRGVGYDVAYLVHQIRRELGLTQHWPIVIAGIGNLGHALANYR
                     GFAERGFRPAALVDNDPAKVGEEVAGLRIRPVEDLPAIAGEHDALIGVICTPAPSAQA
                     VADAMVRAGVRSILNFAPVALVVPEGVSVRKVDLAVELQILTYYEQRKAALAEVKGRQ
                     GRGRRLVGRPATSRS"
     gene            complement(3149..3892)
                     /locus_tag="VIH70_04340"
     CDS             complement(3149..3892)
                     /locus_tag="VIH70_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115623.1"
                     /translation="MLGVGCLGFEIGCETMLKGGLAVVMAFVLFVGSVLIVLAAVFGR
                     RMGYLVLAVSFFGWMILLSSLWTFGFYSQGLDTPVNLGPRGAEPAWVVEAAGTDPEPV
                     YEAFASYPGDGWREPGTNDADTSSVQSVTAAAQGFLAEQANEDLGKSEHEPGALTTTD
                     FTVQNVEFYPTDDGVSLSAAHAFFNDGGPILTVYLRHDSGSVNRYSWMFLIGSTLLFG
                     AHLPFLDRAERKRKEILTGGTAPPWYGPA"
     gene            complement(3892..4641)
                     /locus_tag="VIH70_04345"
     CDS             complement(3892..4641)
                     /locus_tag="VIH70_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8115624.1"
                     /translation="MLGLSQAQGIVLAVGGAIFLAIAGGVLILRGRQRREAEIPNAMR
                     PGPSDPDLETPLLHKLQGWGVVLVLFFVIWVPAVWLLEPEQNLDQDQDLISDAIARGA
                     HAVQIYTEDNQAGVGCVQCHGPTLQGSLIPNTQTPDDPYDVQPTPNLTTVCGGPFTGH
                     PLIYGLNDIYTTIEQGRGLMPSWSIRFAGALGDQQINDIVNYIVSIQGEDEVKFSENV
                     CTNPKAVTKAIDQFLDGDPTKKPNPTTNIEF"
     gene            complement(4641..5378)
                     /locus_tag="VIH70_04350"
     CDS             complement(4641..5378)
                     /locus_tag="VIH70_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8115625.1"
                     /translation="MTPLLALSSGTVAVIVVIVVIDLFAIFFGLSFARSRKAQREANA
                     AAGAAILGEPAPPAPKPVSRREFFRRSLVASLLVFGAEFGGATIAFLWPNLRGGFGSV
                     INAGKLDDILAYIEDNDLPFYSGIGRFYIVKYNGKPEGDVDYATEGVTAQGIMPLYQR
                     CVHLGCRVPFCQQSQWFECPCHGSKYNTAGEYKLGPAPHGMDRFKVTVTDTGDVMVDT
                     AEVVLGPPRGTDTIHKPLAGPFCVAVQ"
     gene            complement(5375..6271)
                     /locus_tag="VIH70_04355"
     CDS             complement(5375..6271)
                     /locus_tag="VIH70_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEY8115626.1"
                     /translation="MARDDAALDQETFDRVLAEELGKGTDRRVAEGRARAAALRAARA
                     KSGEGPAATPAPAPAAGDGGAPEPAAAPAAAAGGPAAAAPAAAVAAPAAVGATAPAAA
                     APASVAAATLTAPERQAPAPTGNVPEPKKGAPEKHRLLALVPPEGIQRVEREQGDRIN
                     VWPHLLIEEFVAMMIVLAALTIFATFVNAPLRELANPNLTPNPSKAPWYFLGLQELLR
                     YFHPMVAGVLFAPTLVLLALAVAPYVDRNPSTKPGDRKIAITLFTMLLMFGAVLTIIG
                     SFFRGTGYNWVWPWAQGVFFEL"
     gene            complement(6286..7059)
                     /locus_tag="VIH70_04360"
     CDS             complement(6286..7059)
                     /locus_tag="VIH70_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY8115627.1"
                     /translation="MAKLKLPTPTEALDRVRETEVWRSVFRPGSIFRRGYRDTSRDRA
                     LATMNNVLYHLHPVKMKRHGLKLTYTFCLGGLSFFLFILLTITGIYLMFFYRPAAELG
                     SAAAYTDMQNIRTSVFFGDLVRNLHRWGAHLMVFTVTLHMARVFYTGSYKPPREFNWV
                     VGVLLLFLTLGLSFTGYLLPWDQLAVWAVTVGTSLAGYSPLIAKQANFLLLGGVIVGP
                     DTLLRWYVLHVLALPFVLVIFLSVHFWRIRKDGGISGPL"
     gene            complement(7087..7485)
                     /locus_tag="VIH70_04365"
     CDS             complement(7087..7485)
                     /locus_tag="VIH70_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY8115628.1"
                     /translation="MPFTKTEVDVHTDYVVETVDPDWLAKGVKSPKHLMLDPDNCILC
                     RACEDVCPWNCIYMMSTDIVQDAEDDAVGAEVRTAYSVFVVDDNACTRCSVCVDRCPT
                     DTLYYARLPEAPRGQRTMASVPESQRAEAG"
     gene            complement(7498..7848)
                     /locus_tag="VIH70_04370"
     CDS             complement(7498..7848)
                     /locus_tag="VIH70_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115629.1"
                     /translation="MKVPKVLVKAKERAEPIVKNFEWTWTTAVLFSLGFAFFSVITMA
                     VIPSFWLYFAEQKLGWGGLSGTSDWHPLARDFVAANLAVGPFVTVVVIGAIMQNWRRK
                     LRGGTSDRPTGGYR"
BASE COUNT         1231 a         2749 c         2867 g         1243 t
ORIGIN      
        1 gctgttcggc gcggcgctcg tgcgccggta cgccggcagc cgtcggccgt acgagctggt
       61 ctgggcgctc gcgctcctga tgtacgcggg ggcgtccctc gccgtcgcgt tcggcgtcgc
      121 gaacggatgg acctcggggg agttccgcgt ctactgggtg ctcggcgccg tgctgaacgt
      181 cccgttcctc gcggcgggtg agctcgaact gctgttccac ggccgcggcg tccgatggac
      241 gctgtacgtg ctgctcgcgt tcatcacggc ctacaccgtg gcggtcgttc gcaccgcgga
      301 gatccacgcg gcggccctcg cgcaggacct gccctcgggc aaggaggtct tcggcgacgg
      361 caccgcggcc caccggctgc cgcagatcgt cgcgatcccc gcgtacctcg tcctgctcgg
      421 cgggaccctg tggtcggccc ggcgcatgcg cgggcgaccc gagctccggg accggttctg
      481 gggcacactc gtgatcgcgt tcggcgcgac gatcatcgcg gggttcgggt ccgcgttcgc
      541 cgcgctcggc catctcgtcc cgttctcgct ctcgctcctc gtgggcatct gcgtgatgtt
      601 cctcgggttc ctgcgcgcgg cgcggcgacg cgatctcgca ccgacggcct gacgcatcca
      661 gcgtcgaggt gatcggcccg gcagttccgt cacgtgagcg agccgaacca gctgggtccg
      721 tcggtgctcg gccgcgtcgg aggctgggtg accggctcct gcgtgatcca gatctcgtcg
      781 tactgggaga cgtccacccc ctcgacctcg agcaacagga cgccgtcgtc cggccagaac
      841 atgtcgccca ccggcgacca ctcgccgttg ctcccgagcc acagctggta cgcgtgaccg
      901 ggggcgggct cgggacagaa ctccgcggcg atgtacaggc ggcggatgtc gggcgcggag
      961 acctcgacca tcccactgcc cggcggcgtc tgatcctgcg gcgacacgtt caccggcgaa
     1021 gcgcccggcg cccgcatcgc cgagaggatc tcgagcgcgg tgcccgtgcg cgtctcggcg
     1081 ttcgagaggc ggttccccag cacgagcgag aggccaccca tcgcgaccag cgcgacggcc
     1141 gaggccgcga ccgcgatcag cgcgccgcga cgaggcgttc ggccggcgag cgggacctcg
     1201 tccatcgagt gatgaaggcg gggcaggagg gtctcgggcg gatcgacggg cggcgcggcg
     1261 agcgcgagat cgcccgacac ggcctggaac tcggagaggg tggcccggca cgtcgcgcac
     1321 gtgggcacgt ggtcgacgag gatccggtcg gcctcggccg cctcctcgcc cgagagcgag
     1381 agcagcacgt agcccgccag gagctcgtcg atgcgctcgt gatcctcggt cacgacgtca
     1441 ctcctctcgc agggcttcgc gcagtttccg catggccgcc agcgtcctcg tcttcacggt
     1501 tccgatcggg atgccgagct cctcggcgat ccgcttctgc gtcttggcct cgaaatacgc
     1561 catctcgagc acctgctgct gcgccggagg aagggtcgcg agcgcggcgc gaacctcctt
     1621 gcgccgaacg cgcaggtagg cgtcctcgac gacgtcctcc gcagggtctt cgccggtcgc
     1681 gggctcgagg ttcgccgctc gttcggtccg gcgcctgagg cgctcctcac gccggacggc
     1741 atcgacggcc cggtggtgga ccagggacag gaagaaggag cggaacgccc cccgcgcggg
     1801 gtcgaacgcc tcgggcgccc gccacagcgc caggaaggcg tcctggacga cctcctgggc
     1861 gatgaggtcc tgcgacgtca cgcggatcgc cacgccgtag gccgaccggc cgtaccgccg
     1921 gtacagctcg tcgaaggccg accgctcgcg acccgacacc agccggtgca gctcgagatc
     1981 gtgacgatcg tcctcgtcca cgggctgttt cctaccacac gcgggccgct cctcccttcc
     2041 tggcctcgtt agcatgcggg cgtgcccgag gtcctgatgt acgcgcgccc gggttgcggc
     2101 ctgtgcgacg aggcccgcga ggtgatcatg gccgtgcggg cgcggacgcc gttcgaatac
     2161 cgcgaggtcg acgtcaccgg cgacgacccg ctcgagctcg agtacgggat caggatcccc
     2221 gtcgtgctcg tggatgggcg cgagctcttc gagatccgcg tcgacgcggg tcgcctggcg
     2281 gaggtcgtgc ggacttagtg cattcgtgaa ggggcgttca cgaatgcact atcatcctcc
     2341 gccgtgaccg agcgatcgat cccggaggcg acggtgggac ggctcccggt ctacctccgc
     2401 gccctggtcg agctcgcgga gtccgggagc tcgaccgtgt cctcggaggc gctcgccgag
     2461 ctcacggggg tgaactcggc gaaggtgcgc aaggacctgt cgcatctcgg ctcgtatggc
     2521 accagggggg tggggtacga cgtcgcctac ctcgtgcatc agatccgccg cgagctcggg
     2581 ctgacccagc actggccgat cgtgatcgcg gggatcggga acctcgggca cgcgctcgcg
     2641 aactatcggg ggttcgccga gcgcgggttc cggcccgcgg cgctcgtcga caacgacccg
     2701 gcgaaggtcg gggaagaggt cgccgggctc cggatccggc cggtcgagga cctgccggcg
     2761 atcgcgggcg aacacgacgc gttgatcggc gtgatctgca cgcccgcacc gtcggcgcag
     2821 gccgtcgccg acgcgatggt ccgcgcgggg gtgcggtcga tcctgaactt cgcgccggtg
     2881 gccctggtcg tccccgaggg ggtctcggtg cgcaaggtgg acctggcggt cgagctccag
     2941 atcctgacct actacgagca gcgcaaggcc gcgctcgcgg aggtcaaggg gcgtcagggc
     3001 cgcggccgac gcctggtggg tcggccggct acttcgcgat cgtgaaccag agcgcgatga
     3061 agatcacgat cacggtcacg ccgaacagga tgaactgcac ggctccctgc tcgatcagcg
     3121 gcgcttcttg ggccagcatg aattcctcct aggcgggtcc gtaccacggc ggggccgtcc
     3181 cgcccgtcag gatctccttg cgcttgcgct ccgcccggtc caggaagggc aggtgggcgc
     3241 cgaacaacag cgtcgaaccg atcaggaaca tccaggagta ccggttgacg ctgccggagt
     3301 cgtggcggag atagaccgtc aggatcgggc cgccgtcgtt gaagaacgcg tgcgcggccg
     3361 agagcgagac cccgtcgtcg gtcgggtaga actcgacgtt ctggaccgtg aagtccgtgg
     3421 tcgtgagggc gccgggctcg tgctcgctct tgccgaggtc ctcgttcgcc tgctcggcga
     3481 ggaagccctg cgccgccgcc gtcaccgact ggaccgagga cgtgtccgcg tcgttcgtcc
     3541 ccggttcgcg ccaaccgtcg cccgggtagg acgcgaaggc ctcgtatacc ggctcgggat
     3601 cggtcccggc cgcctcgacg acccacgcgg gctcggcgcc gcgggggccg aggttcacgg
     3661 gcgtatcgag cccctgcgag tagaagccga acgtccacag cgaggacagc aggatcatcc
     3721 agccgaagaa cgagaccgcg agcaccaggt accccatccg ccggccgaac accgccgcga
     3781 gcacgatcag cacgctgccc acgaagagca cgaacgccat gaccacggcg agcccgccct
     3841 tcagcatcgt ctcgcacccg atctcgaacc cgaggcagcc gacgccgagc atcagaactc
     3901 gatgttggtc gtcggattgg gtttcttggt ggggtcgccg tcgaggaact gatcgatcgc
     3961 cttggtcacg gccttggggt tcgtgcacac gttctcgctg aacttcacct cgtcctcgcc
     4021 ctggatgctg acgatgtagt tcacgatgtc gttgatctgc tgatcgccga gcgcgcccgc
     4081 gaaccggatg ctccacgacg gcatcagacc tcggccctgc tcgatcgtcg tgtagatgtc
     4141 gttcaaaccg tagatcagcg ggtgcccggt gaacggcccg ccgcagaccg tcgtcaggtt
     4201 cggcgtcggt tggacgtcgt aggggtcgtc cggggtctgt gtgttcggga tgagggaccc
     4261 ctgcagggtc gggccgtggc actgcacgca gcccacgccg gcctggttgt cctccgtgta
     4321 gatctggacg gcgtgcgcgc cgcgggcgat cgcgtcgctg atcaggtcct ggtcctggtc
     4381 gaggttctgc tcgggttcga ggagccagac ggccggcacc cagatcacga agaacaggac
     4441 aagcaccacg ccccatccct gcagcttgtg gagcagcggg gtctcgaggt cgggatcgga
     4501 gggacccggg cgcatcgcgt tcgggatctc ggcttcccgc cgctggcggc ctcgaaggat
     4561 gagcacgccg ccggcgatcg cgaggaagat cgcgccgccg accgcaagga cgatgccctg
     4621 ggcctgcgac agcccgagca tcactgcacc gccacgcaga acggcccggc caggggtttg
     4681 tggatcgtgt cggttccccg gggtgggccg aggacgacct cggcggtgtc gaccatgacg
     4741 tcgccggtgt cggtgaccgt gaccttgaac cggtccatcc cgtgcggggc cgggccgagc
     4801 ttgtactcgc cggcggtgtt gtacttcgag ccgtggcacg ggcactcgaa ccactgcgat
     4861 tgctggcaga acggcacccg gcaccccagg tgcacgcacc gctggtagag cggcatgatc
     4921 ccctgcgccg tgacgccctc ggtcgcgtag tcgacgtcgc cctcgggctt gccgttgtac
     4981 ttgacgatgt agaaacggcc gatgcccgag tagaagggaa ggtcgttgtc ttcgatgtag
     5041 gcgaggatgt cgtcgagctt gccggcgttg atcaccgagc cgaagccgcc ccgcaggttc
     5101 ggccacagga acgcgatcgt cgcgcccccg aactccgcgc cgaacaccaa cagcgatgcg
     5161 acgagcgagc ggcggaagaa ctcccgacgg ctcaccggct tgggcgcggg gggcgccggt
     5221 tcgcccagga tcgcggcacc ggccgcggcg ttcgcctccc gctgggcctt cctggaccgg
     5281 gcgaacgaca gcccgaagaa gatggcgaac agatcgatga ccacgatcac cacgatgacg
     5341 gcgacggtgc cgctcgagag ggcgaggagg ggggtcatag ctcgaagaag accccctggg
     5401 cccacggcca gacccagttg tagccggtgc cgcggaagaa cgagccgatg atggtcagca
     5461 ccgccccgaa catcaggagc atcgtgaaca gcgtgatcgc gatcttgcga tcgcccggct
     5521 tggtgctggg gttccggtcc acgtagggcg cgaccgccag ggcgagcagc acgagcgtcg
     5581 gcgcgaacag cacgcccgcg accatcgggt ggaagtagcg gagcagctcc tggaggccga
     5641 ggaagtacca cggcgccttc gacgggttcg gggtcaggtt cgggttcgcg agctcgcgga
     5701 ggggggcgtt cacgaacgtc gcgaagatcg tgagcgccgc gaggacgatc atcatcgcca
     5761 cgaactcctc gatcagcagg tgcggccaca cgttgatgcg gtcgccctgc tcgcgttcga
     5821 cgcgctggat cccctcgggc gggacgagcg ccagcagccg atgcttctcg ggcgcaccct
     5881 tcttcggctc ggggacgttg ccggtgggcg cgggcgcctg gcgctcgggc gcggtgagcg
     5941 tcgcggccgc gacggatgca ggggcggcag ccgccggcgc cgtggcacca acggcggcgg
     6001 gtgcggccac ggcagcggcg ggcgcggcag cggcggggcc cccagcagct gcggcgggcg
     6061 cggcggcggg ttcgggtgcc cctccatcgc cggcggccgg cgcgggcgcc ggtgtcgcgg
     6121 cgggcccctc cccggacttc gcgcgcgccg cccggagggc ggcggcccgc gcgcggccct
     6181 cggccacgcg tcggtcggta cccttgccga gctcctccgc caggacccgg tcgaaggtct
     6241 cctggtcgag cgctgcgtcg tcccgagcca tcgtcccctt ctcagctaga gcggtcccga
     6301 gatgccgccg tccttgcgga tccgccagaa gtggaccgag aggaagatca ccaggacgaa
     6361 cggcagagcg agcacgtgca agacgtacca ccggagcaag gtgtccggac ccacgatcac
     6421 cccaccgagc agcaggaagt tggcctgctt ggcgatcagc ggcgagtagc cggcgagcga
     6481 ggtcccgacc gtgaccgccc acaccgccag ctggtcccac ggcaacaggt agccggtgaa
     6541 cgacagtccc agcgtgagga acagcagcag cacgcccacg acccagttga actcgcgggg
     6601 cggcttgtac gagccggtgt agaagaccct ggccatgtgc agggtcacgg tgaacaccat
     6661 caggtgcgcg ccccatcggt gcaggttgcg gaccaggtcg ccgaagaaca ccgacgtgcg
     6721 gatgttctgc atgtcggtgt aggccgcggc cgagccgagc tcggcggcgg ggcgatagaa
     6781 gaacatcagg tagatgcccg tgatcgtcag aaggatgaac aggaagaacg agaggccacc
     6841 caggcagaac gtgtaggtga gcttcagccc gtgacgcttc atcttcaccg ggtgcaggtg
     6901 atacagcacg ttgttcatcg tcgcgagagc gcggtcgcgc gaggtgtctc ggtagccgcg
     6961 acggaagatc gaaccggggc ggaagaccga tctccagact tcggtctcgc ggacgcggtc
     7021 gagggcttcg gtgggcgtcg gcagcttcag cttcgccacg tcgtccccct catcccttcc
     7081 cccggcctag ccggcctcgg cgcgttgcga ctcgggcacc gaggccatcg tgcgctgccc
     7141 gcgcggggcc tccggcagcc gcgcgtagta cagcgtgtcg gtggggcacc ggtcgacgca
     7201 caccgagcag cgcgtgcacg cgttgtcgtc gacgacgaac accgagtacg cggtgcggac
     7261 ctcggccccg acggcgtcat cctccgcgtc ctggacgatg tcggtcgaca tcatgtagat
     7321 gcagttccac gggcagacgt cttcgcacgc tctgcacagg atgcagttgt cggggtcgag
     7381 catcaggtgc ttgggcgact tgacgccttt cgcgagccag tcggggtcga cggtctcgac
     7441 gacgtagtcg gtgtggacgt cgacctcggt cttggtgaac ggcattgggc taccccctca
     7501 ccggtagcca ccggtgggtc ggtcggaagt gccgccccgg agcttgcggc gccagttctg
     7561 catgatggcc ccgatcacga ccacggtcac gaacggacca acggcgaggt tcgcggcgac
     7621 gaagtcccgg gcgagcgggt gccagtctga ggttccggac aggcctcccc agccgagctt
     7681 ctgctccgcg aagtacagcc agaacgaggg gatgaccgcc atcgtgatga cgctgaagaa
     7741 ggcgaacccc agggagaaca ggaccgcggt cgtccaggtc cattcgaagt tcttgacgat
     7801 cggctccgcg cgttccttcg ccttgacgag caccttcggg accttcattc ggcctccgtc
     7861 gccacgaccg tcctctcgtt ccccacgtct tcgtgcgctg ggcctcgttc gtgcctgaac
     7921 cgggtcggat tatagtcacg cgctcccacg gtccccgcac cgtcgctgac cgcgctcagt
     7981 gcttgtgaat cggttcacaa ggctatcggt cactatcgca ggagcggacg ctcaaccgca
     8041 acccactgcc cgataggcga cgggccccct gcgcgagcgc cgaccctaga
//
LOCUS       DATBRK010000143         3400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_21145, whole genome shotgun sequence.
ACCESSION   DATBRK010000143 DATBRK010000000
VERSION     DATBRK010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21145"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VIH70_04375"
     CDS             <1..337
                     /locus_tag="VIH70_04375"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEY8115630.1"
                     /translation="SADAIIGLGVYAPGGLLAAAARLASRGPWFNLVVSNIPGPPQPM
                     YMAGARLVAQYPSLPLGENSALSIACTSIGGTMAFGLTGDYDGMPDLQRLAFALEESL
                     GEVSKAAGL"
     gene            complement(334..1227)
                     /locus_tag="VIH70_04380"
     CDS             complement(334..1227)
                     /locus_tag="VIH70_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8115631.1"
                     /translation="MQIELPPSRFADLDGPLHYVEWNGPPGRTFVLVHGLGGSLLSWL
                     AVAPGLARHGRVLAMDLPGFGRTERNGRRSRVSDLRGTVSRFIEEVAGGGPVVLVGNS
                     MGGGISMLEAALEPAAVEALVLSNSVYPWKRGSSLPAPIVMVGFALYELPRVGEWASR
                     QRVTRLEAQRAVELGLRIIAADPSTIPDELVRLHVEQLVRHQQNPDAGPAFLEAARSL
                     LALGRRRDVVGWIIGAVKPPVLVVHGREDRLVPLGFAMGALELRPDWDMRLIPKVGHV
                     PQMEAPERWLAAVEPWLEGLG"
     gene            1271..2611
                     /locus_tag="VIH70_04385"
     CDS             1271..2611
                     /locus_tag="VIH70_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY8115632.1"
                     /translation="MAIVRLRPPLRELAEGRREVIVQGGTVVDVLRELERSHPRLDGW
                     ILDDRGRVRQHVAVFLGGERVDGDARVASEERLDVIGAVSGGAVSGGAATELLVGTKK
                     GLFVLRGDREREMDVVARAFPGSSVEFAIRDPRTGRYFASVTHGQFGPRVYLTDDPTG
                     AWEQAEGPRFPEETDTTLVRTWVITPGERDGVLYAGVDPAALFESRDDGVTWELNRPL
                     WDQPSRPDWGPGAGGLCLHSICPWPGEPDRLAVGISSVGVWVTEDGGASWEIGNAGIV
                     PRYLPEEARSTEPLARCVHNMHRAPLRPERLFMQFHGGVYRSDDAARSWTSIAGGLPS
                     DFGFPVVIDPNDPEAAYVIPLNSDLDRVSAQGRLRVFETRDAGATWTGRTEGLPQDDA
                     YLTILRQAFARDDGSPLGLWFGATSGDVFGSSDAGATWRVVARGLAPVLSVRAA"
     gene            complement(2660..2917)
                     /locus_tag="VIH70_04390"
     CDS             complement(2660..2917)
                     /locus_tag="VIH70_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115633.1"
                     /translation="MDRDTLQDDLDLMLEPSRGDELPGEWDWEPPLGTGAPVASLAAE
                     ARETEEMGTEASSEGASDPPDGPGDETEDEAEDDAEEEEDD"
     gene            3242..>3400
                     /locus_tag="VIH70_04395"
     CDS             3242..>3400
                     /locus_tag="VIH70_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HEY8115634.1"
                     /translation="MPEPARPLGTDDADDLRLYLEAAAREPLLTKEEEVELAILIEHG
                     KEGEERLRA"
BASE COUNT          460 a         1210 c         1219 g          511 t
ORIGIN      
        1 gagcgccgac gcgatcatcg gcctcggcgt ctacgcgccc ggcggcctgc tcgccgccgc
       61 cgcccgcctg gcgagccggg gtccctggtt caacctcgtc gtttcgaaca tccccgggcc
      121 gccgcaaccg atgtacatgg caggcgcgcg gctggtcgcg cagtacccct cgctcccgct
      181 cggcgagaac tcggcgttgt cgatcgcgtg tacctcgatc ggcgggacga tggcgttcgg
      241 cctgaccggc gactacgacg gcatgccgga cctgcagcgg ctcgcgtttg cgctcgagga
      301 atcgctcggc gaggtctcga aggccgccgg cctctagccc aggccttcga gccagggttc
      361 gacggcggcg agccagcgtt cgggcgcctc catctgcggc acatggccca ccttcgggat
      421 cagccgcatg tcccagtccg gacggagctc gagcgccccc atggcgaacc cgagcgggac
      481 gagccggtcc tctcgcccgt ggacgacgag caccggaggc ttcaccgcac cgatgatcca
      541 gccgacgacg tctcgccggc ggccgagggc cagcagcgac cgggccgcct cgaggaacgc
      601 cggcccggcg tcggggtttt gctggtggcg gacgagctgc tccacgtgga ggcggacgag
      661 ctcgtcgggg atcgtcgagg ggtctgcggc gatgatgcgc aggcccagtt cgaccgcgcg
      721 ctgggcctcc aggcgcgtga cgcgttgccg tgaggcccac tcgccgaccc gggggagctc
      781 gtacagcgcg aacccgacca tcacgatcgg cgccggcagc gacgagccgc gcttccacgg
      841 gtagaccgag ttcgagagca cgagcgcctc gaccgccgcg ggctcgagcg cggcctcgag
      901 catcgagatc ccgcctccca tcgagttccc cacgagcacg accggtcctc cgcccgcgac
      961 ctcctcgatg aacctggaca cggtgccacg gaggtcgctc acgcggctgc ggcggccatt
     1021 gcgctcggtc cggccgaacc ccggcaggtc catcgccagc acccgcccgt gcctcgcgag
     1081 acccggcgcc accgcgagcc acgacagcag cgaaccgccc aggccgtgga cgagcacgaa
     1141 cgtgcgcccc ggcggcccgt tccactcgac gtagtgcagg ggaccgtcca ggtccgcgaa
     1201 ccggctcggc ggcagctcga tctgcatggg cgttcggcga cctccgcccg ccattctcta
     1261 cactcggggc atggcgatcg tccgactgcg gcccccgctc cgcgagctgg ccgagggacg
     1321 tcgcgaggtg atcgtccagg gcggcaccgt ggtcgacgtg ctgcgggagc tcgaacggtc
     1381 gcacccgagg ctcgacggct ggatcctcga cgaccgaggg cgggtccgtc agcacgtcgc
     1441 cgtgttcctc gggggcgaac gggtcgacgg cgacgcccgc gtcgcgagcg aggaacgcct
     1501 cgatgtgatc ggggccgtct ccggcggggc cgtctccggc ggggcagcga cggagctcct
     1561 ggtcgggacg aagaagggtc tgttcgtgct acgcggcgat cgcgagcgcg agatggacgt
     1621 cgtggcccgg gcgttcccgg gaagctcggt cgagttcgcg atccgggacc cccggacggg
     1681 ccggtacttc gcgagcgtca cccacgggca gttcgggccg cgggtctacc tgacggacga
     1741 cccgaccggg gcctgggagc aggcggaggg tccccggttc ccggaggaga cggacacgac
     1801 cctggtccgc acgtgggtga tcacgccggg tgagcgggac ggcgtcctgt acgccggtgt
     1861 cgaccccgcc gcgctgttcg agagccgcga cgacggggtc acgtgggagc tcaaccgtcc
     1921 gctctgggat cagccgagcc ggccggactg ggggccggga gccggcggcc tctgcctgca
     1981 ctcgatctgt ccgtggccgg gcgagcccga tcgtctcgca gtcggcatct cctcggtcgg
     2041 cgtgtgggtc accgaggacg ggggagcatc gtgggagatc ggcaacgcgg gcatcgtccc
     2101 gcggtacctg ccggaggagg cgcgctccac cgaaccgctc gcccgctgcg tgcacaacat
     2161 gcaccgggcg cctctccgac ccgaacgcct cttcatgcag ttccacggcg gcgtctaccg
     2221 ctccgatgac gccgcgcgct cgtggaccag catcgccggc ggtctgccga gcgatttcgg
     2281 gttcccggtg gtcatcgatc cgaacgaccc agaggcggcc tacgtgatcc cgctgaactc
     2341 ggacctcgac cgggtctcgg cgcagggccg gctccgggtg ttcgagacgc gcgacgccgg
     2401 cgcgacgtgg acgggtcgga ccgaggggct cccacaggac gacgcgtatc tcacgatcct
     2461 tcgtcaggcg ttcgcccgcg acgacggaag cccgttgggt ctatggttcg gcgccacgtc
     2521 gggcgacgtg ttcggttcct cggatgccgg tgcgacctgg cgggtggtgg ctcggggcct
     2581 ggcgcccgtc ctgtcggttc gcgcggcgta acgaagggcc cgaggggctg gcgaccgccc
     2641 cttcccgccg ggggaacgct cagtcgtcct cttcttcctc ggcgtcgtcc tctgcctcgt
     2701 cttctgtctc gtctcctggc ccgtccggcg ggtcgctcgc tccctcgctg gatgcctcgg
     2761 ttcccatctc ctcggtctcc ctcgcctccg cggccaggct cgcgaccggg gcgcccgtcc
     2821 cgaggggggg ctcccagtcc cactcgccgg ggagctcgtc gccacggctc ggttcgagca
     2881 tcaggtccag gtcgtcctgc agcgtgtcgc gatccatgcc gcctcctctc gcccgaattc
     2941 gctcatcgaa cttgagtcgg gtggcgggag cgcatctcgg ccgagaccgc catgtgaatg
     3001 tcttgtggcg ttccgtttcc cgacgcttcg acaggcacgg atcggggaat aacgtgcatc
     3061 tccacggggt ctccacaggt gtcggcttta cagaggctca ggggtggggc tagcatggtc
     3121 cctggtggcc cggggaggcc ggtcttcgta tgcccggggc ccaatcgact gagatcggtc
     3181 ccgaagacct caaggggcga agcacgctcc gtacggaggc ttcgaggagg agagtgagtt
     3241 catgccagaa cctgcacgtc cgctcggcac ggatgacgcc gacgacctcc ggctgtacct
     3301 ggaggcggcg gcgcgcgagc cactcctcac caaggaggag gaggtcgagc tcgccatcct
     3361 gatcgagcat ggcaaggagg gggaggagcg cctgcgcgcc
//
LOCUS       DATBRK010000144         6532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1031445, whole genome shotgun sequence.
ACCESSION   DATBRK010000144 DATBRK010000000
VERSION     DATBRK010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031445"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VIH70_04400"
     CDS             complement(<1..309)
                     /locus_tag="VIH70_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HEY8115635.1"
                     /translation="MTTVLLFHHARGQTPGFLEFVDELREAGHKIHAPDLYEGKTFGT
                     VDEGVANAKRVGFSEIIRRGSEAAQGLPADIVYAGFSLGVMPAQSLTQTRQGARGAIL
                     F"
     gene            733..1971
                     /locus_tag="VIH70_04405"
     CDS             733..1971
                     /locus_tag="VIH70_04405"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY8115636.1"
                     /translation="MPNAVRRIGLGTVFALLTGTLVLGHALKAPCASGEWGDNRQYTR
                     LCYSDNVHQLGTEQLNGGRLPFLDACADTTAECDEYPLLTMWTMRVAAWISGPSTTGY
                     FYASAIMLWIAAFVIAFCLYLLVGDRVLYFALAPTLALYATMNWDLLAVALATVGTLA
                     YLRRSDGWSGALLGLGAAAKIFPMFLVVPFVAARFRGEEPDRGIRLAWAAVGTWVAVN
                     LPFVLLAPTGWWEFFRYNAARLADWDSLWYLACQRTTGETCTNTRLVNLASVGLFVAW
                     VAVVWILKERREPGFPRWTLGFPILVLFLLTNKVYSPQFSLWLLPWFALAFPRLGWFA
                     AFELADVAVFVTRFSWFGRYLGIDGGVAGLPVGVFELAVAIRALILVACVIGWVRGAR
                     AGPRPAAPDEERPVMAGVAA"
     gene            2046..3287
                     /locus_tag="VIH70_04410"
     CDS             2046..3287
                     /locus_tag="VIH70_04410"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY8115637.1"
                     /translation="MSPSRRTIGIGAVFLLLAGTLAIGHAIKTPCVHGDWSDGRPFTW
                     LCHTDIIPLLGNEQLYGDRLPYLEPCEQTEGTCDEYPVLTIWMMRLTASVARPHNTRF
                     FYANAILLWLAALWTAYLLYTVVGRRALYFALAPTLAIYGTLNLDLLPVALATGGTVA
                     YLRRRDVTSGVLLGLGAAAKLYPALLVIPFVVGRFRGREPDRGIYLVWAAVGAWIAVN
                     LPFALAGTSGWLEFFRYNAFRPADWDSLWFIACEQATGMGCTNTRLVNLGSLVLFLGW
                     LAIVWTSKGRRRPGFARWTLGFPILVIFLLTNKVYSPQYSVWLLPWFALALPHLRLFL
                     AFEAADTAVFVTRFSWFGLLGGHHNGFAGSPLGAFEAAILVRAAILVVCVIAWIRAGE
                     PISRLERTPPDRTDARVEVPV"
     gene            3284..4489
                     /locus_tag="VIH70_04415"
     CDS             3284..4489
                     /locus_tag="VIH70_04415"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEY8115638.1"
                     /translation="MTTGTASLIELSDGHDGWRERVVRALRFCLFAFVALRVAMFVIG
                     VVGVTLFPPLGAVGVPGWPAPAPNPGWQNAFTAWERFDALWFLRIADGGYRLRDGSAA
                     FFPLYPLAIRGLSLALGGHPLAAATIVSNAAFFGGLVVTYLLTSSELSERAARTTVLL
                     MCVFPTAHFFLMPYSESLFFLLSVTAFWGARRRRWAIAGAAGALAALTRSIGIVIAPA
                     LVIEALHQRIERRGPAWPGFLAAAATGGGLLAYLGSWGLRAGEWLAPVTFQANWQRSL
                     SWPWATLWDGTGDAFRYLDDLNGGYWLIDWLIVIPMLAASVYALVRLRPAYGVYLWSA
                     LLIPLTYVFPDRPLMSMPRFVLPLFPAFWGLALGLERLRIPRSAALGVSAAGLGILSV
                     LTVNWYYIF"
     gene            4546..5184
                     /locus_tag="VIH70_04420"
     CDS             4546..5184
                     /locus_tag="VIH70_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8115639.1"
                     /translation="MTIRVAIADDHSLVRQGLRRYLDMAEGIEVVGEAANGQELVDLV
                     AKEEPDIALVDIRMPEMDGLEAARKIRETHPKTGVIMLTAYDDRQFVVEAVRSGARGY
                     VLKARDAEHLIQTVRLVAGGNMVIDPQLVVALAEELSQAKERDRRAETLTAREIEVLQ
                     LLAFGHTNRDIAERLYISPDTVKTHLEHIFEKLGASDRTAAVAEALRRRLIE"
     gene            5339..>6532
                     /locus_tag="VIH70_04425"
     CDS             5339..>6532
                     /locus_tag="VIH70_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8115640.1"
                     /translation="MSTQTQRRAPWFAPFVLLVAGGGVAFAYAASSRSGWPDDIRSPL
                     VAGSCLLSAIVILLRCRHKGAWRSAPALGLGIAAIAVVSAGLLVTDATGSEPPRLGLY
                     DVGFLAAGVLFLIAVAIEFREHVAREDRREIVADVALLTAAIGTMLFLSLRPDSMADP
                     ARSAEFALIIASGASAYGALALARPNVAHLGMFFAVCGMCVGVHGFAKLWLSGSFEVG
                     RPQVDLPIAFATLFVAALVSVIPRRVTERRKVPARYGRAVLTTLSVAATSAALALVAV
                     DRRSSVLDDNQASLLIGLLAAAVALRILVNQVRGTQSQRAVHDALDQKEAALRETDMA
                     LSRLQRANETLRESEERLRTVFESAVDGIVELDPRDVVVRANGAFCKMVELPQSLVEG
                     QPWSAL"
BASE COUNT          821 a         2338 c         2239 g         1134 t
ORIGIN      
        1 gaacaggatc gccccccgcg caccctggcg agtctgcgtc agcgactgcg ccggcatcac
       61 cccgagcgag aagcccgcgt agacgatgtc ggccgggagc ccttgggccg cctccgaacc
      121 gcggcggatg atctccgaga aaccgacccg cttggcgttg gcgacaccct cgtcgacggt
      181 cccgaacgtc ttgccctcgt agaggtccgg cgcgtggatc ttgtgcccgg cctcccgcag
      241 ctcgtcgacg aactcgagga acccgggcgt ctgccctcgc gcgtggtgaa acaggagcac
      301 cgtcgtcatc gcgagcactg tagccaaacg cggcgccgtc gtcggcgcgg actccccacc
      361 gcccccttca cgcggaccgg tcgcagccct ggagcgagtc acggtaaccg ctccccctcc
      421 gaccgaatct cgcgggcggg gccgacggat ccaaagttcc ttccgtcgga cgctcaggcc
      481 cacgtcggcg cgtgccgaag gtagttggac ggcacgaacg ggtgggcgcc cacgaggcgt
      541 cccccaccgg cggagccaca tctggatctg gccggacgtg aaggcggggc cgccaccccc
      601 tcggcggccc cgccgccttt ttgtcggctc gctccctgcc tcgttccttg ggcctcgtcc
      661 cttgggcctc gttccttggg cctcgacggg cgggccacgc cgcgagcggt gcgcccggcg
      721 ataatcggcc ggatgccgaa cgccgtccga cggatcgggc tcggaaccgt cttcgcgctg
      781 ctcacgggca ccctcgtgct cggtcacgcc ctcaaggcgc cgtgcgcgtc cggcgagtgg
      841 ggcgacaacc gccagtacac gcggctgtgc tactcggaca acgtgcacca gctcggcacc
      901 gagcagctga acgggggccg gctccccttc ctggacgcct gcgcggacac cacggccgaa
      961 tgcgacgagt accccctgct cacgatgtgg acgatgcggg ttgcggcatg gatctcgggc
     1021 ccgagcacca ccggctactt ctacgcgagc gcgatcatgc tgtggatcgc cgcgttcgtc
     1081 atcgcgttct gcctgtacct gctcgtcggc gatcgggttc tgtacttcgc gcttgccccc
     1141 acgctcgcgc tctacgcgac gatgaactgg gacctgctcg cggtcgcgct cgccacggtc
     1201 ggcacgctcg cgtacctgcg acgcagcgac ggctggtcgg gagcgctcct cgggctcggg
     1261 gcggcggcca agatcttccc gatgttcctc gtggtcccgt tcgtcgccgc ccgcttccgt
     1321 ggggaggagc ccgatcgcgg gatccggctc gcgtgggccg cggtggggac gtgggtcgcg
     1381 gtgaacctgc ccttcgtgct gctcgcgccg acgggctggt gggagttctt ccgctacaac
     1441 gcggctcgcc tggccgactg ggacagcctg tggtacctgg cgtgccaacg cacgacgggc
     1501 gagacctgca cgaacacgcg actggtcaac ctggcctccg tcgggctgtt cgtcgcgtgg
     1561 gtcgcggtcg tgtggatcct caaggagcgg cgcgaacccg ggttcccgcg gtggacgctc
     1621 ggtttcccga tcctggtgct cttcctgctg accaacaagg tctactcgcc gcagttcagc
     1681 ctgtggctgc tcccgtggtt cgcgctcgcg ttcccccggt tggggtggtt cgcggcgttc
     1741 gagctcgcgg acgtcgcggt gttcgtcacg cgcttctcgt ggttcggccg ctacctcggc
     1801 atcgacgggg gggtcgccgg gttgccggtc ggcgtgttcg agctcgcggt cgcgatccga
     1861 gcgctgatcc tggtcgcctg cgtgatcggg tgggtccggg gtgcgcgggc gggtccgagg
     1921 ccagccgccc cggacgagga acggccggtc atggcggggg tcgccgcgtg acgtcgcgca
     1981 acgacgcgag cggacccgcg ggtccgcggc cttcgtcggt gacacccggc gcagacgatc
     2041 gggcggtgtc gccttccagg cggacgatcg ggatcggtgc cgtgttcctc ctgctcgccg
     2101 ggaccctggc gatcggtcac gcgatcaaga cgccgtgcgt ccatggcgac tggagcgatg
     2161 gccggccgtt cacgtggctc tgccacacgg acatcatccc gctgctcggc aacgagcagc
     2221 tctacggcga ccgtctcccg tacctcgaac cctgcgagca gacggaaggc acctgcgacg
     2281 agtacccggt cctcacgatc tggatgatgc ggctgaccgc ctcggtggcg cgaccccaca
     2341 acacgcgctt cttctacgcg aacgcgatct tgttgtggct cgccgccttg tggaccgcct
     2401 acctcctcta cacggtggtc gggcgccgag ccctgtactt cgcgctcgca cccaccctcg
     2461 cgatctacgg cacgctgaac ctcgacctgc ttcccgtcgc gctcgccacc ggtggaacgg
     2521 tggcgtacct ccgccggcga gacgtcacct ccggggtcct gctgggactc ggcgccgcgg
     2581 ccaagctcta tccggcgctc ctggtgatcc cgttcgtcgt cggtcggttc cggggccgcg
     2641 aacccgaccg agggatctat ctcgtctggg ccgccgtcgg cgcctggatc gcggtcaatc
     2701 tccccttcgc gctcgccggc acgagcggct ggttggagtt cttccgctac aacgccttca
     2761 gaccggccga ctgggacagc ctgtggttca tcgcgtgcga gcaagcgacg gggatgggct
     2821 gcacgaacac gcgcctggtc aacctggggt ctctcgtgtt gttcctcgga tggctggcga
     2881 tcgtgtggac gagcaaggga cgacgccgac ccggcttcgc gcgatggacc ctcgggttcc
     2941 cgatcctggt gatcttcctc ctgaccaaca aggtctactc gccgcagtac agcgtctggc
     3001 tcttgccgtg gttcgcgctg gcgctgcccc accttcgcct cttcctcgcg ttcgaggccg
     3061 cggacaccgc cgtcttcgtg acgcgtttct cgtggttcgg gctgctcggc gggcatcaca
     3121 acggtttcgc gggcagcccg ctgggcgcct tcgaggccgc gatcctggtg agggctgcga
     3181 tcctggtcgt ctgcgtgatc gcgtggatcc gcgccggcga gccgatctcc cggctcgaac
     3241 gaactccgcc ggaccgaacc gatgcccgcg tcgaggtacc ggtttgacga ccggcacggc
     3301 gtccctcatc gaactctcgg acggtcacga tggatggcgc gagcgcgtcg tccgagccct
     3361 gcgcttttgt ctgttcgcgt tcgtcgcgct ccgcgtggcg atgttcgtga tcggcgtggt
     3421 cggcgtcacc ctgttcccgc cgctcggcgc cgtcggcgtg ccggggtggc ccgcgcctgc
     3481 gcccaacccg gggtggcaga acgcgttcac ggcgtgggag cggttcgacg cgctgtggtt
     3541 ccttcggatc gcggacggcg gctatcggct ccgcgacggg agcgccgcgt tcttcccgct
     3601 ctaccccctc gcgatccgag gcctctccct ggcgctcggg ggccaccccc tcgccgcggc
     3661 gacgatcgtg tcgaacgccg ccttctttgg cggcctcgtc gtcacgtatc tcctcacctc
     3721 gtccgagctg tccgaacgcg ccgcgcgtac caccgtactc ctgatgtgcg tcttcccgac
     3781 cgcgcatttc ttcctcatgc cctacagcga gtcgctgttc ttcctcctgt ccgtcacggc
     3841 gttctggggt gcgcgccggc gaagatgggc gatcgcgggc gcagccgggg cgctggcggc
     3901 cctcacccgg agcatcggga tcgtgatcgc gccggcgctg gtgatcgagg cgctgcacca
     3961 gcgcatcgaa cgccgcgggc cggcatggcc cgggttcctg gcggcggccg ccacgggcgg
     4021 cgggctgctc gcctatctcg gatcctgggg gctccgggcg ggcgagtggc tcgcgccggt
     4081 gacgttccag gcgaactggc agcgctccct gtcgtggccg tgggcgacgc tgtgggacgg
     4141 cacgggcgac gcgttccgtt acctcgacga cctcaacggg ggctactggc tgatcgactg
     4201 gttgatcgtg atcccgatgc tcgccgcgtc ggtatacgcg ctcgtgcgcc tgcggcccgc
     4261 ctacggcgtc tacctgtgga gcgccctgct gatcccgctg acctacgtgt tcccggatcg
     4321 tccgctgatg tcgatgcccc ggttcgtcct gcccctgttc ccggccttct ggggcctggc
     4381 gctcggcctc gagcggcttc ggatcccgcg atcggcggcg ctgggcgtga gcgcggccgg
     4441 cctcgggatc ctctcggtct tgaccgtcaa ctggtactac atcttctgag gcgtcccggc
     4501 gtccatatag gatgggcacc acgccgtcga ggtccggagg gagaagtgac gattcgggta
     4561 gcgatcgccg acgaccactc gctcgtacgg cagggactcc gcaggtacct ggacatggcg
     4621 gaggggatcg aggtcgtcgg cgaggccgcc aacgggcagg aactggtcga cctggtcgcc
     4681 aaggaggaac cggacatcgc cctggtggac atccggatgc ccgagatgga cggtctcgag
     4741 gcagcccgga agatccgcga gacccatccc aagaccgggg tgatcatgct gaccgcctac
     4801 gacgaccgcc agttcgtggt ggaggccgtc cggagcggtg ctcgcggcta cgtcctcaag
     4861 gctcgcgacg ccgagcacct gatccagacc gtccgcctgg tcgccggcgg caacatggtg
     4921 atcgaccccc agctggtggt ggcgctcgca gaggagctct cgcaggccaa ggagcgtgac
     4981 cgccgcgcgg agaccctgac cgcgcgcgag atcgaggtcc tgcagctgct cgcgttcgga
     5041 cacaccaacc gcgacatcgc cgagcggctg tacatctcgc ccgacaccgt gaagacccac
     5101 ctcgagcaca tcttcgagaa gctcggcgcc tcggaccgaa cggcggccgt cgccgaggcg
     5161 ctccggcgcc gcctgatcga gtagccgtcc ccgtgcccgg cggggacggt ccggctcgtg
     5221 aactagtcac cccgggctac ggcggtaccc gccggacggc ctagggcagg tcctccggtt
     5281 gaccccggtc gggtgatgga ggcgcgagcc cgcctgctgc atcatggtcc ttcgaacgat
     5341 gtccacgcag acacagcgca gggctccgtg gttcgcgccc ttcgtcctcc tcgtcgcggg
     5401 cggcggtgtg gcgttcgcct acgcggcgtc gagccgttcg ggctggccgg acgacatccg
     5461 atcccccctc gtcgcgggtt cctgcctcct ctccgcgatc gtcatcctgc tccgatgccg
     5521 gcacaagggc gcgtggcgga gcgccccggc gctcggtctt gggatcgcgg cgatcgcggt
     5581 cgtgagcgcc ggcttgctcg tgaccgacgc caccggatcc gaacctccga ggctcggcct
     5641 gtacgacgtg gggttcctgg cggcgggcgt gctgttcctg atcgccgtgg cgatcgagtt
     5701 ccgcgagcac gtggcccgtg aggaccgccg cgagatcgtg gccgacgtcg cgctgctcac
     5761 cgccgcgatc ggcacgatgt tgttcctgtc cctgcgcccc gactcgatgg cggaccccgc
     5821 gaggtccgcg gagttcgcgc tgatcatcgc ttcgggcgca tcggcctacg gtgccctggc
     5881 gctcgcacga ccgaacgtcg cgcacctcgg catgttcttc gcagtctgcg ggatgtgtgt
     5941 gggtgtgcac gggttcgcga agctgtggct gagcggatcg ttcgaggtcg gaaggcccca
     6001 ggtcgacctg ccgatcgcgt tcgccaccct gttcgtcgcg gcgctcgtca gcgtcatccc
     6061 ccgaagggtg accgaacgac gtaaggttcc ggcgcggtac ggcagggccg tcctcacgac
     6121 cctctcggtc gccgcgacca gcgccgccct cgccctggtc gccgtcgaca ggcgcagctc
     6181 cgtgctcgac gacaaccagg cctcgctcct gatcggcctc ctggcggcgg cggtcgcgct
     6241 ccggatcctc gtgaatcagg tccgagggac gcagtcccag cgcgcggtcc acgacgcgct
     6301 cgaccagaag gaggccgcgc tccgcgagac cgacatggct ctgtcacggc tccagcgcgc
     6361 gaacgagacc ctgcgggagt ccgaggaacg gctccgcacc gtgttcgagt ccgcggtcga
     6421 cggcatcgtc gagctcgacc cccgcgacgt cgtcgtccgc gcgaacggcg ccttctgcaa
     6481 gatggtcgag ctcccgcagt cgctcgtcga aggtcagccg tggagcgcgc tc
//
LOCUS       DATBRK010000145         4628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_457437, whole genome shotgun sequence.
ACCESSION   DATBRK010000145 DATBRK010000000
VERSION     DATBRK010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457437"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VIH70_04430"
     CDS             complement(<1..355)
                     /locus_tag="VIH70_04430"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8115641.1"
                     /translation="MDLDAFRGWLQRYFEAWATNDPVDVSALFAEDAVYSYGPFREET
                     HGRDAIVRAWVEGGVPNDLRTDVEPIAVAGDRGVAHWRVSFLDDAGSMVDVDGILVCD
                     FDAAGRCVLHREWYDR"
     gene            382..1296
                     /gene="ftcD"
                     /locus_tag="VIH70_04435"
     CDS             382..1296
                     /gene="ftcD"
                     /locus_tag="VIH70_04435"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEY8115642.1"
                     /translation="MAETGPLVVCVPNFSEGRQHDVIDAICDALAGGGARLVYRQADP
                     EHNRLDTTVVGSPDAVRASALAGAAKAIELIDMTKHTGGHPRMGAADVIPFVPLSWIS
                     MDEVVELARAFAAELADALDIPVYLYDRAATSPDRASLAEVRRGEFEGLREAVARGER
                     LPDFGPHEIGRAGATAVGARKPLVAFNVYLTGTEDAAKEIARSIRESSGGLPAVRAIG
                     FAVPERGAVTVSMNLVDVDVTSPRVAFETVRDLAAERGMEVRSTEIVGLVPRAALGED
                     DIGYLRLEGFDADEQILERLVSTDGKRR"
     gene            1293..1928
                     /locus_tag="VIH70_04440"
     CDS             1293..1928
                     /locus_tag="VIH70_04440"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEY8115643.1"
                     /translation="MSGRIAEQAVEAYLRGLASDDPTPGGGAVAALSGATGAALISMV
                     CNLTFGKQGYEEAEERMRWMLEEAEAARTEFLELADRDATAFDGVMAAFKMPKETDAE
                     KAVRSTSIQAGYESAARVPLEIAKRAAELMELARETTEIGNAQAASDGACAGQALASA
                     VWCATYNVEINAAALKDPAKAQALRDEVSALRASTAALLDATHAAFASRIG"
     gene            1930..2529
                     /locus_tag="VIH70_04445"
     CDS             1930..2529
                     /locus_tag="VIH70_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEY8115644.1"
                     /translation="MRTFEPGEPAALREIWDRRVWSARPVTVVEDAEEQITLFIPAGA
                     RWMAPFRDGKRLKIQQAEYELVEVPYKAHVLSFAWPGSSAAVLLFYGSDWSPSHWYVN
                     LEDPLRRSPVGFDTLDHKLDVIVELDGSWRWKDEDQFAEIVERGLLDPDDEKRLRAEA
                     EDAVRRIVAREPPFDRDWFGWRPDPAWPTPTLPEGWDRV"
     gene            2576..3805
                     /locus_tag="VIH70_04450"
     CDS             2576..3805
                     /locus_tag="VIH70_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEY8115645.1"
                     /translation="MDLSGAAVRFPGLRREQDGRTVVFADAPGGSQVPDAVIEAMADY
                     LRRSNANAHGTFATSQETDQVIEEAHRAAADLLNADADEVVFGPNATTLLFAISRSIA
                     GSLGPGDEVVVTRLDHDANVRPWVLAAEDAGATVRWVDIREEDVTIDRDSLEAALSER
                     TKVVAFTLASNAVGTVTPAAELVRIVRDRASRAIVACDGVHLAQHRGVDAREIGADLV
                     VCSPYKIFGPHLGILFGRREVLSSLRPYKVRPAEDALPFAFETGTQNHEGFAGWIAAV
                     GYLAELGDGGGRRAAVRAAFERAIVPWEAELSRRFLVGIRAIPSVRLYGIGDPERAAE
                     RTPTFAIRVADQHPAETAKRLAERGIFVWDGNYYALEIMERLGLEDSGGAVRIGFCHY
                     HTPHEVDRVLEELATLG"
     gene            3897..>4628
                     /locus_tag="VIH70_04455"
     CDS             3897..>4628
                     /locus_tag="VIH70_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013323819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8115646.1"
                     /translation="MRILILGGDGYLGWPTSMHFARKGHEVHAVDDYLRRRAHAEAGT
                     DSLTPIGASLPARAEAFREVTGLEIGVTEGDLTDWSVVERLFREFEPEAIVHYGEMPS
                     APYSMIDREHAVFTQTNNVVNTLNVLYAMAEIIPEAHLVKLGTMGEYGTPNIDIEEGY
                     LEVEHNGRSDTLPFPKLPGSMYHLSKVHDSHNIHFACRVWGLRATDLNQGVVYGIETD
                     ETRLDERLLTRFDYDEVFGTALNRFC"
BASE COUNT          703 a         1586 c         1699 g          640 t
ORIGIN      
        1 ggcggtcgta ccactcccgg tgcaggacgc accggccggc ggcgtcgaag tcgcagacca
       61 agatcccgtc cacgtcgacc atcgagcccg cgtcgtccag gaaggacacg cgccagtgcg
      121 ccacgccccg gtcccccgcc accgcgatcg gctcgacgtc ggtccggaga tcgttgggga
      181 cccctccctc cacccacgcc cgcacgatcg cgtcgcgccc gtgcgtctcc tcgcggaagg
      241 gaccgtagga gtacaccgcg tcctcggcga acagcgccga cacatcgacg gggtcgttgg
      301 tcgcccatgc ctcgaagtag cgctggagcc agcctcggaa cgcgtcgaga tccatcccgc
      361 gcatcgtagg cttctgcctc gatggccgag accggacccc tcgtcgtctg cgtccccaac
      421 ttcagcgagg gtcggcaaca cgacgtgatc gacgcgatct gcgacgccct cgccggcggc
      481 ggcgcacggc tcgtctaccg gcaggccgac cccgagcaca accgtctcga cacgaccgtc
      541 gtgggttcgc cggatgcggt gcgcgcgagc gcgctcgccg gcgcggccaa ggctatcgag
      601 ctgatcgaca tgacgaagca caccggcggg cacccccgga tgggtgcggc ggacgtgatc
      661 ccgttcgtcc ccctgtcctg gatctcgatg gacgaggtgg tggagctcgc gcgggcgttc
      721 gcggccgagc tcgccgacgc gctcgacatc ccggtctacc tgtacgaccg agccgcgacc
      781 tcgcccgatc gcgcgagcct ggccgaggtg cgaaggggag agttcgaggg gctgcgcgag
      841 gcggtggcgc ggggcgagcg gttgccggac ttcggcccgc acgagatcgg cagggccggc
      901 gcgaccgccg tcggggcgcg gaagccgctc gtcgcgttca acgtctacct caccgggacc
      961 gaggacgcgg cgaaggagat cgcgagatcg atccgcgaat cgagcggggg cctgccggcg
     1021 gtccgcgcga tcgggttcgc ggtccccgaa cgcggtgccg tcacggtgtc gatgaacctg
     1081 gtcgacgtgg acgtgacgtc gccgcgggtg gcgttcgaga cggtccggga cctcgcggcc
     1141 gaacgcggca tggaggtgcg ctcgaccgag atcgtcgggc tggttccacg cgcggcgctc
     1201 ggcgaggacg acatcgggta tctgcgactg gaggggttcg atgcggacga acagatcctg
     1261 gagcggctcg tgagcaccga cggtaagcgt agatgagcgg gcggatcgcg gagcaggcgg
     1321 tcgaggcgta cctgcgaggc ctcgcgtccg acgaccccac acccggcggc ggcgcggtcg
     1381 ccgccctctc gggcgcgacg ggcgccgcgc tgatctcgat ggtgtgcaac ctcacgttcg
     1441 gcaagcaggg ctacgaggag gccgaggagc ggatgcgctg gatgctggag gaggccgagg
     1501 ccgcccggac cgagttcctc gagctggcgg accgggacgc gacggcgttc gacggtgtga
     1561 tggccgcgtt caagatgccg aaggagaccg acgccgagaa ggcggtccgc tcgacgtcga
     1621 tccaggcggg ctacgagtcg gccgcgaggg tcccgctcga gatcgccaag cgagcggccg
     1681 agctgatgga gctcgcgcgc gagacgaccg agatcggcaa cgcccaggcc gcgtccgacg
     1741 gggcgtgcgc cgggcaagcg ctggcatcgg ccgtctggtg cgcgacctac aacgtcgaga
     1801 tcaacgcggc ggcgctgaag gatccggcga aggcgcaggc gcttcgcgac gaggtctcgg
     1861 cgctgcgggc gagcacggcc gccctgctgg acgcgaccca cgcggcgttc gcctcccgca
     1921 tcggctgacg tgcgcacgtt cgagcccggg gagcccgcgg cgctccgcga gatctgggac
     1981 cgccgggtct ggagcgcccg gccggtgacc gtggtcgagg acgccgagga gcagatcacg
     2041 ctgttcatcc ccgcgggtgc acgctggatg gccccgttcc gggacgggaa acggctgaag
     2101 atccagcagg ccgagtacga gctcgtcgag gtgccgtaca aggcgcacgt gctgtcgttc
     2161 gcgtggccgg ggagctcggc ggccgtgctg ctcttctacg ggtcggactg gtcgccctcg
     2221 cactggtacg tgaacctcga ggatccgctc cggcgctccc ccgtcgggtt cgacacgctg
     2281 gatcacaagc tcgacgtgat cgtggagctc gacgggtcgt ggcgctggaa ggacgaggac
     2341 cagttcgccg agatcgtcga acgtggcctc ctcgaccccg acgatgagaa gcgcctgcgc
     2401 gcggaggccg aggacgccgt ccgccggatc gtggcgcgcg agccgccgtt cgaccgtgac
     2461 tggttcgggt ggcgacccga ccccgcctgg cccacgccga cactgcccga gggatgggac
     2521 cgcgtgtgaa cgatccgggt tgcggggcga cgggcgttcg gcgaggctag gcgccgtgga
     2581 tctctcgggc gccgccgttc gcttccccgg cctccgacgg gagcaggacg gacggacggt
     2641 ggtgttcgcg gacgcgccgg gcgggtccca ggtccccgac gccgtgatcg aggcgatggc
     2701 cgactacctg cggcggagca acgccaacgc ccacggcacg ttcgcgacga gccaggagac
     2761 cgaccaggtg atcgaggagg cgcaccgggc ggccgccgac ctgctgaacg cggatgccga
     2821 cgaggtggtg ttcggcccga acgcgaccac gctgctgttc gcgatctccc gctcgatcgc
     2881 cgggtcgctc ggtccgggcg acgaggtcgt cgtgacgcgg ctcgatcacg acgcgaacgt
     2941 ccggccgtgg gtcctggcgg ccgaggacgc cggcgcgacg gtccggtggg tcgacatccg
     3001 ggaagaggac gtgacgatcg atcgggattc cctcgaggcc gcgctgtccg aacgcaccaa
     3061 ggtggtcgcg ttcacgctcg cgtcgaacgc ggtcgggacc gtcacgcccg ccgccgagct
     3121 cgtcaggatc gtccgcgacc gggcgagccg tgcgatcgtc gcgtgtgacg gggtccacct
     3181 cgcgcagcac cgaggcgtcg acgcgcgcga gatcggcgcc gacctggtgg tctgctcccc
     3241 gtacaagatc ttcggcccgc acctcgggat cctcttcggg cgacgagagg tgctctcctc
     3301 gctccggccc tacaaggtcc ggccggcaga ggacgctctc cccttcgcgt tcgagaccgg
     3361 gacccagaac cacgaggggt tcgcgggctg gatcgcggcg gtcggctacc tggcggagct
     3421 cggcgacggc ggcggccggc gggcggcggt ccgagccgcg ttcgaacgcg cgatcgtccc
     3481 gtgggaggcc gagctgtccc gcaggttcct ggtggggatc cgcgcgatcc cctccgtgcg
     3541 tctgtacggg atcggcgatc ccgagcgcgc ggcagagcgc acgcccacgt tcgccatccg
     3601 tgtcgccgac cagcatccgg ccgagacggc gaagaggctc gccgagcgcg ggatcttcgt
     3661 gtgggacggc aactactacg cgctcgagat catggagcgc ctcgggttgg aggactcggg
     3721 cggggcggtc cggatcgggt tctgccacta ccacacgccg cacgaggtgg accgcgtgct
     3781 cgaggagctc gcgaccctcg ggtagcccga tgcatcccgc gagatgcgcg agcccgcgtt
     3841 gctaggatga cgcggctccg gccacctccg accgaaccgg ggagcgaggg aacggtatgc
     3901 ggatcttgat cctcggcggc gacggctacc tcgggtggcc gacctcgatg cacttcgccc
     3961 gcaagggtca cgaggtgcac gccgtcgacg actacctccg ccgacgagcg cacgcggagg
     4021 cggggacgga ctcgctcacc ccgatcggcg cctcgctgcc ggcccgtgcc gaggccttcc
     4081 gcgaggtcac gggtctggag atcggcgtca ccgagggcga cctgaccgac tggagcgtcg
     4141 tcgagcgtct gttccgcgag ttcgagcccg aggcgatcgt ccactacggc gagatgccga
     4201 gcgcccccta ctcgatgatc gatcgcgagc acgcggtctt cacgcagacg aacaacgtcg
     4261 tgaacacgct gaacgtcctg tatgcgatgg cggagatcat ccccgaggcg cacctggtca
     4321 agctcgggac gatgggcgag tacggcacgc cgaacatcga catcgaggag gggtacctcg
     4381 aggtcgaaca caacgggcgt tcggacacgc tcccgttccc caagctcccg gggtcgatgt
     4441 accacctgtc caaggtccac gactcgcaca acatccattt cgcgtgccgg gtctggggcc
     4501 tccgcgccac cgacctgaac cagggcgtcg tctacgggat cgagaccgac gagacaaggc
     4561 tcgacgagcg cctgctgaca cgcttcgact acgacgaggt gttcggcacg gcgctgaacc
     4621 gcttctgc
//
LOCUS       DATBRK010000146         9508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_166886, whole genome shotgun sequence.
ACCESSION   DATBRK010000146 DATBRK010000000
VERSION     DATBRK010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166886"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1574
                     /gene="ligD"
                     /locus_tag="VIH70_04460"
     CDS             201..1574
                     /gene="ligD"
                     /locus_tag="VIH70_04460"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY8115647.1"
                     /translation="MPARKELLEVAGLEVTVSNPDKVFFPATGHTKLDLVRFYLAVAE
                     AALRGIAGRPMALKRFVNGAEGEFFFQKRAPTSRPDWIETVELSFPSGRTAEEVVVRD
                     AAQLVWIVNLGCIDLNPHPVRADDLDHPDELRVDLDPVPGIGWPQIRDVAMVTKEVLE
                     DFGLVGWPKTSGSRGMHVYARVERRWTFPEVRRAALAIAREVERRAPDLATSRWWKEE
                     RHGVFLDYNQNAKDRTVASAYSVRPTPDARVSTPLRWDEVPGCDPDAFTIDTVPARFA
                     DIGDPSSGIDEAAGSLEPLLELAAQHEAAGFGDAPWPPQYAKQEGEPARVQPSKRRRG
                     TPEKEGVVPPPAPGKKAGPTGRRRTKAPLIEIARAETEAEAMQGFERWKARHPEVAAA
                     LQPADVMVDPMRGRSTAWYRIRVNLRNVPEDERPPQEALEVDYDPWEGMDIEAWRSEE
                     QERRRQR"
     gene            complement(1639..3318)
                     /locus_tag="VIH70_04465"
     CDS             complement(1639..3318)
                     /locus_tag="VIH70_04465"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY8115648.1"
                     /translation="MRRRALLSVVALGLAAALASPAQAATDVRVTVDDTAGSYQLAAG
                     GTDETMTRCSEGRRQQNEPTIAVDPSDTDVVVAGANDYCAAIVNGEVWAGYYRSEDGG
                     ATWANSLVPGYPHDTSVAGEASPVHGTCAASGDPTQAFDADGNLFYGFICFNRSKPIN
                     GGVYVARYSDHGAQYDQTVLVKRGTPSGLFQAGLFQDKINLTTDQTDGDHSGNVYVAW
                     SEYHAFGPTNAVLFARSTDGGDSFSRPFRVTPQEQGTASFADLAVGPDGTVYLTYLTY
                     PSNARPTADVWLVSSTDGGLSFGDPVNVASVELFDSNQFAGSTGTVDCGDGPFACESG
                     FTFSRFFTNSAVAADETGVHVVWASELPSGQNQVFVRNSSDGGATWDDDPVTLDPQEA
                     GHQWFPDVASAGGTISVVFYDSRSDPAYDPDRPPGNTAEGVNSGDVVQAFVARSTDGG
                     VSWTETQVSGAGSNFGWETHGSRRVGFWGDYLYVSSVPGAVNVAWTDSRNLVPGIDPR
                     DGASDNGFGVFQDNCVYDPNDINAPAYITPTIDDPCLDEGGLDQNIYGARL"
     gene            3491..4000
                     /locus_tag="VIH70_04470"
     CDS             3491..4000
                     /locus_tag="VIH70_04470"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8115649.1"
                     /translation="MDVDSLLAFVRFHAWANDRILATATGLSDEEFRRETVLDHGSAF
                     QTLRHLVDVDWSWRELCIGNDVGKTYVWDHGFELDDLPALHAFCLEEDTRLRGYVESL
                     HDEALTESVALGSDPDDVVPRWLIVAHVVNHGTQHRSELARYFTECGHSPGDIDLLDA
                     LHVEWPGGA"
     gene            4058..4957
                     /locus_tag="VIH70_04475"
     CDS             4058..4957
                     /locus_tag="VIH70_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY8115650.1"
                     /translation="MHRLLLDVSSLMYRAFFAWGQELRSPGGRPVGAVHGYLDMVTRL
                     LVTRRPGEVVHVYDHEWRPVARTDMYAGYKANRAEDPEDLPEQFPMLRQVLDLTGMSQ
                     AMTRGWEAEDAIGAICASAAKGDRIEIVSGDRDLIQLVRDPGVKLLFTLRGVSELLDL
                     DEAGVLEKYGVPASRYLEFAILRGDASDALPGVRGVGEKTARALVQTYGSLDEVLEDA
                     RSSEPRPGPLKGKPALRARLVESADYVATMQRLVPVNAIAPVDRWRGERDDEGLDALA
                     EELALKGPIQRMKAALDSLAGKD"
     gene            4965..5861
                     /locus_tag="VIH70_04480"
     CDS             4965..5861
                     /locus_tag="VIH70_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8115651.1"
                     /translation="MVRPLAVGVQLPEVERDVRWPELASMARAAEEVGFDSIWLGDHL
                     LYRDDGRPERGPWDAWTTLAALAASTERVRLGPLVACAAFHPPGMLARMAASIDEVSW
                     GRFVLGIGAGWSETEFRAFGIPFGERASRFEEAFTIVRRLLAGERVTFEGRFERAEDA
                     VLLPEPARRVPMMIGSTGDRVLRAALPHVDAWNTWYTGYGNTPEGFATLNDRLTRLAG
                     DAGRPEGELARSACALVVLDRDAGERPIDEGVVPLEGSTERVAEGLRALAESGADEVI
                     LVLSPITERSIRSMGEVLARLE"
     gene            5904..6644
                     /locus_tag="VIH70_04485"
     CDS             5904..6644
                     /locus_tag="VIH70_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY8115652.1"
                     /translation="MSTWGPLALVGGDELNPGNEPQDEVLARAAGGGPAFVLATAAAR
                     QRPEAAVANAVRWFASLGLAIEELPATRRSDAKSSANVARAREGRFFYLVGGDPGLVP
                     KVLADTPLWDAIVEAWRSGAALAGSSAGAMALGEWTLVRERMPGDERRRYLPALGLVP
                     RVAVLPHFETFGHRWVEIAKRAAPRSDVVLLGVGERTAALHVEGAWRALGDGAVTVIA
                     GGERRRFASGEPIEGVPPPDPNPSAPGS"
     gene            6709..6987
                     /locus_tag="VIH70_04490"
     CDS             6709..6987
                     /locus_tag="VIH70_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor"
                     /protein_id="HEY8115653.1"
                     /translation="MATRKQTQAAKRNIRKAQRAARSKRTIAHMPKSTRRALSQQAAR
                     GRSRAGRAGTALEDRNRTQLYERAKKLNIAGRSRMGKWDLIRAIRRAS"
     gene            complement(7040..7267)
                     /locus_tag="VIH70_04495"
     CDS             complement(7040..7267)
                     /locus_tag="VIH70_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY8115654.1"
                     /translation="MLKAYVLIQTEAGGGSKVRSEIGSITGVRVAQSVTGPYDLIVHA
                     EVADMDALGQMLSDIQLIPGLNRTLTCIVKG"
     gene            complement(7374..8354)
                     /locus_tag="VIH70_04500"
     CDS             complement(7374..8354)
                     /locus_tag="VIH70_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY8115655.1"
                     /translation="MRAVVLDPPGRVEVREVPTPEPAAGQVLVEVAACAVCRTDLHII
                     DGELERPKLPLILGHQAVGTIAAVGDGVADRRPGDLVGVPWLGWADGTCAFCTSGREN
                     LCPNARFTGYDLDGGDADFIVADARFSLPVPPAFEPVAAAPFLCAGAIGYRAKRLAGE
                     VERLGLYGFGNAARLVTQIARFEGRTVFAFTRRGDTETQRLALERGCAWAGDSTEPPP
                     QPLDAAIVFAAAGELVPLALAAVAPGGSVVCAEIHMSDIPSFPYELLWGERVLRSVAN
                     LTRRDGEELLELAARIPLAATTRTFPLERAPEAFEAARSGSDATPVIVPG"
     gene            complement(8357..9166)
                     /locus_tag="VIH70_04505"
     CDS             complement(8357..9166)
                     /locus_tag="VIH70_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8115656.1"
                     /translation="MELRPFGATGLEVPVIGLGTWNVFDVSSQHETETVAVLRSHVDG
                     GTRLVDTSPMYGRAEGVLGRALAALRIRAEVLVATKIWTPSPDEGRLQLEAQLGAFGG
                     RVDVEQIHNLVAWRGHLGWLEAERDAGRIGLLGATHYGEDAFDELVAVMRTGRVQGIQ
                     VPYNPVQRRCEREVLPLAAELGIGVIAMRPFAEGSLLRRPPDDPSVLRALGVRTWAQA
                     LLKWCLSDRRIAVAIPATSRVEHAASNAAAGDPPWFDDDQRALVERWASGS"
     gene            complement(9183..>9508)
                     /locus_tag="VIH70_04510"
     CDS             complement(9183..>9508)
                     /locus_tag="VIH70_04510"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY8115657.1"
                     /translation="WAAAPHYLPQSRNPKVALALLEALRDLTGLEIDTGEIELAARVF
                     EREVTEAIEEDGNLAAYVRRLEEASEVSGDEDDDASALQVASGEDIAAELERFLREHN
                     GSTED"
BASE COUNT         1393 a         3391 c         3374 g         1350 t
ORIGIN      
        1 gagagtcgca ggccgatcat gccgagcggg gcggcggtcc aggcaccgtc cttcaggatc
       61 tccacgctca gcatcccgtc gtcggactcc ccgatccgga gcgtcttgcc ctctctcgta
      121 cggtagaagt ccacgcgcac aggataccgg ttcggcgggc acgggtcgcg gggccccgct
      181 cgccctccta tcgtgtcgat atgcccgctc gtaaggagct cctcgaggtc gccggtctcg
      241 aggtgaccgt ctcgaacccc gacaaggtgt tcttcccggc gaccgggcac acgaagctcg
      301 acctggttcg cttctacctc gccgtcgcgg aggcggcgct ccgcgggatc gccggccgtc
      361 cgatggcgtt gaagcggttc gtcaacggcg ccgagggcga gttcttcttc cagaagcgcg
      421 cgcccacatc gcgccccgat tggatcgaga ccgtggagct cagctttccc tccggccgta
      481 ccgcggagga ggtcgtggtc cgcgacgccg ctcagctcgt gtggatcgtg aacctcgggt
      541 gcatcgacct gaacccgcac ccggtgcgcg cggacgacct cgatcatccc gacgagcttc
      601 gcgtcgatct cgacccggtt ccggggatcg ggtggccgca gatccgagat gtcgcgatgg
      661 tcacgaagga ggtgctcgag gacttcggtc tcgtcggctg gccgaagacc tcggggtcgc
      721 gcggcatgca cgtgtacgcg cgggtcgaac gccgctggac gttccccgag gtgcggcggg
      781 cggcgctcgc gatcgcccgg gaggtcgaac gccgcgcgcc cgacctggcg acgagccgct
      841 ggtggaagga ggagcgtcac ggcgtgttcc tggactacaa ccagaacgcg aaggaccgta
      901 ccgtcgcctc cgcgtactcg gtccgcccga cgcccgacgc tcgcgtctcg acgccgctgc
      961 ggtgggacga ggtccccggg tgcgacccgg acgcgttcac gatcgacacg gtgccggcac
     1021 gcttcgccga tatcggcgac ccctcctcgg gcatcgacga ggctgccggc tccctcgagc
     1081 cgcttctcga gctcgcggcc cagcacgagg cggcggggtt cggcgacgcg ccgtggccac
     1141 cgcagtacgc gaagcaggag ggcgagccgg ctcgcgtcca gccgtcgaag cgccggcgcg
     1201 gcacgcccga gaaggagggg gtcgtcccgc cgcccgcgcc cgggaagaag gccggtccga
     1261 ccggtcggcg acgcaccaag gcgccgctga tcgagatcgc gcgcgcggag accgaggccg
     1321 aggcgatgca ggggttcgag cggtggaagg cgcggcaccc cgaggtcgcc gccgcgctgc
     1381 aaccggccga cgtgatggtc gatccgatgc gaggccgatc gacggcgtgg tacaggatcc
     1441 gcgtgaacct ccgcaacgtg ccggaggacg agcggccacc ccaggaggcg ctcgaggtcg
     1501 actacgaccc gtgggagggc atggacatcg aggcgtggcg cagcgaggaa caggagcgtc
     1561 gcaggcagag gtagccgaac gagcgcaggg cgccggccga cggccggcgc ccctgctcgt
     1621 ggcccccgac gacgcccctt acagacgggc gccgtagatg ttctggtcga gacctccctc
     1681 gtcgaggcat gggtcgtcga tcgtcggcgt gatgtacgcc ggcgcgttga tgtcgttcgg
     1741 gtcgtagacg cagttgtcct ggaagacgcc gaaaccgttg tcggaggccc cgtcccgagg
     1801 atcgatgccg ggaaccaggt tccgcgagtc cgtccacgcg acgttgacgg cgcccggaac
     1861 ggaggagacg tagaggtagt caccccagaa cccgacgcgc cgggagccgt gcgtctccca
     1921 accgaagttg ctcccggcgc cgctcacctg cgtctcggtc cacgagacgc ccccgtcggt
     1981 cgaccgcgcg acgaacgcct gcacgacgtc acccgagttc acgccttccg ccgtattgcc
     2041 cggtgggcgg tccggatcgt aggcggggtc gctcctcgaa tcgtagaaga cgaccgagat
     2101 cgtcccgccc gccgacgcga cgtccgggaa ccactgatgg cccgcctcct gagggtcgag
     2161 ggtgaccggg tcgtcgtccc atgtcgcccc gccgtccgag gagttcctga cgaagacctg
     2221 gttctgcccg ctgggcagct ccgaagccca cacgacgtgg acgcccgtct cgtcggccgc
     2281 gacggccgag ttggtgaaga acctcgagaa cgtgaagccg ctctcgcagg cgaacggtcc
     2341 gtcgccacag tcaaccgtcc cggtcgaacc cgcgaattgg ttcgagtcga acaattcgac
     2401 cgacgcgacg ttgacggggt cgccgaacga aaggccccca tcggtcgacg agacaagcca
     2461 gacgtcggcc gtcggcctag cgttcgaggg ataggtcagg taggtcagat acacggtccc
     2521 gtccggaccg accgcaaggt cggcgaacga cgccgtcccc tgctcctgcg gggtcaccct
     2581 gaagggcctg gagaacgagt cgccgccatc ggtcgaccgc gcgaacaaca ccgcgttggt
     2641 cggcccgaag gcgtgatact ccgaccatgc cacgtacacg ttgcccgagt gatccccgtc
     2701 cgtttggtcg gtcgtgaggt tgatcttgtc ctggaagagt ccggcttgga acaggcccga
     2761 cggggtcccc ctcttcacca gcaccgtctg gtcgtactgc gcgccgtggt ccgagtaccg
     2821 ggccacgtag acgccgccgt tgatcggctt gctccggttg aagcagatga acccgtagaa
     2881 caggtttccg tcggcgtcga aggcctgcgt cggatcaccg gacgctgcac acgttccatg
     2941 aaccggggag gcctcgccag cgaccgacgt gtcgtgggga tacccgggga ccaggctgtt
     3001 cgcccacgtc gctccgccgt cctccgagcg atagtagccc gcccagacct cgccgttcac
     3061 gatcgcggcg cagtagtcgt tcgcaccggc cacgacgacg tcggtgtccg acgggtcgac
     3121 cgcgatggtc ggctcgttct gctgccgacg accttcgctg catcgggtca tcgtctcgtc
     3181 ggtcccgccg gcggccagct gatagctccc ggccgtgtcg tcgacggtca cgcggacatc
     3241 cgtcgccgcc tgtgcgggtg acgccagtgc agccgccagc ccaagcgcga cgaccgagag
     3301 gagcgctcga cgtctcattt cgtacctcct tccccccccg cattcgggga acgcgacttg
     3361 cctggctacc cagccgcgcc ggatcgaaac cttccctggt gagcgggtgc ctttggccgc
     3421 gtgatcgagc tggtccggac ggccaacgtc gagtccgccc gccgctgcga accgcatcta
     3481 ggatgactcc atggatgtcg acagcctcct cgcattcgtc cgcttccacg cttgggcgaa
     3541 cgaccgcatc ctggcgactg cgaccggcct ttccgatgag gagttccgac gcgagacggt
     3601 tctggaccac ggcagcgcct tccagacgct ccgacatctc gtcgacgtcg actggagctg
     3661 gcgcgagctc tgtatcggga acgacgtcgg caagacctat gtgtgggacc acgggttcga
     3721 gctcgacgac ctccccgctc tccacgcctt ctgtctcgaa gaggacaccc gcctgcgggg
     3781 gtacgtggag tccctccacg acgaagcgct gaccgagtcg gtggcgttgg gttcagaccc
     3841 ggacgacgtc gtcccgcgtt ggttgatcgt cgcccatgtc gtgaaccacg ggacgcagca
     3901 tcgcagcgag cttgcgcgct acttcacgga gtgcgggcac tcgccgggcg acatcgacct
     3961 cctggacgca ctccacgtgg agtggccggg cggcgcatga tccgttagcg ctcctcacgg
     4021 acggggaacc aagccggacc cggctacgct ctccgagatg catcgactcc tcctggacgt
     4081 ctcgagcctg atgtaccggg ccttcttcgc ctggggccag gagctccgct cacccggcgg
     4141 gcggccggtc ggcgccgtgc acgggtacct ggacatggtc acccggctcc tcgtcacgcg
     4201 acggcctggc gaggtcgtgc acgtctacga ccacgagtgg cggcccgtcg cgcgaaccga
     4261 catgtacgcg ggctacaagg cgaaccgggc cgaggacccc gaggacctgc cagaacagtt
     4321 cccgatgctg cgacaggtcc tcgacctcac ggggatgtcg caggcgatga cccgcggctg
     4381 ggaggccgaa gacgcgatcg gcgcgatctg cgcgagcgcc gcgaaaggcg atcgcatcga
     4441 gatcgtgagc ggcgaccgcg acctgatcca gctcgtgcgc gacccgggcg tgaagctgct
     4501 gttcacgctg cgcggcgtct ccgagctgct cgacctggac gaggcgggtg tgctcgagaa
     4561 gtacggcgtg cccgcgtccc gatacctcga gttcgcgatc cttcggggcg acgcgagcga
     4621 cgccctgccg ggggtgcgcg gtgtcggcga gaagacggcg cgagcgctcg tccagacgta
     4681 cgggtcgctc gacgaggtcc tggaggacgc ccgctcctcg gagccgaggc ccggcccgct
     4741 caagggcaag ccggcgctcc gagcgcggct cgtcgagagc gccgactacg tggcgacgat
     4801 gcagcggctg gtgccggtga acgcgatcgc gccggtcgac cgatggaggg gcgagaggga
     4861 cgacgagggg ctcgacgcgc tcgccgagga gctcgccctg aagggaccga tccagcggat
     4921 gaaggccgcg ctcgactcgc tcgcggggaa ggactgagcc gccggtggtc cgaccgctgg
     4981 ccgtcggcgt gcagcttccc gaggtcgagc gcgacgtccg ctggccggag ctcgcctcga
     5041 tggcgcgcgc ggccgaggag gttggcttcg actcgatctg gctcggggac cacctgctgt
     5101 accgggacga cggccgcccc gaacgcggcc cttgggacgc gtggacgacg ctcgcggccc
     5161 tcgcggcgtc aaccgaacgc gtccggctcg gcccgctggt ggcgtgcgcc gccttccacc
     5221 ctccggggat gctcgcgcgg atggcggcct cgatcgacga ggtgagttgg ggacggttcg
     5281 tcctcgggat cggcgccggc tggagcgaga cggagttccg ggcgttcggc atcccgttcg
     5341 gcgaacgcgc gtcgcggttc gaggaggcgt tcacgatcgt ccgtcgtctg ctcgccggcg
     5401 aacgcgtgac gttcgagggg cgcttcgagc gcgccgagga cgccgtgctg ctcccggaac
     5461 cggcccggcg ggtcccgatg atgatcgggt ccaccggcga tcgcgtgctg cgcgccgcgc
     5521 ttccgcacgt ggacgcctgg aacacgtggt acacgggcta cggcaacacg ccggaggggt
     5581 tcgccacgct gaacgatcga ctcacccggc tcgccggcga tgcaggccgg cccgagggcg
     5641 agctcgcccg cagcgcgtgt gccctcgtcg tgctcgaccg agatgccggc gagcggccga
     5701 tcgacgaggg cgtcgtgccg ctcgagggat cgacggagcg ggtcgcggag gggctgcgtg
     5761 cgctggcgga gtccggcgcc gacgaggtca tcctcgtcct gtcgccgatc accgaacgct
     5821 cgatccgatc gatgggcgag gtgctcgcgc gcctcgagtg agccatcggg cccgaccacg
     5881 tgcaccgtgg caggatggtc cggatgtcca cgtggggacc gctggcgctc gtcggcggtg
     5941 acgagctcaa ccccggcaac gagcctcagg acgaggtgct ggcccgggcg gcggggggcg
     6001 gaccggcgtt cgtgctcgcg accgcggccg cgcgccagcg ccccgaggcg gcggtcgcga
     6061 acgcggtccg gtggttcgcg agcctcggtc tcgcgatcga ggagctgccc gcgacgcgcc
     6121 ggtccgacgc gaagtcatcg gcgaacgtcg cccgggcccg cgagggccgc ttcttctacc
     6181 tcgtcggcgg cgaccccggg ctggtgccca aggtcctggc ggacacgccg ctgtgggacg
     6241 cgatcgtcga ggcgtggcgc tcgggtgcgg ccctcgccgg ctcgtcggcg ggcgcgatgg
     6301 ctctcggcga gtggaccctg gtccgagagc gcatgcccgg cgatgaaagg cgtcgctacc
     6361 tgccggcgct cgggctcgtc ccgcgcgtcg cggtgctccc gcacttcgag acgttcgggc
     6421 accgctgggt ggagatcgcc aagcgcgccg cgccgcggag cgacgtcgtc ctcctcggcg
     6481 tcggcgagcg tacggccgcg ctccacgtcg agggcgcatg gcgcgcgctc ggcgacggcg
     6541 ccgtcacggt gatcgccggc ggggagcgcc gtcggttcgc ctcgggcgaa ccgatcgagg
     6601 gcgtcccgcc gccggacccg aacccttccg cgccgggttc gtgacgtttc gtcgttcgca
     6661 cacggggaaa ggacacgacg atgggacgac cgcgaggagg cgatccgcat ggccacccgc
     6721 aagcagaccc aggcggcgaa acgcaacatc cggaaggcgc agcgcgccgc ccgttccaag
     6781 cgcacgatcg cgcacatgcc caagagcact cggcgggcgc ttagccagca ggcggcccgc
     6841 ggccgctcgc gcgcagggcg ggccggcacc gcactcgagg accgcaaccg gacgcagctc
     6901 tacgaacggg cgaagaagct gaacatcgcc ggacgctcgc gtatgggcaa gtgggacctg
     6961 atccgagcga tccgacgcgc gagctgacgc tcgccgagct tgcggaggct gccgtcgccg
     7021 atcgacggct tcggggcggc taccccttga cgatgcaggt gagcgtccgg ttcagcccgg
     7081 ggatcagttg gatgtcgctc agcatctgac cgagcgcgtc catgtccgcg acctcggcgt
     7141 ggacgatgag gtcgtagggc cccgtcaccg actgggccac acggacgccg gtgatcgagc
     7201 cgatctcgct ccgcaccttc gacccgccgc ccgcctccgt ctggatcagc acgtacgcct
     7261 tcagcatccg acctccccgc cgcgagtggt catcgtgccc tcagagccgc tcggcgtgca
     7321 accgtgcggg tcacgcgatg cgcggcgcga cccggtacgc ggctgaactc ggctcacccc
     7381 ggaacgatga ccggcgtcgc gtccgagccc gagcgggcag cctcgaacgc ctcgggcgcc
     7441 cgctcgagcg ggaacgtccg ggtggtggcc gcgagcggga tccgcgccgc gagctcgagc
     7501 agctcctcgc cgtcgcgccg cgtcaggttc gccacggagc gaaggacgcg ctccccccac
     7561 agcagctcgt acgggaacga ggggatgtcg gacatgtgga tctcggcgca gacgaccgag
     7621 ccgcccggcg cgacggccgc cagggcgagc gggaccagct cgcccgcggc cgcgaacacg
     7681 atcgccgcgt cgagcggttg cgggggcggc tcggtggagt cgcccgccca cgcgcacccg
     7741 cgttcgaggg ccagccgttg ggtctcggtg tctccgcgcc gcgtgaacgc gaagaccgtc
     7801 cggccctcga aacgcgcgat ctgcgtgacg agccgcgcgg cgttgccgaa cccgtacaga
     7861 cccagccgct cgacctcgcc ggcgagccgt ttcgcccgat agccgatcgc acctgcgcac
     7921 aggaacggcg ccgccgcgac cggctcgaac gccggcggga ccgggaggct gaaccgcgcg
     7981 tcggccacga tgaagtcggc gtcgccgccg tcgaggtcgt agccggtgaa cctcgcgttc
     8041 ggacacaggt tctcgcggcc gctcgtgcag aacgcgcacg taccgtccgc ccacccgagc
     8101 caggggacgc ccacgagatc gccgggccgt cgatccgcga cgccgtcgcc gacggccgcg
     8161 atcgtcccga ccgcctgatg accgaggatc aaggggagct tcgggcgctc gagctcgccg
     8221 tcgatgatgt ggaggtcggt ccggcacacg gcgcacgcgg cgacctccac gaggacctgc
     8281 cccgcggcgg gttcgggcgt cgggacctcg cgtacctcga cccgcccggg agggtcgagc
     8341 accaccgcac gcatagtcac gatccgctcg cccagcgttc gaccagcgcg cgctggtcgt
     8401 cgtcgaacca cggcggatcg cccgcggcgg cgttcgatgc ggcgtgctcg acccgcgacg
     8461 tcgcggggat cgcgacggcg atccgtcgat ccgacaggca ccatttcagg agggcctgcg
     8521 cccacgtccg cacacccagc gcccgcagca cggagggatc gtcgggcgga cggcgcagca
     8581 gggagccctc ggcgaacggc cgcatcgcga tcacgccgat cccgagctcg gccgcgagcg
     8641 ggagcacctc gcgctcgcag cggcgttgca cggggttgta cgggacctgg atgccctgga
     8701 cgcgacccgt ccgcatcacg gcgacgagct cgtcgaacgc atcctccccg tagtgcgtcg
     8761 cgccgagcag cccgatccgg cccgcgtcgc gttcggcttc cagccacccc aggtgcccgc
     8821 gccaggccac gaggttgtgg atctgttcca cgtccacgcg gccgccgaac gccccgagct
     8881 gcgcctcgag ctgaaggcgc ccctcgtccg gcgacggcgt ccagatcttc gtcgcgacca
     8941 gcacctcggc ccggatccgt agggccgcca gcgcccggcc gagcacgccc tcggcgcgcc
     9001 cgtacatcgg ggatgtgtcc acgaggcgcg ttccaccgtc gacgtgcgac cgcagcacgg
     9061 cgaccgtctc cgtctcgtgt tgcgaggaga cgtcgaacac gttccacgtc ccgagaccga
     9121 tcacggggac ctcgagaccc gtcgcgccga acggccggag ctccacccga tccgggggct
     9181 ggtcagtcct cggtcgagcc gttgtgctcg cgcaggaagc gctcgagctc ggcggcgatg
     9241 tcctcgccgc tcgcgacctg gagggctgac gcgtcgtcgt cctcgtcccc gctgacctcc
     9301 gatgcctcct cgagccggcg cacgtaggcc gcgaggttgc cgtcctcctc gatcgcctcg
     9361 gtgacctccc gctcgaacac ccgggcggcg agctcgatct cccccgtgtc gatctcgagc
     9421 ccggtcaggt cgcgaagcgc ctcgagcagc gcgagcgcga ccttcgggtt cctcgactgc
     9481 ggcaggtagt ggggtgcggc cgcccaca
//
LOCUS       DATBRK010000147         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1611666, whole genome shotgun sequence.
ACCESSION   DATBRK010000147 DATBRK010000000
VERSION     DATBRK010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611666"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2753)
                     /locus_tag="VIH70_04515"
     rRNA            complement(<1..2753)
                     /locus_tag="VIH70_04515"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3001..>3109)
                     /locus_tag="VIH70_04520"
     rRNA            complement(3001..>3109)
                     /locus_tag="VIH70_04520"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          505 a         1079 c          835 g          690 t
ORIGIN      
        1 acccttggga cctgctccag ccccaggatg agacgagccg acatcgaggt gccaaaccgc
       61 tccgtcgata tggactcttg ggagcgatca gcctgttatc cccggggtac cttttagccg
      121 ttgagcgacg gccctaccac agagcgccgt cggatcacta ggccctgctt tcgcatctgc
      181 tcgatccgtc gatctcacag tcaagctccc ttgtgccctt gcactcgacg cctgattgcc
      241 aaccaggctg agggaacctt tgggcgcctc cgttacactt taggaggcaa ccgccccagt
      301 taaactgccc accaggcgct gtccccgacc cggatcacgg gccaaggtta gagtcccaac
      361 acgcgaaggg cggtatttca aggttggctc caccccggcc gaaaccaggg cttcgcagcc
      421 tcccgcctat cctacgcaac gcatgccaga acccaacacc aagatgcagt aaaggtcccg
      481 gggtctttcc gtcctgccgc gggtaacgag catctttact cgtagtgcaa tttcgccgag
      541 tccctggttg agacagtgcc caagtcgtta cgccattcgt gcaggtcgga acttgcccga
      601 caaggaattt cgctacctta ggaccgttat agttacggcc gccgtttacc ggcgcttagg
      661 tttggagctt cgccacccga aggtgactga cccctcccct taacgttccg gcaccgggca
      721 ggcgtcagtc cgtatacgtc gtcttgcgac tttgcacgga cctgtgtttt tagtaaacag
      781 tcgcttgggc ctggtttgtg ccaccacgtc gggctccgga cgcgaggtcc ttcaccctat
      841 cgtggtcccc cttctcccga agttacgggg gtaatttgcc gagttcctta accagggttc
      901 tctcgatcgc ctcggtattc tctaccggcc cacctgagtc ggtttggggt acgggcacct
      961 cattcgctcg ctagaggttt ttctcggcag cgtgggatcg gtcgcttcgc ctgaaacggc
     1021 tcggcatcgt gtctcggggt tgacgatccc ccgggtttgc ctgggggacc cccctacgca
     1081 cttgccccac gacaaccatc gcgtgggtcg acctaccctc ctgcgtcccc ccttcgctgc
     1141 cctagtacca ggtctgtcgg tagccccgaa ggggttccct tagtgccctg gattcgggct
     1201 gggcgcggat gaggtggtac tggaatatca accagtcgtc catcgcctac gcctctcggc
     1261 ctcggcttag ggcccgacta accctgggcg gatgaacctt ccccaggaac ccttgggcat
     1321 tcggcggacg ggctcctccc ccgtctttcg ctactcatgc cggcattctc acttctcacg
     1381 cgtccaccgc tgggttaccc cgccgcttca ctcgcgtgag aacgctcccc taccgatccc
     1441 tcggcctgga cgtacgcggc ctcccgcgca cgccgagcca tctgaaggat cccgcagctt
     1501 cggcaccgtg cttgagcccc gctacatcgt cggcgcagga ccacttgacc agtgagctgt
     1561 tacgcactct ttcaagggtg gctgcttcta agccaacctc ctggttgtct cggcaatccc
     1621 acatccttct ccacttagca cggatttcgg ggccttagct ggcgatctgg gttgtttccc
     1681 tctcggctac ggagcttagc ccccgcagcc tcactcccgg gctctggagc atcggcattc
     1741 ggagtttgac tgaggtcggt aagcttgtgg gccccctcac ccaatcagtg ctctacctcc
     1801 gatgctgaac gcccgaggct gcccctaaag gcatttcggg gagaaccagc tatcaccggg
     1861 tttgattggc ctttcacccc tacccacagg tcatccccca ggttttcaac cctggtgggt
     1921 tcggcccttc acgaggtctt accctcgctt cagcctgccc atgggtagat cacccggctt
     1981 cgggtctagg gcgtgcgact gtgcgcccga ttaggactcg ctttcgctcc ggcttcccga
     2041 gatcggttaa cctcgccgca caccgctaac tcgccggctc attcttcaaa aggcacgccg
     2101 tcacccgccc ctggttgccc aggaacgagc tccgacagct tgtaagctca cggtttcagg
     2161 tgctctttca ctcccctccc ggggtgcttt tcacctttcc ctcacggtac tagttcacta
     2221 tcggtcgcca gggagtgctt agccttaggg ggtgggcccc ctagattcgc gctggatttc
     2281 tcgggtcccg cgctactcgg gaacactgca aggagatcgg tcacgtttcg actacagggc
     2341 tgttacctcc tatggcggcc cgttccatgg ccttcgtcta cgtgccgatt ttgtgactcc
     2401 tcgaacacac cggtgtgtgc tcacgcaggt cccacgacac cgatccggca acgccaccgg
     2461 gctatcacac cgggtcggtt taggctgatc cccgttcgct cgccgctact aggggagtcg
     2521 cggttgcttt ctcttcctcg ccctactgag atgtttcact tcgggcggtt ccctcctcag
     2581 gccctatgga ttcagacctg ggtgaccggg catgaccccg gccgggtttc cccattcgga
     2641 caccttcgga tcaaagcctt ctcggcggct ccccgaagct tttcgcagcc agacgcgtcc
     2701 ttcatcggct cctggcgcca aggcatccac cgtaagccct tactaacttg actgtagatg
     2761 ctcgcgctcg ctatcgagtt ctcaaggtgc tcggaggctc gcacccccag agctgaacag
     2821 tgtgcccgcc ggactccccc gctcggaccg gatcgcgttc cactcccctc ctgctcggag
     2881 ggacgtactt gcgaacggct cgagcgagct cgtctgccga gtagccagcg caccgacctg
     2941 gagtcttccg gccgtcgaga cgaacgctcg accagaccgg agtgccaagt ggctccttag
     3001 aaaggaggtg atccagccgc accttccggt acggctacct tgttacgact tcaccccaat
     3061 cgcgagcccc accttcgacg gctccctccc aaagggttgg gccaccggc
//
LOCUS       DATBRK010000148         5934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2478563, whole genome shotgun sequence.
ACCESSION   DATBRK010000148 DATBRK010000000
VERSION     DATBRK010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2478563"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VIH70_04525"
     CDS             <1..876
                     /locus_tag="VIH70_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8115658.1"
                     /translation="YGSGSPANCQYDDTARLIDGADLARVLPLGDNQYEVGAFAAYMS
                     YFDPTWGRAKPNLAPAPGNHEYANDPSSRPRGYFRYFGDAVKGPDGLGYYSYDIGACP
                     ADPCWHLISLNSELCFAAGGCGPAADPSDPGPGNRMYAWLAQDLADHPDSAYPCTLAY
                     WHHPRFSFSTGSGATSTVGPLWRLLHAARADVVLNGHSHNYQRWRPMNPNGDLDRETG
                     IREFVVGTGGASHYALPSGTKPDNLVAAQADAFGVLKLTLKADRYRWRWVSAAGQPAF
                     DDASAHAVRCVRATP"
     gene            complement(866..1648)
                     /locus_tag="VIH70_04530"
     CDS             complement(866..1648)
                     /locus_tag="VIH70_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8115659.1"
                     /translation="MIAVAMIFLLPVVFILLTALMTDQQVLGANLWPNPFRWSNFTEV
                     FHAIPLFTYTRNTFLVASLSTIGVVVSCMPVAYALSRMRWKGREATFLVVLSTMMLPF
                     QVTVIPLYILFVRFGWIGTMKPLIVPLFFGDAFTIFLLRQFFLTIPSELSDAARVDGA
                     SEWQIMTRLIVPLAKPAIAAVALFQFLYSWNDFFAPLLYLGENPKLWTLSIGLSEFRG
                     QFQTQWNLTMAASVLFMLPVIILFFLAQRVFVEGVTLTGVKG"
     gene            complement(1765..2184)
                     /locus_tag="VIH70_04535"
     CDS             complement(1765..2184)
                     /locus_tag="VIH70_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8115660.1"
                     /translation="MIVFLAGLLDVPAHLYEAADIEGAGPLQKFRYVTLPMMSPVIFF
                     PLVIGVIYGFQYFAQAYVASFAVAGSAQSSSAVNVGSPQGSLLFYSLYLYVKGFSNFR
                     MGYASAMAWVLFLITMACTAILIKTSRRWVHYQGGFR"
     gene            2385..2687
                     /locus_tag="VIH70_04540"
     CDS             2385..2687
                     /locus_tag="VIH70_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115661.1"
                     /translation="MADVTEDLGSEVNVLFTVDAPPVETDETLAASEQEGEGEIPLLA
                     VERAVFCARVDPRTSCGPGTPIRLSIDPRRFHFFDRHTGLALSTASVSATAVDSRS"
     gene            2684..3757
                     /locus_tag="VIH70_04545"
     CDS             2684..3757
                     /locus_tag="VIH70_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY8115662.1"
                     /translation="MRSLEGLSDRFLGEIAGQPDAMRRAAAALVDQRDELERIREASE
                     AARTIVFTGMGGSYDACYPAVNDLAARGVAALLVDAAEVLHFRRPILNARTLAIVVSQ
                     SGESAEIVRLVNELAKQRTRPFVIALTNGTNNDLARRADIELDTWAGPESGPSSMTFA
                     ASMTTLSGLARLLAGDSVDTAVDRTRTAAEGAARSAERLLDDPEALAVELEAWHGGRD
                     VIVLLGRGPARAATELGALVLKESGVMAESVEAGLFRHGPLELAGPGMSAIVMATEPE
                     TRRLDLGLAADLVDAGAAVMVVTPDGEAPKGARAVATGYLDRALMSAVGVIPTQLLAW
                     RLAGARGRTPGAYLRASKVTTRE"
     gene            complement(3763..4848)
                     /locus_tag="VIH70_04550"
     CDS             complement(3763..4848)
                     /locus_tag="VIH70_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8115663.1"
                     /translation="MSEVSVLTYRHLVEGIPAVLYVDANDDRSSNLYTSPQIEPLLGF
                     TAEQWRDDPALWLERIHEDDRERVLEEHRRSMRTGDPFLTEYRLLAADGREVWIRDEA
                     VLIRGAEHDPPFWRGVMTDITRTKLVEGRLRTSLDVLRRTMEDRRQLLLRLEDAQEEE
                     RRRIASDIHDDPIQVMSAADIRAEMLARQIEDPELRREAEDLRDVIRSSVERLRHLLF
                     ELRPPALEREGLVPALRACLGDEDPVPEIEDALPFEPPPDLRAILFRIAQEAIANARK
                     HASATRIRVSIAALDGGVRLAVTDDGFGFDVGVIESPEPGHIGLPTMIERAQLAGGAC
                     EVRSAPGGGTTVSAWLPLAEPSSASVG"
     gene            complement(4845..5324)
                     /locus_tag="VIH70_04555"
     CDS             complement(4845..5324)
                     /locus_tag="VIH70_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HEY8115664.1"
                     /translation="MDITAVPFDKPDDLNVIVGQSHFIKTVEDLHEACVNVPGMRFGI
                     AFNEASGPRLVRRSGNDEELVDVAVRNARVIGAGHVFVVTLREGFPVHVLNAVKAVPE
                     VCRVFCATANPLEVVVGETEQGRGVLGVIDGSPPVGVETDADEAERRELLRRFGYKL"
     gene            complement(5374..>5934)
                     /locus_tag="VIH70_04560"
     CDS             complement(5374..>5934)
                     /locus_tag="VIH70_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8115665.1"
                     /translation="AAHAGVEPERGRSAILEAAHKTVALQALNGRWPGVTVNVGMVHG
                     GTRPNVVAERCVLEVDVRSPEEATFEEAGAAVEAIANEHTVPDVSVATISNGWHRPME
                     KGEGGAKLAALAIEVAGELGFGLRDAATGGASDANTTSGAGVPSLDGLGPIGGDDHGP
                     KEWLDLSSVVPRISLLAGLLSRLEGI"
BASE COUNT          914 a         2114 c         2063 g          843 t
ORIGIN      
        1 tacgggtcgg gctcgccggc gaactgtcag tacgacgaca ccgctcgcct gatcgacggc
       61 gccgacctgg ctcgcgtgct gccgctcggc gacaaccagt acgaggtggg cgcgttcgcc
      121 gcctacatga gctacttcga cccgacctgg ggccgcgcca agccgaacct cgcccccgcc
      181 cccggcaacc acgagtacgc gaacgacccg tcctccagac ctaggggcta cttccgttac
      241 ttcggcgacg cggtgaaggg accggacggg ctcgggtact actcgtacga catcggcgcg
      301 tgccccgccg acccgtgctg gcacctgatc tcgttgaact ccgagctctg cttcgcggcg
      361 ggcggctgcg gcccggccgc cgatccctcc gatcccggac cggggaaccg gatgtacgcg
      421 tggctcgcgc aggacctcgc cgaccatccc gactcggcct acccctgtac cctcgcctac
      481 tggcaccatc cgcggttctc gttctccacc gggagcggcg cgacgagcac ggtgggcccg
      541 ctctggcggt tgctgcacgc ggcacgcgcc gacgtcgtgc tgaacggtca ctcgcacaac
      601 tatcagcgct ggcgcccgat gaacccgaac ggcgacctcg accgtgagac ggggatccgc
      661 gagttcgtgg tcgggaccgg cggtgcgagc cactacgccc tgccgagcgg cacgaagccc
      721 gacaacctcg tcgcggcgca ggccgatgcg ttcggcgtcc tgaagctcac gctgaaggcg
      781 gaccggtacc ggtggcggtg ggtgtcggcc gcggggcagc cggcgttcga cgacgcctcg
      841 gcgcacgccg ttcggtgcgt gcgcgctacc ccttgacgcc ggtgagcgtc acgccctcga
      901 cgaacacgcg ctgggcgagg aagaacagga tgatcaccgg gagcatgaac agcacggacg
      961 cggccatcgt caggttccac tgagtctgga actgcccccg gaactcggac aggccgatcg
     1021 agagggtcca cagcttcggg ttctcgccca ggtagagcag cggcgcgaag aagtcgttcc
     1081 acgaatagag gaactggaac agggcgaccg cggcgatcgc cggcttggcg agcggcacga
     1141 tcagccgcgt catgatctgc cactcgctcg cgccgtccac ccgagcggcg tccgagagct
     1201 cggacgggat cgtgaggaag aactggcgga gcaggaagat cgtgaaggcg tctccgaaga
     1261 acaagggcac gatcaacggc ttcatggtcc cgatccatcc gaaccgcacg aacaggatgt
     1321 agagcgggat cacggtcacc tggaacggga gcatcatcgt cgacaggacg acaaggaacg
     1381 tcgcctcccg ccccttccac cgcatgcgag agagcgcgta cgcgacgggc atgcacgaga
     1441 cgaccacccc gatcgtggac agcgaggcga cgaggaacgt gttccgcgtg taggtgaaca
     1501 gtgggatcgc gtggaagacc tcggtgaagt tcgaccagcg gaaggggttc ggccacaggt
     1561 tcgccccgag cacctgctgg tcggtcatca gggccgtcag caggatgaag accacgggaa
     1621 gcaggaagat catcgccacc gcgatcatga ccgcgtggtt cccaaccgac aggaggaagc
     1681 ggcgtcgccg tacctgcggc gggaccgggc gctggaccac cgaggccgcg gtgcgcggtc
     1741 gggccggcgg ggcgagcgtc gtggctatcg gaagcccccc tggtagtgca cccaacggcg
     1801 cgaggtcttg atcaggatcg ccgtgcaggc catcgtgatc aggaacagca cccaggccat
     1861 cgccgaggcg tagcccatgc ggaagttcga gaaccccttc acgtacaggt agagcgagta
     1921 gaacagcagg gacccctggg gggaccccac gttgacggcg gacgagctct gcgccgagcc
     1981 cgcgacggcg aagctcgcga cgtaggcctg cgcgaagtac tggaacccgt agatcacgcc
     2041 gatcacgagc gggaagaaga tgaccggcga catcatcggc agggtcacgt agcggaactt
     2101 ctgcaaaggt cccgcgccct cgatgtcggc tgcctcgtac aggtgcgccg ggacgtcgag
     2161 caggcccgcg aggaagacga tcatggtctg cccgacgccc acaggccgag gaacacgagc
     2221 ccccacttcg acgtcgacgg gtcgaagaac cacagcggtg ggtgatcgac gccgagcccc
     2281 ttcaggatct gattcacggg cccgaccgac gggttcagga tgaacccgcg gacctcgagg
     2341 acgcggccgt ctggtccggg tcgagcctcc cgaccatcac ggtgatggcc gacgtcaccg
     2401 aggatctcgg gtcggaggtc aacgtcctgt tcactgtcga cgcgccgccg gtggagaccg
     2461 acgagacgct cgccgcctcg gagcaggagg gcgagggtga gatcccgctc ctcgccgtgg
     2521 agcgcgccgt cttctgcgca cgcgtggatc ctcggacctc gtgtggtccg ggcacgccga
     2581 tccggctgtc gatcgaccct cgcaggttcc acttcttcga ccggcacacg ggcctcgccc
     2641 tctccacggc ctcggtctcg gccaccgccg tagactcgag gtcatgagat cgctcgaagg
     2701 cctgtccgat cgcttcctcg gcgagatcgc cggtcagccc gacgcgatgc ggcgggcggc
     2761 tgcggcgctc gtggaccagc gcgacgagct cgaacggatc cgggaggcga gcgaggcggc
     2821 ccggacgatc gtgttcaccg gcatgggcgg ctcctacgac gcctgctatc cggccgtgaa
     2881 cgacctggcc gcgcgcggcg tcgcggcgct gttggtcgac gccgctgagg tgctgcactt
     2941 ccgccggccg atcctgaacg cgaggaccct cgccatcgtc gtcagccagt ccggcgagag
     3001 cgccgagatc gtccgcctcg tgaacgagct cgccaagcaa cgtacccggc ccttcgtgat
     3061 cgcgctcacc aacggcacca acaacgacct cgcgcgccgg gccgacatcg agctcgacac
     3121 gtgggcgggc cccgagtcgg gcccctcgtc gatgaccttc gcggcctcga tgaccacgct
     3181 ctcggggctc gcgcggctgc tcgccggcga cagcgtcgac accgcggtcg accggacccg
     3241 gacggcggcg gagggcgcgg cgcggtccgc cgagcgactc ctcgacgacc ccgaggccct
     3301 cgcggtcgag ctcgaggcgt ggcacggcgg tcgggacgtg atcgtactgc tcggccgggg
     3361 cccggcccgg gccgcgaccg agctcggcgc gctcgtgctc aaggagtccg gcgtgatggc
     3421 cgagtcggtc gaggccgggc tgttccgcca cgggccgctg gagctcgccg gcccgggcat
     3481 gagcgcgatc gtgatggcca ccgaacccga gacccggcgc ctcgacctcg gcctcgcggc
     3541 cgatctggtc gacgccggcg ccgccgtcat ggtggtcacg ccggacggcg aggcgcccaa
     3601 gggcgcccgg gcggtcgcga ccggctacct cgaccgggcg ctgatgtcgg ccgtgggcgt
     3661 gatcccgacg cagctcctgg cgtggcggct cgccggcgcg cgaggtcgca cgccgggcgc
     3721 gtacctgcgc gcctcgaagg tcacgacgcg ggagtagccc ggtcagccga cgctcgccga
     3781 gctcggctcg gccagcggca gccacgcgct caccgtggtc ccgccgcccg gcgcgctccg
     3841 gacctcgcat gccccgcccg cgagctgggc gcgttcgatc atcgtcggca gcccgatgtg
     3901 gccgggttcg ggcgattcga tcacgccgac gtcgaacccg aatccgtcgt cggtgaccgc
     3961 cagccgcacg ccgccgtcga gggcggcgat cgacacgcgg atccgggtcg cggaggcgtg
     4021 cttgcgggcg ttcgcgatcg cctcctgtgc gatccggaac aggatcgctc gcaggtccgg
     4081 gggcggctcg aacggcaacg cgtcctcgat ctcgggcacg gggtcctcgt cgcccaggca
     4141 ggcgcgcagc gcggggacga gcccctcgcg ctcgagcgcc ggcggccgga gctcgaacag
     4201 caggtgacgg agcctctcga ccgacgagcg gatcacgtcg cgcagatcct cggcctcccg
     4261 ccggagctcg ggatcctcga tctggcgagc gagcatctcc gcgcggatgt ccgcggcgct
     4321 catcacctgg ataggatcgt cgtggatgtc ggacgcgatc cgcctgcgct cttcctcctg
     4381 cgcgtcttcg agccgcagga gcagctgccg gcggtcctcc atcgttctcc gtaggacgtc
     4441 caagctcgtg cggagccgcc cctcgacgag cttggtcctc gtgatgtcgg tcatgacgcc
     4501 acgccagaag ggcgggtcgt gctccgcgcc gcggataagg acggcctcgt cgcggatcca
     4561 gacctctcgc ccgtcggcgg cgagcagccg gtactccgtc aggaacggat cgccggtgcg
     4621 catcgaccgg cgatgctcct ccagcacacg ttcacggtcg tcctcgtgga tccgttcgag
     4681 ccacagggcg gggtcgtcgc gccactgctc ggcggtgaaa ccgaggagcg gttcgatctg
     4741 cgggctcgtg tagaggttcg acgagcggtc gtcgttcgcg tccacgtaca gcaccgccgg
     4801 gatcccctcg acgaggtgcc ggtacgtcag gacggagacc tcgctcacag cttgtacccg
     4861 aatcggcgca gcagttccct gcgttcggcc tcgtcggcgt cggtctccac accgaccggc
     4921 ggggagccgt cgatcacgcc gagcacgccg cggccctgct cggtctcgcc gaccacgacc
     4981 tcgagcgggt tcgccgtggc acagaagacg cggcagacct ccgggaccgc cttgaccgcg
     5041 ttcaggacgt ggaccgggaa cccctcccgc agcgtgacga cgaacacgtg cccggcgccg
     5101 atcacccgcg cgttccggac ggcgacgtcg acgagctcct cgtcgttgcc gctgcggcgc
     5161 acgagccggg gcccgctcgc ctcgttgaac gcgatgccga acctcatgcc ggggacgttc
     5221 acgcaggcct cgtgcaggtc ctccaccgtc ttgatgaagt gggactgccc gacgatgacg
     5281 ttcaggtcgt cgggcttgtc gaacgggacg gccgtgatgt ccaccggcga accaccctcc
     5341 tcatcgtcgg gcgacctcga gggaccctgg tcgtcagatc ccttcgagcc tggagagtag
     5401 cccggccagc agcgagattc gaggaactac gctcgacagg tcgagccatt ccttcgggcc
     5461 gtggtcgtcg ccgccgatcg gcccgagccc gtcgagcgag ggcacaccgg cgccggaggt
     5521 cgtgttcgcg tccgacgcgc cgccggtcgc cgcgtccctg agcccgaacc cgagctcgcc
     5581 agccacctcg atcgcgagcg ccgcgagctt ggcgccgccc tcccccttct ccatgggccg
     5641 gtgccacccg ttgctgatcg tcgcgacact cacgtcgggg accgtgtgct cgttcgcgat
     5701 cgcctcgacg gccgcgccgg cctcctcgaa cgtggcctcc tcgggggacc ggacgtcgac
     5761 ctcgagcacg catcgttcgg cgacgacgtt cgggcgcgtg ccgccgtgca ccatgcccac
     5821 gttcacggtc acgcccggcc agcggccgtt gagcgcctgg agggccaccg tcttgtgggc
     5881 ggcctcgagg atcgcgctgc ggccgcgttc gggctcgacg cccgcgtggg cggc
//
LOCUS       DATBRK010000149         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1896780, whole genome shotgun sequence.
ACCESSION   DATBRK010000149 DATBRK010000000
VERSION     DATBRK010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896780"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2193)
                     /locus_tag="VIH70_04565"
     CDS             complement(<1..2193)
                     /locus_tag="VIH70_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115666.1"
                     /translation="MALTKDRPDAAVQVPETVRRGWLSSPLVLIGAIALLLVAFGWTF
                     LQDPSISAPTRDPAWYTWRSNLMMHDYPGLIAGDWGPFSMFGGGYRVSVPLMGSILQR
                     VAGIDLYTFSAFLMVGVPVLTGLALGAFSWRTHRDPLLFLLVMLSTAALFMTTPYVGY
                     LDNITVLYFLSLILAFFAPARTSWGARSAMFLIGIAAAFTHPTTCVIFGFSLMAVFGL
                     RLLTSRFHLGPSLKELGPSLMSVGFGMIFGLSTWLIAPWGVTGSLADAALPPPYTKEV
                     FLDRLKGWVGSLQPGILIPLIVLAIAWTIWTARTNRERSDEFGTISAMLLLPLLGIFG
                     FVAATYPYYRFMNATMALFALGGLGAYVAIRWLWRREGAAKVAGVAASLLIVASFGFI
                     WTQGREISHWADPDSQWIDQPTRTALEAARAVVENEPADRPIVFVANYEDTYQSYGWS
                     KTFTNVSRTGLPGDAVKRSMTYFGSVEDFLADRPTVLTNDTYNKMSRAFQRELTDLRE
                     EYTGPPIAILVRQFNEGTVNEELLDRAPDDLVILGDDVAVVTGEGLTTPSDAAIADAR
                     AAEGEVAAFYADHPGIFGNLAHTLWVVFALALLLVVPGAIAARWFGLDDAWLKIALVP
                     GISITLAVLSGIVVVAVTRAPFGAVHGWASLALATAVAAGLRFGASPLLRMLNGFSGF
                     FDSMFSVFSNRDFSVLVGMQYLAQLGQGVVQGAIGKSIAFGGEKGFDVS"
     gene            complement(2286..3968)
                     /locus_tag="VIH70_04570"
     CDS             complement(2286..3968)
                     /locus_tag="VIH70_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8115667.1"
                     /translation="MRRLASLVAVALLAAACTSDTATEASRRTTPSGSSETTAEPVAP
                     PAPAALADAACAIPHRQLVRIWRGTDPERSGQIVFVPREPNFVGTNFPHSGPWDYLQD
                     VPLFWYGPGIIPALGRVERPVTLAGVAPTEARLLDFGFDAPDGHALDEVPKPDTPPPL
                     IVTLVWDAGGDSVLDAFPNDWPVLRSMIDQGIWFDHATVGSSPSITPATHATIGTGAF
                     PMHTGQTDAEFRLGDDLVRAGALGPVLMMEPTLGDLYDRAMGNRPLVAALVSVTWHLN
                     MASHGSLWGGGDRDIAVLRLPTQADNEGAEGTTWNLQGTYQPFYEIPPYVNDLPGLSA
                     YTGALDRQDGALDGNWRDNSISQFEGGWATPARIPYQTRMIEEVISREGFGKDDVPDL
                     LFINSKAIDHISHIFSVNSPEMQDTLSWQDEDLGRFVDFLDREVGTGRYVLIVTADHG
                     AQFDPAVSGAFQVTPTELERDLNAAFPVTGGASVFQAVRSSQIYLNDDVLASSGYTQE
                     EISRFLLEYTHAQGTPDDASPPPAAEASERVFSAAFPISLMRQLDCLPEARP"
     gene            complement(3965..>4322)
                     /locus_tag="VIH70_04575"
     CDS             complement(3965..>4322)
                     /locus_tag="VIH70_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115668.1"
                     /translation="MVLTADHGSIPDPKVTGAFQIAATPIQNGINAAFDTDGDQTRVV
                     QLIQPTQIFVNEAELRQNGHTLDEVSQWILGLTKAETAQPGVTVPAAQADDLVFQAAF
                     PSKIMGHLPCLPEARA"
BASE COUNT          720 a         1498 c         1504 g          600 t
ORIGIN      
        1 cgagacgtcg aaccccttct ctcccccgaa cgcgatcgac ttgccgatcg cgccctgcac
       61 gacgccctgc cccaactgcg ccaggtactg catgccgacc aggacggaga agtcgcggtt
      121 ggagaagacc gagaacatcg agtcgaagaa gccgctgaag ccgttcagca tacgcagcag
      181 cggtgacgct ccgaaccgca acccggccgc gaccgcggtc gcgagcgcca gcgaggccca
      241 gccgtggacg gcgccgaacg gcgctcgggt gaccgccacc acgacgatgc ccgagagcac
      301 cgcgagcgtg atcgagatcc ccggcacgag cgcgatcttc agccacgcgt cgtcgagccc
      361 gaaccaccgc gccgcgatcg ctcccggcac gacgagcagc agggcgagcg cgaacaccac
      421 ccacagcgtg tgcgcgaggt tgccgaagat ccccgggtga tcggcgtaga acgccgcgac
      481 ctcgccctcg gccgcgcgcg cgtccgcgat cgccgcgtcc gagggcgtgg tgagcccctc
      541 gccggtcacc accgcgacgt cgtcgccgag gatcacgagg tcgtccggcg cccggtccag
      601 cagctcctcg ttcacggtcc cctcgttgaa ctggcggacc aggatcgcga tcggcgggcc
      661 cgtgtactcc tcgcgcaggt cggtgagctc gcgctggaac gcgcgtgaca tcttgttgta
      721 cgtgtcgttc gtgagcacgg tcggccggtc ggccaggaag tcctcgacgc tgccgaagta
      781 cgtcatcgag cgcttcacgg cgtcgcccgg caggccggtg cgagagacgt tcgtgaacgt
      841 cttggaccac ccgtaggact ggtacgtgtc ctcgtagttc gcgacgaaca cgatcggacg
      901 gtcggcgggc tcgttctcga cgacggcccg cgccgcctcg agcgccgtcc gcgtcggttg
      961 gtcgatccac tggctgtcgg ggtcggccca gtgcgagatc tcgcggccct gcgtccagat
     1021 gaagccgaac gacgccacga tcaggagcga cgccgcgacg ccggcgacct tggccgcgcc
     1081 ctcgcgcctc cagagccacc ggatcgcgac gtacgcgccg agcccgccaa gcgcgaacag
     1141 cgccatcgtg gcgttcatga accggtagta cgggtaggtg gccgcgacga acccgaagat
     1201 gccgagcaac ggcagtagca gcatcgccga gatcgtgccg aactcgtccg agcgctcccg
     1261 gttcgtgcgc gccgtccaga tcgtccacgc gatcgccagc acgatcagcg ggatcaggat
     1321 ccccggctgc agcgagccga cccatccctt cagccggtcg aggaagactt ccttcgtgta
     1381 cgggggcggc agggcggcgt cggcgagcga gccggtcacg ccccacggcg cgatcagcca
     1441 cgtcgacagg ccgaagatca tcccgaagcc gaccgacatc agcgacggac cgagctcctt
     1501 cagggacggc ccgaggtgga accggctcgt cagcaggcgc agcccgaaca ccgccatcag
     1561 cgagaacccg aagatcacgc acgtcgtcgg gtgcgtgaac gccgcggcga tgccgatcag
     1621 gaacatcgcg ctccgcgcgc cccacgaggt cctcgccggc gcgaagaacg cgaggatcag
     1681 ggagaggaag tacaggaccg tgatgttgtc taggtagccg acgtacggcg tcgtcatgaa
     1741 aagcgcggcc gtcgacagca tgaccagcag gaacagcagc gggtccctgt gcgtccgcca
     1801 ggagaaggcc cccagcgcga ggccggtcag gacggggacg cccaccatca ggaacgcgct
     1861 gaacgtgtac aggtcgatcc cggcgacccg ctgcaggatc gagcccatca ggggcacgct
     1921 cacccggtac ccgccgccga acatcgagaa cgggccccag tcccccgcga tcagaccggg
     1981 gtagtcgtgc atcatcaggt tcgaacgcca cgtgtaccag gcaggatcgc gcgtgggggc
     2041 gctgatcgag gggtcctgga ggaaggtcca gccgaacgcg accagcagga gcgcgatcgc
     2101 gccgatcaga acgagcgggc tcgacagcca gccacgccgc acggtttcgg gaacctgcac
     2161 ggcggcgtcg ggccggtcct tcgtcagtgc catcggtctg cgggatcccc ctcgtcctcc
     2221 gggctcaagc aggtgtcggg cgagcgcggg accggccaag gatacgcgac ggatccacgc
     2281 cgaccctacg gccgggcctc gggcaggcag tcgagctgcc gcatcagcga gatcgggaag
     2341 gccgccgaga acacgcgttc ggaggcctcg gcagcgggag ggggagacgc gtcgtcgggg
     2401 gtcccctgcg cgtgcgtgta ctcgagcagg aaccgcgaga tctcctcctg ggtgtacccg
     2461 ctcgaggcca gcacgtcgtc gttcaggtag atctggctcg agcggacggc ctggaacacc
     2521 gaagcaccgc cggtgacggg gaacgcggcg ttcaggtcgc gctcgagctc ggtgggcgtc
     2581 acctggaacg cgccggagac cgcggggtcg aactgcgcgc cgtggtccgc ggtcacgatc
     2641 agcacgtagc gacccgtgcc gacctcgcgg tccaagaagt cgacgaaccg cccgaggtcc
     2701 tcgtcctgcc acgacagcgt gtcctgcatc tcggggctgt tcacgctgaa gatgtggctg
     2761 atgtggtcga tcgccttcga gttgatgaag agcaggtccg gcacgtcgtc cttcccgaac
     2821 ccctcgcggg agatcacctc ctcgatcatc cgagtctggt aagggatccg ggccggcgtc
     2881 gcccaacctc cctcgaactg cgagatcgag ttgtcccgcc agttcccgtc cagcgcaccg
     2941 tcttggcggt cgagcgcgcc cgtgtacgcg gacaggcccg gaagatcgtt cacgtacggg
     3001 gggatctcgt agaagggttg atacgttccc tgcaggttcc acgtcgtgcc ctcggcaccc
     3061 tcgttgtccg cctgggtcgg caggcggagc accgcgatgt cacggtcccc accgccccac
     3121 agcgacccgt ggctcgccat gttgaggtgc cacgtcacac tcacgagcgc ggccacgagg
     3181 ggccggttac ccatcgcgcg gtcgtacagg tcgccgagcg tcggctccat catcaggacc
     3241 ggtccgagcg cgccggcgcg caccagatcg tcgcccaggc ggaactcggc atcggtctga
     3301 ccggtgtgca tcgggaacgc cccggtccct atcgtcgcgt gggtggccgg cgtgatcgag
     3361 ggagacgaac caaccgtcgc gtgatcgaac cagatcccct ggtcgatcat cgatcgcagc
     3421 accggccagt cgttggggaa cgcgtcgagg acgctgtcgc cgccggcatc ccagaccagc
     3481 gtgacgatca ggggcggcgg ggtgtccggc ttggggacct cgtcgagcgc gtggccgtcc
     3541 ggggcatcga acccgaaatc cagcagcctg gcctcggtgg gcgcgacgcc ggcgagcgtc
     3601 accggacgct ccaccctgcc gagcgccggg atgatgccgg gcccgtacca gaacagcggc
     3661 acgtcctgca ggtagtccca cggccccgag tgcgggaagt tcgtaccgac gaagttcggc
     3721 tcccgcggca cgaacacgat ctgcccagaa cgctcggggt cggtcccccg ccagatccga
     3781 acgagctgcc gatgggggat cgcgcaggcg gcgtcggcca gcgcagcggg cgcgggcggc
     3841 gccaccggct cggccgtcgt ctcgctcgag ccggacggcg tcgtcctcct cgacgcctcg
     3901 gtcgcggtgt ccgaggtgca cgcggccgcc agcagcgcca cggcgaccag agacgcgagc
     3961 cgcctcatgc gcgcgcttcg ggcaggcagg ggaggtggcc catgatcttg gacgggaagg
     4021 ccgcctggaa cacgaggtcg tcggcctggg ccgccggaac cgtgacgccg ggctgcgcgg
     4081 tctcggcctt cgtcagcccg aggatccact gcgagacctc gtcgagcgtg tgaccgttct
     4141 gccggagctc ggcctcgttc acgaagatct gggtgggctg tatcagctgg acgacgcggg
     4201 tctggtcgcc gtcggtgtcg aacgccgcgt tgatgccgtt ctggatcggc gtggcggcga
     4261 tctggaaggc gccggtcacc ttcgggtcgg ggatcgaccc gtggtcggcc gtgagcacca
     4321 tg
//
LOCUS       DATBRK010000150         8112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1612090, whole genome shotgun sequence.
ACCESSION   DATBRK010000150 DATBRK010000000
VERSION     DATBRK010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612090"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VIH70_04580"
     CDS             <1..621
                     /locus_tag="VIH70_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8115669.1"
                     /translation="ERAKQLERNLHDGAQQQIVALTVKLRLLGQLMDRDVEKAKSMAS
                     DLQTDATDALEQLRDLARGIYPPLLADQGLVAALEAQARKAPVPTEVRSDGIGRYPQD
                     IEAAVYFCVLEALNNVAKYAEATAAEVNLSQIDGHVRFAVIDDGTGFDAAETSYGTGV
                     QGMADRLDAIGGALHVVSRKGEGTTIEGRIPTSAEPEVADAPPRSG"
     gene            676..1254
                     /locus_tag="VIH70_04585"
     CDS             676..1254
                     /locus_tag="VIH70_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8115670.1"
                     /translation="MGSVIYDKSASLDGFIAGANVRLEAGLGDDGERLHDWAFQDDAG
                     KEMLEGEIARLGAVICGRRTYDMSIQYWGADGPTGAARVPLFVLSHGVPDDVPDGGVY
                     TFVDDLQTALARARETAGDRNIGVMGADVGRQLIDAGAIDEISVHLVPVLFGSGTPMF
                     GEGVDEHVTLEFVGVSESPHATHLRYRVVRDG"
     gene            1405..2160
                     /locus_tag="VIH70_04590"
     CDS             1405..2160
                     /locus_tag="VIH70_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8115671.1"
                     /translation="MATYVLVPGAWLGGWAWDDVADRLETRGHEAHPVTLTGLGDRAP
                     LATPDIDLERHVGDIAESIEEADLSEIVLVGHSYGGIPVTGAADRIPERIATVVYVDS
                     GPALDGTAYIDTLPPPLKEATERHVAEEGDGWRLPMPSWEELETLNGAGLQGLDEETR
                     RRMRERATPQPFGTYTQAIALKNPARTSLPHVLISCSFPLEQVREFIVSGHPWFAELG
                     GPQWSFVELPTSHWPMFSAPDELARALDEIAPA"
     gene            complement(2157..2411)
                     /locus_tag="VIH70_04595"
     CDS             complement(2157..2411)
                     /locus_tag="VIH70_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY8115672.1"
                     /translation="MRSKEVSEMIETILAVTGWHGGPWFLLFPLFWIALLVGAWFLFR
                     RRDERGRTYSAEEILGERYARGEITVEEYRQRREVLRGGR"
     gene            2576..3745
                     /locus_tag="VIH70_04600"
     CDS             2576..3745
                     /locus_tag="VIH70_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005320568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8115673.1"
                     /translation="MAMNPVHDPEMAARRARRVDAVIALGLAVFLLVGTYFASQGQPA
                     RRGFDAGTVVLLLAAAGALAFRRRWPVGVLCLVFGVSLTYFVLNYPAGPIWSTLIVAS
                     FTAVVEGHLRAAAIVAATGFVVFPWLDFLLRDKPAPPIANLVGLAAWLLVILGAGWFV
                     RVRRERFAEAARMRHEEGLRRTSEERLRIARELHDALGHQLSLINVQSGVALHLNEEL
                     PEQTRASLTAIKEASKEALGELRSVLEILRQGGEPAPRTPALTLDRLDDLASQAKTAG
                     LEVRTQTEGEARPLPFGVESAAFRIIQEALTNVTRHARGATATVHITYGAEDLVVQID
                     DDGRGPPAPNATVGGGRGILGMRERAAALGGELEAGPRPEGGFHVKATLPLDGAA"
     gene            3742..4692
                     /locus_tag="VIH70_04605"
     CDS             3742..4692
                     /locus_tag="VIH70_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015620374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8115674.1"
                     /translation="MIRVLLADDQALVRAGFRALLDAQPDIEVVGEVEDGEGALRRAV
                     ELTPDVLLMDIRMPGMDGLQATRRILQEERLDRTRVVILTTFDEDDYVYEALRLGASG
                     FLVKDTEPDALLQGVRTVARGDPLLSPGITRRLIGALTAREADAFPSASEPDRILATV
                     MFSDIVSSTERAAEIGDRSWRDVLDRHDELVRKLLTEHGGREIKTTGDGFLALFDAPT
                     RAIRCALAIRDELRALEIDVRIGMHSGEVELRGNDVGGIAVNLGSRVAAAASPGEVLV
                     SSTVRDLVGGSGIGFADRGDHELKGVPDRWRLFAVTVPRG"
     gene            4719..5384
                     /locus_tag="VIH70_04610"
     CDS             4719..5384
                     /locus_tag="VIH70_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010352972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8115675.1"
                     /translation="MIRVLLADDQALVRAGFRSLLDAVDDIEVVGEASDGEEAVRLAT
                     ELEPDVALMDIRMPGLDGLAATRAIVEDERLADVRIVILTTFGLDEYVFEAIRSGASG
                     FLVKDTEPEELIQAVRVVAAGDALLSPGVTKRLLAEFATHAKQPPPTGGLDELTERER
                     EIVALVGEGLSNREIAERLFLSPATAKTHVSRAMGKLRVRDRAQLVVIAYESGLVRPG
                     WLD"
     gene            complement(5381..6190)
                     /locus_tag="VIH70_04615"
     CDS             complement(5381..6190)
                     /locus_tag="VIH70_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY8115676.1"
                     /translation="MSDVRRQQQKGSAFQAMHAGDPFVIPNPWDVGSARVLEALGFTA
                     LATTSSGFAFTLGLVDGRVTLDQMAQHVAAIDEATDLPVSADLENGYGPDPADAAEAI
                     RRVANAGAVGGSIEDFDRESRRLYELGHAAERIRAAVEAAHGVAFPFTLTARAENHIR
                     GNADLADTIRRLRSFEEAGADVLYAPGLGTVEEIRALCDAVSKPVNVLAVPGLSMGEI
                     VDAGAQRISGGGSLTWVAVKAFADAAGAIRDRGDLSVLDARVPLDDWLGET"
     gene            complement(6216..6905)
                     /locus_tag="VIH70_04620"
     CDS             complement(6216..6905)
                     /locus_tag="VIH70_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8115677.1"
                     /translation="MATFVLIHGAGSDSWYWHLVVPELIARGHDVVTPDLPSDDDAAG
                     LREYADAVVDAIGDRTDLVVVAQSLAGFTAPLVCVRVPVSLMVLVAAMVPSPGESAGE
                     WWANTGHEQARREQALADGRPPDAGFDPMVDFFHDVPADVVEEAFARGERAQSEAVFG
                     DPWPLESWPDVPTRFLLCLHDRFFPAELQRRVVADRLGITPDEMDGGHLPALARPKEL
                     AERLVAYADER"
     gene            7137..7655
                     /locus_tag="VIH70_04625"
     CDS             7137..7655
                     /locus_tag="VIH70_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115678.1"
                     /translation="MNEDSEYEQAHQEARVATEGALASADRAMADADRLKAEALTGEM
                     LHEGLRQQGLIYGGLIGIAVVMVQPFLIATSLDTSATICIVAFSIAIPFLAALVMVNR
                     QEVFRGRRTPSVTVTVTQAIAQLAAFVGIVAGFWHISWVAGVVMLVAALVAMGVHSAG
                     FVRLEVTRKPTP"
     gene            7804..>8112
                     /locus_tag="VIH70_04630"
     CDS             7804..>8112
                     /locus_tag="VIH70_04630"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEY8115679.1"
                     /translation="MADDTQAQLPTPDPALRRLDRFVGTWSMEGHLVGSDEITVRGET
                     TFRWLPGGFFLEQHAALNFLGLEIESLELIGYDPETGTFPSTVFSNLSPTPLPYRWEV
                     D"
BASE COUNT         1249 a         2755 c         2950 g         1158 t
ORIGIN      
        1 gaacgcgcca agcagctcga gcggaacctg cacgacggcg cgcagcagca gatcgtcgcg
       61 ctcacggtga agctgcgact gctcgggcag ctgatggatc gtgacgtgga gaaggccaag
      121 tcgatggcct cggacctcca gacggacgcg accgatgcgc tggagcagct ccgagacctc
      181 gcgcgcggca tctacccgcc gctgctcgcc gaccagggcc tcgtcgccgc actcgaggct
      241 caggcgcgca aggctcccgt cccgaccgag gttcgatccg acgggatcgg ccgctacccg
      301 caggacatcg aggccgccgt ctacttctgt gttctcgagg ccctgaacaa cgtggccaag
      361 tacgccgagg cgacggcggc ggaggtgaac ctgtcgcaga tcgatggcca tgtccgtttc
      421 gccgtcatcg acgacgggac cggtttcgac gcggccgaga cgagctacgg aacgggggta
      481 caggggatgg ccgaccgctt ggacgcgatc ggcggcgccc tccatgtggt ctcccgcaaa
      541 ggcgaaggca ccacgatcga aggccggatc ccgacgagcg ccgagcccga ggtcgcggat
      601 gcgcctcctc gctctgggta gaagcctcac cgcaggagga ccggcgagga gaaccgagac
      661 cggaggaggc acacgatggg cagcgtgatc tacgacaaga gcgcgtccct cgacggtttc
      721 atcgccgggg cgaacgtgcg gctcgaggcg ggactcggcg acgacgggga gcggctccac
      781 gactgggcgt tccaggacga cgccggcaag gagatgctgg agggcgagat cgcccggctc
      841 ggcgcggtga tctgcgggcg ccgcacgtac gacatgtcga tccagtactg gggcgccgac
      901 ggtccgaccg gggcggctcg cgtcccgctg ttcgtgctgt cgcacggcgt gccggacgac
      961 gtccccgatg gcggcgtgta cacgttcgtc gacgacctgc agacggcgct cgcgcgggcg
     1021 cgcgagactg cgggcgacag gaacatcggc gtcatgggcg ccgacgtggg acgccagctc
     1081 atcgacgccg gcgcgatcga cgagatctcg gtgcatctcg tgcccgtgct gttcgggagc
     1141 ggcacgccga tgttcggcga gggcgtcgac gagcacgtga cgctcgagtt cgtcggggta
     1201 agcgagagcc cgcacgccac gcacctgcgc taccgcgtcg tccgcgacgg ttgaccgggc
     1261 acctgcgcgt cagggggccg gaggcgcgat gagaccaagg tgagtcggag atgaacggcg
     1321 gatgagcacg tgtctcggtc cgtcgccgac ggggtagggg ttgagagggg gcaaggtctc
     1381 gtggttccgg aaggagtgat cgacatggcg acctacgttc tcgtgcctgg ggcatggctc
     1441 ggcggctggg cctgggacga cgtggcggat cggctggaga cacgcgggca cgaggcccac
     1501 cccgtgaccc tgacggggct gggcgatcga gctccgctcg cgacgcccga catcgatctc
     1561 gaacgacacg tcggcgacat cgcggagtcg atcgaggagg ccgacctgag cgagatcgtc
     1621 ctcgtcggtc acagctacgg cgggatcccg gtgacgggcg ccgcggatcg gatccccgaa
     1681 cggatcgcga ccgtcgtcta cgtcgacagc gggccggcgc tcgacgggac cgcgtacatc
     1741 gacacgctgc ctcctcccct caaggaggcg accgagcggc acgtcgccga ggagggcgac
     1801 ggctggcgcc tgcccatgcc gtcgtgggaa gagctggaga cgctcaacgg cgccggcctg
     1861 caggggctcg atgaggagac acggcggcgc atgcgcgaac gagcgacacc ccaaccgttc
     1921 ggcacgtaca cgcaggcgat cgcgctgaag aacccggcca ggacgagcct cccgcacgtc
     1981 ctgatctcgt gcagcttccc gctcgagcag gtcagggagt tcatcgtgag cggacacccg
     2041 tggttcgcgg agctcggcgg gccgcagtgg tcgttcgtgg agctgccgac cagtcactgg
     2101 ccgatgttct cggcgccgga cgagctcgca cgagcactcg acgagatcgc ccccgcctag
     2161 cggccgcccc gaaggacctc gcgccgctgc cgatattcct cgaccgtgat ctccccgcgt
     2221 gcgtagcgct cgcccaggat ctcttcggcc gagtacgtgc gccctcgctc gtcacggcga
     2281 cggaacagga accacgcacc gacaagcagg gcgatccaga agagcgggaa cagcaggaac
     2341 cacggtccgc cgtgccagcc tgtcacggcc aggatcgtct cgatcatctc ggacacctcc
     2401 ttcgatctca ttcatcctcg gcccgagggg tccctgcgtc gtcggccccg gggaggcttc
     2461 gggggcactc ccggagggaa ggatggccac cagccggata caccaggagt tgtacgcgcg
     2521 gccgctcccg gccgccgatg acgccgctcg gcgccgaacc taggctgcga tcgagatggc
     2581 gatgaacccg gtgcacgacc cagagatggc ggcgcggagg gcccggcgcg tggatgccgt
     2641 gatcgccctc gggctcgctg tgtttctcct cgttgggacc tacttcgcct cgcagggtca
     2701 gccggcgcgg cgggggttcg atgcgggcac ggtcgtcctc ctgctcgccg cggcgggcgc
     2761 gctcgccttc cggcgtcgat ggcccgtcgg cgtgctgtgc ctcgtcttcg gggtgtccct
     2821 gacgtacttc gtcctgaact acccggcggg cccgatctgg agcacgctca tcgtcgcctc
     2881 gttcacggcc gtggtcgagg ggcacctgcg ggccgcggcg atcgtcgcgg ccacggggtt
     2941 cgtcgtcttc ccgtggctcg acttcctgct ccgggacaag ccagctcccc cgatcgcgaa
     3001 cctcgtcggc ctggccgcct ggctcctcgt gatcctgggc gccggctggt tcgtgcgcgt
     3061 ccggcgggag cgcttcgccg aggccgcgcg gatgcgccac gaggaggggc tccgccgcac
     3121 gagcgaggaa cggttgcgga tcgcccgtga gctccacgat gcgctcggtc accagctgtc
     3181 cctgatcaac gtccaatcgg gtgtcgcgct ccatctgaac gaggagctgc cggagcagac
     3241 gcgggcctcg ctcaccgcga tcaaggaggc gagcaaggag gcgctgggcg agctccgctc
     3301 ggttctcgag atccttcggc agggcggcga gccggccccg cgaacccccg cgctgacgct
     3361 cgaccggctc gacgacctcg cgtcgcaggc gaagacggcc ggactcgagg ttcgaacaca
     3421 gaccgagggc gaggcgcgcc cgctgccgtt cggcgtcgag tcggccgcgt tccgcatcat
     3481 ccaggaggcg ctgacgaacg tgacccgcca cgcgcgcggg gccaccgcga cggtgcacat
     3541 cacctacggt gccgaagatc tcgtcgtgca gatcgacgac gacggccgcg gcccgcccgc
     3601 gccgaacgcg acggtgggag gcggcagggg gatcctcggc atgcgcgagc gtgccgcggc
     3661 gctcggcgga gagctcgagg ccgggccgcg gccggagggc ggcttccacg tcaaggccac
     3721 gcttcccctg gacggcgccg cgtgatccgg gtcctgctcg ccgacgacca ggcgttggtc
     3781 cgggcgggct ttcgggcgct gctcgacgcc cagccggaca tcgaggtcgt gggggaggtc
     3841 gaggacgggg agggcgccct ccggcgggcg gtcgagctga ccccggacgt cctgctgatg
     3901 gacatccgga tgccgggcat ggacgggcta caggccacgc ggcggatctt gcaggaggag
     3961 cgcctggaca ggacgcgggt cgtgatcctg acgacgttcg acgaggacga ctacgtctac
     4021 gaggcgcttc gcctcggggc gagcgggttc ctggtcaagg acaccgagcc ggacgcgctc
     4081 ctgcagggcg tccggaccgt ggcccgagga gacccgctgc tgtcgcccgg catcacccgg
     4141 cggctgatcg gggctctgac cgcgcgagag gcggacgcgt tcccgtctgc gtccgaaccg
     4201 gaccggatcc tggcgaccgt gatgttcagc gacatcgtct cctcgaccga acgagcggcg
     4261 gagatcggcg accggagctg gcgcgacgtg ctggatcgac acgacgaact ggtgcgcaag
     4321 ctcctgaccg aacacggcgg tcgcgagatc aagacgaccg gcgacggctt cctcgcgctg
     4381 ttcgacgcgc cgacccgcgc gatccgctgc gcgctggcga tccgcgacga gctgcgggcg
     4441 ctcgagatcg acgtgcggat cgggatgcat tcgggcgagg tggagctgcg agggaacgac
     4501 gtgggaggga tcgcggtgaa cctcggctcc cgagtggccg ccgccgcgtc acccggcgag
     4561 gtcctcgtgt cgagcacggt ccgcgacctg gtgggagggt cggggatcgg gttcgcggat
     4621 cgcggcgacc acgagctcaa gggcgtcccg gatcggtggc ggctgttcgc cgtcaccgtt
     4681 ccacggggat gaacaccggt ggcggtggcg agcgctcgat gatccgcgtg ctgctggccg
     4741 acgatcaggc gctggtgcgg gcggggttcc ggtcgctcct ggacgcggtg gacgacatcg
     4801 aggtggtcgg cgaggcgagc gacggcgagg aggccgtgcg acttgccacg gagctcgagc
     4861 ccgacgtcgc gctgatggac atccggatgc cggggctcga cggcctggcc gccacgcgcg
     4921 cgatcgtcga ggatgaacgc ctggccgacg tgaggatcgt gatcctgacc acgttcggcc
     4981 tggacgagta cgtgttcgag gcgatccgct cgggagcgag cggcttcctc gtcaaggaca
     5041 ccgaaccaga ggagctgatc caggccgttc gagtggtcgc ggcgggcgac gccctcctgt
     5101 cgccgggcgt gaccaagagg ctgctcgccg agttcgcgac gcacgcgaag cagccacccc
     5161 cgaccggcgg gctcgacgag ctcaccgaac gcgaacgcga gatcgtggcg ctggtgggag
     5221 agggcctgtc gaaccgggag atcgccgagc ggctgttcct gagcccggcg acggcgaaga
     5281 cccacgtgag ccgcgcgatg ggcaagctcc gcgttcggga ccgcgcgcag ctcgtcgtga
     5341 tcgcctacga gtccggcctc gtccgccccg gctggctcga ctaggtctcg cccaaccagt
     5401 cgtccagcgg cacccgcgcg tcgaggacgg agagatcacc ccggtctcgg atcgcgccgg
     5461 ccgcgtcggc gaacgccttg acggcgaccc atgtgaggct cccgccgccg ctgatccgct
     5521 gcgctcccgc gtccacgatc tctcccatag acagtccggg aacggcgaga acgttgacgg
     5581 gcttcgagac ggcatcgcac agcgcgcgga tctcctcgac cgttcccagc cccggcgcgt
     5641 acaggacgtc ggcgccggct tcctcgaacg atcggagccg ccggatcgtg tcggcaaggt
     5701 cggcgttgcc ccggatgtgg ttctccgcac gggccgtgag cgtgaacggg aaggccacgc
     5761 cgtgcgcggc ctcgacggcg gcccggatcc gctccgcggc gtggccgagc tcgtagagcc
     5821 gtcggctctc ccggtcgaag tcctcgatcg aaccgccgac cgcccccgca ttcgcgaccc
     5881 gtcggatcgc ttcggccgcg tccgcgggat ccggtccata gccgttctcg agatcggcgg
     5941 agacggggag atccgtcgcc tcgtcgatgg ccgcgacgtg ctgcgccatc tgatcgagcg
     6001 tgacccgccc gtcgacgagc ccgagcgtga aggcgaagcc cgagctcgtc gtcgcgagcg
     6061 cggtgaaccc gagtgcttcc aggacgcggg cggatcccac gtcccacggg ttcgggatca
     6121 cgaacgggtc gccggcgtgc atcgcctgga aggcggatcc cttctgttgc tgcctcctca
     6181 catcggacat ggttcaccac cttcccgacc gagactcacc gttcgtccgc gtaggctacg
     6241 agccgttcgg ccagctcctt cggacgggcg agcgccggca ggtggccgcc gtccatctcg
     6301 tcgggcgtga tgccgagccg atcggcgacc acccggcgct ggagctcggc cgggaagaag
     6361 cggtcgtgga gacacaggag gaaccgcgtc ggcacgtcgg gccaggactc gagcggccag
     6421 ggatccccga acacggcctc cgactgagcc cgctcgcccc tcgcgaacgc ctcctcgacc
     6481 acgtccgccg gcacgtcgtg gaagaagtcg accatgggat cgaaaccggc atcgggcggg
     6541 cggccgtcgg ccagcgcctg ctcgcggcgg gcctgctcgt gaccggtgtt ggcccaccac
     6601 tcgcccgccg actcgccggg cgacggcacc atcgcggcca cgagcaccat caggctcacc
     6661 ggcacgcgaa cgcagacgag cggcgccgtg aaaccggcga gggactgggc caccacgacg
     6721 agatcggttc gatccccgat cgcgtcgacg accgcgtcgg cgtactcgcg caggccggcg
     6781 gcatcgtcgt cgctgggaag atcgggcgtc acgacgtcgt gcccccgcgc gatcagctcc
     6841 ggcacgacca gatgccagta ccaggagtcc gaaccggccc cgtggatcag gacgaaggtt
     6901 gccaaaagaa gacctcacac cgggctcgcc gtgcttcgcc tcccgtggtc gaggcgttgt
     6961 ctcgtactcg agggacggca gcctaagcgc aacgggctcg gtcggcgcgt cggggatgtc
     7021 ctgagcccgg tcgagggggc tcgggtccgt cggatcccgg gcggtcgaat cccgttggat
     7081 ggaccacgtg tccgggtgca gactcttcga cgacatcccg ggtgaaggga gcgacggtga
     7141 acgaggactc ggagtacgag caggctcatc aggaggcgag ggtagcgacc gaaggggcgc
     7201 tggcgagcgc ggaccgggcg atggccgatg cggaccggct gaaggctgag gcgctcaccg
     7261 gcgagatgct gcacgaagga ctcaggcagc aggggctgat ctacggcggc ctgatcggga
     7321 tcgccgtggt catggtgcag ccgttcctca tcgcgacgtc tctcgacacc tcggccacga
     7381 tctgcatcgt cgcgttctcg atcgccatcc ccttcctggc ggcgctcgtg atggtcaacc
     7441 ggcaggaggt cttccgtggc cgccggaccc cgtccgtcac ggtcacagtc acgcaggcga
     7501 tcgcccagct ggcggctttc gtcgggatcg tcgccggctt ctggcacatc agctgggtcg
     7561 ccggcgtggt gatgctggtc gccgcgctcg tggcgatggg ggtccactcg gccggcttcg
     7621 tgcgcctgga agtgacgcga aagcccacgc cctaagaggg catccatcgt ccgcggcggc
     7681 accacggtcg tgagcggtcg aaggacgacc cggcgtgtcg agaaccgccc gggcgctccg
     7741 acctatcgat gagagcaccc cggtgaccga atccaggtcg tgcacggaag gaggaggaga
     7801 ctcatggcag acgacacgca ggcacagctc ccgacgcccg atcccgccct tcggcgcctg
     7861 gatcgcttcg tcggaacgtg gagcatggag ggccacctgg tcggctcgga cgagatcacg
     7921 gtcaggggtg agacgacgtt ccgctggctg ccgggaggct tcttcctcga acagcacgcg
     7981 gccctgaact tcctggggct cgagatcgag tcgttggagc tgatcggcta cgacccggag
     8041 accgggacgt tcccctcgac cgtcttctcg aacctgtcgc cgacgccgct gccgtaccgg
     8101 tgggaggtgg ac
//
LOCUS       DATBRK010000151         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_741589, whole genome shotgun sequence.
ACCESSION   DATBRK010000151 DATBRK010000000
VERSION     DATBRK010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741589"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VIH70_04635"
     CDS             <1..919
                     /locus_tag="VIH70_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016823039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEY8115680.1"
                     /translation="IVEDNVNSFPALLTHGAPWVRIMSCNPLELKDPALPPTFSGYPT
                     DDRTGWDEFGAEVERTHRDTWERFNAFMVDSGAPPLPDLEFIHESERLNLYVFPEAAD
                     YRRSHAPAPTWHRLESSVRRTEEAWDVPEELAGDGALVYLSLGSLGSADVELMRRLVA
                     VLADTPHRYVVSKGPRADEFELADNMWGEARVPQTNVIPRCDLVITHGGNNTTTEAFH
                     FGKPMIVLPLFWDQYDNAQRVDELGLGVRLDTYAFEDDELRRGVDRLVGDPDLRSRAE
                     GLGAAIRSADGTERAADLIEAAARDGSAR"
     gene            complement(949..1767)
                     /locus_tag="VIH70_04640"
     CDS             complement(949..1767)
                     /locus_tag="VIH70_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8115681.1"
                     /translation="MSQATVAIPIPSERRGLDRLTGAVSDSFAMTWRNVITLRRVPQL
                     LVFSTVQPVIFVLLFVYVFGGAVQKTFGLPYVDYLIPGIFVQMSVFGAMGTAVGLAAD
                     VKSGLLERFQALPMARSAVLAGRTSADVVRNAFVMTLVSLVGFLVGFRVHTDALGYLG
                     GVVLVLLFGYSLSWVFATVGLAVKDPETAQAAAFPIMAPLVFASSVFVPVESMPSWLQ
                     PFAEHQPVSVVASAARSLMSGTPSDHYVVQALAWIAAIVAISAPLAVRRYRRVF"
     gene            complement(1764..2759)
                     /locus_tag="VIH70_04645"
     CDS             complement(1764..2759)
                     /locus_tag="VIH70_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8115682.1"
                     /translation="MSNANDPAIAVRGLQKRFGDLEALAGIDFEVAPATVFGLLGPNG
                     AGKTTAVRILTTILEPDGGTAEVLGFDVVRDAERVRLRIGLAGQAATVDPNLTGRENL
                     RLVGRLAQRPRAWVDLRAAELLELFELSPAADRPIRTYSGGMRRRLDVAAALVGRPPV
                     LFLDEPTTGLDLPSRNALWRTIRDLVADGTTVLLTTQYLEEADQLAARVAVVDVGRVI
                     AEGTPSELKAQLGSTVIEFVMAEELDAHRAASALASPRGTPEVEGARIRVPSGEGPRA
                     LMEMLRTLDAQGLYPVSLTVREPSLDDVFLRLTGHHAEPAPELESAEPVPVGGGA"
     gene            2885..3334
                     /locus_tag="VIH70_04650"
     CDS             2885..3334
                     /locus_tag="VIH70_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012757990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8115683.1"
                     /translation="MDVRTAGTSKRTLAKQAWKPLARFFFETAKHRQQVFADSGLTPN
                     DARALFVLDRTRGRSMSELADEWLCDASNATWIVDRLEERGLAERRTIPTDRRVKLVV
                     LTAKGDRTRNEMIRTLYEPPPELLELDLAELEALRDAVARLPLNGAS"
     gene            3607..>3791
                     /locus_tag="VIH70_04655"
     CDS             3607..>3791
                     /locus_tag="VIH70_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115684.1"
                     /translation="MGTTIAHAEAIGTRRGSRLAIAAHGVNHPTYWREKTGEHASSAH
                     TATSDGTPTRSSRSDLSA"
BASE COUNT          632 a         1350 c         1314 g          495 t
ORIGIN      
        1 catcgtcgag gacaacgtca acagcttccc ggcgctgctc acccacggcg cgccgtgggt
       61 gaggatcatg tcgtgcaacc cgctcgagct gaaggacccc gcgctcccac cgacgttcag
      121 cggctacccc acggacgacc gaacgggatg ggacgagttc ggcgccgagg tcgaacgcac
      181 ccaccgcgac acctgggagc ggttcaacgc gttcatggtg gacagcgggg cgccgccgct
      241 ccccgacctc gagttcatcc acgagtccga gcgcctcaac ctctacgtct tccccgaggc
      301 ggccgactac cgtcggtcgc acgcgccagc ccccacctgg catcggctgg agtcgtccgt
      361 ccggcggacc gaggaggcgt gggacgtccc cgaggagctc gcgggtgacg gcgcgctcgt
      421 gtacctgtcg ctcgggtcgc tcgggtcggc ggacgttgag ctgatgcggc ggctcgtcgc
      481 ggtgctggcg gacaccccgc accggtacgt cgtgtcgaag ggtccgcgcg ccgacgagtt
      541 cgagctggcg gacaacatgt ggggagaggc ccgagttccc cagaccaacg tgatcccgcg
      601 ctgcgatctg gtgatcaccc acggcggcaa caacacgacg accgaggcgt tccacttcgg
      661 caagccgatg atcgtcctgc cgctgttctg ggaccagtac gacaacgccc agcgggtgga
      721 cgagctcggc ctgggcgttc gcctcgacac atacgcgttc gaggacgacg agctgcgacg
      781 cggggtggac cgcctggtgg gcgacccgga cctgcgttcg agggcggagg gcctgggggc
      841 cgcgatccgg tcggccgacg ggaccgagcg cgccgcggac ctgatcgagg ccgcggcgcg
      901 ggacggctcg gcccgctaac ccgcctcctg atccggcgct cgggccgatc agaacacgcg
      961 tcggtaccgg cgcaccgcca ggggtgccga gatcgcgacg atcgcggcga tccacgcgag
     1021 cgcctgcacc acgtagtgat cgctcggcgt gccgctcatc aatgagcgag ccgccgaggc
     1081 caccaccgag accggctggt gctcggcgaa gggctggagc cagctcggca tcgactccac
     1141 cggcacgaac accgacgagg cgaacaccag cggggccatg atcgggaacg cggccgcctg
     1201 ggcggtctcg ggatccttca cggcgagccc cacggtcgcg aagacccacg acagcgagta
     1261 gccgaacagc agaaccagca cgacgccgcc caggtagccg agggcgtccg tgtgcacgcg
     1321 gaacccgacc aggaagccga ccaggctcac gagcgtcatg acgaaggcgt tgcgcacgac
     1381 gtcggccgag gtcctgccgg ccagcaccgc cgatcgcgcc atcggcagcg cctggaaccg
     1441 ttcgagcagt ccgctcttca cgtcggcggc gagcccgacc gccgtcccca tcgcgccgaa
     1501 caccgacatc tggacgaaga tgcccgggat caggtagtcg acgtagggca gaccgaacgt
     1561 cttctgcacc gccccgccga acacgtagac gaacagcagc acgaagatga ccggctggac
     1621 cgtggagaac accaggagct gcggcacgcg gcggagcgtg atcacgttgc gccaggtcat
     1681 ggcgaaggag tcggagacgg ccccggtcaa cctgtcgagg ccgcggcgct cggacgggat
     1741 cgggatcgcg acggttgcct ggctcatgct ccacctccga ccggaacggg ctcagccgac
     1801 tcgagctccg gggccggctc ggcgtggtgt ccggtcagtc gcaggaagac gtcgtccagg
     1861 ctcggctcgc gcacggtgag cgacacggga tacagcccct gcgcatcgag ggtcctgagc
     1921 atctccatca gcgcccgggg accctcgccc gagggcaccc ggatcctcgc gccctcgacc
     1981 tccggcgtac cccgcggtga ggccagggcc gacgccgcgc ggtgcgcgtc gagctcctcc
     2041 gccatcacga actcgatcac ggtgctcccg agctgagcct tgagctcgga cggcgtcccc
     2101 tcggcgatca cgcgcccgac gtcgaccacc gcgacccgtg cggcgagctg atccgcctcc
     2161 tcgaggtact gcgtcgtcag cagcacggtc gtgccgtccg cgacgaggtc ccggatcgtg
     2221 cgccagagcg cgttccggct cgggaggtcc aggcccgtcg tgggctcgtc gaggaacagg
     2281 accgggggtc gtccgacgag ggcggcggcc acatcgagcc ggcgccgcat cccaccggag
     2341 taggtcctga tcggccggtc ggcggccggc gagagctcga acagctccag gagctcggcc
     2401 gcccggaggt cgacccacgc gcgcggtcgt tgcgcgagcc gcccgaccag ccggaggttc
     2461 tcgcggccgg tgaggttggg gtccaccgtg gcggcctgcc cggccagccc gatgcgcagc
     2521 cggacgcgtt cggcgtcgcg cacgacgtcg aacccgagga cctccgcggt gcccccgtcg
     2581 ggctcaagga tcgtcgtgag gatgcggacg gcggtcgtct tgccggcgcc gttggggccg
     2641 aggagcccga acacggtcgc gggggccacc tcgaagtcga tcccggcgag agcctccagg
     2701 tcgccgaacc gcttctgcag cccacgcacc gcgatcgcgg ggtcgttcgc gttcgacatc
     2761 ggcacctcca tgttcgcgga ccggaccagg atgccacaga tagttgagtg atgacaagta
     2821 tccaggaatc tggaagattg aacgtattgg accttcaggg cttcgtcggg taggattgct
     2881 ctctatggac gtccgcaccg cgggcacgag caagcgaacc ctcgccaaac aggcctggaa
     2941 gccgctcgcc cgattcttct tcgagacggc gaagcaccgc caacaggtgt tcgccgacag
     3001 cgggctgacg cccaacgacg cgcgggcgct cttcgtcctc gaccgcacgc gcggccgttc
     3061 gatgagcgag ctcgccgacg aatggctgtg cgacgcatcg aacgcgacgt ggatcgtcga
     3121 ccggctcgag gagcgaggcc tcgcggagcg acgaacgatc cccaccgacc gccgggtcaa
     3181 gctggtggtc ctcaccgcga agggcgacag gaccaggaac gagatgatcc ggaccctcta
     3241 cgagccgccg cccgagctgc tcgagctcga tctcgcggag ctcgaggccc ttcgcgacgc
     3301 cgttgccagg ctccccctca acggcgcctc ctgaccggcg gatcgctggg gagcgatcgg
     3361 gcgagtcgcc tggtcgggct agcctcgcgc tccgaacgcc gacttcccgg caggccgatc
     3421 ggcacgaacg agtcgtgcct ccgcggagct gctctggctc gcccctggca tggccaccgc
     3481 gggccggcca cgcgtcaggt accagatcgg gaggaagaga cacgatccgt tcctcgccag
     3541 cagcatgacc gccccgagat ccccaagctg tcggcgtact tccagagcgc gacggtcact
     3601 cccgcggtgg gcacgacgat cgcccacgcc gaggcgatcg gaacgcggcg cgggtcacga
     3661 ctcgcgatcg ccgcccacgg tgtcaaccat ccgacgtatt ggagggagaa gacgggggaa
     3721 cacgcaagca gcgcgcacac cgcgacgagc gacgggaccc cgacgaggtc ctcccgttcc
     3781 gacctgagtg c
//
LOCUS       DATBRK010000152         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2478896, whole genome shotgun sequence.
ACCESSION   DATBRK010000152 DATBRK010000000
VERSION     DATBRK010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2478896"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VIH70_04660"
     CDS             complement(<1..1140)
                     /locus_tag="VIH70_04660"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8115685.1"
                     /translation="MKTLYRASLVHTLSYPAMGEWVLVDGRHVERVGVGEVPHADRTV
                     DLPGTTILPGFVDTHVHLTGTGVHHQAPELGSVASASGLLDVARSVAAARQGPVYLHG
                     WDESGWRDRTLPTLAELDGVTDRPLALVRVDGHLLLANSPALEAIQAASEPGVETDDD
                     GAPTGRLTAKAGQAAKRWFATNLSEHDVEELQLEAASLAVAFGVTTIHEMSMPAERGM
                     RDLEILLAHRSRLPLEVVHYVATTDISTAMNLGLPRIGGDLPVDGSIGARTAFVSGGF
                     VDTDSSPSGYFEDDELAQYFHDGHLAGLQVGVHAIGDAAVEQVVRTWERVYLSLDSRQ
                     RRHFRARRHRIEHFEMSSLDVIERAAILGLAISVQPTFDARWGFPG"
     gene            complement(1188..>1589)
                     /locus_tag="VIH70_04665"
     CDS             complement(1188..>1589)
                     /locus_tag="VIH70_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEY8115686.1"
                     /translation="IAFADVRRCYASMSPSIVGDALGRAGIPTACEVEGFLAGLERIG
                     VEGLPVGPDASAVLANAVLAQVDRTLREAGIEHLRWVDDVVLSGGDGPAALSVFRAAL
                     ARIGLRANEAKTRILPDARGLGSTATVSGGS"
BASE COUNT          232 a          577 c          568 g          212 t
ORIGIN      
        1 gcccgggaac ccccaacggg cgtcgaaggt cggctgcacg ctgatcgcga ggccgaggat
       61 cgccgcgcgt tcgatcacgt ccaggctcga catctcgaag tgctcgatgc ggtgacgccg
      121 tgcccggaag tgccggcgct ggcgcgagtc gagcgacagg tacacgcgct cccaggtccg
      181 cacgacctgc tcgacggcgg cgtccccgat cgcgtgcacg ccgacctgca ggcccgcgag
      241 gtgaccgtca tggaagtact gcgcgagctc gtcgtcctcg aagtagccgc tcgggctcga
      301 gtcagtgtcc acgaatcccc cggagacgaa cgctgtccgc gcgccgatcg acccgtcgac
      361 ggggaggtcc ccgccgatcc gcggcaggcc gaggttcatc gcggtcgaga tatcggtcgt
      421 cgcgacgtag tggacgacct cgagcggcag ccgggagcgg tgcgcgagga ggatctcgag
      481 atcgcgcatc ccgcgttcgg cgggcatcga catctcgtgg atcgtcgtga cgccgaaggc
      541 gacggccagg ctcgcagcct cgagctgcag ctcctcgacg tcgtgttcgg acaggttcgt
      601 ggcgaaccac cgcttggcgg cctgtccggc cttcgcggtg agccggccgg tcggcgcgcc
      661 gtcgtcgtcg gtctcgaccc cgggctcgct tgccgcctgg atcgcttcca gggccgggga
      721 gttggcaagg agcaggtgcc cgtccacgcg gacgagcgcc agcggccgat cggtgacgcc
      781 gtcgagctcg gccagcgtcg gcagggtccg gtcccgccag ccggattcgt cccagccgtg
      841 caggtacacg gggccctgcc tcgccgccgc gaccgaccgt gcgacgtcga gaagcccgga
      901 ggccgaggcc accgatccga gctcgggcgc ctggtggtgc acaccggtgc cggtcaggtg
      961 gacgtgcgta tcgacgaacc ccggcaggat cgtggtcccc ggcaggtcga cggtccggtc
     1021 ggcgtgggga acctcgccga cgccgacgcg ttcgacgtgg cgcccgtcga ccaggaccca
     1081 ctcccccatc gccggatacg acagcgtgtg gacgagcgaa gcgcggtaca gggtcttcat
     1141 cgccgggcga gcttagcccc aagcggccgg ccggcgtttc ggacacctta cgaaccgccg
     1201 gacacggtgg cggtcgatcc gaggcctcgg gcgtccggca ggatcctcgt cttggcctcg
     1261 ttcgcccgga gcccgatcct cgcgagggcc gcccggaaca cggacagggc ggccgggccg
     1321 tccccaccgg aaaggacgac gtcgtccacc catcgcaggt gctcgatccc ggcctcccgc
     1381 agggttcggt ccacctgcgc gaggacggcg ttggccagga ccgccgacgc gtccgggccg
     1441 accggcaaac cctcgacgcc gatccgctcg aggcccgcga ggaacccctc gacctcgcac
     1501 gccgtgggga tccccgcccg accgagcgcg tcgccgacga tcgacggcga catcgacgcg
     1561 tagcaccgcc ggacgtcggc gaacgcgat
//
LOCUS       DATBRK010000153         8299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1320404, whole genome shotgun sequence.
ACCESSION   DATBRK010000153 DATBRK010000000
VERSION     DATBRK010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320404"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VIH70_04670"
     CDS             <1..1170
                     /locus_tag="VIH70_04670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8115687.1"
                     /translation="PIKHQHWATVPWHTMGVYHLGNNLEFHGDIYDLAIRTPGLLVIH
                     DLALDDFVMGMVATAQPFGHQALREGLLLAPRLHGFEEAERNEPLRVPYVAHAARHAR
                     GIIVHSPFVERYLRVFGCKTPIYVAPHPVIEKEQDVRRAEGRARVIRGSLESMGMRTL
                     IGVFGDQNAAKMIDVVLEAMVHLPQDVHLALVGRRIPGYDTEPLVRASGLGARVSLHT
                     DVSDEDFLAWMCAADVAVDLRYPHRGEVSGSLSRSMQCGRPTVVSATGTYLDLPEDVV
                     VRVPAGRLEPRELAEALRALVDDPERRRRIGDAARTRSAELAASEATARVYAEAMDAT
                     MALLWDPARRALARWGGALVDLGITEEGLSEGYGMAYARALDEFRPSGSADSSST"
     gene            complement(1110..2258)
                     /locus_tag="VIH70_04675"
     CDS             complement(1110..2258)
                     /locus_tag="VIH70_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8115688.1"
                     /translation="MAIASTVRKRWVEIAWAAFAAANVVVIVMLRDWETIPFHFIWVS
                     LTLVYGLRVWRLRTTMLLLLVVMAVTGAALAWTVVRGNERLDELTEVPLMAAMFVAIV
                     WHAHRRQAAIEETSRLAESEHRLLERQREFVRDASHELRTPITVARGHAELIRHAGGD
                     GQVAIDADVVLDELEHLSSLSERLLILTAAEDPAFLSRTDIEVEPLVVGLMRRWGPTA
                     ERTWQVRVGVDGTILADRERLETALDALMENAVEATDEASCIRVGCRGEVDTLVLEVT
                     DQGRGICAEDLPRIFDPFSKIEPDRARLNGGTGLGLSIAKAIVDAHGGSIAVESAEGR
                     GTTFRIRLPGLRPSWPDRSATSAIASDIDQVEEESAEPEGRNSSSARA"
     gene            complement(2233..2898)
                     /locus_tag="VIH70_04680"
     CDS             complement(2233..2898)
                     /locus_tag="VIH70_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8115689.1"
                     /translation="MSSVLVVDDEPMIVSFVARALSAEGFHVDSATDGTRGLELAGTG
                     IYELVVLDLLLPSLDGESVLRRLIESRPTQRVLILSALTDVDTKVRCLEFGASDYLSK
                     PFSLAELVARIRARLRQPGSGPEQRLLQARGVTLDLTRRVADSGGGQVSLSEREFLLL
                     EHLMRRDGEVCTRQRLLADVWGYSFDPGSNMVDVCVGRLRAKLGTDVIETVRSVGYRF
                     NGS"
     gene            3099..3521
                     /locus_tag="VIH70_04685"
     CDS             3099..3521
                     /locus_tag="VIH70_04685"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HEY8115690.1"
                     /translation="MTRRVRRWLIVPMILGLALVTAGCSGDDEGVGVTLADFSVTPDP
                     DSAPAGETTFDVTNDAEQTHEFVVFKTDLAEDQLPTDENGDVDESGEGVELVNEIEDI
                     EGGSSESLTVNLDAGSYVFICNLPGHYKQGMHASFTAS"
     gene            3727..4632
                     /locus_tag="VIH70_04690"
     CDS             3727..4632
                     /locus_tag="VIH70_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8115691.1"
                     /translation="MADTIQTVERTSESPRLRVGERLRILSAHREILANLVRKELKVK
                     YAASVLGAVWSLLNPLVYLAVFSFVAKVLGAGIPNYPVYLLSGLLAWNLFSASMGSGA
                     RAVLDNANLVKKVAFAREILPLSAVGVGLVDFALQSAVLLLYIIVSGYGLHLPELALW
                     PLAFVTLLMLTVTLSLWFSALNVRYRDVGHLLNIALLVWFWATPIVYAEFQVQQLAEK
                     QTWFGIQRLAFYLLNPMVDIVAGFHRAMYGTVTPIGAPEPILFDKSLGWVAGILTITL
                     VASLVGLRLAWGYFFNRSGDFAEEL"
     gene            4629..5810
                     /locus_tag="VIH70_04695"
     CDS             4629..5810
                     /locus_tag="VIH70_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8115692.1"
                     /translation="MNAAAIEVADVSKRFRIYRDKPTSLKQRVLSSRSRAEDFWALRD
                     VALDVGQGSTFGLIGHNGSGKTTLLKCVAGILRPTSGTIRQRGRLAALLELGAGFHPE
                     LTGRENVYLNASFLGLSRRQTDAAFDDIVAFAELEDFIDNEVKFYSSGMLVRLGFAVA
                     VHVEPDVLLIDEVLAVGDEAFQAKCLDRVRAFQREGRTIVLVTHALDTVIEICDRAAM
                     LHHGELHAIGMPADVVREMRYVLLGVTDPNFVPEEGTREAEIAEVQIVRPNGSSEGAI
                     LRGDPLTIVIDVRQNEPLDDLDVDFAVLDGATNHAVLDARTSRADLDLGRFEKKRVRF
                     QIEAFPYEPGKYWVTVGLSNRTTGHLYHVQTQRYLFEVVDAPRVQERVEVPVAVEIED
                     L"
     gene            5807..6238
                     /locus_tag="VIH70_04700"
     CDS             5807..6238
                     /locus_tag="VIH70_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115693.1"
                     /translation="MTEPATDAITTQTKGTPPMLIVLILFSVTLAAVAQLALKHGMNL
                     VNDELAPQTFSLNGSSLRVVVGQPFVWSGLFLFGLSALVWLVVLSRASLSFAYPFAAL
                     TYVLILLFDQFVLDETVPPLRWAGVAFIGVGIILVSQTQHT"
     gene            6243..7265
                     /locus_tag="VIH70_04705"
     CDS             6243..7265
                     /locus_tag="VIH70_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY8115694.1"
                     /translation="MKAVVLVGGEGTRLRPITETVPKPLVPLVDRPSLDHVLDHLAGH
                     GVHEVVLSSSYLEETFHAFIQARRGDPSITWITETEPLGTGGAIVNALGALDTHDPYF
                     ALNGDILTDLDLTDMLARHRDRSAVATIALTHVEDARPYGLVPIDADGRVLEFREKPS
                     VRMPGDINAGTYVLDPAALEGWTHGENVSIERQIFPALIAAGRPLAGFPSDAYWMDLG
                     TPQAYLQAQFDLLDGRVDGHRSYPSPFVDTGGRIDRGATVSQHVVVGPRATIETGARV
                     SDTVLHERAVIEGDTVVERSILGPGAMVMRGASVRDSVLGEGAVVDAGASVSEGRVQP
                     GERVEA"
     gene            7338..>8299
                     /locus_tag="VIH70_04710"
     CDS             7338..>8299
                     /locus_tag="VIH70_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY8115695.1"
                     /translation="MRRVLILALLAALAIPWVTGATPAVAGSQRAPDGPSRFKMFGAG
                     FGHGLGLSQWGAYGLARQGWGPSKILKHFYSGTRVARETNPPGNLRIGLVQGKDSIRL
                     EAQVGSIEIRLGDPQTGSTVATIPAGETWHVRAASNEYLIVDQAGGTVDRVGGPNEPI
                     YLVYESAQSRVRIPEAGHAYNRGSIEFALYSCAGGCAMRLVLTIDPRGYLYGLGEVPS
                     SWPMSALKTQAIAARTYAFTKAAASQHRAVCDCALYASSYDQVYAGWDKEGGIDGDRW
                     VSAVNQTGDQVVLNGGQTIQAFYMSSSGGYTENNENVWGGTPID"
BASE COUNT         1275 a         2893 c         2831 g         1300 t
ORIGIN      
        1 ccgatcaagc accagcactg ggcgacggtg ccgtggcaca cgatgggcgt ctaccacctc
       61 ggcaacaacc tggagttcca cggcgacatc tacgacctcg cgatccggac gcccggactc
      121 ctggtgatcc acgacctcgc gctcgacgac ttcgtgatgg ggatggtggc cacggcgcag
      181 ccgttcgggc accaggcgct gcgcgagggt ctgctgctcg cccctcggct ccacgggttc
      241 gaggaggccg aacgcaacga gccgctccgc gtcccgtacg tcgcgcacgc cgcccgtcac
      301 gcgcgcggca tcatcgtgca ctcgccgttc gtcgaacgct acctgcgcgt gttcggctgc
      361 aagaccccga tctacgtcgc cccgcacccc gtcatcgaga aggagcagga cgtccgcagg
      421 gccgaggggc gcgcgcgtgt gatccgcgga tcgctcgagt cgatggggat gcggacgctg
      481 atcggcgtgt tcggcgacca gaacgccgcc aagatgatcg acgtcgtcct cgaggcgatg
      541 gtgcacctgc cccaggacgt ccacctcgcg ctcgtcggac gacggatccc cgggtacgac
      601 acggagccgc tggtccgagc gagcggtctg ggggcccgcg tgtcgctgca caccgacgtc
      661 tcggacgagg acttcctcgc gtggatgtgc gccgcggacg tggcggtgga cctccggtat
      721 ccgcaccgcg gggaggtgtc cgggtcgctc tcgcgctcga tgcagtgcgg gcgccccacg
      781 gtcgtcagcg cgacggggac ctacctcgac ctccccgagg acgttgtcgt ccgcgtgccg
      841 gcggggcggc tcgagccgcg ggagctggcc gaggcgctcc gagcgctcgt cgacgatccc
      901 gaacgccgcc gccggatcgg cgacgcggcg cgaacgcgct cggccgagct cgcggcgtcc
      961 gaggcgaccg cccgcgtcta cgccgaggcg atggacgcga cgatggcgct cctgtgggat
     1021 ccggcccggc gcgcgctcgc ccgttggggc ggggcgctgg tggacctcgg gatcaccgag
     1081 gaggggctgt cggagggcta cgggatggcc tacgcccgag cgctcgacga gttccgaccc
     1141 tccggctcgg ccgactcctc ctcgacctga tcgatatcgg acgcgatcgc actcgtcgcc
     1201 gaccgatcag gccacgacgg ccggagcccc ggcaacctga tccggaaggt cgtaccacga
     1261 ccctccgcgc tctccacggc gatcgaccca ccgtgggcat cgacgatcgc cttcgcgatg
     1321 gaaaggccga gtcccgtccc gccgttcagc ctggcccggt ccggctcgat cttcgagaat
     1381 ggatcgaaga tccgcgggag gtcctcggcg cagatgcccc tgccctggtc cgtgacctcg
     1441 agcacgagcg tgtctacttc cccccgacat ccgacgcgga tgcagctcgc ctcgtcggtc
     1501 gcctcgacgg cgttctccat caatgcgtcg agcgcggtct ccaaccgttc gcgatcggcc
     1561 aagatcgttc catcgacccc gacccgaacc tgccaggtcc gctccgccgt gggcccccat
     1621 cggcgcatca gcccgacgac gaggggctcg acctcgatgt cggtcctgga gaggaaggcc
     1681 ggatcctccg ccgcggtcag gatcagcagg cgctcggaga ggctcgagag gtgctcgagc
     1741 tcgtcgagga cgacatcggc gtcgatcgcg acctgtccgt ccccaccggc atgccggatg
     1801 agctcggcat gaccgcgggc gaccgtgatc ggcgtccgga gctcgtgcga ggcgtcgcga
     1861 acgaactctc gctgccgttc gaggagccga tgctcggact ccgccagcct gctggtctcc
     1921 tcgatggccg cttgtctccg atgggcatgc cacacgatcg ccacgaacat cgccgccatc
     1981 aacgggacct cggtcagctc gtcgagtcgc tcgttgccgc gcaccacggt ccaggcgagc
     2041 gccgcaccgg tgacggccat caccacgagc aggaggagca tcgtcgtccg cagccgccac
     2101 acccgcagtc cgtagacgag cgtgaggctc acccagatga agtggaaggg gatcgtctcc
     2161 cagtcccgaa gcatcacgat cacgaccacg ttggcggcgg caaaggccgc ccacgcgatc
     2221 tcgacccagc gtttacgaac cgttgaagcg atagccaaca cttcgcaccg tctcgatcac
     2281 atcggtcccc agcttcgccc ggagacgccc gacgcagacg tccaccatgt tgctgccggg
     2341 atcgaacgag tacccccaca cgtcggcgag taatcgctgc cgcgtgcaga cctccccgtc
     2401 cctccgcatc aggtgctcga ggagaaggaa ctcgcgttcg ctgagggaca cctggccgcc
     2461 gccggagtcc gcgacgcgcc gcgtgagatc cagggtcacg ccgcgtgcct ggagcaggcg
     2521 ctgctcggga ccggacccgg gctggcggag acgcgccctg atccgcgcca ccagttcggc
     2581 cagcgagaac ggcttggaca ggtagtcgga cgcgccgaac tcgaggcacc gtaccttggt
     2641 gtcgacgtcg gtcagggccg acaggatcag aacccgctgg gtcggtcggg attcgatgag
     2701 gcgccggagc accgactcgc cgtcgaggct cggcaggagc agatcgagca cgacgagctc
     2761 gtagatgccg gtgccggcta actcgaggcc acgggtcccg tctgtcgcgc tgtccacgtg
     2821 gaacccctcg gcagagaggg ctcgggccac gaagctgacg atcatcggtt cgtcgtcgac
     2881 cacgaggacg ctcgacacac ctcgaatgct attggcgaac gcgccacccg gccatccttc
     2941 gtgacgagac cgtcatccgg ccaggcaggg atcgtgagcg gagcgtgacg gttgcatgac
     3001 gtcagcgtca tggacgcccg gtcttcgcaa aggagtgcct acagtcgcgg acggtgaccg
     3061 gtctcaccca gacctggacc ggccgaaggg aggcctccgt gacccgacgc gtgcgacggt
     3121 ggttgatcgt tccgatgatc ctggggctcg ccctcgtgac ggcgggctgc agcggcgacg
     3181 acgagggcgt cggagtgacg ctcgccgatt tctcggtcac ccccgacccg gacagcgcgc
     3241 ctgcgggcga gaccaccttc gacgtcacca acgacgccga gcagacccac gagttcgtcg
     3301 tcttcaagac cgatctcgcc gaagatcagc tcccgacgga cgagaacggc gacgtcgatg
     3361 agtccggcga gggcgtggag ctcgtgaacg agatcgagga catcgagggt ggaagcagcg
     3421 agtcgctcac ggtgaacctg gacgcgggca gctacgtttt catctgcaat ctccccggcc
     3481 actacaagca ggggatgcac gcgagcttca ccgccagcta gccgaccgtc gaggggtccc
     3541 tcgcccggcc cggcaggccg agaccgggcg ggctgggcgg gccagcggcg tcgtgcgaac
     3601 ggcgcccgtg ctcccgggat gacgattccg tgcgttctcc acgggtgccg cgaaacgcgt
     3661 tgctagactc gccacgccgg ccggcacgca cgtcacggat ccgaaccggc ctgccgccca
     3721 acgcctatgg ccgataccat ccagaccgtc gagcgcacct ccgagtcgcc gcgcctccgg
     3781 gtcggcgaac gcctccgcat cctctccgcc caccgcgaga tcctggcgaa cctggtccgc
     3841 aaggagctca aggtaaagta cgcggcctcg gtgctcgggg ccgtgtggtc gctgctgaac
     3901 ccgctcgtct acctcgcggt cttctcgttc gtggcgaagg tcctgggcgc cgggatcccc
     3961 aattaccccg tctacctgct gtccggactc ctggcctgga acctgttctc ggcctcgatg
     4021 ggcagcggcg cgcgggcggt gctcgacaac gcgaacctcg tgaagaaggt ggcgttcgcg
     4081 cgcgagatcc tgccgctgtc ggccgtcggt gtcggcctgg tcgacttcgc gttgcagtcc
     4141 gcggtcctgc tcctctacat catcgtgtcg ggctacggcc tgcacctgcc cgagctcgcg
     4201 ctgtggccgc tcgcgttcgt gacgctcctg atgctcacgg tgaccctgtc gctgtggttc
     4261 tcggcgctga acgtgcgata ccgcgacgtc gggcacctgc tcaacatcgc gctgctggtg
     4321 tggttctggg cgaccccgat cgtctacgcc gagttccagg tgcagcagct cgccgagaag
     4381 cagacgtggt tcgggatcca gcgactcgcc ttctacctgc tcaacccgat ggtcgacatc
     4441 gtggccggct tccaccgagc gatgtacggc acggtgaccc cgatcggcgc gcccgaaccc
     4501 atcctgttcg acaagagcct cggctgggtc gccgggatcc tcacgatcac gctcgtcgcc
     4561 tcgctcgtcg ggctccggct cgcgtggggc tacttcttca accgttcggg cgacttcgcg
     4621 gaggagctgt gaacgccgcc gcgatcgagg tcgccgacgt ctcgaagcgc ttccgcatct
     4681 atcgggacaa gcccacgtcg ctcaagcaac gcgtgctctc ctcgcggagc cgagccgagg
     4741 acttctgggc gcttcgcgac gtggccctcg acgtgggcca ggggtcgacg ttcgggctga
     4801 tcgggcacaa cgggtcgggc aagacgacgc tcctgaagtg cgtcgccggg atcctgcggc
     4861 cgacctcggg gacgatccgc cagcgcgggc ggctcgcggc gctgctcgag ctcggcgccg
     4921 ggttccaccc cgagctcacg gggcgcgaga acgtctacct gaacgcctcg ttcctggggt
     4981 tgtcccgcag gcagaccgac gctgcgttcg acgacatcgt cgcgttcgcc gagctcgagg
     5041 acttcatcga caacgaggtg aagttctact cgtcgggcat gttggtccgg ctcgggttcg
     5101 cggtggcggt gcacgtcgaa cccgacgtcc tgctgatcga cgaggtgctc gcggtcggcg
     5161 acgaggcctt ccaggccaag tgcctcgacc gcgtccgagc cttccagcgc gaaggacgca
     5221 cgatcgtgct cgtgacgcac gcgctcgaca ccgtgatcga gatctgcgac cgggcggcga
     5281 tgcttcacca cggcgagctg cacgcgatcg gcatgccggc cgacgtcgtg cgggagatgc
     5341 ggtacgtcct gctcggcgtg accgatccga acttcgtccc cgaggagggc acccgcgagg
     5401 ccgagatcgc ggaggtccag atcgtccggc cgaacggctc gagcgagggc gcgatcctgc
     5461 gcggcgaccc gctcacgatc gtgatcgacg tccgccagaa cgagccgctc gacgacctgg
     5521 acgtggactt cgcggtcctg gacggggcga cgaaccatgc ggtgctcgac gctcggacct
     5581 cgcgcgccga tctggatctc gggcggttcg agaagaagcg cgtgcgcttc cagatcgagg
     5641 ccttcccgta cgagcccggg aagtactggg tgacggtcgg gttgtccaac cgcaccaccg
     5701 ggcatctgta ccacgtgcag acccagcggt acctgttcga ggtcgtcgac gcgcctcgtg
     5761 tgcaggagcg cgtcgaggtt ccggtggccg tggagatcga agacctgtga ccgaacccgc
     5821 gaccgacgcg atcacgaccc agacgaaagg aacccctccg atgttgatcg tgctgatcct
     5881 gttctccgtg acgctggccg cggtggcgca gctggcgctc aagcacggca tgaacctcgt
     5941 gaacgacgag ctcgcgccgc agaccttctc gctgaacggt tcctcgctgc gggtggtcgt
     6001 cggccagccg ttcgtctgga gcgggttgtt cctgttcggc ctgtccgcgc tggtctggct
     6061 ggtggtgctg tcgagggcct cgctctcgtt cgcctacccg ttcgccgcgc tcacgtacgt
     6121 gctgatcctg ctcttcgacc agttcgtgct tgacgagacg gtgccgccgc tgcgctgggc
     6181 gggggtcgcg ttcatcggtg tggggatcat cctcgtgtcg cagacccagc acacgtgacc
     6241 cgatgaaggc ggtcgtgctg gtcggcggtg agggcacacg cctgcgcccg atcaccgaga
     6301 ccgtgcccaa gcctctcgtc ccgctcgtgg accggccgag cctggatcac gtgctcgacc
     6361 acctcgcggg ccacggcgtg cacgaggtcg tgctgtcgag ctcgtacctg gaggagacct
     6421 tccacgcgtt catccaggcc cgtcgcggcg acccgtcgat cacctggatc accgagaccg
     6481 agccgctcgg cacgggcggc gcgatcgtga acgcgctcgg agccctcgac acgcacgacc
     6541 cctacttcgc cctcaacggc gacatcctca ccgacctgga cctcaccgac atgcttgcga
     6601 ggcaccgcga tcgcagcgcg gtggcgacga tcgcgctcac ccacgtcgag gacgccaggc
     6661 cttacggcct cgtgccgatc gacgccgacg ggcgggtgct cgagttccgc gagaagccgt
     6721 cggtgcgcat gccgggcgac atcaacgcgg gcacctacgt cttggatcct gccgcgctcg
     6781 agggttggac ccacggcgag aacgtgtcca tcgagcgcca gatcttcccg gcgctcatcg
     6841 ccgcgggacg gccgctcgcg gggttcccgt ccgacgcgta ctggatggat ctcgggaccc
     6901 cgcaggcgta cctgcaggcg cagttcgacc tgctcgacgg ccgcgtggac ggccaccgct
     6961 cgtacccgtc gcccttcgtc gacaccggcg gccgcatcga ccggggagcc accgtgagcc
     7021 agcacgtcgt cgtcggtccg cgtgcaacga tcgagaccgg ggcacgggtg agcgacaccg
     7081 tcctgcacga gcgggccgtg atcgaaggcg acacggtcgt ggagcgctcg atcctcgggc
     7141 ccggcgcgat ggtgatgcgg ggtgcgtcgg tccgcgactc cgtcctcggc gaaggggcgg
     7201 tggtggacgc cggcgcctcg gtgagcgagg gtcgcgtcca accgggggag agggtcgagg
     7261 cgtaaccatc ggtcccgccg ggacgttgtg atacggtgcg gagccgcccg accacagccg
     7321 atcgaagcct tcggagcatg cgcagagtcc tcatcctcgc cctcctcgcc gcccttgcga
     7381 tcccttgggt taccggcgcg accccggcgg tcgccgggtc gcagcgcgcg cccgacggtc
     7441 cgtcgcggtt caagatgttc ggcgcggggt tcgggcacgg cctggggctc tcgcagtggg
     7501 gcgcgtacgg ccttgcgagg cagggctggg gtccctcgaa gatcctcaag cacttctact
     7561 cgggcacgcg cgtcgcgcga gagaccaacc cgccggggaa ccttcggatc gggctcgtcc
     7621 agggcaagga ctcgatccga ctcgaggcgc aggtcggctc gatcgagatc cggctgggcg
     7681 acccgcagac cggttcgacg gtcgcgacga tccccgccgg ggagacgtgg catgttcgcg
     7741 ccgcgagcaa cgagtacctg atcgtcgacc aggccggcgg cacggtcgat cgcgtcgggg
     7801 ggcccaacga accgatctac ctcgtctacg agtcggcgca atcgcgagtg cgcatccccg
     7861 aggccgggca cgcctacaac cgtgggtcga tcgagttcgc cctgtactcg tgcgcggggg
     7921 gatgcgcgat gcgactcgtc ctcacgatcg atcctcgggg ctacctctac ggcctcggcg
     7981 aggtcccgag ctcgtggccg atgtcggcgc tcaagaccca ggcgatcgcg gcgcgaacgt
     8041 acgcgttcac gaaggccgcc gcctcgcagc accgagcggt gtgcgactgc gcgctgtacg
     8101 cgagctcgta cgaccaggtg tacgcgggtt gggacaagga gggcgggatc gacggtgacc
     8161 gctgggtctc cgcggtgaac cagacgggcg atcaggtggt gctgaacgga gggcagacga
     8221 tccaggcctt ctacatgtcc tcctcgggcg ggtacaccga gaacaacgag aacgtgtggg
     8281 gcggcacgcc gatcgacta
//
LOCUS       DATBRK010000154         6582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1897779, whole genome shotgun sequence.
ACCESSION   DATBRK010000154 DATBRK010000000
VERSION     DATBRK010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897779"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1211
                     /gene="ugpC"
                     /locus_tag="VIH70_04715"
     CDS             9..1211
                     /gene="ugpC"
                     /locus_tag="VIH70_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8115696.1"
                     /translation="MYSDGTTAVSDLSLDIKDEEFIVLVGPSGCGKTTALRMVAGLES
                     ITHGTIAIGDRVVNTVPPKERDIAMVFQNYALYPHMSVYDNMAFGLKLRKLSKEEIDR
                     RVMQAAEILGLEEFLQRKPKALSGGQRQRVAMGRAIVREPQAFLMDEPLSNLDAKLRV
                     QMRAEVSRLQSELGVTTIYVTHDQTEAMTMGDRVAVLKKGELQQVDAPQFLYDHPANL
                     FVAGFIGSPAMNMVEADLVREDGGLYATFGSTRLRVADEVVSERPGIRAYEGRRVIAG
                     IRPENMEDASIMPVIPMDRRMKVDIVLREALGAEVLVHFTVDAPPVLTDDTRELVGEQ
                     SGPMAQASVIVQDLERALETGTSTFVARLDPRTHAAERQPLELAVDTARLHFFDPQTG
                     LGIYEDET"
     gene            1208..2575
                     /locus_tag="VIH70_04720"
     CDS             1208..2575
                     /locus_tag="VIH70_04720"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY8115697.1"
                     /translation="MSPTPPRFPEGFLWGAATSAFQVEGAVDEDGRGPSIWDTFCRTP
                     SKVRGGDTGDVACDQYHRYREDVALMEELGMGAYRFSIAWPRVQPDGRGPGNPRGLDH
                     YRRLVEQLNGRGIAAVVTLYHWDLPQALEDAGGWPNRDTAERFAEYAAIVHRALADGV
                     AHWITVNEPWVAAWLGYGTGVHAPGRTDDGLALAAGHHLLLAHALARDAIGEPVGIAL
                     NLEPHRPASETPPDGEIAHLADLHMNALFLDPLFGRGYPSELVERYRALFDRGFVREG
                     DLARIAGPLSFLGVNYYRPHTITTDVDGPDRTTEIPGSLGGWDVVPRGTPITSMGWPI
                     EPSGLLELLLRLDREYSPARIFLTENGAAFDDAPGPDGVVHDQARIAFLRDHVAAAGR
                     ALAAGVPLIGYLVWSLLDNFEWAEGYAKRFGLVHVDFETQSRTPKASARWFQALVETG
                     SSRPV"
     gene            2681..4012
                     /locus_tag="VIH70_04725"
     CDS             2681..4012
                     /locus_tag="VIH70_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8115698.1"
                     /translation="MKQLRLIVPFAALLLIAAACSSDEEGGGGETGTTGAALGENTGT
                     VNVLNAMEPNEAEAVQAAVDDNIQNADYTVEIESSADFEEQFPIRSEGGTLDIALVPQ
                     PGTVKQEAEAGNIVSLEDLGIDVSALEDSYGEYLFSLVEVDGEHYGIPTNVNLKSMVW
                     YPKDDFDKAGYQVPTTWDEMLALSDQIVADGGTPWCVGFESGSATGWPATDWMEDIML
                     RTAGVDTYDQWVSHEIPFTDPAVVTAAEDFGQIMFTDGYVLGGAADTPSISFGSAPLP
                     MFDEPPGCWLHRQASFIIGAAPFPEDAKAGVDYDWFPLPPIDQEGTLYAGEFAVVGTA
                     GARPEVVDFLQQFADVPIQCAQGADPGSSRISANINVGPDCYANQILADSAAVLTDAI
                     ANDTGRFDASDLMPGEVGAGSFWTGMVQYMKEGPDSLQGILEEIDSSWPSG"
     gene            4130..5281
                     /locus_tag="VIH70_04730"
     CDS             4130..5281
                     /locus_tag="VIH70_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015221934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8115699.1"
                     /translation="MSVTSEPAPATGLTGAPPSRRSTGLLVLVLRVAVAVAVPVATFY
                     ILWWTFEFLRDEHANRFIVVGVAIAVGVGGVFALYWGMNRAVDLFPARVRESVRPYVF
                     VGPCLVILAVYLVYPVINTVLISFKDANSESFVGLDNFRFVFTDSDMLRAIRNTAGWI
                     ILVPLAAVSIGLIYATLADRLRRGEGVAKSLIFLPLAISFAGASITWQLIYSYRPQGF
                     GSNIGLLNGVMIGLGQEPVKWLELQPWNNLLLMVIMVWMQTGFAMVVLSAAIKAIPDE
                     IIEAARIDGASEFQVFRRIMVPSILPTIVVVTTYMVINALKVFDIVFVQPASAETNGT
                     VVVAERMIRWFFQLNHNGRAAAIAVVLFVAVIPVMVWNIRRFREEEAIR"
     gene            5341..6228
                     /locus_tag="VIH70_04735"
     CDS             5341..6228
                     /locus_tag="VIH70_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8115700.1"
                     /translation="MEAAGRGGWFVRITIVVIVILWTIPTLGVLITSLRPEDAVNASG
                     WWTVFAHPFRATEWTIENYRIALDQGGFGNAFLNSLAVTIPSTVIPITIAAFAAYAFS
                     WMEFRGRYVMFVIVVGLLVVPLQMALIPILRLYAQGATLFGREIFPDLKLQGTFLGIW
                     LAHAGFGLPLAVYLLRNYIGSLPSSIIESARIDGADHFTIFWRLVVPLSVPAIAAFAI
                     FQFLWVWNDLLVAYVFLGGAGQNVVLTVALRELVGSRGENWHLLTSAAFISMALPLLV
                     FFALQKYFVRGLTAGAVKG"
     gene            6236..>6582
                     /locus_tag="VIH70_04740"
     CDS             6236..>6582
                     /locus_tag="VIH70_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115701.1"
                     /translation="MARSRRPAGAGAQPLSPRQVWRAILLSTLLLVPAYWGLLVGVVS
                     IASDDPERAPSAGPWTAFGLCLIPFVFLALAFLSQHPRAPRATVWAMLLCIVVGIPVS
                     AIAADAVTGFVAGV"
BASE COUNT          996 a         2162 c         2304 g         1120 t
ORIGIN      
        1 tctccaaggt gtactcggac ggcaccaccg ccgtctcgga cctctcgctc gacatcaagg
       61 acgaggagtt catcgtgctg gtcgggccgt ccgggtgcgg caagacgacc gcgctgcgga
      121 tggtggccgg gcttgagagc atcacccacg ggacgatcgc gatcggcgat cgggtcgtca
      181 acacggtgcc tccgaaggag cgggacatcg cgatggtgtt ccagaactac gcgctgtatc
      241 cgcacatgag cgtctacgac aacatggcgt tcgggctcaa gctgcgcaag ctgtccaagg
      301 aagagatcga tcgccgggtc atgcaggccg ccgagatcct cgggctcgag gagttcctcc
      361 agcgcaagcc gaaggcgctc tcgggtgggc aacgtcagcg cgtcgcgatg ggtcgcgcga
      421 tcgtgcgcga gccgcaggcg ttcctgatgg acgaaccgct gtccaacctc gacgcgaagc
      481 tccgcgtgca gatgcgtgcc gaggtctcac gcctgcagag cgagctcggg gtcaccacga
      541 tctacgtgac ccacgatcag accgaggcga tgacgatggg tgatcgcgtg gcggtcctca
      601 agaaaggcga gctgcagcag gtcgacgccc cacagttcct ctacgaccat ccggccaacc
      661 tgttcgtggc ggggttcatc gggtcgccgg cgatgaacat ggtcgaggcc gacctcgtgc
      721 gcgaggacgg tgggctctac gcaacgttcg gcagcacgag gctccgcgtg gccgatgagg
      781 tggtctcgga acgccccggg atccgcgcct acgaggggcg ccgagtgatc gcgggcatcc
      841 gcccggagaa catggaggac gcctccatca tgccggtcat cccgatggac cggcgaatga
      901 aggtcgacat cgtcctgcgt gaggcgctcg gggccgaggt gctggtgcac ttcacggtgg
      961 atgctccccc ggtcctgacc gacgacacgc gtgagctggt gggcgagcag agcgggccga
     1021 tggcccaggc cagcgtgatc gttcaggatc ttgagagggc cctcgagacc ggaacgtcga
     1081 cgttcgtagc gcgactcgat ccgcggacgc acgccgccga gcgccagccg ctcgagctcg
     1141 cggtcgacac cgcccgcctg catttcttcg atccgcaaac ggggctcggc atctacgagg
     1201 acgagacctg agtcctacgc ccccccggtt ccccgagggg ttcctgtggg gggccgcgac
     1261 gtccgcgttc caggtcgagg gtgccgtgga cgaggacggg cgaggaccct cgatctggga
     1321 cacgttctgc aggacaccgt cgaaggtgcg tggcggggat acgggagacg tggcgtgcga
     1381 ccagtaccac cggtaccgcg aggatgtcgc gctcatggag gagctcggga tgggcgccta
     1441 tcggttctcc atcgcgtggc cgagggtgca gccggacggc cgaggacccg gcaacccgcg
     1501 tgggctcgac cattaccgca ggctcgtcga gcagctgaac ggtcgcggca tcgccgccgt
     1561 cgtcaccctg taccactggg accttcccca ggcgctcgag gacgcgggtg gttggccgaa
     1621 ccgcgacacc gccgagcggt tcgccgagta cgccgcgatc gtgcaccgag cgctcgcaga
     1681 cggcgtggcg cactggatca cggtgaacga gccgtgggtc gcggcctggc tggggtacgg
     1741 caccggggtc cacgctcccg gtcgcaccga tgacggcctc gcgctcgccg ccggccatca
     1801 cctgctgctc gcccacgccc tggcgcggga cgcgatcggc gaaccggtcg ggatcgcgct
     1861 gaacctggag ccccatcgtc ccgcgtccga gactccgcca gacggcgaga tcgcccacct
     1921 ggcggacctg cacatgaacg ccctgttcct ggatccgctg ttcgggcgcg gatacccctc
     1981 ggagctcgtg gaacgctacc gagccctctt cgaccgcggg ttcgtgcgcg agggcgacct
     2041 cgcccgcatc gccggcccac tgtcgttcct cggcgtcaac tactaccgac cgcacacgat
     2101 cacgacggat gtcgatgggc ccgatcgcac caccgagatc cccggatcgc tcggcggctg
     2161 ggacgtggtg ccgcgcggaa cgccgatcac gtcgatgggg tggccgatcg agccctcggg
     2221 cctgctggag ctgttgctgc gactcgaccg cgagtactcg ccggcacgga tcttcctgac
     2281 cgagaacggg gccgcgttcg acgacgcgcc cgggccggac ggcgttgtcc acgaccaggc
     2341 acggatcgcg ttcctgcgcg accacgtcgc cgccgcgggc cgggcgctcg cggccggcgt
     2401 gccgctgatc ggctacctcg tctggtcgct gctggacaac ttcgagtggg ccgagggcta
     2461 cgcgaagcgc ttcggcctgg tccacgtcga tttcgagacc cagtcgcgga ccccgaaggc
     2521 gagtgcccgc tggttccagg cgctcgtcga gaccgggtcc tctcggcccg tttgaccgag
     2581 ccgggcaacg gggaggggtc agcgtgagag cggagccagg ccgagttgac ctccgatgac
     2641 gtacatgggg gagaatgggt cgggagaaag ggggcaggcg atgaagcaac tcaggctcat
     2701 cgtgccgttc gctgcgctgt tgctgatcgc ggcggcatgt tcgagcgacg aagagggagg
     2761 cggaggagag accggcacca ccggtgccgc tctcggggag aacaccggga cggtgaacgt
     2821 cctgaacgcg atggagccga acgaggcgga ggctgtccag gcggcggtcg acgacaacat
     2881 ccagaacgcc gactacaccg tcgagatcga gtcctcggcc gacttcgagg agcagttccc
     2941 gatccgttcc gagggcggga ccctcgacat cgcgctcgtg ccgcaacccg ggaccgtcaa
     3001 gcaggaggcg gaggccggca acatcgtgtc cctcgaggac ctgggcatcg acgtgagcgc
     3061 gctcgaggat tcctacggcg agtacctgtt ctcgctcgtc gaggtggacg gagagcacta
     3121 cggcatcccg acgaacgtga acctgaagag catggtgtgg tacccgaagg acgacttcga
     3181 caaggccggg taccaggtgc ccacgacgtg ggacgagatg ctcgcgctga gcgaccagat
     3241 cgtggccgac ggcggtacgc catggtgcgt gggcttcgag tctggcagtg cgaccggatg
     3301 gcccgccacc gactggatgg aagacatcat gctgcggacc gccggcgtgg acacgtacga
     3361 ccagtgggtc tcgcacgaga tcccgttcac cgacccggcg gtcgtcacgg cggccgagga
     3421 tttcggtcag atcatgttca ccgacgggta cgtgctcggt ggcgcggccg acacgccctc
     3481 gatctcgttc ggctcggcgc cgctgccgat gttcgatgag cctcctggct gttggctcca
     3541 ccgccaggcg agcttcatca tcggggcggc gccgttcccc gaggacgcga aggcgggcgt
     3601 ggactacgac tggttcccgc tcccgccgat cgaccaggag ggcacgctgt acgcaggtga
     3661 gttcgcggtc gtcgggaccg ccggtgcccg tccggaggtc gtcgacttcc tgcagcagtt
     3721 cgcggacgtg ccgatccagt gcgcgcaggg cgccgacccc gggtcgtcga ggatctcggc
     3781 caacatcaac gtcggccccg actgctatgc gaaccagatc ctggccgact cggcagccgt
     3841 cctgacggac gcgatcgcga acgacaccgg ccgattcgac gcctcggacc tgatgccggg
     3901 cgaggtcggt gccggcagct tctggacagg gatggtgcag tacatgaaag agggcccgga
     3961 ctcgctccag gggatcctgg aggagatcga ttcgagctgg ccctcggggt aggcggcccg
     4021 gggaggtagg aacgagccgg tcgaggctcg ccgtgccgga gggagcggcc gcagggcggc
     4081 cgctccctcc ggtttgcggc ggcggcttcg accgggggag gatgcgaacg tgagcgtcac
     4141 gtcggaaccg gcgcccgcta ccggcctgac cggagctcct ccatcgcgca ggtccaccgg
     4201 cctcctcgtg ctcgtgcttc gggtggcggt cgcggtcgcc gtccccgtcg cgacgttcta
     4261 catcctgtgg tggacgttcg agttccttcg ggacgaacac gccaaccggt tcatcgttgt
     4321 tggggtggcc atcgccgtgg gggtgggggg cgtcttcgcg ctgtactggg ggatgaaccg
     4381 agcggtggat ctgttccccg cccgtgtgcg cgagagcgtt cggccgtacg tcttcgtcgg
     4441 cccgtgcctg gtgatcctcg cggtctacct ggtctacccc gtgatcaaca ccgtgctgat
     4501 ctccttcaag gacgcgaaca gcgagagctt cgtcggcctg gacaacttca ggttcgtctt
     4561 cacggactcc gacatgctgc gcgcgatccg gaacacggcc gggtggatca tcctcgtgcc
     4621 gctggccgcc gtcagcatcg gcctgatcta cgcgacgctc gcggaccggc tccgtcgcgg
     4681 cgagggcgtc gccaagtcgc tgatcttcct gccgttggcg atctcgttcg ccggcgcctc
     4741 gatcacgtgg cagctgatct acagctaccg tccgcagggc ttcggatcga acatcggcct
     4801 gctgaacggg gtcatgatcg gtctcggcca ggaacccgtg aagtggctcg agctgcagcc
     4861 ctggaacaac ctgctcctga tggtgatcat ggtctggatg cagaccggct tcgcaatggt
     4921 ggtcctttcc gccgcgatca aggcgatccc tgacgagatc atcgaggccg cgcggatcga
     4981 cggagcctcg gagttccagg tcttccgccg gatcatggtg ccgagcatcc tgccgacgat
     5041 cgtcgtggtc acgacctaca tggtgatcaa cgcgctgaag gtcttcgata tcgtgttcgt
     5101 ccagcccgcc agcgccgaga cgaacgggac ggtggtggtc gcggaacgga tgatccgctg
     5161 gttcttccag ctcaaccaca acggccgcgc ggcggcgatc gcggtggtgc tgttcgtcgc
     5221 ggtgatcccg gtgatggtgt ggaacatccg ccgcttccgg gaagaggagg cgatccggtg
     5281 agctcgacca cggtcgaggc gcccccgaag gcacaggccc aaccgccgga aggggatcag
     5341 ctggaggcgg ccggccgcgg aggctggttc gtccgcatca cgatcgtcgt gatcgtgatc
     5401 ctgtggacga tcccgaccct cggcgtgctg atcacatccc tccggccgga ggatgccgtg
     5461 aacgcctccg gctggtggac ggtgttcgcc catccgttcc gggccacgga atggacgatc
     5521 gagaactacc ggatcgccct ggaccagggc ggcttcggga acgcgttcct gaacagcctg
     5581 gcggtgacga tcccctccac cgtgatcccg atcacgatcg cggcgttcgc cgcgtacgcg
     5641 ttctcgtgga tggagttccg cggccggtac gtgatgttcg tgatcgtcgt cgggctgctc
     5701 gtggtgccgc tgcagatggc gctgatcccg atcctgcgtc tgtacgcaca gggagcgacg
     5761 ctgttcggtc gcgagatatt ccccgatcta aaattgcagg ggaccttcct cgggatctgg
     5821 ctggcgcatg cgggcttcgg cttgcccctg gccgtgtacc tgttacggaa ctacatcggc
     5881 tcgctcccgt cctcgatcat cgagtccgca aggatcgacg gggccgacca cttcacgatc
     5941 ttctggcgcc tggtggttcc gctgtcggtc cctgcgatcg ccgcgttcgc catcttccag
     6001 ttcctgtggg tgtggaacga cctgctggtg gcctacgtgt tcctcggcgg cgccggccag
     6061 aacgtcgtcc tcacggtcgc gttgcgagag ctggtgggct cccgtggcga gaactggcac
     6121 ctgctgacgt ccgcggcgtt catctcgatg gcgctgccgt tgttggtgtt cttcgcgctc
     6181 cagaagtact tcgtacgtgg tctcacggcc ggtgcggtca agggctgatc tccggatggc
     6241 ccggtcgcgc cgaccggccg gcgccggggc ccagccgctg tcgccccggc aggtgtggcg
     6301 ggcgatcctg ctctcgacgc tcctgctggt gccggcgtat tgggggctgc tcgtcggggt
     6361 cgtctcgatc gcctcggacg accccgagcg cgccccgagc gccggtccct ggaccgcgtt
     6421 cgggttgtgc ctcatcccgt tcgtcttcct cgcgctcgcg ttcctgtccc agcacccgcg
     6481 tgcccctcgg gcgacggtct gggcgatgct cctgtgcatc gtcgtgggga tcccggtgtc
     6541 cgcgatcgcg gccgatgccg tcaccgggtt cgtcgccggc gt
//
LOCUS       DATBRK010000155         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2335590, whole genome shotgun sequence.
ACCESSION   DATBRK010000155 DATBRK010000000
VERSION     DATBRK010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335590"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VIH70_04745"
     CDS             complement(<1..1035)
                     /locus_tag="VIH70_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8115702.1"
                     /translation="MTEVTRDLGLEEAKEQITARGRERGFVTSDDLLESVPVDDFTPE
                     QVEEFLTQVEEHLRGEGIEVIEVPGDEVDGSAQRQLDVDLLKAPTNDPVRMYLKEIGK
                     VPLLTAAQEVDLARRIEAGEFATVLRELTEEEDRVDQKRLRLVTESVVAIRDHQVEKF
                     GKVEGIGRDRIGKTYKPKTREALDDFLRRVERDGQLAKRKLIEANLRLVVSIAKRYVG
                     RGMLFLDLIQEGNLGLIRAVEKFDYSKGYKFSTYATWWIRQAITRAIADQARTIRIPV
                     HMVETINKLVRIQRQLLQDLGREPLPEEIGRQMGIPADKVREILKVSQEPVSLETPIG
                     EEEDSHLGDFI"
     gene            complement(1112..>2287)
                     /locus_tag="VIH70_04750"
     CDS             complement(1112..>2287)
                     /locus_tag="VIH70_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY8115703.1"
                     /translation="GKAVGIGARILPGGSGRDEGPKYLNSPETPVYRKAEVLYNLQRA
                     KASATKLGELYLAEGYTDVIAMVRAGVTNTVATCGTALGEGHFRLASRFAQRMVLAFD
                     SDDAGARAAERAFEFVERFPVQPVVLILPEGLDPADFVDKHGGEALRELAAAAVPLVE
                     YMVRRTVGRHDLSTIEGQSAAVAAAVPLLEQLGDPVRQSQYAHLLAELAGVSEGSVLL
                     AIDQRLSGRPVEVQQAVKRASAQEKVEREMLRILAREAEAFGRIAPRLTDEHFRTTRH
                     RELLRLLRETDGRVSGSDAEGGDEQDVQALTALALEPLDGDLLPGYAEEVWVRLQEFL
                     LRRRSAALRRELQKMNPVTDPRYDEMFQRLIAADGELRRLRERGHDAALTKDLAPRS"
BASE COUNT          318 a          814 c          765 g          390 t
ORIGIN      
        1 gatgaagtcg ccgaggtggg agtcctcctc ctccccgatc ggggtctcga gggagacggg
       61 ctcctggctc accttcaaga tctcgcgcac cttgtcggcg gggatgccca tctgccgccc
      121 gatctcctcc gggagcggct cccggccgag atcctgcagg agctgtcgct ggatgcggac
      181 gagcttgttg atcgtctcga ccatgtgcac cgggatgcgg atcgtgcggg cctggtcggc
      241 gatcgcgcgg gtgatcgcct ggcggatcca ccacgtcgcg tacgtcgaga acttatatcc
      301 cttggagtag tcgaacttct cgaccgcgcg gatcaacccg aggttgccct cctggatcag
      361 gtcgaggaac agcatcccgc ggccgacgta ccgcttcgcg atcgagacca cgaggcgcag
      421 gttcgcctcg atcaacttgc gcttcgcgag ctgtccgtcg cgttcgacgc gccgcaggaa
      481 gtcgtcgagc gcctcgcggg tcttcggctt gtaggtcttg ccgatccggt cccgcccgat
      541 cccctccacc ttgccgaact tctcgacctg gtggtcgcgg atcgccacga ccgactcggt
      601 caccaggcga agccgcttct ggtcgacacg atcctcctcc tcggtcagct cgcggagcac
      661 ggtcgcgaat tcccccgcct cgatccgccg ggcgaggtcg acctcctggg cggctgtgag
      721 cagcgggacc ttgccgatct ccttcaggta catccgaacg gggtcgttcg tgggcgcctt
      781 caggagatct acgtcgagct ggcgctgggc ggacccgtcg acctcgtcgc cggggacctc
      841 gatgacctcg atcccctcgc cacgaaggtg ctcctcgacc tgggtgagga actcctcgac
      901 ctgttcgggc gtgaagtcgt cgacgggaac cgattcgagc aggtcgtcgg aggtcacgaa
      961 gccgcgctca cgcccgcggg cggtgatctg ttccttcgct tcctcgagac ccaggtcgcg
     1021 ggtgacttcg gtcaatgctc tcgctcccag aggctccagg tgggggaatg ttgggaagtg
     1081 tacgagacgt gaacgcccct gacccgcgtg gtcaggagcg cggcgcaagg tccttcgtca
     1141 aggcggcgtc gtggccgcgt tcccgcaagc gacgcagttc gccgtcggcc gcgatcagcc
     1201 gctggaacat ctcgtcgtat ctcgggtcgg tcaccgggtt catcttctgc agctcacgac
     1261 ggagcgctgc gctgcggcgt cgcaggagga attcctggag cctcacccag acctcttccg
     1321 catagccggg gagcagatcg ccgtcgagcg gctcgagcgc cagcgccgtg agcgcctgga
     1381 cgtcctgctc gtcaccgccc tcggcgtccg aaccgctcac gcggccgtcg gtctcccgga
     1441 gcaggcgcaa cagctcccgg tgccgggtcg tccggaagtg ctcgtcggtc aggcgcggcg
     1501 cgatccgacc gaacgcctcg gcctcgcggg cgaggatcct gagcatctcc cgctcgacct
     1561 tctcctgcgc ggacgctcgc ttgacggcct gctgaacctc gaccgggcgt cccgagagcc
     1621 gctggtcgat cgccaggagg accgatccct ccgacacacc ggcgagctcc gcgagcaggt
     1681 gcgcgtactg gctctgccgg accgggtcgc ccagctgttc gagcagcggg acggccgcgg
     1741 cgaccgccgc gctctggcct tcgatcgtcg agaggtcgtg gcggcctacg gtccttcgca
     1801 ccatgtactc gacgagcggg acggccgccg ccgcgagctc gcggagggcc tcgcccccgt
     1861 gcttgtcgac gaagtccgcc gggtccaggc cctcgggcag gatcaggacg accggctgga
     1921 ccgggaaccg ctcgacgaac tcgaaggcgc gctcggcggc gcgcgcgccc gcgtcatccg
     1981 agtcgaaggc gagcaccatc cgctgcgcga accgcgacgc gaggcggaag tgcccctctc
     2041 cgagcgcggt cccgcacgtc gcgaccgtgt tcgtcacgcc ggcccgaacc atcgcgatca
     2101 cgtcggtgta gccctcggcg aggtacagct cgccgagctt ggtggccgac gccttggcgc
     2161 gctgcaggtt gtagagcacc tcggcctttc ggtacaccgg cgtctcgggc gagttcaggt
     2221 acttcggccc ctcgtcgcgc ccgctgccgc cgggcaggat cctcgcgccg atcccgaccg
     2281 ccttgcc
//
LOCUS       DATBRK010000156         7385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2480649, whole genome shotgun sequence.
ACCESSION   DATBRK010000156 DATBRK010000000
VERSION     DATBRK010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2480649"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /gene="miaA"
                     /locus_tag="VIH70_04755"
     CDS             complement(<1..923)
                     /gene="miaA"
                     /locus_tag="VIH70_04755"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020216803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY8115704.1"
                     /translation="MVARDGSGAEPPLLALLGPTAAGKTEAGIAVAERVGAEIVSVDS
                     MLVYLGMDIGTAKPTAEQRERVPHHLLDVAEPSEPFSVARYQDLAWAAIADIRSRGRR
                     VLLVGGSALYERAIVDELGFPGTDPDTRGGLEAEAAVVGATGLHERLAASDPAAAAKI
                     EPGNVRRTVRALEVAELTGKPFSSFAGGWETYEPDRVRAAGVSVSAEVLRTRIETRVA
                     EMLERGWLDEVRGLVERGFGAWLTSAQAIGYAELARHLAGELTLDAAIAGTVKRTRAL
                     ARRQLAFFRRDPRIRWFEAGQAGAGPLVEDVV"
     gene            complement(923..2326)
                     /gene="miaB"
                     /locus_tag="VIH70_04760"
     CDS             complement(923..2326)
                     /gene="miaB"
                     /locus_tag="VIH70_04760"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005090296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY8115705.1"
                     /translation="MAQPRYLLRTFGCQMNEHDSERIAGLLTADGYVATEEAADANVV
                     VFNTCAIRENADNRLYGNLGHLRPLKQANPRMRIVVAGCLAQKDGGTIRARAPWVDVV
                     VGTHALPGLIELLHRAETEGPQLDIREQTEIFPSALPALREVAHHAWVSIAVGCDNAC
                     TFCIVPLVRGPQLSRPIGDVLAEVQGLARRGVVEITLLGQNVNTYGRDLTVPGSARRP
                     LFADLLRAVSRVEGLRRIRFTSPHPHDFTPDVIDAMAESDKVCEHIHFPLQSGSDRVL
                     RAMQRSYRRERYIGWLERIRAAIPGIAVSTDIIVGFPGETEEDFEDTLDVVRRARFDQ
                     AFTFQYSPRPGTRAAEMEGQLDKPEVQERFDRLVALQEAIALERMREQVGSWAEVLIE
                     GLGRKGRARGRTRGNRVVHVGGEHAPGTFLDVRIVGAHPHHLDGELAAGGQGGSRSER
                     ERASGSPPERREPAAVG"
     gene            complement(2354..3886)
                     /gene="rny"
                     /locus_tag="VIH70_04765"
     CDS             complement(2354..3886)
                     /gene="rny"
                     /locus_tag="VIH70_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY8115706.1"
                     /translation="MVPVVVGIVALALGVAIGFVIRRAVARTQAESAEGRAQKVILEA
                     EREAERLTREALVEAKDEIGALRRETEEDVRTRRDEIKRQEERLTDKERIVDRKVDDA
                     DRRSEELDDRDRKLALVREQLEHSAVQHRAALERIARLTASEAKEALRAQVVDEAKRE
                     AMATVREIEQRAREEGEERARKIVTIAIQRVASDQTSESTVSVFSLPSEDMKGRIIGR
                     EGRNIRAFEATTGVNLIIDDTPEAVVLSSFDPVRRETARLTLEKLVQDGRIHPARIEE
                     TFERSQKEIDEQIKRAGEEAVLDTGITDMHPEMIRLLGRLQFRTSYGQNVLKHLVESA
                     HIAAAIASELGIDPTVPKRGALLHDIGMAVTHEVEGSHAIIGAEIARRMKESPEVVHC
                     IESHHGEVEQRTIDAVIAQIADGISGGRPGARRESLETYIKRLERLEEICTSYPGVEK
                     VYAMQAGREVRVMVKPEDIDDLSAQVMARDIAKQVEEELQYPGQIKITVVRETRSVEY
                     AK"
     gene            complement(4159..4650)
                     /locus_tag="VIH70_04770"
     CDS             complement(4159..4650)
                     /locus_tag="VIH70_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY8115707.1"
                     /translation="MATKRTVKQPLDCHERALRLLAVRPRARRELEARLRQAGFEAAE
                     VSSELDRLEEVGLLDDGAFARELAEHHLTVRGSGRRAVAGALASKGVSRETIEQTLAG
                     LDEDESVRALEVATDRARRLTSLRREVAYGRLVSFLARRGYDGGISREAARAVLGLDD
                     RHA"
     gene            complement(4682..5797)
                     /gene="recA"
                     /locus_tag="VIH70_04775"
     CDS             complement(4682..5797)
                     /gene="recA"
                     /locus_tag="VIH70_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY8115708.1"
                     /translation="MDRDKMLDVTLTQIEKQFGKGAVMKLGEHPMGAGISVIPTGSLA
                     LDIALGVGGIPRGRVVEVFGPEGSGKTTVCLHVIAEAQIHGGIAAFIDAEHALDPTYA
                     QRLGVNIDELLVSQPDSGEQALEIADMLVRSGALDVVVIDSVAALVPRAEIEGEMGDT
                     HVGLQARLMSQAMRKLSGTLSRFDTTAIFINQLREKIGVMFGNPETTPGGRALKFYSS
                     VRLDVRKIENLKDGTDVVGSRTRVKVVKNKVAPPFRQCEFDIMYGVGISKEGSLLDVG
                     VDLEIVKKSGAWFTYEGDQLGQGRENARQFLAEHTDIATELERRVREAVGLTAFGAEG
                     DDTPIEVVPDLAETDEQIAPKAAKADKERETTGARAG"
     gene            complement(6030..6608)
                     /gene="thpR"
                     /locus_tag="VIH70_04780"
     CDS             complement(6030..6608)
                     /gene="thpR"
                     /locus_tag="VIH70_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY8115709.1"
                     /translation="MARDRASRPEARPLRLFVATEIPDEIRGALAEAVTPVRERFPKA
                     RWVPIQNQHVTVKFLGSTYPRLVEWVTATVRQVATRNAAFTTRVEGLGAFPNARRARV
                     LWAGLDDAASAFGRLAADLDEGLSEQFKPEKRAFTPHLTVARFEPTVSLDPHEIAFES
                     EPFEVRRIVLFRSHLRRPAPVYEPMEAFRLSG"
     gene            complement(6608..>7385)
                     /locus_tag="VIH70_04785"
     CDS             complement(6608..>7385)
                     /locus_tag="VIH70_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEY8115710.1"
                     /translation="REMMEGTILPELATLAGPASLVSRTLRVYGLAESRISELLDDLF
                     HASSNPTVAYLAGGGEVHVRLTAKGSPAEADALLDPLEERVRERLGDHVYGSGDNAME
                     VVVGRLLAERGRTLACAESLTGGGLGERITAVPGASAYFLGSAVTYSIEAKRSILHVS
                     AETLAGPGPVSRECAAEMASGARRLFGADAAVALTGAAGPEAHGGAEPGRVWIGLDAD
                     GVEHQHGFRWPFERELIRRFAEMAALDLVRRHLLGLSFGD"
BASE COUNT          985 a         2788 c         2470 g         1142 t
ORIGIN      
        1 acgacgtcct cgacgagcgg cccggccccg gcctgccccg cctcgaacca gcggatcctc
       61 gggtcccgcc ggaagaaggc gagctgccgg cgcgcgagcg ccctcgtgcg cttcaccgtg
      121 cccgcgatcg ccgcgtcgag cgtgagctcg ccggcgaggt gacgggccaa ctcagcgtac
      181 ccgatggcct gggccgaggt gagccaggcg ccgaaacctc gttcgacgag cccgcggacc
      241 tcgtcgagcc acccgcgctc gagcatctcg gcgacgcgcg tctcgatccg cgttcggaga
      301 acctcggccg acaccgagac acccgccgcc cgaacccgat cgggttcgta cgtctcccac
      361 cccccggcga agctggagaa cggcttacct gtcagctcgg cgacctcgag cgcccgcacg
      421 gtgcgccgaa cgttccccgg ctcgatcttc gcggcggcgg ccgggtcgga cgccgcgagg
      481 cgctcgtgga gtccggtcgc cccgaccacc gcggcctcgg cctccagtcc gccgcgcgtg
      541 tccgggtcgg tccccgggaa cccgagctcg tcgacgatcg cgcgctcgta cagggccgag
      601 ccgccgacga gcagcacccg gcgcccgcgc gaacggatgt cggcgatcgc cgcccaggcc
      661 aggtcctggt accgcgcgac gctgaacggc tcggagggct cggccacgtc gaggaggtgg
      721 tgcgggaccc gttcccgctg ctccgcggtc ggcttggcag tcccgatgtc catccccagg
      781 tacacgagca tcgagtcgac cgagacgatc tcggcgccga cgcgttcggc gaccgcgatc
      841 cccgcctcgg tcttgcccgc ggcggtcgga ccgaggagcg ctaggagcgg tggttcggcc
      901 ccggagccgt cgcgtgcgac catcacccga ccgccgccgg ctcccgccgt tccggggggc
      961 taccgctcgc gcgttcgcgc tcgctccttg agcccccctg gcctcccgcc gcgagctcgc
     1021 cgtccaggtg gtgcgggtgg gcgcccacga tccgcacgtc gaggaacgtc cccggcgcgt
     1081 gctcgccgcc gacgtgcacg acgcggttgc cccgcgtccg gccccgcgcg cggcccttgc
     1141 gaccgagccc ctcgatcagc acctcggccc acgagcccac ctgctcgcgc atccgctcga
     1201 gcgcgatcgc ctcctggagc gcgaccagcc ggtcgaaccg ctcctggacc tccggcttgt
     1261 cgagttgacc ctccatctcg gcggcccgcg tccccggccg aggcgagtac tggaacgtga
     1321 acgcctggtc gaaacgcgcg cgccgaacca cgtcgagcgt gtcctcgaag tcctcctcgg
     1381 tctcgccggg gaacccgacg atgatgtcgg tgctcaccgc gatgccgggg atcgcggcgc
     1441 gaatccgttc gagccagccg atgtaccgct cgcgccggta cgaacgctgc atcgccctca
     1501 gtacccgatc cgagcccgat tgcaacggga agtggatgtg ctcgcacacc ttgtcgctct
     1561 cggccatcgc atcgatcacg tcgggcgtga agtcgtgggg gtgcgggctc gtgaaccgga
     1621 tccggcggag cccctccacc cggctcacgg ctcggagcag gtcggcgaac agcggacgcc
     1681 gagccgaccc cggcacggtg aggtcccggc cgtaggtgtt cacgttctgg ccgagcagcg
     1741 tgatctcgac cacgccccgg cgggcgaggc cctgcacctc ggccagcacg tcgccgatcg
     1801 ggcgcgagag ctgcgggccg cgcacgagcg gcacgatgca gaacgtgcac gcgttgtcgc
     1861 accccaccgc gatcgagacc cacgcgtggt gcgcgacctc gcgcagcgcc ggcagggcgc
     1921 tcgggaagat ctcggtctgt tcgcggatgt cgagctgcgg gccctcggtc tcggctcgat
     1981 ggagaagctc gatcagcccg ggcagggcgt gggtgccgac gacgacgtcc acccaggggg
     2041 cgcgtgcgcg gatcgtgccg ccgtccttct gcgccaggca cccggcgacg acgatccgca
     2101 tccgggggtt cgcctgcttg agcggccgca ggtgcccgag gttgccgtag agccggttgt
     2161 ccgcgttctc gcggatcgcg cacgtgttga acacgacgac gttcgcgtcg gcggcctcct
     2221 cggtcgccac gtagccgtcg gcggtgagca ggccggcgat ccgctcggag tcgtgctcgt
     2281 tcatctggca cccgaaggtg cggagcaggt acctcggctg ggccatcgtc ctcaggctac
     2341 cctcgcgcgc gcctcacttg gcgtactcga ccgaacgcgt ctcgcgcacg accgtgatct
     2401 tgatctgccc ggggtactgc aattcctcct cgacctgctt ggcgatgtcc cgcgccatca
     2461 cctgcgccga gaggtcgtcg atgtcctcgg gcttgaccat cacgcggacc tcgcggccag
     2521 cctgcatcgc gtagaccttc tccacgccgg ggtagctcgt gcagatctcc tcgagtcgct
     2581 cgaggcgttt gatgtaggtc tcgaggctct cgcggcgggc tccggggcgg ccgcccgaga
     2641 tcccgtcggc gatctgcgcg atcaccgcgt cgatcgtccg ctgctcgacc tccccgtggt
     2701 gggactcgat gcagtggacc acctcgggcg actccttcat tcgccgggcg atctcggcgc
     2761 cgatgatcgc gtgcgagccc tcgacctcgt gcgtcaccgc catgccgatg tcgtgcagca
     2821 gcgccccgcg cttggggacc gtcgggtcga tcccgagctc ggacgcgatc gccgcggcga
     2881 tgtgggccga ctcgacgaga tgcttgagga cgttctggcc gtacgaggtc cggaactgca
     2941 gccgcccgag gagccggatc atctcggggt gcatgtcggt gatcccggtg tcgaggaccg
     3001 cctcctcacc cgcgcgcttg atctgctcgt cgatctcctt ctgcgatcgc tcgaacgtct
     3061 cctcgatccg cgccgggtgg atccgcccgt cctgcacgag tttctcgagc gtgagccgcg
     3121 ctgtctcgcg ccgcactggg tcgaagctcg acagcaccac cgcctcgggc gtgtcgtcga
     3181 tgatcaggtt caccccggtc gtcgcctcga aggcgcggat gttccggccc tcacgaccga
     3241 tgatgcgtcc cttcatgtcc tcgctcggga gcgagaacac cgagaccgtc gactcgctcg
     3301 tctggtcgga ggcgacgcgc tggatcgcga tcgtcacgat cttgcgggcg cgttcctcac
     3361 cctcctcgcg cgcgcgttgc tcgatctccc gaaccgtggc catcgcctcg cgcttggcct
     3421 cgtcgaccac ctgcgcgcgg agcgcctcct tcgcctcgga cgccgtgagc cgggcgatcc
     3481 tctcgagcgc ggcgcgatgc tggaccgccg agtgctcgag ctgctcgcgg accagcgcga
     3541 gctttcggtc tcgatcgtcc agctcctccg aacgccggtc cgcatcgtcg accttgcggt
     3601 cgacgatccg ctccttgtcc gtaaggcgtt cctcctggcg cttgatctcg tcccgccggg
     3661 tgcgaacgtc ctcctcggtc tcgcgccgga gcgcgccgat ctcgtccttc gcctcgacca
     3721 gggcctcgcg cgtgaggcgc tccgcctcgc gctcggcctc caggatcacc ttctgcgctc
     3781 tgccctcggc gctctcggcc tgcgtcctgg cgaccgctcg tcggatcacg aacccgatcg
     3841 ccacgcccag ggctagggca acgatcccca ccaccaccgg taccatcggt tcaccgcctc
     3901 gtccgtagac acggaacagc cgaaccgagg cccatcgggc actgttcggc cgtgcgtccg
     3961 aaggatgcgg actagagcga tgccggggcc ggcggccccg ctcgccctcc gatccggtgt
     4021 gctccctgtg aggtctggtt tctcaaggtt tcaggcacgg ttgaacgttg accaacagat
     4081 gaacctcgta cgaacagagc ctacggcgtg cgctcggcag aggcaagggt ggccgcctcg
     4141 cggcaccgcc ggcggcgctc aggcgtggcg gtcgtcgagc cccaggaccg ccctcgcggc
     4201 ctcccgagag atgccgccgt cgtagccgcg ccgggccagg aacgagacca ggcgcccgta
     4261 cgccacctcg cgcctcaggc tcgtgaggcg ccgagcccga tccgtcgcga cctcgagcgc
     4321 gcggacgctc tcgtcctcgt cgaggccggc cagggtctgc tcgatcgtct cgcgtgacac
     4381 gcccttcgac gcgagcgcgc ccgcgacggc ccgccgcccg ctcccgcgga ccgtcaggtg
     4441 atgttcggcg agctcgcgcg cgaaggcccc gtcgtcgagc agcccgacct cctcgagtcg
     4501 gtcgagctcc gacgacacct cggccgcctc gaaccccgcc tggcgcagcc gcgcctccag
     4561 ctcgcgccgc gcccgcggac ggaccgcgag cagccgaagc gcgcgctcgt ggcagtcgag
     4621 cggttgcttg acggtgcgct tggtcgccat gtgaggacgc cgggggcgag cgggccttcc
     4681 cctaacccgc tcgtgccccc gtcgtctcac gctccttgtc ggccttggcc gccttcggag
     4741 caatctgctc gtcggtttcc gcgaggtccg ggacgacctc gatcggcgtg tcgtcgccct
     4801 cggcgccgaa cgccgtcagg ccgaccgcct cgcgcacccg gcgttcgagc tcggtcgcga
     4861 tgtccgtgtg ctcggcgagg aactggcgcg cgttctcgcg accctgcccg agctgatcgc
     4921 cctcgtacgt gaaccacgct ccgctcttct tgacgatctc gaggtcgacg ccgacgtcga
     4981 ggaggctccc ttccttggag atgccgacgc cgtacatgat gtcgaactcg cactggcgga
     5041 acgggggcgc gaccttgttc ttcacgacct tgacgcgcgt ccgcgatccc accacgtcgg
     5101 tcccgtcctt caggttctcg atcttgcgca cgtcaagccg gaccgaggag tagaacttca
     5161 gcgcgcggcc gccgggggtc gtctcggggt tgccgaacat cacgccgatc ttctcgcgga
     5221 gctggttgat gaagatcgcc gtggtgtcga accgcgagag cgtgcccgag agcttgcgca
     5281 tcgcctgcga catcagccgc gcttgcaggc cgacgtgggt gtcgcccatc tccccctcga
     5341 tctcggcccg cgggacgagc gccgcgaccg agtcgatcac gaccacgtcg agcgcgcccg
     5401 agcgcacgag catgtcggcg atctcgagcg cctgctcgcc ggagtccggc tgcgagacca
     5461 ggagctcgtc gatgttgacg cccagacgct gggcatacgt cgggtcgagc gcgtgctcgg
     5521 cgtcgatgaa cgcggcgatg ccgccgtgga tctgcgcctc ggcgatcacg tggaggcaga
     5581 ccgtcgtctt gcccgagccc tcgggcccga agacctcgac cacccgcccg cgcgggatgc
     5641 cgcccacgcc cagcgcgatg tcgagcgcga gcgagcccgt cgggatcacc gagatgccgg
     5701 cgcccatcgg gtgctccccg agcttcatca ccgcgccttt gccgaactgc ttctcgatct
     5761 gcgtgagcgt gacgtccagc atcttgtccc ggtccatccg cgaagcctcc ttctgtccgc
     5821 ccctcgtgtt ctctcgggcc cagcgggcga ccgcctgcga gagctcctcc tggtcgggag
     5881 ggcgaacgct accgctcggg tctgacacgg attacacctc tgtagttacc ggactgcaac
     5941 ccgtggagcg tactcgaaca ggtgttcgag gtcaaggagc tcggggtgat ccccgacgac
     6001 gccggccgag ccctttcgag ggaggccgat cagcctgaaa ggcgaaaggc ctccatcggc
     6061 tcgtagaccg gggccgggcg ccggagatga ctgcggaaca gcacgatccg gcggacctcg
     6121 aacggctcgc tctcgaacgc gatctcgtgc gggtccaggg acacggtcgg ctcgaaccgg
     6181 gcaacggtca ggtgcggcgt gaacgctcgt ttctccggct tgaactgttc ggacagaccc
     6241 tcgtccaggt cggccgcgag tcgcccgaac gccgatgccg cgtcgtccag gcccgcccac
     6301 aggacgcggg cgcgccgtgc gttcgggaac gctcccaggc cctcgacccg ggtggtgaac
     6361 gccgcgttgc gcgtcgccac ctgcctcacc gtcgccgtca cccactccac gagccgcggg
     6421 taggtggagc cgaggaactt caccgtcacg tgctggttct ggatcggcac ccatcgggcc
     6481 ttcgggaacc gctcgcggac cggtgtcacc gcctcggcga gggcaccgcg gatctcgtcc
     6541 gggatctcgg tcgcgacgaa cagccgcagc ggcctcgcct ccggccggga cgcccggtcg
     6601 cgcgccatca gtccccgaac gacagcccga gcaggtggcg tcggaccagg tcgagcgccg
     6661 ccatctcggc gaagcgccgg atcagctccc gctcgaacgg ccaccggaag ccgtgctggt
     6721 gctcgacacc gtcggcgtcc aggccgatcc acacccgacc gggttcggcg ccgccgtgcg
     6781 cctcgggccc ggcggcgccg gtgagcgcga ccgcggcgtc ggccccgaac agccgccgcg
     6841 cgcccgacgc catctcggct gcgcactccc ggctcaccgg tcccggaccg gcgagcgtct
     6901 cggccgacac gtgcaggatc gaccgcttgg cctcgatcga atacgtcacg gccgagccga
     6961 ggaagtacgc ggaggcgccc ggcaccgccg tgatgcgctc gcccaggccg ccgcccgtga
     7021 gcgattcggc gcacgcgagc gtccgtcccc gttcggccag cagccgcccg accacgacct
     7081 ccatcgcgtt gtcccccgag ccgtagacgt ggtcgcccag ccgctcgcgc acccgctcct
     7141 cgagcgggtc gagcagggcg tccgcctctg cgggcgaacc cttggccgtc agccggacgt
     7201 gcacctctcc cccgccggcg aggtaggcga ccgtcgggtt cgaggacgcg tggaacaggt
     7261 cgtccaggag ctcggagatc ctcgactccg cgagcccgta gacgcggagc gtccgcgaga
     7321 cgagcgacgc gggcccggcg agcgtcgcga gctcgggcag gatcgtcccc tccatcatct
     7381 cccgc
//
LOCUS       DATBRK010000157         4159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_604383, whole genome shotgun sequence.
ACCESSION   DATBRK010000157 DATBRK010000000
VERSION     DATBRK010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604383"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VIH70_04790"
     CDS             <1..526
                     /locus_tag="VIH70_04790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY8115711.1"
                     /translation="VTRGILEILDERELRGVLAHELSHVANRDILIGSIAAAIGMSIT
                     FLARFALWFGSGNDRNGIGIVGLLLAWVLAPIAAAIIQMAVSRSREYQADESGAILSR
                     DPDALASALRKLENAAGKVPVPASVSPAEAHLFIVNPFRGRQASRSLANLFSTHPPTA
                     ERVARLEQIKQEIG"
     gene            complement(547..1215)
                     /locus_tag="VIH70_04795"
     CDS             complement(547..1215)
                     /locus_tag="VIH70_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8115712.1"
                     /translation="MSEVRAVLLDLYDTLAWTEWPTMRAELEDRFGLGEAELLRAFTA
                     TREARSTGAFDSAEGDLAAILDAAGVPSDPVLVRELNDARVKAFADNGVHLWDDSVPT
                     LRELRARGLRTVIVSNCDHATRPIVDDLGLEHEADAIVLSFEVGVAKPDPGIYRAALS
                     AVGARPQEAVFVDDQDWYCEGAEALGIRSFLIRRDDADPAEGFGAAADRDVLHDLQSL
                     LDLV"
     gene            complement(1212..2561)
                     /locus_tag="VIH70_04800"
     CDS             complement(1212..2561)
                     /locus_tag="VIH70_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY8115713.1"
                     /translation="MHEYTPVTDSLTEAVVDYTRRRIADPQPLDHPVLPEDLTRSAGS
                     TITPDGIGGREALRIWEEVLAPATITTDHPSFLAFVPGAPSKASVLFDLAVSASEVYG
                     GSWLEGGGAVWAENQVLGWLAELAGMPKDSGGCFVSGGTAGNLSALVAARHAASDARG
                     GERPSRWQIACADTVHSSVASAARVMDVDLLSAPHDERGRLTGPALAAALDRTEPGGP
                     FAVVASAGSTNAGTIDDLEGVAAVCRERGLWLHVDGAYGGAGLLAPSVRDRFRGIEDV
                     DSFIVDPHKWLFAPYDSCALIYREPARARSAHRQDASYLDALNATSEWNPSDYAHHLS
                     RRPRGLPLWFSLATYGTDAYRDAVERVLSITRETADEIRRRPELELVLEPELSVVLFR
                     RLGWRPREYEAWWRTALTEQVGFVQPTSWEGEKVGRLCFVNPLTTIEDVRAILDLAA"
     gene            complement(2612..3997)
                     /locus_tag="VIH70_04805"
     CDS             complement(2612..3997)
                     /locus_tag="VIH70_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8115714.1"
                     /translation="MTDVERLKSRAAEIAEREGRRLLDRTPGSESLHQRALRSLPNGV
                     ASNFQAGDPYPTYLRRGMGSKVWDVDGNEYLDFHGGFGVNVVGHAHPKIVEAIRAVAD
                     QGIHFAVTTETTVALAEAICERFQLEQMRFVNSGTEATMDAIRVARAATGRDTIVKME
                     GSYHGHHDSVLFSVVPEADVLDMRFNVGAEGRAWYTTEPTSRGIPKVLTETVIIVPFN
                     DASAVEQAFADNPGEIAAVILEPVMMNIGLVVPQPGYLQALRDICERNGAALIYDEVK
                     CGGTIAYGGTTDRYGVRPHLAAYAKAVGGGVTIGAFGGEARFMEWVTKGAAQQGTFNG
                     NPLSSAAGLAALTQVLTKDAYDHLGKLGTLLAEGCQRAIDEHDLPAHTMDLGAKGCVS
                     YRKEQLVNYRDFLETIPELFYASFTWMVNRGIFMTPGDEEQWTISVQHTEEDIQRYVD
                     AYAAFCAELVA"
BASE COUNT          610 a         1470 c         1445 g          634 t
ORIGIN      
        1 ggtcacccgc gggatcctcg agatcctgga cgaacgcgag ctgcggggcg tgctggcgca
       61 cgagctctcg cacgtcgcca accgggacat cctgatcggt tcgatcgcgg cggccatcgg
      121 gatgagcatc acgttcctcg cgcgattcgc cttgtggttc gggtcgggca acgatcgcaa
      181 cgggatcggc atcgtggggc tgctgctggc ctgggtcctc gccccgatcg cggccgcgat
      241 catccagatg gccgtgagcc gctcgcgcga gtaccaggcc gacgagtccg gtgcgatcct
      301 gtcccgcgac cccgacgcgc tggcgagcgc gctccgcaag ctcgagaacg ccgccggcaa
      361 ggtcccggtg ccagcgtcgg tgagccccgc cgaggctcac ctgttcatcg tgaacccttt
      421 ccgcggccgc caggcgtcaa ggagcctggc gaacctgttc tcgacccacc ccccgacggc
      481 cgagcgcgtg gcgaggctcg agcagatcaa gcaggagatc ggctagctcg tttcgggcgc
      541 ccccgatcag acgaggtcga gcagcgactg gaggtcgtgg agcacgtcgc ggtctgcggc
      601 ggcgccgaac ccctcggcgg ggtcggcgtc gtcgcgtcgg atcaggaacg acctgatgcc
      661 gagcgcctcc gcaccctcgc agtaccagtc ctggtcgtcg acgaagacgg cctcctgggg
      721 ccgcgcaccg acggccgaga gcgccgcccg gtagatcccg gggtccggct tggccacgcc
      781 cacctcgaac gagagcacga tcgcgtccgc ctcgtgctcc agcccgaggt cgtcgacgat
      841 cgggcgggtg gcgtggtcgc agttgctcac gatcacggtt cggagcccac gagcgcggag
      901 ctcgcgcagg gtcgggaccg agtcgtccca cagatggacg ccgttgtcgg cgaaggcctt
      961 gacgcgggca tcgttgagct cgcggaccag gaccggatct gacggaaccc cggccgcatc
     1021 caggatggcg gcgaggtcgc cctcggcgct gtcgaacgcg ccggtgctcc gagcctcgcg
     1081 cgtggcggtg aaggcgcgaa ggagctccgc ctcgccgagc ccgaatcgat cctcgagttc
     1141 ggcacgcatc gtcggccatt cggtccatgc aagcgtgtcg tacaggtcga gcagaacggc
     1201 ccggacctcg ctcacgcggc caggtcgagg atcgcccgca cgtcctcgat cgtcgtgagg
     1261 gggttgacga agcagagccg gcccaccttc tcgccctccc atgacgtggg ttggacgaag
     1321 cccacctgtt cggtgagcgc cgttcgccac caggcctcat actcccgcgg ccgccatccg
     1381 agccttcgga acagaaccac ggacagctcc ggttccagga cgagctcgag ctcgggccgc
     1441 cggcggatct cgtcggccgt ctcccgcgtg atcgacagga cccgctcgac cgcgtcccga
     1501 taggcgtcgg tcccgtaggt cgcgagcgag aaccacaggg ggagcccgcg cggacgccgg
     1561 gagaggtggt gcgcgtagtc gctcgggttc cattcggacg tggcgttcag ggcgtcgagg
     1621 tacgaggcgt cctgacgatg agccgagcgc gccctcgcgg gctcgcgata gatgagcgcg
     1681 caggagtcgt acggggcgaa cagccacttg tgcgggtcga cgatgaacga atcgacgtcc
     1741 tcgatgccgc ggaaccggtc tcggacgctc ggcgcgagga gccccgcgcc cccgtacgcg
     1801 ccgtcgacgt ggagccagag ccctcgttcg cggcagacgg ccgcgacccc ctcgaggtcg
     1861 tcgatcgttc cggcgttggt ggaaccagcg ctcgcgacca ccgcgaacgg accacccggt
     1921 tcggtgcgat cgagcgctgc cgcgagcgct gggccggtca ggcggccgcg ttcgtcgtgc
     1981 ggggcggaga gcaggtcgac gtccatcacc cgcgccgccg acgcgaccga cgaatgcacc
     2041 gtgtcggcgc aggcgatctg ccagcgcgat gggcgttccc cgccccgagc atcgctcgcg
     2101 gcgtggcgcg ccgcgaccag ggccgacagg ttccccgccg taccccccga cacgaagcag
     2161 ccaccggagt ccttcggcat cccggcgagc tcggcgagcc accccagcac ctggttctca
     2221 gcccacacgg cgccgcctcc ctcgagccag ctccccccgt agacctcgga cgcgctcacg
     2281 gccaggtcga acaggacgct cgccttgctc ggcgcgccgg gcacgaacgc gaggaagctc
     2341 gggtggtcgg tcgtgatggt tgccggggcg agcacctcct cccagatccg cagggcctcc
     2401 cggccaccga tcccgtccgg cgtgatcgtg gatccggccg accgcgtgag gtcttccgga
     2461 agcacgggat ggtcgagcgg ctgcggatcc gcgatgcgcc ggcgcgtgta gtcgacgacg
     2521 gcctccgtca gcgagtcggt caccggggtg tattcatgca tcgactgcat cggcgctccg
     2581 gtctcgcggc ccctgccagg tgtcggggcg gctaggccac cagctcggcg cagaacgccg
     2641 cgtacgcgtc cacgtagcgc tggatgtcct cctcggtgtg ctgcacggag atcgtccact
     2701 gctcctcgtc gccgggcgtc atgaagatgc ctcggttcac catccacgtg aacgacgcat
     2761 agaagagctc ggggatcgtc tcgaggaagt cgcggtagtt gaccagctgc tccttgcggt
     2821 acgagacgca gcccttggcg ccgaggtcca tcgtgtgcgc cgggaggtcg tgctcgtcga
     2881 tcgcacgctg gcagccctcc gcgagcaacg tcccgagctt gcccaggtgg tcgtacgcgt
     2941 ccttcgtcag gacctgcgtg agcgccgcga ggcccgccgc cgacgagagc gggttgccgt
     3001 tgaacgtgcc ctgctgcgcc gcgcccttgg tgacccattc catgaaccgc gcctcgcccc
     3061 cgaatgcccc gatcgtgacg ccgccgccca cggctttcgc gtacgcggcg aggtggggac
     3121 ggacgccgta gcgatcggtc gtgccgccgt acgcgatcgt ccctccgcac ttgacctcgt
     3181 cgtagatcag cgcggccccg ttgcgctcgc agatgtcccg gagcgcctgc aggtacccag
     3241 gctgcgggac gacgaggccg atgttcatca tgacgggctc caggatcacc gcagcgatct
     3301 cgcccgggtt gtcggcgaac gcctgctcca ccgcggaggc gtcgttgaac gggacgatga
     3361 tcaccgtctc ggtcaggacc ttcgggatgc ccctggacgt gggctcggtc gtgtaccagg
     3421 cccggccctc cgcgccgacg ttgaatcgca tgtcgagcac gtcggcctcg ggcaccaccg
     3481 agaacaggac ggagtcgtgg tgcccgtggt acgagccctc catcttcacg atcgtgtccc
     3541 ggccagtggc cgcccgggcc acgcggatgg catccatcgt ggcctcggtc cccgagttca
     3601 cgaaccgcat ctgctccagc tggaaccgct cgcagatcgc ctcggcgagg gcgaccgtgg
     3661 tctcggtcgt gaccgcgaag tggatgccct ggtcggcgac ggcccggatc gcctcgacga
     3721 tcttcggatg cgcgtggccc acgacgttca cgccgaaccc gccgtggaag tcgaggtact
     3781 cgttgccgtc gacgtcccag accttcgagc ccatgccgcg ccgcaggtac gtgggatagg
     3841 ggtcgccggc ctggaaattc gacgcgacgc cgttggggag gctgcgcagg gcgcgctggt
     3901 ggaggctctc ggagccgggg gtccgatcca ggagcctgcg gccctcgcgc tcggcgatct
     3961 cggcggccct ggacttcagc cgttccacat cggtcacgct gcacctcccg tcgagtgcgc
     4021 caatctaccc aggcggaccg cggtggggca acggagccgg tggtcggacc cctagggccg
     4081 gaagttcacg aactgcagcg ggacgtcgaa gtcgtggtcc ttgacggcct tcatcacgac
     4141 ctggagatcg tccttggac
//
LOCUS       DATBRK010000158         5882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_309182, whole genome shotgun sequence.
ACCESSION   DATBRK010000158 DATBRK010000000
VERSION     DATBRK010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309182"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /gene="rplJ"
                     /locus_tag="VIH70_04810"
     CDS             complement(<1..479)
                     /gene="rplJ"
                     /locus_tag="VIH70_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY8115715.1"
                     /translation="MPNAEKIAKVAELKERIQGAEALLLTEYRGLSVHDATELRRALS
                     ELARFAIVKNTLMARAAADAGVAELEALLDGPTAVAFVEGDVVSTAKKVVDAAKKFPA
                     LVIKGGYLDGKVLSAAEAQSLATLESREAMLSKVAGMLKAELTRAASMLEAIQGRFLG
                     "
     gene            complement(703..2934)
                     /locus_tag="VIH70_04815"
     CDS             complement(703..2934)
                     /locus_tag="VIH70_04815"
                     /inference="COORDINATES: protein motif:HMM:NF042625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HEY8115716.1"
                     /translation="MRESLSEREQAPPAVEPEPPRHDASPRWPLLVVGIVVLFNLVVL
                     RAEARPVQNLNDASVHRSMIAWASDRIEEGHLPLDGWYPDLALGSSRFHHYQSLPHVL
                     TGALALAIGSERALSWTMYLGMALWPIAVYAGGRLLGWDRWICAIAAAASPLIVSEPT
                     LGYEWGSYAWRGYGTWTQLWGMWLLPFAWGLSWRAVAKGRSYWLAALVLALTVACHLL
                     TGYLALLSLGVFALVEWRELLRRIGRAALIGIGSLFVAAWVVVPLLADRLWTVNDEYS
                     RGRIYYDSFGAKRVLTWFASGELFDRGRPPMITILVAVGILAALWRYRSDERARVLLF
                     LFLVALVLFFGRPTLGPVLRLLPGSGDLFLRRFVFGVHLAGLYLAGFGGVTLGRIAWN
                     QVQRFRALRERPVAIASLAVVCLVLFLAPGFIERTAWASQGAEWIGQQAVADEQGGAD
                     VAALVDIARARGPGRFYAGMRSNWGSAYEVGQVPVYVVLLNQGTEGVGFVRPTWSLSA
                     PFEYRFTDTNPTHYDLFDVRYLILEAGKPPPVQAERVAERGRHVLWERPDPGTIDLVD
                     VLPQITADRTNLGLREEDWFRSDLPAMGANPGIAFEGEPTPEATVSADDLPDTPAGTV
                     VHEDVDLRDGRASATVIAYRPAMVMLKASFDPRWQVTVDGMAVAPEMIAPSFVGRTVP
                     AGRHVVRFVYEPFPRYDLLLAIGALTLLGLGLLPGRFARRGSGGSGHGTYDPVAQPRA
                     AGT"
     gene            complement(3038..3763)
                     /gene="rplA"
                     /locus_tag="VIH70_04820"
     CDS             complement(3038..3763)
                     /gene="rplA"
                     /locus_tag="VIH70_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY8115717.1"
                     /translation="MSGGGKRYAQATELADRGREHSPVDAIRALKAFPDARFDETVEI
                     AFRLGVDPRKADQLVRGTVSLPNGSGKSVRVAVFAQGPKATEAKDAGADVVGGEELVE
                     QVMGGTIDFDAAVATPDMMAAVGKAGRVLGPRGLMPNPKTGTVTNDIAKAVEDIKGGK
                     VEYKTDRTGNVHMIIGKKSFTEQQLLENYLAVVDEIIRARPSAAKGKYVKTLTVSTTM
                     GPGVPIDTNRIKGTMEAAQTPSA"
     gene            complement(3760..4191)
                     /gene="rplK"
                     /locus_tag="VIH70_04825"
     CDS             complement(3760..4191)
                     /gene="rplK"
                     /locus_tag="VIH70_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY8115718.1"
                     /translation="MAKKKVLAVVKLQIRAGQATPAPPVGTALGQHGVNIMEFCRQYN
                     EQTQQQAGQVIPVELTIYEDRSFTFITKQPPAAELIKQAAGVEKGSGEPNREKVGRVT
                     QDQLRRIAEQKMVDLNANDVDAAMKILAGTARSMGVEVAGA"
     gene            complement(4250..5485)
                     /gene="nusG"
                     /locus_tag="VIH70_04830"
     CDS             complement(4250..5485)
                     /gene="nusG"
                     /locus_tag="VIH70_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY8115719.1"
                     /translation="MNESPIDPSDEAAPEPSTNADRDAPSDDQATREDAAADRLADAF
                     EEASAEEAVADAAIEAALEETVAEAIEDAAAVEAASDAAIEESFEERVSDAVDAGVEG
                     EALEETIAEAAADADVQESMADAAIVGALEETVAEAVEDATAVEAMSDAAILGGLADT
                     VESIAADAERAEATDDGEPAAEAEPTQDAAVEAEPTEDPEPAVDAGGGARAKKGAASR
                     AAEEPVDTAGGRDPFRGPGDWYVVHTYAGYENKVKTNLESRIHTMQMEDKIFDVHIPM
                     EDVMEIKGGKKQVVQRKVFPGYLLVKMIYDNDSWYVVRNTPGVTGFVAAGTGSKPTPL
                     SRREVEKILSVKKEEVKPQFRLGFEENDVVRIISGPFADFNGTISEINLDQSKLKVLV
                     NIFDRETPVELSFDQVAKV"
     gene            complement(5499..5789)
                     /gene="secE"
                     /locus_tag="VIH70_04835"
     CDS             complement(5499..5789)
                     /gene="secE"
                     /locus_tag="VIH70_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY8115720.1"
                     /translation="MNRQMKRAQRRQGTQVDRAQAAAATRRAQLQQKKQRTGARQFLK
                     EVRQELRKVIWPTRQELTTYTIVVLVTVVVMTSYVFGLDVLFSRLVLNVFTN"
     gene            complement(5801..5874)
                     /locus_tag="VIH70_04840"
     tRNA            complement(5801..5874)
                     /locus_tag="VIH70_04840"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5838..5840),aa:Trp,seq:cca)
BASE COUNT          860 a         2129 c         1934 g          959 t
ORIGIN      
        1 ccgaggaacc gaccctggat cgcctcgagc atcgaggccg cccgggtcag ctcggccttc
       61 agcatcccgg cgaccttcga gagcatcgcc tctcgcgact cgagggtcgc gagcgattgg
      121 gcctcggccg ccgagagcac cttgccgtcc aggtacccgc ccttgatcac cagggccggg
      181 aacttcttcg cggcgtccac gaccttcttg gcggtcgaga ccacgtcgcc ttcgacgaac
      241 gcgaccgcgg tcgggccgtc caggagcgcc tccagctcgg cgacgcccgc atcggcggcc
      301 gctctcgcca tgagcgtgtt cttgacgatc gcgaatcgcg ccagctcgga cagggcccgg
      361 cgcagctcgg ttgcgtcgtg gacggagaga ccccggtact cggtcaggag cagcgcctcg
      421 gcgccctgga tgcgctcctt gagttcggcg accttcgcga tcttctcggc gttcggcatc
      481 gaccctccat ggaacgggaa acgcgtcccc gcagacagcg gagacgcgct cgagagcgat
      541 acgtcttcgt tcgcctcggc aggtgccctt ggggattacg tgctcacgca cccctgctgt
      601 ctgcggcgaa gccaaagggt accgagccgt cggctagggc gacaaacgac gggccgccga
      661 cgaagggccg ccctcgcgat catctcgggt ccgcagggat ccctaggtcc ccgcggccct
      721 cggctgggcg acggggtcgt acgtgccgtg gccggaccca cccgatccac gtcgcgcgaa
      781 ccggccgggg agcaaaccga ggcccagcag cgtcagcgcc ccgatcgcta gtagaaggtc
      841 gtacctcggg aacggctcgt acacgaatcg gaccacgtgt cggccggccg gcacggtccg
      901 cccgacgaag ctgggcgcga tcatctcggg cgccaccgcc atgccgtcca cggtcacctg
      961 ccaacgcggg tcgaacgacg ccttcagcat caccatcgca ggccgatagg cgatcaccgt
     1021 cgcgctggcc ctcccgtccc gaaggtcgac gtcttcgtgg acgacggtac cggccggcgt
     1081 gtccgggagg tcgtcggcgc tcacggttgc ttcgggcgtg ggctccccct cgaaggcgat
     1141 gccggggttc gcgcccatgg ccgggaggtc cgagcggaac caatcctcct cgcgcagccc
     1201 gaggttcgtc cggtcggccg tgatctgcgg caggacgtcg acgagatcta tggtgccggg
     1261 gtcgggccgt tcccaaagca cgtggcgccc gcgttcggcg accctctccg cctggacggg
     1321 gggcggcttg cccgcttcga ggatcaggta tcggacgtcg aacaggtcgt agtgcgtcgg
     1381 gttcgtatcg gtgaaccggt actcgaacgg tgccgagagc gaccaggtcg gccggacgaa
     1441 cccgacgccc tcggtgccct ggttcaggag caccacgtac acgggcacct gaccgacctc
     1501 gtaggccgag ccccagttgg accgcatgcc cgcgtagaac cgacccggcc cccgtgctct
     1561 cgcgatgtcg accagggccg cgacgtcggc gccgccctgc tcgtcggcga ccgcctgctg
     1621 cccgatccat tcggcaccct gcgaggccca cgccgtgcgt tcgatgaagc cgggggcgag
     1681 gaagagcacc agacagacaa ccgccagcga cgcgatcgcc accggtcgtt cgcgcagggc
     1741 tcggaaccgt tgcacctggt tccacgcgat ccgcccgagc gtgaccccgc cgaacccggc
     1801 gaggtacaac ccggccagat gcaccccgaa cacgaaccgc cgaaggaaca ggtcgccgga
     1861 gccgggcaag agccgcagga cgggtccgag cgtcggccgc ccgaagaaga ggaccagcgc
     1921 aacgaggaac aggaacagga gcacgcgggc ccgctcgtcg ctgcggtatc gccagagcgc
     1981 cgccaggatg cccaccgcga ccaggatcgt gatcatcggc ggtcgcccac ggtcgaacag
     2041 ctcgccgctc gcgaaccagg tcaggacccg cttcgccccg aacgagtcgt agtagatcct
     2101 cccgcgcgag tactcgtcgt tgaccgtcca caaccggtcg gcgagcaacg gcacgaccac
     2161 ccacgcggcg acgaagaggc tgccgatccc gatcaatgcc gcgcggccga tcctgcgcag
     2221 gagctcccgc cactcgacga gcgcgaacac gcccagcgac agcaaggcca ggtagccggt
     2281 cagcagatga cacgcgaccg tcagggccag cacgagcgcc gcgagccagt acgaacggcc
     2341 cttcgcgacc gcacgccacg acaggcccca cgcgaacggc aacagccaca tcccccacag
     2401 ctgcgtccag gttccatagc cgcgccacgc gtagctgccc cattcgtagc cgagcgtggg
     2461 ttcgctcacg atcaacggtg aggcggccgc cgcgatcgcg cagatccatc ggtcccaacc
     2521 cagcaaccga ccgccggcgt agaccgcgat cggccacagg gccatcccga ggtacatcgt
     2581 ccacgagagc gcgcgctcgc tgccgatcgc gagcgcgagc gcgccggtca acacgtgcgg
     2641 gaggctctga tagtggtgga acctggagga tccgagcgcg agatcggggt accatccgtc
     2701 gagcgggagg tgcccctcct cgatccggtc gctcgcccac gcgatcatcg accgatgcac
     2761 cgacgcgtcg ttcagattct ggaccgggcg agcctcggcg cggagtacga ccaggttgaa
     2821 caggacgacg atgcccacca ccaggagcgg ccaacgcggg gaggcatcgt ggcggggcgg
     2881 ttcgggctcg acggccgggg gcgcctgttc gcgttcggag agggactcgc gcacccgctc
     2941 atgttgccac ggctccctcg cagcccgtta gccgggcgga gccctcgacg gcacgcgagc
     3001 cggcctaggg gtcggctcgc gtgctcgcat gtgactctca ggcgctcggc gtctgcgccg
     3061 cctccatcgt ccccttgatc ctgttcgtgt cgatcgggac acccggcccc atcgtcgtgg
     3121 agaccgtgag ggtcttcacg tacttgccct tggcggccga gggccgcgcg cggatgatct
     3181 cgtcgacgac cgcgagatag ttctcgagca actgctgctc ggtgaacgac ttcttcccga
     3241 tgatcatgtg cacgttgccc gtgcggtcgg tcttgtactc gaccttgccg cccttgatgt
     3301 cctcgacggc cttggcgatg tcgttcgtga ccgttccggt cttcgggttc ggcatgaggc
     3361 cgcgggggcc gagcacccgg ccggccttgc cgacggcggc catcatgtcc ggcgtcgcga
     3421 cggcggcgtc gaagtcgatc gtcccgccca tcacctgctc gacgagctcc tcgcctccca
     3481 cgacgtccgc gcctgcgtcc ttggcctcgg tcgccttcgg accctgggcg aacacggcca
     3541 cgcggaccga cttgcccgag ccgttcggca gcgagacggt gcctcggacg agctggtcgg
     3601 ccttgcgcgg gtcgacgccc agccggaacg cgatctcgac cgtctcgtcg aacctggcgt
     3661 ccgggaaggc cttgagggcc ctgatcgcgt cgaccggcga gtgctcgcgc ccgcggtcgg
     3721 ccagctcggt cgcctgcgcg taccgcttgc ccccgccgct catgcgcccg ccacctcgac
     3781 gcccatgctc cgggccgtcc ccgcgaggat cttcatcgcg gcgtcgacgt cgttcgcgtt
     3841 gaggtcgacc atcttctgct cggcgatccg ccgaagctgg tcctgggtga cgcgcccgac
     3901 cttctcgcgg ttgggctcgc ccgagccctt ctcgacaccg gccgcctgct tgatcagctc
     3961 cgcggccggg ggctgcttgg tgatgaacgt gaacgagcga tcctcgtaga tcgtgagctc
     4021 gaccgggatc acctggcccg cctgctgctg cgtctgctcg ttgtactgcc gacagaactc
     4081 catgatgttc acgccgtgct ggccgagcgc ggtccccacg ggtggagcgg gcgtcgcctg
     4141 acccgcgcgg atctgaagct tgacgacggc gagcaccttc ttcttcgcca tcctgggtca
     4201 cctcctgcgg ttcgaacggc ccctcggcct cccgcgtgtt cagttggtcc tagaccttcg
     4261 ccacctggtc gaaggacagt tcgaccgggg tctcccggtc gaagatgttc acgagcacct
     4321 tcagcttgct ctggtcgagg ttgatctcgc tgatcgtgcc gttgaagtcg gcgaacggtc
     4381 ccgagatgat ccgcaccaca tcgttctcct cgaatccgag gcggaactgc ggcttcacct
     4441 cctccttctt caccgacagg atcttctcca cctcacgacg cgagagcggc gtgggcttgc
     4501 ttcccgtccc ggccgcgacg aaccccgtca ccccgggcgt gttgcggacg acgtaccacg
     4561 agtcgttgtc gtagatcatc ttcacgagga ggtagccggg gaagaccttc cgctggacga
     4621 cctgcttctt gccccccttg atctccatga cgtcctccat cgggatgtgg acgtcgaaga
     4681 tcttgtcttc catctgcatg gtgtggatgc gcgattcgag gttcgtcttg accttgttct
     4741 cgtagccggc gtaggtgtgc acgacgtacc agtcgccggg gccgcggaag gggtcacgcc
     4801 cgcccgcggt gtcgacgggc tcctcggccg ctcgcgacgc ggcgcccttc ttggccctcg
     4861 cgccgccgcc ggcgtcgacc gctggttcgg ggtcctcggt gggttcggcc tcgactgcgg
     4921 cgtcttgcgt gggttcggcc tcggctgcgg gttcgccgtc gtcggtggcc tcggcccgct
     4981 cggcgtcggc cgcgatcgac tcgaccgtgt cggccaggcc gcccaggatc gcggcgtcgc
     5041 tcatggcctc gacggccgtc gcgtcctcga cggcctccgc gacggtctcc tcgagcgccc
     5101 cgacgatcgc ggcgtcggcc atcgactcct ggacatcggc gtccgccgcg gcctccgcga
     5161 tggtctcctc cagcgcctcg ccctccacgc ccgcatcgac ggcatcggac acccgctcct
     5221 cgaacgactc ctcgatcgcg gcgtcggatg cagcctcgac cgccgccgcg tcctcgatcg
     5281 cctcggccac cgtctcctcg agcgccgcct cgatcgcggc gtccgcgacg gcttcctccg
     5341 cggaggcctc ctcgaacgca tccgcgagcc ggtcggcggc ggcgtcctcc cgggtggcct
     5401 ggtcatcgga cggcgcatcg cgatccgcgt tggtggacgg ctcgggggca gcttcgtccg
     5461 aggggtcgat cggggattcg ttcatggggt gcgcgcctct agttcgtgaa gacgttcagg
     5521 accagcctcg agaagagcac gtcgagaccg aacacgtacg acgtcatgac gacgaccgtg
     5581 acgagcacga cgatcgtgta cgtcgtgagt tcctgacgag tgggccagat cactttcctc
     5641 agctcctgac ggacttcctt caggaactgg cgggcgcccg tgcgctgctt cttctgctgg
     5701 agctgcgccc gacgcgtggc cgcagcggcc tgggcacgat cgacctgggt gccctgacgg
     5761 cgctgcgccc gcttcatctg tctgttcatc gtgctccttg caggggcgga gggactcgaa
     5821 cccccaaccg ccggttttgg agaccggtgc tctaccaggt tgagctacgc ccctccgcaa
     5881 ga
//
LOCUS       DATBRK010000159         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_309279, whole genome shotgun sequence.
ACCESSION   DATBRK010000159 DATBRK010000000
VERSION     DATBRK010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309279"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VIH70_04845"
     CDS             <1..355
                     /locus_tag="VIH70_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440736.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY8115721.1"
                     /translation="IVDRLKEMILVSGFNVYPNEIEDAMYRHPKISKVAVIGVPDDKT
                     GEAVKAFVVLREGESATVEELVAWARDPANGLTAYRSPKRIELRESLPETMVGKVLRR
                     VLAEEERQQAATSAP"
     gene            352..897
                     /locus_tag="VIH70_04850"
     CDS             352..897
                     /locus_tag="VIH70_04850"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8115722.1"
                     /translation="MSTLPADLGDGAVLRRLTMADLNEIWALIEAERPRLEPWMPWVD
                     ATRTIDDQRRWLESVVHDQRSLDGCGIFVDGRYAGGVGLRWDPFGVRGEIGYWIASAQ
                     EGRGLVTRAVRALIGVGFGDLGLHRIEIRAGVDNTRSRAIPERLGFVREGIERGGERG
                     SKGFYDLVVYSILEDEWPRSP"
     gene            894..1586
                     /locus_tag="VIH70_04855"
     CDS             894..1586
                     /locus_tag="VIH70_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8115723.1"
                     /translation="MSDIEAVVFDLFGTLVYEFPRADWDGWLETSAAILEADVDAFKA
                     AWQATGIERQTGRLGDLEENLRTVAARAGAWPTDGQLTEILDGRAAMYRKWFVPRPGA
                     EEILARLRADGYRLALISMCAPDAPAMWRASPFEGTVDVEVFSCEVGLRKPEPEIYLL
                     AAERLGVRPGACLYVGDGSYRELSGAAAVGMRPVLIRDPQEEAEMLRPEVEAWHGASI
                     DDLRRIPELLEA"
     gene            complement(1583..>1846)
                     /locus_tag="VIH70_04860"
     CDS             complement(1583..>1846)
                     /locus_tag="VIH70_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115724.1"
                     /translation="ADGAVSGLATAFPEIVAALVHERDAMAGERVSTLRTLLGPIPFH
                     AALKEILVARGVPIRPAVRSPLRPLAAEEREIALDAARLVGAL"
BASE COUNT          265 a          615 c          718 g          248 t
ORIGIN      
        1 catcgtcgac cggctcaagg agatgatcct ggtctccggg ttcaacgtgt acccgaacga
       61 gatcgaggac gccatgtacc ggcacccgaa gatctccaag gtcgcggtga tcggggtgcc
      121 ggacgacaag accggggagg ccgtgaaggc gttcgtggtc cttcgcgagg gcgagtcggc
      181 cacggtcgag gagctcgtgg cgtgggcgcg cgacccggcc aacggactca ccgcgtaccg
      241 gagcccgaag cggatcgagc tgcgggagag cctgcccgag acgatggtgg gcaaggtgct
      301 ccgtcgggtg ctcgccgagg aggagcgtca gcaggccgcg acgtccgccc cgtgagcacc
      361 ctgcccgccg atctcggcga cggcgccgtc ctgcggcggc tcacgatggc ggacctgaac
      421 gagatctggg cgctgatcga ggccgagcgg cctcgcctcg aaccctggat gccgtgggtc
      481 gacgcgaccc ggacgatcga cgaccagcgg cggtggctcg agagcgtggt gcacgaccag
      541 cgatcgctcg acgggtgcgg catcttcgtg gacgggcggt acgccggcgg cgtcggactt
      601 cggtgggacc cgttcggcgt acggggggag atcgggtact ggatcgcctc cgcccaggag
      661 ggccgcgggc tcgtgacgcg ggcggtgcgc gcgctgatcg gggtgggctt cggcgacctg
      721 ggccttcacc ggatcgagat ccgggcgggc gtcgacaaca cccggagccg cgcgatcccc
      781 gagcgcctcg ggttcgtccg cgaggggatc gagcgcgggg gcgagcgcgg atcgaagggg
      841 ttctacgacc tcgtcgtgta ctcgatcctc gaggacgaat ggccgcggtc cccatgagcg
      901 acatcgaggc ggtcgtcttc gacctgttcg ggacgctcgt gtacgagttc ccccgcgcgg
      961 actgggacgg ctggctcgag acgagcgcag cgatcctgga ggccgacgtc gacgcgttca
     1021 aggccgcgtg gcaggcgacc gggatcgaac gccagaccgg ccgcctcggc gacctcgagg
     1081 agaacctgcg caccgtcgcc gctcgagcgg gcgcctggcc gaccgacggc cagctcaccg
     1141 agatcctgga cggccgggcg gcgatgtacc ggaagtggtt cgtgccgcgt cccggcgccg
     1201 aggagatcct cgctcggctg cgggccgacg gctaccggct cgcgttgatc agcatgtgcg
     1261 cgcccgacgc accggcgatg tggcgcgcgt cgccgttcga ggggaccgtc gacgtcgagg
     1321 tgttctcgtg cgaggtcggg ctccgcaagc ccgaacccga gatctacctg ctcgcggccg
     1381 agcgtctcgg cgtaaggccg ggggcctgcc tgtacgtcgg cgacggctcc taccgcgagc
     1441 tctcgggcgc ggcggccgtc gggatgcgac cggtgctgat ccgcgatccg caggaggagg
     1501 cggagatgct ccgccccgag gtcgaggcgt ggcacggcgc gtccatcgac gacctgcgcc
     1561 ggatccccga gctgctcgaa gcctagagcg cgccgacgag gcgagcggcg tcgagcgcga
     1621 tctcgcgttc ctcggcggcg agcggccgca gcggggatcg cacggcgggc cggatcggga
     1681 cgccgcgggc gaccaagatc tccttgagcg cggcgtggaa cgggatcggg ccgaggagcg
     1741 ttcgcagcgt cgagacccgt tcgcccgcca tggcgtcccg ctcgtgcacg agcgcggcca
     1801 cgatctcggg gaacgccgtc gcgaggcccg agaccgcgcc gtcggc
//
LOCUS       DATBRK010000160         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_24173, whole genome shotgun sequence.
ACCESSION   DATBRK010000160 DATBRK010000000
VERSION     DATBRK010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24173"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VIH70_04865"
     CDS             complement(<1..203)
                     /locus_tag="VIH70_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter"
                     /protein_id="HEY8115725.1"
                     /translation="MSTQTLERPAAPPPSPPGAGPRPSVAIRSALARWGLRGTALLYL
                     GLIVALPTVAVITRGFQDGLQSLR"
     gene            complement(208..1296)
                     /locus_tag="VIH70_04870"
     CDS             complement(208..1296)
                     /locus_tag="VIH70_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8115726.1"
                     /translation="MRSRLAFRLSIVVAVFGLIAAACSSDDSGTVSGTSPVASDCTPA
                     ESPIVTVTAYSNVYDVYGKLISTFQSAWKESHNDQQIQFSTSFGGSTTQAQNVIAGFP
                     ADVAALSLYPDIQLIEDAGLITHDPTQDPEAGIVASSAVVFDVRPGNPLNIDNWDDLA
                     KPGVEILTPDPGSSGGARWNIVAAYGAAMRGEVPGYPANDPAAAQRLLTDIFENVTVM
                     DKSANDSIKNFQAGNGDVAITYEYAVLAAQNSGLEDEMVIPPSTVAIQTPVSVIDANA
                     EAHCVQDVAAAFVDFLHSDEAKELFLTVGFERSVDLKAAQAGDDGAYQPIDDLFTTDD
                     IGGWDELQNDTVFGPDGAFTKALAAAQG"
     gene            complement(1476..1646)
                     /locus_tag="VIH70_04875"
     CDS             complement(1476..1646)
                     /locus_tag="VIH70_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HEY8115727.1"
                     /translation="MAEDHADDLAHLTDEERAVLMKVVEVLRKLRFGTVLLVVQDGKV
                     VQIEMAEKIRLR"
     gene            1744..2112
                     /locus_tag="VIH70_04880"
     CDS             1744..2112
                     /locus_tag="VIH70_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115728.1"
                     /translation="MSGTNEAWNEVGERFASWGRHVAERYKESSGSARESAQESQRKL
                     EDAARDITNALDRAFTALGDTIRDERAKDDLKQAVRALGDAVSVTVAETTERIRHGVG
                     PSSPDPTGDSKDDEPGPTSA"
     gene            complement(2125..>2263)
                     /locus_tag="VIH70_04885"
     CDS             complement(2125..>2263)
                     /locus_tag="VIH70_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004461993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8115729.1"
                     /translation="IGPRIPEQLEGAMRALDLKLDGAAMKRLEEIWPGPGGAAPEAFA
                     W"
BASE COUNT          357 a          741 c          802 g          363 t
ORIGIN      
        1 cggagggact ggaggccgtc ctggaatccg cgggtgatca ccgccacggt cggcagggcg
       61 acgatcaatc cgaggtagag cagcgccgtc ccccgcagcc cccagcgcgc gagtgcgctc
      121 cggatcgcga ccgacggacg cggccccgcc cccggcggag acggtggcgg ggcggcgggg
      181 cgttccagtg tctgtgtgct caaagcgcta cccctgtgcg gcggccaggg ccttggtgaa
      241 ggcgccgtcc ggtccgaaca ccgtgtcgtt ctgcagctcg tcccagccgc cgatgtcgtc
      301 ggtcgtgaac aggtcgtcga tcggctggta cgcgccgtcg tcaccggcct gcgccgcctt
      361 cagatcgacc gaccgctcga acccgacggt caggaagagc tccttcgcct cgtccgagtg
      421 caggaagtcc acgaacgcgg cggcgacgtc ctgcacgcag tgcgcctcgg cgttcgcgtc
      481 gatcacactg accggtgtct ggatcgcgac ggtcgagggc gggatcacca tctcgtcttc
      541 cagaccggag ttctgggcgg cgaggaccgc gtactcgtag gtgatcgcga cgtcgccgtt
      601 gcccgcctgg aagttcttga tggagtcgtt cgcgctcttg tccatcaccg tgacgttctc
      661 gaagatatcg gtgagtaggc gctgcgcggc cgccgggtcg ttggccgggt acccggggac
      721 ctctccccgc atcgccgcgc cgtaggcggc cacgatgttc cacctcgcgc cgccgctcga
      781 gccggggtcg ggcgtcagga tctcgacgcc cggcttggcg aggtcgtccc agttgtcgat
      841 gttcagtggg ttgcccggcc gaacgtcgaa cacgacggcc gatgaggcca cgatgccggc
      901 ctcgggatcc tgcgtggggt cgtgagtgat gagtcctgcg tcctcgatca gctggatgtc
      961 cgggtacagc gacagcgccg ccacatcggc ggggaagccg gcgatcacgt tctgcgcctg
     1021 cgtcgtgctg ccgccgaacg aggtcgagaa ttggatctgc tggtcgttgt gcgattcctt
     1081 ccaggccgac tggaaggtcg agatcagctt gccgtacacg tcgtacacgt tgctgtacgc
     1141 agtgacggtg acgatcggcg actccgctgg ggtgcagtcg gaggcgaccg gggacgtgcc
     1201 cgagacggtg ccggagtcgt cgctcgagca agcggcggcg atcaggccga agaccgcgac
     1261 cacgattgac agtcggaagg cgagacgaga ccgcaagggt gtgctccttt cgagtggacc
     1321 ggttggaagt cgaactacgg ctcgatctcc ggtggtccgg tcgatcgaaa ggacgcaacg
     1381 tcgcgggaca cggcctccgt gggtacggtc agccgaacgg gctatggata tcaatgcccc
     1441 ccaggccctg tcaaccaacc tgtgcggttc ggtgcctacc ggaggcggat cttctcggcc
     1501 atctcgatct ggacgacctt gccgtcctgg acgaccagga ggacggtccc gaacctcagc
     1561 tttcgaagga cctcgacgac tttcatcagg accgcacgct cctcgtccgt gagatgcgcg
     1621 aggtcatcgg catggtcctc ggccatggcg ggcaaacctt accccagggg cgttcgggga
     1681 cgaccctcct tcacgtgcag ggcctagact cgggtcggca ccggatcgga cggagggatc
     1741 gcgatgtccg gcacgaacga ggcatggaac gaggtcggcg aacggttcgc gtcgtggggg
     1801 cgccacgtcg ccgagcggta caaggagtcg agcggttccg ctcgcgagtc ggcgcaggag
     1861 agccagcgca agctggagga cgccgcgcga gacatcacca atgcgctcga tcgggccttc
     1921 acggcgctcg gggacacgat ccgggacgag cgggccaagg atgatctgaa gcaggcggtc
     1981 cgtgcgctgg gcgacgccgt ctcggtcacc gtggccgaga cgaccgaacg gatccgacat
     2041 ggcgtcggcc cgtcgagccc ggatccgacc ggcgattcga aggacgacga gcccgggccg
     2101 acctcggcgt agcatccggc gccgctacca ggcgaacgct tcgggcgcgg ccccgcccgg
     2161 acccggccag atctcctcga gacgcttcat cgcggcgccg tcgagcttca ggtcgagcgc
     2221 ccgcatcgcg ccctcgagct gctcggggat ccgcgggccg atg
//
LOCUS       DATBRK010000161         9008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2481281, whole genome shotgun sequence.
ACCESSION   DATBRK010000161 DATBRK010000000
VERSION     DATBRK010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9008
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481281"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VIH70_04890"
     CDS             <1..1090
                     /locus_tag="VIH70_04890"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8115730.1"
                     /translation="TGSSVWLAAALIVTEGVTGLVGPLASVLGDRFDRRTVMIVSDLA
                     AAACFAGMALVASSPGPLVAIAFLSALTEAPFWPASSAAIPNLVPQDQVSWANSLVAI
                     GRNVGIMVGPAVGGILLAAVGASWVFAINAASFAVSAALVVSVRARFVGDRSDEGEHR
                     GVWAGFRFLFHEPVLRRISVAWIVLVLGIGMAMVADVPLVDVFGAGSAGFGVMIGLWG
                     AGSILGSLAGRWLTAATETRWLVLGTALTACIGIAIALSPWFALVLVLNVAWGAGEGI
                     TGVAEQNLFQRRSPDSVRSRVLGAVEGLFHGGLTVSFAAAAFVLPVVGARGMYAIAGV
                     AAGLAAIVLLPLLGETPITAGQPTAELP"
     gene            1205..2788
                     /locus_tag="VIH70_04895"
     CDS             1205..2788
                     /locus_tag="VIH70_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8115731.1"
                     /translation="MIGGGHNGLVAAAYLARAGARVVVCEARHKTGGAAATDQPWPDH
                     PEFRVTTLSYVMSLMPDTIVRDLQLARHGYRVHPVGPYVVPFPDGRVMVQYNDPSKNR
                     DEFAKFSRHDADALEHWEAWIGALAAVLGPLLLSTPPQIGSKRPADLLEQLRLAWRLR
                     GLSVRTVGEVTRLLTMSVADLLDRFFESEQVKAVMAIDGLIGTWAGPREPGTAYVMAH
                     HEIGDVGDGTLGSWGVPEGGMGAVAAALERSARAFGAEVRIGAAVDRVLVAGGRARGV
                     VLAGGEELLAPVVVSACHPMITFLRQLDRRDLPADFVQDIEHWSTRSGVVKVNCAISR
                     LPTLAGEPDWRDFSGGFEIAPSVDQMERSFEEARAGRPATLPFSDGVIPTTLDPSLAP
                     EGAHVISLFTQWVPHTWSEEPHREELEAYADRVVRAYDDVAPGFRESIVARQVLGPYD
                     METEWNLIGGKIFHGELSADQLFHMRPAPGYADYRTPIDGLYQCSSATHGGGGVSGIP
                     AYNCVREIRRDRKRRSGRR"
     gene            2785..3930
                     /locus_tag="VIH70_04900"
     CDS             2785..3930
                     /locus_tag="VIH70_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY8115732.1"
                     /translation="MKEPYSFTFYTASWYRRSPYWQRTVEAGCTSWDLYNHMLIPTLY
                     DDDEREYWHLVEHVGLWDVAVERQVEIVGPDAVRFTQLLTCRDLSRCDVLQCKYAPII
                     APDGGIVNDPILLRLGENHFWLSLADSDAMLYALGVQAFAGMDVTIREPDVSPLQVQG
                     PKSGDVIRALFGNEVADLGYYRCWEGDLGSIPVVVSRTGWTGEVGYEIYLRDGSRGTE
                     LWDRVMEAGAPSQIRPIAPSDQRRLEAGIFNYGNDMTVRDTPFHVTGMERLVEFDADF
                     IGRDALRRVAAEGVDRKLVGVTLGGEPFRMWLEDFWVVRRDGRDVGRLVSASHSFRRK
                     ENIGYAWVPIELAEPGNELEVLSPDGPVPAVTAALPFLDPNKEIPKG"
     gene            3951..4493
                     /locus_tag="VIH70_04905"
     CDS             3951..4493
                     /locus_tag="VIH70_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY8115733.1"
                     /translation="MDPLSGHLLIAGTSLLDPNFRRAVVLIGHHDDEGAVGVVLNRAS
                     EVEVAVAVPPLAGLVEPGDRLFIGGPVSSQSAVVVADFEHPEVADVVAFDSVGFLPEE
                     TDAASIGGLRRARVFAGYAGWRAGQLETELDEGSWITEPALASDVFTEDPDALWADVL
                     RRKGPEYRLMSTMPFDPDLN"
     gene            4550..5701
                     /locus_tag="VIH70_04910"
     CDS             4550..5701
                     /locus_tag="VIH70_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY8115734.1"
                     /translation="MASETFTFYIQPWYRKSPYFEATKRHGCKSWGLYNHMLLPTLYD
                     DPVTEYEALLHDVTLWDVAVERCVEVRGPDAFDLANLVTCRDLTRVEPMQARYVLLIA
                     AWGGIVNDPVLLRLDRNRFWLALADSDALLYLAGVAGAREMDVSIFEADVAPMQIQGP
                     RSKSVMRDLFGDAVADLRYYFCAEADVDGIPVVISRTGWSGEVGFELYLQDTSRGDEL
                     FERVYQAGEPHGIRVVAPSEARRIEAGIFNYGSDIRVEDTPLHVSGLERFVEWDQDFI
                     GRARLEEIRDGDLIDRKLVGIDIGGEPMRDEGALNDFWPLVKGDERLGRVTAGAWSPR
                     LERNIGYAWVPIRWEAPGSTFEAETPRGRVPVTVASLPFVDPSKDIPKS"
     gene            5713..6498
                     /locus_tag="VIH70_04915"
     CDS             5713..6498
                     /locus_tag="VIH70_04915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8115735.1"
                     /translation="MSGSIRFDGAADHYDRTRAISNEAMGRTISLLASELRDRGRVLE
                     VGVGTGLLALRLHEAGIPVSGLDLSAPMLAKLVEKAGGAAPFPLVLADATTIPFADGA
                     FGAAYLRWVLHLIPDWRTVLAEMARVVRPGGVLVVSLGAFDEVGRAVRARFSEITGLS
                     TDPVGLMWANHDALDAELERLGLRLRVLPAIPEEEEEPLASFLDAIEEGRWSWTWHVP
                     KDTRVDAVRELRPWAEERFGPLDRIERYEVETVWRAYEVPGQG"
     gene            6605..7531
                     /locus_tag="VIH70_04920"
     CDS             6605..7531
                     /locus_tag="VIH70_04920"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY8115736.1"
                     /translation="MPITEVEKIWKDGELVDWADANVHVLSHAVHYGSGVFEGIRAYE
                     TARGPAVWHLDEHLKRLFRSAKLYHMDIPFSIEAITDGIKDLIRANELAACYIRPIVL
                     RGYGEMGVNSLNAPVNVFIAVWPWGAYLGEDALENGVRIKIASWRRNSQNALPSSAKA
                     TGQYINGVLAKIESIKAGYDEAVMLNEQGFITDGSGENLFIVRERRLTTPPISAGCLD
                     GITRGTVIALARDLGYEVTEENLVRTDLYNADECFFTGTAAEITPIREVDDRAVGEGH
                     RGPITKELQGTFFAATKGETERHASWLTYVND"
     gene            7536..>9008
                     /locus_tag="VIH70_04925"
     CDS             7536..>9008
                     /locus_tag="VIH70_04925"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY8115737.1"
                     /translation="MTDQPTIGERVDDDEARYRLPRTVFPHRYDLVLEPDLEATTFAG
                     SETVTLEVVEPVDEIVLNAIDLDVGPGRLEGLGGTLEISTIRLDPESERAHLLLSGTA
                     EPGVWTLHLEFRGKLSDQLQGFYRSTYTDDDGRTRTIATTQFESTDARRAFPCWDEPD
                     LKAVFGVTLVVDDDVLAVSNGPEIGRESAGDGRVRVRFADTMEMSTYLVAFVVGPLEA
                     THPVDVDGVPLRIVYRPGKAGLSAFAAEVGAFSLRFFADYYAIPYPDAKMDMVGLPDF
                     AQGAMENLGCVTYRESLLLVDPDNATQPELATVADVIAHELAHMWFGDLVTMRWWNGI
                     WLNEAFATLMELLAVDAYRPDWERWSQFLRSRAIAFEVDALQSTRSIEYPVRSPDDAS
                     GMFDILTYTKGAAVLRMLEQYLGAERFRDGIRRYLDEHRFGNTETHDLWDAIEKATGE
                     PVRRIMDGWIWQGGYPLVSARLRNGTVELGQRRYLISGEED"
BASE COUNT         1292 a         3028 c         3279 g         1409 t
ORIGIN      
        1 gacggggtct tcggtctggc tcgcggcggc gctgatcgtc accgagggcg tgaccgggct
       61 ggtcggtccg ctcgccagcg tgctggggga ccggttcgac cgccggacgg tcatgatcgt
      121 gtcggatctg gcggcggcgg cgtgcttcgc ggggatggcg ctggtcgcct cgtcgcccgg
      181 tcctctggtt gcgatcgcct tcctgtcggc gctgaccgag gcgccgttct ggcccgcctc
      241 gagcgccgcc atcccgaacc tggtcccgca ggaccaggtc agctgggcga acagcctggt
      301 cgcgatcggg cgcaacgtcg gcatcatggt cgggccggcg gtcgggggga tcctgctcgc
      361 cgccgtcggc gcatcgtggg tgttcgcgat caacgccgcg tcgttcgcgg tctcggccgc
      421 gctggtggtc tcggtgcgcg ctcgcttcgt cggcgaccga tcggacgagg gcgagcatcg
      481 cggcgtgtgg gcgggcttcc ggttcctgtt ccacgagccg gtgttgcgac ggatctcggt
      541 ggcctggatc gtgcttgtcc tgggcatcgg gatggcgatg gtcgccgacg tgcctctggt
      601 cgacgtgttc ggcgcggggt cggccgggtt cggggtgatg atcgggctct ggggtgccgg
      661 gtcgatcctc ggttccctcg cggggcgctg gctcacggcg gccaccgaga cgcgctggct
      721 ggtcctgggg accgcgttga ccgcgtgcat cggcatcgcg atcgcgctct cgccgtggtt
      781 cgccctcgtc ctggtcctga acgtcgcctg gggagccggc gaggggatca ccggcgtcgc
      841 cgagcagaac ctgttccagc gtcgatcgcc ggactcggtg cgcagccggg tcctgggcgc
      901 cgtcgagggt ctgttccacg gcgggctgac ggtctcgttc gcggcggcgg cgttcgtgct
      961 gcccgtggtg ggggcgcgag ggatgtacgc gatcgccggc gtggccgcgg gcctggcggc
     1021 gatcgtgctc ctcccgcttc tcggcgagac gcccatcacc gccggtcaac cgaccgccga
     1081 gctcccctga ggatcgcgcc gcctcgcgtg ggcgggcctc cccggggtaa cgtttccgag
     1141 ctgaaccgtc gttcaaggtc caagaggagg agcgctggcg gatccgaacg ccttcgacgc
     1201 gatcgtgatc ggcggcggcc acaacggcct ggtcgccgcc gcgtacctcg cccgcgccgg
     1261 cgcccgggtc gtcgtatgcg aggcgcgtca caagaccggt ggcgctgccg cgaccgatca
     1321 gccctggccc gatcacccgg agttccgcgt cacgacgctc tcgtacgtga tgagcctgat
     1381 gccggacaca atcgtccgcg acctgcaact cgcgcggcac gggtaccgcg tgcacccggt
     1441 cggtccctac gtcgtgccgt tcccagacgg ccgggtgatg gtccagtaca acgacccgtc
     1501 caagaaccgc gacgagttcg ccaagttctc caggcatgac gccgacgcgc ttgagcattg
     1561 ggaggcgtgg atcggagccc tcgccgccgt cctcggaccg ctgctgttga gcacgccgcc
     1621 gcagatcggt tccaagcggc ccgccgacct gctcgagcag ctccgcctgg cctggcggct
     1681 ccgggggctg tcggttcgca cggtcggcga ggtgacgcgg ctcctgacga tgagcgtcgc
     1741 cgacctgctc gaccggttct tcgagtccga gcaggtcaag gccgtgatgg cgatcgacgg
     1801 cctgatcggg acgtgggcgg gtccccgcga gccggggacc gcctacgtaa tggcgcatca
     1861 cgagatcggc gacgtcggcg acgggactct tggctcgtgg ggcgtccccg agggcgggat
     1921 gggcgccgtg gccgcggcgc tcgagcgcag cgcgcgcgcg ttcggggccg aggtgcgcat
     1981 cggggcggcg gtggaccggg tcctggtcgc cggcggacgc gctcgcggcg ttgtccttgc
     2041 gggcggggag gagctgcttg cccccgtcgt cgtctcggcg tgccacccga tgatcacgtt
     2101 cctccggcag ctcgaccgca gggacctgcc ggcggacttc gtccaggaca tcgagcactg
     2161 gtcgacgcgc tcgggcgtcg tcaaggtcaa ctgcgcgatc agccggctgc cgacgctggc
     2221 cggcgaaccg gactggaggg acttctcggg cgggttcgag atcgcgccct ccgtcgacca
     2281 gatggagcgg tcgttcgagg aggcccgggc ggggcggccc gccacgctgc cgttctccga
     2341 cggcgtgatc ccgactacgc tggatccgtc gctcgcgccc gagggtgcgc acgtgatctc
     2401 gctgttcacg cagtgggtcc cgcacacgtg gtcggaggag ccgcatcgag aggagctcga
     2461 ggcttacgcc gaccgcgtcg tccgggcgta cgacgacgtc gcgccggggt tcagggagtc
     2521 gatcgtcgcc cggcaggtgc tcgggccgta cgacatggag acggagtgga acctgatcgg
     2581 cggcaaaatc ttccacggcg agctgtcggc cgatcagctg ttccacatgc gtcccgcgcc
     2641 cggctacgcc gattatcgga cgccgatcga cggcctgtat caatgctcga gcgccacgca
     2701 cgggggcggg ggcgtgtccg gcatccccgc gtacaactgc gtgcgcgaga tccgcaggga
     2761 tcggaagcgc cggagcgggc gtcgatgaag gagccgtact cgttcacgtt ctacaccgcc
     2821 tcgtggtacc ggcgttcgcc gtactggcag cgaaccgtcg aggccggctg cacgagctgg
     2881 gacctgtaca accacatgct gatcccgacc ctgtacgacg acgacgagcg ggagtactgg
     2941 cacctggtcg agcacgtcgg gctgtgggac gtggccgtcg agcgtcaggt cgagatcgtc
     3001 ggacccgacg ccgtgaggtt cacgcagctc ctcacgtgcc gggacctgtc gcgctgcgac
     3061 gtgctgcagt gcaagtacgc gccgatcatc gcccccgacg gcgggatcgt gaacgatccg
     3121 atcctgctgc gtctgggcga gaaccacttc tggctctcgc tcgccgactc cgacgcgatg
     3181 ctgtacgcgc tcggcgtgca ggcgttcgcc gggatggacg tgaccatccg cgaacccgac
     3241 gtctcgccgc ttcaggtcca gggtcccaag agcggggacg tgatccgggc gctgttcggg
     3301 aacgaggtcg cggacctggg gtactaccgg tgttgggagg gcgacctggg ttcgatcccg
     3361 gtggtcgtct cgcgcacggg gtggacgggt gaggtcggct acgagatcta cctgcgcgac
     3421 ggctcgcgcg gcaccgagct ctgggaccgc gtgatggagg cgggagctcc atctcagatc
     3481 cgcccgatcg cccccagcga ccagcggcgg ctcgaggcgg ggatcttcaa ctacggcaac
     3541 gacatgacgg tgcgggacac gccgttccac gtgaccggca tggagcgcct ggtcgagttc
     3601 gacgccgact tcatcggcag ggatgcgctc cgacgggtgg cggccgaggg cgtcgaccgc
     3661 aagctggtcg gcgtgacgct cggcggcgag cccttccgga tgtggctcga ggacttctgg
     3721 gtcgtccgga gggacggccg ggacgtcggc cgtctcgtga gcgcctcgca ttccttccgc
     3781 cggaaggaga acatcggcta cgcgtgggtc ccgatcgagc tcgccgagcc cgggaacgag
     3841 ctcgaggtgt tgtcgcccga cggcccggtc cccgcggtca cggcggcgct gcccttcctg
     3901 gatccgaaca aggagatccc caagggttga ccgagcgaga tgatggcgcc atggacccgc
     3961 tgagcggaca cctgctgatc gccggcacgt cgctgctgga cccgaacttc cgtcgcgccg
     4021 tggtgctgat cggccaccac gacgacgagg gcgcggtggg agtcgtcctc aaccgcgcga
     4081 gcgaggtcga ggtcgccgtc gcggtgccgc ccctcgccgg cctggtggag ccgggcgacc
     4141 gcttgttcat cggcggtccg gtctcgtcgc agtcggcggt ggtcgtggcc gatttcgagc
     4201 acccggaggt ggcggatgtg gtcgcgttcg actcggtcgg gttcctgccc gaggagaccg
     4261 acgcggcgtc gatcgggggc ctccggcgcg ctcgggtgtt cgccgggtac gcgggatggc
     4321 gcgccgggca gctcgagacc gagctggacg agggctcgtg gatcaccgag cccgccctgg
     4381 cgagcgacgt cttcaccgag gaccccgacg cgttgtgggc ggacgtcctg cgccgcaagg
     4441 gtcccgagta ccggctgatg tcgacgatgc cgttcgaccc agacctcaac tagcggctag
     4501 gctcgcacct catccgccgc aggaacggcc aaggaacgca ggaggatcga tggcgtccga
     4561 gacgttcacg ttctacatcc agccgtggta ccggaagtcg ccctacttcg aggcgaccaa
     4621 gcgccacggg tgcaagagct ggggcctgta caaccacatg ctgctgccga ccctgtacga
     4681 cgaccccgtc accgaatacg aggcgctgct gcacgacgtc acgctctggg acgtcgcggt
     4741 cgagcggtgc gtcgaggtcc gcggtcccga cgcgttcgac ctcgcgaacc tggtcacctg
     4801 ccgcgacctc acgagggtcg agccgatgca ggcgcggtac gtgctgctca tcgcggcgtg
     4861 gggcggcatc gtcaacgatc ccgtgctgct tcgcctggac cggaaccggt tctggctggc
     4921 gctcgccgac tcggacgcgc tcctgtacct ggccggcgtc gcgggcgcgc gcgagatgga
     4981 cgtctcgatc ttcgaggccg acgtcgcgcc gatgcagatc cagggaccgc gctcgaagtc
     5041 cgtgatgcgc gacctgttcg gcgacgcggt ggccgacctc cgctactact tctgcgccga
     5101 ggcggacgtc gacggcatcc ctgtcgtgat cagccggacc gggtggtccg gcgaggtcgg
     5161 gttcgagctg tacctccagg acacgagccg cggcgacgag ctgttcgagc gcgtgtacca
     5221 ggccggcgag ccgcacggga tccgggtggt cgccccgagc gaggcgcggc ggatcgaggc
     5281 ggggatcttc aactacggct cggacatccg ggtcgaggac actcccctgc acgtgagcgg
     5341 gctcgagagg ttcgtcgagt gggaccagga cttcatcggg cgcgcccggc tggaggagat
     5401 ccgcgacggc gacctgatcg accgcaagct cgttggtatc gacatcggcg gcgagccgat
     5461 gcgggacgag ggcgcgctga acgacttctg gccgctggtc aagggcgacg agaggctcgg
     5521 gagggtgacg gcgggggcgt ggtctccccg gctcgagcgg aacatcggct acgcgtgggt
     5581 gccgatccgt tgggaggcgc ccggctcgac gttcgaggcc gagacgcctc gcgggcgcgt
     5641 gccggtgact gtggcgagcc tgcccttcgt cgacccgagc aaggacattc ccaagtcgta
     5701 gcctcggtgg tcatgagcgg gtccatccgg ttcgacggcg cggccgacca ctacgaccgg
     5761 acgcgcgcga tctcgaacga ggcgatgggg cgaacgatct cgctgctggc ctcggagctc
     5821 cgggaccggg gccgggtgct cgaggtcggg gtcgggaccg gtctgctcgc gctccggctg
     5881 cacgaggccg gtatcccggt ctcgggcctg gacctgtcgg ccccgatgct cgcgaagctc
     5941 gtcgagaagg cgggcggcgc ggcgccgttc cccctcgtcc tcgccgacgc cacgaccatc
     6001 ccgttcgccg atggggcgtt cggcgccgcc tacctgcggt gggtgctgca tctgatcccc
     6061 gactggcgca cggtgctggc cgagatggca cgggtggtgc gtcccggggg cgtcctggtc
     6121 gtgtccctcg gggcgttcga cgaggtcggt agggcggtcc gggcccggtt ctccgagatc
     6181 accggactct cgaccgaccc cgtcggcctg atgtgggcca accacgacgc gctcgacgcc
     6241 gagctcgaga ggttgggcct ccggctccgc gtccttcccg cgatccccga ggaagaggag
     6301 gagccgctcg cctcgttcct cgacgccatc gaggagggtc gctggtcatg gacctggcac
     6361 gtccccaagg acacgcgcgt cgacgccgtc cgcgagcttc ggccctgggc cgaggagcgc
     6421 ttcgggccgc tggaccgtat cgaacggtac gaggtcgaga ccgtgtggcg cgcctacgag
     6481 gtgcccggcc aaggctgaac ccattcccct gaccagcagc tatcctgtcg acaccggggc
     6541 cgaaccacgg aaacacaccg agaccacgac ccggaccacc gactgctccg aggaggcttc
     6601 cgagatgccg atcaccgagg tcgagaagat ctggaaggac ggcgagctcg tcgactgggc
     6661 cgacgcgaac gtccacgtac tctcccacgc cgtgcactac ggcagcgggg tgttcgaggg
     6721 gatccgagcg tacgagaccg cccggggccc ggcggtctgg cacctcgacg agcacctgaa
     6781 gcgcctgttc cgctcggcga agctgtacca catggacatc ccgttctcga tcgaggcgat
     6841 cacggacggg atcaaagacc tcatccgtgc caacgagctg gccgcgtgct acatccggcc
     6901 gatcgtgctg cgcggctacg gcgagatggg cgtcaactcg ctgaacgcgc cggtgaacgt
     6961 gttcatcgcg gtctggccct ggggcgcgta cctgggtgag gatgccctcg agaacggcgt
     7021 ccggatcaag atcgcgtcct ggcgccggaa ctcgcagaac gcgctcccgt cctccgcgaa
     7081 ggccaccggc cagtacatca acggcgtgct cgccaagatc gagagcatca aggccggcta
     7141 cgacgaggcc gtgatgctga acgaacaagg attcatcacc gacggttcgg gggaaaacct
     7201 gttcatcgtg cgggaacgca ggctcaccac gccgccgatc tccgccggat gcctggacgg
     7261 catcacgcgc gggaccgtga tcgcgctggc ccgcgacctc ggctacgagg tcaccgagga
     7321 gaacctcgtc cgaaccgatc tgtacaacgc cgacgagtgc ttcttcacgg gcacggcggc
     7381 cgagatcacg ccgatccgcg aggtcgacga ccgagccgtg ggcgaggggc accgcggtcc
     7441 gatcaccaag gagctgcagg ggacgttctt cgccgccacc aagggcgaga ccgagcgaca
     7501 cgcctcctgg ctcacctacg tgaacgactg agcgtgtgac cgaccagccc acgatcggag
     7561 agcgcgtgga cgacgacgag gctcgctacc gcctgccccg caccgtcttt ccccatcgct
     7621 acgacctggt cctcgaaccc gatctggagg cgaccacgtt cgccggctcc gagaccgtga
     7681 cgctggaggt cgtggaaccg gtcgacgaga tcgtgctgaa cgcgatcgac ctggacgtcg
     7741 gaccggggcg gctcgagggc ctgggcggga cgctcgagat ctcgacgatc cgcctggacc
     7801 cggagagcga acgagcgcac ctgctcctgt cggggacggc cgagccgggg gtctggacgc
     7861 tgcacctgga gttccgcggg aagctctcgg accagctgca ggggttctac cggtcgacgt
     7921 acaccgacga cgacggccga acgcgcacga tcgcgaccac gcagttcgag tccaccgacg
     7981 cgcgccgggc gttcccgtgc tgggacgagc ccgatctcaa ggccgtgttc ggcgtcaccc
     8041 tggtggtgga cgacgacgtg ctcgcggtct cgaacggtcc cgagatcggc cgcgaatcgg
     8101 cgggcgacgg ccgcgtccgg gtgcggttcg ccgacacgat ggagatgtcc acgtacctgg
     8161 tggcgttcgt ggtcggtccg ctcgaggcca cccacccggt cgacgtcgac ggcgtgcccc
     8221 tccggatcgt gtaccggccg ggcaaggccg gcctgagcgc cttcgcggcc gaggtcgggg
     8281 cgttcagcct tcggttcttc gccgactact acgcgatccc gtatcccgac gcgaagatgg
     8341 acatggtcgg gctcccggat ttcgcgcagg gcgcgatgga gaacctcggg tgcgtcacct
     8401 accgcgagag cttgctgctg gtcgatccgg acaacgcgac ccagccggag ctcgccacgg
     8461 tcgccgacgt catcgcgcac gagctcgctc acatgtggtt cggcgacctg gtgacgatgc
     8521 gctggtggaa cggcatctgg ctgaacgagg ccttcgccac cctgatggag ctgctcgcgg
     8581 tggacgcgta ccgccccgac tgggagcggt ggagccagtt cctgcgctcc cgcgcgatcg
     8641 cgttcgaggt ggacgcgctc cagagcacgc ggtcgatcga gtacccggtg cgctcgcccg
     8701 acgacgcatc cgggatgttc gacatcctga cgtacacgaa gggcgccgcg gtcctgcgga
     8761 tgctcgagca gtacctgggc gccgaacgct tccgcgacgg gatccggcgc tacctggacg
     8821 agcaccggtt cggcaacacc gagacgcacg acctgtggga cgccatcgag aaggccaccg
     8881 gcgagccggt ccgcaggatc atggacggct ggatctggca gggcgggtac ccgctggtct
     8941 cggcgcggct ccgcaacggc acggtcgagc tcggccagcg gcggtacctg atctcgggcg
     9001 aggaggac
//
LOCUS       DATBRK010000162         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1034662, whole genome shotgun sequence.
ACCESSION   DATBRK010000162 DATBRK010000000
VERSION     DATBRK010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034662"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..847)
                     /locus_tag="VIH70_04930"
     CDS             complement(110..847)
                     /locus_tag="VIH70_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8115738.1"
                     /translation="MRTPVVGGRRAVLEAVRAGAAREVLVGRDARSTAGLRDVLETCR
                     EAGIPVREVDRTELDGLAADHRGVAAITGTPKPMGERELAAWPFGPDDLVVVLDGITD
                     PQNLGAAARSAEAAGARMLVTRTRRAADVTPAAIRASAGALLHLTQARIANIARGLER
                     LKDVGFTVVGLDEHADRTVYEAESPEGRVAVVLGSEGEGLGRLTRAACDLLVRLPMRG
                     RVGSLNASASLAAALYAFVLPRRLGGA"
     gene            complement(844..1320)
                     /gene="ispF"
                     /locus_tag="VIH70_04935"
     CDS             complement(844..1320)
                     /gene="ispF"
                     /locus_tag="VIH70_04935"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY8115739.1"
                     /translation="MSEPLVGMGFDVHAFEAGRPLMLGGVRLDAPEGLAGHSDGDVVC
                     HALADALLGAVALGDVGEHLPETDPAFAGIGGLDLLGRVVGMVRTAGAYPVSADLTVI
                     AERPSIAPVRDAMRRALCGTLGVPTERVSVKATRPEGLGLTGDGVGCLALVVLARS"
     gene            complement(1317..1979)
                     /gene="ispD"
                     /locus_tag="VIH70_04940"
     CDS             complement(1317..1979)
                     /gene="ispD"
                     /locus_tag="VIH70_04940"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY8115740.1"
                     /translation="MDRAVAIVLAAGRGERMDTTLPKALMPFGGATIVARAVELASAA
                     AVSIVVVAPPGWEAEVHRAVGSFGVDAVVTGGETRQASVRAGLAVVAADVERILCHDA
                     ARPLASPTLFTRALEALEGWDGVVPVLRVIDTVKRVQDGRIEGTEPRDALALAQTPQA
                     FTSAALRDAHERAAADAFEGSDDALLLERAGYRVRAIDGERTNLKITTAEDLRLAEAL
                     AR"
     gene            complement(1989..3080)
                     /locus_tag="VIH70_04945"
     CDS             complement(1989..3080)
                     /locus_tag="VIH70_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8115741.1"
                     /translation="MLAHEDRSWRGRLPVREASERRLLPRGRLVEFVRLIFVTLFAAA
                     GYTVATRVSRSPSETLAYVVLGTSTGFVLGGVLGRQTATAVRSMEEELRKVPAAEVVA
                     GVGGLIAGLAVAALLSVPVFRLPPLAAWTSVAFTYVTIGSVGFRIGRSKHEELFGLIG
                     MKPRASGAPEKIFVVDTSALIDGRILDLVATGFLSGDLLVHTAVLRELQTIADSSDPR
                     RRARGRRGLDAVGELKRAVTVEVHLIEDDGGSDVDAVLVRVARERGASLVTTDHNLSK
                     VAEAVGVPVPRLNDLAVAFRTPIGIGDEIEVRLVKQGREAGQAVGYLEDGTMVVVEAA
                     GERIGSDAPIVVRNVITTATGRMVFANLQ"
BASE COUNT          474 a         1257 c         1161 g          415 t
ORIGIN      
        1 ctagttacaa gacagctgct ctgcccctga gctacgccgg cgcccgagcg agcctagcag
       61 cggcgagccg ccgggcgggc cacgtgctgg cgttcggcgg ccgctcgcat cacgctcctc
      121 cgagcctgcg gggcaatacg aacgcgtaca gggcggccgc gagcgacgcg gacgcgttca
      181 gcgagccgac gcggccccgc atcgggaggc gcacgagcag gtcgcaggcg gcccgggtga
      241 gccggccgag cccctcgccc tccgaaccga gaacgacggc gacgcggccc tcgggcgatt
      301 ccgcctcgta caccgtccgg tcggcgtgct cgtccagccc gacgaccgtg aagccaacgt
      361 ccttcaggcg ttcgagcccg cgggcgatgt tggcgatccg cgcctgcgtg aggtgcaaca
      421 gcgcgcccgc cgacgcccgg atggcggccg gggtgacgtc cgccgcccgg cgggtgcggg
      481 tgacgagcat cctcgcgccg gcggcctccg ccgagcgcgc cgcggcgccg aggttctgcg
      541 ggtcggtgat cccgtccagg acgacgacca gatcgtcggg cccgaacggc cacgccgcga
      601 gctctcgctc gcccatcggc ttcggcgttc cggtgatcgc cgcgacccct cgatggtccg
      661 ccgcgagccc gtcgagctcg gtacggtcga cctcgcgtac ggggattccc gcctcgcggc
      721 aggtctccag cacgtcccgc agacccgccg tcgagcgcgc gtctcggccg acgaggacct
      781 cccgcgccgc gccggcacgc accgcctcga gaacggcgcg ccggccgccc acgaccggcg
      841 tcctcacgac cgcgccagca ccacgagcgc gaggcagccg acgccgtcgc ccgtcagacc
      901 cagtccctcc ggacgcgtcg ccttgaccga gacccgctcg gtcgggaccc ccagagttcc
      961 gcagagcgct cgccgcatcg cgtctcggac cggcgcgatc gagggacgtt cggcgatcac
     1021 cgtgaggtcg gccgagaccg ggtacgcgcc ggccgttcgc accatcccga cgacacggcc
     1081 gagcaggtcg agcccgccga tcccggcgaa cgcggggtcg gtctcgggga ggtgctcgcc
     1141 cacatcgccg agcgcgacgg ccccgagcag ggcgtccgcg agcgcgtgac acacgacgtc
     1201 gccgtcggaa tgcccggcga ggccctccgg cgcatcgagg cggactccgc cgagcatgag
     1261 ggggcgcccg gcctcgaacg cgtggacgtc gaaccccatg cccacgagcg gttcgctcac
     1321 cgggcgagcg cctccgcgag gcgaaggtcc tcggccgtcg tgatcttcag gttcgtgcgc
     1381 tcgccgtcga tcgcccggac gcggtagccg gcgcgctcga gcaggagcgc gtcgtcggac
     1441 ccctcgaacg cgtcggccgc cgctcgctcg tgcgcgtcgc ggagcgccgc cgaggtgaac
     1501 gcctgcggcg tctgggccag ggcgagcgcg tcgcgcggct cggtcccctc gatcctcccg
     1561 tcctgcacgc gtttgaccgt gtcgatcacc cgaagcaccg ggaccacccc gtcccagccc
     1621 tcgagcgcct cgagcgcgcg cgtgaacagc gtgggcgacg cgagcggccg agccgcgtcg
     1681 tgacagagga tgcgctcgac gtcggcggcc acgaccgcga gcccggcccg caccgacgcc
     1741 tgacgggtct cgccgccggt tacgaccgcg tcgacgccga agctgccgac ggcccggtgg
     1801 acctcggcct cccagcccgg cggggccacg acgacgatgg agacggcggc ggccgaagcg
     1861 agttcgacgg ctcgcgcgac gatggtggcg cccccgaacg gcatgagcgc cttggggagc
     1921 gtggtgtcca tccgctcgcc ccgtccggcg gcgagcacga tcgcgacggc tcggtccacc
     1981 ggccgaggtt actggaggtt cgcgaacacc atcctgccgg tcgccgtcgt gatcacgttc
     2041 cgaacgacga tcggggcgtc cgagccgatc cgctcgcccg ccgcctcgac caccaccatc
     2101 gtgccgtctt ccaggtagcc gacggcctga ccggcctcgc gtccctgctt gaccaaccgc
     2161 acctcgatct cgtcgccgat cccgatcggc gttcggaacg ccaccgcgag atcgttcagc
     2221 cgcgggaccg gtacccccac cgcctccgcc accttcgaca ggttgtggtc ggtcgtgacg
     2281 agcgaggcgc cgcgctccct ggcgacccgg acgagcacgg cgtcgacgtc ggatccgccg
     2341 tcgtcctcga tcaggtggac ctcgaccgtc acggcgcgtt tcagctcacc gacggcatcc
     2401 agaccacgtc ggccgcgagc tcgtcgccgg gggtccgagg agtcggcgat ggtctgcagc
     2461 tcacggagta ccgcggtgtg gacgagcaga tccccggaca ggaacccggt cgccacgaga
     2521 tccaagatgc ggccgtcgat cagtgcgctg gtgtccacca cgaagatctt ttccggggcg
     2581 cccgaggctc ggggcttcat gccgatcagg ccgaacagct cctcgtgttt cgaccgaccg
     2641 atccggaagc cgacggagcc gatcgtgacg taggtgaacg ccacgctggt ccatgcggcg
     2701 agcggcggca gccggaacac ggggacggag agcagggccg cgaccgcgag gccggcgatc
     2761 aggcctccga cgcccgcgac gacctcggcc gccgggacct tgcggagctc ctcctccatc
     2821 gaacgcacag cggtggccgt ctgccggccg agcacccctc ccaggacgaa gccggtcgac
     2881 gtcccgagga ccacgtaggc cagggtctcc gacggagacc tggacacgcg ggtggcgacc
     2941 gtgtagccgg ccgcggcgaa cagggtgacg aagatcagcc ggacgaactc gaccaggcga
     3001 cctctgggca acagacggcg ctcggaggcc tcccgaactg ggaggcgtcc gcgccacgag
     3061 cgatcctcgt gcgcgagcat ctcccctcaa gcctcgacgc cgcgggcggt cgacttgaac
     3121 cgggggcgcc tgaaccgggt gtgcccgagc gaagccggtg gtgcctcgat cggtactagt
     3181 gagcccggcc cgaggcctcc gccatgacgc gcgcgccgtg cgactcgtcg agcaccttgt
     3241 cgatcatggc ctcggccttc tcctcgcccc cgccggtggc gtaggtgagc tcggagatca
     3301 ggatctg
//
LOCUS       DATBRK010000163         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_460989, whole genome shotgun sequence.
ACCESSION   DATBRK010000163 DATBRK010000000
VERSION     DATBRK010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460989"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VIH70_04950"
     CDS             <1..447
                     /locus_tag="VIH70_04950"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8115742.1"
                     /translation="IDANRYMALGTADEAGNPWVSPVWFASEDYRNFHWVSSPDAKHS
                     RNLAAHPEVAIAIFDSSVPVGGAQAVYMKGVAKELTGAELEQGLEVFDRVSRQDIGRA
                     FGLDDVQGSALFRLYRATVSEHWVLIPGRDPDRGSGVDRSERLSLD"
     gene            738..1310
                     /locus_tag="VIH70_04955"
     CDS             738..1310
                     /locus_tag="VIH70_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8115743.1"
                     /translation="MRNTSTGNGKVVVNRSMSLDGFIAGPGHAMDWVFDFVAPDEFPD
                     IAAATGAMLIGRRTYEVGKRGIETASRGKISGEDYGGGWSGPEFVLTHDPPDPPDPAV
                     TFLSGDIGEAVATARSAAGGKDLEILGADVAGQCLRRGLVDEILVYVLPVLLGDGIRF
                     SSPGLARIDLEPISSTQSGAVTILRFRVRK"
BASE COUNT          305 a          571 c          566 g          279 t
ORIGIN      
        1 atcgacgcga acaggtacat ggcgctcggc acggctgacg aggcagggaa tccgtgggtc
       61 tcgcccgtgt ggtttgcgtc cgaagactac cggaacttcc actgggtctc atcgcccgac
      121 gcgaagcact cgcggaacct cgccgcccac ccggaggttg cgatcgcgat cttcgactcg
      181 tcggtcccgg tcggcggcgc gcaagccgta tacatgaagg gcgtggccaa ggagcttacg
      241 ggcgccgagc tcgaacaggg tctcgaggtc ttcgaccgcg tgtcccgaca ggacatcggt
      301 cgcgcctttg gactggacga tgtgcaggga tcggcactct tccggctcta tcgcgcgacg
      361 gtctcggagc actgggtgct gatcccgggc cgggatccag accgtggctc gggggtcgac
      421 cgcagcgagc gcctgtcgct cgattaagag attccgcagg taggtgcgcg tcgttgacgt
      481 ccagggtgtt ccgggtgatg gacagttcgt agaacttctg ccagcagcct tcgagctgaa
      541 cacgcttagg tagactccgc tcacgccgac gaaatccggg tcaaggaacg gcctgaccgc
      601 gtcaagccac gcctcgcgat cgtcgccgtc gtctgcggtg ggctttatcg ctggcgaggc
      661 ggcgagattc gggcgcgggt aacgtcatca ctcgtagccc ggggtgacgg cccgcgaagg
      721 tcttctggag gagcacggtg cgaaacacga gcacgggaaa cggcaaggtg gtcgtgaaca
      781 ggtcgatgtc gcttgacggc ttcatcgccg gtcccggcca cgcgatggac tgggtattcg
      841 acttcgtggc gccggatgag ttcccggaca tcgctgcggc caccggcgcc atgctcatcg
      901 gccggcggac ctacgaggtc ggcaagcggg gcatcgagac cgcgagccgc ggcaagataa
      961 gcggcgaaga ctacggcggg ggatggtccg gacccgagtt cgtcctcacc cacgacccgc
     1021 cggacccgcc ggaccccgcg gtcacgttcc tcagcggtga catcggggaa gccgtcgcca
     1081 cggcgcgcag cgccgccggg ggcaaggacc tggagattct cggcgccgac gtggccggcc
     1141 agtgcctgcg gcggggtctc gtcgacgaga tcctggtgta cgtacttccg gtgctgctcg
     1201 gcgacggcat ccgcttctcg tccccgggcc tagccaggat cgacctggaa ccgatcagca
     1261 gcacgcagtc gggcgccgtc accatcctca ggttccgcgt ccgcaagtag ccccggctta
     1321 cccgccgcag cggcaacgat gccgaccgcg acgcccacca cgaaggctgg gcccgaccag
     1381 accgtaaccg caccttcaaa ctggagcact gagactacga cactgaccag attgccggct
     1441 tcgaccacga catcggcgca gttcttgtga aagccacgac cgtcaccggt gaggccgaac
     1501 tcgccgccac ccctgaagca tggagcctcc ggcccgaaca gttcccctac ccctggcaaa
     1561 ccgatgaccc gaagtagcca ccaacctcac cgccccgaac gacccatcca caggtcttga
     1621 caattcctca tgacaggtgc agtcgtcgca atgcgggtca ggagcccggt gggaggttgc
     1681 tacgcgttgt tgctagtgcc gaggttcttc agcgcatcgg c
//
LOCUS       DATBRK010000164         7388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_310347, whole genome shotgun sequence.
ACCESSION   DATBRK010000164 DATBRK010000000
VERSION     DATBRK010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310347"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1830
                     /locus_tag="VIH70_04960"
     CDS             214..1830
                     /locus_tag="VIH70_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8115744.1"
                     /translation="MAAPGATASIERAVEQAGGVVERRLSIISGFSAAVPADALPTLR
                     VLPGVVSVSADSEMQPQDATYDPATDVDSMSAVTRYTGAVDWWNSGYTGAGVDVAVID
                     TGVSPVDGLATPGKVLNGPDLSIESQAPNLRYLDTFGHGTFMAGLIAGHDPELTAPYA
                     DAPATAYRGIAPDARIVSVKVATADGGTDVSQVIAAIDWVVQHAHDPGLNIRVLNLSY
                     GTNSTQLYTIDPLAFAAEQAWKHGIVVVASGGNSGFQRHMNNAPALSNPAFDPLLLAV
                     GASDSQGTDTLADDLVPDFSPWPKRGATRGVDLVAPGTHLQGLRVPNSYIDANHPEGL
                     LGDRYFRGSGTSQAAAIVSGAAALVLQKFPSATPDQVKQLITTTAYPISGKSQAIGGG
                     ELQLSDALTAPLPTFTQLWAPSTGIGSLELARGQDHISMDGVVLDGERDIMDSPFNAA
                     EMASLEAQAKSWSGGDWNAKTWSGEAWSGDAWEAKTWSGDSWSAKTWSSCIFSSNYWE
                     AKTWSAKTWSGDSWSGASWSGGVWATESWS"
     gene            1840..4152
                     /locus_tag="VIH70_04965"
     CDS             1840..4152
                     /locus_tag="VIH70_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8115745.1"
                     /translation="MTSFRELPLSARLVVFGYVALGAFGVLLRVPEMRTWSWGDVAAT
                     FGLAIAAALSEQFTVAMKHRTEVENFSVTDAVWVPALILVPPSVLTLSVLLGSLAGHS
                     YRRWAWYKVAFNASQFVVAITVAEFVYRLFDPPSTFSFMTLVACAVAMSCYLAINEIS
                     VALIISRVEKTPLREVVVLPGSLNLLHAGGNLTIGMLAALVWHAGPMGLPLLIAPVIL
                     SFFAYRGWLENKREDEQRRERDRMRTLYEAGRALVGPLDANFDFQPFLRSVQQLVDAA
                     GTELVVRDEAGLRVYNSESGLYLTVQTEMGPGDPTAYVSTRPGLATYVADVRDEANEI
                     GILAVHRATELSPSECALVDSLASQVSARQRNQQLFDETVEQRGHLADVVGSSSDGIF
                     VVSGAGTVLSWNPAMERITGYLADEVVGRRCGDVLRIPGEPSTDGSSPTELAFGIMEP
                     QDALILRRDGSDRWIRYSSSTMPERHGGGTSQVVTARDVTAELEAEQMKRDFVATVSH
                     ELRTPLTPLKGLLQSLNKGLVEDSPQARREYHAIMLRQADRLETLISDLLDVSRFDAG
                     HLPMDAVPVELGALLEREIADAGQLTAAREVRFERPERPVWVTADPLRLGQIVTNLLS
                     NAFKYSPKGAPVIVRLTKLEDFAQVSVQDRGEGIAIDDQDRVFERFYRVDTRLTQTTG
                     GFGLGLFIARRLAEAMGGGLVLSSRPGEGSTFSVSLPLLMAAPGAEDLGGERRSLRDT
                     DADQHGPDADEQIGQVLTDVVSQVLAQRSG"
     gene            4468..7266
                     /locus_tag="VIH70_04970"
     CDS             4468..7266
                     /locus_tag="VIH70_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8115746.1"
                     /translation="MAGDVIGRESELAILHSFLDSIPTGSSALLLSGDPGIGKTTVWK
                     EGLADAQLRRYRTLSCGPVEAETRLSYAALGDLLEPILEEALPTVPEPQRLALEVALL
                     RTPRSGARADQRAVSLAVLGCLRSVASTSPVVVAVDDVQWMDVPSVRVLQFVVRRLKD
                     EQVGLMTAARGAPADDDPLGVVSALTEDHVHAVHVGPLSLDALERVLRAKVGEGFSRT
                     TLLNLHEMSGGNPFFAQEIGLALLRRGGDVVAAGEGLPIPDRLQVLIEDRLQGLPAET
                     VEALEVVSALSTPTLDAIGAATDPSEVDRRLGPAIENGVVEVVGDRLRFTHPLLASAV
                     YQRIPPARRRELHARLATIVRDPEERARHLALSVVGPDVAVAAALEEAAALAFSRGAP
                     QSAAELWEMARRATPPDRREDQVRRTHQAGVAHHECGDTSLARSVLEQAVDLSKAGPA
                     RARVLLDLGMALAENEGWRGAWAVFEAARGEAGDDLGLRARIEQNLGYAWLFRGDLAA
                     SERHARAALQLAEELQEPRVMAEAFTAYPFVEFLLGRGVDQQLLDRGISLEDHMEGEF
                     KSHVLRASFVMAQLLKFTDRLDEARRTFTELLGDAAAHGVEGPIPQIRYHLAELECRA
                     GNWDAAMEHARESRAAAQRIRMGAMSSEGHFAVGLVEAHLGRAESARLAALEGLRVAE
                     AAGEILLLIPNLAVLGFLELSLGRPAEADAFLFRAIELEQAMGVREPAYFRMVPDEVE
                     ALVALGRLDQADALLAPFEDAGRSLDRAWAAATGARCRALVLAARGDLQGASVAADEA
                     VRHHDRLPLPFELGRTHLVRGAVERRAKRKRGARDTLTKALEIFDGLGAALWADKTRA
                     ELARIGGRAASSLDLTPTEARVAALVAAGSTNREVADALFVSVHTVEANLKRIYRKLG
                     IRSRTELASALPSRPSGS"
BASE COUNT         1137 a         2489 c         2561 g         1201 t
ORIGIN      
        1 ggacgccggg cgaccgatgg atgggaacac ccgggcgagg tcgaggaagg atgcgacgtg
       61 aggacccggg gcggcacagg acggaggcgg agaaccgccg cctggggtcg agcgcgcacg
      121 atcgcaccgg tcctgtctct cgcgatcgcg gtcggcgcgc cgagcgccgt ggccgcctcc
      181 ccgggttcgg ccccgcgcgc acatgtcatc gtcatggcgg cgccgggtgc gaccgcctcg
      241 atcgagcgcg ccgtcgagca ggccggcggc gtggtcgagc ggcgcctgtc gatcatcagc
      301 gggttctcgg cggccgttcc cgccgacgcc ctcccgaccc ttcgcgttct tcccggagtc
      361 gtctcggtca gcgcggactc cgagatgcag ccccaggacg ccacgtacga tcctgcgacc
      421 gacgtcgact cgatgagcgc cgtgacccgg tacacgggcg cggtcgactg gtggaactcc
      481 ggctacacgg gagccggcgt cgacgtggcg gtgatcgaca ccggggtgtc cccggtcgac
      541 ggcctcgcga cgcccggcaa ggttctcaac gggccggacc tctcgatcga gtcccaggcg
      601 ccgaacctca ggtacctcga cacgttcggc cacgggacgt tcatggccgg actcatcgcc
      661 gggcacgatc cggagctcac cgctccgtac gcggacgctc cggccacggc ctaccggggc
      721 atcgcccccg atgcccggat cgtcagcgtc aaggtcgcga cggcagacgg cgggaccgat
      781 gtctctcagg tgatcgccgc catcgattgg gtcgtccagc acgcacacga ccccggtctg
      841 aacatccggg tcctgaacct ctcctacggg accaacagca cgcagctgta cacgatcgat
      901 ccactcgcct tcgcggccga gcaagcgtgg aagcacggca tcgtcgtcgt cgcgtcgggt
      961 ggcaacagcg gcttccagcg ccacatgaac aacgcgccgg cgctgtccaa ccccgcgttc
     1021 gatccactcc tgctcgccgt cggtgcgtct gactcgcagg gaacggacac gctcgccgac
     1081 gatctcgtgc cggatttctc accgtggccc aagcgcggag cgacgagggg ggtcgacctc
     1141 gtcgcacccg gtacccatct ccaggggctg cgcgtgccga actcgtacat cgacgcgaac
     1201 catcccgaag ggctcctcgg cgaccggtac ttccggggga gcgggacgtc gcaagcggcg
     1261 gcgatcgtgt cgggggcggc ggcgctcgtg ctccagaaat tcccgtccgc gaccccggac
     1321 caggtgaagc agttgatcac caccaccgcg tatccgatca gcgggaagtc ccaggccatc
     1381 ggcggcgggg agctccagct ctcggacgca ctgaccgcgc cgcttccgac gttcacgcag
     1441 ctctgggccc cctcgacggg gatcggctcg ctggagctcg cccgcggcca ggatcacatc
     1501 tcgatggacg gcgtggtgct ggatggtgag cgggacatca tggattcgcc tttcaacgct
     1561 gccgagatgg cctcgctcga ggcgcaggcg aagtcctggt cgggcggcga ttggaacgcc
     1621 aagacctgga gcggggaggc ttggagcggg gacgcgtggg aggccaagac ctggagcggg
     1681 gattcctgga gcgccaagac ctggtcctcc tgcatcttct cgagcaacta ctgggaggcc
     1741 aagacctgga gcgccaagac ctggagcggc gactcctgga gtggcgcctc gtggagcggt
     1801 ggcgtgtggg ccacggaatc ctggagctga tcgagtgaga tgacctcctt ccgcgagctg
     1861 ccgctctcgg cccgactcgt cgtcttcggc tacgtcgcgc tgggcgcgtt cggcgtcctg
     1921 ttgcgggtcc cggagatgcg gacctggtcc tggggggacg tcgccgcgac cttcgggctg
     1981 gcgatcgcgg cggctctcag cgagcagttc acggtcgcga tgaagcaccg cacggaggtc
     2041 gagaacttct cggtcacgga cgcggtatgg gttccggcgc tcatcctcgt gccgccgagc
     2101 gtcctcacgc tcagcgtgct cctggggagc ctggcgggac actcctaccg gcgctgggcc
     2161 tggtacaagg tcgccttcaa cgcgagccag ttcgtcgtcg ccatcaccgt ggcagagttc
     2221 gtctaccggc tgttcgatcc tccctcgacg ttcagcttca tgaccttggt ggcgtgcgcc
     2281 gtcgccatgt cgtgctatct cgccatcaat gagatctcgg tcgcgctgat catctcgcgt
     2341 gtagagaaga cccctctccg cgaggtcgtg gtcctcccgg ggagtctgaa cctcctccat
     2401 gccggcggga acctcacgat cggcatgctc gccgcgctcg tgtggcacgc ggggcctatg
     2461 ggcctccccc tgctgatcgc acccgtcatc ctgtccttct tcgcctaccg aggatggctg
     2521 gagaacaagc gggaagatga gcagcgacgg gaacgtgacc gcatgcggac cctctacgag
     2581 gccggaaggg ccctcgtcgg tccgctcgac gcgaacttcg acttccaacc gttcctgcgg
     2641 tccgtccagc aactcgtcga cgcggcgggg accgaactgg tcgtgcggga cgaagcgggg
     2701 ctgcgcgtct acaactccga gagcggtttg tacctgaccg tccagaccga gatgggcccc
     2761 ggagacccga cggcgtacgt gagcacgcgc ccggggctcg ccacgtacgt cgccgacgtc
     2821 cgcgacgagg cgaacgagat cgggatcctc gccgtccacc gcgccaccga gctgtctccg
     2881 tcggaatgcg cgctggtcga ctcgcttgcc tcccaggtct ccgcgaggca acggaaccag
     2941 cagctcttcg acgagacggt cgagcaacgt ggccacctgg cggacgtggt cggcagcagc
     3001 tcggacggca tcttcgtcgt ctccggagcg ggcacggtgc tctcgtggaa cccggcgatg
     3061 gagcggatca ccggctacct cgcggacgag gtggtcggtc gtcgttgcgg cgacgtgctc
     3121 cggatccccg gcgagccgag cacggatgga tcgtccccga cggagctcgc gttcgggatc
     3181 atggagcctc aggacgcgct gatcctgcgg cgcgacggat cggatcggtg gatccgctac
     3241 tcgagcagca cgatgcccga gcgccacgga ggcggaacga gtcaggtcgt gaccgctcgc
     3301 gacgtgacgg ccgagctcga ggccgaacag atgaaacgcg acttcgtcgc caccgtgtcc
     3361 cacgagctca ggaccccgct cacacccctc aagggcttgc tccagtcgct gaacaagggg
     3421 ctggtcgagg actctcctca ggcacgccgc gagtaccacg cgatcatgct gcgccaggcc
     3481 gaccggctgg agacgctcat ctccgacctc ctcgatgtct cacgcttcga cgccgggcac
     3541 ctgccgatgg acgcggttcc ggtggagctc ggtgcgctcc tggagcgcga gatcgcggac
     3601 gcgggccagt tgacagcagc gcgcgaggtt cggttcgagc gtccggaacg ccccgtctgg
     3661 gtaacggccg acccgctccg cctcggccag atcgtcacca acctgctctc gaacgcgttc
     3721 aagtactcac cgaagggagc cccggtgatc gtgaggctca ccaagctcga ggatttcgcc
     3781 caggtctcgg tgcaggatcg gggcgaaggg atcgcgatcg acgaccagga tcgcgtgttc
     3841 gagcgcttct accgcgtgga cacgagattg acgcagacca caggcggttt cggcctgggc
     3901 ctgttcatcg cccgccgtct ggccgaagcg atgggtgggg gtctggttct gagctcgcgg
     3961 ccgggggagg gatccacgtt ctcggtcagc ctgccgctcc tcatggccgc gccgggggcg
     4021 gaggatctgg ggggcgaacg ccgttcattg cgtgacaccg acgccgatca acatggacca
     4081 gatgccgatg agcagatcgg acaggtactc accgacgtcg tttcccaggt actggcgcaa
     4141 cgctcgggat agcgctgcct gttcctctcg cggctcgggt tcgagaggcg atccgtgtac
     4201 gcgggagtgc ggacacctgt acagccatcg gaagagacca cgtatctgca cgaggccggc
     4261 cagcttcaat cagagcggag tcgagtatcg gacccaccga gaagttgacc gagccacgca
     4321 aagttcacca ataaaagttc accaatagaa gtctgacccg gtacacattt ggtactaggc
     4381 cggtacatgg ctctgggcct cgacgggtct gcaaggttcg gacccgccat ccttagaatc
     4441 ctggcgtcca ccgacgcggg agcgcctttg gccggtgacg tcatcgggcg ggagtccgag
     4501 ctcgcgatcc tccatagctt cctggactcg atcccgaccg ggtcgagcgc gttgcttctg
     4561 tcgggtgatc ctggcatcgg gaagaccacc gtgtggaagg aggggctggc ggatgctcag
     4621 ctccggcgct accgcaccct ctcatgcggt ccggtcgagg ccgagacacg cctgtcctac
     4681 gctgcactcg gtgacctcct cgagcccatc ctggaggagg cactccccac cgtaccggag
     4741 ccacagcgcc tggccctcga ggtggccctc ttgcgcaccc cgagatcggg cgcccgagcc
     4801 gaccagcggg ccgtttccct ggcggtgctt ggttgcctcc gatcggtggc ctccacctct
     4861 ccggtcgtgg ttgccgtcga tgacgtccag tggatggacg tcccttcggt tcgggtgctc
     4921 cagttcgtcg tgcggcgcct gaaggacgag caggttggtt tgatgaccgc cgctcgaggc
     4981 gcacctgccg atgacgatcc cttgggcgtc gtgtcggcct taaccgagga tcacgtgcac
     5041 gccgtccacg tcggaccgct gtcgcttgac gccctcgaac gggtccttcg agccaaggtg
     5101 ggggaagggt tctcccggac cacactcttg aacctccacg agatgtcggg aggcaatccc
     5161 ttcttcgccc aggagatcgg actggcgctc cttcgccgcg gaggcgacgt ggtggcggcc
     5221 ggcgaagggc tcccgatccc ggaccgcctc caggtactca tcgaggaccg cctccagggg
     5281 cttcccgcgg agaccgtcga agcgctcgag gtcgtctcgg ccttgtcgac accgacgctg
     5341 gatgcgatcg gtgccgcaac cgatccctcc gaggtcgacc gtcgccttgg tccggccatc
     5401 gagaacgggg tcgtcgaggt cgtcggcgac cggcttcggt tcacccaccc cctccttgcg
     5461 tccgccgtct atcagaggat cccgccggct cggcgtcgcg agctgcacgc gcggctcgcg
     5521 acgatcgtcc gcgatccgga ggaacgagcc cgccacctgg ccctgtccgt cgtgggcccg
     5581 gacgtcgccg tcgccgccgc cctggaggag gccgccgcat tggccttctc gcggggagcg
     5641 ccgcaatctg cggccgagct ctgggagatg gcccgccgcg ccacgccgcc ggaccgacgt
     5701 gaggatcagg tgcgtcggac ccatcaggcg ggcgtggctc accacgagtg cggggacacc
     5761 tcgctggcgc ggagcgtcct ggagcaagcg gtggacctct cgaaggccgg gccggctcgg
     5821 gcccgcgtgc ttctggacct cgggatggcc ctcgccgaga acgaagggtg gcggggggcc
     5881 tgggccgtgt tcgaggctgc tcggggcgag gcgggggacg atctcgggct cagggcacgg
     5941 atcgagcaga atctcgggta cgcctggctg ttccggggag acctcgcggc atccgaacgg
     6001 cacgctcgcg cagcactgca gttggccgag gagctgcagg aaccacgggt gatggccgag
     6061 gcgttcacgg cgtatccatt cgtcgagttc ctcctcggac ggggggtcga tcagcagctg
     6121 ctcgatcgcg ggatctcctt ggaagaccac atggagggcg aattcaagtc ccacgtcctg
     6181 cgtgcaagct tcgtcatggc tcagctcctc aagttcaccg atcgcctgga cgaggcccgc
     6241 cggacgttca ccgagctcct gggtgatgcc gcggcgcacg gcgttgaggg cccgatcccg
     6301 cagatccggt accacctcgc cgagctggag tgccgggcgg gcaactggga cgcagctatg
     6361 gagcacgccc gggagagcag ggcggccgcc cagcggatcc gcatgggggc catgtcgtcg
     6421 gagggccact tcgcggtggg cctcgtggag gcccatctgg ggcgggccga gtcggcgagg
     6481 ctggcagccc tcgaggggct tcgggtcgcc gaggcggcgg gcgagatcct cctcttgatc
     6541 ccgaacctgg cagtcctggg gttcctcgag ctttccctgg gccgacccgc cgaggccgat
     6601 gcgttcctgt ttagggccat cgaactcgag caagcgatgg gcgttcggga gcccgcctac
     6661 ttccggatgg tcccggatga ggtcgaggcg ctcgtcgccc tcgggcggct cgaccaggcc
     6721 gatgcgctcc tcgcaccctt cgaggacgcc ggcaggagcc tcgaccgggc atgggccgcg
     6781 gcgacgggag ccaggtgccg ggcgctcgtg ttagcggccc ggggtgatct ccagggtgca
     6841 tcggtcgcag ccgatgaggc cgtccgtcac cacgatcgcc taccccttcc cttcgagctg
     6901 ggtcggacgc acctggttcg cggagcggtg gagcgtcggg cgaagaggaa gcggggggct
     6961 cgggacacgc tcacgaaggc cctggagatc ttcgacgggc tgggggccgc gttgtgggcg
     7021 gacaagacac gtgccgagct ggcgcgaatc ggcggccgcg ctgcctcgtc gctggacctg
     7081 accccgaccg aggccagggt ggcggcgctg gtcgccgccg ggagcaccaa ccgagaggtt
     7141 gccgatgcgc tgttcgtcag cgtccacaca gtggaggcca atctcaagcg gatctaccgc
     7201 aagctgggca tccgctcccg aaccgaactg gcctcggcgt tgccatcccg gccctccggg
     7261 tcgtgagcaa gacctaccgg tcacggtctc gatcggtacg gatttcgggg attcctcgcg
     7321 tcggtgctct ctctagcctc gggatcagag atcggtaccc gagtgaaggg agggatcatg
     7381 agcacgaa
//
LOCUS       DATBRK010000165         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2338395, whole genome shotgun sequence.
ACCESSION   DATBRK010000165 DATBRK010000000
VERSION     DATBRK010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338395"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..885)
                     /locus_tag="VIH70_04975"
     CDS             complement(82..885)
                     /locus_tag="VIH70_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8115747.1"
                     /translation="MVTIAHVSDPHVGSPYFIPNLMNRVIVELNELEPDVVICSGDLT
                     NEGYRQEYKNWVAYAERIRAPMYTVPGNHDARNVGYLHFEELVGPRHWSADVKGVRLV
                     GVDSSEPDINEGQVGRERYTWIHERFEVPADLKVFVLHHHLLPIPGTGRERSTVMDAG
                     DLLEELIQSGVNIVLSGHKHVPYVWRLEDLYVANAGTVASLRLRGYTKPCYNVLEFEG
                     DEVKITRKYPFGGGSVIAHFQLSTGEQYHRELEPPVQQRRLPAAHQGES"
     gene            989..2155
                     /locus_tag="VIH70_04980"
     CDS             989..2155
                     /locus_tag="VIH70_04980"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEY8115748.1"
                     /translation="MNALDLLLIVLLALSGVSGYRRGFTLQAFGFGGLLIGLVVGALL
                     APFFANIVDGPNARAGLAVLVLLAVAALGNAAGWFIGVHARHRAASKFAKADSVAGSA
                     VAVVASLLAIWFLALNLANGPFPAIATEIRGSAVVRTLDAALPQPPSLLAQVRRLFNR
                     FAFPDVFSGIPPLPAEPVRPPSQAEARAAAEAAAASTLQVIGPACDVIQEGSGFVAAE
                     GYVVTNAHVVAGMDDPEVRSAAGRSDATTVLFDPDLDLAILSVPGATGPPLPLATEPL
                     DRGAPGAVLGYPQGGPLDVRRAAVRRTIDAVGRDIYGHGEVERLVLELQTLVRPGNSG
                     GPFVLPDGRVAGVVFAASSGDDDVGYAIAADEVQDPLRTATGQTTPVDTGPCIG"
     gene            complement(2199..>3386)
                     /locus_tag="VIH70_04985"
     CDS             complement(2199..>3386)
                     /locus_tag="VIH70_04985"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY8115749.1"
                     /translation="ARQGTEENDWRDEQPGKILHEVRDGELARAGIMPHSPYYGSVDS
                     TPWFLIVYAQHLRWTGDVEFARKLLPSAEAALAWIDRYGDLDGDGFVEYLSRSPRGIR
                     NQGWKDSHDSMVHADGRLAEPPIALSEVQGYVYLAKTRMADVYRALGRPEDARRLEDE
                     AERLKVRFNEAFWMEDERYFAAALDARKQPLRTLMSNPGHCLYAGIVDEDKALPLAKR
                     LLAPDMFSGWGIRTMSRSAASYNPMSYHNGSVWPHDNALIAAGLKRYRFARATNRVAT
                     ALFDAAVSADYLRLPELFCGFTRRSPNRPVSYPIACSPQAWAAGSPYLMLQAMLGLSA
                     RANENLLTINLPHLPTWLNTVEVRNLSVGESTVSMVFRREGEITSFSLLSREGDVRVV
                     MEE"
BASE COUNT          445 a         1199 c         1188 g          554 t
ORIGIN      
        1 gtgacgggcg ggtagtgctc accgtcgacg aggacgacga ccttcatccg atgggctcct
       61 tcggggcggt gcggttcgga gctaggactc cccctggtga gccgcgggga ggcggcgctg
      121 ctgcaccggc ggctcgagct cgcgatggta ctgctccccg gtcgacagct ggaagtgggc
      181 gatcacgctg cccccgccga acgggtactt ccgcgtgatc ttcacctcgt cgccctcgaa
      241 ctccaggacg ttgtagcagg gcttcgtata cccgcggagc cggagcgacg cgaccgtgcc
      301 ggcgttcgcc acgtagaggt cctccaggcg ccacacgtat gggacgtgct tgtggccgga
      361 gagcacgatg ttgacccccg actggatcaa ctcctccagg aggtcgccgg cgtccatcac
      421 ggtgctccgc tctcgcccgg tgcccgggat cggcagcagg tggtggtgca ggacgaagac
      481 cttcaggtcg gcggggacct cgaaccgctc gtggatccag gtgtaccgct cgcgcccgac
      541 ctgaccctcg ttgatgtcgg gctcgctgga gtccacgccg acgaggcgga cacccttcac
      601 gtccgcgctc cagtgtctcg ggcccacgag ctcctcgaag tgcaggtacc cgacgttgcg
      661 ggcatcgtgg ttgccgggga ccgtgtacat cggcgcgcgg atccgttcgg cgtaggcgac
      721 ccagttcttg tactcctgcc ggtacccctc gttggtgagg tcgccgctgc agatgacgac
      781 gtcgggctcg agctcgttca gctcgacgat caccctgttc atcaggttcg gaatgaagta
      841 gggggagccg acgtgcggat cggagacgtg cgcgatcgtt accacgttgc ctcagtctag
      901 cttcgtctct gctcgttccc ggcctcgtcc tctgggctcg cccctggtcg accggggacc
      961 gcgtcggccc gtgggacgat acggcggcat gaacgccctc gacctgctcc tgatcgtcct
     1021 cctggcactc tccggggtct ccggctaccg gcggggtttc acactgcagg ccttcgggtt
     1081 cggcgggttg ctcatcggcc tggtcgtggg cgctttgctc gccccgttct tcgccaacat
     1141 cgtcgacgga ccgaacgccc gagccggcct cgccgtcctg gtcctgctcg cggtggccgc
     1201 gctcggcaac gcggccgggt ggttcatcgg cgtccacgca cgccaccgcg ccgcgtcgaa
     1261 gttcgcgaag gcggactcgg tcgcgggctc ggccgtggcg gtcgtggcgt ccctgctggc
     1321 gatctggttc ctcgcgctga acctcgcgaa cggcccgttc ccggccatcg cgaccgagat
     1381 ccgggggtcg gcggtcgtgc ggacgctcga cgccgccctg ccgcaaccgc cgtcgctgct
     1441 cgcccaggtc cgtcgcctgt tcaaccggtt cgcgttcccc gacgtgttct caggcatccc
     1501 gcccctgccg gccgagcccg tccggccacc gagccaggcg gaggctcggg ccgcggcgga
     1561 ggcggccgcg gcgagcaccc tccaggtgat cggaccggcg tgcgacgtga tccaggaggg
     1621 ttcgggcttc gtcgccgccg agggctacgt cgtgacgaac gcgcacgtcg tggccggcat
     1681 ggacgatccc gaggtgcgtt cggcggcggg ccggtcggac gcgaccacgg tgctgttcga
     1741 ccccgatctg gatctggcga tcctgtcggt gccgggcgca accggacccc cgctcccgct
     1801 cgccaccgag ccgctggacc gcggcgcgcc gggggcggtc ctcgggtacc cgcagggtgg
     1861 cccgctcgac gtccgccgtg cggccgtgcg gcggacgatc gacgcggtcg ggagggacat
     1921 ctacgggcac ggcgaggtcg agcgtctggt gctcgagttg cagacgctgg tccgcccggg
     1981 caactccgga gggccgttcg tcctgcccga cgggcgggtc gccggggtcg tgttcgccgc
     2041 gtcgtccggc gacgacgacg tcggctacgc gatcgcggcc gacgaggtcc aggaccctct
     2101 gcggaccgcg acggggcaga ccacgccggt cgacaccggg ccctgcatcg gctgaccgct
     2161 cggccgacga ccaggccgcg ccgcggttcg ccgccctcct actcctccat cacgacgcgc
     2221 acgtcgccct cgcgcgacag gagggagaag gaggtgatct cgccctcgcg acggaacacc
     2281 atgctcacgg tgctctcgcc gaccgacagg ttgcgcacct cgacggtgtt cagccacgtc
     2341 ggcaggtgcg gcagattgat cgtgagcagg ttctcgttgg cccgcgccga caggccgagc
     2401 atcgcctgca gcatcaggta cggcgagccg gccgcccacg cctgcggcga gcaggcgatc
     2461 gggtagctta cggggcggtt cggggagcgc cgcgtgaacc cgcagaacaa ctcgggcagt
     2521 cggaggtagt cggcggagac cgccgcgtcg aacagcgcgg tcgcgacgcg gttggtcgcc
     2581 cgggcgaacc ggtaccgctt cagccccgcc gcgatcagcg cgttgtcgtg aggccacacc
     2641 gatccgttgt ggtagctcat cgggttgtac gaggccgccg acctgctcat cgttcggatg
     2701 ccccagcccg agaacatgtc cggagcgagc agccgcttgg cgagcggaag ggccttgtcc
     2761 tcgtccacga tcccggcgta caggcagtgg cccggattgg acatcagcgt tcggagcggc
     2821 tgcttgcgtg cgtcgagcgc ggccgcgaag taccgctcgt cctccatcca gaacgcctcg
     2881 ttgaagcgga ccttcaggcg ttcggcctcg tcctccagtc ggcgcgcgtc ctccggccga
     2941 ccgagcgcgc ggtacacgtc ggccatccgc gtcttcgcga ggtagacgta cccctgcacc
     3001 tccgagagcg cgatcggtgg ttcggcgagc cggccgtcgg cgtggaccat cgagtcgtgc
     3061 gagtccttcc atccctggtt gcggatcccg cggggcgagc gggacaggta ctcgacgaac
     3121 ccgtcgccgt ccaggtcgcc gtaccgatcg atccacgcga gcgcggcctc cgccgacggg
     3181 aggagcttcc gagcgaactc gacgtcgccc gtccagcgca ggtgctgcgc gtacacgatc
     3241 aggaaccacg gcgtcgagtc gacggacccg tagtacggcg agtgcggcat gatccccgcg
     3301 cgcgcgagct cgccgtcgcg gacctcgtgc aggatcttgc ctggctgctc gtcacgccag
     3361 tcgttctcct ccgtgccctg acgcgc
//
LOCUS       DATBRK010000166         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1182687, whole genome shotgun sequence.
ACCESSION   DATBRK010000166 DATBRK010000000
VERSION     DATBRK010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182687"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VIH70_04990"
     CDS             <1..811
                     /locus_tag="VIH70_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8115750.1"
                     /translation="TTVERDGEELVAPDGGRIALGSAEHLAPTEPTKIVCVHLNYESR
                     VKEFLAQMPTAPTYFHKPVSALNAHGGEVVRPPRCRYLNYEGEIAIVIGRTCRHIAPD
                     EAADHIAGYTIANDYGLHDFRETDAGSMLRVKGSDTLCPLGPGLVDDWDFRGKRIRTL
                     VNGDVRQDGSTDEMMWDMHYLVADIARTITLVPGDVILSGTPANSRPVEPGDVVEVEV
                     EGLGTLRNRIVEGAVPIRVDVGAQPSASEEVVSTAFGGDWEFRGVRAPKRG"
     gene            811..1239
                     /locus_tag="VIH70_04995"
     CDS             811..1239
                     /locus_tag="VIH70_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115751.1"
                     /translation="MDLDALLRAHAFMRVERPPLLWSPDTEDEPFLRMLGEMIALGPG
                     RGNELEDLTLGVSNVVVEPDEDDEEVAWIPDGEYVAITVRGGGAWDDDTWRAGQGPTT
                     GLLCNVGPAADAAGAVYANTRDLVPEGSVTALLPRRVPPT"
     gene            complement(1202..1747)
                     /locus_tag="VIH70_05000"
     CDS             complement(1202..1747)
                     /locus_tag="VIH70_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8115752.1"
                     /translation="MEADFRLETERLRLRRYRPGDLDDLHAMFSDPEHMRWYPEPFPI
                     EGTRAWMDRTFDRYRDDGFGLWVVEERSTGSFLGTAGPTIQEVDGVKHVEIGWHTRPG
                     RKGEGIAPEAGAAARDWAFANLDVDHVISLVRPENQASCRVAEKIGMHVDRQADYKGL
                     LHRIYRIDRITWEERVGAGAP"
     gene            1832..>2736
                     /locus_tag="VIH70_05005"
     CDS             1832..>2736
                     /locus_tag="VIH70_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY8115753.1"
                     /translation="MDIKGKRLRLLYSDVLGVERGKYLFGDVADAGHSAFCIGVFPLT
                     TDKEILDVSRQQFDVGLPDVEAFIDRDTLRPGWEEDTIVGIADVQQHGKALEVDPREV
                     LRRAVEPWRAMGLEPMFAFESEFYLLEPGAEGAWRPVELPSHRVYGTGASVDPTGTID
                     AMVRAAQQCGFPIEGWASEFDTAAYEVNIRYKEVIPAADECFLFRLLVKEIAIKQGKL
                     ATFMGRPFNDRGGSGLHLNISFRREDGSNALHDPKARDGLAPMTRECIAGLLAHHEGI
                     SAIAAPHTNAYKRLQPDMLNGYWAN"
BASE COUNT          424 a          915 c          997 g          400 t
ORIGIN      
        1 gaccaccgtc gagcgcgacg gcgaggagct cgtcgccccc gacggcggcc ggatcgcgct
       61 cgggagcgcc gaacacctgg cgcccaccga gcccaccaag atcgtctgcg tgcacctgaa
      121 ctacgagagc cgcgtgaagg agttcctcgc gcagatgccg acggcgccca cgtacttcca
      181 caagcccgtc agcgcgctga acgcgcacgg cggcgaggtg gtgcgcccgc cgcggtgccg
      241 ctacctgaac tacgagggcg agatcgcgat cgtgatcggc cgcacttgca ggcacatcgc
      301 gccggacgag gcggccgacc acatcgccgg ctacacgatc gcgaacgact acggcctgca
      361 cgacttccgc gagacggacg cggggtcgat gctgcgggtg aagggctcgg acaccctgtg
      421 cccgctcggc ccgggtctgg tggacgactg ggacttccgg gggaaacgga tccggacgct
      481 cgtgaacggc gacgtgcgcc aggacggctc gaccgacgag atgatgtggg acatgcacta
      541 cctggtcgcc gacatcgcgc ggacgatcac gctcgtgccg ggcgacgtga tcctgtcggg
      601 gacgccggcg aactcgcgcc ccgtcgagcc gggcgacgtc gtcgaggtcg aggtcgaggg
      661 tctcgggacc cttcgcaacc ggatcgtcga gggcgcagtc ccgatccgcg tggacgtcgg
      721 cgcgcagccc agcgcgtccg aggaggtcgt ctcgaccgcg ttcggtggcg actgggaatt
      781 ccgcggggtc cgagcgccga agaggggctg atggatctcg atgccctgct ccgggcgcac
      841 gcgttcatga gggtcgagcg accgccgctc ctgtggagcc ccgacaccga ggacgagccg
      901 ttcctgcgga tgctggggga gatgatcgcg ctcgggcccg ggcgggggaa cgagctcgag
      961 gacctgaccc tgggcgtgtc caacgtcgtg gtggaacccg acgaggacga cgaggaggtg
     1021 gcgtggatcc ccgacggtga gtacgtcgcg atcaccgtgc ggggcggcgg cgcgtgggac
     1081 gacgacacgt ggcgcgcggg ccagggaccg accacgggac tgctgtgcaa cgtcggcccg
     1141 gccgcggatg ccgcaggggc cgtctacgcg aacacgcgcg acctggtccc cgagggctcg
     1201 gtcacggcgc tcctgccccg acgcgttcct cccacgtgat gcgatcgatc cggtagatgc
     1261 ggtggagcag gcccttgtag tcggcctggc ggtcgacgtg catgccgatc ttctccgcca
     1321 cccgacacga cgcctggttc tcagggcgga ccagcgagat cacgtgatcg acgtcgaggt
     1381 tcgcgaacgc ccagtcgcgt gctgcggccc ccgcctcggg cgcgatgccc tcgcccttgc
     1441 ggccgggacg ggtgtgccag ccgatctcga cgtgtttcac accatcgacc tcctggatcg
     1501 tcggacctgc ggtaccgagg aacgagccgg tgctgcgctc ctccacgacc cacagtccga
     1561 agccgtcgtc gcgataccgg tcgaacgtcc ggtccatcca ggctcgtgtc ccttcgatgg
     1621 ggaacggctc ggggtaccaa cgcatgtgct cggggtcgga gaacatcgcg tgcaggtcgt
     1681 cgaggtcccc gggccggtag cgccgcaggc gcaggcgttc ggtctcgagg cggaagtcgg
     1741 cctccatgcc gggaccgtag cggagcggct tcgtgcgtgc ctggtctatc ctcggcaggg
     1801 cccatcgcga ggagcatggc cagggggatc gatggacatc aaggggaagc ggctccgact
     1861 cctgtactcg gacgtgctcg gggtcgagcg gggcaagtac ctgttcggcg acgtcgccga
     1921 cgccggccac tcggcgttct gcatcggcgt gttcccgctc acgaccgaca aggagatcct
     1981 cgacgtctcg cggcagcagt tcgacgttgg gcttcccgac gtggaggcgt tcatcgatcg
     2041 ggacaccctt cggccggggt gggaggagga cacgatcgtc ggcatcgccg acgtccagca
     2101 gcacggcaag gccctggagg tcgatccgcg cgaggtgctg cgccgggccg tcgagccgtg
     2161 gcgcgcgatg gggctcgagc ccatgttcgc gttcgagtcg gagttctacc tgctcgagcc
     2221 cggcgcggag ggcgcgtggc gacccgtcga gctgccgtcg caccgcgtct acggcaccgg
     2281 cgcgtcggtc gacccgacgg gcacgatcga cgcgatggtc cgggcggccc agcagtgcgg
     2341 gttcccgatc gaggggtggg cgagcgagtt cgataccgcc gcgtacgagg tcaacatccg
     2401 gtacaaggag gtgatccccg cggccgacga gtgcttcctg ttccgtctgt tggtcaagga
     2461 gatcgcgatc aagcagggca agctcgccac gttcatgggc cggccgttca acgatcgcgg
     2521 cggcagcggg ctgcacctga acatctcgtt ccggcgtgag gacggctcga acgcactcca
     2581 cgacccgaag gcgcgcgacg gcctcgcgcc gatgacccgg gagtgcatcg cgggtctgct
     2641 cgcgcatcac gagggcatct cggcgatcgc ggctccgcac acgaacgcct acaagcgcct
     2701 gcagcccgac atgctgaacg gctactgggc gaactg
//
LOCUS       DATBRK010000167         3988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_27066, whole genome shotgun sequence.
ACCESSION   DATBRK010000167 DATBRK010000000
VERSION     DATBRK010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27066"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VIH70_05010"
     CDS             <1..152
                     /locus_tag="VIH70_05010"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEY8115754.1"
                     /translation="EAGQTTAEYALVILAAAAIAVVLIAWARSSGKLPAFFDSIIDQI
                     AGSAG"
     gene            157..531
                     /locus_tag="VIH70_05015"
     CDS             157..531
                     /locus_tag="VIH70_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY8115755.1"
                     /translation="MRERGSAAVEFALLLPILLLLLLALVQVGVIARDSLVLTQASRA
                     GAREAAVQGTMEAVDEAVRAAAVGLDPDRISVVATWSGARGAPVTVDVAYEAPVASLL
                     AGWLLPASVSLRASATMRQEFA"
     gene            528..959
                     /locus_tag="VIH70_05020"
     CDS             528..959
                     /locus_tag="VIH70_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEY8115756.1"
                     /translation="MTADRPRHGAVVARASPTPRLTSDRGSITLLSAVVLAALVVLAM
                     GASDVARVLHAASRAQTAADAAALAAAQALAIDEEGPEPAALAGEYAERNGAVLETCS
                     CERGTFEATVSVRLPVGDLFLVAGDRTVQARARAQVDLPAP"
     gene            1033..1431
                     /locus_tag="VIH70_05025"
     CDS             1033..1431
                     /locus_tag="VIH70_05025"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HEY8115757.1"
                     /translation="MAMRQRLPVRRGRRSLLATVDYSLAKRALINQVRTGLASWTDIC
                     DAHPELMRAAQHVGEPTRRECPVCHDAKLKLVTYVYGDGLKHDNGRVWPLDVGLDMAA
                     DRPGSWCYVVEVCTECHWNHLHEAFVARAG"
     gene            1514..3784
                     /locus_tag="VIH70_05030"
     CDS             1514..3784
                     /locus_tag="VIH70_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8115758.1"
                     /translation="MTTLDDELAVETAPPRAPRGRRHAPTKKRKGFKAWLRRWWWVFV
                     VMPLVAAIGLLLTLFYIYSQLDLPKTPPPLQTTYIYDRDGHQIATLHSTVDRTIIPLS
                     DMPLQLRHAVIAVEDKGFYDHPGIDVAGIFRAAWTDLVSGEVVQGGSTLTQQLVKNVY
                     AGQYTEDPETGVETYAVPPRTLGQKVRESLLAVKVEQEFTKDEILAKYLNTVYFGRGA
                     YGVQAAAQTFFRKDASELSALESALLAGLIQSPSYYDPIEQEEAALERRNYVLERMAS
                     EGYLTPERAAQLEGREIKVDPIELGLNFPAKLGYFLDYTRRELGDRYTEGQVFGGGLQ
                     VTTTLDSQMQRYAEEAVANQLDTPGDPAAALVAIDPRDGAVRAMYGGANFNVSKVNLA
                     TGDGGSGRQAGSAFKPFTLATAIEQGYSLNSRWHGPSSITIPDPACYTHGAPWELSNA
                     ADEESGDFTLLSATTYSVNTVFAQVASAVTPEAIRQTATRMGIRSPLEPVCSITLGTQ
                     AVTPLEMTNAYATLAARGWRHRASPLERVDTADGETDLQIGGRGKLVLRQNDADLVTY
                     ALQSVIQSGTGTAADIGRPAAGKTGTAQEYRDAWFCGYVPQLVTCVWVGYPQAEISLT
                     NVEGFSAVFGGTIPALIWHDFMSQATENLPIKDFAQPSFEGYTLGAPTPAPVTSSPSP
                     KPSPSPSRSPRPSPSPSPQPSPSPSPPPSPSPSPSPSPPPSPSPSPSPSPPASPSPTL
                     SPSAGLPERPAPLDSS"
     gene            complement(3809..>3988)
                     /locus_tag="VIH70_05035"
     CDS             complement(3809..>3988)
                     /locus_tag="VIH70_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8115759.1"
                     /translation="RVGLASMRERVELANGTFMVHSAPGRGTTVVASLPMGAVPAARE
                     FSGDDESGPATDPDP"
BASE COUNT          583 a         1456 c         1378 g          571 t
ORIGIN      
        1 aggaggcggg gcagacgacg gcggagtacg cgctggtgat cctcgccgcc gccgcgatcg
       61 ccgtcgtgtt gatcgcgtgg gcgcgatcgt cgggcaagct gccggcgttc ttcgactcca
      121 tcatcgatca gatcgccgga agtgcgggct gatcgcgtgc gcgagcgcgg ttccgcggcg
      181 gtcgagttcg cgctgctgtt gccgatcctc ctcctcctgt tgctggcgct cgtgcaggtc
      241 ggggtcatcg cccgcgactc cctcgtgctc acgcaggcct cgcgcgcggg ggcgcgtgag
      301 gccgccgtgc aggggaccat ggaggcggtc gacgaggcgg tccgcgcggc cgccgtcggt
      361 ctggatcccg atcggatcag cgtggtcgcg acgtggagcg gggctcgggg cgccccagtg
      421 acggtcgacg tggcgtacga ggcgccggtc gcgtcgctgc tggccggatg gttgctcccc
      481 gcatccgtgt cgctgcgggc gagcgcgacg atgcggcagg agttcgcgtg accgccgacc
      541 ggccccgaca cggcgcagtg gtcgcccgcg cctcgccgac tcctcggctg acgagcgatc
      601 gtgggtcgat cacgctgctg agtgcggtgg tgctggcagc cttggtcgtg ctcgcgatgg
      661 gcgcctcgga tgtcgcccgg gtgctgcacg cggcgtcgcg agcgcagacg gcggcggacg
      721 ccgcagccct cgccgccgcg caagcgctcg cgatcgacga ggagggcccc gaaccggcgg
      781 cgcttgccgg cgagtacgcc gagcggaacg gtgccgtgct cgagacgtgt tcgtgcgagc
      841 gcgggacgtt cgaggcgacg gtcagcgtgc gtctgcccgt gggcgacctc ttcctggtcg
      901 ccggggatcg gacggtgcag gccagggctc gggcccaggt ggacctgccc gctccctgac
      961 ctgcgaaggc ctccctgacc tgcgaaggtc gaacccgcct ccgtcgggag gcgggtgtac
     1021 catgcctcga cgatggctat gcgacaacgg ctcccggtca ggcgcggccg ccgatcgctg
     1081 ctcgcgacgg tggactactc gctcgccaag cgagccctga ttaaccaggt ccggaccgga
     1141 ctcgcctcgt ggaccgacat ctgcgacgcc catcccgagt tgatgcgggc cgcccagcac
     1201 gtgggcgagc cgacgcggag ggaatgcccg gtgtgccacg acgccaagct caagctcgtg
     1261 acgtacgtct acggggacgg gctcaagcac gacaacgggc gcgtgtggcc actcgacgtc
     1321 gggctcgaca tggccgccga ccggcccggc tcctggtgtt acgtggtcga ggtctgcacc
     1381 gagtgccact ggaaccacct gcacgaggcc ttcgtggccc gcgcgggctg atcgccctcc
     1441 caagactcca cgccaagact ccacgagagc ttcacgacgt ttccgcaggt cgcgtcccgg
     1501 ggaatgcgga cccgtgacta cacttgacga cgagttggcg gtcgagaccg cgccaccgcg
     1561 cgcgcccagg ggtcgtaggc acgcccccac caagaagcgg aagggattca aggcctggct
     1621 ccgccggtgg tggtgggtgt tcgtcgtcat gcccctggtc gccgccatcg gcctgctgct
     1681 gacgttgttc tacatctact cgcagctcga cctgcccaag acgccgccgc cgttgcagac
     1741 gacctacatc tacgatcgcg acggccatca gatcgcgacg ctgcactcga cggtcgaccg
     1801 cacgatcatc ccgctgtcgg acatgccgct ccagctccgg cacgccgtga tcgccgtcga
     1861 ggacaagggc ttctacgacc acccgggcat cgacgtcgcc gggatcttcc gcgctgcgtg
     1921 gaccgacctc gtctccggcg aggtcgtgca gggcggttcg acgctcaccc agcagctcgt
     1981 gaagaacgtg tacgcgggcc agtacacgga agatcccgag accggcgtcg aaacctatgc
     2041 ggtcccgccg cggacgctcg ggcagaaggt gcgcgaaagc ctgctcgcgg tcaaggtcga
     2101 gcaagagttc accaaggacg agatcctggc caagtacctg aacaccgtct acttcggccg
     2161 cggcgcgtac ggcgtccagg ccgcggcgca gacgttcttc cgcaaggacg cctccgagct
     2221 ctcggcgctc gagtccgcgc tgctcgccgg cttgatccag agcccctcct actacgaccc
     2281 gatcgagcag gaggaggccg cgctcgagcg ccggaactac gtcctcgagc ggatggcctc
     2341 cgagggctac ctcaccccgg agcgcgcggc gcagctcgag ggccgggaga tcaaggtcga
     2401 cccgatcgag ctcggcctga acttcccggc caagctcggc tacttcctcg actacacgcg
     2461 gcgggagctg ggcgatcggt acaccgaggg ccaggtgttc gggggcgggc tccaggtcac
     2521 gacgaccctc gactcgcaga tgcagcgcta cgccgaggag gccgtcgcca accaactcga
     2581 cacgccgggc gacccggcag cggcgctcgt cgcgatcgac ccgcgggacg gggccgtccg
     2641 cgcgatgtac ggcggcgcga acttcaacgt gtcgaaggtg aacctcgcga ccggcgacgg
     2701 cggttcggga cggcaggccg ggtccgcgtt caagccgttc acgctcgcca ccgcgatcga
     2761 gcaggggtac tcgctcaact cgcgctggca cggcccgtcc tcgatcacga tccccgaccc
     2821 cgcctgctat acccacggcg caccatggga gctctcgaac gcggccgacg aggagagcgg
     2881 cgacttcacc ttgctgtcgg ccaccacgta ttcggtgaac accgtcttcg cgcaggtcgc
     2941 gtcggccgtg acgccggagg cgatcaggca gacggcgacc cggatgggga tccgatcgcc
     3001 cctcgaaccg gtgtgctcga tcacgctcgg gacccaggcg gtcacgccgc tcgagatgac
     3061 aaacgcatac gcgacgctcg ccgcccgcgg atggcggcac cgggcctcgc cgctcgagcg
     3121 ggtcgacacg gcggacggcg agaccgacct gcagatcggc gggcggggca agctggtcct
     3181 gcgccagaac gacgccgacc tcgtcaccta cgccctgcag agcgtgatcc aaagcggcac
     3241 cggcaccgcg gccgacatcg ggcgtccggc cgccggcaag accggcaccg cgcaggagta
     3301 tcgggacgcc tggttctgcg ggtacgtgcc gcagctcgtg acgtgcgtgt gggtgggcta
     3361 cccgcaggcc gagatctcgt tgacgaacgt cgaggggttc tcggcggtgt tcggcggcac
     3421 gatccccgcg ctgatctggc acgacttcat gtcgcaggcc accgagaacc tgccgatcaa
     3481 ggacttcgct cagccgtcgt tcgaggggta cacgctcggc gcgccgaccc cggcgccggt
     3541 cacctcatcg ccctctccga agccgagtcc gtccccgtcg cggtcgcccc ggccgagccc
     3601 gtcgccgtcg ccccagccga gcccctcgcc gagcccaccg ccgagcccct cgccgagccc
     3661 ctcgccgagc ccaccgccga gcccctcgcc gagcccgtcg ccgagccccc cggcctcacc
     3721 gtcgccgacc ctgtcgccgt cggccgggct ccccgagcgc cccgcgccac tcgactcgag
     3781 ctgacgcggg gatcctccgc ccgctcgcct acggatcggg gtcggtcgcc ggtcccgatt
     3841 cgtcgtcgcc cgagaactcc cgtgccgccg gcacggcgcc catcggcaac gaggcgacca
     3901 cggtcgtgcc cctgccgggt gccgagtgca ccatgaacgt cccgttcgcg agctcgaccc
     3961 gttcgcgcat cgaggccaga ccgacccg
//
LOCUS       DATBRK010000168         6147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2191751, whole genome shotgun sequence.
ACCESSION   DATBRK010000168 DATBRK010000000
VERSION     DATBRK010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191751"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1059)
                     /locus_tag="VIH70_05040"
     CDS             complement(190..1059)
                     /locus_tag="VIH70_05040"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HEY8115760.1"
                     /translation="MAGTASTVRSWLLLEDPGPWGRDALRDARLPERVGLELKRRCHA
                     AGVRPLLIRRASSNASAADGLACFAIRSGPEPPWIERIRLAKVTDALDLDLPPLGRGV
                     RPGFEPVDGPLFLVCTHGRRDVCCAERGRPLAQAFAAAVPGAAWESSHVGGDRFAGNL
                     VAFPHGLYLGRVRPDEAAELARAYADGRVSPRHLRGRSCYAMPVQAAEHALRTREGLD
                     RVDDVGLERTERRPGVSTSTFRTPMGRFSVSVAIEESAPSFLTCRSLAAETAPAYRTI
                     AIVRRPDLDGGAS"
     gene            complement(1118..2308)
                     /locus_tag="VIH70_05045"
     CDS             complement(1118..2308)
                     /locus_tag="VIH70_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8115761.1"
                     /translation="MTSVAELERTSGSRRPALARCIGDADRFLQGTWGRRASIHEGGD
                     PLGFADLLTLDDVDRILSTTSLRTPAFRLVLAGEQIPETAYTRSGTTGSRPVAGMADP
                     ARVFELFRDGATIVLQGLHRYHEPVTRFCRELEVELGHPCQVNAYVTPAGAQGLDLHD
                     DPHDVFVLQAFGRKSWEIHTAPGEDEREPLRNEIGPGDCIYMPTRTPHAASTQRSLSG
                     HLTVGVHVVAWRELVRAALELIAADPSLDEPIPPGWPADLEGLERSLRERLDAAGDAL
                     EAVDAGDIVDARFERFLSTRPPLIRGALLDQGGLDGIADGSRLARRDGSICVLRIRGD
                     RLVALLGDRRLEMPAWLEPAMRRVAATPLFMVGDLADFLPDPGARAVLARRLVREGLL
                     RPLP"
     gene            complement(2305..2562)
                     /locus_tag="VIH70_05050"
     CDS             complement(2305..2562)
                     /locus_tag="VIH70_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005452439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115762.1"
                     /translation="MDEATTGDRTLSDEDILTVAPSASSAETERPMKDDTDSQDADGR
                     DGDATDQQDGDAKDQQDADGTDAQDSDTTDSKDTDGTDAAS"
     gene            complement(2618..3643)
                     /locus_tag="VIH70_05055"
     CDS             complement(2618..3643)
                     /locus_tag="VIH70_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY8115763.1"
                     /translation="MGGDPDGERGSGERVNAEVLALAADLSRRGRPFVLATVVWRRAP
                     SSGKEGSTALITPDRRVRGWLGGACAEPMVIREALRALEEGTSRLLFLGGEEELLLEA
                     RGREGVVTVPIACQSEGALEVHVEPVLPQPHLVVIGRSPAVGTLATMAGALGWRTLVV
                     DEGGLAEDYPGADRVLTSLELSDADVTDRSVIVVATQGHYDEAALEAALATPAAYVGL
                     VASRRRAGAVLGYLRDRGATDDQIARVHAPAGLDLGKVAHEEIAVAILAELVRERASG
                     GLAAASPAEVPTHEAIDPVCGMTVDVASAVFRSTYEDATYYFCSAGCLQRFREDPARF
                     VASVRGI"
     gene            complement(3598..5961)
                     /locus_tag="VIH70_05060"
     CDS             complement(3598..5961)
                     /locus_tag="VIH70_05060"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEY8115764.1"
                     /translation="MAAEAEEIRGIGHSVRRIEDDRFIRGAGNYIDDIHLPGMLYMAI
                     LRSPYAHAKLNGIDASAAQALDGVIAVVTGELMAQHNLAWMPTLSGDTEAVLVTDKVR
                     FQGQEVAAVIATDPYVAKDALELIDVDYEPLGVVVSPDQAKAAGAPLIRDEKEGQTDN
                     HIYHWESGDEESTDTAFAQADRVVSLDTFYPRSHPSPLETCGCIADVNPATGQATIYM
                     TSQAPQLIRTVFALVAGLPEEKIRIVSPDIGGGFGNKVPVYPGYVIATAASLLLGRPV
                     KWIEERSENLISTGFARDFHMHGEMALKADGTMTGLRVKLDADEGAFHADAQPSKLKV
                     GLFHIVSGSYDLPTAHVTADGYYTNKAPGGVAYRCSFRVTEASYMIERLVQNAAYEIG
                     MDPAEFRKKNFIEKDQFPYRSATGFEYDSGDYQGAMDLALEKVGYEQLRKEQADAREQ
                     GRLIGIGLASFTEVVGAGPHKDYDILGIKMNDGAELRVHPTGKAILKISSQTQGQGHE
                     TTFAQIVAEELGIQPEDIKVMHGDSDDTPYGLGTYASRSTPTVGAATAMVSRKLRDKA
                     RKLAAHLLEVSEEDVEFEKGRFTVKGAPDKAVTIQDVAFAAYTNFPDGMEAGLEGVTY
                     YDPPNLTFPFGTYAVVVEVDADTGVWKVDRVVAVDDCGVRINPMIVEGQIHGGLCEGF
                     GIAAMEQITFDEEGNCIGANFLDYLLPTAWETPRFELGETVTPSPHHPIGAKGVGESA
                     TVGSPAAFVNAVIDALWPLGVRNIDMPLTSAKVWEAIQTASAGAASG"
     gene            complement(5974..>6147)
                     /locus_tag="VIH70_05065"
     CDS             complement(5974..>6147)
                     /locus_tag="VIH70_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8115765.1"
                     /translation="SALLAENPTPSEDEVRWAISGNICRCTGYMNIVKAIQAAGRDMA
                     AGSAEIADATVAS"
BASE COUNT          823 a         2248 c         2148 g          928 t
ORIGIN      
        1 agccgggcgc ccgggcgacg gtaggtcgag ccgctcacgg ccacgatcgt cgcgagcgcg
       61 agcttgctcc cgcgcgccga gagcgactcg atcgccgaca ggacgtcggt gagctcgctc
      121 atgggtccga tggtacgcgc gcggcgcggt tcggacctgg tcgcgcgtcg ctcgtcacgg
      181 cggccgtcgt caggaggcgc ccccgtcgag gtcgggccgg cgcacgatgg cgatcgtccg
      241 gtacgcgggg gcggtttcgg cggcgaggct gcggcacgtg aggaagctcg gggccgactc
      301 ctcgatcgcg acggagaccg agaagcgccc catcggtgtt cggaaggtgg aggtcgacac
      361 gcctgggcgc ctctcggtgc gttcgagccc gacgtcgtcg acgcggtcga gcccctcgcg
      421 cgtccggagg gcgtgctcgg ccgcctggac cggcatcgcg tagcacgacc gtccgcgcag
      481 gtgccgcggg gagaccctcc cgtccgcgta cgcgcgggcc agctcggccg cctcgtccgg
      541 ccgcacgcgg cccaggtaca gcccgtgcgg gaacgcgacg aggttacccg cgaaccggtc
      601 gccgccgacg tggctgcttt cccacgcggc gccggggacg gcggcggcga aggcctgcgc
      661 gagcggacgg ccgcgctcgg cgcagcagac gtcgcgacgc ccgtgcgtgc acaccaggaa
      721 gagcggtccg tcgaccggct cgaacccggg ccggacgccc cggccgagcg ggggtagatc
      781 gaggtcgagg gcgtccgtga ccttcgcgag ccggatgcgc tcgatccacg ggggctcggg
      841 tcccgaacgg atcgcgaagc acgcgaggcc atccgcggcc gaggcgttcg aggacgcgcg
      901 gcggatcagg agcgggcgga cgcccgcggc gtggcaccgg cgcttgagct cgagccccac
      961 gcgttcgggc agtcgcgcgt cgcgcagcgc gtcccgcccc cagggtccgg gatcctcgag
     1021 caggagccag gaccggaccg tcgacgcggt gcccgccatc ggctcatcgc gtgcctcgct
     1081 cgcctgcgag caccgttcgg accgcgtcag cacacgctca gggtagaggg cggagcaatc
     1141 cctcccgcac gagccggcgc gcgagcaccg cccgggcccc gggatcgggc aggaagtccg
     1201 cgaggtcccc gaccatgaac aacggtgtcg ccgcgactcg gcgcatcgcg ggctcgagcc
     1261 aggcgggcat ctcgagccgg cgatcgccga ggagcgcgac gaggcggtcg ccgcggatcc
     1321 ggagcacgca gatcgaaccg tcccggcggg cgagccgcga cccgtccgcg atcccatcga
     1381 gccccccctg gtcgaggagc gcgccacgga tcagcggcgg ccgggtcgac aggaagcgtt
     1441 cgaaccgcgc gtccacgatg tcgcccgcat cgacggcctc cagcgcgtcg cccgctgcgt
     1501 cgagccgctc ccggagcgat cgttcgagcc cctcgaggtc ggccggccac cccggcggga
     1561 tcggttcgtc cagcgacggg tccgcggcga tcagctcgag cgcggcgcgt acgagctcgc
     1621 gccacgccac gacgtggacg cccacggtga ggtgcccgga cagcgaccgc tgggtcgacg
     1681 ccgcgtgcgg ggtccgggtc ggcatgtaga tgcagtcgcc ggggccgatc tcgttgcgga
     1741 gcggttcgcg ctcgtcctcg cccggtgcgg tgtggatctc ccacgacttg cgtccgaacg
     1801 cctgcagcac gaacacgtcg tgcgggtcgt cgtggaggtc gaggccctgc gcgcccgccg
     1861 gcgtcacgta cgcgttgacc tggcacgggt gaccgagctc gacctcgagc tcgcggcaga
     1921 agcgggtgac gggctcgtgg tagcggtgca ggccctgcaa cacgatcgtc gccccgtccc
     1981 ggaacaactc gaagacccgg gcggggtccg ccatgcccgc gaccggcctc gaacccgtcg
     2041 tgcccgaacg cgtgtacgcc gtctcgggga tctgctcgcc cgccaggacc agacggaacg
     2101 cgggcgttcg aagcgaggtg gtcgagagga tccggtcgac gtcgtcgagc gtcagcaggt
     2161 cggcgaaccc gagcggatcg ccgccctcgt ggatcgaggc ccgccgcccc cacgtccctt
     2221 gcaggaagcg atcggcgtca ccgatgcatc gtgcgagcgc cggtcgtcgc gagccgctgg
     2281 tccgctcgag ctcggcgacc gaggtcacga cgccgcgtcg gtcccgtccg tgtccttcga
     2341 gtcggtcgtg tcggagtcct gcgcgtccgt gccgtccgcg tcctgctggt ccttggcgtc
     2401 gccgtcctgc tggtccgtcg cgtcgccgtc gcggccatcc gcgtcctgcg agtcggtgtc
     2461 gtccttcatc ggccgctcgg tctcggccga gctcgccgac ggtgccacgg tcaggatgtc
     2521 ctcgtccgac agcgtcctgt cgccggtcgt cgcctcatcc atcggtcgtc ccctccttcg
     2581 tgcgtcaccg cgatcacccg acaggtgtcg cgtagcctca gataccccgt acgctcgcca
     2641 cgaacctcgc ggggtcttcc cggaaccgct gcaggcaccc ggcggaacag aagtagtagg
     2701 tcgcgtcctc gtaggtcgac cggaacacgg ccgaggcgac gtccaccgtc atcccgcaca
     2761 ccgggtcgat cgcctcgtgc gtggggacct cggccggcga agcggccgcc agcccaccgg
     2821 aggcgcgctc ccgcacgagc tcggcgagga tcgccaccgc gatctcctcg tgtgcgacct
     2881 tgcccaggtc caggccggcc ggcgcgtgga cgcgggcgat ctggtcgtcg gtcgcgccgc
     2941 ggtcgcgcag gtacccgagc acggcgccgg cgcgcctgcg cgaggcgacg aggcccacgt
     3001 acgcggcggg ggtcgcgagc gcggcctcca gcgcggcctc gtcgtagtgc ccctgcgtcg
     3061 ccacgacgat caccgagcga tcggtcacgt cggcgtcgga gagctcgaga gacgtgagga
     3121 cgcggtccgc gccggggtag tcctcggcga gtccgccctc gtccaccacg agcgttcgcc
     3181 accccagggc tccggccatg gtggccagcg tcccgacggc cggcgagcga ccgatgacga
     3241 ccaggtgcgg ttgcggcagc acgggttcca cgtggacctc caaggctccc tcgctctggc
     3301 aggcgatcgg cacggtgacg acgccctcgc ggccccgcgc ctcgagcaac aattcttcct
     3361 ccccacccag gaacagcaac cgcgacgtgc cctcctcgag cgcccgcagc gcctcgcgga
     3421 tcaccatggg ttcggcgcac gcgcccccga gccagccgcg gacgcgacga tcgggcgtga
     3481 tgagcgcggt cgacccctcc ttgcccgagg agggcgcccg gcgccacacg acggtcgcga
     3541 gcacgaacgg ccggccccga cgcgagaggt ccgccgcgag cgcaaggacc tcggcgttca
     3601 cccgctcgcc gctcccgcgc tcgccgtctg gatcgcctcc cacaccttcg cgctcgtgag
     3661 cggcatgtcg atgttgcgca cgccgagcgg ccacagcgcg tcgatcaccg cgttgacgaa
     3721 cgcggccggg gatcccaccg tggccgactc gccgaccccc ttggcgccga tcgggtggtg
     3781 gggcgagggc gtcacggtct cgccgagctc gaaccgcggc gtctcccacg ccgtcggcag
     3841 caggtagtcg aggaagttgg cgccgatgca gttgccctcc tcgtcgaacg tgatctgctc
     3901 catcgcggcg atcccgaacc cctcgcacag gccgccgtgg atctggccct ccacgatcat
     3961 cgggttgatg cggacgccac agtcgtcgac cgcgaccacg cggtcgacct tccacacgcc
     4021 cgtgtccgcg tccacctcga cgaccaccgc gtaggtcccg aacgggaacg tgaggttcgg
     4081 cgggtcgtag taggtcacgc cctccaggcc ggcctccatg ccgtcgggga agttcgtgta
     4141 cgcggcaaac gccacgtcct ggatcgtgac ggccttgtcc ggcgccccct tcacggtgaa
     4201 tctccccttc tcgaactcga cgtcctcctc ggacacctcg aggaggtggg cggcgagctt
     4261 gcgggcctta tcccggagct ttcgcgagac catcgccgtg gccgcgccga ctgtgggtgt
     4321 cgaacgcgat gcgtacgtcc caagcccgta gggcgtgtcg tccgagtcac cgtgcatcac
     4381 ctttatgtcc tcgggctgga tgccgagctc ctccgccacg atctgggcga acgtcgtctc
     4441 gtgcccctga ccctgcgtct gcgagctgat cttcaggatc gccttgcccg tggggtgcac
     4501 gcggagctcg gccccgtcgt tcatcttgat cccgaggatg tcgtagtcct tgtgcgggcc
     4561 ggccccgacg acctcggtga agctcgcgag gccgatcccg atcagccggc cctgctcgcg
     4621 cgcgtccgcc tgctccttgc ggagctgctc gtacccgacc ttctcgagcg cgagatccat
     4681 cgcgccctgg tagtcgccgg agtcgtactc gaagcccgtg gccgacctgt acgggaactg
     4741 gtccttctcg atgaagttct tcttccggaa ctcggccggg tccatgccga tctcgtacgc
     4801 ggcgttctgt acgagccgct cgatcatgta ggaggcctcg gtcacccgga acgagcatcg
     4861 gtacgcgacg ccgcccgggg ccttgttcgt gtagtagccg tcggccgtga cgtgcgcggt
     4921 ggggaggtcg taggaaccgc tcacgatgtg gaacagcccg accttgagct tcgacggctg
     4981 ggcgtcggcg tggaacgcgc cctcgtcggc gtcgagcttg acgcgcaggc cggtcatcgt
     5041 cccatcggcc ttcaacgcca tctcgccgtg catgtggaag tcgcgcgcga accccgtcga
     5101 gatcaggttc tcgctgcgct cctcgatcca cttcacgggg cggccgagca ggagcgaggc
     5161 cgccgttgcg atcacgtacc ccggatacac ggggaccttg ttgccgaagc cgccgccgat
     5221 gtccggcgag acgatccgga tcttctcctc cggaaggccg gcgacgagcg cgaacacggt
     5281 tcggatcagc tgcggggcct gcgaggtcat gtagatcgtt gcctgaccgg tcgccgggtt
     5341 cacgtccgcg atgcacccgc atgtctccag cggcgacggg tgcgagcgcg ggtagaacgt
     5401 gtcgagggac acgaccctgt cggcctgcgc gaacgccgtg tccgtggact cctcgtcccc
     5461 ggactcccag tggtagatgt ggttgtcggt ctggccttcc ttctcgtcgc ggatcagcgg
     5521 tgcgccggcc gccttcgcct ggtccggcga caccacgacc ccgaggggct cgtagtcgac
     5581 gtcgatcagc tcgagggcgt ccttcgcgac gtacggatcg gtcgcgatca ccgccgcgac
     5641 ctcctgcccc tggaaccgca ccttgtcggt gacgagcacc gcctccgtgt cccccgacag
     5701 cgtcggcatc cacgcgaggt tgtgctgtgc catcagctca cccgtgacga cggcgatgac
     5761 cccgtcgagc gcctgcgccg ccgaggcgtc gatcccgttc agcttggcgt gcgcgtacgg
     5821 gctccgcagg atcgccatgt acagcatccc ggggaggtgg atgtcgtcga tgtagttgcc
     5881 ggcgcccctg atgaaccggt cgtcctcgat gcgccggacg ctgtgtccga tccctctgat
     5941 ctcttcggcc tcggcagcca tctgcggacc ccctcagctc gccacggtgg cgtcggcgat
     6001 ctcggccgag cccgccgcca tgtcgcggcc tgccgcctgg atcgccttga cgatgttcat
     6061 gtagccggtg caccggcaga tgttgcccga gatcgcccag cgcacctcgt cctccgacgg
     6121 cgtggggttc tcggcgagca gcgcgga
//
LOCUS       DATBRK010000169         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_890738, whole genome shotgun sequence.
ACCESSION   DATBRK010000169 DATBRK010000000
VERSION     DATBRK010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890738"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VIH70_05070"
     CDS             complement(<1..507)
                     /locus_tag="VIH70_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8115766.1"
                     /translation="MDAGRVVANRRTFDRGDIVFHQGDPGDSLHLVRQGMFAIRSSTP
                     SGAILIVDILVPGDVFGEFAVFSNQRRRTTEVTALSPGATITVEREQLRVAIGFRPEL
                     AEELIATIIGKAESTTQRLVEMLYVPAELRVLRALLSMATLEDAPSPVRLTQDELASF
                     AATTRPTAN"
     gene            733..978
                     /locus_tag="VIH70_05075"
     CDS             733..978
                     /locus_tag="VIH70_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115767.1"
                     /translation="MADHEERLTERLMVQLSPRDLRALQAIADEMERPLAWVARRGIR
                     LYISQILEAEETLARGDHIVEEMRREGREAGEQEKGR"
     gene            complement(1020..1184)
                     /locus_tag="VIH70_05080"
     CDS             complement(1020..1184)
                     /locus_tag="VIH70_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115768.1"
                     /translation="MLPDVERADRIGEFWGYPKSRAFAELLIDCEVDRVLRAVLVGML
                     REIDRPYQAH"
     gene            2259..3153
                     /locus_tag="VIH70_05085"
                     /pseudo
     CDS             2259..3153
                     /locus_tag="VIH70_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567921.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(3332..3691)
                     /locus_tag="VIH70_05090"
     CDS             complement(3332..3691)
                     /locus_tag="VIH70_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY8115769.1"
                     /translation="MQFVGAAEAADEATMRSMLAADPGLRDRHADLLARMAEARNWST
                     VRLLIDLGFGVNGVTAAGATALHHAAAAGELSIARLLVEHGADHTAREPEFNAQPAGW
                     AQYFKKAGTQKYLESLA"
BASE COUNT          703 a         1407 c         1435 g          724 t
ORIGIN      
        1 gttggcggtc ggtcgggtcg ttgcggcgaa gctcgcgagt tcgtcctggg tgagccggac
       61 cggcgacggg gcgtcttcca gggttgccat cgacagcaga gcccggagta cccggagttc
      121 cgccggcacg tagagcatct ccaccaacct ctgcgttgtg ctctcggcct ttccgatgat
      181 ggtggcgatg agctcctcgg ccagctccgg ccgaaagccg atggcaacgc ggagctgttc
      241 ccgttccacg gtgatcgtcg cgccggggga cagtgctgtg acctccgtcg tccgacgccg
      301 ctggttcgag aagaccgcga actcaccgaa cacatcgccg gggacgagga tgtcaacgat
      361 caggatcgct cccgaaggcg tggacgaccg gatcgcgaac atcccttgtc gaacgaggtg
      421 aagcgagtcc ccgggatcgc cttggtgaaa gacgatgtcc cccctatcga acgtccgccg
      481 gttggcgacg acgcggccgg cgtccacggc cgccaggaca cgctggaaca agcgttcgcc
      541 gctcgtgtgt gatggtctcc gggtctcggc ctcggaagaa atgacaggca gttgcttcat
      601 tgttcaactc tttcactcga actggaacag ggcttctagc gccatgctgt gcttcatagc
      661 gttgcacaac gccgagagta tcaggggtgt ggaagcagtg caaaggcgct gcgacggcag
      721 tagggtggtg tcatggcaga ccacgaggaa cggcttacgg aacggctgat ggtccagctg
      781 agtccgcggg acctacgcgc gctccaggcg atcgcagacg agatggagcg gcccctggca
      841 tgggtcgccc gtcggggcat ccggctgtac atctcccaga tcctcgaagc ggaggagacg
      901 ctggcccggg gtgaccacat cgtcgaggag atgcgccgcg agggacgcga ggcgggtgag
      961 caagagaaag gacggtgact ggtgaatgag atcgccggct cgtcgtgcgc cgccggctct
     1021 caatgcgcct ggtacggtcg gtcaatctcc cgcagcattc cgaccagcac cgctcggagc
     1081 acccgatcca cctcgcagtc gatcaggagt tcagcgaagg cgcggctctt tgggtagccc
     1141 cagaactcgc cgattcggtc ggcacgctcg acgtcgggga gcatcaggac gtgagcagct
     1201 cggcgcgggg ggcgggaccg agcgcgtcga gacgttctcg taagcggcgc tcaagctgat
     1261 ccggcctcat cgagacccac atgcccgtcc aaacgagaca ctcattcggt gcctggtgtc
     1321 cagggcccgg gagcgtccca cacgtatcag gaggtggccg cctacacgct cgggctgcac
     1381 gatgccgcgt tcatccacca acacatcgtc gatgcgtacg cggttcactc ggcgatgccg
     1441 accgtcaagc cgatcaggat cgcccaggcg ctggggggct gttcctgcac gccgagcacg
     1501 ggttcacggg ccagcaggtt cagcgggttc accccatcct cgcagcgcag cggcccgcct
     1561 ggccgaggtt cgcactcccg ggcgatcgcg gcccgatcac catccacgat gtcgccgccg
     1621 agccaccggg cgaacgacgt gatcgggcgg tcgaggcgtt ggcgacctcg acgtggaacg
     1681 cgtgccaagg ccttcgccgt cgatccaggc attcctctcg tcctgcggag ttacgcctcc
     1741 tgccacctga cgacaggact tcgccggcgc ggagcggatc tatccggaac ccggacccat
     1801 atgctcggtt cagcaggcgg gtcgaatcgc gctcacgcac ccaagttgca ctacctgtac
     1861 gaacgcgctc ggcggctttc gtccctttcg tctggttccg gcctggggcg caagtacgac
     1921 cctagtttgg gtcgtaggtg cgggcccaaa cgtgcgaacc cgcaggtcga gagaggcgat
     1981 tcatctcccc gcggtgacgg aggtcccggc ctctcctccc cggtcactgc cggtgaggac
     2041 gggcacgagt tcgagctcga tgttgccgtc ggggttgacg gctacgaccc gagcgatccg
     2101 cgagtcggcc tcgtcgtcac cgacgacgag gcggcaaccg acgacctatg agccgccccg
     2161 ggattcgagg aggctctgtc cagccgggcc aggcgtgtca cgttccccgt gtctgagggc
     2221 tcacctctgt gatctcaggc gatgggatgg tgtgacaggt ggacgagaac gagcggctgg
     2281 ccgagcgctt cgagcagcac cgaccccggc tgcgggcggt ggcgtatcga atgctcggct
     2341 cgctggctga cgcggacgac gctgttcagc atgcctggct gcggctgagc cggtccgacg
     2401 cggagcagat cgacaacctc ggcgggtggt tgaccaccgt cgtcgcccgg gagtgcctgc
     2461 atatgttgcg cgcccgccga cgccggcggg aggatccgtt cgccgcgcac ctgcccgacc
     2521 tggttgccat cccggacggc gacctcgacc ccgagcagga ggcgttgctg gccggccgga
     2581 gtgagctggt cgagcacgga tgagcagggc caagctcacc gagtcgagcg gttggtgttc
     2641 gtgctgcacg acatgttcga gctgcccttc gaggagatcg ccggcctcgt ggaccgcacc
     2701 ccggccgccg cacgacaact cgccagccgg gcacggcgcc gggtgcacgg tgccgaaatt
     2761 ccatcacccg atccggatct agcgcgccag cggcaggtcg tccgcgcctt ctacgcggcc
     2821 gcgtggatcg gcgacttcga cgccctcgtc caggtcctag accccgacgt tgtcctgcgc
     2881 accgacttcg gcaccacccg gccgccaacc gtcatccgcg gcgcgcaggc cgtggccaag
     2941 caggcccgcg ccccgcgcgg tggccaactg cgccccgccg tcgtcaacgg cacgatcgga
     3001 acgctgatca cccgcgacgg gcggccgttc tccgtcatga ccttcaccgt cacggccggc
     3061 aagattgtca ggatcgacgt tatccgcgac cccgaccgcg tccaccgcgt cgcgtccgcc
     3121 ctcctcaacg ggacgaagcg agcaggacgg tgaatgccgt ccacaacaac catatgcatg
     3181 cgctcgccgc gagtacacag atccagcaga tgggtggacg gcgccattgg gcatctccat
     3241 ctcgacgctc gattgaggtc aacgacagtc ggcgccgagc ggaatggcgg gcctattttc
     3301 gacgagctgg gccgcgaccg gcggggctgc atcaggccag ggattcgaga tatttctggg
     3361 ttcccgcctt cttgaagtat tgcgcccagc ccgccggctg cgcgttgaac tcgggttcgc
     3421 gggcagtgtg gtcggcaccg tgctcgacca gcaggcgcgc gatcgagagc tcgcccgctg
     3481 ccgccgcatg gtgcaaggcg gtggcgccgg cggcggtgac cccgttgacg ccgaagccaa
     3541 gatcgatcag cagccggacc gttgaccagt tgcgcgcctc ggccatccgt gcaagcaggt
     3601 ctgcgtgccg atcgcgcagc ccgggatcgg cggccagcat cgaccgcatc gtcgcttcgt
     3661 cggcggcctc ggcggctcct acgaactgca ttgcgtcctc ctcacgtacg ttgctctcgt
     3721 acctatgagc ccagacgacg agccgacgtg acagggcgag gtcgtcgcct ggcgatcatt
     3781 gtcggcgtcg ccgaattcct gccgcgattt gggtgttctg gatgagaccg aacccgccta
     3841 gcagtggttc gccgggaata agcgaagatc caggctggga gactggcgcc gggagagatc
     3901 gtggagccat cggggagggc tagctatcag ggtggcggtg tgctcggtgc ccgcctgatt
     3961 cgcctgcgac cacggctccc gacttgccga cagcttcgtc gttcataccg acggttgtga
     4021 aagggttcga ttgagcgcgt catcggtgct cgcgcttccg gtatccctct cgccgaagct
     4081 tcgcgtgacg ggcgctgaga cagagcacat gcccttctac ctgcaggtat tttttcgagt
     4141 actgacgcta tgtaggtcgg gcctcggttc cctgctcaca ggcggggctc ttacgctccg
     4201 cccgagtagt cgagctcggg aaaggaggcc cgctcatggt ctacgtcgga atggacgtgc
     4261 atcgcaagc
//
LOCUS       DATBRK010000170         6229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1617411, whole genome shotgun sequence.
ACCESSION   DATBRK010000170 DATBRK010000000
VERSION     DATBRK010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617411"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1495
                     /gene="tig"
                     /locus_tag="VIH70_05095"
     CDS             119..1495
                     /gene="tig"
                     /locus_tag="VIH70_05095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY8115770.1"
                     /translation="MQTTLEETDKHTVKLTVEIPPEEVDKDLDRAYRKIARQVKIPGF
                     RKGKVPKRVIDAQFGHDAVLGEFIEESVPTYYRDALREHDLAPITEPDIELGDVEEGK
                     PLVFTAVVEVRPRLKLEESDYRGVKVERPKVEISEDDVERYLDSLRERFAELENVSRA
                     AREGDYVGLDLHAAVHGEEIPEATRPDHLYAVGSGEFGDRLDAELVGKRAGEILAFND
                     TLGPAAGERAGQEVAFRVLVKEVKGKKLPEVDDEFAKVASEFDTLEELKADLREQLHR
                     NRERSADADVRDRVLQAMIDTVDVDLPDPLVDEEVQHRIAHAQERAEQAGMTLTQVLE
                     TQGFDELRFRADARDHSIRAIKADLVLEAVARREGIEVTAEEIGREIGSLAAAIGREP
                     KDLAKSLDRSGQVVALAGDIIRSKALDLLVEHADISPDPGAEEATQRSEGSSEEVAAT
                     GGPAEETS"
     gene            1492..2121
                     /locus_tag="VIH70_05100"
     CDS             1492..2121
                     /locus_tag="VIH70_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY8115771.1"
                     /translation="MKDYLVPVVVEQTSRGERSFDIYSRLLKERIVFLGTPIDDAVGN
                     LIMAQLLHLESEDPDKDVNLYINSPGGDITSLFAIYDTMQYIKPDVSTICMGQAASAA
                     AVLVLAGTKGKRFALPHSRILLHQPHGGAQGQAVDIEIQAKEITRYRKLLEELIAEHT
                     GQPLEKVSKDTDRDYILTADEAVEYGVVDEVITSRKIRPELAAAAAGSS"
     gene            2180..3445
                     /gene="clpX"
                     /locus_tag="VIH70_05105"
     CDS             2180..3445
                     /gene="clpX"
                     /locus_tag="VIH70_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY8115772.1"
                     /translation="MARIGDSDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNEI
                     IEEELSETSELKLEELPKPQEIYAFLDEYVIGQETAKKVLSVAVYNHYKRIQVGAKDQ
                     DVELQKSNIMILGPTGCGKTLLAQTLAKMLNVPFAIADATALTEAGYVGEDVENILLK
                     LIQAADYDVKKAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTTASV
                     PPQGGRKHPHQEFIQIDTTNVLFICGGAFAGLEKIVESRIGSRGIGFGADVRRVEDKD
                     LGAILGQVLPEDLLKYGLIPEFVGRLPVITNVHNLDKTALVDILTKPKNALVRQYHKF
                     FEFDGVELEFTDDSLGAIADQAILRATGARGLRAIMEEVLLNTMYELPSRHDVTKCVI
                     DEDVVRSKVNPTLVPVKETPKRQRQERTA"
     gene            3442..3897
                     /locus_tag="VIH70_05110"
     CDS             3442..3897
                     /locus_tag="VIH70_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY8115773.1"
                     /translation="MRLRRVAIVVPLVLVASAFSACGGDDEGTGSSDGGAQDPAALEG
                     ESWILTQMLTAGGQTEIVQTGVSAQFDGTTISGKAGCNSYHASYEASGSDISFGPIAA
                     TKKLCPEDEQSTEDRYLHLLAEIGSYEVSGRSMSMNDSDGTPVLQFMQG"
     gene            complement(3901..>6229)
                     /locus_tag="VIH70_05115"
     CDS             complement(3901..>6229)
                     /locus_tag="VIH70_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115774.1"
                     /translation="AVIDDIHWADQALLDLLEELADRVVGPAFFLCPSRPELTERRPG
                     WGGGKRNVSSVGLDPLTSAESDRLVGLLLSVADLPASVHQRILERAEGNPFFLEEIVR
                     HLIEEGVIVRDADRWRAASTIAEVQIPDTVQAVLAARIDLLDPVEKRALQRAAVVGRV
                     FWPGPVGRLLNGDREHLRSTLDRLEERDLVLSRLSSSVAGEPEFIFKHILTREVAYES
                     LPRRERAGAHAAVASWIEETAGARAKEFVELLAYHCLEAYLGARDDGLAPAEVEDLRL
                     RAFRALLGVAAEAHRRFALAKATSAIEQALRIASGPLERITALEWEGMIARSDYQGDA
                     SWRSFKEAIDLRLRHSPHDRRAIAWACVNGLENPLRWPGSMKSFPEIDEVKRYLAICA
                     DHVDDERSEEGIRLLTLRAFEPFGFGARVDIESAEREEAIAAGVRAAELAREQGRLDL
                     ESAALDGTGSAIITLGHYGRLGEMLARRLEIAEQIEDPWEAGDAYAMVAWNGAMIGDF
                     ERSVRWGLLGRDRGEGQAAGIVDHCLNWAGTGLFHLGEWDRQLEIFREVQVLMGERAE
                     EPPYFMLSLFGATAFVHEARGLPSARQLLDLLERTRGTAIEASVMASHWVAWAVARRG
                     EGDRAWSLVRDRTVESQTFRPFHDQVVAELLALTERWDEAQDFLEGSRAFAAEAGLLA
                     LPVHLDRLEARSAIASGRSEDGLERLERARDGFARLGAAWERGRTELELADAFASAGH
                     GADARMLLDAAKPDLERAGALIELERLRSLRDRLA"
BASE COUNT         1102 a         2139 c         2106 g          882 t
ORIGIN      
        1 gttcctcgtc gaacgctgtc tcgaccactg gctcgaccac cggccctccc ggcgtagggg
       61 ctccgagcga gcccgcgcgc acgataccgg atgctccttt ccgttacgct cggagcccat
      121 gcagacgacc ctcgaggaga ctgacaagca cacggtcaag ctcaccgtcg agatcccacc
      181 ggaagaggtg gacaaggatc tcgaccgcgc gtaccggaag atcgcgcggc aggtcaagat
      241 ccccggcttc cgcaagggca aggtccccaa gcgggtgatc gacgcgcagt tcggtcacga
      301 cgcggtgctg ggggaattca tcgaagagtc cgtgccgacc tactaccggg atgccctccg
      361 cgaacacgat cttgcgccga tcaccgaacc cgacatcgag ctcggcgacg tcgaggaggg
      421 caagccgctc gtgttcacgg ccgtcgtcga ggtccggccg cggctcaagc tcgaggagtc
      481 ggactacagg ggtgtgaagg tcgaacgacc gaaggtggag atcagcgagg acgatgtcga
      541 gcggtacctg gactcgctcc gtgagcggtt cgcggagctc gagaacgtgt cgcgcgccgc
      601 ccgcgaaggc gactacgtcg gcctcgacct gcacgcggcc gtgcacggcg aggagatccc
      661 cgaggccacc cgcccggacc acctgtacgc ggtcggctcc ggcgagttcg gcgacaggct
      721 cgacgccgag ctcgtcggca agcgcgccgg cgagatcctc gcgttcaacg acacgctcgg
      781 ccccgctgcg ggcgaacgcg cgggtcagga ggtggccttc cgggtgctgg tgaaggaggt
      841 caagggcaag aagctgcccg aggtcgacga cgagttcgcg aaggtggcgt cggagttcga
      901 cacgctggag gagctgaagg ccgacctccg cgagcaactg cataggaacc gggagcgatc
      961 ggcggacgcg gacgttcgcg accgggtgct ccaggcgatg atcgacacgg tggacgtcga
     1021 cctgcccgat ccgctcgtcg acgaggaggt gcagcaccgg atcgcgcacg cgcaggagcg
     1081 cgccgagcag gcggggatga cgctcaccca ggtactcgag acgcaggggt tcgacgagct
     1141 ccgattccgg gccgacgcgc gcgaccactc gatccgcgcg atcaaggccg acctcgtgct
     1201 cgaggcggtc gcgcgccggg aggggatcga ggtgaccgcc gaggagatcg gtcgggagat
     1261 cggcagcctc gccgcggcga tcggacgtga acccaaggac ctcgccaaga gtctcgatcg
     1321 gagcggtcaa gtcgtagcac tcgccggtga tatcatccgc tccaaggcgc tcgacctcct
     1381 cgtcgagcac gcggacatca gcccggaccc cggcgccgag gaggccacgc agcggtcgga
     1441 agggtcttcc gaagaggtcg cggccacggg cggaccagca gaggagacgt catgaaggac
     1501 tacctcgttc ccgtcgtcgt cgagcagacc agccgaggtg agcgctcgtt cgacatctac
     1561 tcgcgtctgc tcaaggagcg gatcgtgttc ctcgggacac cgatcgacga cgccgtcggc
     1621 aacctcatca tggcccagct cctgcacctg gaatccgagg atcccgacaa ggacgtcaac
     1681 ctgtacatca actcgccggg cggggacatc acgtcgctgt tcgcgatcta cgacacgatg
     1741 cagtacatca agccggacgt ctcgacgatc tgcatggggc aggccgcctc ggcggccgcg
     1801 gtgctcgtgc tggcggggac caagggcaag cgcttcgcgc taccgcactc gcggatcctc
     1861 ctgcaccagc cgcacggggg tgcgcagggc caggccgtcg atatcgagat ccaggccaag
     1921 gagatcaccc ggtaccgcaa gctgctcgag gagctcatcg ccgagcacac gggacagccg
     1981 ctcgagaagg tgtcgaagga cacggatcgc gactacatcc tcacggccga cgaggccgtc
     2041 gagtacggcg ttgtggacga ggtcatcacg agccgcaaga tcagacccga actcgcagcg
     2101 gcggccgccg gctcatcgta ggatttcgac ctcggacggc cgggactcgc ccggccgctc
     2161 ggctcagggg gctcagggca tggcgaggat cggcgactcg gatcttctga agtgctcgtt
     2221 ctgcggcaag tcgcagaagc aggtcaagaa gctcatcgcg ggacccggcg tctacatctg
     2281 cgacgagtgc atcgacctgt gcaacgagat catcgaggag gagctctccg agacctccga
     2341 gctgaagctc gaggagctcc ccaagccgca ggagatctac gcgttcctcg acgagtacgt
     2401 gatcggacag gagaccgcga agaaggtcct gtcggtcgcg gtctacaacc actacaagcg
     2461 gatccaggtc ggcgcgaagg atcaggacgt cgagctgcag aagtccaaca tcatgatcct
     2521 ggggccgacg gggtgcggga agacgctcct cgcgcagacg ctcgcgaaga tgttgaacgt
     2581 gccgttcgcg atcgccgacg cgaccgcgct caccgaggcc ggctacgtcg gcgaggacgt
     2641 cgagaacatc ctgctcaagt tgatccaggc cgccgactac gacgtgaaga aggccgagac
     2701 cgggatcatc tacatcgacg aggtcgacaa gatcgcccgc aagtcggaga acccgtcgat
     2761 cacgcgcgac gtctcgggtg agggcgtcca gcaagccctg ctgaagatcc tcgagggcac
     2821 gaccgcttcg gtcccgccac agggcggccg caagcacccg caccaggagt tcatccagat
     2881 cgacacgacc aacgtgctgt tcatctgcgg cggggcgttc gccggtctgg agaagatcgt
     2941 cgagagccgg atcggctccc gcgggatcgg gttcggcgcc gatgtccgac gggtcgagga
     3001 caaggacctc ggtgcgatcc tcggtcaggt gctcccggag gatctcctca agtacggcct
     3061 gatccccgag ttcgtcggcc ggctgccggt gatcaccaac gtgcacaacc tggacaagac
     3121 cgcgctcgtc gacatcctga ccaaaccgaa gaacgcgctc gtccgccagt accacaagtt
     3181 cttcgagttc gacggcgtcg agctcgagtt cacggacgat tccctcggcg cgatcgccga
     3241 ccaggcgatc ctgcgcgcga ccggtgcgcg cggtctgcgc gccatcatgg aggaggtcct
     3301 gctcaacacg atgtacgagc tgccctcccg ccacgacgtg accaagtgcg tgatcgatga
     3361 ggacgtcgtt cggagcaagg tgaaccccac gctggtgccg gtgaaggaga ccccgaagcg
     3421 gcagcgccag gaacgcactg cgtgaggctg cgccgggtcg cgatcgtcgt tccgctcgtc
     3481 ctggtcgcgt ccgcgttctc ggcgtgtggt ggcgacgacg aggggacggg ctcgagtgat
     3541 ggaggcgccc aggacccggc ggcgctcgag ggtgagtcct ggatcctcac gcagatgctg
     3601 acggccggag gccagaccga gatcgtgcag acaggggtca gcgcccagtt cgacggcacg
     3661 acgatcagcg gcaaggccgg gtgcaactcg tatcacgcgt cgtacgaggc cagcgggagc
     3721 gacatctcgt tcggtccgat cgccgccacg aagaagctct gtcccgagga tgagcagagc
     3781 accgaggatc gataccttca cctgctcgcg gagatcggct cctacgaggt gagcggccgt
     3841 tcgatgtcga tgaacgactc cgacggcacg ccggtcttgc agttcatgca gggctgagcc
     3901 tcaggcaagc cggtcccgga gcgagcgcag ccgctcgagc tcgatgaggg cgcccgcgcg
     3961 ctcgagatcc ggctttgcgg cgtcgagcag catccgcgcg tccgccccat ggcccgcaga
     4021 cgcgaacgcg tcggccagct cgagctccgt cctgccgcgc tcccaggcgg ccccgagacg
     4081 tgcgaacccg tcgcgcgcgc gttccagcct ctcgagccca tcctccgacc gaccggaggc
     4141 gatcgccgac cgcgcctcga ggcgatccag gtgcacgggc agggcgagca ggcccgcctc
     4201 ggctgcgaag gcgcggctgc cctcgaggaa gtcctgggcc tcgtcccagc gctcggtcag
     4261 ggcgaggagc tccgcgacga cctgatcgtg gaaagggcgg aaggtctgcg actccaccgt
     4321 gcggtcgcgg acgagcgacc atgcccggtc gccctccccg cgacgggcca cggcccaggc
     4381 gacccaatgg gaggccatca cggatgcttc gatcgccgtg cctcgcgtcc gctcgaggag
     4441 atccaggagc tgtcgcgcgc tcggcaagcc cctggcctca tgcacgaacg ccgtggcccc
     4501 gaacaggctc agcatgaagt acggaggctc ctccgcgcgc tcgcccatca ggacctggac
     4561 ctcgcggaag atctcgagct gccgatccca ctccccgaga tggaacagtc cggtgccggc
     4621 ccaattcagg cagtggtcga cgatcccggc ggcctggccc tcgccgcggt cccgcccgag
     4681 gagaccccat cggaccgatc tctcgaaatc cccgatcatc gcgccgttcc acgcgaccat
     4741 cgcgtacgcg tcgccggcct cccatggatc ctcgatctgc tcggcgatct ccagtcgcct
     4801 ggccagcatc tccccgagcc gtccgtagtg gccaagcgtg atgatggcgg atccggtccc
     4861 gtcgagcgcc gccgactcga gatcgagccg cccctgctca cgggcaagct cggcggcccg
     4921 gaccccggcc gcgatggctt cctcccgctc ggccgactcg atgtcgaccc gggccccgaa
     4981 cccgaacggt tcgaacgcgc gcaaggtgag caggcggatc ccctcctcgc tccgctcgtc
     5041 atcgacgtgg tcggcgcaga tcgccaggta gcgcttcacc tcgtcgatct cggggaacga
     5101 cttcatcgat cccggccatc tgagcgggtt ctcgagcccg ttcacgcagg cccacgcgat
     5161 cgcacggcgg tcatgtgggc tgtgccggag tcgcagatcg atcgcctctt tgaacgatcg
     5221 ccacgacgcg tcaccctggt agtcgctgcg ggcgatcatc ccctcccact cgagggcggt
     5281 gatccgctcg agcggccccg acgcgatccg gagcgcctgc tcgatcgcgg aggtggcctt
     5341 cgcgagcgcg aaccgacgat gggcctcggc cgcgacgccc aggagcgccc ggaacgcccg
     5401 gaggcgcagg tcctcgacct ccgccggcgc gagcccgtcg tcgcgcgccc cgaggtaggc
     5461 ctcgagacag tggtaggcga ggagctcgac gaactccttg gcgcgcgcac ccgcggtctc
     5521 ctcgatccaa gacgcgaccg ccgcgtgtgc gcccgcccgc tcgcgccggg gaaggctctc
     5581 gtaggcgacc tctcgggtca ggatgtgttt gaagatgaac tccggctcgc ccgcgaccga
     5641 cgacgagagg cgcgacagga ccagatcgcg ctcctccagt cggtcgagcg tcgaacgcag
     5701 gtgctcccgg tccccgttca gcaggcgtcc gaccggtccg ggccagaaca cgcgaccgac
     5761 gacggcggcg cgctggaggg cgcgcttttc cacgggatcc aacaggtcga tcctggccgc
     5821 gaggacggcc tgcacggtgt ccgggatctg cacctccgcg atcgtcgacg ccgcgcgcca
     5881 ccgatccgcg tcccggacga tcacgccctc ttcgatcaga tgacggacga tctcctcgag
     5941 gaagaagggg ttcccctcgg cgcgctcgag gatccgttgg tgcacggacg ccggcaggtc
     6001 cgccaccgag agcaacagcc cgacgagccg gtccgattcc gcggaggtga gcggatcgag
     6061 gccgaccgag gagacgttgc gcttgccccc gccccaaccg ggacgccgct cggtcagctc
     6121 cgggcgtgac ggacacagga agaacgccgg ccccacgacg cggtcggcga gctcctccag
     6181 cagatcgagc agcgcctggt cggcccagtg gatgtcgtcg atcaccgcg
//
LOCUS       DATBRK010000171         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_172243, whole genome shotgun sequence.
ACCESSION   DATBRK010000171 DATBRK010000000
VERSION     DATBRK010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172243"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1533)
                     /gene="dnaE"
                     /locus_tag="VIH70_05120"
     CDS             complement(<1..>1533)
                     /gene="dnaE"
                     /locus_tag="VIH70_05120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY8115775.1"
                     /translation="VADIVADIKAMGIRCACRGSAAGSLVCYLTGISDVDAVRHDLVF
                     ERFLNPLRDELPDIDVDVESARREDVYDMILSRYGQDRCACVTMVDTYRARSSIREVG
                     KALGYPEVEIDTVAKAFPHIGAQDIRKAIEILPELAGSNLAAGQLELLFRVAERLEGF
                     PRHLALHPSGIVLSDDDLVNRVPLERSFQGYRMIQADKDDVEVLGYLKLDVLGVRMLS
                     SMRHALDEIARTEALKVDLDQIPLDDWPTFELIRASDTLGCFQIESPGQRELLQKLQP
                     TRWEDLIVDISLFRPGPVKSDMIRPYLNRRHGMERPVYAHPALRLALRETFGVIVYHE
                     QVMRTLAALAGYDLTTADRIRRHLDDDAELPSLREDFLNRARERGVPDDVAETTWDDV
                     AQFASFGFCKAHAAAFAVPTYQSAYLKAHFPAHFLAGVLTHDPGMYPRRLILEDAREH
                     GIEILPLDVNVSEPDYVAERVGASYGIRLALKDVHGISDAEVRSIQEARAERPFRDVG
                     DFL"
BASE COUNT          245 a          498 c          553 g          237 t
ORIGIN      
        1 aggaagtcgc cgacatcccg gaacggccgt tcggcccggg cctcctggat ggaccggacc
       61 tccgcgtcgg agatcccgtg cacgtccttc agcgccagcc ggatcccgta gctcgcgccg
      121 acgcgctcgg ccacgtagtc gggttcgctc acgttcacgt cgagcgggag gatctcgatg
      181 ccgtgctccc tggcgtcttc caggatcagc cgccgagggt acatgcccgg atcgtgggtc
      241 agcacaccgg ccaggaagtg cgcggggaag tgggccttca ggtacgccga ttggtaggtg
      301 ggaacggcga acgccgcggc gtgggccttg cagaacccga acgaggcgaa ctgcgcgacg
      361 tcgtcccacg tcgtctcggc cacgtcgtcg gggacacccc gttctcgtgc acgattcagg
      421 aagtcctcac ggagcgacgg gagctccgcg tcgtcgtcca ggtgccggcg gatgcggtcg
      481 gccgtcgtga ggtcgtagcc ggcgagggcg gccagggtcc gcatgacctg ctcgtggtag
      541 acgatcacgc cgaaggtctc gcggagcgcc agccggagcg ccgggtgcgc gtagaccggc
      601 cgttccatcc cgtgccgacg gttcaggtat ggacggatca tgtccgactt caccggaccc
      661 gggcggaaca gcgagatgtc gacgatcagg tcctcccacc gggtcggctg gagcttctgg
      721 agcagttccc gctggccggg cgactcgatc tggaagcagc cgagggtgtc ggaggcccgg
      781 atcagctcga acgtcggcca gtcatcgagc gggatctggt cgagatcgac cttcagtgcc
      841 tcggtccggg cgatctcgtc cagggcatgg cgcatcgacg agagcatccg gaccccgagg
      901 acgtcgagct tcaggtaccc gaggacctcg acgtcgtcct tgtcggcctg gatcatccgg
      961 tagccctgga acgaccgctc gagcggcacc cggttcacca ggtcgtcgtc cgacaggacg
     1021 atgccggagg ggtggagcgc gaggtgccgg gggaagccct ccagccgctc ggccacccgg
     1081 aacaggagct cgagctgtcc ggcggccagg ttcgagccgg cgagctcggg gaggatctcg
     1141 atggccttgc ggatgtcctg ggccccgatg tgggggaagg ccttggccac cgtgtcgatc
     1201 tcgacctccg ggtacccaag agccttgccc acctcgcgga tggacgagcg ggcccgatac
     1261 gtgtcgacca tggtcacgca ggcgcaccgg tcctggccgt atcgcgacag gatcatgtcg
     1321 tagacgtcct cgcggcgagc cgactcgacg tcgacatcga tgtcggggag ctcgtcgcgg
     1381 agcgggttca gaaaccgctc gaacacgaga tcgtggcgga cggcgtcgac gtcggagatg
     1441 ccggtgaggt agcagacgag tgacccggcc gccgagcccc ggcaggcgca gcggatcccc
     1501 atggccttga tgtccgcgac gatgtcggcg acc
//
LOCUS       DATBRK010000172         6337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1036935, whole genome shotgun sequence.
ACCESSION   DATBRK010000172 DATBRK010000000
VERSION     DATBRK010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036935"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /gene="cobT"
                     /locus_tag="VIH70_05125"
     CDS             complement(<1..1068)
                     /gene="cobT"
                     /locus_tag="VIH70_05125"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEY8115776.1"
                     /translation="MNRGDLLERAIASISPPYHDVAAGTARLLDAKTKPPRSLGRLEE
                     LACRLAAIRGRVPEGPLRPAIVVAAADHGVAARGVSAYPSEVTAQMLANFASGGAAIC
                     VLAREAGAGLVIVDAGVIGGVAAVDGVRSEVVDGVRGTDDFTIGPAMPRSSAIGMIER
                     GIALAEELAADGVGILGLGEMGIANTTSASALTAALLPADPSLVCGPGTGIDDECLAR
                     KVDAVRRGLAANGLPRRDADPADVLSAVGGLEISFLVGVTLGAAARGIPLLLDGFVSS
                     VAGLVATGFRESATASLIAATRSPEPGHAPVLQRLGLEPLLDLGLRLGEGTGAALALP
                     LVRSAIAILTDMATFDAAGVDG"
     gene            complement(1065..1592)
                     /gene="cobU"
                     /locus_tag="VIH70_05130"
     CDS             complement(1065..1592)
                     /gene="cobU"
                     /locus_tag="VIH70_05130"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEY8115777.1"
                     /translation="MPYTLLIGGARSGKSSLAVRLASAVDGRVVVVATAEARDDDMAE
                     RIRAHRASRPDGWETVEAPIELLDAVDGASKDACVVLDCLTLWVSNAIEAGASGEEID
                     AEARAVTSILAGRRASSVVVSNEVGLGIVPANALARTYRDVLGRVNASFAARASRSLF
                     VVAGRGLPLREVTPT"
     gene            complement(1592..2182)
                     /gene="cobO"
                     /locus_tag="VIH70_05135"
     CDS             complement(1592..2182)
                     /gene="cobO"
                     /locus_tag="VIH70_05135"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY8115778.1"
                     /translation="MIDDEPVEPSSHEPPTDRPARATQHVPSRVLVHTGDGKGKSSAA
                     MGVMLRAVSAGWKVAVIQFVKSGRWRTGEEATGRALGVDWWSLGDGFTWDSTDMDRTE
                     AIAREAWLAAAELIRAGEHRLVVLDEITYPMNWGWIDTAEVVETIRSRPHDVSVVATG
                     REAPAELREVADTVTEMVSAKHAFDEGIPAMRGIEF"
     gene            complement(2179..2946)
                     /locus_tag="VIH70_05140"
     CDS             complement(2179..2946)
                     /locus_tag="VIH70_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013021516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8115779.1"
                     /translation="MRDLSVELGRRRIVSGVHMDVPEGGWVCVIGPNGAGKTTLLHAI
                     AGLLPRSGSVRLWGRPIGELGRRERARTIALVPQLPVIPEGFTVEQYALLGRTPHLGL
                     LASEGPRDLEAVASALERLDLGWAATRRLDTLSGGELRRAVMARAIAQEASLLLLDEP
                     TTGLDIAQQLRVLELVEELRTADGLTVISTMHDLTLAGRFAHRFALLSEGHVVAEGAR
                     AEVLLPHVIAEHYGARVGVIDDGTLGWAVVPLTGPER"
     gene            complement(2985..4025)
                     /locus_tag="VIH70_05145"
     CDS             complement(2985..4025)
                     /locus_tag="VIH70_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY8115780.1"
                     /translation="MSGPDAADRRVSAGTRATVIGIAVLVVAVLVGLLVGPVRIGAAN
                     VMRWFFSFGDAPPGMSDQQALILADLRLPRVIVAGLVGASLAASGAAYQAVFRNPLAD
                     PYLLGAAAGAGLGATIAVVYGPSGALELTVPVAAFVGALVGVGLAYGLGRSTLGGRST
                     TSLVLAGVAVASFLTAVQTFLQQQRSETLRQVYTWILGRLGTTGWDDVRLALPSVFLA
                     LATMWAVRRLLDVIEVGDVEAQSLGLHVARLRLVVVIAASLATAAAVAVAGLIGFVGL
                     IIPHTVRLMVGGSYRSVLPLSVLFGASFLILVDTLARTVVAPAEIPIGVITAFIGAPF
                     FIAVLRRNRRTA"
     gene            complement(4022..4975)
                     /locus_tag="VIH70_05150"
     CDS             complement(4022..4975)
                     /locus_tag="VIH70_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8115781.1"
                     /translation="MGRRLSFVVAFVLALSVAACGSYGGSDDAPAGNGASPSSEPSSF
                     PVAVEGTSGQVTIDERPERIVSLSPTATEDLFAIGAGEQVIAVDDQSNFPPEAPATEL
                     SGFEPNVEAIAGFEPDLVVFATEPGDLGSSLEGLGITALQLDAAPTLEVAYEQMEQLG
                     LATGHADQARALVDDMRSEIEGIVGAADPASGTSFYYELDDTFYSVTSKTFIGQLFEL
                     LGLENIADEVGGGSGGYPQLSAEYILASDPDLIFLADTKCCGQSLKTVSQRPGWHELS
                     AVTRGDVVPLDDDVASRWGPRIVDLVRRISEAVDSAGANAA"
     regulatory      complement(5029..5169)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            complement(5224..>6337)
                     /locus_tag="VIH70_05155"
     CDS             complement(5224..>6337)
                     /locus_tag="VIH70_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8115782.1"
                     /translation="VSARIHPEFVVAGVDAESLSGAELVRRLRAVSPETKVICLADAS
                     PLVAAEMLQAGAAAFVSRDEGAEGVFRAMRAVHAGPVALSPDVAGDVATRLLDQASRV
                     EKLETTIAEISEQLQSMTTAKADFLANVSHELRTPVTVAKGIAYVLRNRGIPEGEEDQ
                     FIGHLEASLEKLSMLIEEMLIVADLDRGTLLLELTQVDLAPLLRQVADESARQFPAVT
                     IEREIHESLPASADPMRFIEIVRQLLDNACRYSPEDQPVLLKARPMDEGVVVSVTDHG
                     EGMARQTASRAFEEPFSTGEDTLRKERAGAGVGLHLARQLVVQHGGILWADPLPSGGT
                     RVSFVIPVHEGDRVGRPEDLGSDPLDELHSLSEPNP"
BASE COUNT          956 a         2347 c         2204 g          830 t
ORIGIN      
        1 tccgtcgacg ccggcggcgt cgaacgtggc catgtcggtc aggatcgcga tcgccgagcg
       61 cacgagcggg agcgcgagcg ccgcgccggt gccctcgccc aggcgcagcc cgaggtcgag
      121 caggggttcg agcccgagcc tctggagcac cggcgcatga ccgggttcgg gcgagcgcgt
      181 ggccgcgatg agcgaggcgg tggccgattc ccggaacccg gtcgcgacca gcccggcgac
      241 actcgagacg aacccatcga ggaggagggg gatcccccgc gccgcggcgc cgagggtcac
      301 gccgaccagg aacgagatct cgaggccgcc caccgccgaa agcacgtcgg ccggatcggc
      361 gtcccggcgc gggagcccgt tcgccgcgag cccccggcga acggcgtcga ccttcctcgc
      421 gaggcactcg tcgtcgatcc cggttccggg gccgcacacg agcgatgggt cggccgggag
      481 cagcgccgcg gtcagggcgc tcgccgacgt cgtgttcgcg atccccatct cgccgaggcc
      541 gaggatcccg acgccgtcgg ccgcgagctc ctctgccagg gcgatcccgc gctcgatcat
      601 gccgatcgcg ctcgaccgtg gcatcgcggg accgatcgtg aagtcgtcgg tgccccggac
      661 gccgtccacg acctccgagc gaacaccgtc gacggccgcg acgccgccga tcacgccggc
      721 gtccacgatg acgagcccgg cccccgcctc gcgggcgagc acgcagatcg cggcacctcc
      781 gctcgcgaag ttcgcgagca tctgcgcggt gacctcgctc gggtacgcgc tcacgccccg
      841 agccgcgacc ccgtggtccg ccgccgcgac gacgatggcc gggcgcaggg gcccctccgg
      901 cacgcggccg cggatggccg ccagccggca cgcgagctcc tcgagtcgtc cgagactgcg
      961 gggcggcttg gtcttcgcgt cgagcagccg cgcggtcccc gccgcgacgt cgtgataggg
     1021 aggggagatg gatgcgatcg cgcgttcgag gagatcgccg cgattcacgt cggcgtcacc
     1081 tcccggagcg gcaggccgcg gccggcgacc acgaagagcg atcgggacgc ccgggcggcg
     1141 aacgacgcgt tcacgcgccc gagcacgtct cggtaggtcc gagcaagggc gttcgccggc
     1201 acgatgccga gtccaacctc gttgctcacg accacggacg aggcgcggcg cccggccagg
     1261 atcgaggtca ccgcccgagc ctcggcgtcg atctcctccc cggaggcgcc ggcctcgatc
     1321 gcgttggaca cccagagcgt gaggcagtcg aggaccacgc atgcgtcctt gcttgcgccg
     1381 tcgaccgcgt cgagcagctc gatcggcgcc tccacggtct cccagccatc cgggcgagat
     1441 gcgcggtgag cgcggatccg ttcggccatg tcgtcgtcgc gagcctcggc ggtggcgacg
     1501 accaccaccc gcccgtcgac cgccgaggcc aggcggacgg cgagcgagga cttcccgctg
     1561 cgcgcccccc cgatcagcaa cgtgtacggc atcagaactc gatcccgcgc atcgcgggta
     1621 tgccctcgtc gaaggcatgc ttcgcgctca ccatctcggt gacggtgtcg gcgacctcgc
     1681 gcagctcggc gggcgcctcc cgcccggtcg cgacgacgct gacgtcgtgg gggcgagatc
     1741 ggatggtctc gacgacctcg gccgtgtcga tccaacccca gttcatcggg taggtgatct
     1801 cgtccaggac gaccagccgg tgctcgcccg cccggatgag ctcggccgcg gcgagccacg
     1861 cctcgcgcgc gatggcctcc gtccggtcca tgtccgtgga gtcccacgtg aagccgtcgc
     1921 cgagcgacca ccagtcgacg ccgagggccc gaccggtggc ctcctcgccc gtgcgccacc
     1981 ttcccgactt cacgaactgg atcaccgcca ccttccagcc ggccgacacc gcacggagca
     2041 tcacgcccat cgcggcggat gatttgccct tgccgtcgcc ggtgtgcacg agcactcggg
     2101 acgggacgtg ttgggtcgcg cgcgcgggcc gatcggtcgg tggctcgtgg ctcgacggtt
     2161 ccacgggctc gtcgtcgatc atcgctccgg ccccgtgagc ggcacgaccg cccacccgag
     2221 cgttccgtcg tcgatcacgc cgacccgtgc accgtagtgc tcggcgatca cgtgggggag
     2281 caggacctcg gctcgtgcgc cctcggccac cacgtgtccc tccgatagca gcgcgaaccg
     2341 gtgcgcgaac ctgcccgcga gggtgaggtc gtgcatcgtc gagatcacgg tgagcccgtc
     2401 ggccgtccgg agctcctcga cgagctcgag cacgcggagt tgctgggcga tgtccaggcc
     2461 cgtggtcggc tcgtcgagca ggaggagcga cgcctcctgc gcgatcgcgc gcgccatgac
     2521 cgcccgtcga agctcgccgc ccgacagcgt gtcgagcctc ctcgtggcgg cccagccgag
     2581 atcgagccgt tcgagcgccg acgccaccgc ctcgaggtcc cgaggtccct ccgaggccag
     2641 caggccgagg tgcggcgtgc gcccgagcag cgcgtactgc tccacggtga agccctcggg
     2701 gatcaccggc agctgcggca ccagcgcgat cgtgcgggcg cgctcacgcc tgccgagttc
     2761 cccgatcgga cgtccccaaa gccgcaccga acccgaacgt ggcagcagcc cggcgatcgc
     2821 atgcaggagc gtggtcttgc ccgccccgtt gggtccgatc acgcacaccc acccgccctc
     2881 ggggacgtcc atgtggacgc cggagacgat ccgacgcctg ccgagctcga cggagaggtc
     2941 ccgcatctcg agcgcggtca ccacgcccgc ccgctcgtcc aacctcacgc cgtcctccgg
     3001 ttccgtcgga gcaccgcgat gaagaacggt gccccgatga acgccgtgat gacgccgatc
     3061 gggatctcgg ccggtgccac gacggtccgg gcgagcgtgt cgacgaggat gaggaacgag
     3121 gcgccgaaca gcaccgagag cggcagcacg gatcggtagc tgccgccgac catcagccgg
     3181 accgtgtgcg ggatgatcag cccgacgaac ccgatcaacc cggcgacggc gaccgcggcc
     3241 gcggttgcga gcgacgcggc gatcaccacg accagtcgca gccgggccac gtgcaggccg
     3301 aggctctgcg cctcgacgtc gccaacctcg atcacgtcga gcaagcggcg caccgcccac
     3361 atcgtcgcga gcgcgaggaa gaccgagggc agcgccaggc gcacgtcgtc ccacccggtc
     3421 gtcccgagcc gccccaggat ccaggtgtag acctggcgga gcgtctccga acgttgctgt
     3481 tgcaggaagg tctgcaccgc ggtcaggaac gaggcgacgg cgacgcctgc caggacgagg
     3541 ctcgtggtcg agcgcccgcc cagggtcgag cgccccagcc cataggccag gccgacgccg
     3601 acgagcgccc cgacgaacgc cgcgacaggg accgtgagct cgagcgcgcc cgacggcccg
     3661 tagacgacgg cgatcgtcgc gccgagcccg gcgccggcgg ccgcccccag caggtagggg
     3721 tcggcgagcg ggttgcggaa caccgcctgg tacgcggcgc ccgaggccgc gagcgacgcg
     3781 ccgacgagcc cggcgacgat caccctcggc agccggaggt cggccaggat caacgcctgc
     3841 tgatcggaca tgcccggcgg ggcgtcgccg aacgagaaga accaccgcat gacgttcgcc
     3901 gcgccgatcc gaaccggccc gaccagcaac ccgacgagaa cggccacgac aaggacggca
     3961 atgccgatga cggtcgcccg ggtcccggcg ctcactcggc gatcggccgc gtcggggccg
     4021 ctcacgcggc gttcgccccc gcggagtcca cggcctccga gatccgccga acgaggtcga
     4081 cgatcctcgg cccccagcgc gaggcgacgt cgtcgtccag cggcacgacg tcgccccggg
     4141 tcacggccga gagctcgtgc cagccggggc gctgcgagac cgtcttaagc gactggccgc
     4201 agcacttcgt gtcggccagg aagatcaggt cggggtcgga cgccaggatg tactcggcag
     4261 acagctgggg gtacccgccg ctccccccgc cgacctcgtc ggcgatgttc tcgagcccga
     4321 gcagctcgaa cagctgcccg atgaaggtct tcgacgtgac cgagtagaac gtgtcgtcga
     4381 gctcgtagta gaagctggtc cccgacgccg gatcggcggc gcccacgatc ccttcgatct
     4441 ccgaacgcat gtcgtcgacc agcgcccgcg cctggtccgc gtggccggtc gcgaggccga
     4501 gctgttccat ctgctcgtac gcgacctcga gcgtcggagc ggcgtcgagc tgcagcgccg
     4561 tgatccccag accctcgagc gacgacccga gatccccggg ctcggtggcg aacacgacga
     4621 ggtcgggttc gaatcccgcg atcgcctcca cgttgggttc gaacccggac agctcggtcg
     4681 caggtgcctc cggcgggaag ttggactggt cgtccacggc gatcacctgc tcgccggccc
     4741 cgatcgcgaa caggtcctcg gttgccgtcg gcgagagcga cacgatccgt tccggccgtt
     4801 cgtcgatcgt gacctgcccg ctcgtcccct cgaccgcgac ggggaacgac gatggctccg
     4861 aggacggcga ggcgccgttg ccggccggcg cgtcgtccga gccaccgtag gaaccacacg
     4921 ccgcgaccga cagcgcgagc acgaacgcaa cgacgaagct gagacgcctg cccatccgcc
     4981 ctcctcccga tccgaaggag ctgcaatggg cctcgatctc gccgcccaca aaccgccctt
     5041 ccttcgagga ccgtcgtttg cgcgacgagg gaggcgaccc gactcgtccc ccgaaggggc
     5101 ttcacggttg cgggacagtg ccggattcaa accggtcttc gctgcacctc gtcgcacccg
     5161 ggctcccgtg cccgggcacc tgtgttgtac caccgcgctc ggacggagcg caacgaaacg
     5221 cccctacggg ttcggctcgg acagcgagtg gagctcgtcg agcggatcgg acccgaggtc
     5281 ctcgggacgg ccaacgcgat cgccctcgtg gaccgggatc acgaacgaga cgcgggtgcc
     5341 gccggagggg agcgggtctg cccacaggat cccgccgtgc tggaccacga gctgccgagc
     5401 caggtgcagt cccacgcccg ctcctgcgcg ctccttgcgc agggtgtcct cgcccgtcga
     5461 gaacggctcc tcgaatgccc tcgacgctgt ctgccgcgcc atcccctcgc cgtgatcggt
     5521 caccgagacc accacgccct cgtccatcgg acgggccttg agcagcaccg gctggtcctc
     5581 gggggagtag cggcacgcgt tgtcgaggag ctgacgcacg atctcgatga accgcatcgg
     5641 atcggccgac gccgggaggc tctcgtggat ctcgcgttcg atcgtgaccg cggggaactg
     5701 gcgcgcgctc tcgtccgcca cctgccgcag gagcggcgcc aggtcgacct gggtgagctc
     5761 gagcaagagc gttccgcggt ccaggtcggc gacgatcagc atctcctcga tcagcatcga
     5821 gagcttctcg agcgaggcct cgagatggcc gatgaactgg tcctcctccc cctcggggat
     5881 cccgcggttc ctcaggacgt acgcgatccc cttggccacg gtgaccggcg tccggagctc
     5941 gtgtgagacg ttggcgagga agtcggcctt ggcggtggtc atcgactgga gctgctcgct
     6001 gatctcggcg atcgtcgtct cgagcttctc gacccggctc gcctggtcga gcaagcgggt
     6061 ggcgacgtcg ccggcgacat cggggctgag cgcgacgggt cctgcgtgca cggcgcgcat
     6121 cgctctgaac acgccctcgg ccccttcgtc cctggagacg aaggcggcgg cgccggcttg
     6181 gagcatctcg gcggctacca gcggcgaggc gtccgccaag cagatcacct tggtctcggg
     6241 gctgacggcc cgcaggcggc ggacgagctc ggccccggac aggctctcgg catcgacgcc
     6301 ggcgacgacg aactccgggt ggatgcgcgc ggagacc
//
LOCUS       DATBRK010000173         6631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1901923, whole genome shotgun sequence.
ACCESSION   DATBRK010000173 DATBRK010000000
VERSION     DATBRK010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901923"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VIH70_05160"
     CDS             complement(<1..189)
                     /locus_tag="VIH70_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEY8115783.1"
                     /translation="MPGALDFLKQRLAELDQQGLLLQPRTLEGPTGARARFDGRDVIN
                     LASNNYLGLASHPRMNAAA"
     gene            250..726
                     /locus_tag="VIH70_05165"
     CDS             250..726
                     /locus_tag="VIH70_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115784.1"
                     /translation="MGDIELDAFLADSVASVQGKLYALGAGWNRIAVALFPARHDRVG
                     IGLLVRIPSGSVVEPRRFELRLLGPSGQDLSLGAGPEGPVNRIGGEFTAGGAEEQIVP
                     IALNLNGLPLPGPGDYRVVISMQGDDVKVLPFTVQALAAQPRQEPPATTTGTAGYL"
     gene            complement(717..1778)
                     /gene="tdh"
                     /locus_tag="VIH70_05170"
     CDS             complement(717..1778)
                     /gene="tdh"
                     /locus_tag="VIH70_05170"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY8115785.1"
                     /translation="MRALRKIEPRPGAELVDVPVPEPGEGEVLVRVHAASICGTDLHI
                     FDWNEWAQGRVTRVPMTFGHEVAGTVETVGPEVHHVQQGAFVAVETHIACGVCSTCRT
                     GRAHICRNLQILGVDTDGAFAEYVVVPAENAWIVGEGIQPDHASVMEPFGNAVHATFG
                     TEGGEDLLTNPVAVIGCGPIGLFAVGIARALGAWKVIAIEPNDERRSLAAKMGADLLI
                     DPKADDPVEAVLDATDGNGAEVVLEMSGNATAIDQGTRLLARGGRMSLLGLPDGPVTL
                     DLNDQVIFKEARILGITGRQMFRTWQQTTTLLSTGRVDISPVITHRFPLDRYEEAFET
                     AGSGHAAKVIVFPSGNDQR"
     gene            1925..2806
                     /locus_tag="VIH70_05175"
     CDS             1925..2806
                     /locus_tag="VIH70_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115786.1"
                     /translation="MRVGRIGVAVAAVALLASSCGGVGNDEPGSGGGTGATGIAHPTG
                     SDQLILRMAYEGGFVPYEYTLGGIPFWSLFGDGTLIVPGPQIEIYPGPALPNLTATPV
                     SEDGIQAILEAARDAGLLDGDANYGNECIADAANTVFTTTADGTTTVVSAYALDVGDP
                     AGTCGNDKDADARAKLAAFQAKLTDLRSWLPNGSVGTERPYDPTEMRVYVLPYRGDAE
                     LPQDPIEWPLERPLDSFGGPVENASSDTRCGVVAGEALTTLLAAAKDANALTPWTSGG
                     TKYQLILRPLLPDEHTC"
     gene            complement(2983..3591)
                     /locus_tag="VIH70_05180"
     CDS             complement(2983..3591)
                     /locus_tag="VIH70_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8115787.1"
                     /translation="MFLLVIVVSLAGLRWIRSEHLEALRSVPLFRSLSKHQLMRILRA
                     ARAVEFQPNAEIIREGEAGKGLYTVTDGSAKVLVGGNRLATLGSGSYFGEMAVIDGGP
                     RTATIVAATRVSTLELTPSAFLRILDSEPALARALSAELCGRLHDAGGYSGDCGDDAP
                     VDRARLVELCQRLRRTEHPEWTQATPSRRRWLGLSSLFARGS"
     gene            4359..4850
                     /locus_tag="VIH70_05185"
     CDS             4359..4850
                     /locus_tag="VIH70_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115788.1"
                     /translation="MVGSFQRGARVVALAAVVVFVASACSNDSTPTAPIYNVFGQQFR
                     FHGMPKSIPSGNIQINFSNKESFPITHEMIVKALPKGDTAQDIIDSAKVPGCEGGAEC
                     ETQYLAFGEIAGVDTGATLSNVFDLPAGEYFFACWETGTQSGGENGPPHASKGMVFTF
                     TVT"
     gene            4874..5896
                     /gene="coxB"
                     /locus_tag="VIH70_05190"
     CDS             4874..5896
                     /gene="coxB"
                     /locus_tag="VIH70_05190"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY8115789.1"
                     /translation="MRRIRARLARLGLAPVLLLLTNCAKQGISPQGQDVHRLYVIILI
                     LAAPVFIAVEAALIWCIVRYRKRSDDPAPQSVGGSRSLGVFFVIPAVIVALLFPLGET
                     TLLRIDRTEPPQVQIRVEGFQWQWTFLYLQEGIFVSGKTLVKPAEMVVPVDEPVRITL
                     TSRDVIHSFFVPDLLFKRDAIPGRTSTFEFTPTELGTFPAQCAEFCGLWHSKMTFDVK
                     VVSSLDYQAWIKQQRKAAASITCKPNGTTLSLVAHNISWNEFCLAVPANTPFTVNINN
                     QDAGIQHNFSIYDSFFQKTTYFTGPKITGPATVSLNPKPLPPGRYYFQCDVHGPAMSG
                     AFVVKG"
     gene            5924..>6631
                     /locus_tag="VIH70_05195"
     CDS             5924..>6631
                     /locus_tag="VIH70_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8115790.1"
                     /translation="MAVTEAPTTKLAPEPERGRLLDWLKTTDHKKIGILYLINSFAFF
                     AVGGVFALLMRTELARPGTQIFAPHAYNQLFTLHGTLMIFLVIFPLLAGFGNYFVPLQ
                     IGALDMAFPRINALSFWLLPVGGLTIVSGFLARGGAAAAGWTGYAPLSEQLGTGQDLW
                     ILGLIIVGTASILGGINFIVTILRMRAPGMTMMRMPVFTWSILATSLLVILAAPVLTA
                     GLIMVFADRQIGTVFFDP"
BASE COUNT         1105 a         2246 c         2161 g         1119 t
ORIGIN      
        1 ggccgcggcg ttcatccggg ggtggctcgc cagcccgagg tagttgttcg aggcgaggtt
       61 gatcacgtcc cgcccgtcga accgagcccg ggcgcccgtg ggtccctcga gggtcctcgg
      121 ctgcagcagg agtccctgct gatcgagctc ggcgagccgc tgcttcagga agtcgagcgc
      181 acccggcatc ggaacctcac gctaccgcat acgctatgtc tcccatccgg ccgaccgagg
      241 ggaggcatcg tgggcgacat cgagctcgac gcgttcctcg ccgactccgt cgcgtcggtg
      301 cagggcaagc tctacgcgct cggggcgggg tggaaccgca tcgccgtcgc gctgttcccg
      361 gcccgtcacg accgggtggg gatcgggctc ctggtccgaa tcccgtccgg ttcggtcgtc
      421 gagccgcgcc ggttcgagct tcggctgctc ggcccgtcgg gacaggacct ctcgctcggc
      481 gccggtcccg agggcccggt caatcgcatc ggcggcgagt tcaccgcggg cggcgcggag
      541 gagcagatcg tgccgatcgc cctgaacctg aacgggctgc cgctccccgg cccgggcgac
      601 taccgcgtcg tgatctcgat gcagggggac gacgtgaagg tgctcccgtt caccgttcag
      661 gcgctcgcag cccagcctcg acaggagccg ccggcaacca cgaccgggac ggccggctac
      721 ctctgatcgt tcccgctcgg gaacacgatc accttcgcgg cgtgacccga gccggccgtc
      781 tcgaacgcct cctcgtaccg atccagcggg aaccggtgcg tgatcacggg agagatgtcg
      841 acccgccccg tggagagcag cgtagtcgtc tgttgccacg tccggaacat ctggcgcccg
      901 gtgatcccca ggatccgcgc ctccttgaag atcacctggt cgttcaggtc gagcgtcacc
      961 gggccgtccg ggagcccgag caacgacatg cgcccacccc gcgccagcag gcgggtgccc
     1021 tgatcgatcg ccgtcgcgtt gcccgacatc tcgagcacga cctccgcacc gttgccgtcc
     1081 gtcgcatcga gcaccgcctc gacggggtcg tccgccttcg ggtcgatcaa caggtccgca
     1141 cccatcttcg ccgcgagcga ccggcgttcg tcgttgggct cgatcgcgat caccttccag
     1201 gcgcccagcg cgcgggcgat ccccacggcg aacaggccga tcgggccgca gccgatgacc
     1261 gcgaccgggt tcgtgagcag gtcctcgccg ccctcggtcc cgaacgtcgc gtgcacggcg
     1321 ttcccgaagg gttccatcac cgacgcgtgg tcgggctgga tgccttcacc cacgatccac
     1381 gcgttctccg ccggcacgac gacgtactcc gcgaacgccc cgtcggtgtc cacgcccagg
     1441 atctggaggt tgcggcagat gtgtgctcgt cccgtccggc aggtggagca gacaccgcac
     1501 gcgatgtggg tctcgaccgc cacgaaggcg ccctgctgca cgtggtggac ctccggaccg
     1561 accgtctcca ccgtcccagc cacctcgtga ccgaaggtca tcggcacgcg ggtcacacgg
     1621 ccctgggccc actcgttcca gtcgaagatg tggaggtcgg tcccgcagat ggacgcggcg
     1681 tgcacgcgga cgagcacctc gccctcgccc ggttcgggga ccgggacgtc gacgagctcg
     1741 gcaccggggc gaggttcgat cttgcgaagg gcgcgcatgg tgcccagcgt agctcgaccg
     1801 gacgcatcga ggcgcttgcg gtggaaccat ggggcccgac cgcgccgtcc aagccggact
     1861 cgacggccgg actcgacgga cggccgcgct cgacgaacgg ccagactcga cgatgggagc
     1921 gatcatgcgc gtgggacgga tcggtgtggc ggtcgcggcg gtggccctgc tcgcctcatc
     1981 gtgcggcggg gtggggaacg acgagccggg ctcgggtggc gggacggggg cgaccggcat
     2041 cgcgcacccg acggggtcgg accagctgat cctgcggatg gcctacgagg gcgggttcgt
     2101 cccgtacgag tacaccctcg gcggcatccc cttctggtcg ctgttcggcg acgggacgct
     2161 gatcgtgccg ggtccacaga tcgagatcta tccgggcccg gcgctgccga acctcaccgc
     2221 gaccccggtg tccgaggacg gcatccaggc gatcctggag gccgcgcggg acgccggcct
     2281 gctcgacggc gatgcgaact acggtaacga gtgcatcgcc gacgccgcga acactgtctt
     2341 cacgaccacc gcggacggca ccacgacggt cgtctccgcg tacgcgctcg acgtgggcga
     2401 ccccgccggc acctgcggga acgacaagga cgccgacgcc cgcgcgaagc tcgcggcctt
     2461 ccaggccaag cttaccgacc tgcggagctg gctgccgaac ggttcggtcg gcaccgaacg
     2521 gccctacgac ccgaccgaga tgcgcgtgta cgtcctcccc taccggggag acgccgaact
     2581 gccgcaggat ccgatcgagt ggccgctcga gcgcccactg gactcgttcg gagggcccgt
     2641 cgagaacgca tcgtcggaca cgcgctgcgg cgtggtcgcg ggtgaggccc tcacgacgct
     2701 gctcgcggcg gcgaaggacg cgaacgcgct caccccgtgg accagcggcg ggaccaagta
     2761 tcagctgatc ctccgacccc tgctccccga cgagcacacc tgctagcgct cggttgcgac
     2821 ggggccgagc gcatccgggc caggcgggcc atggcgagcc ctggcgagat gagcggtcat
     2881 ggtgggcttc ggtcacgaag gggcatcatc cgaggggaag gacctccgct ttgatctgtt
     2941 gcgggtcccc gcggggaggt ccccggggcc agttcagagg acttaggatc cgcgcgcgaa
     3001 gaggctactg agcccgagcc agcgtctccg ggacggcgtt gcctgtgtcc attcggggtg
     3061 ctcggtcctg cgcaatctct ggcaaagctc aacgagcctc gctcggtcga ccggtgcgtc
     3121 atcgccacag tcccccgagt aacccccagc atcgtgaaga cgaccgcaca gctctgccga
     3181 cagagctcgg gcgagcgctg gctcgctgtc caagatgcga aggaacgctg aaggagtgag
     3241 ttcgagtgtg gacacccggg ttgccgcaac gatggttgcc gtccttggac cgccgtcgat
     3301 caccgccatc tcaccgaagt acgaccccga tcccagggtg gccaatcggt tccctccgac
     3361 gagtaccttg gcgctcccat ctgtgacggt gtagagcccc ttcccggctt cgccctcccg
     3421 aatgatctcg gcgttgggct ggaactcgac ggcgcgggcc gctcgcagga tgcgcatcag
     3481 ttggtgcttg gacaggcttc tgaagagggg gacggagcgc agtgcctcca ggtgttccga
     3541 cctgatccaa cgaagccccg ccaagctgac aacgatgacg aggaggaaca tcagagcggg
     3601 aaccaaccag gacgccgcgg cactggctgc gaggaacaaa gccttcagcc cccgcaacca
     3661 agtcgtcaca tccgtcattc caacgacggc cgagaagatc cgggagagcc gtcttcctcc
     3721 gaacgaagga cgatagcttc gtgaggggag cggctgctag ggtgatgacg acattgtcac
     3781 ggagtgctct ctcgctgtcc ttgggcaggc ggatggaatt ggcgcccgct ccactctgac
     3841 ggcggccctg agctctcccg agtgactcag acctcgtgct cgctacctgc tcggctgcgc
     3901 tgtccggatc cgagaagctc ttgcgcaact gcgcagagcg actccggtca agatggttca
     3961 tcagtagccc actcgatggc ccgacgagca aggagcgggc cgcgttcggc ttgaccgtcc
     4021 gacggtggag aaaatacgct tcgccttggc cctgagaggc gggcggaaga acaccgtcgt
     4081 cggacaacgt ttctcggggc cgcctcttcg ttggtcggac cctgcagctg accaggacct
     4141 cacgagtgga cctcgccagt catctcgtcc cagagaccgt ccgcccaccg cctgctgaga
     4201 tacatgacaa ccgtgcaatg tggcaagtcc gtgcgccttg tatcggcgcc tccagccgag
     4261 atcgtcgtga cagtcctgtc atccatcttg cctccgagcc cgggaccgct cgaaagtagg
     4321 tcttcacggg tgcccgctga ctgggacgga ggggtggcat ggtcggatcg ttccaacgtg
     4381 gggctagggt ggtcgcgctg gcggccgtcg tggtgttcgt agcgtcagcg tgctccaacg
     4441 acagcacgcc gaccgcgccg atctacaacg tcttcgggca gcaattcagg ttccacggca
     4501 tgcccaagtc gatcccctcg ggaaacatcc agatcaactt ctcgaacaag gagtcgttcc
     4561 cgatcacgca cgagatgatc gtgaaggcgc tccctaaggg ggatacggcg caagacatca
     4621 tcgacagtgc gaaggttccg gggtgcgagg gcggcgccga gtgcgagacc caatacctgg
     4681 ccttcggtga gatcgccggt gtggacacgg gggcgaccct ctcgaacgtg ttcgatctac
     4741 cggcagggga gtacttcttc gcatgctggg agacgggaac ccagagtgga ggggagaacg
     4801 gtccgcccca cgcttcgaag ggcatggtgt tcacgttcac ggtgacctga gagaggcgaa
     4861 gcggtcaacc cggatgcgca ggatccgcgc gcgactagct cgtctcgggc tcgcgccggt
     4921 cttgctgttg ctgacgaact gcgcgaagca ggggatttcg ccccagggtc aggatgttca
     4981 tcggctgtac gtgatcatcc tgatcttggc cgcgccggtc ttcatcgcgg tcgaagcggc
     5041 cctgatctgg tgcatcgtgc ggtatcgaaa acgctcggac gatcctgcgc cacagtccgt
     5101 cggcgggagt cgctcgctgg gcgtgttctt cgtgatcccc gccgtgatcg ttgctctcct
     5161 gttccccctc ggcgagacga ccctcctgcg gatcgaccga accgaaccgc cacaggtgca
     5221 gatccgggtc gagggcttcc agtggcaatg gacgttcctc tacctgcagg agggcatctt
     5281 cgtcagcggc aagaccctcg tgaaacctgc ggagatggtc gtgcccgtcg acgaaccggt
     5341 ccggattacg ctgacgtccc gcgacgtgat ccattccttc ttcgtgccgg atcttctctt
     5401 caagcgtgac gcgatccccg gcaggaccag cacgttcgaa ttcacaccga cagaactcgg
     5461 gaccttcccc gcgcagtgcg ccgagttctg cggcctctgg cattcgaaga tgacgtttga
     5521 tgtgaaggtg gtgagttccc tcgactacca agcctggatc aagcagcagc gcaaggcagc
     5581 cgcaagcatc acgtgcaagc ccaatgggac aaccctgtcg ctcgttgcac acaacatctc
     5641 gtggaatgaa ttctgcttgg ccgtccccgc gaacacaccc ttcaccgtca acatcaacaa
     5701 ccaggacgcc ggcatccagc acaacttctc gatctacgac agcttcttcc agaagaccac
     5761 gtatttcacg ggaccaaaga tcaccggacc ggcaaccgtc tcgctcaacc ccaagccgtt
     5821 acctcccggc cgctactact tccagtgcga cgtccacggt ccggcgatgt cgggggcgtt
     5881 cgtcgtcaag ggatgaggaa gagatgagct cgaggggaac gacgtggccg taaccgaggc
     5941 tccaaccacc aagctcgcgc cggagccgga gcgaggccgg ctgctcgact ggttgaagac
     6001 gaccgatcac aagaagatcg ggatcctcta tctgatcaac agcttcgcgt tcttcgcggt
     6061 cggcggtgtg ttcgccctcc tgatgcgcac ggaactcgcc cggcccggaa cgcagatctt
     6121 cgctccccac gcgtacaacc agctcttcac gctgcacggg acgctcatga tcttcctcgt
     6181 gatctttccc ctcctcgcgg ggttcggtaa ctacttcgtt ccgctgcaga taggtgcgct
     6241 cgacatggcg ttcccacgca tcaacgccct gtccttctgg ctgctgccgg tgggcgggct
     6301 gaccatcgtg tcgggattct tggcacgggg cggtgccgcc gccgcgggat ggaccggtta
     6361 cgccccgctg tccgagcagc tgggcactgg ccaggacctg tggatcctcg ggctcatcat
     6421 cgtcggaacg gcctcgatcc tcggcgggat caacttcatc gtgacgatcc ttcgcatgcg
     6481 agcgcccgga atgacgatga tgcggatgcc ggtgttcacc tggagcatcc tcgcgacctc
     6541 gctcctcgtg atcctcgctg ccccggtgct cacggccggg ctgatcatgg tgttcgccga
     6601 tcggcagatc gggaccgtgt tcttcgatcc c
//
LOCUS       DATBRK010000174         3304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2192344, whole genome shotgun sequence.
ACCESSION   DATBRK010000174 DATBRK010000000
VERSION     DATBRK010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192344"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VIH70_05200"
     CDS             <1..726
                     /locus_tag="VIH70_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115791.1"
                     /translation="VGEGSVWVTAPELRPDGSRGNLVRIDPDTNEIAATIHIGGPIQY
                     LAVGGGSVWVTMPDAGTSTVFRVDPGSDRLIGSIPVAEASGPVVIGEGSVWVTHPWVG
                     GSVTEIDPTTDQVVGTFDVPSVQAVGDGSLWGSAGDSVLRIDPDSGSVQAAIPIPRAQ
                     TVAFDGSTVWVLVSPRSSDPELFYPIAGTAGVVRVDPATDRVLGEPVALEDLQPIAMA
                     ADDQGAWVADYYDGILSRVEVGG"
     gene            832..1710
                     /locus_tag="VIH70_05205"
     CDS             832..1710
                     /locus_tag="VIH70_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY8115792.1"
                     /translation="MLRRIAFVVSALVLVPISESVALAQGRLCFAREATITGTEGADV
                     LHGTPENDVILGLGGDDIVRGGKGNDRMCGGDGADSIRGSPGSDRIDGNAGADTIAAD
                     GGKDLANGGSGNDSIGGGSGRDRLVGQGGADVLDGGGGRDWVGYFLARSEVRANLSTH
                     RASGEGLDTLVHVEDLEGSPYDDVLVGDARGNDIDGGNGSDALLGSDGGDRLLGGRGD
                     DRLRGGVGADVLDDSLRVAQGVFPDTGADLLSGGPGDDRLRGGDEVRANDTLHGGAGH
                     DACSADPGDRISGCRP"
     gene            complement(1786..2076)
                     /locus_tag="VIH70_05210"
     CDS             complement(1786..2076)
                     /locus_tag="VIH70_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115793.1"
                     /translation="MLQVTDAAVSVFKKVLEQEERGGGGIRLVPNPQPDGRTSVGVEP
                     IREAGPNDEPTDARGLTVVVARDLAPELENSVLDAEETATGADLLVRPQQKT"
     gene            2280..>3304
                     /locus_tag="VIH70_05215"
     CDS             2280..>3304
                     /locus_tag="VIH70_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEY8115794.1"
                     /translation="MSMLRSALDELRGDDLRSLSDEALAGHLDEIERADRILEFERGR
                     ALAEVERRKVYAVDGHLSAAAWLAHRQGLSRSASEGRVRRARALEAMPEVARAFGEGE
                     VSESAVHVLASAQEAAPEAFARSEPALVEAARSMPFGELRRVTETWRIAADPERALVE
                     EDRRHERRRLDICPTEDGMTGVRAELDAEDGQGLITAIRAQMDAETRSANGPDLRTPT
                     QRRADALGEICRQWLASRERPTIGGERPHVVVTIDLETLIERSGLRSELQDTGQFTPE
                     TARRLACDADVTRVITREGSEPLELGRRTKVVPPGLRRAVAVRDRGCRFPGCGRPPGW
                     CDAHHVR"
BASE COUNT          458 a         1108 c         1216 g          522 t
ORIGIN      
        1 gtgggggagg gttcggtgtg ggtgaccgcg cccgagcttc ggcccgacgg gtcgaggggg
       61 aacctcgtcc gcatcgatcc cgatacgaac gagatcgcgg cgacgatcca catcggcggc
      121 ccgatccagt acctcgccgt cggcggcggt tcggtgtggg tgacgatgcc ggacgcaggg
      181 acgagcacgg tcttccgggt cgaccctgga agcgatcgct tgatcgggag catccccgtg
      241 gccgaggcgt ccggccccgt cgtcatcggc gagggatccg tgtgggtcac ccacccctgg
      301 gtcggcggat ccgtgaccga gatcgacccg acgaccgacc aggtcgtcgg cacgttcgat
      361 gtcccgagcg tgcaagcggt cggcgacggt tcgctgtggg ggtcggcggg cgactccgtg
      421 cttcggatcg atcccgattc ggggagtgtg caggccgcga tcccgatccc tcgggctcag
      481 acggtggcct tcgatggatc gacggtgtgg gtcctggtct cgccccgctc cagtgacccg
      541 gagctcttct atccgatcgc cggcaccgca ggtgtggttc gggtcgatcc cgcgaccgat
      601 cgtgtgctcg gcgagcccgt cgcgctcgag gatctccagc ccatagcgat ggcggcggac
      661 gaccagggtg cgtgggtggc cgactactac gacgggatcc ttagccgggt cgaggtcggc
      721 ggatgatggt tcgccggtcc gctgcgatca ccgttcccgc aaagcctcga ccagccgctc
      781 ggcagacgcc tcgacccatc gggcgagtgg gccgacgtat gctccgacgg catgctgcga
      841 cgcatcgcgt tcgtcgtgtc ggctctcgtc ctggttccga tctccgaatc cgtcgccctg
      901 gcgcaggggc gactctgctt cgctcgcgag gccacgatca ccggaacgga gggggccgac
      961 gtcctgcacg gcacgcccga gaacgacgtg atcctcgggc tgggcgggga cgacatcgtg
     1021 cgaggcggca aggggaacga ccggatgtgc ggcggcgacg gcgccgactc gatccgaggc
     1081 agtcccggat cggatcggat cgacggcaac gcgggcgccg acacgatcgc cgccgacgga
     1141 ggcaaggatc tcgcgaacgg cgggtcgggc aacgactcga tcggcggcgg ctcggggcgg
     1201 gaccgcctgg tcgggcaggg tggtgccgac gtgctggacg gcggcggagg gcgcgattgg
     1261 gtcgggtact tcctcgcacg cagcgaggtc agggcgaacc tctcgacgca ccgcgcctcc
     1321 ggggagggcc tcgacacgct cgtgcacgtc gaggacctcg agggctcccc atacgacgac
     1381 gtcctggttg gtgacgcgcg tggcaacgac atcgacggcg gaaatggatc cgatgcgctg
     1441 ctcgggtccg acggtggcga ccgtctgctc ggcggacgcg gggacgaccg gctccgcggt
     1501 ggcgtgggtg cggacgtgct cgacgattcc ctccgcgtcg cacagggcgt gttcccggac
     1561 accggcgccg atctcctgtc gggtgggccg ggcgacgatc gtctgcgggg cggcgacgag
     1621 gtccgggcga acgacacgct gcatggggga gcgggccacg atgcctgctc ggccgatccc
     1681 ggcgatcgga tctcggggtg ccgtccctag gaccgtgata tccgacccga tcagacgcgt
     1741 cgggcgagcc gtcgcaacta gggtcagccg actgctgggc ctacgctagg tcttctgttg
     1801 ggggcggacc aagaggtcgg cgcccgtcgc ggtctcctcg gcatccagga ccgagttctc
     1861 gagctcgggg gcgaggtccc tcgcgacgac gaccgtgagg ccgcgcgcgt ctgtcggctc
     1921 gtcgttcggt ccggcctctc tgatcggctc gaccccgacg gacgtgcggc cgtccggctg
     1981 aggattcgga acgaggcgga tcccgccgcc gccgcgctcc tcttgctcga gaaccttctt
     2041 gaacactgat acggctgcgt cggtgacctg aagcatcgcg ggcctccttc cgacatctct
     2101 ctagctttac ccccggcgct cgacggcaca cgcggaccgg ccggttctgc gagttgggga
     2161 tccgccggtg gatacggcgt ctgacggcgg cctggcgggc cgtggtttga cgaacttcct
     2221 ttgccgagtg tgaccttgtt cacaatgagt cgaacgtgcg ttcgagtagt gtcgagcaca
     2281 tgagcatgct gcgatcggcg ctcgacgagc tgcgcgggga cgacctccgc tcgttgtcgg
     2341 acgaggcgct cgccggtcac ctggacgaga tcgagcgcgc cgatcgcatc ctcgagttcg
     2401 agcgcggtcg cgcccttgcg gaggtcgaac gccggaaggt ctacgcggtc gacggtcacc
     2461 tgtcggccgc ggcatggttg gcgcaccggc aggggctgtc gcgttcggca tccgagggcc
     2521 gcgtgcgtcg cgctcgtgcg ctcgaagcga tgcccgaggt ggcccgcgcg ttcggggaag
     2581 gtgaggtctc tgaatctgcc gtccacgtgc tcgcatccgc ccaggaggct gctccagagg
     2641 cgttcgcgcg atccgaaccc gcgctcgtcg aggctgcgcg ctcgatgccc ttcggcgagc
     2701 tccgccgggt gaccgagacg tggcggatcg ccgccgatcc cgaacgggcg ctcgtcgagg
     2761 aggatcgtcg ccacgagcgc cggcgcttgg acatctgccc caccgaggac ggcatgacgg
     2821 gcgtgcgggc cgagctggac gcggaggacg gccagggcct catcaccgcg atccgggcgc
     2881 agatggacgc tgagacgcgt tcggcgaacg gaccagacct tcgcacgccc acccagcggc
     2941 gggccgatgc gctcggcgag atctgtcgtc agtggctcgc ctcgcgcgag cgtccgacca
     3001 tcggcggcga acgcccgcac gtcgtggtca cgatcgacct cgagacgttg atcgagcgtt
     3061 ccggactgcg ctcggagctg caggacaccg gccagttcac gcccgaaacc gctcgccgcc
     3121 tcgcctgcga cgccgacgtg acccgggtga tcacgcgaga gggctccgag ccgctcgagc
     3181 tcggccgtcg caccaaggtc gtcccgcccg gcctccgacg cgccgtcgcc gttcgggacc
     3241 gcggatgtcg gttcccgggc tgtggacggc cacccggctg gtgcgatgcc catcacgttc
     3301 gcca
//
LOCUS       DATBRK010000175         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2628450, whole genome shotgun sequence.
ACCESSION   DATBRK010000175 DATBRK010000000
VERSION     DATBRK010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2628450"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VIH70_05220"
     CDS             <1..365
                     /locus_tag="VIH70_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8115795.1"
                     /translation="RPDVFHAGIVGAPVTDMRLYDTHYMERYLGRPQEEPEAYRRSSP
                     LPDAPALSRPMLLIHGLADDNVFVANTLQLSAALFEAGKWHELVLIPNATHLTRSTAV
                     TENVLRIQLDFLKRTLVR"
     gene            complement(367..696)
                     /gene="fdxA"
                     /locus_tag="VIH70_05225"
     CDS             complement(367..696)
                     /gene="fdxA"
                     /locus_tag="VIH70_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012731278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY8115796.1"
                     /translation="MAYVIAEPCIDIKDKACVDECPVDCIYEGPRMLYIQPDECVDCD
                     ACVPVCPVTAIFSDEELPGEWKQFEAVNAEWFSDAVTGLGSPGGAAQAGPQDKDHKVV
                     AEWPAVA"
     gene            complement(919..1221)
                     /locus_tag="VIH70_05230"
     CDS             complement(919..1221)
                     /locus_tag="VIH70_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8115797.1"
                     /translation="MEGEAPTARRLIDAERRVHRILRAQFTDALWDLGTSFAQVEVME
                     LLHAAVQLHPGEIGRRLLITRQSATHLVRQLERASLVETWRLEGGSVGVRLTEIGR"
     gene            1503..>1887
                     /locus_tag="VIH70_05235"
     CDS             1503..>1887
                     /locus_tag="VIH70_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY8115798.1"
                     /translation="MPIVVQKYGGTSVGDTDRIRGVADRIVRAREQGQDVVVVVSAMG
                     ETTDDLLGKASDITADPEPRELDMLLTSGERIAMSLLAIAVNARGCRAASYTGSQAGI
                     ITDTQHGKARIVEIRPQRIRQSLDAG"
BASE COUNT          315 a          679 c          612 g          281 t
ORIGIN      
        1 ggcggccgga cgtcttccac gccgggatcg tcggcgcccc cgtcaccgac atgcggctct
       61 acgacaccca ctacatggag cggtacctgg gacggcctca ggaggagccc gaggcgtacc
      121 ggcggagctc gccgctgccg gacgcccccg ccctgtcgcg gccgatgctc ctgatccacg
      181 gtctggccga cgacaacgtc ttcgtcgcca acacgttgca gctgtcggcg gcgctgttcg
      241 aggcggggaa gtggcacgag ctggtgctga tcccgaacgc cacgcacctc acgcgctcga
      301 cggcggtgac cgagaacgtc ctgcggatcc agctcgactt cctgaagcgg acgctcgttc
      361 gctagctcag gcgacggccg gccactcggc gacgaccttg tggtccttgt cctgcggacc
      421 cgcctgggcc gccccaccgg gtgagcccag cccggtcacg gcgtccgaaa accactcggc
      481 gttgacggcc tcgaactgct tccattcacc ggggagctcc tcgtccgaga agatcgcggt
      541 gacggggcac accggcacgc aggcgtcgca atccacgcac tcgtccggct ggatgtagag
      601 catccgaggc ccctcgtaga tgcagtcgac cgggcactcg tcgacgcacg ccttgtcctt
      661 gatgtcgatg cacggttcgg cgatgacgta tgccatgcgt ggttggttcc ccggtgtcga
      721 ggacccgaaa cgttgcctcg tggcgatggg cgagctggat gcctagaagg gatccctgac
      781 ccaccagggc aggagacggg gtcgaagcgc gtcctcccaa gctcggaggt cattcacgag
      841 cctgctcagg cgatccccgt cgagcctctc gatcaggtcg aacgtcggac ggagggcgtc
      901 gaagcggtgg accatactct accggccgat ctcggtcagg cggacgccga cgctcccgcc
      961 ttcgagccgc caggtctcga ccagcgacgc gcgctcgagc tggcgaacga ggtgggtggc
     1021 cgattgtcgg gtgatgagga gccgacgacc gatctcgccg gggtgcagct gtaccgccgc
     1081 gtgcaacagc tccatgacct cgacctgcgc gaacgacgtt ccgagatccc agagcgcatc
     1141 ggtgaactgg gctcgcagga tgcgatggac gcgccgctcc gcgtcgatca gtcgccgagc
     1201 agtgggagcc tccccctcca ccggaaggtc ttaccgcgag gcgccgacat gtcgagaggg
     1261 ttcttgagag gggtgcccct cgctcggagc cacccgtcag tgttctgaca gacagaacag
     1321 tgacaccgac cgccgcggtc ccgcccccac tgtcagggtt ctgacagaca gaaccctgac
     1381 gggtccgctc gagcgaccac ccctcctctt gagacgaacc ctgacgggtc cgctcgagcg
     1441 accgggcacg ctccgttcgc cagcatcgca gccgcccgaa cacccgctgc taccctcccc
     1501 gcatgcccat cgtggtgcag aaatacggcg gaacctcggt cggcgacacc gaccgcatcc
     1561 ggggcgtcgc ggaccggatc gtccgcgcgc gcgagcaggg ccaggacgtc gtcgtcgtcg
     1621 tctcggccat gggcgagacc acggacgacc tcctcggcaa ggcctcggac atcacggccg
     1681 accccgagcc gcgcgagctc gacatgctct tgacctcggg cgagcgaatc gcgatgtccc
     1741 tcctcgccat cgccgtcaac gctcgaggct gccgggcggc ctcgtacacc ggctcgcagg
     1801 cgggcatcat caccgacacg cagcacggca aggcccgcat cgtggagatc cgcccgcaac
     1861 ggatccgcca atcactcgac gccggca
//
LOCUS       DATBRK010000176        25016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1472834, whole genome shotgun sequence.
ACCESSION   DATBRK010000176 DATBRK010000000
VERSION     DATBRK010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 25016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25016
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472834"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2030
                     /gene="cphA"
                     /locus_tag="VIH70_05240"
     CDS             <1..2030
                     /gene="cphA"
                     /locus_tag="VIH70_05240"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY8115799.1"
                     /translation="AAATKIGFPVVTKPLDGNHGRGVGLELRTEEDVRTGFRRAMPEA
                     RRAQVVVESFVTGNDYRVLVVGGRMVAVAERVPAHVVGDGTHTVSDLVEIANSDPRRG
                     IGHEKVLTRIRVDDAAIELLRKQGYGLDDVPPAEAFVKLAATGNMSTGGNSIDRTWEA
                     HEENVEIAEEAARVVGLDVAGIDFLAPDITEPVRETGGAIVEVNAAPGFRMHTNPTEG
                     EAQFVAKDVVDLLFPGGTPSRIPIVAVTGSNGKTTTTRMISHIFKGMGRSVGMTTTDG
                     IYIDERLVKRADASGPKSAQMVLQNPRIDFAVFETARGGILREGLGYGKNDVGVVLNV
                     TGDHLGLKGIDSLEQLAAVKQVVVEAVPKTGWAVLNADDELVAGMRKACSGSVILFSM
                     LEGNELLDRWVRRGGKAVVLEKGPRGEMMVIREGRRTMPIAWVHTLPATFEGRARMMV
                     QNAMAAAAAAHAAGAHLHDIRQGLRSFTTSIYQAPGRLNVFDLHGVKVLIDYAHNAAG
                     LEMLGDFVERLTTDAPNGGRPGQTSWSANLRVAVVATAGDRRDEDMRHLGRVAARHFD
                     DVIIREDRNTRGRKRGETAALILEGVREAIASGARAGNAEIVVDEMEAARKALDRSRP
                     GDLVVLCVDHATEVFRELESRRSLAAPTIFASTEGNGHVEESGGDPDLLGV"
     gene            2060..2374
                     /locus_tag="VIH70_05245"
     CDS             2060..2374
                     /locus_tag="VIH70_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY8115800.1"
                     /translation="MLERTRDELRAELATLTAAPRDPMGAVSFGKRVGEGTSQAVERI
                     AQVDAAKRLDAKLRDVERALEKLGEETYGVCDVCGSTIGEERLEAIPWAVLCIDHAAG
                     PR"
     gene            complement(2386..3456)
                     /locus_tag="VIH70_05250"
     CDS             complement(2386..3456)
                     /locus_tag="VIH70_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8115801.1"
                     /translation="MTDRPRDPITVALIGYGLAGAVFHGPLVSSTPGMSLATIVTGDP
                     GRRARASSKHPDAVLLDDVEALWERAADHDLVVVATPNRSHVPLALAALEAGLPVVID
                     KPVAPTAAEGRRLVAEAAERDLLLTVFQNRRWDGDFLTVRRLLSEDALGPIVRFESRF
                     ERWRPRVSEGVWRERGDPQEAGGLLFDLGSHLIDQAVVAFGPPRTVYAEVDRRRPHAE
                     VDDDVFIALEHGSGVRSHLWMSVLAAIRGPRIRLLGLRAAFEKFGLDVQEQALSEGAL
                     PDDPAYGREPPERWGRLSNGEDERTIETEPGAYQEFYRGVAASLREGTPPPVDPHDSV
                     MGLEIIEAAQESARTERVIAVG"
     gene            complement(3494..4438)
                     /locus_tag="VIH70_05255"
     CDS             complement(3494..4438)
                     /locus_tag="VIH70_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8115802.1"
                     /translation="MRIEGPRQVATLDVREPEATAGEVSVHVRAAAICATDRRLAMHG
                     TPTPRIPGHEIVGVLQDGTEVGVQPNVGCGRCASCALGLENRCPDHVDIGIQRDGGLA
                     EWIVVPERHAVPLDGFPFDLAPSIEPLACCLHAVAMLDVTSDEVAVVVGAGTLGIIGM
                     WALQAMGARVAVVQRSEARRRLAAELGADAALGPGDDVAAALGSPPVAALVTAPGVEA
                     LSWAIGNVDVGGRVHAFAGSPGGAPIDANLVHYRHLRLVGSTGSTVDDYGRARDLARS
                     GAVPLERLPSRTVSLDEVPGILLDSRPDPRTLRIVVRP"
     gene            4591..5298
                     /locus_tag="VIH70_05260"
     CDS             4591..5298
                     /locus_tag="VIH70_05260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8115803.1"
                     /translation="MNGLSGRVAIVTGASGGIGAAVARSLAGQGVRLGLASRSGGNLG
                     IDGSLASSCDVRDAGAVAAFVERVVVAFGRLDLVIANAGVGAYGPFLELDPAHLEEMI
                     DTNVKGTIYTIRAALPHLLESDGADIVTIASEAGRRGLPFEAVYCASKFAQVGFTRAL
                     DHELRPRGIRCANVCPGGVATEFAMGRGRTREMPELAGMMSAEDVAEAVLFVLTRPRN
                     HRILEMAFRPVTEDSWG"
     gene            5295..6281
                     /locus_tag="VIH70_05265"
     CDS             5295..6281
                     /locus_tag="VIH70_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8115804.1"
                     /translation="MSGDVVRWGVLSTAKIGIEKVIPATAAAARCEVVAIASRDLGRA
                     QTAASGLGIARAFGSYEDLLADPDIDAVYIPLPNHLHADWTIAAAVAGKHVLCEKPLA
                     TSAAEAELMIQACEAEGVLLMEAFMYRLHPTWEAVRELLANDRIGELRAVQSWFSYFN
                     DDPGDIRNIVDAGGGALYDIGCYCVNLSRMLFGAEPIRIQGSVTRDAVMGIDTLTSGI
                     LGFDDGMATFTCGIRAEPDQRVHIYGTGGRISVEIPFNIPPDRPTRVFVTAGGDPPVR
                     PDTQVLTFEPANEYTIQAERFASAVLDGTPAPIPPSDAVGNLRVIEELFRVG"
     gene            complement(6301..7800)
                     /gene="xylB"
                     /locus_tag="VIH70_05270"
     CDS             complement(6301..7800)
                     /gene="xylB"
                     /locus_tag="VIH70_05270"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEY8115805.1"
                     /translation="MSLAVGVDVSTTATKAVVQDTEGTVRAVGTAEYPYDTPHPLWSE
                     QDPALWWNATGVAVRAALDAAEADAAAVEAVGLTGQMHGLVALDARDEVLRPAILWND
                     QRTAAECDAIREIVGRRRLIATTGNDALPGFTAPKLLWLQRHEPEVWASISHVLLPKD
                     LVRLRLTGEHAVDRADGSGTILFDLAARDWSPEIVDALGIERRWLPPTYEGPMVTGTV
                     TEAAATSTGLLPGTPVIAGGGDQAAGAVGVGSVLPGVSSLSLGTSGVMFTTTEGPTIE
                     PEGRLHAFCHALPDRWHLMGVMLSAGGSLRWYRDALAPGVAFEELVEEAAQAPPGSDG
                     LLFLPYLTGERTPYPDPLARGAFVGLTVHHGRAHLTRAVLEGVAFGMRDSLELMLASG
                     IAPPTEIRATGGGIRSALWRQILADVLGAAIVTTSGAEGAAQGAATLAAVGAGWFDTV
                     QDACRALVRIGGATTPSEAAGAYTDAYARYRDLYPALAATFHSLGRAMG"
     gene            complement(7838..9001)
                     /gene="xylA"
                     /locus_tag="VIH70_05275"
     CDS             complement(7838..9001)
                     /gene="xylA"
                     /locus_tag="VIH70_05275"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY8115806.1"
                     /translation="MTYEPEPEHRFSFGLWTVGNVGIDPFGGPVREALSPVQLVELLA
                     EVGAWGVNLHDNDLVPIDASPHERDQIISDFRKALDATGLVVPMATTNLFKEPIFRDG
                     AFTANDARVRAYALQKTMHAIDLGVELGATTYVFWGGREGVETDSSKDPLVALRRYRE
                     SMDFLCAYVKDQGYDLRFALESKPNEPRGDIYLSTTGAMLAFIETLEHSEMVGVNPEV
                     AHEHMAGLNFTHHVAQAWSMGKLFHIDLNDQIPGRYDQDLRFGSANPKAAFFLVKLLE
                     DVGYAGSRHFDAHAYRTSDYEDVKAFARGCMRTYLILKEKAERWNTDPEIQELVAQVN
                     ADDGTITPDLGPYTSERAQGLRDLEIDRRTVAARGQPYERLDQLTVDLLLGVR"
     gene            complement(9038..10459)
                     /locus_tag="VIH70_05280"
     CDS             complement(9038..10459)
                     /locus_tag="VIH70_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115807.1"
                     /translation="MDARRADPMTGARLIEVDLPDFGMPQTVPEIPASIYAGRLERLR
                     ERAAEDGYDRIVVYADREHSANLAYLTGFDPRFEEAMLVVGPSGEPAVLVGNECFGIA
                     GAAPLSMRRHLFQDFSLPSPPRDRSRTLSEILADEGIVDGTRIGVIGWKTFAHRGTLE
                     VPAYIADELRRVTPSGWVENAGHLLIDAADGQRVLNEVEQLAAFEYAACQTSSGVRRL
                     LHELQPGMRENDAVALLGWNGSPLSCHLMLSSGDRATLGLLSPTDREIERGDRFTVAF
                     GIWGALNCRAGFVVEDAAELPAAIRDYVDKLVGPYFDAIAEWYGALRIGQTGGVLYEI
                     VQRRLGDPFFGIFLNPGHQIHLDEWVNSPISAGSTIQLRSGMAFQVDVIPATGTEYFT
                     TNIEDGLALADESLRSEFATRYPDAWSRIRARRAFMADLLGIELHPDVLPFSNIPAWL
                     PPFLLRPERVMALPGWERVRAAE"
     gene            complement(10432..12168)
                     /locus_tag="VIH70_05285"
     CDS             complement(10432..12168)
                     /locus_tag="VIH70_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEY8115808.1"
                     /translation="MQRVRSATDEGASGPPVARPNAGRPERSRSNALWLVLGLIGGAL
                     LALAVVLAIGSALKRGGVAAAAAPRFVEESTAAGIDHVYGGEFPYFVGGGVATFDCDG
                     DRKPDLYFAGGANPAALYRNESPIRGALRFAKVSDRTTDLTRVLGAYPIDIDGDGLTD
                     LVVLRNGENVLLRGRGGCRFERANERWGYQGGDALTAAFSATWEGSAALPTLAFGNYL
                     DLAKADRSECADSELVRPGPGQRAYAEPIPLAPGYCTLSVLFSDWDRSGRADLRMAND
                     RHYYLDGEEQLWRITPGETPRLYTRAEGWQRLVIWGMGIANYDVTGDGLPEIYLTSQA
                     DNKLQTLANATGRPTYRDIAIDRGVTATRPYTGGDPLPSTAWHPEFQDVNDDGFVDLF
                     VSKGNVEAQQDFASRDPSDLLIGQADGTFVEEGLAAGIASFVRGRGAAVVDLNLDGML
                     DIVQVNRRVNVSLFRNVGSGTAAEPQPMGNWVAVRPEQPGPNRDAIGSWIQVRFGDRT
                     AERELTIGGGHASGELGWVHVGLGDADAAEVRIRWPDGEVGPWQEVAANGFVTIPRAG
                     EPTAWTPAAPTP"
     gene            12609..13568
                     /locus_tag="VIH70_05290"
     CDS             12609..13568
                     /locus_tag="VIH70_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8115809.1"
                     /translation="MGVSWNNYNEERWAKWDEPAIQQALQACGAEYISSDAGSSAEQQ
                     LADVENLISQGANSLIILAQDGEAILPAVQSALDQGIPVVAYDRLIENPGALYVTFDN
                     VEIGRLEAEAVYDVVPKGNYVFIKGSSTDANADFLRQGQQEVLQDALDSGDIKNVGES
                     YTDNWDPAVAQTNMEQFLTDNNNNVDAVLAENDGMAGGVVAALEAQGLAGEVAVSGQD
                     GDAAGLNRVALGTQTVDVWKDARELGKAAGESACAMAQGTALQDVANTEAFDSPGGNS
                     MTSIFLTPQAITQDNLDVVLDAGWISEGDLCQGVDPGTVEVCG"
     gene            13753..14979
                     /locus_tag="VIH70_05295"
     CDS             13753..14979
                     /locus_tag="VIH70_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018446785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8115810.1"
                     /translation="MFRAAEVDSRLVGLLGGLAIIWIIFAVITPNHTFLTPRNLWNLS
                     VQTAVVAIMATGMVLIIVTRNIDLSVGAVMAAVGMAMAVVQHDWLPEWIGFDHPLTWV
                     IAVAVGLALGAAIGAFQGTIIAYVGVPSFIVTLGGFLVWRGVAWWMASGQTIAPMDTL
                     FRKLGGGSTGTVGGTWSWILGIAACVGVVLLQFTKRRQRDRYGFRLRPMWAEVGIVVI
                     VCAAILVSVGFLNRYYLPPALAAQAGKAGQNISLGLAYPVLIAIGVGIVMTFIATRRR
                     FGRYVYGIGGNPEAAELAGIKTRGTIVKTFTLMGLLVGLAAAVQIARLNAAVSSLGTG
                     MELNVIAAAVIGGTSFSGGIGTIVGALLGALVMQSLVSGMVLMGIEAPLQDIVVGIVL
                     VLAVTLDTIVRRRAAR"
     gene            14976..15761
                     /locus_tag="VIH70_05300"
     CDS             14976..15761
                     /locus_tag="VIH70_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8115811.1"
                     /translation="MTDRTGTPLVELRDIRVAFGGVHAVDGVTVDLYPGEVVGLVGGN
                     GAGKTTLIRTLSGAHPADSGQILIEGEPVTISHPRDAKALGVETIYQALALADNIDAP
                     GNVFLGRELTTRFGNLDDSAMEDATRKLMGRLNPKFQNFVVPVASLSGGQRQSVAIAR
                     AVHFNARILIMDEPTAALGPAETRQVGDLIKQLREEGLGIFLISHDIHDVYDLSDRIS
                     VMLQGKLVGTVNKDDVTTDEVLAMIILGKLPGEVTQKDLAELH"
     gene            complement(15768..17489)
                     /locus_tag="VIH70_05305"
     CDS             complement(15768..17489)
                     /locus_tag="VIH70_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115812.1"
                     /translation="MLGDALNEPHPPIAQTSARSVTDAPATPEVAPGRAAPEPPAEPA
                     GRTVRMLGRTYPFVAPNIRDPRLHLAAVIISIHVLGQIGLGFRVSVPQILVAILVCAV
                     IEVGWTLSKIGTLVWPASAMLTGSGVALILRLSDMQSNDHWSWRGWYVFALVAGLSLL
                     TKYLIRYRGSHIFNPSNVGLVAAFLLLGSARIEPLDFWWAPFDGWMIAAYLIILVGGL
                     LITARLHLLGMSAAFWLTLAVGMGVLAASGHCMTARWSFEPVCGAHFWWVIVLSPEIL
                     IFLFFMITDPKTTPDGRVARVVFGICVAMVCTLLIAPQTTEFGAKVALLSGLVVLCVA
                     RLFFEKLLPAPGSQRDRPAAYLVGLGRRGEGYLAPGRALARGAVAGAAIVLVGTSVVA
                     AGAPARESYVSASASVPSSASEVPELSVIVDPSTLPRVSVDPEVVDRGDLTRSDAQAI
                     ALTLAENLEVEAQALRRGEKSLLSMVDDGQRQTTLERRVDEVIETGRAVVPHYAFDEL
                     HVVLVQPEGQAGLSLGFQARGTVEEVTYRVDGSERSRSSSPFSKTFVLSRPTGDRWLL
                     VEVGPNP"
     gene            17557..18618
                     /locus_tag="VIH70_05310"
     CDS             17557..18618
                     /locus_tag="VIH70_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY8115813.1"
                     /translation="MIPTEPIGSIPRPKNLIDGQAAFAEGTISPEELRALQDEALAET
                     IERFEETGSPVISDGEQTKSSFVTYPLDGVTNLAADGVVIPFADGHTRQLPTLASGPF
                     RFGTYAASYLLEAKRRAHVPVKQAVIAASALSLLYPQDGIEGYSRDAFLDDLVGESEK
                     DIRASLDAGAHKVQIDFTEGRLACKLDPSGGLLRDFVALNNRVLERFSDDERSRIGVH
                     TCPGGDMDSTHSADVDYAQLLPDLFQLNVGNVYVEMAGEKDRARVLDIIAEHLGPEQR
                     IFVGVIDPIDPSVESREEVRDRVLEAAERLPADRLGTTDDCGFAPFADDTSTSRDVAF
                     EKIRARIEGTRLAGERLGI"
     gene            complement(18615..20312)
                     /locus_tag="VIH70_05315"
     CDS             complement(18615..20312)
                     /locus_tag="VIH70_05315"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY8115814.1"
                     /translation="MAPPRADLVELRVLDGPNLYFPRPAVKLTIAVGGWLDVPEERLE
                     TALERVGATGRPGRPGSDQRRRTISRLAARVTWRLATASRVRLAVRSRPGPERDQVVV
                     AFPWRRRGAAEALGREVAPLLHEVGGRRSVDRLLAEAAERVAAAEPGNEPDVPEPEIP
                     VVSVTGTNGKTTTVRLLAHLVRSAGRTVAYSSTDGVYRGDGELIEEGDYSGFGGAARA
                     LAEAPDVAVLETARGGILLRGIGVMHNDVAVVTNVSEDHLGLHGIQTIDQLSEVKATI
                     TRITRADGWDVLNADDPRVLSMRRAATGRPWLCSLDPWHPAIRDTLADGGRATVPLDG
                     WMTVLDGSSSRPLVELIDVPVTIAGVSRHNVMNAMQAASAALAIGLPERAVVKGLKTF
                     VTDPERNPGRANLFELDGRVIVVDYAHNEAGMMGFAEICDGLRRAGAEIWLVICAAGD
                     RTDQILHDFGYRAARGSDHLAIAELLRYLRGRERQSTIDRLIAGAKDGGAVEVDVYAH
                     ELAGLQGVLARSGRGDVIGVTALGMRTEVFGWLEGAGARRLTPARVKQLVRRAERRP"
     gene            complement(20327..21592)
                     /locus_tag="VIH70_05320"
     CDS             complement(20327..21592)
                     /locus_tag="VIH70_05320"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8115815.1"
                     /translation="MPNDESKRSLVRRIAVDVTPLRASRDFRRLWLGLLASTFGSQFT
                     IVATFLQVYGLTRSTAAVGLIGLVGFLALVAGTLAGGTFLDAFDRRKILISAQIGYLA
                     SSGLLFAGAVHGDPPLALIYVAVAGIAATSAIDGPTRSAMTPRLLDRSQLPAAAALNQ
                     IIWNAAGLVGPALAGIVVAGFGFSWAYAIDLISIGLLLLASISIRPMPPAEGERTAVG
                     WRAVREGFAFLRGRQVLQSTFVIDIIAMVFGMPRALFPVLALTQFHRGATVVGFLFAA
                     PAVGAFVGAATSGWVGRIRHQGLAVIWAVVVWGAGIAAFGLVGSHLWLGLGFLAIAGA
                     ADVISAVFRNTILQVTVPENLRGRLFGIHILVVTGGPRLGDLEAGLVAAAFSPLVSVV
                     SGGLLCIAGAGLVAWRVPAFRRYHAGDPG"
     gene            complement(21647..22336)
                     /locus_tag="VIH70_05325"
     CDS             complement(21647..22336)
                     /locus_tag="VIH70_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8115816.1"
                     /translation="MTRTRAHPPFGVAVDLVVLTVIDGAFQALLIERGEEPFAGELAL
                     PGGFVHVDEGLDEAAARELAEETGIRVAHLEQLATYGDPDRDPRMRVVSVAYLAMVPE
                     PPSPTAGTDAATAGWKRVRDLAVNQLAFDHDRILEDGIERARGKLEYTTLATSFCPPE
                     FTIAELREVYEAIWDESLDPRNFSRKVLSSAGFVVPTQATTTRSGGRPARLYRAGRAT
                     ELAPPVRRPGD"
     gene            22406..22984
                     /gene="pnuC"
                     /locus_tag="VIH70_05330"
     CDS             22406..22984
                     /gene="pnuC"
                     /locus_tag="VIH70_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEY8115817.1"
                     /translation="MTASDLLEWIGFLSGVLCVWWVVKERIANWPMGIVNCLAWLILL
                     WINAIYLNALLQLVYIVISVYGWWHWLWGGEARDSLPVRYATSAERLRAAAVIAVGGV
                     AGARLMVLAGDAAPIWDALTTMTSIVAIYWQARKIYESWILWLSVDIIYFALYGSQDL
                     WLTALTQVAFGAMCMVGLRDWRRSMQREAVAA"
     gene            22981..24024
                     /locus_tag="VIH70_05335"
     CDS             22981..24024
                     /locus_tag="VIH70_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8115818.1"
                     /translation="MTHGVVVGKFYPPHRGHRHLIDVAVSQSDRVTVLVVDGRDEDPP
                     AEARAGWLRELHPDATVKVTRDIERDEDSVAWARATRQAVGGQTIDSVFNSEPYGSAW
                     AAAISDQQGSDCENVFVGRGFVQISATQVRSDPAHYWDYIDAPVRAHYVKRVCIVGAE
                     STGSTTLARSLAAACETIWVPEYGRTYTVEKFRIGTGDRWLPSDFLRIALEQNALEDR
                     MAREAPVGIMFCDTDSLATALWEEVYLGSTSDAVWRAADAHGAPYVLYLLTDHAGIPW
                     EDDGLRLGDETRAAMTQRFEEVLSARGLPWMKVSGSQKERLHQAVEGIRSSVEHDFTF
                     GPVDLHDREAINL"
     gene            complement(24027..>25016)
                     /locus_tag="VIH70_05340"
     CDS             complement(24027..>25016)
                     /locus_tag="VIH70_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8115819.1"
                     /translation="GPKKTMFANSGAEAVENAVKIARKATGRQAIVTFDHAFHGRTLL
                     AMSLTAKVMPYKQGMGPFAPEIYRLPFAYPYRWPGGPERCAEEALDYALDEIHKHIGE
                     DNVAAVILEPIQGEGGFIVPAPGFVKGLAEFCSEHGILFIADEIQSGMGRAGRWFAIE
                     DEGVVPDLITSAKSLGGGLPISAITGRADVMDSVHVGGLGGTYGGNPVAAAAALAVLD
                     LIEKENLLERSRTLGERVLGRLREMQDRHQLIGDVRGRGMMTAIELVSDRATKEPLSG
                     PDGNAIVRRCLEEGVVILKAGTYDNVIRLLPPITIEEGLLDEGLETLDAAIGA"
BASE COUNT         3846 a         8687 c         8868 g         3615 t
ORIGIN      
        1 tcgccgcggc caccaagatc gggttccccg tggtcaccaa gccgctcgac ggcaaccacg
       61 ggcgcggcgt cgggctcgag ctgcgcaccg aggaggacgt ccggaccggg ttccggcggg
      121 cgatgcccga ggcccgtcgc gcgcaggtcg tcgtcgagag cttcgtgacc ggcaacgact
      181 accgggtcct cgtcgtggga ggtcggatgg tcgcggtcgc cgaacgcgtt cccgcgcacg
      241 tcgtgggcga cggcacccac acggtctccg acctggtcga gatcgcgaac tcggatcccc
      301 gccgcgggat cggacacgag aaggtgctca cgcggatcag ggtcgacgac gcggcgatcg
      361 agctgctccg caagcagggg tacggcctgg acgacgtgcc gcccgccgag gcgttcgtga
      421 agctcgcggc cacgggcaac atgtccacgg gcgggaactc gatcgaccgg acgtgggagg
      481 cgcacgagga gaacgtcgag atcgccgagg aggccgcccg cgtcgtcggc ctcgacgtgg
      541 ccgggatcga cttcctcgcg ccggacatca ccgagcccgt gcgcgagacg ggcggcgcga
      601 tcgtcgaggt gaacgccgca ccgggattcc ggatgcacac gaacccgacc gagggcgagg
      661 cccagttcgt cgcgaaggac gtcgtcgacc tgctgttccc cggcggcacg ccctctcgga
      721 tcccgatcgt cgccgtcacc ggttcgaacg gcaagaccac gaccacgcgg atgatctcgc
      781 acatcttcaa ggggatgggc cgctcggtgg gcatgaccac gaccgacggc atctacatcg
      841 acgagcgtct ggtcaaacgc gccgacgcga gcggaccgaa gtcggcgcag atggtcctgc
      901 agaacccgcg gatcgacttc gccgtgttcg agacggcgcg aggagggatc ctgcgcgagg
      961 ggctcggcta cggcaagaac gacgtcggcg tggtcctaaa cgtgaccggc gaccacctcg
     1021 ggctgaaggg catcgactcg ctcgagcagc tcgcggccgt caagcaggtg gtggtcgagg
     1081 ccgtcccgaa gaccggctgg gccgtcctga acgcggacga cgagctcgtc gccgggatgc
     1141 gcaaggcctg cagcggttcg gtgatcctgt tctcgatgct ggaaggcaac gaactcctcg
     1201 atcgctgggt gcggcgcggg ggcaaggcgg tcgtgctgga gaagggtccg cgcggggaga
     1261 tgatggtgat cagggagggg cgccggacga tgccgatcgc ctgggtgcac acgctccccg
     1321 ccacgttcga gggacgcgcc cgcatgatgg tgcagaacgc gatggcggcc gcggccgcgg
     1381 cgcatgccgc gggcgcgcac ctccacgaca tccgtcaggg gctgcgctcg ttcacgacct
     1441 cgatctacca ggcgccgggg cggctgaacg tgttcgatct gcacggcgtc aaggtgttga
     1501 tcgactacgc gcacaacgcg gccggtctcg agatgctcgg cgacttcgtg gagcggctca
     1561 cgaccgacgc gccgaacggc ggccggccgg ggcagacgtc gtggagcgcg aacctgcgcg
     1621 tcgcggtggt cgcgacggcg ggagaccggc gcgacgagga catgcgtcac ctcgggcgcg
     1681 tcgccgcccg gcacttcgac gacgtgatca tccgggagga tcgcaacacg cgcggacgca
     1741 agcgcggcga gacggcggcg ctcatcctcg agggggtccg cgaggcgatc gcttcgggcg
     1801 cgcgcgcggg gaacgccgag atcgtggttg acgagatgga agcggcccgc aaggccctcg
     1861 accggtcgcg cccgggcgac ctcgtcgtgc tgtgcgtcga ccacgccaca gaggtcttca
     1921 gggagctcga atcccggcgc tcgctcgccg cccccacgat cttcgcctcg accgagggca
     1981 acggccacgt ggaggagtcc ggcggcgatc ccgacctgct cggcgtctga ccgcgatgga
     2041 tccctcacgg gttcggaagg tgctcgaacg cacccgcgac gagcttcggg ctgagctcgc
     2101 gacgctcacc gccgcgcccc gcgatccgat gggcgccgtg tcgttcggca agcgcgtggg
     2161 cgagggcacg tcgcaggcgg tggagcgcat cgcgcaggtc gacgcggcga agcggctcga
     2221 cgcgaagctc cgcgacgtcg agcgcgcgct cgagaagctg ggtgaggaga cctacggggt
     2281 ctgcgatgtg tgcggatcga ccatcggaga ggagcgcctc gaggcgatcc cgtgggcggt
     2341 cctctgcatc gaccacgccg ccgggccccg ctgagtggac gaacctcagc cgaccgcgat
     2401 cacgcgttcg gtgcgcgcgc tctcctgcgc ggcctcgatg atctcgagtc ccatgaccga
     2461 gtcgtgggga tccaccgggg gcggcgttcc ttcccgcagc gacgcagcga cgccccgata
     2521 gaactcctgg taggcgccgg gctcggtctc gatggtgcgt tcgtcctcgc cgtttgagag
     2581 gcggccccac cgctccggcg gttcgcgtcc gtacgcggga tcgtcgggca gcgccccttc
     2641 gctcagcgct tgctcctgca cgtccagccc gaacttctcg aacgccgcgc gcaacccgag
     2701 cagccgaatc cgcggcccgc ggatcgcggc gaggacgctc atccacaggt ggcttcgcac
     2761 acccgacccg tgctcgagcg cgatgaagac gtcgtcgtcg acctcggcat ggggtcggcg
     2821 gcggtcgacc tccgcgtaga cggtccgtgg cgggccgaac gccacgaccg cctgatcgat
     2881 caggtggctc cccaggtcga acaggaggcc gccggcctcc tgcgggtccc ctcgttcacg
     2941 ccagaccccc tcgctgaccc gcggacgcca tcgttcgaac cgtgactcga agcggacgat
     3001 cggcccgagc gcgtcctccg agagcagccg gcggaccgtg aggaagtccc cgtcccatcg
     3061 acggttctgg aagaccgtga ggagcagatc gcgctcggcc gcctcggcca ccaaccgacg
     3121 gccctccgcg gccgtcggcg ccacgggctt gtcgatcacg accgggagtc cggcctcgag
     3181 cgcggccagc gcgaggggca cgtgcgagcg gttcggcgtc gccacgacga cgaggtcgtg
     3241 atcggccgct cgctcccaca acgcctcgac gtcgtcgagc agcacggcgt ccggatgttt
     3301 cgagctcgcg cgggcccgcc gcccgggatc gccggtcacg atcgtcgcga gcgacatgcc
     3361 gggggtggac gagacgagag gcccgtggaa gaccgcgccc gcgaggccgt acccgatcag
     3421 cgcgaccgtg atcggatccc gcggacgatc agtcactcgg caaccgcgtc cgacgagcca
     3481 tggtcggcgt agcctacggt ctcaccacga tcctcagcgt ccgggggtcg gggcgggagt
     3541 ccagcaggat cccagggacc tcgtcgagcg agaccgttcg cgacggcagt cgttcgagcg
     3601 ggaccgctcc cgacctggcc aagtcgcggg cgcgtccgta gtcgtcgacg gtcgagcccg
     3661 tgctccccac cagccggagg tggcggtagt gcacgaggtt ggcgtcgatg ggggcgccgc
     3721 cgggggaacc ggcgaacgcg tggacccgac ccccgacgtc gacgttcccg atcgcccacg
     3781 aaagggcctc gacgcccggg gcggtcacga gcgccgccac cggaggggat ccgagcgccg
     3841 cggcgacgtc gtccccgggc ccgagcgccg cgtccgcgcc cagctcggcc gcgagtcggc
     3901 gccgagcctc ggatcgttgc acgacggcca cccgcgcgcc catcgcctga agcgcccaca
     3961 tcccgatgat cccgagcgtg ccggcgccca cgacgacggc gacctcgtcg ctcgtcacgt
     4021 cgagcatcgc caccgcgtgc aggcagcagg cgagcggttc gatcgagggc gcgaggtcga
     4081 acgggaagcc gtcgagcggc accgcgtggc gttcgggcac gacgatccac tcggccagac
     4141 cgccgtcgcg ctggatcccg atgtccacat gatccgggca ccggttctcc agtccgagcg
     4201 cgcacgacgc gcatcgaccg cagcccacgt tcggctgcac gcccacctcc gtcccgtcct
     4261 ggaggacgcc gacgatctcg tggccaggga tcctcggcgt gggcgttccg tgcatcgcca
     4321 gtcgtcggtc ggtggcgcag atcgcggccg cacggacgtg gacggacacc tcgcctgccg
     4381 tcgcctccgg ttcccgcacg tccagcgtgg cgacctgccg cggaccttcg atccgcacgg
     4441 ccctcatccc ccgagcttgc catgaagctc gccacggcgc ggcgtcaccc gacgctcggg
     4501 tacgttgtcg aacgacgccg ggtgaaccgg gtcacccgcg atcctcgagg ggtgaggtgc
     4561 gcgaccgcgc gagcaggaaa ggatgacggc atgaacggac tgagcggacg ggtcgcgatc
     4621 gtgacgggtg caagcggcgg gatcggggcc gccgtggcga gatcgctcgc ggggcagggt
     4681 gttcggctcg ggctggcatc gcggagcggc gggaacctcg ggatcgacgg gtcgctcgcc
     4741 tcttcctgcg acgtccgcga cgccggcgcg gtcgcggcgt tcgtcgagcg ggtggtcgtc
     4801 gccttcgggc ggctcgacct ggtgatcgcg aacgcgggcg tcggggcgta cggcccgttc
     4861 ctcgagctcg atccggcaca tctcgaggag atgatcgata ccaacgtcaa gggcacgatc
     4921 tacacgatcc gcgcggcgct gccgcacctg ctggagagcg acggggccga catcgtcacg
     4981 atcgcgtcgg aggcggggcg tcgcggcctg ccgttcgagg ccgtctactg cgcgtcgaag
     5041 ttcgcgcagg tcggattcac gcgcgccctg gaccacgagc ttcggccgcg gggcatccgg
     5101 tgcgcgaacg tgtgccccgg aggcgtggcg accgagttcg cgatgggccg gggtcgaacg
     5161 cgcgagatgc cggaactcgc ggggatgatg tccgcggagg acgtggccga ggccgtgctg
     5221 ttcgtgctga cgcgtccgag gaaccaccgg atcttggaga tggcgttccg gccggtgacg
     5281 gaggactcgt gggggtgagc ggcgacgtgg tgcgatgggg tgtcctctcg accgccaaga
     5341 tcgggatcga gaaggtcatc ccggccacgg ccgcggccgc acggtgtgag gtcgtcgcga
     5401 tcgcctctcg tgatctgggc cgtgcccaga ccgccgcgtc cgggctcggc atcgcccgcg
     5461 cgttcggctc gtacgaggac ctgctggccg atccggatat cgatgccgtg tacatccctc
     5521 tgcccaacca cctgcacgcc gattggacca tcgcggcggc cgtggccggc aagcacgtct
     5581 tatgcgagaa gccgctcgcg acgagcgcgg ccgaggccga gctgatgatc caggcctgcg
     5641 aggcggaggg cgtgctgctg atggaggcgt tcatgtaccg gctccatccg acctgggagg
     5701 cggtccgcga gctgctggca aacgaccgga tcggggagct gagggccgtc cagagctggt
     5761 tctcgtactt caacgacgac ccgggcgaca tccgcaacat cgtcgacgcg gggggaggcg
     5821 ccctgtacga catcggttgc tactgcgtga acctgtccag gatgctgttc ggcgccgagc
     5881 cgatccggat ccaaggatcg gtgacgcgcg acgccgtcat ggggatcgac acgctgacgt
     5941 cggggatctt gggcttcgac gacggcatgg caacgttcac gtgcgggatc cgtgccgaac
     6001 ccgatcagcg cgtccacatc tacggcaccg ggggtcggat ctccgtggag atcccgttca
     6061 acatcccgcc ggaccggccg acgcgggtgt tcgtcaccgc cggcggcgat ccgccggtcc
     6121 ggcccgacac gcaggtgctg acgttcgaac cggccaacga gtacacgatc caggcggagc
     6181 ggttcgcctc ggcggtgctc gacggcacgc cggcgccgat cccgccgtcg gacgccgtcg
     6241 gcaacctccg ggtgatcgag gagctcttcc gcgtcggttg agccccgccg cgctcgcgga
     6301 tcagcccatc gcgcggccca gggagtggaa ggtcgcggcg agcgccggat agaggtcgcg
     6361 gtacctggcg tacgcgtccg tgtaggcgcc cgcggcctcc gagggcgtgg tggccccgcc
     6421 gatccgcacg agcgcccggc acgcgtcctg gaccgtgtcg aaccacccgg ccccgacggc
     6481 ggcgagcgtg gcggcgccct gcgcggcgcc ctcggcgccg gaggtcgtca cgatcgccgc
     6541 tccgagcacg tcggcgagga tctgccgcca cagggcgctc cggatgcccc cgccggtcgc
     6601 ccggatctcg gtcggggggg caatgcccga cgcgagcatc agctcgagcg agtcgcgcat
     6661 gccgaacgcc acgccctcga gcaccgcccg cgtgaggtgg gcgcgaccgt ggtggacggt
     6721 caggccgacg aacgcgccgc gggcgagggg atcggggtac ggcgtgcgct cacccgtgag
     6781 atacggaagg aacaggaggc cgtcgctccc gggcggcgcc tgcgcggcct cctcgacgag
     6841 ctcctcgaac gcgacgccgg gggcgagcgc atcccggtac cagcgcaggc tccctcccgc
     6901 cgagagcatc actcccatca ggtgccatcg gtccgggagg gcgtggcaga acgcgtggag
     6961 tcggccctcc ggctcgatcg tgggcccctc ggtcgtggtg aacatcacgc ctgaggtccc
     7021 gagcgacagc gacgagaccc cgggcaggac cgaaccgacc ccgaccgcgc ccgccgcctg
     7081 gtcgcccccg ccggcgatca cgggcgtccc agggagcagc ccggtcgagg tcgcggcggc
     7141 ctccgtgacc gtgcccgtga ccatcggtcc ctcgtaggtc gggggcaacc acctgcgctc
     7201 gatcccgagc gcgtcgacga tctcggggga ccagtcccgg gccgcgaggt cgaacaggat
     7261 cgtccccgaa ccgtcggccc ggtcgacggc gtgctctccc gtgagtcgga ggcggacgag
     7321 gtccttggga agcagcacgt gtgagatcga tgcccacacc tcgggctcgt ggcgttgcag
     7381 ccacaacagc tttggtgccg tgaacccggg cagtgcgtcg ttgcccgtgg tcgcgatcag
     7441 gcgccgacga ccgacgatct cgcggatcgc gtcgcattcg gcggcggtgc gctggtcgtt
     7501 ccacaggatg gccgggcgaa ggacctcgtc acgggcatcc agcgcgacga ggccgtgcat
     7561 ctgtcccgtg agccccaccg cctccaccgc cgcggcgtcc gcctccgccg cgtcgagcgc
     7621 cgcccgcacc gcgacccccg tggcgttcca ccagagggcg gggtcctgct cgctccagag
     7681 cggatggggc gtgtcgtacg ggtactcggc cgtcccgacc gccctcacgg tcccctcggt
     7741 gtcctgcacg accgccttgg tcgccgtggt ggagacgtcc acccccaccg cgaggctcac
     7801 gggagcaact cacctggggc ccgcgtcccc ccgggcgtca ccgaaccccg agcaggaggt
     7861 cgaccgtgag ctggtcgagc cgctcgtacg gctggccgcg tgcggcgacc gtccgccgat
     7921 cgatctcgag atcccgcagg ccctgggcgc gttccgacgt gtagggaccc aggtcgggcg
     7981 tgatcgtgcc gtcgtcggcg ttgacctgcg cgacgagctc ctggatctcg gggtcggtgt
     8041 tccagcgctc cgccttctcc ttcaggatca ggtacgtgcg catgcacccg cgcgcgaagg
     8101 ccttcacgtc ctcgtagtcc gaggttcggt aggcgtgggc gtcgaaatga cggctgcccg
     8161 cgtagcccac gtcctcgagg agcttcacca ggaagaacgc cgccttcggg ttggccgagc
     8221 cgaaccgcag gtcctggtcg tagcgccccg ggatctggtc gttcaggtcg atgtggaaca
     8281 gcttgcccat cgaccaggcc tgcgcgacgt ggtgggtgaa gttcaggccc gccatgtgct
     8341 cgtgcgcgac ctccgggttc acgccgacca tctccgagtg ctcgagcgtc tcgatgaagg
     8401 cgagcatggc acctgtcgtg gagaggtaga tgtcgccgcg cggctcgttg ggcttggact
     8461 ccagcgcgaa gcggaggtcg tagccctggt ccttcacgta ggcgcacagg aagtccatcg
     8521 actcccggta gcggcgcagg gcgaccaacg ggtccttgga ggagtcggtc tcgacgccct
     8581 cgcgcccgcc ccagaagacg taggtggtcg cgccgagctc gacgccgagg tcgatcgcgt
     8641 gcatcgtctt ctgcagcgcg taggcgcgga ccctcgcgtc gttggccgtg aacgcgccgt
     8701 cgcggaagat cggttccttg aacaggttcg tcgtcgccat cggcacgacc aggccggtgg
     8761 cgtcgagcgc cttgcggaaa tcggagatga tctggtcccg ctcgtggggg ctggcatcga
     8821 tcgggaccag gtcgttgtcg tgcaggttga cgccccacgc cccgacctcc gcgagcagct
     8881 cgacgagctg gacgggggac agggcctccc ggacgggtcc gccgaacggg tcgatgccca
     8941 cgttgccgac cgtccacagt ccgaacgaga agcggtgctc gggttcgggt tcgtacgtca
     9001 tctcgccatc acctccctcg aggttctgcg attcaggcta ctccgccgcg cgcacgcgtt
     9061 cccagccggg cagggccatg acccgctccg gtcgcaggag gaagggcgga agccacgccg
     9121 ggatgttcga gaacggcagc acgtcgggat gcagctcgat cccgaggagg tcggccatga
     9181 aggcccgccg ggctcggatc cgcgaccacg cgtcggggta ccgcgtcgcg aactcggacc
     9241 gcagcgactc gtcggcgagc gcgaggccgt cctcgatgtt cgtcgtgaag tactcggtcc
     9301 ccgtcgccgg gatgacgtcg acctggaacg ccatgcccga acgcagctgg atcgtggatc
     9361 cagccgagat cggcgagttc acccactcgt cgaggtggat ctggtgtccc gggttcagga
     9421 agatgccgaa gaacgggtcg ccgagtcgcc gctgcacgat ctcgtagagt acgccgcccg
     9481 tctggccgat ccgaagggcc ccgtaccact ccgcgatcgc gtcgaagtag ggccccacca
     9541 gcttgtcgac gtagtcgcgg atcgccgccg ggagctccgc cgcatcctcc acgacgaacc
     9601 ccgcccggca gttcagggcg ccccagatcc cgaacgccac ggtgaaccgg tcgccgcgct
     9661 cgatctcccg gtcggtgggg ctgagcaggc cgagcgtcgc ccggtcgccg ctcgagagca
     9721 tcaggtggca ggagagcggg ctgccgttcc acccgaggag cgcgaccgcg tcgttttcgc
     9781 gcatccccgg ctgcagctcg tggaggagcc gtcggacgcc gctcgacgtc tggcacgccg
     9841 cgtactcgaa cgccgccagc tgctcgacct cgttcagcac gcgctggccg tcggcggcgt
     9901 cgatcagcag gtgtcccgcg ttctcgaccc accccgaagg cgtcacgcgg cgaagctcgt
     9961 ccgcgatgta ggcgggcacc tcgagcgtgc cgcggtgggc gaaggtcttc cagccgatca
    10021 cgccgatcct cgtgccgtcc acgatgccct cgtcggcgag gatctcggag agcgtgcgcg
    10081 agcggtcgcg cggcgggctc ggcaggctga agtcctggaa gaggtggcgt cgcatcgaga
    10141 gcggcgccgc cccggcgatc ccgaagcact cgttgccgac caggacggcc ggctcacccg
    10201 aggggccgac gacgagcatc gcctcctcga aacgaggatc gaatcccgtg aggtacgcga
    10261 ggttcgcgct gtgctcccga tccgcgtaga cgacgatgcg gtcgtagccg tcctcggcgg
    10321 cgcgctctcg gaggcgttcg agccggccgg cgtagatcga ggccgggatc tcgggcacgg
    10381 tctggggcat cccgaagtcg ggcaggtcga cctcgatgag gcgcgcgccg gtcatggggt
    10441 cggcgcggcg ggcgtccacg cggtcggctc ccctgcgcgc gggatcgtga cgaagccgtt
    10501 cgcggccacc tcctgccagg ggccgacctc gccgtcgggc caccggatcc ggacctcggc
    10561 cgcgtcggcg tcgccgagcc cgacgtggac ccacccgagc tcgccgctcg cgtggcctcc
    10621 gccgatcgtg agctcccgct cggcggtgcg gtcgccgaac cgcacctgga tccacgagcc
    10681 gatcgcgtcg cggttcgggc ccggctgctc ggggcgcacc gcgacccagt tgcccatcgg
    10741 ctggggttcg gcggcggtgc ccgagcccac gttgcggaac aggctcacgt tcacgcgacg
    10801 gttcacctgc acgatgtcga gcatgccgtc gaggttgagg tcgacgacgg ccgcgccgcg
    10861 gcccctgacg aagctcgcga tgcccgcggc gaggccctcc tcgacgaacg tcccgtccgc
    10921 ctgcccgatc agcaggtcgc tcgggtccct cgaggcgaag tcctgctgcg cctcgacgtt
    10981 gcccttggag acgaacaggt cgacgaaccc gtcgtcgttc acgtcctgga actcggggtg
    11041 ccacgccgtc gagggcagag gatcgccgcc cgtgtacgga cgcgtcgcgg tcacgcccct
    11101 gtcgatcgcg atgtcccggt aggtcggccg tccggtcgcg ttcgccagcg tctggagctt
    11161 gttgtctgcc tggctcgtca ggtagatctc gggcaggccg tcgccggtga cgtcgtagtt
    11221 ggcgatgccc atcccccaga tcaccagccg ctgccatccc tccgcgcggg tatacaggcg
    11281 cggggtttcg cccggggtga tgcgccacag ctgctcctcg ccgtcgaggt agtagtgccg
    11341 gtcgttcgcc atccgaaggt ccgcgcgacc ggagcggtcc cagtcgctga acagcacgga
    11401 cagcgtgcag tatccgggcg cgagcgggat cggttcggcg tacgcccgtt gacctggccc
    11461 cggccgcacg agctcgctgt cagcacattc ggagcgatcg gccttcgcca ggtcgaggta
    11521 gttcccgaac gcgagcgtcg gcagcgcggc cgaaccctcc cacgtcgcgc tgaacgcggc
    11581 ggtcaacgcg tcgccgccct ggtagcccca cctctcgttc gcgcgctcga accggcagcc
    11641 gccgcggccc cgcaggagga cgttctcccc gttccgaagc accacgagat cggtcagccc
    11701 gtcaccgtcg atgtcgatcg ggtaggcccc gaggacgcgg gtgagatcgg tcgtccgatc
    11761 ggagaccttc gcgaaccgca gggcaccgcg gatcgggctc tcgttccggt agagggcggc
    11821 cgggttggcg ccgcccgcga aatacaggtc gggcttgcgg tcgccgtcgc agtcgaaggt
    11881 cgccacgccg ccgcctacga agtaggggaa ctcgccgccg tacacgtggt cgatgcccgc
    11941 ggcggtggac tcctcgacga agcgtggcgc cgcggccgcc gccacaccac cacgcttcag
    12001 cgccgagccg atcgccagca cgacggcgag cgcaaggagc gcgccgccga tgagcccgag
    12061 gaccagccac agcgcgttgg atcgactccg ctccggacgt cccgcgttcg gcctggcgac
    12121 gggcggtccg ctggcgccct catcggttgc cgacctcaca cgttgcatcc gtcctgcctc
    12181 gcgcacgtgg tgggcgcgct cgcatcccgg atcgtgcgag gcgggatcgt cgcgcggggt
    12241 cgcagagtat ccggggctcc gaacgggcgc aacggctcgc gcgacctgcg gggacggatt
    12301 gcgcgctttc cccgaatatg ccatgttggc gcgcggccca ggcacagggg accgagcgcg
    12361 cccatccttc gacgggacgg tcgtcacgcg tacccgcgaa gccgcagggg ccgtccgttt
    12421 cgtacaacgg ggaaggtcgg cacgtcatcg ggacgtccgt ccagaagggg gggtgtgcgt
    12481 tgaagcgtcg attgctggcg ttgctcgccc tgtcggttct gctgctcgcc gcgtgttcga
    12541 acgatgacga gggcggcggt ggagcgacgg gagcgacagg aggggcctcc ggcggcggga
    12601 acgtcaccgt gggggtttcc tggaacaact acaacgagga acggtgggcc aagtgggacg
    12661 agcccgcgat ccagcaggcc ctgcaggcct gcggcgctga gtacatctcc tccgacgccg
    12721 ggtcgtccgc cgagcagcag ctggccgacg tcgagaacct gatctcgcag ggcgcgaact
    12781 cgctcatcat cctggcccag gacggtgagg cgatcctgcc cgcggtgcag agcgcactcg
    12841 accagggcat ccccgtcgtc gcgtacgacc ggctgatcga gaacccgggc gcgttgtacg
    12901 tcacgttcga caacgtcgag atcgggcggc tggaagcgga ggccgtctac gatgtggtcc
    12961 ccaagggcaa ctacgtgttc atcaagggga gttcgacgga cgcgaacgcc gacttcctcc
    13021 ggcaaggcca gcaggaggtc ctgcaggacg cgctcgactc gggtgacatc aagaacgtcg
    13081 gcgagtccta caccgacaac tgggacccgg cggtcgcgca gacgaacatg gaacagttcc
    13141 tgacggacaa caacaacaac gtcgacgccg tcctcgcgga gaacgacggc atggccggcg
    13201 gcgtcgtggc cgcgctcgag gcccagggtc tggcggggga ggtcgcggtg tccggacagg
    13261 acggcgacgc cgccggactg aaccgcgtgg cgctggggac gcagaccgtg gacgtctgga
    13321 aggacgctcg ggagctcggc aaggccgcgg gcgagtcggc gtgcgcgatg gcccagggca
    13381 cggcgctcca ggacgtcgcg aacacggagg cgttcgactc gccgggaggg aactcgatga
    13441 cttcgatctt cctgacgccc caagcgatca cgcaggacaa cctcgacgtc gtgctcgacg
    13501 caggctggat ctcggaggga gacctgtgcc agggcgtcga tcccggcacg gtggaggtgt
    13561 gcggctaacg gctcgaggcc gaagcacgca tcgtgaaccg caggcgctcg cctcacccga
    13621 cagggtgagg cgagcgcccc tcttggcata ggattctcac cggtggctga gaccaaggtt
    13681 tccgcggccg aacgagagcc cgaggcgcag gcggcgccgc ccgaggggca agggaaacgg
    13741 tcgctccgcg atctgttccg cgcggccgag gtcgatagtc ggctcgtcgg gttgctcggc
    13801 ggcctggcca tcatctggat catcttcgcc gtcatcacgc cgaatcacac gttcctcacg
    13861 ccccggaacc tgtggaacct gtcggtccag accgcggtcg tggcgatcat ggcgacgggg
    13921 atggtcctga tcatcgtcac ccgcaacatc gacctgtccg tcggtgccgt gatggccgcc
    13981 gttggcatgg ccatggccgt cgtccagcac gactggctgc ccgaatggat cgggtttgat
    14041 cacccgctca cctgggtgat cgccgtcgcg gtcggcctgg cgctcggcgc ggcgatcgga
    14101 gcgttccagg ggacgatcat cgcgtacgtc ggcgtgccgt cgttcatcgt gaccctcggc
    14161 gggttcctcg tgtggcgagg tgtcgcctgg tggatggcca gcggccagac gatcgcgccg
    14221 atggacacgc tcttccgcaa gctggggggc ggctccacgg ggaccgtggg cggcacgtgg
    14281 agctggatcc tcggcatcgc ggcgtgcgtg ggggtcgtgc tcctgcagtt caccaagcgg
    14341 cgccagcggg accggtacgg gttccggctg cgcccgatgt gggcggaggt cggaatcgtc
    14401 gtcatcgtgt gcgccgcgat cctcgtgtcg gtggggttcc tgaaccgtta ctacctgccg
    14461 ccggcgctgg ccgcgcaggc cggcaaggcc gggcagaaca tctcgctcgg cctggcctac
    14521 ccggtgctta tcgctatcgg cgtcggcatc gtgatgacgt tcatcgcgac tcggcggcgc
    14581 ttcggccgct acgtctacgg gatcggcggg aaccccgagg cggccgagct cgccggcatc
    14641 aagaccaggg ggacgatcgt caagacgttc accctgatgg gcttgctggt cgggctggcc
    14701 gccgcggtcc agatcgcccg gctcaacgcg gcggtgagca gcctcgggac cgggatggag
    14761 ctcaacgtga tcgccgccgc cgtgataggc gggacgtcgt tcagcggtgg tatcgggacg
    14821 atcgtcggcg cgctgcttgg cgcgctcgtc atgcagtcgc tagtgtcggg catggtgctc
    14881 atgggcatcg aggcgccgct gcaggacatc gtggtcggca tcgttctcgt gctggccgtg
    14941 acgctcgaca cgatcgtgcg caggagggct gcccggtgac cgaccgcacc ggcacgccgc
    15001 tcgtcgagct gcgcgacatc cgggtcgcgt tcggcggcgt ccacgccgtg gacggcgtga
    15061 cggtcgacct gtatcccggc gaggtggttg ggctggtcgg cggcaacggc gccggaaaga
    15121 ccaccctgat ccgaacgctg tctggggcgc acccggctga ctcgggccag atcctgatcg
    15181 agggcgagcc cgtgacgatc tcgcatccgc gggacgcgaa ggcgctcggg gtcgagacca
    15241 tctaccaggc cctcgcactg gcggacaaca tcgacgcgcc gggcaacgtg tttctcggcc
    15301 gcgagctcac gacgcggttc ggcaacctgg atgacagcgc gatggaggac gccacccgaa
    15361 agctcatggg gcggctcaac ccgaagttcc agaacttcgt cgtgccggtc gcctcgctct
    15421 ccggtgggca gcggcagtcg gtcgcgatcg cgcgggccgt ccacttcaac gcgcgcatct
    15481 tgatcatgga cgagccgacc gccgcgctcg ggccggccga gaccaggcag gtcggcgacc
    15541 tcatcaagca gctgcgggag gaagggctcg ggatcttcct gatcagccac gacatccacg
    15601 acgtgtacga cctgtccgac cggatcagcg tgatgctcca ggggaagctg gtcgggacgg
    15661 tgaacaagga cgacgtcacg acggacgagg tcctcgccat gatcatcctg ggcaagctgc
    15721 ccggcgaggt gacgcagaag gatctggccg aactgcactg acccccgcta tgggttcggc
    15781 ccgacttcca cgagcagcca ccgatccccg gtcggccggc tgagcacgaa agtcttcgag
    15841 aacggggaag acgatcggct ccgctcgctc ccgtctacgc ggtaggtgac ctcctcgacg
    15901 gttccgcgag cctggaagcc caggctcagg cccgcctggc cttcgggttg gacgagaacg
    15961 acgtggagct catcgaacgc gtagtggggt acgacggcgc gtccggtctc gatcacctcg
    16021 tcgacgcgtc gctcgagcgt tgtctgccgt tgcccgtcgt ccaccatgga caggaggctc
    16081 ttctctcccc gccggagcgc ctgggcctcg acctcgaggt tctcggccag cgtgagggcg
    16141 atcgcctggg cgtcggatcg cgtcaggtca ccgcggtcga cgacctcggg atcgacgctg
    16201 acccgcggga gcgtggaggg atcgacgatg accgaaagct cggggacctc cgatgcgctg
    16261 gacggcaccg acgcgctcgc agagacgtag gattcccgag ccggcgcccc cgccgcgacg
    16321 accgaggtgc cgacgagcac gatcgccgca ccggcgaccg ccccgcgggc gagcgcgcga
    16381 ccgggcgcga gatacccctc gcctcgccgg cccaacccga cgaggtaggc ggccggccgg
    16441 tcgcgttggg atccgggggc cggcagcagc ttctcgaaga agagccgcgc cacacatagc
    16501 acgaccaggc ccgacagcag cgccaccttg gcgccgaact cggtcgtctg cggcgcgatc
    16561 aacaaggtgc acaccatcgc gacgcagatc ccgaacacga cccgcgcgac ccgcccgtcc
    16621 ggcgtcgtct tcgggtcggt gatcatgaag aacaggaaga tcaggatctc gggcgagagc
    16681 acgatcaccc accagaagtg cgccccgcac acgggctcga acgaccaccg cgcggtcatg
    16741 caatggcccg aggcggccaa cacgcccatg ccgaccgcga gcgtgagcca gaacgccgcc
    16801 gacatcccca gcaggtgaag gcgagccgtg atgagcaggc ctcccacgag gatgatcagg
    16861 taggccgcga tcatccagcc gtcgaacggc gcccaccaga agtcgagcgg ttcgatccgg
    16921 gcgctgccga gcagcaggaa ggccgcgacg agaccaacgt tcgacgggtt gaagatgtgc
    16981 gaaccgcggt agcggatcag atacttcgtg agcagcgaca gccccgccac gagcgcgaac
    17041 acgtaccagc cccgccagct ccagtggtcg ttgctctgca tgtcgctcag gcgcaggatc
    17101 agcgcgacgc cgctgccggt cagcatcgcg ctcgccggcc agaccagtgt gccgatcttg
    17161 gagagggtcc acccgacctc gatgacggcg cagaccagga tcgccaccag gatctgcggg
    17221 accgacacgc ggaacccgag cccgatctgg cccaggacat ggatcgagat gatcaccgcc
    17281 gcgaggtgga gccgcgggtc gcggatgttc ggcgcgacga acgggtaggt gcgcccgagc
    17341 atccgaaccg tgcggccggc cggctccgcg ggcggctcgg gcgccgcgcg tcccggcgcc
    17401 acctccggcg tcgccggggc gtccgtcacc gaccgtgcag acgtctgcgc gatcggtgga
    17461 tgcggctcgt tcagggcgtc ccccagcacg ggttcgacag ggaggggtat cgtacgcgct
    17521 cgcccgcatc cgtccgacca cgagcgagga gagcagatga tcccgaccga gcccatcggc
    17581 agcatcccca gaccgaagaa cctgatcgac ggacaggcgg cgttcgcgga gggaaccatc
    17641 tctcccgagg agctccgcgc gctgcaggac gaggcgctcg cggagacgat cgagcgcttc
    17701 gaggagacgg gctcgcccgt gatctcggac ggcgagcaga ccaagtcgag cttcgtgacc
    17761 tatccgctcg acggcgtgac gaacctcgcg gccgacggcg tcgtgatccc gttcgcggac
    17821 ggccacacgc ggcagctgcc gaccctcgcg tcgggaccgt tccggttcgg cacctacgcg
    17881 gcgagctacc tgctggaggc gaagaggcgc gcgcacgtgc cggtcaagca ggccgtcatc
    17941 gccgcctccg ccctcagcct cctgtacccg caggacggga tcgaggggta ctcccgggac
    18001 gcgttcctgg acgacctcgt cggcgagagt gagaaggaca tccgcgcgtc cctcgacgcc
    18061 ggcgcccaca aggtccagat cgacttcacc gagggccggc tcgcgtgcaa gctcgatccg
    18121 tcgggcgggc tcctccggga cttcgtggcg ctcaacaatc gcgtcctcga gcgcttctcc
    18181 gacgacgagc gttcccggat cggcgtgcac acgtgcccgg gcggggacat ggactcgacg
    18241 cacagcgccg acgtcgacta cgcccagctg ctgccggacc tgttccagct caacgtcggc
    18301 aacgtctacg tcgagatggc gggcgagaag gaccgggccc gcgtcctcga catcatcgcg
    18361 gagcacctgg gccccgaaca gcggatcttc gtcggggtga tcgacccgat cgacccgagt
    18421 gtggagtcgc gggaggaggt gcgcgaccgg gtcctcgagg ccgccgagcg cctaccggcg
    18481 gaccggctcg gcacgaccga cgactgcggc ttcgcgccct tcgcggacga cacgtcgacc
    18541 tcgcgcgacg tggcgttcga aaagatccgc gctcgcatcg aggggacgcg cctggccggg
    18601 gagcgcctgg ggatctaggg gcggcgctcc gcacggcgaa ccagctgctt gacgcgtgcg
    18661 ggcgtcagcc tccgcgcgcc ggctccctcg agccacccga acacctcggt ccgcatcccc
    18721 agcgcggtca cgccgatcac gtcgccgcgc ccggaacgcg cgaggacgcc ctgcagcccg
    18781 gcgagctcat gggcgtagac gtcgacctcg accgcgccgc cgtccttcgc gccggcgatc
    18841 agccgatcga tcgtgctttg gcgctcgcgt ccccgcaggt accgcagcag ctcggcgatc
    18901 gcgaggtggt cggagccgcg cgccgctcgg tacccgaagt cgtgcaagat ctggtcggtc
    18961 cggtcgcccg ccgcgcagat cacgagccag atctcggcgc cggcgcgccg gaggccgtcg
    19021 cagatctcgg cgaaccccat catcccggcc tcgttgtgcg cgtagtcgac gacgatcact
    19081 cgcccgtcga gctcgaacag gttcgcccgg cccgggttgc gttcgggatc ggtgacgaag
    19141 gtcttgaggc cctttacgac cgcgcgttcg ggcaacccga tcgcgagggc cgcggaggcc
    19201 gcctgcatcg cgttcatcac gttgtggcgc gagacgcccg cgatcgtgac gggcacgtcg
    19261 atcagctcga ccagcggtcg tgacgacgag ccgtcgagga cggtcatcca cccgtccaga
    19321 ggcacggtcg cgcggccgcc gtcggccagg gtgtcgcgga tcgccgggtg ccagggatcg
    19381 agcgaacaga gccacggccg gcccgtcgcg gcccgcctca tcgacaggac ccgcgggtcg
    19441 tccgcattca gcacgtccca tccgtccgcc cgggtgatcc gcgtgatcgt ggccttcacc
    19501 tccgagagct ggtcgatcgt ctggatcccg tggagaccca ggtggtcctc ggacacgttc
    19561 gtcacgacgg cgacgtcgtt gtgcatcaca ccgatcccgc ggagcaggat gccgccgcgc
    19621 gccgtctcca ggacggcgac atccggtgct tcggcgagcg cgcgggccgc gccgccgaat
    19681 cccgagtagt cgccctcctc gatcagctca ccgtcgcctc gatacacacc gtccgtggac
    19741 gagtacgcca cggtcctgcc cgccgatcgg acgaggtgtg cgaggagccg gaccgtggtg
    19801 gtcttcccgt tcgtgcccgt gaccgagacc accgggatct cgggctcggg gacgtcgggc
    19861 tcgttgcccg gttcggccgc cgcgacccgt tcggccgcct cggcgaggag ccggtcgacc
    19921 gaacggcgac cgcctacctc gtgcaggagc ggcgccacct cgcgaccgag cgcctcggcc
    19981 gcgccgcgcc gccgccacgg gaacgcgacg acgacctggt cgcgttcggg tcccgggcgg
    20041 ctgcggaccg cgagacgcac ccgactcgcc gtcgcgagcc gccacgtgac tcgagcggcc
    20101 aggcgcgaga tcgtccgtct gcgttggtcg gaacccggcc ggccggggcg tccggtcgcg
    20161 cccacgcgtt cgagcgcggt ctccagccgt tcctccggga cgtcgagcca gcccccgacg
    20221 gcgatcgtga gcttgaccgc ggggcgtggg aagtacagat tcggaccgtc gagcacccgg
    20281 agctcgacga ggtcggcgcg cggtggggcc acgggcgcga gcttagtcag ccggggtcgc
    20341 cggcgtggta ccggcggaac gcggggacgc gccacgcgac gagcccggcg ccggcgatgc
    20401 agagcagccc gcccgagacg accgacacca acggagagaa ggccgcggcg acgaggcccg
    20461 cctccaggtc gccgagcctc ggcccgccgg tcaccaccag gatgtggatc ccgaacagcc
    20521 gcccgcgcag gttctcgggg acggtgacct gcaggatcgt gttgcggaac accgccgaga
    20581 tcacgtcggc cgccccggcg atcgccagga atccgaggcc gagccacagg tgcgacccca
    20641 ccagcccgaa cgccgcgatt ccggcgcccc acacgacgac cgcccagatc accgccagcc
    20701 cctgatgacg gatccggccg acccagccgc tcgtcgccgc gcccacgaac gcacccacgg
    20761 cgggcgccgc gaacaggaac ccgacgaccg tggcgccccg atggaactgc gtgagcgcga
    20821 gcacggggaa cagcgcccgg ggcatcccga acaccatcgc gatgatgtcg atcacgaacg
    20881 tcgactgcag cacctggcgg ccgcgcagga acgcgaaacc ctcccgcacc gcgcgccagc
    20941 ccaccgccgt gcgttcgccc tcggcgggcg gcatcggccg gatcgagatg gaggcgagca
    21001 gcagcaggcc gatcgagatc aagtcgatgg cgtatgccca cgagaacccg aaacccgcga
    21061 cgacgatccc cgcgagcgcg ggtccgacca gaccggcggc gttccagatg atctggttga
    21121 gcgcagctgc ggccggcaac tggctgcgat ccaacaatcg cggcgtcatc gcgctgcgcg
    21181 tggggccgtc gatcgcactc gtcgcggcga tgcctgccac ggccacgtag atcagcgcga
    21241 gcggcgggtc gccgtgcacc gctcccgcga acagcaggcc ggaggacgcc aggtacccga
    21301 tctgagccga gatgaggatc ttccgccgat cgaacgcgtc caggaacgtc ccgcccgcca
    21361 gcgtccccgc cacgagcgcg aggaagccga cgagcccgat cagcccgacg gcggccgtcg
    21421 agcgggtcag cccgtacacc tggaggaacg tcgcgacgat cgtgaactgg gaaccgaagg
    21481 tcgacgccag caggccgagc cacagccgcc ggaaatcgcg cgacgctcgg agcggagtga
    21541 cgtcgaccgc gatccgccgg acgagcgagc gcttgctctc gtcgttcggc atgtcccgga
    21601 cgctagcagg ccggccgcga gacaaagacg tgtccgaggc gaacgttcaa tcgcccgggc
    21661 gacggaccgg aggtgccagc tcggtagccc gcccggcgcg atagagcctc gccgggcggc
    21721 cgccactccg cgtcgtcgtc gcctgggtcg gaacgacgaa accggcgctc gacaggacct
    21781 tgcgcgagaa gttccgtggg tccagcgatt cgtcccaaat ggcctcgtag acctcacgca
    21841 gttcggcgat cgtgaactcg ggggggcaga aggaggtggc gagggtcgtg tactcgagct
    21901 tgccgcgcgc tcgctcgatc ccgtcttcca ggatcctgtc gtggtcgaaa gcgagctggt
    21961 tcactgcgag atcacgaacc cgcttccacc ccgccgtagc ggcatcggtg ccggcggtgg
    22021 gggagggcgg ctcgggcacc attgccagat aggccacgct caccacccgc attcgggggt
    22081 cgcggtccgg atctccgtac gtagcgagct gctcgagatg ggcgacccgg atacccgtct
    22141 cctctgcaag ttcgcgcgcg gccgcctcgt ccagcccctc atcgacgtgg acgaacccgc
    22201 cgggaagcgc tagctcgccg gcgaagggct cctctccccg ctcgatgagc agggcttgga
    22261 aagcgccatc gatcaccgtg agcacgacga ggtcgacggc gaccccgaaa ggcgggtgag
    22321 ctctggttcg tgtcatcttg acaggaagtc tccggagggg ttatcgtcac tgtgacgata
    22381 acggaccgag agagcggagg caaggatgac cgccagcgac ctcctcgaat ggatcggatt
    22441 cctctccggt gtgctgtgcg tctggtgggt ggtcaaggag aggatcgcca actggccgat
    22501 gggcatcgtg aactgcctcg cctggctgat cctgctctgg atcaacgcga tctacctcaa
    22561 cgcgctcctg cagctcgtgt acatcgtgat cagcgtctac ggctggtggc actggttgtg
    22621 gggaggcgag gctcgcgact cgctgccggt gcgttacgcc acgtccgcag agcgccttcg
    22681 agccgccgcg gtgatcgccg tcggaggcgt cgccggtgcc cgcctgatgg tgcttgccgg
    22741 agatgcggcg ccgatctggg atgccctcac gacgatgacg agcatcgtcg cgatctactg
    22801 gcaggcccgg aagatctacg agagctggat cctgtggttg agcgtcgaca tcatctactt
    22861 cgcgctctac ggctcccagg acctgtggct cacggcgcta acgcaggtgg cgttcggcgc
    22921 gatgtgcatg gtcggcctgc gtgattggcg acgctcgatg caacgagagg ccgtggcggc
    22981 atgacgcacg gggtcgtggt cggcaagttc tacccaccgc atcgcgggca ccggcatctg
    23041 atcgacgtgg ccgtctcgca gagtgaccga gtgaccgtgc tcgtcgtgga cggccgggac
    23101 gaggatccgc cggctgaagc acgggccggg tggcttcgcg agctccatcc ggacgcgacg
    23161 gtgaaagtca ctcgggacat cgagcgcgac gaggactccg tggcgtgggc gcgcgcaaca
    23221 cgccaagcgg tgggcgggca gacgatcgac agcgtcttca actcggaacc gtacggctcc
    23281 gcgtgggcgg ccgcgatctc ggaccagcag gggagcgact gcgagaacgt gttcgtcggt
    23341 cgcggattcg tccagatctc ggcgacgcag gttcggtcgg acccggctca ctactgggac
    23401 tacatcgatg caccggtgcg cgctcactac gtcaagcgtg tctgcatcgt cggtgccgag
    23461 tcgacagggt cgaccaccct cgcgcggtcg ctcgcggcag cctgcgagac catctgggtc
    23521 cccgaatacg gccgcacgta cacggtcgag aagttcagga tcggaacggg ggacaggtgg
    23581 ctaccgagcg acttcctccg catcgctctc gaacagaatg ccctcgagga tcggatggct
    23641 cgggaggctc cggtaggcat catgttctgc gatacggact cgctcgcgac ggcgctctgg
    23701 gaggaggtct acctcgggtc gacctcggat gccgtctggc gcgctgccga tgcccacggc
    23761 gccccgtacg tcctgtacct gctcactgac cacgcgggca tcccctggga ggacgatggt
    23821 ctgcgtctcg gggacgagac acgcgcagcg atgacccaaa ggttcgagga ggtgctgagc
    23881 gctcggggcc tcccgtggat gaaggtctct ggttcccaga aggaaaggct tcatcaagcg
    23941 gttgaaggaa tccgttccag tgtggagcac gacttcacgt tcggccccgt cgatcttcat
    24001 gaccgtgagg cgatcaatct ctgagcctag gcgccgatcg ccgcgtccag ggtctcgagc
    24061 ccctcgtcca acagcccttc ctcgatcgtg atgggcggca gcaaccggat cacgttgtcg
    24121 taggtgcccg ccttcaggat cacgacgcct tcctcgaggc agcgtctgac gatcgcgttc
    24181 ccatccggac cgcttagcgg ctctttcgtc gcgcggtcgg agacgagctc gatcgccgtc
    24241 atcatcccgc gcccccggac gtcgccgatc aactggtggc ggtcctgcat ctcgcgcaac
    24301 cggccgagca ccctctcgcc gagggtgcgt gagcgctcga gcaggttctc cttctcgatc
    24361 agatcgagaa ccgcgagcgc cgccgcggcc gcgaccgggt tgccgccgta cgtgccgccg
    24421 agcccgccga cgtgcaccga gtccatgacg tcggcgcgac cggtgatggc gctgatcggc
    24481 aggccgccac cgagcgactt cgcgctggtg atcaggtcgg ggacgacgcc ctcgtcctcg
    24541 atcgcgaacc agcggccggc gcggcccatg ccgctctgga tctcgtcggc gatgaagagg
    24601 atcccgtgct ccgagcagaa ctctgcgagg cccttgacga agcccggcgc cggcacgatg
    24661 aagccaccct cgccctggat cggctcgagg atcacggccg cgacgttgtc ctcgccgatg
    24721 tgcttgtgga tctcgtcgag cgcgtagtcg agcgcctcct cggcgcatcg ctccggaccg
    24781 cccggccacc tatacgggta ggcgaacggg agccggtaga tctcgggcgc gaacggcccc
    24841 atgccctgct tgtacggcat gaccttggcg gtgagcgaca tcgcgagcag cgtccggccg
    24901 tggaacgcgt ggtcgaacgt cacgatcgcc tggcgccccg tcgcctttcg cgcgatcttc
    24961 accgcgttct cgacggcctc ggcgcccgag ttcgcgaaca tcgtcttctt cggccc
//
LOCUS       DATBRK010000177         6468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2049899, whole genome shotgun sequence.
ACCESSION   DATBRK010000177 DATBRK010000000
VERSION     DATBRK010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049899"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VIH70_05345"
     CDS             complement(<1..685)
                     /locus_tag="VIH70_05345"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY8115820.1"
                     /translation="MAYRIRPELSRPVMVCAFGGWNDGGEAATTAVRTLRTQWGARRF
                     AEIDPEEFYDFQVHRPTVRLVEGSTRRVDWPASRFFSARINDRDAIVFVGVEPNTRWR
                     TYAGTILQVCDDLDVSLLVTLGAFLADVPHTRPAPVNAASEDEDWLAKPGIVPARYEG
                     PTGIVGVLNSAASNAGLPALSLWAAAPHYLPQSRNPKVALALLEALRDLTGLEIDTGE
                     IELAARVFER"
     gene            770..2587
                     /gene="dacB"
                     /locus_tag="VIH70_05350"
     CDS             770..2587
                     /gene="dacB"
                     /locus_tag="VIH70_05350"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY8115821.1"
                     /translation="MLVRDDGWWHDALLARRGETAIGARSTFGRRLRRFAFIELALLS
                     TLLLPSALAETATATLSASAPIVNFGEAVTVSGSIAADPGCIGGRAVTLQWQPVDSTA
                     FSNVATGATAADGSFTFVQTQPHTGRYAATIEQDASCLASTTNQVLVRVRAMVDAALV
                     AGPSGSCVDISMSVMPERPGQFVELERRTQQGWTLIERLTLDPASQALASPCFASGEV
                     GVVRLRVRWVAQDTLNETGSSPVLAIELSKARWMLEIDHAIGTRRVSVAVGEDDTLLY
                     DRAASALRIPASNEKLLLSMTLYETLGSDFRIRTSVASSGGSSGAVRNLWILGRGDPG
                     VTGATIGTLARRVADAGIERVRGRVFGSTGYFRRDWDAPGWNAEARDSVNRPTALVFE
                     RNVGPNPEREAAEMLTRKLEALGVRVRGRSGTGRPPGGLETIASVTSQPLQRLLTKML
                     RPSDNFIAEMLGKRLGVETRGVPGTIAKGAAAIEAWTDDHGTGFTLNDSSGLSYANRV
                     TAEGIVRLLWFAEDQPWGRDLRRAMPTGGQGTLSHRLRGVDVRAKTGTLDGVSALSGW
                     VMDRSGDWVEFSVLSFGMSKSTASAIEDRIVVILHDHLA"
     gene            2664..3842
                     /locus_tag="VIH70_05355"
     CDS             2664..3842
                     /locus_tag="VIH70_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8115822.1"
                     /translation="MDPTTAAPGAAELLELGLARDGHEVGVSYEALVEHHAETEAKYR
                     NLVERLPCVVYLAQYGPNGEWLYVSPQIEQVLGYSQKEWLEHPHPQGSFTHPDDLPRL
                     NAEESRSMASGDPLDVEYRIRRADGAWVWIHDEATAVLDEDGNPICLQGMMFDITKRR
                     AEQERLISLDRLKNTLLHTLSHDLKAPLTAILVAASTLERLGDELDDEVRQHMLRTLV
                     ERSKGMNALLTDLLDLDRLDQGIVAPRRYPLDLHELIRHLLDKTKVLGDRTVELEAGE
                     CRANVDQPKVERILENLLANADLHTPVGTRIWTRCWREGDAAVIAVEDDGPGVPSELR
                     DKIFETFYRGPDSVHKPGSGIGLSLVARFAEMHGGRAWVQARDGGGSSFRVLLPDRSS
                     "
     gene            3880..5259
                     /gene="leuC"
                     /locus_tag="VIH70_05360"
     CDS             3880..5259
                     /gene="leuC"
                     /locus_tag="VIH70_05360"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY8115823.1"
                     /translation="MRTPSTLAEKVWERHVVHRADGEPDLLYVDLHLVHEVTSPQAFE
                     GLRLAGRTVRRPDLTVATMDHNVPTAAGPVDPVSRTQMEALERNCTQAGITLFPTGDL
                     GQGIVHVIGPQMGLTQPGLMIVCGDSHTSTHGAFGALAIGIGTSEVEHVLATQTLPQR
                     TPKTMAVTVEGELPDGVSAKDVILGIIAMIGTDGGVGHVVEYRGSAIRSLSMEGRMTV
                     CNMSIEAGARAGMVAPDETTFAYLEGRPNAPTGSRWDEALEDWRSLPTENGATFDKEV
                     ALDATDLRPHVSWGTNPGQTVTIDDVVPEPADEAAERALAYMDLRPGTPIRDIRPDTI
                     FIGSCTNSRIEDLREAARVLDGRKVREGLRAMVVPGSMLVKRQAEREGLDTVFAAAGF
                     EWRDAGCSMCLGMNPDQLSPGERCASTSNRNFEGRQGKGGRTHLVSPAVAAATAVLGR
                     FAAPEELDA"
     gene            5256..5849
                     /gene="leuD"
                     /locus_tag="VIH70_05365"
     CDS             5256..5849
                     /gene="leuD"
                     /locus_tag="VIH70_05365"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY8115824.1"
                     /translation="MKPFTEHTGRALPLDRSDVDTDQIVPARYLRRIERSGFGPFLFA
                     EWRKDPDFVLNRPEFAGATILLAGPNFGSGSSREHAVWALEDAGFRTVIAPSFADIFR
                     TNCSKIGVLPVELAQESVRALMDAVLDDPTTEITVDLQALRVSAPAVHEAFEIDPYTR
                     WRMLEGLDDIDLTLRNADAIDAYERARPARLPSVTRP"
     gene            5919..>6468
                     /locus_tag="VIH70_05370"
     CDS             5919..>6468
                     /locus_tag="VIH70_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEY8115825.1"
                     /translation="MSKPEHLDAADDGRPRTGLPRRRAGDVLVPRPLADKPLRRESDG
                     WLERWMATVEDLARLKSSHPMLDAVIDEQVGRRIRIGEHWLADFASCNYLGLDLDQEV
                     IDAVPEYLAKWGTHPSWSRLLGSPVLYEQLEATTTALVGSEDSLLLPTITHIHMSVIP
                     VLAGSGVIFLDGRAHKTIYDGAS"
BASE COUNT          981 a         2284 c         2276 g          927 t
ORIGIN      
        1 cccgctcgaa cacccgggcg gcgagctcga tctcccccgt gtcgatctcg agcccggtca
       61 ggtcgcgaag cgcctcgagc agcgcgagcg cgaccttcgg gttcctcgac tgcggcaggt
      121 agtggggtgc ggccgcccac aacgagagcg ccggcaggcc ggcgttcgac gcggccgagt
      181 tcaggacgcc cacgatgccc gtcggcccct cgtaccgggc cggcacgatc cccggcttgg
      241 ccagccagtc ttcgtcctcg ctcgcagcgt tcacgggcgc cggccgcgtg tgcgggacgt
      301 ccgcgaggaa ggccccgagc gtcacgagca gggacacgtc caggtcgtcg cagacctgca
      361 ggatcgtccc cgcgtaggtg cgccagcgcg tgttcggctc cacgcccacg aacacgatcg
      421 catcgcgatc gttgatccgc gccgagaaga agcggctggc cggccagtcg accctccgcg
      481 tcgagccctc gaccagtcga accgtcggac ggtggacctg gaagtcgtag aactcctcgg
      541 gatcgatctc cgcgaagcga cgggcgcccc actgcgttcg gagcgtgcgg accgccgtgg
      601 tcgcggcctc tccgccgtcg ttccagccgc cgaacgcaca caccatcacg gggcgcgaga
      661 gctccgggcg gatccggtac gccacgtggg ccatccgcac agtgtgtcgt ctcgaggggg
      721 tgcctgtcat ccctgccccc gacccgatcg aggcgccgct tcgagcgggg tgcttgtccg
      781 cgacgacggc tggtggcacg atgccctgct cgcacgcagg ggggaaaccg cgatcggagc
      841 ccgctccacg ttcggccggc gactgcgcag gttcgcgttc atcgagcttg ccctgctctc
      901 gacgctgctc ctgccctccg cgctcgccga gaccgccacg gccacgctct ccgcgtcggc
      961 cccgatcgtg aacttcggcg aggcggtcac ggtctcgggt tcgatcgccg ccgaccccgg
     1021 gtgcatcggt ggccgggccg tgaccctgca gtggcagccg gtcgactcca ccgcgttctc
     1081 gaacgtcgcg accggggcga cggcggccga cggctcgttc acgttcgtgc agacgcagcc
     1141 gcacacgggc cgctatgccg cgacgatcga gcaggatgcg tcgtgcctag cctcgaccac
     1201 caaccaggtg ctcgtacgcg tccgcgcgat ggtggacgcc gcgctcgtcg ccgggccgtc
     1261 gggctcgtgc gtcgacatct cgatgtcggt tatgccggag cgacccgggc agttcgtcga
     1321 gctggaacgt cggacgcagc agggctggac gctgatcgaa cggctcacgc tcgacccggc
     1381 gtcgcaggcg ctggcgtctc cgtgcttcgc ctccggggag gtcggcgtgg tgcggctccg
     1441 cgtgcgctgg gttgcgcagg acacgctcaa cgagacgggc agttcgcccg tccttgccat
     1501 cgagctctcc aaggcccggt ggatgctcga gatcgaccac gcgatcggga cgcgacgcgt
     1561 ctccgtcgcc gtgggtgagg acgacacgtt gctgtacgac cgggcggcct ccgcgctccg
     1621 gatcccggcg tcgaacgaga agctcctgct gtcgatgacc ctctacgaga ccctcgggtc
     1681 cgatttccgg atccggacca gcgtcgcttc gtcgggtggc tcgtcgggag ccgtccgcaa
     1741 cctctggatc ctcgggcggg gcgaccccgg cgtgaccggg gcgacgatcg gcacgctcgc
     1801 gcgcagggtg gccgacgcgg ggatcgagcg ggtgcggggc cgcgtgttcg ggagcacggg
     1861 ctacttccga cgcgactggg acgcgccggg ttggaacgcg gaggcgcgcg actccgtgaa
     1921 ccgcccgacc gcgctcgtgt tcgaacgcaa tgtcggaccc aacccggagc gggaagcggc
     1981 ggagatgctc acccgaaagc tcgaggcgct cggcgtccgc gtgcggggca ggtccggaac
     2041 cgggaggccg ccgggcgggc tcgagacgat cgcgtccgtc acctcgcagc cgctgcagcg
     2101 gctgctcacc aagatgcttc ggccctcgga caacttcatc gccgagatgc tcggcaagcg
     2161 gctcggcgtc gagactcggg gcgtgcccgg gacgatcgcg aagggcgccg ccgcgatcga
     2221 ggcctggacg gacgatcacg ggacggggtt cacgctgaac gatagctcag gcctctcgta
     2281 cgcgaaccgc gtgacggccg aggggatcgt ccgcctgctg tggttcgccg aggaccagcc
     2341 gtggggacgg gatctgcgtc gtgcgatgcc gacgggcggc caggggaccc tcagtcatcg
     2401 cctgcggggg gtcgacgtcc gggccaagac cgggacgctg gacggcgtgt cggcgctgtc
     2461 ggggtgggtc atggaccgct cgggcgattg ggtggagttc tccgtgttgt ccttcggcat
     2521 gtcgaagtcg accgcctctg cgatcgagga ccggatcgtc gtgatcctgc acgatcacct
     2581 cgcctgacgc agccacccgg ccgtggatga gagaaagcct tccaccggtc aacccggagc
     2641 ggcttcccac cgatgctgag gagatggatc cgaccaccgc ggcacccggt gcggccgagc
     2701 tcctggaact cgggctcgcg cgcgatggac acgaggtcgg cgtcagctac gaggccctcg
     2761 tcgagcacca tgccgagacc gaggcgaagt accgcaacct cgtcgagcgg ctcccgtgcg
     2821 tggtctacct ggcccagtac gggccgaacg gcgaatggct gtacgtctcg ccccagatcg
     2881 aacaggtgct cgggtactca cagaaggagt ggctcgagca cccgcacccg caggggtcgt
     2941 tcacccaccc cgacgacctc ccgcgcctga acgccgagga gtcgcgctcg atggcgagcg
     3001 gcgatcctct cgacgtcgag taccggatcc gccgcgcgga cggggcgtgg gtgtggatcc
     3061 acgacgaggc cacggcggtg cttgatgagg acgggaaccc gatctgcctg caggggatga
     3121 tgttcgacat caccaagcgg cgtgccgagc aggaacgcct gatctcgctc gatcggctca
     3181 agaacaccct gctccacacc ctgtctcatg atctgaaggc gccgctcacg gcgatcctgg
     3241 tggcggcgag cacgctcgaa cggctcgggg acgagctcga cgacgaggtg cgccagcaca
     3301 tgcttcgaac gctcgtcgaa cgatccaagg ggatgaacgc gctgctcacc gacctgctcg
     3361 acctcgaccg gctcgaccag ggcatcgtgg cgccgcggcg gtacccgctg gacctgcacg
     3421 agctgatccg acacctgttg gacaagacga aggtgctggg cgaccgaacg gtcgagctcg
     3481 aggccgggga gtgccgggcc aacgtcgatc agccgaaggt cgagcgcatc ctcgagaacc
     3541 tcctcgcgaa cgccgacctg cacacgccgg tcgggacgcg gatctggacc cgctgttggc
     3601 gtgagggcga cgccgccgtg atcgcggtcg aggacgacgg ccctggggtc ccgtccgagc
     3661 tccgcgacaa gatcttcgag accttctacc gcggccccga ttcggtgcac aagcccgggt
     3721 ccgggatcgg cctgtcgctc gtcgcgcggt tcgccgagat gcacggcggt cgagcctggg
     3781 tgcaagcacg cgacggcggc ggctcctcgt tccgcgtcct gctccccgac cggagcagct
     3841 aggtcgggtg ccgcgggggc ggcctgtacc atcgaaacca tgcgcacgcc gagcacactg
     3901 gccgagaagg tctgggagcg tcacgtcgtc caccgggccg acggcgaacc cgacctcctg
     3961 tacgtcgacc tccacctcgt gcacgaggtg acgagcccgc aggccttcga ggggctgcgg
     4021 ctcgcgggtc gcaccgtccg gcgccccgac ctcaccgtcg cgacgatgga tcacaacgtc
     4081 ccgacggccg cggggcccgt ggacccggtg agccgcaccc agatggaggc gctcgaacgc
     4141 aactgcaccc aggccgggat cacgctgttc ccgaccgggg atctcgggca ggggatcgtc
     4201 cacgtgatcg gcccgcagat gggcctcacg caaccggggc tcatgatcgt gtgcggcgac
     4261 tcgcacacga gcacgcacgg cgcgttcggg gccctcgcga tcggtatcgg tacgagcgag
     4321 gtcgagcacg tcctggcgac gcagacgctg ccgcaacgaa cgccgaagac gatggcggtc
     4381 accgtcgagg gcgagctgcc ggacggggtg tcggccaagg acgtgatcct cgggatcatc
     4441 gccatgatcg ggaccgacgg gggcgtgggt cacgtcgtgg agtaccgcgg gtcggcgatc
     4501 cgctcgctct cgatggaggg ccggatgacg gtctgcaaca tgtcgatcga ggccggcgct
     4561 cgcgcgggca tggtcgctcc cgacgagacc accttcgcct acctcgaggg ccgcccgaac
     4621 gccccgaccg gctcgcggtg ggacgaggcc ctcgaggact ggcgatcgct gccgaccgaa
     4681 aacggagcca ccttcgacaa ggaggtggcg ctggacgcga cggacctgcg gccgcacgtg
     4741 tcgtggggaa ccaaccccgg acagacggtc acgatcgacg acgtcgtccc cgagcctgcc
     4801 gacgaggccg ccgagcgcgc cctcgcgtac atggacctgc gcccgggcac cccgatccgc
     4861 gacatccggc ccgacacgat cttcatcggc tcgtgcacca actccaggat cgaggatctc
     4921 cgcgaggcgg cgcgggtcct ggacggacgg aaggtccggg agggcctgcg cgcgatggtg
     4981 gtgccgggct cgatgctcgt gaagcgccag gccgaacgag aggggctcga cacggtcttc
     5041 gcggccgccg gtttcgagtg gcgcgacgcc ggctgctcga tgtgtctggg catgaacccg
     5101 gatcagctct cgccgggcga acggtgcgcg tccaccagca accggaactt cgagggtcgt
     5161 cagggcaagg gcggccggac ccacctggtc agcccggccg tggcggccgc gaccgcggtc
     5221 ctgggtcgct tcgcggcgcc ggaggaactc gacgcatgaa gcccttcacg gagcacaccg
     5281 gacgggcgtt gccgctcgat cgctcggacg tcgacacgga ccagatcgtg ccggcccggt
     5341 acctgcgccg gatcgagcgg tcggggttcg ggccgttcct gttcgccgag tggcgcaagg
     5401 acccggactt cgtcctgaac cgccccgagt tcgccggtgc gacgatcctg ctcgccggcc
     5461 cgaacttcgg gagcggctcc agccgcgagc acgccgtgtg ggcgctcgag gacgccgggt
     5521 tccggacggt gatcgcgccc tccttcgcgg acatcttccg taccaattgc tccaagatcg
     5581 gcgtcctgcc cgtcgagctc gcgcaggagt ccgtccgggc gctgatggac gcggtgctcg
     5641 acgaccccac gaccgagatc acggtcgacc tgcaggccct gcgggtgagc gcgccggccg
     5701 tgcacgaagc cttcgagatc gacccctaca cccgctggcg catgctcgag gggctcgacg
     5761 acatcgacct cacgctgcgc aacgctgacg cgatcgatgc ctacgagcgg gcccggcccg
     5821 cccggctgcc gtcggtcacc cggccctgac ccgctcggcg ggcgccctcg cccatcgctc
     5881 tcaagcgtct cttcgcaggg gtcgatgcca tcagggcgat gtccaagccc gagcacctcg
     5941 acgcggccga tgacggccgt ccccgaaccg gccttccccg gcgacgggca ggcgacgtcc
     6001 tggtcccacg acccctggcg gacaagccgc tccgacgcga gagcgacggg tggctggagc
     6061 ggtggatggc caccgtggag gatctggcgc gcctgaagtc gtcccacccg atgctcgacg
     6121 ccgtcatcga cgagcaggtg ggacgccgga tccggatcgg cgagcactgg ctcgcggact
     6181 tcgcctcatg caactacctg ggcctcgacc tcgaccagga ggtcatcgac gcggtccccg
     6241 agtacctcgc gaagtggggg acccacccgt cctggtcccg gctgctcggc agccccgtcc
     6301 tgtacgaaca gctcgaggcg accaccacgg cgctggtcgg gtccgaggac tcgctgctcc
     6361 ttccgacgat cacccacatc cacatgtccg tgatccccgt cctggccggc agcggcgtga
     6421 tcttcctcga cggccgagcg cacaagacga tctacgacgg cgcgagcg
//
LOCUS       DATBRK010000178         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2484381, whole genome shotgun sequence.
ACCESSION   DATBRK010000178 DATBRK010000000
VERSION     DATBRK010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484381"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1410)
                     /locus_tag="VIH70_05375"
     CDS             complement(28..1410)
                     /locus_tag="VIH70_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8115826.1"
                     /translation="MTRGLIESSQTRVLPLEFERAYPVVSRGDGVWVEDVSGDRYLDA
                     MSGGSMAATLGHGRSDIVAVARSQAERLAYVHNERLTNPAQERLAQELVDVAPEGFSR
                     VRFVTSGSDANETSIQLARSYHVERGEPSRWQVITFAQAYHGPTMETLALTGRPGLHG
                     PFGPYLPEHLHIPPSTSRDDPTGRSALDALDRVIERAGPDTVSAFFCEAISAAALPAS
                     TPSAGFWEGLAERRERHGFLVCFDEVVTGVGRTGQWFAGEGTACTPDIIATAKGLGAG
                     YAAIGAVLCREPVYEAIANGSRRFPLGHTWDGAPLSCAVGLAVLDVLRSEDLIPRVEE
                     RGRRLRDELEAALADVPIVQEVRGHGYLLGVSFADPRDGSFLPPEFRVAGRIDQAAFE
                     RGLITLSTQPTRDGFAGDQTLFAPPFTTTDEELAEMVSRFADAVHRVAQDVEPELSAS
                     PSPTTTGADR"
     gene            1630..>2248
                     /locus_tag="VIH70_05380"
     CDS             1630..>2248
                     /locus_tag="VIH70_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY8115827.1"
                     /translation="MESVGKVLDGWRKDGIAFVRFELPDMHGISRSKTIPIAHAADYA
                     ERGLNMYGGVSVLDSRSDVVPGTLYHEEVGYGDQLLFPDPESAAVIPWADRTARFICD
                     ARWYDGRPLTATPRHVFRNAVERAREMGFEPLMGSEFEFYLLTEDTHEPLFDGYQIFN
                     TVRNDFVPTIRRIVEEMPRVGVDVITANCEYAGSQWEINFAPGRGL"
BASE COUNT          325 a          801 c          794 g          328 t
ORIGIN      
        1 ggatcagcgc gtgcagggtc gtctcggtca tcggtcggct cccgtggtcg tcggcgatgg
       61 gctcgcgctg agttcgggtt cgacgtcctg cgcgacgcgg tgcacggcgt cggcgaaccg
      121 cgagaccatc tcggccagct cttcgtcggt cgtcgtgaac ggtggggcga acagggtctg
      181 gtcgcccgcg aagccgtcgc gcgtcggctg ggtcgagagc gtgatcagtc cgcgttcgaa
      241 cgccgcctgg tcgatccgcc ccgcgacccg gaactcgggc ggcaggaacg atccgtcccg
      301 cgggtcggcg aacgacaccc cgagcaggta cccgtgcccc cggacctcct gcacgatagg
      361 aacatcggcc agcgccgcct cgagctcgtc ccgaaggcgt cgcccgcgtt cctccacacg
      421 agggatcagg tcttcggagc gaaggacgtc gaggacggcg aggcccacgg cacacgagag
      481 cggcgctccg tcccaggtgt gacccagggg gaaccggcgc gagccgttcg cgatggcctc
      541 gtagacgggc tcgcggcaca gcaccgcgcc gatcgccgcg tacccggcgc cgaggccctt
      601 cgcggtcgcg atgatgtccg gcgtgcacgc cgtcccctcg ccggcgaacc attgacccgt
      661 tcgcccgacg cccgtgacca cctcgtcgaa gcagaccagg aacccgtggc gctcgcgccg
      721 ttcggccagg ccctcccaga agccggcgga cggcgtcgac gcgggcagcg cggcggcgct
      781 gatggcctcg cagaagaacg ccgagacggt gtccggaccc gctcgttcga tgacgcggtc
      841 caacgcgtcg agcgccgacc ggccggtcgg gtcgtcgcgc gacgtgctcg gggggatgtg
      901 gaggtgctcg gggaggtagg gtccgaacgg tccgtgcaac cccggccgcc cggtcagcgc
      961 gagcgtctcc atcgtcggtc cgtgataggc ctgcgcgaag gtgatcacct gccagcgcga
     1021 gggctcgccg cgttcgacgt ggtagctccg agcgagctgg atcgaggtct cgttcgcgtc
     1081 cgatccgctc gtgacgaacc gcacgcgcga gaacccctcg ggcgccacat ccacgagctc
     1141 ctgcgcgagt cgctcctgcg cggggttggt cagccgttcg ttgtgtacgt aggcgagccg
     1201 ctcggcctgg gatcgggcca cggcgacgat gtcgctccgc ccgtgcccga gcgtcgccgc
     1261 catcgagccc ccgctcatcg cgtccaggta gcggtcgccc gacacgtctt ccacccagac
     1321 gccgtcgccc cgcgacacga ccgggtaggc ccgctcgaac tcgaggggga gcacgcgcgt
     1381 ctgactcgac tcgatcaggc cccgcgtcac gcgccctcct cgccggtgga cgtgcgacct
     1441 gccccgccga ggccgggccg ccgcacgtcc gaagcgtacc gcgcggccgc tctgccaggg
     1501 gaccctcccg tcgctccccc cgaacacgtt acgatcgagc gatcggccgg ctcgccgccc
     1561 ccacgggatg gttgggaagg cctgcgggcg aaggcgcatg ggccgttggg gcccggaagg
     1621 aggagccggg tggagagcgt cgggaaggtc ctcgatggat ggcggaagga cgggatcgcg
     1681 ttcgtccggt tcgagcttcc cgacatgcac gggatctcgc gttcgaagac gatcccgatc
     1741 gcccacgccg ccgactacgc cgagcgaggt ctcaacatgt acggcggggt cagcgtcctc
     1801 gattcgcggt ccgacgtcgt cccgggaacc ctgtaccacg aggaggtcgg gtacggcgac
     1861 cagctcctgt tccccgatcc cgagagcgcc gccgtgatcc cgtgggccga tcgcaccgcg
     1921 cgcttcatct gcgacgcgcg gtggtacgac ggacggccgc tcaccgcgac gccccgtcac
     1981 gtcttccgta acgccgtcga gcgggctcgt gagatggggt tcgagccgct gatgggctcg
     2041 gagttcgagt tctatctgct gaccgaggac acgcacgagc cgctgttcga cggctaccag
     2101 atcttcaaca cggtccgcaa cgacttcgtc ccgacgatcc ggcggatcgt cgaggagatg
     2161 ccgagggtcg gcgtcgacgt catcaccgcc aactgcgagt acgccgggtc gcagtgggag
     2221 atcaacttcg cgcccggacg tggcctgc
//
LOCUS       DATBRK010000179         9436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1761362, whole genome shotgun sequence.
ACCESSION   DATBRK010000179 DATBRK010000000
VERSION     DATBRK010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761362"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VIH70_05385"
     CDS             complement(<1..338)
                     /locus_tag="VIH70_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEY8115828.1"
                     /translation="MAGERRTIVFMPESAYGPTNNCIGIGKVLERRGHRVVFAAEASW
                     KGKLEPLGFEEDLVDLSPPAEGDQDAGQFWIDFVAETAPEFRKPTIEQLATFIEPVWS
                     SLIDGAMYCH"
     gene            complement(416..763)
                     /locus_tag="VIH70_05390"
     CDS             complement(416..763)
                     /locus_tag="VIH70_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8115829.1"
                     /translation="MRATIRCVVVALALVLLAPAVGAHERGGESSGATLVARVRVIDF
                     GFRPRTLMIARGTVVRWVNRGARTHTTTSNTGLWNHRLAPGETFRRRFRRMGTFAYHC
                     SIHPQMTGTITVS"
     gene            885..1694
                     /locus_tag="VIH70_05395"
     CDS             885..1694
                     /locus_tag="VIH70_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115830.1"
                     /translation="MGEAIEIQVDRPEVVEPVESEARTWYGIDGPRRRPRTEVAAPTP
                     SVVIPSDEADVVVGPEDAASRGSPETGHPTVRDRWREESEERARDLAPERGIAPVPRD
                     TETAPGDAHDLEERFEMLRVTMTLELGRRIDAALARMEGTFEREIEALRTANREEAKR
                     LRSTNGEAIARIRADDGRELERIRRAIDEGIERLCGVLEGQLDRVWGANDVELERIRS
                     AGADRLAEVHDLLTDQLERLRMERPQGPPPPEPAPAPRPRRWRRSRGDTSG"
     gene            complement(1701..2747)
                     /locus_tag="VIH70_05400"
     CDS             complement(1701..2747)
                     /locus_tag="VIH70_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8115831.1"
                     /translation="MAGSVAALVLACLPGVALSSSATTDDRLKESKQELHRTKDKVRT
                     QQMRIRKLQRRMNGLATRISKTQSDVVAAEERIDKLERRILVMRVRASVLQAKLDERS
                     RQAYMFGGAPVLYLLTATSAAEAASRMSFLTEMNRRDAVLAAKVRETEDRLARSEGEV
                     LRARQVIELTKRRLELDQKELRRTMAESERLVAGLRDRVEQIQYEISEIRPFSVCPVV
                     GPAPIADDFGIMVHHPPKEGGDHIHQGNDIYAPMGAEIVAPFDGTAETASNRIGGLAV
                     KVYGEFGYVYNAHLSAFGQLGPVEAGDVIGYVGATGNAGGPHDHFEWHPDDGPAVDPH
                     EFLLMVCDSGALPF"
     gene            2951..4435
                     /locus_tag="VIH70_05405"
     CDS             2951..4435
                     /locus_tag="VIH70_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8115832.1"
                     /translation="MGRLPRLRRVRTGSLVVLVVVLFPAATLAMSGLEGDRREADPRP
                     AARSAAAPSPDVHVGPEGIFNLDHLIFIVQENRSFDHYFGTYPGADGLEMVGGVPTNC
                     VPDPVLGRDSCVYRSSADEFNGGPHSRPSALTAINGGAMDGFIDALPPTPRWCVDRAT
                     PECADFLGPEGQPDVMSYLTRRNIPNYWAYADAFVLQDRMFAPTDGWTLPAHLFLVSG
                     WSAYCYHPGDPMSCVSDVGLKEEHQRFEYGEAPIYAWTDITWLLDREGVSWGYYVAPG
                     TCSFPPCHGRAIDVREGTTPASKNPLPGFATLYETRQRDHIRDYRSFERRARRGVLPS
                     VSWVVPGDGVSEHPQSSRGVAAGMAHVTRIVNSVMRGPDWDSSAIFITWDDWGGFYDH
                     VVPPLADENGYGLRVPGLLVSPYARRGFVDHQTLTFDAYLKLIEDRFLGGQRLDPSTD
                     GRPDSRPVVREALPVLGDLALAFDFDRRPRAPLILDPTPQTRPR"
     gene            4549..6168
                     /locus_tag="VIH70_05410"
     CDS             4549..6168
                     /locus_tag="VIH70_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8115833.1"
                     /translation="MVIRVPDQGNTLGGYAALDDRLLVLDFQAGNPQAFVEIHRRYGP
                     LARRVCQRFLPNPHDGDEAFQETMIRVYQGLYRFNGRYAVQPWVARIAKNVSLDILRG
                     RARRPKNDDWAPLAEELPAPNDEAEQIVERLVQHDMVLTVLSDLPETHRRALMMRELD
                     GRSHREIAQEMEVSTAQAKALIHRAKMSFRKRWLEKVAERGGLMGIGLVPLVWLLRIA
                     GGLRRIGERFGHSAQLAQAAVPEAVSSTVGSAAAPVVSSGMGERVVAAGMTLLLAGGV
                     TVGAAKIVKGGDQDEPRARAVAAAPAPTLDASRVGATAPRVDRPVSDSGRTRDAGDLR
                     AEPPIVDKPDPSQVADPSPVVEPSPSVDPSPSPDADPSPEPTVTPPPEPAPEWTMSFG
                     SDLLGDTSWLSLVNSKIQGKMGNTVRYSQTVTGSLVGSKRALTRINLEYWGSVQGTAG
                     NVELRLFLDSPAGRYEYRASASVISVNIAPDGSAIYRFRGSYMLTKAPVAAEAIMPHD
                     GSITLTLGFWSNETSDEISLFANALELVEAS"
     gene            6563..7900
                     /locus_tag="VIH70_05415"
     CDS             6563..7900
                     /locus_tag="VIH70_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY8115834.1"
                     /translation="MTIDSGNSAVIGVDTTPTPDEIIFDPDQTLAGAAQCSTADVTNT
                     NLIDVDGSTGSETFVVDLSGGAFASNNDFNIDLGTGTDTLRIIGSDGADTISFASATS
                     ISLGGGTDITLASGSVETFSVNAGTGNDNVSGGTFATAITINGEAGDDTLTGGSGVDT
                     INGGAGNDTISGGTGTANDTLTGGDGTDTLTYAAIAAAVTVNLATTTAQTTGGAGSDT
                     ISTFENLTGGLGADNLTGSTGVNTILGGAGNDTVNGGDGNDNLQGDAGDDTLIGGVGD
                     DNINGGDGTDTVSYAGTTAAVTVNLSVTTAQATGGAGSDIITNAENLTGGSGGDTLTG
                     TSGTNVVAGEDGNDILDGGDGNDTLRGGAGDDSAKGSAGDDSLRLGSGNDAGRGGGGN
                     DALRGGGGNDTLRGAGGNDNIRGGAGNDRIRGGPGNDTCKGGPGNDNIKGCEH"
     gene            8104..9144
                     /locus_tag="VIH70_05420"
     CDS             8104..9144
                     /locus_tag="VIH70_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115835.1"
                     /translation="MAWIPIGVAAAALMRGLSRRPRVAAVLGVLLASFVVGLSAVPAY
                     AATCSGGATMTIDLAGGESIALSLSGGADPLGIVVTPSDPSCGGFDTSTVTAIHVNGT
                     GGDESVTIDQAGSAPFPHQNTVSIDLALGDGTDSIVITGQATADAIGFGANGISLDAG
                     GTPDVTGIGTVESFTVDAGAGDDTVSGKEGGGLGGNFATALTITGEDGDDVLSGGDGN
                     DAIDGGSGNDTIRGGTGDDIVDGASGADVVSGGDGNDSVEGGGGKDGLKGGDGSDTLD
                     GGAGADTLSGGEGADTVNGGEGNDRLKGGGNDDDVNGDPGRDELAGGHGVDRCLGGPD
                     PDSITGCESGHP"
     gene            complement(9178..>9436)
                     /locus_tag="VIH70_05425"
     CDS             complement(9178..>9436)
                     /locus_tag="VIH70_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEY8115836.1"
                     /translation="IGVTATGRLLLVVIDGRRPRWSRGATLGELRGILRDLGAVDALN
                     LDGGGSSEMVVQGEVVNRPSDGHERRITNAVLVLPGADPGE"
BASE COUNT         1526 a         3203 c         3306 g         1401 t
ORIGIN      
        1 tcgtggcagt acatcgcccc gtcgatcagg gaggaccaga cgggttcgat gaacgtcgcg
       61 agctgctcga tcgtcggctt gcggaactcc ggcgccgtct cggcgacgaa gtcgatccag
      121 aactggcccg cgtcctggtc gccctcggcc ggcggcgaga ggtcgacgag atcctcctcg
      181 aaacccagcg gctcgagctt ccccttccac gacgcctcgg ccgcgaacac gacgcggtgc
      241 ccccgccgtt cgagcacctt gccgatgccg atgcagttgt tcgtcggccc gtaggcgctc
      301 tcgggcatga acacgatcgt gcgccgttcg cccgccatct gcgccgcacc ctaccccagc
      361 ggctccggga cgcgcccgac cgcgacaccg atcgagtgtg ggcccacgca cgtccctacg
      421 agacggtgat cgtccccgtc atctgcgggt ggatcgagca gtggtaggcg aacgtcccca
      481 tccggcggaa gcgtcgtcgg aaggtctcgc cgggcgccag tctgtggttc cacaggcccg
      541 tgttcgacgt ggtggtgtgg gtccgggcgc cgcggttcac ccaccggacg acggtgcctc
      601 gagcgatcat cagggtccgc ggacggaacc cgaagtcgat gacccgcacc cgggcgacca
      661 gcgtcgctcc gctggattcg cctccccgct cgtgcgcgcc gactgccggt gcgagcagga
      721 cgagggcgag cgcgacgacg acgcatcgga tcgtggcacg catccgtggg ctccttctcg
      781 ggtcgggttg agcgggcctg ggccggccat cgtagtgcag gtcgttgaat cgggcgaccg
      841 gctgtcctac ggtcggagcc atcggaacgt caccgggcgc ggaggtgggg gaggcgatcg
      901 agatccaggt ggaccgcccc gaggtcgtcg agcccgtcga gagcgaggcg cgcacgtggt
      961 acgggatcga cgggccgcgt cgccggccgc gaaccgaggt cgcggctcca acaccctccg
     1021 tcgtcatccc ctccgatgag gccgacgtcg tcgtcggccc ggaggacgcc gcctcccggg
     1081 ggagccccga gaccggacac ccgaccgttc gggaccgctg gcgcgaggag tcggaggagc
     1141 gtgcccgcga cctcgcgccc gaacgcggga tcgcgccggt gccccgcgac accgagaccg
     1201 cgcccgggga tgcccacgac ctcgaggagc ggttcgagat gctgcgcgtc acgatgacgc
     1261 tggagctcgg gaggcggatc gacgccgccc tcgcgcggat ggaggggacg ttcgaacggg
     1321 agatcgaggc gctccggacc gcgaaccgcg aggaggccaa gcgcctccgc agcacgaacg
     1381 gcgaggccat cgcccggatc cgggcggacg atggccggga gctcgaacgc atccggcgcg
     1441 cgatcgacga gggcatcgaa cgcctgtgcg gcgtcctcga gggtcagctc gaccgggtct
     1501 ggggcgcgaa cgacgtcgag ctcgagcgca tccgttccgc cggcgccgac cggctggcgg
     1561 aggtgcacga cctcctcacc gaccagctgg agcgactgcg gatggagcga ccgcaggggc
     1621 cgcccccgcc ggagccggcc ccggcgccca ggcctcgccg gtggcgccga tcccggggcg
     1681 atacgtccgg ctgaccgcgc tcagaaggga agggcgccgg agtcgcagac catcagcagg
     1741 aactcgtgcg ggtcgaccgc ggggccgtcg tccggatgcc actcgaaatg gtcgtgtggg
     1801 ccgcccgcgt tacccgtcgc gcccacgtag ccgatcacgt cgcccgcctc caccggtccg
     1861 agctgcccga acgcggacag gtgcgcgttg tacacgtagc cgaactcgcc gtacaccttg
     1921 accgcgaggc ccccgatgcg gttcgaggcc gtctcggccg tgccgtcgaa cggcgcgacg
     1981 atctccgcac ccataggcgc gtagatgtcg ttgccctgat ggatgtggtc gccgccctcc
     2041 ttcgggggat ggtggaccat gatgccgaag tcgtccgcga tcggtgccgg accgaccaca
     2101 gggcacaccg agaagggccg tatctccgag atctcgtact ggatctgctc gacgcggtcg
     2161 cgcagcccgg cgacgagccg ctcggactcg gccatcgtcc ggcgcagctc cttctggtcg
     2221 agctcgaggc ggcgcttcgt gagctcgatc acctgacggg cacggagcac ctcgccctcg
     2281 ctgcgcgcca gccggtcctc ggtctcgcgc accttggccg ccaggacggc gtcccgacgg
     2341 ttcatctcgg tgaggaagga catccgcgac gccgcctcgg ccgccgacgt tgccgtcaac
     2401 aggtacagga ccggcgcgcc gccgaacatg tacgcctggc ggctccgctc gtcgagcttg
     2461 gcctgcagga cggaggcgcg tacccgcatg accaggatcc gccgctcgag cttgtcgatg
     2521 cgctcctccg cggccacgac gtccgactgg gtcttggaga tcctggtcgc gaggccgttc
     2581 atccgccgtt gcagcttccg gatcctcatc tgctgggtcc ggaccttgtc cttggtgcgg
     2641 tgcaactcct gcttggactc cttgagccgg tcgtcggtcg ttgcggagct cgacagggcg
     2701 acccccggca ggcaggcgag aacgagcgcg gcgacggatc ctgccacgat gcgatgggcg
     2761 cgcggaacgg cgcctcgagt ggtgcgcggg ccggccatct cggtcgctcg aacgtacccg
     2821 cgcatacgca cggcacaacc gcgctcggtc gtaggccgcg gggagcacga gacccctttc
     2881 ggggcggctc gaggcccatc cgtcccacat gcttgtctcg ggcaggccgc gggctagact
     2941 ccacctcccc atggggcgcc ttccccgcct tcgccgtgtc cgcacgggtt cgctcgtcgt
     3001 actcgtcgtg gtgttgttcc ccgcggccac cctggcgatg tccggcctag agggggaccg
     3061 tcgggaggcg gatccgcggc ccgccgcccg gtcggcggcc gccccctcac ccgatgtcca
     3121 cgtggggccg gaggggatct tcaatctcga ccacctgatc ttcatcgtgc aggagaaccg
     3181 ctcgttcgat cactacttcg ggacctaccc gggcgccgac ggtctcgaga tggtcggcgg
     3241 cgtgccgacg aactgcgtcc ccgatccggt cctgggtcgg gactcgtgcg tgtaccgctc
     3301 gtcggcggac gagttcaacg gtggtccgca cagccggccc tcggcgctca ccgcgatcaa
     3361 cggcggcgcc atggacgggt tcatcgacgc gctcccaccc accccgaggt ggtgcgtgga
     3421 ccgcgcgacc cccgagtgcg ccgacttcct gggtcccgaa gggcagcccg acgtgatgag
     3481 ctacctcacc cggcggaaca tcccgaacta ctgggcgtac gcggacgcgt tcgtgctgca
     3541 ggaccggatg ttcgccccca ccgacggctg gacgctgccc gcgcacctgt tcctcgtctc
     3601 gggctggtcc gcctactgct accacccggg cgacccgatg tcgtgcgtct ccgacgtcgg
     3661 cctgaaggag gagcaccagc ggttcgagta cggcgaggct ccgatctacg cgtggaccga
     3721 catcacgtgg ctgctcgacc gcgagggtgt gtcctggggc tactacgtgg ctcccggcac
     3781 ttgctcgttc ccgccgtgcc acgggcgggc gatcgacgtt cgggagggga cgaccccggc
     3841 gtcgaagaac ccgctcccgg ggttcgcgac cctgtacgag acgcggcagc gcgatcacat
     3901 ccgggactac cggagcttcg agcgaagagc ccggcgcggg gtgctcccgt ccgtgtcgtg
     3961 ggtagtgccg ggcgacggcg tgagcgagca cccccagtcg agccgcggcg tcgccgccgg
     4021 catggcccac gtcacccgga tcgtgaactc ggtgatgcgc gggccggact gggacagctc
     4081 ggcgatcttc atcacctggg acgactgggg aggcttctac gaccacgtgg tgccgccgct
     4141 cgccgacgag aacggatacg ggcttcgggt gcccgggctc ctcgtgagcc cgtacgcccg
     4201 gcgcgggttc gtcgatcacc agacgctcac gttcgacgcg tacctgaagc tgatcgagga
     4261 tcggttcctg ggcggtcagc gcctggatcc gtccaccgac gggcgtcccg actcgcgccc
     4321 ggtcgtgcgc gaggccctgc cggtcctggg ggacctcgcg ctggcgttcg acttcgaccg
     4381 gaggccgagg gcgccgctga tcctggaccc cactccgcag acccgtcctc ggtgagcgag
     4441 ggcctctcga ggggagcacg ccgcgtgcta cagtcctcca tccgggccgg gatgtatggc
     4501 tcaagcaacc ctggggcgct gccgacgttg ttcctttagg agataactgt ggtcatcagg
     4561 gttcccgacc agggcaacac gctgggcggc tacgccgccc tggatgaccg tctcctcgtc
     4621 ctggatttcc aggccgggaa cccacaggcg ttcgtcgaga tccatcgccg ctacgggccg
     4681 ctcgcccgcc gggtgtgtca gcggttcctc ccgaatcccc acgacggcga cgaggccttc
     4741 caggagacga tgatccgggt ctatcagggc ctgtacaggt tcaacggccg ctatgccgtg
     4801 cagccctggg tcgcccggat cgccaagaac gtctccctcg acatccttcg cggtcgagcc
     4861 agacgcccca agaacgacga ctgggcgccc ctcgcggagg agcttccagc tccgaacgac
     4921 gaggcggagc agatcgtgga gcggctggtt cagcacgaca tggtgctcac cgtcctctcc
     4981 gacctgcccg agacacaccg ccgcgcgctg atgatgcgcg agctggatgg acggtcgcat
     5041 cgcgagatcg cgcaggagat ggaggtctcc acggcgcagg ccaaggcgct gatccatcgg
     5101 gcgaagatga gtttccgcaa gcgatggctc gagaaggtgg ccgaacgagg cggcctcatg
     5161 gggatcggtc tggtgccgct cgtctggctc cttcggatcg cgggcggctt gcgcaggatc
     5221 ggcgagcgat tcggtcactc ggcgcagttg gcacaggccg ccgtaccgga ggcggtcagc
     5281 tcgacggtgg gatcggcggc ggcgccggtc gtgtcgagcg ggatgggcga gcgcgtggtc
     5341 gcggcgggga tgacgttgct cctggcgggc ggggtcaccg taggagcggc caagatcgtg
     5401 aagggtggag accaggacga gccacgggcg cgagcggtcg ccgcagcgcc ggcaccaact
     5461 ctggatgcct ccagggtcgg cgcgacggct ccgcgcgtcg accgtccggt ctccgattcc
     5521 gggcgaaccc gtgacgccgg tgacctccga gcggagccgc cgatcgtcga caagccggac
     5581 ccgagccagg tggcggatcc gagccccgtg gtcgagccga gcccgagtgt cgacccgtct
     5641 ccatcaccgg acgcggatcc gtcgccggaa cccacggtga cgccgccgcc cgagcccgcg
     5701 cccgagtgga cgatgagctt cgggtccgat cttctcggtg atacatcgtg gctctcgctc
     5761 gtgaactcga agatccaggg caagatgggc aataccgtcc gctactcaca aaccgtgacg
     5821 ggatctctgg ttgggtcgaa gcgtgcgctc acccgtatca atctcgagta ttgggggtcg
     5881 gtccaaggca cggcaggcaa cgtggaattg aggctgttcc tggactcgcc ggccggaagg
     5941 tacgagtacc gggcgtcggc gtcggtcatc tcggtcaaca tcgctccaga cgggagcgcc
     6001 atctacaggt tcaggggctc gtacatgctc accaaggctc cggtcgccgc cgaagccatc
     6061 atgccgcacg acgggtcgat cacgctgacc cttggcttct ggagcaacga gacctccgac
     6121 gagatctcct tgtttgcgaa cgccctggag ctcgtcgaag catcctgatc gaccttctcg
     6181 gcgaccgcaa caaccttccg ggccgcaggc cgttctaact aggcggagat cgaagctgtg
     6241 catgtggtgc cgccagcgtt cgatgcactt cggactcgga aaggcgtcca catcatggtc
     6301 ggtcagtcca atcgatccgt tcgagtttgg gttcgacgga tcccgcgcat ggggtccgga
     6361 tgcaccggag gaggcccggg acgctcggtg aaaggggaag gggatgctga accaacagag
     6421 gtgggaaggc gcagccgctg gacggggaca gcggctggct tcgttcctcg ttgtctcgtt
     6481 tacggtaatg ctaatcatgc tgttcttggc acctgcagct agcgcagttc cgacatgcac
     6541 gttcgcgggc agtaccgcga cggtgacgat tgactcgggg aactccgcgg tcatcggcgt
     6601 tgacaccacg cccacaccag acgagatcat cttcgaccca gaccagacgc ttgctggcgc
     6661 cgcgcaatgc agtacggcgg acgtgacgaa cacgaacctg atcgacgtgg atgggagtac
     6721 cggcagcgaa accttcgttg ttgacttgtc tggcggtgcc ttcgcgtcga acaacgactt
     6781 caacatcgat ctgggaacgg gcaccgacac gctcagaatt atcggatccg acggcgctga
     6841 cacgatcagc ttcgcatccg caacgagcat ttcgctcggc ggtggcaccg acatcacgct
     6901 cgcgtcgggt tcggttgaga cattctccgt gaacgccggg accggtaacg acaacgtgag
     6961 cggtgggacg ttcgcgacgg ccatcaccat caacggcgaa gcgggagacg acaccctcac
     7021 cggcgggagc ggcgtcgaca ccatcaacgg aggcgccggg aacgacacca tcagcggcgg
     7081 tacgggcacg gcgaacgaca cattgaccgg tggcgacggc acggacacgc tgacctacgc
     7141 ggctatagcg gccgccgtga ccgtcaacct cgccaccacg acggcacaga ccaccggtgg
     7201 ggccgggtcc gacacgatct cgaccttcga gaacctcacc ggtggattgg gtgccgacaa
     7261 tctgacgggg agcaccgggg tcaacaccat cctcggtgga gccgggaacg acaccgtcaa
     7321 tggtggcgat ggcaacgaca acctgcaggg tgatgccggg gacgacactc tgatcggtgg
     7381 tgtcggggac gacaacatca acggtggtga cggcaccgat accgtgagct acgcaggcac
     7441 tacggctgcg gtcaccgtga atctatccgt gacgaccgcc caggccaccg gtggagcagg
     7501 ctcggacatc atcacgaacg ccgagaacct gaccggcggt agtggcggcg acacgctcac
     7561 cggaaccagt ggaaccaacg tggtcgccgg tgaagatggc aacgacatcc tcgacggcgg
     7621 tgacggcaac gatacgttgc gcggcggggc cggtgacgac agcgccaagg gtagcgcagg
     7681 ggatgactcc ctcaggctcg gcagcggcaa cgatgcaggt cgcggcggcg gcggcaacga
     7741 cgcgctccga ggcggcggcg gcaacgacac gctccgaggc gccggcggca acgacaacat
     7801 ccgcggcgga gccggcaacg accgaatccg aggagggccc ggcaacgaca cctgcaaggg
     7861 cggccccggc aacgacaaca tcaagggctg cgagcactga cgtagcatcc ggcggacaac
     7921 ggccctcggc gctctgccgg aggccgttcc gtcgcgccgg gaaggcctca cgtggggcgg
     7981 gcgaaactcc gagagggcat cggggtcggc tcccctttgg cctcccggcg agcctgaatg
     8041 ccacgacctg aatcccacga aacgcatctt ggcgggtgct agtgtcggcc gcggcatggg
     8101 gcgatggcct ggatcccgat cggcgtggcg gccgcggcgt tgatgcgtgg cctctcccgc
     8161 cgaccccggg tcgcggcggt gttgggcgtg ctcctcgcgt cgttcgtcgt cggcctgagc
     8221 gccgtcccgg cttacgcggc aacgtgttcg ggcggtgcca cgatgacgat cgatctcgcg
     8281 ggtggggagt ccatcgcact ctcgttgagc ggtggggcgg atcccctcgg catcgtcgtc
     8341 acgccatccg atcccagctg cgggggcttc gacacatcga cggtgaccgc gatccacgtg
     8401 aacggcaccg gtggggacga gagcgtcacc atcgaccagg ccgggagcgc accgttcccg
     8461 caccagaaca cggtctcgat cgatctcgca ctgggcgacg gcacggattc catcgtcatc
     8521 accggacagg ccaccgccga cgcgatcggg ttcggcgcca acggcatcag cctcgatgcg
     8581 gggggaaccc ccgacgtcac cgggatcggc acggtggaaa gcttcaccgt cgatgccggc
     8641 gccggagacg acaccgtctc gggcaaggaa ggcggcgggc tgggaggcaa cttcgcgacg
     8701 gcgctcacga tcaccggtga agacggcgac gacgtcctct cgggtgggga cgggaacgac
     8761 gccatcgatg ggggaagtgg gaacgacacg atcaggggcg gcaccggtga cgacatcgtc
     8821 gatggcgctt ccggtgcaga tgtcgtctcg ggaggcgacg ggaacgactc cgtcgaaggc
     8881 ggcgggggga aggacggact caagggcggt gatggcagcg ataccctcga cggcggcgcc
     8941 ggggcggaca cgctgagcgg tggggaaggc gccgacaccg tcaacggtgg tgaggggaac
     9001 gacaggctca agggcggcgg caacgacgac gacgtgaacg gcgaccccgg aagggatgag
     9061 ctcgccggtg gtcacggcgt cgaccgttgc ctcggggggc cagaccccga ctcgatcacc
     9121 ggttgcgaga gcggacaccc gtaacctcgg ggcgaacccc gcgcgggccg cgcccggcta
     9181 ctcgcccggg tcggcgccgg gaaggaccag gaccgcgttg gtgatcctcc gctcgtgtcc
     9241 gtccgaaggc cggttcacga cctctccctg gaccaccatt tcggacgagc ctccaccgtc
     9301 caggttcagc gcgtcgacgg cgcccaggtc cctcaggatg cctctgagct cgcccagcgt
     9361 cgcgccccgt gaccaccggg gccggcggcc gtcgatcacg accaggagga gcctgccggt
     9421 cgcggtgaca ccgatc
//
LOCUS       DATBRK010000180        10720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1618299, whole genome shotgun sequence.
ACCESSION   DATBRK010000180 DATBRK010000000
VERSION     DATBRK010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10720
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618299"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VIH70_05430"
     CDS             <1..1245
                     /locus_tag="VIH70_05430"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HEY8115837.1"
                     /translation="EKATGEPVRRIMDGWIWQGGYPLVSARLRNGTVELGQRRYLISG
                     EEDRTVWDVPLLVRTSGGTEPVLIEPGGVSIPVPGDGQVVVNAGVHAFVRVEYDDELL
                     ARLTADLRSLSTDERTQLVDDTWAAVIAGRRSAVDFCRFAASFADETELPVWQSLLQG
                     LAWCERFLTDGPREHFRSWIRGLLWPAIQRIGWEPREGERDLDKPLRGALMAALGVLG
                     ADPQAFGLAMEIERESRGDGQVDPSLAAASVAIVAAGGTAEDYGRYLEAIGSPRTPQE
                     ELRYLYALPDFRDPELIRRTVEKTLTGEIRSQNAPGVLARAVANRDHGDLAWSFVKEH
                     WDEIVSRLAPTTLVYAADGVRYLTEPGQVEDAAAFFAAHPIPQSTLQLRQVLERQRVN
                     QAFRTRATPELMGAFSAGPELS"
     gene            complement(1242..2186)
                     /locus_tag="VIH70_05435"
     CDS             complement(1242..2186)
                     /locus_tag="VIH70_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY8115838.1"
                     /translation="MDRPTIGRRDIEAADDRIRPYVRRTPVLELGRGAFDLACRVTLK
                     LELMQHTGSFKPRGAFNKILSSDVPESGVIAASGGNFGLAVAFAALTLGHRAEIFVPS
                     NSPRVKADRIRTMGAEVTVVEGWYDEALEASHGRAAETGALVMHAYDQPEVVAGQGTL
                     GVELADQVPDADTVLVAIGGGGLIGGVAGWYEGRTRVIGVEPETSRSMTAALAAREPV
                     DVEYGGYAADSLGTRRAGEIAFAIVSKHVERVVLVPGDAIRDAQRRLWREVRVFAEPG
                     GAAALAALLAEAYEPEPDEHVVALVCGANGELGVLSED"
     gene            2377..3837
                     /locus_tag="VIH70_05440"
     CDS             2377..3837
                     /locus_tag="VIH70_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY8115839.1"
                     /translation="MEGVRISTEHWIGGERVGSSETFDDVSPIDEQVIARVARGGPAE
                     ADRAVEAARAAFDTWGRTSPEDRAKVLHRVAELVEERVPELAAVETGDNGSLLRSHLN
                     SVIPRVARNFRFFADQLTELGDEAFEMNEFVSKTEWDPSGVTIVITPWNAPLMLATWR
                     IAPALAAGNSVVAKPPEWAPLTASLLADITREAGLPDGAFNVVQGIGEEAGAALTSHP
                     KVDRIAFTGSIETGKLVAQAAAANLTPVSLELGGKSPFVAFADADLDATVKQAVNQFD
                     NAGQVCLAGTRLIVERSMRDAFLERFLEAAGAIRQGDPREETTDIGPQITREHFERVD
                     GFVQRAKADGAEVLLGGGPNEELGGLFYRPTLFGDVPKGAEVLRKEVFGPVLTLQTFE
                     DEEEAIALANETDYGLAAILYTGDRERAGRVSERLVAGTVWVNCFFVRDLRAPFGGAK
                     SSGVGREGGVYSFDFYADVKNVCSAPWPQGAEREAI"
     gene            3854..4738
                     /locus_tag="VIH70_05445"
     CDS             3854..4738
                     /locus_tag="VIH70_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HEY8115840.1"
                     /translation="MGEIVGAALVAHVPTIMLPEEVRLEINEGKEITLVPGLHRIKAE
                     CLDRLQPDAVIVFDTHWESTFEHIVTSHDRRQGKFTSHELPRGMKQIPYDMPGDPELA
                     NLIAAQAEGRDDCWILANDDPYLPLFYGTVNIWTFLGDPNTAWLSVALNQCCDTNDFL
                     LFGELIGKAIEQSDRRVVLLASGGMTHRFFNFKDLRKRESQKAPDNIYDRAYYDADMG
                     VLQKFEKGDHAGVIDGMPEYRKAYPEGHFGHYLMMAAALGGRDCNATGELFSEYEASV
                     GTGQVHVWFERPEGGWTA"
     gene            4791..6266
                     /locus_tag="VIH70_05450"
     CDS             4791..6266
                     /locus_tag="VIH70_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8115841.1"
                     /translation="MEGERPLVVTEEYERLGGKKLSLIDVLAQSVGFMGPVFSAAFII
                     PLIIGVNASGRGAGTSAALAVLLAAIGVFALGWIVAQYAKRIHAAGSLYDYVSNGLGK
                     TFGAASGWLYYGGTIILTTGLGLLIGGYVHDNLLPTLEIDLDLPIWLWDTIFALGLFA
                     VLYFGVQISTRVQLTLALISIAVVLIFFITVIADLGGDNDIAKAFDPSPAGGFSGILF
                     GVLYGVLIFVGFETAANLAEETAEPKRSIPRAVLGAVAIVSIFYLIAAYVEVAGFGFD
                     LAVITSPEVAGAPLFALGAPPGPPGSEFWLKILLVVVFLDMLAVYVGAAVASTRGVFA
                     MARDRRLPGGLAAVSKRYGTPIGAILLLIAIQAVLIVVSEANDTLLALPGLPHYFSVF
                     VWCATFGGFALLVVYFLMSIGAFRGVGGDTATVGIIVAALVGIAITAAAIFGSFYKVT
                     SPTLLAPWSAVIWFAIGLVYMALVKGREPASDALADLRTSA"
     gene            6266..7240
                     /locus_tag="VIH70_05455"
     CDS             6266..7240
                     /locus_tag="VIH70_05455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY8115842.1"
                     /translation="MPEGGGGAIRPRLVLILSENWTLVPARDLGALVRIAVDAEEAGF
                     DAAMVSEHVVLGRGADANGVPQNPRDYALPGNQDPSTPWPDALTLLSSVASATTTLRL
                     VASSIIVPLRHPLLLAKQMATLDLLSRGRLVVQPTVSWHRPEYEALGVPFERRGELLD
                     DHLAAWRVLWRDTPASFEGTHYRFSDVTLEPKPHRPGGPVLWFGGETVHPRLLRRLVE
                     YGSGFNPLGRPSNEDLERLLSAMAEAGREMSEIEMVGGTRGRFEDAESVADLGEALSS
                     IPEQLERGFTTICIKPSQFIDDPDGIGTFCREVVERVDALPPPQMTSK"
     gene            complement(7222..8568)
                     /locus_tag="VIH70_05460"
     CDS             complement(7222..8568)
                     /locus_tag="VIH70_05460"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY8115843.1"
                     /translation="MTLDLSDLDDFATVVVTSADVTGRPVGKRVSVDVFRRVVQEGIP
                     LSSCVLGWDVDQWPGPVQEYTGHHTGWHDVRLVPDLGTLRPTAWLERTAICLADFVEM
                     RSDELAPVAPRTILRRQIERLVATGLTAQVASELEFALYHGSYDEGRANGYETLPPTT
                     LARADYTIQAGDRYERFFSGVRDALAASELGPWTSQVEWGLGQWEINLEYQHALEMAD
                     RHLLFKLAMRTLAADAGMAVTFMARPFADTTGSSCHLHLSLVDGDGRNVFHDEEDAGG
                     VSSTLRHAIGGVLQRGPELMLFYAPTVNSYRRTTSGEFSGNGLSWGFDSRMVSCRVLT
                     ESPASTRLEWRVPGADVDPYLAIAALLASARDGIETDAEPGEPLMGSDYERPVAPLPA
                     TLGEAVERFRDGGFSAEAFGKDVVAHYAEAGRWEWEQFLAPGAVGEWERRRYFDVI"
     gene            complement(8565..9335)
                     /locus_tag="VIH70_05465"
     CDS             complement(8565..9335)
                     /locus_tag="VIH70_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8115844.1"
                     /translation="MSFDHSGRVALITGAGSGIGRATALRLASEGAAVAVVDVRLDAA
                     EETVAAIADGGGTGVALEADVRDRSQIESARDGAVNAFGHIDYLVNNAGLVRMSPLDE
                     LAEEEWDLVLDTNLKGMYLVTQAIAPVIAGEGGGAIVNLATIESEVVVASTGHPQVHY
                     NASKGGVKMLTKALAVELADRNIRVNAVEPGAIATSFIPGGNIRSPEAWASMKDRLLV
                     PRVGEPEDVAAAISFLLSDDASYITGAMLPVDGGWLAR"
     gene            complement(9919..10716)
                     /locus_tag="VIH70_05470"
     CDS             complement(9919..10716)
                     /locus_tag="VIH70_05470"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8115845.1"
                     /translation="MRLEGKVAIVSGGGTGIGAAAARLFAREGAKVVVTGRRAEPLEE
                     VAAETGCRVVAGDTADEDHVREAVAAAVEAFGGLDIVVANAGIGFGGAALDVDDERWD
                     RTLDVNVTGAFRLARAAIPTLIERGGGSIVLVSSVNAFVSGTESAAYGTSKAAMNGLA
                     RSIAVDYGPRGVRANALCPGWVVTPMGDRAMQDLMTERGISLEEAYDLVTRHTPLRRP
                     ATADEIAACCLFLASDESSIVTGTALVADGGGLAMDLTEVAYQQERP"
BASE COUNT         1563 a         3743 c         3784 g         1630 t
ORIGIN      
        1 gagaaggcca ccggcgagcc ggtccgcagg atcatggacg gctggatctg gcagggcggg
       61 tacccgctgg tctcggcgcg gctccgcaac ggcacggtcg agctcggcca gcggcggtac
      121 ctgatctcgg gcgaggagga ccgcaccgtg tgggacgtgc cgctgctcgt tcggacgtcc
      181 ggggggaccg aacccgtgct gatcgagccg ggcggcgtgt cgatccccgt cccgggtgat
      241 ggtcaggtgg tcgtgaacgc cggcgtacac gcgttcgtcc gagtcgagta cgacgacgag
      301 ctgctcgcgc gcctcacggc ggacctgcgt tcgctctcga ccgacgagcg cacacagctc
      361 gtggacgaca cgtgggcggc ggtgatcgcc ggtcgacgtt cggccgtcga cttctgccgg
      421 ttcgcggcgt cgttcgcaga cgagaccgag ctcccggtgt ggcagtcgct gctgcaaggc
      481 ctcgcctggt gcgagcggtt cctgacggac ggcccgcgcg agcacttccg ttcgtggatc
      541 cgaggactgc tgtggccggc catccagcgg atcggatggg agcctcgcga gggggagcgc
      601 gacctggaca agccactccg cggcgccctg atggcggcgc tcggtgtcct cggcgccgac
      661 ccccaggcgt tcggcctggc gatggagatc gagcgcgagt cgcgcggcga cgggcaggtc
      721 gatccgtcgc tcgcggcggc ctcggtcgcg atcgtcgcgg ccggcggcac ggccgaggac
      781 tacgggcggt acctcgaggc gatcggatcg ccgcggaccc ctcaggagga gctccgctac
      841 ctgtatgcgc tcccggactt ccgggacccg gagctcatcc ggcggacggt ggagaagacg
      901 ctcaccggcg agatccgctc gcagaacgcg cccggcgtgc tcgcgcgcgc ggttgccaac
      961 cgggatcacg gggacctggc gtggtctttc gtgaaggagc attgggacga gatcgtgtcg
     1021 cgcctcgcgc cgaccacgct cgtctacgcg gccgacggcg ttcggtacct caccgagccc
     1081 gggcaggtcg aggacgccgc cgcgttcttc gccgcgcacc cgatcccgca gtcgacgctc
     1141 cagctccggc aggtcctcga gcgccagcgc gtgaaccagg cgttccgcac gcgagctacg
     1201 cccgagctca tgggcgcgtt ctccgccggc cccgagctct cctagtcttc cgacagcacc
     1261 ccgagctcac cgttcgctcc gcagacgagg gcgaccacgt gctcgtccgg ctccggctcg
     1321 tacgcctctg cgagcagggc cgcgagcgcc gccgcgcctc cgggttcggc gaacacgcgg
     1381 acctcgcgcc agagccgtcg ctgcgcgtcc ctgatcgcgt cgccgggcac gaggaccacc
     1441 cgctcgacgt gcttggagac gatcgcgaac gcgatctccc cggcccgccg ggtcccgagc
     1501 gagtcggccg cgtacccgcc gtactcgacg tcgacgggct ctctcgccgc cagcgcggcg
     1561 gtcatggatc ggctggtctc gggctcgacg ccgatcacgc gggtccggcc ctcgtaccag
     1621 ccggcgaccc cgccgatcag gccgccgccc ccgatcgcga ccagcacggt gtcggcgtcg
     1681 gggacctggt cggcgagctc gacgccgagc gtgccctgcc ccgcgacgac ctcgggctgg
     1741 tcgtaggcgt gcatcacgag ggcgcccgtc tcggcggcgc gcccgtggct cgcctcgagc
     1801 gcctcgtcgt accagccctc cacgaccgtg acttccgcgc ccatcgtgcg gatccggtcg
     1861 gccttgaccc tcggagagtt cgaggggacg aagatctcgg cgcggtggcc gagcgtgagc
     1921 gcggcgaacg ccaccgccag tccgaagttc ccgcccgagg ccgcgatcac gccggactcg
     1981 gggacgtcgg acgagaggat cttgttgaag gcgcctcggg gcttgaacga gcccgtgtgc
     2041 tgcatgagct cgagcttcag cgtgacccgg caggcgagat cgaacgcccc tcgtccgagc
     2101 tcgagcaccg gcgtgcggcg cacgtaggga cggatgcggt cgtccgccgc ctcgatgtcc
     2161 ctacgcccga tggtcggccg atccatcctc ggcatgaaac cacgtcatga tccgccaccc
     2221 gtgggagccc gctgttacgt gagccgctcg tccaccagcg cttcaccccg tggtaccgac
     2281 cgccgtcgcc ttgacagttc gccgaacgcg ttcctatcgt tcggaaccga acgacctttc
     2341 ccgaggagag gagcccccac ggccgggacc gccgagatcg agggcgtgcg tatctcgacc
     2401 gagcactgga tcggcggcga gcgggtgggc tcgtcggaga ccttcgacga cgtctcgccg
     2461 atcgacgagc aggtcatcgc tcgggtcgcg cgcggcgggc cggccgaggc cgaccgggcg
     2521 gtcgaggccg cgcgggccgc gttcgatacc tggggccgga cctcgcccga ggaccgcgcg
     2581 aaggtcctgc accgggtcgc cgagctcgtc gaggagcgcg tgcccgagct cgcggccgtc
     2641 gagaccgggg acaacggctc gctcctgcgc tcacacctga actcggtcat accacgtgtc
     2701 gcgcggaact tccgtttctt cgccgaccag ctcaccgagc tcggcgacga ggccttcgag
     2761 atgaacgagt tcgtgagcaa gaccgagtgg gatccttccg gtgtcacgat cgtcatcacg
     2821 ccgtggaacg cgccgctgat gctcgcgacg tggcggatcg ctcccgcgct cgcggccggc
     2881 aacagcgtcg tcgccaagcc gcccgagtgg gcgccgctca ccgcgtccct gttggccgac
     2941 atcacgcgcg aggccggcct gcccgacggc gcgttcaacg tggtccaggg gatcggggag
     3001 gaggccggcg ccgcgctcac ctcgcacccc aaggtcgacc ggatcgcgtt caccgggtct
     3061 atcgagaccg gcaagctggt cgcgcaggcg gccgcggcga acctcacgcc ggtctcgctc
     3121 gagctcgggg ggaagtcccc gttcgtggcg ttcgccgacg ccgacctgga tgcgacggtg
     3181 aagcaggcgg tgaaccagtt cgacaacgcc gggcaagtct gcctggccgg gactcggctg
     3241 atcgtcgagc gctcgatgcg cgacgcgttc ctcgaacgct tcctcgaggc cgccggcgcg
     3301 atccgccagg gcgaccctcg cgaggagacc accgacatcg ggccgcagat cacgcgcgag
     3361 cacttcgagc gggtcgacgg gttcgtccaa cgcgcgaagg ccgacggcgc cgaggtgctg
     3421 ctgggcggcg gcccgaacga ggagctgggc ggcctgttct atcggccgac gctgttcggc
     3481 gacgtgccga agggagcaga ggtgctccgc aaggaggtgt tcgggccggt cctcacgctc
     3541 cagacgttcg aggacgagga ggaggcgatc gcgctcgcga acgagacgga ctacgggctg
     3601 gccgcgatcc tgtacacggg cgaccgagag cgcgccggac gggtctccga gcggctggtc
     3661 gccggcacgg tctgggtgaa ctgcttcttc gttcgcgacc tccgggcgcc gttcggcggg
     3721 gcgaagtcgt ccggagtcgg acgcgagggc ggcgtctaca gcttcgactt ctacgcagac
     3781 gtgaagaacg tctgctcggc cccctggcct caaggagcgg agcgggaggc catctgacac
     3841 gggaggactg aagatgggtg agatcgtcgg cgcggccctc gtggcccacg tccccacgat
     3901 catgctgccg gaggaggtcc ggctcgagat caacgagggc aaggagatca cgctcgtccc
     3961 cgggctgcac cgcatcaagg ccgaatgcct cgaccggctg caaccggacg ccgtgatcgt
     4021 gttcgacacg cactgggagt ccacgttcga gcacatcgtc acgagccacg accgccggca
     4081 ggggaagttc acgagccacg agctccctcg gggcatgaag cagatcccgt acgacatgcc
     4141 gggcgacccc gagctcgcga acctgatcgc cgcgcaggcc gaggggcgcg acgactgctg
     4201 gatcctggcc aacgacgatc cgtacctccc gctgttctac ggcacggtca acatctggac
     4261 gttcctcggc gatccgaaca cggcctggtt gagcgtcgcg ctgaaccagt gctgcgacac
     4321 gaacgacttc ctcctgttcg gggagctgat cggcaaggcg atcgagcaga gcgaccgacg
     4381 ggtggtgctg ctcgcctctg gcggcatgac ccaccggttc ttcaacttca aggacctgcg
     4441 caagcgggag agccagaagg ccccggacaa tatctacgac cgcgcctact acgacgccga
     4501 catgggcgtg ctccagaagt tcgagaaggg cgaccacgcc ggcgtgatcg acgggatgcc
     4561 cgagtaccgc aaggcctatc cggaggggca cttcggccac tacctgatga tggccgcggc
     4621 gctcggtgga cgggattgca acgcaacggg ggagctgttc agcgagtacg aggcctcggt
     4681 aggcacgggc caggtgcacg tgtggttcga gcggcccgag gggggctgga cggcgtagcc
     4741 ccgccggggt aggaagcacc agcagatccg ttcatcggga gggggggtcc gtggaaggcg
     4801 aacgaccgtt ggtggtgacc gaggagtacg agcgcctcgg cgggaagaag ctctcgctga
     4861 tcgacgtgct cgcgcagtcg gtcggcttca tgggtccggt gttcagcgcc gcgttcatca
     4921 tcccgctgat catcggcgtg aacgcctcgg gtcgaggcgc cgggacgtcc gccgcgctcg
     4981 ccgtgctgct ggcagcgatc ggggtgttcg ccctcggctg gatcgtggcg cagtacgcca
     5041 agcgcatcca cgccgcggga tcgctctatg actacgtctc gaacggcctc ggcaagacgt
     5101 ttggtgccgc gtccggatgg ctctactacg gcgggacgat catcctcacg acgggactcg
     5161 gcctgctgat cggcgggtac gtgcacgaca atttgctgcc gaccctggag atcgacctcg
     5221 acctgccgat ctggctgtgg gacacgatct tcgcgttggg gttgttcgcg gtcctgtact
     5281 tcggcgtgca gatctccacg cgcgtgcagc tcacgctcgc gctgatctcg atcgcggtgg
     5341 tgctgatctt cttcatcacc gtgatcgcgg acctaggtgg ggacaacgac atcgcgaagg
     5401 cgttcgaccc ctcgcccgcc ggtgggttca gcgggatcct gttcggggtg ttgtacgggg
     5461 tactcatctt cgtcgggttc gagactgcgg cgaacctggc cgaggagacg gcggagccga
     5521 aacgctcgat cccgcgggcg gtgctcgggg ccgtggcgat cgtctcgatc ttctacctga
     5581 tcgcggccta cgtcgaggtc gcggggttcg ggttcgacct cgcggtgatc acgagcccag
     5641 aggtggccgg cgctcccctg ttcgcgctcg gtgctccacc aggccccccg gggtcggagt
     5701 tctggctgaa gatcctgctg gtggtcgtgt tcctcgacat gctagcggtg tacgtcgggg
     5761 cggcggtcgc gtcgacccgc ggcgtgttcg cgatggctcg ggatcggcgc ctgccggggg
     5821 gtctggcggc ggtgtccaag cgctacggga cgccgatcgg ggcgatcctg ctgctcatcg
     5881 cgatccaggc ggtgctgatc gtggtgtccg aggcgaacga tacgctcctc gcgctgccgg
     5941 gactcccgca ctacttctcc gtcttcgtgt ggtgtgcgac gttcgggggc ttcgctctcc
     6001 tggtcgtcta cttcctcatg tcgatcgggg ccttccgggg cgtgggcggc gacacggcga
     6061 ccgtggggat catcgtggcg gcactggtgg ggatcgcgat cacggcggcg gcgatcttcg
     6121 ggtcgttcta caaggtgacg agcccgacgc tgctggcgcc gtggtcggcg gtgatctggt
     6181 tcgcgatcgg cctggtgtac atggccctgg tcaagggacg cgagccggcg agcgacgcgc
     6241 tggcggatct gcgcacgagc gcgtgatgcc ggagggggga ggcggggcca tccggccccg
     6301 cctggtcctc atcctctccg agaactggac gctcgtcccc gcgcgcgacc tcggcgcgct
     6361 cgttcggatc gcggtcgatg ccgaggaggc cgggttcgac gccgcgatgg tgagcgagca
     6421 cgtcgtcctc ggtcgcggtg cggacgcgaa cggcgtgccc cagaacccgc gcgactacgc
     6481 ccttccgggg aaccaggacc cgtcgacgcc gtggcccgac gcgctgaccc tgctgagctc
     6541 ggtcgcgtcc gcgacgacga cgctgcgcct cgtggcgagc tcgatcatcg tcccgctccg
     6601 gcacccgttg ctgctcgcca agcagatggc gaccttggat ctcctgtccc ggggacgcct
     6661 ggtcgtccag ccgacggtga gctggcaccg acccgagtac gaggccctcg gggtgccctt
     6721 cgagcggcgt ggggaactcc tcgacgacca cctcgcggct tggcgcgtcc tctggcggga
     6781 cacgccggcg tcgttcgagg gcacgcacta tcggttctcg gacgtgaccc tcgagccgaa
     6841 gccgcaccgg cccgggggac cggtgctgtg gttcggtggc gagaccgtgc acccgcgtct
     6901 cctacggcgg ctggtcgaat acggcagcgg attcaacccg ctcggccggc cgagcaacga
     6961 ggacctcgag cggctgcttt cggcgatggc ggaggcgggg cgggagatgt ccgagatcga
     7021 gatggtaggc gggacgcggg gaaggttcga ggacgccgag agcgtggccg atctcgggga
     7081 ggcgctctcg tcgatccctg aacaactcga acgcgggttc accacgatct gcatcaagcc
     7141 gtcgcagttc atcgacgatc ccgacgggat cgggaccttc tgccgcgagg tcgtcgagcg
     7201 cgtcgacgcg ctaccacctc ctcagatgac gtcgaaataa cgacgtcgct cccactcacc
     7261 gaccgctccg ggcgccagga actgctccca ttcccaccgg ccggcctccg cgtagtgcgc
     7321 gaccacgtcc ttgccgaagg cttccgccga gaacccgccg tcgcggaagc gttcgaccgc
     7381 ctcgccgagc gtggcaggca gcggcgcgac cgggcgttcg tagtcgctgc ccatcagcgg
     7441 ctccccgggt tccgcatcgg tctcgatccc gtcgcgcgcc gacgcgagca gcgccgcgat
     7501 cgcgagatac ggatccacgt ccgcgccggg cacgcgccac tcgagcctgg tggacgcggg
     7561 cgattcggtg agcacccggc acgagaccat ccggctgtcg aacccccacg acagcccgtt
     7621 ccccgagaac tcgccgctcg tcgtgcgccg gtacgagttc accgtcggcg cgtagaagag
     7681 catgagctcc ggccctcgct gcaggacgcc gccgatcgcg tggcggagcg tcgacgagac
     7741 ccccccggcg tcctcctcgt cgtggaagac gttccggccg tccccgtcga ccagcgacag
     7801 gtgcaggtgg cacgacgagc cggtcgtgtc ggcgaacggc cgcgccatga acgtgaccgc
     7861 catcccagcg tccgcggcca gcgtgcgcat cgccagcttg aagagcaggt gccggtcggc
     7921 catctcgagc gcgtgctggt actcgaggtt gatctcccac tggcccagcc cccattcgac
     7981 ctggctggtc cacgggccga gctcggaggc ggcgagcgcg tcccgcaccc ccgagaagaa
     8041 gcgctcgtac cggtcgcccg cctggatcgt gtagtcggcc ctcgcgagcg tggtgggggg
     8101 aagcgtctcg tagccgttgg cgcgaccctc gtcgtagctg ccgtggtaca gggcgaactc
     8161 gagctcgctc gcgacctgcg cggtgaggcc ggtggccacc aggcgctcga tctgccgccg
     8221 caggatcgtg cgcggagcca ccggcgcgag ctcgtccgaa cgcatctcga cgaaatcggc
     8281 gaggcagatc gccgtgcgtt cgagccatgc ggtcggtcgc agcgtcccga gatccggcac
     8341 gagccggacg tcgtgccatc cggtgtggtg cccggtgtac tcctggaccg gccccggcca
     8401 ctggtcgacg tcccagccga ggacgcacga gctgagcggg atcccctctt ggacgacccg
     8461 gcggaacacg tcgaccgaga cgcgcttgcc gaccggccgg cccgtcacgt cggccgacgt
     8521 cacgaccacg gtcgcgaagt cgtccaggtc cgacaggtcg agcgtcaccg ggcgagccat
     8581 ccaccgtcga cgggcagcat ggcgccggtg atgtacgagg cgtcgtcgga gagcaggaac
     8641 gagatggccg cggcgacgtc ctcgggttcg cccacgcgcg ggaccaggag ccggtccttc
     8701 atggatgccc atgcctcggg cgaacggatg ttcccgccgg ggatgaacga ggtcgcgatc
     8761 gccccgggct cgaccgcgtt cacccggatg ttgcggtcgg cgagctcgac cgcgagcgcc
     8821 ttcgtgagca tcttcacgcc gcccttcgac gcgttgtagt gcacctgtgg gtggccggtg
     8881 gaggcgacca cgacctcgga ctcgatcgtc gcgaggttca cgatcgcgcc acctccctca
     8941 cccgcgatca cgggtgcgat cgcctgcgtc acgagataca tgcccttgag gttggtgtcc
     9001 aggaccaggt cccactcctc ctcggcgagc tcgtcgagcg gcgacatccg gacgaggccg
     9061 gcgttgttca cgaggtagtc gatatgcccg aacgcgttca ccgcgccgtc gcgagcgctc
     9121 tcgatctggg atcggtccct tacgtcggcc tccaacgcca cgccggtccc accaccgtcg
     9181 gcgatcgccg cgacggtctc ctcggcggcg tcgagccgga cgtcgacgac cgcgaccgcg
     9241 gcgccctcgg aggcgagacg gagcgcggtc gcgcgaccga tacccgagcc cgccccggtg
     9301 atcagcgcga cccgtcccga gtggtcgaag ctcatggaac ccccaccgaa gccgggcggg
     9361 cccttcggaa cgccatgtcg ggggagtcga tgtcctcctg ggcgtcccca cggtcgacat
     9421 cccggtcctg tccgaggtcc ggaagccaga ccaggagctc gcccccctgg agcagaaggc
     9481 cgatgccggc gctgtaggca tcgaaccgtt gccggagcgg tgtcggctct gtaacgaaca
     9541 ttgcgaagct ccgaccggag ccctcgaccc gcccggacca gtgcccccat cggaacgatc
     9601 cgcccttccg gatgacgatg tcgctccgat tgtcgggaac cgccccacga ggaaggagct
     9661 ccggcgcgac tcccgtgatc gatgcgaatc gccaggttcc ggcgatcggc gtgacatcga
     9721 gcgcggtggt ggcgggggac ggtgacccgg acgcgcgcga tccgcctgac ccgtcgccct
     9781 cgcgggccgg agtggtcgtg gtgctccgag atcgcatgag cagataacta cccgagacaa
     9841 gcagcaggac caccgaaacc gccgttggag cgcgccacgc ggtgcccttc gacccggtag
     9901 gccttccggg ttcgaggctc atggacgctc ctgttgatac gcgacctcgg tgaggtccat
     9961 cgcgaggccg ccgccgtcgg ccaccagggc ggttcccgtc acgatcgagg actcgtcgct
    10021 cgcgaggaag aggcagcacg cggcgatctc gtcggcggtg gccggacggc ggagcggcgt
    10081 gtgccgggtg acgaggtcgt acgcctcctc gagggagatc ccgcgctccg tcatcagatc
    10141 ctgcatcgca cggtcgccca tcggggtcac cacccacccc ggacagagcg cgttcgcccg
    10201 gacgccgcga ggtccgtagt cgacggcgat cgagcgtgcg agcccgttca tggccgcttt
    10261 ggacgtgccg tacgctgcgc tctcggtgcc gctcacgaac gcgttcacgc tcgagaccag
    10321 gacgatcgag ccgcccccgc gctcgatcag cgtggggatc gcggcccgag cgagccgaaa
    10381 cgcgccggtc acgttgacgt cgagcgtccg gtcccagcgc tcgtcgtcga cgtccagggc
    10441 ggccccgccg aacccgatac cggcgttggc gacaacgatg tcgagcccgc cgaacgcctc
    10501 gaccgcggca gcgaccgcct cgcggacgtg gtcctcgtcc gccgtgtcgc cggcgacgac
    10561 ccgacacccg gtctcggcgg cgacctcctc gagcggttcg gcgcggcggc cggtgacgac
    10621 caccttcgcg ccctcgcgcg cgaacagccg agccgcggcc gccccgatcc cggtcccgcc
    10681 gccgctcacg atcgccacct tgccctcgag ccgcatctcg
//
LOCUS       DATBRK010000181         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_28646, whole genome shotgun sequence.
ACCESSION   DATBRK010000181 DATBRK010000000
VERSION     DATBRK010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28646"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VIH70_05475"
     CDS             <1..535
                     /locus_tag="VIH70_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEY8115846.1"
                     /translation="VRNVVTELRGEKIDVVPWSADPATFVANALQPAKVREARVDPET
                     QTALVIVPDYQLSLAIGREGQNARLAARLTGWRIDIKSETQFADEQRGGPRPVVGAVA
                     AGAAKPSPTSAPSAAEAGPAEGEPAGTISEGGSGSPDDRADASAADDEPAATTGDGAG
                     ETEVVDVAEPAEEKAEA"
     gene            538..810
                     /locus_tag="VIH70_05480"
     CDS             538..810
                     /locus_tag="VIH70_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010533992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEY8115847.1"
                     /translation="MAAHVPVRTCVGCRERAAKAELLRLVVAAGGSVRVDPGGSGPGR
                     GAYVHRRRSCVEAALSQGALARGLRTRLDEHGAATLRADIEGELDR"
     gene            807..>2522
                     /gene="infB"
                     /locus_tag="VIH70_05485"
     CDS             807..>2522
                     /gene="infB"
                     /locus_tag="VIH70_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY8115848.1"
                     /translation="MRVHELAKELGVASKELLASLKEMGIDGLTASSSVPDQAVPRLR
                     ASGGKAVPEAKIKEAVEEPLPKSRARKRKEVLEPETPPAPKPAPVAEAEPVTPAAEAA
                     PSVEAPPPPEPVGPSLPALMLVHGSTPQDVADKSGRGVAEIVKILFSLGEMATASQSL
                     SDDALGLLAAELGFEAEIVGVEEEEEPEEEIDESRLEPRAPVVTIMGHVDHGKTLLLD
                     AIRRTDVVSQEFGGITQHIGAYQVHTNGREVTFIDTPGHEAFTAMRARGAQVTDIAVL
                     VVAADDGVKPQTIEALDHARAAGVQVLVAVNKIDKPEADPTRVRQQLVEQGLVPSEWG
                     GTVEFVDVSAKARTNLDNLLDTILLVADLEELKGDPVGRARGVVLEAHLDKGRGPVAS
                     VLVQRGTLDVGDTVVSGTSWAKIRAMLDENGQQVKQAGPSKPVQILGWSATPNAGDDI
                     REVEDERGARHIVQEREAKVRAAELVTTRPPTLQELMAQAERDEVPDLNLIVKADVQG
                     SIGALVDALAKLPQDEVRINVIRSAAGAITESDINLALTSRAIVVGFNVRPDVQARDL
                     AEKEGV"
BASE COUNT          413 a          838 c          963 g          308 t
ORIGIN      
        1 tgtccgcaac gtggtgaccg agctgcgtgg cgagaagatc gacgtcgtgc cctggagcgc
       61 ggaccccgcg accttcgtcg cgaacgcgct gcagccggcg aaggttcggg aggcgcgcgt
      121 ggaccccgag acccagaccg cgctcgtcat cgtcccggat taccagctct cgttggcgat
      181 cgggcgagag gggcagaacg cgcggctcgc cgctcggctc accgggtggc ggatcgacat
      241 caagagcgag acgcagttcg ccgacgagca gcgaggaggg ccgcgtccgg tggtcggggc
      301 ggtggcggcg ggtgcggcga agccttcgcc cacatcggcg ccttcggccg ccgaggccgg
      361 gcccgccgag ggcgagcccg ccggaaccat ctccgagggc gggtcgggct ctcccgacga
      421 cagagcggac gcaagcgccg ccgacgatga accggccgcg acaaccggcg atggcgcggg
      481 ggagaccgag gtggtcgacg tggccgagcc ggcggaggag aaggcggagg cgtagccttg
      541 gcggcgcacg tgccggtgcg gacgtgcgtc gggtgccggg agcgcgcggc caaggcggaa
      601 ctgctgcgac tggtcgttgc ggcgggaggc tcggtccgcg tggacccggg cggttcggga
      661 ccgggcaggg gagcgtacgt gcatcgccgg cgttcgtgcg tggaggccgc cctgtcgcag
      721 ggagcgctcg cgcgcgggct ccggacccgc ctggacgagc atggagccgc tacccttagg
      781 gcggatatcg agggggaact ggatcgttga gggttcacga acttgccaag gagctcgggg
      841 tcgcgagcaa ggagctgctc gcgtcgctga aggagatggg gatcgacggc ctcacggcct
      901 cctcttcggt ccccgaccag gccgtgcccc ggctccgggc gtcggggggc aaggccgtcc
      961 ccgaggccaa gatcaaggag gccgtcgagg aaccgctccc caagtcgcgg gcgaggaaga
     1021 ggaaggaggt cctcgagccc gagacgccgc cggccccgaa gccggccccg gtcgccgagg
     1081 ccgagcccgt aacgcccgcc gccgaggccg cccccagcgt cgaggccccg ccgccccccg
     1141 aacccgtagg cccctcgctg ccggcgttga tgctggtcca cgggtcgacc ccccaagacg
     1201 tggcggacaa gtcgggcaga ggcgtcgccg agatcgtcaa gatcctgttc tcgctcggcg
     1261 agatggcaac ggcgtcgcag tcgttgtccg acgacgcgct cggtctgctc gccgccgagc
     1321 tcgggttcga ggccgagatc gtcggggtcg aggaggagga ggagccggaa gaggagatcg
     1381 acgagagccg gctcgagccc agggcgcccg tcgtaacgat catggggcac gtcgaccatg
     1441 ggaagaccct gctgctggat gcgatccgga ggaccgacgt cgtctcccag gagttcggcg
     1501 ggatcacgca gcacatcggc gcgtaccagg tgcacacgaa cggacgcgag gtcaccttca
     1561 tcgacacccc gggtcacgag gcgttcacgg cgatgcgtgc ccggggcgcg caggtgaccg
     1621 acatcgccgt cctcgtggtc gccgccgacg acggtgtgaa gccgcaaacg atcgaggcgc
     1681 tcgaccacgc gagggccgcc ggcgtccagg tcctcgtcgc cgtgaacaag atcgacaagc
     1741 cggaggcgga cccaactcga gttcggcagc agctcgtgga gcaggggctc gtcccgtcgg
     1801 agtggggcgg gacggtcgag ttcgtcgacg tctccgccaa ggcgcggacc aacctggaca
     1861 acctgctcga cacgatcctg cttgtggccg atctcgagga gctcaagggc gacccggtcg
     1921 gtcgcgcccg cggggtcgtg ctcgaggcgc acctggacaa gggccgcgga cccgtcgcga
     1981 gcgtgctcgt ccagcggggc acgctcgacg tcggcgacac ggtcgtctcg ggcacgtcat
     2041 gggcgaagat ccgcgcgatg ctcgatgaga acgggcagca ggtgaagcag gcgggtccca
     2101 gcaagccggt gcagatcctc ggctggtccg caaccccgaa cgcgggcgac gacatccgcg
     2161 aggtcgagga cgaacgcggg gcgcgccaca tcgtgcagga acgcgaagcc aaggtccgcg
     2221 cggccgagct ggtgaccacg cgcccgccca cccttcagga actgatggcg caggccgagc
     2281 gcgacgaggt ccccgacctc aatctgatcg tgaaggcgga cgtgcagggc tcgatcgggg
     2341 cgctcgtcga cgcgctcgcc aagctgccgc aggacgaggt ccggatcaac gtgatccgga
     2401 gcgccgccgg cgcgatcacc gagagcgaca tcaacctggc gctgacctcg agggcgatcg
     2461 tcgtcgggtt caacgtccgt cccgacgtgc aggcgcgcga cctcgccgag aaggaaggcg
     2521 tc
//
LOCUS       DATBRK010000182         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2340575, whole genome shotgun sequence.
ACCESSION   DATBRK010000182 DATBRK010000000
VERSION     DATBRK010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340575"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VIH70_05490"
     CDS             complement(<1..853)
                     /locus_tag="VIH70_05490"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEY8115849.1"
                     /translation="MAAGATVAVIAGGALGYFTLFPQQAPAFVRDTLQGVGLGAEPEA
                     TCPLTGAPVHGSEVPARPALAVKIENAPEARPQAALNEADVVVEEPVEGGYTRFIAVF
                     QCGDSDRVGPVRSGRTTDPDYLRQLGPAVFGYAGGRDVVKREVPKVGLVDVNYIVAAG
                     AYTRDDSRAAPHNLYTTTDALWRAGRSKEEAPDPLFTYAQTWDGAAKRLRSVHLPYSG
                     VSDVYWHWNGAQGAWLRSHGDVPHVLEGNERVSATNVVIQVVSVTDSDFLDSAGNPSP
                     EVDLTGSG"
     gene            complement(1056..1964)
                     /locus_tag="VIH70_05495"
     CDS             complement(1056..1964)
                     /locus_tag="VIH70_05495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY8115850.1"
                     /translation="MYEQIASNKRKTVLLIVGAIVLLGAVGYVLGIWYGSGPAGLIGA
                     VALAIVLSLASFFGGDRLVLASTRAREVTPQEQARLHNIVEGLSIAAGIPKPRVYVIP
                     EQAPNAFATGRNPEHASVAVTEGLLATMNRVELEGVIGHELSHVVDRDILVGTVVATL
                     VGAVVLISEFFLRSWWWGGFRGRRGGDRNGGGIEAIIFAIGLVLLVLAPIIGQIIRLA
                     VSRQREFLADAQGALLTRYPPGLASALRKIGAASGIPMRSANNATAHLWLNQPSRVHG
                     EGMGPLERLFSTHPPIEERIRRLEEM"
     gene            complement(1968..>2363)
                     /locus_tag="VIH70_05500"
     CDS             complement(1968..>2363)
                     /locus_tag="VIH70_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY8115851.1"
                     /translation="TTVQGYATHERELFQLVTEARTQAIDANAVPDQAQAENQITRGI
                     RQLFAVAENYPDLKANQNFLALQEELTGTESKIAFARQFYNDQVMLLNTLIGSFPSNV
                     VARAFGFGPREFFDIEDPVRAPSQVEFPG"
BASE COUNT          336 a          847 c          826 g          354 t
ORIGIN      
        1 tgcccgaacc cgtcaggtcg acctcgggcg aggggttgcc ggcggaatcg aggaagtcgc
       61 tgtcggtcac cgagacgacc tggatcacga cgttggtggc cgagacgcgc tcgttgccct
      121 cgagcacgtg cgggacgtcg ccgtgcgaac gcaaccacgc gccctgggcg ccgttccagt
      181 gccagtagac gtcggacacg cccgagtacg gcaagtgcac cgaccggagc cgcttcgcgg
      241 ccccgtccca cgtctgggcg tacgtgaaca gcgggtcggg cgcctcctcc ttcgacctcc
      301 cggcgcgcca gagcgcgtcg gtcgtggtgt agaggttgtg cggtgctgcc cgggagtcat
      361 cgcgcgtgta cgcgccggcg gccacgatgt agttcacgtc gaccagcccg acctttggca
      421 cctctcgctt caccacgtcg cgcccgcccg cgtagccgaa caccgccggc ccgagctggc
      481 ggaggtagtc cgggtccgtc gtccggccgc tgcggaccgg ccccacacgg tccgagtcgc
      541 cgcactggaa cacggcgatg aatctcgtgt acccgccctc gaccggttcc tcgacgacca
      601 cgtcggcctc gttgagcgcg gcctgcggtc gcgcctccgg ggcgttctcg atcttgaccg
      661 cgagcgccgg ccgggccggg acctcgctcc cgtgaacggg cgcgccggtc agcgggcacg
      721 tggcctccgg ctcggctccc aggccgacgc cctgcaacgt gtcgcgcacg aacgccgggg
      781 cctgctgcgg gaacagcgtg aagtagccga gcgccccgcc cgcgatgacg gcgaccgtcg
      841 ctccggcggc cacgagcttg ccgcgacggg tgaaggttcg gcgggtttgg ggcgagttcg
      901 tgcgatggct tccggacatt cgggactccc agggggtcgc gggcgtatgg gggatgtccc
      961 cggccccgtt ccgggagccg gtcgtgacag cgggcgcaga tgctacccgg cgtccggagg
     1021 cgtctcaacg acccggaccg atctcgtcgg gatcgtcaca tctcctcgag ccgccggatc
     1081 cgctcctcga tcggcgggtg cgtgctgaac agcctctcga gcgggcccat gccctcgcca
     1141 tggacccgcg acggctggtt cagccacagg tgcgccgtcg cgttgttcgc cgaccgcatc
     1201 gggatccccg atgcggcccc gatctttcgg agcgcgctcg cgaggcccgg cgggtaccgc
     1261 gtcaacagcg ctccctgcgc gtcggccagg aactcgcgct gcctcgacac cgccagacgg
     1321 atgatctgcc cgatgatcgg cgcgagcacg agcagcacca gcccgatcgc gaagatgatc
     1381 gcctcgatgc cgccgccgtt ccggtcgccg ccgcggcggc cgcggaagcc gccccaccac
     1441 cacgaccgca ggaagaactc gctgatcagc accacggcgc ccacgagcgt ggccacgacc
     1501 gtcccgacga ggatgtcgcg gtccaccacg tgggagagct cgtgcccgat cacgccctcg
     1561 agctcgacgc ggttcatcgt ggcgagcagc ccctcggtga cggcgacgga ggcgtgctcg
     1621 gggttgcggc cggtcgcgaa cgcgttcggc gcctgctcgg ggatcacgta gaccctcggc
     1681 ttggggatcc cggccgcgat cgagagcccc tcgacgatgt tgtgcaggcg cgcttgctcc
     1741 tgcggcgtca cctcgcgcgc gcgcgtcgag gccaggacga ggcggtcgcc cccgaagaag
     1801 gaggcgagcg acaggacgat cgcgagcgcg accgccccga tgagcccggc ggggccgctc
     1861 ccgtaccaga tgccgaggac gtacccgacc gcgccgagca gcacgatcgc cccgacgatg
     1921 agcaggacgg tcttgcgctt gttcgatgcg atctgctcgt acacggacta cccggggaac
     1981 tcgacctgcg acggcgcgcg gaccggatcc tcgatgtcga agaactcccg cggtccgaac
     2041 ccgaacgccc tggcgacgac gttcgagggg aacgagccga tcagcgtgtt caacagcatg
     2101 acctggtcgt tgtagaactg ccgcgcgaac gcgatcttgg attcggtccc ggtgagctcc
     2161 tcctgcaggg ccaggaagtt ctggttggcc ttcaggtcgg ggtagttctc cgccaccgcg
     2221 aacagctggc ggatcccacg cgtgatctgg ttctcggcct gcgcctggtc cgggaccgcg
     2281 ttcgcgtcga tcgcctgggt tcgcgcctcg gtgacgagct ggaacagctc gcgctcgtgg
     2341 gtcgcgtacc cctggacggt cgt
//
LOCUS       DATBRK010000183         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2484967, whole genome shotgun sequence.
ACCESSION   DATBRK010000183 DATBRK010000000
VERSION     DATBRK010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484967"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VIH70_05505"
     CDS             <1..594
                     /locus_tag="VIH70_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HEY8115852.1"
                     /translation="KLSGTLSRFDTTAIFINQLREKIGVMFGNPETTPGGRALKFYSS
                     VRLDVRKIENLKDGTDVVGSRTRVKVVKNKVAPPFRQCEFDIMYGVGISKEGSLLDVG
                     VDLEIVKKSGAWFTYEGDQLGQGRENARQFLAEHTDIATELERRVREAVGLTTFGAEG
                     DDTPIEVVPDLAETDEQIAPKAAKADKERETTGARAG"
     gene            626..1120
                     /locus_tag="VIH70_05510"
     CDS             626..1120
                     /locus_tag="VIH70_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY8115853.1"
                     /translation="MATKPTVKRPLDCHERALRLLAVRPRARRELESRLRRAGFEAAE
                     VSSELDRLEEVGLLDDEAFARELAEHHLTVRGSGRRAVAGALAAKGVPRETIDVTLAG
                     LVEQDESARALEVAKDRARRLTGLRPEAAYGRLVAFLARRGYDGGVSREAAKAALGLD
                     DRHA"
     gene            1395..>2830
                     /gene="rny"
                     /locus_tag="VIH70_05515"
     CDS             1395..>2830
                     /gene="rny"
                     /locus_tag="VIH70_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY8115854.1"
                     /translation="MVPVVVGIVALALGVAIGFVIRRAVARTQAESAESRAQKVILEA
                     EREAERLTREALVEAKEEIGALRRETEEDVRTRRDEIKRQEDRLTQKEQAVDRKTDEA
                     ERRSEELDDRDRTLALVREQLEHSAVQHRAALEKIARLTASEAKEALRAQVVDEAKRE
                     AMATVREIEERAREEGEERARKIVTIAIQRVASEQTSESTVSVFSLPSEDMKGRIIGR
                     EGRNIRAFEATTGVNLIIDDTPEAVVLSSFDPVRRETARLTLEKLVQDGRIHPARIEE
                     TFERSQKEIDEQIKRAGEEAVLDTGITDMHPEMIRLLGRLQFRTSYGQNVLKHLVESA
                     HIASAIASELGIDPTVPKRGALLHDIGKAVTHEVEGSHAIIGAEIARRMKESPEVVHC
                     IESHHGEVEQRTIDAVIAQIADGISGGRPGARRESLETYIKRLERLEEICTSYPGVGK
                     VYAMQAGREVRVMVKPEDIDDLSAQVMAR"
BASE COUNT          494 a          927 c         1060 g          349 t
ORIGIN      
        1 aagctctcgg gcacgctctc gcggttcgac accacggcga tcttcatcaa ccagctccgc
       61 gagaagatcg gcgtgatgtt cggcaacccc gagacgaccc ccggcggccg cgcgctgaag
      121 ttctactcct cggtccggct ggacgtgcgc aagatcgaga acctgaagga cgggaccgac
      181 gtggtgggat cgcggacgcg cgtcaaggtc gtgaagaaca aggtcgcgcc ccccttccgc
      241 cagtgcgagt tcgacatcat gtacggcgtc ggcatctcca aggaagggag cctcctcgac
      301 gtcggcgtcg acctcgagat cgtcaagaag agcggggcgt ggttcacgta cgagggcgat
      361 cagctcgggc agggtcgcga gaacgcgcgc cagttcctgg ccgagcacac ggacatcgcg
      421 accgagctcg aacgccgggt gcgcgaggcg gtcggcctga cgacgttcgg cgccgagggc
      481 gacgacacgc cgatcgaggt cgtcccggac ctcgcggaaa ccgacgagca gattgctccg
      541 aaggcggcca aggccgacaa ggagcgtgag acaacggggg cacgagcggg ttaggggaag
      601 gcccgctcgc ccccggcgtc ctcacatggc gaccaagccg accgtcaagc ggccgctcga
      661 ctgccacgag cgggcgctcc ggctgctcgc ggtccgtccg agagcgcggc gcgagctcga
      721 gtcgcggctg cgccgggcgg ggttcgaggc ggccgaggtg tcgtcggagc tcgaccggct
      781 ggaggaggtc gggctgctcg acgacgaggc cttcgcgcgc gagctggccg agcaccacct
      841 gacggtccgc gggagcgggc gacgggcggt ggcgggcgcg ctcgcggcca agggcgtgcc
      901 gcgggagacg atcgacgtga ccctggcggg cctggtggaa caggacgaga gcgcccgagc
      961 gctggaggtg gccaaggacc gggcgcggcg cctcacgggc ctgcggccgg aggcggcgta
     1021 cgggcgcctg gtggcgttcc tcgcccggcg cggctacgac ggcggcgtct cccgggaggc
     1081 cgccaaggcg gccctggggc tcgacgaccg ccacgcctga gcgcccgggt ccggcgccgc
     1141 gaggcggcca cccttgcctc taccgagcgc acgccgtacg ctctgttcct acgaggttca
     1201 tccgttggtc aacgttcatc cgtgcctgaa accttgagaa accagacctc acagggagca
     1261 caccggatcg gagggcgagc agggccgccg gccccggcat cgcgctgatc cgcatccttt
     1321 cggacgcacg gccgaacagt gcccgatggg cctcggttcg gctgttccgt gtctacgagc
     1381 gaggcggtga accgatggta ccggtggtgg tggggatcgt tgccctggca ctgggcgtgg
     1441 cgatcggctt cgtgatccga cgagcggtcg ccaggacgca ggccgagagc gccgagagca
     1501 gagcgcagaa ggtgatcctt gaggctgagc gcgaggccga gcgcctcacg cgcgaggccc
     1561 tggtggaagc gaaggaagag atcggcgcgc tccggcgcga gaccgaggag gacgttcgca
     1621 cccggcgaga cgagatcaag cgccaggagg accgcctcac ccagaaggag caggccgtcg
     1681 accgcaagac cgacgaggcg gaacggcgtt cggaggagct ggacgatcgg gaccgaacgc
     1741 tcgcgctggt ccgcgagcag ctcgagcact cggcggtcca gcatcgcgcc gcgctcgaga
     1801 agatcgcgcg gctcaccgcg tccgaggcga aggaggcgct ccgcgcgcag gtggtggacg
     1861 aggccaagcg cgaggcgatg gccacggtcc gcgagatcga ggaacgcgcg cgcgaggagg
     1921 gggaggagcg cgctcgcaag atcgtgacga tcgcgatcca gcgcgtcgcc tccgaacaga
     1981 cgagcgagtc gaccgtctcg gtgttctcgc tcccgagcga ggacatgaag gggcgcatca
     2041 tcggtcgtga gggccggaac atccgcgcgt tcgaggcgac gaccggggtc aacctgatca
     2101 tcgacgacac gcccgaggcc gtggtgctgt cgagcttcga ccccgtgcgg cgcgagacgg
     2161 cgcggctcac gctcgagaag ctcgtgcagg atgggcggat ccacccggcg cggatcgagg
     2221 agacgttcga gcgatcgcag aaggagatcg acgagcagat caagcgcgcg ggcgaggagg
     2281 ccgtcctcga taccgggatc accgacatgc accccgagat gatccggctg ctcgggcggc
     2341 tgcagttccg gacctcctac ggccagaacg tcctcaagca tctagtcgag tcggcccaca
     2401 tcgcctcggc gatcgcatcc gagctcggga tcgacccgac cgtccccaaa cgcggggcgc
     2461 tgctgcacga catcggcaag gcggtgacgc acgaggtcga gggctcgcac gcgatcatcg
     2521 gcgccgagat cgcccggcgc atgaaggagt cgcccgaggt ggttcactgc atcgagtccc
     2581 accacggcga ggtcgagcag cggacgatcg acgccgtgat cgcccagatc gccgacggga
     2641 tctcgggcgg ccgcccgggg gcccgccgcg agagcctcga gacctacatc aagcgcctcg
     2701 agcgcctcga ggagatctgc acgagctacc ccggcgtggg gaaggtctac gcgatgcagg
     2761 ccggccgcga ggtccgcgtg atggtcaagc ccgaggacat cgacgacctc tcggcgcagg
     2821 tgatggcgcg
//
LOCUS       DATBRK010000184         9209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_748853, whole genome shotgun sequence.
ACCESSION   DATBRK010000184 DATBRK010000000
VERSION     DATBRK010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748853"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..657)
                     /gene="dtd"
                     /locus_tag="VIH70_05520"
     CDS             complement(214..657)
                     /gene="dtd"
                     /locus_tag="VIH70_05520"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY8115855.1"
                     /translation="MRVLLQRVHRASVTVRDELLSEIGPGLLLFVGVGADDGFGEYDR
                     LAEKVFHLRVFEDEHGKMNRSLSDVGGEILIVPQFTLYGDVRRGRRPSWDGAAPPEIA
                     EDRVEAFAKALEARGATVRRGAFREHMQVELVNDGPVTLWIDSAG"
     gene            complement(654..2909)
                     /locus_tag="VIH70_05525"
     CDS             complement(654..2909)
                     /locus_tag="VIH70_05525"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004990594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY8115856.1"
                     /translation="METGEEVRTERRGGILSRLRSGEPEAHEGIEALVRTVKGYNAKA
                     DLKEIERAYRFAAEHHEGQKRLSGEDFIEHPLAVAQILADLGLDTTTLEAALLHDTVE
                     DTDVSVADLETEFGPEVSRIVDGLTKLDALSFRSREHEQAENVRKMIVAMAGDIRVLL
                     IKLADRLHNMRTLSALPKDKQRRIATATLEIYAPLAHRLGVQQVKWELEDLSFKTLHP
                     GPYHEIASLVEKRRGERQQYVEGVLEAARVRIREAGLKAEVGGRPKHLYSIYEKMVVG
                     GKEFNEIYDLAGIRVQVDSVRDVYAALGAIHSLWKPVPGRFKDYIAMPKSNMYQSLHT
                     TVVGPQGRPIEVQIRTKEMHRTAEYGIAAHWRYKEGRDGKKAKEAADLAWLGQMLEWL
                     KDMADPREFMEGLKIDLYGGQVFCFTPKGDVMNLPAGATPVDFAYAIHTEVGHRTIGA
                     KVNGKLVPLDYELRTGDTVEILTSKAQGEGPSQDWLRWVKTPRARNKIRQWFSRERRE
                     DALEDGREQLQRLMRKQNVPLKRLATEDALEQLADEMKFPSLEALYVAIGEGQVSPQS
                     IVARLARSVQGSPEEDVTELPLARPVHLGQPSSTDVTSGVVVPGSEDVWVRLARCCTP
                     VPGDDILGFVTRGQGVSVHRTDCPNAKSLISQPERLIDVTWKAGKPTSFVVSVQVEAL
                     DRTRLLSDVATVLSDHHVNILSATSAVGRDRTTMLRFTFELADISHLTEILGAVKKVE
                     NVYDAFRVVPR"
     gene            complement(3014..3535)
                     /locus_tag="VIH70_05530"
     CDS             complement(3014..3535)
                     /locus_tag="VIH70_05530"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY8115857.1"
                     /translation="MDLDQLKALVRDVPDFPQEGVLFKDITPLLADPLAFSTVIDLIV
                     VHFGRGNVDKVVGIEARGFILASPVAYHFGAGFVPVRKVDKLPWETESAEYELEYGTA
                     TLEIHRDAVAPGERVLIVDDVLATGGTSQATAELVERIGGKVVGIACLIELEFLHGRE
                     KLEGHDLYTLLRY"
     gene            complement(3540..4688)
                     /gene="secF"
                     /locus_tag="VIH70_05535"
     CDS             complement(3540..4688)
                     /gene="secF"
                     /locus_tag="VIH70_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY8115858.1"
                     /translation="MSVRRAFDTFRGYRHPEFHIIQRRRRWFILSGSAILISILALVF
                     LGLNYSIDFTGGTLIEYRLTKDVTVEQVRGLLAQDPFDRATAEVQIVGGDQVSIRTTA
                     LTDLTAAERTQLFEALAQQTGISPDDISAQVVGPTWGEQISRQALIGLVVVLFAITLY
                     ITLRFEWKMAIGAMVAMVHDVLITAGVYALTGREVSPPTVIAILTILGFSLYDTVVIF
                     DKVKENTESTALLGKDTYEGVANYSMNQVLMRSVNTSLVVILPILSLLLFGGDTLKDF
                     AFAMLIGVITGAYSSIFVATPILVILKEREPRYRQLRSRLERGSKAERRLRAVPTNGQ
                     EAAEEEPEAAPAAVAAAGGSRQGGRSGQASSRPRPKSKRRPPAKRRRR"
     gene            complement(4685..6709)
                     /gene="secD"
                     /locus_tag="VIH70_05540"
     CDS             complement(4685..6709)
                     /gene="secD"
                     /locus_tag="VIH70_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY8115859.1"
                     /translation="MRKKQRSFWVSLVVVGLLAVGAIALFVTGTRPVLGLDLEGGVSV
                     ILSAPDGTPDTVMNQALENIRNRIDAFGTAEPLLFVTGNTIEVQIPGLARGTIQERPK
                     QQSCIADDKGNVYTCFDTQDAADGALGAASVRPVVSSVCLTDDPANESTPTLFGEDAP
                     CVGTEKDATTVLDAIAVKKQQEEFCLTGTGLATDPCYPTRDEADQALSSIETVTSQSF
                     CIQTEGKETLTSDLGAACTPSQEEAQTLLDGMTIRNADTQFCVVSSAGENLGCFLDRE
                     AAEARLQETGQERLLQVIGETARLEQREVIGVLAPTDPAYASTPLTCGTPAEQATTEC
                     SFEALKDQEVVFQDRDDATRYQLGPVEITGDAIDKATAVYNAGSSTSVGQGWEIRFDL
                     TNEGSGIFSDVTTRLEGKQLAIVVDDQVISAPTVQSPITNGSGVITGSFTEREAKDLA
                     TQLNAGALPVELTTQQVLTVSPTLGDESLQQGLVAAIAGLVLLAAYLLFYYRILAVVA
                     WVGMMIWATLALGLVALAGRSIGYNLTLAGVAGLVISLGVTADSYIVFFERLKDEVHA
                     GRSPRAAVAPAFKRSFRTIVAADLVTAIAAAVLYVTAISSVRGFALTLGVATLLDLFV
                     VYFFKRPAVSLLTRSERIVNWRVMGVKAGLAADRGLVAPDAVPAIVGGSE"
     gene            complement(6729..7451)
                     /locus_tag="VIH70_05545"
     CDS             complement(6729..7451)
                     /locus_tag="VIH70_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEY8115860.1"
                     /translation="MSAEPAPAEGPSEPAEEASQPEGITLEVVQHDEELSSFIAAADR
                     VMEGLGYTEHGFRHANLTARIAFNVLSRAGFDTHTANLACVAGYLHDVGNMISRYAHG
                     QTGGVLVYQALRDRVPSTDLAQIVAAIANHEEDNGYAVSPVAAAVILADKSDVHRSRV
                     RKSGQIEFDIHDRVNFAAEQSFLRVDSVAKTVSLELTIDTKISQVMEYFEIFLGRMQL
                     CRLAAEVLNCRFKLLINGAELA"
     gene            complement(7448..7810)
                     /gene="yajC"
                     /locus_tag="VIH70_05550"
     CDS             complement(7448..7810)
                     /gene="yajC"
                     /locus_tag="VIH70_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017930742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY8115861.1"
                     /translation="MVLGILAQDSQSGSSFIGLALPVLMIVGFYFLLIRPQRNRQRAQ
                     QALVASLEVGDDVMTTGGIFGTIVEIDDDEGTVTVEIAPGTRIRMLRQGISQRFVEDT
                     YEDDGGDENADEGIDREP"
     gene            complement(7898..8962)
                     /gene="ruvB"
                     /locus_tag="VIH70_05555"
     CDS             complement(7898..8962)
                     /gene="ruvB"
                     /locus_tag="VIH70_05555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY8115862.1"
                     /translation="MRRFNASGAGRVTADVTQDERIVDPEVPEKEREFDAALRPRRID
                     EFVGQDRIKEQLALLVDGARARGETVDHLLFSGPPGLGKTTLAGIVSNELGVGFQPTS
                     GPALDRPGDLAAILTNLEEGDVLFVDEIHRMPRAVEEVLYPALEDFKLDVVLGKGPSA
                     RSIRLDLPRFTLVGATTRPGRITLPLRERFGFSPRLDYYSIDDLGAIVRRSAGILGVS
                     IDAEGTHEIARRSRGTPRVANRLLRRVRDFAEVRHDGNVTSDVARAGLELFEVDEEGL
                     DKLDLAILSTVVVKFGGGPVGLSTLSAAVGEETDTIEDVVEPYLLQLGLLQRTPRGRL
                     ATARAYRHLGVSPEGVLPLS"
     gene            complement(8931..>9209)
                     /locus_tag="VIH70_05560"
     CDS             complement(8931..>9209)
                     /locus_tag="VIH70_05560"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY8115863.1"
                     /translation="RRAVSSGDVAALTVVPGVGKKVAQRVVLDLKDKLGAEDVVIIDG
                     PLADVREALLALGLSPQEASEAMTGLTTHGDSSVEDLLREALQRIGRG"
BASE COUNT         1376 a         3252 c         3098 g         1483 t
ORIGIN      
        1 cggaccggct ccccgttcct ggtgacctcg gagacctcga tcacgccgcg cgacgtggcg
       61 acctcgacgc ggcgccccgc cttcgtcgcc tcgatctcgt aggtcagcac gcccgagccc
      121 gagtcgacga cgatctccac ccggtcgccc ttcacgagag gagcctactc ctcggcccgg
      181 acatgccccc gcggcacggc tccgtgagcc gcgtcagccg gcggaatcga tccagagggt
      241 gaccgggccg tcgttcacga gctcgacctg catgtgctcc cggaacgctc cgcgccgcac
      301 cgtcgcgccc cgggcctcca gggccttcgc gaacgcctcg acccggtcct cggcgatctc
      361 gggcggggcg gcgccgtccc acgagggccg ccggccccgg cggacgtcgc cgtagagcgt
      421 gaactgcgga acgatcagga tctcgccccc gacgtccgac agcgagcggt tcatcttgcc
      481 gtgctcgtcc tcgaacaccc ggaggtggaa gaccttctcg gccagccggt cgtactcgcc
      541 gaacccgtcg tcggcgccca cgccgacgaa aagaaggagg ccgggtccga tctcggacag
      601 gagctcgtcg cggaccgtga ccgatgctcg gtggacgcgc tggagcagga ccctcaacgc
      661 ggcacgacgc ggaacgcgtc gtagacgttc tcgaccttct tgacggcccc gaggatctcg
      721 gtgaggtgcg agatgtccgc gagctcgaac gtgaaccgaa gcatcgtggt ccggtcacgg
      781 ccgacggccg aggtcgccga caggatgttc acgtggtgat ccgagagcac cgtggcgacg
      841 tccgagagca ggcgggtgcg gtcgagcgcc tccacctgga ccgagacgac gaacgacgtc
      901 ggcttgccgg ccttccaggt gacgtcgatc agccgctcgg gctgggagat gagcgacttc
      961 gcgttcgggc agtcggtgcg gtggaccgag acgccctgcc cgcgcgtgac gaacccgagg
     1021 atgtcgtccc cgggcaccgg ggtgcagcac cgggcgaggc ggacccacac gtcctcggag
     1081 ccgggcacca ccacccccga ggtcacgtcg gtcgagctcg gctgcccgag gtgcaccggc
     1141 cgcgcgagcg ggagctcggt gacgtcctcc tcgggcgagc cctgcacgct ccgcgccagg
     1201 cgggcgacga tcgattgcgg cgagacctgt ccctcgccga tcgcgacgta cagcgcctcg
     1261 aggctcggga acttcatctc gtccgcgagc tgctcgagcg cgtcctcggt cgcgagacgc
     1321 ttgagtggga cattctgctt gcgcatcaat cgctggagct gctcccggcc gtcctcgagg
     1381 gcgtcctcgc gccgctcgcg actgaaccac tgccggatct tgttacgggc gcgtggcgtc
     1441 ttgacccatc ggagccagtc ctgcgacggt ccctcgccct gcgccttgga ggtcaggatc
     1501 tcgaccgtgt cgccggtccg aagttcgtag tcgagcggga cgagcttgcc gttcaccttg
     1561 gccccgatcg tccggtgccc gacctcggtg tggatcgcgt acgcgaagtc aaccggcgtc
     1621 gcgccggccg gcaggttcat cacgtcgccc ttcggcgtga agcagaagac ctgcccgccg
     1681 tacaggtcga tcttcaggcc ctccatgaac tcgcgtgggt cggccatgtc cttcagccac
     1741 tcgagcatct gaccgagcca cgcaaggtcg gcggcctcct tggccttctt cccgtcgcgc
     1801 ccctccttgt accgccagtg ggcggcgatc ccgtactcgg cggtccggtg catctccttg
     1861 gtgcggatct gcacctcgat cgggcggccc tgcggtccga cgacggtcgt gtgcagcgac
     1921 tgatacatgt tcgacttggg catcgcgatg tagtccttga accgcccggg gacgggcttc
     1981 cacagcgagt ggatcgcgcc gagcgccgcg tagacatcgc gcaccgagtc gacctggact
     2041 cggatcccgg cgaggtcgta gatctcgttg aactccttgc cgccgaccac catcttctcg
     2101 tagatcgagt acaggtgctt cgggcggcca cccacctccg ccttcagccc cgcctcgcgg
     2161 atcctcacgc gggcggcctc gagcacaccc tcgacgtact gctgtcgctc gccgcggcgc
     2221 ttctcgacca ggctcgcgat ctcgtggtat ggcccggggt gcagcgtctt gaacgacagg
     2281 tcctcgagct cccacttgac ctgctgcacg cccagacggt gtgcgagcgg cgcgtagatc
     2341 tcgagcgtcg cggtggcgat ccgccgctgc ttgtccttcg ggagcgcgga gagcgtgcgc
     2401 atgttgtgca gccggtcggc gagcttgatc agcaggacgc ggatgtcgcc cgccatcgcc
     2461 acgatcatct tgcggacgtt ctccgcctgc tcgtgctccc ggctgcggaa cgagagcgcg
     2521 tcgagcttgg tgaggccgtc cacgatccgc gagacctcgg gcccgaactc ggtctccagg
     2581 tccgcgacgc tcacgtcggt gtcctcgacg gtgtcgtgca ggagcgccgc ctccagggtc
     2641 gtcgtgtcca gccccagatc ggccagaatc tgggcgacgg cgagcgggtg ttcgatgaag
     2701 tcctcgcccg acaggcgctt ctggccctcg tggtgctcgg cggcgaaccg gtaggcgcgc
     2761 tcgatctcct tcaggtcggc cttggcgttg tagcccttga ccgttcgaac gagcgcctcg
     2821 atgccctcgt gcgcctcggg ctcacccgag cgcaggcgtg aaaggatgcc gccgcgccgc
     2881 tcggtcctga cctcctcgcc cgtctccacg ctcgcctctc gccccgaaca ccaagtctac
     2941 gccgcgggtg cacgcgaacc gtgcggcggt tcccgatgga ggcaacgagc ggcctcaacg
     3001 acccgaacgg gtctcagtag cgcaggagcg tatacaggtc gtggccctcg agcttctcgc
     3061 ggccgtggag gaactcgagc tcgatcaggc acgcgatccc gacgaccttg ccgccgatcc
     3121 gttcgacgag ttcggcggtc gcctgcgagg tcccgcccgt cgccaggacg tcgtcgacga
     3181 tcagcacccg ttcgccgggt gcgacggcgt cccggtggat ctcgagcgtc gccgtcccgt
     3241 actcgagctc gtactcggcc gactcggtct cccacgggag cttgtccacc tttcggaccg
     3301 gcacgaagcc cgcgccgaag tggtacgcaa ccggcgacgc gaggatgaac ccgcgcgcct
     3361 cgatccccac caccttgtcc acgttgccgc gcccgaagtg cacgacgatc aggtcgatca
     3421 ccgtcgagaa cgcgagcgga tcggccagga gcggcgtgat gtccttgaac aggacgccct
     3481 cctgcgggaa gtcgggcacg tcccggacga gcgctttcag ctgatcgaga tccacggggt
     3541 cacctccgcc ggcgtttggc gggcggtcgt cgcttgctct tcgggcgcgg tcgcgaggac
     3601 gcctgcccgc ttctaccgcc ctgtcgggag ccgccggcgg cggccaccgc cgccggagcg
     3661 gcctccggct cctcctcggc cgcctcctga ccgttcgtcg ggacggctcg caggcggcgc
     3721 tccgccttgc tcccgcgctc gaggcgcgac cggagctgcc ggtacctggg ctcgcgctcc
     3781 ttcaggatga ccaggatcgg cgtggcgacg aagatcgagg agtacgcccc cgtgatgacg
     3841 ccgatcagca tcgcgaacgc gaagtccttc agggtgtcgc cgccgaacag cagcagcgac
     3901 aggatcggca ggatcaccac gagtgacgtg ttgaccgacc gcatcagcac ctggttcatc
     3961 gagtagttcg cgacgccctc gtacgtgtcc ttgcccagca gcgcggtcga ctcggtgttc
     4021 tccttcacct tgtcgaagat caccacggtg tcgtacaggg agaacccgag gatcgtcagg
     4081 atcgcgatca cggtcggcgg cgagacctcg cggccggtga gcgcgtagac acccgccgtg
     4141 atcaggacgt cgtgcaccat ggcgaccatc gcgccgatgg ccatcttcca ttcgaaccgg
     4201 agcgtgatgt atagggtgat cgcgaacagg acgaccacca gcccgatcag ggcctgtcgc
     4261 gagatctgtt ccccccacgt cggccctacc acctgcgcgc tgatgtcgtc cggactgatc
     4321 ccggtctgtt gcgcgagcgc ctcgaacagc tgcgtgcgtt cggcggcggt gaggtcggtc
     4381 agagccgtgg tccggatcga gacctggtcg ccgcccacga tctgcacctc ggccgtcgcc
     4441 cggtcgaacg gatcctgcgc gaggaggccg cgcacctgtt cgacggtcac gtccttggtg
     4501 agccgatact cgatcagcgt gccgcccgtg aagtcgatcg agtagttcag cccgaggaac
     4561 acgagcgcga ggatcgagat caagatcgcc gaacccgaga ggatgaacca ccgacggcgc
     4621 cgctggatga tgtggaactc ggggtgccga tacccgcgga acgtgtcgaa cgcgcgccgc
     4681 acgctcattc cgaccccccc acgatcgccg gcaccgcgtc cggagcgacg agccctcgat
     4741 ccgccgccag ccccgccttg acgcccatca cacgccagtt cacgatccgc tcgctgcgcg
     4801 tgagcaggga cacggccggg cgcttgaaga agtagacgac gaacaggtcg aggagcgtag
     4861 ccacgccaag cgtgagtgcg aacccacgca cggaggagat cgcggtgacg tacaggaccg
     4921 ccgccgcgat cgcggtcacg aggtcggcgg ccacgatcgt gcggaacgac cgcttgaacg
     4981 cgggcgcgac cgcggcacgg ggcgagcgcc cggcgtggac ctcgtccttc agccgttcga
     5041 agaacacgat gtaggagtcg gcggtgacgc ccagggagat gacgagcccc gcgacgccgg
     5101 cgagcgtgag gttgtacccg atcgatctgc ccgcaagtgc cacgagcccg agcgcgaggg
     5161 tcgcccagat catcatcccg acccacgcca cgaccgcgag tatccggtag tagaacagga
     5221 ggtaggccgc cagcaacacg agccccgcga tcgccgcgac gagcccctgc tgcagcgact
     5281 cgtcgcccag ggtcgggctc acggtcagga cctgctgggt ggtgagctcc acgggcagcg
     5341 ccccggcgtt cagctgcgtc gccaggtcct tcgcctccct ctcggtgaag ctgccggtga
     5401 tcacgccgct cccgttggtg atcgggcttt ggacggtcgg tgccgagatg acctggtcgt
     5461 cgacgacgat cgcgagctgc ttaccttcga gtcgggtcgt cacgtccgag aagatgccgg
     5521 acccctcgtt ggtgaggtcg aaccggatct cccatccctg ccccaccgac gtcgacgatc
     5581 ccgcgttgta cacggccgtg gccttgtcga tcgcgtcccc ggtgatctcg accggaccga
     5641 gctggtacct ggtggcgtcg tcccggtcct ggaagacgac ctcctggtcc ttgagcgcct
     5701 cgaacgagca ctccgtggtc gcctgctcgg cgggggtgcc gcaggtgagc ggggtcgagg
     5761 cgtacgcggg gtccgtcggg gcgagcaccc cgatcacctc gcgctgttcg aggcgggccg
     5821 tctcgccgat cacctggagc aggcgctcct ggccggtctc ctggagccgg gcctcggcgg
     5881 cctcgcggtc gaggaagcac cccaggttct cccccgccga gctcacgacg cagaactgcg
     5941 tgtcagcgtt gcggatggtc atgccgtcca gcaacgtctg ggcttcctcc tgcgacgggg
     6001 tgcacgcggc ccccagatcg ctcgtgagcg tctccttgcc ctcggtctgg atgcagaagc
     6061 tctgggacgt caccgtctcg atggagctca acgcctggtc ggcctcgtcc cgggtcggat
     6121 agcagggatc cgtcgcgagc cccgttccgg tcaggcagaa ctcctcctgc tgcttcttga
     6181 cggcgatcgc gtcgagcaca gtggtcgcat ccttctcggt gccgacgcag ggagcgtcct
     6241 ctccgaacag cgtcggcgtc gactcgttgg cgggatcgtc ggtcaggcag acggacgaca
     6301 caaccggtcg aacgcttgcg gcgccgagcg ccccatcggc ggcgtcctgc gtgtcgaaac
     6361 acgtgtagac gttccccttg tcgtcggcga tgcacgactg ctgcttgggc cgctcctgga
     6421 tcgtcccgcg cgccagaccg gggatctgga cctcgatcgt gttgccggtg acgaacagga
     6481 gcggttcggc cgtcccgaac gcgtcgatgc ggttgcggat gttctccagc gcctggttca
     6541 tcacggtgtc cggcgtcccg tccggggcgg acaggatcac cgacacgccg ccctcgaggt
     6601 ccagaccgag gaccgggcgc gtcccggtga cgaacagggc gatcgcgccg acggcgagga
     6661 gccccaccac aaccagcgag acccagaacg atcgctgctt cttcctcatc gggatccttc
     6721 cgcctcgctc aagcgagctc ggcgccgttg atcaggagtt tgaacctgca attcagcacc
     6781 tcggccgcga ggcggcacag ctgcatgcgc ccgaggaaga tctcgaagta ctccatgacc
     6841 tggctgatct tcgtatcgat cgtcagctcg aggctcacgg tcttggccac ggagtctacc
     6901 cgcaggaacg actgctcggc ggcgaagttc acgcggtcgt ggatgtcgaa ctcgatctgg
     6961 ccggacttgc gcacccggct ccggtggacg tcagacttgt ccgccaggat caccgcggcc
     7021 gcgaccggag agacggcgta cccgttgtct tcctcgtggt tcgcgatcgc ggccacgatc
     7081 tgcgcgaggt ccgtggaggg cacccggtcg cgcagagcct ggtagaccag gacgcctccc
     7141 gtctgaccgt gcgcgtagcg gctgatcatg ttccccacgt cgtgaaggta accggccacg
     7201 caggccaggt tggcggtgtg cgtgtcgaac ccggctcgcg acagaacgtt gaacgcgatc
     7261 cgggccgtga ggttggcgtg gcggaacccg tgctcggtgt acccgagccc ctccatcacc
     7321 cggtcggccg ccgcgatgaa cgacgagagc tcctcgtcat gttgcacgac ctcgagggtg
     7381 atgccttcgg gctggctggc ctcctcggcc ggctccgacg gaccctcggc cggtgccggt
     7441 tcggcgctca cggctctcgg tcgatcccct cgtcggcgtt ctcgtcgccc ccgtcgtcct
     7501 cgtacgtgtc ctcgacgaag cgctgggaga tgccctggcg gagcatgcgg atccgggtgc
     7561 cgggcgcgat ctcgacggtc actgtgccct cgtcgtcgtc gatctcgacg atcgtgccga
     7621 agatcccacc ggtggtcatg acgtcgtcgc cgacctcgag cgaggcgacc agcgcctgct
     7681 gcgcgcgctg gcggttccgc tgcggccgga tgagcaggaa gtagaagccg acgatcatga
     7741 gcacgggcag cgcgagcccg atgaaggaac ttccgctttg ggagtcttga gcgaggatcc
     7801 cgagcaccat gggtcgacga gcctcctacg gggggtgacg aggcagcata caggctaccg
     7861 ctcggttcgc cgcccgcagg ggcggggttc acgccggtca actgagcggg aggacccctt
     7921 ccggcgagac cccaaggtgc cggtaggcac gcgcggtcgc caggcggccc ctcggcgtgc
     7981 gctggaggag gccgagctgg agcaggtagg gctcgacgac gtcctcgatc gtgtcggtct
     8041 cctcccccac cgccgccgac agcgtcgaga gcccgacagg ccctccgccg aacttgacga
     8101 ccacggtgga aaggatcgcg aggtcgagct tgtccaggcc ctcctcgtcc acctcgaaca
     8161 gctcgagccc ggccctcgcg acgtccgagg tgacgttgcc gtcgtggcgc acctcggcga
     8221 agtcgcggac ccgtcgcagc agccggttcg cgaccctcgg cgtgccgcgg gagcgcctgg
     8281 cgatctcgtg ggtcccctcg gcgtcgatcg acacgccgag gatccccgcc gagcgccgca
     8341 cgatcgcccc gaggtcgtcg atcgagtagt agtccagccg gggggagaac ccgaaccgct
     8401 cgcgcagcgg cagcgtgatc cgccccgggc gcgtcgtcgc gccgaccagc gtgaacctcg
     8461 gcaggtccag acggatgctc cgcgcgctcg ggcccttgcc gagcaccacg tcgagcttga
     8521 agtcctcgag cgccgggtac aggacctcct ccaccgcgcg cggcatccgg tggatctcgt
     8581 ccacgaacag gacgtcgccc tcctcgaggt tggtcaggat cgcggcgagg tcgccgggcc
     8641 ggtcgagcgc gggtccggac gtcggctgga accccacgcc tagctcgttc gagacgatgc
     8701 cggcgagcgt ggtcttgccg agcccggggg gcccggagaa caggaggtgg tcgacggtct
     8761 cgcctcgagc gcgggcgccg tcgaccagga gcgcgagctg ctccttgatg cggtcctggc
     8821 cgacgaactc gtcgatccgg cgcgggcgga gcgcagcgtc gaactcgcgc tccttctcgg
     8881 gcacctcggg gtcgacgatc cgctcgtcct gggtcacgtc ggccgtcacc ctacccgcgc
     8941 ccgatgcgtt gaagcgcctc acggagcaga tcctcgacgg acgagtcgcc atgggtcgta
     9001 agaccggtca tcgcctcgct cgcctcctgc ggtgacagtc cgagcgcgag cagcgcctcg
     9061 cggacgtcgg ccagcggacc gtcgatgatc accacgtcct ccgcgccgag cttgtccttc
     9121 aggtccagga ccacccgctg cgcgaccttc ttcccgacgc ccgggacgac ggtgagcgcg
     9181 gcgacatcgc ccgaggagac cgcgcgccg
//
LOCUS       DATBRK010000185         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1474243, whole genome shotgun sequence.
ACCESSION   DATBRK010000185 DATBRK010000000
VERSION     DATBRK010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474243"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VIH70_05565"
     CDS             complement(<1..294)
                     /locus_tag="VIH70_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8115864.1"
                     /translation="MQARYSRWDDTQDPFGPELSAGELLEELSDDALSGLGIEGALSR
                     LLRRGVRGSFDGLDALRARLRERRAREQANLDLAGPLEELRERLDDIVDRERST"
     gene            complement(335..1768)
                     /locus_tag="VIH70_05570"
     CDS             complement(335..1768)
                     /locus_tag="VIH70_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8115865.1"
                     /translation="MDEDVSAFASDFNRFLEAMSAAAATAQETPVRDLLDRHLGDRSE
                     VLPVVAEGYASYDHVNLQVALDAYVAANGRTADVIGLSGQQRRWMSLSDLVATAHHAG
                     VGIGSVDLVNLPVGPEDTLACIAFGLYLIDDHGTKLVALVRAADEQSGQAEVTLEILC
                     SDRNLARAMLDEIRELMVERNVFRGQMISFGESRMGHMTLGPIVFHRRLVLERHAIVL
                     PEGLLDRVERHVFGIAEHRERLRAGGQHVKRGLLLHGPPGTGKTLTVRYVAGRARDHT
                     IVVLAGGGLGMVRPACALARMLQPSIVVLEDVDLVAQHRGYVESNPLLFDILNQMDGI
                     DEDADVAFLLTTNRADLLEPALAARPGRVDLALHMPLPDDDGRLGLIRLYGGDLELRL
                     EDPDTVVRRTAGVTASFIKELIRKAALIASLEADGTGAPTVTDAHLGEALDELLSDES
                     EMTRALLGGAATSSRPGNEWLLQAGPE"
     gene            complement(1800..>2039)
                     /locus_tag="VIH70_05575"
     CDS             complement(1800..>2039)
                     /locus_tag="VIH70_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115866.1"
                     /translation="QTVAILQALIGVILLIAGKRPTTGLHYVYGFGPLVVFAIGHVLG
                     REEPFRARPWIPFAMASFISFGLSLRALTTGLGIG"
BASE COUNT          324 a          727 c          697 g          291 t
ORIGIN      
        1 cgtcgagcgc tcgcgatcga cgatgtcgtc gagccgctcg cggagctcct cgagcgggcc
       61 ggccaggtcg aggttcgcct gctctcgcgc gcgccgctcg cgcagccgag cacggagggc
      121 gtccagcccg tcgaagctcc cgcgcacccc gcggcgcagc agccgcgaga gcgcgccctc
      181 gatccccaga cccgacaacg cgtcgtcgga cagctcttcg agcagctcgc ccgcggacag
      241 ctcggggccg aacgggtcct gcgtgtcgtc ccagcgcgag tagcgagcct gcacccgacc
      301 agcctacgcc ggactcggtg gaacgacgcc tcggttactc gggtccggcc tgcaggagcc
      361 actcgttgcc gggtcgggac gacgtggcgg cgccaccgag gagcgcccgg gtcatctcgc
      421 tctcgtccga gagcagctcg tcgagcgcct cacccaggtg cgcgtcggtg accgtcgggg
      481 caccggtccc gtcggcctcg agcgaggcga tcagcgcggc cttgcgtatc agctccttga
      541 tgaacgacgc ggtgacaccg gcggtcctgc gaacgacggt gtcggggtcc tcgagccgca
      601 gctcgaggtc ccctccgtac agccggatca ggccgaggcg gccgtcgtcg tcgggcagcg
      661 gcatgtggag cgcgaggtcc acgcgaccgg gacgcgccgc gagcgccggc tcgagcaggt
      721 cggcgcggtt ggtcgtgagc aggaacgcga cgtccgcgtc ctcgtcgatc ccgtccatct
      781 ggttcaggat gtcgaacagc agtgggttcg actcgacgta cccgcggtgc tgcgcgacga
      841 gatccacgtc ctccaggacc acgatcgacg gctggagcat cctcgccagg gcacacgcgg
      901 gccggaccat cccgagtcca ccgccggcga gcacgacgat cgtgtggtcg cgggctcgac
      961 cggccacgta ccggacagtg agcgtcttgc ccgtgcccgg gggtccgtgc aggaggaggc
     1021 cccgcttcac atgctgcccg ccggcccgca ggcgctcacg atgctcggcg atcccgaaca
     1081 catggcgctc caccctgtcg agcagccctt ccgggagcac gatcgcgtga cgctcgagca
     1141 ccaggcgccg gtggaacacg atgggaccca gggtcatgtg gcccatccgc gattcgccga
     1201 aggagatcat ctgaccgcgg aacacgttcc gttccaccat cagctccctg atctcgtcga
     1261 gcatcgcacg ggccagattg cgatccgagc acaggatctc cagcgtcacc tccgcctgcc
     1321 ccgactgctc gtcggcggct cgcacgagcg cgacgagctt ggtgccgtgg tcgtcgatca
     1381 ggtacagacc gaacgcgatg caggcgagcg tgtcctcggg accgaccgga aggttcacga
     1441 ggtccaccga gccgatgccc acgcccgcgt gatgcgccgt tgcaaccagg tcggagagcg
     1501 acatccaccg gcgttgctga cccgacagac cgatcacgtc ggccgtccgg ccgttcgcgg
     1561 cgacgtaggc gtcgagtgcc acctggaggt tcacatggtc gtaggacgcg tacccttcgg
     1621 cgaccaccgg gagcacctcg ctccgatcgc cgagatgacg gtcgaggagg tcacgcaccg
     1681 gagtctcctg cgcggtcgct gccgcggcgg acatcgcttc gaggaagcgg ttgaagtcgc
     1741 tcgcgaaggc cgagacgtcc tcgtccatgt ctcgagggta gcccgttcgg cccccgaggt
     1801 cagccgatgc cgagtcccgt cgtgagcgct cgaagggaca ggccgaacga gatgaacgac
     1861 gccatggcga acgggatcca cggcctcgcg cggaacggct cttccctccc caggacgtgg
     1921 ccgatcgcga agaccaccag cggcccgaac ccgtagacgt agtgcagccc cgtggtgggt
     1981 cgctttcccg cgatcagcag gatcacgccg atcagcgcct gaaggatggc cacggtctg
//
LOCUS       DATBRK010000186         4417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_892868, whole genome shotgun sequence.
ACCESSION   DATBRK010000186 DATBRK010000000
VERSION     DATBRK010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892868"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1043
                     /locus_tag="VIH70_05580"
     CDS             90..1043
                     /locus_tag="VIH70_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8115867.1"
                     /translation="MTEAEGARALFKQVADRVDERLFGVREPLGLVMVGILTGNHVLI
                     DDVPGVGKTTLVRMLSTLLGLRFQRVQFTPDLMPSDITGTSVLNLRTNDFEFRPGPLF
                     TQVLLADEINRATPKTQAALLEAMQERQVTVDGETHPLPDPFFVLATQNPIELEGTFP
                     LPEAQLDRFLLRVKLGYPDEREELRILQTRPAERTLPREPLADRPPLRELRDAIASIG
                     LEQPVRAYVVALARATRDHPELSVGASPRAVEQMGDAARAFAMLDGRDYVLPDDVKTL
                     AEPVFGHRLVPTTDARIRDRNADEILREILRSVPVPAELDT"
     gene            1131..2204
                     /locus_tag="VIH70_05585"
     CDS             1131..2204
                     /locus_tag="VIH70_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8115868.1"
                     /translation="MVVLLWASAIAAISIASTLWARSALRGVSVEVAFEPSRAFVGED
                     VALRVRIANEKRLPLPIVRVLVRYPFGLLPGAAPDPTALRGHRRRLSLAGRSEVELRL
                     PVVVTTRGEYWLEGVDLVVSDPFELAPVKLEATTDRPLLVMPEPRVGIPLRIARRLPF
                     GSPAPSAQLFEDREHFAGVRDYEPGDPMHHVHWRLSAHAGKLQTKRYEPTRSAEVLFA
                     LDVSNGEPFWHAVDPDTAEESIGWTSYLARQAIRAGWRTGLVANTHLRRGRGPLRVRT
                     SDSAGNEAVLFAALARMPNQPTSDLGPVLREVGRRLVRRTTVIVVSPRPGPALSHEME
                     VLRRRGCDVIHLSPLEAFRKVAS"
     gene            2227..3528
                     /locus_tag="VIH70_05590"
     CDS             2227..3528
                     /locus_tag="VIH70_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY8115869.1"
                     /translation="MASAWVGTTRPEARELRACGLAALAEAGVLVLPVWLVLTESRGL
                     EIGVVALAVPFVAAYVGGAVLACRFRASRNVAFGAAMLAVVSGIWLGHGDLNRIVFSV
                     LVCLLVSFRLVTLALRDWRVPIHAEIGWLALALGLEVMVAAGATDEWRGPLLLFVPLF
                     FVAALASRASTVWTSGGAGELDEHVRAAWIRRALIATGALVGAMATAVILSVEGGVFD
                     RIGVWLTPAANAVASFFAWLLSQAARPIFFLVDLLGIDPDRVRELLDSLREGAAGRAI
                     DQRKAGPPEIWQRMLGFLVFAAIGYAVFRAIRHFRPPAGAEPEPRVPGTQTEAPLPAV
                     EALAPRPRFRREPPADTVRRWYAEALWALRRREVVREPWQTPAEFAPVVAGAVPSCAD
                     AFDELTRAYEDVRYGSLRIDATAIGRLEAGQQRIMSAIGSE"
     gene            3744..>4417
                     /locus_tag="VIH70_05595"
     CDS             3744..>4417
                     /locus_tag="VIH70_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8115870.1"
                     /translation="MTDPAIVVESLAKRFGKVEALKGVDLQVPPSTVLGMLGPNGAGK
                     TTTVRILTTLLSPDEGHATVAGYDVVKHAEELRHVIGLAGQNAAVDENLTGLENLEMV
                     GRLYHVPAAEAKRRGQEILERFELSDAAHRVAKTYSGGMRRRLDLGASLVGRPQVLFL
                     DEPTTGLDPRGRMEMWDVIRDLRTEGTTLLLTTQYLDEADRLADSIAVIDGGRVIAEG
                     TSDQLKA"
BASE COUNT          570 a         1591 c         1596 g          660 t
ORIGIN      
        1 cgcgcctcga cgctcgccga gacgggctcg gttcctcctg ggcaggaaac cgcaaccccg
       61 tccccctcac ctgggcctat ccttgctcga tgaccgaggc cgagggggcg cgggctctct
      121 tcaagcaggt cgccgaccgg gtcgacgagc ggctgttcgg cgtgcgcgag ccgctcgggc
      181 tggtgatggt cggcatcctc acgggcaacc acgtcctgat cgacgacgtc cccggggtcg
      241 gcaagaccac gcttgtccgg atgctctcga cgctgctcgg cctccggttc cagcgggtgc
      301 agttcacgcc cgacctgatg ccgagcgaca tcacaggcac ctcggtcctg aacctcagga
      361 ccaacgactt cgagttccgg ccgggcccgc tgttcacaca agtcctgctc gccgacgaga
      421 tcaatcgcgc cacccccaag acgcaggcgg ccctcctgga ggcgatgcag gagcgccagg
      481 tgaccgtcga cggcgagacc catccgctcc ccgacccgtt cttcgtcctc gcgacgcaga
      541 acccgatcga gctcgagggc acgttcccgc tgcccgaggc gcagctcgac cgcttcctac
      601 tccgggtcaa gctcgggtac cccgacgagc gcgaggagct ccggatcctg cagacccggc
      661 cggccgaacg caccctgccg cgggagccgc tcgccgaccg gccgccgctg cgggagctcc
      721 gtgacgcgat cgcgtcgatc ggcctggagc agccggtgcg ggcctacgtg gtcgccctcg
      781 cgcgcgcgac acgcgaccat ccggaactgt cggtcggcgc gagccctcgg gccgtcgagc
      841 agatgggcga cgccgcgcgc gcgttcgcga tgctcgacgg ccgcgactac gtcctgcccg
      901 acgacgtgaa gacgctcgcc gaacccgtct tcggccaccg gctcgtcccg acaaccgacg
      961 cgaggatccg cgaccggaac gccgacgaga tcctccgcga gatcctgcgc tcggtccccg
     1021 tgcccgccga gctcgacacc tagggagtcg gcacggacac ggcggaacgg cgccagatcg
     1081 cgctctacgt cgcgggcatc gtcgggctgt tcgcggtcgc atggggacag gtggtcgtct
     1141 tgctgtgggc gtcggcgatc gcggcgatct cgatcgcgtc gacgctgtgg gcgcgttcgg
     1201 cgctccgcgg cgtgagcgtc gaggtcgcgt tcgaaccgtc gagggcgttc gtgggagagg
     1261 acgtcgcgct gcgcgtccgg atcgccaacg agaagcgcct tcccctcccg atcgtccgcg
     1321 tgctggtccg ctatcccttc ggcctcctgc ccggtgcggc ccccgacccc accgcgctcc
     1381 gcggacaccg acgacgactc tcgctcgcgg ggcgaagcga ggtggagctc cgtctgcccg
     1441 tcgtggtcac cacgcgcggc gagtactggc tcgagggcgt cgacctcgtg gtctccgatc
     1501 cgttcgagct cgccccggtc aagctcgagg ccacgacgga ccggccgctg ctcgtgatgc
     1561 cggagccccg cgttgggatc ccgctccgga tcgcccggcg gctgcccttc ggttcgccgg
     1621 cgccgtccgc gcagctgttc gaggaccggg agcacttcgc gggtgttcgc gactacgagc
     1681 ccggcgaccc gatgcaccac gtgcactggc gcctctccgc ccacgcgggc aagcttcaga
     1741 cgaagcgcta cgagccgacg cgctcggccg aggtcctgtt cgcgctcgac gtctcgaacg
     1801 gcgagccgtt ctggcacgcc gtcgaccccg acaccgcgga ggagtcgatc ggctggacgt
     1861 cgtacctcgc ccgccaggcg atccgcgcgg gctggcgcac gggcctggtc gcgaacacgc
     1921 acctgcgacg cggccgcggg cccctgcgcg tgcggacatc cgactcggcc ggcaacgagg
     1981 cagtgttgtt cgcggccctc gcccgcatgc cgaaccaacc gaccagcgac ctggggcccg
     2041 tgctgcgcga ggtcggccgg cggctggtcc gccggaccac cgtcatcgtg gtctcgccgc
     2101 ggccgggacc ggcgctgtcc cacgagatgg aggtgctccg ccggcgcggg tgcgacgtga
     2161 tccacctctc gccgctcgag gcgttccgca aggtcgcgtc gtgacgcgcc caggaccggt
     2221 ctcgtgatgg cgtccgcctg ggtcggtacc acgcggcccg aggcccgcga gctccgcgcg
     2281 tgcgggctgg ccgcgctcgc cgaggccggc gtgctcgtcc tgccggtgtg gttggtcctg
     2341 acggaatcac gcgggttgga gatcggcgtg gtggcgctcg cggtgccgtt cgtcgcggcg
     2401 tacgtggggg gcgcggtgct cgcgtgccgg ttccgggcct cgcgcaacgt cgcgttcggg
     2461 gcggcgatgc tcgccgtggt gagcgggatc tggctcggac acggcgacct caaccggatc
     2521 gtgttctcgg tcctcgtgtg cctgctcgtg tcgttccggc tcgtcacgct ggcgctgcgg
     2581 gactggcgcg tgccgatcca cgcggagatc ggctggctcg cgctcgcgct cggactcgag
     2641 gtgatggttg ccgccggggc aaccgacgag tggcgcggcc cgctgctgct gttcgtgccg
     2701 ctgttcttcg tggccgcgct cgccagccgt gcgagcacgg tctggacctc gggcggcgcc
     2761 ggcgagctcg acgagcacgt ccgtgccgcg tggatccgtc gagcgctcat cgcgaccggc
     2821 gcgctggtgg gcgcgatggc caccgcggtg atcctgagcg tcgagggcgg cgtgttcgac
     2881 cgcatcggcg tctggctcac cccggcggcc aacgccgtcg cctcgttctt cgcgtggctg
     2941 ctctcgcagg ccgcgcggcc gatcttcttc ctcgtcgacc tcctcggtat cgaccccgac
     3001 cgtgttcgcg aattgctcga cagcctccgc gagggcgcgg cgggtcgcgc gatcgaccag
     3061 cggaaggcgg ggccgcccga gatctggcag cggatgctcg ggttcctcgt gttcgcggcg
     3121 atcgggtacg ccgtgttccg ggcgatccga catttccggc cgccggccgg cgccgagccc
     3181 gagcctcggg ttcccgggac ccagaccgag gcgccgctgc ccgctgtcga ggcgctcgcc
     3241 cccaggcccc ggttccggcg tgagccgccc gcggacacgg ttcggcgctg gtacgccgag
     3301 gcactgtggg cactccggcg gcgggaggtc gttcgcgagc cctggcagac gcccgccgaa
     3361 ttcgcccccg tggtggccgg cgcggtgccc tcgtgcgcgg acgcgttcga cgagctcacg
     3421 cgcgcgtacg aggacgttcg gtacggcagc ctgcggatcg acgcgaccgc gatcgggcgg
     3481 ctcgaggctg gccagcagcg gatcatgtct gcgatcggga gcgaatgagc ctcgcccccc
     3541 gcggacgtcg atccggggct cccgggtccg acccaggaag gttccggtgt ttcgatgagt
     3601 tcggggcccg aacgcggtct ccacgtcatg cgggagggtc actcctaacg acctggacca
     3661 gggaggagcg gcgtgacgca cggtttcgga tgcgcacgag ggcgaggggc ggggttgacg
     3721 tcgcgggtag gatggccggc tccatgaccg atccggcgat cgtcgtcgag agcctcgcca
     3781 agcggttcgg gaaggtcgag gcgctgaagg gcgtggacct gcaggtgccg cccagcacgg
     3841 tcctgggcat gctcggcccc aacggggcgg gcaagaccac taccgttcgg atcctcacca
     3901 cgctcctcag cccggacgag ggacacgcca ccgtcgccgg ctacgacgtg gtcaagcacg
     3961 cggaagagct gaggcacgtg atcgggctcg ccggccagaa cgccgcggtc gacgagaacc
     4021 tgacgggcct ggagaacctc gagatggtgg ggcgcctgta ccacgtgccg gcggccgagg
     4081 cgaagcgacg cggccaggag atcctcgagc gtttcgagct gtcggacgcc gcccaccgcg
     4141 tggccaagac gtactccggc ggcatgcgtc ggcgactgga cctgggggcc agtctcgtcg
     4201 ggcgcccgca ggtcctgttc ctggacgagc ccacgaccgg actggaccct cggggccgaa
     4261 tggagatgtg ggatgtcatc cgcgacctgc ggaccgaggg aaccaccctg ctgctcacga
     4321 cgcagtacct cgatgaggcc gaccgcctgg cggactcgat cgccgtgatc gacggcggcc
     4381 gggtgatcgc cgagggcacg tcggaccagc tgaaggc
//
LOCUS       DATBRK010000187        10630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_29979, whole genome shotgun sequence.
ACCESSION   DATBRK010000187 DATBRK010000000
VERSION     DATBRK010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29979"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VIH70_05600"
     CDS             complement(<1..462)
                     /locus_tag="VIH70_05600"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEY8115871.1"
                     /translation="MQLVEHLADSGIAPTSVATAIERGALSLSFLDNMPGPAPRLERT
                     HSDLCHELGIPFELLDRIYIGFGLPRPHPDELVRADDWEIISGLPILFAAGLGEAEIL
                     RVARVWGEGPRRVAEHQVRAFHERFEEPYRQQGLPDDQVLGRALSEVGVQVT"
     gene            866..2260
                     /locus_tag="VIH70_05605"
     CDS             866..2260
                     /locus_tag="VIH70_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8115872.1"
                     /translation="MSSSSISISKLRTDFGGRVIGPEDPEYDEARTVFYGGIDRRPAA
                     IVRVKDDMDVSRVVSLAREAGVELAVRSGGHSIPGHGVSDGGIVIDLSDLRGFDIDVE
                     GRTVWAQTGLTAGEFSAAANAYGLGVGFGDTGSVGIGGITLGGGIGYLARKHGLTIDD
                     LLAADVVTADGEIVRADAETEPDLFWAIRGGGGNFGVATRFRFRLHQLDTVVGGMLML
                     PATAETIAGFMAESEASPEELGTIANVMKAPPLPFVPEEAHGKLVIMALMAYAGDTDT
                     GRRALAPFRALAEPVADMLRPMPYPEVYPPEQEGYHPVAITRTMFVDHIGRAEAETIL
                     QHLETSTATAGRGPVGGGVAQLRTLGGAMARVPIDATAFAHRQSRIMVNVVAIFERPD
                     EREVHGPWVDAFAGALHQGDDGAYVNFLGDEGEERVRAAYPGATWDRLRQIKARYDPT
                     NLFRLNQNIPPAAA"
     gene            complement(2395..2955)
                     /locus_tag="VIH70_05610"
     CDS             complement(2395..2955)
                     /locus_tag="VIH70_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8115873.1"
                     /translation="MGKVIISTHITVDGVIGPSPQDWAILEGEGERYKFDQLLAADAF
                     LLGRKVYEGLAGVWPTITDDTGFADRVNSIPKYIASRTLEEPLEWNASLIKGDLAESV
                     TSLKAQHRGNLISFGCGELAYELVTRGLADEIHFWVGPAVWGSGDRAFHGRQVRMRLI
                     ATTAFDSGVVLLSYRPVPNAQAGAAT"
     gene            3046..6552
                     /locus_tag="VIH70_05615"
     CDS             3046..6552
                     /locus_tag="VIH70_05615"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8115874.1"
                     /translation="MPACPVCDADNPIGARFCNACGARLTPPEAPTPEPAGERRVVTM
                     LFCDVRGSTSMAERLDPEEWTDIMNGAYEHLITPVYRYEGTVARLMGDAILAFFGAPT
                     AHEDDPQRAVMAGLEIVGSIAPFRDRLARERGLDLNVRVGINTGPVVVGEVGSDLRRE
                     YTAMGDAVNVAARMEQAAEPGTVQITEDTYRLVHDLFDVEPLGGVELKGKSRPVSSYR
                     VRGRLASPWKVRAARPLEAALIGRDREIGVIGSALRGVERQGGSVLMITGDPGLGKSR
                     LVEEAKAIWADLVPDDDHRWDTWQCVPYDTMQPYAQYRRVIRERADIAETDGAETVRA
                     KIAELMKTAPEGWADRSERVARALLGVEREDEPRLEGERFQREVTDLLVGSALAQGGR
                     RLIVFEDLHWCDHASLELIRATVRLVGEAPIVFLITFRPDRAAASWRFKEWVETELPG
                     RSNVIELAPLSREQSDRLIGELLPLGDAPGAITSRIVAATEGNPLFVQEVARAMIDRG
                     VVEPIHGGWRLAGASFEVAIPDSVQSLIMAGLDRLPERARGTLQAASVIGRSFDGGLL
                     RAVVDGEAVEDDLEELVRRDLIWRTAGGSRSEYTFRHALTAEAAYGSLLVRRRRELHR
                     RVAEAIRAASGDHLDDAASLLAHHFRESGDDEQTLLYAERAGAAAARLYAHVEAGGHY
                     RNALTAARRLGVESPRLRSLYERRGTALELAGRYDDAIDNYEEMRTEARDRGDESFEL
                     AAATRIALLYSTPTPKFDPEQGRRLSGENVAIARRLGDRGAEARALWNIVVANVYGGG
                     DAATAVEAGEASLAIARELGDREQLAFTLNDVARAHMAHADFATAAERLDEARGLWED
                     LGNGPMLGENLTVTGNIHALHGDDEAALSVARRALTISEEIDNPWGQSIALMSIYRAE
                     IVRGELGAALRSLERCRELGERGGFSFAGIGTRADLAKIHAYVGDGAGALGLANESLE
                     IADEQLPPAASVAHAARAEALILLGDAPGAQEAVDAVDPTKLPEPDRKFILVSSERAR
                     SRLALDAGDPAKAEAIARDLVDGLRSAGVRLMVAEGLVALARALTAAGRVDDAERALA
                     EAAESAERTGERGVLWEALALSSSLHAARGDHEAALDLRRRSATIVEGIAAGLDEDLR
                     ARFLSQDDVRAILEPRQQERTR"
     gene            6549..7421
                     /locus_tag="VIH70_05620"
     CDS             6549..7421
                     /locus_tag="VIH70_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8115875.1"
                     /translation="MTDPETHTLQVPGAVLRYDVRDAEGESGARVLVMIGFPMDASGF
                     STLVEYFRDRTVVTYDPRGVSRSVRTDGVTLSTPDERAGDLSRLISALDAGRVDIFAS
                     SGGAVDGLALVARHPEQVHTLVAHEPPAAQVLPDREQALAAARGIHETYVRRGSGPAM
                     ARFLELTSLKGPIPTDFADRPDPDPADFGLPMEDDGTRDDPMLSNIIATSHYEHDFVA
                     LRAAETRIVIGAGAESEGELAHRAALSVARLLGTEAVIFPSHHGGFLGGEFGWRGDPD
                     AFAATLRRVLAEND"
     gene            complement(7463..8359)
                     /locus_tag="VIH70_05625"
     CDS             complement(7463..8359)
                     /locus_tag="VIH70_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY8115876.1"
                     /translation="MRTIGLLGVPTNSAGTIDGVARGPLVLREAGLVDALLRHAKVHD
                     YGDVTLPDPSPTRESRSHVIDPDGLDALVAGVREAVASILDNGHLPLVVGGDCPLLLG
                     CLAASRAADRGGLLFVDGHEDAYLPMQSSTGEAADMELAFALGMADASWSPELAALLP
                     LVAPRDVRVLGARDAESLRAEGVASLRDRVALVDGDRLAADPAGSAVAACASLPDPWW
                     FHLDLDVLSTRALPAIDYPQPGGLGWDELAVVATTALSAGPRGWDVTIYNPDLDPTRV
                     HARRIVRFIGSVIEQVSDLEAF"
     gene            complement(8482..10530)
                     /locus_tag="VIH70_05630"
     CDS             complement(8482..10530)
                     /locus_tag="VIH70_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115877.1"
                     /translation="MRRGVLLGALSFLALSSSLVVAPIAHAAFPGQNGRIAYVTTSGD
                     HRVIETVDARGGDRQPLIDFGSGRDAIDPAWSWDGLKIAFAGQESPGGPFVIYTANAD
                     GTGSPQQLTTEGWPGWVSDTDPTWIPTGRVIAFVREFDDHSSEIWLVDVVTGSAGGSS
                     LHGLDAREPAWSPDAGRIAFTAREHTCVHAPCRWGIVIWDVSRAEYTSLYDPYFGDYD
                     WHHPDWAPDGTMIVARFGEDEAPLHVSGVRVFDVGSGHALDGPLTPCHIMTEPSFSPD
                     GEWVLVTATPIADPAGGDLGEPNLCATRTDNSETYLLEGAAPRGDATWGAVPGSEPPP
                     PSEFPPPPPPDTSPPTIEFRPEPSPTEWMATSWGGSVYVFATDDRPWPSIECTNNGSW
                     LPLTAWTTWRVGSSVKGVATLPEGRNDLECTARDSAGNSVSASGTYLVDLPPTIESVT
                     VSPAVARVGDEVSVSAVVSDAGSGVQSVDFAVNGATSGAVASGEMQASGSTFTASFVP
                     ASPDIFAIDVSARDGSGLITRSGVPFVAYDASAGTIDGTGWVVPGGPTSNSNDNLPGL
                     DGTTKASFSFTAKYKTSSSVTPAGGLTFSYGNRFKLQSKQLQWLAVADADTAYLGGVA
                     SIQGKYGDFPFVAVIVDGADTGGRDRFELWVYEPGATIDSQTLFSASGDAGGQVNIRT
                     "
BASE COUNT         1685 a         3643 c         3778 g         1524 t
ORIGIN      
        1 ggtgacctgg acgcccacct ccgacagcgc ccggccgagc acctgatcgt ccgggagccc
       61 ctgctgccgg tagggctcct cgaagcgctc gtggaacgcc cggacctggt gctcggcgac
      121 ccgccgcggc ccctcccccc acacgcgggc aacacgaagg atctcggcct ccccgaggcc
      181 ggcggcgaac aggatcggga ggccggagat gatctcccag tcatcggctc ggaccagctc
      241 gtccgggtgt ggtcgcggca gcccgaaccc gatgtagatc cggtcgagta gctcgaacgg
      301 gatgccgagc tcatggcaga gatcggagtg agtccgctcg aggcgaggcg cgggacccgg
      361 catgttgtcg aggaacgaca gcgacagggc gcctcgctcg atcgcggtgg cgacgctggt
      421 gggcgcgatg ccggaatcgg cgagatgctc gacgagctgc acccgcaggg catcgccctc
      481 gctgaacggt tggcgcgcgt tcggccccgg ccggacgatg ccgatggcga tcagctctcg
      541 gagacgctcg atggtcgtcc cagcgcgtgt cgcgagatct tcctcggtca gccccctggt
      601 cctctcatga ccttcccgac ccctgtgccg gacgcgatcc gaaggctccc gtgcacccac
      661 tccagcttca ggctacccat gctcgcctcc ggtcggacac tcgcggcatc tcagtctgag
      721 tcgaggaacg gtgtgtcaca gcgcgaaggg cttcctcgtc tcagtgttcg agacgaggca
      781 tgcccacgcc cgtggggggc atcgtgaccg atgagtttcc gcgcgacctc gggtcgttgc
      841 agataccaca tgggaggagg cggtcgtgag cagctccagc atttccatct cgaagctccg
      901 gaccgacttc ggcggaaggg tcatcggacc cgaggatccc gagtacgacg aggcgcgaac
      961 ggtcttctac gggggcatcg accgtcggcc ggcggccatc gtccgtgtca aggacgacat
     1021 ggacgtctcg cgggtcgtct cgttggcgcg cgaggccggt gtcgagctcg ccgttcggag
     1081 cggcggccac agcatccccg gccacggcgt atccgacggc gggatcgtga tcgacctgtc
     1141 cgacctgcgt gggttcgaca tcgacgtcga ggggcgcacc gtgtgggccc agacggggct
     1201 gaccgccggc gagttcagcg ccgcggccaa cgcgtacggc ctgggggtcg ggttcggcga
     1261 cacgggctcg gtcgggatcg gcgggataac gctcggcggc gggatcgggt acctcgcgcg
     1321 caagcacggc ctcacgatcg acgacctgct ggccgccgac gtcgtgacgg ccgacggcga
     1381 gatcgttcgc gccgatgccg agaccgaacc ggacctgttc tgggcgatcc ggggtggcgg
     1441 cggcaacttc ggcgtcgcga cccggttcag gttccgcctg caccagctcg acacggtcgt
     1501 cggcgggatg ctcatgcttc ccgcgacggc cgagaccatc gcagggttca tggccgagtc
     1561 ggaggcgtcg cccgaggagc tcgggacgat cgcgaacgtg atgaaggctc cgccgttgcc
     1621 gttcgtgccc gaggaggcgc acggcaagct cgtgatcatg gcgctgatgg cctacgccgg
     1681 agacacggac accgggcgac gcgccctcgc gccgttccgg gcgctcgccg agccggtggc
     1741 ggacatgctc cggcccatgc cgtaccccga ggtctacccg cccgaacagg agggctacca
     1801 ccccgtcgcg atcacccgga cgatgttcgt cgaccacatc ggccgcgccg aggccgagac
     1861 catcctccag cacctcgaga cctcgaccgc gacggcgggt cgcgggccgg tcggcggcgg
     1921 cgtggcacag ctccgaacgc tgggcggggc gatggcacgg gtgccgatcg atgcgacggc
     1981 gttcgcgcac cggcagagcc ggatcatggt caacgtcgtc gcgatcttcg agcgacccga
     2041 cgagcgcgag gtgcacggac catgggtcga tgcgttcgcc ggcgcactcc atcagggcga
     2101 cgacggggca tacgtcaact tcctcggcga cgagggggag gagcgggtcc gcgcggcgta
     2161 cccgggcgcc acgtgggatc gcctgaggca gatcaaggca cgctacgacc cgacgaacct
     2221 gttccgtctc aaccagaaca tcccaccggc cgccgcatga acgatcgcgc gcccggctag
     2281 gtcgggcccg cggaagcccc cggggacccc tcgagacggc gcttcgtcgc ggcgacatcg
     2341 gggtgcgtcc acgcggttgc atggggtggt ggccgaccgc cgagtctcgc gccgtcaggt
     2401 tgcggcgccg gcctgcgcgt tgggtaccgg ccggtaggag agaaggacga cgcccgagtc
     2461 gaaggcggtc gtagcgatca ggcgcatccg gacctggcgg ccgtggaagg cccggtcacc
     2521 gctgccccag accgcggggc cgacccagaa gtggatctcg tcggcgagcc cccgtgtgac
     2581 gagctcgtag gcgagctcgc cgcagccgaa ggagatcagg ttgccgcggt gctgcgcctt
     2641 caggctggtc acgctctcgg cgagatcgcc cttgatcaat gacgcgttcc actcgagagg
     2701 ctcttccagg gtccgggagg cgatgtactt ggggatgctg ttcacccggt cggcgaaacc
     2761 cgtgtcgtcg gtgatggtgg gccagacgcc ggccaggccc tcgtagacct tgcgcccgag
     2821 caggaacgcg tccgcggcca ggagctgatc gaacttgtac cgttcgccct cgccctccag
     2881 gatcgcccag tcctggggtg agggtccgat gacgccatcg acggtgatgt gggtcgagat
     2941 gatcaccttt cccacgggga cctccacgtt cgggcccggc cgcccagttc acgatagcct
     3001 cttcctgacg ctgtctcggc cgaggccggg agctatggtt cgaccatgcc ggcctgcccc
     3061 gtgtgcgatg cggacaatcc catcggggcg aggttctgca acgcctgcgg cgcgcgcctg
     3121 acgccgcccg aggcccccac gccggaaccc gccggcgagc gacgcgtcgt cacgatgctg
     3181 ttctgcgacg tgcgcggttc gacctcgatg gcggagcgcc tcgatccgga ggagtggacc
     3241 gacatcatga acggcgccta cgagcacctc atcacacccg tgtatcgcta cgagggcacc
     3301 gtggcacggc tcatgggcga cgcgatcctg gcgttcttcg gcgcaccgac ggcacacgag
     3361 gacgaccccc agcgtgcggt catggccggg ctcgagatcg tgggttcgat cgcgccgttc
     3421 cgagatcggc tcgcccgtga gcgcggcctc gacctgaacg ttcgtgtcgg catcaacacc
     3481 ggacccgtcg tcgtcggcga ggtgggaagc gacctcaggc gcgagtacac ggccatgggt
     3541 gacgccgtga acgtcgccgc acgcatggag caggcggccg agcccggaac ggtgcagatc
     3601 accgaggaca cctaccgcct cgtccacgac ctgttcgacg tggagccgct cggcggcgtc
     3661 gagttgaagg ggaagagccg gccggtgtcc tcgtatcgcg tgcgtgggcg gttggcttct
     3721 ccgtggaagg ttcgcgcggc cagaccgctg gaagcggcgt tgatcggtcg ggaccgggag
     3781 atcggcgtca tcggatccgc gctccgagga gtcgagcggc agggaggctc ggtcctcatg
     3841 atcacgggcg atccgggcct ggggaagagc cggctcgtcg aagaggcgaa ggcgatctgg
     3901 gcggatctgg tccccgacga cgaccatcga tgggacacct ggcagtgcgt gccctacgac
     3961 accatgcagc cctacgcgca gtaccgacgg gtgatccgcg agcgagccga catcgccgag
     4021 acggacgggg ccgagaccgt gcgggcaaag atcgccgagc tgatgaagac cgcgccggag
     4081 gggtgggccg acaggagcga acgcgtcgcc cgggcgctcc tcggcgtgga acgcgaggac
     4141 gagccgcgcc tcgaggggga gcggttccag cgagaggtga ccgacctcct ggtgggctcc
     4201 gccctcgccc agggcgggcg acggctgatc gtgttcgagg acctgcactg gtgcgaccac
     4261 gcgtcgttgg agctcatccg ggcgaccgtc cggctggtcg gcgaggcgcc gatcgtcttc
     4321 ctgatcacgt ttcggcccga ccgggccgcc gcctcgtggc ggttcaaaga gtgggtcgag
     4381 accgagctcc cgggtcggtc gaacgtcatc gagctcgctc ccctgtcgcg ggaacagagc
     4441 gatcgactga tcggcgagct gctcccgctc ggggacgcac ccggcgcgat cacgtcgcgg
     4501 atcgtcgccg ccaccgaggg caaccccctc ttcgtccagg aggtcgcccg ggcgatgatc
     4561 gatcgcggtg tcgtggagcc gatccatggc gggtggcggc tcgccggggc ctccttcgag
     4621 gtcgccatcc cggacagcgt gcagagcctc atcatggccg gcctcgaccg cctgccggag
     4681 cgcgcacgcg ggacgctcca agcggcgtcg gtcatcggca ggtcattcga cggcgggctg
     4741 cttcgcgccg tcgtcgacgg ggaagccgtc gaggacgacc tcgaggagct cgtccgacgc
     4801 gacctgatct ggcgcacggc cggcggctcc cgatccgagt acacgttccg gcatgcgctg
     4861 acggcggagg ccgcgtacgg gtccctgctg gtgaggcgtc gtcgggagtt gcatcgccgc
     4921 gtcgccgagg cgatccgagc ggcgagcggc gaccacctgg acgacgccgc gtcgctgctc
     4981 gcgcaccact tccgcgagag cggtgacgac gagcagacgc tgctctacgc cgagagggcc
     5041 ggcgccgcgg cggcgcgcct ctacgcccac gtggaggcgg ggggtcacta ccggaacgcg
     5101 ctgaccgccg cgcggcgcct cggcgtcgaa tccccacgcc tccgatcgct gtacgaacga
     5161 aggggcaccg ccctcgagct cgcggggcga tacgacgacg ccatcgacaa ctacgaagag
     5221 atgcgaaccg aggcgcgtga ccggggcgac gagtcgttcg agctcgccgc cgccacgagg
     5281 atcgcgctcc tgtactcgac cccgacgccg aagttcgatc ccgaacaagg gcgccggctg
     5341 tccggcgaga acgtggcgat cgcgcgccgt ctcggcgatc gtggggccga ggcacgcgcg
     5401 ctctggaaca tcgtggtggc caacgtctac gggggtggag acgccgcaac tgccgtcgag
     5461 gccggggagg cgtcgctcgc gatcgcacgg gagctgggcg accgcgagca actcgcgttc
     5521 acgttgaacg acgttgcgcg agcccacatg gcccatgccg acttcgcgac ggcggccgag
     5581 cgcctcgacg aggcccgggg cctgtgggaa gacctcggca acggcccgat gctgggagag
     5641 aacctgaccg tgaccggcaa catccacgcc ctgcacggag acgacgaggc ggcgctctcg
     5701 gtcgctcggc gagcgctcac gatctccgag gagatcgaca acccctgggg ccaatcgatc
     5761 gcgctgatgt cgatctatcg cgccgagatc gtgcgcgggg agctcggcgc ggccctccga
     5821 tccctggagc gctgccgcga gctcggggaa cgcggcgggt tctcgttcgc ggggatcggg
     5881 acgcgcgccg atctcgccaa gatccatgcc tacgtgggcg acggcgccgg cgcgcttgga
     5941 ttggcgaacg agtcgctcga gatcgccgac gagcagctcc ctcccgccgc gtcggtcgcg
     6001 cacgccgccc gagccgaggc gctgatcctc ctgggggacg ccccgggagc gcaggaggcg
     6061 gtcgatgcgg tcgacccgac gaagctgccg gagcccgatc gaaagttcat cctggtctcc
     6121 tccgagcgag cccgctcccg gctcgcgctc gatgccggcg atccggccaa ggccgaggcg
     6181 atcgcccgcg acctcgtgga cggtcttcga tcggccggcg tgcggctcat ggtggccgag
     6241 gggctcgtgg cgctcgcgag ggcgctcacc gccgccggcc gcgtcgacga cgccgagcga
     6301 gcgctcgccg aggcggccga gtcggccgaa cggaccggcg agcgaggggt gctctgggag
     6361 gcgctcgccc tgtcgtcctc cctgcacgca gcgcgaggcg accacgaggc cgcgctcgac
     6421 ctccgccgtc gttccgcgac gatcgtcgaa gggatcgccg cggggctgga cgaggacctc
     6481 cgagctcgat tcctctcgca ggacgacgtc cgggcgatcc tcgagccccg gcaacaagag
     6541 agaacccgat gaccgacccg gagacccaca ccctgcaggt gcccggcgcc gtcctgcggt
     6601 acgacgtacg agacgccgaa ggcgagagcg gagcgcgggt cctggtgatg atcgggtttc
     6661 cgatggacgc gagtgggttc tccacgctgg tcgagtactt ccgggaccga acggtggtga
     6721 cctacgaccc gcgcggggtc tcgcgcagcg tgaggaccga tggcgtcacc ctgtcaacgc
     6781 ccgacgagcg cgcgggcgac ctctcccggt tgatctccgc gctcgacgcg ggtcgcgtgg
     6841 acatcttcgc cagcagcggt ggagcggtcg acggcctcgc gctcgtggcc cggcacccgg
     6901 agcaggtcca tacgctcgtg gcgcacgaac cacccgccgc ccaggtgctg cccgaccgcg
     6961 agcaggcgct cgccgccgcc cggggtatcc acgagaccta cgtgcgccgg ggcagcgggc
     7021 cggcgatggc gagattcctc gagctcacga gcctgaaggg accgatcccg accgacttcg
     7081 ccgaccggcc cgaccccgat cccgccgact tcgggctccc gatggaggac gacggtaccc
     7141 gcgacgatcc catgctgagc aacatcatcg cgacctccca ctacgaacac gacttcgtcg
     7201 cactccgcgc ggccgagacc aggatcgtga tcggggccgg agccgaatcc gagggcgaac
     7261 tggcgcaccg cgcggcgctg tccgtggcga ggttgctcgg gacggaggcc gtcatcttcc
     7321 cgagccatca cggtggtttc ctcggcgggg agttcggttg gaggggagac ccggatgcgt
     7381 tcgccgcaac cctccgccgg gtgctcgccg agaacgactg acgcagacgt gggccggctc
     7441 aggatcccac cagctgcgtg actcagaagg cctcgagatc agacacctgc tcgatcacgg
     7501 atcctatgaa ccggacgatc cgtcgcgcgt ggacccgtgt gggatcgagg tcggggttgt
     7561 agatggtgac gtcccagcca cgagggccgg cgctcagggc cgtggtcgcg accaccgcga
     7621 gctcgtccca gccgaggccc ccgggctgcg ggtagtcgat cgccggaagc gcccgtgtcg
     7681 acaggacatc gaggtcgaga tggaaccacc acggatcggg aagcgacgcg caggccgcca
     7741 ccgcggatcc ggcaggatcg gccgcgagac gatcaccgtc gacgagcgcc acgcggtcgc
     7801 gcagcgaggc gacgccctcg gcgcggagcg actccgcgtc acgtgcaccg aggacacgga
     7861 cgtcgcgagg cgcgacgaga ggtagcaggg cggcgagctc cggggaccac gaggcgtccg
     7921 ccatccccag cgcgaacgcg agctccatgt cggccgcctc gcccgtcgac gactgcatgg
     7981 ggaggtaggc atcctcgtgc ccgtccacga acagaagacc acctcggtcc gctgcccggg
     8041 aggcggcgag gcaaccgagc agcagggggc aatcgccacc gacgaccagc gggaggtgcc
     8101 cgttgtcgag gatcgacgcg accgcctctc gcacgccggc gacgagcgca tcgaggccgt
     8161 ccggatcgat gacgtgggat cgcgattcac gcgtcggcga cgggtcgggc aacgtgacgt
     8221 ccccgtagtc gtgtaccttc gcgtgccgaa ggagggcatc gaccagaccc gcttcccgca
     8281 acacgagtgg accccgtgcg actccgtcga tcgtgcccgc cgagttcgtc ggcacaccaa
     8341 ggagcccgat cgtgcgcatc ccgatcgaaa ctacaccggc ggccagcgtt cggcggaggg
     8401 gcctccaccg gccatccggt cacctcgcgg gttcgagctg gtcgtgagct cgacggcggg
     8461 cggcgatgtc aacgggcccg gtcaggtgcg aatgttgacc tgtccccccg catcgcctga
     8521 cgccgagaag agcgtctgcg agtcgatggt ggcgcccggc tcgtaaaccc acagctcgaa
     8581 gcgatcgcga ccgcccgtgt ccgcgccgtc gacgatcacc gccacgaatg ggaagtcgcc
     8641 gtacttgccc tggatcgagg caacgccacc caagtaggcg gtgtccgcgt ccgccacggc
     8701 gagccactgg agctgcttgc tctgcagctt gaaccggttg ccgtatgaga acgtgagccc
     8761 gcccgccggg gtcaccgacg aggacgtctt gtacttcgcg gtgaatgaga acgacgcctt
     8821 cgtcgtcccg tcgagtccgg gcaggttgtc gttcgagttc gacgtcggac cgccgggcac
     8881 gacccagccg gtgccgtcga tcgtcccggc agacgcgtcg taggcgacga acggaacgcc
     8941 cgatctggtg atcaacccgg acccgtcgcg agccgacacg tcaatcgcga agatgtccgg
     9001 cgacgccggg acgaacgatg ccgtgaacgt cgatccggat gcctgcatct ctccgctcgc
     9061 gacggcgcct gacgtggcgc cgttgactgc gaaatccacc gactgcacgc cggagcccgc
     9121 gtcgctcacg accgccgaga ccgagacctc gtcgccgacc cgggccaccg caggcgaaac
     9181 cgtgacgctc tcgatcgtcg gcggcagatc gaccaggtac gtccccgatg ccgaaaccga
     9241 gttgcccgca gagtcccttg ccgtgcattc gaggtcgttc cgtccctcgg ggagtgttgc
     9301 gacccccttc acagacgatc ccacccgcca cgtggtccag gcggtcaagg gcagccacga
     9361 gccgttgttg gtgcactcga tggacggcca cggtcggtca tcggtggcaa agacgtagac
     9421 ggacccgccc cagctggtag ccatccactc ggtcggcgaa ggctctggcc ggaactcgat
     9481 ggtcggcggg gaggtgtccg gcggtggagg gggtggaaac tccgacgggg gtggtggctc
     9541 cgagcctgga accgcacccc atgtcgcgtc gcctcgtgga gctgctcctt cgagcaggta
     9601 cgtctcgctg ttgtcggtcc gggtggcgca gaggttcggc tcacccaagt cgccgccggc
     9661 ggggtcggct atgggcgtcg ccgtgacgag cacccattcg ccgtctgggg agaacgacgg
     9721 ctccgtcatg atgtggcatg gcgtcagcgg gccgtcgagc gcatgcccgg agccgacgtc
     9781 gaaaactcgg acaccggaga cgtgaagcgg ggcctcgtcc tccccgaatc gggccacgat
     9841 catggtgccg tcgggcgccc agtcgggatg gtgccagtcg taatcgccga agtatgggtc
     9901 gtagagagac gtgtactcgg ctcgagagac gtcccagatg acgatgcccc aacgacatgg
     9961 cgcgtggacg cacgtatgct ctctcgcggt gaacgcgatc cgcccggcgt cgggcgacca
    10021 agcaggctcc ctcgcgtcga gaccatgcag ggagctgccc ccggcggatc cggtcacaac
    10081 atcgaccaac cagatttccg acgagtggtc gtcgaattcc cggacgaacg cgatgacccg
    10141 gccggtgggt atccacgtcg gatccgtgtc cgacacccaa cccggccatc cttccgtcgt
    10201 gagttgttgc ggcgagcccg ttccatctgc gttcgccgtg tagatgacga acggccctcc
    10261 tggcgattcc tgccccgcga aggcgatctt gaggccatcc cacgaccacg cgggatcgat
    10321 ggcgtcgcga ccgctgccga agtcgatcaa cggttggcga tctccacccc gagcgtcgac
    10381 ggtctcgatg accctgtggt cgcccgaggt cgtcacgtag gcgatccggc cgttctgtcc
    10441 ggggaacgcg gcgtgcgcga tcggcgcgac cacgaggctc gacgagagcg ccaggaatga
    10501 gagtgcgccg agcaacacac ctcgtctcac gtgggaaagc ctccctgggt accgggctct
    10561 cgagggtaga agagggatgc cagccaatca atcggtcgaa tggcgaccgg gcgtcgtacg
    10621 gccttcggcc
//
LOCUS       DATBRK010000188        10647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1904586, whole genome shotgun sequence.
ACCESSION   DATBRK010000188 DATBRK010000000
VERSION     DATBRK010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904586"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="VIH70_05635"
     CDS             complement(<1..150)
                     /locus_tag="VIH70_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031386 - protein tag activity [Evidence
                     IEA];
                     GO_function: GO:0070628 - proteasome binding [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEY8115878.1"
                     /translation="MPQEQEQKKTQKRSGGGSDDEAGQVTSASKGEKLKEEMDDILDE
                     IDSVLE"
     gene            complement(394..1878)
                     /gene="dop"
                     /locus_tag="VIH70_05640"
     CDS             complement(394..1878)
                     /gene="dop"
                     /locus_tag="VIH70_05640"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEY8115879.1"
                     /translation="MAIPKVIGTETEYGISAAASPEFNPVISSSLLISTYAGALRRIR
                     WDYEQESPLRDARGFEPVQLREPSEEDLGLANVILPNGARYYVDHAHPEYSTPECVTP
                     RELVIHDKAGERVLERSLDEVRRTMPGAQRLAVYKNNSDGKGNSYGTHENYLVDRQVP
                     FANLVAGLTPFFVSRQIFCGAGKLGGEASWDERSRDRYQVTQRADFFEAEVGLETTLK
                     RPIINTRDEPHADPEKYRRLHVIVGDANLCEVATLLKLGTTSLVLKLIEDGDVPDLAV
                     ATPVPSLHDVSRDLTCTAPVRLADGRRLTAVQMQWEYLERARKYVEQEDTTEDNLEVL
                     ERWEAVLTGLEDDPMQLSRELDWVAKYRLLDGYRERGGLDWSDAKLRLIDLQYHDVRR
                     DKGLYHRLVATGQVERLVADEDVERAIMEPPDDTRAYFRGRCISRYPDAIAAASWDSI
                     IFDTGAEALQRVPMREPLRGTREHVDRLFEEAADAAALVEMLRS"
     gene            complement(1888..3639)
                     /gene="arc"
                     /locus_tag="VIH70_05645"
     CDS             complement(1888..3639)
                     /gene="arc"
                     /locus_tag="VIH70_05645"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017604893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEY8115880.1"
                     /translation="MPEPDPREQREQFEKQVHELQTQVRFLEDEVSLLRRRLTNAPRQ
                     VKLLEDKLLEARSDLSRALSQNEKLAGTLQAEKERIEGLREEVEKLSQPPASFGVYLG
                     VNDDGSVDVFTAGRKMRVNVSPDVETDRLRKGAEVILNEALNVVEVLLPDRAGEVVKV
                     KDRLGEDRVIVVGRGDEELVATLGTALLDEQIRAGDPLLIDPRSNVVLEKLPKEEVEE
                     LVLEEVPDVSYEDIGGLEGQIEAIRDAVELPFLYAELFQEHQLEAPKGVLLYGPPGCG
                     KTLIAKAVAKSLADKVRERTGRQDAHSYFLNIKGPELLNKYVGETERQIRQIFQRAKE
                     RSEEGLPVIVFFDEMDSIFRTRGTGISSDVESTIVPQLLSELDGVETLKNVIVIGASN
                     REDLIDPAILRPGRLDVKIKIERPDAAQAADIMAKYLNPNVPIHPEDVARASGDVKVT
                     TTRMIQSTVDRMYSEAEDNRFLEVTYASGDKEILYFKDFNSGAMIENIVRRAKKEAIK
                     RFLSTGEKGIKAEDLSHAIRDEFKENEDLPNTTNPDDWARISGKKGERIVYVRTLLGD
                     GDGEGRQVERVSAGQYL"
     gene            complement(3791..4567)
                     /locus_tag="VIH70_05650"
     CDS             complement(3791..4567)
                     /locus_tag="VIH70_05650"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEY8115881.1"
                     /translation="MNGPFEAGEKILLVDQRDRRYLLTLQTGETWHSHGGGLPHDLLI
                     GSPEGTLVHSATGMAFRAFRPRLADFVLKMPRDAQLVYPKDIGAILIGADVFPGARVL
                     EAGTGSGSLTLALCRATGPEGRVVSYDLRREFQTGAARNIESFFGKAPDWLELREGDV
                     RDVGGSGETFDRAVLDLPEPWQTLEPLSTVLPPGAIFCGYLPTTNQVQQLVLAMERNA
                     YEHLETFEVLHRSWHVTLRSVRPDQRMVAHTGFITLARRV"
     gene            complement(4564..5784)
                     /locus_tag="VIH70_05655"
     CDS             complement(4564..5784)
                     /locus_tag="VIH70_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8115882.1"
                     /translation="MKVRLRNPDREVELAGGRKIHDLLGELGINPDTVLVIRERELLT
                     REDRVGETDVIEIRPVISGGAGNRMKCRRCKAPAVVEIRRHNAAFCSGCFLRHVREQV
                     KRAIEAHDMFEPTDRILVAVSGGKDSLALWDVLLELGYRADGLYLGLGIGEYSERSHE
                     VARAFARERGAHVVEVDLARDYGFDVPTAGRKGSRSTCAVCGLSKRYVFNRAAREGGY
                     DVIATGHNLDDEVATLLGNTLRWQTDYIARQSPVLPAEEGFARKVKPLYRLSELETAA
                     FAFLRGIDYVVEECPLVGGNTQLRYKEAMNQLESTSPGTKAQFFLGYLDRAAPLFATR
                     DEVRLVGCERCGQPTTGRFCAFCRARAQILGERLEGARDERGSQGRPSDDRETAEALS
                     DEVMPVEIYGTLRR"
     gene            complement(5833..6942)
                     /locus_tag="VIH70_05660"
     CDS             complement(5833..6942)
                     /locus_tag="VIH70_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8115883.1"
                     /translation="MRRALLALGAVAALTLSACTTGSTDSATMPPETNGSPAANFPSS
                     KPVLSSGRDAVVNVVKSVLPAVVNVVVDTGAGQGEGTGFIVRSDGIIVTNYHVVENAS
                     KVRLLTSADDPVEYDARVIGGDVGADLAVLDIDANDLPTVTLGASDALQLGQRVVAVG
                     YALGLEGGPSVTAGIVSSLTRQIDVQDTGCPVSECGADQTRVYDHVIQTDAAINPGNS
                     GGPLLNMEGQVVGINSAGANQAENIGFAIQIDSVKPTIFQAAEHPSQPVAFMGISSGP
                     ASDPQVQFQFAPPVDRGAVIVDVVGGGPADDAGIVVGDTIVRFDGQTVTDSEQLGELI
                     RSHRPGDQVSVGIVHADGSQDEVTVTLGVNPVPTT"
     gene            complement(7071..8000)
                     /locus_tag="VIH70_05665"
     CDS             complement(7071..8000)
                     /locus_tag="VIH70_05665"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY8115884.1"
                     /translation="MAPRSHLHALLQAGEFVVTAELQTSDSADPARVTELAGTLRGKV
                     DAVNCTDNSAAHAHIASIAAARLLLDLGVEPVMQLVCRDRNRLALQADLLGAAALGVR
                     NVVCMTGDDVGAGDHPETKPIYDLDSVQLIRMATIMRDHGTYLSGRALTDAPSLLIGA
                     VENPFAPPLEFRPMRLGTKIEAGAEFIQTQICFNLEKLRLFMSRCGDLGLLDHVWVLA
                     GVFVPSSASGSRYLRDQVPGVDVPAGVIERMASVPPGAQREEGIRIALEIVEAVRAIP
                     GISGIHLMTINHEEAIPRVVEGAGLLPRPTRRM"
     gene            complement(8020..8451)
                     /locus_tag="VIH70_05670"
     CDS             complement(8020..8451)
                     /locus_tag="VIH70_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8115885.1"
                     /translation="MKETGIDRRPPQTAPTAKGFHRAILLVERVTKEAVWDCRMCGQC
                     ILHSTGLSCPMRCPKNLRNGPCGGTLQDGSCEVLPEMKCVWVSAWEGSRRLPLWRDHI
                     QHVEPPVDWRLQGTSSWENLVTGRDRHVPEGWSRSAHGSGS"
     gene            complement(8479..9657)
                     /locus_tag="VIH70_05675"
     CDS             complement(8479..9657)
                     /locus_tag="VIH70_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY8115886.1"
                     /translation="MVNERQEMLEQSATIYFGPWYRRSPFFEATRRAGCTAYDVYNHM
                     LLPAYFDDPVVEYWALLNDVTVWDVAVERTVEISGPDADRFVDSLTCRDLTRCAVKQG
                     KYMIVTAPDGGIVNDPVLLHVGEDRWWMQLADSDAGLYALGAAAQSNLDVEVSYPHAY
                     PMQVQGAKAARTLEKLVGPAVLDLRYYWCDWFDVGDIPVLISRTGWTAVQGFEINLLD
                     PGRGDDLWDAVFAAGEEFGIRPVAPVEARRIEAGIMNYNSDMTHEDTPLHLMGLEHLV
                     EDQPQDYIGKAALEALKARGVDRKLVGIEWSGDELKAELSWFWPVVRDGEQVGRVTDA
                     VWSPRLEKNIGYAWVPIDLAGPGTGLDVESEHGPISVTTAGIPFIDARKEVPAASLRG
                     "
     gene            complement(9801..>10647)
                     /locus_tag="VIH70_05680"
     CDS             complement(9801..>10647)
                     /locus_tag="VIH70_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY8115887.1"
                     /translation="SDGGIINDPVLTRMDETTFWFALASSDALLFARGLKNAYRDLDV
                     TIREADVAPLQVQGPKSKDLMAKLIGPEILDLRYYFWTEAKIDGAPVVITRTGWTSEV
                     GYEVYLTDTTKGVEVYDAIMAAGEEFGIRPTGPSDIRRIEGAIFNWGADMTYENDPLE
                     MGLERLVDWDLSDDACISMRALRTIKEHRVRRRINGVELDGDPFPELNNLKWPVVDGG
                     EEIGRVTSAIYSPRLERNIGFAWLPVGRSTLGERVTVLTEWGPRSATVVEMPFVDPSK
                     QIPVS"
BASE COUNT         1536 a         3810 c         3527 g         1774 t
ORIGIN      
        1 ctcgaggacg gagtcgatct cgtccaggat gtcgtccatc tcctccttga gcttctcgcc
       61 cttggaggcg gaggtcacct ggccggcctc gtcgtccgag ccgccgccgg aacgcttctg
      121 ggtcttcttc tgctcctgct cttgaggcac cgccgagctc cttcccctga acgaccgatt
      181 ctatccggac tccgcgacag cacccatcac cgcgcgggcg atggttctct cccgacgccc
      241 ggccgagcag ggagcagctc ggatccgccc tgcgcgcgag gtcgatccat cgcgcggcct
      301 gaccgaaccg ccggcggcgc gcatacccga tcgccaagat tcacgcctgc agcgccttgg
      361 ggccggccct cgactcccgg gggtcgcgga aggctacgag cggagcatct cgacgagcgc
      421 cgccgcgtcg gcggcctcct cgaacagccg gtcgacgtgc tccctggtgc ctcggagcgg
      481 ctcgcgcatc ggcacacgct gcagcgcctc ggcgcccgtg tcgaagatga tcgagtccca
      541 cgaggccgcg gcgatcgcgt cgggatagcg cgagatgcag cgcccccgga aataggccct
      601 cgtgtcgtcg ggtggctcca tgatcgcccg ctcgacgtcc tcgtcggcca cgagtcgctc
      661 gacctgaccc gtcgcgacca gccggtggta caaccccttg tcacgacgca cgtcgtggta
      721 ttgcaggtcg atgagccgca gcttggcgtc cgaccagtcg agcccgcccc gctcgcggta
      781 gccgtcgagc agccggtact tcgcgaccca gtccagctcc cgggagagct gcatcgggtc
      841 gtcctcgagc ccggtcagca ccgcctccca tcgctcgagc acctcgaggt tgtcctccgt
      901 cgtgtcctcc tgctcgacgt acttccgcgc gcgctcgaga tactcccact gcatctgcac
      961 cgccgtgagc ctgcggccgt cggcaagtcg gaccggcgcc gtgcacgtga ggtcccggga
     1021 gacgtcgtgg agcgacggca ccggcgtcgc cacagcgagg tcggggacgt cgccgtcctc
     1081 gatcagcttc aacacgagcg acgtcgtccc gagtttgagc agcgtcgcga cctcgcacag
     1141 gttcgcgtcg ccgacgatca cgtgcaggcg gcggtacttc tcggggtcgg cgtgcggctc
     1201 gtcgcgcgtg ttgatgatcg gacgcttcag ggtggtctcc aggccgacct cggcctcgaa
     1261 gaagtccgcg cgctgcgtga cctggtaacg gtctcgagac cgctcgtccc acgacgcctc
     1321 gccgccgagc ttgccggccc cgcagaagat ctgccggctc acgaagaacg gcgtgagtcc
     1381 cgcgaccagg ttcgcgaacg gcacctgcct gtcgaccagg tagttctcgt gcgtcccgta
     1441 ggagttgccc ttgccgtcgc tgttgttctt gtagaccgcg agccgctggg caccgggcat
     1501 cgtgcggcgg acctcgtcca gggaccgctc gagcacgcgc tcccccgcct tgtcgtggat
     1561 cacgagttcg cgcggcgtca cgcactcggg tgtcgagtac tcggggtggg cgtggtcgac
     1621 gtagtaacgg gcgccgttgg gcaggatcac gttcgccaga cccagatcct cctcggaggg
     1681 ctcccgcagc tggaccggct cgaacccgcg cgcgtcgcgg agcggcgact cctgctcgta
     1741 gtcccagcgg atccgccgga gcgcgcccgc gtacgtgctg atcagcagcg aggacgagat
     1801 caccgggttg aactcgggag acgcggccgc ggagatcccg tactcggtct cggttccgat
     1861 gaccttgggg atggccatcc ggcgacctta caggtactgg ccggcgctga ctcgctcgac
     1921 ctgcctgccc tcgccgtccc cgtcgccgag cagggttcgc acgtagacga tgcgctcgcc
     1981 cttcttgccc gagatccggg cccagtcgtc ggggttcgtg gtgttcggca ggtcctcgtt
     2041 ctccttgaat tcgtcgcgga tcgcgtggga caggtcctcc gccttgatcc ccttctcccc
     2101 cgtggacagg aagcgcttga tcgcctcctt cttggcgcgg cgcacgatgt tctcgatcat
     2161 cgcgcccgag ttgaagtcct tgaagtacag gatctccttg tcgccgctcg cgtaggtgac
     2221 ctcgaggaac cggttgtcct cggcctccga gtacatccgg tcgaccgtcg actggatcat
     2281 ccgcgtggtc gtcaccttca cgtcgccgct cgcgcgggcg acgtcctcgg ggtggatcgg
     2341 cacgttcggg ttcaggtact tggccatgat gtcggccgcc tgcgcggcgt ccgggcgctc
     2401 gatcttgatc ttcacgtcca gccgccccgg ccgcaggatc gccgggtcga tcaggtcctc
     2461 gcggttcgag gcgccgatca cgatgacgtt cttcagcgtc tcgacgccat cgagctcgga
     2521 caggagctgc ggcacgatcg tcgactcgac gtcgctcgag atccctgttc cacgggtccg
     2581 gaagatagag tccatctcgt cgaagaagac gatgacgggc agcccttcct cgctccgctc
     2641 cttggcccgt tggaagatct ggcggatctg acgctcggtc tcgccgacgt acttgttcag
     2701 aagctcgggc cccttgatgt tcaggaagta cgagtgcgcg tcctggcgcc cggtccgctc
     2761 gcgcaccttg tcggcgagcg acttggcgac ggccttggcg atcagcgtct tgccacaccc
     2821 gggaggcccg tagagcagga cgcccttcgg cgcctcgagc tggtgctcct ggaacagctc
     2881 ggcgtacagg aaggggagct cgacggcgtc gcgtatggcc tcgatctgtc cctcgagccc
     2941 gccgatgtcc tcgtaggaca cgtcggggac ctcctcgagg acgagctcct cgacctcctc
     3001 cttgggcagc ttctcgagca cgacgttgga cctcgggtcg atcagcagcg ggtctccggc
     3061 gcggatctgc tcgtcgagca gcgcggtgcc gagcgtggcc accagctcct cgtcgccgcg
     3121 tccgaccacg atcacgcggt cctcgcccaa ccggtccttg accttgacca cctcgcccgc
     3181 ccggtcgggc agcagcacct cgaccacgtt gagcgcctcg ttgaggatca cctcggcgcc
     3241 cttgcgcagg cggtcggtct cgacgtccgg tgacacgttc acgcgcatct tgcggccggc
     3301 ggtgaacacg tcgaccgagc cgtcgtcgtt gacgccgaga tacaccccga acgacgccgg
     3361 cggctggctg agcttctcga cctcctcacg gagcccctcg atccgctcct tctctgcctg
     3421 cagggtgccg gcgagcttct cgttctgcga gagcgcgcgc gagagatccg aacgcgcctc
     3481 gaggagcttg tcctcgagca gcttgacctg acggggcgcg ttcgtgagtc gccggcggag
     3541 gagggagacc tcgtcctcga ggaagcggac ctgcgtctgg agttcgtgga cctgcttctc
     3601 gaactgttcc cgctgctcgc gtggatcggg ctccggcatc tccgtcacct cccgccggtg
     3661 cggaagcata cctccggtct tcgccgcgac cgacgaagac gggggaggtc tcttggccta
     3721 tgcatacggt cttcggccgg tatggcgctt gacctgagct cttttcagaa gcgacgggcc
     3781 ggcgtgcctt tcagacgcga cgggccagcg tgatgaaccc ggtgtgcgcc accatccgct
     3841 gatccggtcg cacgctccga agggtcacgt gccacgagcg gtgcagcacc tcgaaggtct
     3901 ccaggtgctc gtacgcgttg cgctccatgg cgagcacgag ctgttgcacc tggttcgtcg
     3961 tcgggaggta cccgcagaag atggctcccg ggggcaggac ggtcgagaga ggctcgagcg
     4021 tctgccacgg ctccgggagg tccaggaccg cgcgatcgaa cgtctcgccg cttcccccga
     4081 cgtcgcggac gtcgccctcg cggagctcga gccagtcggg cgccttcccg aagaaggact
     4141 cgatgttgcg cgcggcgccc gtctggaact cgcgccgcag gtcgtacgac accacccggc
     4201 cctcgggccc ggtggcccgg cacagggcga gcgtgagcga ccccgaaccc gtcccggcct
     4261 ccagaacgcg agcgcccggg aagacgtcgg ccccgatcag gatcgcgccg atgtccttgg
     4321 ggtagacgag ctgggcgtcc ctcggcatct tcagcacgaa gtcggccagc cgcggccgga
     4381 acgcccggaa cgccatcccg gtcgccgagt gcacgagggt cccctccggc gacccgatca
     4441 gcaggtcgtg cggaagcccg ccaccgtgag agtgccaggt ctcgccggtc tggagcgtga
     4501 gcaggtaccg acggtcgcgc tggtccacga gcaggatctt ctcccccgcc tcgaacggtc
     4561 cgttcatcga cggagcgtcc cgtagatctc gaccggcatg acctcgtcgg acagcgcctc
     4621 ggcggtctca cgatcgtcgg acggcctccc ctgcgaccct cgttcgtccc gcgccccctc
     4681 gagccgctcg cccaggatct gcgcccgcgc gcggcagaac gcgcagaacc tgcccgtcgt
     4741 cggctgacca cagcgctcgc acccgaccag ccggacctcg tcccgcgtcg cgaacagcgg
     4801 cgccgcccgg tcgaggtagc cgaggaagaa ctgggccttg gtccccggcg acgtcgattc
     4861 gagctggttc atcgcctcct tgtagcggag ctgcgtgttg ccgccgacga gcgggcactc
     4921 ctccacgacg tagtcgatcc cccgcaggaa cgcgaacgcc gcggtctcga gctccgagag
     4981 ccggtacagc ggcttgacct tgcgcgcgaa cccctcctcc gcgggcagca cgggcgactg
     5041 gcgcgcgatg tagtccgtct gccaccgcag ggtgttgccg agcagggtgg cgacctcgtc
     5101 gtcgaggttg tgcccggtcg cgatcacgtc gtacccgccc tctcgcgcgg cccggttgaa
     5161 gacgtagcgc ttggaaagcc cgcagacggc gcaggtcgac cgcgagccct tcctgccggc
     5221 ggtcggcacg tcgaacccgt agtcgcgcgc caggtcgacc tccaccacgt gcgcaccgcg
     5281 ttcgcgtgcg aaggcccgag cgacctcgtg tgaccgctcc gagtactcgc cgatgccaag
     5341 gcccaggtac agcccgtcgg cccggtaacc gagctcgagc aggacgtccc acagcgcgag
     5401 cgagtccttg ccgccggaga ccgcgaccag gatcctgtcc gtgggctcga acatgtcgtg
     5461 cgcctcgatc gcgcgcttga cctgctcgcg cacgtggcgc aggaagcacc cggagcagaa
     5521 cgccgcgttg tgcctgcgga tctcgacgac ggcgggagcc ttgcagcgcc ggcacttcat
     5581 cctgttcccg gcgccgccgc tgatcaccgg gcggatctcg atcacgtcgg tctcgccgac
     5641 ccggtcctcg cgcgtgagca gctcgcgctc gcggatcacg agcacggtgt cggggttgat
     5701 gccgagctcg ccgaggaggt cgtggatctt tcggccgcca gcgagctcga cctcccggtc
     5761 ggggttgcgg agcctcacct tcacgcaggt caggctagcg cacccgcccg ggcaggagcc
     5821 gccgagctcg gatcaggtgg tcgggaccgg gttcacgccg agcgtgaccg tgacctcgtc
     5881 ctgggagccg tcggcgtgga cgatccccac gctgacctga tcgcccggcc gatgcgaacg
     5941 gatcagctcg ccgagctgtt cggagtcggt caccgtctgc ccgtcgaacc gcacgatggt
     6001 gtcgccgacg acgatcccgg cgtcgtctgc ggggccgccg cccactacgt cgacgatcac
     6061 ggcgccccga tccaccggcg gagcgaactg gaactggacc tgcggatcgg aggccggccc
     6121 ggagctgatc cccatgaacg caacgggctg tgacgggtgt tcggcggcct ggaagatcgt
     6181 cggcttgacc gaatcgatct ggatcgcgaa cccgatgttc tcggcctggt tcgcacccgc
     6241 cgagttgatc ccgacgacct gcccctccat gttgagcagc ggtccaccgg agttcccggg
     6301 gttgatggcc gcatcggtct ggatcacgtg gtcgtacacg cgggtctggt cggcgccaca
     6361 ctccgagacc ggacatcccg tgtcctggac gtcgatctgc ctcgtcagcg aggagacgat
     6421 ccccgcggtc accgaggggc cgccctcgag gccgagggcg tacccgaccg cgacgacccg
     6481 ctgcccgagc tgcagggcat ccgaggcgcc gagcgtcacg gtcggcaggt cgttggcgtc
     6541 gatgtccaga accgccaggt cggccccaac gtcgcccccg atcacgcggg cgtcgtactc
     6601 gacggggtcg tccgccgacg tcagcagcct gaccttcgag gcgttctcga ccacgtggta
     6661 gttcgtcacg atgatgccgt ccgagcgcac gatgaacccg gtgccctctc cctgaccggc
     6721 gccggtgtcg acgacgacgt tcaccaccgc cggcaggacg ctcttcacga cgttcacgac
     6781 ggcatccctg ccgctcgaga ggaccggctt cgaggagggg aaattggctg caggagagcc
     6841 gttcgtctcg ggtggcatgg tggcggagtc ggtgcttccg gtggtgcacg cggacagcgt
     6901 cagcgccgct acggcgccga gcgcgaggag ggcacgcctc atggtcatgg ctccagtctg
     6961 tcgggttcag cgggcattcg acacacggca cccccacgtt caccgacgcg gccgaacgtt
     7021 gcctgagagt cacgaacggg cggctggccc gcacgatcgc tacgcgatcg tcacatccga
     7081 cgggtcgggc gcgggagcaa cccggcgccc tcgaccaccc tcgggatcgc ctcctcgtgg
     7141 ttgatcgtca tcaggtggat ccccgagatg ccggggatcg ctcgcaccgc ctcgacgatc
     7201 tcgagcgcga tccggatgcc ttcttccctc tgcgccccgg gggggaccga cgccattcgc
     7261 tcgatcaccc cggcgggcac atccaccccg gggacctggt cgcgcaggta gcggctcccc
     7321 gacgccgagc tcggcacgaa cacccccgcg agcacccaca cgtggtcgag cagcccgagg
     7381 tcgccgcacc gcgacatgaa cagccgcagc ttctccaggt tgaagcagat ctgcgtctgg
     7441 atgaactccg caccggcctc gatcttcgtt ccgaggcgca tcggccggaa ctcgagcggc
     7501 ggcgcgaacg ggttctcgac ggcgccgatc aggaggctcg gcgcgtcggt cagggctcgg
     7561 ccagacaggt aggtgccgtg gtcgcgcatg atggtggcca tcctgatcag ttggacggaa
     7621 tcgaggtcgt agatcggctt ggtctcggga tggtcgccgg cgccgacgtc gtcgccggtc
     7681 atgcacacga cgttgcgaac ccccagcgcg gccgccccca gcagatcggc ctgcagggca
     7741 agccggttcc ggtcgcggca gaccagctgc atcacgggtt cgacccccag gtccaggagc
     7801 agccgcgccg cggcgatcga ggcgatgtgc gcgtgggcgg cgctgttgtc cgtgcagttc
     7861 acggcgtcga ccttcccacg gagggtcccg gcgagctcgg tgacgcgcgc cgggtcggcc
     7921 gagtcgctcg tctggagctc ggccgtgacg acgaactcgc cggcctgcag cagagcgtgc
     7981 aggtggctgc gcggagccac ccccgtcatg gggtgggtct cacgacccgg atccgtgcgc
     8041 ggatcgggac cagccctccg gcacgtgccg atcgcgcccc gtcaccaggt tctcccacga
     8101 cgacgtcccc tgcaggcgcc agtcgacggg cggctccacg tgttggatat ggtcgcgcca
     8161 gagcggcagg cgccgcgagc cctcccaggc ggagacccag acgcacttca tctcgggcag
     8221 cacctcgcag ctcccgtcct gcagcgtgcc gccgcagggg ccgttccgca ggttcttggg
     8281 gcagcgcatc gggcacgaca gccccgtcga gtgcaggatg cactgcccgc acatgcgaca
     8341 gtcccacacg gcctccttcg tgacgcgttc gacgagcagg atggcccgat ggaacccctt
     8401 cgccgtgggc gcggtctgcg gcggcctccg gtcgatcccg gtctccttca tccgaggctc
     8461 ctcggtcggt ccggtggtct acccgcgcag ggaggcggcc gggacctcct tgcgggcatc
     8521 gatgaatggg atcccggcgg tcgtgaccga gatcggcccg tgctcggact ccacgtcgag
     8581 gccggtcccc ggccctgcga ggtcgatcgg gacccacgcg tacccgatgt tcttctccag
     8641 tcggggcgac cagactgcat cggtgacccg gccgacctgc tcgccgtccc gaaccacggg
     8701 ccagaaccac gagagctcgg ccttgagctc gtcgcccgac cactcgatcc ccacgagctt
     8761 tcgatccacg cccctggcct tgagggcctc gagcgccgcc ttgccgatgt agtcctgcgg
     8821 ctggtcctct accaggtgct ccaggcccat caggtgcagc ggcgtgtcct cgtgggtcat
     8881 gtccgagttg tagttcatga tcccggcctc gatgcgccgc gcctcgacgg gcgcgacagg
     8941 ccggatcccg aactcctcgc ccgcggcgaa gaccgcgtcc cacaggtcgt cgccccggcc
     9001 ggggtcgagc aggttgatct cgaacccctg gaccgcggtc caccccgtcc ggctgatcag
     9061 caccgggatg tcgccgacgt cgaaccagtc gcaccagtag tacctgaggt cgagcacggc
     9121 cggtccgacg agcttctcga gcgtcctcgc ggccttcgct ccctggacct gcatcgggta
     9181 cgcgtggggg tacgagacct cgacgtcgag gttcgactgc gccgccgcgc cgagcgcgta
     9241 cagccccgcg tccgagtccg cgagctgcat ccaccatcga tcctcaccga cgtggagcag
     9301 caccggatcg ttgacgatcc ctccgtcggg ggccgtcacg atcatgtact tgccctgctt
     9361 gacggcgcac ctcgtcaggt ccctgcaggt gagcgagtcg acgaagcgat cggcgtccgg
     9421 gcccgagatc tcgaccgtgc gctcgaccgc cacgtcccac accgtgacgt cgttcagcag
     9481 ggcccagtac tcgaccacgg ggtcgtcgaa gtacgccggc aggagcatgt ggttgtagac
     9541 gtcgtaggcg gtgcagcccg cgcgccgcgt cgcctcgaag aacggggatc gtcggtacca
     9601 gggtccgaag tagatcgtcg cgctctgctc gagcatttcc tgccgttcat tcaccatccg
     9661 ggcacgaccc ccttcgatcc cgcggccggg cgccccggga ctttgaatct tcgttcaatc
     9721 gatgctccgc cgcaagcccg gcgccgtccg caggccgcgg tctccggctg aaccgcgctt
     9781 cagtttccgg ggcgagagcc tcaggagacc gggatctgct tgctcgggtc gacgaacggc
     9841 atctcgacca cggtggccga gcgcggaccc cactcggtaa ggacggtgac gcgctcgccg
     9901 agcgtcgagc gcccgaccgg gagccacgcg aacccgatgt tgcgctccag ccggggcgag
     9961 tagatagctg aggtcaccct gccgatctcc tctcccccgt caacgacggg ccacttcagg
    10021 ttgttgagct cggggaaggg gtcgccgtcc aactccacgc cgttgatcct gcggcgcacc
    10081 cggtgctcct tgatcgtacg cagcgctcgc atcgagatgc atgcgtcgtc cgagaggtcc
    10141 cagtccacca ggcgctcgag tcccatctcc agcggatcgt tctcgtaggt catgtcggcg
    10201 ccccagttga agatcgcgcc ctcgatccgg cggatgtcgc tcgggcccgt cgggcggatc
    10261 ccgaattcct ccccagccgc catgatcgcg tcgtacacct cgacgccctt cgtcgtgtcg
    10321 gtgaggtata cctcgtagcc gacctccgag gtccacccgg tgcgggtgat cacgaccggc
    10381 gccccgtcga tcttcgcctc ggtccagaag tagtagcgga ggtcgaggat ctcgggaccg
    10441 atcagcttgg ccatcaggtc cttcgacttc ggcccctgga cctggagcgg cgcgacgtcc
    10501 gcctcgcgga tcgtcacgtc gaggtcccgg tacgcgttct tcagcccgcg ggcgaacagg
    10561 agcgcgtccg aggacgcgag ggcgaaccag aaggtggtct cgtccatccg agtgaggacc
    10621 gggtcgttga tgatgccccc gtcgctg
//
LOCUS       DATBRK010000189         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1619779, whole genome shotgun sequence.
ACCESSION   DATBRK010000189 DATBRK010000000
VERSION     DATBRK010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619779"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VIH70_05685"
     CDS             <1..649
                     /locus_tag="VIH70_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY8115888.1"
                     /translation="LIFGVVSYNRFVSQRQLIKDAWANIDTELRRRYDLIPNLVESVR
                     GYASHERTVFENVTRARAAAASATGSPAAQAAAEGPLVAALRQLFAVVENYPDLKANQ
                     NFLALQQELANTEDRLQTSRRFYNANVRDYNQRVKQFPSVIIASTFNFDEEEFFEIED
                     ALREAGAPQVAFGGEGPGVTFGGPEIPPAAPPPAPTAPTSEPSGTLPPSPRTPDA"
     gene            complement(646..>1597)
                     /locus_tag="VIH70_05690"
     CDS             complement(646..>1597)
                     /locus_tag="VIH70_05690"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8115889.1"
                     /translation="ATAVFAAAFEPASMAAMPNLVDERDLSIANALSGSLWGTMLAVG
                     GALGGVVAGVFGSDVAIVVDSASFVLSALLILGIHATFAETRTVHEPPSLRQDTVETL
                     RYARKDHRVLALLAVKFGWGVAGGVLVLLPILALGRFHAGEIGLGLLMAARGVGALIG
                     PFIGRSLAGPQDRRIFGAIGGALATFGVGYALLGLAPALVFAMGAVMVAHVGGGAQWT
                     LSSYGLQRVVPDRIRGRIFAVDGMLVTLTFGLSAILTGWLADVFTAKTTALAMGGVAL
                     VWAIVWTLLTGDVRRATMLEGCGPAPELLREPDEVSTHQP"
BASE COUNT          262 a          603 c          539 g          193 t
ORIGIN      
        1 cctgatcttc ggcgtcgtct cgtacaaccg attcgtctcc cagcgtcagc tgatcaagga
       61 tgcctgggcg aacatcgaca ccgagctgcg ccgccggtac gacctgatcc ccaacctcgt
      121 cgagtccgtt cgcggctacg cgtcgcacga gcgcacggtg ttcgagaacg tgacgcgcgc
      181 ccgcgcggcg gccgcctcgg cgacgggctc gcccgctgcc caggccgccg ccgaggggcc
      241 gctcgtcgcc gcgctgcgac agctgttcgc cgtagtggag aactacccgg acctgaaggc
      301 caaccagaac ttcctcgcgc tccagcagga gctggccaac accgaggatc gcctccagac
      361 gtcgcgccgc ttctacaacg ccaacgttcg cgactacaac cagcgcgtga agcagttccc
      421 gtcggtgatc atcgcgtcga cgttcaactt cgatgaagag gagttcttcg agatcgagga
      481 cgcgctccgc gaggcgggcg ccccgcaggt ggctttcggc ggcgaaggac ccggcgtcac
      541 gttcggcggg cccgagatcc cgccggccgc accgccgccc gcgccgacgg cgccgacgtc
      601 ggagccctcg gggacgctgc ctccgtcgcc ccggacgccc gacgcctagg gctggtgggt
      661 cgagacctcg tctggctccc gtaggagctc gggagcgggg ccgcacccct cgagcatcgt
      721 cgcgcggcgg acgtcgccgg tcagcagtgt ccacacgatc gcccagacga gcgcgacgcc
      781 gcccatcgcg agggccgtcg tcttcgcggt gaacacgtcg gcgagccagc ccgtcaagat
      841 cgccgacagg ccgaacgtca gcgtcacgag catgccgtcg acggcgaaga tgcggccccg
      901 gatccggtcg ggcacgacgc gctggagccc gtacgacgac agcgtccact gggcgccgcc
      961 gccgacgtgc gcgaccatga ccgcccccat cgcgaacacg agagccggcg ccaggccgag
     1021 gagcgcgtag ccgacgccga acgtcgccag cgcgccgccg atcgcgccga agatccgacg
     1081 gtcctgtggg ccggcgaggg accttccgat gaacgggccg atcagcgccc ccacgccgcg
     1141 cgccgccatc agcagcccga gcccgatctc gcccgcgtgg aacctgccga gcgccaggat
     1201 cggcaggagc acgagcacgc cgccggcgac cccccagccg aacttcaccg cgagcagcgc
     1261 gaggacgcgg tggtccttgc gcgcgtatcg cagggtctcg accgtgtctt ggcgcagact
     1321 gggcggctcg tggaccgtcc gggtctcggc gaaggtggca tggatcccga ggatcaacag
     1381 ggccgagagc acgaacgatg cggagtccac gacgatcgcg acgtcggatc cgaacacccc
     1441 cgccacgacc ccgccgaggg caccgccgac cgcaagcatc gtgccccaca gcgaccccga
     1501 cagggcgttc gcgatcgaga ggtcgcgctc gtcgaccagg ttcggcatcg ccgccatcga
     1561 ggccggctcg aacgcggccg cgaacaccgc ggtcgcg
//
LOCUS       DATBRK010000190         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1763631, whole genome shotgun sequence.
ACCESSION   DATBRK010000190 DATBRK010000000
VERSION     DATBRK010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763631"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VIH70_05695"
     CDS             complement(<1..165)
                     /locus_tag="VIH70_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017547025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8115890.1"
                     /translation="MAGIVLDQVSKVYADGTTAVSDLSLEVRDEEFIVLVGPSGCGKT
                     TALRMVAGLES"
     gene            complement(240..830)
                     /locus_tag="VIH70_05700"
     CDS             complement(240..830)
                     /locus_tag="VIH70_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8115891.1"
                     /translation="MVDIAGSIESLLLPHPAVRGVELVGSRADGSPTPLSDWDFVVVT
                     NRFYDVARALPELVTPLDPLSQQWDRISDYPCFMLMLEGPVKVDLIFPEEPYESLPPW
                     TVSADTLDGIDQHFWDWILWLASKREKGQDQLVQGELVKMRDHLLRPMGVDRVRGSIE
                     AAVARYRSARDEWERELGIEVSRRREREVLSVLPDA"
     gene            855..1367
                     /locus_tag="VIH70_05705"
     CDS             855..1367
                     /locus_tag="VIH70_05705"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019328603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEY8115892.1"
                     /translation="MPKIVLLEGDLTTQDVDAIVNAANRSLLGGGGVDGAIHRAGGPS
                     ILAECREIRRTTFPDGLPTGRAVATGGGDLAARWVIHTVGPVYAASGDPPGELASCHT
                     ESLRVADELGARTVAFPAISTGVFGYPVHEAASVAVGAVRGAETQVEEVRFVLFGREA
                     FDAFEQALGR"
     gene            1419..1763
                     /locus_tag="VIH70_05710"
     CDS             1419..1763
                     /locus_tag="VIH70_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8115893.1"
                     /translation="MSDVATDLTTSSGLAEHLDDVQVVDCREPYEWQAGRIEGSIHIP
                     LNTILAGETGELDSTKTTVVVCRSGNRSELATLMLRARGFDAYNLGHGLEEWADEGRP
                     LTTPDGAAGHVA"
     gene            complement(1768..2205)
                     /locus_tag="VIH70_05715"
     CDS             complement(1768..2205)
                     /locus_tag="VIH70_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115894.1"
                     /translation="MATWAEFERADAQLAAFGRERLDGLVCFHATLRRDGSPRVHPVE
                     PWVAAGLLMVRFRRQSPKVREVGRDARYALHSPMDNPDGVGGEFLVRGWMEQISPDHP
                     AAIEIARSTRTPLAFYALSVEEAVGTIYEGADRDPVYLRWRSS"
     gene            2228..3691
                     /locus_tag="VIH70_05720"
     CDS             2228..3691
                     /locus_tag="VIH70_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8115895.1"
                     /translation="MADVEGARRALEDALGAGEVLSDPLALRLYARDASMVEGGCALV
                     AFPRSRDDIVACVRVAAEHELPVVPRGSGTGLAGGSTPIGDALVVVTTKMTDIIEIRP
                     ADRLAWLEPGVPNLDLANALRPHGFTFAPDPSSQQTSSIGGNVSTNAGGPHCLAYGVT
                     SNHVLALDVVLTDGAVVRLGSEGPEAAGYDLRAVMVGSEGTLGIVSSVCVRLTPLAPV
                     THTMLFDFTSVADCAGAVTRIIADGVVPAALEMMDQGIVVAAERFAHAGYPTDAAAIL
                     IVEVDGTAAAVEAQTVVVEAAAEAHRARSVRVAADEAERALIWKGRKSAFGAVATIAP
                     HYHLHDCVVPRTRLVEVLTGVYEIAERHELVVTNVFHAGDGNLHPLLSFDRGVPGTLE
                     RVLRASEQIVRLCVDAGGALSGEHGIGLEKRDFMPLVFSAEDLTAQACVRSAFGAEGR
                     MNPRKVLPDGARCGDFAMARGSDAGAVAEDLPEGAWI"
     gene            3682..4731
                     /locus_tag="VIH70_05725"
     CDS             3682..4731
                     /locus_tag="VIH70_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8115896.1"
                     /translation="MDLIHPPSKAGVVEAVRDANATARRLLVVGGRRHVDKGNPAEVD
                     AELWTTQLDGLVAYEPAEMIAVVEAGMRIGELDGILAAGGQEWPSDAPADATVGGVLA
                     SGASSPRRLRVGALRDTVLEAEIVTGDGRLVRSGARTVKNVTGYDLHKLVVGSLGTLG
                     VIVQVAIKVRPLPHARRTLRFAGDLPLARELVDAVHGSTAVLATPGAVELRLEGWPQE
                     VEEQTTSARGVSDATAQDDASFPLERPWEAMPVVVEAAVPPSRLDATIVGDAWGALAG
                     VGLAWVGLGWPNGELVQVRDRVRAVGGIAPVVRGPGGLGDSPVAAPEVQRRLKLAFDP
                     NGVLAPGRGWEGPEA"
BASE COUNT          670 a         1679 c         1741 g          662 t
ORIGIN      
        1 gctctccagg ccggcgacca tcctcagcgc ggtcgtcttg ccacacccgg agggaccgac
       61 cagcacgatg aactcctcgt cccggacctc gagcgacagg tcggagaccg ccgtggtgcc
      121 gtcggcgtag accttcgaga cctggtccag cacgatcccg gccatcagct cctcctcggg
      181 tcccggcctg ggcccattca accgcagagg gcgcgccgca gtcgagtcga cacttcggct
      241 caggcgtccg gcaggaccga gagcacctcg cgctcgcggc gccgcgagac ctcgatgccg
      301 agctcgcgct cccactcgtc ccgggcggac cgataccgtg cgaccgccgc ctcgatggac
      361 ccgcggaccc gatccacacc catcggccgg agcaggtggt cgcgcatctt gaccagctcg
      421 ccctgcacga gttggtcctg ccccttctcg cgcttggacg ccagccacag gatccagtcc
      481 cagaagtgct ggtcgatgcc gtcgagggtg tcggcgctca cggtccacgg cggcagggac
      541 tcgtacggct cttcggggaa gatcaggtcg accttgacgg ggccctcgag catgagcatg
      601 aagcaggggt agtcgctgat ccgatcccac tgctgcgaga gggggtcgag cggcgtgacc
      661 agctcgggga gtgcgcgagc cacgtcgtag aaacggttgg ttaccacgac gaaatcccag
      721 tccgacaacg gggtcggcga gccgtcggcg cgcgatccaa ccagctcgac cccccgaacg
      781 gccgggtgtg ggagcagcag ggactcgatc gatccggcga tgtccaccat gggtcgacaa
      841 cctacgatgt ggccatgccg aagatcgtgc tcctcgaggg cgacctgacg acgcaggacg
      901 tcgacgcgat cgtcaacgcg gcgaaccgct ccctgctggg agggggcggc gtcgacggcg
      961 cgatccaccg ggccgggggc ccctcgatcc tcgccgaatg ccgagagatc cggcggacca
     1021 cgttccccga cggactgccg acgggccgag ccgtggccac cggtgggggc gacctcgccg
     1081 cgcgctgggt gatccacacc gtcggacccg tctacgccgc atccggtgat ccccctggcg
     1141 aactggcctc ctgtcacacg gagtcgctcc gggtcgccga tgagctcggg gcgcggacgg
     1201 tagcctttcc cgcgatctcg acgggggtgt tcgggtaccc ggtccatgag gcggcgagcg
     1261 tcgcggtcgg agccgttcgc ggcgccgaga cccaggtcga ggaggtccgg ttcgtgctct
     1321 tcgggcggga ggcgttcgac gcgttcgagc aggcgctcgg gcgctgagcg cacgttcttc
     1381 cggtcgttcc gcaccccgct gctaggattc gcatcgacat gagcgacgtg gcgacggacc
     1441 tgacgacctc gagcggtctg gccgagcacc tggacgacgt gcaggtggtc gactgccgcg
     1501 agccgtacga gtggcaggcg ggccggatcg agggctcgat ccacatcccc ctgaacacga
     1561 tcctcgcggg cgagaccggc gagctcgact cgacgaaaac cacggtcgtg gtctgccgct
     1621 ctggcaaccg cagcgagctc gcgaccctga tgctgcgggc tcgcgggttc gacgcctaca
     1681 acctcgggca cgggctcgag gagtgggccg acgagggccg gccgctcacg acgccagacg
     1741 gcgccgccgg tcacgtcgcc tgatcgctca gctcgaacgc caacgcaggt agaccgggtc
     1801 gcggtccgcg ccctcgtaga tcgtgcccac ggcctcctcc accgagagcg cgtagaacgc
     1861 gagcggcgtc cgtgtacttc gagcgatctc gatcgccgcg ggatggtcgg gcgagatctg
     1921 ctccatccat ccgcggacga ggaactcgcc gcccacgccg tccgggttgt ccatcggcga
     1981 gtgcagcgcg taccgagcgt cgcggccgac ctcccgcacc ttcgggctct gccgacggaa
     2041 ccgaaccatc aggagccccg ccgcaaccca tggctcgacg ggatggacgc gggggctgcc
     2101 gtctcgacgc agggtcgcgt ggaagcagac gaggccgtcc agccgctcgc gtccgaacgc
     2161 ggccagctgc gcgtccgccc gctcgaactc cgcccacgtc gccacccgct aggcctaccc
     2221 taggaacgtg gccgatgtcg agggcgcccg ccgagcgctc gaggacgcgc tcggcgccgg
     2281 cgaggtgctc tcggacccgc tggcgctccg cctctacgcg cgtgacgcct cgatggtcga
     2341 gggcggctgc gcgctcgtcg cgttcccccg gtcgcgcgat gacatcgtcg cgtgcgtccg
     2401 tgtcgcagcc gagcacgaac tgccggtcgt cccccggggt tcggggacgg ggctggccgg
     2461 cggctcgacg ccgatcggcg acgccctggt cgtcgtgacg acgaagatga cggacatcat
     2521 cgagatccgg ccggcggacc gcctggcctg gctcgagccc ggcgtcccga acctcgacct
     2581 cgcgaacgcc ctgcgcccgc acgggttcac gttcgccccc gatccgtcct cccaacagac
     2641 ctcgtcgatc ggggggaacg tgagcacgaa cgcgggcggc ccgcactgcc tcgcgtacgg
     2701 cgtgacgtcg aaccacgtcc tggcgctcga cgtcgtcctg acggacggcg cggttgtacg
     2761 gctcgggtcc gagggtccag aggcggccgg ctacgacctg cgcgccgtca tggtgggaag
     2821 cgagggcacg ctcgggatcg tgtcgtcggt gtgcgttcgg ctcacgccgc tcgcgccggt
     2881 gacgcacacg atgctgttcg actttacctc ggtcgccgat tgcgccggcg ccgtgacccg
     2941 gatcatcgcc gacggcgtcg tgcccgcagc gctcgagatg atggaccagg gcatcgtcgt
     3001 tgcggcggaa cggttcgcgc acgccggcta ccccacggac gcggccgcga tcctgatcgt
     3061 ggaggtcgac ggcaccgccg ctgccgtgga ggcacagacg gtggtggtcg aggctgcggc
     3121 cgaggcgcac cgcgcgcgca gcgtccgggt cgccgcggac gaggccgaac gcgccctgat
     3181 ctggaagggc cgcaagagcg cgttcggcgc ggtcgccacg atcgcgcccc actaccacct
     3241 gcacgactgc gtcgtgccca ggacgcggct cgtcgaggtg ctcaccggcg tgtacgagat
     3301 cgccgaacga cacgagctgg tggtcaccaa cgtctttcac gccggcgacg ggaacctgca
     3361 tccgctcctc tcgttcgacc ggggcgtgcc cgggacgctc gaacgcgtcc tgcgcgcgag
     3421 cgagcagatc gtccggctgt gcgtcgacgc aggcggggcg ctctcgggtg agcacgggat
     3481 cgggctcgag aagcgcgact tcatgccgct cgtgttctcc gccgaggacc tcaccgcgca
     3541 ggcgtgcgtt cggtcggcgt tcggcgccga ggggcggatg aacccgcgga aagtgctgcc
     3601 ggacggggcc cgatgcggcg acttcgcgat ggcccggggc tcggacgccg gcgccgtcgc
     3661 cgaggacctt cccgagggcg catggatctg atccatccgc cgtcgaaagc gggcgtcgtg
     3721 gaggcggttc gggacgcgaa cgcgaccgca cgccgcctgc tcgtggtcgg aggtcggcgg
     3781 cacgtggaca agggcaaccc ggccgaggtc gacgccgagc tgtggacgac ccagctcgac
     3841 gggctggtgg cctacgagcc cgcggagatg atcgcggtgg tcgaggccgg gatgcggatc
     3901 ggcgagctcg acgggatcct ggccgccggc ggccaggaat ggccgagcga cgctcccgcg
     3961 gacgcgaccg tcggcggcgt gctcgcgagc ggcgcgtcga gcccccggcg gctaagggtc
     4021 ggcgcgctcc gcgatacggt cctcgaggcc gagatcgtca cgggggacgg ccggctggta
     4081 cgcagcggcg cccggaccgt gaagaacgtg acggggtacg acctccacaa gctggtcgtc
     4141 ggctccctcg ggacgctcgg cgtgatagtg caggtcgcga tcaaggtgcg accgctcccc
     4201 cacgcgcggc ggacgctccg gttcgcgggc gacctcccgc tcgctcgcga gctcgtggac
     4261 gccgtgcacg gttcgaccgc cgtcctcgcg acgccgggcg cggtcgagct tcggctcgag
     4321 gggtggccgc aggaggtcga ggagcagacc acgagcgctc ggggcgtttc cgacgccacc
     4381 gcccaggacg acgcgtcgtt cccgctcgaa cggccgtggg aagcgatgcc cgtcgtcgtg
     4441 gaggccgcgg tccccccctc gaggctcgac gcaacgatcg tcggcgacgc ctggggcgcg
     4501 ctcgccgggg tggggctcgc gtgggtcggg ctggggtggc cgaacggcga gctcgtgcag
     4561 gtccgcgacc gcgtccgcgc ggtcggtggg atcgcgccgg tcgtccgcgg cccaggtggt
     4621 ctgggggact cgcccgtcgc cgcgccggag gtgcagcgcc ggctgaagct cgcgttcgat
     4681 ccgaacgggg tgctcgcgcc gggccggggc tgggaaggcc ctgaggccta ggcgaacgcc
     4741 ttgcggagcc gg
//
LOCUS       DATBRK010000191        10143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1620669, whole genome shotgun sequence.
ACCESSION   DATBRK010000191 DATBRK010000000
VERSION     DATBRK010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620669"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VIH70_05730"
     CDS             <1..268
                     /locus_tag="VIH70_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017609105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEY8115897.1"
                     /translation="PCSALDPIATQRIEDLMQELLSDYTIVIVTHNMQQAARVSDRTA
                     FFTVEVLGDAGHRTGTIVEYDMTETIFTNPSDARTEDYVTGRFG"
     gene            268..960
                     /gene="phoU"
                     /locus_tag="VIH70_05735"
     CDS             268..960
                     /gene="phoU"
                     /locus_tag="VIH70_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY8115898.1"
                     /translation="MTRPHFHEELEKRELRLLALGELAGNAVQRAVEAVMEHDDDAAD
                     AVVAGDDEIDAIYLELDRDMLALLALQAPVAADLRLVSVIMHSSLHLERIGDQAVNVA
                     KVYRISSNLPRNAAIVQQIGEMGHIVVDMVRVAMDALRRRDLELCMRLPKMDDPVDRL
                     NRNMHFEVAKLADDPKALDWGMHMNLAARALERVGDNAVDIGEQVSFLVSGEFREFTD
                     ASHPGGVSSDAT"
     gene            950..1648
                     /locus_tag="VIH70_05740"
     CDS             950..1648
                     /locus_tag="VIH70_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002858071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8115899.1"
                     /translation="MQPDGTRILVVEDEESLADSVRYNLEREGYSVSVAFDGRRALER
                     FRTDPPSLVILDLMLPEVSGLDVCRTIRSDSDVPIIMVTAKDSEADKVAGLELGADDY
                     VTKPFSMRELVSRVRANLRRTRHHAVPAPADEVLEGGPVRMDVARHEVAVREDTVSLP
                     PKEFELLEAFLRRKGRLLTRDFLIEEVWGPDYFGDTRTLDVHVKRLRRKLEADPHQPD
                     HLLTVRGLGYKFVD"
     gene            1721..3787
                     /locus_tag="VIH70_05745"
                     /pseudo
     CDS             1721..3787
                     /locus_tag="VIH70_05745"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
     gene            3784..5298
                     /locus_tag="VIH70_05750"
     CDS             3784..5298
                     /locus_tag="VIH70_05750"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEY8115900.1"
                     /translation="MSTKAASTNLEREAKLQAPAGFRLPELGGDGLVATGMEPQRLVT
                     VYVDTPDLRIARWGSSLRHRQGEGEEKGWTVKLPSPTNGSQLVRTEVNFEGDDARKLP
                     TAAADLLRAYVRGQELAPVARLQTVRRGVEIGDDEGRRLAVVTDDEVSVMDGRRVASR
                     FRELEVELDPQADEGLSEILIDRLKQAGAGPLDNVPKLVRALGPRAASPPDVEVAELD
                     EDATVADVVRGELATSVVRLLRHDAGVRLGEDPEAVHQARVATRRVRSALRTFRDVLD
                     PEWGRSLRDRLKGVADALGAVRDAEVMRDRLRSREPALPGGDRTGLEELVTMLESTRT
                     EARDQLLGAIREETYVDLLDELVEGAREPRVLEDAAGAPAASELRPALERPWQHLEHA
                     VEGAREDASDASLHAVRIRAKRARYAAEAVSPAFGKRAQAFAKAAADLQDVLGDHQDS
                     VVARAWLRQAAEGGADAFVAGELAAIEAQAAAEARAAWPKAWKALSRKRLRFWA"
     gene            5295..6167
                     /locus_tag="VIH70_05755"
     CDS             5295..6167
                     /locus_tag="VIH70_05755"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8115901.1"
                     /translation="MSRPVRAAGGIVLRGDGGDRSVAVVHRPRYDDWSFPKGKLEDGE
                     DEATAALREVEEETGLRCHLGPSVGAVTYRDRRGRLKVVRYFQMDADGGGFAPNREVD
                     ELRWVAVEDAMRLLTYPHDRRMLRRVATGAPASASALYVVRHAKAGIREAWSGPDEER
                     PLTRRGRKQARRLVGWFRDLEIDRVLTSPFVRCLQTVEPLADARGLPVEVATELREGA
                     SVDELLRALATFGDRPTVVCGHGREIRSMIDRIEAGGATIEGARGIAKGSVWVLDREG
                     ERIVAAHYLPAPPG"
     gene            6171..7721
                     /locus_tag="VIH70_05760"
     CDS             6171..7721
                     /locus_tag="VIH70_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEY8115902.1"
                     /translation="MASDTRFAVLDLGSTTFQLLVADADTQGSLTPVFRDRVVLNLGR
                     VLAGRGRIPEDVAGRAAETARRLADLAVRMGADHVVPLATSALRDSPDRDALASLLEG
                     AVGTEVRFIDGREEARLTFAGVAASVALGQGPTLLLDLGGGSLEVALAGPDGLRWGRS
                     LPIGAGRMTGLLVTRDPPTRDERKAVRAAVQETVSPLVEEVAAAEPARCVASGGTAGA
                     LARVLAARRWGAPPASLNQYEVTVSELRDLTRELASLSLAERLRVPGVDERRAELLPA
                     GGWILTSAAAELGAKRLVHSEWGLREGAVLDALGLADRPIPTPGELRRRSVERLVRAW
                     GENRRHVDVVARIAQRLFDDTPELHGLGVLERELLGHAARLHEIGARISPARLHKHGA
                     YLVENAGLRGFTPDEIAMIASLVRFHRGKDPRPVYPAFAALSDAEREAVVLLVGLLRI
                     AHAIGRGPEGDRLEVATDPRDGTVNIVISGSDNPDAAVAEARSAAALLARTLGGPIEI
                     GVGIAGRR"
     gene            complement(7742..8509)
                     /locus_tag="VIH70_05765"
     CDS             complement(7742..8509)
                     /locus_tag="VIH70_05765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8115903.1"
                     /translation="MADLEPLLRFWRALDDLFERTEARWWGAVVSDARFPAIQEPNYA
                     RVETRQPVRLEEIEGSLLPAMRASGSERSHVVVFHPEDQTDLIVAASTRGERIVWDLV
                     MVAAGREVEPMGEVEEVSAFDGPFWSDHRDSARLFDISDADTLDQLQTMERDVLIPAG
                     RRWFSVIDGGRRVALAALLVLERTGFVDHVVTFPYARRRGHAEMLTRRCVSEATEMGA
                     DRVYLLAEPDGDAVRIYDRIGFERVGYLASWLAPRDA"
     gene            8593..9579
                     /gene="corA"
                     /locus_tag="VIH70_05770"
     CDS             8593..9579
                     /gene="corA"
                     /locus_tag="VIH70_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY8115904.1"
                     /translation="MITFRVYHDGSLKREMPFETRTIEEAREGDQRVWIDVVDPTDDE
                     LTELQARLDLHELAIDDSRHWGQRAKVDFYPQHLFLVAHGIDQDERGEIVDREVHLFA
                     GQGLYVATVRREPRFDFAKVATRLESAPDLSKEGIGFLLYLMLDEIVDGYLDVIERFE
                     DAADDIEDDVATEEEGAEGEATRTLARRIFSLRQQVVRFRRLATPMREVVDLLIETPA
                     IVTSALAPYFRDVLDHVIRMTELSDNVRDLLTSARELQLAQVANRQNVVMKQLTAWAA
                     IILIPTLIAGIYGMNFRHMPELEWAFGYPFALALMGVAAFLLYRSFRKREWL"
     gene            9608..9910
                     /locus_tag="VIH70_05775"
     CDS             9608..9910
                     /locus_tag="VIH70_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115905.1"
                     /translation="MAADRSALAELKRAAVSAEWELAPGAMTEDDAIRQLHGDRPHVL
                     VVFGSFPTLVERALEAYPALRVISDRPVPGAVTVGSLEQVRSAVKPSNRSGGPVRA"
BASE COUNT         1463 a         3539 c         3678 g         1463 t
ORIGIN      
        1 gccctgctcg gcgctcgacc cgatcgccac gcagcggatc gaggacctga tgcaggagct
       61 cctgtcggac tacacgatcg tgatcgtgac gcacaacatg cagcaggcag cccgcgtctc
      121 ggaccgcacc gcgttcttca cggtggaggt gctcggggac gcggggcacc ggaccggcac
      181 gatcgtcgag tacgacatga ccgagaccat cttcacgaac ccgagcgatg cgcgcactga
      241 ggactacgtc acagggaggt tcggctgatg acacggccgc acttccacga ggagttggag
      301 aagcgggagc tgcggctgct cgcgctgggc gagctggccg gcaacgccgt gcagagggcc
      361 gtggaggccg tcatggagca cgacgacgac gcggccgacg ccgtggtcgc gggcgacgac
      421 gagatcgacg cgatctacct cgagctcgac cgcgacatgc tcgcgctcct tgcgctgcag
      481 gctccggtgg ccgccgacct tcgcctcgtc tcggtgatca tgcactcgag cctgcacctg
      541 gagcgcatcg gcgatcaggc cgtgaacgtc gcgaaggtgt atcggatcag ctcgaacctc
      601 ccgaggaacg cggcgatcgt ccagcagatc ggcgagatgg ggcacatcgt cgtcgacatg
      661 gtccgggtgg cgatggatgc cctccggcgg cgcgacctgg agctctgcat gcggctgccg
      721 aagatggacg acccggtcga ccggctcaac cgcaacatgc acttcgaggt ggccaagctc
      781 gccgacgacc cgaaggccct cgactggggc atgcacatga acctcgcggc ccgcgcgctc
      841 gaacgcgtcg gcgacaacgc ggtcgacatc ggcgagcagg tctcgttcct ggtgtccggg
      901 gagttccgag agttcaccga cgcgtcccat ccggggggcg tgtcctccga tgcaacctga
      961 cggcacgcgc atcctggtcg tcgaggacga ggagtccctc gccgactcgg tgcgctacaa
     1021 cctcgaacgc gaggggtact cggtctcggt cgcgttcgac ggccggcgag ctctcgagcg
     1081 cttccggacc gatccgccgt cgctggtgat ccttgacctc atgctgcccg aggtctctgg
     1141 cctggacgtg tgccggacga tccgatcgga ctcggacgtc ccgatcatca tggtcacggc
     1201 caaggactcc gaggccgaca aggtcgcggg gctcgagctg ggtgccgacg actacgtcac
     1261 caagccgttc tcgatgcgcg agctggtctc gcgcgtgcgg gccaacctgc gtcggaccag
     1321 acaccacgcg gtgccggccc cggccgacga ggtcctcgag ggggggccgg tccggatgga
     1381 tgtggcgcgc cacgaggtgg ccgtccggga ggacacggtc tcgctcccgc cgaaggagtt
     1441 cgagctgctc gaggcgttcc tgcgccggaa ggggcgcctc ctgaccaggg acttcctgat
     1501 cgaggaggtc tggggccccg actacttcgg cgacacgcgg accctcgacg tgcacgtgaa
     1561 gcgccttcgt cgcaagctcg aagccgatcc ccaccagccc gaccacctgc tcaccgtgcg
     1621 ggggctgggg tacaagttcg tagactgagc cccgcgggct tggcccgtcg gttggcctga
     1681 gggataatcc ctcccttcat ccaaggagtc tggaacggac gcttccccaa ccgcggatct
     1741 cgccgatgac gctcggtacc tgaaccgcga gctgtcctgg ctggagttca acgcccgcgt
     1801 gctcgcgctc gccgaggacg cctcgctccc gctgctcgaa cgggtcaagt tcgtcgcgat
     1861 cttcgcgaac aacatggacg agttcttcca ggtccgcgtc tcgggcctgc aggagcaggt
     1921 cgaggccggg gtggtgaagc gatcgcccga cggcaggacg cccgccgagc agctcgaggg
     1981 gatccgcgag cgtgcgcagg agctgtcgac ccgcgcggcg acggtgttcg ccaccgagct
     2041 cgtgcccgcg ctcgagaagg agcggatccg gatcgtgcgc tcgttcgacg cgctggaccc
     2101 ggcggaccga gcgttcctcg agcaggagtt cgacgagcgc atcttcccgg tgctgacccc
     2161 gctctcggtc gaccccgccc atcccttccc gtacatctcg aacctgtccc tgaacctcgc
     2221 ggcgatggtg cgtgacccga tgacgggcgt tcgcaggttc gcgcgcgtga aggttccacc
     2281 gctgctcccg cgcttcgtcc cgctccccga cggcgagcgg ttcgtcccgc tcgagaccgt
     2341 gatcgccgcg cacctggatc ggctcttccc ggggatggag ctcgtcgcgc atcacgcgtt
     2401 ccgcctgacg cgcgacgccg atctcgaggt cgaggaggac gaggccgagg atctgctcga
     2461 ggcgatccag agcgtgctcc gccgccgccg gcgcggcgcg aacccggtcc gactggaggt
     2521 cgacgagacg atgagccacg aggtgctcga gctccttcgc cgcgagctcg atctggagga
     2581 gcaggaggtc gtcgtgacgc ccgggctgct cgacctgagc gccacgtggt cgctggtcga
     2641 gctcgaccgc cccgagctga agcacgagcc ctgggtccct gtcacgcagc cgcgtctgtc
     2701 gaacggcgac ggtccacccg atctgttcca tgtgctccgg gcgggcgacg tcctcgtcca
     2761 tcacccgtac gactccttct cctcttcggt tgaggccttc gtcgagcagg ccgcccacga
     2821 tccggccgtc ctcgcgatca agcagacgat gtaccgcacc tcgacgcagg agagcccgat
     2881 catccgcgcg ctgatccgcg ccgcggagct cggcaagcag gtggtggcac tggtcgagct
     2941 caaggcccgg ttcgacgagg aggccaacat cacctacgcg cgggagcttg agcaggcggg
     3001 cgtgcacgtc gtccacgggg tcgtcgggct caagacgcac gcgaagatct cgctcgtcgt
     3061 tcgacgcgag gccggggggg tccgcaggta cgcccacgtc ggcaccggca actacaaccc
     3121 ggtgaccgct cggctgtacg aggacatggg gctcctgacg gcagacgccg agatcggcgc
     3181 cgacctcacc gacctgttca acctgctgac cggctacagc cggcagcgcc agtaccgccg
     3241 gctgctggtg gccccggagt acctgcgtcc ggagatcgtg gatctgatcc ggggccaggc
     3301 ccgggagggc ggccggatcg tgttgaagat gaacgcgctc gttgaccccg acatggtcga
     3361 cgccctgtac gaggcctccc gggcgggcgc cgagatcgac ctgatcgtcc gggggatctg
     3421 ctgcctgcgt ccgggtatcc ccggcctctc ggagcggatc accgtccgtt cgctcgtcgg
     3481 ccggtacctc gagcactcca ggatcttccg gttcggcgag cggcgcgggg caacccacta
     3541 catcggttcg gccgacctga tgcagcggaa tctcgaccgg cgggtggagg cgctcgtgcc
     3601 ggtctcggac ccgtcgctgg cggctcggct cgacgagatc ctggacgtgc tgatgcagga
     3661 cgacatgctc tcgtggacgc tcggctcgga cgggacgtgg accaaggttc gcggcgagcg
     3721 cggcatcgac gcgcagcagc ggctccagga gctggcggtc gagcgcgcgg ggtcgcggaa
     3781 ggcgtgagca ccaaggccgc gtcgacgaac ctggagcggg aggccaagct ccaggcgccc
     3841 gcaggtttcc gcctccccga gctgggaggc gacgggctgg tcgcgaccgg gatggagccc
     3901 cagcggctcg tcacggtcta cgtggacacc cccgatctcc ggatcgctcg gtgggggagc
     3961 agccttcgac accggcaggg cgagggcgaa gagaagggtt ggacggtcaa gctcccctca
     4021 ccgaccaacg gatcacagct cgtccgcacc gaggtgaact tcgagggcga cgacgcgagg
     4081 aagctgccga ccgccgccgc cgaccttctc cgcgcctacg tccgcgggca ggaactggca
     4141 ccggtggctc ggctgcagac cgtccgccgg ggcgtcgaga tcggcgacga cgagggcagg
     4201 cggctggccg tggtgaccga cgacgaggtg tcggtgatgg acgggcgccg agtggcgagc
     4261 aggttccggg agctggaggt cgagctcgac ccgcaggccg atgagggcct ctcggagatc
     4321 ctcatcgacc ggttgaagca ggccggcgcc ggccccctcg acaacgtgcc gaagctcgtg
     4381 cgagcgctcg gtccgcgcgc cgcctcgccg ccggacgtcg aggtggccga gctcgacgaa
     4441 gacgccaccg tggccgacgt ggtccgcggg gagctcgcga cgtccgtcgt gcgtcttctc
     4501 cgccacgatg ccggcgttcg tctgggggag gatcccgagg ccgtccacca ggcgagggtc
     4561 gccacgcgtc gagtccgctc ggcgctgcgg acgttccgcg acgtgctcga ccccgagtgg
     4621 ggtcgatcgc tgcgggaccg cttgaaaggt gtggccgatg cgctcggggc cgtgcgcgat
     4681 gccgaggtca tgcgcgaccg gctccgttcg agggagccgg cgctgcccgg gggcgaccgc
     4741 acgggcctcg aggagctcgt gacgatgctc gagtcgaccc ggaccgaggc ccgggatcag
     4801 ctgctggggg cgatccggga ggagacgtac gtggacctgc tcgacgagct cgtcgagggc
     4861 gcccgcgaac cacgggtgct cgaggacgcg gccggtgcgc ccgcggcgag cgagctgcgg
     4921 cccgccctcg agcggccctg gcaacatctc gagcacgcgg tggaaggggc tcgggaggat
     4981 gcatcggacg cctccctgca cgccgttcgg atccgagcca agcgggctcg gtacgcggcc
     5041 gaggccgtgt ccccggcgtt cggcaagcgc gcccaggcgt tcgcgaaggc cgcggccgac
     5101 ctccaggacg tgctcggcga ccaccaggat tcggtcgtgg cgagggcgtg gctgcgacag
     5161 gcggcggagg gcggagccga cgcgttcgtc gcgggagagc tcgcggcgat cgaggcgcag
     5221 gcggccgccg aggcacgagc ggcgtggccc aaggcatgga aggcgctgtc ccgcaagcga
     5281 ctccgcttct gggcgtgagc cgacccgtcc gcgccgccgg aggcatcgtc ctgcggggcg
     5341 atggcggcga ccgatcggtg gccgtggtgc accgtccccg gtacgacgat tggtcgttcc
     5401 ccaagggcaa actcgaggac ggggaggacg aggcgacggc ggccctgcgc gaggtggagg
     5461 aggagaccgg cctgcggtgc cacctgggac cgagcgtcgg cgccgtgacc taccgggacc
     5521 gtcgaggccg gctcaaggtc gttcggtact tccagatgga tgcggacggc ggggggttcg
     5581 ctccgaaccg cgaggtggac gagcttcgct gggtcgccgt cgaggacgcg atgcggctgc
     5641 tgacctatcc gcacgaccgc aggatgttgc ggcgggtcgc gacgggcgcg ccggcctccg
     5701 cgtccgcgct ctacgtcgtt cgacacgcca aggccgggat ccgcgaggcg tggtccggtc
     5761 ccgacgagga gcggccgctc acccggcggg ggaggaagca ggcgcgccgc ctcgtcgggt
     5821 ggttccgcga cctggagatc gaccgcgtcc tgaccagccc gttcgtccgg tgcctccaga
     5881 ccgtggagcc gctcgccgac gctcgtgggc tcccggtcga ggtcgccacc gagttgcgtg
     5941 agggcgcgag cgtggacgag ttgctgcgtg cgctcgcgac gttcggcgac cgcccgacgg
     6001 tcgtctgcgg tcacggccgc gagatccggt cgatgatcga ccggatcgag gccggcggcg
     6061 cgacgatcga gggcgcccgc gggatcgcca agggttcggt ctgggtcctg gaccgggagg
     6121 gcgaacggat cgtagccgcg cactacctgc cggcgccgcc cggctagcac gtggcctccg
     6181 acacgcgctt cgccgtgctc gacctgggca gcaccacctt ccagctcctc gtcgcggatg
     6241 cggacacgca aggctcgctc acgccggtgt tccgcgaccg cgtcgtcctg aacctcgggc
     6301 gggtgctcgc cgggcggggc cggatccccg aggacgtagc cggccgcgcc gccgagaccg
     6361 ctcggcgtct cgccgacctc gccgtgcgga tgggggccga ccacgtcgtc cccctcgcca
     6421 ccagcgccct gcgggacagc cccgatcgcg acgcgctggc gtcgctgctc gagggagcgg
     6481 tcggaacgga ggtgcggttc atcgacgggc gcgaagaggc ccgcctcacc ttcgctggcg
     6541 tggcggcgag cgtcgcgctc ggccagggtc cgacgctgct cctcgatctg ggcgggggga
     6601 gcctcgaggt cgctctcgcg ggccccgacg gcctccgatg gggcaggagc ctcccgatcg
     6661 gcgccggtcg catgacgggc ctgctggtca cccgcgaccc gccgactcgg gacgaacgca
     6721 aggcggtgcg ggccgcggtc caggagaccg tgtctccgct cgtcgaggag gtcgcggcgg
     6781 ccgagccggc tcgctgcgtg gcgagcggcg gcacggccgg cgccctcgct cgggtcctcg
     6841 cggcgcgccg gtggggggcc ccgcccgctt cgctcaacca gtacgaggtc accgtgtccg
     6901 agctccgcga cctgacccgg gagctcgcct ccctgtcgct cgccgagcgc ctgcgcgtgc
     6961 ccggggtgga cgagcgtcgt gcggagctcc tgcccgccgg cggctggatc ctgacgagcg
     7021 ccgccgccga gctcggcgcg aagcgcctcg tgcactccga atggggcctg cgcgaggggg
     7081 cggtgctcga cgcgctgggg ctcgccgacc gcccgatccc gacgccgggc gagctccggc
     7141 gtcgatcggt cgagcggctg gtccgcgcat ggggtgagaa ccggcggcac gtggacgtgg
     7201 tcgcccggat cgcccagcgg ctgttcgacg acacccccga gctgcacggt ctgggggtgc
     7261 tcgagcgcga gctgctgggt cacgccgcgc ggctgcacga gatcggtgca cggatctcgc
     7321 cggcccgatt gcacaagcac ggtgcctacc tggtcgagaa cgccggcctg cgcgggttca
     7381 cccccgacga gatcgcgatg atcgcctccc tcgttcggtt ccaccgaggc aaggacccgc
     7441 gcccggtgta cccggcgttc gcggccctgt ccgacgcgga gcgcgaggcc gtcgtgctgc
     7501 tggtgggtct gctgcggatc gcgcacgcga tcggacgtgg gcccgagggt gaccggctcg
     7561 aggtcgcgac cgacccacgc gacggcacgg tcaacatcgt gatctccggc tcggacaacc
     7621 cggacgccgc ggtcgcggag gcccgctcgg cggcggccct gctcgcacgg acgctcgggg
     7681 gcccgatcga gatcggggtc ggcatcgccg gtcgtcgctg acgccgatca ccggcgcccg
     7741 atcacgcgtc cctgggcgcc agccagctcg cgagataccc gacgcgctcg aacccgatcc
     7801 ggtcgtagat ccgcaccgcg tcgccgtccg gctcggcgag caggtagacg cggtccgcgc
     7861 ccatctccgt ggcttccgag acgcaccgcc gcgtgagcat ctccgcgtgc ccgcgacgtc
     7921 gcgcgtaggg gaacgtgacg acgtgatcga cgaacccggt gcgctcgagc accaggagcg
     7981 ccgcgagggc gacccgccgg ccgccgtcga tcaccgagaa ccagcggcga ccggccggga
     8041 tcaggacgtc gcgctccatc gtctgcagct ggtcgagcgt gtccgcgtcc gagatgtcga
     8101 acagccgcgc cgagtcccgg tggtccgacc agaacggtcc gtcgaacgca gagacctcct
     8161 cgacctcgcc catgggctcg acctcgcgcc cggcggccac catcaccagg tcccagacga
     8221 tccgttcgcc gcgggtgctc gcggcgacga tcaggtccgt ctggtcctcg gggtggaaga
     8281 ccacgacgtg ggagcgctcg ctgccgctcg cgcgcatcgc gggcaggagc gatccctcga
     8341 tctcctcgag ccggaccggc tgccgggtct cgaccctcgc gtagttcggc tcctggatcg
     8401 ccgggaaccg agcgtccgag acgacggcgc cccaccatcg agcctccgtg cgctcgaaca
     8461 ggtcgtccag ggcccgccag aagcggagga gcggttcgag gtcggccacg cggccatctt
     8521 cgcagccgac gtccgcgccg agccgtcccg gacgcccgtg cgacgcaggg gctgcgatag
     8581 gctcgccccg ccgtgatcac ctttcgcgtc taccacgacg gctcgctcaa gcgcgagatg
     8641 ccgttcgaga ctcggaccat cgaggaggcc cgcgagggcg accagcgcgt gtggatcgac
     8701 gtcgtcgacc cgaccgacga cgagctcacc gagctgcagg ccaggctgga cctgcacgag
     8761 ctcgcgatcg acgacagccg ccactggggg cagcgcgcca aggtcgactt ctacccgcag
     8821 cacctgttcc tggtcgcgca cgggatcgac caggacgagc gcggcgagat cgtcgatcgg
     8881 gaggtgcacc tgttcgccgg ccagggcctc tacgtcgcca cggtgcgccg cgagccgagg
     8941 ttcgacttcg ccaaggtggc gacgcggctc gagagcgccc cggatctgtc caaggagggg
     9001 atcgggttcc tcctgtacct gatgctcgac gagatcgtcg acgggtacct cgacgtgatc
     9061 gagcggttcg aggacgcggc cgacgacatc gaggacgacg tggcgaccga ggaggagggc
     9121 gcggagggcg aggccacgcg gacgctcgcg cgacgcattt tcagccttcg ccaacaggtc
     9181 gttcggttcc ggcggctcgc gacgccgatg cgcgaggtgg tggacctcct gatcgagacg
     9241 ccggcgatcg tgacctccgc gctcgccccg tacttccgcg acgtcctgga tcacgtcatc
     9301 cggatgacgg agctgagcga caacgtccgt gacctgctca cctcggcgcg ggagctgcag
     9361 ctcgcccagg tggccaaccg gcagaacgtc gtgatgaagc agctcaccgc atgggccgcg
     9421 atcatcctga tcccgacgct gatcgcgggg atctacggga tgaacttccg acacatgccc
     9481 gagctcgagt gggcgttcgg ctacccgttc gcgctcgcgc tgatgggcgt ggccgccttc
     9541 ctgctgtacc ggagcttccg caagcgcgag tggctctgag atgtgccgag ccctcaaggt
     9601 cctgtgcgtg gccgcggatc gatccgcgct cgccgagctg aagcgagcgg cggtctcggc
     9661 cgagtgggag ctcgcgcccg gcgcgatgac cgaggatgat gcgatccgcc agctacatgg
     9721 cgatcgtccg cacgtcctcg tcgtgttcgg atcgttcccg accctggtcg agcgcgcgct
     9781 ggaggcctac ccggccctgc gcgtgatctc ggaccgcccg gtcccggggg cggtgacggt
     9841 cggctcgctc gagcaggtcc gttcggccgt caagccgtcg aaccgttccg gcggcccggt
     9901 ccgcgcctag cgccggcggc tttggctacg cacgctgacg ttcgaagagg aggcatacct
     9961 ctacgagcat ccggcctcag ggcgcacaga gatcgttgcg cactcacgac ggtcatcctg
    10021 ccaggttggc acgagccaac gacatgcccc ttgaggctcc agtctgttat ggcggctacc
    10081 atggggacgt gccgaccaag gtcctgctcg cggctccccg cggctactgc gcgggggtcg
    10141 agc
//
LOCUS       DATBRK010000192         5286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1040337, whole genome shotgun sequence.
ACCESSION   DATBRK010000192 DATBRK010000000
VERSION     DATBRK010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040337"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..643)
                     /locus_tag="VIH70_05780"
     CDS             complement(35..643)
                     /locus_tag="VIH70_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8115906.1"
                     /translation="MARATGMSELVTAERTKDAFRRLVDPELPGLYSLARRLVRDGAE
                     DLVQEALLQAYRAFGTLRDEAAAGRWLKTILVNVFRDDLRKRTRSVEEISVEEVEGFS
                     LYRTLIEVDPLPYSDALHHDFLTAFGKDDVREVLLRLPEIYRAPLVLKYMDGFVTKEI
                     ARMLDVPLGTVLARLHRGRKRFEQEMWAYAEEASLLAGRDDR"
     gene            758..1255
                     /locus_tag="VIH70_05785"
     CDS             758..1255
                     /locus_tag="VIH70_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115907.1"
                     /translation="MTTIAAERQETGGADTSLRVGSWLMGIAAVGFIGYAVIFFVRNF
                     TDSFLELGIGPNEVNVGKDEIQAFSPSLFEYVSHLHLAVSGFIAATGVAVLFLVAYGV
                     RRGQLWAWVGAVSAPVLGLAVALPAHYPNHFDTLGHLGLIYLAVLIFVAGALLALRGI
                     LARRG"
     gene            1275..1457
                     /locus_tag="VIH70_05790"
     CDS             1275..1457
                     /locus_tag="VIH70_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115908.1"
                     /translation="METETLLCAKCGGPADGWRCAICGSVANDHDERHLHVGSDRYCT
                     IRCAGCGEADVLCTCV"
     gene            complement(1470..1850)
                     /locus_tag="VIH70_05795"
     CDS             complement(1470..1850)
                     /locus_tag="VIH70_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8115909.1"
                     /translation="MRVTMDRSSPEPLWEQLSTVIAERIRRGSLAPGTRLPPVRALAG
                     DLELAPNTVAKAYRALEDQGLIVGRGRRGTFVTDRLPKRLTGRELRLGEAADAFVRRG
                     RQLGFGDDDIRRALIRTLRERLPS"
     gene            1899..2933
                     /locus_tag="VIH70_05800"
     CDS             1899..2933
                     /locus_tag="VIH70_05800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8115910.1"
                     /translation="MGVRVGIGLFTAQLPANSKRSFEQEYRETLELVRLAERVGFDSA
                     WVSEHHGSSDGYLPSLLPMLAAFAAVTERIELGTGVILTPLHDPLRLAEDAAVVDQLS
                     GGRLLLGIGNGWREEEFRMFEASRAERGARTEETIEVLRRAWTGRRFSFEGRTLRYDR
                     VKVTPQPARRGGPPILLGGYDRKAVIRAGRLADGYITDETGTDEIRSNLALLDREATG
                     VGRDPRTSSIVLLQNAFAWRDGDPWPLIREGVVQQLGTYEAWGNDADTPELDRLEPAA
                     PSEDDLRLSTPAGPPDDVTRALLATIRAAGDRDVHLVVRLHYPGMDLETASRAAELFA
                     AEVMPALKAG"
     gene            complement(2965..3312)
                     /locus_tag="VIH70_05805"
     CDS             complement(2965..3312)
                     /locus_tag="VIH70_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8115911.1"
                     /translation="MGAPVVHFEIYGKDASKLQSFYADLFGWEIHADNPMNYGIVHTN
                     SGDKGIGGGITEGDARVNVVVDVDDLQKYLDKAVSMGGEVVTPITEIPDMVTWAEFRD
                     PAGNVIGISLAGS"
     gene            complement(3348..3809)
                     /locus_tag="VIH70_05810"
     CDS             complement(3348..3809)
                     /locus_tag="VIH70_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8115912.1"
                     /translation="MAQVLRPDAGELEVRIQARPETVFEFFVDPEKMTRWKGTEAELD
                     PRPGGAYRVGGIAGGATVAGEFVEIDPPRRLVFTWGWEGDEEVPPGSSTVEVTLTPDG
                     DATVLHLVHRDLPAGQGPRHMEGWEHFVPRLVEAASGRDPGPDPWSMSGAM"
     gene            complement(3778..4113)
                     /locus_tag="VIH70_05815"
     CDS             complement(3778..4113)
                     /locus_tag="VIH70_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8115913.1"
                     /translation="MEAALKAIAEPRRRQILMLLRGGEMSAGDIASHFEVTRPAISQH
                     LAVLKEAGLVSERRDGTRRIYRFRPEGVEELRAFIDDMWTERLATLKDEAEREERRRR
                     GAGPATRRR"
     gene            complement(4345..>5286)
                     /locus_tag="VIH70_05820"
     CDS             complement(4345..>5286)
                     /locus_tag="VIH70_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115914.1"
                     /translation="SATVAVGSTATRSLLMMSRTVVMPPTKPSRRRLGREALRAWYVP
                     RRRAYPWRGSHDPYAVWVSEVMLQQTQASRVVPAFRSFLHRFPTVRALAAAPRRDVVL
                     EWGGLGYNRRAVGLSEAARAIVRDHGGRIPRDPATLRELPGIGPYTAAAVASLGFGQP
                     VAVVDTNVRRVVARVHVGIDGHEAPAKQVWALADAWLDRDDPATWNQAVMDLGREVCR
                     PEPRCDVCPLRRVCRFRRSGVTARRGPRRQGSFEGSSRQARGAVVDALCSKRSLTPAS
                     LAAEIGFARSRVDAAVESLVLDGLLEFYAGRVRLAGS"
BASE COUNT          804 a         1884 c         1801 g          797 t
ORIGIN      
        1 gcctccgagc acgggatcag tccctgcgtc tcggtcatcg atcgtccctc ccagcgagca
       61 ggctcgcttc ttctgcgtag gcccacatct cctgctcgaa ccgcttgcgc cctcggtgga
      121 gacgggcaag gacggtcccg agcggaacgt ccagcatgcg ggcgatctcc ttggtgacga
      181 acccgtccat gtacttgaga accaacggcg cccggtagat ctccggaagg cgcaggagga
      241 cctcgcgcac gtcgtccttc ccgaacgccg tgaggaagtc atggtgcagg gcgtccgagt
      301 agggaagggg gtccacctcg atcagggttc ggtacagcga gaacccctca acctcctcga
      361 cggagatctc ctccaccgaa cgggtacgct tgcggaggtc gtcgcggaag acgttgacga
      421 ggatcgtctt cagccaccgc ccggccgccg cttcgtccct gagcgttccg aacgcgcggt
      481 aggcctgcag caacgcctcc tgcacgagat cctccgctcc gtctcgaacc agccgccggg
      541 ccagcgaata caggcccggc agctcggggt cgaccagccg ccggaacgcg tccttcgtcc
      601 gctctgcggt cacgagctcg ctcatcccgg tcgctctggc caccaatacg cgcctccgac
      661 aacgaggacg acgaacgggc cgaccttatt gcatcgcacg gaagctgcaa tacggtgcag
      721 ccgccgctcg tcctggacca tcgaggaggt gatcgcgatg acgacgatcg cggcagagag
      781 gcaggagacc ggtggcgccg acacgtcgct ccgggtgggg tcgtggctga tggggatcgc
      841 ggccgtgggg ttcatcgggt acgcggtgat cttcttcgtc cggaacttca ccgacagctt
      901 cctggagctc gggatcgggc cgaacgaggt gaacgtgggc aaggacgaga tccaggcatt
      961 cagcccgtcg ctgttcgagt acgtgagcca ccttcacctg gcggtttcgg ggttcatcgc
     1021 ggcgacgggg gtcgcggtgt tgttcctcgt cgcgtacggg gtgcgtcggg ggcagctttg
     1081 ggcctgggtc ggcgccgtga gcgcgcccgt ccttgggctt gcggtggcgc tcccggccca
     1141 ctacccgaac cacttcgata cgctcgggca cctcgggctg atctatctgg ccgtgctgat
     1201 cttcgtggcg ggagcgctgc tcgcgctccg cgggatcttg gcgcgccggg gctagagggg
     1261 aggaccggag agcggtggag accgagaccc tcttgtgcgc gaagtgcggt ggtccggccg
     1321 acggctggag atgcgcgatc tgcggctcgg tggcgaacga tcacgacgaa cggcatctcc
     1381 acgtcggatc cgaccgctac tgcacgatca ggtgcgcggg gtgtggtgag gccgacgtgc
     1441 tctgcacctg cgtgtaggcg ttcggcgggt cagctcggca ggcgttcgcg gagcgttcgg
     1501 atcaacgctc gtcggatgtc gtcgtcgccg aacccgagct gcctgccgcg gcgaacgaac
     1561 gcgtccgcgg cttcgccgag ccggagctcc cgacctgtga gacgtttcgg caaccggtcc
     1621 gtgacgaacg tcccccgtcg cccgcgcccg acgatcaacc cttggtcctc gagcgcccgg
     1681 tacgccttcg cgacggtgtt cggcgcgagc tcgagatcac ccgctagcgc ccggacggga
     1741 gggagccggg tgccgggggc gagcgagcct cggcggatcc gttcggcgat caccgtggag
     1801 agctgctccc agagcggctc gggtgagctc cgatccatcg tgacccgcac gcgggcatca
     1861 tccccgaccg gtgcgagagg ccgtgtaacg tctcggcgat gggtgtgcgc gtcggcatcg
     1921 ggctgttcac ggcgcagctc ccggctaact cgaagcgctc cttcgagcag gagtaccggg
     1981 agacgctcga gctcgtgcgc ctggccgaac gcgtcgggtt cgactcggcg tgggtgagcg
     2041 agcaccacgg ttcctcggac ggatacctgc cgtcgctgct cccgatgctg gccgcgttcg
     2101 ccgccgtgac cgaacggatc gagctcggta caggcgtcat cctcacgccg ctgcacgacc
     2161 cgctccggct ggccgaggac gcagcggtcg tcgaccagct ctcgggaggc cgcctgctcc
     2221 tggggatcgg gaacggctgg cgcgaggagg agttccggat gttcgaggcg tcgagggccg
     2281 aacgcggcgc acggaccgag gagacgatcg aggtgctccg ccgggcgtgg acgggtcggc
     2341 ggttctcgtt cgagggacgc acgctccggt acgaccgcgt gaaggtcacc ccgcaacccg
     2401 cgaggcgggg cggcccgccg atcctgctgg gaggctacga ccgcaaggcg gtgatccgag
     2461 ccggacgtct cgccgacggc tacatcaccg acgagaccgg aaccgacgag atccggagca
     2521 acctcgccct cctcgaccgt gaagcgaccg gcgtcggacg tgacccgcga acgtcgtcga
     2581 tcgtgctcct gcagaacgcc ttcgcctggc gcgacggcga cccgtggccg ctcatccgcg
     2641 agggcgtcgt gcagcagctg gggacctacg aggcctgggg gaacgacgcc gacacgccgg
     2701 agctcgaccg cctcgagccg gcggccccgt ccgaggatga ccttcggctc tcgacgccgg
     2761 cgggaccgcc cgacgacgtg acccgcgcgt tgctcgcgac gatccgggca gccggcgatc
     2821 gtgatgtcca cctggtcgtg cggctgcact acccggggat ggatctcgag accgcctcgc
     2881 gggcggccga actcttcgcc gcagaggtca tgcccgccct gaaggccggc tgaccactcg
     2941 gcttcgtcga tcgcgcctac ccgatcagga gccggcgagc gagatcccga tcacgttgcc
     3001 ggcggggtcg cggaactccg cccacgtcac catgtccggg atctcggtga tcggtgtcac
     3061 gacctcgccg cccatcgata ccgccttgtc caggtacttc tggaggtcgt ccacgtcgac
     3121 gacgacgttc acgcgcgcgt cgccctccgt gatgccaccg ccgatcccct tgtcaccgga
     3181 gttcgtgtgg acgatcccgt agttcatcgg gttgtccgcg tggatctccc acccgaacag
     3241 gtccgcgtag aacgactgca gcttggacgc gtccttcccg tagatctcga agtgcacgac
     3301 cggtgcgccc atcgactcct ccctccttcc gacggccgag acgaccgtca catcgctccc
     3361 gacatcgacc aggggtccgg ccccgggtcc cgtcccgacg ccgcctcgac gagccgcggg
     3421 acgaagtgct cccatccctc catgtgcctc ggcccctgac ccgcgggcag gtcccgatgc
     3481 accagatgca gcaccgtcgc atcgccgtcc ggcgtcagcg tgacctcgac cgtgctggag
     3541 ccgggcggga cctcctcgtc accctcccac ccccaggtga acaccaggcg acggggaggg
     3601 tcgatctcga cgaattcgcc ggccacggtc gcgccccccg cgatcccgcc cacccggtac
     3661 gcgccgccgg gacgcgggtc gagctcggcc tcggtgccct tccagcgcgt catcttctcg
     3721 gggtcgacga agaactcgaa cacggtctcg ggacgcgcct ggatgcggac ctcgagctca
     3781 ccggcgtcgg gtcgcaggac ctgcgccacg ccgcctcctt tcctcgcgct cggcctcgtc
     3841 cttcagcgtg gcgagccgct ccgtccacat gtcgtcgatg aacgcccgga gctcctcgac
     3901 cccctcgggc cggaagcggt agatccgcct cgtgccgtcg cgacgttcgg acacgaggcc
     3961 ggcctccttg agcacggcga ggtgctggct gatcgccgga cgcgtgacct cgaagtgcga
     4021 ggcgatgtcg ccggccgaca tctcgccacc gcggaggagc atgaggatct gccggcggcg
     4081 cggttccgcg atggccttga gcgcggcctc catgccgagc agggtaggtt cgacttacgt
     4141 tagcgtcaac ttacaggcac ccctgccgga gtgtgcgatt cgctttccgc cgaccgtcag
     4201 gcttcataca gacagaaccc tgactgcgtg ggcgctcgac acggcgtcgc cgtcagggat
     4261 ctgacagaca caaccctgac cgtgccaccg ggcgacgcac cccaaccaag gaccgcggcc
     4321 gaatcgaggt gcgtgtgacg ccggtcacga gcccgccagg cggacccgcc cggcatagaa
     4381 ctcgagtaac ccgtcgagca cgagagactc gaccgccgcg tccacgcgac ttctcgcgaa
     4441 cccgatctcg gcggccaggc tggcgggcgt cagggaccgc ttcgagcaca acgcatccac
     4501 gaccgcacct cgggcctgcc ggctcgagcc ctcgaaggac ccctgccgtc tcggtccccg
     4561 ccgagcggtc acgcctgaac gccggaaccg gcaaacccga cggagcgggc agacgtcgca
     4621 tcgaggttcc ggtcgacaca cctcccgtcc gagatccatc accgcctggt tccaggtggc
     4681 ggggtcgtcg cgatcgagcc acgcgtcggc gagcgcccag acctgcttcg ccggcgcctc
     4741 gtgaccgtcg atcccgacgt gcaccctggc caccacacgc cgcacgttgg tatcgacgac
     4801 cgcgaccggt tggccgaacc cgagcgaggc gaccgccgcc gccgtgtacg gaccgatccc
     4861 ggggagctcg cgcagcgtcg ccgggtcgcg cgggatccgg ccgccgtggt cgcggacgat
     4921 cgcgcgggcg gcctccgaga gcccgaccgc gcgacggttg tagcccagac cgccccattc
     4981 caggacgacg tccctgcggg gtgcggccgc gagcgcgcgg accgtgggaa agcggtgcag
     5041 gaacgagcgg aacgccggca ccacgcggct cgcctgcgtc tgctggagca tcacctcgga
     5101 cacccagacc gcgtacgggt cgtgcgaacc gcgccacggg tacgcgcgac gccggggcac
     5161 gtaccacgcg cgcagggctt ccctgccgag gcgccggcga ctaggcttgg tgggcggcat
     5221 gacgacggtc cgggacatca tgagcaagga cctggtcgcg gtcgaaccca ccgccacggt
     5281 cgccga
//
LOCUS       DATBRK010000193         7193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2342479, whole genome shotgun sequence.
ACCESSION   DATBRK010000193 DATBRK010000000
VERSION     DATBRK010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342479"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VIH70_05825"
     CDS             <1..472
                     /locus_tag="VIH70_05825"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEY8115915.1"
                     /translation="RVGAGPFPTEDHGPGGDRLGERGQEFGTVTGRKRRCGWFDAVIL
                     RYAARVNGLTELFVTKLDVLSGFETVPVCTAYRAEGETFDDVPPHQSLFHKAEPVYEE
                     LEGWGEEIGAATAFGDLPAAARKYLERIQELVGVQVGVVSVGPGREQSLTSGVV"
     gene            513..1793
                     /gene="purD"
                     /locus_tag="VIH70_05830"
     CDS             513..1793
                     /gene="purD"
                     /locus_tag="VIH70_05830"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004728326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY8115916.1"
                     /translation="MRVLVVGAGGREHALAWRLARDPAVERVLAAPGNAGIAREATCF
                     GVAADDVDGLLALVERERVDLVVVGPEGPLVAGLADRLAAAGHPVFGPSSRAARLEGS
                     KGWAKDLCERYGIPAAGALSAATMTEGLDALDEFGPPYVVKVDGLAAGKGVVITEERD
                     EAVRALELALVWGEFGDAGSTVVVEEHLIGREVSAFALSDGATALPLGFAQDFKRVDD
                     DDAGPNTGGMGAYSPVPFVDEAIAERIRTEILEHTVRALAEEGIPYQGVLYAGLMLTD
                     QGPRVLEFNCRFGDPETQVLMPRLRSEPAELLLACARGDLGNAEADLRPGACVTVVLA
                     SGGYPGAYETGFVIEGLDAAEGVEGATVFHSGTAKRQGRVVTAGGRVLSVSATGASIA
                     EARDRAYEASSCLSFEGMRHRTDIAELAAKEERT"
     gene            1790..2263
                     /locus_tag="VIH70_05835"
     CDS             1790..2263
                     /locus_tag="VIH70_05835"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEY8115917.1"
                     /translation="MSEPAPLVGVLVGSAAELPLMQQATAILEKLEIPHEIRVMSSSR
                     SPDLVDEYARTAFDRGLKALVCTTGISGHLAAAVAARTIVPVIGVPIATDQMGGEQAL
                     YVTAQMPSGVPVAAVGVDSAMNAAVLAAEIVGVADPDVQKRLWRFKDDLAEGLRL"
     gene            2352..3659
                     /gene="purB"
                     /locus_tag="VIH70_05840"
     CDS             2352..3659
                     /gene="purB"
                     /locus_tag="VIH70_05840"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY8115918.1"
                     /translation="MIPRYVLPEMAAVWSEEARLGHWLEIEILAAEALAGLGIVPDAD
                     AREIRASASFDAERVAEVERVTRHDVAAFVDVVAASAGTAGRWVHFGLTSSDVLDTGL
                     ALQLRDASDLLLERLERMLAVAKRMALANRSVVMASRTHGVVAEPTSFGHKVALWAFE
                     LDRDRDRLLRAREAVSVGAISGAVGTYAQVDPRVEEEVCAKLGLRAAEASSQIVARDR
                     HAELMGALAITASTLDAIATEIRHLARTEVREVQEPFATGQKGSSAMPHKRNPVRCER
                     VTGLARVIRGNLQAALENTVLWHERDISHSSAERVILPDSTIALHFMLSEMLAVLEGL
                     EVYPERMRENLEVGGGLAFSQNVLLALVDAGLARDEAYAIVQTAAAAAWDEGASFRDL
                     LAADARVRERIGGRLGELFDPVAALRNLDVVFDRLEKVEVREG"
     gene            3656..4612
                     /locus_tag="VIH70_05845"
     CDS             3656..4612
                     /locus_tag="VIH70_05845"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY8115919.1"
                     /translation="MSGTAARLHGQGKVRDIYDVGDERVLLVATDRISAFDVVLPNPI
                     PDKGRVLTGLTLFWLERTTDVAPNHLASADRRDFPEPFRSEPALAGRAMLARRAEVIP
                     VECVVRGYLTGSGLKQYRAEGRVCGVALPGGLDESSRLPAPIFTPTTKAAAGHDLPLT
                     FDETVDVVGRGLAERLREISIALYERGAEIAAERGILLADTKFEFGFADGELMLIDEV
                     LTPDSSRFWPADGYRAGRAQPSFDKQFVRDWLDASGWDHEPPAPDLPPEVVERTSLKY
                     REAYERVAASPFDHYRHLSGVTSGGDAETDAAALERLRREHA"
     gene            4609..4869
                     /gene="purS"
                     /locus_tag="VIH70_05850"
     CDS             4609..4869
                     /gene="purS"
                     /locus_tag="VIH70_05850"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY8115920.1"
                     /translation="MNVRFEVLVQLKPGLLDPQGKAVEGSLPALGWTNVSGVRVGRHV
                     ELTIEADSEEAAWSQVEAMAARLLANPVIEEFQILRAGQVVR"
     gene            4877..5611
                     /gene="purQ"
                     /locus_tag="VIH70_05855"
     CDS             4877..5611
                     /gene="purQ"
                     /locus_tag="VIH70_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY8115921.1"
                     /translation="MSEDRRPRIGVVTFPGSLDDRDALRAVRVMGGEGVGLWHADRDL
                     RSVDAVILPGGFSYGDYLRCGAIARFAPIMDEVRGFAEAGGAVVGICNGFQILCEAGL
                     LPGALIRNRQLRFVCRWVNVRVETSVSPLSADLVAGEILRVPVKHGEGQFVASAGDLD
                     RIERDGLVVFRYCSPEGATEGPTGDPHNPNGASNSIAGLRNEAGNVVGLMPHPEHAVD
                     PDVGETGGQPMFASLLSSTLARVSAA"
     gene            5608..>7193
                     /gene="purL"
                     /locus_tag="VIH70_05860"
     CDS             5608..>7193
                     /gene="purL"
                     /locus_tag="VIH70_05860"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY8115922.1"
                     /translation="MSASEPLHRQLGLTDEELASIRSTLGRDPNRDELAMYAVMWSEH
                     CSYKSSKVHLRALPSEGRSVLVGPGQDAGAVDVGDGIAVVFKLESHSHPSAVEPYQGA
                     ATGVGGIVRDVLSMGARPIALLDPLRFGPLDDERNRWLFEGVVAGIGGYGNCIGVPTV
                     GGEVQFGPAHTANPTVNVMCVGVAPAERLVTSDREVHEGSLLVLFGAATGRDGIGGVS
                     VLASSTLEEGAGERRPSVQIGDPFAEKLLIEASLELVERDLLEGLQDLGGAGITCAVS
                     ESASRAGLGAELDLGAVPLREPDLEAFEILTSESQERMLAIVHADRLGDVGDVCERWG
                     LSTAVIGRLVRGGGLRATFRGDAVVDVPARSLANGPVYTRPMAAPDEVEDAIAEDPTF
                     ATVTTPPDEAFLAVLGSPNVASKRWVWEQYDSIVQGGTVMPPGADAALVRIEGTLKAL
                     ALSTDGKGRFGALDPYLGAAHSVAEAARNVACAGATPLAITNCLNFGNPERPEVMWQF
                     AQSVRGIADACRAFETPVTGG"
BASE COUNT          974 a         2448 c         2736 g         1035 t
ORIGIN      
        1 ccgcgtgggc gccggcccgt tcccgaccga ggaccacgga cccggcggtg accggctggg
       61 cgaacgtggg caggagttcg ggacggtgac cggtcgcaag cgccgctgcg ggtggttcga
      121 cgccgtgatc ctcaggtacg cagcccgcgt gaacgggctg accgagctgt tcgtcaccaa
      181 gctcgacgtg ctctcggggt tcgagaccgt cccggtgtgc acggcgtacc gggccgaggg
      241 cgagacgttc gacgacgtcc ccccgcatca gtcgctgttc cacaaggcgg agccggtcta
      301 cgaggagctc gagggctggg gcgaggagat cggcgcggcc accgcgttcg gcgatctgcc
      361 cgcggcggcc cgcaagtacc tggagcggat ccaggagctc gtcggtgtgc aggtcggcgt
      421 ggtctcggtc ggtccgggtc gcgaacagag cctgacctct ggcgtcgtgt gagcggatca
      481 cggtgggacg atcgtcccgc catgcttgcg cgatgagggt cctggtcgtc ggtgcggggg
      541 ggcgcgagca cgcgctcgcg tggcggctcg cacgagaccc ggcggtcgaa cgcgtcctgg
      601 ccgccccggg gaacgccggg atcgcgcggg aggcgacgtg cttcggcgtc gccgcggacg
      661 acgtcgacgg cctgctcgcg ctcgtcgaac gcgaacgcgt cgacctcgtg gtcgtgggcc
      721 ccgagggtcc gctcgtcgcc ggtctcgccg accggctcgc ggccgcgggg catcctgtgt
      781 tcggaccctc gagccgggcc gcgcggctcg agggctccaa ggggtgggcg aaggacctgt
      841 gcgagcgtta cgggatcccg gcggcggggg ccctgagcgc cgcgacgatg accgaggggc
      901 tcgacgcgct cgacgagttc gggcccccat acgtggtgaa ggtcgacggg ctcgccgcgg
      961 gcaagggcgt cgtgatcacc gaggagcgtg acgaggccgt ccgggcgctc gagctcgccc
     1021 tcgtgtgggg cgagttcggc gacgcggggt cgaccgtggt cgtcgaggag cacctgatcg
     1081 gccgggaggt ctcggcgttc gcgctctccg acggggcgac cgccctgcca ctcgggttcg
     1141 cgcaggattt caagcgcgtc gatgacgacg acgccggacc caacaccggg gggatgggcg
     1201 cgtactcccc cgttccgttc gtcgacgagg cgatcgccga gcgcatccgg accgagatcc
     1261 tcgagcacac cgtccgcgcg ctggcggagg agggcatccc gtaccagggc gtcctgtacg
     1321 cgggtctgat gctcacggac cagggtccga gggtgctcga gttcaactgc cgcttcggcg
     1381 acccggagac ccaggtcctg atgccgcggc tccggtccga acccgccgag ctcctcctcg
     1441 cgtgcgcccg gggcgacctc gggaacgccg aggcggacct gcggcccggc gcctgcgtga
     1501 cggtcgtcct ggcctccggc ggctaccccg gtgcgtacga gaccgggttc gtgatcgagg
     1561 gtctcgacgc ggccgagggc gtggagggcg cgacggtgtt ccattcgggc acggcgaagc
     1621 ggcagggtag agtggtcacc gcgggagggc gcgtgctgtc ggtgagcgcg accggcgcct
     1681 cgatcgccga ggccagggac cgcgcatacg aggcgagctc gtgcctctcg ttcgagggga
     1741 tgcgtcaccg cacggacatc gcggagttgg cggcgaagga ggagcggaca tgagcgaacc
     1801 ggcaccgctg gtcggcgtgc tggtcggttc ggcggccgag ctcccgctca tgcagcaggc
     1861 caccgcgatc ctggagaagc tcgagatccc gcacgagatc cgcgtgatgt catcatcgcg
     1921 cagccccgac ctcgtcgacg agtacgcgcg aaccgcgttc gaccgcggat tgaaggccct
     1981 ggtgtgcacg acggggatct ccgggcacct cgccgcggcg gtcgcggccc gaacgatcgt
     2041 gccggtaatc ggcgttccga tcgcgaccga ccagatgggt ggcgaacaag ccctgtacgt
     2101 gacggcgcag atgccgagcg gcgtgccggt ggccgcggtc ggcgtcgact cggcgatgaa
     2161 cgccgccgtc ctggccgccg agatcgtcgg cgtcgccgac cccgacgtgc agaagcggct
     2221 ctggcggttc aaggacgacc tggcggaggg cctccggctg tgagcggacg ctcgaccgtc
     2281 cggcctgacg gggggcggcc attcgcgagt tcgagctcgc gtcaggatca ccgcgagccc
     2341 cacgccgcgc catgatcccg cggtacgtcc tcccggagat ggccgccgtc tggtccgaag
     2401 aggcgcggct cggacactgg ctcgagatcg agatcctcgc cgccgaggcc ctggccggac
     2461 tcggcatcgt cccggatgcc gacgcgcgcg agatccgagc gtcggcgtcg ttcgacgccg
     2521 agcgcgtggc cgaggtcgag cgcgtcacca ggcacgacgt ggccgcgttc gtggacgtcg
     2581 tcgccgcgtc cgcaggtacg gcgggccgct gggtgcactt cgggctgacg tcctcggacg
     2641 tcctggacac cggactcgcg ctgcagctgc gcgacgcgag cgacctgctg ctcgaacggc
     2701 tcgaacggat gctcgccgtc gccaagcgga tggcgctcgc caaccggagc gtcgtgatgg
     2761 ccagccggac gcacggcgtc gtcgcggagc cgaccagctt tggtcacaag gtggcgctct
     2821 gggccttcga gctcgaccgc gaccgcgacc ggttgctgcg tgcgcgcgag gccgtgagcg
     2881 tcggcgcgat cagcggggcc gtcggaacct atgcgcaggt cgatccgcgc gtcgaggagg
     2941 aggtgtgcgc caagctcggc ctgcgtgccg ccgaggcctc gagccagatc gtggcgcggg
     3001 accggcacgc ggagctcatg ggcgcgctcg cgatcaccgc ctcgacgctc gacgcgatcg
     3061 cgaccgagat ccgccacctc gcccgcaccg aggtccgcga ggtgcaggag ccgttcgcca
     3121 cggggcagaa gggttcgagc gcgatgcccc acaagcggaa ccccgtccgc tgcgagcgcg
     3181 tgacggggct cgcccgggtg atccgcggga acctgcaggc ggcgctcgag aacaccgtgc
     3241 tctggcacga gcgagacatc tcgcattcgt cggccgagcg ggtgatcctg cccgactcga
     3301 cgatcgcgct gcacttcatg ctctcggaga tgctcgcggt gctcgagggg ctcgaggtgt
     3361 accccgagcg gatgcgggag aacctggagg tcggcggcgg gttggcgttc tcgcagaacg
     3421 tgttgctcgc gctggtggac gcgggcctgg ctcgcgacga ggcgtacgcg atcgtgcaga
     3481 cggcggcggc cgcggcgtgg gacgagggcg cgtcgttccg ggacctgctc gccgcggacg
     3541 ctcgcgtgcg cgaacggatc ggcggcaggc tcggcgagct gttcgacccg gtggcggcgc
     3601 tgcggaacct cgacgtcgtc ttcgaccgac tcgagaaggt cgaggtccgc gagggatgag
     3661 cggcacggcg gcccggctgc acggacaggg aaaggttcgc gacatctacg acgtcggcga
     3721 cgaacgggtc ctgctcgtgg ccaccgatcg catcagcgcg ttcgacgtgg tcctgcccaa
     3781 cccgatcccc gacaagggcc gcgtcctgac cggactcacg ctgttctggc tcgaacgcac
     3841 gacggacgtc gcccccaacc acctcgcgag cgccgaccgc cgcgacttcc ccgagccttt
     3901 ccgcagcgag ccggcgctcg ccggccgggc gatgctcgcc aggagggcgg aggtgatccc
     3961 cgtcgagtgc gtggtgcgcg gctacctcac gggttcgggg ctgaagcagt accgggccga
     4021 gggtcgggtg tgcggtgtcg cgcttcccgg gggcctggac gagtcatcga ggctccccgc
     4081 gccgatcttc acgccgacga cgaaggccgc cgccgggcac gatctgccgc tcacgttcga
     4141 cgagacggtg gacgtggtgg gccgcgggct cgcggagcgt ctgcgcgaga tctcgatcgc
     4201 cctgtacgag cggggggcgg agatcgccgc ggagcgcggg atcctcctcg ccgacacgaa
     4261 gttcgagttc gggttcgccg acggcgagct gatgctgatc gacgaggtgt tgaccccgga
     4321 ctcgtccagg ttctggcctg cggacggcta ccgcgccggg cgcgcccagc cgtcgttcga
     4381 caaacagttc gtccgagact ggctggacgc gagcggctgg gatcacgaac cccccgcgcc
     4441 cgacctgccc cccgaggtcg tggaacgcac ctcgctcaag taccgagagg cctacgagcg
     4501 ggtcgccgcc tcgccgttcg atcactaccg gcatctgagc ggtgtgacct ccggcggcga
     4561 cgccgagacc gatgcggccg cgctcgagcg cctccgacgg gagcacgcgt gaacgtgcgg
     4621 ttcgaggtcc tggtccagct caaaccgggc ctgctcgatc cccagggcaa ggcggtcgag
     4681 ggatcgcttc cggccttggg gtggacgaac gtgagcggcg tgcgcgtcgg ccggcatgtc
     4741 gagctcacga tcgaggccga cagcgaggag gccgcgtggt cgcaggtcga ggcgatggcc
     4801 gcccggctcc tggcgaaccc ggtgatcgag gagttccaga tcctgcgggc cgggcaggtt
     4861 gttcgttgag cggcacatga gcgaagaccg tcggccgagg atcggcgtcg tcacgttccc
     4921 cgggtcgctg gacgaccggg acgcgctccg cgcggtgcgc gtcatgggcg gggaaggcgt
     4981 cgggctttgg cacgcggatc gcgatctccg tagcgtcgac gcggtgatcc ttcccggcgg
     5041 gttctcctac ggcgactacc ttcggtgcgg cgcgatcgct cggttcgcgc cgatcatgga
     5101 cgaggtgcga gggttcgccg aggccggcgg cgccgtcgtc gggatctgca acgggttcca
     5161 gatcctgtgc gaggccgggc tgctgcctgg cgcgctgatc cggaatcggc agctccgctt
     5221 cgtgtgccga tgggtgaacg tccgcgtcga gacctccgtg tcgccgctgt cggcggacct
     5281 ggtcgcgggc gagatcctgc gggtcccggt caagcacggt gaggggcagt tcgtcgcgtc
     5341 cgccggcgat ctcgaccgaa tcgagcgcga cgggctggtc gtcttccgat actgctcgcc
     5401 agagggcgcg acagagggcc cgacaggcga tccgcacaac cccaacggcg cctcgaactc
     5461 gatcgcgggg ctccgcaacg aggcgggcaa cgtcgtcggc ctgatgccgc accccgagca
     5521 cgcggtcgac cccgacgtcg gcgagacggg cggacagccg atgttcgcct cgttgctgtc
     5581 gtccacgctc gcccgcgtct ccgccgcgtg agcgcgtccg agccgcttca ccggcagctc
     5641 ggactcaccg acgaggagct ggcgtcgatc cgatcgaccc tcggccgcga tcccaaccgc
     5701 gacgagctcg cgatgtacgc cgtgatgtgg tccgagcact gctcctacaa gtcctcgaag
     5761 gtccacctgc gcgccctgcc cagcgagggt cgctcggtgc tcgtcggccc gggtcaggac
     5821 gccggcgcgg tcgacgtggg cgatgggatc gcggtcgtgt tcaagctcga gtcgcactcg
     5881 cacccctcgg cggtcgagcc ctaccagggc gcggcgaccg gggtgggcgg gatcgtgcgc
     5941 gacgtgctct cgatgggggc ccgacccatc gcgttgctgg acccgctgcg gttcggcccg
     6001 ctcgacgacg agcggaaccg gtggttgttc gagggcgtgg tggccgggat cggcggctac
     6061 ggaaactgca tcggcgtccc gacggtgggc ggcgaggtgc agttcgggcc ggcccacacg
     6121 gcgaacccca ccgtgaacgt gatgtgcgtc ggcgtcgcgc ccgccgagcg gctcgtcacg
     6181 tcggaccggg aggtccacga gggctcgttg ctcgtcctgt tcggcgcggc caccggtcgc
     6241 gacggcatcg gcggggtgtc ggtgctcgcg tccagcaccc tcgaggaggg tgccggggaa
     6301 cgccgaccga gcgtgcagat cggcgacccg ttcgccgaga agctcctcat cgaggcctcg
     6361 ctcgagctcg tcgaacgcga cctgctcgag gggctccagg acctgggcgg ggcggggatc
     6421 acgtgcgccg tgagcgagtc ggcgtcccgg gccgggctcg gcgccgagct cgaccttggc
     6481 gcggtcccgc tccgcgagcc cgacctggag gcgttcgaga tcctgacgag cgagtcgcag
     6541 gagcgcatgc tcgcgatcgt gcacgccgac cggctcggcg acgtcgggga cgtgtgcgag
     6601 cggtgggggc tctcgaccgc ggtgatcggc cggctcgtcc gaggcggcgg cctgcgcgcg
     6661 accttccgtg gcgacgcggt cgtcgacgtc ccggcccggt ccctcgcgaa cggacccgtc
     6721 tacacgcgcc cgatggctgc acccgacgag gtcgaggacg cgatcgccga ggacccgacg
     6781 ttcgcgaccg tgaccacgcc gcccgatgag gcgttcctcg cggtcctcgg ctcgccgaac
     6841 gtcgcgagca aacgctgggt gtgggagcag tacgactcga tcgtccaggg cggaacggtg
     6901 atgccgcccg gcgcggacgc ggcgctcgtg cggatcgagg gcaccctgaa ggcgctcgcg
     6961 ttgtcgacgg acggcaaggg ccggttcggc gcgctcgatc cctacctcgg cgcggcgcac
     7021 tcggtcgccg aggctgcgcg caacgtcgcc tgcgccggcg cgacgccgct cgcgatcacg
     7081 aactgcctga acttcggcaa tcccgagcgg ccggaggtga tgtggcagtt cgcgcagtcg
     7141 gtgcgaggga tcgcggacgc ctgccgcgcg ttcgagaccc cggtgaccgg cgg
//
LOCUS       DATBRK010000194         6260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1905476, whole genome shotgun sequence.
ACCESSION   DATBRK010000194 DATBRK010000000
VERSION     DATBRK010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905476"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2430
                     /gene="uvrA"
                     /locus_tag="VIH70_05865"
     CDS             <1..2430
                     /gene="uvrA"
                     /locus_tag="VIH70_05865"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8115923.1"
                     /translation="EGTRFQVLAPVVRGRKGEYEKLLKDLAGQGFVRARIDGEVRELA
                     EQIRLPRNYKHTIEVVVDRLVAKPDIRRRVADSVESALQLTEGLVAIAVQTHDGEDVQ
                     TYSQALACTFDGLSFDELQPRNFSFNSPYGACQTCDGLGIRLEVDAALVVPDPDLSIA
                     DGALAPWSTGRIEYWDRVLDAVADSHGFSTSTPWKKLPKKVRDVVLYGSDEEIYVKYK
                     NRYGRTRSYWTTYEGVVPVVERRHVETDSESQRERLEEFMREVPCRSCGGARLRPETL
                     AVTVGDLNISQLTSLSIRDTLSFTREARLSEREQMIAERLMKEIRERLQFLVDVGLDY
                     LTLSRASGTLAGGEAQRIRLATQIGSGLVGVLYILDEPSIGLHQRDNRRLIDTLVRLR
                     DLGNTLVVVEHDEATIAASDHVVDIGPGAGNAGGEIVYTGDLKGLLECDASLTGAFLS
                     GRRVIPTPQVRRAPGSRRLRVLGAREHNLKDIDLEIPLGLLVCVTGVSGSGKSTLVQE
                     VLLRALMQKVYRSRALPGKYRRLVGWEQIDKVIDIDQSPIGRTPRSNPATYTGMFDQI
                     RKLFSQVPEARVRGYQPGRFSFNVRGGRCENCAGDGQIKIEMHFLPDVYVTCEVCKGH
                     RYNRETLEVRYKHRSIADVLEMSVDEALEFFQNIPVIKRHLQTLSDVGLGYVKLGQPA
                     PTLSGGEAQRIKLSSELQKRATGSTLYILDEPTTGLHFEDIRKLLSVLQRLVAQGNTV
                     IVIEHNLDVVKTADWIVDLGPEGGDAGGEVVVTGTPETVAAHPDSHTGRFLAEVLGTL
                     PLEPVGASREA"
     gene            2489..4459
                     /locus_tag="VIH70_05870"
     CDS             2489..4459
                     /locus_tag="VIH70_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8115924.1"
                     /translation="MPQALRWKLPPLAFVLALGAAACAEPPTATVNLGSGVRFLPEVA
                     DSLNDAGRYPSIVTNTDGLPVVAYFGFEDKLEPGEVPVTRPVGSPSIPGVLLATVSEQ
                     GYWTRGAIAIAQEIPNVPIAFDPAFEPSIADLTADKVTGLAMVADGDTYHAVWGSDAG
                     VYYATGSLDPATTTQAVVTRVTKAPGYGLSIGMAGGSPAIAFYTSTSSDAKVHLATPN
                     GDSWSDDMIAEGAGCETCRTAVIGGDRPTVAYSKGGAGVEVASNDGENGFVSFEVSAT
                     GGQGLSGVAMDGGIALSYYDAANGQVTIATRTGDGSVDTVAAAEVPEGSAEGPGAATG
                     IALGSDGSIDVTWWDADAGIRFAAGDPGSLKAVDTGTSTVDGSFPSITVNEDGSVTYL
                     AWYATTESDLLVGGYGAFEDVPFAEPSPTPTGPISTPTGPTAQCTPVANGSVTVVAQG
                     IAFTEGDCIQATAGEAFKIEFDNRDEATQHNIVVFAGEEATGDTVFRGELITGPDQAT
                     YDVPALDAGTHAFHCEVHPTTMIGSVEVAEGGGAGGGGGGGQGGGGQGGGGGGAANAT
                     VTASGIAFDTSTIDLPANQPSTIHFVNEDNATQHNIAIFPSADDLANPLFRGELLTGP
                     GEIDYAVDPLKAGEYYFHCDVHPTMNGTVNVS"
     gene            4528..>6260
                     /gene="uvrC"
                     /locus_tag="VIH70_05875"
     CDS             4528..>6260
                     /gene="uvrC"
                     /locus_tag="VIH70_05875"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY8115925.1"
                     /translation="MSFPRPDASSIPDGPGAYLFRDEDGRVVYVGKARSLRKRLANYW
                     GRDLHPRTEAMVAAAASVEWIMASTEVDALLLEYNLIQEHRPRFNVRYRDDKSYPYLA
                     LTVGERWPRAQVLRGAKRKNVRYFGPFGHAYAIRETLDALTRVFPVRTCSNSFFDQRA
                     RARRPCLYYDIGRCSGPCVPEQTGVTEESYRADVEALGEFLSGHERPIMERLEREMEE
                     AARRREYELAAKHRDQLTAARRALESQEMVLSLPEDIDVVGLAEDDLEAGFQVFFVRR
                     GRVMGRKGWVVDRVEDLDRPGLVGSFLRELYMQRQDVPPRILVPELPADADVLGEWLS
                     ARREGPVRFAVPERGTKRKLMETVTQNATDQFRRHKLRRASDFGARSRALAELGRRLE
                     LEQPPLRIECYDISNLGPTDTVGSMVVFEDGLPKRSDYRRFEIKGVPGQDDFASMEEM
                     LRRRLARLAKDREPGERRRFAYPPALIVVDGGRGQLSSATKALADADVHIPAIGLAKR
                     LEEVYFPDRPDPLVIPRGSEALFVLQHLRDEAHRFAVTYHREKRGKRALASPLDDVAG
                     VGPSRKKALLKR"
BASE COUNT          985 a         2148 c         2229 g          898 t
ORIGIN      
        1 gaggggacga ggttccaggt cctcgcgccg gtcgtccggg ggcgcaaggg cgagtacgag
       61 aagctgctga aggatctcgc gggtcagggg ttcgtacgcg ccaggatcga cggtgaggtc
      121 cgcgagctcg ccgagcagat ccggctgccc cgcaactaca agcacacgat cgaggtcgtc
      181 gtcgaccggc tcgtcgccaa gccggacatc cggcgccggg tcgcggactc ggtcgagtcc
      241 gcgctgcaac tcaccgaggg gctcgtcgcg atcgccgtgc agacccacga cggcgaggac
      301 gtccagacgt acagccaggc gctcgcgtgc acgttcgacg ggctctcgtt cgacgagtta
      361 caaccccgga acttctcgtt caactcgccg tacggcgcct gtcagacctg cgacggcctc
      421 ggcatccgcc tcgaggtcga cgcggcgctc gtcgtgcccg accccgacct gtcgatcgcc
      481 gacggggcgc tcgcgccgtg gtcgaccggc cggatcgagt actgggaccg ggtcctggat
      541 gccgtcgcgg actcccacgg cttctcgacg agcaccccct ggaagaagct ccctaagaag
      601 gtccgcgacg tcgtgctcta cggctcggac gaggagatct acgtcaagta caagaaccgg
      661 tacgggcgca cccgctcgta ctggaccacg tacgagggcg tggtgccggt ggtcgagcga
      721 cggcatgtgg agaccgactc ggagagccag cgcgagcggc tcgaggagtt catgcgcgag
      781 gtcccgtgcc ggtcgtgcgg cggcgcccgg cttcgcccgg agacgctggc cgtcaccgtg
      841 ggcgatctca acatctccca gctcacgagc ctgtcgatcc gggacacgct gtccttcacg
      901 cgcgaggccc ggctcagcga gcgcgagcag atgatcgccg agcgactgat gaaggagatc
      961 cgcgagcgcc tgcagttcct cgtcgacgtg gggctcgact acctgacgct ctcgcgggcg
     1021 tcggggacgc tcgcgggggg cgaggcgcag cggatccgcc tggccacgca gatcggcagc
     1081 gggttggtcg gcgtgctcta catcctggac gagccctcga tcggcctgca ccagcgcgac
     1141 aaccgacgcc tgatcgacac gctcgtccgg ttgcgagacc tcgggaacac gctcgtggtg
     1201 gtcgagcacg acgaggccac gatcgccgcc tcggaccacg tggtcgacat cgggcccggc
     1261 gccggcaacg ccgggggcga gatcgtgtac acgggcgacc tgaaggggtt actcgaatgc
     1321 gacgcgtcgc tgacgggcgc gttcctctcg ggccgcaggg tgatcccgac accgcaggtc
     1381 cggcgcgcgc ctgggagccg acggctgagg gtgctcggcg cgcgcgagca caacctcaag
     1441 gacatcgacc tcgagatccc gctcgggctc ctcgtctgcg tgacaggggt gagcggctcg
     1501 ggcaagtcca cgctcgttca ggaggtcctg ctccgagcgc tgatgcagaa ggtgtatcgc
     1561 tcccgagccc tgccgggcaa gtaccggcgg ctcgtggggt gggagcagat cgacaaggtg
     1621 atcgacatcg accagtcgcc gatcggacgc accccgcgct cgaacccggc gacctacacc
     1681 gggatgttcg accagatccg caagctgttc agccaggtgc cggaggcgcg cgttcgcggc
     1741 taccagcccg gccggttctc cttcaacgtg cggggcgggc gctgcgagaa ctgcgcgggt
     1801 gacgggcaga tcaagatcga gatgcacttc ctgccggacg tctacgtcac gtgcgaggtg
     1861 tgcaaggggc accggtacaa ccgggagacg ctcgaggttc ggtacaagca ccgatcgatc
     1921 gccgacgtgc tggagatgag cgtggacgag gcgctcgagt tcttccagaa catcccggtg
     1981 atcaagcgac acctccagac cctgtccgac gtgggtctgg gctacgtgaa gctcgggcag
     2041 ccggcgccca cgctctcggg cggcgaggcg cagcggatca aactctcgtc cgagctgcag
     2101 aagcgcgcga ccggaagcac cctgtacatc ctggacgaac cgacgacggg tctgcacttc
     2161 gaggacatcc gcaagctcct gtccgttctg caacggctgg tcgcccaggg caacacggtg
     2221 atcgtgatcg agcacaacct ggacgtcgtg aagacggccg attggatcgt cgatctcggt
     2281 cccgagggtg gagacgcggg cggcgaggtc gtcgtgaccg gcacccccga gacggttgcg
     2341 gcgcacccgg actcgcacac ggggcggttc ctcgcggagg tgctcggcac gctcccgctc
     2401 gaaccggtgg gcgcgtcgcg agaggcctga cgccgtcgga cggatgctcc gcccggcgcc
     2461 cgtcaccttg ctaggattgc gcccgctcat gccccaggcc cttcgatgga agctgccgcc
     2521 gctggccttc gtgctcgccc tgggcgccgc ggcgtgcgcc gaacctccga ccgcgaccgt
     2581 gaacctcggg tccggggtcc gcttcctccc ggaggtcgcc gactcgctga atgacgccgg
     2641 ccgctacccc tcgatcgtga cgaataccga cggcctgcca gtcgtcgcgt acttcgggtt
     2701 cgaggacaaa ctcgagccgg gcgaggtgcc ggtcacgcgt ccggtgggtt ccccgtcgat
     2761 ccccggggtg ctcctcgcga cggtaagcga gcaggggtat tggacgcggg gagcgatcgc
     2821 catcgcgcag gagatcccca acgtgccgat cgcgttcgat ccggctttcg agccgtcgat
     2881 cgccgacctg accgccgaca aggtcaccgg gctcgcgatg gtcgccgacg gcgacacgta
     2941 ccacgcggtc tggggttcgg acgcgggtgt gtactacgcg accggctcgc tcgaccccgc
     3001 caccacgacg caggcggtcg tcacgcgggt gacgaaggcg cccggctacg gcctgtcgat
     3061 cggtatggcc ggcggcagcc cggcgatcgc cttctacacg tcgacctcgt ccgatgccaa
     3121 ggtgcacctc gcgaccccga acggcgactc gtggtcggac gacatgatcg ccgagggcgc
     3181 gggctgcgag acgtgccgga ccgcggtgat cggaggcgac cggccgacgg tcgcctattc
     3241 gaagggcggc gcaggcgtcg aggtagcgtc caacgacggg gagaacgggt tcgtgagctt
     3301 cgaggtctcg gccaccggcg gccaggggct gtcgggggtc gcgatggacg gcgggatcgc
     3361 gctctcgtac tacgacgccg cgaacggtca ggtgacgatc gccacccgca ccggtgacgg
     3421 atcggtcgac acggtcgcgg cggccgaggt cccggagggc agcgccgagg gacccggggc
     3481 ggcgaccggg atcgcgctcg gctccgacgg ctccatcgac gtgacctggt gggacgccga
     3541 cgcgggcatc cggttcgcgg ccggcgaccc cggttcgctc aaggcggtcg acacggggac
     3601 ctcgacggtg gacgggtcgt tcccgtcgat caccgtgaac gaggacggct cggtgaccta
     3661 cctcgcgtgg tacgccacca cggagtcgga cctgctcgtc ggcgggtacg gcgccttcga
     3721 ggatgtgccg ttcgccgagc ccagcccaac cccgacgggc ccgatcagca cgccgacggg
     3781 cccgaccgcc caatgcaccc cggtcgcgaa cggctccgtc acggtggtcg cgcaggggat
     3841 cgcgttcacc gagggcgact gcatccaggc caccgccggc gaggcgttca agatcgagtt
     3901 cgacaaccgg gacgaggcga cgcagcacaa catcgtggtc ttcgccggcg aggaggccac
     3961 gggcgacacc gtcttccggg gcgagctcat cacgggtcca gaccaggcga cctacgatgt
     4021 gccggcgctc gacgcgggca cccacgcctt ccactgcgag gtccacccga cgacgatgat
     4081 cggctcggtc gaggtcgccg agggcggcgg cgcaggcggc ggcgggggtg gtggccaggg
     4141 cggtggcggc cagggcggag gcggaggcgg cgccgcgaac gcgaccgtga cggcctccgg
     4201 gatcgcgttc gacacctcca cgatcgacct gccggcgaac caaccgagca cgatccactt
     4261 cgtcaacgag gacaacgcga cccagcacaa catcgcgatc ttccccagcg ccgatgacct
     4321 cgcgaacccc ctgttccgcg gcgaactcct gaccggcccc ggcgagatcg actacgcggt
     4381 ggacccgctc aaggcggggg agtactactt ccactgtgac gtccatccga cgatgaacgg
     4441 aacggtgaac gtctcctaag caaccgtaag gcgtccggga ggccgtccaa ccgggtttcc
     4501 gcaggttgcc gcgttacgat gggtcgcgtg agtttcccgc gacccgatgc atcgtcgatc
     4561 cccgacggcc cgggcgcgta cctgttccgg gacgaggacg gccgggtcgt ctacgtcggc
     4621 aaggccaggt ccctgcggaa gcggctggcc aactactggg gacgggacct ccatccccgg
     4681 accgaggcca tggttgccgc cgccgcctcg gtcgaatgga tcatggcctc caccgaggtc
     4741 gacgcgctcc tgctcgagta caacctgatc caagagcacc gaccccgctt caacgtgcgc
     4801 taccgagatg acaagtccta tccgtacctc gcgctgacgg tgggggagcg ctggccgcgt
     4861 gcccaggtcc tgcggggcgc caagcgcaag aacgtccggt acttcgggcc gttcgggcac
     4921 gcctacgcca tccgcgagac cctcgacgcc ctcacgcgcg tctttcccgt tcggacgtgc
     4981 tcgaactcct tcttcgacca gcgcgcccgg gcccgccggc cgtgcctgta ctacgacatc
     5041 ggccgctgct ccgggccgtg cgtcccggag cagacggggg tcaccgagga gtcgtaccgg
     5101 gcagacgtcg aggcgctcgg cgagttcctg tcgggtcacg agcgaccgat catggagcgg
     5161 ctcgaacgcg agatggagga ggcggcccga cgccgagagt acgagctcgc ggcgaagcat
     5221 cgcgatcagc tgacggcggc gcgccgcgcg ctcgagagcc aggagatggt gctgtcgctg
     5281 cccgaggaca tcgacgtcgt cggcctcgcc gaggacgacc tggaggcggg gttccaggtg
     5341 ttcttcgtcc gccggggtcg cgtgatgggc cgcaagggct gggtcgtcga ccgggtcgag
     5401 gatctcgacc ggccggggct cgtgggttcg ttccttcgcg agctctacat gcagcggcag
     5461 gatgtgcctc cgcggatcct ggtccccgag ctcccggccg acgccgacgt gctcggcgag
     5521 tggctctcgg cccggcggga gggtccggtg aggttcgccg tccccgagcg ggggaccaag
     5581 cgcaagctga tggagaccgt gacgcagaac gcgaccgatc agttccgccg ccacaagctt
     5641 cggcgggcct cggacttcgg agcccgctcc cgcgccctgg cggagctggg gcgccggctc
     5701 gagctcgagc agccgccact gcggatcgag tgctacgaca tctcgaacct cggcccgacc
     5761 gacacggtgg gttcgatggt cgtgttcgag gacggcctgc ccaagcggag cgattaccgg
     5821 cgcttcgaga tcaagggggt gcccggacag gacgatttcg caagcatgga ggagatgctc
     5881 cgccgcaggc tcgcgcgcct cgccaaggac cgggagcccg gcgaacgccg ccgcttcgcc
     5941 tacccgccgg cgctgatcgt ggtcgacggc ggtcgcgggc agctctcgag cgcgaccaag
     6001 gcgctcgccg acgccgacgt ccacatcccg gcgatcgggc tcgcgaagcg gctcgaggag
     6061 gtctacttcc ccgaccggcc ggacccgctc gtgatcccgc gtggctccga ggccctgttc
     6121 gtgctgcagc acctgcggga cgaggctcac cggttcgccg tgacctacca ccgcgagaag
     6181 cgggggaagc gcgcgctggc ctcgccgctc gacgacgtgg ccggggtggg tccgtcccgg
     6241 aagaaggcgc tgctgaagcg
//
LOCUS       DATBRK010000195         4337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1905609, whole genome shotgun sequence.
ACCESSION   DATBRK010000195 DATBRK010000000
VERSION     DATBRK010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905609"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VIH70_05880"
     CDS             complement(<1..550)
                     /locus_tag="VIH70_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8115926.1"
                     /translation="MIALETLPDERGRFGAYGGRYVPEILIPALDELAAAWSSLRDDP
                     AFRGELDALLRDFVGRPSAMTFAPRLSEATGYRIYLKREDLNHTGAHKINNTLGQVLL
                     ARRLGKGRIIAETGAGMHGVATATACALFGLPCVVYMGEEDTIRQAPNVARMQLLGAE
                     VVTVTSGSRTLKDALNEALRDWA"
     gene            complement(547..1317)
                     /locus_tag="VIH70_05885"
     CDS             complement(547..1317)
                     /locus_tag="VIH70_05885"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY8115927.1"
                     /translation="MGFLTDLVGDLRRRLERDPLDESGLLAVAMHLPPPRPFEDALRG
                     TRSALIAEYKRASPSAGAIAEPDAASQARAYEEGGAAAISVLTEPTRFDGALADLRAV
                     RLAVGLPVMRKDFLIHPAQVIESRAAGSDAVLLIAAALSELELKGMLAVAADLGLGTL
                     VETHSEEDLAKALATDAPVIGVNARDLETLEVDVERALAMLPEVPWDRVAVLESGVST
                     RDDVARAIDAGAGAVLVGETLMRSPDPGATIRSLRGAA"
     gene            complement(1360..2073)
                     /locus_tag="VIH70_05890"
     CDS             complement(1360..2073)
                     /locus_tag="VIH70_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY8115928.1"
                     /translation="MHLEVIPALDLAGGRLVNVLGTGQVAATAFGADPLTAATAFLDS
                     GATRLHVVDVDLARSGEVGNADVLGAVCGLGVPVQASGGVRTEEQVDALLARGAERVV
                     LGSAALHDRDGAGALVRRFGDRLVVGIEADGHTVRPRGTDLELPLRDLLVWLTGLEVR
                     RYLFTDVGRSGGLEGPDLDSIGAFAESSPVPVVVAGGIRGVDDLRALAALGGRIEGAV
                     IGRALYEGLDLHAAIAAVA"
     gene            complement(2085..>4337)
                     /gene="dnaE"
                     /locus_tag="VIH70_05895"
     CDS             complement(2085..>4337)
                     /gene="dnaE"
                     /locus_tag="VIH70_05895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY8115929.1"
                     /translation="QIITFQTIKGKQGIRDAARVLGFPASVGDRLCKMYPAAIMGRDE
                     PIEKALRLSHELRDAYEREPEAREIVDTARALEGLRREDSVHAAGVVIGDAPLVTYLP
                     LKLSKDSRDDSRRIVTQFDMHGVEDLGLLKMDFLGLRTLSVIEDTLSHLRRRDVELDI
                     DHVPLDDAAVYEMLCRADTTGVFQLEGGGMRSLIRQLAPDRFEDLMALVALYRPGPLS
                     AGMHVEYAERKHGRRPVSFPHADLEEILAPTYGIMVYQEQVMQIAVRMAGYSMAEADM
                     LRQAMGKKIRAKLMVHREKFIQGCVDNGYPDRLGTDLFDLMEPFADYGFNASHACAYG
                     YIAYQTAFLKAHHPVEYMSAILTSVRDDKDRKPFYLNACRLMGLEVLPPDVNESEQDF
                     APVTSGEPKIRYGLSAVRNVGSGAVQQIIEARRTKGSFTGYADYCRKVEPSVLTKKVL
                     ESLIYAGAFDSFGYRRRGLLENQDKISIPISAERKAEAAGQFSLFAGDEHATNEIDEG
                     VITSDEYDRRTLLRLEKEVLGQFVTDHPLLGVKERLAALADMEIAETEGLGDGDLVTV
                     AGIVASVSRRYTKKGEPYAQFRLEDLAGGVTVVVFPGQYESLQHLLEHDSILLVKGRA
                     DRRGRGEELQLRAVEVSEPDLGDGGRPTPPAGRLVIDLTARSCTGAVIATLKELLSAH
                     KGSSPVQVRFHSSQGVRPFDVGTFRVEPGAGLLSELRVLLGTEAVRLASLEPDDVVVL
                     PASGVPAATR"
BASE COUNT          642 a         1587 c         1496 g          612 t
ORIGIN      
        1 ccgcccaatc ccgcagcgcc tcgttgagcg cgtccttgag cgttcggctg ccggaggtga
       61 ccgtgacgac ctcggcgccg agcagctgca tccgggcgac gttgggcgcc tgccggatcg
      121 tgtcctcctc gcccatgtac acgacgcacg gcagcccgaa gagggcgcac gccgtggccg
      181 tcgccacccc gtgcatcccg gcccccgttt cggcgatgat ccgccccttg cccagccggc
      241 gggcgagcaa cacctggccc agggtgttgt tgatcttgtg cgccccggtg tggttcaggt
      301 cctcgcgctt gaggtagatg cggtacccgg tcgcctccga cagccgcggc gcgaaggtca
      361 tcgccgaggg ccggccgacg aagtcccgga gcagcgcgtc gagctcaccg cggaaggccg
      421 gatcgtctcg gaggctcgac caggccgccg cgagctcgtc gagcgcgggg atcaggatct
      481 ccggtacgta ccggcccccg tacgcgccga agcgcccccg ttcgtcggga agcgtctcca
      541 gcgcgatcac gccgcacccc gcagagatcg gatcgtggcc ccggggtcgg gagaccgcat
      601 cagcgtctct ccgaccagca cggctccggc gccggcgtcg atcgcgcgcg cgacgtcatc
      661 gcgggtcgag accccgctct cgagcaccgc gacgcggtcc cacggcacct ccggcagcat
      721 cgcgagggcg cgttcgacgt cgacctcgag cgtctccagg tcccgagcgt tcacgccgat
      781 gaccggcgca tcggtcgcga gcgccttggc gagatcctcc tccgaatggg tctcgaccag
      841 ggtgccgagc ccgaggtcgg cggcgaccgc cagcatcccc ttcagctcga gctccgagag
      901 ggccgcggcg atcaacagga cggcgtcgct gccggcggcc cgagactcga tgacctgtgc
      961 ggggtggatc aggaagtcct tccgcatcac cggaaggccc accgcgagcc gaaccgcgcg
     1021 gaggtccgcg agcgcgccgt cgaaccgcgt gggctcggtc agcaccgaga tcgccgccgc
     1081 tcccccttcc tcgtaggcac gcgcctggct cgcggcgtcc ggttcggcga tcgccccggc
     1141 cgagggggac gcccgcttgt actcggcgat cagcgccgac cgtgtccctc gcaaggcgtc
     1201 ctcgaacggt cgtggcgggg ggaggtgcat cgcgacggcc agcaagcccg actcgtcgag
     1261 cggatcgcgt tcgaggcggc gccggaggtc gccgacgagg tcggtcagga agcccatgga
     1321 gccgcaagtc tacgcacgcc aacgccgccg cccgcgacgt caggcgaccg cggcgatcgc
     1381 ggcgtgcagg tcgaggccct cgtacaacgc ccggccgatc accgcgccct cgatccggcc
     1441 gcccagggcg gccagggccc gaaggtcgtc gacgcccctg atcccgcccg cgacgacgac
     1501 cggcaccggc gaggactccg cgaacgcccc gatcgaatcg agatccggac cctcgagccc
     1561 gccggaacgc cccacgtcgg tgaacaggta cctgcggacc tcgagaccgg tcagccagac
     1621 gaggagatcc cggagcggca gctcgaggtc ggtgccccgc ggccgcacgg tgtggccgtc
     1681 cgcctcgatc cccacgacga gccggtctcc gaaccgccgg acgagcgcgc cggccccgtc
     1741 gcgatcgtgc aacgcggccg agccgagcac gacccgctcg gccccccgcg cgagcagcgc
     1801 gtcgacctgc tcctcggtgc ggacgccgcc gctcgcctgc acgggcaccc ccaggccgca
     1861 cacggcaccg aggacgtcgg cgttgccgac ctcgcccgag cgcgcgagat cgacgtcgac
     1921 gacgtgcagc cgcgtggcgc ccgagtcgag gaacgccgtg gcggccgtca atgggtcggc
     1981 gccgaacgcc gtcgccgcga cctggccggt ccccaggacg ttgacgaggc gaccgccggc
     2041 gaggtcgagc gccgggatca cctcgaggtg catgggccag aacgctaccg ggtggccgcg
     2101 ggaactccgg acgccgggag gacgaccacg tcgtccggtt cgagcgacgc cagacgcacg
     2161 gcctcggtgc cgagcaggac ccgaagctcg gagagcagcc cggcgccggg ttcgacgcgg
     2221 aaggtgccca cgtcgaacgg ccgcacgcct tgggacgaat ggaaccgcac ctggaccggc
     2281 gacgagccct tgtgagctga gagcagctcc ttgagggtcg cgatcaccgc gccggtacag
     2341 ctccgcgcgg tcaggtcgat caccaggcgg ccggccgggg gggtcggacg cccgccgtcg
     2401 cccagatcgg gttcggagac ctcgaccgcg cgcagttgca gctcctcgcc gcgaccgcgt
     2461 cggtcggcgc gacccttcac caacaggatc gagtcgtgct cgagtaggtg ctggaggctc
     2521 tcgtactggc cggggaacac gaccaccgtg acgccgccgg ccaggtcctc gagccggaac
     2581 tgcgcgtagg gctccccctt cttcgtgtag cggcgcgaga ccgaggcgac gatccccgcg
     2641 accgtgacga gatcgccgtc gcccagaccc tcggtctcgg cgatctccat gtcggcgagc
     2701 gcggcgagcc gctccttcac gcccaggagc gggtggtcgg tcacgaactg gccgagcacc
     2761 tccttctcca ggcggaggag ggtccgccgg tcgtactcat cggacgtgat cacgccctcg
     2821 tcgatctcgt tcgtcgcgtg ctcgtcaccc gcgaacagcg agaactgccc cgcggcctcg
     2881 gccttgcgct cggccgagat cgggatcgag atcttgtcct ggttctcgag gagcccgcgt
     2941 cgccggtacc cgaacgaatc gaacgcgccc gcgtagatca ggctctcgag caccttcttg
     3001 gtcaggaccg agggttccac cttgcggcag tagtccgcgt agccggtgaa cgaacccttc
     3061 gtccgccggg cctcgatgat ctgctgcacc gcgcccgagc ccacgttgcg gacggcggag
     3121 agcccgtatc ggatcttggg ctcgccgctc gtcaccggcg cgaagtcctg ttccgactcg
     3181 ttcacgtcgg ggggcagaac ctcgagcccc atcaggcggc acgcgttcag gtagaagggc
     3241 ttgcggtcct tgtcgtcgcg gacgctcgtc aggatcgccg acatgtactc gacggggtga
     3301 tgcgccttca ggaacgccgt ctggtacgcg atgtacccgt acgcgcacgc gtgcgacgcg
     3361 ttgaacccgt aatcggcgaa cggctccatc aggtcgaaca ggtccgtacc gagccggtcc
     3421 gggtacccgt tgtcgacgca gccctggatg aacttctcgc ggtgcaccat cagcttggcg
     3481 cggatcttct tgcccatcgc ctggcggagc atgtcggcct cggccatcga gtacccggcc
     3541 atccgcaccg cgatctgcat cacctgctcc tggtagacca tgatcccgta ggtgggggcg
     3601 aggatctcct cgagatccgc gtgcgggaac gacacgggcc ggcgcccgtg cttgcgttcg
     3661 gcgtactcga cgtgcatccc ggcgctgagc ggcccgggcc ggtacagcgc gaccagcgcc
     3721 atcaggtcct cgaagcggtc gggcgccagc tgccggatca gcgagcgcat cccgccgccc
     3781 tcgagctgga agaccccggt ggtgtcggcg cggcacagca tctcgtacac cgccgcgtcg
     3841 tccaggggca cgtgatcgat atcgagctcg acgtccctgc gccgcaggtg cgacagcgtg
     3901 tcctcgatga ccgagagcgt ccgcaggcca aggaagtcca tcttgagcag gccgaggtcc
     3961 tcgacgccgt gcatgtcgaa ctgcgtgacg atccggcgcg agtcgtcgcg ggaatccttg
     4021 gagagcttga gtggcaggta ggtcacgagc ggtgcatcgc cgatcacgac gccggccgcg
     4081 tgcacggaat cctcccgccg gagcccctcc agcgcccgtg ccgtgtccac gatctcgcgg
     4141 gcctcgggct cccgctcgta ggcgtcgcgg agctcgtggg agagccgcag cgccttctcg
     4201 atcggctcgt cccgccccat gatcgcggcc gggtacatct tgcagaggcg atcgccgacg
     4261 ctcgcgggga acccgaggac gcgcgcggcg tcgcggatcc cctgcttccc cttgatcgtc
     4321 tggaacgtga tgatctg
//
LOCUS       DATBRK010000196         9965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_466445, whole genome shotgun sequence.
ACCESSION   DATBRK010000196 DATBRK010000000
VERSION     DATBRK010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466445"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VIH70_05900"
     CDS             <1..184
                     /locus_tag="VIH70_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115930.1"
                     /translation="LEVDRRVHAEAIPAAPQEQLALARRLGFEEYPRQTFLEEYRRIT
                     RRARRAMERVFHEEEG"
     gene            186..569
                     /locus_tag="VIH70_05905"
     CDS             186..569
                     /locus_tag="VIH70_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8115931.1"
                     /translation="MPRRLIASGSPFEPVIGFSRAVVEGHHVVVAGTAPVMPDGGDPP
                     ADAFGQARRVLEIIVVALAEAGAGPEHVVRTRTYLTDAGDWEEVGRAHGEVFADIRPA
                     STMIVVSAFLDPRWRVEMEADAILP"
     gene            670..1056
                     /gene="mscL"
                     /locus_tag="VIH70_05910"
     CDS             670..1056
                     /gene="mscL"
                     /locus_tag="VIH70_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY8115932.1"
                     /translation="MLKEFKAFILRGNLVEIAVAFIMALAFAAVVASFVTDIVTPVIA
                     AIVGQPDFGSLKVDVGESAISYGKFLNAVITFLIVGFVMFLIVKAYNRMSGPKAATTT
                     SCPFCKTDIALDATRCPNCTSELQPA"
     gene            complement(1058..1954)
                     /locus_tag="VIH70_05915"
     CDS             complement(1058..1954)
                     /locus_tag="VIH70_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015624684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY8115933.1"
                     /translation="MPTRLVPTERAIDLGQTLGPMLRGRGDPTMRTARGVAERASRTP
                     DGPVSVRFAETAGGIEVEGWGPGARWLLDRAAAWCGAEDDDAGFDPERGLVRDLWRRH
                     AGLRIPRTGLTTERLIPVILEQKVTGNEARRAYRRLVTALAEPAPGPLGLVLPPDPER
                     VAALPYYAFHPFGVERRRAEVLRHVCARAAWVDEAAALPIDAAEDRLATVQGIGPWTV
                     AEVARLAFGDADAVSVGDYHVPNIVAWALAREPRGSDERMLELLEPYRPHRGRVQRLL
                     EVSGIRAPAFGPRMAPRAIDRI"
     gene            complement(2029..3459)
                     /gene="glnA"
                     /locus_tag="VIH70_05920"
     CDS             complement(2029..3459)
                     /gene="glnA"
                     /locus_tag="VIH70_05920"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY8115934.1"
                     /translation="MTTPKEVLALARESGTEIVDLRFSDLPGLMQHFSIPLHELSEES
                     FEDGLGFDGSSIRGFQEIQESDMLLVPDPDTAYLDPFTQHPTLNLHCFVKDPVTGEMY
                     TRDPRYVARKAELYLRQTGIADTSFWGPECEFYIFDAVRFDQNQHEGYYHLDAIEGVW
                     NSGRERELGGEPNLGYKPRYKEGYFPLPPMDHYQDLRSEMVLNLEKVGLSIEVHHHEV
                     GTAGQAEIDMRYGTLLATADNVMKYKYVVKNTARQAGKTVTFMPKPLFQDNGSGMHTH
                     QSLWKDDDNLFWDEVGYAGISDTARWYIGGLIAHAPSLLAFTNPTTNSYRRLVPGYEA
                     PINLVYSQRNRSACVRIPQYFKNPGAKRLEFRTPDPSCNPYLAFAAMLMAGLDGVKSK
                     IEPPEPMDKDLYDLPPEEKASVKQVPGSLEQVLDSLEADHQWLLEGGVFTQDVVDTWI
                     EYKRLHELDAVRLRPHPYEFALYYDL"
     gene            complement(3589..3882)
                     /locus_tag="VIH70_05925"
     CDS             complement(3589..3882)
                     /locus_tag="VIH70_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115935.1"
                     /translation="MNATAELGQLLDSLGDAVERGADQTRAFLASPAGRRIRAVTASG
                     LLLATPMILRHPFFRTPLGRVIEIGGAAALIAKGAAIIRDWEPAPGGVATGSG"
     gene            complement(3933..4592)
                     /locus_tag="VIH70_05930"
     CDS             complement(3933..4592)
                     /locus_tag="VIH70_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005201523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8115936.1"
                     /translation="MIRVVMLTDRSPGEVLPAAPTLLLDLKVEHLSVAAVGDALALGT
                     DAVLVDAAENPGQGHVVLRALRERDPRIPILAVIERRDVERFHWEEVADEVLFPGAPE
                     GEIRLRFAITRRRTGAVEETTIRLGPIALDAGTYRVSIEGAPLDLTFKEFELLRYLAE
                     RRGRVFTRPALLREVWGYDFYGGTRTVDVHVRRLRAKLGPAHEGLIETVRGVGYRAAD
                     V"
     gene            4644..4907
                     /locus_tag="VIH70_05935"
     CDS             4644..4907
                     /locus_tag="VIH70_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY8115937.1"
                     /translation="MPRVTVRLFAALRELAGSSRVEAEGATVGEVVQALSARYGERFE
                     GIARMGSVVVGGERADPATPLTEGQEVALLPPVSGGYRRPARP"
     gene            4920..5840
                     /locus_tag="VIH70_05940"
     CDS             4920..5840
                     /locus_tag="VIH70_05940"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY8115938.1"
                     /translation="MSMRFQRLLLVVNPVARTMSKPVLAVIEKALSADFRLEVAETKE
                     RGHASELAAQAVDEGVDLVVVFSGDGTINEAVNALAGTESALGVLPGGATNILVRALG
                     LPLDPVEATGVLIARALDDQAFKLSLGVADGRYFAVNCGAGVDAAAMRRLDQRFPKTK
                     SKFDRAAFRAVARELLVSYAGRSADLEVRIDDGPPLPSLSAMVGRTDPYTYYRDRGIR
                     MTPEASLDSGLDVLSVRKLARRSVPRIAWQVFGSARHVHGRDIDYVHDARHVLVTSET
                     PFPVQVDGDSLGDHRRLEVDLAPEALWVVA"
     gene            complement(5837..6601)
                     /locus_tag="VIH70_05945"
     CDS             complement(5837..6601)
                     /locus_tag="VIH70_05945"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY8115939.1"
                     /translation="MPETLLSDEALRALNARLEPTHPREIVRWALEESGLREIALASA
                     FQAEGTCVMHMATQIRPDVPVLFLETGFHFEETLAFKRRLTELLDLNVVELTGAYTPT
                     TQREAFGPRLYERHPERCCDINKVRPMLEALRGREAWITAFRRDSSPTRATAPIVDRY
                     ELEPGSWIVKVNPVASWTRRDTWSYLREHELPHNPLYDLGYASIGCAPCTRLSLPGEH
                     EREGRWAGLSKWECGIQVRGGGSEEGASAARSAAKA"
     gene            6706..7875
                     /gene="sat"
                     /locus_tag="VIH70_05950"
     CDS             6706..7875
                     /gene="sat"
                     /locus_tag="VIH70_05950"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEY8115940.1"
                     /translation="MPDDTTIAPHGGDLVDLLVPEGRREATRAEADHLPKLVVNAREL
                     SDLEMLAVGALSPLRGFVGEADYLSVLETMHLSSGPAWAIPVTLSLTDDDVKRIGAGS
                     SVALLPWEGGAPIAIVDVAEVFKRDRQKEALAVYATEDLEHPGVQALHDAGDFCLAGT
                     VRALALPEHDDFLPYRLTPAQTRAAFAERGWRTVVGFQTRNPIHRAHEYLQKCALEIV
                     DGLLVHPLVGETKGDDVPADVRMRCYEALFEGYYPKDRAMVSVFPAAMRYAGPREAIW
                     HAICRKNYGCTHFIVGRDHAGVGSYYGTYDAQRIFEEFEPGELGITPLMFEHSFWCNA
                     CEGMASPKTCPHGEGSRVSLSGTKVREMLRAGERPPAEFSRPEVADILIEAMREG"
     gene            7998..8981
                     /locus_tag="VIH70_05955"
     CDS             7998..8981
                     /locus_tag="VIH70_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8115941.1"
                     /translation="MTLVAATLGVIPAFAGTQDRLDAAKAELAKVQAELDRATAHWHS
                     AVMRLEATQAGILDARVRIRRLEDRVAAVGARLNRRAVIAFTNGPASTIDLLLSSGSF
                     TDFSDRLEFLGSMAQGDADLVIEREVAQEELRRERDNLLVLADRQAAVAEELGQAQDV
                     IDAQFDRISDRVAELTEQYRQEIAASRELVILGQTPDPDGSIQVCPVAGPNSFIDTFG
                     WPRPGGRTHQGIDLIAPFGTPIVAAHPGSVSYSSSSLGGLQAYVYGSGGYSFYAHLSG
                     YSSASGSVGAGTVIGYVGSTGDAGSTNHLHFEWHPGGGAAVDPYLMLLAVC"
     gene            9044..9574
                     /locus_tag="VIH70_05960"
     CDS             9044..9574
                     /locus_tag="VIH70_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HEY8115942.1"
                     /translation="MSEQVGHRRIDRIRTPEFVEGLSGLSLEDLRARRDECYAEREYL
                     SLLRRLVQGRAEILRAEVERRGSGGKAGSLVDQLSTILSSEGQGPSRGEAVKVGLPEE
                     EMLLARRRVERLVADAEISDPGTLDDERLAAAVELLVGEEHEVSEARNDVLQVMDALQ
                     EELKRRYRDDPSLVLS"
BASE COUNT         1451 a         3439 c         3515 g         1560 t
ORIGIN      
        1 gctcgaggtc gaccgacgcg tccacgccga ggcgatcccc gcggcgccac aagagcagct
       61 cgcgctcgcg cgacggctcg ggttcgagga gtatccgcgg cagacgttcc tggaggaata
      121 ccggcggatc actcgccgag ccaggcgagc gatggagcgg gtcttccacg aggaggaggg
      181 atgacgtgcc gcgccggctg atcgcgtccg gctcaccgtt cgaacccgtg atcgggttct
      241 cgcgcgcggt ggtcgagggc catcacgtcg tcgtggccgg gaccgcgccg gtcatgccgg
      301 acgggggcga tccgcccgcg gatgctttcg gccaggcccg acgtgtgctc gagatcatcg
      361 tggtcgcgct cgccgaggcc ggtgcgggtc ccgagcacgt cgtgcggacg cggacctacc
      421 tgaccgacgc aggcgactgg gaggaggtcg ggcgggccca cggcgaggtg ttcgccgaca
      481 tccgtccggc gagcacgatg atcgtcgtga gcgcgttcct ggatccgcgt tggcgcgtcg
      541 agatggaggc cgacgcgatc ctgccctagc ggaggctcgg aaggccgagc agcacgcctt
      601 gcgtcgtcgc gaggtggctt gcgaccatcg gggaatcagg ggcaaccacg acgagaagga
      661 gcgggtccca tgctgaagga gttcaaggcg ttcatcctga ggggcaacct cgtcgagatc
      721 gcggttgcgt tcatcatggc gctggcgttc gccgcggtcg tcgcctcgtt cgtcaccgac
      781 atcgtcacgc ccgtcatcgc ggcgatcgtt ggccaaccag atttcggcag cctgaaggtc
      841 gacgtcgggg agagtgcgat ctcctacggc aagttcctga acgcggtgat cacgttcctg
      901 atcgtcgggt tcgtgatgtt cctgatcgtc aaggcctaca accggatgag cggccccaag
      961 gccgcgacga ccacgagctg cccgttctgc aagacggaca tcgccctcga cgccactcgc
     1021 tgcccgaact gcaccagcga gcttcagccc gcgtagctca gatccggtcg atcgcgcggg
     1081 gcgccatcct cggtccgaac gccggcgcgc ggatcccgct cacctccagg agccgctgca
     1141 cccggccgcg gtgcggacgg tagggttcga gcagctcgag catccgctcg tccgacccgc
     1201 gaggttcgcg ggcgagcgcc caggccacga tgttgggtac gtggtaatcg cccaccgaca
     1261 cggcgtccgc gtcgccgaac gcgagccggg ccacctcggc aaccgtccac ggtccgatcc
     1321 cctgcaccgt cgcgaggcga tcctcggcgg cgtcgatcgg gagcgcagcc gcctcatcga
     1381 cccatgccgc ccgcgcacac acatgccgga gcacctcggc acggcggcgc tcgacgccga
     1441 acggatggaa cgcgtagtag gggagcgcgg cgacgcgttc aggatccgga ggtagcacga
     1501 ggccgagcgg gccgggagca ggttcggcca gagcggtcac cagtcgccgg tacgcgcgcc
     1561 gagcctcgtt gccggtgacc ttctgctcga ggatcacggg gatcaggcgt tcggtggtca
     1621 gaccggtccg cgggatccgc aggcccgcat gacggcgcca gaggtcccgc accagccctc
     1681 gctcgggatc gaacccggcg tcgtcgtcct cggcaccgca ccaggcggca gcccggtcga
     1741 gcagccatct cgcgcccgga ccccatccct cgacctcgat cccgcccgcg gtctccgcga
     1801 accggacgga caccggtccg tcgggggtcc gcgacgcgcg ttcggcgacc ccgcgcgcgg
     1861 tccgcatcgt cggatcgccg cgcccgcgta gcatcggtcc gagcgtctgg cccagatcga
     1921 tcgcgcgctc ggtcgggacg agtcgggtcg gcacgacggg caggctacgc tctgcgacgc
     1981 acgcgtccgg ctcgaaagcc gggccggccg accgtcgacg cgggtgacct acagatcgta
     2041 gtagagcgcg aactcgtacg gatgtggccg tagccggacc gcgtcgagct cgtgcagtcg
     2101 cttgtactcg atccaggtgt cgaccacgtc ctgcgtgaag acgccgccct cgagcagcca
     2161 ctggtggtcg gcctcgaggg agtcgaggac ctgctcgagc gaaccgggga cctgcttgac
     2221 cgacgccttc tcctcgggcg gcaggtcgta caggtccttg tccatcggct cgggcggctc
     2281 gatcttgctc ttcacgccgt cgagtcccgc catgagcatc gccgcgaacg ccaggtacgg
     2341 gttgcacgag gggtcgggtg tccggaactc caggcgcttg gcgccggggt tcttgaagta
     2401 ctgcgggatc cgaacgcagg cgctccggtt ccgctgcgag tacaccaggt tgatcggggc
     2461 ctcgtagccc gggaccagcc ggcggtacga gttcgtcgtc gggttggtga aggccagcag
     2521 tgacggcgca tgtgcgatca gccctccgat gtaccaccgc gcggtgtccg agatgccggc
     2581 gtagccgacc tcgtcccaga acaggttgtc atcgtccttc cacaggctct ggtgcgtgtg
     2641 catccccgag ccgttgtctt ggaacagcgg cttcggcatg aacgtcacgg tcttgccggc
     2701 ctgccgcgcc gtgttcttga ccacgtactt gtacttcatg acgttgtcgg cggtcgcaag
     2761 cagcgtcccg tatcgcatgt cgatctcggc ctggcccgcc gtgccgacct cgtggtggtg
     2821 cacctcgatg ctcaggccga ccttctcgag gttcaggacc atctccgaac gcaggtcctg
     2881 gtagtgatcc atcgggggca gggggaagta gccctccttg taccggggct tgtagccgag
     2941 gttcggctcc ccgccgagct cgcgttcgcg tcccgagttc cagacgccct cgatggcgtc
     3001 gaggtggtag tagccctcgt gctggttctg gtcgaaccgg accgcgtcga agatgtagaa
     3061 ctcgcactcc gggccccaga acgacgtgtc cgcgatgccg gtctgtcgca ggtacagctc
     3121 cgccttgcgc gcgacgtagc gcgggtcgcg ggtgtacatc tcgcccgtga ccgggtcctt
     3181 gacgaagcag tgcaggttca gcgtcgggtg ctgcgtgaac gggtcgaggt acgcggtgtc
     3241 ggggtcgggg accaggagca tgtcggactc ctggatctcc tggaaccccc ggatcgacga
     3301 cccgtcgaac ccgagaccgt cttcgaagct ctcctcggag agctcgtgca gggggatgga
     3361 gaagtgctgc atgaggccgg gcaggtccga gaaacgcaga tccacgatct cggtgccgga
     3421 ctccctggcg agggccagga cctccttcgg cgtcgtcatc cttcgcgtct cccctctcgc
     3481 cggcgtgcga tggccaaggc tacgggcgcg cggccgccgc cgcggtctcc ggcgcgttaa
     3541 ctctgcgtta cgggatggga actgtcggct cggcggtctt tgcgcgagct aacccgaccc
     3601 ggtggcgacg ccgcccgggg ccggctccca gtcgcggatg atcgcggccc ccttggcgat
     3661 caacgccgcc gcgcccccga tctcgatcac ccggccgagc ggcgtccgga agaacgggtg
     3721 ccgcaggatc atcggggtag cgagcaggag cccgctcgcg gtcaccgcgc ggatgcggcg
     3781 cccggcgggc gacgccagga acgctcgggt ctggtcggcc ccgcgttcga cggcgtctcc
     3841 gagtgagtcg agcagctgac cgagctcggc ggttgcgttc acgaccggac cttacctccg
     3901 gggcctgtct atcaaacaat gaagccggag gcctagacat ccgcggcgcg gtacccgacg
     3961 ccgcgcacgg tctcgatcag gccctcgtgc gcaggcccga gcttcgcgcg gagccggcgg
     4021 acgtggacgt cgaccgtccg cgtcccgccg tagaagtcgt agccccagac ctctcggagc
     4081 agcgccggcc gggtgaagac ccgcccccgg cgctcggcta ggtaccgcag cagctcgaac
     4141 tccttgaacg tcagatccag cggcgcgccc tcgatgctca cccgatacgt gccggcgtcg
     4201 agcgcgatcg gcccgagccg aatcgtggtc tcctccaccg cccccgtccg ccgcctcgtg
     4261 atggcgaacc tcaggcggat ctcgccctcg ggggcacccg ggaacagcac ctcgtccgcg
     4321 acctcctccc agtggaagcg ctcgacgtcc cggcgttcga tcaccgcgag gatcgggatc
     4381 ctgggatcgc gttcgcggag cgcgcggagc acgacgtgcc cctggccggg gttctcggcc
     4441 gcatccacca gcaccgcgtc ggtgccgagc gcgagcgcat ccccgaccgc cgccaccgag
     4501 agatgctcga ccttgagatc gagcagcagc gtcggtgcgg cgggcaggac ctcccccggc
     4561 gagcgatcgg tgagcatcac gacgcggatc acggcccacc gagcgtacaa gcgggctcgc
     4621 cgcgacgata gccttgggtc gcgatgccac gcgtgaccgt tcggctgttc gcggcgctcc
     4681 gtgagctggc ggggtcctcc cgggtcgagg ccgagggggc caccgtcggg gaggtcgtcc
     4741 aggcgctctc cgcccgatac ggcgagcggt tcgaagggat cgccaggatg ggctcggtcg
     4801 tggtgggcgg cgaacgtgcc gaccccgcga cgcccctcac cgagggccag gaggtggcac
     4861 tgctgccccc ggtatctggg ggctatcggc gaccggcccg accctgagat acttccccca
     4921 tgtcgatgcg cttccagcgc ctcctgctcg tcgtcaatcc ggtggcccgc acgatgtcca
     4981 agcccgtcct ggccgtgatc gagaaggcgc tctcggccga cttccggctg gaggtcgccg
     5041 agaccaagga gcgcggccat gcctctgagc tcgcggcgca ggccgtggac gagggcgtcg
     5101 acctggtggt cgtgttctcg ggcgacggga cgatcaacga ggccgtcaac gcgctcgccg
     5161 gtaccgagag cgcgttgggc gtgctgccgg gtggtgcgac caacatcctg gtgcgggccc
     5221 tcggcctgcc gctcgacccg gtcgaggcga ccggcgtcct gatcgcgagg gcgctcgacg
     5281 accaggcgtt caagctctcg ctcggtgtgg cggacggccg gtacttcgcc gtcaactgtg
     5341 gggcgggcgt ggacgccgcc gcgatgcgcc ggctcgatca acggttcccc aagacaaagt
     5401 cgaagttcga ccgggccgcg ttccgcgccg tggcgcgaga gctgttggtg agctacgcgg
     5461 gccgttccgc ggacctggag gtgcggatcg acgacgggcc gccgctcccg tccttgtcgg
     5521 cgatggttgg acggaccgat ccgtacacgt actaccggga tcgagggatc cggatgacgc
     5581 cggaggcatc gctggactcc ggtctggacg tgctctcggt gcgtaagctg gcccgccgaa
     5641 gcgtcccgag gatcgcgtgg caggtgttcg gctcggcgcg ccacgtccac gggcgggaca
     5701 tcgactacgt gcatgacgcg cgccacgtgc tggtgacgtc ggagacgccg tttccggtcc
     5761 aggtcgacgg ggactcgctc ggcgaccacc ggcgcctgga ggtcgacctc gcacccgagg
     5821 ccctctgggt cgtggcttag gccttcgcgg ccgacctcgc cgcgctcgcg ccctcttcgg
     5881 acccgccgcc gcggacctgg atcccgcact cccacttcga caggccggcc cacctgccct
     5941 cgcgctcatg ttcgcccggg agcgagagcc gggtgcacgg cgcgcaaccg atcgacgcgt
     6001 acccgaggtc gtacagcggg ttgtggggga gctcgtgctc cctcaggtag gaccaggtgt
     6061 cccggcgcgt ccacgaggcg accgggttca ccttgacgat ccagctgccc ggttcgagct
     6121 cgtaccggtc gacgatcggg gccgtggccc gggtcgggga cgagtcgcgt cggaaagcgg
     6181 tgatccatgc ctcgcggccg cgaagtgcct cgagcatcgg ccgcaccttg ttgatgtcgc
     6241 agcatcgctc tggatggcgc tcgtacaggc ggggaccgaa ggcctcgcgc tgcgtcgtgg
     6301 gtgtgtatgc accggtgagc tcgaccacgt tcaggtccag gagctcggtc aaccgccgtt
     6361 tgaacgcgag cgtctcctcg aagtggaaac cggtctcgag gaacagcacg ggcacatccg
     6421 gccggatctg cgtcgccatg tgcatgacgc aggtaccctc ggcctggaag gccgaggcga
     6481 gtgcgatctc gcgcaacccc gactcctcga gcgcccaccg gacgatctcg cgcgggtgcg
     6541 tcggttcgag ccgggcgttc agcgcacgga gcgcttcgtc ggagagcagg gtctcgggca
     6601 tgaggggcct cggcgtgggt gcacggagta tagggccggg gtcggggtcc ttcggctcgc
     6661 ggctatcctt gtgggccgtt caccgcgacg aaaggctgat gtgccatgcc cgacgacacg
     6721 acgatcgccc cgcacggcgg cgacctcgtc gacctgctcg tccccgaggg tcggcgggag
     6781 gccacccgag ccgaggccga ccacctcccc aagctggtgg tcaacgcccg cgagctctcc
     6841 gacctggaga tgctcgccgt cggggccctc tcgccgctga gggggttcgt cggcgaggcc
     6901 gactacctct ccgtcctgga gacgatgcac ctgtcgagcg gcccggcctg ggcgatcccg
     6961 gtcacgttgt ccctgaccga cgacgacgtg aagcggatcg gcgcggggag ctcggtcgcc
     7021 ctgctgccgt gggagggggg cgccccgatc gccatcgtcg acgtcgccga ggtcttcaag
     7081 cgcgaccgcc agaaggaggc gctcgcggtc tacgcgaccg aggacctcga gcacccgggc
     7141 gtgcaggcgc tccacgacgc cggcgacttc tgcctcgccg gcacggttcg cgcgctcgcg
     7201 ctgcccgagc acgacgactt cctgccgtac cggctcacgc ccgcgcagac gcgggccgcc
     7261 ttcgccgagc gaggctggag gaccgtggtg ggcttccaga cccggaaccc gatccaccgt
     7321 gcgcacgagt atctgcagaa gtgcgcgctc gagatcgtcg atgggctcct cgtgcacccc
     7381 ttggtggggg agacgaaggg cgacgacgtt ccggccgacg tgcggatgcg atgctacgag
     7441 gccctcttcg agggctacta cccgaaggat cgcgcgatgg tgtcggtgtt ccccgccgcg
     7501 atgcggtacg cgggcccccg cgaggcgatc tggcacgcga tctgccgcaa gaactacggc
     7561 tgcacgcatt tcatcgtcgg ccgagaccac gcgggcgtcg gctcgtacta cgggacctac
     7621 gatgcccagc ggatcttcga ggagttcgag cccggtgagc tgggcatcac gccgttgatg
     7681 ttcgagcact cgttctggtg caacgcgtgc gaggggatgg cgtcgcccaa gacgtgcccg
     7741 cacggtgagg ggtcgcgggt gtcgctgtcg ggcaccaagg tgcgcgagat gctccgcgcc
     7801 ggcgaacgcc ccccggccga gttcagccgt cccgaggtgg ccgacatcct gatcgaggcg
     7861 atgcgcgagg gctaggggct cccgtttgcc cgctcaccag cgcccttgat agcgtcgagc
     7921 gcgcgccggg cggcggccca gatcgtcagg ggggtgccac gatcgctcgt aggacctcgc
     7981 tccgaacctt cctcggggtg accctcgttg cggcgacgct cggggtcatc ccggccttcg
     8041 ccggcacgca ggatcggctc gacgcggcca aggccgagct cgcgaaggtc caggccgagc
     8101 ttgatcgggc gacggctcac tggcactcgg ccgtcatgcg gctggaagcc actcaagccg
     8161 ggattctcga tgcacgggtc cggatccgca gactcgagga tcgggtcgcc gcggtcgggg
     8221 ctcgactcaa ccgcagggcg gtgatcgcgt tcacgaacgg tccggcgagc accatcgacc
     8281 tgctcctgtc ctcgggttcg ttcaccgact tctccgatcg tctcgaattc cttggatcga
     8341 tggctcaggg agacgccgac cttgtcatcg agcgggaggt cgctcaggag gagcttcgcc
     8401 gggagcgcga caacctcctg gtgctggcag atcgacaggc cgcggttgcg gaggaactcg
     8461 ggcaggcgca ggacgtcatc gatgcccagt tcgaccggat ctccgaccgg gtggccgagc
     8521 tgaccgagca ataccggcag gagatcgccg cctctcggga gctcgtgatc ctggggcaga
     8581 cgccggatcc cgacggctcg atccaggtgt gcccggtcgc ggggcccaac tcgttcatcg
     8641 acacgttcgg gtggcctcgg cccggaggtc gcacgcacca agggatcgac ctgattgcgc
     8701 ccttcggaac cccgatcgtc gccgcccacc cagggagcgt gtcctattca tcgagctcat
     8761 tgggcggact gcaggcctac gtgtatggct ccggcggcta ttcgttctac gcccaccttt
     8821 ccggctacag ctccgcgagc ggaagcgtgg gcgccggcac cgtgatcggg tacgtcggga
     8881 gcacggggga cgcgggatcg acgaaccacc tgcatttcga gtggcatccg ggcggcggcg
     8941 cggccgttga cccgtacctg atgctgctcg cggtctgttg agcacggccg acctcgcatc
     9001 cgccaccggt agcgaagcgt ccggcgcgct acgatgcgtc ctcatgagcg agcaggtcgg
     9061 gcaccgacgg atcgaccgga tccgcacccc cgagttcgtc gagggccttt ccgggctctc
     9121 gctggaggac cttcgggccc gccgcgacga gtgctacgcg gagcgcgaat atctgtcgct
     9181 ccttcgtcgg ctcgtccagg ggcgcgccga gatcctcagg gcggaggtcg agcgtcgcgg
     9241 gagcggcggc aaggcgggca gcctcgtcga tcagctctcg acgatcctgt cctcggaggg
     9301 tcagggaccg tcccggggcg aggcggtcaa ggtcggtctg cccgaggagg agatgctcct
     9361 ggctcgccgg cgcgtcgagc ggttggtggc cgacgccgag atctccgacc cgggcacgct
     9421 cgacgacgag cgtctggccg ccgccgtgga gctgctggtc ggcgaggaac atgaggtgtc
     9481 cgaggcccgc aacgatgtgc tccaggtgat ggacgccctg caggaggagc tcaagcgccg
     9541 gtaccgcgac gacccctcgc tcgtgctttc ctgacccacc cacccgtcct gacccaccca
     9601 cgcgtcctga ccacccatcc gtcctgaccc aaggcctgtc ggccgggtgg gggcgccggt
     9661 gggtcccctg gcgtccgaca ggccatcccg ccggggggag cgtggtggac caggcgaatt
     9721 gggccgccag gcctatggac ccacttcggc cgaaccggca ggctcttccc cgactcgcgc
     9781 accatcctgc gctaccgtgc ggccccgacc gatgagcgaa cgaaaccttc ccgggcacgg
     9841 ccccggggtc cagacggacc ccgaactgcc gagcgtccag gagcagctca tcgtcttcgc
     9901 ccgggagctg ggggagctct atcgcctcga acgatcccgc aacgccgagc tcgagacggt
     9961 cctgg
//
LOCUS       DATBRK010000197         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1764474, whole genome shotgun sequence.
ACCESSION   DATBRK010000197 DATBRK010000000
VERSION     DATBRK010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764474"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /gene="carB"
                     /locus_tag="VIH70_05965"
     CDS             complement(<1..476)
                     /gene="carB"
                     /locus_tag="VIH70_05965"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513329.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEY8115943.1"
                     /translation="MPKRSDLHRILVIGSGPIVIGQACEFDYSGTQACKALRREGFHV
                     SLVNSNPATIMTDPEFADRTYVEPLTRESVLQVIERERPDVLLPTLGGQTALNVAVAL
                     AESGDLERLGVGLIGAGLDAIHRAEDRSGFKETMSRAGLATPSAGTARSLDRALELG"
     gene            complement(476..1618)
                     /gene="carA"
                     /locus_tag="VIH70_05970"
     CDS             complement(476..1618)
                     /gene="carA"
                     /locus_tag="VIH70_05970"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY8115944.1"
                     /translation="MSGGPAAVLVLEDGGVFRGTAFGASGEGFGEAVFNTGMSGYQEV
                     ITDPSYAGQVVAMTAPQQGNYGTNAEDAESGRVHVSGFVVREVARCASNWRATGSLAD
                     ELARADVVGLEGVDTRRLTLHLRDRGAMRCGVSTVDLDADSLLERVRSHPAMEGSDLA
                     MGVSSDEPYAAWNLVGPASAELGRVYRVAAYDFGLKRNILRRLAAAGIEATVVPATTP
                     AAEVRSAGFDGVVLSNGPGDPAATTYGIDACREMLGTMPLFGICLGHQLLGLALGGRT
                     SKLPFGHRGANQPVKDLRTGRVEITSHNHGFAVEPDGWPRDRNLVRTAFGRVELTHWN
                     LNDGTLEGLRCLDVPALSVQYHPEAAPGPHDARYLFDEFRALMAAA"
     gene            complement(1615..2904)
                     /locus_tag="VIH70_05975"
     CDS             complement(1615..2904)
                     /locus_tag="VIH70_05975"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019884819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY8115945.1"
                     /translation="MPEPVLFRGARVIDPASGTDQVADLLVDGGVIAPAASPVATVLD
                     CDGLVLAPGLVDLHTHLRQPGREDKETVESGCRAAAHGGFTAVSPMANTDPVADNAAV
                     IHEVLELARAAGTCDVFPVGAITRGLGGEEMAELGEMVDAGVRLFSDDGRCVPNARML
                     RNALTYVRAFEGVVIAEHCEDGSLAEGGQMHEGLTSSSLGLAGQPREAEEVVVARDLA
                     VARLTEGRLHLCHLSSAGSVEMLRRAKAEGLRVTAEVTPHHLVFSEEDLLTYDTNMKM
                     NPPLRSGQDRRELRAALTDGTIDAVATDHAPHAVEEKEAEFDQAPFGTIGLETALAAV
                     LTHLVEPGELTLARAIEAMSTAPARILGAGDQGGPIEPGRPANLFVFDPSAEWAVEPP
                     FASKSRNSAFLGRRLRGRVVHTMLRGELVVADGKAQR"
BASE COUNT          422 a         1117 c         1064 g          430 t
ORIGIN      
        1 ccgagctcga gcgcgcggtc gagcgagcgc gcggtccccg cgctcggcgt cgcgagcccg
       61 gcgcggctca tcgtctcctt gaagccgctc cgatcctcgg cgcggtggat cgcgtccagc
      121 ccggcgccga tcagtccgac cccgagccgc tcgaggtccc cggattccgc gagcgcgacc
      181 gccacgttca gcgcggtctg gcctccgagg gtcggcagga gcacgtcggg acgctcacgt
      241 tcgatcacct gcaggaccga ctcgcgcgtg aggggttcga cgtacgtgcg gtccgcgaac
      301 tcgggatcgg tcatgatcgt ggccgggttc gagttcacga gcgacacgtg gaacccctcc
      361 cgccgcagcg ccttgcaggc ctgggtcccc gagtagtcga actcgcacgc ctgcccgatc
      421 acgatcgggc ccgagccgat caccaggatg cggtgcaggt cggatcgttt cggcatcagg
      481 ccgccgccat cagcgcgcgg aactcgtcga acaggtagcg cgcgtcgtgg ggtccggggg
      541 cggcctcggg gtggtattgc acggagagcg cggggacgtc gaggcaccgc agcccctcga
      601 gcgtcccgtc gttcaggttc cagtgggtga gctcgacgcg cccgaacgcc gtccgcacga
      661 ggttccgatc gcgcggccac ccgtccggct cgaccgcgaa cccgtggttg tggctcgtga
      721 tctcgacgcg gccggtccgc agatccttca cgggttggtt cgcaccgcgg tgcccgaacg
      781 gcagcttgga cgtccgaccc ccgagcgcga gcccgaggag ctggtgcccc aggcagatgc
      841 cgaacaacgg catcgttccg agcatctcgc ggcacgcatc gatcccatag gtcgtcgcgg
      901 cggggtcacc cggcccgttc gacaggacca cgccgtcgaa cccggcggag cggacctcgg
      961 cggccggcgt cgtcgccggg acgactgtcg cctcgatccc ggccgccgcg agccggcgga
     1021 ggatgttccg cttaagaccg aagtcgtacg ccgccacgcg atacacccga ccgagctcgg
     1081 cgctcgccgg tccgacgagg ttccacgcgg cgtagggctc gtccgagctc acgcccatcg
     1141 cgaggtcgga accctccatc gccggatgcg aacggacgcg ttcgagcagc gagtccgcgt
     1201 ccaggtcgac cgtcgagacg ccgcatcgca tcgcgccccg gtcgcggagg tgcagtgtga
     1261 ggcggcgggt gtcgacgccc tcgagcccca cgacgtcggc gcgcgccagc tcgtcggcga
     1321 gcgacccggt ggctcgccag ttggaggcgc agcgcgcgac ctcgcgcacc acgaaccccg
     1381 acacgtgcac gcgaccggat tcggcgtcct ccgcgttcgt gccgtagttg ccctgttgcg
     1441 gtgcggtcat ggccacgacc tgccccgcgt aggagggatc ggtgatcacc tcctggtacc
     1501 ccgacatccc cgtgttgaag accgcctcgc cgaacccctc cccggacgca ccgaacgcgg
     1561 ttccgcggaa gacgccgccg tcctccagaa ccaggaccgc cgcgggtccc ccgctcaccg
     1621 ctgagccttc ccgtcggcca cgacgagctc cccgcggagc atcgtgtgga cgacccgtcc
     1681 ccgcagcctg cgaccgagga aggcggagtt ccggctcttc gaggcgaacg gtggctccac
     1741 cgcccactcg gcggacgggt cgaagacgaa gaggttggcc gggcggccgg gttcgatcgg
     1801 cccgccctgg tccccggcgc cgaggatccg cgcgggcgcg gtggacatgg cctcgatcgc
     1861 gcgggcaagc gtgagctcgc cgggttcgac cagatgggtg aggacggccg ccaacgcggt
     1921 ctcgagcccg atcgtgccga acggcgcctg gtcgaactcc gcttccttct cctcgacagc
     1981 gtggggagcg tggtcggtcg cgacggcgtc gatcgtcccg tccgtgagcg cggcccggag
     2041 ctcgcggcga tcctggccgg aacgcagcgg cgggttcatc ttcatgttcg tgtcgtaggt
     2101 gaggaggtcc tcctcgctga acacgaggtg gtgcggcgtg acctcggccg tgacgcgcaa
     2161 accctcggcc ttggctcggc gcagcatctc gaccgagccg gcggacgaga gatggcacag
     2221 gtgcaaccgg ccctccgtca gtcgcgcgac cgccaggtcg cgtgcgacca cgacctcctc
     2281 tgcctcccgc ggctggcccg cgagcccgag cgacgacgag gtcagaccct cgtgcatctg
     2341 tcccccctcg gccaacgagc cgtcctcgca gtgctccgcg atcaccacgc cctcgaacgc
     2401 gcggacgtac gtgagcgcgt tgcggagcat gcgcgcgttc ggcacgcaac gtccgtcgtc
     2461 gctgaacagg cggacccccg cgtcgaccat ctcgccgagc tcggccatct cctctccgcc
     2521 gaggccgcgc gtgatcgcgc cgacggggaa gacgtcgcag gttccggcgg cccgggcgag
     2581 ctcgaggacc tcgtggatca cggccgcgtt gtcggccacc ggatcggtgt tggccatcgg
     2641 ggagaccgcg gtgaatccgc cgtgcgcggc cgcgcggcat cccgactcga cggtctcctt
     2701 gtcctcgcgg ccgggttgcc gcaggtgggt gtggaggtcg acgagccccg gtgccagcac
     2761 caggccgtcg cagtcgagca ccgtcgcgac cgggctcgct gcgggcgcga tgacgcctcc
     2821 gtccacgagc aggtcggcga cctggtccgt cccggaggcc ggatcgatca cgcgggcgcc
     2881 cctgaacagg accggttcag gcacccgtgg ccacctcctc cggcagccgg acggcgcggg
     2941 cctcgtcggc cgcctgggag ccggaccctc cgaggaggag gtacaggagc gacatccgaa
     3001 cggcgatccc gttcgtcacc tgctcctcga tga
//
LOCUS       DATBRK010000198         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1476888, whole genome shotgun sequence.
ACCESSION   DATBRK010000198 DATBRK010000000
VERSION     DATBRK010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476888"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VIH70_05980"
     CDS             <1..339
                     /locus_tag="VIH70_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY8115946.1"
                     /translation="ASQTPAIRSGQRIIFLTLDDATGPVDVTVFESVQPKVAKTVFHS
                     FVLAVVGTVRRTGYKGVSVVAEDVWDLTALLHARRQGRLAQALAHEGPSPGAAVPRKL
                     WHSSGGSAGR"
     gene            381..1562
                     /gene="dinB"
                     /locus_tag="VIH70_05985"
     CDS             381..1562
                     /gene="dinB"
                     /locus_tag="VIH70_05985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY8115947.1"
                     /translation="MTRTRSSEPILHVDLDAFYASVETLKDPSLSGRPVAVGGAGTRG
                     VVMSASYEARAFGIRSAMPAVRARRLCPDVIFVPPDFESYKVHSNRFREILLAVTPAV
                     EPISLDEAFLDVSGAHLLFGEPEEIGRKVRREVQDGIGVRCSVGVAPTKLVAKLASRD
                     AKPDGMVVVRADEVDAYLEPLPVRALWGVGEKTAETLGRLGVRTVGDLGRTPVGVLGR
                     LMGERHARDLRDLARGVDDRTVVPYEAPKSVSHEETFDRDLDDRRELLREVLSLSQRV
                     AARLREDGFRTRTVTLKVRLANFTTLTRSRTLAAPTDVAADLYEVVGELFTALPGARR
                     RVRLLGVAATGLVPAGEEQLALLRSERWGDVERAVDRIDRRFGPGSAVPAALLERGRD
                     R"
BASE COUNT          201 a          538 c          601 g          232 t
ORIGIN      
        1 gcgtcgcaga cgccggcgat ccgcagcggc cagcggatca tcttcctcac gctggacgac
       61 gcgacggggc cggtcgacgt gaccgtgttc gagagcgtgc agccgaaggt cgccaagacc
      121 gtgttccact cgttcgtgct cgccgtggtc gggaccgtcc gacggaccgg gtacaagggt
      181 gtctcggtgg ttgccgagga cgtgtgggat ctgacggcgc tgctgcacgc ccggcggcag
      241 ggacggctcg cgcaggcgct cgcccacgag gggccatccc ccggcgcggc cgtcccccgc
      301 aagctctggc attcgagcgg cggctcggcc ggccgatgac gtcgctcgtc acgtccggcg
      361 cggcggtacg ctccgcgacg atgacccgca cgaggtcgag cgagccgatc ctgcacgtcg
      421 atctcgatgc gttctacgcc tcggtcgaga ccctgaagga cccgagcctt tcgggcaggc
      481 cggtcgcggt gggcggcgcc gggacgcgag gggtcgtgat gtcggcgtcc tacgaggccc
      541 gggcgttcgg catccggagc gcgatgcccg ccgtgcgcgc tcggcgcctg tgtccggacg
      601 tgatcttcgt cccgcccgac ttcgagtcct acaaggtgca ctccaaccgc ttccgcgaga
      661 tcctgctggc ggtcacgccg gcggtcgagc cgatctcgct cgacgaggcg ttcctcgacg
      721 tctccggtgc gcacctgctg ttcggcgagc ccgaggagat cggacgcaag gtccgcaggg
      781 aggtccagga cggcatcggg gtgcggtgct cggtgggcgt ggcgcccacc aagctggtcg
      841 ccaagctcgc ctcgcgcgac gcgaagcccg atgggatggt cgtcgtgcgc gccgacgagg
      901 tggacgccta cctcgaaccg ctgcccgtgc gggcgctctg gggggtgggc gagaagacgg
      961 ccgagaccct cgggcggctc ggtgtgcgga ccgtcggcga tctgggccgg accccggtcg
     1021 gggtgctcgg caggctcatg ggcgaacggc acgcgcgcga cctcagggac cttgcccgtg
     1081 gggtagacga ccggacggtc gtgccctacg aggcgccgaa gtcggtcagc cacgaggaga
     1141 ccttcgaccg cgacctggac gatcggcgcg agctgctccg cgaggtgctc tcgctctccc
     1201 agcgggtggc cgcccggctt cgcgaggacg ggttccggac ccggacggtc accctgaagg
     1261 tccggctcgc gaacttcacg acgctcacgc gttcgcgaac gctcgcggcg ccgaccgacg
     1321 tcgccgcgga cctgtacgag gtggtcgggg agctgttcac cgctttaccg ggggcccgcc
     1381 ggcgcgtccg attgctcggt gtggccgcaa cgggcctggt gccggcgggg gaggagcagc
     1441 tcgcgctgct gcggagcgag cggtggggcg acgtcgaacg cgcggtggat cggatcgatc
     1501 ggcggttcgg accgggatcg gccgtgcctg cggccctgct cgaacgcggc cgcgatcgct
     1561 gaacgtcccg cc
//
LOCUS       DATBRK010000199        12812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_315827, whole genome shotgun sequence.
ACCESSION   DATBRK010000199 DATBRK010000000
VERSION     DATBRK010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315827"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VIH70_05990"
     CDS             complement(<1..1295)
                     /locus_tag="VIH70_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8115948.1"
                     /translation="MDRPMTRYATTDDGVSIAYQVVGEGPVDLVFVHSFVSHVEVFWE
                     LPAFERFVEGLSSWARVILFDKRGVGLSDRLSVIPTIEARLDDLRAVLDAVGSERTLV
                     VGNNDGGALAALFAATYPERTLGLVLWRGGVRTARAPDYPWGLSEDQFEERLRRRMVI
                     WGDESMGEEAARMSYPGIGERLAGDPAFVRWRTRLQRYAASPGDLARFSRVWFTTDAR
                     SVLPLIQVPAAVMMRVGWIDAYVQEARWTADQIPGAQVVQVPGDGDDVYLGNVGEVAE
                     AIRGFAQSIEEEHAAFDRVLATVVFTDIVGSSKRAADLGDQAWKQLVEQHHARVRALL
                     ARYRGHEVDTAGDGFFATFDGPGRAVRCAQAIVRSVSTLGIEVRAGVHTGEVETIDGK
                     AGGLAVVIGARIGGLAGASEVLSSQTVRDLTAGSGIVFE"
     gene            complement(1471..1567)
                     /locus_tag="VIH70_05995"
     tRNA            complement(1471..1567)
                     /locus_tag="VIH70_05995"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1508..1510),aa:Val,seq:gac)
     gene            complement(2228..3430)
                     /gene="ugpC"
                     /locus_tag="VIH70_06000"
     CDS             complement(2228..3430)
                     /gene="ugpC"
                     /locus_tag="VIH70_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017586874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8115949.1"
                     /translation="MAKIVLDSVTKVFGSDVIAVNDVSVEIGDGEFMVLVGPSGCGKS
                     TILRLLAGLEELTAGEIFIDDRQVTDLPPKDRDVAMVFQNYALYPHMTVEQNLGFGLK
                     LRKTAKDETSRRVRDAARILGLEPLMQRKPAELSGGQRQRVAMGRAMVREPVAFLMDE
                     PLSNLDAKLRVQMRAQLAQLHDRLRTTTIYVTHDQIEAMTLGKRVAVLRDGVLQQVDT
                     PQTLYNRPANLFVAAFIGSPPMNLVEADVHDGRIAFAGFELPLGTDAGLRTYEGSSVI
                     LGIRPPDLEDTDVWSNSALPSIEVVADVTEDLGSEVNVLFRIDAPPVATEELSAAVDD
                     PSGEAISLLGEESRATFCARVDARTSVRSGRTIRLSVDPARFHFFDPQSGDAVEAAAP
                     AAIGARGV"
     gene            complement(3439..4440)
                     /locus_tag="VIH70_06005"
     CDS             complement(3439..4440)
                     /locus_tag="VIH70_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018487213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY8115950.1"
                     /translation="MTRPAVLAVDGGGSKIEVALVGRNGDVLGASRIANPDPEHGSPY
                     LDRIVHAVESAVAGANLDPERRPVAPVGVFCLAGADLGDDERRLLRWLSKQAWAEEDV
                     IRNDTFAIMRTGSDRSWGVGVVCGTGTNCAAIAPDGRTFRIPALGQISGDWGGGGDLG
                     RDALWYAVRAEDGRGSNTELARLVPAQFDLRRPRQVTEAIHFGRLEEARLSELTPLVF
                     RAASRDPVARSLVDRQADEIVTMAGTAIRRLDLGQLDVHVTLGGGVLRNRFAPFLERI
                     RTGIRTIAPLAQVSVVTDPPVVGAALIGLDRAGAPSSGHRRLRAVLTNERLRTHTRR"
     gene            complement(4437..5063)
                     /locus_tag="VIH70_06010"
     CDS             complement(4437..5063)
                     /locus_tag="VIH70_06010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8115951.1"
                     /translation="MSDLLRPETRVALTFDAEHPDRSNASAENAGRVLDALAAAHVRA
                     TFFVQGRWARSEPETLLRIANEGHVVGNHSNYHVRMPLLSDEGLRRDITEGEDAIREV
                     GCVDPRPWFRCPFGAGRQDPRVLGAIEGLGYRDVAWDVELEDWEPWRSAEQIADDAVK
                     GSLARGDGAVVLLHTWPGHSAQAVPAIIDRLGKEGVRFVSIDELDEFP"
     gene            complement(5056..5490)
                     /locus_tag="VIH70_06015"
     CDS             complement(5056..5490)
                     /locus_tag="VIH70_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY8115952.1"
                     /translation="MRVGGVLVVLGRGPARAAVEMGALMLKESGVMAESLESAAFRHG
                     PLELVGPGMSAIVMATEPETRRLDLGLAADLVSSGAQVVVITPDGEDPDGTYGVATGF
                     VDRALMSAISIIPVQLLAWRLAVAHGRSLGIYTRASKVTTRE"
     gene            5774..6082
                     /locus_tag="VIH70_06020"
     CDS             5774..6082
                     /locus_tag="VIH70_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115953.1"
                     /translation="MRLDHVGQLDDLGALAGLAHDHHERSCAEDRAAEMEQLGRVHED
                     GGDTAPGEFVHCRIARIVGAPHPGEDDRRRQTAGLVDALELVPLLDQRGGGAPEPVRL
                     "
     gene            6162..7463
                     /locus_tag="VIH70_06025"
     CDS             6162..7463
                     /locus_tag="VIH70_06025"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEY8115954.1"
                     /translation="MKLVVVGAGSTYTPELVEGIARRADRLPVDDLVMLDIDAARLEI
                     VGGLARRMLTKAGWPGLVSLTGDRDRALEGADFVIVQLRVGGNQARLLDETIPLKHGC
                     IGQETTGPGGFMKALRTVPVVLELAEETARRAANGAWIVDFTNPTGIVTQALLDEGHR
                     AVGLCNFAINVQLAAAALFGIDLGRVKLDHVGLNHLSWVRRILVDGDDRLPDILEAIS
                     GEGDPDLRARVSEAGVVSVHPPDLIRLLGAIPSYYLRYYYATDRALQEQLEGHIRAQE
                     VIEIEAELLERYRDPALDTKPELLERRGGAHYSEAAAMLVASLHDGAADVQVANVRND
                     GAIPNLPGDDVVEMSARISRDGAHPVPTEPLAPELLGLVQQVKSYERLAIDASTTGSR
                     RRALEALMANPLVRQYELAEALLRDLLEANRAHLPRFFPRG"
     gene            7599..8750
                     /locus_tag="VIH70_06030"
     CDS             7599..8750
                     /locus_tag="VIH70_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY8115955.1"
                     /translation="MLRAINERTVLERIRAVGPASRAQIARGTGLSKPTVSQALQTLE
                     RAHLVREAGRSSGAKGPTALLYELNPRAGWVVGIDVGRDLVHAVLADLNGDIVARRDE
                     RAHARSAGALIAQIGELAHGLAADADLRWRQVTQAAIGSPGVFDPLRDQVVLAHNLPG
                     WGRPGLVEALQKELGTNIAFENDVNLAAVGEQTHGLGKDVPNFAYLHVGTGVGLGLVL
                     NGELYRGSRGAAGEVAYLPLTTTDPHEPGSRRRGALETALGTAGIVATAQRLGMTSSV
                     TPQRVFAAARRGDRTAGRVTSAVAERIALTIAAIVPIVDPELVVLGGAIAGNGDLLLD
                     AVDRELHEISPFRPRVEVSMLGEDAVIRGAVAVALQAAQEQLFSRTART"
     gene            8797..10137
                     /locus_tag="VIH70_06035"
     CDS             8797..10137
                     /locus_tag="VIH70_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8115956.1"
                     /translation="MRRIFLAACAAVLFAAACTAGGGTSSSPTVVHTIDPNVSHQPVT
                     LVVWDWFRQRELENLQQITAAFEQQYPWITLQFVPGKHTGEYVRAINADVPIDVAIDW
                     GPDNIGKYCSTGAWVDLAPYIKAYGLDMDALFPGSALTYTRYGGVQCSLPVLTDAYGL
                     YYNKDMFAEAGIPGPPKTLSELTEDAKKLTELNPDGSIKVAGFVPISTFYENPNLYDG
                     NPWGAQWYQDDGSSAFASDPAWAEMAQWDKELTDWYGYDKLQTFYEDIGGPDSEWSSA
                     HAFEQGKIAMHYDGEWRVSFIDNDNASVNYGTAPFPVADDKPDLYGSGQVGGTIIGIP
                     RTAEHPDEAWLLVQWLSTQTKPLNQLADLLKNVPTTKESLQTTALGEDPQFEPFLGIY
                     QDPASYHYPLKPSGSADADALAAFLEDWEAGDVADLQQGLQDLAEHVDAQQQLG"
     gene            10221..11099
                     /locus_tag="VIH70_06040"
     CDS             10221..11099
                     /locus_tag="VIH70_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8115957.1"
                     /translation="MAPAVFMSPWVVGFVIFILYPMLATLYFSFTKYNLLSPPQWVGL
                     FNYRFMFTSDPNFWQAVRNTLWIIAVGTTSGIVFAIAAAMVLTRVRAGSGVYRTVFFV
                     PTMVPAVAGALAFVFLLHPSGPVNSILGLLHLPQPLWFRDPTWSKPALVLLGLWGIGD
                     TMIIFLAAMLDVPIHLYEAADIEGAGAWQKFRHITLPMISPVIFFAVVIGIITGFQYF
                     AQAYVASGGTDLTLGQPQGSLMFYSIWLYNQGFEYGNMGYAAAMAWVLFVIVMACTLI
                     LIRTSRRWVHYQGGFR"
     gene            11069..11995
                     /locus_tag="VIH70_06045"
     CDS             11069..11995
                     /locus_tag="VIH70_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8115958.1"
                     /translation="MGPLPGGVPIATATASPLSIADIRPRAIPPAARRRRFLVAVANH
                     SIAIALGTVFVLPFVFVLLTALMARSQALSGTLWPRPFVLDNFVRVFRFIPLWQYTWN
                     TVLYAGLSTLGTVASSIPVAYALSRMRWRGRQVAFVLVLSTLMLPPQVTIVPLYVIFV
                     RIGWIGSLKPLIVPAFFGDAFTIFLLRQFFMTIPEELSDAARVDGASEFQILRRVIIP
                     LAKPAIAAVALFQFLYCWNDFFGPLLYTGSNPKIWTLAVALSEFSSVLHQGVLWNIQM
                     AASVLFMLPVIALFFVAQRAFIEGVTLTGVKG"
     gene            complement(12642..12716)
                     /locus_tag="VIH70_06050"
     tRNA            complement(12642..12716)
                     /locus_tag="VIH70_06050"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12682..12684),aa:Val,seq:gac)
     gene            complement(12744..12812)
                     /locus_tag="VIH70_06055"
     tRNA            complement(12744..12812)
                     /locus_tag="VIH70_06055"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12780..12782),aa:Cys,seq:gca)
BASE COUNT         2028 a         4372 c         4259 g         2153 t
ORIGIN      
        1 tcctcgaaca cgatccccga gccggccgtg agatctcgca ccgtctgcga gctgagcacc
       61 tccgacgccc ccgcgagtcc gccgatcctc gccccgatca cgaccgcaag cccgccggct
      121 ttgccatcga tcgtctcgac ctcgccggtg tgcacaccgg cgcggacctc gatcccgagc
      181 gtgctcaccg accggacgat cgcctgcgcg cagcggaccg cgcggccggg cccgtcgaac
      241 gtcgcgaaga agccatcgcc ggccgtgtcg acctcgtggc cgcggtagcg tgcgagcagc
      301 gcccggaccc tcgcgtgatg ctgctcgacg agctgcttcc acgcctggtc gccgagatcc
      361 gccgctcgct tcgacgagcc gacgatgtcg gtgaacacca cggtggcaag cacccgatcg
      421 aacgccgcgt gctcctcctc gatcgattgg gcgaatcccc ggatcgcctc cgcgacctcc
      481 cccacgttgc cgaggtacac gtcgtcccca tcgcccggca cctggacgac ctgcgcgccg
      541 gggatctggt ccgcggtcca cctcgcctcc tgcacgtagg cgtcgatcca tccgactcgc
      601 atcatcacgg cggcgggcac ctggatcagc gggagcacgc ttcgcgcatc ggtcgtgaac
      661 cacacacgac tgaaccgcgc caggtcgccc ggtgaggccg cgtagcgctg gagccgggtc
      721 cgccatcgca cgaaggccgg atccccggcc aggcgctcgc cgatgccggg gtacgacatc
      781 cgggccgcct cctcgcccat cgactcgtcg ccccagatca ccatccgccg acgcagccgc
      841 tcctcgaatt ggtcctcgga cagaccccac gggtagtccg gagcgcgggc cgttcggaca
      901 ccgccgcgcc agagcaccag gccgagggtc cgctccggat acgtggcggc gaacagggcc
      961 gcaagggctc ccccgtcgtt gttcccgacc acaagggtcc gctcggatcc gaccgcgtcg
     1021 aggactgccc ggaggtcgtc gagccgcgcc tcgatcgtcg ggatcaccga cagccggtcc
     1081 gacaggccga cgccgcgctt gtcgaacagg atcacccgag cccatgagct caagccttcg
     1141 acgaagcgct cgaacgccgg caactcccag aacacctcga cgtgcgagac gaacgagtgc
     1201 acgaacacga gatcgaccgg gccctcgccg acgacctggt aggcgatcga gacgccgtcg
     1261 tcggtggtcg cgtacctcgt catgggccgg tccatgtgcg catcctcgcc gcggccgagg
     1321 cgctcgacaa gccgtgtcgg acccctggga gctgccccag aacgtcgttc cgcgcgtcgc
     1381 gtcaaggttt cgtcacgtcc agggtttcga gccgttgcgt gcttggctcg agcacatcag
     1441 aaaaggccct gtgaccaggg cctttgtcgg tgggcgctag aggatttgaa cctctgacct
     1501 cttccgcgtc aaggaggcga aggaggtttc gaaggctgct ggtcagagca ccgcactcac
     1561 caaaccctct ggtcagagcc ccttctgcca agctctgtcg attcccgtgg gtcacgcctc
     1621 gccccgccgt tccgtcaact tttcgtcaac acgtaggtgc ggccggcgtt ccttaccggc
     1681 gtcgccgggc cactcctcag cccgcaaccc gcacaaccag ctgtctcaac tgtggacact
     1741 tcctttcgcg ataacagatc aaccgacctt ggcgcatgga cggggtagag acgaggactc
     1801 gctcccttcc tcggcccgga cgtcatggtt ccttggagtt gcggtcgatc agcaaaggga
     1861 agagttgctt gccgcgccca gccagaaggt ggaggtttcc cgtgccgtcg ccgagacgtc
     1921 gtgggatgcg cgaccccaag gaatcaggtc gggcggtcgg acagcaacgc gatcatcggg
     1981 cataccgagc agggggcgtc aggaagccgc acagtgataa gtggcacgtg ctggtgcagt
     2041 tcgcgatccc gcgccgtgat gagccactcg ggtggaagcg cgcacccctg acatgcggcg
     2101 cgctcccgcc ggttcggtcc tcgcgattgt cggcgttttc gggactggga cgggctcgcc
     2161 acccaagggg acaggactcc gtgcttgttc cttcgcagat cagcgaaagt caacgagtcg
     2221 acccgcttta gactcctctg gcgccgatcg cggccggtgc ggcggcttcc acggcgtcgc
     2281 cggactgcgg atcgaagaaa tggaaccggg cgggatctac ggacaggcgg atcgtcctgc
     2341 cgcttcgcac gctggtccgg gcgtccaccc gggcgcagaa tgttgctcgg gactcctcac
     2401 cgagcaggct gatcgcctcc cccgatggat cgtcgacggc ggctgagagc tcctcggtcg
     2461 cgaccggcgg ggcgtcgatc cggaagagca cattcacctc ggagccgaga tcctcggtca
     2521 cgtcggcgac cacctcgatc gaggggagcg ccgagttgga ccagacgtcc gtgtcctcga
     2581 ggtccggcgg tcggatcccg aggatcaccg agctcccctc gtaggttcgg agcccggcgt
     2641 cggtcccgag cgggagctcg aaaccggcga aggcgatccg tccatcgtgc acgtccgcct
     2701 cgacgaggtt catcggcggc gagccgatga acgccgcgac gaagaggttc gccggacggt
     2761 tgtacagcgt ctgtggcgtg tcgacctgct gcagaacccc atcgcggagc accgcgaccc
     2821 gcttgcccag tgtcattgcc tcgatctgat cgtgggtgac gtagatcgtc gtcgtacgca
     2881 gacggtcgtg cagctgcgct agctgtgcgc gcatctggac gcgcagcttg gcgtccaggt
     2941 tcgatagcgg ctcgtccatc aggaacgcga ccggctctcg aaccatggcg cggcccatcg
     3001 cgacccgctg tcgctgaccg ccggagagct ccgccggctt gcgctgcatc agcggctcga
     3061 gcccgaggat ccgcgcggcg tcccgcaccc gccggctggt ctcgtccttg gcggtcttcc
     3121 ggagcttcaa tccgaatccg aggttctgct cgaccgtcat gtgtggatac agggcgtaat
     3181 tctggaacac catcgcgacg tcgcgatcct tgggggggag gtccgtgacc tgacggtcgt
     3241 cgatgaagat ctcgccggcc gtgagctctt cgagcccggc gaggagccgg aggatcgtcg
     3301 actttccgca accggagggg ccgaccagta ccatgaattc gccgtcgccg atctcgaccg
     3361 agacgtcgtt cacggcgatc acgtccgagc cgaagacctt cgtgacgctg tccagcacga
     3421 tcttggccat ctcccctcct atcgccgagt gtgggttcgt agacgctcat tcgtcaagac
     3481 ggctcgcagc cgccggtggc cggagctcgg cgcgccggcg cgatcgaggc cgatcaacgc
     3541 ggccccgacc accggcgggt ccgtcaccac cgagacctga gccagcggcg cgatcgttcg
     3601 gatccccgtt cggatccgct ccaggaacgg cgcgaatcgg ttgcgcaaca caccacctcc
     3661 gagggtgacg tgcacgtcga gctggcccag gtcgagccgg cggatcgcgg tcccagccat
     3721 cgtcacgatc tcatccgctt gccgatcgac caggctacga gcgaccggat cccgtgatgc
     3781 cgctcggaat accaacggtg tgagctcgga caagcgcgcc tcttcaagcc gtccgaagtg
     3841 gatcgcctcg gtgacctggc gcggtcgacg caggtcgaac tgagcgggga caagcctcgc
     3901 gagttccgtg ttcgatcctc gcccgtcttc ggcccgaacc gcataccaga gggcgtcgcg
     3961 accgaggtcg ccgccgccgc cccagtcgcc cgagatctgg ccgagggcgg ggatccggaa
     4021 ggtccggccg tccggcgcga tcgcggcgca gttggtcccg gtgccacaga cgaccccgac
     4081 accccacgat cggtccgaac cggtgcgcat gatagcgaac gtgtcgtttc ggatcacgtc
     4141 ctcctctgcc cacgcctgct tcgatagcca gcgcagcaac cgccgctcgt cgtctccgag
     4201 gtccgccccc gcgaggcaga agacaccgac cggcgcgacc ggacgacgct ccgggtccag
     4261 gtttgcacca gcgacggcgc tctcgacggc gtgcacgatg cggtcgaggt agggcgagcc
     4321 gtgctcggga tcgggattgg cgatccgaga ggccccgagc acatcgccgt tccggcccac
     4381 gagtgcgacc tcgatcttcg agccgccgcc gtcaacggcg agcacggccg gcctggtcac
     4441 gggaactcgt ccagctcgtc gatcgagacg aagcggacgc cctctttacc cagccgatcg
     4501 atgatcgcgg gaaccgcctg ggccgaatgg ccgggccacg tgtgtaggag gacgacggct
     4561 ccgtcgcccc gcgcgagcga acccttcacg gcgtcgtcgg cgatctgctc ggcgctcctc
     4621 catggctccc agtcctcgag ctcgacgtcc catgcgacgt cgcggtaacc gagcccttcg
     4681 atggcgccaa gcacgcgcgg atcctgccgg ccggcgccga aagggcagcg gaaccacggc
     4741 cgaggatcga cgcagccgac ctcgcggatc gcgtcctcgc cttcggtgat gtcgcgccgt
     4801 aggccctcgt ccgagaggag gggcatccgc acgtgatagt tcgagtggtt gccgacgaca
     4861 tggccttcgt tcgcgatccg gagaagcgtc tcgggctcgg agcgcgccca gcgaccctgc
     4921 acgaagaacg tcgctcggac gtgggccgct gccagcgcat cgaggacccg gcccgcgttc
     4981 tcggccgagg cgttggagcg atccggatgc tcggcgtcga aggtgagcgc cacgcgcgtc
     5041 tctggccgga ggaggtcact cacgggtcgt gaccttcgac gcgcgggtgt agattcccag
     5101 tgaccgcccg tgcgcgaccg cgagccgcca cgcgagcagt tgcaccggga tgatcgagat
     5161 cgccgacatc agggcccggt cgacgaagcc cgtggccacg ccatacgtgc cgtccgggtc
     5221 ctcgccgtcg ggcgtgatga cgacgacctg cgcccccgag ctcaccaggt cggccgccag
     5281 gccaaggtcg agtcgacggg tctcgggctc cgtcgccatc acgatcgccg acatccccgg
     5341 acccacgagc tccagtgggc cgtggcggaa cgccgcggac tcgagcgatt cggccatcac
     5401 gccgctctcc ttgagcatga gcgcgcccat ctcgaccgcc gcgcgcgccg ggccgcgtcc
     5461 caggacgacc aggaccccgc cgactcgcat gagctcggcc agtcgggtgg cctcggcccc
     5521 gggaggcgtc gagcaggcgt tcggccgaca gggcggcggc ctcagccgcg gtccaggtcc
     5581 ggtcgaccgc ggtccccacg ctgtcaccgc cgaggaggcg cgccacgccc gagagcgtcg
     5641 tgagggctgc cgagaacgtg acggtcgatg gcccgatttc gagccccgcc gtcgtgtcga
     5701 ggcggacgtc cgctcggggc gcgagctcgt tggccaggcc gttggtcacc gacacgacga
     5761 acggtcggca gcgttgcgcc tcgatcacgt cggccagctt gacgatctcg gcgctctcgc
     5821 cggactggct cacgatcacc acgagcgttc gtgcgcagag gatcgcgcgg cggaaatgga
     5881 gcagctcggc cgtgttcacg aggatggcgg ggacaccgcg cccggcgagt tcgttcactg
     5941 ccggatagca cgcatcgtag gagcccccca tccaggtgaa gacgatcgcc gccgccagac
     6001 cgcgggcctc gttgacgcgc tcgagctcgt cccgctgctc gatcaacgag gtggcggcgc
     6061 gccggagccc gtccggctgt gagcagatct ccgcaaggaa tgggtcagga aagtttccta
     6121 acgatttcac ggagtgagtg tacgatgccc gtgccatggg tgtgaagctc gtcgtcgtcg
     6181 gcgccggcag cacgtacacg cctgagctcg tcgagggcat cgcgcgtcgc gccgatcgac
     6241 tgcccgtcga cgatttggtg atgctggaca tcgacgcagc gcggctcgag atcgtcggtg
     6301 gtctcgcccg acggatgctc acgaaggcgg gctggcccgg gctcgtgtct ctgaccgggg
     6361 accgggaccg ggcgctcgag ggagccgact tcgtgatcgt gcagctccgg gtcgggggca
     6421 accaggcgcg tctcctggac gagaccatcc cgctcaagca cggctgcatc ggccaggaga
     6481 ccaccggacc gggcgggttt atgaaggccc tacgaacggt gccggtggtg ctcgagctcg
     6541 cggaggagac cgcccgtcgc gccgcgaacg gcgcttggat cgtcgacttc acgaacccaa
     6601 cggggatcgt gacgcaggcg ctcctggacg aagggcaccg ggcggtcggg ctctgcaact
     6661 tcgcgatcaa cgtgcagctc gctgccgcag cgctgttcgg catcgacctt ggtcgcgtaa
     6721 agctcgatca tgtgggtctg aatcacctct cgtgggtccg gagaatcctc gtcgacggag
     6781 acgaccggct ccccgacatc ctcgaggcga tctccgggga gggcgacccg gacctccggg
     6841 ctcgcgtgtc cgaggccggc gtcgtgagcg tccatccgcc cgacctgatc cgcctgctgg
     6901 gcgcgattcc ctcctattac ctgcgctact actacgcgac cgaccgggcc ctgcaggagc
     6961 agctcgaggg ccacatccgc gctcaggagg tgatcgagat cgaggccgaa ctcctcgagc
     7021 gatatcggga tccagccctt gataccaagc ccgagctgct cgagcgccga ggcggggcgc
     7081 actacagcga ggcggccgcc atgctcgtcg cgtcgctaca cgacggtgcc gccgacgtcc
     7141 aagtcgcgaa cgtgcgcaac gacggcgcga tcccgaacct ccccggcgac gacgttgtcg
     7201 agatgtcggc ccgcatctcg cgggacgggg cgcaccccgt gcctaccgag ccgctggcgc
     7261 ccgagctgct cgggctcgtc cagcaggtaa agtcctacga gcggctcgcg atcgatgcat
     7321 ccaccaccgg gagcagacga cgagccctcg aggcgttgat ggccaatccc ctggtccgtc
     7381 agtacgagct tgctgaggcg ctgctgaggg atctgttgga ggccaaccga gcgcacctgc
     7441 cgcgcttctt ccctcgcggc tgaaaaccgg aggcctcgcc ttgaccttcg aaaggacgtc
     7501 ggcgtaccat ccccggaata gttaggaaac tttcctccac atctctttgg aggaccgaga
     7561 cgagcgcagc caaccgattc tccgcgggga cgccgagtct gctccgcgcg atcaacgaac
     7621 ggacggtcct cgagcgcatc cgggcggtcg gcccggcgtc cagagcccag atcgcgcgcg
     7681 ggaccggact ctccaagccg accgtgtcgc aggcgctgca gacgctcgag cgggcacacc
     7741 tggtccggga ggccgggcga tcctccggtg cgaagggccc gacggcgttg ctgtatgagc
     7801 tgaacccccg ggcgggctgg gtggtcggga tcgacgtcgg gcgggatctc gttcatgcgg
     7861 tgctcgccga tctgaacggc gacatagtgg ctcggcgcga cgaacgcgcc cacgcgcgca
     7921 gcgccggcgc cttgatcgcg cagatcggtg agctcgccca tgggctcgcg gccgacgccg
     7981 acttgaggtg gcggcaggtg acccaagccg cgatcggcag ccccggggtg ttcgatccgc
     8041 tccgagacca ggtcgtcctc gcccacaact tgccgggatg gggtcgtccg ggactcgtgg
     8101 aggcgctgca gaaggaactc ggcaccaaca tcgcgttcga gaacgacgtg aacctcgcgg
     8161 ccgtgggcga gcagacgcat ggcctgggca aggacgtccc gaacttcgcc tacctgcacg
     8221 tcggcaccgg cgtcggcctc gggcttgtcc tgaacggaga gctgtatcga ggctcgaggg
     8281 gagccgccgg cgaggtcgcc tacctcccac tcacgacgac cgacccgcac gagccgggga
     8341 gccgtcgacg cggggcgctc gagaccgcac tcggaacggc cgggatcgtg gccacggcgc
     8401 aacggctcgg gatgacgtcg tcggtgacgc cgcaacgggt gtttgcggcg gctcggcgcg
     8461 gggaccggac ggcgggccgg gtcacctccg cggtggccga acggatcgcc ctaacgatcg
     8521 cagccatcgt gccgatcgtc gacccagagc tcgtggtgct cggcggagcg atcgcgggga
     8581 acggcgatct gctgctcgac gcggtggacc gcgagctcca cgagatctcg cccttccgtc
     8641 cgcgggtcga ggtctccatg ctgggcgagg atgcggtgat ccgcggcgcg gtcgcggttg
     8701 cgcttcaggc ggcgcaggag cagctcttct cgaggaccgc acgtacatga aggaaggatc
     8761 gggtccgcaa ttgatcagtt cggagagggg gcgccgatga gacggatctt tttggcggct
     8821 tgcgcggccg tgctgttcgc ggccgcatgt accgcgggcg gtgggaccag cagcagcccg
     8881 accgtcgtcc acacgatcga cccgaacgta tcccatcagc cggtgacgct ggtggtctgg
     8941 gactggttcc ggcagcgcga gctcgagaac ctccagcaga tcacggccgc gttcgagcaa
     9001 cagtatccgt ggatcacgct tcaattcgtt ccaggcaaac atacgggcga gtacgtccgg
     9061 gcgatcaacg ccgacgtccc gatcgacgtg gcgatcgact ggggtccaga caatatcggg
     9121 aagtactgct ccaccggcgc ctgggtcgat ctcgcgccct acatcaaggc ctatggcctc
     9181 gatatggacg cgttgttccc cgggtccgcc ctgacctaca cgcggtacgg cggcgttcag
     9241 tgctcgctcc cggtcctcac ggacgcctac gggctgtact acaacaagga catgttcgcg
     9301 gaggccggga tccccgggcc gccgaagacc ctctcggagc tgacggagga tgccaagaag
     9361 ctcaccgagc tcaatcccga cggctcgatc aaagtcgctg gatttgtgcc catctcgacg
     9421 ttctacgaga acccaaacct ctacgacggg aacccgtggg gagcccagtg gtatcaggac
     9481 gacggctcgt cggctttcgc cagcgatccg gcgtgggccg agatggcgca gtgggacaag
     9541 gagctcaccg actggtacgg gtacgacaag ctccagacgt tctacgagga catcggtggg
     9601 ccggactcgg agtggtcgtc ggcgcacgcc ttcgagcagg gcaagatcgc gatgcactac
     9661 gacggcgagt ggcgcgtgtc gttcatcgac aacgacaacg cgagcgtcaa ctacggaacc
     9721 gcgccgttcc cggtcgccga cgacaagccg gatctgtacg ggtccgggca ggtgggtggg
     9781 acgatcatcg gcatccctcg caccgccgag catcccgatg aggcatggct gttggtccag
     9841 tggctctcga cgcagaccaa gcctctgaac cagctcgccg atctcctgaa gaacgtcccc
     9901 acgacgaagg agtcgcttca gacgacggcc ctgggagaag acccgcagtt cgagccgttc
     9961 cttgggatct accaggatcc tgcgtcgtac cactacccgc tgaagccgag cgggtccgcc
    10021 gacgccgacg cgctcgccgc gttcctcgaa gactgggagg cgggcgacgt cgccgacctg
    10081 cagcaggggc tgcaggatct agccgagcac gtggacgcac agcagcagct cggatgagcg
    10141 ctcccgcgat cgaggccgcg ggggcgcggg gcggcgtctc gttgccccga cggcgccgga
    10201 gacgtcccct ccgtgctcgc ttggccccgg cggtcttcat gtcgccgtgg gtggttgggt
    10261 tcgtcatttt catcctgtat ccgatgttgg cgacgctgta cttctcgttc acgaagtaca
    10321 acctgctcag ccctccacag tgggtcggct tgttcaacta ccgcttcatg tttacgagcg
    10381 atccgaactt ctggcaggcg gtgcggaaca cactctggat catcgctgta ggcaccacct
    10441 ccgggatcgt gttcgcgatc gcggcggcga tggtgctgac acgcgttcgg gcgggctcgg
    10501 gtgtgtaccg tacggtgttc ttcgtcccga cgatggttcc tgcggtggcg ggagcgctgg
    10561 cgttcgtctt cctgctccac ccgtcggggc ccgtcaactc gatcctcggg ctcttgcacc
    10621 tgccgcagcc gctgtggttt cgcgacccga cgtggtcgaa gccggcgctc gtcctcctgg
    10681 gcttgtgggg gatcggcgac acgatgatca tcttcctggc ggcgatgctc gacgtgccca
    10741 tccatctgta cgaggcggcg gacatcgagg gtgcgggcgc ctggcagaag tttcgccaca
    10801 tcacgctccc gatgatctcc ccggtcatct tcttcgccgt ggtgatcggg atcatcaccg
    10861 gcttccagta tttcgcgcag gcgtacgtcg catcgggcgg gacggatctg acgctcgggc
    10921 agccgcaggg atcgctgatg ttttactcga tctggcttta caaccagggg ttcgagtacg
    10981 gcaacatggg gtacgcggcg gcgatggcgt gggtcttgtt cgtcatcgtg atggcctgca
    11041 ccctcatctt gatccgaacc tcacgccggt gggtccatta ccaggggggg ttccgatagc
    11101 caccgccacc gcgtcgccgc tctcgatcgc cgacatccgc cctcgggcga tcccacccgc
    11161 cgcgcgtcgg cggcggttcc ttgtcgcggt cgcgaaccac tcgatcgcga tcgccctcgg
    11221 caccgtgttc gtgctcccct tcgtcttcgt tctcctgacc gcgctgatgg cgcgatcgca
    11281 ggccctctcg gggaccctgt ggccccggcc cttcgtcctc gacaacttcg tccgcgtctt
    11341 ccggttcatc ccgctctggc aatacacatg gaacacggtc ctgtatgcgg ggctctcgac
    11401 gctcgggacg gttgcatcat cgatcccggt cgcttacgcg ctgtcgcgga tgcgctggcg
    11461 cggccggcag gtcgcgtttg tcctcgtcct ctcgaccctc atgctgccgc cgcaggtgac
    11521 gatcgtgccg ctctacgtga tcttcgtgcg gatcggctgg attgggtcgc tgaagccgct
    11581 catcgttccg gcgtttttcg gcgacgcgtt cacgatcttc ttgttgcgtc agttcttcat
    11641 gacgattccc gaggagctgt cggacgccgc gagggtggac ggggccagcg agttccagat
    11701 cctgcgacgc gtgatcatcc cgctcgccaa accggcaatc gcggcggtcg cgctgttcca
    11761 gttcctctac tgctggaacg acttcttcgg cccgctgctc tacacgggaa gcaacccgaa
    11821 gatctggact ctcgcggtcg cgctttcgga gttctcctcg gtcctgcacc agggtgtgct
    11881 gtggaacatt cagatggccg cgtccgtcct attcatgctg cccgtgatcg ctctgttctt
    11941 cgtggcacag cgcgctttca tcgagggcgt gacgctgacc ggcgttaagg gctgaccggc
    12001 gttaagggct gaccggcgtt aggggctgac cggtgttagg ggctgaccgg cgttaggggc
    12061 tgaccggggt cttagggttc gatgaggtcc ataggagcca ctttttcccc taggaatggt
    12121 ctcagggacg tggcccctca ccgagcttcc ggtcggatat cggcgtcggg tgtcggctcg
    12181 tggcggccag gcgttatccc gcgcactcag tcggccatga cccatctatg tttgtcaagc
    12241 cgcctgctcg agccttctgc cgtcgatcac gatgtgccgg tagacaacgt tcgaaaggtg
    12301 gcgcttgagg catcgcatgg cctccgtgtg gggactttcc tgcctcccgc tggcgagcca
    12361 ggtaggtcct tcgcgtcggg ggaggtgcgg gagtgcgcgg acgatgcgag gctctgagcc
    12421 cgcgagcacg ccctcgcgtt gaccgccacg cgtcggacgc tacaccggcg tcatccgcgt
    12481 gagaagtcga acgacaacct ccccttcttc gaggaggcgc ccacggcgat ggctccagaa
    12541 cgttgccgcg tgtcgcgtca cctttcgtaa acttctgggc cgcgagccac cgatagagcg
    12601 actccgttca agggaaaagg ccccgtgacc aggggttttg ttggtgggcg ctagaggatt
    12661 tgaacctctg acctcttccg cgtcaaggaa gcgctctacc cctgagctaa gcgcccgaag
    12721 tccggcatgg taccgccggg ccgaggcggg gaccggaatt gaaccggtgt acagggtttt
    12781 gcagaccctt gcctaaccac tcggccaccc cg
//
LOCUS       DATBRK010000200         7049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_612081, whole genome shotgun sequence.
ACCESSION   DATBRK010000200 DATBRK010000000
VERSION     DATBRK010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612081"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="VIH70_06060"
     CDS             <1..1594
                     /locus_tag="VIH70_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8115959.1"
                     /translation="RVLLIGWDGADWRILEPLLEQGALPNLQALIDRGQKGVLRSTVP
                     THSWAAWPSFLTGVDPADHGVYDILETVPGTHKQYPVTYRSIKERTFVEDLSAAGKVG
                     VYADVPLLFPPPGINGKIAAGGVLPKGRNYTQPADLAEQLEKAGAPWVINGMSWTTYR
                     NRPEQYLDEALEITGKRIRATEWMMDNSDWDLMMSVWVSVDRTQHALSNYVAPDHPDY
                     AKNHTTPIGWKVRDVYAQLDDAIGSFVSRTRDDDLVLFISDHGFQSVTRTINMDQLLK
                     RFGFLEFSASNVVFGPMQWGPVRKVARKAYDFLGLHGKVSLPQSVNWSKTKAFTTIRS
                     TGEGVSINVAGREPDGIVDPADFEKVRDQVMDALSSFVDPKTGRKPVKAIYRREEIFK
                     GKHADTAPDILMEPAETYSLTHAKSALEDADWISGDHRPEGVIVAAGPNVKPFEQPPM
                     LIDMAPTILAALDAPASIEHTGRMLHEVVGSDARVGKRAEEPAKPVVQIPGMPSGSEE
                     SNVSDTEADEMEEHLRGLGYLE"
     gene            1684..1809
                     /locus_tag="VIH70_06065"
     CDS             1684..1809
                     /locus_tag="VIH70_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115960.1"
                     /translation="MLAPGMDAAIWTALGLLAATSLGSVFYLGSRIDCLGTVSMR"
     gene            2048..2905
                     /locus_tag="VIH70_06070"
     CDS             2048..2905
                     /locus_tag="VIH70_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115961.1"
                     /translation="MKRITIRVLVAVTLSMASVALGPAAQAVDTTILDTLGAATPNTT
                     FSVFGSGGLATFSSQLVGPRFVVNEDTVITEIGAFLNNCASIVGGVPQCPDALPLTVQ
                     IRPSVAGLPDPSTVLATLVLSHDDDPLLISYESVNPNLELAPGEYFALFAPQQEQDVG
                     FLLASTNEYRAGIVTMGFLDPTSGNASASDQFAAVRIVGHSLDASDLLNELLSDVIEQ
                     DAGRGANLESILSETGRALEAGNATSACQLLDAFVRATTAQSGKSLLPDQAAALIDAA
                     ADIQEALDC"
     gene            complement(2917..6087)
                     /locus_tag="VIH70_06075"
     CDS             complement(2917..6087)
                     /locus_tag="VIH70_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8115962.1"
                     /translation="MADTELPVDPPGHGRMSDVQIRTFLIADVRGYTLFTQERGDEAA
                     AKLAAKFADIAREIVEARGGALLELRGDEALCVFASTREAIRAAVDLQQRFVEETLEQ
                     PELPLTVGIGLDAGEAVPVQGGYRGAALNLAARLCGQARAGEILATREVTHLARRIED
                     ARYVDHGSLTFKGIADPVVVVRVVPEGEDPAVRLRPYAPAPPAPPTRPSRRWVAIGAI
                     AAVLALVAISIPLLTSGDGAEVDIGTNSVARMNAEDGAVQLTGELGQRPGASAIGFGS
                     LWVAQPDRGVVARLALDTGSVTDTIRVGNAPSGVAVGEGAVWVTNAGDGTVSRVDPDT
                     NEESQRLKAGSSPTGIAVGDGVLWVADSVGGALLRVDPTTQEVVEVALAGLPSGVTFT
                     PDGVWVSVAPNSVARIDPDAQNVVFAQPVGNGPTALVAAFGSIWVANHLDGTVTRLEP
                     STGRAEATIPVGQGPNTLAAAGGSVWVGNEFENTVVAIDPTTDDVEQTVPVGSAAASL
                     STDEEGLWLAVGASATEHRGGTLTVSAEDPAPKSLDPARVYDSEGWRILTITNDGLLA
                     YKKIGGPDGGTLVPDLASALPDVSPDGLTYRFPLRGGIAYSTGEPIRPEDYRHAIERT
                     LLLHPAGDLLSAIEGAAACLDEAALECDLSRGIETNEEAVTFHLATPDADLPFKLALP
                     FTFPVPSDIPAEDQGREPLPATGPYIVTDAGPKRVEMERNTTFEEWSAAAQPDGFVDA
                     ISWRFNEQLHGAFSRLQAGDLDVMVSEPGPQDLAVLRSTHPDQVLTWPGPFTLFVGFD
                     TLKPPFDDERVRRAVSFALDRGRIVDLLGGSTTQRPTCQILPPNFQGYEPYCPYTLEP
                     DNLVWSAPDPDRAKALIDEAGAAGTPVDVLVSDGLLPGSLDVMKYVSDVLGEIGLEPE
                     LEIVHGANAYFPQILPPAGGDPGTFAGTPRHPNVFLSGWLPDFPGARNFIEPQFACGE
                     AGYANGHGWCDETLSGRMDEASALQLTDPGAANRAWAEIEHQLVDAAVQAPITNPLTT
                     HAVSDRVENVQVHLQWGLMLSLIWVQ"
     gene            6213..6587
                     /locus_tag="VIH70_06080"
     CDS             6213..6587
                     /locus_tag="VIH70_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015339055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8115963.1"
                     /translation="MSEPSTPAVRVGSVVIDCNDFERMFAFWREAIGYVPRDEPEPDW
                     VVLRDPSGAAVNVSLQVVPEPRVGKNRLHLDLYTNDRDAEVDRLLGLGATRHARTPES
                     DEDFIVLADPEGNLFCVIQKDG"
     gene            6644..>7049
                     /locus_tag="VIH70_06085"
     CDS             6644..>7049
                     /locus_tag="VIH70_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115964.1"
                     /translation="MAEQQHAGRWPARITIGLIIGAVEAIFAMAFAALVFGGYLADFI
                     ADGISLYLGAAALTLGIFAWLAGPRGVVGGLQPATAVVLATVTATVAVTAFGSIHRAF
                     LTVVATGLVVTALGGIAFTMLGTTRRANLIRFV"
BASE COUNT         1122 a         2420 c         2403 g         1104 t
ORIGIN      
        1 acgagtactg ctcatcggat gggatggcgc cgactggcgc atcctcgaac ccctgctcga
       61 gcagggcgcc ctgccaaacc tccaggccct gatcgatcgg gggcagaagg gcgtcctgcg
      121 ctcgacggta ccgacccact cgtgggcggc ctggccgtcg ttcctgacgg gagtcgaccc
      181 ggccgatcac ggggtctacg acatcctcga gaccgttccc gggacccaca agcagtaccc
      241 ggtcacctac cggtctatca aggagcggac gttcgtcgag gacctctcgg cggccggcaa
      301 ggtcggggtg tacgccgacg tgccgctcct gttcccaccg ccgggcatca acgggaagat
      361 cgccgccggg ggcgtgctgc cgaaggggcg caactacacg cagccggccg acctggccga
      421 gcagctcgag aaggccggcg cgccgtgggt gatcaacggc atgtcctgga ccacctaccg
      481 gaaccggccc gagcagtacc tggacgaggc gctcgagatc accggcaagc ggatccgcgc
      541 gaccgagtgg atgatggaca actcggactg ggacctgatg atgtcggtgt gggtctcggt
      601 cgaccggacg cagcacgcgc tctcgaacta cgtcgccccc gaccaccccg actacgcgaa
      661 gaaccacacg acgccgatcg gctggaaggt tcgagacgtc tacgcgcagc tcgacgacgc
      721 gatcggctcg ttcgtctctc gtacgcgcga cgacgacctc gtcctgttca tctcggacca
      781 cgggttccag tcggtcacac ggacgatcaa tatggaccag ctgctgaagc ggttcggctt
      841 cctcgagttc agcgcgtcga acgtggtctt cgggccgatg cagtgggggc cggtgcgcaa
      901 ggtcgcgcgc aaggcctacg acttcctcgg cctgcacggg aaggtctcgc tgccgcaatc
      961 ggtgaactgg tccaagacca aggcgttcac gacgatccgc tcgaccggcg agggcgtctc
     1021 gatcaacgtg gccgggcgcg agcccgacgg catcgtcgac cccgccgact tcgagaaggt
     1081 gcgcgaccag gtgatggacg cgctctcgag cttcgtcgac cccaagaccg ggcgcaagcc
     1141 cgtcaaggcg atctaccgac gcgaggagat cttcaagggc aagcacgccg acacggcgcc
     1201 cgacatcctg atggagccgg ccgagacata ctcgctcacg cacgccaagt ccgcgctcga
     1261 ggacgccgac tggatcagcg gcgaccaccg gcccgagggc gtgatcgtgg cggccggccc
     1321 gaacgtgaag ccgttcgagc agccgccgat gctgatcgac atggcgccga cgatcctggc
     1381 ggcgctcgac gcgccggcct cgatcgagca caccggccgc atgctgcacg aggtcgtcgg
     1441 ctcggacgcg agagtgggga agcgggcaga ggagccggcg aagcctgtcg tgcagatccc
     1501 cggcatgccg agcggctcgg aggagtcgaa cgtcagcgac accgaggcgg acgagatgga
     1561 ggagcacctc cgcggtctcg gctacctcga gtagtcggga gttctcgccg gaccggccga
     1621 taccgatttc tgtatcgaca gcgctcgaga ggcgcgaacc ttccgcgtca ccgagtggta
     1681 tttatgctcg cgccggggat ggacgccgcg atctggaccg ccctcggctt gcttgccgcc
     1741 acgtcgctgg gaagcgtgtt ctatctcggc tctcggatcg actgcctcgg aaccgtgtcg
     1801 atgcgttagg cgcaggcctg gatacgagac tcgatgcgat cgagtctcct tgatgcacac
     1861 ctggaacatc acactgccgt ttagccgttc gcgttctctc cgcctcggcc acccaaatag
     1921 ctgtcgccag tggccacctg catggggcgc gcccgcaagg tcgcgtagtt ctacgggttc
     1981 ccctaccgat ccggccgcct accgtcgagc cgtcatcccg ggcaggcatc gagaaggagg
     2041 tagggagatg aagcggataa cgatccgtgt gcttgtggcg gttacgttga gtatggcgag
     2101 tgtcgcgctg ggacccgcgg ctcaggccgt cgatacgacg atcctggaca cgctcggagc
     2161 agccacgccc aacaccacat tcagcgtctt cggaagcggc ggactggcga ccttcagttc
     2221 ccaactcgtg ggtccgaggt tcgtagtgaa cgaggatacg gtcatcaccg agatcggcgc
     2281 cttcctcaac aactgcgcat cgatcgtcgg tggcgttccc caatgcccag acgcgctgcc
     2341 gctgacggtg cagatccgtc cctcggttgc tggacttccc gatccgtcga cggtgctggc
     2401 gacgctcgtg ctctctcacg acgacgatcc actcctgatc tcgtacgagt cggtgaatcc
     2461 gaacctggag ctggcgcccg gcgagtactt cgccctcttc gctcctcagc aggagcagga
     2521 cgtcggtttc ctgctggcga gcacgaacga gtatcgcgcc ggcatagtca cgatgggatt
     2581 cctcgaccca acgagtggga acgcgtccgc gtccgaccag ttcgctgccg tacgaatcgt
     2641 cggccattcg ctggatgcca gcgatctgct gaacgagttg ctgagcgatg tcatcgaaca
     2701 ggacgcaggt cgtggagcca atctcgaaag catcctgagc gagaccggcc gtgcactcga
     2761 agcagggaat gcgacgtccg catgccagct cctggacgcg ttcgtccggg caacgacggc
     2821 gcaatcgggc aagagcttgc tgcccgacca ggctgccgcg ctgatcgatg cggccgccga
     2881 catccaggag gcgctggatt gctaggcgac agtgatctac tgcacccaga tcaggctcag
     2941 catgagcccc cactgcaggt ggacctggac gttctccaca cgatccgaca cggcgtgggt
     3001 cgtgagaggg ttggtgatcg gggcctgcac cgctgcgtcg accaactggt gctcgatctc
     3061 tgcccacgcg cggttcgccg cgccggggtc ggtcaactgc aacgcgctcg cctcgtccat
     3121 ccgtccgctg agcgtttcgt cgcaccatcc gtgcccgttg gcgtacccag cctcgccgca
     3181 cgcgaactgg ggctcgatga agttacgagc cccggggaag tccgggagcc acccgctcag
     3241 gaagacgttc gggtgtcgcg gcgtccccgc gaacgtgccg ggatcgccac ccgccggcgg
     3301 taggatctgg gggaagtacg cgttagcccc gtgcacgatc tcgagttccg gctcgaggcc
     3361 gatctccccc aggacgtcac tgacgtactt catgacgtcc agcgaaccgg gaagcaatcc
     3421 gtcgctgacc aggacgtcga ctggggttcc cgccgctccg gcctcatcga tcagggcctt
     3481 cgcccggtcg gggtccgggg ccgaccagac gaggttgtcg ggttcgagcg tgtaggggca
     3541 gtagggctcg tagccctgga agttcggcgg caggatctgg caggtcgggc gctgggtcgt
     3601 cgagccgccg agcaggtcga cgatccgccc gcggtcgagt gcgaagctga cggcccggcg
     3661 cacgcgctcg tcgtcgaacg gcggcttcag ggtgtcgaac cccacgaaca gcgtgaacgg
     3721 tcccggccag gtgaggacct gatcggggtg ggtcgaccgc aagacggcca ggtcttgcgg
     3781 tcccggctca ctgaccatca cgtcgaggtc gccggcctgg agtcgtgaga acgccccgtg
     3841 aagttgctcg ttgaaccgcc atgagatcgc gtcgacgaac ccgtcgggct gcgccgcggc
     3901 cgaccactcc tcgaacgtcg tgttccgttc catctcgacg cgtttcggcc ccgcgtcggt
     3961 gacgatgtac gggccggtcg cggggagagg ttcgcggcct tgatcctcgg ccgggatgtc
     4021 cgacgggacc gggaaggtga acggcagcgc gagcttgaac ggaaggtccg cgtccggcgt
     4081 cgcgaggtgg aacgtgacgg cctcctcgtt cgtctcgatc ccccgggaca gatcgcactc
     4141 gagagctgcc tcgtccaggc aggcggccgc cccctcgatc gcgctcagca ggtccccggc
     4201 cgggtgcagg agcagcgtcc gctcgatcgc atggcggtag tcctcgggcc tgatgggctc
     4261 gcccgtcgaa tacgcgatcc cccctcgcag cgggaatcgg taggtcagac cgtcgggcga
     4321 gacgtcgggc agtgccgagg ccaggtccgg gaccagcgtg ccgccgtcgg gtccaccgat
     4381 cttcttgtac gccagcagtc cgtcgttggt gatcgtcagg atccgccaac cctcggagtc
     4441 gtagaccctc gccgggtcga gggacttggg cgccggatcc tccgcagaga ccgtcaacgt
     4501 tccgccccga tgctcggtcg ccgaggcacc cacggccaac cacagaccct cctcgtcggt
     4561 ggagagcgag gccgccgcgg agccgaccgg aacggtctgc tcgacgtcat cggtggtcgg
     4621 gtcgatcgcg acgacggtgt tctcgaattc gttgccgacc cagaccgacc cgcccgccgc
     4681 ggccagcgtg ttcgggccct gaccgaccgg gatcgtcgcc tcggctcgtc ccgtcgacgg
     4741 ctcgagccgc gtcaccgtgc cgtccaggtg gttcgcgacc catatcgagc cgaaggccgc
     4801 gaccaatgct gtggggccat tgccgaccgg ttgggcgaag acgacgttct gcgcgtcggg
     4861 atcgatgcgg gccacgctgt tcggcgcgac cgagacccac accccatcgg gcgtgaacgt
     4921 cacaccggaa ggaaggccgg cgagggcgac ctcgacgacc tcttgggtgg tcggatcgac
     4981 ccggaggagt gcgccgccga ccgagtccgc cacccacagg acgccgtccc ccaccgcgat
     5041 cccggtcggg gacgagcctg ccttcagtcg ctggctctcc tcgttggtgt cgggatcgac
     5101 ccggctcacg gtgccgtccc ctgcgttcgt cacccacacc gccccctcgc cgacagcgac
     5161 gcccgaggga gcgttcccga cccggatcgt gtccgtgacc gagccggtgt cgagcgccag
     5221 gcgggccacg accccgcggt cgggctgggc cacccagagg cttccgaaac cgatcgcgct
     5281 cgcccctgga cgctggccga gctcacccgt gagctggacg gcgccgtcct ccgcgttcat
     5341 ccgcgcgacc gaattcgtcc cgatgtccac ctcggcgccg tcgcccgagg tgagcagcgg
     5401 gatcgagatc gccacgagtg cgagcaccgc ggcgatcgcg ccgatcgcaa cccaacgccg
     5461 ggaaggtctg gtgggcggcg ccggtggagc gggcgcgtac ggccgaaggc gtactgccgg
     5521 gtcctcgcct tcggggacga cgcggaccac cacgaccggg tcggcgatgc ccttgaaggt
     5581 cagcgaaccg tgatcgacgt atcgggcgtc ctcgatccgg cgcgcgagat gcgtgacctc
     5641 gcgagtggcg aggatctccc cggcgcgagc ctggccgcag agccgggccg cgaggttcag
     5701 cgcggcaccc cggtagcccc cctggaccgg gacggcctct cccgcgtcga gcccgatccc
     5761 caccgtgaga gggagctccg gctgctcgag ggtctcttcc acgaagcgct gctggaggtc
     5821 gacggcggcg cggatcgcct cgcgggtcga ggcgaacacg cagagcgcct cgtctccccg
     5881 aagctcgagg agcgctccgc cgcgcgcctc gacgatctcg cgtgcgatgt cggcgaactt
     5941 cgcggcgagc ttcgccgctg cctcgtcgcc ccgctcctgg gtgaacaggg tgtacccgcg
     6001 gacgtccgcg atcaggaacg tccggatctg gacgtcgctc atacgaccgt gaccgggcgg
     6061 gtcaaccggg agttccgtgt ctgccacgcg cggagtatgc gctgcggccg ttccgaacga
     6121 ccaccgccgc tcgagccgaa gacgcgagat ccccctggag cgacgcacca ccggacgacg
     6181 ccgcaacaag cagcctcggg tagggtcgtg ccgtgagcga gccatcgacg ccggcggtgc
     6241 gggtcggctc ggtcgtcatc gattgcaacg acttcgagcg gatgttcgcg ttctggcggg
     6301 aagccatcgg ctacgtccct cgggacgagc cggagccgga ttgggtcgtg ctccgggatc
     6361 catcgggcgc ggctgtgaac gtctcgctgc aggtcgtgcc cgagccgagg gtcgggaaga
     6421 accggctcca cctcgatctc tacacgaacg atcgagacgc cgaggtcgac cggctcctcg
     6481 ggctgggagc gacccggcat gcgaggaccc cagagtcgga cgaggacttc atcgtgctcg
     6541 ccgaccccga gggcaacctc ttctgcgtca tccagaagga cggctgaacc gggccgcgcg
     6601 cgccggctcg ccgttcggcc tacagtccgc acgggaggga gccgtggccg agcagcaaca
     6661 cgcgggccga tggccggccc ggatcaccat cgggctgatc atcggcgccg tcgaggcgat
     6721 cttcgcgatg gcgttcgccg ccctcgtgtt cggcggctac ctcgcggact tcatcgccga
     6781 cggcatcagc ttgtacctgg gggcggcggc gctgacgctc gggatcttcg cctggctcgc
     6841 gggccccagg ggggtggtcg gcggacttca gccggccacg gcggtcgtgc tggcgaccgt
     6901 gacggcgacg gtcgcggtca ccgcgttcgg gagcatccac cgggccttcc tcaccgtcgt
     6961 cgccaccggc ctggtcgtga cggccctggg cgggatcgcc ttcacgatgc tcgggaccac
     7021 ccggcgcgcg aacctcatcc ggttcgtcc
//
LOCUS       DATBRK010000201         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_468143, whole genome shotgun sequence.
ACCESSION   DATBRK010000201 DATBRK010000000
VERSION     DATBRK010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468143"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1934
                     /locus_tag="VIH70_06090"
     CDS             <1..>1934
                     /locus_tag="VIH70_06090"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY8115965.1"
                     /translation="LIEDEDERFHILESLRAATTGAGSRVRIVATLRADFFDQPLSVR
                     GFGDLLAQRTEAITPMSPEELERAIVAPAERTGLAVEPRLLAAMIADVVDRPGALPLL
                     QFTLTELAERQKDGVLTLVGYRRIGGVSGALARRAEQLFEAMNERARDVCRQVFLRLV
                     TLGEGSEDTRRRVRRSELQPLTDARTMDGVLDAFGRHRLLSFDRDPATREPTVEIAHE
                     ALLGAWDRLSGWIDEARDDVRTQRRLAAAVSEWESAERDPSYLLRGARLDQVHEWVGT
                     TTLALSDDDRAYVAEGVEVQRAVFEREHAMERRSVRRLRSLVALGVAAALVATTLTVV
                     AMNQRGRAEEGARVSRARELAAAAVANLDVDAERSILLAMEAVDATWDVDRTVVPQAE
                     EALHRALQESRVVLTVPQGREIAVSADGSRFATTGQDGTATVWETDTGKRLLTLRGHE
                     GEVNGVAFNPDGSLLATTGDDRTVRLWDAASGRQIHVLRGHRDLVLGVAFSPDGSLLA
                     TSSQDGTVRIWDVAAGTQDLVLRGPPGEQFANYSGLTPAFSPDGSRVASGGWFSTPIW
                     DLATGKISMLLARQPVEAFAVAFSPDGTRVATAVHRDAQTWDAETGEPLTAFSGHTGD
                     ILAVAYSPDGTRIATGADDG"
BASE COUNT          293 a          662 c          708 g          271 t
ORIGIN      
        1 ccctgatcga ggatgaggac gagcggttcc acatcttgga aagcctgcga gcggcgacca
       61 caggggccgg cagcagggtc aggatcgtcg cgacgctccg cgcggacttc ttcgaccagc
      121 cgctctcggt gcgtggtttc ggcgatctac tcgcccaacg caccgaggcg atcacgccga
      181 tgtctcccga agagctcgag cgggcgatcg tcgcccccgc ggagcgaacg gggcttgcgg
      241 tcgagccgcg actgctcgcc gcgatgatcg cagacgtcgt ggaccgtccg ggagcgctcc
      301 cgctgctcca gttcacgctc accgagctcg ccgagcggca gaaggacggc gtgctcacgc
      361 tcgtcggcta ccgacggatc ggcggcgtgt cgggcgcgct ggcacgccgc gccgagcaac
      421 tgttcgaggc gatgaacgag cgtgcacggg acgtgtgccg acaggtgttc ctccggctgg
      481 tcacgctggg tgaggggagc gaggacacgc gacgccgcgt ccgccgatcg gagctgcagc
      541 cgctcacgga cgcccggacg atggacggcg tgctcgacgc gttcggccgg catcgcctgc
      601 tctcgttcga ccgtgatccc gcgacccgcg agccgaccgt cgagatcgcc cacgaggcgc
      661 tgctgggagc gtgggatcgg ctgagcggtt ggatcgacga agcccgagac gatgtccgca
      721 cgcagcgccg gctcgcggcg gccgtctccg aatgggaatc ggccgaacgc gacccgagct
      781 acctgctccg gggggcccgg ctcgaccagg tccacgagtg ggtgggcacc acgacgctgg
      841 ccctctcgga cgacgaccgg gcctacgtcg ccgagggcgt ggaggtgcag cgggcagtct
      901 tcgagcgcga gcacgcgatg gagagacgat cggtgcggag gctgcgctcg ctcgtggcgc
      961 tcggggtcgc cgcggcgctc gtcgcaacga ccctgaccgt ggtcgcgatg aaccagcgcg
     1021 gacgagcgga ggagggagcg agggtgtctc gggcgcgtga gctcgcggcc gcggcggtcg
     1081 ccaacctgga cgtcgacgcc gagcgaagca tcctgttggc gatggaggcc gtcgacgcca
     1141 cgtgggacgt cgatcgtacg gtcgttcctc aagccgagga ggctcttcac cgggccctac
     1201 aggagtcccg agtcgtcctc accgtgccgc aagggcgcga gatcgccgta agcgccgatg
     1261 gcagccggtt cgccaccaca ggccaggacg ggaccgcaac cgtgtgggag acggacaccg
     1321 gcaagcgcct ccttaccctc cgcgggcacg agggtgaggt caacggcgtc gccttcaacc
     1381 ccgacgggtc tctgttggcc accaccggcg acgatcggac ggtgcgcttg tgggatgcgg
     1441 ccagcggccg gcagatccac gtgctgcgag ggcatcggga tctggtcttg ggcgtcgcgt
     1501 tcagccccga cgggtcgctg ctagccacat ccagccagga cggcaccgtt cggatctggg
     1561 acgtcgcggc gggcacgcag gacctcgttc tgaggggtcc ccccggcgag cagttcgcga
     1621 actactcagg cctcacacct gcctttagcc ccgacggttc ccgggtggcg agcggggggt
     1681 ggttcagcac gcccatctgg gacctcgcga cggggaagat ctcgatgctc cttgcccgcc
     1741 agccagttga ggcctttgcg gtcgcgttca gccccgacgg cacacgcgtc gcgaccgccg
     1801 tccaccgtga cgcgcagacg tgggacgcgg agaccgggga gcccctcacc gcgttctccg
     1861 gccacacggg ggacatcctc gcggtcgcct acagccccga cggcacgcgg atcgctaccg
     1921 gcgcggacga cggg
//
LOCUS       DATBRK010000202         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_177893, whole genome shotgun sequence.
ACCESSION   DATBRK010000202 DATBRK010000000
VERSION     DATBRK010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177893"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1730)
                     /gene="topA"
                     /locus_tag="VIH70_06095"
     CDS             complement(<1..>1730)
                     /gene="topA"
                     /locus_tag="VIH70_06095"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY8115966.1"
                     /translation="LPKSDLGVDVDNGFEVSYEVPEDSKKHVAALKKALKANEDLILA
                     TDYDREGEAIAFHVASLLGVDPAQAKRVTFTEITRDAILEAFRRPREIDLKLFDAQEA
                     RRVLDRLVGYKISPLLWRRVQPGLSAGRVQSVALRMIVERERQIRGFTPAEYWSVDAR
                     LTPRGEDRPFLARLVSVPEGRIASAPDKKGVLLTAEAQAAAHVDRLREADYRVAKVES
                     KERKEGPRPPFKTSTLQQEAARKLGFGARKTMTLAQRLYEGVDLPGEGTVGLITYMRT
                     DSLSLAEAALREIGELVRTEFGPEYALAEPRRFKTRSRNAQEAHEAIRPTSALRTPDR
                     VAAALDRDQLRLYTLIWQRTVATQIAQARFNQVGVDIDATAGTARYRLRATGRTMIFD
                     GHLRVYAEGRDDGADEDGERRLPELSAEQALSLLELLPEQHFTQPPPRFSEASLVKTL
                     EELGIGRPSTYASIISTIQDRGYVRLEDRRFFPNDVGEVVTDKLVEHFPDIVNVNFTA
                     EMEGDLDEIAEGDLQRVQVLRQFYTPFADALERAEEKFERYVEELDEDCPRCIEEGRT
                     PPGRLQKRLGR"
BASE COUNT          232 a          613 c          605 g          280 t
ORIGIN      
        1 cgcccgaggc gcttctgcaa ccggccgggc ggggtgcgtc cctcctcgat gcaacgtggg
       61 cagtcctcgt cgagctcctc cacgtaacgc tcgaacttct cctccgcgcg ctcgagggcg
      121 tcggcgaacg gcgtgtagaa ctgacggagc acctgcacgc gttgcaggtc gccctcggcg
      181 atctcgtcga ggtcaccctc catctcggca gtgaagttca cgttgacgat gtcggggaag
      241 tgctcgacga gcttgtcggt cacgacctcg ccgacgtcgt tggggaagaa ccggcggtcc
      301 tcgagccgga cgtaaccgcg gtcctggatc gtcgagatga tcgacgcgta ggtcgacggc
      361 ctgccgatac ccagctcttc gagcgtcttg acgagggacg cctcggagaa gcgcgggggc
      421 ggctgggtga agtgctgctc gggcaggagc tcgaggagcg acagcgcctg ctccgccgag
      481 agctcgggga gccggcgctc gccgtcctcg tcggccccgt cgtcccggcc ctcggcgtac
      541 acgcggagat gcccgtcgaa gatcatcgtg cgcccggtcg ctcgcaaccg gtagcgcgcg
      601 gtgcccgccg tcgcatcgat gtcgacgccc acctggttga agcgggcctg cgctatctgc
      661 gtggcgacag tgcgctgcca gatcagggtg tacagccgca gctggtcccg atccagggcg
      721 gcggccaccc ggtcgggcgt ccgcagcgcc gacgtcggcc ggatcgcctc gtgcgcctcc
      781 tgcgcgttcc gcgaccgggt cttgaaccgg cgcggctcgg ccagcgcgta ctcggggccg
      841 aactcggtcc gcacgagctc gccgatctcg cgcagggccg cctcggccag gctgagcgag
      901 tcggtccgca tgtacgtgat cagaccgacc gtgccctcgc cgggcaggtc cactccttca
      961 tagaggcgct gcgcgagcgt catcgtcttc cgggcgccga acccgagctt gcgggccgcc
     1021 tcctgctgca gcgtggaggt cttgaacggg ggccgaggcc cctccttgcg ttccttgctc
     1081 tcgaccttgg ccacgcggta gtctgcctcg cggagccggt cgacatgggc cgcggcctgg
     1141 gcctcggcgg tcagcagcac gcccttcttg tcgggcgcgc tcgcgatccg cccctcgggc
     1201 acgctcacga gccgggcgag gaagggacga tcttcgcccc gcggggtgag gcgcgcatcg
     1261 acgctccagt actcggccgg ggtgaagccg cggatctggc gctcgcgctc gacgatcatc
     1321 cgcagcgcga ccgactgcac gcgccctgcc gagagcccgg gctggacccg ccgccagagc
     1381 aggggcgaga tcttgtagcc gaccagccgg tcgagcacgc gccgcgcctc ctgggcatcg
     1441 aacagcttca ggtcgatctc gcgcgggcgg cggaaggcct ccaggatcgc gtcgcgcgtg
     1501 atctcggtga acgtgacgcg cttggcctgc gccggatcga cccccagcag ggacgccacg
     1561 tggaacgcga tcgcctcgcc ctcccggtcg tagtccgtcg cgaggatcag gtcctcgttc
     1621 gccttcaggg ccttcttcag cgcggcgacg tgcttcttcg aatcctcggg gacctcgtag
     1681 gagacctcga acccgttgtc gacgtcgacg cccaggtcgc tcttggggag
//
LOCUS       DATBRK010000203         6947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_468239, whole genome shotgun sequence.
ACCESSION   DATBRK010000203 DATBRK010000000
VERSION     DATBRK010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468239"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..4262
                     /locus_tag="VIH70_06100"
     CDS             33..4262
                     /locus_tag="VIH70_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8115967.1"
                     /translation="MIEGLHPAVRTWFGRRFPDGPTLPQAGGWAEIAAGRHALIAAPT
                     GSGKTLAAFLVCIDHLYRAHEAGAVLGEGPQVVYVSPLKALAVDIHQNLEAPLREIAE
                     VAAELGLDAPNIRVAVRTGDTAASERAAMLRRPPNFLVTTPESLYLLLTAERSRGMLE
                     GVDTVILDEIHAVAGNKRGSHLALTLERLHHVAGTPPQRIGLSATQRPIDAIARLLVG
                     AGPDRSNPDGSPACAIVDVGHQRALDLAIELPDDELGAVASTEQMGEIIDRITEHVKG
                     HRTTLVFVNTRRMAERIAHQMGERLGEDQVAAHHGSLSKERRRRVETRLRAGELRALV
                     ATASLELGIDIGPVELVCQIGSPRSLATFLQRVGRSNHTRFGTPRGVLYPTSRDELVE
                     CAALLRGTREGLLDAIVQPVAPLDIVAQQIVAECSAEEWSEDALFDLIHRAAPFARIS
                     RRDFEDVVELVSEGVTTGRGRRAAYLHRDRVNRRLKGRRGARLAALTSGGAIPELGDY
                     RVVAEPDDIFVGTVNEDWATESMAGDVFLLGTTSWRIRRVEPGTVRVVDAQGAPPSVP
                     FWLGEAPGRTKELSDEVSELRSKVGARLERGDPDGARGWLEDKCGIDQAASDTIVRYL
                     GAGLTALGALPTKQLLVLERFFDESGGMQLVGHSPFGARLNRGLGLALRKRFCVTFDF
                     ELQAAASDDAILLSLGPQHSFPLERVPKFLASKTVEDVLRQAVLTSPMFAARWRWNLN
                     RALTVLRMRGGRKNPPPIQRMESDDLMAAVFPSLAACQENVAPGPIEIPDHPLVRQTL
                     DDCLHEAMDVDGLRQLLSEIESGRVEVVVRDTTEPSVLTHEILNGRPFTYLDDAPLEE
                     RRSRAVQLRRGLPVEARDLDRLDPAAIERVRDEVQPDPRDADELHDLLMTVSGLRPEE
                     KWRSWFDELVEERRAVTVRAAPDVDLWCTVERRPAMELMFPAAAIVPDHRSPIAVAPL
                     DRDAAEAEMVRGHLEYQGPSTVADLADVTGLPEGDVAIAVARVEGEGFAFRGHFTSTD
                     GPDEFCARRLLARIHSYTQQRLRREIEPVTARDFMRFLLRWQHVAPGTQRQGRLGVLA
                     VVEQLQGFELAAGAWEPDILASRVEGYRAERLDDLCLSGDVTWGRLSLRAAEAEEIHR
                     RSGMPPSRATRVTLTRRQDLPWLLQAARGDRKPQAPGPGRTEDALEALRRHGALFPSD
                     LITITGRLPTEVAEALWDAVARGFVTCDGFDAVRSLLHRRTVARAPRRSGLRRAGTST
                     TARSAGRWSLVPGTQATEDPDELAEAVAEQLLARWGVVFRDLLGRETFTVPWREVLWA
                     LRRMEARGTIRGGRFVTGFSGEQYALPEAVDSLRAVRKHERTGESVTISAADPLNMVG
                     ILLPGPRVPALMKNAVSYVDGVPEDAAPAFA"
     gene            4535..4948
                     /locus_tag="VIH70_06105"
     CDS             4535..4948
                     /locus_tag="VIH70_06105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019747211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEY8115968.1"
                     /translation="MATFSPAELDYLTSERRLGRLATADADGHLHVVPVGMWRVNSDL
                     GTIDITGHDFTRKRKFANVRVNPRAALVVDDLASTDPWRPRSVMVEGPAQAIDASPET
                     GGEGIIRIEPETIVSWGLEADDMRSTSGRATSDDP"
     gene            complement(5492..6355)
                     /locus_tag="VIH70_06110"
     CDS             complement(5492..6355)
                     /locus_tag="VIH70_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8115969.1"
                     /translation="MDIGIGIPNTVLDTPGALLIEWSRRAEARGFVSLATIGRIAYPA
                     HDELIGLTAAAGATERIGLFTNILLVPAFPVAVLAKQTATLASASRGRFTLGAGVGTR
                     PDDFAASASSFQDRGRRFDRQLEALHAAWRGDPLPGTDHRVAQPVPNGRVPMMFAGQP
                     RFAAPRAVRWNAGYTIGGAAPEHAAGAAEEFRRTYADLDGAGVPRVAALAYFSLGDEH
                     TEESLRNLRTYYALLGDWAETIAQGAARSPHDLRSRMRAYEDAGIDELLFDPTVANID
                     QVDRLADIVFG"
     gene            complement(6645..>6947)
                     /locus_tag="VIH70_06115"
     CDS             complement(6645..>6947)
                     /locus_tag="VIH70_06115"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEY8115970.1"
                     /translation="VEGQPETRDLIVVPGRVVRNWWRRDGHALVLEDLEDVLDELPER
                     LIVGMGGSSQMRPDPAALERLSERGIEVECLPTDRAVIRFGELDPARTAVALHLTC"
BASE COUNT         1082 a         2367 c         2400 g         1098 t
ORIGIN      
        1 tcgctgtcgg tttgggaaag tagggtgacg gggtgatcga aggactccat ccggctgttc
       61 ggacgtggtt cggcaggcgg tttcccgacg gcccgacctt gccacaagcc ggcgggtggg
      121 ccgagatcgc cgctggccgg cacgccctga tcgcggctcc caccggctcg ggcaagacgc
      181 ttgctgcctt cctggtctgc atcgaccacc tgtatcgggc gcacgaggcc ggcgccgtcc
      241 tgggcgaagg accacaggtc gtctacgtct cgccgctgaa ggcccttgcg gtcgacatcc
      301 accagaatct ggaagccccg ctgcgggaga tcgccgaggt cgcggccgag ctcggactcg
      361 acgccccgaa catccgggtg gcggttcgga ccggggacac ggccgcctct gagcgggccg
      421 cgatgcttcg gcggccgccg aacttcctgg tcaccacccc tgaatctctc tacctgctgc
      481 tcacggcgga gcgctcgcgg gggatgctcg aaggcgtcga caccgtgatc ctcgacgaga
      541 tccacgccgt ggccgggaac aaacgaggat cacacctcgc gctcacgctg gaacgactcc
      601 atcacgtcgc tgggacgccg ccccagcgga tcgggctgtc ggccacacag cgcccgatcg
      661 acgccatcgc tcggctgctc gtcggcgcgg gcccggaccg gtcgaacccc gacggatcgc
      721 cagcgtgcgc gatcgtcgac gtcgggcacc agcgcgctct cgatctggcg atcgagcttc
      781 ccgacgacga gctgggagcc gtggcgtcga ccgagcagat gggggagatc atcgaccgaa
      841 tcaccgagca cgtgaagggc caccgcacga cgctcgtctt cgtcaacacc cggcggatgg
      901 ccgagcgcat cgcgcaccag atgggagagc ggctcggcga ggatcaggtc gccgcccacc
      961 acggcagcct gtcaaaggag cgccgccggc gtgtcgagac gcgtcttcgg gccggcgagc
     1021 ttcgggccct cgtggcgacc gcgtcgctgg agctcgggat cgacatcgga ccggtggagc
     1081 tcgtctgcca gatcggctcg ccgcggagcc tcgccacgtt cctccagcgc gtgggccggt
     1141 ccaaccacac ccgcttcggc acacctcggg gcgtgctcta cccgacgagc cgcgacgagc
     1201 tcgtcgagtg tgcggcgctc cttcgcggta cccgagaggg actcctggac gcgatcgttc
     1261 agcccgtcgc gccgctcgac atcgtggctc agcagatcgt ggccgaatgc tccgccgagg
     1321 agtggtccga ggacgcactg ttcgacctga tccatcgggc cgctcccttc gcgcggatct
     1381 cccgcaggga cttcgaggac gtggtcgagc tcgtctcaga gggcgtcacg acaggccgtg
     1441 gacggcgagc cgcgtacctg caccgcgatc gagtgaatag gcgcctgaag ggccgccggg
     1501 gtgcgcggct ggccgcgctc acctcgggcg gcgccattcc cgagctgggc gactaccgcg
     1561 tggtggccga gcccgacgac atcttcgttg ggacagtcaa tgaggactgg gccaccgaga
     1621 gcatggccgg cgacgtgttc ctcttgggga cgacctcgtg gcggatccgc cgcgtcgagc
     1681 ccggcacggt tcgcgtagtc gatgcgcagg gcgcgccacc gtcggtgcct ttctggctgg
     1741 gtgaggcgcc cgggcgaacc aaggagctct ccgacgaagt gtccgagctc cgctcgaagg
     1801 tcggggcacg tctcgagcgc ggcgaccccg acggtgcgcg cggatggctt gaagacaaat
     1861 gcgggatcga ccaggccgcc tccgacacca tcgtccggta tctgggtgcg ggcctgaccg
     1921 ccctcggcgc gcttcccacg aagcagctcc tcgtcctgga gcgattcttc gacgagagcg
     1981 gcgggatgca gctcgtgggg cattccccgt tcggggcgcg gctgaaccgg gggctcgggc
     2041 tcgcccttcg caagcggttc tgcgtgacct tcgacttcga gctgcaggcc gcggccagcg
     2101 atgacgccat cttgttgtcc cttggtcccc agcacagctt cccgctggag cgggtgccga
     2161 agttcctcgc gtcgaagacg gtggaggatg tgctccggca ggccgtgctg acctcgccca
     2221 tgttcgcggc gcggtggcgc tggaacctga accgagcgct taccgtgctc cggatgcggg
     2281 gcggtcgtaa gaatccgccg cccatccagc ggatggagtc cgacgacctg atggcggcgg
     2341 tgttcccgag cctcgcggca tgccaagaga acgttgcgcc cggcccaata gagatcccgg
     2401 accaccccct cgtgcgccag acactcgacg actgcctgca cgaggcgatg gacgtcgacg
     2461 gcctccgcca attgctgtcc gagatcgagt cgggccgggt cgaagtggtt gtgcgagaca
     2521 cgacggagcc ttcggtcctg acccacgaga tcctcaacgg gcggcccttc acctacctcg
     2581 acgacgctcc cctggaggag cgccgcagca gggcggtgca gctccggcgc gggctgccgg
     2641 tggaggcccg cgacctagac cggctcgacc ccgcggcgat cgagcgggtc cgcgatgagg
     2701 tccagcccga cccgcgcgac gccgacgagc tccacgacct gctgatgacg gtctcggggc
     2761 tgcgcccaga ggagaagtgg cggtcctggt tcgacgagct cgtcgaggag cgccgggcgg
     2821 tcacggtgcg cgccgctccg gacgtggacc tgtggtgcac ggtcgaacgg cgacccgcca
     2881 tggagctcat gttcccggcg gcggcgatcg tgcctgacca tcgctcgccg atcgctgtcg
     2941 caccgcttga ccgggacgcc gccgaagcgg agatggtcag aggacacctc gagtaccaag
     3001 gaccatcgac cgtggccgat cttgcggacg tgaccgggct gcccgagggt gatgtcgcga
     3061 tcgcggtcgc ccgggtggag ggtgaaggct tcgcattccg aggccacttc acatcgactg
     3121 acggaccgga cgagttctgc gcacggcggc tgctcgcccg gatccacagc tacacgcagc
     3181 agcgtctccg acgcgagatc gagcccgtca cggccaggga cttcatgcga ttcctcctgc
     3241 ggtggcagca cgtcgcgccc gggacccagc ggcaggggcg cctgggcgtc ttggcggtcg
     3301 tcgagcagct ccagggcttc gagcttgccg cgggggcgtg ggaaccagac atcctcgcct
     3361 cgcgcgtgga gggatatcgc gccgaacggc tcgacgacct gtgcctgtcc ggcgacgtca
     3421 cgtggggccg actctcgctt cgggccgccg aggcagagga gatccaccgg cgaagcggca
     3481 tgcccccctc cagggcgacc cgggtcacgc tgaccaggcg gcaggacctg ccgtggctcc
     3541 tgcaggcggc gcgaggcgat cggaagccgc aggcgcctgg gccgggccgg acggaggacg
     3601 ccctcgaagc cctccgacga catggggccc tgttcccttc cgacctgatc acgatcaccg
     3661 gacggctgcc caccgaggtc gcggaagcgt tgtgggacgc ggtcgcacgg ggattcgtca
     3721 cctgcgatgg cttcgatgcg gtccgctcgc tcctgcatcg acggaccgtg gctcgggccc
     3781 cgcgccgctc cgggctcagg agggccggca catccaccac cgccagatcc gccgggcgct
     3841 ggtcgcttgt gcccggaacg caagccaccg aggatcccga cgagctggcc gaggccgtcg
     3901 ccgagcagct cctcgcacgg tggggtgtgg tgttccggga cctgctgggc cgcgagacct
     3961 tcacggtccc gtggcgggaa gtgctatggg cgcttcgccg tatggaggct cgggggacga
     4021 tcaggggagg aaggttcgtc accggcttca gcggcgagca gtacgccctt cccgaggcgg
     4081 tggactccct gcgagcggtg cggaagcacg aacgcaccgg cgagagcgtg acgatctcgg
     4141 ccgccgaccc cctgaacatg gtggggatcc tgttgcccgg cccacgcgta ccggcgctca
     4201 tgaagaacgc ggtcagctac gtcgacggcg ttccagaaga tgccgcgccc gcgttcgcct
     4261 aaggtccatc caacgatcat cggaagacct ctccgatcac ggagccgttg aaccggacca
     4321 tgctccgcgc atgactcctt ttgggatcca ggtcgtgatt gaagatggtg atgtcagacc
     4381 gcggctcata tagcctgagg cgtggtctta tttccggagg cggcctgagc atctcacggg
     4441 agattctcgc ttcagatgac cggtaggcag ggattcatcg gccccgatcg ccggttgtac
     4501 gtgattgatc gtacgcgagc aggaggtcgg accgatggcc acgttctctc cggcagagct
     4561 tgactacctg acgagcgagc gacggctcgg acgcctcgcc accgccgacg cggacggcca
     4621 tctccacgtc gttccggtcg gaatgtggcg cgtgaacagc gacctcggaa cgatcgacat
     4681 caccggccac gacttcacgc gcaagcgtaa gttcgccaac gtccgcgtca atccacgagc
     4741 ggcgctcgtc gtcgacgacc tggcgagcac cgacccgtgg cgaccgcggt cggtcatggt
     4801 cgagggcccg gcgcaggcga tcgacgcctc gcccgagacc ggcggggaag gaatcatccg
     4861 catcgagccc gagacgatcg tctcatgggg actcgaggcg gacgatatga gatcgacgtc
     4921 gggccgcgcg acatcggacg atccctaacg cctcggccta gtttcggcga aggaggtctg
     4981 cggcgtgaat agcggtatcg cagatcctgg cgagtgattt ccaatagagg ttgcagtgag
     5041 gtagacgtct ggctcagcgt ccacacgacg acacgtacga cgagctcgtt ggggaggtga
     5101 cgcgtcatgg ctgacttgcc gttcgaggag cactccgagg aactgtgggc gtttccggat
     5161 gtcgacggtc cgatcaattc ccgaccgccc acggagtggt tctctagatc gaagtccctt
     5221 ctgccggcgt gccgtcactg ccgcgagtcg gtgggctcct cgacgtggtc gtggtgttcg
     5281 cgtccgcgtg gttcgaccgg ccgatgacgg gtcggggcgt cgacatgagg tggccggacg
     5341 tgggcttcta tctggacagg agcttgtccg ccggtgtcct tgtctgctca cccggcttcc
     5401 aacccctcct tcgggtccga gacgcgaccg cacaacctga agccccgact gaacgtcatg
     5461 acgaggctgg cgagcatttc actcgccagc cttaaccgaa gacaatgtcg gcgagtcgat
     5521 ccacctgatc gatgttcgcc acggtcgggt cgaacaggag ctcgtcgatc ccggcgtcct
     5581 cgtatgccct catgcgactg cgcagatcgt gggggcttcg cgccgctcct tgcgcgatgg
     5641 tctcggccca gtccccgagc aaggcgtagt acgtccgaag gttccgcagc gactcctcgg
     5701 tgtgctcgtc gcccaacgag aagtacgcga gcgcggcgac gcgggggacg ccggcgccgt
     5761 cgaggtcagc gtacgtccgt cggaactcct cggccgcgcc ggccgcgtgc tcgggtgcag
     5821 ctcccccgat cgtgtagccg gcgttccatc gaacggctcg cggagcggcg aagcgcggct
     5881 ggcccgcgaa catcatcggt acccgtccgt tcggcaccgg ttgagcgacg cggtggtcgg
     5941 tccccgggag cgggtcgcct cgccacgcgg cgtgcagcgc ctcgagctgc cgatcgaagc
     6001 gacggccgcg gtcctggaac gaggacgctg aagccgcgaa atcatcgggt cgggttccga
     6061 cgcctgcccc gagcgtgaac cggccccgac tcgccgatgc gagcgtggcg gtctgcttcg
     6121 cgaggacggc tacggggaac gccggcacga gcaggatgtt cgtgaacagc ccgatgcgct
     6181 ctgtggcgcc cgcggcggcg gtcagcccga tgagctcgtc atgtgccggg taggcgatcc
     6241 gcccgatcgt cgcgagggac acgaatccac gtgcctcggc gcgacgagac cactcgatca
     6301 ggagtgctcc gggcgtgtcc agcacggtgt tcgggatccc gatgccgatg tccactgcca
     6361 cccccccttc cgcccgttcg tcttcacagc gacgcgagca gcctccccac aggctacggc
     6421 cgtcgaccga gcccgaggca cgcatatgac cgatccggtt aggaccttga acttccgtcc
     6481 gtcggagttc acgtccttcg cccttcggcc gctggcgcgc tcatccaaat cgggagcacg
     6541 gcgaggtcat ggagccaaac cggcgggtcg gcgatcgggg ttcgtaagga tgaccgggcc
     6601 cggtcttcgc ccgcgtcgtc agcgcgactg gaccggcgcc atgttcagca cgtgaggtgc
     6661 aatgcgacgg cggtccgggc ggggtcgagc tcgccgaacc tgatcacggc gcgatctgtt
     6721 gggaggcact ccacctcgat cccgcgctcg ctcagacgct ccagcgcggc agggtcgggg
     6781 cgcatttgcg acgacccccc catccccacg atcagccgct cggggagctc atcaagaacg
     6841 tcctcgagat cctcgagcac cagcgcgtgc ccgtcgcgac gccaccagtt ccgtacgacc
     6901 cggccaggca ccacgatcag gtcgcgcgtc tcaggttgtc cttccac
//
LOCUS       DATBRK010000204         9886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2489208, whole genome shotgun sequence.
ACCESSION   DATBRK010000204 DATBRK010000000
VERSION     DATBRK010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489208"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..507)
                     /locus_tag="VIH70_06120"
     CDS             complement(160..507)
                     /locus_tag="VIH70_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HEY8115971.1"
                     /translation="MEVNDVISQARDTLTVKRVFGEPYEKNGVTVIPAASVQGGAGGG
                     GGEGPDGQGKGSGSGFGLNARPVGAFLIRGDEVMWRPAVDLNKVVLGAQVVAVVALLT
                     IRTIVKARAKSRA"
     gene            541..684
                     /locus_tag="VIH70_06125"
     CDS             541..684
                     /locus_tag="VIH70_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115972.1"
                     /translation="MHLRERFPQSDPGDEQDGERGIWIQSARPDRAPVLVAEGDFVTW
                     SPV"
     gene            851..1387
                     /locus_tag="VIH70_06130"
     CDS             851..1387
                     /locus_tag="VIH70_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY8115973.1"
                     /translation="MRHPRHPHHHDSPFGRHWDKGWFGGPPWGGGHRARRGDVRTALL
                     SLLSDKPMHGYDLIRELEQRSGGAWRPSPGSIYPTLQLLEDEGLVTSEEQDGKRVYTI
                     TDAGRAELEERRERGGGDPWEFGQLGEGLGQLREAGVQLAAAAMQVGRTGSEAQRKHA
                     AEILAEARKKIYGLLAED"
     gene            1431..1946
                     /locus_tag="VIH70_06135"
     CDS             1431..1946
                     /locus_tag="VIH70_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY8115974.1"
                     /translation="METWDALRARRNVRSYEDRAIPDEDLERILEAARRTPSSMNQQA
                     WDFVVVTDRDLLGELAQTWRYAAHVGASVATIVLVVPTSVDRDERDTLQYDIGQVTMS
                     IMLAAADLGIGSGHASVGDQDLARAILGVPEDRECAILIALGFPAGRPLQPMNRPNRR
                     AIEDVVHRERW"
     gene            1954..2838
                     /gene="ligD"
                     /locus_tag="VIH70_06140"
     CDS             1954..2838
                     /gene="ligD"
                     /locus_tag="VIH70_06140"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY8115975.1"
                     /translation="MTTARVEVSRPDKLLWPSIGVTKQAYVDYLGAVADRMLPWLRDR
                     PLSLVRAPDGVDGQRYFQKNTPSHAPSWIKTVTIPAPSAGRDVSYTVCNDADTLAWLG
                     NQAALEFHPAPVRRDKLDRPDLFVVDIDPPDDAFDAAVEVALLVLEVFDDLGLETLLK
                     TTGGKGLHVVVPIVRHVSAERLRHAATRLAEIVTDRRPDLVTAEFRKAKRAGRVMLDP
                     SRNGPGATLVAPYSPRARAEGTVSFPVLPRDLRKIAPADFTLASVGGRLVNGAAPKRW
                     SEAANGPHQRLPASLLSE"
     gene            2857..3135
                     /locus_tag="VIH70_06145"
     CDS             2857..3135
                     /locus_tag="VIH70_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8115976.1"
                     /translation="MEDEELLARAEQALTEIKVGQGLSDRQASVLAALRIRLKGSADK
                     SLEEMLEAAGDDLQPDRLEQALEKAEGEPKTTLDDLLSKPKPKPEWPS"
     gene            complement(3146..4543)
                     /locus_tag="VIH70_06150"
     CDS             complement(3146..4543)
                     /locus_tag="VIH70_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY8115977.1"
                     /translation="MTDRVSSGSERLDAVLGGGLVANSINVVMGLPGSGKTILAQQYL
                     FENASEDRPAVYLSTVSEPLEKILRYGQTLSFFDPSAIGGSVIYEDLGESLTHQDALT
                     ETLGRIVAILRERRPGLLVIDSFKALRPFAADATVFRRFLQDLAGTLTAYPATSLWVG
                     EYGPDEIAVAPEFAVADGIISLAVEPTADRDTRVLQVQKLRGTGFLSGKHWYRLSADG
                     IRVFPRLADPVDQAAYTLETERVSSGIPVLDDALDSGLWPGGSTLVAGPSGSGKTLIG
                     LHYIFKGAEIGRPGLIAGLQENPTQLERITNGFGWSLDRDDVHVMYRSPVDMYLDEWV
                     HELLEQVEEHGIRRIMIDSLGDLRATATDEIRFREYVYSLLQRTAHAGIGVFMTQEVA
                     ELFGVTRLSEYGISHLSDNVVLLQFLRGESQIKRALTVLKTRASAHDPHILEFEITPN
                     GIELGKRFADDQSLA"
     gene            complement(4540..5184)
                     /locus_tag="VIH70_06155"
     CDS             complement(4540..5184)
                     /locus_tag="VIH70_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8115978.1"
                     /translation="MPEVATYSIGAVARMVRVPPATLRTWEERYAIVRPVRSAGGHRL
                     YSRMDVERLRFVADRVREGISPGDAHRMLAGVSGNLPTAAREPGTRIVILLAERDPYA
                     AEFAEFFLKTEGFEVVLALDADDAVGAIAADRPQLAIVDLLISGGAGLELCRRVHDRG
                     IPVLALSSVATRDAALEAGASAFLEKPLEPLQLVSVVKDLLGTSSFLNEVVPAR"
     gene            5388..5747
                     /locus_tag="VIH70_06160"
     CDS             5388..5747
                     /locus_tag="VIH70_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8115979.1"
                     /translation="MQLLSIEQGESIRLAGELDMSTASQLREVLDVAVAHGGTILVDV
                     SEVTFMDSTGIGAFLRAAVSLRGRGCIILHGEQDRVRRVLDLVRVDGSIPNLHRVTHD
                     FALDSQNGSYSGHSQVS"
     gene            complement(5744..6142)
                     /locus_tag="VIH70_06165"
     CDS             complement(5744..6142)
                     /locus_tag="VIH70_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8115980.1"
                     /translation="MGPANECELRLRPESEAPSLARAAIAAAASGVPAVVVADAELLT
                     SELVSNAVRHAELDPSQEIVVRIVSDGYVRVEVADQGPPFEADLRKPSAGSSGWGLLL
                     VEAVATSWGVEREGSGKRVWFEVGARRSGR"
     gene            complement(6215..6817)
                     /locus_tag="VIH70_06170"
     CDS             complement(6215..6817)
                     /locus_tag="VIH70_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8115981.1"
                     /translation="MRRLMVTTFLTLDGVMQGPGGPGEDDSGGFTLGGWSVTYWDDRM
                     REFMNQTMSVPFDLLLGRRTYEIFKGYWPNASEEAGSKPLNDATKYVVSRGRPTLDWK
                     ESVLIEGDAAEGIASLKATDGPELQVHGSGNLIQTLLRHSLVDEFRLWAFPVVIGSGK
                     RLFADGAVPAGLKLVDNVVSTTGVVIGTYEPAGELVTGSF"
     gene            complement(6895..8814)
                     /locus_tag="VIH70_06175"
     CDS             complement(6895..8814)
                     /locus_tag="VIH70_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEY8115982.1"
                     /translation="MPDLESVLAGLNEAQREAAFALRGPVAILAGAGTGKTTTITHRI
                     ASQVRSGAFEPSQILAVTFTDKAAGELRARLGALGVEGVEARTFHSAALSQLSRLWSA
                     YTGEPLPEVLDHKAPLISSLANALPPPHKFLPRRELAGEIEWAKNRMIPPERYLDELD
                     DHEPPIPAELMLRVYDGYERRKRSMGRLDFEDMLGLALRLFDEHPESVQQVRSRFAAF
                     TVDEYQDVNPLQAALLERWLGARDDLCVVGDDYQTIYAFTGASPSHLLGFTTRFPHAT
                     VVRLEQNYRSTPEVLELANRLATRLGGFRKTLRSTRPSGAPPLARAEPDEEAEVGDVV
                     QAVRRLHREEDVPLEEIAVLYRINARSEPFEEAFAAAAIPYQVRDGAFLRRPGPRAVL
                     QRLKRSGAGSVAEAVEAITGQLGYDPQATPDADEEVTRQSDLGRMRSLAVEFERAGGG
                     DAAAFGAELAKRFSTEHSGRGVNLLTYHRAKGLEFDAVFLPRLLDGELPFRSGRAKAD
                     PEEERRLLYVGITRARRYLFLTWPSAGRARPSPFLDEMELAQSAPSRARAAPGPAVTV
                     GRGGALFDRLKEWRRKRANADGVPAYVVFHDRTLAEIAERRCTDWADLAAISGVGPAK
                     LERYADEVLAVVAAG"
     gene            9030..>9886
                     /locus_tag="VIH70_06180"
     CDS             9030..>9886
                     /locus_tag="VIH70_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY8115983.1"
                     /translation="MTEHAKSEPSGDRGRPAAPPPPPAWRNWLLVVGLVVSVLILFWP
                     RGTRSVSELSYTEFLSRVKAGEVATAVIDPGGAVSGTLENGGDYTTQIPIALQDPGLA
                     EELRANGVEITGKAEPGATLLSVILSFLPFLLLIGVWLYIGRRTQRQLAGGIGGILGS
                     RAKVYDAEAPSTRFNDVAGYEGAKQEISEVVDFLKNPQRYAKAGAMGPRGVLMVGPPG
                     TGKTLLARAVAGEAGVPFLAITGSGFVELFVGVGASRVRDLFTDAKKRAPAIVFIDEV
                     DAIGQRRGAG"
BASE COUNT         1533 a         3498 c         3384 g         1471 t
ORIGIN      
        1 gcccgcgaag tacggacccc cggggtggcc gagcagcacc tcgaggcgac cccctcgcga
       61 tcgccacaac aggatgccgg cgctggtgcg gcccgacttg tcgaccttcg gctttcggac
      121 gcccgccatc tctggttctc cggagccgaa gaggcgcgct caggccctgg acttcgcgcg
      181 agccttgacg atcgtccgga tcgtgagcag ggccacgacg gcgacgacct gggctccgag
      241 gacgaccttg ttgagatcga cggcgggccg ccacatcacc tcgtcgcccc tgatgaggaa
      301 cgccccgaca gggcgggcgt tgaggccgaa accgcttccc gagcccttgc cctggccatc
      361 ggggccctcc ccgcctcctc ctccggcgcc accctgaacg ctggcggccg ggatcacggt
      421 cacgccgttc ttctcgtagg gttcgccgaa cacgcgcttg acggtgagcg tgtcccgggc
      481 ctgcgagatg acgtcgttga cctccatgac ggcctccttc ccgctgctcc gtcctccatc
      541 ttgcaccttc gcgagcgctt cccgcagtcg gatcccgggg atgagcagga cggcgagcgg
      601 gggatctgga tccagtcagc ccggcccgac cgggcgccgg tcctcgtcgc cgagggcgac
      661 ttcgtgacct ggtcacccgt ctgatcggta ccgagagttc ccgcgcgccg gcgatctcgt
      721 tttcggtatc gggcgccctc gggaagatct gatccacccg acggaccgcc cggtcgttcg
      781 gggatccaga aaggacgatg tgttacgata catcgcgacc cattgacgag aggtcgaaag
      841 gagcgagatc atgcgccatc cgagacaccc gcaccaccac gactcaccgt tcggtcggca
      901 ctgggacaag ggctggttcg gtggcccgcc gtggggcgga ggccaccgcg cgcggcgggg
      961 cgacgttcga acggcgttgc tctcgctgct ttcggacaaa ccgatgcacg gctacgacct
     1021 gatccgcgag ctcgagcagc ggagcggcgg ggcgtggagg ccgagcccgg gatcgatcta
     1081 ccccacgctg cagctgctcg aggacgaggg gctcgtgacg agcgaggagc aggacggcaa
     1141 gcgcgtctac acgatcacgg acgcgggccg agccgagctc gaggagcgcc gggagcgcgg
     1201 gggcggggac ccgtgggagt tcgggcagct cggcgagggg ctcgggcagc tccgcgaggc
     1261 cggggtccag ctcgcggccg cggccatgca ggtcgggcgg acgggctcgg aggctcagcg
     1321 caagcacgcc gccgagatcc tggccgaggc gcgcaagaag atctacggtc tgctcgccga
     1381 ggactgaccg cctggcgaac ccgccatcct ggagcgttat cgttcccggc atggagacct
     1441 gggacgcact ccgagcccgc cggaacgttc ggtcctacga ggaccgtgcg atccccgacg
     1501 aggacctcga acggatcctc gaggcggcgc gccgaacgcc ctcctcgatg aaccagcagg
     1561 catgggactt cgtcgtcgtc accgatcgcg atctgctcgg tgagctcgcg cagacatggc
     1621 ggtacgcggc ccacgtggga gcctccgtcg cgacgatcgt cctcgtcgtc ccgacctccg
     1681 tggaccgcga cgagcgcgac acgctccagt acgacatcgg acaggtcacg atgtcgatca
     1741 tgctcgccgc ggccgatctg gggatcggga gcgggcacgc ctcggtcggc gaccaggacc
     1801 tcgcccgtgc catcctcggt gtgcccgagg atcgggagtg cgcgatcctc atcgcgctcg
     1861 gtttccccgc cggccgtcca ctccaaccga tgaaccgtcc caaccgccgg gccatcgagg
     1921 acgtcgtcca ccgcgagcgc tggtagccgc gcgatgacga ccgctcgcgt cgaggtctcc
     1981 cgtccggaca agctcctgtg gccctcgatc ggcgtcacca agcaggcata cgtcgactac
     2041 ctcggcgccg tcgcggaccg gatgctcccg tggcttcgcg atcggcccct ctcgttggtt
     2101 cgcgctcccg atggcgtgga cggacagcgg tacttccaga agaacacgcc gtcgcacgcg
     2161 ccgtcctgga tcaagacggt cacgatcccg gcgccgagcg ctggccgcga cgtctcctac
     2221 accgtgtgca acgacgcgga cacgctcgcg tggctcggga accaggctgc gctcgagttc
     2281 cacccggcgc cggtccgccg agacaagctc gaccgtccgg acctgttcgt cgtggacatc
     2341 gacccacccg acgacgcgtt cgacgcggcg gtcgaggtcg cgctcctggt actcgaggtc
     2401 tttgacgacc tcgggctcga gacgctgctc aagaccaccg gcggaaaggg cctccacgtc
     2461 gtggtgccga tcgtccggca cgtgagcgcc gaacgcctcc gccacgccgc gaccaggctc
     2521 gccgagatcg tcaccgaccg ccggcccgac ctcgtgaccg ccgagttccg caaggcgaag
     2581 cgtgcggggc gcgtgatgct cgacccctcg cgcaacggcc cgggcgccac gctggtggcc
     2641 ccgtactcgc cccgagcccg agccgaaggc acggtctcgt tcccggtcct gcccagggac
     2701 ctgcggaaga tcgcgccggc cgacttcacg ctcgcgagcg tcggcgggcg gctcgtgaac
     2761 ggcgccgccc ccaagcgctg gtccgaggcg gcgaacggcc cccaccagcg cctgccggcc
     2821 tcgctcctct ccgagtaagc gtggcagact ggtcgggtgg aggacgaaga gctcctcgcc
     2881 cgggccgagc aggcgctgac cgagatcaag gtcggccagg gtctgtcgga ccggcaggcc
     2941 tcggtgctcg ccgcgctccg catccggctg aagggcagcg ccgacaagtc gctcgaggag
     3001 atgctcgagg ccgcgggtga cgacctgcag cccgatcgcc tcgaacaggc gctcgagaag
     3061 gccgagggcg aacccaagac cacgctcgac gacctgctga gcaagcccaa gccgaaaccc
     3121 gagtggccgt cctgaccggc gctggctagg ccaaggactg atcgtcggcg aaccgcttgc
     3181 cgagctcgat cccgttcggg gtgatctcga actcgaggat gtgcggatcg tgcgcgctcg
     3241 cgcgggtctt cagcacggtg agcgcccgct tgatctgcga ctcaccccgc aggaattgca
     3301 acaggacgac attgtccgac aggtgcgaga tcccgtactc ggacagacgc gtcacgccga
     3361 acagctcggc gacctcctgc gtcatgaaca cgccgatccc ggcgtgcgcg gtccgctgga
     3421 ggagcgagta cacgtactcg cggaaccgga tctcgtcggt cgcggtcgcc cgaaggtcgc
     3481 ccaggctatc gatcatgatc cggcggatcc cgtgctcctc gacctgttcc aggagctcgt
     3541 ggacccattc gtcgagatac atgtcgaccg ggctgcgata catgacgtgc acgtcgtcgc
     3601 ggtcgagcga ccagccgaac ccgttggtga tgcgctcgag ctgcgtgggg ttctcctgga
     3661 ggccggcgat caggccgggc cggccgatct ctgcgccttt gaagatgtag tgcaacccga
     3721 tcagggtctt gcccgagccg gacggacccg ccacgagcgt cgaccctccc ggccacaggc
     3781 cggaatccaa cgcatcgtcc aggacgggga tccccgatga gacgcgctcg gtctccaggg
     3841 tatacgcggc ctggtccacc gggtcggcca gcctcgggaa gacgcggatg ccgtcggccg
     3901 agagccggta ccagtgcttc ccgctcagga acccggtgcc tcggagcttc tgcacctgaa
     3961 ggacccgcgt gtcgcggtcg gccgtcggtt cgacggcgag cgagatgatc ccgtcggcga
     4021 ccgcgaactc gggcgccacc gcgatctcgt ccggtccgta ctcccccacc cacagcgacg
     4081 tggcggggta cgcggtcagg gtgccggcca ggtcctgcag gaatcgccga aacaccgtcg
     4141 cgtccgcggc gaacggacgc agcgccttga agctgtcgat cacgagcaac cccggtcgtc
     4201 gctctcgcag gatcgcgacg atgcgcccga gcgtctcggt cagcgcgtcc tggtgagtca
     4261 ggctctcccc caggtcctcg tagatcacgc tgccgccgat cgcgctcggg tcgaagaagg
     4321 acagggtctg tccgtagcgg aggatcttct cgagcggctc cgagaccgtc gagaggtaga
     4381 ccgcgggccg atcctcgctc gcgttctcga acaggtactg ctgcgccagg atcgtcttgc
     4441 ccgagccggg tagacccatg acgacgttga tcgagtttgc cacgaggccg ccgcccagga
     4501 cggcgtcgag ccgctcgctg ccgctcgaga cccggtcggt caccgcgcgg ggacgacctc
     4561 gttcaggaac gagctcgttc ccagcaggtc cttcacgacg gaaacgagtt gcagcggttc
     4621 gagcggtttc tccaggaacg cggacgcgcc ggcctcgagc gccgcatcgc gcgtcgcgac
     4681 gctcgacagc gccaggaccg ggatgcctcg gtcatggacc cgtcgacaca gctcgagccc
     4741 ggcgccgccg gagatcagga ggtcgacgat ggcgagctgc ggccggtcgg cggcgatcgc
     4801 gccgacggcg tcgtccgcgt cgagcgcgag cacgacctcg aaaccctcgg tcttcaggaa
     4861 gaactcggcg aactcggccg cgtaggggtc gcgctcggcg agcaggatca cgatccgggt
     4921 cccgggttcg cgagcggccg tcgggaggtt gccggacacc cccgcgagca tgcggtgcgc
     4981 gtcgcccggc gagatcccct cgcgcacgcg gtcggcgacg aagcggaggc gctcgacgtc
     5041 catccgcgag tacaggcggt ggccgccggc gcttcgaacc ggccgcacga tggcgtaccg
     5101 ctcctcccat gtacggaggg tggccggcgg aacccgcacc attcgcgcga cggccccgat
     5161 gctgtaggtg gcgacttcgg gcacgaccgg cccagcatac cccggccaac ctgcctagat
     5221 aaagaggagt tacggaacct tcataggagg cgaaaactgc gccaatatct cttcgaaatc
     5281 tggagctacg gcttgatccc cttgacccac gcctggaatc aggcataacc tcctcactag
     5341 ttatctcgcc ggagaacgcc cggagaacgc ggaggaaggg gtcgatcgtg cagctgcttt
     5401 cgatcgagca gggcgagagc atccgtctag ctggggagct ggacatgagc accgcgtccc
     5461 agctccgcga ggtcctcgat gtggccgttg cgcacggtgg gacgatcctg gtcgacgtca
     5521 gcgaagtcac gttcatggac tccacaggca tcggcgcctt cctgcgggct gcggtgtccc
     5581 tgcgtggacg cggatgcatc atcctgcacg gcgagcagga tcgcgttcgg cgggtgctgg
     5641 acctcgttcg ggtcgacgga tcgatcccga acctccaccg cgtgacccac gacttcgccc
     5701 tcgactcgca gaatggctct tactccgggc attcgcaggt cagctagcgc ccgctgcgac
     5761 gcgctcccac ctcgaaccag accctcttgc ccgaaccttc gcgttcgaca ccccacgacg
     5821 tcgcgaccgc ctcgaccagg agcaagcccc aaccgcttga tcccgcactg ggcttgcgca
     5881 gatcggcttc gaacggtggc ccttgatccg cgacctcgac gcggacgtag ccatccgaca
     5941 cgatccgcac gacgatctct tgcgagggat cgagctccgc gtgcctgacc gcgttcgaga
     6001 ccagctcgga cgtcaggagc tccgcatcgg ccaccaccac cgcgggcacg ccggatgcgg
     6061 cggcagcgat cgcggctcgc gcgaggctcg gcgcctccga ctccgggcgg agtcgtagct
     6121 cgcactcgtt cgcgggcccc atcgcgtgca acctcgcttc gatcccgaac atggttgtac
     6181 ccggtcgccc ggcggcaaac accccggtca gctgtcagaa cgatccggtg acgagctcac
     6241 ccgcgggctc ataggtgccg atgacgaccc ccgtggtgga gaccacgttg tcgacgagct
     6301 tcagcccggc tggaacggcg ccgtccgcga acaggcgctt gcccgacccg atgacgacgg
     6361 ggaacgccca caggcggaac tcgtcgacca gggagtggcg aaggagcgtc tggattaggt
     6421 tcccgctgcc gtggacctgg agctcgggcc cgtcggtcgc cttgagcgac gcgatgccct
     6481 cggcggcgtc accctcgatg agcaccgact ccttccagtc cagcgtgggg cgaccccgcg
     6541 acacgacgta cttcgtggcg tcgttcaacg gcttggagcc ggcctcctcg gatgcgttcg
     6601 gccagtagcc cttgaagatc tcgtaggtcc tgcgtccgag caggaggtcg aacggcacgc
     6661 tcatcgtctg gttcatgaac tcgcgcatcc gatcgtccca gtaggtcacc gaccagccac
     6721 cgagcgtgaa cccgccgctg tcgtcctcgc cgggtccgcc gggtccctgc atgaccccgt
     6781 cgagggtcag gaacgtggtg accatcagcc ttcgcatcgc tcgcgtcctt ccgatcgccc
     6841 gttccgttcg aggataggac cgctcggctc ggcgagaccg atcggcggtc ctcgttaccc
     6901 ggccgcgacg accgccagga cctcgtccgc gtagcgctcg agcttggccg gccccacgcc
     6961 cgagatcgcg gcaaggtcgg cccagtccgt gcagcggcgt tcggcgatct cggcgagcgt
     7021 gcggtcgtgg aacacgacgt acgccggcac gccgtccgcg ttcgcgcgct tgcggcgcca
     7081 ttccttcagc cggtcgaaca acgcgccgcc acggccgacc gtgaccgccg gaccgggcgc
     7141 cgcccttgcc ctcgagggcg ccgactgcgc gagctccatc tcgtcgagga acgggctcgg
     7201 ccgcgcgcgg ccggcgctcg gccaggtgag gaacaggtac cggcgggcgc gcgtgatccc
     7261 gacgtacagg agccgccgct cctcctcggg gtcggccttc gcgcggcccg atcggaacgg
     7321 cagctcgccg tcgagcagcc tcggcaggaa caccgcgtcg aactccaggc ccttcgcgcg
     7381 atggtacgtg agcaggttca cgccgcgccc gctgtgctcg gtcgagaacc gcttggcgag
     7441 ctcggcgccg aacgccgccg cgtcgccccc gccggcgcgc tcgaactcga ccgcgagcga
     7501 ccgcatccgg ccgaggtcgg actggcgagt gacctcctcg tcggcgtcgg gcgtggcctg
     7561 ggggtcgtag ccgagctggc cggtgatcgc ctcgacggcc tcggccaccg aacccgcgcc
     7621 ggaacgcttc agccgctgca ggacggcgcg cgggcccggc cggcgcagga acgcgccgtc
     7681 gcgcacctgg tacgggatcg ccgcggccgc gaacgcctcc tcgaacggct cggaacgagc
     7741 gttgatccga tacagcaccg cgatctcctc gagcggcacg tcctcctccc ggtggaggcg
     7801 ccggaccgcc tgaacgacgt cgccgacctc ggcctcctcg tcgggttcgg cgcgtgcgag
     7861 cggaggggct cccgatggcc gggtcgagcg aagcgtcttg cggaaccctc cgagtcgtgt
     7921 cgcgagccgg ttggcgagct cgaggacctc gggcgtcgag cgatagttct gctcgagccg
     7981 gacgacggtc gcgtgcggga accgggtcgt gaagccgagc aggtgcgacg gcgacgcgcc
     8041 ggtgaacgca tagatcgtct ggtagtcgtc gccgaccacg cacaggtcgt cgcgggcgcc
     8101 gagccagcgc tcgagcaggg cggcctgaag cgggttcacg tcctggtact cgtccacggt
     8161 gaacgcggcg aatcgcgaac ggacctgttg cacggactcg gggtgctcgt cgaacagccg
     8221 gagcgcgagc ccgagcatgt cctcgaagtc gagccgcccc atcgaacgct tgcgccgctc
     8281 gtagccgtcg tagacgcgaa gcatcagctc ggccgggatc ggcggctcgt ggtcgtcgag
     8341 ctcgtcgagg taccgctccg gcgggatcat ccggttcttc gcccactcga tctcgccggc
     8401 gagctcacgc cgagggagga acttgtgcgg tggcgggagc gcattcgcga gcgacgagat
     8461 gagcggagcc ttgtggtcga gcacctcggg gagcggctcg ccggtgtatg cggaccacag
     8521 ccgcgagagc tgcgacagcg cggccgaatg gaacgtgcgc gcctccacgc cctcgacgcc
     8581 gagcgcaccg agccgagccc gcagctcccc cgcggccttg tccgtgaagg tcaccgcgag
     8641 gatctgggaa ggctcgaacg caccgcttct cacctggctg gcgatccgat gcgtgatcgt
     8701 ggtggtcttg cccgtccccg cgccggccaa gatcgcgacc ggccctcgga gcgcgaaggc
     8761 cgcctcacgc tgggcctcgt tcaggccggc gaggacgctt tcgagatcgg gcacagctcg
     8821 gaccctaaca ccgaggaggg acggacccgc gcacatcttc gagcgggccc ttggcccgca
     8881 tgaaggccgc cgtgcacacg aggtacggac cggccgacgt cgttcggatc gtggacttgg
     8941 agccgccgat cgccgcggcg agtggggtat tcgtcaaggt gcgtggctcc gtaggatgag
     9001 ctctcgtcac cgatcccggg aggtgtctga tgaccgaaca cgcgaaatcg gagccatccg
     9061 gcgatcgcgg ccggcccgcc gcaccaccgc ctcctcccgc ttggcgcaac tggctgctcg
     9121 tcgtgggtct cgtcgtctcc gtgctgatcc tgttctggcc tcggggcaca cgcagcgtgt
     9181 cggagctcag ctacaccgag ttcctctcgc gcgtgaaagc cggcgaggtc gcgacggccg
     9241 tcatcgaccc cggcggcgcc gtctccggga cgctcgagaa cggtggggac tacacgaccc
     9301 agatcccgat cgctctccag gaccccggcc tcgcggagga gctacgggcg aacggggtcg
     9361 agatcaccgg caaagccgag cccggggcca ccctcctgag cgtcatcctc agcttcctgc
     9421 cattcctgct gttgatcggg gtgtggctgt acatcggcag gcgcacgcag cgccagctcg
     9481 cgggcgggat cggcgggatc ctcggctcgc gcgcgaaggt ctacgacgcc gaggccccgt
     9541 cgacccgatt caacgacgtg gccggctacg agggcgccaa gcaggagatc agcgaggtcg
     9601 tcgacttcct gaagaacccc cagcgctacg cgaaggccgg cgcgatgggt ccgcgaggtg
     9661 tgctgatggt gggtccgccg gggaccggca agacgctcct cgcgcgggcc gttgccggcg
     9721 aggccggggt ccccttcctc gcgatcaccg gctcgggctt cgtggagctg ttcgtcggtg
     9781 tcggggcctc gcgcgttcgc gacctgttca ccgacgcgaa gaagcgggca ccggcgatcg
     9841 tcttcatcga cgaggtcgac gcgatcggcc agcggcgcgg ggcggg
//
LOCUS       DATBRK010000205         4639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2055418, whole genome shotgun sequence.
ACCESSION   DATBRK010000205 DATBRK010000000
VERSION     DATBRK010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055418"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VIH70_06185"
     CDS             complement(<1..464)
                     /locus_tag="VIH70_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1-2)-phosphatidylinositol
                     mannosyltransferase"
                     /protein_id="HEY8115984.1"
                     /translation="MDVPRTLLVTNDYPPRVGGIQRTLEALVRCFPPDRVAVLCPSAE
                     GGDAFDDVAPYTVFRQPERFLWPVRDVARRLLEAVGSFGAEVVLFGAVYPLALLGPSL
                     AETGTPYLAAAHGFEYWLSIAPGTHALVRRATSRAARVPVMCSAFIARVVRTA"
     gene            485..1321
                     /locus_tag="VIH70_06190"
     CDS             485..1321
                     /locus_tag="VIH70_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY8115985.1"
                     /translation="MDGAAFDEQLDALEQGRAFVDLQGWGLTEVRGADAEGWLNDLVT
                     ASVDGILEGRALRSFLLSPTGRIRADLHVLRARSGDGFLLLQDPDQPDAIADLLGPYV
                     LSSDVELAPADPRGLLVKPRSGLGWTTAWETPEDGSVPAGPEALEAWRIRNGLARFPV
                     DLDADSLPAEADLDVEPLIDRSKGCYLGQESVARVRNLGHPPRVVVGVRADLPLRPGA
                     PVRSQGAQVGSVTSVEPRGGSSAMVRIRWDARDTQLTADGGIALERRESPIGSPPILR
                     GP"
     gene            1431..1709
                     /locus_tag="VIH70_06195"
     CDS             1431..1709
                     /locus_tag="VIH70_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY8115986.1"
                     /translation="MTKDRIAGIDLKALPPDPSPPDIWQDQAACYGIDPDTFFPTSEE
                     EAGPALAFCTACDIRETCLSWALKNGERYGVWGGLTEQQRRRVVRFVA"
     gene            1777..2814
                     /locus_tag="VIH70_06200"
     CDS             1777..2814
                     /locus_tag="VIH70_06200"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEY8115987.1"
                     /translation="MTSDTIRASAPLRISFVGGGTDFPHYYERHGGAVLSATIDHAAH
                     VSVAPRDDRQVRIRSLDLGHLVGYHLEHGPVYDGVMDLPKAAIERMGVPTGIDVDIAS
                     DAPPGSGLGGSSALVTACVGALAMLTHRSMAPGEVAELAHLIEREDLGIAGGWQDQFA
                     AAFGGFNLIEFDQRGVAVRPLALSPEAIGELRAHLLLCYTGRVRTDLGLIATQIRMYE
                     DGREDTIVGMKQLQAMAFEMRRELEDGNLDRLGPMLREAFENKKLMNPHVSEGTRIER
                     LLTVAADAGASGGKICGAGGGGYLLLACRPDRQPAVRSALEALGGQFTEFAFRARGVE
                     AHDGGRVWRPS"
     gene            2811..3371
                     /locus_tag="VIH70_06205"
     CDS             2811..3371
                     /locus_tag="VIH70_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEY8115988.1"
                     /translation="MSRRFVLLDRDGTINEEIGYVLRPDELRLIPGAGEALRELRGLG
                     LGLVVLTNQSPIGRGMLTVAELNEIHARLTDLLAAEGVTLDGIEFCPHGPNDGCACRK
                     PGTLMVERAAVALGFDPAEAWLVGDHGSDLRLGRAVGARTILVRTGHGDAELERGAGE
                     DADHVVADLREAAAIIRDDLLTSVAP"
     gene            3368..3949
                     /locus_tag="VIH70_06210"
     CDS             3368..3949
                     /locus_tag="VIH70_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY8115989.1"
                     /translation="MNLDRVREYLLETSRTAARTADECGTQIIGATAVIVESLRAGGK
                     ILLCGNGGSAGDAQHLATEFVSTLTVERRRDAIAAIALTTDTSLLTAVANDFGFDGVF
                     ERQVEALGREGDVLLGISTSGNSENVVRALATAKAAGIRTIALTGESSGKVGAIADIA
                     INVPSTQTSHIQEAHIAVGQLIAFLVEDELFPR"
     gene            4053..>4639
                     /locus_tag="VIH70_06215"
     CDS             4053..>4639
                     /locus_tag="VIH70_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8115990.1"
                     /translation="MDTVIEIDGLRKEYRRVRGSRTVAVDGLDLAVPRGGVFGFLGPN
                     GAGKTTTIRCLLGLVRPTGGRCRLLGQEPAGLHNVIGRVGSIVETPAMYPRFSARRNL
                     ELLGRISGIGRTRVREVLEEVGLGERADDQVKTYSLGMKQRLGIAGALLKDPEVLILD
                     EPANGLDPAGIVEVRKLVRRLGHEGRTVFVSSHIL"
BASE COUNT          679 a         1614 c         1700 g          646 t
ORIGIN      
        1 gcggttcgca cgacccgggc gatgaacgcg ctgcacatca ccggcacccg cgccgcccgc
       61 gacgtggcgc gccggaccag cgcgtgggtc ccgggcgcga tcgagagcca gtattcgaac
      121 ccgtgcgccg ccgcgaggta gggggtcccg gtctccgcca gcgagggtcc gaggagggcg
      181 agcgggtaga ccgcgccgaa cagaaccacc tcggccccga acgacccaac ggcctcgagg
      241 aggcggcgcg ccacgtcgcg gacgggccac aggaagcgtt cgggctggcg gaacacggtg
      301 tacggcgcga catcatcgaa cgcgtcgccg ccctccgcgc tcggacacag gaccgcgacc
      361 cggtccggcg ggaagcaccg gacgagcgcc tccagcgtgc gctggatccc gcccacgcga
      421 ggcgggtagt cgttggtgac cagcagcgtc cgcggaacgt ccacggcggc ttatcctcgc
      481 agacgtggac ggagcagcgt tcgacgagca gctcgacgcg ctcgagcagg ggcgcgcgtt
      541 cgtggacctc caggggtggg gcctgaccga ggttcggggc gcggatgccg aggggtggct
      601 gaacgacctc gtcaccgcct cggtggacgg catcctcgag ggccgggcgc tgcgctcgtt
      661 cctgctctcc ccgaccggca ggatccgcgc cgatctccac gtcctgcgag cacgctcggg
      721 cgatgggttc ctcctgctgc aggaccccga tcagcccgac gcgatcgccg acctgcttgg
      781 gccgtatgtc ctgtcgtcgg atgtcgagct cgcgccggcc gacccccgcg ggctcctcgt
      841 gaagccccgg tcgggtctcg ggtggacgac ggcatgggag acgccggaag acgggtcggt
      901 ccccgccggg ccggaggccc tcgaggcctg gcggatccgc aacggtctcg cccgtttccc
      961 ggtcgacctc gacgcggatt ccctgccggc cgaggccgac ctcgacgtcg agccgttgat
     1021 cgatcggagc aagggctgct acctgggcca ggagtccgtt gcccgggtgc ggaacctcgg
     1081 ccacccgccg cgggtggtcg tcggggtcag ggcggatctc ccgctccggc ccggcgcgcc
     1141 ggtccgttcg cagggagccc aggtggggag cgtgaccagc gtggagccgc gagggggatc
     1201 ctcggccatg gttcgtatcc gttgggacgc aagggatacc cagctcacgg ccgacggcgg
     1261 catcgccctg gagcgccggg aatccccgat cgggagcccg ccgatactac gggggccctg
     1321 acttttcaag actgttctcg ttcgaacgcg aacggtacaa gggtgtcctg cgcgcacggg
     1381 ccccctcggg gtcgcctgcg caactcgacg aggcacgcag ggagttgact gtgaccaagg
     1441 accggatcgc agggatcgac ctgaaggccc tcccccccga cccctccccc ccggacatct
     1501 ggcaggacca ggcggcctgc tacgggatcg acccggacac gttctttccc acctccgagg
     1561 aggaggcggg acccgcgctg gcgttctgca cggcctgcga catccgggag acctgtctgt
     1621 cgtgggccct gaagaacggc gagcgctacg gcgtgtgggg cggcctcacc gagcagcagc
     1681 gccgtcgcgt cgtccggttc gtggcctgag tccaccggtc cagggggccg ccgcggctgg
     1741 tcgcgtagcc ggaacgcgtc ccatacttgc gggcccatga cgtccgacac gatccgcgcg
     1801 agcgcgcccc tccggatcag cttcgtcgga ggcggcacgg acttccccca ctactacgaa
     1861 cgccacggcg gcgccgtcct gtcggcgacg atcgaccacg cggcgcacgt ctcggtcgcg
     1921 ccacgggacg atcggcaggt gcggatccgt tcgctggatc tcggtcatct cgtcgggtac
     1981 cacctcgagc acggacccgt gtacgacggc gtgatggacc tccccaaggc cgcgatcgag
     2041 cggatggggg tgccgacggg gatcgatgtg gacatcgcat ccgacgcacc ccccggcagc
     2101 gggctcggag gctcctcggc gctggtcacg gcgtgcgtgg gcgcgctcgc gatgctgacg
     2161 cacaggtcga tggcgccggg agaggtcgcc gagctcgccc atctgatcga gcgcgaggac
     2221 ctcgggatcg cgggcgggtg gcaggatcag ttcgcagcgg cgttcggggg cttcaacctg
     2281 atcgaattcg accagcgcgg cgtcgcggtc cggcccctcg cgctctcgcc ggaggccatc
     2341 ggagagctcc gggcgcacct gttgctctgc tacacgggcc gcgtccggac cgacctcggg
     2401 ctgatcgcca cccagatccg catgtacgag gacgggcgcg aggacacgat cgtggggatg
     2461 aagcagctgc aggcgatggc gttcgagatg cgccgggagc tcgaggacgg gaacctcgac
     2521 cggctggggc cgatgctccg cgaggccttc gagaacaaga agctgatgaa cccgcacgtc
     2581 tccgagggca cgcggatcga acgcctgctc acggtggccg cggacgcagg cgcctcgggc
     2641 ggcaagatct gcggcgccgg gggaggcggc tacctcctgc tcgcgtgccg gccggaccgc
     2701 cagcccgcgg tccgaagcgc gctcgaggcg ctcggcggcc agttcacgga gttcgcgttc
     2761 cgcgcccgcg gggtcgaggc gcacgacggc ggtcgcgtgt ggagaccctc gtgagccgcc
     2821 gcttcgtcct gctcgatcgc gacggcacga tcaacgagga gatcgggtac gtgctccggc
     2881 ccgacgagct ccgactgatc cccggtgcgg gagaggccct tcgcgagctc cggggactcg
     2941 ggctcggcct cgtcgtcctg acgaaccagt ccccgatcgg ccgcgggatg ctcaccgtcg
     3001 ccgagctcaa cgagatccac gcgcgcctga cggacctgct cgccgccgag ggcgtgaccc
     3061 tggacggcat cgagttctgt ccgcacgggc cgaacgacgg ctgcgcctgc cgcaagcccg
     3121 ggacgctgat ggtcgagcgc gccgccgtcg ccctggggtt cgaccccgcc gaggcctggc
     3181 tcgtgggcga tcacggatcc gacctgcgtc ttgggcgagc ggtgggcgcc cgaacgatcc
     3241 tcgtgcgcac cggccacggg gacgccgagc tcgagcgggg ggccggcgag gacgccgacc
     3301 acgtcgtcgc cgacctgcgt gaggcggcgg ccatcatccg ggacgacctg ttgacgagcg
     3361 tggcgccgtg aacctcgatc gggtgcgcga gtacctcctc gagacctcgc gcacggccgc
     3421 ccggaccgcc gacgagtgtg ggacgcagat catcggcgcg acggccgtga tcgtggagtc
     3481 gctccgggcc ggagggaaga tcctcctgtg cggcaacggc ggcagcgccg gcgacgccca
     3541 gcatctggcg acggagttcg tcagcacgct cacggtcgag cggcggcgcg atgccatcgc
     3601 ggcgatcgcc ctcacgacgg atacctcgct cctgacggcg gtggcgaacg acttcggctt
     3661 cgacggagtg ttcgaacggc aggtggaggc cctcggtcgc gagggcgacg tgctcctcgg
     3721 gatctcgacg agcgggaact cggagaacgt cgtgcgtgcg ctggcgacgg cgaaggcggc
     3781 cggcatccgg acgatcgctc tgaccggcga gtcgagcggc aaggtcgggg cgatcgccga
     3841 catcgcgatc aacgtgccga gcacgcagac ctcccacatc caggaggcgc acatcgcggt
     3901 gggtcagctg atcgcgttcc tggtcgagga cgagctcttc ccccgctgac cgccccgacc
     3961 gaacctctcc ggaccgcgtg ctaaccaccg gtgatctgtg gtgggtaccc cccgtgccct
     4021 cgaggcgtgc gccgacgtac cgtgtccgtg ggatggacac cgtcatcgag atcgatgggc
     4081 tccgcaagga gtaccggcgg gtccgcggca gccggaccgt ggcggtcgac ggcctggatc
     4141 tggccgtccc gcgaggcggc gtgttcggct tcctcgggcc gaacggtgcc ggaaagacca
     4201 cgacgatcag atgcctcctg ggcctcgtcc gtccgaccgg tgggcggtgc cgcctgctcg
     4261 gccaggagcc cgcggggctc cataacgtca tcggccgggt cggctcgatc gtcgagactc
     4321 cggcgatgta cccgcgcttc tcggcgaggc gcaacctcga gctgctcgga cggatctcgg
     4381 gcatcgggcg cacgcgcgtc cgcgaggtgc tcgaggaggt cgggctcggc gaacgcgcgg
     4441 acgatcaggt caagacgtac tcgctcggca tgaagcagcg gctcgggatc gcgggggcac
     4501 tcctcaagga tcccgaggtc ttgatcctcg acgagccggc gaacggactc gatccggccg
     4561 ggatcgtcga ggtccgcaag ctggtgcggc gactcgggca cgagggaaga accgtcttcg
     4621 tctcgagcca tatcctgaa
//
LOCUS       DATBRK010000206         6906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2197433, whole genome shotgun sequence.
ACCESSION   DATBRK010000206 DATBRK010000000
VERSION     DATBRK010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197433"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..942)
                     /gene="lepB"
                     /locus_tag="VIH70_06220"
     CDS             complement(142..942)
                     /gene="lepB"
                     /locus_tag="VIH70_06220"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8115991.1"
                     /translation="MAEIEHADDDARDQPDAVALATPEPAASLHAAEETDVEPSADLR
                     DHDGHPGAPTRHEPERDDEDGRDSAWRRFVGSTPFLILVALAVAILVKTFVIQAFYIP
                     SESMVPTLEVGDRVFVSKFMFDGGDIARGDVIVFENPNVAELPDRSGISSVLHWLGEG
                     VGLAQPENEDFIKRVIALPGETIEIRRDVVYINGKPLDEPYLTQAAKDSTGDYPPHTV
                     PDDALFVMGDNRGNSADSRYGLGFVPFDKVIGKAFVIIWPPSHMGGLG"
     gene            complement(971..2044)
                     /locus_tag="VIH70_06225"
     CDS             complement(971..2044)
                     /locus_tag="VIH70_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8115992.1"
                     /translation="MTLSVNVTIRRGTFAVRASFDARRGSTTAVLGPNGSGKSTLVSS
                     MAGLVPPEEGSIVLDGTVLDEPAAGVYLPPERRPIGVVFQDLLLFPHLSALENVEFPL
                     LARGVARREARDRAEHLLRRLEVVERARAEPRDLSGGEAQRVALARALVAEPALLLLD
                     EPLSALDVGAGARLREILRDELERFPGIRILITHDPVEAFTMADRLVIVEDGRVTQIG
                     TPEEIRSAPRSRYAADLVGVNAVRGVLEPIGEGTGQLISSEGTLVVPWPEWYVEGEVV
                     GIVRPIDVTLWPDRPQGSARNVLSGRVTSVAVERDRARVRLATAPPLVAEVTLGSVER
                     LRLRSGVEVWASFKAVEVELLPP"
     gene            complement(2041..2868)
                     /locus_tag="VIH70_06230"
     CDS             complement(2041..2868)
                     /locus_tag="VIH70_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8115993.1"
                     /translation="MATDSERSPGTGSSPLRRGRIPAGFVALAALGVALVALPLLGLL
                     IRTPWSRVGEILTSETALTALRLSVVVATTAAAISFVLGFPLAWALARGSFRGRRFVR
                     ALIVLPLVMPPVVAGVSLLAAFGRRSGLIGSWLYEWFGIQLTFTTAAAVLAATFVSFP
                     LAVLALEAGLRGLDERLEDAASTLGAPRRYVLRRVTLPLMGPQIAAALVLSWARALGE
                     FGATITFAGNLQGRTQTLPLAVFERLQTDPEAAFAISMLLIAVAFAVILALRGRFLR"
     gene            complement(2816..3616)
                     /gene="modA"
                     /locus_tag="VIH70_06235"
     CDS             complement(2816..3616)
                     /gene="modA"
                     /locus_tag="VIH70_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8115994.1"
                     /translation="MTRPGFTVAVVLVSLVATACLGDGPNTESGDTASAQEGGDLTVS
                     AASSLTDAFTEIGKAFETTNPGTTVTFNFGPSDGLAAQIDQGAPVDVFASASPTWMDS
                     VQDDGPGVTDRADFARNRLAIIVPVDNPAGIENLDDLTEDRVQLVLAAEGVPAGDYAR
                     EMLDEAGISEAALANVVSNEEDVRAVITKLIVGDADAGIGYVTDVTPTVSDRVALIPI
                     PDRVNVVSTYPIAVLNGTQEADLAKDFVDFVLGDGQRTLARYGFLPPA"
     gene            complement(3613..4005)
                     /locus_tag="VIH70_06240"
     CDS             complement(3613..4005)
                     /locus_tag="VIH70_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY8115995.1"
                     /translation="MTEESYRIGAAASMLGVRVETLRRWEREGKLSTTRTAGGQRTVA
                     AAEVARLLAEGRERPRITSASVRNRFPGTVTEIKRDRLAATVEILSGPHRILAFVTRE
                     AVDEMGLQPGMRAVATVKATNVMLEVDL"
     gene            complement(4093..5070)
                     /locus_tag="VIH70_06245"
     CDS             complement(4093..5070)
                     /locus_tag="VIH70_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEY8115996.1"
                     /translation="MKDIGLVGVPFSGKSTLFTALTRTGGHGGRANHAVVPVPDPRVD
                     VLTQMERSRKTVHAQVGFVDVPGGTASAQGIAKLREADALAIVVRCFGPDAEPVSEVA
                     EVRADLLLADQAVIEPALEKAEKRARGKRTPEVEALAAANAALQGEVPLSDAGLSEEH
                     RRELKPIAPLTIKPSIVVANLEEGTDVPEGLPEGTVGVYASIEAETAEMDPADARALL
                     DEFGVHEPGLETVIRAGYSAIDLITFLTTGDDETRAWEVRSGAKAPEAAGVIHTDLER
                     GFIRAEVIAYDELVAAGSMDAAKQQGKVRVEGKDYEVREGDILHVRFAV"
     gene            complement(5166..5582)
                     /gene="sodN"
                     /locus_tag="VIH70_06250"
     CDS             complement(5166..5582)
                     /gene="sodN"
                     /locus_tag="VIH70_06250"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEY8115997.1"
                     /translation="MLKASLRPRRVVHAHCDLPCGIYDPSQARVEAESVKAIQTRYQD
                     AEKFKTSGESVEDYRIRALAIKEARADLVKEHLWVLWTDYFKPEHLEKYPQLHELFWN
                     ATKEAGLAKKAQDPAQGQKLLDAIDEIGKIFWETKA"
     gene            5613..6056
                     /locus_tag="VIH70_06255"
     CDS             5613..6056
                     /locus_tag="VIH70_06255"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEY8115998.1"
                     /translation="MTRTYHRGAERRKVAFAAVVLAGIAAAYGLRRWRPFRIEVEGAS
                     MRPTLEPEDWAVAVRARRLHRGDVVVVEHPDRVGFELVKRVTHLPGDVAPDGLGLVDR
                     VWVEGDDPEGSSDSRTFGPVPLGLVRGRVWFVWWPPGRIRAPRPR"
     gene            6270..6500
                     /locus_tag="VIH70_06260"
     CDS             6270..6500
                     /locus_tag="VIH70_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY8115999.1"
                     /translation="MDVITATVDARGQSCPGPLVALHKALGNAGPGDLLELLATDPGS
                     KSDVPSWSKLSGHELLEASETSGVYRYVIRKV"
     gene            6506..>6906
                     /locus_tag="VIH70_06265"
     CDS             6506..>6906
                     /locus_tag="VIH70_06265"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEY8116000.1"
                     /translation="MVQTAGEATDETGTEAMRELIDQALAEREATDGFNRKNKITIVA
                     WDGHLDRVWPTLILSTTAAASGMEVSVFFTFWGLFALVKPERRRKTGRDWMTKALGQM
                     NPGSTAKAKLSRYNFAGMGPWMLGKVAEDYKT"
BASE COUNT         1011 a         2442 c         2427 g         1026 t
ORIGIN      
        1 tgatcgtgtg gctggcggcg ttcggcatcg cggcgctggt gtggtcgttc gcgcggctgt
       61 tcctgccgcc ggagcggccc gtgggtcccg gcgctccgcc ggcgcccgcc tgagcgttcg
      121 cgccgcggcg gttcggtgcg gttagccgag cccgcccatg tgcgacgggg gccagatgat
      181 cacgaacgcc ttgccgatca ccttgtcgaa cggaacgaat ccgagaccgt acctgctgtc
      241 ggccgagttc ccgcggttgt cgcccatcac gaacagagca tcgtccggaa cggtgtgcgg
      301 cgggtagtcg ccggtgctgt ccttcgccgc ctgggtcagg tacggctcgt cgagcggctt
      361 gccgttgatg tagacgacgt cgcggcggat ctcgatcgtc tcgcccggca acgcgatcac
      421 gcgcttgatg aagtcctcgt tctccggctg cgcgagtccg accccctccc cgagccagtg
      481 caggacgcta ctgatgccgc tgcgatcggg cagctccgcg acgttcgggt tctcgaacac
      541 gatcacgtcg ccgcgcgcga tgtccccgcc gtcgaacatg aacttgctca cgaacacccg
      601 atcgccgacc tcgagcgtgg ggaccatgga ctcggacggg atgtagaacg cctggatcac
      661 gaaggtcttc accaggatcg cgacggcgag cgcgaccagg atcaggaacg gcgtcgagcc
      721 gacgaaccgg cgccacgccg agtcgcggcc gtcctcgtcg tcgcgctcgg gctcgtgccg
      781 ggtcggggcg ccggggtggc cgtcgtggtc gcgcaggtcg gcgctcggct ccacgtcggt
      841 ctcctctgcc gcatgcaacg acgctgccgg ctcgggggta gcgagcgcga cggcgtcggg
      901 ctggtcgcgg gcgtcgtcgt ccgcgtgctc gatctcggcc atggccaccc aggatacgtg
      961 ttcggatccc ctacggaggg agcagctcga cctcgacggc cttgaaggac gcccagacct
     1021 cgacgccgga gcgcaggcgc agccgttcga ccgagcccag ggtgacctcc gcgacgagcg
     1081 gcggtgcagt cgcgagccga acgcgcgcgc ggtcgcgttc gaccgcgacc gaggtgacgc
     1141 gaccggacag gacgttgcgc gcggatcctt gggggcgatc cggccacagc gtcacgtcga
     1201 tcggtcgtac gatgcccacg acctcgccct ccacgtacca ctcgggccac ggtacgacca
     1261 gcgttccctc gctcgagatc agctggccgg tcccctcgcc gatcggctcg aggacgcccc
     1321 ggaccgcgtt caccccgacg aggtccgccg cgtagcgcga ccgcggcgcc gagcggatct
     1381 cctcgggcgt cccgatctgc gtcacgcgtc cgtcctcgac gatcacgagc cggtcggcca
     1441 tcgtgaacgc ctccaccggg tcgtgggtga tgaggatccg gatccccggg aagcgttcga
     1501 gctcgtcccg gaggatctcg cgcagccgcg cgcccgcgcc cacgtcgagc gccgacaggg
     1561 gctcgtcgag cagcagcagc gcgggttcgg cgacgagcgc gcgggcgagc gccacgcgct
     1621 gggcctcgcc gccggagagg tcgcgcggct cggccctggc gcgctcgacc acctcgagcc
     1681 ggcgaaggag gtgctcggcg cggtcgcgcg cctctctccg ggcgacgccg cgtgccagga
     1741 gcgggaactc gacgttctcg agcgccgaca ggtgggggaa cagcaggagg tcctggaaga
     1801 ccacgccgat cggtcgccgc tcgggtggga ggtagacgcc tgcggccggc tcgtccagca
     1861 ccgtcccgtc caacacgatc gagccctcct cgggcgggac gagcccggcc atcgaggaga
     1921 cgagcgtcga cttgcccgaa ccgttcggtc cgaggaccgc cgtggtggat ccccgtctgg
     1981 cgtcgaacga cgcccggacc gcgaacgtcc cccgtcggat ggtcacgttc accgacagcg
     2041 tcatcgcagg aaccgccccc gcagcgcgag gatcaccgcg aaggccacgg cgatcagcag
     2101 catcgagatc gcgaacgccg cctcggggtc ggtctggagc cgttcgaaca cggcgagggg
     2161 gagcgtctgc gtccgtccct gcaggttccc ggcgaacgtg atcgtcgcgc cgaactcgcc
     2221 gagcgcccgt gcccacgaca ggacgagcgc cgcggcgatc tgcggcccca tgagcggaag
     2281 cgtgacccgt cgaaggacgt accggcgggg cgcgccgagg gtcgacgccg cgtcctcgag
     2341 ccgctcgtcg aggccgcgca ggcccgcctc gagtgccaaa acggcaagcg ggaacgagac
     2401 gaacgtcgcc gcgagcacgg ccgcggcggt cgtgaacgtg agctggatgc cgaaccactc
     2461 gtacaaccac gatccgatca gcccgctccg ccgaccgaac gcggccagca ggctcacacc
     2521 ggcgacgaca gggggcatga ccagcgggag cacgatcagc gcgcgcacga acctcctccc
     2581 gcggaacgac ccgcgcgcga gcgcccacgc gagcgggaac ccgaggacga acgagatcgc
     2641 cgctgcggtc gtggcgacga ccaccgagag ccgaagcgcg gtgagcgccg tctcgctcgt
     2701 caggatctcg cccacccgcg accagggcgt gcggatcagc aggccgagca acggcagtgc
     2761 gacgagcgcg acgccgagcg ccgcgagggc gacgaagccg gccgggatcc gccccctacg
     2821 caggggggag gaacccgtac cgggcgagcg ttcgctgtcc gtcgccaagg acgaagtcga
     2881 cgaagtcctt cgcgaggtcg gcctcctggg ttccgttcag gaccgcgatc ggataggtcg
     2941 agacgacgtt cacccggtcg gggatcggga tcagcgcgac ccggtccgag acggtgggcg
     3001 tgacgtcggt gacgtagccg atgccggcat ccgcgtcgcc gacgatcagc ttggtgatga
     3061 cggcccgcac gtcctcctcg ttggagacga cgttcgcgag tgccgcctcg gagatccccg
     3121 cttcatcgag catctccctg gcgtagtccc ccgccggaac gccttcggcg gcgaggacca
     3181 gctggacccg atcctcggtg aggtcgtcga ggttctcgat cccggccggg ttgtcgaccg
     3241 gcacgatgat cgcgaggcgg ttccgagcga agtccgcgcg gtccgtcacg cccggtccgt
     3301 cgtcctggac cgagtccatc caggtcgggc tcgcggaggc gaagacgtcg acgggggcgc
     3361 cctgatcgat ctgagccgcg agcccgtcgg acggtccgaa gttgaaggtc acggtcgtgc
     3421 cggggttcgt cgtctcgaac gccttcccga tctcggtgaa cgcgtccgtg agcgacgagg
     3481 ccgcggagac cgtcaggtcg cccccctcct gcgcggacgc ggtgtcgcca ctctcggtgt
     3541 tcggtccgtc gccgagacac gccgtggcga cgagcgagac gagcacgacc gcgaccgtga
     3601 acccggggcg cgtcacaggt ccacctcgag catcacgttg gtggccttga cggttgcaac
     3661 ggccctcatc ccgggttgga ggcccatctc gtcgacggcc tccctcgtga cgaacgcgag
     3721 gatcctgtgc ggacccgaga ggatctcgac cgtcgccgcg agcctgtcgc gcttgatctc
     3781 ggtcaccgtt ccggggaacc ggttccgaac gctcgcggag gtgatcctcg gacgctcgcg
     3841 accctcggcg agcaggcggg ccacctcggc ggctgcgacc gtgcgctggc cacccgccgt
     3901 ccgggtggtg ctgagcttcc cctcgcgctc ccagcgacgc agcgtctcga cgcggacgcc
     3961 gagcatcgag gccgccgcac cgatccggta ggactcctct gtcacgccgg tgagtctagg
     4021 tagtttcccg tcggcatcgc cagagatagc tggcgatgtc ttgccagaag cacgcgagga
     4081 gccgggccga cctcacacgg cgaagcggac gtggaggatg tcgccctcgc ggacctcgta
     4141 gtccttgccc tcgacccgca ccttgccctg ctgcttggcg gcgtccatcg agcctgccgc
     4201 gaccagctcg tcgtacgcga tcacctcggc gcggatgaac ccgcgttcga gatcagtgtg
     4261 gatcacgccc gcggcctcgg gcgccttcgc cccgctgcgg acctcccacg cgcgcgtctc
     4321 gtcgtccccg gtcgttagga acgtgatcag gtcgatcgcc gagtagcccg ctcggatcac
     4381 ggtctcgagc ccgggttcgt gcaccccgaa ctcgtccagg agcgcccgcg catcggcggg
     4441 atccatctcc gccgtctcgg cctcgatcga ggcatacacg ccgaccgtcc cctcgggcag
     4501 accctccggg acgtcggtcc cttcctcgag gttcgcgacg acgatcgacg gcttgatcgt
     4561 gagcggagcg atcggcttga gctcgcgccg gtgctcctcg gaaagcccgg cgtccgagag
     4621 cgggacctcc ccctggaggg ccgcgttcgc ggcggcaagc gcctccacct cgggcgtccg
     4681 cttcccgcgg gcccgcttct ccgccttctc cagcgccggc tcgatcacgg cctgatccgc
     4741 gagaagaagg tcggcgcgga cctccgcgac ctcggacacg ggctccgcat ccggcccgaa
     4801 acatcgcacg acgatcgcga gggcgtccgc ctcgcgcagc ttcgcgatgc cctgcgccga
     4861 ggcggtcccg cccgggacgt cgacgaaccc gacctgggcg tgcaccgtct tgcgcgagcg
     4921 ctccatctgg gtcagtacgt cgacgcgggg gtcgggaacg ggcaccacgg catggttggc
     4981 ccggcccccg tgaccgcctg tccgcgtgag cgcggtgaac agcgtcgact tcccgctgaa
     5041 cgggacgccg accagtccga tgtccttcac gcgggcagtc taggaggcga cccgagaccg
     5101 atccggtccg ggacgcgacg aggggcggcg ccccgagccc gcgttcgtgt tcggaaccgc
     5161 gctgcctacg ccttggtctc ccagaagatc ttgccgatct cgtcgatcgc gtccagcagc
     5221 ttctggccct gggcggggtc ctgcgccttc ttcgccagac ccgcctcctt ggtcgcgttc
     5281 cagaacagct cgtggagctg cggatacttc tcgagatgct cgggcttgaa gtagtcagtc
     5341 cacagcaccc acaggtgctc cttgacgagg tcggcgcgcg cctccttgat cgcgagcgcg
     5401 cggatccggt agtcctccac gctctccccg gaggtcttga acttctccgc gtcctggtag
     5461 cgggtctgga tcgccttcac cgactcggcc tccacgcgag cctgcgacgg gtcgtagatc
     5521 ccgcagggca ggtcgcagtg cgcatggacg acgcggcgtg gtctgagcga cgctttcagc
     5581 atgggttccc tccttcggac gatcggggat ccgtgacccg aacctaccac cggggtgcgg
     5641 aacggcggaa ggtggcgttc gcggcggtcg tcctcgccgg catcgctgcg gcgtacggct
     5701 tgcggcgctg gcgtcccttc cggatcgagg tcgagggcgc gtcgatgcgt cccacgctcg
     5761 aacccgagga ctgggccgtc gcggtccggg cccggcggtt gcatcgcggc gatgtcgtgg
     5821 tggtagagca tccggatcgc gtgggcttcg agctcgtcaa gcgcgtcacc cacctgccgg
     5881 gggacgtggc gcccgacggg ctcgggctgg tcgatcgggt gtgggtggag ggggacgatc
     5941 ccgagggctc gagcgacagc cggacgttcg ggcccgtgcc gctgggactc gtgcgcggca
     6001 gggtctggtt cgtgtggtgg ccgcccggcc ggatccgggc gccgaggccc aggtgagccg
     6061 gttgcgccat ttcccatctg cccgatagca tttgcagttg caatgatccg gacacgctgc
     6121 cgaggtctcg tgggggccat gatccccgac cgatgctgct gtccacgggt gccccgcggc
     6181 cgccgctagg cgtcgcagca ggcaccgagc ccgcccaccc cgtaccgaag agccgccacc
     6241 acgttccgct cgagaggagg tctccgccga tggacgtcat caccgccacc gtggacgctc
     6301 ggggccagtc ctgccccggg ccgctcgtcg cgttgcacaa ggcgctggga aacgcgggcc
     6361 cgggcgacct gctcgagctg ctggcgaccg atcccggctc gaagtccgac gttccgtcgt
     6421 ggtcgaagct gtcgggacac gagctcctcg aagcgagcga gacctcaggc gtctaccgct
     6481 acgtgatccg gaaggtgtga tcgagatggt gcagaccgcg ggcgaggcga ccgacgagac
     6541 cgggaccgag gccatgcgtg agctgatcga ccaggcgctg gccgaacgcg aggcaaccga
     6601 tgggttcaac cggaagaaca agatcacgat cgtcgcctgg gacggccacc tcgaccgggt
     6661 gtggccgacg ctgatcctgt cgacgaccgc ggccgcctcc gggatggagg tgagcgtgtt
     6721 cttcacgttc tgggggctgt tcgcgctcgt gaagcccgag cggcgccgca agaccgggag
     6781 ggactggatg accaaggcgc tcggccagat gaaccccggt tcgacggcca aggccaagct
     6841 ctcccggtac aacttcgccg gcatgggccc ctggatgctc ggcaaggtgg ccgaggacta
     6901 caagac
//
LOCUS       DATBRK010000207         4337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_318104, whole genome shotgun sequence.
ACCESSION   DATBRK010000207 DATBRK010000000
VERSION     DATBRK010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318104"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(771..2060)
                     /locus_tag="VIH70_06270"
                     /pseudo
     CDS             complement(771..2060)
                     /locus_tag="VIH70_06270"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(2065..3492)
                     /locus_tag="VIH70_06275"
     CDS             complement(2065..3492)
                     /locus_tag="VIH70_06275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8116001.1"
                     /translation="MQTVDIAPAPADRFDAFVAAEPMGRFLTVLEAGADRFADRTLWH
                     MNSTSQGGGVAEMLQSLLGYLVGAGIRTRWMVIDGEDSFFDVTKRIHHRLHGKRGDAG
                     PLGASERETYERNLADDARELVEMVRDGDVVVLHDPQTIGLAPALRESGAHVVWICHV
                     GVDEPNKLAREAWGFLRPYVDSTDAQIFSRRSYVWEGLRPEHVEVLPPCIDAFAPKNQ
                     ALDGSSVRAILRVAGLIDDNGAGGIPSFVRRDGTKGIVTRVAHVEGGPLPARSRIVLQ
                     VSRWDPLKDPVGLLEAFVAHIPSDPSIRLVLAGPETEATVDDPESEETLAKVRAVRDR
                     LPTPSRSRVHLASLPMGDVDENASIVNALQRQAAVVVQKSLAEGFGLTVAEAMWKERA
                     VVASRVGGIQDQIVDGVSGALVDPTDPSAVGDAIANLLRDPERAAEMGRRGHARVCEE
                     FLPPRYLTRLLELVERIAGRQRIED"
     gene            complement(3846..>4337)
                     /locus_tag="VIH70_06280"
     CDS             complement(3846..>4337)
                     /locus_tag="VIH70_06280"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY8116002.1"
                     /translation="PTELDVPLDFDSMRQVGSGLGSAGFAAFDDSACMVRAAHLYSRF
                     LYVESCGQCPACKFGTGETTRLLAEVESERASDQSVESILIRARGSTDGQKCALPTGE
                     SLLIQSLVQVFANEFRGHVGRACPLPRELVFHKIVDWDAANDRFVYDESYQRKRPDWT
                     YAP"
BASE COUNT          775 a         1419 c         1464 g          679 t
ORIGIN      
        1 gtcgggccag tcgccgtcgg gccggtcggg ccggtcgatc cggtcgacgc ggtcggtccc
       61 gtcggacccg tttccgtcgt gtcgttcgac gacgcgggga cgacgaacgg attctcgggc
      121 gtgccgggat tcgagccacc gaggtagagc acggcggcga gaatcgtcgc gccagccaat
      181 gccccggccg atccgagcgc gaacgccaat cgcgccccac ggcggtctcg gagacgttct
      241 gtgcgacgcc ggcgctcgag ctccggtgag agcttgccgc gcacgttcag ccacgacatg
      301 tacagcgacg tcgcgtagac gcttccctgc caggcgaaca aaccgatcag gaacggcgtc
      361 gccttccccg tgagtgcagc cgcgaccccg gccgcccaga gcagcaacgc gatgaacgtc
      421 tcgggtttgg ccatccggag cgcgtcgatc gctcgcgggt cctcggacgt cttcggcgtc
      481 cgcatgaaca ccatgtccga gcgaacgagg ccctggatgc acgtgagcgc gaccgtccac
      541 gaaagcgaca accagttcgc gaaggctcga accgccctca ccgtgccgat cccggtcgtg
      601 gcgcgcagag cccacatcgc gcgcagaagg ccggagatga tcagcgtcgc cggaagcagg
      661 acgaccgcac cgaggagtgg ccggacgccc acgcgaccct gggtgacgag caatgcggcc
      721 gtcgtaagca gcaccaccga gaaccccagg tacagcaggt cgttgaacca ctgcaggctg
      781 ctgaagaggt agtccatccg ttgaccgaag ctgagctggt tccctggatc gcggttccac
      841 gggaggaggt cccgcaggtg ccgcttgagg atctggatgc caccgaagca ccagcggaac
      901 cgctggctct tcagcgacgc gaacgtgagt ggcatgatcc ccttgccgaa gctgcggtgg
      961 atgtagagac ccgagtagcc cgccttcagc atccgcaggg acgccagcga gtcctcggtg
     1021 atgcaccact cgtcccagcc gtggagccgc tcgagcacgg aacgacggat gagacccatc
     1081 gtgcccgcga agatgatcga gtcacgttgg ttccgggagg gcatcgacgt cgcgaagaag
     1141 taccggtaag cgtcgtagca cgccgtgagg taccggtcgc tctcccagtc gcgatagtcc
     1201 tgcggcgtct ggacgaacgc gatgtgcgga tcggcgaagt agccgacgac cgacagcagg
     1261 tagctcggat cgagcaggta gtccgcgtcg acgacgccga cgatctcggc gtcgcgtgcc
     1321 gtgtgctcgg ccagcgcgag gttgagcgcg ccggccttga acccctcgag accctcgacg
     1381 tgcacgaact tcacccgggg ccggtcacgg cagtagtcct cgaccggacg ccagaccgcc
     1441 ggatcctcgg tgttgttgtc gatcaccacg atctcgagat cgggatagtc cagagcctcc
     1501 acagacgcga tcgtttcgat cagcatgtcg ggtggctcgt tgtacgccgc gatctgcaat
     1561 gacaccttcg gcaggtaggc cgggtttggt tcggcgaccg gtcggtccca tcggatgcgg
     1621 gccgccgaat cgcatgtgtc gaacgcgtac cacgacgtga gcgtgagtgc ggccagctcg
     1681 agcaggaaca ggagtcccga cgcgagggcg ccgacgagag acagtccgcc aaagagtgtg
     1741 taccaggccg cgaacgacag gtaggtaacg gccgacgcga gcagcgtgcc gtagaagacc
     1801 tggccgatcg ggttccattg gggtcgaagg aggatgatcg cgatgccgaa gatgaccaga
     1861 ccggcttggg cggcgaggat gtgctcgcgg ggcacatcga agagcactat cgcgagctgt
     1921 ccggcgacga tcgcgacggc tatgtcgaga agcagcccga gcaccctcga ggtccggtgc
     1981 cctcggtggc cggccaggag caggaggatg gtcgtgatcg ccagagcgac aacgcacgtg
     2041 aggaagagac cgaggaacat ctggtcagtc ctcgattcgc tgacgtccgg cgatgcgctc
     2101 cacgagctcg agcaagcgtg tgaggtagcg cggcggcagg aattcctcgc agacgcgagc
     2161 gtgccctcgg cggcccatct cggcggcgcg ctcgggatcg cgcaggaggt tcgcgatcgc
     2221 gtcgccaacg gccgacgggt ccgtggggtc gacgagcgca ccgctcacgc cgtcgacgat
     2281 ctggtcctgg atgccgccga cgcggctcgc gaccaccgcg cgttccttcc acatcgcttc
     2341 ggcgaccgtg aggccgaacc cttcggcgag gctcttctgc acgacgacgg ctgcctgtcg
     2401 ctggagcgcg ttcacgatcg aggcgttctc gtcgacgtca cccatcggca ggctcgcgag
     2461 atgaacgcgg ctccgggacg gggtcggaag acggtcgcgg acggctcgga ccttggcgag
     2521 agtctcttcg gactcgggat catcgaccgt cgcttccgtc tcgggtccgg ccagaaccag
     2581 gcggatggat ggatcggacg gtatgtgagc gacgaaggcc tccagcaggc cgaccggatc
     2641 cttcagcgga tcccagcggg agacctgcag aacgatgcgg ctccgcgccg gaagcgggcc
     2701 gccctcgacg tgggcgaccc gtgtaacgat gcccttggtt ccgtcccgcc gtacgaacga
     2761 ggggatgccg cccgctccgt tatcgtcgat caacccggcg acgcgcagta tcgctcgaac
     2821 cgaggagccg tcgagcgcct gattcttcgg agcgaaggcg tcgatgcacg gcggcagcac
     2881 ctccacgtgc tccggtcgca gcccctccca cacgtagctc cggcggctga agatctgggc
     2941 gtccgtcgag tccacgtagg ggcgcaggaa cccccacgcc tctcgcgcga gtttgttcgg
     3001 ctcgtcgacg cccacgtgac agatccagac gacgtgggca cccgactcgc gaagtgcggg
     3061 tgcgaggccg atcgtctgcg ggtcgtggag cacgaccacg tcgccgtcgc gcaccatctc
     3121 gacgagttca cgcgcgtcgt ccgccaggtt gcgttcgtag gtctctcgct cggacgcacc
     3181 aagtggtccg gcgtcgccac gtttgccgtg aagccgatgg tggatgcgct tcgtgacgtc
     3241 gaagaaggaa tcctcgccgt cgatcaccat ccagcgagtc cggatcccgg cgccgacgag
     3301 gtacccgagc aatgactgaa gcatctcggc gaccccaccg ccctgcgagg tcgagttcat
     3361 gtgccacagg gtccgatcgg cgaaccgatc ggcgccagcc tcgagcacgg tgaggaagcg
     3421 gcccatgggc tccgcggcca cgaatgcgtc gaagcggtcc gccggagcgg gtgcgatatc
     3481 gacggtctgc atgacaggtg ggcttctctt tcccggacct ggggtccgcc aacccttctc
     3541 ttgcaacgag gcctgctcgc gctcatcaca aggggtgaga gctcgtcagg tctcttcgtg
     3601 gttctcagcg gcggcgactc ggcgggcgtc aggccgctca agcggactcg cccagcgacc
     3661 gatcgagcgc gccagcggtt ctgggaagag acggtcagcc aaaactacta cgacggccaa
     3721 aggagatatc accttccatc gaaggcgacc gtctgactgg gtggcccacc ctgaaaggcg
     3781 acgcccgcga gcgggtataa ctcgaagacc gcctaggtcc aagggtggat cgtcctgcgg
     3841 aaaggtcagg gcgcgtacgt ccagtcgggc cgtttcctct ggtagctctc gtcgtagacg
     3901 aatctgtcgt tcgctgcgtc ccagtcgacg atcttgtgga agaccagctc acgtgggagg
     3961 ggacatgcac gaccgacgtg gccgcggaac tcgttcgcga acacctgcac caggctctgg
     4021 atcaggaggc tctccccagt cggcagtgcg cacttctgcc cgtcggtcga tcccctcgcc
     4081 cgtatcaaga tcgattccac cgactggtcc gacgcccgct ccgactcgac ttctgccagg
     4141 agcctggtcg tctctccggt gccgaacttg caggcgggac attgtccgca ggactccacg
     4201 tacaggaacc gtgaatacag gtgcgcggcc cgcaccatgc aggccgagtc gtcgaatgcg
     4261 gcgaagcccg ccgatccgag gccgctgccg acctggcgca tcgaatcgaa atccagcggg
     4321 acgtccaact cggtggg
//
LOCUS       DATBRK010000208         4544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1043108, whole genome shotgun sequence.
ACCESSION   DATBRK010000208 DATBRK010000000
VERSION     DATBRK010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043108"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VIH70_06285"
     CDS             complement(<1..264)
                     /locus_tag="VIH70_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116003.1"
                     /translation="MTEEIPNFYVGNKSKKIVCHPMINKNKEAGKPFKIKAYAQDFTW
                     HKRQYTVETSRMITDNKGIGHIYFDANDSAILSMDIDHEKKCIK"
     gene            complement(307..654)
                     /locus_tag="VIH70_06290"
     CDS             complement(307..654)
                     /locus_tag="VIH70_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116004.1"
                     /translation="MGKLRYIPYVISIILGIIIPIVYVNAQESLPVREDRLFEKNGIS
                     VNLDSITYTDGKILSYDLLNPPTSKVIIHDWDKLSALSKSSITTTLVGQGYTDKGSFN
                     PDPLPNSVVVTSK"
     gene            complement(642..1748)
                     /locus_tag="VIH70_06295"
     CDS             complement(642..1748)
                     /locus_tag="VIH70_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116005.1"
                     /translation="MVDNEKASMIGISAFIAVGIIILLILPQSEQKTYFLNTGTGSDS
                     NFTDTTVCLNVGTGKLIVKSSTDGNCEIKSLLGSSDISVTNDSNTITIDFNGTIAGES
                     TQCINVGTGKIIIKNSTSGNCYVKSLLATNGITITNASNTITIESHCGTTGNGTQNII
                     IVNSGCLLRSFDSGNGITLTLDGAGNIIITNNKPEQGCTSAGGTSLLKTASTCDFKGL
                     DAGLGITLTSNTNNITISKSAGYRMVASVSQNLQKTNIPTSYTDIYVSAFDDENFGSA
                     WIDCSGSTLMIISYAVAYGGSGTEQFRWVDVNNNSNVLREDNGITANQDPRTIIFSTP
                     SWCITAGGSTIEMQGKGTTGTSDPTIKGYRIWAN"
     gene            complement(1742..2071)
                     /locus_tag="VIH70_06300"
     CDS             complement(1742..2071)
                     /locus_tag="VIH70_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116006.1"
                     /translation="MIENIPFEIFSVFIGITLVLVGIGIAKQIPALILFAGMFLLTWT
                     VLIDSIDMGAIPDKSVVSGSTTTYTMKDNPFEFTDWTKVIFALFSVVLMLIGTLLVRM
                     NMVMLSW"
     gene            complement(2074..2652)
                     /locus_tag="VIH70_06305"
     CDS             complement(2074..2652)
                     /locus_tag="VIH70_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116007.1"
                     /translation="MASLIGMFMQAMIVIAILGFMSNQNPTSQQHISAIFNRLTCPLP
                     IANGVDNDLTAPGINMTYNVDIDGNPAGVNGTGNPIVGTQYICTDDPINGNLQISSAI
                     KEYGANCFSAFPCGFFGYLNDWFSGMFDKLAAFFTLILQYLSPINFNILGFQLSDLNT
                     TAQVAVLSFYAIEYIIIGISIYKIVSPYTHVG"
     gene            complement(2655..4223)
                     /locus_tag="VIH70_06310"
     CDS             complement(2655..4223)
                     /locus_tag="VIH70_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116008.1"
                     /translation="MENKKIIPLALFAIALLIFVIPERAFALQQVTVDVFNSTASNAR
                     EVEYESGTIKSWFILSSVTSTTSKLERVSATTDTLSLSKTLLLEPNHTTIPNTASGLT
                     CSTSFCYVTLQQNAPFTATWQIQKYSTSTLTLVSYYNWTLSFGGGTKPRLGDATTLYG
                     TEDCNGAPYYRLGFFALNDTTHLASFTPYGACGSSGLGTVVLHQSLANKAIERIGTTT
                     DTVFNIWRTDTGVTKCSTANIGVQSGVLYSSTLSRWVITRSTSIVLVDDSCATTVISS
                     CTSCPTFSSTWEVAENPSPGVHEIYISGATSGSTTTVIALDATSTATIGNMKFLFNVN
                     NGGTAPNSFNSRLMDSGVGTGNTGHQVGFHAVNDFVIEQIEGAPAEEGDQQQSEFCAQ
                     PENVNLLTCRLETVPALAQVSASETFGNSTLHICQQIKLCDPNNDDIKTNGLGYLLVA
                     IGLGVMTAIFWLASGGKLKDIPTFVWFIGTLCVLGAFTAFQFVDVTFFIVGILVIIAL
                     ASAKIVQNLDLGGF"
BASE COUNT         1345 a          864 c          767 g         1568 t
ORIGIN      
        1 ttttatacac tttttttcat ggtcaatatc catagacagt atagcagaat catttgcgtc
       61 aaaatagata tgacctatac ctttattgtc tgtaatcatt cttgatgttt caactgtata
      121 ttgacgttta tgccaagtga agtcttgagc atatgctttt atcttgaatg gtttacctgc
      181 ttctttattc ttgttaatca ttggatggca tacaatcttc ttggacttgt tgcctacgta
      241 aaaattgggg atttcttcag tcataggcaa ttactccata caaatactca tatataagta
      301 tgttggtcat ttagaagtca ccactactga atttggtaat gggtctggat tgaatgagcc
      361 cttatctgta tatccctgac caactaaagt tgtagttatg ctactcttac ttaatgctga
      421 tagtttatcc caatcatgga ttataacttt gctagttggc ggatttaata aatcatatga
      481 taatatcttt ccatcagtat atgttattga atccaagtta actgatattc cattcttctc
      541 gaataatcta tcttctctta ctggcaatga ttcttgagca ttaacataaa caataggaat
      601 tattattcca agtataattg aaatcacata tggaatatat cttaatttgc ccatatccta
      661 taccctttta ttgttgggtc acttgtccca gttgttccct ttccttgcat ctctattgta
      721 gagccaccag cagttatgca ccatgatgga gttgaaaata tgattgttct tgggtcttga
      781 ttagcagtaa ttccattatc ttctctcaat acgttcgaat tgttattaac atctacccat
      841 ctaaattgtt cagttccaga accaccataa gccactgcat aggatataat cattaatgta
      901 ctacccgaac agtcaatcca tgcagaacca aaattctcgt cgtcgaaagc gctaacatat
      961 atgtctgtat aactagtggg tatattagtt ttctgtaagt tttgactgac tgatgcaacc
     1021 attctatatc ctgctgattt tgatatcgtg atgttattag tattacttgt gagtgtaatt
     1081 cctagtcctg catctaatcc tttaaagtcg catgtagatg cagtttttaa gagtgatgtt
     1141 ccaccagcag aagtgcatcc ctgctctggt ttgttattag ttattatgat atttcctgca
     1201 ccatcaagtg taagagttat tccattacca gaatcaaaac tacgtaaaag gcatccacta
     1261 ttgactatta ttatgttctg tgtgccgttt ccagtggttc cgcaatgact ctctattgtt
     1321 atagtattac tggcattagt gatggttatt ccatttgtag ccaacagaga ttttacataa
     1381 caatttcctg aagttgaatt cttgattatt atttttccag ttccaacgtt tatgcattga
     1441 gtagattctc ccgctattgt cccattgaag tctattgtaa ttgtattgct atcgtttgta
     1501 accgatatat cgctagaacc caataatgat tttatttcac aattgccatc tgttgaactt
     1561 ttaactataa gtttaccagt gcctacgtta agacatactg tagtatctgt gaaattggaa
     1621 tctgaaccag ttccagtatt gaggaaatat gttttctgtt ctgattgagg taatattaat
     1681 aatattatta tccctactgc aatgaatgca gatattccaa tcattgatgc cttttcgtta
     1741 tctaccatga caacatcacc atattcattc taacaagaag tgtgcctatc agcatcaata
     1801 ctacactgaa tagggcaaat ataacttttg tccaatctgt gaattcaaat gggttatctt
     1861 tcatagtata cgtcgtagtg gaaccactta ccactgattt atcaggtata gcacccatat
     1921 ctattgaatc tatcaatact gtccaagtta acaagaacat gccagcaaat aatattaacg
     1981 ctggaatttg tttcgctatt ccaattccaa ccagaactaa tgttattcca ataaatactg
     2041 aaaatatttc aaaagggatg ttctctatca tttttaaccc acgtgagtat atggactcac
     2101 tatcttgtag atggatatgc ctattataat atattctata gcataaaatg ataatacagc
     2161 aacttgtgca gttgtattca agtctgataa ttggaatcca agaatgttaa agttgattgg
     2221 agatagatat tgtaatatca gtgtaaagaa tgcagccagt ttatcaaaca taccactgaa
     2281 ccaatcattt aggtatccaa agaatccaca tggaaaagca ctgaaacaat ttgcaccata
     2341 ttcttttatt gctgaactga tttgtagatt accgttgata gggtcgtctg tgcagatata
     2401 ttgagtccct actataggat taccagttcc atttactcca gcgggattac catcgatatc
     2461 tacattatat gtcatgttaa tacccggggc agtcaaatca ttgtctaccc cattggctat
     2521 tggtaacgga caagttaatc tattgaatat agcgcttata tgttgctgag atgttggatt
     2581 ttgatttgac atgaaaccaa gtatagcaat aactatcatt gcttgcataa acatccctat
     2641 gagggacgcc aagtttaaaa tccccctaag tctagattct gaactatctt tgcagatgct
     2701 agggcaatta tcaccaagat gccaactata aagaatgtta catccacgaa ttggaatgca
     2761 gtgaatgcac ccaatacaca tagtgttcct atgaaccata cgaatgttgg aatatctttc
     2821 aactttccac cactggctag ccagaatata gcagtcatta ctcctagacc aattgcaacc
     2881 aatagatagc ctaatccgtt agttttgata tcgtcattat tggggtcaca taattttatt
     2941 tgctggcata tatgcaatgt tgaattaccg aatgtttcac ttgcagatac ttgggctaat
     3001 gctggaacag tttccagtct acatgtaaga aggtttacat tttctggttg tgcgcagaat
     3061 tcactctgtt gttggtctcc ttcttctgct ggtgcacctt ctatttgttc tataacaaag
     3121 tcatttactg catgaaatcc tacttgatgc ccagtattgc cagttcctac accagaatcc
     3181 atcaatctgc tattgaatga attgggtgcg gttccaccat tattgacatt gaaaaggaat
     3241 ttcatattac ctattgttgc tgttgatgta gcatctaaag caattacagt cgtagtgcta
     3301 cctgatgttg cacctgaaat atatatttca tgaacacccg gagatggatt ttctgctact
     3361 tcccacgtgc tagagaatgt tggacatgaa gtgcaagaac ttattacagt tgtagcacaa
     3421 ctatcatcta ccaatactat gctagttgaa cgtgttatta cccatcttga taaagtagaa
     3481 ctatacaata ctccagattg aacaccaata ttagcagttg aacatttagt aactccagta
     3541 tcagttcgcc agatattaaa tactgtatct gtagttgtcc ctattctttc aatcgcttta
     3601 tttgctaaac tctgatgcaa tactactgtt cctaatccac tggaaccaca tgccccatat
     3661 ggagtgaatg atgctaaatg agttgtatca ttcaatgcaa agaatcctaa tctgtaatat
     3721 ggtgcaccgt tacaatcttc tgtgccatat aatgttgtag catcacctaa tctaggtttt
     3781 gttcctccac cgaatgataa agtccaatta taataactga ctaaagttaa agtgcttgtt
     3841 gaatatttct gtatctgcca tgttgcagtg aatggagcat tttgctggag tgttacataa
     3901 cagaatgaag ttgaacacgt cagtccactt gctgtatttg gaatagtagt gtgattgggt
     3961 tccagcaata atgttttaga aagagataga gtatcagttg tcgcactaac tctttctagt
     4021 ttagatgtag tgcttgttac agaacttaga atgaaccatg actttatagt tccagattca
     4081 tattctacct ctctggcatt actagccgtt gaattaaaaa cgtctactgt aacttgttga
     4141 agtgcaaatg ctctttcagg tatgacaaaa atcaatagtg ctattgcgaa aagcgcaagt
     4201 ggaattatct ttttattttc cattttctca ccttacatat tcatgaggaa aggggatttt
     4261 tcattccccg attcaggtta agcaatacag atgtcgattg tttttattct taatctctcc
     4321 tagagaagta agcgaatacc agtatgagga taatcgccac tacacctatg attggaataa
     4381 gactaaatac tgttactgtg ctatcgtccc agcctgtagt gtttgcattg aatattagac
     4441 cgaatgcgat tgggataagc actgctgtaa gcaggattgt aatgaatact ccaattacct
     4501 tttcttccat agccatttat caaccaaccc cctatatgct gtat
//
LOCUS       DATBRK010000209         8436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_753774, whole genome shotgun sequence.
ACCESSION   DATBRK010000209 DATBRK010000000
VERSION     DATBRK010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753774"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..509)
                     /locus_tag="VIH70_06315"
     CDS             complement(333..509)
                     /locus_tag="VIH70_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116009.1"
                     /translation="MDRQTAFLLALGLLILAAAIVSVTGWILFVRDRRADRPDAEGAP
                     LPSSEERGRLSADD"
     gene            complement(520..1323)
                     /locus_tag="VIH70_06320"
     CDS             complement(520..1323)
                     /locus_tag="VIH70_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116010.1"
                     /translation="MRRRRWIGAAMLVGATLGGPSTARADGGAYLDLDRTHYLPGQTA
                     HAETYVDVPRAKQHLLDRGPFYLFAVPEGVTVVEGRPIPSEAVRVATISIERERRTSF
                     ELQATFVVPELASRLYTLAVCNDPCTISGFREPLTGPISIVATTREGELLTEVDRLNG
                     RVWSLNRQVRKAERLDEESRAQLGAAEVARSELAIRVRELEAAAARTTRSARRPLISA
                     WATLILAGGLFASALALRVRRRRRLGPAVPDPIAPDPTSATAEPKVPVG"
     gene            1475..2731
                     /locus_tag="VIH70_06325"
     CDS             1475..2731
                     /locus_tag="VIH70_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116011.1"
                     /translation="MLRKLVVALSVAALGAAACTHDTQDGTASRSVPRETLLLGTGAG
                     PLVVQVPSGSVLFDRPGTVASLGGSWLLSATPSAGSTQLRIVDGADAEPASTVRLDRT
                     LDVRVVAESGAAVALMDPLPDGWDPEVPLPRSRTPIVVADPTGTMEPRTYDLRGNFEP
                     EAFSTDDRRLFLIQHLPAEAPTVYRVTVLNLRTGRVLPVFGPFKGPAERMPGIRLQQV
                     LSPNADQLYTLYSSARPGYAPHHAPVPNGATVSFVHVLSLQDGWAHCVGLPEELWDRP
                     ASEQAMATSPDGRLLYIVDPTLGLVTVMDTASLEIRTSTIDLAVGGVRHTSAAVSPDG
                     STLFVGTAGERGTISRIDTDTLGLVRTWTTEGVSGLGLSADGERLYVAFDDRVEVMDA
                     RTGSELAGVAVASPAPIERVSVALAS"
     gene            2790..3869
                     /locus_tag="VIH70_06330"
     CDS             2790..3869
                     /locus_tag="VIH70_06330"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY8116012.1"
                     /translation="MRLPVMPPVGPMLAKSIKEIPAGPFSYEPKWDGFRSIVFRDRDE
                     VEIGSRNERPMTRYFPEVVEAIMTNLPERAVVDGEIVVPDPGLGRLEFETLQQRIHPA
                     DSRVRMLARATPAHLVAFDLLALGDDDLTGRPFAERRAALEVALADATSPIHVTPATT
                     DIEVALRWFDEFEGAGLDGVVAKPLEGTYQQDKRVMFKIKHERTADCVVAGYRLHKSG
                     PDAVGSLLLGLYDDAGVLANVGVIGAFPMDHRRKLLEELHPWVTTFDGHPWDWEQEMA
                     DARTPRSAEGSRWNPKKDLSFVPLRPERVVEVRYDHMEGNRFRHTAQFVRWRPDREPR
                     SCTFEQLEEPVSFDLAEILGVAPKG"
     gene            complement(3859..5469)
                     /locus_tag="VIH70_06335"
     CDS             complement(3859..5469)
                     /locus_tag="VIH70_06335"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY8116013.1"
                     /translation="MAGWFVHEDFTDEERTRLEPHFTNLDGPVFALTNLPEVVKGALF
                     ARYSRTPKSLRRLFLDEFAEPLEAGAVAGLEGVRVDRAERLFDRVFFEFGDDSVAQLG
                     GVHLACEQASQLLAKAIEWGRLAAYLEQSTRYMRYDDRPGGRWRLTTPPEIEGGPAGE
                     AYEAYATRTFDAYGRMFRPLFDLFAERFPKDPDDSDFLYRSTITAKTCDTLRVLLPAG
                     TRSNLGVFATGQAYEQMLMRMHAHPLAEVREYADLMLVELRKVIPAFLKRVDLPDRGV
                     AWSRYLAETREATAEVTSKFTEGAEPGHRDAVTLTDYDPLGEEKVIAAAMYAASDLPD
                     DKLQSLAHSMSADERDAVLRAYVGERGNRRHKPGRAFERTGYRFDVLCDYGAFRDLQR
                     HRLLTLEWQRLSPQHGFDTPEVIADARMTDEWNRVMEDSAATWATLADQAGPEVAQYA
                     VSMAYRIRFVLQMSAREAMHLIELRSSPQGHPTYRRIAQRMHELIDTTAGHRAIASTM
                     TYLDSSDVDLERLEAERRAEAKRKAATP"
     gene            complement(5472..7166)
                     /gene="pknB"
                     /locus_tag="VIH70_06340"
     CDS             complement(5472..7166)
                     /gene="pknB"
                     /locus_tag="VIH70_06340"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEY8116014.1"
                     /translation="MTLGGRYRVERELGRGGMAKVFLGTDTVLGRTVAVKVLAPQFAD
                     DDGFVMRFRREAQAAASIGHPHIVSVFDTGSDDGVHFIVMEYVEGRTLAEFLAGGGRI
                     LPDRAIDIAMDVCGALEAAHARGVIHRDIKPGNIMLNPRGEVKVTDFGIARVTATADT
                     IEQTAAILGTASYLSPEQAQGQPVDGRSDIYSLGCVLYEMVTGRPPFLGDSPVAVASK
                     QVLEQPAPPSRLNSDVTADLDAVILRALAKNPENRYQSAEEMRADLERAKRGLPVQAT
                     PLLAAGTTQVLNPPPAHATEVLPPPERERGTNWVPIVVTLVLIALLGALLWFLAANVL
                     NNDDQQTGVMVTVPNVVGDKRAQAISKLEDAGLEVGTITTVPAADDTQEPGTVLEQDP
                     AAGGEVDRGTKVDLTIVAAPDQVAIPDLEGSSVEEAQATLVDLGLEPGGPVEEPSDTV
                     PDGSVTRTDPASGQEVEPGSTVTIFVSTGAAQVAVPEVRCQSFGSAQNELSKLGLNSV
                     ISSDTVAVNTACPLGNKVAEQDPAPGTQVDPGTTVMLFEGAEGPSGPTGATGGTGE"
     gene            complement(7195..>8436)
                     /locus_tag="VIH70_06345"
     CDS             complement(7195..>8436)
                     /locus_tag="VIH70_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8116015.1"
                     /translation="AELYAHETGFYSFVFGRTELEQSYNDFLTGDAAELLPQTFTDLI
                     LGRPKQGATIVTTLVPEIQEAAAAAAQAEAGDVAIAAIDPATGDVLALVSEPSYDPNL
                     LASQDPKVVRDSWDELNADPEKPLLSRASDELFPPGSTFKLVTASAALENGFGPESLW
                     PNPNELDLPLTDATIENFGGSTCSGGSQITLADALRQSCNVVFGAVGLELGADRLAEQ
                     ARAYGFTAEAGEDLVPFDIPWSSGVFPAPETFEGRDPAVAISAIGQQDVAANPLQMAL
                     VGAAIANDGVEMQPRLATEARDPSGRVIAEFKPQEFSRPLSPENAAALTQMMVGVVES
                     GTGTAAQIPGVTVAGKTGTAQHGEDENPHAWFVCFAPAEAPEVAVAVIVLDGGSLGSE
                     ATGGQVAAPIARAVVEAALGV"
BASE COUNT         1185 a         2992 c         3007 g         1252 t
ORIGIN      
        1 aggcgctcgg cgccgagccg ggccagggtg tgctcgacgg tgatcgaacc gcggaggccg
       61 atcacgtctt cggccgagaa gggacgctcg atgccacccc atcgagggca cgaggaccag
      121 tgcgtgcgga ggttgtcggc ctctcgccgg atccgctctt cgtacgtgag atcgatcatg
      181 ccgcccctcc ttaccctcgc gggccggtcg gataacgaac gaccctcgcg gctgcgaggg
      241 tcggaagggg cgcctgagca ggccggatcc ggccgctcac gggcccctac gggcgggata
      301 cgcggaaacg cgaggcgagc ttgaaccgtc gttcaatcat cggccgagag tctcccacgc
      361 tcctcgctcg agggcaaggg cgccccctcc gcgtccgggc gatcggcacg gcggtcgcgg
      421 acgaacagga tccagccggt cacggagacg atcgcggcgg cgaggatcag cagccccagg
      481 gccagcagga acgcggtctg tcggtccacc gcccgaaggc tacccgaccg gaaccttcgg
      541 ctcggcggtg gccgaggtgg ggtccggggc gatcgggtcg gggaccgccg gcccgaggcg
      601 tcgcctgcgc cggaccctga gcgcgagcgc cgaggcgaac aacccaccgg ccaggatcag
      661 cgtcgcccac gccgagatca acggacgccg ggccgatcgg gttgttctcg cagcggcggc
      721 ttcgagctca cgaacacgga tggccagctc ggagcgggcg acctcggcgg cgccgagctg
      781 ggctcggctc tcctcgtcca ggcgttcggc cttgcggacc tgccgattca ggctccacac
      841 ccggccgttc aaacggtcga cctccgtgag cagctcgccc tcgcgggtgg tggcgacgat
      901 cgagatcggt ccggtgagcg gttcccggaa ccccgagatc gtgcacggat cgttgcagac
      961 ggcgagcgtg tacaagcggc tcgccagctc ggggacgacg aacgtcgcct ggagctcgaa
     1021 cgacgtgcgc cgttcgcgtt cgatggagat ggtggccacc cggaccgcct ccgaggggat
     1081 cggccggccc tcgaccaccg tgacgccttc gggcaccgcg aacaggtaga agggtccgcg
     1141 atccaacagg tgctgcttgg cgcgtgggac gtccacgtac gtctcggcgt gcgcggtctg
     1201 gccgggcagg tagtgcgtcc ggtcgagatc caggtacgcc ccgccatcgg cccgcgccgt
     1261 cgaaggccct cccagcgtcg ccccgacgag catcgcggcg ccgatccatc gacgtctgcg
     1321 catgcgaccc ctcctcgatc cggtcagcca ccgagcagac ccgccggtgg ctccgccggt
     1381 tcccgggaac cgtgcggtcc cccgaggagt agttgtcatc gaaggcttgg acgtacgcga
     1441 gacgtgctcg atgagccgaa cggggaggtc ccagatgctg aggaagctgg tcgtggcact
     1501 gtccgtggcg gccctgggcg ccgccgcctg cacgcatgac acccaggacg ggacggcgag
     1561 ccgatccgtg cctcgcgaga ccctgctcct gggcacgggg gcgggaccgc tggtcgtcca
     1621 ggtcccgagc gggtcggtac tgttcgatcg cccggggacc gtcgcgagcc tcggcggctc
     1681 gtggctgctc tcggccaccc cctccgcggg ctccacgcag ctccggatcg tcgacggcgc
     1741 ggacgcggaa ccggcctcca ccgtccggct ggaccgcacc ctcgacgtcc gggttgtcgc
     1801 ggagagcggt gcggcggtcg cgctgatgga cccgctgccc gacggctggg acccggaggt
     1861 gccgctcccg cgttcgagga cgccgatcgt ggtggccgac ccgaccggaa cgatggagcc
     1921 tcggacctac gacctgcgtg ggaacttcga gcccgaggcg ttctcgaccg acgaccggcg
     1981 gctgttcctg atccagcacc tgcccgcgga ggcgccgacc gtctaccgcg tcaccgtgct
     2041 caacctgcgc accggacggg tgctgccggt gttcgggccg ttcaagggcc cggccgagcg
     2101 gatgccgggg atccgtctgc agcaggtgct ctcgccgaac gccgaccagc tgtacacgct
     2161 ctactcgagc gcgcggcccg ggtacgcgcc gcaccacgcc cccgtgccga acggcgcgac
     2221 ggtgtcgttc gtccacgtgc tcagcctcca ggacgggtgg gcgcactgcg tcgggctgcc
     2281 cgaggagcta tgggaccggc ccgcgagcga gcaggcgatg gcgacctcgc ccgacggccg
     2341 gctcctctac atcgtcgacc cgacgctcgg cctggtcacc gtcatggaca cggcgtcgct
     2401 cgagatccgg acgagcacca tcgacctcgc ggtcggcggc gtccggcaca cctcggccgc
     2461 cgtcagcccg gacggttcga cgctgttcgt gggaacggcg ggagagcgcg gcacgatctc
     2521 gcggatcgac acggacaccc tcggtctggt ccggacctgg accaccgaag gcgtgtcggg
     2581 cctcgggctc tcggccgacg gggagcgcct gtacgtcgcg ttcgacgacc gcgtcgaggt
     2641 gatggatgcg aggaccggtt cggagctggc cggcgtcgcc gtcgcgagcc ccgcgccgat
     2701 cgaacgcgtg agcgtggccc tggcctccta gggacggggc gccgcgcgcc cgcgggccgg
     2761 gagagcgccg gtcgggcaag ataggtggca tgcgtctgcc cgtcatgccg cccgtcggcc
     2821 ccatgctcgc gaagtcgatc aaggagatcc ctgccggccc gttcagctac gagcccaagt
     2881 gggacgggtt ccgctcgatc gtcttccgcg accgcgacga ggtggagatc ggcagccgca
     2941 acgagcggcc gatgacccgc tacttcccgg aggtcgtcga ggcgatcatg acgaacctgc
     3001 cggagcgggc cgtcgtcgac ggcgagatcg tggtccccga ccccgggctg ggccggctcg
     3061 agttcgagac gctccagcag cggatccatc cggccgacag ccgcgtccgg atgctggccc
     3121 gggccacccc ggcccatctc gtcgcgttcg atctgctcgc gctcggcgac gacgacctca
     3181 cgggtcgacc gttcgccgag cggcgggcgg cgctcgaggt cgcgctcgcg gacgcgacgt
     3241 cgccgatcca tgtcacgccg gccacgaccg acatcgaggt cgcgctgcgg tggttcgacg
     3301 agttcgaggg ggcgggactc gacggagtgg tggcgaagcc gctcgagggg acctaccaac
     3361 aggacaagcg cgtgatgttc aagatcaagc acgagcgcac cgccgactgc gtcgtggccg
     3421 ggtaccggct gcacaagagc ggccccgatg ccgtcgggtc gctcctgctc gggctgtacg
     3481 acgacgcggg cgtcctcgcc aatgtgggcg tgatcggcgc gttcccgatg gaccatcgac
     3541 ggaagctcct cgaggagctg catccctggg tcaccacctt cgacgggcac ccgtgggact
     3601 gggaacagga gatggccgat gcccggacgc cgcgcagcgc cgagggtagc cgctggaacc
     3661 cgaagaagga cctttcgttc gtcccgctca gacccgagcg ggtcgtcgag gtccgctacg
     3721 accacatgga ggggaatcgg ttccggcaca cggcgcagtt cgtcaggtgg cgccccgacc
     3781 gcgaaccgcg ctcgtgcacg ttcgagcagc tggaggagcc ggtgagcttc gacctggccg
     3841 agatcctggg cgtcgcacct aaggggtagc ggccttgcgt ttcgcctcgg cgcggcgctc
     3901 ggcctcgagg cgttcgagat cgacgtcgct cgagtcgagg tatgtcatcg tcgaggcgat
     3961 cgctcggtgg ccggccgtcg tgtcgatcaa ctcgtgcatc cgctgcgcga tgcgccggta
     4021 ggtcgggtga ccctgcggcg acgaccggag ctcgatcagg tgcatcgcct cccgggcgct
     4081 catctgcagc acgaaccgga ttcggtacgc catcgagacc gcgtactgcg ccacctccgg
     4141 acccgcctgg tccgcgagcg tggcccaggt cgcggccgag tcctccatca cgcggttcca
     4201 ctcgtcggtc atgcgcgcgt cggcgatcac ctccggtgtg tcgaacccgt gctggggcga
     4261 caggcgttgc cattcgagcg tgagcaggcg gtgacgctgc aggtcgcgga acgcgccgta
     4321 gtcgcacagc acgtcgaacc ggtagccggt gcgttcgaac gcgcggccgg gcttgtgccg
     4381 ccggttgccg cgttcgccta cgtaggcgcg caacaccgcg tcgcgctcgt cggcgctcat
     4441 cgagtgcgcg agcgactgta gcttgtcgtc gggcaggtcc gacgccgcgt acatcgccgc
     4501 ggcgatcacc ttctcctcgc caagcgggtc gtagtcggtg agcgtgacgg cgtcgcgatg
     4561 gcccggctcg gccccctcgg tgaacttcga cgtgacctcg gccgtcgcct cgcgggtctc
     4621 cgcgaggtac cgggaccagg ccacgccgcg atcggggagg tcgacgcgct tgaggaacgc
     4681 ggggatcacc ttacgaagct cgacgagcat caggtcggcg tactcgcgca cctcggcgag
     4741 ggggtgggcg tgcatccgca tcagcatctg ctcgtaggcc tggccggtcg cgaacacgcc
     4801 gagattcgaa cgcgtcccgg ccggcagcaa cacccggagc gtgtcgcagg tcttcgccgt
     4861 gatcgtcgag cggtacagga agtccgaatc gtccgggtcc ttggggaagc gctcggcgaa
     4921 caggtcgaac agcggccgga acatccgccc gtacgcgtcg aacgtccgcg tcgcgtacgc
     4981 ctcgtaggcc tcgccggcgg ggccgccctc tatctcgggc ggcgtcgtga gccgccagcg
     5041 gcctccgggc cggtcgtcgt agcgcatgta gcgcgtcgac tgctcgaggt aggccgcgag
     5101 gcgcccccat tcgatcgcct tcgcgagcag ctgcgatgcc tgctcgcacg cgaggtgcac
     5161 gccgccgagc tgcgcgacgg agtcgtcgcc gaactcgaag aagacgcggt cgaacagccg
     5221 ctcggccctg tcgaccctga cgccctcgag ccccgcgacg gcgccggcct ccagcggctc
     5281 ggcgaactcg tcgaggaaca ggcggcggag cgacttcggc gtgcgcgagt agcgggcgaa
     5341 cagggcgccc ttgaccacct cgggcaggtt cgtgagcgcg aagaccgggc cgtcgaggtt
     5401 cgtgaagtgg ggttcgagcc gggttcgttc ctcgtcggtg aagtcctcgt ggacgaacca
     5461 gcccgccacg cctactcgcc ggtcccaccg gtggcgccgg tcgggccgct gggcccctcc
     5521 gcgccctcga acagcatcac ggtcgtgccc gggtcgacct gggtgcctgg cgcgggatct
     5581 tgctcggcca ccttgttgcc gagcgggcac gcggtgttga ccgccacggt gtcgctcgag
     5641 atgaccgagt tcaggcccag cttggagagc tcgttctggg ccgagccgaa gctctggcac
     5701 cgcacctcgg ggacggcgac ctgcgcggct ccggtcgaga cgaagatggt caccgtcgat
     5761 cccggctcga cctcctgccc cgaggcgggg tcggtccgtg tcaccgaacc gtccgggacc
     5821 gtgtcgctcg gctcctcgac cgggcccccc ggttccagac cgaggtcgac cagcgtggcc
     5881 tgggcctcct ccaccgacga cccctcgagg tccgggatcg ccacctgatc cggcgccgcg
     5941 acgatcgtga ggtcgacctt cgtgccgcgg tcgacctcgc cgcccgcggc ggggtcctgt
     6001 tccaggaccg tgccgggctc ctgggtgtcg tcggcggccg ggacggtggt gatggtgccg
     6061 acctcgagtc cggcatcctc gagcttgctg atcgcctggg cccgtttgtc gccgaccacg
     6121 ttcgggacgg tgaccatcac ccccgtctgc tgatcgtcgt tgttcaggac gttggccgcc
     6181 aggaaccaca gcagggcccc caggagcgcg atcagtacga gcgtgacgac gatgggcacc
     6241 cagttcgtgc cgcgctcgcg ttcgggcggc ggaagcacct cggtcgcatg cgcgggtggc
     6301 ggattcagga cctgggtcgt ccccgcggcg agcagcggcg tcgcctgcac ggggagcccg
     6361 cgcttggcgc gttcgaggtc ggcgcgcatc tcctcggccg actggtagcg gttctccggg
     6421 ttcttcgcga gcgcccgcag gatcacggcg tcgaggtcgg ccgtgacgtc ggagttcaac
     6481 cgcgacggcg gcgccggctg ctccaggacc tgcttggacg cgaccgcgac gggggagtcg
     6541 ccgaggaacg gcggccggcc cgtgaccatc tcgtacagca cgcatccgag cgaatagatg
     6601 tccgaacgcc cgtcgacggg ctgtccctgc gcctgctccg gcgacaggta ggacgccgtg
     6661 ccgaggatcg ccgcggtctg ctcgatcgtg tcggccgtcg cggtgacccg tgcgatgccg
     6721 aagtcggtga ccttcacctc gccgcgcggg ttgagcatga tgttgccggg cttgatgtcg
     6781 cggtggatca cgcctcgcgc atgcgcggcc tccagcgccc cgcacacgtc catcgcgatg
     6841 tcgatcgcgc ggtcggggag gatgcgccca ccccccgcga ggaactccgc gagcgtccgg
     6901 ccctcgacgt actccatcac gatgaagtgc acgccgtcgt ccgagccggt gtcgaacacc
     6961 gagacgatgt gcggatgccc gatgctcgcc gcggcctgcg cctcgcgacg gaaccgcatc
     7021 acgaagccat cgtcgtcggc gaactgcggt gcgaggacct tcacggccac cgtccggccg
     7081 agcaccgtgt cggtgcccag gaacaccttg gccatgccgc cccggccgag ctcacgctcg
     7141 acgcggtagc gtccccccag ggtcaccggc aggtcgatct tcggtacctc ctgctcacac
     7201 gcccaaggcg gcctcgacga ccgccctcgc gatcggggcg gcgacctggc cgcccgtcgc
     7261 ttcgctgcca aggctacccc cgtcgaggac gatcacggcg acggccacct cgggagcttc
     7321 ggcgggcgcg aagcacacga accacgcgtg cgggttctcg tcctcgccgt gctgggcggt
     7381 gccggtcttg ccggccaccg tgacacccgg gatctgggcc gccgtgccgg tcccactctc
     7441 gacgacgcct accatcatct gcgtcagcgc cgcggcgttc tccggcgaca gcggccgcga
     7501 gaactcctgc ggcttgaact ccgcgatcac gcgcccgctt gggtctcggg cctcggtggc
     7561 caggcgcggc tgcatctcga cgccgtcgtt ggcgatcgcc gccccgacca gggccatctg
     7621 cagcgggttc gcggcaacgt cctgctgtcc gatcgccgag atcgcgacgg cgggatcacg
     7681 gccctcgaac gtctcgggcg cggggaacac ccccgagctc cacgggatgt cgaacggcac
     7741 gaggtcctcg ccggcctcgg cggtgaaccc gtatgcgcgc gcctgctcgg cgagcctgtc
     7801 ggcgccgagc tcgagcccca ccgcgccgaa cacgacgttg cacgattgac ggagcgcgtc
     7861 ggccagcgtg atctgcgagc ctcccgaaca cgtcgacccg ccgaagttct cgatcgtcgc
     7921 gtcggtgagc gggaggtcga gctcgttcgg attgggccac aggctctccg ggccgaaccc
     7981 gttctcgagc gcggccgacg ccgtcacgag cttgaaggtc gagccgggcg ggaacagctc
     8041 gtcgctcgcg cgcgagagca gcggcttctc gggatcggca ttcagctcgt cccacgaatc
     8101 ccgcacgacc ttggggtcct gcgaggcgag caggttcggg tcgtagctcg gctcggagac
     8161 cagggcgagc acgtcgcccg tcgcggggtc gatcgcggcg atcgcgacgt cgcccgcctc
     8221 cgcctgcgcc gcggccgcgg cggcctcctg gatctcgggc acgagggtcg tcacgatcgt
     8281 cgcgccctgc ttgggcctgc cgaggatcag atcggtgaac gtctgcggca ggagctcggc
     8341 cgcgtcgccg gtcaggaagt cgttgtagct ctgctcgagc tccgtgcgac cgaacacgaa
     8401 cgagtagaag cccgtctcgt gcgcgtacag ctccgc
//
LOCUS       DATBRK010000210         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2344977, whole genome shotgun sequence.
ACCESSION   DATBRK010000210 DATBRK010000000
VERSION     DATBRK010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2344977"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VIH70_06350"
     CDS             complement(<1..128)
                     /locus_tag="VIH70_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY8116016.1"
                     /translation="MVSAFILIQTEVGRASNVTREISGVKGIVSVDPVTGPYDVIAR"
     gene            complement(204..1412)
                     /locus_tag="VIH70_06355"
     CDS             complement(204..1412)
                     /locus_tag="VIH70_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8116017.1"
                     /translation="MRADGARRIRPAVAVQTLFVLFGVFIAASFPFLSLFLDGKGLTA
                     GEIGAVIAAMAIARVLLSPLWGHLADTTLGRRRALQIGALGGAIGAFALFGAQTYLAI
                     VGAAFVFAGLSATVAPNVDAIALEFLGDDRMTEYGRIRGWESLAYATACLLIGVLLQQ
                     VGIRWAMPVVASAALAIFVWAAVAIEPDRPARSIRHGRLGAVGAVFRAAPRFWMFLLA
                     ILLVWTGFNAAWNFIGLKIERAGGGAMLVGIGTALGGMVEVPVMRLSSRFSNRFGLRA
                     VYVAGCVVYATGFLLWGLVEDPTILSVLTVFEGAGFALLFTTMVVVIGRMLPSNLYST
                     GQSLAATMGFGIAPIIGAGLGGLVFGRFGPATLYIGACLLTLCGGAVAWVALSTPALS
                     RPARDVEPVL"
     gene            complement(1461..1961)
                     /locus_tag="VIH70_06360"
     CDS             complement(1461..1961)
                     /locus_tag="VIH70_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8116018.1"
                     /translation="MGSPERIRLVSREEWERVRDLRLRALADAPDAFGSTLDLERGNG
                     PAEWIGWIEGWEGATNAMFVAERGDRWIGMAVGSRTGEEDHAHLYGMWVDPMWRGAGV
                     GARLVEGVLGWAWSWGARWVGLGVTESNDGATGFYENLGFVDTGERYPLREGSRLTCR
                     TMRREP"
     gene            2023..>2207
                     /locus_tag="VIH70_06365"
     CDS             2023..>2207
                     /locus_tag="VIH70_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8116019.1"
                     /translation="MPNDDAAWWRDGVLYQIYVRSFADSDGDGTGDLQGVIEHLDHLE
                     WLGVDGIWLSPTTPSPDR"
BASE COUNT          373 a          830 c          735 g          269 t
ORIGIN      
        1 cgggcgatga cgtcgtaggg accggtcacg gggtcgacgg acacgatgcc cttcacgccg
       61 ctgatctccc gggtcacgtt gctggcccgt cccacctcgg tctggatcag gatgaacgcg
      121 ctgaccacgt cgacctcctc gtcagggtct cggggcatcg tatgtcaagc cccgaggcgg
      181 cgcgaaccga ccgagcacgg ccctcagagg acgggttcga cgtcccgtgc ggggcgggac
      241 agggcgggcg tgctcagcgc gacccacgcg acggcccctc cacagagcgt gaggaggcag
      301 gcgccgatgt acagcgtcgc cgggccgaag cgaccgaaca cgagcccgcc gagccccgcg
      361 ccgatgatcg gcgcgatccc gaaccccatc gtcgcggcga gcgactgacc ggtcgagtag
      421 aggttcgacg ggagcatgcg gccgatcacg accaccatcg tcgtgaacag gagcgcgaac
      481 ccggcgccct cgaacacggt gaggacggac aggatcgtcg ggtcctcgac cagaccccac
      541 agcaggaacc ccgtcgcgta cacgacgcac ccggccacgt acacggcccg caggccgaaa
      601 cggttcgaga accgcgatga gagccgcatc acggggacct cgaccatccc gccgagcgcg
      661 gtcccgatcc ccaccagcat tgcgccgcct ccggcgcgtt cgatcttcag cccgatgaag
      721 ttccacgccg cgttgaaccc ggtccacacc aggaggatgg cgagcaggaa catccagaac
      781 ctcggggccg cccggaacac ggcgcctacc gcgccgagcc ggccgtgccg gatcgagcgg
      841 gccggccgat cgggctcgat cgcgacggcc gcccacacga agatggcgag cgccgccgag
      901 gcgacgaccg gcatcgccca ccggatgccg acctgctgca ggagcacgcc gatcagcagg
      961 cacgccgtgg cgtacgccag gctctcccat ccgcggatgc gcccgtactc ggtcatccgg
     1021 tcgtcgccca ggaactccag tgcgatcgcg tcgacgttcg gcgcgacggt ggcgctcagg
     1081 ccggcgaaca cgaacgccgc cccgacgatc gcgaggtagg tctgcgcccc gaagagcgcg
     1141 aacgcgccga tcgcaccgcc cagcgccccg atctgcagcg cgcgacgccg gccgagcgtc
     1201 gtgtcggcga ggtgacccca gagcgggctc aggagcacgc gcgctatcgc catcgcggcg
     1261 atcaccgcgc cgatctcgcc cgcggtgagc cccttgccgt ccaggaacag ggacaggaac
     1321 gggaacgagg cggcgatgaa gacgccgaag agcacgaaca gcgtctgcac ggcgaccgcg
     1381 ggacggatcc gtcgagcccc gtccgcccgc atcgagcaac ggtaacaggg gttctgcgag
     1441 ggccatccgg cctgacgtcg tcagggttcg cgacgcatgg tgcggcaggt cagtcggctc
     1501 ccctcgcgca gcgggtatcg ctcaccggtg tcgacgaacc ccagattctc gtagaagccg
     1561 gtcgctccgt cgttgctctc ggtgaccccc aggcccaccc agcgcgcgcc ccacgaccac
     1621 gcccacccca agacgccctc caccaaccgc gcgccgaccc ccgcgcctcg ccacatcggg
     1681 tccacccaca tcccgtagag gtgggcgtga tcctcctcgc cggtgcgcga acccacggcc
     1741 atcccgatcc atcgatcacc gcgctcggcg acgaacatcg cgttcgtcgc cccctcccag
     1801 ccctcgatcc agccgatcca ctcggccggg ccgttgcccc gctccagatc gagcgtggag
     1861 ccgaaggcat cgggcgcgtc ggcgagggcg cgcaggcgca ggtcgcggac gcgctcccac
     1921 tcttcgcgcg agaccagcct gatccgctcg ggcgatccca tcccacgcat ggtagtgggc
     1981 gggggttcgg gcggccgcga tacttgggcg atgcttgggg cggtgccgaa cgacgatgcc
     2041 gcgtggtggc gggacggggt cctgtaccag atctacgtcc gatccttcgc cgacagcgac
     2101 ggcgatggca ccggcgacct gcagggcgtg atcgaacacc tcgaccacct cgagtggctc
     2161 ggcgtggacg ggatctggct ctcccccacc acgccgtccc cggatcg
//
LOCUS       DATBRK010000211         6693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_753967, whole genome shotgun sequence.
ACCESSION   DATBRK010000211 DATBRK010000000
VERSION     DATBRK010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753967"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            659..967
                     /locus_tag="VIH70_06370"
     CDS             659..967
                     /locus_tag="VIH70_06370"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8116020.1"
                     /translation="MPHRGAGLGWVSEHYRAQGVRFLGVDERDDQAAARAFVEEFGWR
                     YPSASDPAGSLADDYRLIGLPTTSIIDPSGTIRYRFQGYLDRDVLRSTLDDVLSGSDR
                     "
     gene            964..1689
                     /locus_tag="VIH70_06375"
     CDS             964..1689
                     /locus_tag="VIH70_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY8116021.1"
                     /translation="MSALAPFALAFVAGIVSFTSPCCLPLMPGYVSYVSGVAGDGSTA
                     MLVRSRTMLSATLFVSGFAATFTLMGAAASGLGSLILYHRLVLGRVAGSFVIAMGLAT
                     MGLLRVPFLYKERRFDLARIRPGPVGAVPLGMAFAIGWTPCIGPVLAAILTAAATVQD
                     AWRGAALLFVYSLGLGVPFLLLAFAVARTGRLFAWLRQHGRAIEVGGGILVLMGVLMI
                     TGQWVQLFAPLLRLFSRTGWPPV"
     gene            1686..3752
                     /locus_tag="VIH70_06380"
     CDS             1686..3752
                     /locus_tag="VIH70_06380"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEY8116022.1"
                     /translation="MRRLALLVVLAALTLCLTPSVAWAHAGFVSSSPEPGSQVSSAPG
                     VVTLRFSEPLNTKLSRAAVTTPDGRSVEGTVTSSEQIAIPLSTNAQGVYTVDWTTVSL
                     VDGHTLTGSFRFGVGVSPGAGAEGGTSDEPNGSGYLIAIGRLVEDTSLLLLLGLLLLG
                     RLARREPRLAWVRTPVQPVFLAAFAGGLAVVLGEALVAAPSPSGSAILTYLTSGLPGW
                     SRLGRVVLEGLGVLTAWRWPRARTPLAVAAVVALAAAGHAAAIQPRAWGVTVEAVHLL
                     SAGVWAGGILALTLQRPPGGLLGIDGRVLLDRFTPAALTAFAVTVATGLIRGVQEVGS
                     WGDLFGSSYGVVLLVKVLLVVLMVQFSVLAWRRIAVFPRGETAAAILVIGAAALLSAY
                     PLPPARQVEAAEEAEPTAQASPIPAGAALTLGSHAGPVLVGLTVEPATPGRNELTIYV
                     QSLDGPDATASLPVHASVDGDDVALTQCADTCRRGTANVEGGEVVVVDVGTASGGQAT
                     FYLPDLPVAPGDRVLQRMLGTMGSLTSYRLDESLTSGLETTVRTTYAFAAPNSFESHV
                     VESGSTFSTVWIGDTRYTREGNGVWKVERGAPAPPVPTYIWDSFQPYRDARILGSAQV
                     NGVRTTELAFAGGDQDLPVWFRLWVDAQGYVHRAEMRAPGHFMDHRYYDYDVPITIQP
                     PKGASG"
     gene            3761..4315
                     /locus_tag="VIH70_06385"
     CDS             3761..4315
                     /locus_tag="VIH70_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3959..3961,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEY8116023.1"
                     /translation="MLVAAGLTLGLVAAACSRQPTSSGQGPIAVGRTAPAFSLKSASG
                     GTVSLSEFAGKPVLLYFSMGPGUGGCMQQVVDLQRDKAFEDLGVRLLAISPDPVSAWQ
                     QEGGSLGITVPTLSDPGNTVWTKYGTPGWLMATNEPGHTFFLVGPDGKIAWLRDYGAP
                     EHGGAMYVEPDEIVSQVQQALSGS"
     gene            4312..5646
                     /locus_tag="VIH70_06390"
     CDS             4312..5646
                     /locus_tag="VIH70_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEY8116024.1"
                     /translation="MRRALGLLAAASVFGALAHALDIAFGMTWEILWALVLGFVLSAA
                     VQAVVSKSEMTRLLPDDSPRTIAIACGLGAASSSCSYAAVALARSIFRKGANFTAAMA
                     FEFASTNLVIELGIILWLLMGWQFTLAEFVGGPIMIAFLTVVFRFTLTHERVEEARVQ
                     ADRGLVGSMEGHAAMDMSVSGGSIFERLTSPRGFTAISHYYVMDWASIWKDVAGGLLI
                     AGALAAWVPESFWQSFFLVDHPLLAKIWGPLVGPIVAMLSFVCSIGNVPLAAVLWNGG
                     ISFGGVVSFIFADLIVLPILNIYRKYYGRRVSLYLLVTFYAAMVVAGLVVETLFGILG
                     LVPEERNAKVVEASVTWNYTTILNIAFLTMTLVLVVRFLRTGGPAMLRMMNRPAEELV
                     APAPEGGRHMSEHDEDATEYTCPMHPEVSSPTPGRCPKCGMDLVPRREDNDH"
     gene            5842..6057
                     /locus_tag="VIH70_06395"
     CDS             5842..6057
                     /locus_tag="VIH70_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007027259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY8116025.1"
                     /translation="MSGWMWDGGWMWLWMVFMVLVWAALIVFGVWAVRRFTDRSSGSA
                     RRILEERFARGEIDADEFQRQRDLLGR"
     gene            complement(6072..6485)
                     /locus_tag="VIH70_06400"
     CDS             complement(6072..6485)
                     /locus_tag="VIH70_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116026.1"
                     /translation="MSVGTDQIHDFNPGIAPSGLFWTVALPFGSVRVGGLSGTASMNA
                     TDLEIDDYFTIENALFGGGPEEVPATASFDVEWSGVTGEGSGSDTTNDFTLEFVTAGT
                     TMGWTIESGGATYATTGMTATPFAIVGKERNGIFF"
     gene            complement(6501..>6693)
                     /locus_tag="VIH70_06405"
     CDS             complement(6501..>6693)
                     /locus_tag="VIH70_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116027.1"
                     /translation="SEPSTITDFNGSVGVSVTQGTWSATGTPTGGVSSGVWEADMRFM
                     KGLFVGTDGRRRQGAFGFV"
BASE COUNT          954 a         2214 c         2325 g         1200 t
ORIGIN      
        1 atggccatgg cgccatgccc cgactgccag gctcccgggc cgcaggtcct ctcagtctgc
       61 attgccgccc tggccgcgac ggtcacgctg ttcctctcgg tgttcgccct ccgcatcgag
      121 ccacggtcgc gatcgatgcc gctgctgctg atcgcccgag ccctcgaacg acccctcgcg
      181 cagcctgatc ggcatctagc tctcggccga agccgagggc cattcgcgct gcgatagatc
      241 ggcaggactc ctgggctgcg tcgcaccagg catctcctcg tgagtcgccg gcgctaggag
      301 gcgaggaggg tcgggaattg agtagttcct catccaggac gaagcgtcga cgagtgcctc
      361 gagaggctaa tgcgggcgcc gggcaagtca gccagacgac gcgagcggtg gggacgtggc
      421 tcgtcgcgct caccgcggtc gcaatcgtcg taaccctcgc gactgtcgag tttcgcaccc
      481 gagggaccgg cgacatcggc ggcttcaccg tcgcgaacta caaggcgagg gcggaggtgg
      541 aaagcacacc ggcacccgag ttcggcctgc cgtcgctcga cggtggagag cctgtcggcg
      601 tctcgtcttt tcgcaggcaa ctagcggtcc tgaacttctg ggcctcgtgg tgcttcccat
      661 gccgcaccga ggcgccgggc tcgggtgggt atccgagcac taccgagcgc agggggtccg
      721 gttcctcggc gtggacgagc gcgacgatca ggcggccgcc cgagcgttcg tcgaggagtt
      781 cggctggcga tacccgagcg cctcggatcc cgccggttcg ctcgccgatg actaccggct
      841 gatcggtctt ccgacgacgt ccatcatcga tccgagtggg acgatccgtt accggttcca
      901 gggatacctg gatcgagacg tgctccgatc gacgctcgac gacgtgctct ccgggagcga
      961 caggtgagcg ccctcgctcc gttcgcgctc gccttcgtgg ccggcatcgt ctcgttcacg
     1021 tcgccgtgct gcctcccgct gatgcccgga tacgtctcct acgtgagcgg ggtggccggc
     1081 gatggatcga cggcgatgct cgtcagatcg aggacgatgc tctccgccac tctgttcgtt
     1141 tccgggttcg cggccacctt caccctcatg ggtgcggcgg cctccgggct cgggtcgttg
     1201 atcctttacc accggctggt gctcggcagg gtcgcgggtt cgttcgtcat cgcgatgggg
     1261 ctcgccacca tggggctgct ccgcgtgccc ttcctctata aggagcggcg gttcgatctg
     1321 gcgaggatcc ggccgggacc ggttggcgcc gtgccgctcg ggatggcctt cgcgatcggg
     1381 tggaccccct gcatcggtcc ggtgctcgcc gcgatcctga cggccgcggc gaccgttcag
     1441 gacgcgtggc gtggggctgc cctgctgttc gtctattcgc tcggcctcgg cgtccccttc
     1501 ctgctcctgg cgttcgcggt agcgaggacc gggaggctct tcgcctggct ccggcagcac
     1561 ggtcgcgcga tcgaagtcgg gggcggcatc ctggtgctga tgggtgtgct gatgatcacc
     1621 ggccagtggg tccagctgtt cgctcccctg cttcgcctgt tctcccgaac cgggtggccg
     1681 cccgtatgag acggctcgcg ctcctcgtgg tgctcgcggc gctcacactg tgtttgacgc
     1741 catcggtcgc ctgggctcac gcggggttcg tctcctcgag ccccgagccg gggagccagg
     1801 tgagctcggc tccgggggtg gtgacgctcc ggttctccga gccgctcaac accaagctgt
     1861 ctcgggccgc cgtgaccacc cccgatggtc gaagcgtcga gggaacggtc acgagttcgg
     1921 agcagatcgc gatcccgctc tcgacgaacg cgcagggcgt ctatacggtc gactggacga
     1981 ccgtgagcct ggtggacggg cacacgctca ccggctcgtt ccggttcggc gtcggggtct
     2041 ccccgggagc gggagccgag ggtgggacga gcgacgagcc gaacggttcg ggctacctga
     2101 tcgcgatcgg tcggctcgtc gaggacacga gcctgctgct actgctcgga ctgctgctgc
     2161 tcggccggct ggcgcgacgg gagccccgcc tcgcgtgggt gcgaaccccg gtccagcctg
     2221 tgttcctagc ggccttcgcg ggcggcttgg ccgtggtgct cggtgaggcg ttggtagccg
     2281 cgccatctcc gtcgggaagc gcgatcctca cctacctcac gtccggactc cccggatggt
     2341 cgcgactcgg gcgcgtcgtc ctcgaaggcc tcggggtgct cacggcgtgg cgttggccgc
     2401 gcgcacggac cccgcttgcc gtggcggccg tcgttgcgct ggcggcggcc ggacacgccg
     2461 ctgcgatcca gccgcgggcc tggggcgtaa cggtcgaggc cgtgcaccta ctgtcggccg
     2521 gcgtctgggc cggcgggatc ctcgcgctca cgctgcagcg accccccggc ggcctgctcg
     2581 gcatcgacgg ccgggtgctc ctcgaccggt tcacgccggc ggccctgacc gcgttcgcgg
     2641 tcaccgtagc cacggggttg atccgtggag tgcaggaggt cgggagctgg ggcgacctct
     2701 ttggttcctc ctatggggtc gtcctgctcg tcaaggtgct tctcgtcgtg ctgatggttc
     2761 agttctcggt gctggcgtgg cggcggatcg cggtcttccc aaggggagag acggcagccg
     2821 cgatcctcgt gatcggagcc gcggcgctgc tgtccgcgta cccactgcca cctgcccggc
     2881 aggtcgaggc cgcggaggag gcggagccta ccgcccaggc ctcccccatc cccgccggcg
     2941 cggcgctgac gctcggctcg cacgccggcc ccgtgctcgt cgggctcacc gtggagccgg
     3001 cgacgcctgg gaggaatgag ctgacgatct acgtccagag cttggacggc cccgatgcaa
     3061 cggcctccct tccggtccac gcgtccgtgg atggcgacga cgtggcgctc acgcagtgcg
     3121 cggatacgtg ccggaggggc acggcaaacg tcgagggggg tgaggtcgtc gttgtcgacg
     3181 tcggcacagc cagcggtgga caggcgacct tctacctccc cgacctccct gttgcccccg
     3241 gagatcgggt cctgcagcgg atgctcggga cgatgggatc gctcaccagc taccggctcg
     3301 acgagagcct cacctcaggt ctcgagacga ccgtccgaac gacgtacgcg ttcgccgcgc
     3361 cgaactcgtt cgagagccac gtggtcgaga gcgggtcaac gttctcgacg gtgtggatcg
     3421 gcgacacgcg gtacacgcgc gagggcaacg gagtatggaa ggtcgagcga ggcgccccgg
     3481 ctccaccggt ccctacctac atctgggatt cgttccagcc gtaccgggac gcgcgcatcc
     3541 tcggtagcgc tcaggtcaat ggtgtccgaa ccacggagct cgcgttcgcc ggcggcgacc
     3601 aggatctccc cgtgtggttc cgactctggg tcgacgccca ggggtatgtc cacagggcgg
     3661 agatgcgggc cccgggccac ttcatggacc atcggtacta cgactacgac gtaccgatca
     3721 cgatccaacc gccgaaagga gcgagcggat gagacatcgg atgctggtcg ctgcgggact
     3781 gacgctcggc ctcgtggccg cggcgtgctc tcgtcagccg acgagctcgg gacagggccc
     3841 gatcgcggtc gggcgcaccg cgcccgcgtt ctcactgaag tcggcgagcg gcggcacggt
     3901 ctcgctgtcg gagttcgcgg gcaagcccgt gttgctctac ttcagcatgg ggcccgggtg
     3961 aggcggctgc atgcagcagg tggtcgacct gcaacgtgac aaggccttcg aggacctcgg
     4021 cgtccggctg ctcgcgatct cgcccgatcc ggtgagcgcc tggcagcagg agggcggatc
     4081 gttgggcatc accgttccca cgctctccga tccggggaac acggtgtgga cgaagtacgg
     4141 gacgccgggc tggttgatgg ccacgaacga gccgggtcac acgttcttcc tggtgggccc
     4201 cgacgggaag atcgcgtggc tccgcgacta cggcgcgccc gaacacggtg gtgcgatgta
     4261 cgtggagccg gacgagatcg tctcgcaggt gcagcaggcg ctgagcgggt cgtgagacgc
     4321 gctctcgggc tcctcgccgc ggcgagcgtc ttcggcgcgc tcgcccatgc gctcgacatc
     4381 gcgttcggca tgacctggga gatcctctgg gccctcgtcc tcgggttcgt gctgtccgcc
     4441 gcggtccagg cggtcgtgtc gaagtcggag atgacgaggc tccttcccga cgactcgccg
     4501 cggaccatcg cgatcgcctg cggcctgggc gccgcgtcct cctcatgctc ctatgccgcc
     4561 gtggcgctgg cgcgttcgat cttccgcaaa ggcgccaact tcacggctgc gatggcgttc
     4621 gagttcgcct cgaccaatct cgtgatcgag ctcggcatca tcctgtggct gctgatgggg
     4681 tggcaattca cgctggcgga gttcgtcggc ggcccgatca tgatcgcgtt cctcacggtc
     4741 gtgttccgct tcacgctgac acatgagcgg gtggaagagg cccgcgtcca ggccgaccgc
     4801 ggcctggtcg gctcgatgga gggacatgcc gcaatggaca tgtcggtcag cggaggctcg
     4861 atcttcgagc gcttgacctc cccgcgcggg ttcacggcga tcagccacta ctacgtgatg
     4921 gactgggcct cgatctggaa ggacgtcgcg ggagggctgc tgatcgccgg tgcgctcgcg
     4981 gcatgggtgc ccgagagctt ctggcaatcg ttcttcctgg tggatcaccc gctgctggcg
     5041 aagatctggg ggccgctcgt gggtccgatc gtcgcgatgc tgtcgttcgt gtgctcgatc
     5101 ggcaacgtgc cgctcgcggc ggtgctgtgg aacggcggaa tcagcttcgg gggtgtcgtg
     5161 tccttcatct tcgcggacct catcgtgctg ccgatcctga acatctaccg gaagtactac
     5221 ggccggcgcg tgagcctgta cctcctggtg acgttctacg cggcgatggt ggtcgccgga
     5281 ctggtcgtgg agacactctt cgggatcctc ggtctcgtgc ccgaggagcg taacgcgaag
     5341 gtcgtcgagg cgagcgtcac ctggaactac acgacgatcc tcaacatcgc gttcctcaca
     5401 atgacgttgg tgctggtcgt gcgcttcctg cgaacgggag gaccggccat gcttcggatg
     5461 atgaaccggc cagcagaaga gctcgtggcg cccgccccgg aaggagggag acacatgtcc
     5521 gagcacgacg aagacgcaac cgagtacacc tgtcccatgc atcccgaggt cagcagcccc
     5581 accccggggc gctgtcccaa gtgcgggatg gacctcgttc cgagacgcga agacaacgac
     5641 cactgatacc tacggccaag gactcgaggt ggctgagtgg tgaacgtgct ccggattcgg
     5701 gctcaccgcg tggcggcccg agggctcgcc cagcatcgag cgggattccg ctcggtgcct
     5761 gcgggacatc atcacaagac agggtcgctg tcggcagtgt gagcgggtga aagggaggtc
     5821 gaaacgagcg tgatcggaca catgagcggg tggatgtggg acggcggttg gatgtggctc
     5881 tggatggtgt tcatggtgct ggtctgggct gccctgatcg tcttcggcgt gtgggcggta
     5941 cgacgattca ccgaccgctc atcgggaagc gcgcgtcgca tcctggagga gcgattcgcg
     6001 aggggcgaga tcgacgccga tgagttccag cgccaacgcg atctgctcgg gcgatgagat
     6061 cttcctaggc gtcagaagaa gatcccgttc cgctccttcc ccacgatggc gaagggcgtg
     6121 gccgtcatgc cggtggtggc atacgtggcg ccccccgatt cgatcgtcca ccccatggtc
     6181 gtcccggccg tcacgaactc caacgtgaag tcgttcgtcg tgtccgagcc tgacccctcg
     6241 ccggtgacgc cgctccattc cacgtcgaac gacgcggtgg ccgggacctc ctccggtccc
     6301 ccgccgaaca gcgcgttctc gatggtgaag tagtcgtcga tctcgagatc ggtggcgttc
     6361 atcgaggccg tgcccgacaa tccaccaacc cggacgctgc cgaatgggag cgccaccgtc
     6421 cagaacagac cgctcggcgc gatgcccggg ttgaagtcat ggatctgatc cgtgccaact
     6481 gacacgggaa acaggtcgat tcagacgaag ccgaacgcgc cttgacggcg ccgaccatcg
     6541 gtcccgacga agaggccctt catgaagcgc atgtccgctt cccacacgcc ggaggacacc
     6601 cctccggtcg gtgtcccggt cgcgctccat gttccctgcg tcaccgatac gcccacggac
     6661 ccgttgaagt ccgtgatcgt agagggctcg ctt
//
LOCUS       DATBRK010000212        11643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_318963, whole genome shotgun sequence.
ACCESSION   DATBRK010000212 DATBRK010000000
VERSION     DATBRK010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318963"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1161
                     /locus_tag="VIH70_06410"
     CDS             202..1161
                     /locus_tag="VIH70_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8116028.1"
                     /translation="MGVSWNNYNEERWAKWDEPAIQEALQACGAEYISSDAGSSAEQQ
                     LADVENLISQGANALIILAQDGEAILPAVQSALDQGIPVVAYDRLIENPGSLYVTFDN
                     VEIGRLEAEAVFAEVPKGNYVFIKGNSTDANADFLRDGQQEVLQDALDSGAIKNVGES
                     YTDNWDPAVAQTNMEQFLTDNNNNVDAVLSENDGMAGGVVAALEAQGLAGEVAVSGQD
                     GDAAGLNRVALGTQTVDVWKDARELGKAAGDAACAMAQGTALQDVANTEAFDSPGGNS
                     MTSIFLTPQAITQDNLDVVLDAGWISQEDLCQGVDPGTVDACG"
     gene            1251..2564
                     /locus_tag="VIH70_06415"
     CDS             1251..2564
                     /locus_tag="VIH70_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8116029.1"
                     /translation="MAETKVSAAEREPEAQAAPEERGKRPLRELFRSAEVDTRLLGML
                     GALAIIWILFQIITPNHTFLTPRNLWNLSVQTAVVAIMATGMVLIIVTRNIDLSVGAV
                     MAAVGMAMAIVQHDWLPEWIGLDHPLTWVIAVAVGLVLGAAIGAFQGTIVAYVGVPSF
                     IVTLGGFLVWRGVAWWMASGQTIAPMDTLFRKLGGGSTGTVGGTWSWILGIAACVGIV
                     MLQFSKRRQRNRYGFRQRPMWAEVTIAVIACGAVLLLVGFLNRYYLPPALAAQAGKTG
                     QNISLGLAYPVLIAIGVGIVMTFIATRRRFGRSVYAIGGNPEAAELAGIKTRRTIVRT
                     FTLMGLLVGLAAAVQIARLNAAVSSLGTGMELNVIAAAVIGGTSFAGGVGTIVGALLG
                     ALIMQSLVSGMVLLGIEAPLQDIVVGIVLVLAVTLDTVVRRRSAK"
     gene            2564..3346
                     /locus_tag="VIH70_06420"
     CDS             2564..3346
                     /locus_tag="VIH70_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8116030.1"
                     /translation="MDRSGTPLVELRDIRVAFGGVHAVDGVTLDLYPGEVVGLVGGNG
                     AGKTTLIRTLSGAHPADSGQIRVEGQPVTISHPRDAKALGVETIYQNLALADNIDAPA
                     NVFLGRELTTRFGNLDDSAMEDATRKLMGRLNPKFQNFVVPVASLSGGQRQSVAIARA
                     VHFNARILIMDEPTAALGPAETKQVGELIRQLREEGLGIFLISHDIHDVYDLSDRISV
                     MLQGKLVGTVNKDDVTTDEVLAMIILGKLPGEVTQKDLAELH"
     gene            complement(3361..5034)
                     /locus_tag="VIH70_06425"
     CDS             complement(3361..5034)
                     /locus_tag="VIH70_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116031.1"
                     /translation="MTQLSARSVTDVPAAPGATAPEPPARPAGRTVRIFGRTYPFVAP
                     SIRDPRLHLAAVIISIHVLGQIALGFRVSVPQILVAILVCALIEVGWTLYTTNTLVWP
                     ASAMLTGSGVALILRLSDMQSDDHWSWRGWYVFALVAGLSLLTKYVIRYRGTHIFNPS
                     NVGLVVAFLLLGSTRIEPLDFWWAPFDGWMMVAYLIILVGGLLITARLHLLGMSAAFW
                     LTLAVGIGILAASGHCMTARWSFEPVCGTHFWWVIVLSPEILIFLFFMITDPKTTPAG
                     RVARIAFGVAVAMVCTLLIAPQTTEFGAKVALLAGLVVMCVARLFTEKLLPAPRSERD
                     RLGAYLVGPGGRGESYLAPGRALARGAIAGAAVVLLGTAVVAAGAPAREAFVAASGSV
                     TTSRPAEPQLDVHVDPSTLPQVTVDPEVVDRGDLTRADAQAIALTLAENLEVEAQALR
                     RGEKSLLSSVDDGQRLTTLQRRVDDVIETGRADVPHYTFDELHVVLVQPQGQAGLSLG
                     LQAGGSVEAVMYGVDEKEASRTSSPFSRTFVLSRPTGDRWLLVEVGPNP"
     gene            5141..6202
                     /locus_tag="VIH70_06430"
     CDS             5141..6202
                     /locus_tag="VIH70_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY8116032.1"
                     /translation="MIPTEPIGSIPRPKTLIDGMAAFAEGTISEEELGVLQADALTDT
                     IARFEETGSPVISDGEQTKSSFVTYPLEGVTNLAADGVVIPFADGHTRQLPRLASAPF
                     RFGTYAASYLEEAKHRAHVPVKQAVIAASALSLLYPQDGIEGYPRETFLDDLVGECET
                     DIRRSLEAGAHKVQIDFTEGRLACKLDPSGGLLRDFVALNNRVLERFSDDERTRIGVH
                     TCPGGDMDSTHSADVDYAELLPDLFQLNVGNVYVELAAEPDPGRVLYIIATHLRPEQR
                     IFVGVTDPIDPAVESPDQVRDRVLEAADRLPKDRLGTTDDCGFAPFADDGSTSRDVAF
                     DKIRARIEGTRLAEERLGL"
     gene            complement(6199..7920)
                     /locus_tag="VIH70_06435"
     CDS             complement(6199..7920)
                     /locus_tag="VIH70_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY8116033.1"
                     /translation="MAPPRADLVELRVLDGPNLYFPRPAVKLTIAVGGWLEVPEERLA
                     AALERVGATGQPGRAGSDQRRRTVARLAARLTWRLATASGVRLAVRSRPGPERDQVVV
                     AFPWRRRGAAEALGREVAPLLHEVGGRRSVERLLIEATERVAAAKPGTEPEVRDPGIP
                     VVSVTGTNGKTTTVRLLAHIVRSARRTVAYSSTDGVYRGDGELIEDGDYSGYGGAARA
                     LAEDPDVAVLETARGGILLRGIGVMHNDVAVVTNVSEDHLGLHGIHTLDQLAEVKATI
                     TRITRPDGWDVLNADDPRVLSMRRGSTGRTWLCSHDPWHPAIRDALAGGGRATVPLDG
                     WVTVLDGSATRPLVELVDVPVTIAGVSRHNVMNAMQAASAALAIGLPERAVVKGLKTF
                     VTDPERNPGRANLFELGGRVVVVDYAHNEAGMRGFTEMCNCLRKPGREIWLVICAAGD
                     RTDRILHGFGYAAARGADHVAVAELLRYLRGRDRLDVIERLMAGVRDGGADEVDAYAD
                     ELAGLRAVVGRSERGDVIAVTALGMRAEVFAWLDEAGARRLTPARVKQLVRRARDGKD
                     APAKSRR"
     gene            complement(7935..9266)
                     /locus_tag="VIH70_06440"
     CDS             complement(7935..9266)
                     /locus_tag="VIH70_06440"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8116034.1"
                     /translation="MAGDDSQIPDGDEQTGDEVTPSNVGAERRRSLVRRIAVDVTPLR
                     SSRDFRRLWLGLLASTFGSQFTIVATYVQVYRLTESTAAVGLLGLVGFLGLVAGTLGG
                     STFLDAFDRRKILIASQLGYLASSGLLFAGALHGDPPLALIYVAVAGVAATSAIDSPT
                     RSAMTPRLLDRGLLPTAAALNQIVWNGAGLIGPALAGVVVGTLGFSWAYGIDLISIGL
                     LLLASISIRPMPPADGERAAVGWRAVREGFAFLRGRQVLQSTFVIDIIAMVFGMPRAL
                     FPVLILTQFDRSDTVVGLLFAAPAVGAFIGAATTGWVGRVRHQGLAVIWAVVVWGAGI
                     AAFGLVGAHLWLGLFFLAVAGAADVISAIFRNTILQVTVPENLRGRLFGIHILVVTGG
                     PRLGDLEAGLVAAAFSPTASVVSGGLLCIAGAGLVAWRVPAFRRYHAGDPG"
     gene            complement(9551..10882)
                     /gene="gabT"
                     /locus_tag="VIH70_06445"
     CDS             complement(9551..10882)
                     /gene="gabT"
                     /locus_tag="VIH70_06445"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEY8116035.1"
                     /translation="MDIPQERKVVTEIPGPRSREWFERRAAAVPQGVFNTHPIVTARA
                     SGSIIEDVDGNRLIDFATGIAVLNVGHTSPEVVAAAQHQLELDTHTCFHVTANEPYIE
                     LAERLNALAPGDAPKKTMFANSGAEAVENAVKIARKATGRQAIVTFDHAFHGRTLLAM
                     SLTAKVMPYKQGMGPFAPEIYRLPFAYPYRWPSRPERCAEESLDYARDEIHKHIGQEN
                     VAAVILEPIQGEGGFIVPAPGFVKGLAEFCAEHGILFIADEIQSGMGRAGRWFAIEDE
                     GVVPDLITSAKSLGGGLPISAVTGRADVMDSVHVGGLGGTYGGNPVAAAAALAVLDQI
                     EKEGLLERSRTLGEKVLGRLGEMQQRHQVIGDVRGRGLMTAIELVSNRETKEPLPGAD
                     GNAIVQRCLGEGLVILKVGTYDNVIRLLPPLTIDESLLDEGLQILDDAIGA"
     gene            complement(10951..11388)
                     /locus_tag="VIH70_06450"
     CDS             complement(10951..11388)
                     /locus_tag="VIH70_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116036.1"
                     /translation="MPMRRYLVVANQTLAGEPLVSRLRELSHAGPCAFFLIVPATPPR
                     DHPWTEGEARATAGARLDEALMRLSGLEVKVEGEVGDGNPMLAIDDAIRDRGPFDEII
                     LSTLPPGLSKWLRLDLPHRVSASFPIPMTHVVGEPAHVGASRR"
BASE COUNT         1832 a         4034 c         4107 g         1670 t
ORIGIN      
        1 gggccatccg gttcgtagaa cgggagggtc ggtgcgtcac cgggacgttc cgtccagaag
       61 ggggggagtg cattgaagcg tcggttgttg gcggtcctcg ccctgtcggt tctgctgctc
      121 gccgcgtgtt cgaacgacga cgacggcggt ggcggatcga cgggagcgac tggaggagcc
      181 accggcggcg gcaacgtcac cgtgggggtt tcctggaaca actacaacga ggaacggtgg
      241 gccaagtggg acgagcccgc gattcaggag gccctgcagg cctgcggcgc ggagtacatc
      301 tccagcgacg ccgggtcgtc ggccgagcag cagctggccg acgtcgagaa cctaatctcg
      361 cagggcgcga acgcgctcat catcctggcg caggacggtg aggcgatcct gccggccgtg
      421 cagagcgcgc tcgaccaggg catcccggtc gtcgcgtacg accggctgat cgagaacccg
      481 ggctcgctgt acgtcacgtt cgacaacgtc gagatcgggc gtctggaagc ggaggccgtc
      541 ttcgcggagg tccccaaggg gaactacgtg ttcatcaagg ggaattcgac ggacgcgaac
      601 gccgacttcc ttcgggacgg gcagcaggag gtcctccagg acgcgctgga ctcgggtgcc
      661 atcaagaacg tcggcgagtc ctacaccgac aactgggacc cggcggtcgc gcagacgaac
      721 atggagcagt tcctgacgga caacaacaac aacgtcgatg ccgtcctctc ggagaacgac
      781 ggcatggccg gcggcgtcgt ggccgcgctc gaggcccagg gtctggcggg ggaggtcgcg
      841 gtgtccgggc aggacggcga cgccgccgga ctgaaccgcg tggcgctggg gacgcagacc
      901 gtggacgtgt ggaaggacgc ccgggagctc ggcaaggccg cgggtgatgc agcttgcgcg
      961 atggcgcagg gcacggcgct ccaggacgtc gcgaacacgg aggcgttcga ctcgccggga
     1021 gggaactcga tgacctcgat cttcctgacc ccacaggcga tcacgcagga caacctcgac
     1081 gtcgtgctcg acgccgggtg gatctcccag gaagacctgt gccagggtgt cgatccgggc
     1141 acggtggatg cgtgcggtta gcgcacgagg cctgacacgc gttgacccgg gggcgctcgc
     1201 ctcacccgtc ggggtgaggt gagcgcccca cttgacatag gattcgaccg gtggctgaga
     1261 ccaaggtttc cgcggccgaa cgagagccgg aggcgcaggc ggcgcccgag gagcgaggca
     1321 aacgcccgct ccgcgagctc ttccggtcgg ccgaggtgga cacccggctc ctggggatgt
     1381 tgggagcctt ggcgatcatc tggatcctct tccagatcat cacgccgaac catacgttcc
     1441 tcacgccgag gaatctgtgg aacctgtcgg tgcagaccgc cgtcgtcgcg atcatggcga
     1501 cggggatggt cctgatcatc gtgacgcgca acatcgacct gtccgtcggc gccgtgatgg
     1561 ctgccgtcgg catggcgatg gcgatcgtcc agcacgactg gctgccggag tggatcgggt
     1621 tggatcaccc gctgacgtgg gtgatcgccg tggcggtcgg gctggtcctc ggggcggcga
     1681 tcggcgcgtt ccaggggacc atcgtcgcgt acgtcggcgt gccatcgttc atcgtgacgc
     1741 tcggggggtt tcttgtgtgg cggggcgtcg cctggtggat ggcgagcggc cagacgatcg
     1801 cgcccatgga cacgctcttc cgcaagctgg ggggcggctc cacgggaacg gtgggcggga
     1861 cgtggagctg gatcctcggc atcgccgcgt gtgtggggat cgtcatgctg cagttcagca
     1921 agcggcgaca gcgcaatcgg tacgggttcc ggcagcgtcc gatgtgggcc gaggtcacga
     1981 tcgcggtgat cgcctgcggg gccgtgctcc tgctcgttgg gttcctgaac cgctactacc
     2041 tgccgccggc gctcgcggcg caggccggca agaccgggca gaacatctcg ctcggccttg
     2101 cctacccggt gctcatcgcg atcggcgtcg gcatcgtgat gacgttcatc gcgacgcggc
     2161 ggcgcttcgg ccgctccgtt tacgcgatcg gggggaaccc cgaggcggcc gagctcgcgg
     2221 gcatcaagac caggcggacg atcgtcagga cgttcacgct gatgggcctg ctggtcggtc
     2281 tcgcagccgc ggtccagatc gcccggctca acgcggcggt gagcagcctc gggaccggga
     2341 tggagctcaa cgtgatcgcg gctgcggtga tcggagggac atcgttcgcc ggcggcgtcg
     2401 gcacgatcgt cggggcgctc ctcggcgcgc tcatcatgca gtcgctcgtg tcgggcatgg
     2461 tgctgttggg catcgaggcg ccgctccagg acatcgtcgt cgggatcgtg ttggtcctgg
     2521 ccgtgaccct ggacacggtc gtccgccgga ggtcggcgaa gtgatcgacc ggtcggggac
     2581 cccgctcgtc gaactgcgcg acatccgggt cgcgttcggc ggcgtccacg cggtggacgg
     2641 cgtaaccctc gacctgtatc cgggcgaggt cgttggcctt gtcggcggca acggggccgg
     2701 caagaccacg ctgatccgaa cgctctcggg cgcccacccc gccgactcgg gccagatccg
     2761 tgtcgagggc caaccggtca cgatctcgca cccccgcgat gcgaaggcgc tcggggtgga
     2821 gacgatctat cagaacctgg cgctcgcgga caacatcgac gcacctgcca acgtgttcct
     2881 gggacgcgag ctcacgactc ggttcggcaa cttggacgac agcgcgatgg aggacgccac
     2941 gcggaagctg atgggcaggc tgaacccgaa gttccagaac ttcgtcgtgc ctgtcgcctc
     3001 gctctccggt ggacaacgcc agtcggtggc gatcgcccga gcggtccact tcaacgcccg
     3061 catcctgatc atggacgagc ccaccgccgc gctcggcccc gccgagacca agcaggtcgg
     3121 cgagctgatc aggcagctgc gggaggaggg cctcgggatc ttcctgatca gccacgacat
     3181 ccacgacgtg tacgacctgt ccgaccggat cagcgtgatg ctccagggca agctcgtcgg
     3241 cacggtcaac aaggacgacg tgacgacgga cgaggtcctg gccatgatca tcctgggcaa
     3301 gttgccgggg gaggtcacgc agaaggacct ggccgaactg cactgacccc gcccgctccg
     3361 tcaggggttg ggcccgacct ccacgagaag ccatcgatcg ccggtcggcc ggctgagcac
     3421 gaaggtgcgc gagaacggcg aggacgtccg gctggcctcc ttctcgtcca cgccgtacat
     3481 gaccgcttcg accgacccgc cggcctgaag accgaggctg aggccggcct gaccctgggg
     3541 ttgcacgagg acgacgtgga gctcgtcgaa cgtgtagtgg gggacgtcgg cgcgtccggt
     3601 ctcgatcacg tcgtcgacgc gtcgctggag cgtggtcagg cgctggccgt cgtcgaccga
     3661 ggagaggagg ctcttctcgc cgcggcggag cgcctgagcc tcgacctcca ggttctcggc
     3721 gagcgtgagg gcgatcgcct gcgcgtccgc acgcgtcagg tcgcctcgat cgaccacctc
     3781 ggggtcgacg gtgacctgcg gaagggtgga gggatcgacg tggacgtcga gctgaggctc
     3841 ggcgggccgg cttgtcgtca ccgagccgct ggcggcgacg aacgcctccc gcgccggcgc
     3901 ccccgccgcc acgaccgcgg tccccagcag cacgaccgcg gcaccggcga tcgccccgcg
     3961 cgcgagcgcc cggccgggcg cgagatacga ctcgccgcgc ccgcccggcc cgacgaggta
     4021 ggcgcccagc cggtcgcgtt cggatctcgg ggccggcagc agcttctcgg tgaacagccg
     4081 cgccacgcac atgacgacga ggcccgccag cagcgcgacc ttggcgccga actcggtcgt
     4141 ctgcggcgcg atcagcagcg tgcagaccat cgcgacggcg acgccgaacg cgatccgtgc
     4201 gacccgtccc gccggcgtcg tcttcgggtc cgtgatcatg aagaacagga agatgaggat
     4261 ctcgggggag aggacgatca cccaccagaa gtgggtcccg catacgggct cgaacgacca
     4321 ccgcgcggtc atgcagtgcc ccgaggccgc gaggatgccg atgcccaccg cgagcgtgag
     4381 ccagaacgcc gccgacatcc ccagcaggtg cagcctggcg gtgatcagca ggccgcccac
     4441 gaggatgatc aggtaggcca ccatcatcca accgtcgaac ggcgcccacc agaagtcgag
     4501 cggctcgatc cgcgtgctgc cgaggagcag gaacgcgacg acgagcccga cgttcgacgg
     4561 gttgaagatg tgcgtgcccc ggtaacggat cacgtacttc gtgagcagcg acagcccggc
     4621 cacgagcgcg aacacgtacc acccgcgcca gctccagtgg tcgtcgctct gcatgtcgct
     4681 cagacgcagg atcagcgcca cgccgcttcc ggtcagcatc gcgctggccg gccagaccag
     4741 ggtgttcgtt gtgtagaggg tccatcccac ctcgatgagg gcgcacacga ggatcgccac
     4801 gaggatctgc ggaaccgaca cgcggaaccc gagcgcgatc tgacccagga cgtggatcga
     4861 gatgatcacg gccgcgaggt ggagccgcgg gtcgcggatg ctcggcgcga cgaacgggta
     4921 ggtgcgcccg aagatccgca ccgtgcgtcc ggccggcctt gcgggcggct ctggcgccgt
     4981 ggcaccgggc gccgccggca cgtccgtcac cgagcgtgcc gagagctgcg tcatcggtgg
     5041 tggcggctcc ttcaggacgt ccctttccag cgcgggtccg accggagcgg ttatcgtacg
     5101 cgctcgcccg atcccgtttg acccgagcaa ggagaccccg atgatcccga ccgagcccat
     5161 cggcagtatc cccagaccca agaccctcat cgacggcatg gccgcgttcg cggaggggac
     5221 gatctccgag gaggagctcg gcgtgctgca ggccgacgcg ctcacggaca cgatcgcccg
     5281 cttcgaggag acgggctcgc cggtcatctc cgacggcgag cagaccaagt ccagcttcgt
     5341 gacgtatccg ctggagggcg tgacgaacct cgcggcggac ggcgtcgtga tcccgttcgc
     5401 ggacgggcac acgcggcagt tgcccaggct cgcgtcggcg cccttccggt tcggtacgta
     5461 cgcggcgagc tacctggagg aggcaaagca ccgcgcgcac gttccggtga agcaggccgt
     5521 catcgccgcc tcggccctca gcctgctcta cccgcaggat gggatcgagg gctacccgcg
     5581 tgagacgttc ctggacgacc tggtcggcga gtgcgagacg gacatcaggc gatcgctcga
     5641 ggccggcgcg cacaaggtgc agatcgactt caccgagggc cgtctcgcgt gcaagctcga
     5701 tccctcgggc gggctgcttc gagacttcgt cgcgctcaac aatcgggtgc tcgagcggtt
     5761 ctcggacgac gagcgcaccc ggatcggcgt gcacacgtgc cccggcggcg acatggactc
     5821 gacacacagc gccgacgtcg actacgcgga gctgctgccc gacctgttcc agttgaacgt
     5881 cggaaacgtg tacgtcgagt tggcggccga gccggacccg ggccgggtcc tgtacatcat
     5941 cgcgacgcat ctgcgccccg agcagcggat cttcgtcggc gtgaccgacc cgatcgaccc
     6001 ggccgtcgag tcccccgacc aggtccgcga ccgcgtgctc gaggcggcag accgcctgcc
     6061 gaaggaccgg ctcggcacga cggacgactg cggcttcgcg cccttcgcgg acgacggctc
     6121 gacctcgcgg gacgtggcgt tcgacaagat ccgcgctcgg atcgagggca cgcggctcgc
     6181 cgaggagcgc ctcggcctct agcgccggct cttggccggg gcatccttgc cgtcgcgtgc
     6241 gcggcggacc aactgcttga cccgagcggg cgtgagccgc ctcgctccgg cctcgtccag
     6301 ccacgcgaac acctccgccc gcatcccgag cgcggtcacg gcgatcacgt cgccgcgttc
     6361 cgaacgtccc acgaccgcgc gcagccccgc gagctcgtcg gcgtaggcgt ccacctcgtc
     6421 ggcgccgccg tcccggaccc ccgccatgag acgctcgatg acgtcgagac ggtcgcggcc
     6481 gcgaaggtag cgcaggagct ctgcgaccgc gacgtggtcg gcgcctcggg ctgcggcgta
     6541 cccgaagccg tgcaggatcc ggtcggtccg atcgccggcc gcacagatca cgagccagat
     6601 ctcccgaccc ggcttgcgca ggcagttgca catctccgtg aacccccgca tccccgcctc
     6661 gttgtgcgcg taatcgacga cgacgacgcg gccgccgagc tcgaacaggt tcgcccggcc
     6721 cgggttgcgt tcgggatcgg tgacgaaggt cttcagccct ttcacgaccg cgcgttcggg
     6781 cagtccgatc gcgagcgcgg cggaggccgc ctgcatcgcg ttcatcacgt tgtggcgcga
     6841 gacgcctgcg atcgtgaccg gcacgtcgac gagctcgacc agcggtcgcg tcgcggaacc
     6901 gtcgagcact gtgacccaac cgtccagtgg gaccgtcgcg cgtccgccac cggcgagcgc
     6961 atcgcggatc gccgggtgcc acgggtcgtg cgagcacagc cacgtccgtc ccgtcgagcc
     7021 gcgacgcatc gagagcacgc gcgggtcatc cgcgttcagc acgtcccaac cgtccggtcg
     7081 cgtgatgcgc gtgatcgtcg ccttgacctc ggccagctgg tccagggtgt ggatgccgtg
     7141 cagccccagg tggtcctccg acacgttcgt cacgaccgcg acgtcgttgt gcatcacgcc
     7201 gatcccgcgg agcaggatgc cgccgcgcgc ggtctcgaga acagcgacgt caggatcctc
     7261 ggccagcgcc cgggcggcgc cgccgtaccc ggagtagtcg ccatcctcga tcagctcgcc
     7321 gtcgcctcgg tacaccccgt ccgtggacga gtacgcgacg gtcctgcgcg ccgaccgcac
     7381 gatgtgcgcg agcagccgga ccgtggtggt cttgccgttc gtgcccgtca ccgagaccac
     7441 cgggatgccc gggtcgcgga cctctggctc ggtaccgggc tttgcggccg cgacccgttc
     7501 ggtggcctcg atgaggaggc gctccaccga acggcgaccg cccacctcgt gcagcagcgg
     7561 cgcaacctcg cgaccgagcg cctcggcggc gccgcgccga cgccagggga acgccacgac
     7621 gacctggtcg cgttcgggac cggggcggct tcggaccgcg agccgcaccc cgctcgccgt
     7681 cgcgagccgc cacgtgagtc gggctgccag gcgggcgacc gtccgtcgcc gctggtccga
     7741 tcccgcgcga cccggctgtc cggtcgcgcc cacgcgttcg agcgccgcgg ccaggcgttc
     7801 ctccgggacc tcgagccagc ccccaacggc gatcgtgagc ttgaccgcgg ggcgcgggaa
     7861 gtacaggttc ggaccgtcga gcacccggag ctcgacgagg tcggcgcgcg gtggggccac
     7921 gggcgcgagc ttagtcagcc ggggtcgccg gcgtggtacc ggcggaacgc ggggacgcgc
     7981 cacgcgacga ggccggcacc ggcgatacac agcagccctc cggagacgac cgaggccgtg
     8041 ggcgagaacg cggccgcgac cagacccgcc tccaggtcgc ccagccgagg gccgccggtc
     8101 accaccagga tgtggatgcc gaacaaccgt ccgcgcaggt tctctgggac ggtgacctgc
     8161 aggatcgtgt tgcggaagat ggccgagatc acgtcggccg cgcccgccac cgccaagaag
     8221 aacagcccga gccacaagtg ggcgccgacg aggccgaagg ccgcgatgcc cgcgccccac
     8281 accacgacgg cccagatgac ggcgagtccc tggtgccgga cccgccccac ccatcccgtc
     8341 gtcgcggcgc cgatgaaggc gccgaccgcc ggcgcggcaa acagtaggcc gacgacggta
     8401 tcgctccgat cgaactgcgt gaggatcaac accgggaaca gcgcccgggg catcccgaat
     8461 accatcgcga tgatgtcgat cacgaacgtc gactggagga cctggcgtcc acgcaggaac
     8521 gcgaacccct cgcgaaccgc gcgccatccc accgccgcgc gctcgccgtc ggcggggggc
     8581 atcggccgga tcgagatcga agccaggagc agcagcccga tcgagatcag atcgatcccg
     8641 tacgcccacg agaacccgag cgtccccacc accacgcctg cgagcgccgg accgatcaga
     8701 ccagccccgt tccagacgat ctggttgagg gcggcggccg tgggaagcag ccctcgatcg
     8761 agcagccgag gggtcatcgc gctccgggtc gggctgtcga tcgcactcgt ggccgcgacg
     8821 ccggcgactg ccacgtagat cagcgcgagc ggcggatcgc cgtgcagcgc gccggcaaac
     8881 agcagtccgg aggacgccag gtagccgagc tgggacgcga tcaggatctt ccgccgatcg
     8941 aacgcatcca ggaacgtgct cccgccgagg gtccccgcca cgagcccgag gaagccgacg
     9001 agcccgagga gcccgacggc ggcggtcgat tcggtcagcc gatagacctg gacgtaggtc
     9061 gcgacgatcg tgaactgcga gccgaaggtc gacgcgagca acccgagcca caaccgccgg
     9121 aagtcgcgcg acgagcgcag cggcgtgacg tcgaccgcga tgcgccgcac gagcgagcgc
     9181 cggcgctcgg cgccgacatt ggagggggtg acctcgtcgc ccgtctgctc gtctccgtcg
     9241 ggtatctggc tgtcgtcgcc cgccacatcg ccgacggtag cagtcgaccg tctctcgacg
     9301 atggaaccca gggaggcgaa cggcggtcag gtcgatggag ccgaacagga ggagaccgat
     9361 gcgtcgatgg ctcgttgtga tgctcgcggc cgcttcgtgg atcgggcttg ccgcgacccc
     9421 cgccctcgcc ggcggcgcac ccacgccgcc caaacccgcg ttctacgtgg cgttgggggt
     9481 cttcgccctg gtcgtgtgcc ttgcgcttgt cgtcgccgta ggctcggcga tcgtcgctgc
     9541 tcggcgcctg ctacgcgccg atcgcgtcgt cgaggatctg gagcccctcg tccagcaagc
     9601 tctcatcgat cgtgagcggg ggcaggagcc ggatgacgtt gtcgtaggtc ccgaccttca
     9661 ggatcacgag tccctcaccc aggcaccgct gcacgatcgc gttgccatcg gcgccgggta
     9721 gaggttcctt cgtctcgcga ttgctcacga gctcgatcgc cgtcatcagc ccacggcccc
     9781 gaacgtcgcc gatcacctga tgacgctgct gcatctcgcc cagccggccg agcaccttct
     9841 cgccgagcgt gcgcgagcgc tcgagcaggc cttccttctc gatctgatcg agcacggcga
     9901 gcgcggccgc ggcggccacg ggattccctc cgtacgtgcc gccgagcccc ccgacgtgga
     9961 cggaatccat gacgtcggct cggcccgtga cggcgctgat cggcaggccg ccgccgagcg
    10021 acttggcgct cgtgatcagg tccggaacga caccctcgtc ctcgatcgcg aaccagcgcc
    10081 cggcgcggcc catgccgctc tggatctcgt cggcgatgaa caggatcccg tgctcggcgc
    10141 agaactccgc gagacccttc acgaaccccg gcgccgggac gatgaagccg ccctcgccct
    10201 ggatcggctc gaggatcacc gcggcgacgt tctcctggcc gatgtgcttg tggatctcat
    10261 cgcgcgcgta gtcgagcgac tcctcggcgc agcgctcggg cctgctcggc cagcggtacg
    10321 ggtaggcgaa cggcagccgg tagatctcgg gcgcgaacgg gcccatgccc tgcttgtacg
    10381 gcatcacctt ggccgtgagc gacatcgcga gcagggttcg gccgtggaac gcgtggtcga
    10441 acgtcacgat cgcctgtcgg cccgtcgcct tccgcgcgat cttgaccgcg ttctcgacgg
    10501 cctcggcgcc cgagttggcg aacatcgtct tcttcggcgc gtcgccgggc gcgagggcgt
    10561 tcagccgttc ggccagctcg atgtagggct cgttggcggt cacgtggaag caggtgtgcg
    10621 tgtcgagctc gagctgatgc tgcgccgccg cgaccacctc gggggaggtg tggcccacgt
    10681 tgagcacggc gatcccggtg gcgaagtcga tcagccggtt gccgtcgacg tcctcgatga
    10741 tcgagcccga ggcccgggcc gtgacgatcg ggtgcgtgtt gaacacgccc tgcggcacgg
    10801 ccgcggcgcg gcgctcgaac cactcgcgcg acctcggccc cgggatctcg gtgacgacct
    10861 tgcgctcctg cgggatgtcc acgtgccgac gcctcctcgt gaggatggcg tcagtttccc
    10921 acgcgaccgc gcggccgagc gaaccggttg ctaccggcgc gaggctccca cgtgcgcggg
    10981 ctcgcccacg acgtgcgtca tggggatcgg gaagctcgcc gagactcggt gcggaagatc
    11041 gagcctcagc cacttcgaca ggcccggcgg cagcgtcgac aggatgatct cgtcgaacgg
    11101 ccctcggtcg cggatcgcgt cgtcgatcgc gagcatcggg ttcccgtcgc ccacctcgcc
    11161 ctcgaccttc acctccagcc ctgacagtcg catcagggcc tcgtccaggc gggctcccgc
    11221 cgtggcgcgg gcctcgccct cggtccacgg gtggtcccgc ggaggcgtgg cagggacgat
    11281 caggaagaac gcgcaggggc cggcatgcga gagctcgcgg agcctcgaga ccaggggttc
    11341 gccggcgagg gtctggttgg cgacgaccag gtaacggcgc atcggcacca cctccaactc
    11401 cccaccttac gaccgaggac ccgaaagggg gctcgcaggc ctcggcgaga ccccgatccg
    11461 cgcttttcgg catactttga tgaggacgac ctccagacca ctcggggtcc cggcgcagga
    11521 aggtgaggtc cggatgggcg acgcgaggag ctacaagatg tacgtcggcg gcgagtgggt
    11581 ggacgccgcc gagggccaga cccgcaagat cgtcagcccc gccaacgggg acgtgatcgc
    11641 cga
//
LOCUS       DATBRK010000213         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_897382, whole genome shotgun sequence.
ACCESSION   DATBRK010000213 DATBRK010000000
VERSION     DATBRK010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897382"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..171
                     /locus_tag="VIH70_06455"
                     /pseudo
     CDS             1..171
                     /locus_tag="VIH70_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515078.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(456..854)
                     /locus_tag="VIH70_06460"
     CDS             complement(456..854)
                     /locus_tag="VIH70_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116037.1"
                     /translation="MGVTTKERVQSVVVGLVVIVFVGSALAYLVAPSRMLSIVGIESD
                     TQDDFLVRTLAAALLALAPGAWAARRRDSSMSQRGVLIGLAVYLFLSSLVDLHAYIRD
                     IVGPASLPSIAVRIVLGGVIVWLIPHPENG"
BASE COUNT          370 a          643 c          612 g          325 t
ORIGIN      
        1 cgcgcctccg accgatgcga gggctgaagc gtgaccgaac tgcgagggtg atcgtcggcg
       61 ggcacgcctt cgtccagaac ctccgccggg gcttctacga cctcgaggac cgatgtccga
      121 cccacggctc gcctcgccga cgcgttcgcg gaactcgctc tcgtgatctg attcctgcag
      181 ggacgggtag gtccaccgcg cccgggctct agccaacgaa acagagccca ttcgtgaacg
      241 acttgcatca aacaacgtcg aggccgtatg gcacgcgccc aaccggtaat cgatgcgcct
      301 caccgtcccg gggtcccttg ttcctaatgg tgcagggccg ggccacagcc cgaatcccga
      361 ccgggctcga cccgctcgcc gggacggagg aaccctcgcg accagcacgc tcgtcgcgat
      421 tcaacggtct aggtgctcag accgctcttc agacttcatc cattctcggg atgtgggatg
      481 agccacacaa tcacgccgcc caggacgatc cgaaccgcga tgcttggcag cgaagcgggc
      541 cccacgatgt cgcggatgta ggcatgcagg tcgaccagcg agcttaagaa caagtagacg
      601 gctagaccta tcaggacgcc tcgctgggac atagagctat ccctccgccg cgcagcccat
      661 gcgccgggag ccagcgcgag aagcgccgcg gccagcgtcc gaacaagaaa atcgtcctga
      721 gtgtcgctct cgatcccgac aatgctcagc atcctcgacg gcgcgaccag gtaggcaagg
      781 gctgatccga cgaagacgat caccacgagg ccgaccacca cagactggac ccgctccttc
      841 gtcgtgactc ccaccgagcc agatgtatca cgagaacgct caccgacacc ggccatgtaa
      901 gaccagcgag gcatctactc cggaggccta acgcggagac ccgcgtgatc aatgtagacg
      961 gaccgtcagg ggttgtggct tcacattctg gcatcgcggc gtcagtctcc cggacgggca
     1021 ccgcccattg atgagcgggt tcgccatgca tgggcgatat ctgctgatgg acaacaagcc
     1081 agcgtccgtc ggcgtgtcgg ccgaacacga tcgtctctat ctcaagcttg aagggcacgg
     1141 aaactcccga tccgacctca agcaaccagt tcgtactcgt ggatgagacc acccagcacg
     1201 tcgcgccttc gagcccgtct ctcctcgggc gcacctcgga gggactcagg ccgctgccgc
     1261 ggcgtcctca ggtcgagacc tcggtgaggc ctggcctcgt tgtagtgagc cgcgtaggtc
     1321 cggagcgtct cctccaggtg acgacgtccg agcaccagca tccatcggct tggtcgctcg
     1381 ccgtctcctc gagcacgtcg cccctcgtgt tcgctgagtg gagcgggatc gacgccggcg
     1441 tcctgacagg actgcgtgaa ctccccatcg acgagttggc gacagcgaca cacggggctg
     1501 cttgaggcgg cgctctctac gcttgtcgag tccggtgagt cagcggccat tgcccggaac
     1561 gaaggtgtcg tgctcacctc gacccgtcac gaggccaagg ccaaagtcgc gcacgtgtcc
     1621 aaccgctttg aaacgggtag ggacgcacaa gcgcggaaga cgagccgaag gaggtccgtc
     1681 atggacaact cgaggagcat gcagccgcgc agtggttgga cctggaaggt atgggtcgga
     1741 atcgttgtcg gcctagcggt cgttgccggg ctcatcgttc tcatcgccgc gaacgccggc
     1801 ggaggcccgg gcggcggtgg ctcgggcggg atctactgac ccaacgtttc agccaaatcg
     1861 tgtcggtcgt gggggtcctt gaacctccgc gggttccgat gcacatgtac gcggtcgtgt
     1921 cgtgggaccc gcaaggatga tcgcgtgatc
//
LOCUS       DATBRK010000214         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2490669, whole genome shotgun sequence.
ACCESSION   DATBRK010000214 DATBRK010000000
VERSION     DATBRK010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490669"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VIH70_06465"
     CDS             complement(<1..231)
                     /locus_tag="VIH70_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY8116038.1"
                     /translation="MIRVGVIGACGRMGLMVCRALADSHDFALVAAIDRSKIGQKIGQ
                     MIGHPEIDAPVSDELDRLLEANAEVAIDFTHPD"
     gene            complement(228..1490)
                     /locus_tag="VIH70_06470"
     CDS             complement(228..1490)
                     /locus_tag="VIH70_06470"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8116039.1"
                     /translation="MSIGRTEFTSGLRVVTERMPGVRSVALGFWVLAGSRDEPPPISG
                     SSHFLEHLLFKGTKTRSARDIAEAFDAVGGEVNAFTAKEYTAFYARVRDRDLPMAVDH
                     LADMLQHSVLRAADLDAERQVILEEINQHEDDPGDLVHDLFTETLWPGHPLGRPILGT
                     KQSIQAAGRDQVRRFYRRRYVAGNVVVVAAGNLRHEELLDLVAEHMEPGDVRAEGPSA
                     WNLRTAGNAPSPSGRSLVNKRKTEQAHICVGTSGLSRIDPDRFAFGVVNNALGGGMSS
                     RLFQEVREKRGLAYSVYSYHAMYAEAGVFCAYAGTTPARAKEVLAIMRAELEDVANKG
                     LGPDEFERAKGHMKGSLVLSLEDPGGRMSRLGKSEIGHGETLTVNQLLGRIDAVTPED
                     AARAAERVLSQPMTVTVLGPFSNKDAIA"
     gene            complement(1494..>2925)
                     /locus_tag="VIH70_06475"
     CDS             complement(1494..>2925)
                     /locus_tag="VIH70_06475"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY8116040.1"
                     /translation="EAAMAEIKDLLKAHLLETWGAESYAARQSEISPAWKDLQKKVMR
                     ARVVEHGVRLDGRGPKDIRPLSAEVGLIPRAHGSSLFNRGDTQALNVTTLGMLRMTQM
                     IDTLDPEESKRYIHHYNFPPFSTGEVGRIGSPRRREIGHGALAERALVPVVPTEEEFP
                     YALRLVSDILSSNGSTSMASVCASTLSLMDAGVPIKAPVAGIAMGMIAEGDTFVTLTD
                     ILGAEDALGDMDFKVAGTSEFVTAIQLDMKVTGLPGEVLAGALDQAKEARLQILDVLR
                     DAIPEPRTEVNPKAPRILTIKIPVDKIGEVIGPKGKKINEIVALTGADIDIQDDGTVF
                     VGAREAGGADEAARIIDEIANPRPISVGEHFTGTVVNTTTFGAFVNLVPGRDGLIHIS
                     KLGKGKRIANVEDAVKVGDTIEVEVQDIDDRGKISLKPVGEEWAIPEGMEQEAQDRPP
                     RRDRDRDRRPRDRDRRPREREPRDPA"
BASE COUNT          401 a         1050 c          987 g          487 t
ORIGIN      
        1 gtccgggtgc gtgaagtcga tcgcgacctc ggcgttggcc tcgagcagcc gatcgagctc
       61 gtcggagacc ggcgcgtcga tctcggggtg tccgatcatc tgcccgatct tctgcccgat
      121 cttcgagcga tcgatcgcgg caacgagcgc gaagtcgtgc gagtccgcca gggcccggca
      181 gaccatcagc cccatccggc cgcaggcacc gatcacgccg acgcggatca cgcgatcgcg
      241 tccttgttcg agaagggacc gaggacggtg accgtcatcg gttgcgagag tacccgttcg
      301 gcggcgcgcg ccgcgtcctc cggggtcacg gcatcgatgc gaccgaggag ctggttcacc
      361 gtcagggtct ctccgtgccc gatctccgac ttacccagcc gcgacatgcg ccccccgggg
      421 tcctcgagcg acaacacgag cgaccccttc atgtggccct tcgcgcgctc gaactcgtcg
      481 ggaccgaggc ccttgttggc cacgtcctcg agctcggccc gcatgatcgc gaggacctcc
      541 ttcgcgcgcg ccggcgtggt gccggcgtag gcgcagaaca cgcccgcctc cgcgtacatc
      601 gcgtggtagg agtacacgct gtacgcgagc ccgcgcttct cgcgcacctc ctggaacaac
      661 cgggagctca tccccccgcc gagcgcgttg ttcacgaccc cgaacgcgaa ccggtccggg
      721 tcgatcctcg acagaccgct ggtccccacg cagatgtggg cctgctcggt cttgcgcttg
      781 ttgaccagcg agcgccccga tgggctgggg gcgttgccgg cggtccgcag gttccaggcg
      841 ctcgggccct cggcgcgcac gtcgcccggc tccatgtgct cggcgacgag gtcgagcagc
      901 tcctcgtgcc gcaggttccc cgccgcgacg acgaccacgt tgccggcgac gtagcgcctg
      961 cgatagaagc gccgcacctg gtcgcgcccg gcggcctgga tgctctgctt ggtgccgagg
     1021 atcgggcggc ccagcgggtg cccgggccac agcgtctcgg tgaacaagtc gtgcacgaga
     1081 tcgcccggat cgtcctcgtg ctggttgatc tcctcgagga tcacctggcg ctcggcgtcc
     1141 agatcggccg cccgcagcac cgaatgctgc agcatgtccg cgaggtggtc gacggccatc
     1201 ggcaggtccc ggtcgcgcac gcgcgcgtag aacgccgtgt actccttggc ggtgaacgcg
     1261 ttgacctcgc cgccgaccgc gtcgaacgcc tcggcgatgt cgcgcgcgct ccgggtcttc
     1321 gtccccttga acagcaggtg ctcgaggaag tggctcgacc ccgagatcgg cggcggctcg
     1381 tcacgcgagc cggccagcac ccagaagccg agggccaccg agcggacgcc gggcatccgc
     1441 tcggtgacga cgcgcaggcc ggaggtgaac tccgtacgtc cgatcgacat cggctacgcg
     1501 ggatcgcgcg gttcgcgctc gcgtgggcgt cggtcgcggt cgcgcggccg gcgatcgcgg
     1561 tcgcgatcgc gacgcggagg ccggtcctgc gcctcctgct ccatcccctc ggggatcgcc
     1621 cactcctcgc ccacgggctt gagcgagatc ttcccacgat cgtcgatgtc ctggacctcg
     1681 acctcgatcg tgtcgccgac cttgaccgcg tcctcgacgt tcgcgatccg ctttcccttg
     1741 ccgagcttgg agatgtggat caggccgtcg cggcccggca ccaggttcac gaacgcaccg
     1801 aacgtggtcg tgttgaccac ggtgcccgtg aagtgctcgc cgacgctgat cggacgcggg
     1861 ttcgcgatct cgtcgatgat acgagccgcc tcgtccgccc cgccggcctc gcgagcaccg
     1921 acgaacacgg tcccgtcatc ctggatgtcg atgtcggcgc cggtcagggc gacgatctcg
     1981 ttgatcttct tgcccttggg accgatcacc tcgccgatct tgtccaccgg gatcttgatc
     2041 gtgaggatcc gcggggcctt cgggttgacc tcggtccgcg gctccgggat cgcgtcgcgc
     2101 agcacgtcga ggatctggag tcgtgcctcc ttcgcctgat cgagcgcgcc cgcgagcacc
     2161 tccccgggga ggccggtgac cttcatgtcc agctggatcg ccgtgacgaa ctcgctcgtc
     2221 cccgccacct tgaagtccat gtcgccgagc gcgtcctcgg cgccgaggat gtcggtcagg
     2281 gtcacgaacg tgtccccctc cgcgatcatc cccatcgcga tccccgcgac cggggccttg
     2341 atcgggaccc cagcgtccat cagcgacagc gtcgaggcgc agacgctcgc catcgacgtg
     2401 gagccgttgg agctcaggat gtccgacacg agcctgagcg cgtaggggaa ctcctcctcc
     2461 gtcgggacca ccggcacgag cgcgcgctcg gcgagggccc cgtggccgat ctcgcgacgg
     2521 cgcggcgagc cgatgcggcc cacctcgccg gtggagaacg gcgggaagtt gtagtggtgg
     2581 atgtagcgct tgctctcctc cgggtccagc gtgtcgatca tctgggtcat ccggagcatc
     2641 ccgagcgtgg tcacgttcag ggcctgcgtg tcgccgcggt tgaacaggct cgaaccgtgc
     2701 gcgcgcggga tcaacccgac ctcggccgag agcggccgga tgtccttggg accgcggccg
     2761 tcgaggcgga cgccgtgctc gacgacgcgt gcccgcatga ccttcttctg caggtccttc
     2821 cacgcgggcg agatctcgga ctggcgcgcg gcgtaggact ccgcgcccca ggtctcgagc
     2881 aggtgcgcct tcaggaggtc cttgatctcg gccatcgcgg cctcg
//
LOCUS       DATBRK010000215         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1043938, whole genome shotgun sequence.
ACCESSION   DATBRK010000215 DATBRK010000000
VERSION     DATBRK010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043938"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VIH70_06480"
     CDS             <1..1060
                     /locus_tag="VIH70_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEY8116041.1"
                     /translation="GISGQVYLFKNNTDSAGNSYGCHENYLVARQGEFARMADLLIPF
                     FVTRQIWCGAGKVLHGPRGAVFCIAQRAEHIWEGVSSATTRSRPIINTRDEPHADAER
                     FRRLHVIVGDSNMSEFSSWMKVGVTDLVLRMLEANTVMRDLSLENPIRAIREISHDVT
                     CRKKVRLANGRELSAIEVQEEYYERTERFLERRGPDPQSKQLLQEWREALDALILQEP
                     ERLVRKVDWVAKQSLIENYRQRHQLPLSHPRVALLDLAYHDVNRTRGLYYVLERAGKM
                     ERAVAEKEIQRAMREPPQTTRAKLRGDFIRHAKQKRRDYTVDWVHLKLNDQAQRTVLC
                     KDPFRSVDDRVEQLISHM"
     gene            complement(1112..2227)
                     /locus_tag="VIH70_06485"
     CDS             complement(1112..2227)
                     /locus_tag="VIH70_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEY8116042.1"
                     /translation="MRLRHATVLRIRSERPGVLELDVVVDDDPADEPPVAEPAPAVAF
                     TQLVGRVEVGGRVLVNTTAVALGLGTGGFHLVVAVDDQTPTELDHPGRVMKARYTPLQ
                     VAVESVEETDRETLERSVGLGGRPVVCAPLHSMIGPIAAGARAAGADRIAYVMSDGAA
                     LAGRLSNLVPRLRAAGLLDGFVTSGQAFGGELEAVTIWTGALAAAELVGADVVIVADG
                     PGNLGTDTTWGVSALASGHALNAAEALGGRPVAALRVSFADARERHRGVSHHSLTILD
                     RVCRVAADVAVPVLDERERDLVWNSLRERGLEERHQLVEVDGRPALDELERAGVEIDS
                     MGRTESKDPAFFLAAGAAGILAGRMAAGSRRWRRGAG"
     gene            2281..>2660
                     /locus_tag="VIH70_06490"
     CDS             2281..>2660
                     /locus_tag="VIH70_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY8116043.1"
                     /translation="MHPLERLVNLVALLLESRTPLTFEQIRSKLAEGYGHQDASSAKR
                     MFERDKDVLRDIGVPIDVVSTDAWDVEHGYTIEKDRYYLPEIAFTPEELSALFVAVRS
                     AGDVSAEEAVRKLLSGADRGILAGL"
BASE COUNT          416 a          966 c          922 g          356 t
ORIGIN      
        1 gggcatcagc gggcaggtct acctgttcaa gaacaacacc gactcggcgg gcaactcgta
       61 cgggtgccac gagaactacc tggtggcccg gcagggcgag ttcgcgcgca tggccgacct
      121 gctgatcccg ttcttcgtga cccggcagat ctggtgcggc gccggcaagg tcctgcacgg
      181 ccctcgcggc gccgtgttct gcatcgccca gcgcgccgag cacatctggg agggcgtctc
      241 gtcggcgacc acgcgctcga gaccgatcat caacacgcgc gacgagccgc acgcggacgc
      301 cgagcggttc cgccggctgc acgtgatcgt cggggactcg aacatgtccg agttcagctc
      361 gtggatgaag gtgggcgtca ccgacctggt ccttcggatg ctcgaggcga acacggtgat
      421 gcgcgacctg tcgctcgaga acccgatccg cgcgatccgc gagatcagtc acgacgtgac
      481 ctgtcgcaag aaggtccggc tggccaacgg gcgcgagctg tcggccatcg aggtccagga
      541 ggagtactac gagcgcaccg aacgcttcct ggagcgacgc ggtcccgatc cgcagtccaa
      601 gcagctgctg caggagtggc gcgaggcgct cgacgcgctg atcctgcagg agccggagcg
      661 gctggtgcgc aaggtcgatt gggtcgccaa gcagagcctg atcgagaact atcgccagcg
      721 ccaccagctg ccgctctcgc acccgcgcgt cgcgctgctc gacctcgcct accacgacgt
      781 caaccgcaca cgcggcctgt actacgtgct cgagcgcgcg ggcaagatgg agcgcgccgt
      841 cgcggagaag gagatccagc gggccatgcg ggaacccccg cagaccacgc gggccaagct
      901 ccggggggac ttcatccgtc acgccaagca gaagcgtcgc gactacaccg tggactgggt
      961 ccacctcaag ctgaacgacc aggcccagcg gacggtgctc tgcaaggatc cgttccgcag
     1021 cgtggacgat cgcgtggagc agctgatctc tcacatgtga cgtggtccgt cggccgggcg
     1081 ccgatggacg tcgacgcaca ccccggagga ttcagccggc ccctcggcgc cagcggcgcg
     1141 atcccgccgc catcctgccc gcgaggatgc ccgccgcacc cgccgcgagg aagaacgccg
     1201 ggtccttcga ctcggtgcgg cccatggagt cgatctcgac ccccgcgcgt tcgagctcgt
     1261 cgagcgccgg ccgcccgtcc acctcgacca gctggtggcg ctcctccagg cctcgctcgc
     1321 gcagcgagtt ccacacgagg tcgcgctcgc gttcgtccag gaccgggacg gcgacgtcgg
     1381 ccgccacccg gcacacgcgg tcgaggatcg tgagcgagtg gtgcgagacc ccgcggtggc
     1441 gttcgcgcgc gtcggcgaac gagacgcgga gcgccgccac ggggcgcccg ccgagcgcct
     1501 ccgccgcgtt cagggcgtgg ccgctcgcga gcgcgctcac gccccacgtc gtgtcggtgc
     1561 cgaggttgcc cggcccgtcg gccacgatca cgacgtcagc gccgacgagc tcggcggcgg
     1621 cgagcgcgcc ggtccagatc gtcacggcct cgagctcgcc cccgaaggcc tggccgctcg
     1681 tcacgaagcc gtccagcagc cccgcggccc gcaggcgcgg gacgaggttc gagagccgac
     1741 ccgcaagcgc ggctccgtcc gacatcacgt aggcgatccg atcggcgccg gccgcccgcg
     1801 ccccggcggc gatcggaccg atcatcgaat gcaacggcgc gcacacgacc ggcctgccgc
     1861 ccaggcccac cgagcgctcg agcgtctcgc gatcggtctc ctcgacgctc tccaccgcca
     1921 cctgcaacgg cgtgtagcga gccttcatca ctcgtcccgg atggtcgagc tcggtcgggg
     1981 tctggtcgtc gacggccacg acgaggtgga agccgcccgt ccccagaccg agcgccaccg
     2041 ccgtggtgtt cacaaggacg cggccgccga cctccacgcg accgacgagc tgggtgaacg
     2101 ccacggccgg cgccggttcg gcgaccggcg gttcgtcggc cggatcgtcg tccacgacca
     2161 cgtcgagctc gagcacgccc ggacgttcgc tccggatgcg caggacggtg gcgtgacgca
     2221 ggcgcacgct cgctcgcgtc ggggggcccg gcgacatggc ggaagtatga tgccgcggcc
     2281 atgcacccgc tcgaacgcct ggtgaacctc gtcgccctgc tgctcgagtc ccggacgccg
     2341 ctcacgttcg aacagatccg gagcaagctg gcggaggggt acgggcatca ggacgcgtcg
     2401 tccgccaagc gcatgttcga gcgcgacaag gacgtcctgc gcgacatcgg ggtgccgatc
     2461 gacgtggtct cgaccgacgc gtgggacgtc gagcacggat acacgatcga gaaggaccgc
     2521 tactacctcc ccgagatcgc cttcacgccg gaggagctct cggcgctgtt cgtcgcggtg
     2581 cgttcggcgg gcgacgtctc ggcggaggag gccgtccgca agctgctctc gggcgccgac
     2641 cgcgggatcc tggccggcct
//
LOCUS       DATBRK010000216         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_470230, whole genome shotgun sequence.
ACCESSION   DATBRK010000216 DATBRK010000000
VERSION     DATBRK010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470230"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1278)
                     /locus_tag="VIH70_06495"
     CDS             complement(82..1278)
                     /locus_tag="VIH70_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8116044.1"
                     /translation="MGFAPTELDFIADPYPAYATLREREPVRYDEATDHWLISRYEDV
                     NALLRDRRVGRTYRHLASHAEMGHPDPPEWHAPFWDLINAGILDMEPPDHSRVRRLVA
                     KAFTPRFVEGLRPRVQAIMDGLVDGLDGGSEFDLLRVAEPLPVTVIAEMLGVPENDRH
                     QLRPWSADICKMYELNPSAEMQHDAVRASNEFSAYLHELSRERRRDPGEDLISKLALV
                     VDEGEQLTEDELVGTCVLLLNAGHEATVNSTLLGWWSLFRNPDRLRELRADRTRLPNA
                     IEELLRFDTPLQMFERWVLEPFEIRGVRIPRGAELGLLFGSANRDPSVFDRPDELDLT
                     REPNPHLSFGAGIHFCLGAPLGRQELLVSFATLMDRFPNLELVEEPRWKPNYVIRGLQ
                     GLLVRP"
     gene            complement(1338..1700)
                     /locus_tag="VIH70_06500"
     CDS             complement(1338..1700)
                     /locus_tag="VIH70_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116045.1"
                     /translation="MGMTGTMKGYGSAARFKAEAAGHRVKDRMLEKRLDRSSQEAERL
                     RLENQLLRDEVEESRTEHHRILDLLEERMEHEDQKEHSHKGRWMLFLLALGGGVYALV
                     HRMRDDGTTWDTGSSATA"
     gene            complement(1769..3214)
                     /locus_tag="VIH70_06505"
     CDS             complement(1769..3214)
                     /locus_tag="VIH70_06505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8116046.1"
                     /translation="MTDSVADHPGAMVSHDALLTSVPAPALRAFDERFHGRLILPTDR
                     GYDEARSIWNGAIDRRPGLVARCADASDVRSAVRFAREHDLRMSVRSGGHGVAGLALC
                     DDGLVVDLSALKGVRVDEAAGLAEVEAGITLGELDRATQAFGLAVPAGIVTHTGVAGL
                     TLGGGIGWLTRKHGLSIDNLVGADVVTADGELVAASEDDHPDLFWGLRGGGGNFGVVT
                     SFRFRAHPIGPTVLAGPMLFPLERGPEVMQTYRGWAAEAPEELTTVLNVRRAPAAPWI
                     PVPLHGVPVWMVVACWCGPIEAGDPVMAPIRALGPMLDLCAPKPFVEHQAMFDGAVTP
                     GWHYYWKSVELDELTEPAVAAIVDHTERITSPRSYAVAFQLGGAMSRVGEMDTAYTGR
                     SAAFDVNINGVWLPEEAEDAPDHVAWTRSLFGELEPMARGVYVNFLGDEGTDRVRAAY
                     GAEKFGRLAELKARRDPTNLFRSNQNIAPAS"
BASE COUNT          490 a         1208 c         1165 g          496 t
ORIGIN      
        1 acgatcgcgg gggcgacgag cggctcggtc ggttcgctca gcaggtacag cggcatggat
       61 cgaggatagc cctcggtcgc ctcagggacg gacgagcagc ccctggaggc cgcggatcac
      121 gtagttcggc ttccagcggg gctcctcgac gagctcgagg ttcgggaacc ggtccatcag
      181 cgtcgcgaac gagaccagga gctcctgacg gccgagcggg gcgccgaggc agaagtggat
      241 gccggcgccg aacgagaggt gcgggttcgg ctcgcgggtc aggtcgagct cgtcgggccg
      301 atcgaagacc gaggggtcgc ggttcgccga cccgaacaac aggccgagct ccgcgccgcg
      361 agggatccgg acgccgcgga tctcgaaggg ttcgagcacc caccgttcga acatctgtaa
      421 cggcgtgtcg aaccgcagga gctcctcgat cgcgttcggc agccgcgtgc ggtcggcgcg
      481 gagctcgcgc aggcgatcgg ggttgcggaa cagcgaccac caaccaagca gcgtggagtt
      541 gacggtcgcc tcgtgccccg cgttcagcag gagtacgcac gtgcccacca gctcgtcctc
      601 ggtcagctgc tcgccctcgt cgaccacgag cgcgagcttc gagatgaggt cctcgcccgg
      661 gtcccgcctg cgttcgcgcg agagctcgtg caagtacgcc gagaactcgt tgctcgcgcg
      721 gaccgcgtcg tgctgcatct cggccgacgg gttcagctcg tacatcttgc agatgtccgc
      781 ggaccacggg cgcagctgat ggcggtcgtt ctcggggacg ccgagcatct cggcgatcac
      841 cgtgaccggg agcggctcgg cgacccgcag caggtcgaac tcgctcccgc cgtcgagccc
      901 gtcgacgagg ccgtccatga tcgcctgcac gcgtggtcgc agcccctcga cgaaccgcgg
      961 ggtgaaggcc ttcgcgacga gccgccgcac cctgctgtgg tccggcggct ccatgtcgag
     1021 gatcccagcg ttgatcaggt cccagaacgg cgcgtgccac tcgggcggat ccgggtggcc
     1081 catctcggcg tgcgaggcca ggtgccggta ggtgcgcccg acccgccggt cgcgcaacag
     1141 ggcattcacg tcctcgtacc gcgagatcag ccagtgatcg gtcgcctcgt cgtagcggac
     1201 cggttcacgc tcgcgcagcg tggcgtaggc cgggtacggg tcggcgatga agtcgagctc
     1261 ggtcggggcg aagcccatgg ttgcgactgt agcgccgagc gccgcagccg cgacgggcga
     1321 acgatggtcg cgccgagtca ggcggtcgcg ctcgagcccg tgtcccaggt cgtcccgtcg
     1381 tcgcgcatcc ggtggacgag cgcgtagacg ccgccgccga gcgccagcag gaagagcatc
     1441 cagcggccct tgtgcgagtg ctccttctgg tcttcgtgct ccatgcgctc ctcgagcagg
     1501 tcgagaatcc ggtgatgctc ggtccttgac tcctccacct cgtcgcgcag cagctggttc
     1561 tcgagccgga gtcgctccgc ctcctggctg gagcggtcca ggcgcttctc gagcatccga
     1621 tccttcacgc ggtggccggc ggcctcggcc ttgaagcgtg ccgcggagcc gtaccccttc
     1681 atcgtcccgg tcatgcccat cgtgcgtgcc ctcctcgtgt cggggacact gggtcgatac
     1741 ccatctgggc cgagcacgaa ccagcccctc agctggccgg cgcgatgttc tggttcgagc
     1801 ggaacaggtt ggtcgggtcc cggcgtgcct tgagctcggc cagccgtccg aacttctcgg
     1861 cgccgtacgc ggcacggacg cggtcggtgc cctcgtcgcc gaggaagttc acgtagacgc
     1921 cgcgcgccat cggctcgagc tccccgaaca gcgaccgtgt ccacgccacg tggtcgggtg
     1981 cgtcctcggc ctcctccggg agccacacgc cgttgatgtt cacgtcgaac gccgccgatc
     2041 gcccggtgta cgccgtgtcc atctccccga cccgcgacat cgcgccgccg agctggaacg
     2101 cgacggcgta cgagcggggc gaggtgatcc gctcggtgtg gtcgacgatc gcggcgaccg
     2161 ccggctcggt gagctcgtcg agctcgacgg acttccagta gtagtgccac cccggcgtca
     2221 ccgcgccgtc gaacatcgcc tggtgctcga cgaagggctt cggcgcgcag aggtcgagca
     2281 tcggcccgag ggccctgatc ggtgccatca cggggtcccc ggcctcgatc ggaccgcacc
     2341 agcaggcgac caccatccag accggcacac cgtgcagcgg cacggggatc cacggcgccg
     2401 ccggcgctcg gcggacgttc agcaccgtcg tgagctcctc tggcgcctcc gccgcccatc
     2461 cgcgatacgt ctgcatgacc tcgggaccgc gttcgagcgg gaagagcatc ggtcccgcga
     2521 gcaccgtcgg cccgatcgga tgcgctcgga agcggaacga cgtgaccacg ccgaagttgc
     2581 cgccgccgcc ccggaggccc cagaacaggt ccggatggtc gtcctcgctc gcggcgacca
     2641 gctccccgtc cgccgtgacg acgtcggccc cgacgaggtt gtcgatgctc aggccgtgct
     2701 tgcgggtcag ccacccgatc ccaccgccga gcgtgagacc ggcgaccccc gtgtgcgtca
     2761 cgatcccggc cgggaccgcg agcccgaacg cctgcgtggc gcgatcgagc tcgccgaggg
     2821 tgatgccggc ctcgacctcc gccaggccgg ccgcctcgtc gacccggaca cccttcaacg
     2881 cggacaggtc cacgaccagc ccgtcgtcgc agagcgcgag gccagccacg ccgtgaccgc
     2941 cgcttcgcac cgacatccgc aggtcgtgct cgcgtgcgaa ccgcaccgcg ctccggacgt
     3001 ccgatgcgtc cgcgcaccgg gcgacgagcc ccggcctccg gtcgatcgcg ccgttccaga
     3061 tcgagcgcgc ctcgtcgtac ccccggtccg tgggaaggat cagccggccg tggaagcgct
     3121 cgtcgaacgc tcgaagcgcc ggcgccggca ccgaggtcag gagcgcgtcg tgggacacca
     3181 tcgctccggg atggtccgcc acggagtccg tcacgtcgaa tccggtcaga gcgtcggctt
     3241 ggtgaccatg aggaacacta tggcgatcac gtcgaccgcg agcacgatcc ccaccgtggt
     3301 tccgcgtttc gccagcgcct ggaactcggg cgagccggcg ccggaggtct cgagcgccg
//
LOCUS       DATBRK010000217         6791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2490871, whole genome shotgun sequence.
ACCESSION   DATBRK010000217 DATBRK010000000
VERSION     DATBRK010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490871"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VIH70_06510"
     CDS             complement(<1..230)
                     /locus_tag="VIH70_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8116047.1"
                     /translation="MTRVWTHGNWVVKAGTEAAFEEAWTRLARAAMRAYEGEPPILLR
                     DREHPNVFKTFGAFPSIEAIEAFRASDLFRGAV"
     gene            complement(239..1198)
                     /gene="deoC"
                     /locus_tag="VIH70_06515"
     CDS             complement(239..1198)
                     /gene="deoC"
                     /locus_tag="VIH70_06515"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY8116048.1"
                     /translation="MSQAAEIERSRPGLALYAPKDASRLLARVPAVDAVGLEERAASL
                     AKRSIKRDSKLWALDLAIRCMDLTTLEGTDTVGKIIAMCAKAIRPNPVDPSIPSVAAV
                     CLYPHLVPVALGQLNGTNVRVASVAGAFPAGLGPLGARLAEIREVVDLGAHEVDIVLN
                     RSYFLGGQYAQCFEEIVGAREAAGNAHLKVILETGELGSYDRIRQASVLAMAAGADFI
                     KTSTGKIGVNATLPAALCMMEAARDFFRETGRPVGVKVAGGVRASKQAIQYLVLVYET
                     LGPEWMRPERFRIGASTLLNDVLMQIDKERTGRYQGADYYTID"
     gene            1300..1710
                     /locus_tag="VIH70_06520"
     CDS             1300..1710
                     /locus_tag="VIH70_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8116049.1"
                     /translation="MRKLVFVLAATCALGLTACSSDDGGGATGAETGATGTTENGDCV
                     DLTGEGANFTITISDFAFDPNCFTASASQGISIENQDAADHTFTITGTDVDVPIAAGQ
                     TFNGEPISGAVSPGTYDFLCTLHPTMTGQVTIVE"
     gene            complement(1717..2613)
                     /gene="era"
                     /locus_tag="VIH70_06525"
     CDS             complement(1717..2613)
                     /gene="era"
                     /locus_tag="VIH70_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY8116050.1"
                     /translation="MTQPKEGFHSGFVAVIGRPNTGKSSLVNALVGRKVAIVSDKPQT
                     TRRGMRAVLSREDYQVVFTDTPGFHKPRTLLGSHLNDLVGEATRGVDAVVQVVDASAR
                     VGRGDASVFERQLRDFGGPKICAVNKLDLVRNREEVPQLEAAAALGAWDEIVPVSAKT
                     GRGVGVLLDLVIDRLPEGPAYYPTDTVTDEPLEVRLAEIVREKALAVARQEVPHSIAV
                     QVEELEESDSLTKIHASLIVERDSQKGIVIGRGGQTLKTIGSRAREEIERVLGHQVFL
                     DLHVKVMKEWQRDPKALQRLGF"
     gene            complement(2610..3020)
                     /gene="cdd"
                     /locus_tag="VIH70_06530"
     CDS             complement(2610..3020)
                     /gene="cdd"
                     /locus_tag="VIH70_06530"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY8116051.1"
                     /translation="MSAPTERIDELLAAARHAMERGSYAPYSNFNVGAAVLAEDEVFA
                     GANVENASYPMSVCAERNAVAAAVNSGKRSIDAVAVVTRAEELTPPCGGCRQVLNEFG
                     PDMTVTVESGTGERAAWRLTEILPHAFGPSNLGK"
     gene            complement(3017..4303)
                     /locus_tag="VIH70_06535"
     CDS             complement(3017..4303)
                     /locus_tag="VIH70_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY8116052.1"
                     /translation="MTAADWALLGVVVVLIGVSAVFAAAETFITRVSKVRAYRLQEEG
                     RRGAASLVKIVENPPPYLNVVLLLMLAVHLAGTTVATVVAVRTIGDLGELIATVVMTF
                     LLFVFAEVAPKTYAVLQTDRVALRLAPATVALTRVIGPLTSGLIKLSNVVMPGKGLPE
                     GPFATEEEIKAMASVASDEEQIEEGEKELIHSIFEFGDTIVREVMLPRPDITAIEADK
                     ALRDVQALVLQHGYSRIPVFEDDLDQVTGIVYAKDVLKALHQGKHDMPLAEIVRRAHF
                     VPESKKVADLLREMQKEKFHIALVTDEYGSVVGLITLEDLLEELVGEITDEYDTEEPE
                     LEQVADGVFRVDGKLSIDEVNELLDVELPDEEWDTVGGLMLGLMGSIPEEGEEVSFQN
                     LRFTAEKVNGRRISKVLITREAQPEPTTEEQEVRAE"
     gene            complement(4300..4740)
                     /gene="ybeY"
                     /locus_tag="VIH70_06540"
     CDS             complement(4300..4740)
                     /gene="ybeY"
                     /locus_tag="VIH70_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY8116053.1"
                     /translation="MADDDQPPLIALDDRQDADVDLEGLMTLARATLLGEGIADAELS
                     ISFVTEDEIAGLHERYLHEAGPTDVLSFPLDDEAGDDGLRQLGDVVIAPAVATRNNPD
                     DPAAELRLLLVHGILHLLGHDHMDDGERAEMWARQERYSGVRVR"
     gene            complement(4733..5659)
                     /locus_tag="VIH70_06545"
     CDS             complement(4733..5659)
                     /locus_tag="VIH70_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY8116054.1"
                     /translation="MVALLGQRDELLKLVEAAFDSEIVVRGNEITIAGPEVEVERVAL
                     LFEELITLLERGHELNASSVGRSIELIKGEEATPTQVLGDVFLTGRGRGIAPKTLGQK
                     RYVDAMRSGTVTFAIGPAGTGKTYLAVATAVRALELKQVSRLILTRPAVEAGERLGFL
                     PGTLYEKIDPYMKPLYDALFEMMGADALPRLMERGTVEVAPLAYMRGRTLNDSFIILD
                     EAQNTTPEQMKMFLTRLGFGSKAVVNGDVTQIDLPTGQTSGLVIVQEILRGIEGIEVA
                     HLGARDVVRHKIVQDIVEAYRVFGEQRARADG"
     gene            complement(5842..6189)
                     /locus_tag="VIH70_06550"
     CDS             complement(5842..6189)
                     /locus_tag="VIH70_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY8116055.1"
                     /translation="MSSSCPFCRVVSGEDPAEILHSTETVLAFRDLRPQAPTHLLLIP
                     KEHIADVRDLEQRHGGMLHDLFQAAAHLAKTEGVDASGWRLVANVGRDAGQSVFHLHL
                     HLLGGRPMRWPPG"
     gene            complement(6186..>6791)
                     /locus_tag="VIH70_06555"
     CDS             complement(6186..>6791)
                     /locus_tag="VIH70_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY8116056.1"
                     /translation="RRSIFGTVMTAHSCGTCQGTGEEIVSSCEACQGRGRVAAHATVP
                     IDVPAGVTDGMELRIGGAGHAGRAGGPPGDLYLSIHVDEDPVFERHGPDVFAVLEVPM
                     VQAALGGEVQVATLDGTERVGIDPGTESGTTVRLKGKGIPNLGRGGRGDLFLTIAVQT
                     PRDLSRDQRRLVEELARLRGEEAGKRTSTEGTLRRPGTQGR"
BASE COUNT          998 a         2467 c         2242 g         1084 t
ORIGIN      
        1 accgccccac ggaacaggtc agaggcccgg aaggcctcga tcgcctcgat gctcggaaac
       61 gcgccgaacg tcttgaacac gttcggatgc tcgcggtccc tcaacaagat cgggggctcg
      121 ccctcgtacg cccgcatcgc ggcgcgcgcc agacgggtcc aggcctcctc gaacgcagcc
      181 tcggtccccg ccttgaccac ccagttgccg tgcgtccaca ccctcgtcat cgccctgcct
      241 agtcgatcgt gtagtagtcg gcaccctggt agcggccggt ccgctccttg tcgatctgca
      301 tcagcacgtc gttcagcaac gtcgacgccc cgatccggaa ccgctcgggc ctcatccact
      361 ccggcccgag cgtctcgtac accagcacga ggtactggat cgcctgtttc gaggcccgga
      421 cgccgccggc gaccttgacg cccacggggc gacccgtctc gcggaagaag tcccgagccg
      481 cctccatcat gcagagcgcg gcggggagcg tcgcgttcac gccgatcttg ccggtcgagg
      541 tcttgatgaa gtcggctccg gcggccatcg cgagcaccga cgcctggcgg atccggtcgt
      601 acgacccgag ctcgccggtc tcgaggatca ccttgaggtg cgcgttcccc gcggcctcgc
      661 gcgcgccgac gatctcctcg aagcactgtg cgtactgccc gcccaggaag tacgaacggt
      721 tcagcacgat gtcgacctcg tgcgcgccaa ggtcgacgac ctcgcggatc tcggccaggc
      781 gggcgccgag cggcccgaga ccggccggga aggcgcccgc gacgctcgcg accctcacat
      841 tcgtgccatt cagctgcccg agcgcgaccg gcacgagatg cgggtacaag cagacggccg
      901 ccaccgaggg gatcgagggg tcgaccggat tgggccggat ggccttcgcg cacatcgcga
      961 tgatcttgcc caccgtgtcg gtgccctcca gggtggtcag gtccatgcac cggatcgcca
     1021 ggtcgagcgc ccacagcttc gaatcgcgct tgatcgaccg cttagcgagg gaggccgcgc
     1081 gctcctcgag cccgacggcg tccaccgcgg ggacccgagc gaggagccgg gacgcgtcct
     1141 tcggcgcata cagcgccaga ccgggacgcg agcgttcgat ctcggctgcc tgggacatcg
     1201 cccgccatgg tacggcgagg gctcgcctcg cggctctctc cgcgcccggc gccaacgcgg
     1261 ccgcggctat cctctgcggg accgatctcg ggaggcggga tgaggaagct cgtgttcgtg
     1321 ctggccgcga cgtgcgccct cgggctcacg gcgtgctcgt cggacgacgg tggcggagcc
     1381 accggcgcgg agaccggggc gaccgggacg accgagaacg gcgactgcgt cgacctgacg
     1441 ggcgagggcg cgaacttcac gatcacgatc tcggacttcg cgttcgaccc gaactgcttc
     1501 accgccagcg cgtcccaggg catctcgatc gagaaccagg atgccgccga ccacaccttc
     1561 acgatcaccg ggaccgatgt cgacgtcccg atcgccgccg gacagacgtt caacggcgag
     1621 ccgatctcgg gagccgtgag cccggggacc tacgacttcc tgtgcaccct ccatccgacg
     1681 atgacggggc aggtcacgat cgtcgagtag gacgcgtcag aaaccgagcc gctggagggc
     1741 cttggggtcg cgctgccact ccttcatcac cttgacgtgc aggtcgagga agacctggtg
     1801 tccgaggacg cgttcgatct cctctcgcgc gcgcgagccg atcgtcttga gggtctggcc
     1861 gcccctgccg atcacgatgc ctttctggga atcgcgctcg acgatcaagg acgcgtggat
     1921 cttcgtcagg gagtccgact cctcgagctc ctccacctgc acggcgatcg agtgggggac
     1981 ctcctgccgg gcgacggcga gcgccttctc gcggacgatc tccgccaggc gcacttcgag
     2041 cggctcgtcc gtgaccgtgt cggtcgggta gtacgcgggg ccctcgggca ggcggtcgat
     2101 caccaggtcg aggagcacgc cgaccccgcg tcctgtcttg gccgagaccg ggacgatctc
     2161 gtcccaggct cccagggccg ccgccgcctc gagctgcggg acctcctcgc ggttgcgcac
     2221 cagatcgagc ttgttcaccg cacagatctt gggcccgccg aagtcgcgca gttggcgttc
     2281 gaacacggag gcgtcgcccc ggcctacgcg ggccgaggcg tcgaccacct gcaccaccgc
     2341 gtcgacgccc cgcgtcgcct cgccgacgag gtcgttgaga tgcgatccga gcagcgtccg
     2401 cggcttgtgg aaccccgggg tgtcggtgaa cacgacctgg tagtcctccc tcgacagcac
     2461 ggcccgcatg ccgcggcgcg tcgtctgcgg cttgtcggag acgatcgcga ccttgcgccc
     2521 gacgagcgcg ttcacgaggg aagacttgcc ggtgttcggc cgcccgatca cggcgacgaa
     2581 cccgctgtgg aagccctcct tcggttgcgt cacttcccga ggttggaggg tccgaacgcg
     2641 tgtggcagga tctcggtgag tcgccaagcc gctcgttcgc cggtccccga ctccaccgtc
     2701 accgtcatgt cggggccgaa ctcgttcagg acctgccggc acccgccgca cgggggtgtg
     2761 agctcctcgg cgcgcgtgac gaccgcgacc gcgtcgatcg accgcttgcc ggagttgacg
     2821 gcggcggcga ccgcgttgcg ttcggcacac accgacatcg ggtacgaggc gttctcgacg
     2881 ttcgcgcccg cgaacacctc gtcctcggcc aggaccgcgg cgccgacgtt gaagttcgag
     2941 tacggcgcgt aggaaccgcg ttccatcgca tggcgggccg cggccagcag ctcgtcgatg
     3001 cgctcggtcg gtgcgctcat tcggctcgca cctcctgttc ctcggtcgtg ggctcgggct
     3061 gcgcctcccg tgtgatcagg accttcgaga tccggcggcc gttgaccttc tccgccgtga
     3121 accgcaggtt ctgaaacgag acttcctcgc cctcctccgg gatcgagccc atgaggccga
     3181 gcatcagccc cccgaccgtg tcccactcct cgtcggggag ctcgacgtcc aagagctcgt
     3241 tcacctcgtc gatcgagagc ttgccgtcca cgcggaacac gccgtcggcc acctgctcga
     3301 gctcgggctc ctcggtgtcg tactcgtcgg tgatctcgcc caccagctcc tcgagaaggt
     3361 cctcgagcgt gatcagtccg acgacgctgc cgtactcgtc ggtcacgagc gcgatgtgga
     3421 acttctcctt ctgcatctcg cggagcaggt cggccacctt cttggactcg ggcacgaagt
     3481 gcgccctgcg cacgatctcg gccagcggca tgtcgtgctt gccctggtgg agcgccttga
     3541 gcacgtcctt cgcgtagacg atgccggtca cctggtccag gtcgtcctcg aagaccggga
     3601 tccgcgagta tccgtgctgg agcaccagcg cctgcacgtc gcggagcgcc ttgtccgcct
     3661 cgatcgccgt gatgtccggg cgggggagca tcacctcgcg cacgatcgtg tcgccgaact
     3721 cgaagatcga gtggatgagc tccttctcgc cctcctcgat ctgctcctcg tcggaggcga
     3781 ccgacgccat cgccttgatc tcctcctcgg tcgcgaacgg cccctcgggc aggcccttgc
     3841 cgggcatcac gacgttcgag agcttgatga ggccggaggt gagggggccg atcacgcgcg
     3901 tgagcgcgac cgtcgcgggg gccagccgga gcgcgacccg atcggtctgc agcaccgcgt
     3961 aggtcttcgg cgcgacctcc gcgaacacga acaggaggaa ggtcatgacg accgtcgcga
     4021 tgagctcgcc gaggtcgccg atcgtgcgca cggccaccac ggtggccacc gtggtccccg
     4081 cgagatgcac ggccagcatc aacaggagga cgacgttcag gtacggcggc gggttctcga
     4141 cgatcttgac cagggaggcg gcgccgcgcc tgccttcctc ctgcagccgg taggcgcgca
     4201 ccttgctgac ccgcgtgatg aaggtctcgg cggccgcgaa cacggccgag accccgatga
     4261 ggacgacgac gaccccgagc aacgcccagt cggcggccgt caccggaccc gcacccccga
     4321 gtagcgctcc tggcgcgccc acatctcggc ccgctcccca tcgtccatat ggtcgtgccc
     4381 gagcaggtgc aggatgccgt gcacgagcag gagccggagc tcggccgccg gatcgtccgg
     4441 gttgttccgg gtagcgaccg ccggcgcgat caccacgtcc ccgagctggc gcagcccgtc
     4501 atcgccggct tcgtcgtcca gcgggaacga gagcacgtcg gtcgggccgg cctcgtgcag
     4561 gtagcgctcg tggagcccgg cgatctcgtc ctcggtcacg aaggagatcg acagctcggc
     4621 atcggcgatc ccctcgccga gcagggtcgc gcgtgcgagc gtcatgaggc cttcgaggtc
     4681 gacgtcggcg tcctgccggt cgtcgagcgc gatgagcgga ggttggtcat cgtcagccat
     4741 cggcgcgggc acgctgctct ccgaagacgc ggtacgcctc cacgatgtcc tgcacgatct
     4801 tgtgccgaac gacgtcgcgg gccccgaggt gggccacctc gatgccctcg atcccccgca
     4861 ggatctcctg cacgatcacc agtccggagg tctgtccggt cggcaggtcg atctgcgtga
     4921 cgtcgccgtt cacgaccgcc ttcgagccga acccgagccg cgtgaggaac atcttcatct
     4981 gctcgggtgt cgtgttctgc gcctcgtcca ggatgatgaa ggaatcattc aacgttcttc
     5041 cccgcatata cgcgagcggc gcgacctcga ccgtcccgcg ttccatgagg cgcggcaaag
     5101 cgtcggcgcc catcatctcg aacagggcgt cgtacagcgg cttcatgtag gggtcgatct
     5161 tctcgtacag cgtccccggt aggaacccga gccgctcgcc ggcctcgacc gcgggccggg
     5221 tcaggatcag ccgcgagacc tgcttcagct cgagcgcccg caccgcggtc gccacggcga
     5281 ggtacgtctt gcccgtcccg gcgggaccga tcgcgaacgt gaccgtccca ctgcgcatcg
     5341 cgtccacgta gcgcttctgt ccgagtgtct tgggcgcgat cccgcgtccc cgcccggtga
     5401 ggaacacgtc gccgagcacc tgcgtcggcg tggcctcctc gcccttgatg agctcgatcg
     5461 accggccgac cgaggaggcg ttcagctcgt ggccgcgctc gagcagcgtg atcagctcct
     5521 cgaacagcag cgcgacgcgc tcgacctcga cctccggacc ggcgatcgtg atctcgttcc
     5581 cgcggaccac gatctcagaa tcgaaggcgg cctcgaccag cttcagcagc tcgtcgcgct
     5641 gaccgaggag ggcaaccatg ctctggtccc ccgggaccaa gatcttgacc tgcgtggtgg
     5701 ctctcgccaa acggctcgct ctggggtggt gccccgctcc ttctcgatcg gaaccctcgg
     5761 acccggtcgc tttcggacga tccgcgttgg gtccggattc tacccccggg cgcgcgccgt
     5821 cacgcgctcg cgccctcgac atcagccggg cggccagcgc atcggccgcc ctccgaggag
     5881 atggaggtgc aggtggaaca cgctctgtcc ggcgtcgcgg cccacgttgg cgaccagccg
     5941 ccacccggaa gcatcgacgc cctcggtctt ggcgaggtgc gctgcggcct ggaacaggtc
     6001 gtggagcatc ccgccgtggc gttgctcgag gtctcggacg tccgcgatgt gctccttcgg
     6061 gatcaacaac aggtgcgtgg gggcctgcgg ccgcaggtcc cggaacgcga gcacggtctc
     6121 ggtcgagtgc aggatctccg ccgggtcctc gcccgagacg acgcggcaga acgggcagct
     6181 cgagctcatc gaccctgcgt cccgggacgc cgcaaggttc cctcggtgct cgtccgcttc
     6241 cccgcctcct cgccgcggag ccgcgcgagc tcctcgacca acctgcgttg atccctggac
     6301 agatcccggg gggtctgcac cgcgatcgtc aggaacaggt cgccccggcc cccgcgaccc
     6361 aggttcggga tccccttgcc cttcaggcgg acggtggtcc cggactcggt ccctggatcg
     6421 atcccgacgc gttcggtgcc gtccaacgtc gcgacctgga cctcgccgcc gagcgcggcc
     6481 tgcaccatcg ggacctcgag caccgcgaac acatcggggc cgtgccgttc gaacaccggg
     6541 tcctcgtcga cgtggatcga caggtacagg tcccccggcg ggcccccggc acgaccggcg
     6601 tgccccgcgc cgccgatcct gagctccatc ccgtcggtga cgcccgcggg cacatcgatc
     6661 ggcacggtgg cgtgcgccgc gacgcggccc cgtccctggc acgcctcgca cgacgagacg
     6721 atctcctccc ccgtgccctg acaggtgccg cacgagtgcg cggtcatcac ggtcccgaag
     6781 atgctccggc g
//
LOCUS       DATBRK010000218         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1480401, whole genome shotgun sequence.
ACCESSION   DATBRK010000218 DATBRK010000000
VERSION     DATBRK010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480401"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VIH70_06560"
     CDS             <1..526
                     /locus_tag="VIH70_06560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY8116057.1"
                     /translation="VTRGILEILDERELRGVLAHELSHVANRDILIGSIAAAIGMSIT
                     FLARFALWFGSGNDRNGIGIVGLLLAWVLAPIAAAIIQMAVSRSREYQADESGAILSR
                     DPDALASALRKLENAAGKVPVPASVSPAEAHLFIVNPFRGRQASMSLANLFSTHPPTA
                     ERVARLEQIKREIA"
     gene            complement(547..1215)
                     /locus_tag="VIH70_06565"
     CDS             complement(547..1215)
                     /locus_tag="VIH70_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8116058.1"
                     /translation="MSEVRAVLLDLYDTLAWTEWPTMRAELEDRFGLGEADLIRAFTA
                     TREARSTGAFGSAEGDLTAILDAAGVPSDPILVRELNDARIKAFADNGVHLWDDSVPT
                     MRELRARGVRTAIVSNCDHATRAIVDDLGLANEVDAIVLSFEVGVAKPDPGIYRAALL
                     AVGARPEEAVFVDDQAWYCEGAEALGIRSFLIRRDDADPAEGFGAAGDRDELHDLRSL
                     IDLV"
     gene            complement(1212..2561)
                     /locus_tag="VIH70_06570"
     CDS             complement(1212..2561)
                     /locus_tag="VIH70_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY8116059.1"
                     /translation="MHEYTSETDTLTGAVVDYTRRRIAHPQPLDHPVPPEDLTRSAGP
                     TITPDGIGGREALRIWEEVLAPATITTDHPSFLAFVPGAPSKASVLFDLAVSASEVYG
                     GSWLEGGGAVWAENQVLGWLAELAGMPGGSGGCFVSGGTAGNLSALVAARHAASDARG
                     GERPPRWQIACADTVHSSVASAARVMDVDLLSAPHDERGRLTGPALASALDRTDPGAA
                     FAVVASAGSTNAGVVDDLEGVAAVCRERGLWLHVDGAYGGAGLLAPSVRDRFRGIEDV
                     DSFIVDPHKWLFAPYDSCALIYREPERARAAHRQDASYLDALNATSEWNPSDYAHHLS
                     RRPRGLPLWFSLATYGTDAYRDAVERVLSITRETAEEIRRRPELELIVEPELSVVLFR
                     RLGWGPREYEAWWRTALTEQVGFVQPTSWEGEKVGRLCFVNPLTTIEDVRAILDLAA"
     gene            complement(2612..>2801)
                     /locus_tag="VIH70_06575"
     CDS             complement(2612..>2801)
                     /locus_tag="VIH70_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003780726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8116060.1"
                     /translation="NYRDFLETIPELFYASFTWMVNRGIFMTPGDEEQWTISVQHTEE
                     DIRRYVDAYAAFCAELVA"
BASE COUNT          399 a         1005 c          987 g          410 t
ORIGIN      
        1 ggtcacccgc gggatcctcg agatcctgga cgaacgcgag ctgcggggcg tgctggcgca
       61 cgagctctcg cacgtcgcca accgggacat cctgatcggt tcgatcgcgg cggccatcgg
      121 gatgagcatc acgttcctcg cacgattcgc cctgtggttc gggtcgggca acgatcgcaa
      181 cgggatcggg atcgtggggc tgctgctggc ctgggtcctc gccccgatcg ctgccgcgat
      241 catccagatg gccgtgagcc gctcgcgcga gtatcaggcc gacgagtccg gtgcgatcct
      301 gtcgcgcgac cccgacgcgc tggcgagcgc gctccgcaag ctcgagaacg cagccggcaa
      361 ggtcccggtg cccgcgtcgg tgagcccggc cgaggctcac ctgttcatcg tgaacccgtt
      421 ccgcggccgc caggcgtcca tgagcctggc gaacctgttt tcgacccacc ccccgacggc
      481 ggagcgcgtc gcgaggctcg agcagatcaa gcgggagatc gcctagctcg tttcgggcgc
      541 cctcgatcag acgaggtcga tcagcgaccg gaggtcgtgc agctcgtcgc ggtcgccggc
      601 ggcgccgaac ccctcggcgg ggtcggcgtc gtcgcgccgg atcaggaacg accggatgcc
      661 gagcgcctcg gcaccctcgc agtaccaggc ctgatcgtcg acgaagacgg cctcctcggg
      721 tcgtgcaccc acggccaaga gcgccgcccg gtagatcccg gggtccggct tggccacgcc
      781 cacctcgaac gagagcacga tcgcgtccac ctcgttcgcc agcccgaggt cgtcgacgat
      841 cgcgcgggtg gcgtggtcgc agttgctcac gatcgcggtt cggaccccgc gggcgcggag
      901 ctcgcgcatg gtcgggaccg agtcgtccca caggtggacg ccgttgtcgg cgaaggcctt
      961 gatgcgggca tcgttgagct cgcggaccag gatcgggtct gacggaaccc cggccgcatc
     1021 caggatggcg gtgaggtcgc cctcggcgct gccgaacgcg ccggtgctcc gggcctcgcg
     1081 cgtggcggtg aaggcgcgga tgaggtccgc ctcgccgagc ccgaaccgat cctcgagttc
     1141 ggcacgcatc gtcggccact cggtccatgc aagcgtgtcg tacaggtcga gcaggacggc
     1201 ccggacctcg ctcacgcggc gaggtcgagg atcgcccgca cgtcctcgat cgtcgtgaga
     1261 gggttgacga agcagagccg gcccaccttc tcgccctccc acgacgtggg ttggacgaac
     1321 cccacctgct cggtgagcgc cgtgcgccac caggcctcat actcccgcgg cccccatccg
     1381 agccttcgga acaggaccac ggacagctcc ggttccacga tgagctcgag ctcgggccgc
     1441 cggcggatct cctcggccgt ctcccgcgtg atcgacagaa cccgctcgac cgcgtcccgg
     1501 taggcgtccg tcccgtaggt cgcgagcgag aaccacaggg ggagcccgcg cggacgccgg
     1561 gagaggtggt gcgcgtagtc gctcgggttc cattcggacg tggcgttcag tgcatcgagg
     1621 tacgaggcgt cctggcggtg agccgcccgc gccctctcgg gctcgcgata gatgagcgcg
     1681 caggagtcgt acggcgcgaa cagccacttg tgcgggtcga cgatgaacga atcgacgtcc
     1741 tcgattccgc ggaaccggtc tcggacgctc ggcgcgagga gccccgcacc cccgtacgcg
     1801 ccgtcgacgt gcagccagag ccctcgttcg cggcagacgg ccgcgacgcc ctcgaggtcg
     1861 tcgaccacgc ccgcgttcgt cgagccggcg ctcgcgacca ccgcgaacgc agcgccgggg
     1921 tcggtgcggt cgagcgccga tgcgagcgcg gggccggtca ggcggccgcg ttcgtcgtgt
     1981 ggggcggaga gcaggtcgac gtccatcacc cgcgccgccg acgcgaccga cgaatgcacc
     2041 gtgtcggcgc aggcgatctg ccagcgcggc gggcgttccc cgccccgagc gtcgctcgcg
     2101 gcgtggcgcg ccgcgaccag ggccgacagg ttccccgccg tcccccccga gacgaagcag
     2161 ccgccggagc cccctggcat cccggcgagc tcggcgagcc atcccagcac ctggttctcc
     2221 gcccatacgg cgccgcctcc ctcgagccag ctcccgccgt agacctcgga cgcgctcacg
     2281 gccaggtcga acaggacgct cgccttgctc ggcgcgccgg gcacgaacgc gaggaagctc
     2341 gggtggtcgg tcgtgatggt tgccggggcg agcacctcct cccagatccg cagggcttcc
     2401 cggccgccga tcccgtccgg cgtgatcgtg ggtccggccg accgcgtgag gtcttccgga
     2461 ggcacgggat ggtcgagcgg ctgtggatgc gcgatgcgcc ggcgcgtgta gtcgacgacg
     2521 gcccccgtca gcgtgtcggt ctccgaggtg tactcatgca tcgactgcat cggcgctccg
     2581 atctcgcggc ccctgccagg tgtcggggcg gctaggccac cagctcggcg cagaacgccg
     2641 cgtacgcgtc cacgtagcgc cggatgtcct cctcggtgtg ctgcacggag atcgtccact
     2701 gctcctcgtc gccgggcgtc atgaagatgc ctcggttcac catccacgtg aacgacgcat
     2761 agaagagctc ggggatcgtc tcgaggaagt cgcggtagtt g
//
LOCUS       DATBRK010000219         9482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_754938, whole genome shotgun sequence.
ACCESSION   DATBRK010000219 DATBRK010000000
VERSION     DATBRK010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754938"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..512)
                     /locus_tag="VIH70_06580"
     CDS             complement(111..512)
                     /locus_tag="VIH70_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116061.1"
                     /translation="MGNVEDRERPSDQDSDEWDDGSGEALEETIEHADRAAGAESFGT
                     TADEQERGEPFDERLAEERPDRPILDTELGLIDEDGPDVESELVGEAVGERDPFVAPE
                     DSAMSVRDEAPGAVDHPDDHSERDDADPNAE"
     gene            complement(609..1286)
                     /locus_tag="VIH70_06585"
     CDS             complement(609..1286)
                     /locus_tag="VIH70_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8116062.1"
                     /translation="MATFGLIHGAGDVGWYWHLVEAELRERGHDVVAPDLPCDDQSAG
                     LTEYADTVVEAIGARTDLVVAAQSLGAFTAPLVCERVAVDLLVLVAPMIPVPGEPPAD
                     YWTDTRYVEEVRERYDDEIALFYQDVPPELASAALKHGRAQSEARMHEPLPLEAWPDV
                     PTRVLLCRDDRLFPAPFLRRVAYERLGIIPDEIEGGHTPALSRPKELADRLEAYVAQA
                     GIAPGVR"
     gene            complement(1323..2300)
                     /locus_tag="VIH70_06590"
     CDS             complement(1323..2300)
                     /locus_tag="VIH70_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006138623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HEY8116063.1"
                     /translation="MSSILRPKAVGSGDVVAVAALSGGLEEGEASLFERGVEAIEQMG
                     FEVRVSPLVDPDRRWWWAAASPPVVAEEFNRLLRDPEVRAIFALTGGRLALSYLDLID
                     LDAVRADPKPLLGFSDISTLHLALHARTGLVSLHSDCVTHGFGYWHESDDGRRKELED
                     VYVRVLTGDGAPGVLPPGRPWECWRSGRAQGPLVGGMLNRLVRVQSTSYALTPEHFDG
                     AVLFWEEAFTSTSVVWSDLQVLRHAGVLDRIAGMVVGAPFEIEPTEGGPDTLREIVLD
                     VLGERDIPVLGNVDVGHDPPNLPMPLGVSAEMDADALTLSLPEPAVDAG"
     gene            2424..2957
                     /locus_tag="VIH70_06595"
     CDS             2424..2957
                     /locus_tag="VIH70_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8116064.1"
                     /translation="MATAAPQLQTFYDGWGNYHRLLVAAVRDLTAEQLRLRPAPGLWA
                     VWQLAGHMAGSRTAWFHDVLGEGDPALRDLFRVTSTTVPGLAIEDAGWEDDEDHPRGP
                     AEIVEGLERSWAMMDDCLQRWTPDDLAVEFSRLRRTGTQTFTRQWVVWHLIEHDLHHG
                     GEISLILGSHGLTGLDL"
     gene            2991..3443
                     /locus_tag="VIH70_06600"
     CDS             2991..3443
                     /locus_tag="VIH70_06600"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8116065.1"
                     /translation="MAQTGSDRDLMAEEEQLWNELHGLIDSLPRDKVEEPGYFAEGWT
                     AKDLIAHIGSWLAEAGAVLEQIRFGTYRPDEIDIDAMNATFFDSLHDTAYPDVRAQAI
                     TARNRMLRAWGSLPEHSPEADRWIDKAGPEHYAEHLPRLREWVQQLGP"
     gene            complement(3472..4533)
                     /locus_tag="VIH70_06605"
     CDS             complement(3472..4533)
                     /locus_tag="VIH70_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116066.1"
                     /translation="MQWIALPAGIVILLWTLLDVFRTLVMPRAARGRSRLSRILFRPM
                     WRPWRWVGVRRKTIQARERVLAGAAPFFFFVLLVGWVSLALLGYALILWSPAFVDGIE
                     RGGDSFLDALYVAGSSLFTLGVGADVTGSTRAVVVAAGATGLGLFAVVIAYLPVLYQA
                     FNRREVGVLLLDARAGSPPSGPELLHRMGSAGVASSLPELFAEWERWVADVLETHMSY
                     PLLALFRSPHDNTSWITSLGSVLDAATLIITSVDDEPDERAKLLYGTAVHAVEDLFYY
                     LRLTEREAVIQRDEFEHVLDDMKEDGFVVRPIDEAYERFTQKRAKYAPRLDAIAVLLA
                     APPGLWIGDRSFLGARTSH"
     gene            complement(4588..5013)
                     /locus_tag="VIH70_06610"
     CDS             complement(4588..5013)
                     /locus_tag="VIH70_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116067.1"
                     /translation="MPGGESSDTVESPGASTDPSGQGSEPPKLASFFRRRMLLGVLGV
                     VLVVGAAAVASRSDAEVTVPRLIGTRVDPQLDALRLRLEAADLALGDVTVAACPEYDI
                     PGASLEQPPGTIIEQHPSGGSSVVTSTAVDVTVCLPETG"
     gene            complement(5113..5931)
                     /locus_tag="VIH70_06615"
     CDS             complement(5113..5931)
                     /locus_tag="VIH70_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY8116068.1"
                     /translation="MSTWQTVALGAVAGFTIFLGLPLGRLRGGSLGLKTFLSGLSAGI
                     LVFLLFDVLAHATEPLEEAVAERAWGELVAVGLVYVAGLGVGLLSLPYFFRLILHRAR
                     GASLGPGAMAVAEIGLTPDRQALRLGMTIAVGIGLHNFSEGLAIGQAANANEISLAVL
                     LVVGFALHNATEGFGIVGPLAGAGVQATWRWLLVAGLVAGGPTFLGTIVGTSFSSEYV
                     FVGFLALAAGAILYVIGELFASGRRLSWILTLWGVLGGFLLGLATDLVIEAAGG"
     gene            6051..6815
                     /locus_tag="VIH70_06620"
     CDS             6051..6815
                     /locus_tag="VIH70_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEY8116069.1"
                     /translation="MARPAARAPKPSAPLERAVRSETVKHYAETIFYIRQEEGRVRPG
                     RLAEWLGVSAPTVTVTLQRLARDGFVEIGTDRSVTLTPTGEDLAAKIVRVHRLLERWL
                     TDVLGLDWAAADEEAQRLASGVSELVADRLDETLGHPATCPHGNVVPGREPPYGELVA
                     LSDLQPGVPARVRRISEVAEHDAPDLLRELDAYGLVTGARIEVSGADASVQAIPVDVG
                     GVTRPIGTNVGRLIWVERESSRRAATAKPRRATSGR"
     gene            complement(6820..8670)
                     /locus_tag="VIH70_06625"
     CDS             complement(6820..8670)
                     /locus_tag="VIH70_06625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8116070.1"
                     /translation="MDTSPRTPTSVRVPIAWKFFALLALIIPSLLAVSWVGGRGMVGM
                     KERLDTVYDDNLMSTQAIGSLSVALEEAEEMSLRLVNEVDRATIVGLDSELRQEVFPE
                     VARRLNDVRVLSGPDSSEDGVVARSLEAGWGEFVAFANSRAFLAATRGPSDDDRTVSS
                     TVERLSTSLRILTQQLEAQETRQAQVAMVEAQRAHAHSLSVLRTIVVLGLLAGVGATL
                     WLIRSVVVRTKEYSSFATRVAAGELNTRARPRGHDELTDLGWALNEMVARGESARGYE
                     LSQGEFTDALQVSESEDESYGLLKRHLERSIPRSAVVVLNRNNSADRLEATTEVPSDS
                     GLAERLATAQPRDCLAVRSAQRHDEEPGAEPLISCDLCHHIDGRTSCNPLLVGGEVIG
                     SVLVAHGDPVSDEHRLRVKDSVTQAAPVLANLRNLSLAQLRAATDSLTGLPNARAVQD
                     TLKLLVAQASRMLWPLGAVLLDLDHFKDINDTFGHGIGDEVLAAVGVALQSTVRESDF
                     VGRYGGEEFIMLLPDADRETTLQVAERVRTAIADINVLEGDSAVTASFGVAVFPEDAP
                     DAARLVRNADRALYRAKANGRNRVEMFTLDAATEDEPGPGTIASATPASS"
     gene            8816..9253
                     /locus_tag="VIH70_06630"
     CDS             8816..9253
                     /locus_tag="VIH70_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116071.1"
                     /translation="MDPRSETALRAEREAQFIANIGAFMRRDFDAIERSMRPDVVMEL
                     PGSSLLSGTYGGCAEVGRCVVALRRVLESDHTRISFLHEGDRMIVRHDIKVHGPNHDV
                     EMTLRVRARFDEQGKMESITVEPDDLRLFDHVLNAVLRDSEAS"
BASE COUNT         1524 a         3388 c         3249 g         1321 t
ORIGIN      
        1 ctcgcgaccc acctgcgata ccgggtgatg ccggacccgc agccgagtta gccagcggcg
       61 gtcggcacgg acgcacccgt ttgcaccgtc gctcggttgc gtgcgttggc ctactcggcg
      121 ttcgggtccg cgtcgtccct ctcgctgtgg tcatcgggat ggtcgacggc ccccggagcc
      181 tcgtcacgca ccgacatcgc cgagtcctcg ggcgcgacga acgggtcccg ttcaccgacc
      241 gcctcgccga ccagctccga ctcgacgtcc ggcccgtcct cgtcgatgag cccaagttcg
      301 gtgtcgagga tcggtcgatc cggccgctcc tccgcgagcc gctcgtcgaa cggttcgccc
      361 cgttcctgct cgtcggccgt ggtgccgaac gactcggcgc cggccgcgcg atccgcgtgc
      421 tcgatcgtct cctcgagggc ctccccggaa ccgtcgtccc actcatccga gtcctggtcg
      481 gaaggccgct cgcgatcttc gacgttcccc atggccgagt cccctctcat catcggcgag
      541 acgctccacc tcactcgaca atgatgacgc agatagccgg aactgatcgg ccgacaccgt
      601 tgcttcgcct accgaacccc tggggcgatg ccggcctgag ccacgtacgc ctcgagacga
      661 tccgccagct ccttcgggcg actcagggcg ggagtgtgtc cgccctcgat ctcgtccggg
      721 attatgccga ggcgctcgta ggcgacgcgg cgaaggaagg gggccgggaa caagcgatcg
      781 tctcgacaga gcaggacccg cgtgggcacg tccggccacg cctcgagcgg caacggttcg
      841 tgcatccgag cctcggactg cgcccgccca tgcttcaggg ccgcggacgc cagctccggc
      901 gggacgtcct ggtagaacag ggcgatctcg tcgtcatagc gctctcgcac ctcctccacg
      961 tagcgggtgt cggtccagta gtcggcgggc ggctcgccgg gtacggggat catgggtgcg
     1021 accagaacca gcagatccac cgccacgcgc tcgcagacga gcggggccgt gaacgcgccg
     1081 agcgattggg cggccacgac cagatcggtc cgggcgccga tcgcctcgac cacggtgtcc
     1141 gcgtactccg tcaggccggc cgactgatcg tcacacggaa ggtcgggcgc cacgacgtcg
     1201 tgaccccgtt cgcgcagctc ggcctcgacc aggtgccagt accagccgac gtcgccggcg
     1261 ccgtgaatca agccgaacgt agccatctcg gcccccttcg cggacacgtg gatcgatgcc
     1321 gatcagcccg cgtcgaccgc gggttccggc agcgacagcg tcaaggcatc ggcatccatc
     1381 tcggcgctga cgccgagcgg catcgggagg ttcggcgggt cgtggccgac gtcgacgttc
     1441 ccgagcaccg ggatgtcgcg ctcgcccagc acgtccagga cgatctcgcg cagcgtgtcc
     1501 ggcccgccct cggtgggctc gatctcgaac ggggcaccga cgaccatgcc cgcgatccgg
     1561 tcgagtacgc ccgcatgccg cagaacctgc aggtcgctcc agacgaccga ggtcgacgtg
     1621 aaggcctcct cccagaacaa gaccgcgccg tcgaagtgct cgggggtcag cgcgtacgac
     1681 gtggattgga cccgcaccag gcgattgagc atcccgccca cgagcggacc ctgtgcacgg
     1741 cccgaacgcc agcactccca gggacggccc ggcggcagga cgcccggcgc gccgtcgccg
     1801 gtcagcacgc ggacgtagac atcctcgagc tccttgcgac ggccgtcgtc gctctcgtgc
     1861 cagtagccga acccgtgcgt gacacagtcg gagtgcaggg acaccaggcc ggtgcgcgcg
     1921 tgcagcgcca gatgcagcgt cgagatatcg ctgaagccca gcagcggctt gggatcggcc
     1981 cgtacggcat cgaggtcgat caggtcgaga tagctgagcg ccaacctgcc gccggtgagc
     2041 gcgaagatcg ctcgcacctc cggatcgcgc agcagacggt tgaactcctc ggctaccact
     2101 gggggtgacg ccgccgccca ccaccaccgc cggtccgggt cgacgagtgg cgacacccgg
     2161 acctcgaacc ccatctgctc gatcgcctcg acgccgcgct cgaacagcga cgcctcaccc
     2221 tcctccagcc cgccggacag ggcggccacc gcaacgacgt cgcccgaccc aaccgccttc
     2281 ggacgcaaga tcgatgacat gtgagcctcc gttctcggga accggcttcc gacgatgctc
     2341 gcccggatcg aggacgacag tctagggacg agggcggtga cggcgccggg ccgcgcttca
     2401 tcgcggctgc ataggatgca cggatggcaa ccgcggcgcc ccaactgcag accttctacg
     2461 acgggtgggg gaactaccac cgcctgctcg tggccgcggt ccgagatctg accgccgaac
     2521 agctccgcct gcgccccgcg cccgggctct gggccgtctg gcagctggcc ggccacatgg
     2581 cgggatctcg gaccgcgtgg ttccacgacg tcctgggtga gggcgatccc gcgctccgcg
     2641 acctgttccg agtgacgagc acgaccgtcc ccggtctggc gatcgaggac gcgggatggg
     2701 aggacgacga ggaccacccg cgcgggccgg ccgagatcgt tgagggcctc gaacgctcgt
     2761 gggccatgat ggacgactgt ctccagcgat ggacgccgga cgatctggcc gtcgagttct
     2821 cccgcctgcg acgtacgggg acccagacgt tcacccggca gtgggtcgtc tggcacctga
     2881 tcgagcacga cctccaccac ggtggcgaga tctcactgat cctcggcagt cacggcctca
     2941 ccggcctcga cctctgaccg aggccgccgc gtgagcgtca tactcccccc atggcgcaga
     3001 cgggttcgga ccgggacctg atggccgagg aggagcaact gtggaacgag ctccacgggc
     3061 tcatcgactc gctgccccga gacaaggtgg aggagcccgg gtacttcgcc gaggggtgga
     3121 cggcgaagga tctcatcgcc cacatcggga gctggctcgc cgaggcgggc gccgtgctgg
     3181 agcagatcag gttcgggacc taccggccgg atgagatcga catcgacgcg atgaacgcga
     3241 ccttcttcga ctcgttgcac gacaccgctt atccggacgt gcgcgcgcag gcgatcaccg
     3301 cccgtaaccg catgcttcgc gcgtggggct cgctccccga gcactcaccc gaggccgacc
     3361 gctggatcga caaggccggc ccagagcact acgcggagca cctcccgcgc ctgcgggaat
     3421 gggtgcaaca gctcggcccc taggggtcgt tctcgtaggg ccgcaaacac gtcagtgcga
     3481 ggtgcgcgcc cccaggaagg agcggtcgcc gatccacaga cccggcggcg cggccagcaa
     3541 cacggcgatc gcgtccaacc gcggcgcgta cttcgctcgc ttctgcgtga atcgttcgta
     3601 ggcctcgtcg atgggacgga ccacgaaccc gtcctccttc atgtcgtcca ggacatgctc
     3661 gaactcatct cgctggatca ccgcctcccg ctccgtgagc cgcaggtagt agaagagatc
     3721 ctccaccgcg tggacggccg tcccgtagag gagcttcgcg cgctcgtcgg gctcgtcgtc
     3781 cacggaggtg atgatgagcg tcgccgcgtc gagcaccgag ccgagcgacg tgatccacga
     3841 ggtgttgtcg tgcggcgacc ggaacagggc gagcaggggg taggacatgt gcgtctcgag
     3901 cacgtcggcc acccatcgct cccactccgc gaagagctcc ggcagcgagg atgcgacgcc
     3961 ggcgctgccc atccggtgga ggagctcggg acccgacgga ggggatccgg cccgcgcgtc
     4021 gagcagcagc acgccgacct ctcggcggtt gaaggcctga tacagcaccg gcagatacgc
     4081 gatcaccacg gcgaacagcc cgaggccggt cgcccctgcc gcgaccacga cggctcgcgt
     4141 cgatccggtg acgtcggcgc cgaccccgag cgtgaacaac gacgatcccg cgacgtacag
     4201 agcgtccagg aacgagtcac cgccccgttc gatcccgtcc acgaaggcgg gactccacag
     4261 gatcagggcg tagcccagca gggcgaggga cacccagccg accagcagca cgaagaagaa
     4321 gaagggcgcc gccccggcca ggacccgctc gcgggcctgg atcgtcttgc ggcgcacgcc
     4381 gacccatctc cacggacgcc acatcggccg gaacaggatc cggctcaggc gggaccggcc
     4441 acgagccgcg cgcggcatca cgagagtccg gaacacgtcg agcaacgtcc acaggaggat
     4501 cacgatgccc gcggggagcg cgatccactg catcgccgca gcctaccgtg gcaccccctg
     4561 gcgttcctcc ggcgcaatcg gccgatctca gcccgtctcg ggcaggcaca cagtcacgtc
     4621 gacggcggtg gacgtgacga cgctcgatcc tccgctcggg tgctgctcga tgatcgtgcc
     4681 ggggggctgc tcgagggagg ctccggggat gtcgtactcg gggcacgcgg ccacggtgac
     4741 gtcgccgagc gcgagatcgg cggcctcgag ccggagccgc aacgcatcga gctggggatc
     4801 gaccctcgtg ccgatgagcc ggggaacggt cacctcggcg tcggacctcg acgcgacagc
     4861 ggcggcaccg acgacgagga ccacgccgag cacaccgagc agcatccggc ggcggaagaa
     4921 cgacgcgagc ttcggtggct cgctgccttg gccggacgga tccgtgctcg cgcctgggct
     4981 ctcgacggta tccgagcttt caccgcccgg catgcgcggt cccccgaacg acgaccacga
     5041 tcggaacgcg atcggccacc tcccgcaccc tctcgcgatg gaccacgggg gtcaaccgtg
     5101 gagatcttcg gtctagcctc cggccgcctc gatcacgagg tcggtcgcca ggccgagcag
     5161 gaacccgccg agcacccccc acagcgtgag gatccacgac aggcgccggc cgctcgcgaa
     5221 caactcgccg atcacgtaca ggatcgcccc cgcggcgagc gccaggaagc cgacgaacac
     5281 gtactcggag ctgaacgacg ttccgacgat cgtcccgagg aaggtcggac cgccggcgac
     5341 gaggccggcg accaggagcc accgccacgt cgcctggacc cccgcgccgg cgaggggccc
     5401 gacgatcccg aacccctcgg tggcgttgtg gagcgcgaag ccgaccacga gcaggaccgc
     5461 gagcgagatc tcgttcgcgt ttgccgcctg cccgatcgcg agaccctcgg agaagttgtg
     5521 caggccgatc ccgaccgcga tcgtcatgcc gagtcggagc gcctggcgat ccggcgtcag
     5581 gccgatctcg gcgaccgcca tcgcgccggg cccgaggctt gccccgcgcg ctcggtggag
     5641 gatgagccgg aagaagtacg ggaggctgag gaggccgacg ccgaggcccg cgacgtagac
     5701 gagaccgacg gcgaccagct cgccccaggc cctctcggcc accgcttcct cgagcggttc
     5761 ggtcgcgtgc gcgagcacgt cgaacaggag gaagaccagg atgcccgccg acagaccgct
     5821 gaggaaggtc ttgagcccga ggctcccgcc cctcagacgg ccgagcggga gcccgaggaa
     5881 gatcgtgaac cccgccaccg cccccagggc gaccgtctgc cacgtgctca tcccgtaccc
     5941 tccgagtttc gccgctcgaa tccgaccttc gcgatgggga actctaggct tagacgcgtc
     6001 gaagcgtcaa ggcacggcgg cgcgaacgcc ggcccggcta cgattcgcgc atggctcgcc
     6061 cggccgcgcg cgcacccaag ccctccgccc cgctggagcg cgcggtccgc tcggagacgg
     6121 tcaagcatta cgccgagacg atcttctaca tccggcagga ggagggacgg gtccgaccgg
     6181 ggcgcctcgc cgagtggctg ggggtgagcg cgcccacggt cacggtgacg ctgcagcggc
     6241 tggcccgcga cggcttcgtc gagatcggaa ccgaccggag cgtcaccctc acgccgacgg
     6301 gcgaggacct ggccgccaag atcgtccgcg tccaccggct gctcgaacgg tggctgaccg
     6361 acgtcctggg gctcgattgg gcggccgccg acgaggaggc ccagcgtctg gcctccgggg
     6421 tctcggagct ggtggcggac cggctcgacg agacgctcgg gcaccccgcc acgtgcccgc
     6481 acggcaacgt cgtcccgggc cgcgagcccc cgtacggaga gctcgtcgcg ctctcggacc
     6541 tgcagccggg ggtgccggca cgcgtgcgcc gcatctcgga ggtcgcagag cacgacgccc
     6601 ccgacctgct ccgggagctc gacgcgtacg ggctggtcac cggcgcccgg atcgaggtaa
     6661 gcggggcaga cgcgagcgtg caggcgatcc cggtcgacgt cggcggcgtg accagaccga
     6721 tcggcacgaa cgtcggccgg ctcatctggg tcgagcggga gtcttccagg agggcagcga
     6781 ccgcgaagcc gcggcgagcg acgtccggcc gatgacggcc tacgaggacg cgggtgtcgc
     6841 cgaagcgatc gtccccgggc ccggctcatc ctcggtggcg gcgtcgagcg tgaacatctc
     6901 gacgcggttc cggccgttcg ccttggcccg gtacagcgct cggtcggcgt tcctgacgag
     6961 ccgagccgcg tcgggggcgt cctccgggaa gacggcgacg ccgaaactcg cggtgacggc
     7021 cgagtcgccc tcgaggacgt tgatgtccgc gatcgccgtg cggacgcgct cggccacctg
     7081 gagggtcgtt tcgcggtcgg cgtccggcag cagcatgatg aactcctcgc cgccgtagcg
     7141 accgacgaag tcgctctcgc gcaccgtcga ctgcagggcg acgcccacgg ccgccaggac
     7201 ctcgtcaccg atcccgtggc cgaacgtgtc gttgatgtcc ttgaagtggt cgaggtccag
     7261 cagcaccgcg ccgagcggcc agagcatccg ggacgcctgg gccaccagca gcttgagcgt
     7321 gtcctggacc gcgcgtgcgt tcggcaaccc cgtcagggag tcggtggccg cgcggagctg
     7381 cgcgagggag aggttgcgga ggttcgcgag gacgggcgct gcctgcgtca ccgagtcctt
     7441 cacgcgcagg cgatgttcgt cgctgaccgg gtctccgtga gccaccagga ccgacccgat
     7501 cacctcccca cccaccagga gggggttgca cgacgtccga ccgtcgatgt ggtgacacag
     7561 gtcgcagctg atcagcggtt ccgcgccggg ctcctcgtcg tggcgctgag ccgatcggac
     7621 cgccaggcag tctcgcggct gcgccgtcgc caaccgttcc gcgagtccgg agtcggaagg
     7681 gacctcggtg gtcgcctcga gccgatccgc gctgttgttg cggttgagga ccacgaccgc
     7741 gctccgcggg atcgaccgct cgaggtgacg cttgagcagg ccgtaggact cgtcctccga
     7801 ctcgctgacc tgcagggcgt cggtgaactc cccctggctc agctcgtagc cacgggcact
     7861 ctcaccccgt gccaccatct cgttcagggc ccaaccgaga tccgtgagct cgtcgtgtcc
     7921 acgcgggcga gccctcgtgt tgagctcgcc cgccgcgacc cgagtcgcga agctcgaata
     7981 ctccttcgtc cgaacgacga ccgaacggat cagccacagc gtcgcgccca ccccggccag
     8041 cagcccgagc accacgatcg tccggagcac cgagaggctg tgggcatggg cgcgttgggc
     8101 ctcgaccatg gctacctgcg cctggcgcgt ctcctgagcc tccagctgct gggtcaggat
     8161 cctgagcgag gtgctcaacc gctccacggt cgaagagacc gtgcgatcgt cgtcgctcgg
     8221 ccccctggtg gcggcgagga acgcccgcga gttggcgaag gcgacgaact cgccccagcc
     8281 ggcctcgagg ctccgggcca cgacgccgtc ctccgaggag tccgggccgg acaggacgcg
     8341 gacgtcgttc aggcgccggg cgacctcggg gaacacctcc tgtcgcagct ccgagtcgag
     8401 gccgacgatg gtggcgcgat cgacctcatt gacgagacgc agcgacatct cctcggcctc
     8461 ctcgagtgcg accgagaggg acccgatcgc ctgtgtgctc atgaggttgt cgtcgtagac
     8521 ggtgtcgagt cgctccttca tccccaccat gcccctgccg cccacccagg agacggcgag
     8581 gagggaaggg atgatcagcg ccagcagcgc gaagaacttc catgcgatgg gcactcgcac
     8641 cgaggtgggc gttcgggggc tcgtgtccat ggtcgctcga tcgtccggct ggctcgggca
     8701 tgaggaagcc agtcctccga gtacctaaat cggcaccccc tcctcgggct tgagcacgcg
     8761 ctcgcgggat gccttccggc tcaaccgggc caggcccggt gccgatgcgt gtcgcatgga
     8821 ccctcggtcc gagaccgcgc tccgggcgga gcgtgaggct cagttcatcg cgaacatcgg
     8881 cgcgttcatg cgccgagact tcgacgccat cgagcggtcg atgcgaccgg acgtggtcat
     8941 ggagctgccg gggtcatcgt tgctctcggg cacctacggg ggctgtgcgg aggtcggccg
     9001 ttgcgtcgtc gccctgcgac gcgtgctgga atcggaccat acgcggatct cgttcctcca
     9061 cgagggtgat cggatgatcg tgcggcacga catcaaggtg cacggcccga atcacgacgt
     9121 cgagatgacg ctccgtgtgc gggcccggtt cgacgagcag ggcaagatgg agtcgatcac
     9181 ggtcgagccc gacgatctca ggctgttcga tcacgtcctg aacgcggtgc tccgcgactc
     9241 cgaggcctcc tagctcgagc gggccgggtg cgaggtgccc gaccggtcgc gcctggcatc
     9301 gactgcgggc tccaccccga ccgacccgaa gaggtccgcc gccacccgac cgcacgtgtt
     9361 ggatggtgcc cgacggggaa gaatcctcag atgcaggtcc agagccggcg atcggcgtcg
     9421 ggggccaggc agtgcctgga tctccagatg cccgcggtgt cggacgccct gacggacgtg
     9481 cg
//
LOCUS       DATBRK010000220        13077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_36231, whole genome shotgun sequence.
ACCESSION   DATBRK010000220 DATBRK010000000
VERSION     DATBRK010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13077
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36231"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1798
                     /locus_tag="VIH70_06635"
     CDS             101..1798
                     /locus_tag="VIH70_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116072.1"
                     /translation="MTRARLGALAVVVALIMPMVVGAMARAQQLPYERAALIIVVPGM
                     SFEELMSLPEAAALARAGGAGLLANEDDFTIPRVPGVPEGTPPGREHFLLLSRNRSAL
                     GQDIRDAVSSFPADELMVLLLSQGPSREMLAKGDELMPIVTAVGKPSDLFPDDGVEGS
                     LTSDSTLRRGVVTGEDIRATDVLFLNGPVDEFPGSPIEIIPGPPPFELHERYLAQRRM
                     YVPIGTAGAIYLAIAGLGAIVVLALGSRIPRGIRRIFGWASLSVAALATSLLAAGHLP
                     DLSYATVVPFVALVTVFGTLACAPLEDREVLLIPAGIGIAVLASFAVEAILSWTAALT
                     PFVGGSELDGGRFYGLPNVFIGLLIGACLWIAQRLRTLAGFALLVGVALFAGLPYLGA
                     NLGGGVSLFAAAGLWLAIRERERLGIWRGLGVFVGVTVLGTAVILAAHAISPFETHVT
                     RFEEQAGGLAGVVDRIGDRIQVGFDLIARNPFALIPVVGLPIAIAAVLRPPAALRPSL
                     QRSPAWRDAILVTLLAGVVAYLANDSGSAAAGFAFGLGLGGLLGVSLLSEAGKMGEP"
     gene            1795..2601
                     /locus_tag="VIH70_06640"
     CDS             1795..2601
                     /locus_tag="VIH70_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY8116073.1"
                     /translation="MSSQPPIPPRPDGLHPVPVIPARSDTLEVTDDARPKASWSWYEA
                     LVVYIVAFLVAGLATLPVLRLMEPEADLTNIVATIIAALVILGVLVLWLSTYHKGWLG
                     VMRFPERGTWGREIGSGVLFGLGLYPVMVFIVGGLLVYLLQTISGDPVKAPEQVGEHL
                     PAIGTALTIVYAIVIAPVGEELFFRGVLFRALRDRHGFWVGAAGSAVGFGLIHFIPGS
                     AIDAALLMIVMFFTGLALCFIYERRGTIVAPIAAHVTFNVIGIVLILGLR"
     gene            2611..3030
                     /locus_tag="VIH70_06645"
     CDS             2611..3030
                     /locus_tag="VIH70_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY8116074.1"
                     /translation="MALFPSDEWLTRYRELINGSEEYREAAEDWEGDVAFFLEAEPDR
                     GVPQDLVAWLDLWHGECRGARMITAAEGEGSAYGIRAPYSRWREVVLGDLEPVKAMVQ
                     GKLKLRGDLANIVRHVRAAKELVHLTTLVPTEFLGNA"
     gene            3034..4071
                     /locus_tag="VIH70_06650"
     CDS             3034..4071
                     /locus_tag="VIH70_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8116075.1"
                     /translation="MRAVVYRGVECVEVADVPDPVIEDPGDAIVRVGLTAICGSDLHL
                     YHGKAPLEPGDTIGHEAIGVIEQVGPAVTRFAPGQRVVVSFGIACGACWFCRKGQTQL
                     CEDFGFLGYGMFGGSLGGAQAELLRVPVADVNLLAVPEGLEDDRALFVGDALTTGVYA
                     AGLAGIADGDTVAVVGAGPVGFFCAQAALPSASSVLALDLDPARLALAERTGAIPVDV
                     ATRNAQTAVSEHTDGRGADVVIEAVGTPAAFERSLDIVRRGGTVVVMGVYTSELVQAQ
                     IGVWWIRALQLRFAGTTPVQTWWERAMAEVQAGRLDPLPIVSHRLPLEEAPRGYELFD
                     TKQATKVLLEP"
     gene            4094..4507
                     /locus_tag="VIH70_06655"
     CDS             4094..4507
                     /locus_tag="VIH70_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116076.1"
                     /translation="MTLRFADAEPNGLAELVGGLIETNLAAEPDRRALLRDTVVRLTA
                     SDAAIEATVTLSSAGVVVSNGPAGRRPHLVIAADAYDLIELAGAPLRFGFPDVLHPQG
                     RSVIRRIATRRVRVSGMLRHPIRLSRFTRLLSVVP"
     gene            4570..5085
                     /locus_tag="VIH70_06660"
     CDS             4570..5085
                     /locus_tag="VIH70_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116077.1"
                     /translation="MIALAAVLAAVTSGGYLTSDALGEIHGGAVAVGTSVRVTPLPGW
                     ELAGRSTDPDRIRLTSGSTSLDVAAFAFDGTDVDLLGRYVDDILKPDAEQFRVSEEIE
                     PVRLASGLTGSRIAYVGLFGDVQAPIEGEVTAVVSPSGEGVIFDGWAPAGQLQFEIDD
                     IDAMIERAEIA"
     gene            5085..6323
                     /locus_tag="VIH70_06665"
     CDS             5085..6323
                     /locus_tag="VIH70_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEY8116078.1"
                     /translation="MGGWRGGRLTIEPQVVRMPRTWGRQTSLFQWRQPAFWLYAAIVV
                     VAAVYTVSQQQLFQRLSPSGWALSWGLLLLYGFPLFLAIYLLDLYEREPMSLVLGALV
                     WGGVAATVLSGIANEGWGLVVARLGGPVFAAEWTAALTAPLVEETMKVAGVVLIYLIA
                     RDEMDDLMDGFVYGAMVGLGFTLVEDVFYFIAIFGGSPGGVLAGFYVRVVSSGLYGHV
                     LYTGVSGMGVAYFVSRRGEVAFGRRLAVALGLFVAAVAAHFLWNSPFLNLFPSSVDNV
                     GDWLRIPFAAAVKGAPLLLFVVVMVRLARRRERMWLETALRSEADSPWLSEVELRVLL
                     DPGARRRSRRQMAARAGDAAERLLKRLQKEQINLAMVRTRATSDDDVELVRQRALCAS
                     LRDALVAMPGAALAGTRREG"
     gene            6376..7308
                     /gene="meaB"
                     /locus_tag="VIH70_06670"
     CDS             6376..7308
                     /gene="meaB"
                     /locus_tag="VIH70_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017600330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY8116079.1"
                     /translation="MSLIEDVLAGDPRAIARAISMVEDGAAGLEALSAELFPRTGQAF
                     TVGLTGSPGVGKSTIVGELVGAARAQGRSVAVLAIDPTSPLSGGALLGDRLRMQAHAT
                     DPGVFIRSMATRGHLGGMALAAPEAIRVLDAAGHDLVIVETVGVGQAEVEVAAATDTT
                     VVVVSPGWGDAVQVAKAGILEIADVFVVNKSDREGSEVAVRDLQQMLRMGPERPWTPP
                     VVPTTATEGAGIDDLWAALESHRAHLASSGELERGRRDRLLHEVESLSLEKLRGRARD
                     LLTNDGSLAADLAERRTDPYRTAAILEERITHGP"
     gene            7368..9011
                     /locus_tag="VIH70_06675"
     CDS             7368..9011
                     /locus_tag="VIH70_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8116080.1"
                     /translation="MVEQRERRSSGDPRPSEARTLSGETLEPLYGPADLAGFDPEAKL
                     GRPGAYPFTRGIYPSMYRGRLWTMRQFAGFGSAAETNARYRFLLERGQGGLSVAFDMP
                     TLMGRDSDDPLAEGEVGRCGVATDTLEDFEALFDSIPLGDISTSMTISGPAVVVFAFF
                     LAAAERQGVPWEHLDGTLQTDILKEYIAQKEWIFPPRPHLRLIGDLMEFCLERVPRWH
                     PISVSGYHIREAGANAWQELAYTLADGFSYVELGLDRELDVDRFVPGLSFFFNAHVDF
                     FEEIAKYRAARRIWARWLRERYGAKDESSMRLRFHTQTAGVSLTAQQPMNNVVRTAIE
                     ALAAVLGGTQSLHTNALDEVLALPTEEAAKLALRTQQIIAHETGVPDVIDPLGGSYFL
                     ETLTDRMEELAEAEFARILAMGDGSMLEGVLTGIERGVFQQAIAESAFAEQERFERGD
                     LLKVGVTDFVDAGEQPIEILEISGGVETTQVERVREVRDRRDGAAARAALDRLGELAA
                     TNANLVEPLVDCARAMCTEGEIVEALRAVFGSYTETPRF"
     gene            9113..10003
                     /locus_tag="VIH70_06680"
     CDS             9113..10003
                     /locus_tag="VIH70_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116081.1"
                     /translation="MLAASEALGVGVVVSAFLFGFRHGIDWDHIAAITDITGSQEDRG
                     RALEYGTTYALGHALVVLVIGIAAIVLGAKLPDGVDAAMERVVGVTLIVLGVYVFSAL
                     VRHGREFRMRSRWMLIFGGVQGAFRKVRGGRGPEAHELEPVHRHAQDASLAVAVAEDI
                     PVSEWHHGHHGRPGHHHHKHPEPTEEAFMNYGRGTAFGVGMLHGIGAETPTQVVIFLT
                     AAGAGGPIAGMVILVVFLIGLLSSNTLITFGSAFGFVTASKNWTLYVTVAVLTASASL
                     IIGTLFLLGKGAVLPALFGG"
     gene            complement(10007..10462)
                     /locus_tag="VIH70_06685"
     CDS             complement(10007..10462)
                     /locus_tag="VIH70_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY8116082.1"
                     /translation="MTPTAAPASNADVISALRERGLRMTPQRRAIVAEIMRTQGHISP
                     TALARKVQGDMPGVNASTIYRTLALLEEMGVLAHAHLESGAEYHRAEEAGHVHLTCSN
                     CGDEDDLSIGEAAALIRLIEQHHGFEPDLTHFAIGGLCADCRKASGAAG"
     gene            complement(10512..11408)
                     /locus_tag="VIH70_06690"
     CDS             complement(10512..11408)
                     /locus_tag="VIH70_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8116083.1"
                     /translation="MEPTEREIANIRRALKRLRAEPTGWDPVRSGGHTPARRWIVMLD
                     DGRTAFAKVATDELTASWLRDEHVVYQHLLGASFVPGYVGYYDDGEHPVLALEDLSSG
                     TWPPPWDAGGISALLASLDEMWATEPPPGTALATDDAREIGGGWDEIARSPEPFLRLG
                     LCSEPWLSEHLDTLRSVADEAAFGGNALLHFDVRSDNVCFRPDDGRAVLIDWNLTSVG
                     NPQIDVVFWLPSLEAEGGPAPETVLPDADPRLVSTCAAFFCARAARPPIPTAPTVREV
                     QLVQARTALPWAARALGLPPPG"
     gene            11435..12199
                     /locus_tag="VIH70_06695"
     CDS             11435..12199
                     /locus_tag="VIH70_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018802578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8116084.1"
                     /translation="MGEFVRIERNGAVPTIRLDRPPANALAREVSLELSAAAKEVTAD
                     EGVRAVVVWGGERIFAAGADIKAMVDYGPEEVASDVGALEQACRDVESIPTLTIAAIN
                     GYALGGGCELALSCDFRFAARDARIGLPEVRLGIIPGSGGTQRLPRVIGLPKARDLVY
                     TGRRVDAAEALEIGLVDRVAEGDVHALAVEHATEFAKGPTLAYAAAKRALAASDPLEQ
                     GLAVEREAFVALFATRDQEEGMRAFLDKREPRFEGR"
     gene            12201..12692
                     /locus_tag="VIH70_06700"
     CDS             12201..12692
                     /locus_tag="VIH70_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8116085.1"
                     /translation="MRVRQATVEDVPSLVALFQELDRMQADWRVFTPRPGFYDEVGAK
                     YNEALADPDTVVLVAEDEDGEVVGMAYGEARTPSRFSDERALELSGVVVRAGYRGRGV
                     GRELVHEAGRFAADRGVRWVELKTFAPNRGAMEFWEDLGFTARVVQLTSPTKVLLERL
                     EGR"
     gene            12821..13030
                     /locus_tag="VIH70_06705"
     CDS             12821..13030
                     /locus_tag="VIH70_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116086.1"
                     /translation="MSDSAYRGARIRELPRANRTYPGGRVCAAEGCTTKLSIYNRWDL
                     CWQHEPVHTYISRGKRRSRKEMEAA"
BASE COUNT         1741 a         4461 c         4775 g         2100 t
ORIGIN      
        1 acatgtacca cgtcgaccgc ggctaccagg atctcgaggc caagctgacg gcgctcggcg
       61 ccgtcgtccg tcgcgagcgc gaacgctcac ccgctctcca gtgacccgcg cgcgtctcgg
      121 cgcgctcgcc gtcgtggtcg ctttgatcat gccgatggtc gtcggagcga tggctcgggc
      181 tcagcagctt ccgtacgagc gcgcagctct gatcatcgtc gtgcccggca tgtcgttcga
      241 ggagttgatg agtctccccg aggcggctgc gctggcccgg gcaggtggtg caggcctcct
      301 ggcgaacgag gacgacttca cgatcccccg cgtccctggg gttcctgagg gcacccctcc
      361 tgggcgcgaa cacttcctgc tcttgagtcg gaatcgatcc gcactcgggc aggacatccg
      421 agatgctgtt tcgtccttcc cggcggacga gctgatggtg ctcctattgt cgcagggccc
      481 atcccgggaa atgctcgcga agggcgacga actcatgccg atcgtcacgg ctgtcggcaa
      541 gccgagtgac ctcttcccag acgacggggt cgaaggctcg ttgacgtcag acagcacact
      601 acggcgtggc gtcgtcaccg gagaggatat tcgcgccacg gacgtcttgt tcctgaacgg
      661 gccggtggac gagttccccg gttcgcccat cgagatcatc ccgggacctc caccgttcga
      721 gctccacgag cgctacctcg cgcagcggcg catgtatgtc ccgatcggga cggcgggggc
      781 gatctacctc gcgatcgctg ggctcggcgc gatcgtcgtg ctagcgctcg ggagccggat
      841 tccccgaggc atccgacgga tcttcggatg ggcgtctctg tcggtcgccg cgctcgcgac
      901 ctcgttgctc gcggccgggc acctgcccga cctttcgtac gcaacggtcg tccccttcgt
      961 cgcgctcgtg accgtgttcg ggacgctggc gtgcgcgccg ctcgaagatc gagaggttct
     1021 gctcatcccc gcggggatcg ggatcgccgt cctcgcgtcc ttcgcggtcg aggcgatcct
     1081 gtcgtggacc gccgcgctca ccccgttcgt cggcggctcc gagctcgacg gcggccgctt
     1141 ctacggcctg ccgaacgtct tcatcggact cctgattggc gcgtgtctct ggatcgcaca
     1201 gcggctccgg accctcgccg gcttcgcgct cctggtgggg gtggcgctgt tcgccggcct
     1261 gccgtacctc ggcgcgaacc tgggcggcgg ggtctcgctg ttcgccgcgg cggggctgtg
     1321 gctcgcgatc cgcgaacgcg agcgtctggg catctggagg ggcctaggcg tgttcgtggg
     1381 cgtgaccgtg ctcggcaccg ccgtgatcct ggcggcgcac gcgatctcgc cgttcgagac
     1441 gcacgtgacg aggttcgagg agcaggcggg cgggctggcc ggcgtggtcg acaggatcgg
     1501 agaccggatc caggtcgggt tcgatctcat cgcccgaaac ccgttcgcac tgatccccgt
     1561 cgtcggcctg ccgatcgcga tcgcggcggt gctccgaccg ccggccgcgc ttcggccgag
     1621 cctgcagcgg agccccgcgt ggcgggacgc gatcctggtc acgctgctgg ccggcgtcgt
     1681 cgcctacctc gcgaacgact ccgggtccgc ggcggccggg ttcgcgttcg gcctggggct
     1741 gggggggctc ctcggcgtgt cgctgctgtc agaagccggg aagatggggg agccgtgagc
     1801 agtcagccac cgatccctcc ccgtcccgac ggcctccacc cggtcccggt gatccccgcg
     1861 cgttccgaca ccctcgaggt caccgacgac gcccgtccca aggcgtcctg gagctggtac
     1921 gaggcgctcg tcgtctacat cgtcgcgttc ctggtcgccg gcctcgcgac gctccccgtc
     1981 ctccggctca tggaaccgga ggccgacctc accaacatcg tcgccacgat catcgccgcg
     2041 ctcgtgatcc tcggcgtgtt ggtcctgtgg ctctcgacgt accacaaggg atggctcggg
     2101 gtgatgcgct tcccggaacg cggaacctgg ggcagggaga tcgggtcggg cgtcctgttc
     2161 ggactcggtc tgtatcccgt gatggtgttc atcgtgggcg gactcctcgt ctacctcctg
     2221 cagacgatca gcggcgatcc cgtgaaggcc ccggagcagg tgggtgagca tcttccggcg
     2281 atcgggaccg cgctgacgat cgtctacgcg atcgtgatcg cgccggtcgg cgaagagctg
     2341 ttcttccgag gcgtgctgtt ccgggcgctt cgcgatcgcc acgggttctg ggtgggggcg
     2401 gcgggctcgg cggtggggtt cgggctgatc cacttcatcc ccggttcggc gatcgacgcg
     2461 gccctgctca tgatcgtgat gttcttcacc gggcttgcgc tgtgcttcat ctacgagcgg
     2521 cgtggaacga tcgtcgcccc gatcgccgct cacgtgacgt tcaacgtgat cggcatcgta
     2581 ctgatcctcg gattgcggta aggaggaccg atggcgctgt tcccgagcga cgagtggctg
     2641 acgcggtacc gggagctgat caacggttcc gaggagtatc gcgaggcggc cgaggactgg
     2701 gagggcgatg tcgcgttctt cctggaggcg gagccggatc gcggcgtgcc acaggacctc
     2761 gtggcctggc tcgacctgtg gcatggcgag tgccgcggcg cgcggatgat cacggccgcg
     2821 gagggcgagg ggtcggcgta cgggatccgg gcgccgtact cgcgctggcg cgaggtggtg
     2881 ctcggcgatc tcgaacccgt caaggcgatg gtgcagggca agctcaagct gcggggcgac
     2941 ctggccaaca tcgtccggca cgtgcgcgcc gcgaaggagc tcgtgcacct cacgacgctc
     3001 gtgccgaccg agttcctcgg caacgcctga tcgatgcggg ccgtcgtcta ccgtggcgtc
     3061 gaatgcgtcg aggtcgctga cgtccccgat ccggtgatcg aggacccggg cgacgcgatc
     3121 gtacgcgtcg ggctcacggc gatctgcggg tccgacctgc acctgtacca cggcaaggcg
     3181 ccgctcgagc ccggcgacac gatcggccac gaggcgatcg gcgtgatcga acaggttggc
     3241 cccgcggtga cgcggttcgc gcccggtcag cgggtggtcg tctcgttcgg tatcgcgtgc
     3301 ggcgcgtgct ggttctgccg aaaggggcag acgcagctgt gcgaggattt cggcttcctt
     3361 ggctacggga tgttcggcgg gagcctgggt ggcgctcagg ccgagctgct ccgcgtcccc
     3421 gtggccgacg tcaacctgct cgccgtgccc gagggtctgg aggacgaccg ggcgctcttc
     3481 gtcggcgatg ccctgacgac cggcgtctac gcggccgggt tggccggcat cgccgacggc
     3541 gacacggtcg cggtcgtcgg ggccggaccg gtcgggttct tctgcgcaca ggcggcgctc
     3601 ccgagcgcgt cctcggtgct cgccctggac ctggaccccg ccaggctggc gctcgccgaa
     3661 cgcacggggg cgatcccggt cgacgtcgcg acgcgcaacg cgcagaccgc ggtgtccgag
     3721 cacacggacg ggcgcggcgc agacgtcgtg atcgaggccg tggggacgcc ggcggcgttc
     3781 gagcgctcgc tcgacatcgt gcgtcggggc ggcaccgtcg tggtcatggg cgtctacacg
     3841 agcgagctcg tccaggcgca gatcggcgtc tggtggatcc gcgcgctgca gctccggttc
     3901 gccggcacca cgccggtcca aacctggtgg gagcgcgcga tggctgaggt gcaggccggt
     3961 cggctcgacc ccctgccgat cgtctcgcac cggctgccgc tcgaggaggc cccgaggggc
     4021 tacgagctgt tcgacacgaa gcaggcgacg aaggtcctcc tggagccgtg aacgtgccgg
     4081 gctcggtcgc tccgtgacgc tgcggttcgc cgacgccgag ccgaacgggc tcgccgagct
     4141 cgtcggcggg ctgatcgaga ccaacctggc ggccgaaccc gatcgccgcg ccctcctccg
     4201 agacacggtc gtgcgcctga cggcctccga cgccgcgatc gaggcgacgg tcacgctctc
     4261 gagcgccggg gtcgtcgtct ccaacgggcc tgccggtcgc cggccgcacc tcgtcatcgc
     4321 ggccgacgcg tacgacctga tcgagctcgc gggcgccccg ttgcgattcg ggtttccgga
     4381 cgtcctgcat ccgcagggac gctcggtgat ccgtcggatc gcgacccggc gcgtgcgagt
     4441 atcgggcatg ctccgccacc cgatccgact ctcgcggttc acccggctgt tgtcggtggt
     4501 tccctgagcg ccggcgccga accggccccg ctgccggcgc cgccgcttcg tcagcgtcgc
     4561 tggttcccgg tgatcgcgct ggcggccgtg ctggccgcgg tgacgtccgg gggctacctg
     4621 acctccgacg cgctcggcga gatccatggc ggggccgtcg cggtgggcac gtcggtccgg
     4681 gtcaccccac tgcccggctg ggagctcgcc ggccgctcga ccgatccgga ccgcatccgc
     4741 ctcacgagcg gcagcaccag cctcgacgtc gccgccttcg cgttcgacgg gaccgacgtc
     4801 gacctgctcg gccggtacgt cgacgacatc ctgaagcccg acgccgagca gttccgggtc
     4861 tccgaggaga tcgaacccgt ccggctcgcc tccgggctca ccgggtcccg gatcgcgtac
     4921 gtgggtctgt tcggcgacgt gcaggcgccg atcgagggcg aggtgaccgc cgtggtgtcg
     4981 ccttccgggg agggcgtgat cttcgacggg tgggcgcccg ccgggcagct ccagttcgag
     5041 atcgacgaca tcgacgcgat gatcgaacgt gcggagatcg cctgatgggc gggtggcgag
     5101 ggggccggct cacgatcgag ccgcaggtcg tccggatgcc ccgtacctgg ggtcgccaga
     5161 cgtcgctgtt ccagtggcgg cagccggcct tctggttgta cgcggcgatc gtggtcgtcg
     5221 ccgccgtcta cacggtctcc cagcagcagc tgtttcagag gttgtcgccg tcggggtggg
     5281 cgctgtcttg gggcctcctc ctcctctacg ggttcccgct gttcctcgcg atctaccttc
     5341 tcgacctgta cgagcgcgaa ccgatgtcgc tcgtgctcgg cgccctcgtg tggggcggcg
     5401 tggcggccac ggtgctctcg ggcatcgcga acgaggggtg ggggctggtc gtcgcgcgcc
     5461 tggggggacc ggtgttcgcg gccgagtgga cggccgccct gacggcgccg ttggtcgagg
     5521 agacgatgaa ggtggccggc gtcgtgctga tctacctgat cgcgcgggac gagatggacg
     5581 acctgatgga cggcttcgtc tacggcgcga tggtcgggct cggcttcacc ctcgtcgagg
     5641 acgtcttcta cttcatcgcg atcttcggcg gctcgcccgg tggggtcctc gccggcttct
     5701 acgtgcgcgt ggtctcgagc ggcctctacg ggcacgtgct gtacacgggg gtgtccggca
     5761 tgggggtcgc gtacttcgtc tcgaggcgcg gcgaggtcgc gttcgggagg cggctggcgg
     5821 tcgcgctcgg gctgttcgtc gcggcggtgg cggcgcactt cctgtggaac tcgcccttcc
     5881 tgaacctctt cccgtcgagc gtggacaacg tcggcgattg gctccggatc ccgttcgctg
     5941 cggccgtcaa gggcgcgccg ctgctcctgt tcgtggtcgt gatggtccgg ctggcccgca
     6001 ggcgcgagcg catgtggctc gagacggcgc tgcggtcgga ggccgactcg ccctggctct
     6061 ccgaggtcga gctccgggtg ctcctggacc cgggcgcgcg gcggcggtcg cggcgccaga
     6121 tggctgctcg cgccggcgac gccgccgaac gcctgctgaa gcgcctgcag aaggagcaga
     6181 tcaacctcgc gatggtgcga acgcgcgcca cctccgacga cgatgtggag ctcgtgcgac
     6241 aacgggccct gtgcgcgtcg ctgcgcgacg cgctggtggc gatgccgggc gccgcgctgg
     6301 ccgggacgcg acgcgagggt tgatcccggc gccgtgcgtc accgtcgcgc cggggggccc
     6361 gggtaggctt cgagcgtgag cctgatcgag gacgtcctcg cgggcgaccc ccgagcgatc
     6421 gcccgggcga tctcgatggt cgaggacggc gccgccggcc tcgaggcgct ctcggcggag
     6481 ctgttccccc ggaccggaca agccttcacg gtcgggctca cgggctcgcc gggggtcggg
     6541 aagtccacga tcgtgggcga gctcgtcgga gcggcgaggg cacaagggcg ctcggtcgcg
     6601 gtgctggcga tcgacccgac atcgccactg tcgggcggcg cgcttctcgg cgatcgcctg
     6661 cggatgcagg cgcacgcgac ggatccgggc gtgttcatcc gctcgatggc gacgcgcggg
     6721 cacctcggtg ggatggccct cgccgcgccc gaagccatcc gggtgctcga cgcggcgggg
     6781 cacgacctgg tgatcgtcga gaccgtgggg gtcgggcagg ccgaggtcga ggtcgcggcg
     6841 gcgacggaca ccacggtggt cgtcgtctcg cccggctggg gcgacgcggt ccaggtcgcg
     6901 aaggccggga tcttggagat cgctgacgtc ttcgtcgtga acaagtccga tcgtgagggc
     6961 tccgaggtcg cggttcgcga cctccagcag atgctgcgga tgggtccaga gcggccgtgg
     7021 acgccccccg tagtgccgac caccgcgacc gagggcgccg gcatcgacga tctgtgggcc
     7081 gcgctcgagt cgcaccgtgc tcatctcgcg tcctccggcg agctcgaacg gggacggcgg
     7141 gaccggttgc tgcacgaggt cgagagcctc tcgctggaga agctccgggg ccgcgcgcgc
     7201 gacctcctca cgaacgacgg ctcgctcgcc gccgacctcg ccgagcggcg aacggacccc
     7261 taccggaccg cggctatcct ggaggaacgg atcacccacg gtccgtgacc gggaccggcc
     7321 acaggtcacg ccggcgcaac gagccgacgt ggcacccgag gaggaggatg gtcgagcagc
     7381 gcgaacgacg ttcctcgggg gaccctcggc cgtccgaggc tcggaccctc tcgggcgaga
     7441 cgctcgagcc cctctacggc cccgccgacc tcgcgggatt cgacccggag gccaagctgg
     7501 gacgcccggg cgcctacccg ttcacccgcg gtatctatcc ctcgatgtac cgcggccgcc
     7561 tgtggacgat gcggcagttc gccgggttcg gttcggccgc cgagaccaac gcgcgctacc
     7621 ggttcctcct cgaacgcggt cagggcggtc tgtcggtggc tttcgacatg ccgacgctga
     7681 tggggcgcga ctcggacgac cccctcgccg agggcgaggt cggacggtgc ggcgttgcga
     7741 ccgacacgct cgaggacttc gaggcgttgt tcgactcgat cccgctcggc gacatctcga
     7801 cgtcgatgac gatcagcggc cccgccgtgg tcgtgttcgc gttcttcctg gccgccgccg
     7861 agcgccaggg cgtcccgtgg gagcacctgg acggcaccct gcagaccgac atcctcaagg
     7921 agtacatcgc gcagaaggag tggatcttcc cgccgaggcc gcacctccgg ctgatcggcg
     7981 acctcatgga gttctgcctc gaacgcgtcc cgcgatggca ccccatcagc gtgtccgggt
     8041 atcacatccg cgaggcgggc gccaacgcct ggcaggagct cgcgtacacg ctggccgatg
     8101 ggttctcgta cgtggagctc ggcctggacc gcgagctcga cgtcgacagg ttcgtccctg
     8161 gcctgtcctt cttcttcaac gcgcacgtcg acttcttcga ggagatcgcg aagtaccgcg
     8221 cggcgcgcag gatctgggcg cgttggctga gggagcgcta cggcgcgaag gacgagtcct
     8281 caatgcggct gcggttccac acgcagaccg cgggggtctc gctcaccgcg cagcagccga
     8341 tgaacaacgt cgtccggacg gcgatcgagg ccctggccgc cgtgctgggt gggacgcagt
     8401 cgctccacac gaatgccctc gatgaggtcc tggcgctgcc gaccgaggag gccgccaagc
     8461 tcgcgcttcg cacccagcag atcatcgcgc acgagaccgg cgtgccggac gtgatcgacc
     8521 cccttggtgg ctcgtacttc ctcgagaccc tcaccgaccg gatggaggag ctcgcggagg
     8581 ccgagttcgc gcgcatcctc gcgatgggcg acggctcgat gctcgaaggg gtcctcaccg
     8641 gcatcgagcg aggcgtgttc caacaggcga tcgccgagtc agccttcgcc gagcaggagc
     8701 ggttcgagcg gggcgacctc ctcaaggtcg gggtgaccga cttcgtcgac gcgggggagc
     8761 agccgatcga gatcctcgag atctccggcg gcgtggagac cacgcaggtc gaacgcgttc
     8821 gcgaggtccg cgaccgacgc gacggggcgg ccgcgcgggc cgcgctcgac cggctgggtg
     8881 agctcgccgc cacgaacgca aacctcgtcg agccgctcgt cgactgcgct cgggcaatgt
     8941 gcaccgaggg cgagatcgtg gaggccctgc gggcggtgtt cggctcgtac accgagacgc
     9001 ctcggttctg agcccggcgt cgaggagcca gttgcaagag cacgcagttg ccagagggtg
     9061 cgtcaacgtg ctttcatgga cctgttccga ttcgggggaa ggatcgcagg gcatgctcgc
     9121 agcctcggag gccctcggcg tcggcgtcgt cgtgtcggcg ttcctgttcg ggttccggca
     9181 cggcatcgac tgggaccaca tcgcggcgat cacggacatc accggctccc aggaggaccg
     9241 gggccgggcg ctggagtacg gcacgacgta cgcgctcggc catgcgctgg tcgtgctcgt
     9301 gatcgggatc gcggcgatcg tcctgggggc caagctcccc gacggcgtgg acgccgccat
     9361 ggaacgcgtc gtcggcgtca cgctcatcgt gctcggcgtg tacgtcttct ccgcgctcgt
     9421 gcggcacggg cgcgagttcc ggatgcggag ccgatggatg ctgatcttcg gcggcgtcca
     9481 gggcgcgttc cggaaggtcc gtggggggcg cgggcccgag gcccacgagc tcgagccggt
     9541 gcatcgacac gcccaggatg catccctggc ggtcgccgtc gccgaggaca tcccggtgtc
     9601 ggaatggcac cacgggcacc acgggcggcc gggccaccac catcacaagc accccgagcc
     9661 gaccgaggag gccttcatga actacggccg ggggacggcg ttcggcgtcg ggatgctgca
     9721 cgggatcggc gccgagaccc cgacccaggt cgtgatcttc ctgaccgcag caggtgccgg
     9781 cggaccgatc gccgggatgg tgatcctggt cgtgttcctg atcgggttgc tctcgtcgaa
     9841 caccctgatc acgttcgggt ccgcgttcgg cttcgtgacg gcgtcgaaga actggaccct
     9901 ctacgtcacg gtcgcggtcc tgacggcctc cgcgagcctc atcatcggga ccctgttcct
     9961 gctcgggaag ggcgccgtcc tgcccgcgtt gttcggcggg tagggctcag cccgcggcgc
    10021 cgctcgcctt cctgcagtcg gcgcacagac cgccgatggc gaagtgcgtg agatcgggct
    10081 cgaacccgtg gtgctgctcg atcagccgga tcagcgcggc ggcctcgccg atcgacaggt
    10141 cgtcctcgtc cccgcagttc gagcaggtga ggtggacgtg gccggcctct tcggcccggt
    10201 ggtactcggc cccgctctcc aggtgggcgt gggcaagcac ccccatctcc tcgaggagtg
    10261 caagcgtccg gtagatcgtc gacgcgttca ctcccggcat atcaccctgg accttgcggg
    10321 ccagggcggt gggtgagata tggccctgcg tgcgcatgat ctcggcgacg atcgcccggc
    10381 gctgcggcgt catgcgcagc cctcgctcgc gaagcgccga tatcacgtcc gcgttcgatg
    10441 ccggcgccgc tgtcggtgtc acatcggcga ttgtagcagt gagttgcagg tgcaatgacg
    10501 ggatgtcgcg gtcacccggg cggcggcagg ccgagcgccc gagcggccca cggtaaggca
    10561 gtccgggcct ggaccaactg cacctcgcgg acggttggcg cggtcgggat cggcgggcgg
    10621 gccgcgcggg cgcagaagaa cgccgcgcat gtcgagacca gcctcgggtc ggcgtcgggc
    10681 agcacggtct cgggcgccgg gccaccctcg gcctcgagcg atgggagcca gaacacgacg
    10741 tcgatctgcg ggttccccac ggacgtgagg ttccagtcga tcaggacggc gcggccgtcg
    10801 tcgggccgga agcacacgtt gtcgctccga acgtcgaagt gcagcagcgc gttgcccccg
    10861 aacgccgcct catcggctac cgagcgaagg gtgtccaggt gctccgacaa ccacggctcg
    10921 gaacacagcc cgagacgcag gaagggttcg ggtgagcgag cgatctcgtc ccatccgccc
    10981 ccgatctcgc gcgcgtcgtc ggtggccagc gccgtccccg ggggcggctc ggtcgcccac
    11041 atctcatcca gcgacgcgag cagggccgag atcccccccg catcccacgg cgggggccag
    11101 gtccccgagg ataggtcctc gagcgccaac accgggtgct cgccgtcgtc gtagtagccg
    11161 acgtacccgg gcacgaacga ggctcccagc agatgctggt agacgacgtg ctcgtcgcgg
    11221 agccagctcg cggtgagctc gtccgtcgcc accttcgcga acgcggtccg gccgtcgtcg
    11281 agcatcacga tccagcgacg agccggcgtg tggccgccac tgcgcaccgg atcccacccg
    11341 gtgggctcgg cccgcaggcg cttaagggcc ctccggatgt tcgcgatctc gcgctcggtc
    11401 ggttccatag gcggtctgcg aggataggcc cgccatgggg gagttcgtac gcatcgagcg
    11461 gaacggggcg gtgcccacga tccgcctgga ccggccgcct gccaacgcgc tggcgcgtga
    11521 ggtctcgctc gagctgtcgg cggccgccaa ggaggtgacc gccgacgagg gcgttcgggc
    11581 ggtggtggtg tggggcggcg agcggatctt cgcggccggc gcggacatca aggcgatggt
    11641 ggactacggg ccggaggagg tcgcctccga cgtcggcgcg ctcgaacagg cctgccgcga
    11701 cgtcgagtcg atccccacac tcacgatcgc ggccatcaac ggctacgcgc tcggcggcgg
    11761 gtgcgagctg gccctgtcct gcgacttccg cttcgcggcc cgggacgcgc ggatcgggct
    11821 gcccgaggtc cggctcggga tcatccccgg ctcgggcgga acgcagcggc tgccccgggt
    11881 gatcggcctg ccgaaggccc gggatctcgt gtacacgggg cggcgtgtgg acgcggcgga
    11941 ggcgctcgag atcggcctgg tggaccgcgt cgccgaaggg gacgtccacg ccctggccgt
    12001 cgagcatgcg acggagttcg cgaagggccc gacgctcgcc tacgccgccg ccaagcgcgc
    12061 gctcgcggcg tccgatcccc tcgagcaggg cctggcggtc gagcgggagg cgttcgttgc
    12121 gctgttcgcg acccgggacc aggaagaggg catgcgcgcc ttcctcgaca agcgcgagcc
    12181 gaggttcgag gggaggtagc gtgcgcgttc gacaggccac ggtggaggac gtcccgtccc
    12241 tggtcgcgct gttccaggag ctcgatcgga tgcaggcgga ctggcgtgtc ttcaccccgc
    12301 gcccgggctt ctacgacgag gtcggtgcca agtacaacga ggcgctcgcc gaccccgaca
    12361 cggtggtcct ggtcgccgag gacgaggacg gcgaggtcgt cgggatggcg tacggtgagg
    12421 cccgtacccc gtcccgcttc tccgacgagc gagccctcga gctctcgggc gtggtcgttc
    12481 gcgccggcta ccggggccgg ggtgtgggcc gggagctggt ccacgaggcc ggccggttcg
    12541 cggccgatcg gggggtccgc tgggtcgagc tcaagacctt cgcccccaac cggggcgcca
    12601 tggagttctg ggaggacctc ggcttcaccg cacgggtcgt gcagctcacg agccccacga
    12661 aggtcctgct ggagcggctc gagggccgct gacaggcacg cccgctcgcc cgaaatcagg
    12721 ggtgctcggg aacgaaccgg cccgccgatt ggttcgaatg aacggattga agcctcgggt
    12781 cgaccggggc gaggttcgaa ccgaggcgag gagaggatcg atgtccgact cggcataccg
    12841 cggagcccgc atccgcgagc tccctcgggc caaccgtacg taccccggag gtcgggtctg
    12901 cgccgccgaa gggtgcacca cgaagctgtc catctacaac cggtgggatc tgtgctggca
    12961 gcacgagccc gtccacacgt acatctctcg cggcaagcgc aggagccgaa aggagatgga
    13021 ggccgcgtaa gccccccgta cgcgccttcc ctacggaagc cctcccgcgt tcgtggg
//
LOCUS       DATBRK010000221         7793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2199342, whole genome shotgun sequence.
ACCESSION   DATBRK010000221 DATBRK010000000
VERSION     DATBRK010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199342"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VIH70_06710"
     CDS             complement(<1..315)
                     /locus_tag="VIH70_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116087.1"
                     /translation="MRRTLALLGVLAVAATVIQAKASEDAQRLTRHRIERAGTGTTRF
                     VVAAAGDIACQSAPYGSGSPANCQYDDTARLIDGADLARVLPLGDNQYEVGAFAAYMS
                     YFD"
     gene            complement(397..585)
                     /locus_tag="VIH70_06715"
     CDS             complement(397..585)
                     /locus_tag="VIH70_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116088.1"
                     /translation="MPELQGLGWGARIMDAVMDYLDASVRPGSFIGLMAAKDVEGFYR
                     RYGLERRSDDRPGMFRIR"
     gene            829..1464
                     /locus_tag="VIH70_06720"
     CDS             829..1464
                     /locus_tag="VIH70_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116089.1"
                     /translation="MSEPVRFRGAGGVRLEGRVFGSGEVGVVLGHMRPGDQSQWLGFA
                     ALLADRGYVVLTYDRRGVCPGGELGCSGGQMSDSGWQDLAHAVDLLRERGASRVVVGG
                     ASLGAMESMYALSRGLDADGLIWLSGVDLYGGVPVTEQVRAVHVPKLLAAGEFDSDAV
                     ALRPAVERAAPPPTEVVLLDTGEHGTDILEFGDPAVADEFRRAVLDFLAGI"
     gene            1558..1893
                     /locus_tag="VIH70_06725"
     CDS             1558..1893
                     /locus_tag="VIH70_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003914096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116090.1"
                     /translation="MDRRTKWIAGGAIAVALVGGGAGVAIATGAGDTDRPLEGSTLDR
                     ARAAALESTGGGTVTETEAGDDGAAYGVEVRLDDGRVVEVRLDASFHVLGQEADDDGP
                     DDQDGSNED"
     gene            1924..3351
                     /locus_tag="VIH70_06730"
     CDS             1924..3351
                     /locus_tag="VIH70_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116091.1"
                     /translation="MNGHRGLVPRDRSNGVRRLVLIVAVALVTAACTSDAASPTGASG
                     SGSASGGAAGTGASGVTIPQPAVPASIPVAPESERVDLAMPTFSDPTNITNPLFPVSL
                     QDSVLMLGHVEGKPFRTEVTLLPETRIIEWEGQRVETLVSQYNAFLDGRIEEVAYDYY
                     AQADDGSVWYFGEDVFDFRAGAIVVTEGTWLAGRDGPAAMIMPADPQVGDVYRTENAP
                     GFVFEEVTVRSTDETLDGPLGPIQGGMLADELHSDGKTEQKVFAPGYGEFYTAGGGDV
                     EALALAVPTDAATEPLPPELTTMSSGALDVFDMAGSGDWAAASATVREMQAAWGTYRT
                     GDVPSLIEPRMDRALEVLARAVRSRQTAGSRNAAIQAARSSLDLQLRYRPQAEIDLAR
                     MDLWAAQLLVDEAAGDADGVGADQFALDYDRDRIRDAVDARDLVRINTEVGAIQVAVV
                     DGELAAAAAAAERLRVTLADLQPLG"
     gene            complement(3434..3673)
                     /locus_tag="VIH70_06735"
     CDS             complement(3434..3673)
                     /locus_tag="VIH70_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116092.1"
                     /translation="MQGTPWVVVEADDKQTIQREFAQGEIVDQFPSKQHSVFLVQTPK
                     VPSLPNTVLQGPIYGVGIFREERRARTLAKHLAES"
     gene            3888..4544
                     /locus_tag="VIH70_06740"
     CDS             3888..4544
                     /locus_tag="VIH70_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016870227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8116093.1"
                     /translation="MNLLVVQEEPPVASFIKRGLEAEGFVVRTACDGAEGLEMSRSSD
                     LDLIVLDLVLPTVTGEEILATLRAAGSSVPVIVLTASDAIPDRVAILNAGADDYLTRP
                     CSFSELLARIRARLRAADQTVSTTISYGRVTIDVATRDVRIGGRSVDLTPREFALLEA
                     LMRHAGQVLSQAQLLDQVWGYDRDPTSNVVEVYVGYLRKKLRRDVVETIRGAGYRFRG
                     "
     gene            4959..5423
                     /locus_tag="VIH70_06745"
     CDS             4959..5423
                     /locus_tag="VIH70_06745"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8116094.1"
                     /translation="MPEEAPLPPVRSRADRTADTLERLSRDVDLWVASASEDGRAYLV
                     PLSYHWDGSTLTIATPSASPTARNLIRAGWVRAALGQTRDVVIVEGRVEAIPIGADPE
                     LGDAHARATGFDPRTLADEYVYLRIAPREIQAWRESNELVGRVLMRDGAWLG"
     gene            complement(5460..5846)
                     /locus_tag="VIH70_06750"
     CDS             complement(5460..5846)
                     /locus_tag="VIH70_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116095.1"
                     /translation="MDKVVHFEIPVDDAARAREFYGSAFDWDLTEMDMGGGNMYTTVT
                     TTPIDETTRQPTEPGGINGGLMQRTGDTPAPVITIGVTSIDESLKKVEAGGGTTVQPR
                     TEIPEMGAFAYFRDSENNVVGLWENF"
     gene            complement(6205..6930)
                     /locus_tag="VIH70_06755"
     CDS             complement(6205..6930)
                     /locus_tag="VIH70_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8116096.1"
                     /translation="MPARSGLSAPKEPRNRLLALLPNGERERLSSSMERIALEPHDVI
                     QRPGGAFRHVYFPLWGVVSLMTPLEDGTAVETATVGNEGMVGIHAVLGGGTIGNAQAI
                     GQIPGEVLRMNADHFRAEIDGDGKLRQIVFAYMQALFVQISQGVACNGVHPIQQRCAR
                     WLLESHDRAESDEFLLTQEFLADMLGVRRASVTVAARTLQMAGMIEYERGHITVTDRA
                     ALEDASCECYAVIKQEYRRLVTA"
     gene            7190..7501
                     /locus_tag="VIH70_06760"
     CDS             7190..7501
                     /locus_tag="VIH70_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8116097.1"
                     /translation="MDFRVERDGDHEFRLVGELDMASASELFSQLERDAQQDGDLRLD
                     LSQLTFMDSTGVHVLARLADRCANGHRVVVEGPTARVAKLFELVGAESYPNVEIRNGR
                     S"
     gene            complement(7522..>7793)
                     /locus_tag="VIH70_06765"
     CDS             complement(7522..>7793)
                     /locus_tag="VIH70_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8116098.1"
                     /translation="LALLTSELVSNAVVHAGMAATQDIVVKVADGKCLRVEVLDKGSA
                     FDPPDQRIRSEPIGGWGLRLVDRLAARWGTDREDRGNKVWFELDR"
BASE COUNT         1181 a         2632 c         2724 g         1256 t
ORIGIN      
        1 gtcgaagtag ctcatgtagg cggcgaacgc gcccacctcg tactggttgt cgccgagcgg
       61 cagcacgcga gccaggtcgg cgccgtcgat caggcgagcg gtgtcgtcgt actgacagtt
      121 cgccggcgag cccgacccgt acggggcgct ctggcaggcg atgtcgcccg cggccgcgac
      181 gacgaatcgc gtcgtccccg tgcctgcgcg ctcgatgcgg tgacgggtca gccgctgtgc
      241 gtcctccgag gccttggcct ggatcaccgt ggccgcgacg gcgagcacac caaggagggc
      301 gagggtcctt cgcatggcgc ccatgctacc gagagcgtcg tcgccggaag ggagggctgc
      361 cgatcgctcg tgaccgaagg gctcgccgag cgagtttcaa cggatccgga acatccccgg
      421 gcgatcgtcc gagcgccgtt cgagcccgta ccgccggtag aagccctcga cgtccttcgc
      481 ggccatcagc ccgatgaacg agcccggtcg aaccgacgcg tccaggtagt ccatcacggc
      541 gtccatgatg cgcgcgcccc atcccagccc ctggagctcg ggcacgacga tgacgcacct
      601 cggcagtgta gagaggcacc ggcagggtcc tggccatcgc cgcgagaggg cgccttcgta
      661 tctctcggag agatgcgaac gcgatcgatc gtcgcggcgg catgcgtgac gcttgccctg
      721 tcgagctgcg cgggggagcg gtccggaccg ggcccatctc cgtcggcccc ccctccgccg
      781 gaggtcacgt ccgccttccc ggcgttcgat cgtaccgagg ccctctcgat gtcggaaccg
      841 gtccggttcc gcggagctgg tggggtgcgc ctggagggtc gcgtcttcgg ttcgggtgag
      901 gtcggcgtcg tgctgggaca catgcggccc ggcgaccaat cccagtggct cggcttcgcg
      961 gcgctgctcg ccgaccgcgg gtacgtggtc ctgacctacg accggcgggg cgtctgtccg
     1021 gggggcgagc tcggctgctc gggcgggcag atgagcgaca gcggctggca ggacctggcg
     1081 cacgcggtcg atctgctccg cgaacgaggg gcgagccgtg tcgtggtggg gggcgcgagc
     1141 ctcggggcga tggaatcgat gtacgcgctc tcccggggtc ttgatgccga cggcctcatc
     1201 tggctgtccg gggtcgatct gtacgggggc gtcccggtga ccgagcaggt gcgggccgtg
     1261 cacgttccga agctcctggc ggcgggcgag ttcgacagcg atgccgtcgc gctgcgtcca
     1321 gcggtcgagc gcgcggcccc gccgcccacc gaggtcgtcc tcctcgacac gggcgagcac
     1381 ggcaccgaca tcctcgagtt cggcgacccc gccgtggcgg acgagttccg gcgggcggtg
     1441 ttggacttcc tcgccgggat ctgagcgtcc cccaagcgct ctcatccgcc cttcacgctc
     1501 ctctcatcgc cgttgcgaca tcctggttcc cgaaagcaga ccgaggaggt cgatcgcatg
     1561 gatcgcagga cgaaatggat cgccggaggc gccatcgcgg tggcgctcgt cggcgggggt
     1621 gcgggcgtcg ccatcgccac cggagcggga gacacggacc ggcccctcga aggctcgacg
     1681 cttgaccggg cacgcgcggc cgcgctggag tccaccggcg gcgggaccgt gacggagacc
     1741 gaggcgggcg acgacggagc cgcgtacggg gtggaggtgc gcctggacga cgggcgggtg
     1801 gtcgaggtcc gcctggacgc aagcttccac gtgctcggcc aggaggccga cgacgacgga
     1861 cccgacgacc aggacgggtc gaacgaggac tgacccggtc cgatccagga cgacggagga
     1921 gccatgaacg gccatcgggg actcgtgccg cgcgatcgct cgaacggtgt gcgtcggctc
     1981 gtgctgatcg tcgcggtggc gctcgtgacc gccgcctgca ccagcgacgc ggcctcgccg
     2041 acgggcgcga gcggctcggg ttcggcgtcg ggcggagcgg cgggcacggg cgcgtcgggc
     2101 gtgacgatcc cgcagccggc cgtgccggct tccatcccgg tcgctccgga gagcgagcgg
     2161 gtcgacctcg cgatgccgac cttctcggat ccgacgaaca tcacgaatcc gctcttcccc
     2221 gtctccctgc aggactcggt cctcatgctc ggccacgtgg agggcaagcc gttccggacc
     2281 gaggtcacgc tcctgccgga gacccggatc atcgagtggg agggacaacg cgtggaaacg
     2341 ctggtgtccc agtacaacgc cttcctggac ggccgcatcg aggaggtcgc atacgactac
     2401 tacgctcagg ccgacgacgg ctccgtctgg tacttcggcg aggacgtgtt cgatttccgc
     2461 gccggggcga tcgtcgtgac cgaaggcacc tggctcgccg gcagggacgg cccggccgcg
     2521 atgatcatgc cggccgatcc ccaggtgggc gacgtctacc gaaccgagaa cgctccgggc
     2581 ttcgtgttcg aggaggtcac ggtccggtcc acggatgaga cgctcgacgg cccgctcggt
     2641 ccgatccagg gcgggatgct ggccgacgag ctgcacagcg acgggaagac cgagcagaag
     2701 gtcttcgctc ccggctacgg cgagttctac acggccggcg ggggcgacgt ggaggccctc
     2761 gcgctcgcgg ttcccacgga cgcagcgacc gagccgctcc cgcccgagct cacgacgatg
     2821 tcgagcgggg cgctcgacgt cttcgacatg gccgggtcgg gagactgggc ggcggcttcc
     2881 gcgacggtac gggagatgca ggcggcgtgg gggacctatc gaaccggcga cgtgccgagc
     2941 ctgatcgaac cccggatgga ccgggcgctc gaggtgctcg cgcgtgccgt ccggtctcga
     3001 cagacggccg ggtcccgcaa cgcggcgatc caggccgccc ggtcgagcct cgatctgcag
     3061 ctgcggtacc ggccccaggc cgagatcgac ctggcgcgca tggatctgtg ggccgcgcag
     3121 ctgctggtgg acgaggcggc tggcgacgcc gatggggtcg gcgcggacca gttcgcgctc
     3181 gattacgacc gcgaccggat ccgggacgcc gtcgatgccc gagacctggt tcggatcaac
     3241 accgaggtcg gcgcgatcca ggtcgccgtg gtcgacggcg agctcgccgc cgctgccgcc
     3301 gcggccgaac ggcttcgggt cacgctcgcc gacctgcaac ccctgggctg aacgcgcaga
     3361 cgaaagcgga cgggtcccga ccccgatcgg cccaggaccc gtccgctcgc ggtgcgtgct
     3421 cccgagggga gccctagctc tcagcgagat gcttggcgag cgtgcgcgcc ctccgctcct
     3481 cccggaagat gcctaccccg tagatggggc cctggagcac cgtgttcggc aacgacggca
     3541 ccttgggagt ctggacgagg aagaccgaat gttgctttga ggggaactga tccacgatct
     3601 cgccctgggc gaactctcgc tggatggtct gcttgtcgtc ggcttcgacg acgacccatg
     3661 gagtcccttg catggtcgca cctccttccc gcgtaccaac caggatgtct ggcggccctg
     3721 aagccaggct gagactgcgc tgagagatcc ctcatccgcc tcacggtcat acacggggtc
     3781 caggtgtttt gtcgcccgga gaccggccgt ccaacatggt ggcacgggga cggtccccct
     3841 ccccggggga tcacctggct cggaacgttc ggccggaggg ggtgctcgtg aacctgctgg
     3901 tcgtgcagga ggaacccccc gtcgcctcct tcatcaagcg gggactggag gccgagggtt
     3961 tcgtcgtccg gaccgcgtgc gacggcgccg aaggcctgga gatgtcgaga tcgtccgatc
     4021 tcgatctcat cgtcctggat ctcgtccttc cgaccgtgac cggtgaggag atccttgcca
     4081 cgttgcgcgc cgcgggttcg tcggtgcccg tgatcgtgct caccgcgagc gatgcgatcc
     4141 ccgaccgggt ggccatcctg aacgccgggg ccgacgacta cctgacgaga ccctgcagtt
     4201 tctcggagct gctcgcccgg atccgggcga ggctccgcgc ggccgatcag acggtgtcga
     4261 ccacgatctc ctacgggcgg gtcaccatcg acgtcgcgac acgggacgtc cggatcggag
     4321 ggagatcggt cgacctcacg ccccgggagt tcgccctgct cgaggcgctg atgcgacacg
     4381 cgggtcaggt cctctcacag gcccagctcc tcgaccaggt ctggggatac gaccgcgatc
     4441 cgacctccaa cgtggtggag gtctacgtgg gctatctccg caagaagctc cgacgcgatg
     4501 tcgtggagac gatccgcggc gccgggtatc ggttccgcgg ctgacgactc tggatccgtt
     4561 cgttcgctcg cctctccgga tcgaccctcg gagatcgtgg tgacctcgtt ggttgccttc
     4621 cgcggggttc tgccgcacca tgcgagtacg ggagaaagga ggtggtcctt gccgaaacag
     4681 ctcgagaccc tcgaggtgac tggctgctga gccgtccctc gcgggccggg gccgccagcc
     4741 ccgcccgttc gccgggatgc ccggcagaat ccaaccagct caccgacctc gcgggttccg
     4801 gttggtcgac cggacgacgc ctcggaccga ggccgaagcg ctcgcgggga ccgcgtcatc
     4861 ggacgggccg gcccatgtcg ggccggcccg tccgcgttgg atgacgcgtc gctcaggcgt
     4921 ccgggaccgt gaggtcgagc tccccgatgc gtccggagat gccggaggag gccccgctgc
     4981 cgccagtgcg atcgcgggcg gatcgcacgg ccgacacgct ggagaggctg agccgtgacg
     5041 tcgacctgtg ggtcgcctcg gcgagcgagg acgggcgcgc ctacctcgtc cccctctcgt
     5101 atcactggga cggctcgacg ctgacgatcg ccacgccgag cgcgagcccc accgccagga
     5161 acctgatccg ggccggctgg gttcgcgcgg cgctcgggca gacccgcgac gtcgtgatcg
     5221 tcgaagggag ggtcgaggcg atcccgatcg gcgccgaccc cgagctcggg gacgcgcacg
     5281 cacgagcgac ggggttcgat ccgcggacgc tcgcggacga gtacgtctac ctacggatcg
     5341 ccccgcggga gatccaggcg tggcgcgagt ccaacgagct cgtgggccgc gtgctcatgc
     5401 gcgacggagc gtggctcggg tagaccgacc actccgctcg atcggttcgg gtcaagaggt
     5461 cagaagttct cccacaaccc gaccacgttg ttctcgctgt ccctgaagta ggcgaaggcg
     5521 cccatctccg ggatctcggt gcgtggctgc accgtggtac cgcctcccgc ctccaccttc
     5581 ttcagtgact cgtcgatcga cgtgacgccg atcgtgatca ccggggcagg cgtgtcgccg
     5641 gtccgctgca tgagaccgcc gttgatgccg cccggctccg tcggctgccg cgtcgtctcg
     5701 tcgatggggg tggtcgtgac cgtcgtgtac atgttgccgc cacccatgtc catctcggtc
     5761 aggtcccaat cgaaggcgga gccgtagaac tcacgtgccc tggccgcgtc gtcgacgggg
     5821 atctcgaagt gcacgacctt gtccatgcct tcctccttcc cgaggcctcg agagcctctc
     5881 cgtgaccttt cctcaaccta tcgcgggttt gcccgctcgg accacacgaa ataccgactg
     5941 cgggcgagag agtcttggcc ggagcgattc gacgtatatc acgatcgtgt gagttggcat
     6001 tcgtacccct agcggaacgt ctgtagtctc ttcaccccac aaaaatcgac cgctcggagc
     6061 ccgctacgtg gggggaccgc tcagaagctc aggtaggtgc tcacctgggt cggtggacac
     6121 gatcgtcgct gggagccggg accacagtcc tgccctgcag ccggacctac cgccgtgcgc
     6181 cgccgccggc catcgtcacc gatgtcaagc ggtaacgagg cggcgatact cctgcttgat
     6241 cacggcgtag cactcgcacg atgcgtcctc gagcgccgcc cgatccgtca cggtgatgtg
     6301 cccgcgttcg tattcgatca tgccggccat ctgcagcgtt cgagcggcca cggtcacgga
     6361 ggctcgtcgc actcccaaca tgtcggccag gaactcctgc gtgagcagga attcgtcgga
     6421 ctcagcccgg tcgtggctct ctaacagcca ccgggcgcat cgttgctgga tcggatggac
     6481 gccgttgcag gcgaccccct gcgagatctg aacgaacagg gcctgcatgt acgcgaacac
     6541 gatctgccgg agtttgccgt ctccgtcgat ctcggcccga aagtggtcgg cgttcatccg
     6601 caggacctca ccggggatct gaccgatcgc ctgcgcgttg ccgatcgtgc ccccgcccag
     6661 cacggcatgg atccccacca tgccctcgtt gcccaccgtg gcggtctcga cggcggtgcc
     6721 gtcttccagc ggggtcatga gcgagacaac gccccaaaga gggaaataga cgtgccggaa
     6781 ggcccctcct ggcctctgga tgacgtcgtg gggctcgagg gcaatccgct ccatactcga
     6841 gctgagtcgc tcgcgctcgc cgttcgggag caacgccaag agccggttcc ttggctcctt
     6901 gggcgcagat agccctgagc gtgccggcat gccggccccc tttgccagtc gaactggggg
     6961 gagactttgg cgaggctcgg ggccaggaaa acgactgggg tttggagccc gctcctgagg
     7021 ccgggaacac cggaaagcat cccggttgcc gcttcactat acgctctgcc tggaaccgcg
     7081 acggtcgcga cggtccggac aaacgcggcg gatgtgcggt tccggacaga catgtcggcg
     7141 ggtccggaat aacctccgtg cgtctggcca agcgtggggg agagaagtcg tggacttccg
     7201 tgttgagcgg gacggcgacc acgagttccg gttggttggc gagctagata tggccagtgc
     7261 cagtgaactg ttcagtcagc tggaacgcga tgcgcaacaa gatggcgatc tccgcctgga
     7321 tctatcgcag ctgacgttca tggacagcac gggggttcac gtcctggcga ggctggccga
     7381 ccggtgcgcc aacggacacc gggtcgtcgt cgaggggccc acggcccggg ttgcgaagtt
     7441 gttcgagctc gtcggcgctg agagctaccc caacgtggag attcggaacg gccggtccta
     7501 ggtggcctcg tcgaaggttg actagcgatc cagttcgaac cagaccttgt tcccacggtc
     7561 ctcgcggtcg gttccccacc ttgccgcgag ccgatccaca agccggagtc cccaccctcc
     7621 gatcggctcc gagcggatcc gctgatcggg agggtcgaac gccgatccct tgtcgaggac
     7681 ttcgacccga aggcatttcc catcggcgac cttcacgacg atgtcctggg tcgcggccat
     7741 gcccgcatgc accacggcat tggatacgag ctcgctcgtg agcagggcaa gag
//
LOCUS       DATBRK010000222         6609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1044604, whole genome shotgun sequence.
ACCESSION   DATBRK010000222 DATBRK010000000
VERSION     DATBRK010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044604"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VIH70_06770"
     CDS             complement(<1..621)
                     /locus_tag="VIH70_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116099.1"
                     /translation="MRARVMVVLVGLSLVGSACTRGSGSDPPVQDPRARLDALGERWM
                     TSVATITYRTTQRDPGEATSPHQCLRQFVGDTVDRQTGLMICSGTGEMRLAWERSDRW
                     RIDEDAADGSSTSLFGPDEYVRCRFDGAASGCVGVEKPGPFASLVEAPAQTLDELETA
                     AVTAEGPRTIAGMRAECFHATGGSAEAVRRVVWCFSRGGLLLSLFDG"
     gene            687..2018
                     /locus_tag="VIH70_06775"
     CDS             687..2018
                     /locus_tag="VIH70_06775"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018255728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY8116100.1"
                     /translation="MASSGLLTDLYELNMAASYLQRGMGGEATFSLFIRQLPPTRGFL
                     VAAGLEPCLEFLEGFGLSERELDYLGRELGFEDPVLDALGALRFSGEVWAVPEGRVVY
                     ANEPLLEITAPIAVAQLVEPYLLNQITFQTTIASKAARCVLAADGRDVVDFALRRTQG
                     LDAALAVARCSAIVGFAATSNVEAARRYELRPTGTMAHSYIESFASEKEAFRTFARDF
                     PERTTFLVDTYDTMNGVRTAIEVIRELGLAGDLAVRLDSGDLDELSRGARALLDEAGL
                     QGVRIFASGGLDEYQLDRLVRGGAPIDAFGVGTRMGVSADAPSLDSAYKLVEYDGRPM
                     LKLSEHKATEPGRKQVFRGAKGDVIGLRDEPLPDGHEPLLVPVMSGGRRTGPQRTLES
                     ARTLFRADLERVPADAAGIEGPVAPTPRTSGALDRLADDAKADALKRAGLP"
     gene            complement(2019..2801)
                     /locus_tag="VIH70_06780"
     CDS             complement(2019..2801)
                     /locus_tag="VIH70_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8116101.1"
                     /translation="MLHAKKVFDRIWPRVALLATILLVWWAVYETGWIDPSKLPSPAD
                     VWNAFTSNITGSNGLLPAARGSIIRLAFGLSVAVVVGTLIGFAMVASVVVQRSVGTLM
                     TALQALPSIAWLPLAIVWFGFTERAVMFVVFIGGVPAVAIATAISIRQVPPTLVRAAR
                     TMGAKGWTLQRTVVLQACVPGYWTGLQQAWAVAWRALLAAEIIRVGAARGLGHLLEAS
                     AQNTAMLIAVMGVVVVIGLAIDSFFSVMDRRIRSKRGLLVPA"
     gene            complement(2776..3603)
                     /locus_tag="VIH70_06785"
     CDS             complement(2776..3603)
                     /locus_tag="VIH70_06785"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8116102.1"
                     /translation="MTTSRALSIVPEPEPRPVKLSFRGVRKSFTTGTGTEVAVLDDVT
                     FDVRTGEFVCLIGPSGCGKSTILNLLAGLERPDAGEVALDGRPILEPGPDRALLFQDP
                     ALFPWLSVRENVEYALRLKGLGSDEIRERAASWLAKVRLSAFADVQPHELSGGMRHRA
                     ALARALACRPQVLLADEPFGALDAQMREILQKELELVWTELGNTFVFVTHNVREAVFL
                     ADRVIVLSARPGTPIEEYRISTSRPRSFEDVLVAKVVLDIHEHLAKEVRRAAREEGL"
     gene            complement(3600..3818)
                     /locus_tag="VIH70_06790"
     CDS             complement(3600..3818)
                     /locus_tag="VIH70_06790"
                     /inference="COORDINATES: protein motif:HMM:NF021566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HEY8116103.1"
                     /translation="MSTNRPAGATPEPSSSDPAATRPDPEGLTADELDVIRRVRDSLR
                     SIRFGTVLIVVQDGKVVQIETAEKIRLR"
     gene            complement(3815..4933)
                     /locus_tag="VIH70_06795"
     CDS             complement(3815..4933)
                     /locus_tag="VIH70_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEY8116104.1"
                     /translation="MTAAHQMTGTPIRVENLWKRFGGFEAVKDVTFTAEAGSITALLG
                     PSGSGKSTVLRMIAGLEEPTAGRIWMEGEEHTFKTVQERRVGFVFQHFALFRHMTVAQ
                     NVAFGLSVRKQPKTQQRARVQDLLELVQLTGFRDRYPDQLSGGQRQRVALARALAPKP
                     KVLLLDEPFGALDARVRQELRRWLDDLHQELGVTSLLVTHDQDEALELANRIVVMHDG
                     RVEQVGSPGEVYDEPATPFVAGFVGSSSVLHGHVVDGHVQFGDHRVPGAGHLAEGEPA
                     RAFIRPHDVRVTTSPQDGSLPASVERVVNLGWLSRVVLRLPDDQVLTAEIANDELSDI
                     EVGSSVAVDLRRAKAFPSAESGADGGLTSEMTSTAAGS"
     gene            complement(4930..5787)
                     /locus_tag="VIH70_06800"
     CDS             complement(4930..5787)
                     /locus_tag="VIH70_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HEY8116105.1"
                     /translation="MSAPGELRVPVLPSGTRRRLVHGGFVAIALLYVGILVIAPLIGI
                     AWAAFQAGWSTIVSTLKQPDVLHAFYLTGVITVITVTVTSIFGVIVALVIARDRFPGR
                     ALMSAAVDLPLAVSPVIVGLMAVVLFGLGGWFEPWFTSHGIRILFAVPSMVLVTIFIC
                     IPFVIREVVPVLQEVGVTEEEAARTLGASSIQTFFRVTLKNIRWGLLYGIALTTARSI
                     GEIGAVLIVSGQLTGQTETATLYVLRAFDQFQDEQGYIVALTLALVSIVLLVLIEVFK
                     RRQERTQRT"
     gene            complement(5784..>6609)
                     /gene="cysT"
                     /locus_tag="VIH70_06805"
     CDS             complement(5784..>6609)
                     /gene="cysT"
                     /locus_tag="VIH70_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEY8116106.1"
                     /translation="RPSVAIRSALARWGLRGTALLYLGLIVALPTVAVITRGFQDGLQ
                     SLRDALNAPGAREAIVLTLVLAAVTAIINVIFGTLIAYVLVRYEFPGRAWLSTIVDIP
                     FAIPTLVTGVMLRALYGPNSVIGGFLEGQGIHVIFAQLGILLALLFVTLPLVVRTVQP
                     VLLEIDEAEEEAARVLGATRWYTFRRVVLPKLRSGIAAGGLLAFARAIGEFGAVVIVS
                     GNIIGKTLTAPVLIFQLASQFQPSQAAAVATLLFAISFVLVLVTERLVGVRNKDAV"
BASE COUNT         1104 a         2345 c         2254 g          906 t
ORIGIN      
        1 cccgtcgaag agggagagaa ggaggccacc tcgcgagaaa caccacacca ccctgcggac
       61 cgcttcggcc gagccaccag tcgcgtggaa gcattcggct cgcatcccgg cgatcgtgcg
      121 cggcccttcg gccgtcaccg ccgcggtctc gagctcatcg agggtctggg ccggggcctc
      181 gacgagcgac gcgaacggac cgggcttctc gaccccgacg caacccgacg cagctccatc
      241 gaaccggcag cggacgtatt cgtccggccc gaacagcgag gtggagctcc catcggcagc
      301 atcctcgtca atccgccacc gatccgaacg ctcccaggcc agccgcatct cgccggtccc
      361 cgagcagatc atcaatcccg tctgacgatc gacggtgtct ccgacgaact gtcggagaca
      421 ttgatgcggc gaggtcgcct cgccgggatc gcgctgcgtc gtgcgatagg tgatcgtggc
      481 gacggacgtc atccaccgct cccccagggc gtcgagtcgc gcccggggat cctggacggg
      541 cgggtccgat ccgctccccc tcgtgcacgc cgagccgacc agcgacaagc cgacgagaac
      601 gaccatcacc ctggcgcgca tcgagcgcat catgcgcgct cggcctcgtg gattccactt
      661 cgagaaccct cgggtagcct tcggccatgg cgtcgagcgg tctgctgacc gacctgtacg
      721 agctcaacat ggccgcgagc tacctccagc gcgggatggg cggcgaggcg acgttcagcc
      781 tgttcatccg ccagctcccg ccgacgcgcg gcttcctggt cgccgcgggg ctcgagccgt
      841 gcctggagtt cctcgagggc ttcggcctgt ccgagcggga gctggactac ctcggcagag
      901 agctcggctt cgaggacccg gtgctggacg cgctcggcgc gttgcggttc tccggcgagg
      961 tctgggcggt ccccgagggg cgcgtcgtgt acgccaacga gccgctgctc gagatcacgg
     1021 cgccgatcgc cgtcgcccag ctcgtcgagc cctacctgct caaccagatc acgttccaga
     1081 cgacgatcgc gtccaaagcc gcccgctgcg tgctcgcggc ggacggccgc gacgtcgtgg
     1141 acttcgcgct ccgacggacg caggggctcg acgccgcgct cgccgtggcg cgctgctcgg
     1201 cgatcgtcgg attcgcggcg accagcaacg tcgaggcggc gcgtcggtac gagctgcgcc
     1261 ccaccggaac catggcccac tcgtacatcg agtcgttcgc gagcgagaag gaggcgttcc
     1321 gcaccttcgc gcgcgacttc cccgaacgca cgacgttcct cgtcgacacc tacgacacga
     1381 tgaacggcgt ccggacggcg atcgaggtga tccgggagct cgggctcgcg ggcgacctcg
     1441 cggtgcgtct ggacagcggc gatctcgacg agctctcgcg cggggcgcgt gcgctgctcg
     1501 acgaggcggg actgcaaggt gtgcggatct tcgcgagtgg cggtctcgac gagtaccagc
     1561 tcgaccggct tgtccgcggc ggcgcgccga tcgacgcgtt cggcgtcggg acccggatgg
     1621 gggtctcggc cgacgcgccc tcgctcgact cggcctacaa gctcgtcgag tacgacggcc
     1681 gaccgatgct caagctctcg gagcacaagg cgaccgagcc gggccgcaag caggtgttcc
     1741 ggggggcgaa gggcgacgtc atcggcctgc gcgacgagcc gctgcccgac ggccacgagc
     1801 cgctgctggt ccctgtgatg agcggcggcc gccggaccgg cccccagcgc acgctcgaga
     1861 gcgcccgaac gctgttccgg gccgatctcg agcgcgttcc tgccgacgcc gccgggatcg
     1921 agggcccggt ggcaccgacg ccccggacct ccggggcgct cgaccggctc gcggacgacg
     1981 cgaaggccga cgccctcaag cgcgccggac tgccgtagct acgcggggac gagcaacccg
     2041 cgcttgctgc ggatccgtcg gtccatcacc gagaagaagg agtcgatcgc gagaccgatc
     2101 acgaccacca cgcccatcac ggcaatcagc atcgcggtgt tctgggccga ggcctcgagc
     2161 agatggccga gcccacgcgc ggcgcccaca cggatgatct ccgcggccag cagcgcccgc
     2221 cacgcgaccg cccaggcctg ctgcagcccg gtccagtagc ccgggacaca ggcctgcagc
     2281 acgaccgtgc gctgcagcgt ccaccccttg gcccccatcg tccgcgccgc gcggacgagc
     2341 gtgggaggca cctgtcggat cgagatcgcc gtcgcgatcg cgaccgccgg gacgccgccg
     2401 atgaacacga cgaacatcac ggcgcgctcg gtgaacccga accagacgat cgcgagcggc
     2461 agccacgcga tcgacggcaa ggcctgcagg gccgtcatca gcgtgccgac gctgcgctgc
     2521 acgaccaccg atgcgaccat cgcgaacccg atcagggtcc cgacgaccac cgccacgctc
     2581 aggccgaacg ccagccggat gatgctcccc cgcgcggcgg ggagcagccc attcgaaccg
     2641 gtgatgttcg acgtgaacgc gttccagacg tcggcgggcg aggggagctt ggacggatcg
     2701 atccagcccg tctcgtagac ggcccaccac acgagcagga tcgttgccag gagcgccacc
     2761 cggggccaga tccggtcaaa gaccttcttc gcgtgcagca cgccgcacct ccttggcgag
     2821 gtgctcgtgg atgtcgagca ccaccttggc gaccaggacg tcctcgaagc tccgggggcg
     2881 cgacgtcgag atgcgatact cctcgatcgg ggttcccggg cgcgcggaca gtacgatcac
     2941 gcggtcggcg aggaagaccg cctcccgcac gttgtgcgtc acgaacacga acgtgttgcc
     3001 gagctcggtc cacactagct cgagctcctt ctgcaggatc tcgcgcatct gcgcatcgag
     3061 cgcgccgaac ggctcgtccg cgaggaggac ctggggccgg caggcgagcg cccgcgcgag
     3121 cgcggctcga tggcgcatgc cccccgagag ctcatgcggc tggacgtcgg cgaacgccga
     3181 gaggcgcacc ttcgcgagcc agctggcggc cctctcccgg atctcgtcgg atccgagacc
     3241 cttcaaccgg agcgcgtact cgacgttctc tcgcacgctg agccagggga agagcgccgg
     3301 gtcctggaac agcagcgcac gatcgggtcc gggctcgagg atcgggcgac catccagcgc
     3361 aacctcgccg gcatcgggcc gctcgagacc ggcgagcagg ttcaggatcg tcgacttgcc
     3421 gcagccggag ggcccgatca ggcagacgaa ctcgcccgtg cggacgtcga aggtgacgtc
     3481 gtcgagcacg gccacctcgg tgcccgtccc cgtcgtgaac gactttctca ccccgcggaa
     3541 cgagagcttg acggggcgag gctcgggttc gggcacgatc gagagggcgc gggaggtcgt
     3601 catcgcaacc tgatcttctc ggccgtctcg atctggacga ccttcccgtc ctgcaccacg
     3661 atcagcaccg tgccgaaccg gatcgaccga aggctgtcac ggacgcgacg gatcacgtcg
     3721 agctcgtcgg cggtgagccc ttccgggtcc gggcgcgtcg cagccgggtc cgacgaggag
     3781 ggctccgggg tcgcgccggc cgggcggttg gtgctcacga ccccgccgcg gtcgaggtca
     3841 tctccgaagt gaggccaccg tcggccccgg actcggccga ggggaacgcc ttcgcgcgcc
     3901 gcaggtctac ggccacggac gagccgacct ctatgtcgct cagctcgtcg ttcgcgatct
     3961 cggccgtcag cacctgatcg tcggggagcc ggagcacgac cctggagagc cagcccaagt
     4021 tgacgacccg ttcgaccgat gccggcaggg acccgtcctg cggtgacgtc gtcacgcgaa
     4081 cgtcatgcgg acggatgaac gcgcgcgcgg gctcaccctc cgcgagatgg ccggcgccgg
     4141 gaacccggtg gtccccgaac tgcacgtggc cgtcgacgac gtgtccgtgc agcacgctcg
     4201 aggagccgac gaagccggcg acgaacgggg tggccggctc gtcgtagacc tcgccgggcg
     4261 agcccacctg ctccacccgc ccgtcgtgca tgacgacgat ccggttcgcg agctcgagcg
     4321 cctcgtcctg gtcgtgggtc accaacaggc tggtgacacc gagctcctgg tgcaggtcgt
     4381 cgagccatcg ccggagctcc tggcgcaccc gtgcatccag cgcaccgaac ggctcatcga
     4441 gcaggaggac cttgggcttg ggcgcgaggg cgcgcgcgag cgcgacccgt tgccgctgac
     4501 caccggagag ctgatccggg taccggtccc ggaaccccgt gagctgcacg agctcgagga
     4561 gatcctgcac acgagcgcgt tgctgggtct tcggctgctt gcggaccgac aacccgaacg
     4621 cgacgttctg cgccaccgtc atgtggcgga acaacgcgaa atgctggaag acgaagccga
     4681 cccgccgttc ctggacggtc ttgaaggtgt gctcctcccc ctccatccag atccgtcccg
     4741 ccgtgggttc ctccaggccg gcgatcatcc ggagcacggt gctcttgccg gagccgctcg
     4801 gtccgaggag cgcagtgatg ctgcccgcct cggccgtgaa cgttacgtcc ttcacggcct
     4861 cgaacccgcc gaagcgcttc cagaggttct ccacgcggat cggcgtgccg gtcatctggt
     4921 gtgcggccgt catgtccgct gcgtcctctc ctgccggcgt ttgaagacct cgatcaacac
     4981 tagaagaacg atcgagacga gcgccagcgt cagggcgacg atgtacccct gctcgtcctg
     5041 gaactgatcg aacgctcgta ggacgtagag cgtcgccgtc tccgtttggc cggtgagctg
     5101 cccgctcacg atgagcacgg cgccgatctc cccgatcgac cgggcggtgg tgagcgcgat
     5161 cccgtacagg agtccccagc ggatgttctt cagcgtgacg cggaagaacg tctggatcga
     5221 cgaggcgccg agcgtgcgtg ctgcctcctc ctccgtcacc ccgacctcct gcaggaccgg
     5281 gacgacctcg cggatcacga acgggatgca gatgaagatc gtcaccagca ccatcgaggg
     5341 cacggcgaac aggatccgga tgccgtggct cgtgaaccac ggctcgaacc atccgccgag
     5401 tccgaacagc acgaccgcca tcagaccgac gatcaccggt gagacggcga gagggaggtc
     5461 caccgccgcg ctcatcaagg ctcggcccgg gaagcggtcg cgggcgatca cgagcgcgac
     5521 gatcacgccg aagatcgacg tcacggtcac cgtgatgacc gtgatgacgc cggtcaggta
     5581 gaaggcatgg agcacgtcgg gctgcttcag cgtggagacg atcgtcgacc acccggcctg
     5641 gaacgcggcc cacgcgatgc cgatcagcgg ggcgatcacc aggatcccga cgtacaggag
     5701 cgcgatcgcg acgaacccgc cgtggaccag acgtcgacgc gtacccgacg gcaggaccgg
     5761 gacccgaagc tcgccgggtg cgctcatacg gcgtccttgt tgcgaacgcc gaccagccgc
     5821 tcggtcacga gcaccaggac gaacgagatc gcgaacagca gggtggccac ggccgccgcc
     5881 tggctcggct ggaactggct ggcgagctgg aagatcagca ccggggccgt gagcgtcttg
     5941 ccgatgatgt tgcccgagac gatcacgacc gcgccgaact ccccgatcgc gcgggcgaag
     6001 gcgagcagtc ctcccgcggc gatgcccgag cggagtttcg gcaacacgac ccgtcggaac
     6061 gtgtaccagc gggtcgcgcc gaggacccgg gccgcttcct cctccgcctc gtcgatctcg
     6121 agcaggaccg gttgaacggt ccgcaccacc aggggcagcg tcacgaagag cagcgcgagc
     6181 aggatcccca gctgcgcgaa gatcacgtgg atgccctggc cctcgaggaa ccccccgatg
     6241 acggagttcg ggccgtacag cgcgcgaagc atgacgccgg tcacgagcgt ggggatcgcg
     6301 aacgggatgt ccacgatcgt cgagagccac gcgcgacccg ggaactcgta gcgcacgagc
     6361 acgtaggcga tcagggtccc gaagatcacg ttgatgatcg ccgtgaccgc cgccagcacg
     6421 agcgtcagga cgatcgcctc gcgggcgccg ggggcgttca acgcgtcgcg gagggactgg
     6481 aggccgtcct ggaatccgcg ggtgatcacc gccacggtcg gcagggcgac gatcaatccg
     6541 aggtagagca gcgccgtccc ccgcagcccc cagcgcgcga gtgcgctccg gatcgcgacc
     6601 gacggacgc
//
LOCUS       DATBRK010000223         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2199492, whole genome shotgun sequence.
ACCESSION   DATBRK010000223 DATBRK010000000
VERSION     DATBRK010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199492"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VIH70_06810"
     CDS             <1..380
                     /locus_tag="VIH70_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEY8116107.1"
                     /translation="ARPGEEAYGAVSVRVAYGADARVLRRVPADVFWPRPKVDSVLVR
                     LDPRPPAVDVDPAALFAVVDEGFAERRKTMANALRRLGRDAGDAADILRAVDPDPSVR
                     AERLGLDEFASIARAMLERGWTP"
     gene            377..1234
                     /gene="ispE"
                     /locus_tag="VIH70_06815"
     CDS             377..1234
                     /gene="ispE"
                     /locus_tag="VIH70_06815"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY8116108.1"
                     /translation="MTGRTIRRNAHAKINLFLRVLSTREDGYHEIESLVVPISLADRV
                     VVRPAEDLRVEIAGEPAFSPGGLNLALVAALALADACADAAGALVEIDKRIPVAGGLG
                     GGSADAAATLLALNELWGCEVDAATLEEIGERIGSDVPAMLSGGPVLASGRGERLAPA
                     DVPPFHWVLIRFGFGVRSPDAYRWWDEDGAGTGPDPDRLLAVATAGDAEALGPLLFND
                     LERPVAARHPEIEEAARDLIESGALGAVMSGSGSSVIGLARDEAHARELAAGFDGAEP
                     VVGPPLDAA"
     gene            1246..1316
                     /locus_tag="VIH70_06820"
     tRNA            1246..1316
                     /locus_tag="VIH70_06820"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1278..1280,aa:Gln,seq:ttg)
     gene            1395..>1592
                     /locus_tag="VIH70_06825"
     CDS             1395..>1592
                     /locus_tag="VIH70_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY8116109.1"
                     /translation="MPTNSLVAVVLAAGKGKRLKSAVPKVLHPICGRSALWWVLQDVR
                     VARPSKIVIVVHHGAEEVREAV"
BASE COUNT          199 a          552 c          614 g          227 t
ORIGIN      
        1 tcgcgcgccc cggcgaggag gcctacggcg cggtgagcgt ccgggtcgcg tacggggccg
       61 acgcccgcgt gctccgccgg gtgccggcgg acgtgttctg gccgcgtccg aaggtcgact
      121 cggtgctcgt ccggctcgac ccgcggccgc ccgccgtcga cgtggatcca gcggcgctgt
      181 tcgcggtcgt cgacgagggg ttcgccgagc ggcgcaagac gatggcgaat gcgctgcgac
      241 gcctcggccg cgatgccggg gacgccgccg acatcctgcg agcggtcgac ccggacccga
      301 gcgtccgtgc cgagcggctc gggcttgacg agttcgcctc gatcgcgcgc gcgatgctcg
      361 aacgtgggtg gacgccgtga ccgggaggac gatccggcgt aacgcgcacg ccaagatcaa
      421 cctgttcctg cgggtgctct cgacccgcga ggacggatac cacgagatcg agagcctggt
      481 cgtcccgatc tcgctcgccg accgcgtcgt ggtccggccg gccgaggatc ttcgggtcga
      541 gatcgcgggc gagccggcgt tctcaccggg cgggctcaac ctcgcgctgg tcgccgcgct
      601 cgcgctcgcc gacgcatgcg ccgacgccgc cggcgccctg gtggagatcg acaagcggat
      661 cccggtcgcg gggggtctcg gcggcgggag cgccgacgcg gctgccacgt tgctcgcgct
      721 gaacgagctg tgggggtgcg aggtcgacgc cgcgacgctg gaggagatcg gggagcggat
      781 cggttcggac gtccccgcga tgttgtccgg tgggcccgtc ctggcgagtg gacgaggtga
      841 gcgactcgcg ccggccgacg ttccgccgtt ccactgggtg ctgatccggt tcgggttcgg
      901 cgtgaggtcg cccgacgcct atcgctggtg ggacgaggac ggggccggga ccggccccga
      961 ccccgatcgg cttctcgccg tcgcgacggc gggggacgca gaggcgctcg ggcccctcct
     1021 gttcaacgac ctcgagcggc ccgtcgccgc gcggcacccc gagatcgagg aggcggcgcg
     1081 ggacctgatc gagtcgggtg cgctcggcgc ggtgatgagc gggagcggct cctccgtcat
     1141 cggcctggca cgggacgagg cgcacgcccg ggagctggcg gcggggttcg acggcgcgga
     1201 gcccgtcgtg ggtcctccgc tagacgcggc ataatcgaga gccggtccgg ggtcgtctaa
     1261 cggcaagacg cgggcctttg gagccttcga tggaggttcg actcctcccc ccggaactag
     1321 tcctcccgac cggccgtctt gccaggccga ccgcgccgcc ggtacaaagg gagtccgccc
     1381 accgagagga acccgtgccg acgaactcgc tcgttgcggt cgtgctcgcc gcaggcaagg
     1441 gcaagcgcct caagtcggcc gtgcccaagg tcctgcaccc gatctgcggc cggtccgccc
     1501 tgtggtgggt gctgcaagac gtccgcgtcg cgcgcccgtc gaagatcgtg atcgtcgtgc
     1561 atcacggcgc cgaggaggtc cgcgaggccg tc
//
LOCUS       DATBRK010000224         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1480991, whole genome shotgun sequence.
ACCESSION   DATBRK010000224 DATBRK010000000
VERSION     DATBRK010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480991"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VIH70_06830"
     CDS             <1..340
                     /locus_tag="VIH70_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY8116110.1"
                     /translation="ENEVDMARSLIESLTEPWNPEAFTDAYREAMLEIVEKKLAGEPI
                     EAPAEEAPARVVDLMEALKASVAAAKEKTPSAGGARRSGARGQSAKKPAAKRAPAAKK
                     PAARKKASGE"
     gene            422..760
                     /locus_tag="VIH70_06835"
     CDS             422..760
                     /locus_tag="VIH70_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY8116111.1"
                     /translation="MKLVVAIVKPHRLDEVKEALQDLGVHGLTTTDVEGFGRQRGHTE
                     IYRGAEYQVDFVPKIKVEVICEDERVDGVVDAIAKSARTGKIGDGKIWVTPAEQVLRI
                     RTGEMGPDAL"
     gene            762..3227
                     /locus_tag="VIH70_06840"
     CDS             762..3227
                     /locus_tag="VIH70_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY8116112.1"
                     /translation="MSERPAPVGRLLEDLRSLDRAYSTGHHGRWSGRRRSELVDACIR
                     DLAAEARMPEDVALVALGGYGRGALAPGSDLDLLILHDGSSTHDVAALAEHLLYPLWD
                     AGLRVGHAVRTPDACVEIASERLDASTAMLDGRVLEGSEPSWNDVRSRLVKLVRGGPR
                     AFAERLAADREARRDRFGSVSSLLEPELKEGVGGLRDIHALAWLQTAIGAPLEDEGIL
                     RGSERRSVEGAEEFLTRVRCALHLESGRGSDRLLAELQPSIARAMGFADEPGLPAVDG
                     LMRSVFEQARQVEHVSASVFDRFLRGSSLSAPLDPTPGGILRAFAAVARDGDVMPVAS
                     LDALAAVSIDADLPWTEGIRAAFLELLRCGPRAAEGLETLDRVGLLERFVPAWGTVRC
                     RPQRDPYHRSSVDVHLLGSFAGVARLLEDPGDDPSVAPAVQAVPDADGLLLGALLHDI
                     GKTGEGHHVEVGTRVAGETLDHMGVSGPTADLARFLVAEHLLLSDTATRRDLDDERLI
                     VGVADRVGDIGRLAALYLLTMADAEATGPLAWTPWRATLVRELVAKVRHVLQRDDVGR
                     GTAERLTARAEEIRASLAGEDPEAVERFLLRMPRGYVLTVPIDRVPIHHRLLAPAVGA
                     HEVRTHATEGSRAAAYELAVVAADRPGLLSMIAGALSLAGLSILTAQVFTTDDGVAVD
                     LFEIEGVFEPEVGEERWREFRTTLRKAMEGRLSLDHRVREKRRHYPGPPTDVPVRVEV
                     DNDASDFFTVIEVGAPDRLGLLFDITRTLAELQLDVHLAKVATFGGRVVDAFYVRDDL
                     GRRVDDLERVHELEAALRGRLAP"
     gene            complement(3256..>3826)
                     /locus_tag="VIH70_06845"
     CDS             complement(3256..>3826)
                     /locus_tag="VIH70_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY8116113.1"
                     /translation="YTFRRFPTEKSMQTWKAETPETFRFTLKANQRITHWKRLADADQ
                     DVRDFLDLGRLLGERLGTVLYQCPPTLKYDRALIEAFVGYLPPAPRAAMEFRHPSWVE
                     ARDLLLDQGVAWCVAETDENDPGPDDLSWEPFGFLRLRKTEYSDEELRGWVDRIRPSL
                     EAGRDVYCYFKHEDEGHSTQMADRLRAML"
BASE COUNT          519 a         1346 c         1406 g          555 t
ORIGIN      
        1 cgagaacgag gtcgacatgg cccggtcgct gatcgagagc ctgaccgagc cctggaaccc
       61 cgaggccttc acggacgcct accgcgaggc gatgctcgag atcgtcgaga agaagctcgc
      121 cggcgagccg atcgaggcac cggccgagga ggcgcccgcg cgggtcgtgg acctgatgga
      181 ggccctcaag gcctcggtcg cggcggccaa ggagaagacg ccgtctgcgg gaggcgccag
      241 gcggagcggc gcgagggggc agtcggccaa gaagcccgcg gccaagcgcg cgccggcggc
      301 gaagaagccc gcggcgcgca agaaggcgtc cggtgagtga cgaggggcga gtgacgcgga
      361 tcggcacggc cccgaggctc gcgggctagg ctgggcgcgt ccaggccccc gggagcgcca
      421 cgtgaagctc gtcgtcgcga tcgtcaagcc gcatcggctc gacgaggtca aggaggcgct
      481 ccaggacctc ggcgtgcacg ggctcaccac gaccgacgtc gaggggttcg ggcgacagcg
      541 cgggcacacc gagatctacc ggggcgcgga gtaccaggtc gacttcgtcc ccaagatcaa
      601 ggtcgaggta atctgcgagg acgagcgggt ggacggggtc gtcgacgcga tcgcgaagtc
      661 cgcccgtacc ggcaagatcg gcgacggcaa gatctgggtg acgccggccg aacaggtgct
      721 ccggatccgg accggtgaga tgggtcccga cgccctctag cgtgagcgag cggcccgcgc
      781 cggtcggccg gctcctggag gatctccgct cgctcgatcg cgcctactcg accggccatc
      841 acggccggtg gtcgggtcgt cgccgctccg agctcgtcga tgcgtgcatc cgcgacctcg
      901 cggccgaggc ccggatgccg gaggacgtcg cgctcgtcgc gctcggaggg tacggacggg
      961 gggcgctcgc gcccggttcc gacctcgatc tgttgatcct gcacgacggg tcgtcgaccc
     1021 acgacgtcgc ggcgctcgcc gaacacctcc tctaccccct gtgggacgcc ggtctccgcg
     1081 tcggtcacgc ggtccgaacg ccggacgcgt gcgtcgagat cgcgtccgag cggctcgacg
     1141 cgtccaccgc catgctcgac ggccgggtcc tagagggatc cgagccctcg tggaacgacg
     1201 tgcgttcgag gctggtgaag ctcgttcgcg gcggcccccg cgcgttcgcc gaacgcctcg
     1261 cggccgaccg agaggctcgg cgcgatcggt tcgggtccgt gtcgtcgctc ctcgagcccg
     1321 agctcaagga gggcgtcggg ggtctccgcg acatccacgc gctggcctgg ctccagacgg
     1381 cgatcggcgc tccgctcgag gacgagggga tcctgcgcgg gtcggagcgt cgttcggtcg
     1441 aaggagccga ggagttcctc acccgggtcc gctgcgcgtt gcacctggag agcggccgag
     1501 gttcggaccg gctcctcgcg gagctccagc cgtcgatcgc gcgcgccatg ggcttcgccg
     1561 acgagcccgg gctccccgcg gtcgacggac tgatgcgttc ggtgttcgag caggcgcgcc
     1621 aggtcgagca cgtctccgcc tcggtgttcg atcgattcct gcgggggtcc tcgctgtcgg
     1681 ctccgctcga tcccacgccc ggggggatcc tgcgtgcgtt cgccgcggtc gctcgcgacg
     1741 gagacgtgat gccggtcgcc tcgcttgacg cactggcggc ggtgtcgatc gacgccgacc
     1801 tgccctggac cgaaggaatc cgagcggcgt tcctcgagct cctccggtgc ggcccgcggg
     1861 cggccgaggg gctcgagacg ctcgaccgcg tcggcctcct cgaacgcttc gtgccggcgt
     1921 gggggacggt ccggtgccgg ccgcagcgcg acccctacca ccggtcctcg gtcgacgtgc
     1981 acctgctcgg ctcgttcgcc ggcgtcgctc ggctgctgga ggatccgggc gacgatccct
     2041 cggtggcgcc cgcggtacag gcggtccccg acgccgacgg cctgctcctt ggcgcgctcc
     2101 tgcacgacat cggcaagacc ggcgagggcc accacgtcga ggtcgggacg cgcgtggcgg
     2161 gggagacgct cgaccacatg ggcgtgtcgg gtccgacggc cgacctcgcg cgtttcctcg
     2221 tcgcggagca cctcctctta tctgacacgg ccacgcgtcg cgatctcgac gacgagcgcc
     2281 tgatcgtcgg cgtggccgat cgcgtcggtg atatcgggcg cctggccgcg ttgtacctcc
     2341 tcacgatggc cgacgccgag gcgacggggc cgctggcgtg gacgccgtgg cgcgcgaccc
     2401 tggtccgcga gctcgtcgcg aaggtccggc acgtcctcca gcgcgacgac gtgggtcggg
     2461 gcaccgcgga acggctgacc gctcgggcgg aggagatccg tgcgtcgctc gcgggtgagg
     2521 accccgaggc cgtcgagcgc ttcctcctgc ggatgccgcg cggctacgtc ctgacggtcc
     2581 cgatcgaccg cgtgccgatc caccaccgcc tcctcgctcc ggccgtcggc gcgcacgagg
     2641 tgcggaccca cgcgacagag ggatcgcggg cggccgcgta cgagcttgcg gtcgtcgccg
     2701 ccgaccggcc cgggctcctc tcgatgatcg cgggggcgct ctcgctcgcg gggctgtcca
     2761 tcctcaccgc ccaggtgttc acgaccgacg acggcgtcgc ggtcgacctg ttcgagatcg
     2821 agggggtgtt cgagcccgag gtcggggagg aacgctggag ggagttccgg acgacgctgc
     2881 gcaaggcgat ggagggacgg ctctcgctcg atcaccgcgt ccgcgagaag cggaggcact
     2941 acccgggtcc cccgaccgac gtcccggtgc gggtcgaggt cgacaacgac gcgtcggact
     3001 tcttcaccgt catcgaggtc ggcgcgcccg accgcctcgg gctgctgttc gacatcaccc
     3061 gaaccctcgc cgagctgcag ctcgacgtgc atctcgcgaa ggttgcgacg ttcggcgggc
     3121 gtgtggtcga cgccttctac gtccgcgacg acctcgggcg cagggtcgac gatctcgagc
     3181 gggtccacga gttggaggcc gcgctgcgag gccggctcgc accgtgacct cggtccgagc
     3241 cgcgaacgcg ttcggctaga gcatcgcccg gagccgatcg gccatctgcg tgctgtggcc
     3301 ctcgtcctcg tgcttgaagt agcagtagac gtcccggccg gcctccagcg acgggcggat
     3361 ccggtccacc cagccgcgca gctcctcgtc cgagtactcg gtctttcgca gacgcaggaa
     3421 cccgaacggc tcccaggaca ggtcgtcggg tcccgggtcg ttctcgtcgg tctccgcgac
     3481 gcaccaggcg acgccctggt cgagcagcag gtcgcgcgcc tcgacccacg acgggtggcg
     3541 gaactccatc gcggcccggg gcgccggcgg caggtaacca acgaacgcct cgatcagcgc
     3601 gcgatcgtac ttcagcgtgg gcgggcactg gtacagcacg gtcccgagcc gctccccgag
     3661 caaccgcccc aggtccagga agtcgcgcac gtcctggtcg gcgtccgcaa ggcgcttcca
     3721 gtgcgtgatg cgctggttcg ccttcagggt gaaccggaac gtctcgggtg tctcggcctt
     3781 ccacgtctgc atggacttct cggtcgggaa gcgccggaac gtgtag
//
LOCUS       DATBRK010000225         7217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_181303, whole genome shotgun sequence.
ACCESSION   DATBRK010000225 DATBRK010000000
VERSION     DATBRK010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181303"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1748)
                     /locus_tag="VIH70_06850"
     CDS             complement(120..1748)
                     /locus_tag="VIH70_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8116114.1"
                     /translation="MAGPMADPMSGPTARRYDAVVVGGGHNGLTTAAYLARAGRSVLV
                     LERRERVGGILDTIELAPGVRAPGIVHTIGRLRRSVIHDLGLVRHGLVTVEPEARVFA
                     PQPEGPPLTLWSDAARTADELRGRSPADAEAYPGFDRKVRSLASFLSYLHAITPPDVK
                     DPSLADALNGLRLGNAFRRLGKRPGREATRAIPMAIADYVAEAFETDALRGAIASRAV
                     QYTAMGPWSAGTTAVLLADSAGNDGGAPGQSTIARGGPGALAEALANAARGFGAEIGT
                     SSEVVAISVERHRANGVVLADGSEIRARAVITAIDPKRTLTTLLDPVEIGPHLRWRAT
                     NIRTPGSVSKVDLALSRMPSFDGAGPDRLVGRIVIAPSIDHLERAFDASKYGGFGDDP
                     YLEATIPTLSDPTLAPEGTHVMSVVAQWTPFELRETDWAAERDRLGDLVVKALERYAP
                     RLSNLVTARRVTTPADLEREYGLTGGHVYHGEPGLDQFFVWRPLLGHARYRLGLPGLY
                     LCGSGAHPGGGVTGAPGANAAREILGDLKKRSAT"
     gene            complement(1748..3364)
                     /locus_tag="VIH70_06855"
     CDS             complement(1748..3364)
                     /locus_tag="VIH70_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8116115.1"
                     /translation="MALVANRYDAVIIGGGHNGLTAAAYLARAGKTVLVLERRHVLGG
                     AAVTEEVFPGFRFSEFSYVVSLLRPEIIRELELPKHGLEILPLDGTITPLEDDSLWRV
                     NDHGRTIRELRRWSKSDAEAYEEYGLLMADMARFIKPILSVVPPDPGGFNPFDWMPVV
                     GLAKTFRDLPERLQSTFVQLMTMSATEFLRQWFETDPLIATMSASGIIGTFQGVRSPG
                     TAYVLLHHYMGEIDGAFRAWGIPRGGTGGISEAIASAARAAGVEIRTEAPVARIDVRG
                     GRARGVVLESGEEVGAGTVLSSVDSHVTFLRLVEPSNLEPSFLDEVGRYKFRGSSGKV
                     NLALDALPELACRPGRGEWLRSAISFSPGVDYMERAYDDAKYGRFSARPYIDCIIPTL
                     VDPSLAPPGKHVMSCFVQYAPYHLSDGRIWDDAERDAFGETVISTLEERFPRIRDLIL
                     HRQVLTPLDIERITGLTEGNIFQGELSLEQLFFNRPVPGWARYRTPIKDLWMCGSATH
                     PGGGIMGAPGRIAALELLGGRSGGPAGRVA"
     gene            3476..4663
                     /locus_tag="VIH70_06860"
     CDS             3476..4663
                     /locus_tag="VIH70_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY8116116.1"
                     /translation="MSVGTAFHPRTSALNSTMSWGEWSGYFAAAVYADFHDIEYNAIR
                     EQAALIDVSPLYKYELTGPDAARLVDRIVTRDATKLAVGRVMYTPWCNEDGKVVDDGT
                     VARRGEREFFWTAADPCYRWFTMNAAGLDVRIDDVSEDVAAVALQGPRSRDVLEGASR
                     RDWADLRYFGQRRAEIAGVDVHVSRTGYTGDLGYEIWVDAGSATDVWDALWEAGEPHG
                     IRPAGIRALDVARVEAGLILIEAEYTSARHAIGPEQHYSPFEIGLGAFVDLAKPAFVG
                     RHALEREHASGSGRHLVGLDLDWTAIEAMFARHDLPPMVSAQVHRDPVPVYKEGRRVG
                     RATSVTWGPTIKRMVGFGSLLPALADPGTRVSVEWTVEGERGKVGATVVPLPFLDLPR
                     KRA"
     gene            4723..6594
                     /locus_tag="VIH70_06865"
     CDS             4723..6594
                     /locus_tag="VIH70_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8116117.1"
                     /translation="MRSRGVLVLLAVTGGLVALALVATRSLVLGVVAGLLVLLAVVLA
                     VRSRVEEDPSRRGFLLTLGGLGLIAVAAGTAVGAAVRRLTRPDPRPALEAMSTDLGSE
                     YLELVTRSYHAERMGDLQLLVTPFSSSNYPQESRSLAPNDPRSSHAVTWMYGERVPIV
                     VWSPGRIDPQDHTERVTLADLAPTTAALVGFDEFRAEDGRVLPGIPSVTPPAQRRLVV
                     TFVIDGGGWNVLRRWPDAWPNLKRLMREGATYRNAISGSFPAVTAATHATIGTGAFPR
                     THGITGHNVRRGGGPVKAWGEPGHIDPSFLLEPTLADRWTEWTKNEAWVGEIGYQVWH
                     VGMLGRGGRPMGRAPVGVFWDEDGGGGWKSHHPTRYRLPNAAPGLDRLAELTADWEPP
                     PPSPFDPTGKKAMCCFPPVVRYQGELIEATFDSEQVGQDEVADLLYLNFKAPDYAGHV
                     YNMDDERQAEVLGAVDAEVGRVADLLGERFGDGGSVLIVAADHGQCPEIDANGGVRID
                     PIQLEEDINAEFGTSVFGLVQMVAPSEVYLDPKALLDAGLSDQDVAAFLADYRYGENI
                     GPYIRPAAVRRDRLHERSFAAVLPATYIAELARTDLARFGATSYEDADPGGMPAVTW"
     gene            6696..>7217
                     /locus_tag="VIH70_06870"
     CDS             6696..>7217
                     /locus_tag="VIH70_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8116118.1"
                     /translation="MPHRLSQSLQRILDTEEPLRRFFYESRYANRDPADPENCDFVAG
                     NPQEMVLPEYVESLQRWTVPQDKDWYAYKFNEPYAQEAAAAGLRERRGVNFEADDILV
                     TDGAFAALNLCIRTVTDPGDEVIFLTPPWFFYEAIILGGEAHPVRVPVDYETWDLDLD
                     AIEAAITERTTAII"
BASE COUNT         1043 a         2629 c         2533 g         1012 t
ORIGIN      
        1 catcgcgcgt gcttcctcta caccgacctc gcgaacccga cgtccaaccg catctacgtg
       61 gacatcgggt acgagcgcgt ctgcgacgcg atggagttcg tcttccgaac ggcctgaagc
      121 tacgtcgcgg accgcttctt caggtcgccg aggatctcgc gggcggcgtt cgctcccggt
      181 gcgccggtca ccccgccccc gggatgcgcc cccgaaccgc acaggtacag gcccgggagc
      241 ccgagccggt accgggcgtg cccgagcagc ggcctccaga cgaagaactg atccaggccc
      301 ggctccccgt gatagacgtg cccgccggtc aggccgtatt cgcgctccag atcggccggg
      361 gtcgtcacgc gacgcgccgt caccaggttc gagagccgtg gcgcataccg ctcgagcgcc
      421 ttgacgacga ggtcgccgag ccggtcgcgc tcggccgccc agtcggtctc ccgcagctcg
      481 aacggcgtcc actgcgcgac cacgctcatc acgtgcgtcc cctccggagc gagcgtcggg
      541 tccgacagcg tcgggatcgt cgcctccagg taggggtcgt cgccgaagcc gccgtacttc
      601 gaggcgtcga aggctcgctc caggtgatcg atcgagggcg cgatcacgat ccgcccgacc
      661 aggcggtcgg ggccggcgcc gtcgaacgac ggcatgcgcg agagcgcgag gtcgaccttg
      721 gagaccgagc cgggggtccg gatgttcgtc gcccgccagc gcaggtgcgg gccgatctcg
      781 accgggtcca ggagcgtcgt gagcgtccgc ttcgggtcga tcgcggtgat cacggcgcgc
      841 gcccggatct cggaaccgtc cgccaggacc acgccgttcg cgcgatgacg ctcgacggag
      901 atcgcgacga cctcggacga ggtgccgatc tcggcgccga acccgcgggc ggcgttcgcg
      961 agggcctcgg ccagcgcccc tggcccgccg cgggcgatcg tcgactgccc gggagcgccg
     1021 ccgtcgttgc ccgccgagtc ggcgagcagc accgccgtcg tcccggccga ccacgggccc
     1081 atcgcggtgt actgcaccgc acgggacgcg atcgcgccgc ggagcgcatc ggtctcgaac
     1141 gcctcggcga cgtagtcggc gatcgccatc gggatcgcgc gcgtggcctc gcggccgggt
     1201 cgcttgccga gacgccggaa ggcgttcccg agcctcaggc cgttcagcgc gtccgcgagc
     1261 gacgggtcct tcacgtccgg cggggtgatc gcgtgcaggt acgacaggaa cgacgcgagc
     1321 gaccgcacct tcctgtcgaa gcccgggtac gcctcggcgt ccgccggcga acgaccccgc
     1381 agctcgtccg ccgtcctggc ggcgtccgac cagagcgtga gcggcggacc ctcgggttgc
     1441 ggcgcgaaca cgcgcgcctc gggctccacc gtgaccagcc cgtgcctcac gagcccgagg
     1501 tcgtggatca ccgacctgcg aaggcgtccg atcgtgtgga cgatgcccgg cgcccggacc
     1561 ccaggcgcga gctcgatcgt gtcgaggatg ccgccgacac gttcgcgccg ctcgaggacc
     1621 aggaccgacc ggccggccct cgcgaggtag gcggcggtgg tgaggccgtt gtggcccccg
     1681 ccgacgacga ccgcgtcgta ccttcgcgcc gtcgggccac tcatcgggtc cgccatcggg
     1741 cccgccatca ggcgaccctg cccgccggac cccccgagcg tccgcccagg agctcgagcg
     1801 cggcgatccg ccccggcgcg cccatgatcc cgccgccagg atgcgtggcc gagccgcaca
     1861 tccacaggtc cttgatcggc gtccggtagc gggcccaccc ggggaccggc cggttgaaga
     1921 acagctgctc gagcgagagc tctccctgga agatgttgcc ctcggtgagg cccgtgatcc
     1981 gctcgatgtc gagcggcgtc agcacctgtc gatgcaggat caggtcgcgg atcctcggga
     2041 agcgctcctc cagcgtggag atcacggtct caccgaacgc gtcgcgctcg gcgtcgtccc
     2101 atatgcgccc gtccgagagg tggtacggcg cgtattgcac gaagcacgac atcacgtgct
     2161 tgccgggcgg cgccaacgag gggtcgacga gcgtggggat gatgcaatcg atgtaggggc
     2221 gggccgagaa ccttccgtac ttcgcgtcgt cgtacgcgcg ctccatgtag tccacaccgg
     2281 gcgagaagct gatcgcgctg cggagccact cccccctccc cggcctgcac gcgagctcgg
     2341 gcagggcgtc gagcgcgagg ttcaccttcc cgctcgaacc gcggaacttg taccgcccca
     2401 cctcgtccag gaacgacggc tcgaggttcg agggttcgac caggcgcagg aacgtgacgt
     2461 gcgagtccac gctcgacagg acggtgccgg cgccgacctc ctccccggac tccagcacca
     2521 cgcccctcgc ccggccgccc cgaacatcga tccgcgccac cggcgcctcg gtgcggatct
     2581 cgacgcccgc cgctcgtgcc gccgacgcga tcgcctcaga gatcccgccg gtgccgcccc
     2641 gagggatgcc ccacgcgcgg aacgcgccgt cgatctcgcc catgtagtgg tggagcagca
     2701 cgtacgcggt gcccggcgag cgcacgccct ggaacgtccc gatgatcccg gacgccgaca
     2761 tcgtcgcgat cagcgggtcg gtctcgaacc actgccgcag gaactccgtc gcgctcatcg
     2821 tcatcagctg gacgaacgtg gactggaggc gctcggggag gtctcggaac gtcttggcca
     2881 ggcccacgac cggcatccaa tcgaaggggt tgaacccgcc aggatccggc gggacgaccg
     2941 acaggatcgg cttgatgaac cgtgccatgt cggccatgag cagcccgtac tcctcgtagg
     3001 cctcggcgtc gctcttggac caccggcgga gctcgcggat cgtcctgccg tggtcgttga
     3061 cccgccacag ggagtcgtcc tcgagcgggg tgatcgtgcc gtcgagcggc aggatctcga
     3121 gcccgtgctt ggggagctcg agctcgcgga tgatctcggg acgaagcagc gagaccacgt
     3181 aggagaactc gctgaagcgg aagcccggga agacctcctc ggtgaccgcg gcgccgccca
     3241 gcacgtgccg gcgttcgagc acgagcacgg tcttgcccgc gcgggccagg tacgcggccg
     3301 ccgtgagtcc gttgtggcct cccccgatga tgacggcgtc gtaccggttc gccacgagcg
     3361 ccatcgtagg ggcggggtgg ggggcggacc aaccggccct tgactgcagg tcagcccccg
     3421 tccgggcggc ccgctaggct acggccggtc gcgaaaccat cgaggaggat ccccgttgag
     3481 cgtcggcacc gcgttccacc cgcgcacctc ggcgctgaac tccacgatgt cctggggcga
     3541 gtggtccggg tacttcgccg ccgccgtcta cgccgacttc cacgacatcg agtacaacgc
     3601 gatccgcgag caggccgcgc tgatcgacgt ctctccgctg tacaagtacg agctcaccgg
     3661 ccccgatgcc gccaggctgg tcgaccggat cgtgacgcgc gatgccacga agctggccgt
     3721 cgggcgggtg atgtacacgc cgtggtgcaa cgaggacggc aaggtggtcg acgacggaac
     3781 cgtcgcgcgt cggggcgagc gcgagttctt ctggacggcg gccgacccct gctaccggtg
     3841 gttcacgatg aacgcggccg ggctcgacgt gcggatcgac gacgtgtccg aggacgtcgc
     3901 ggccgtcgcc ctgcagggtc cccgctcgcg cgacgtcctc gagggcgcga gccgacggga
     3961 ctgggcggac ctccgctact tcggccagcg ccgcgccgag atcgcgggcg tcgacgtcca
     4021 cgtctcgcga accgggtata ccggagacct cgggtacgag atctgggtcg acgccggttc
     4081 cgcgacggac gtgtgggacg cgctgtggga ggccggcgag ccgcacggca tccgtcccgc
     4141 cggcatccgg gcgctcgatg tcgcccgcgt cgaggccggg ctgatcctca tcgaggccga
     4201 gtacacgagc gcccgtcacg cgatcggccc cgagcagcac tactcgccgt tcgagatcgg
     4261 cctcggcgcg ttcgtcgatc tcgcgaagcc cgcgttcgtc ggccggcacg cgctcgaacg
     4321 cgaacacgcc agcggctcgg gacgtcacct ggtcgggctg gatctggact ggaccgcgat
     4381 cgaggcgatg ttcgcccgcc acgacctgcc cccgatggtc tcggcccagg tgcatcggga
     4441 ccccgtcccc gtctacaagg agggccggcg cgtcgggcgc gcgaccagcg tgacctgggg
     4501 cccgacgatc aagcggatgg tggggttcgg gtcgctgctg ccggcgctgg ccgaccccgg
     4561 cacccgggtc tcggtcgagt ggacagtcga gggcgagcgc ggcaaggtcg gcgccaccgt
     4621 cgtcccgctc cccttcctcg acctgccccg caagcgcgcg taggttcgcg accgaaccct
     4681 cggacggcgc aacaccgaac ctctgagatg atgcgttcgc ccatgcgttc tcgaggcgtc
     4741 ctcgtcttgc tcgccgtcac gggaggcctc gtcgccctgg cgctcgtcgc tacccggagc
     4801 ctcgtgctcg gcgtggtcgc cggactgctc gtcctcctcg ccgtcgtgct ggccgtgcgg
     4861 agccgggtcg aggaggatcc ctcgcggcgc ggcttcctgc tcacgctggg cgggctgggg
     4921 ctgatcgcgg tcgccgcggg gaccgccgtc ggcgcggccg tccgacgcct cacccggccg
     4981 gacccgcggc cggcgctgga ggccatgtcg acggatctcg gttccgagta cctcgagctc
     5041 gtcacccgct cgtaccacgc cgagcggatg ggcgacctgc agctgctggt cacgccgttc
     5101 tcgagctcga actacccgca ggagtcgcgg tcgctcgcgc cgaacgatcc ccggtcgagc
     5161 cacgcggtca cctggatgta cggcgagcgg gtcccgatcg tcgtgtggtc gccgggccgc
     5221 atcgatcccc aggaccacac ggagcgcgtc acgctcgccg acctcgcgcc cacgacggcc
     5281 gcgctggtcg ggttcgacga gttccgcgcc gaggacgggc gggtgctgcc cggcatccca
     5341 tcggtcacgc cgcccgcgca gcggcgcctc gtggtgacgt tcgtgatcga cggtggagga
     5401 tggaacgtac ttcgccgctg gcccgacgcc tggccgaacc tgaagcgtct gatgcgcgag
     5461 ggcgcgacct atcgcaacgc gatctcgggt tcgttcccgg cggtgacggc ggccacccac
     5521 gccacgatcg gcaccggcgc gttcccacgg acccacggca tcaccggaca caacgtccgc
     5581 cggggcggcg gtccggtgaa ggcctggggg gagccgggcc acatcgaccc gtcgttcctg
     5641 ctcgaaccca cgctggccga ccgctggacc gagtggacga agaacgaggc gtgggtcggc
     5701 gagatcggct atcaggtctg gcacgtcggg atgctcggcc gcggcggccg gccgatgggc
     5761 cgggcgccgg tgggcgtgtt ctgggacgag gacggcggcg gcgggtggaa gtcgcaccac
     5821 ccgacgcgct accgcctccc gaacgccgcc cccggcctcg accgcctcgc cgagctcacg
     5881 gccgactggg agccgccgcc cccttccccc ttcgacccga ccggcaagaa ggcgatgtgt
     5941 tgcttcccgc cggtggtgcg ttatcagggc gagctgatcg aggcgacgtt cgactcggag
     6001 caggtcgggc aggacgaggt cgcggacctc ctctacctga acttcaaggc gcccgactac
     6061 gcggggcacg tctacaacat ggacgacgag cggcaggccg aggtcctcgg tgccgtcgac
     6121 gccgaggtcg gccgcgtcgc ggacctgctc ggcgagcggt tcggcgacgg gggcagcgtc
     6181 ctcatcgtcg ccgcggacca tgggcaatgc cccgagatcg acgcgaacgg gggcgtccgg
     6241 atcgacccga tccagctcga ggaggacatc aacgccgagt tcggcacgag cgtgttcggg
     6301 ctggtccaga tggtcgcccc ctccgaggtc tacctggatc ccaaggccct gctcgacgcc
     6361 ggcctgtccg accaggacgt ggcggcgttc ctcgccgact accgctacgg cgagaacatc
     6421 ggcccgtaca tccgcccggc ggcggtccgg cgggaccggc tgcacgaacg ctcgttcgcg
     6481 gcggtgctcc ccgccacgta catcgccgag ctcgcgcgca cggaccttgc gcggttcggc
     6541 gcgacctcgt acgaggacgc ggatcccggg gggatgcccg ccgtcacctg gtagctcggg
     6601 cgaccggtga tagggttcgg cgcacggcca cgcgcggcgt cgcaccccgc gccggccatc
     6661 cacgtgccgc caagaacacg gacggaggac ccgcgatgcc acaccgcctg tcgcagagcc
     6721 tgcagcgcat cctggatacc gaggagccgc tccggcgctt cttctacgag agccgctacg
     6781 cgaaccgcga cccggccgac ccggagaact gcgacttcgt cgccggcaac ccgcaggaga
     6841 tggtgctccc cgagtacgtc gagtcgctgc agcgctggac cgtgccgcag gacaaggact
     6901 ggtacgcgta caagttcaac gaaccgtacg cgcaggaggc cgccgccgcc ggtctgcgcg
     6961 agcgacgcgg cgtgaacttc gaggccgacg acatcctggt gaccgacggc gcgttcgccg
     7021 ccctcaacct gtgcatccga acggtgaccg accccggcga cgaggtgatc ttcctgacgc
     7081 ctccgtggtt cttctacgag gcgatcatcc tcggcggcga ggcgcacccg gtgcgcgtcc
     7141 cggtggacta cgagacgtgg gacctcgacc tggacgcgat cgaggccgcg atcaccgagc
     7201 gcaccacggc gatcatc
//
LOCUS       DATBRK010000226         7302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_181902, whole genome shotgun sequence.
ACCESSION   DATBRK010000226 DATBRK010000000
VERSION     DATBRK010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181902"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VIH70_06875"
     CDS             complement(<1..1089)
                     /locus_tag="VIH70_06875"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8116119.1"
                     /translation="MGSPLVDVAAQLEAKAAAFVKEQRVAGASVGIVHGESLVWSAGI
                     GSADVAANRTPEATTLYRIASITKTFTGTAIMQLRDRGRLDLDDAAVTFIPELRDASS
                     LFGPIETVTIRRMLSHESGLAGDPPGTDWVLPSYEGDVNRNLARVSEMGTRIPPSFQQ
                     KYSNLAYQLLGEIVARVSGVPYVEHVRREIIEPVELHGTAFEPLPEPLLDRRATGYAL
                     ASLTDELAVASVSPAVGAEGGLWSCLEDLGRWISFQMGSTGSHLDTVLDASTLEEMHR
                     PRYLGDEAWTEAWGIAWYARRRDDVIWIQHGGSIHGFKADVCFDPKERIGAIALLNGD
                     ADASRLSMDLATVARAAVLASVPKIEPAT"
     gene            complement(1141..1965)
                     /locus_tag="VIH70_06880"
     CDS             complement(1141..1965)
                     /locus_tag="VIH70_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEY8116120.1"
                     /translation="MDEPGRSSGRVRRMGRLTEPAAEGLLTYGEYLAVPELIGLQRLR
                     SDPPVHDELLFIVVHQAYELWFKQLIFELEAVRDRMFEDDPERARHYLKRVHAIERVL
                     IDHIQVLETMTPQDFLEFRSLLTPASGFQSAQFREVEFLSGLKQPGYLGDLAASPDEL
                     ERLARRLDEPTVWDGFVALLERHGLPMPRDDEEARRESLLRMARDPEHAELFGVAERL
                     LDHDEGFAQWRYHHVLMVEREIGAKAGTGGSSGVEYLRSTLSKRFFPELWGLRSHL"
     gene            complement(1976..3142)
                     /locus_tag="VIH70_06885"
     CDS             complement(1976..3142)
                     /locus_tag="VIH70_06885"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8116121.1"
                     /translation="MVASAPDPGALERLIGFGRDLRRRGLPVGTGRIVTFCRAAGAIG
                     LLERDDLYWAGRSSLISRPEDVEAFDEAFAAWFGTGIRLELPLPGSPPNVDGMGEDER
                     EVEGDGVDDHVIAREWHELEEDALTDGEASIRIVASAAEMLREKSFGDLTEEERLRVT
                     HLIRRLAVQLPMRHTRRYRAAGSGARFDVRRTLRRSLRTQGEPFHRAWRDRGRRARPL
                     VLILDISGSMAPYSRALMQFAFAAMAAGRRVEVFCFGTRLTRVTRTLRTKDPDRALRE
                     IGHLVEDWEGGTRIGASLKELVDGWSQRASIRGSVAVICSDGLERGEPDLLRDQMARF
                     RRLVHKIVWVNPLKGSPHYEPLARGMAAALPSIDEFLPGHNLESLEELSRALAD"
     gene            complement(3123..3998)
                     /locus_tag="VIH70_06890"
     CDS             complement(3123..3998)
                     /locus_tag="VIH70_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8116122.1"
                     /translation="MPFSMVESLQEGLAREHYLADRGLSTAIFLAVSLDKPLLLEGEA
                     GVGKTEVAKVLASLLGRELIRLQCYEGIDASQALYEWNYSKQLIAVRAMQDVTAEANV
                     EDLFGPEYLVERPLLAAIRAGSKAVLLVDELDRADDEFEAFLLEVLSEFAVTIPEIGR
                     VAAEEPPVVIVTSNRTRELHDALKRRCLYHWIDHPALDREIEIIRARAPEVPEALARD
                     VASAVSRLRGFDIAKRPGVAETIDWANAVSFLGAEHLDEEIAAATLGTVLKDHDDQRL
                     AVDRLDEVVHGRERS"
     gene            complement(4051..4908)
                     /locus_tag="VIH70_06895"
     CDS             complement(4051..4908)
                     /locus_tag="VIH70_06895"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY8116123.1"
                     /translation="MLAQTGADQSWFEDWGLPLIVTLATAIVITIAIKIAVARFRRKL
                     EGTPNLTQELNLQRIATLTGALSTTGLTIVWTVAILIILDSLDVPLGPFFASAGIAGV
                     ALGFGAQSIVKDTLTGFFILLENQFGVGNTVELLTTAGPVTGRVEWFTLRATAVRAYD
                     GTLHIVPNGNIQVVSNKSRGWARAIVDTRIAYGQDPEEVRAILDELFDELRDDPQMKD
                     WSMDGAGVLGVETTSDTGVVIRAVADTRPSKKVDVERLMRARITQRLSERGIRAPVLA
                     APPPAAPEA"
     gene            complement(5007..5834)
                     /locus_tag="VIH70_06900"
     CDS             complement(5007..5834)
                     /locus_tag="VIH70_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY8116124.1"
                     /translation="MIPAPFEYLRVDSVEEAIQALSDGTDAKLLAGGHSLLPLMKLRL
                     ARPSLLVDISRLQDLKYVKQDGDRVAIGALTRHHDVANSEVLQELCPIVSYAAGEIGD
                     PQVRHVGTIGGSVAHADPASDMPSVLVALDAEMVVRGPGGVRSVAAGDFFKGLFEPDL
                     ATDEVLTEIRVPPTTGRGWSYLKFHRRAQDWALVGVAALAQNGAGPSVALTNMADRPV
                     RATGVEEELAGGADPAAASQRAAEGTSPPSDAFASAEYRLELVKVLVRRALEEARAR"
     gene            complement(5831..>7302)
                     /locus_tag="VIH70_06905"
     CDS             complement(5831..>7302)
                     /locus_tag="VIH70_06905"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY8116125.1"
                     /translation="EVAATEEGKILGFRVKELCDMGAYFQLLTPGIPELGGWVYMGPY
                     DTQGYWYEFTGVMTNMAPTDAVRGAGRPEATFVVERAVDTVARHVGKDPAEIRRISFA
                     PSHAEAKPSLMGLQIDSADYLPAFERALELADYEQYRKEQAARRESGEVKQIGIGLSS
                     YIEMSGLAPSNILGALRYAAGGWEAAQVRCQPSGKVTLTIGTSPHGQGHDTTWSQIAA
                     DELGVHPDDVEVLHGDTSISPLGMDTYGSRSISIGGAALHVALQKIKDKARTLAAHEL
                     EVAEDDLEFRDGAFRVQGAPDKVRTIPELAVSAWTAHDLPDGYEPDLTAVATWDPKNF
                     TWPFGTHVCVAEVDTETGATQLLRYVAVDDCGVVINPMIVDGQVHGGVAHGIAEALYE
                     EVIYDEAGNLVTGTLTQYLVPSAAEMPAFELDRTETPSPTNPLGVKGIGEAGTIAAPP
                     AVINAIVDAVSHLGVTEIGKPATPERVWKAIRDAKGGAA"
BASE COUNT         1072 a         2644 c         2483 g         1103 t
ORIGIN      
        1 cgtcgccggc tcgatcttcg ggacggacgc cagcacggcg gcccgggcga ccgtcgcgag
       61 gtccatcgac aggcgcgatg cgtcggcatc gccgttgagc agtgcgatgg ctccgatccg
      121 ttccttcggg tcgaaacaga catcggcctt gaatccgtgg atgctgccac cgtgctggat
      181 ccagatcacg tcgtcgcgcc ggcgcgcgta ccatgcgatc ccccaggcct cggtccacgc
      241 ctcgtcgccg aggtatcgcg gacgatgcat ctcctcgagc gtcgaggcat cgaggaccgt
      301 gtcgaggtga gatcccgtcg aacccatctg gaaggagatc caccgtccga gatcctcgag
      361 gcacgaccac aggccgccct cggccccgac cgccggggac accgaggcga cggcgagctc
      421 gtccgtgaga gacgccaacg cgtagccggt cgcgcgccgg tccaggagtg gctcgggcag
      481 cggctcgaac gccgtcccgt gcagctccac cggctcgatg atctcccggc gcacgtgttc
      541 gacgtacggc actccactca cgcgggcgac tatctcgccg agcaactgat aggcgaggtt
      601 cgagtacttc tgctggaagc tcggagggat gcgggtcccc atctcggaaa cgcgcgcgag
      661 gttccggttc acgtcaccct cgtagctggg gagcacccag tcggtgccgg gagggtcccc
      721 cgcgagtccc gactcgtgcg aaagcatccg acggatcgtg acggtctcga tgggaccgaa
      781 gagactcgat gcgtctcgaa gctcggggat gaacgtgacc gcggcgtcgt cgagatcgag
      841 ccgtcctcgg tcccgcagct gcatgatcgc ggtgcccgtg aacgtcttgg tgatcgaggc
      901 gatcctgtac agggtggtcg cttcgggcgt ccggttcgcc gccacatccg cggacccgat
      961 acccgccgac cacacgagcg actccccgtg cacgatcccc accgaggcgc cggccacccg
     1021 ctgctccttg acgaacgcag cggccttcgc ctccagctgt gcggcgacgt caaccagcgg
     1081 gctccccatc cgcgcccttc cgacccgggc ccaaccctac cgtcaccatt ttccggaccg
     1141 ctagaggtgc gagcggaggc cccacagctc cgggaagaac cgcttgctca gcgtcgaacg
     1201 caggtactcc acgcccgagg agccgccggt ccctgccttg gcgccgatct cgcgctcgac
     1261 catgagcacg tggtggtacc gccactgcgc gaacccctcg tcgtggtcga gcagacgctc
     1321 cgccacgccg aacagttcgg cgtgctcggg gtcgcgggcc atccgcagca ggctctcgcg
     1381 ccgtgcctcc tcgtcgtccc tcggcatcgg tagcccgtgg cgttcgagca gcgcgacgaa
     1441 cccgtcccac accgtcggct cgtcgagccg ccgcgcaagc cgctccagct cgtccgggga
     1501 ggcggccagg tcgccgagat acccgggctg cttgagcccg gagaggaact ccacctcgcg
     1561 gaactgggcc gactggaagc cggaggccgg ggtgagcagc gagcggaact cgaggaagtc
     1621 ctgcggggtc atcgtctcga gcacctggat gtggtcgatc agcacgcgtt cgatggcgtg
     1681 gacccgcttg aggtagtggc gggcgcgctc ggggtcgtcc tcgaacatcc ggtcgcgcac
     1741 cgcctccagc tcgaagatca gctgcttgaa ccacagctca tacgcctggt gcacgacgat
     1801 gaacagcagc tcgtcgtgca ccggtggatc cgaacgcagc ctctggagcc cgatgagttc
     1861 ggggacggcc aggtactccc cgtaggtgag gaggccctcc gcggccggct ccgtgagccg
     1921 gcccatccgc cggacccggc cgctggatcg ccccggctcg tccacgagtt ggatgctagt
     1981 cggcgagcgc ccgggagagc tcctcgaggc tctcgaggtt gtggccggga aggaactcgt
     2041 cgatcgaggg cagcgccgcg gccatccctc gtgcgagcgg ctcgtagtgc gggctcccct
     2101 tcaaggggtt cacccagacg atcttgtgga cgagccgacg gaacctggcc atctggtcgc
     2161 ggagcaggtc gggttcgccg cgttcgagcc catcggagca gatcacggcg accgaacccc
     2221 ggatcgacgc ccgctggctc cagccgtcca cgagctcctt gagcgacgcc ccgatccggg
     2281 tgccgccctc ccagtcctcg accaggtggc cgatctcgcg cagcgcccgg tccggatcct
     2341 tcgtgcggag cgtacgcgtg acgcgggtca gccgcgtccc gaagcagaag acctcgaccc
     2401 gccggccggc ggccatcgcg gcgaacgcga actgcatcag cgctcgcgag tacggcgcca
     2461 tcgagcccga gatgtcgagg atcagcacga gcgggcgggc ccggcgtccg cggtcacgcc
     2521 acgcgcggtg gaacggctcg ccctgcgtcc gcagcgaccg gcggagcgtc cggcgcacat
     2581 cgaaccgcgc ccccgatccc gccgcgcggt agcggcgcgt atgccgcatc ggcagctgga
     2641 ccgcgagcct gcggatcagg tgcgtcaccc ggaggcgctc ctcctccgtc aggtcgccga
     2701 acgacttctc gcgaagcatc tcggcggcgc tcgccacgat ccggatcgac gcctcgccgt
     2761 cggtgagcgc gtcctcctcg agctcgtgcc attcccgagc gatcacgtga tcgtcgaccc
     2821 cgtcgccctc gacctcgcgt tcgtcctcgc ccatgccgtc gacgttcggc ggcgagccgg
     2881 ggagcgggag ctcgagacgg atccccgtcc cgaaccacgc ggcgaacgcc tcgtcgaacg
     2941 cctcgacgtc ctccgggcgc gagatcagcg aggatcggcc ggcccaatac aggtcgtcgc
     3001 gttcgagcag gccgatcgcg ccggccgcgc ggcagaacgt gacgatgcgt ccggtcccga
     3061 cgggcagacc ccggcgccga aggtcgcggc cgaacccgat caggcgctcg agcgcgccgg
     3121 gatcaggagc gctcgcgacc atggacgacc tcgtccaggc ggtcgaccgc gagccgctga
     3181 tcgtcgtggt ccttcagcac ggtgccgagc gtggccgccg cgatctcctc gtccaggtgc
     3241 tcggcgccga ggaacgagac cgcgttcgcc cagtcgatcg tctcggcgac gccggggcgc
     3301 ttggcgatgt cgaagccgcg aagccggctc acagctgacg cgacgtcgcg cgcgagggcc
     3361 tccgggacct cgggcgcgcg ggcgcggatg atctcgatct cgcggtcgag cgcggggtga
     3421 tcgatccagt ggtacaggca ccggcgcttg agcgcgtcgt ggagctcgcg cgtccggttc
     3481 gaggtcacga tgaccacggg cggctcctcc gcggccacgc ggccgatctc ggggatcgtg
     3541 acggcgaact cggacaggac ctcgagcagg aacgcctcga actcgtcgtc cgcgcgatcg
     3601 agctcgtcga cgagcaggac ggccttcgag ccggcgcgga tcgccgcgag cagcgggcgc
     3661 tcgacgaggt actcgggccc gaacaggtcc tcgacgttgg cctcggcggt cacgtcctgc
     3721 atcgcgcgga cggcgatcag ctgcttggag tagttccatt catagagcgc ctggctcgcg
     3781 tcgatgccct cgtagcactg gagccggatc agctcgcgac ccaggagcga cgcgagcacc
     3841 ttggcgacct cggtcttgcc gacgccggcc tcaccctcca gcagcaacgg cttgtcgagc
     3901 gagacggcga ggaagatcgc cgtcgacagc ccccggtcgg ccaggtagtg ctcgcgagcg
     3961 agcccctcct ggagcgactc gaccatgctg aacggcatcc gacccatcgt gccgaaagcc
     4021 taccggccgc ccgggcttga gagcggagcc ttacgcctcg ggagcggccg gcggcggggc
     4081 cgcgaggacc ggtgcgcgga tccctcgctc ggagagccgc tgggtgatgc gggcgcgcat
     4141 gagtcgctcg acgtcgacct tcttcgacgg acgggtgtcg gcgacggctc ggatcacgac
     4201 gccggtatcg ctcgtggtct cgacgccgag cacgccggca ccgtccatcg accagtcctt
     4261 catctgggga tcgtcgcgaa gctcatcgaa gagctcgtcg aggatcgccc ggacctcctc
     4321 ggggtcctgg ccgtacgcga tgcgcgtgtc gacgatcgca cgtgcccagc cccgcgactt
     4381 gttgctgacg acctggatgt tgccgttcgg gacgatgtgc aacgtcccgt cgtacgcgcg
     4441 gacggcggtg gcgcgcagcg tgaaccactc gacccgaccg gtcaccggac cggccgtggt
     4501 caggagctcc acggtgttcc cgacgccgaa ctggttctcc aacaggatga agaaccccgt
     4561 gagcgtgtcc ttcacgatgc tctgtgcacc gaaacccaac gcgacgccgg cgatccccgc
     4621 gctggcgaag aacggcccga gcggcacgtc gaggctgtcg aggatgatga ggatcgcgac
     4681 cgtccagacg atcgtcaggc ccgtggtcga gagcgcgccg gtgagcgtgg cgatccgctg
     4741 caggttgagc tcctgcgtca ggttcggggt cccctcgagc ttgcgacgga accgtgcgac
     4801 ggcgatcttg atcgcgatcg tgatcacgat cgcggtcgcc agcgtcacga tgagggggag
     4861 cccccagtcc tcgaaccacg actggtccgc accggtctgc gcgagcatgc cctccatcta
     4921 ggcagacggg cccgcgagcg tcaaagaccg aggccgggcg cagtgcgccc ggcctcgatc
     4981 tgccgtgcgg ggcgtttccc cgacgtctat ctcgcccgcg cctcctcgag ggctcggcgc
     5041 accaacacct tcaccagctc caggcggtac tcggcgctcg cgaacgcgtc gctcggcggg
     5101 ctcgtcccct cggcggcgcg ctgcgacgcg gcggccggat cggcgccacc ggccagctcc
     5161 tcctccacgc ccgtcgcccg cacggggcgg tccgccatgt tcgtgagcgc gaccgagggt
     5221 ccggcgccgt tctgcgcgag cgcggcgaca cccacgagcg cccagtcctg cgcgcggcgg
     5281 tggaacttca ggtagctcca gcctcgaccc gtcgtcgggg ggacgcggat ctcggtcagg
     5341 acctcgtcgg tcgccaggtc gggttcgaac aggcccttga agaagtcgcc cgccgcgacg
     5401 ctccgaacgc ccccgggccc ccggacgacc atctcggcgt cgagggccac gagcacgctc
     5461 ggcatgtccg acgccgggtc ggcgtgcgcc accgaaccac cgatcgtccc gacgtgccgc
     5521 acctgcgggt cgccgatctc gcccgccgcg tacgacacga tgggacacag ctcctgcagg
     5581 acctcgctgt tcgccacgtc gtggtgccgc gtgagcgccc cgatcgcgac ccggtctccg
     5641 tcctgcttca cgtacttcag gtcctgcagc cgtgagatgt ccaccagcag cgacggcctc
     5701 gccaggcgga gcttcatcag cggcaggagc gagtgaccgc cggcgaggag cttcgcgtcc
     5761 gttccgtcgg acagcgcctg gatcgcctct tcgaccgagt ccacgcgcag gtactcgaag
     5821 ggcgccggga tcatgccgca ccccccttcg cgtcgcggat cgctttccac acgcgctcgg
     5881 gcgtggccgg cttgccgatc tcggtcacgc cgaggtgcga caccgcgtcc acgatcgcgt
     5941 tgatcactgc gggtggggcc gcgatcgtgc cggcctcgcc gatccccttg acgccgagcg
     6001 ggttcgtcgg cgagggcgtc tcggtccggt cgagctcgaa cgccggcatc tcggcggcgg
     6061 agggaacgag gtactgcgtc agcgtccccg tgaccaggtt gccggcctcg tcgtagataa
     6121 cctcctcgta cagggcctcc gcgatcccgt gagccacccc gccgtgcacc tgcccgtcga
     6181 cgatcatcgg gttgatcacg acgccgcagt cgtcgaccgc cacgtagcgg agcagctggg
     6241 tcgctcccgt ctcggtgtcg acctcggcga cgcagacgtg ggtcccgaac ggccaggtga
     6301 agttcttcgg gtcccaggtc gcgaccgcgg tgagatcggg ctcgtagccg tcggggaggt
     6361 cgtgcgccgt ccacgccgag acggccagct cggggatcgt tcggaccttg tcgggcgcgc
     6421 cctgtacacg gaacgccccg tcccggaact cgaggtcgtc ctccgccacc tcgagctcgt
     6481 gcgcggccag cgtccgcgcc ttgtccttga tcttctgcaa cgcgacgtgg agcgccgcgc
     6541 cgccgatcga gatcgagcgc gagccgtacg tgtccatccc gagcggggag atcgacgtgt
     6601 cgccgtgcag gacctccacg tcgtcggggt ggacacccag ctcgtcggca gcgatctgtg
     6661 accaggtggt gtcgtggccc tggccgtggg gcgaggtgcc gatggtgagc gtgaccttgc
     6721 cgctcggttg gcaccggacc tgcgccgcct cccagccgcc ggccgcgtac cggagcgcac
     6781 ccaggatgtt cgacggcgcc agcccggaca tctcgatgta gctggagagc ccgatcccga
     6841 tctgcttcac ctccccgctc tcgcggcgag ccgcctgctc cttgcggtac tgctcgtagt
     6901 ccgcgagctc gagcgcgcgt tcgaacgccg ggaggtagtc cgccgaatcg atctgcaacc
     6961 ccatcagcga cggcttcgcc tccgcgtggc tcggcgcgaa gctgatccgc cggatctcgg
     7021 ccgggtcctt gccgacgtgc cgcgccaccg tatccaccgc gcgttcgacg acgaaggtcg
     7081 cctcgggccg tccggctccc cggacggcgt ccgtgggcgc catgttcgtc atgacgcccg
     7141 tgaactcgta ccagtagccc tgggtgtcgt aggggcccat gtacacccag cccccgagct
     7201 cggggatccc cggcgtgagg agctggaagt acgcgcccat gtcgcacagc tccttcaccc
     7261 ggaacccgag gatcttcccc tcctcggtcg cggcgacctc ga
//
LOCUS       DATBRK010000227         6863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_182012, whole genome shotgun sequence.
ACCESSION   DATBRK010000227 DATBRK010000000
VERSION     DATBRK010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182012"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..560
                     /locus_tag="VIH70_06910"
     CDS             132..560
                     /locus_tag="VIH70_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8116126.1"
                     /translation="MRRGLPGGYELDDDPGRLDLDAIWDFLSNEAYWGTDRSRDVVER
                     QVREAARTVGLYHEGRQVGFARVVSDGVIAYLADVYVVGEHRGHGLGVELVRASVDEG
                     PQRDLRWLLHTRDAHGLYERFGFGPPSERLLERPPAGERR"
     gene            complement(568..915)
                     /locus_tag="VIH70_06915"
     CDS             complement(568..915)
                     /locus_tag="VIH70_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8116127.1"
                     /translation="MDDRGATETEGHICEHFQHAAEILGRRWSPQVIRVLLYGPARFG
                     ELRERVPGISDTLLSERLKQLEAEGIVLRTVHAGRPVLIEYSLTESGAGLCTVFEALG
                     EWAERFAGADAPA"
     gene            1057..1632
                     /locus_tag="VIH70_06920"
     CDS             1057..1632
                     /locus_tag="VIH70_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8116128.1"
                     /translation="MATETPEVIDRSALPPAGTWTFDKNHTRLEFVARHMLSKVRGRF
                     TEFDGTVTIGERPEESRVEVEMQTASITTDTGMRDDHLRSDDFLEVEEHPVMTFRSTE
                     LRPTGENTFQLVGDLTIKGITRQVVLDAEFNGWGPGARPDAPPLAAFSAKTEINREDW
                     DMTWNVAVETGGFLVGKKVQIEIETELNPQQ"
     gene            1988..2800
                     /locus_tag="VIH70_06925"
     CDS             1988..2800
                     /locus_tag="VIH70_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018252029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY8116129.1"
                     /translation="MRLALCQTNCGQDVAVNEEQVFGLLERAAADGADLAALPEVWPR
                     QGSAKQIRETAEPVPGRRTDLLAEVAARHRIWVHGGSVLERDGQRIFNTSVLFDRSGE
                     LVATYRKIHLFDADPPGAVPSRESSVFSAGDQIVTAETEFGRVGLSICYDLRFPELYR
                     ALAVRGATILFVPAAFRFETGVDHWEPLLRARAIEDQAFVVAAAQWGTWGPPGRERRN
                     FGHSLVADPWGNLVAQATDGVGVTFAELDLARVEQVRQILPSLRHRRLQPSC"
     regulatory      2993..3081
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3138..3599
                     /locus_tag="VIH70_06930"
     CDS             3138..3599
                     /locus_tag="VIH70_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY8116130.1"
                     /translation="MTKSGNRSRRRMALVFLLAAVLVSSGIAITPALAGSGLRGRMAP
                     LINADRADRDRTALKLDLELSRYAKRHSQDMADAGELFHTDDLAAKLKGKHWSIGGEN
                     VGMGPSLPDLEDMFMASTPHRRNILNREFDHFAVGVVESNGNFWVTVIFYG"
     gene            complement(3674..4243)
                     /locus_tag="VIH70_06935"
     CDS             complement(3674..4243)
                     /locus_tag="VIH70_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY8116131.1"
                     /translation="MISALVLAAGEGTRFGGTKQLEILRGKPLVQYAVDAAAAADVGE
                     IVVVLGHDAIRVRGALDLPEAARAVVNERYADGQSTSLAVGLRALHPASDAAIVLLAD
                     QPGIAARHVRALETAFEADGPEILRIRFRDGPGPAILARSVWDEAMRLTGDVGARALF
                     ESEPARARWLDVDEDAPPDVDRRADLERA"
     gene            complement(4240..4851)
                     /locus_tag="VIH70_06940"
     CDS             complement(4240..4851)
                     /locus_tag="VIH70_06940"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8116132.1"
                     /translation="MEIENEFDVPAPVDHVWAYLLDVERVAPCMPGAELTEVVDDHTW
                     KGKVNMKLGPVSLAFAGTVTMQERDDQSKRIVLAAKGMEQRGKGAANASVTSWLERGD
                     GVTNVKMRADIHLTGTVAQLSRGLLPEVSRKLTQQFADCLLQSMGAAEVRATESADVA
                     ASVAAPVEASTRTKPIGGIRLGLAAIWASIKGFFRRLFGKREA"
     gene            5021..5464
                     /locus_tag="VIH70_06945"
     CDS             5021..5464
                     /locus_tag="VIH70_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8116133.1"
                     /translation="MAVTATTDRIVDRAEQLRDDGIDAAGFTADLTDEQQARGLVRAV
                     ETRFGRLDVLVNNAGMVQSGLEGVWASFVDLSAPAFGHHLGLNLWTAFFVTRAALPGM
                     IERGYGRIVMVSSVTGPVVTNPERRLRRGLGSDGRSHAVGGDRDG"
     gene            5445..5705
                     /locus_tag="VIH70_06950"
     CDS             5445..5705
                     /locus_tag="VIH70_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8116134.1"
                     /translation="MAIETARSGVTCNSVQPGWIQTGSQEAVEAVAGRNTPVGRSGRP
                     EEVAEAMAFLASAGASYVTGQALVVDGGNTIQEYKGPPADWY"
     gene            complement(5702..6844)
                     /locus_tag="VIH70_06955"
     CDS             complement(5702..6844)
                     /locus_tag="VIH70_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY8116135.1"
                     /translation="MSELTDVLSAIESLSARGSKLALATIVAVSGSTYRRPGARLLVP
                     EEGELVGNISGGCLENDVADVAKVVMTEGIARIVSFDLTADDDAVWGWGLGCNGAFEL
                     FVEPADKAAEVAGALRMALDEERPISMVTVLDADVPGVEQGNRMLVKPDRVAERSLGD
                     AVLDRAARAAALELLGAERSEVRTLSGSSGRARAFVEVLEPPLRLLICGAGHDAAPLV
                     EAAADLGWNPIVVDDRPEFLSRERFPKAADLVLVERPDKVAEVAPVDARTYVVVMTHN
                     FLRDKDYIRSLLASPARFLAMLGPAVRTERLLIELRDEGVDISDDDRARIRSPAGLDL
                     GAEGAEEIAAAVVAEIVAVKRGRGAGFLRDRRGPIHERPARLQPSS"
BASE COUNT         1042 a         2380 c         2418 g         1023 t
ORIGIN      
        1 ccgatcccgg ggttccgcgc ggtcgccgaa cgactcgagg cgatgctccc cgccggcagg
       61 ggcgacctcg actacgccgc gctcgtcacg ctcctcgagg acgaggcggg cgtcgacctt
      121 cgagccgagt cgtgaggcgc gggctccccg gcgggtacga gctcgacgac gaccccggcc
      181 gccttgatct ggacgcgatc tgggacttcc tgtcgaacga ggcgtactgg ggcaccgacc
      241 gttcgcgcga cgtggtcgag cgacaggtgc gcgaggccgc gcggacggtc ggcctgtacc
      301 acgagggccg gcaggtgggg ttcgcgcgcg tggtgagcga cggggtgatc gcgtacctcg
      361 ccgacgtcta cgtcgtgggg gagcaccggg gtcacgggct gggcgtggag ctcgtccgcg
      421 ccagcgtcga cgaggggccc cagcgcgacc tgcggtggct gctccacact agggacgcgc
      481 acggcctgta cgagcgattc gggttcggcc ccccgagcga acggctcctc gagcgcccac
      541 cggccggcga gcgccggtga ccgggcctca ggcgggcgcg tcggcgccgg cgaaccgctc
      601 cgcccactcg cccagcgcct cgaagaccgt gcagagcccc gcgccggact cggtcagcga
      661 gtactcgatc agcacggggc ggccggcgtg cacggtccga agcacgatcc cctcggcctc
      721 gagctgcttc aggcgttcgg agagcagggt gtccgagatc cccggcacgc gctcgcgaag
      781 ctccccgaac cgcgcgggcc cgtacaggag gacccggatg acctgtgggc tccatcgccg
      841 gccgaggatc tcggccgcgt gctggaagtg ctcgcagatg tgcccctcgg tctcggtcgc
      901 gccacgatcg tccacgagag gcatcgtagc cggtggcgct tcggaaacct cgttacctac
      961 gggaatagca ggaactctca tctcgttagg gggaacgata tgcggaggcg gtctcttccc
     1021 ggaaccggga agagccccga ggaggaggag gtatcgatgg caacggagac gcccgaggtg
     1081 atcgaccgga gcgcgctgcc ccccgccggg acgtggacgt tcgacaagaa ccacacccgg
     1141 ctggagttcg tggcccggca catgctctcg aaggtgcggg gccggttcac ggagttcgac
     1201 ggcacggtga cgatcggcga gcgacccgag gagtcccgcg tcgaggtcga gatgcagacc
     1261 gcaagcatca ccaccgacac gggcatgcgg gacgaccacc tgcggagtga cgatttcctg
     1321 gaggtcgagg agcacccggt catgacgttc cggagcacgg agctgcgccc gaccggtgag
     1381 aacacgttcc agctggtggg cgacctgacg atcaagggca tcacgcgcca ggtggtcctc
     1441 gacgccgagt tcaacggctg gggcccgggc gcgaggcccg acgctccgcc gctggcggcc
     1501 ttctcggcga agaccgagat caaccgcgag gactgggaca tgacctggaa cgtcgcggtc
     1561 gagaccgggg ggttcctggt cggcaagaag gtccagatcg agatcgagac cgagctcaac
     1621 ccgcaacagt agaggtggac cccctcgcct tgcgtcggcc tgcgccccaa gccaagcggg
     1681 ggcgcaggtc gttcacggtt gcgtgtcagg acatgccggc gacgagactt catcccacct
     1741 cggcggagtg ttgggggaat ctcacgcagt tccgttcgcc tgggatccat cgctgttctg
     1801 ctctcgcttc atcgaccgga ctgggacttc cgcttcgatc catgtcgccg cactcgaggg
     1861 gttttccaca gtgaaggcac actctcagtc ctgacatcga cctacgacag acgccgcacc
     1921 cccattgcag tgaagtgaaa cgttggcagt ggcgcggggc ggatccatcc ctcgtacgat
     1981 tcgtccgatg cggttagccc tctgtcagac caactgcggc caggacgtcg ccgtcaacga
     2041 ggagcaggtg ttcgggctgc tcgagcgcgc cgccgctgac ggggccgacc tcgcggcgct
     2101 gccggaggtc tggcctcggc agggctcggc gaagcagatc cgcgagacgg ccgagcccgt
     2161 tcccggtcgc cggaccgacc tgctcgccga ggtcgccgcc cggcatcgga tctgggtgca
     2221 cggggggagc gtcctcgaac gcgacggcca gcggatcttc aacacgagcg tgctgttcga
     2281 ccgctcgggc gagctcgtcg cgacctaccg caagatccac ctcttcgacg ccgatccccc
     2341 cggcgcggtc ccgagccgcg agtcgtccgt cttctccgcc ggtgaccaga tcgtgacggc
     2401 cgagaccgag ttcggccggg tcggcctgtc gatctgctac gaccttcggt tccccgagct
     2461 gtaccgggcg ctcgccgtcc ggggcgcgac gatcctgttc gtgccggccg cgttccgctt
     2521 cgagaccggc gtggatcact gggagccact gctccgggca cgagccatcg aggatcaggc
     2581 gttcgtggtg gccgccgcgc agtgggggac ctgggggcca cccggccggg agcggcgaaa
     2641 cttcggccac agcctggtcg ccgacccgtg ggggaacctg gtggcccagg ccaccgacgg
     2701 ggtcggggtc accttcgcgg agctggatct cgcccgggtc gagcaggtcc gtcagatcct
     2761 gccgtccctg cgccaccgga ggctgcagcc ctcctgttga gcccctccca cccgttaagc
     2821 ccttgagacc cattacgccc gcccggtatc taggcctttc actcggtaag cctttcggcc
     2881 gatggtccga tgcccggtcc gtcgctcatc cgggctagcg cttccggctc gaaccactcg
     2941 acgacactca gttgccctac cgctcgcacc gataaccccc ccgaagggtt ggtcccgata
     3001 acggcaaagc accgggaacg gtgcgacgca aagctccggg ccctaccgct cacaaggagc
     3061 gggggcggcc gagctgccga aggaccgggt ctctatccgg agcccgagcc tgcagggacc
     3121 ctgacgaggg aggcggcatg acgaagtcgg gcaaccggag ccggcgccgg atggcgcttg
     3181 tgttcctgct cgcggccgtc ctcgtatcgt cggggatcgc gatcaccccc gctctcgctg
     3241 gcagcgggct gcgcggccgg atggccccgc tcatcaacgc cgaccgggcg gacagggacc
     3301 gcacggcgct gaagttggat ctcgagctgt cccgttacgc gaagaggcac tcgcaggaca
     3361 tggcggacgc gggagagctc ttccacaccg acgatctcgc agccaagctg aaaggcaagc
     3421 actggtccat cggaggcgag aacgtcggca tgggtcccag cctcccggac ctcgaagaca
     3481 tgttcatggc ttcgacgccg caccgccgga acatcctgaa cagggagttc gaccacttcg
     3541 cggtcggcgt cgtcgagtca aacgggaact tctgggtgac cgtcatcttc tacggctagc
     3601 ccctcccgac ctagctgtag agacgaggcg ccccggtcgc aaaggtgacc ggggcgcctc
     3661 acttcgttcg ccttcaggcg cgttcgaggt cggcgcggcg gtcgacgtcg ggtggggcgt
     3721 cctcatcgac gtcgagccag cgggcgcgcg cgggctccga ctcgaagagg gcgcgggcgc
     3781 cgacgtcgcc ggtcagcctc atcgcctcgt cccagaccga gcgcgcgagg atcgccgggc
     3841 ccggcccgtc ccggaaccgg atccgcagga tctcgggccc gtccgcctcg aacgcggtct
     3901 ccagcgcccg gacgtggcgg gccgcgatac cgggctgatc cgcgagcagg acgatcgcgg
     3961 cgtcgctcgc ggggtggagc gcccggagtc cgacggcgag cgaggtcgac tgaccgtccg
     4021 cgtaacgttc gttcacgacg gcccgggctg cctcgggcag gtcaagcgct ccgcgcacgc
     4081 ggatcgcatc gtggccgaga accacgacga tctcgccgac gtccgccgcc gccgccgcgt
     4141 cgaccgcgta ctgcacgagt ggcttgccgc ggaggatctc gagctgcttg gttccgccga
     4201 accgcgtgcc ctcgcccgcc gccaggacga gggcggagat cacgcttcgc gtttgccgaa
     4261 cagacgccgg aagaagccct tgatcgacgc ccagatcgcg gcgagcccga gacggatccc
     4321 tccgatcggc ttggtccgcg tggacgcttc gacgggggcg gccaccgagg cggcgacatc
     4381 ggcggactcg gtggcccgca cctcggcggc gcccatgctc tgcaggagac agtcggcgaa
     4441 ctgctgcgtg agcttgcggc tgacctcggg cagcaggccc cggctcagct gcgccacggt
     4501 acccgtcaga tggatgtcgg cgcgcatctt cacgttcgtc acgccgtcgc cccgctcgag
     4561 ccagctcgtc accgaggcgt tggcggcccc cttcccccgc tgctccattc ccttggcggc
     4621 cagcacgatg cgcttggact gatcgtctcg ctcctgcatc gtgaccgtcc cggcgaacgc
     4681 gagcgagacc ggcccaagct tcatgttgac cttgcccttc caggtgtggt cgtcgacgac
     4741 ctcggtgagc tcggcgccgg gcatgcacgg cgccacgcgc tccacgtcca gcaggtacgc
     4801 ccagacgtga tcgacgggcg cggggacgtc gaactcgttc tcgatctcca tcgatgctcc
     4861 ttgccgtccc cgcgagccta tcgcgagcaa gacgtcgctc gctggtaacg tcggcgcgca
     4921 tgggtcgttt cgacgaacgt gtggccctgg tcaccggcgc cgggagtccc gatgggatcg
     4981 ggttcgcgac ggctcggttg ttgggctcgg ctgggcccgg gtggccgtca cggcgacgac
     5041 ggaccgcatc gtcgatcgtg ccgaacaact gcgggacgac gggatcgatg cggccgggtt
     5101 caccgccgac ctgaccgacg agcagcaggc ccgcgggctg gtccgggccg tcgaaactcg
     5161 cttcgggcgc ctcgatgtct tggtcaacaa cgcggggatg gttcagagcg gtctggaggg
     5221 tgtgtgggcc tcgttcgtgg acctctccgc accggcgttc gggcaccacc tcgggttgaa
     5281 cctctggacg gccttcttcg tcacgcgagc ggccctccct ggaatgatcg aacgtggcta
     5341 cgggaggatc gtgatggtga gttcggttac cggaccggtc gtcacgaacc cggagcgcag
     5401 gctacggcgc gggctaggca gcgatggacg gtctcatgcg gtcggtggcg atcgagacgg
     5461 ctaggagcgg cgtcacgtgc aattcggtcc agcccggatg gatccagacg ggctcgcagg
     5521 aggcggtaga ggcggtcgcg ggccgcaaca ctccggtagg ccgaagcggc cgaccggagg
     5581 aggtggcgga ggcgatggcg ttccttgcct cggcgggcgc gagttacgtc accgggcagg
     5641 cgctggtcgt cgacggtggc aacaccatcc aggaatacaa gggaccgccc gcggactggt
     5701 attaagagga cggttgtagg cgcgcggggc gttcgtggat cggcccccgg cgatccctga
     5761 ggaacccggc gcctcgtccc cgcttcacgg ccacgatctc ggcgacgacc gcggcggcga
     5821 tctcctccgc gccctcggcg ccgaggtcga gcccggcggg actccggatc cgcgcacgat
     5881 cgtcatccga gatgtcgacg ccctcgtcgc ggagctcgat cagcagtcgc tcggtccgga
     5941 ccgcggggcc cagcatcgcg aggaagcgcg ccggcgaggc gagcagcgag cggatgtagt
     6001 ccttgtcgcg caagaagttg tgggtcatca caacgacgta cgtgcgcgcg tcgaccgggg
     6061 cgacctcggc gaccttgtcc ggacgctcga cgagcacgag gtccgcggcc ttggggaagc
     6121 gctcgcggct gaggaactcc ggccggtcgt cgacgacgat cgggttccat cccaggtccg
     6181 ccgccgcctc gaccagcggc gcggcatcgt ggccggcgcc gcagatcagc agccgcagcg
     6241 gcggctcgag cacctcgacg aaggcgcgcg cgcggccgct cgagccggag agggtgcgca
     6301 cttcgctgcg ttcggccccc aggagctcga gtgcggcggc gcgagcggca cggtcgagca
     6361 cggcgtcgcc gagcgatcgt tcggcgacgc ggtccggctt cacgagcatc cggttgccct
     6421 gctccacccc ggggacgtcg gcgtcgagca ccgtcaccat cgagatcggt cgttcctcat
     6481 ccagggccat ccgcagcgcg cccgcgacct cggcggcctt gtcggcgggc tcgacgaaca
     6541 gctcgaacgc cccgttgcac ccgagccccc aaccccacac ggcgtcgtcg tccgcggtga
     6601 ggtcgaacga cacgattcgc gcgatcccct cggtcatcac gaccttcgcg acgtcggcga
     6661 cgtcgttctc caggcagccg cccgagatgt ttccgacgag ctcgccctcc tccgggacga
     6721 gaagccgggc gcccgggcga cggtaggtcg agccgctcac ggccacgatc gtcgcgagcg
     6781 cgagcttgct cccgcgcgcc gagagcgact cgatcgccga caggacgtcg gtgagctcgc
     6841 tcatgggtcc gatggtacgc gcg
//
LOCUS       DATBRK010000228         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2058823, whole genome shotgun sequence.
ACCESSION   DATBRK010000228 DATBRK010000000
VERSION     DATBRK010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058823"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1177
                     /locus_tag="VIH70_06960"
     CDS             50..1177
                     /locus_tag="VIH70_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY8116136.1"
                     /translation="MRLSYSSINTYETCPAKFKFQYEDRVPQARSPALSFGDSLHRAL
                     YLFHNRPVPVPPSVGELLDMLEASWVSEGFGDASEEQTYRDHGRQVLSLYHRENADAY
                     RIPAALEFRFTIQVEGVALSGVIDRMDRIPGGGYEIVDYKTNRRLPPQSRIDQDLQLS
                     MYHMAAREIWGIEPERLTLYYLLPGQRMTTVRTPADVDELRRRISVVAERIAAGKFEA
                     RQNPLCDWCEYQALCPLFRHRYEKEQGDPTPRVTEMVDEWIRLKRETRAAYRRFDELV
                     PLINAFADEHGYRRLFGSDGAAIDRRAQHVTAPIEEKVREILEPLGLWDQVLSVDPTK
                     LNALIESRSLSPDVEDALLASREEVRMQHAFYLREAERARR"
     gene            complement(1178..2074)
                     /locus_tag="VIH70_06965"
     CDS             complement(1178..2074)
                     /locus_tag="VIH70_06965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY8116137.1"
                     /translation="MIEAFEAEPGRLDAVVSAHLRVTRAEVQRAIAAGSVLVDGFPRE
                     KSHRLAGGERIEVDLAPSAEVPAEGPPLDLAWRDEHLLVVRKPAGLPTHPTENRLGGT
                     VVNRLLGMGVPLATRGGPLRPGIVHRLDAGTSGLLVVASDDETFEALAATFKRHEAGR
                     AYLALVRGSVPHDRFEVDAPLGRAGGRIRVDATGGRDATTAFEVRERFDRATLLEAEP
                     RTGRTHQIRVHLSSIGHPILGDRRYGGGGEDARGLGLERPFLHAWRIAFDHPVTGERI
                     ELEDPLPPDLERALERAMSGSV"
     gene            complement(2071..2553)
                     /gene="lspA"
                     /locus_tag="VIH70_06970"
     CDS             complement(2071..2553)
                     /gene="lspA"
                     /locus_tag="VIH70_06970"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY8116138.1"
                     /translation="MSAARRSAILLYAVAAGALLLDQLSKAWVEHALSGRPPIRVIPG
                     VLSLTYTTNSGGAFGFGESAPWLFAAATIVVSAVIVVVSTRPVRPWIAVSLGLILGGA
                     LGNLADRVANGPGFGGRVTDFIDLQVWPIFNLADTAIVLGAAILAIASFDGADDHAQP
                     "
BASE COUNT          404 a          991 c          908 g          361 t
ORIGIN      
        1 gttccgtggc cgtacactcg ggcgctgacc tagaggcccg gagggctaga tgcgcctctc
       61 gtactcgagc atcaacacct acgaaacctg ccccgccaag ttcaagttcc agtacgaaga
      121 ccgcgtgccg caggcgcgtt ccccggcgct ctcgttcggt gactcgctcc accgggccct
      181 gtacctgttc cacaaccggc cggtgccggt cccgccctcg gtcggcgagc tcctcgacat
      241 gctcgaggcg tcctgggtct cggaggggtt cggcgacgcc tcggaggagc agacctaccg
      301 cgaccatggt cggcaggtcc tgtcgctgta ccaccgcgag aacgccgacg cgtaccggat
      361 cccggccgcg ctcgagttcc gcttcacgat ccaggtcgag ggcgtcgcgc tgtcgggcgt
      421 gatcgaccgg atggacagga tccccggcgg cggctacgag atcgtcgact acaagaccaa
      481 ccggcgactg ccgccgcagt cgcggatcga ccaggatctc cagctctcga tgtaccacat
      541 ggccgcccgc gagatctggg gcatcgagcc cgaacgcctc accctgtact acctgctccc
      601 cggccagcgg atgacgacgg tccgaacgcc ggccgacgtc gacgagctcc gccggcggat
      661 ctcggtggtc gcggagcgga tcgcggccgg gaagttcgag gcgcgccaga acccgctctg
      721 cgactggtgc gagtaccagg ccctgtgtcc gctcttccgt catcggtacg agaaggagca
      781 gggcgacccg accccgcgcg tgaccgagat ggtggacgag tggatcaggc tgaagcgtga
      841 gacccgcgcg gcgtaccgac ggttcgacga gctggtcccg ctgatcaacg cgttcgccga
      901 cgagcacggg taccggcgcc tcttcgggtc cgacggcgcg gcgatcgacc gccgagcgca
      961 gcacgtgacc gcaccgatcg aggagaaggt acgcgagatc ctcgagccgc tcgggctgtg
     1021 ggaccaggtg ctctcggtcg acccgacgaa gctcaacgcc ctgatcgagt cgcggtcgct
     1081 ctcccccgac gtcgaggacg cgctgctcgc ctcgcgcgag gaggtccgga tgcagcacgc
     1141 gttctacctc cgcgaggccg agcgcgcacg gcgctgatca gaccgagccg ctcatcgccc
     1201 gctcgagcgc gcgctccagg tcgggcggca gcgggtcctc cagttcgatc cgctcgcccg
     1261 tgaccgggtg gtcgaacgcg atccgccacg cgtgcaggaa cggacgctcg agcccgagcc
     1321 cgcgcgcgtc ctcccctccc ccgccgtagc ggcggtcccc gaggatcggg tgcccgatcg
     1381 atgacaggtg gacgcggatc tggtgcgtcc gaccggtgcg cggctcggcc tcgagcagtg
     1441 tcgcccggtc gaaccgctcg cgcacctcga acgccgtcgt cgcgtcgcgc ccgcccgtcg
     1501 cgtccacgcg gatcctcccg cccgcccggc cgaggggcgc gtccacctcg aaccggtcgt
     1561 gggggaccga accgcgcacg agcgcgaggt aggcgcggcc ggcctcgtgg cgcttgaacg
     1621 tcgcggcgag ggcctcgaac gtctcgtcgt cgctcgccac gaccagcagc ccactcgtcc
     1681 ccgcatcgag ccgatgcacg atccccggac gcagcgggcc gccgcgcgtc gccagcggga
     1741 cccccatgcc cagcaggcgg ttgaccacgg tcccgcctag gcggttctcg gtcggatggg
     1801 tcggcagacc ggcgggcttg cgcacgacga gcaggtgctc gtcccgccac gcgagatcca
     1861 gcggcggccc ctccgccggg acctccgccg acggcgccag atccacctcg atccgctcgc
     1921 cgcccgcgag ccggtgcgac ttctcgcgcg gaaacccgtc cacgagcacg gaaccggcgg
     1981 cgatcgcgcg ctgcacctcc gcccgggtca cgcggaggtg ggccgacacc accgcgtcca
     2041 ggcggccggg ttccgcctcg aacgcctcga tcacggctgg gcgtggtcgt cggcgccgtc
     2101 gaagctcgcg atcgccagga tcgccgcccc gagcacgatg gccgtgtcgg cgaggttgaa
     2161 gatcggccag acctgcaggt cgatgaagtc ggtcacccgt ccaccgaacc cgggtccgtt
     2221 cgcgacccgg tcggcgaggt tccccagtgc accgccgagg atcaggccga gggaaacggc
     2281 gatccacggc cggaccgggc gtgtcgagac caccacgatc accgcggaga ccacgatcgt
     2341 ggcggccgcg aacaaccacg gcgcgctctc accgaacccg aacgcgcccc ccgaattcgt
     2401 ggtgtacgtg agcgacagga cgccggggat cacccggatc ggtggccttc cggagagcgc
     2461 gtgttcgacc caggccttgc tcagctgatc caggagcagc gcccccgcgg cgaccgcgta
     2521 caacaggatc gccgaacgcc gagcggcgct cacccgccta ccgccgggac tgggcttgcg
     2581 cgtccttgat gcagagatcg gcgtacggca gggccttcag gcgggccttc tcgatgggct
     2641 tgccacagcg cgagcagatc ccgt
//
LOCUS       DATBRK010000229        14208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1626473, whole genome shotgun sequence.
ACCESSION   DATBRK010000229 DATBRK010000000
VERSION     DATBRK010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626473"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VIH70_06975"
     CDS             <1..986
                     /locus_tag="VIH70_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8116139.1"
                     /translation="ALVGSEDSLLLPTITHIHMSVIPVLAGSGVIFLDGRAHKTIYDG
                     ASAARGHGATIRRFRHNDAEHLEKLVKASRTTPRVIAVDGVNSMTGNAPDVKEFARIA
                     REHDALLYVDDAHGFGVLGERSPDELCEWGLRGNGVVRHQGETYDNIVLVAGFSKAYS
                     SLLSFLALPTRLKDVLKVAAPPYLYSGPSPIASLATTIAGLAVNDARGDLYRLEIYRK
                     TRRVVERLHELDIYTPNESGFPLIEIPLAHRDDIDVVGRYLFDRGVYVTMAAYPLVPK
                     DETGFRIQITAANPDDQIDLLCEVLGEISERFELQHEQHRRLMLRAREARG"
     gene            990..1685
                     /locus_tag="VIH70_06980"
     CDS             990..1685
                     /locus_tag="VIH70_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8116140.1"
                     /translation="MAKGPVAHGLGRAEALSDAGEYAAALQELEAALEAEPHNPAAFT
                     ARGWALENLGPNRLGEARLAYEAALTLDPSAMWAKEGLSNVLRRMGYEREADRLCVEV
                     VEEGRLRYETEEDLWELVGWCELRLGRYQDAVATLREALRRDGRWIAVRFDLALALLC
                     AGERSRAFLEYSRGVHQAVNGGVERLRGCLRVALDDLEETLSERPALATTREAIAARD
                     LLRSTLRTNSQAG"
     gene            1690..3102
                     /locus_tag="VIH70_06985"
     CDS             1690..3102
                     /locus_tag="VIH70_06985"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8116141.1"
                     /translation="MSENRDGRTFIDRRMRILTPLRHRDFRMLWTGMTASLFGDGIFY
                     VALAWQAYELSNTPSALSMIGVAMTVPHVVFLLAGGVVSDRFDRRRVMIAADVTRAVA
                     VGAMGALALSGRLELWHMYVLSGLFGAGTAFFGPAFDAIVPGLVPDAELNPANSLDQL
                     VRPAALRMLGPALGGWIVATGGAGAAFLVDASTFAFSISCVALMHARRATPDAPHGSA
                     IADIREGFAFVRSQVWLWGTFLAATLGYLIFWGPSEVLLPFVVRNDMHGSAADLGLIF
                     AVGGIGAMTSAIVMGNREMPRRNVTFIYVVWTISTLAIAGYGLARFPWQAMAACFAFN
                     ALEGAGTIVWATTKGRLVPARLLGRVSSFDWFISIALVPISYAFTGPVAVAFGAQETL
                     VGAALLGGGITLAFLFLPGMRDVERSGVLVGTRIDASGDTGFGALEGPLAPISAGQGP
                     FPSSGTSGAALRSPVRPTDI"
     gene            3110..6274
                     /locus_tag="VIH70_06990"
     CDS             3110..6274
                     /locus_tag="VIH70_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8116142.1"
                     /translation="MKSRAWLLYLVGGIAALLGFLFLPHLRQGWVFNVISLSSPIAIV
                     LAVRMWKPNAQAAWYLFALGQTFFVIGDVITYNYPDFFGTDLPYPSIGDVAYLLVYPC
                     LVAGLLLLIRRRNPGRDRDSVIDSLIVAIGVGTVQWVFLMSPVAHDSGATLIQKLVGM
                     AYPFMDLILLTAVVRLAMGAGKRAPSMYLMGAAALALFLTDTVYTYISVTGQVYDQSG
                     YLEAGWGVFYLLWGAAALHPSMRHVSDKAPDVERGMSRPRLLLLGAASLIAPGVMLIR
                     LGRADSVDMPVLIGASVMLFVLVVLRMAGLVRRQEQSTLREKALRNAGAALVTATNRE
                     SIYTATLQAARSLVGDDGGLWLLVATDDDVDVFQVVAAAGGAEGAQGGEGTRVSLSIL
                     PQWKRDRLQSHRSYEVPTEDAELANSLGIPENATFFLNAPLFMKEELRGLLVVAGPSS
                     VPRAIRDSIEALSSQVALALDSALTTEILLIRQSEARFSSLVQNSSDVVMVVEADSTI
                     RYMSPSVERVLGHAPGQLEGTKLTVLIHPEDRAGVLQFLTSGGREEDAHPGLTEFRMK
                     HRDDFWLHVETLRTNLMHDENVKGIVLNTRDVSERKLFEEQLQHQAFHDAVTGLANRA
                     LFKDRVEHMIERQARDNLPVSILFMDLDDFKTINDSLGHAAGDRMLSEVGERLKNCLR
                     QADTAARLGGDEFAILLEDGGDGVDAAEVAARILASLEGPFHLEGKEVFGRASIGIAT
                     ADTETGGPEGAEELLRNADVAMYMAKEAGKGRYQVFEPAMHDTALRRLELKADLQRAV
                     DNGEFVLHFQPVIELATGRVEGLEALVRWQHPTRGMIPPLDFIPLAEETGLIVSLGRW
                     VLQEACTQARRLQERYAMDPPLQMAVNLSARQLQRPEIVGEIAETLMQTRLDPSSLVL
                     EITESVMMQDMELSIQRLAELKELGVQLAVDDFGTGYSSLNYIRRFPVDILKVDKSFV
                     DGVNEGGEESALTAAIIELAGILRLRPVAEGIERADQLEKLLALRCDLGQGYYFAKPL
                     PLEEVDELLTTRQTLETRETELSS"
     gene            complement(6320..6835)
                     /locus_tag="VIH70_06995"
     CDS             complement(6320..6835)
                     /locus_tag="VIH70_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8116143.1"
                     /translation="MPSGIREAVPGDARAIAEIHVRSWQATYRGQLTDEYLDGLTVED
                     RVEQHRQSLEEPRAGWTTWVVDEGAGPVGFAVTGPSEDADAGERTAELYAIYLDPERL
                     GTGMGRRLLEHAVEDLRSRGFRAATLWVLETNERARGFYEAAGWTHDGTVTSERVDCE
                     MRPTVRYRVEL"
     gene            complement(6839..8443)
                     /gene="cimA"
                     /locus_tag="VIH70_07000"
     CDS             complement(6839..8443)
                     /gene="cimA"
                     /locus_tag="VIH70_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEY8116144.1"
                     /translation="MSDPLRVELYDTTLRDGAQGTGFSYSIEDRLRILHKLDQLGVPF
                     VEGGWPGANPRETEFFRLATSETLKHATLTAFGMTRKAGETPDRSAVLQDLLRAGTEV
                     VCLVGKAWDLHVTEALRTDLEEGAAMVRDSIAFLRGQGRRVFFDAEHFFDGYRSDPAF
                     ALLVLASAEEAGAERLVLCDTNGGMLPHEVARVVAEVREAVRTPLGIHVHNDAGCAVA
                     NSLIAVEHGAFQVQGVVNGYGERTGNADLIPIAANLVLKMGAECLPDGSLERLTEVAH
                     YVAEVANVAPDSRQPYAGRYAFTHKAGLHASGVERVSRAYEHVEPSTVGNRRGVVASD
                     LGGAATIRMKAEEFGLELGAEGGGRLLLDLKDREARGYTFEVADASLELLMRRADGWR
                     QPFFEIDSYRVHVEERVGDDEPPLAEATVKVVTKGTRHIESAEGQGPVGALDNALRKA
                     LANDYPELEHISLEDFRVRVLDETSGTGAVVRVLIDTSNGQREWTTVGVSENIIEASW
                     EALVDGYLYGLLHPRDDETPSPPTVP"
     gene            complement(8440..9492)
                     /locus_tag="VIH70_07005"
     CDS             complement(8440..9492)
                     /locus_tag="VIH70_07005"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY8116145.1"
                     /translation="MSSAIHTVGVIRGDGTGPEVVAEALKVLDTLDVGVELVEFDLGA
                     ERYLRTGEVLPDEELGRLRTMDAVLLGAVGDPRVRPGVLERDLLLRIRFELDQYVNLR
                     PIVRYPGVETLVPSHGEDDVNMVVVRENSEGLYTGAGGFHRRGTPHEVAVQESINTRH
                     GVERIVRYGFELAMRPGRRRKVTLVHKINVLTYAGDLYQRVVDEVGAEFPDVERDYVH
                     VDAACIYLLDQPSRFDVIVTDNMFGDIITDLGAMIQGGMGVAAGGNINPAGTSMFEPI
                     GGTAPDHAGMGTINPIAAIGALQMLLAQLGEAAAAERVIAGIRFACSKMSSMRAGEMG
                     FSTGEVGDLVVEGASA"
     gene            complement(9489..11102)
                     /locus_tag="VIH70_07010"
     CDS             complement(9489..11102)
                     /locus_tag="VIH70_07010"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY8116146.1"
                     /translation="MANGRDPDVVRIFDTTLRDGEQAPGIALTRAEKVEIAEQLARLE
                     VDVIEAGFPVSSEGEFEAVSEIASSVRGPVIAALARVVPGDITRAAEALGGAERWRIH
                     TFISTSDIHMTDMLRMSNGQVLDAIREGVKLAVGYTEDVEFSAQDATRTELDFLLECF
                     RVAVDAGATTINVPDTVGYAMPHEFGELVRTVREAVPDHVVISTHCHNDLGLAVANSL
                     AGIVNGARQVEVAVNGIGERAGNCSLEEVAMIVRTRREALGVQHGLNLHEIVRTSRLV
                     SMTTGYGVQPNKAVVGSSAFAHESGIHQHGVLANRATYEIMDPLEIGLEGNQLVLGKH
                     SGRHAFADALEKIGIELDEDHLNRAFARFKELADRKIQLTDQDLAAIVSEELGAAAED
                     VFVLEALQVGGGTALSPTASVRLRRGDEMIEESAMGDGMIDAAMGAIQRASGVEGRLV
                     SFNVSAVTGGTDALGDVVVQLDALGRRVTGRGVATDVVEASARAYLAAVNRAMLMANL
                     EADAEQGARGAQGASAKARAVAPGEKVTP"
     gene            complement(11086..12129)
                     /gene="ilvC"
                     /locus_tag="VIH70_07015"
     CDS             complement(11086..12129)
                     /gene="ilvC"
                     /locus_tag="VIH70_07015"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY8116147.1"
                     /translation="MATIYHDAEADPSVLAGQRIAVLGFGSQGHAHAQNLRDSGHDVR
                     VGLREGSGSWSRVEEAGLPVRPTADAVAEADVIMVVLPDTAHGAVYAAEIAPNLKEGD
                     MLMFAHGFSVHFGTVKPPADVDVTMIAPKGPGHLVRRTYEEGIGTPALVAVQQDATGT
                     ALQRALAYGAGIGAARAGIIQTTFKEETETDLFGEQSVLCGGISTLVEAGFDTLVRAG
                     YQPEIAYFECLHELKLIVDLIYEGGLSWMRYSISDTAEWGDYLSGPRIVDEGVRDRMR
                     QVLAEIQDGTFAQEWLGEAGAGFPHFLALRREARGSELERVGRELRPMMPWLHSEEKR
                     VGGTEGDPDGERP"
     gene            complement(12175..12729)
                     /gene="ilvN"
                     /locus_tag="VIH70_07020"
     CDS             complement(12175..12729)
                     /gene="ilvN"
                     /locus_tag="VIH70_07020"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY8116148.1"
                     /translation="MSLHTISVLVEDKPGVLTRVAGLFAARGFNIDSLAVGPTNDAGL
                     SRMTIVVQVDTKPLEQVTKQLNKLVNVIKILEHEAGEAVERELMLVKVKGEGEARARI
                     LEICDVFRVRVLDVTHRTITIEGTGTPIKLEALLELLRDFGIVELSRTGRIALARGDR
                     GIKERSARLRAVAGGEQEWTGGGV"
     gene            complement(12726..>14208)
                     /gene="ilvB"
                     /locus_tag="VIH70_07025"
     CDS             complement(12726..>14208)
                     /gene="ilvB"
                     /locus_tag="VIH70_07025"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY8116149.1"
                     /translation="DSVPMVAITGQVPTPVVGNDAFQEADITGITMPITKHNFLVKDA
                     NELAVTIREAFHIARTGRPGPVLVDIPKDVQLQEFTWSWPGSVDLPGYRPTTKGNLKQ
                     VRSAATMIMESERPVLYVGGGVIKAEAHEELLKLANDGRLPVTTTLMARGAFPDTHEL
                     ALGMPGMHGGYTAVTAMQKADLLVALGSRFDDRVTGQLSSFARGAKIIHVDVDPAEIG
                     KNRAADVPIVGDCKDVIANLHTELRRLQDEAGGVPDRRAWLAQLDEWKRTYPYRYDQP
                     ADGPLKPQFCIERLYEHTKGDAIVVAGVGQHQMWASQFWRFSEPRTWVNSGGLGTMGF
                     AVPAALGAKAGRPDRRVIAIDGDGCFQMTSQELATSTAERIPFVTAIINNAHLGMVRQ
                     WQELFYEERYSQVYLSYDVPDYVKLAEAYGCVGLRAEHPDEVDAVIDKALSIEDRSVV
                     IDFRVDSKEMCFPMVPAGASNDDIIVGPEGLRPTNDPAEAPVA"
BASE COUNT         2031 a         5102 c         4774 g         2301 t
ORIGIN      
        1 cggcgctggt cgggtccgag gactcgctgc tccttccgac gatcacccac atccacatgt
       61 ccgtgatccc cgtcctggcc ggcagcggcg tgatcttcct cgacggccga gcgcacaaga
      121 cgatctacga cggcgcgagc gccgcccgcg ggcacggggc cacgatccgt cgcttccgcc
      181 acaacgacgc cgagcacctc gagaagctcg tcaaggcctc gaggacgacg ccccgcgtga
      241 tcgcggtcga cggggtgaac tcgatgaccg ggaacgcccc ggacgtcaag gagttcgcgc
      301 gcatcgcccg agagcacgac gcgctcctgt acgtcgacga cgcccacggg ttcggcgtgc
      361 tcggggagcg ctcgccggac gagctctgcg agtggggcct gcgcggcaac ggcgtcgtcc
      421 gacaccaggg ggagacctac gacaacatcg tcctggtcgc ggggttcagc aaggcgtaca
      481 gctcgctgct gtccttcctc gcgctgccga cgcgcctcaa ggacgtgctc aaggtcgcgg
      541 cccccccgta cctgtattcg gggccctcgc cgatcgcctc gctcgcgaca acgatcgccg
      601 gtctcgcggt gaacgacgcg cggggcgacc tgtaccgcct cgagatctac cgcaagaccc
      661 ggagggtcgt ggagcggctg cacgagctcg acatctacac gcccaacgag tccgggttcc
      721 cgctcatcga gatccctctg gcgcaccgcg acgacatcga cgtggtcggc cgctacctgt
      781 tcgatcgcgg cgtctacgtg acgatggcgg cgtaccccct ggtcccgaag gacgagacgg
      841 gcttccgcat ccagatcacc gcggcgaacc cggacgacca gatcgacctc ctgtgcgagg
      901 tgctcggtga gatcagcgag cggttcgagc tccagcacga acagcaccgg cgtctgatgc
      961 tccgtgcccg agaggcgagg gggtaggcgg tggccaaggg tcccgtcgca cacgggctcg
     1021 gccgcgccga ggcgctgtcc gacgcgggcg agtacgcggc cgcgctccag gagctggagg
     1081 ccgcgctcga ggccgagccg cacaaccccg ccgcattcac ggcccgcggc tgggcgctcg
     1141 agaacctcgg tcccaaccgg ctcggcgagg cgcggctggc ctacgaggcc gcgctcacgc
     1201 tcgacccttc ggcgatgtgg gccaaggagg ggctctcgaa cgtgctgcgg cgcatgggct
     1261 acgaacgtga ggccgatcgg ctctgcgtgg aggtcgtgga ggaagggcgt ctgcgctacg
     1321 aaacggagga agacctgtgg gagctcgtcg gatggtgcga gctccggctc ggccggtacc
     1381 aggacgcggt ggcgacgctc cgtgaggccc tgcgccgaga tggtcgctgg atcgcggtac
     1441 ggttcgacct cgccctggcc ctgctctgcg cgggcgaacg atcgcgcgcc ttcctcgagt
     1501 attcgcgcgg ggtccaccag gcggtgaacg gcggcgtcga acggctgcgc ggctgcctgc
     1561 gcgtcgcgct ggacgacctg gaggagacgc tctccgagcg cccggcgctc gccacgaccc
     1621 gcgaggcgat cgcggcacgc gacctccttc ggtccaccct gcggaccaac tcccaagccg
     1681 gctgagcgga tgagcgagaa tcgggacggt cgcacgttca tcgatcggcg gatgcggatc
     1741 ctcacgccgc tgcgacatcg cgacttccgg atgctctgga cggggatgac ggcctccctg
     1801 ttcggcgacg ggatcttcta cgtggcgctc gcctggcagg cgtacgagct ctcgaacacg
     1861 ccgagcgccc tgtccatgat cggcgtcgcg atgacggtcc cgcacgtcgt gttcctgctg
     1921 gcggggggcg tggtctccga tcggttcgac cgacggaggg tgatgatcgc ggccgacgtg
     1981 acgcgcgccg tggcggtcgg tgcgatgggt gccctcgcgc tctcgggccg gctcgagctc
     2041 tggcacatgt atgtgctctc gggtctgttc ggcgccggga cggcgttctt cggtccggcg
     2101 ttcgatgcga tcgttcccgg gctcgtcccg gacgccgagc tgaacccggc gaactcgctc
     2161 gaccagctcg ttcgacccgc ggccctgcgg atgctcggac cggcgctggg cgggtggatc
     2221 gtggcgacgg gtggcgccgg ggccgccttc ctcgtggacg ccagcacgtt cgccttctcg
     2281 atctcgtgcg tggcgctcat gcatgcccgc cgagcgaccc ccgacgcgcc ccatgggtcg
     2341 gcgatcgccg acatccgcga ggggttcgcg ttcgtccgat cgcaggtctg gctgtggggc
     2401 accttcctcg cggcgacgct cggctacctg atcttctggg gaccttcgga ggtgctgctc
     2461 ccgttcgtcg tccgaaacga catgcacggg tcggccgcgg acctcggtct gatcttcgcc
     2521 gttgggggga tcggggccat gacctcggcg atcgtgatgg gcaaccggga gatgccgcgc
     2581 aggaacgtga cgttcatcta cgtcgtgtgg acgatctcga cgctcgcgat cgccggatac
     2641 ggtctcgcgc ggttcccgtg gcaggcgatg gcggcctgct tcgcgttcaa tgcgctcgag
     2701 ggcgcgggga cgatcgtctg ggcgacgacc aagggccgcc tcgtgccggc gcggctgctc
     2761 gggcgcgtct cgagcttcga ctggttcatc tcgatcgcgc tcgtgccaat ctcctacgcg
     2821 ttcacgggtc cggtcgccgt ggcgttcggc gcccaggaga cgctggtcgg agccgccctg
     2881 ctgggcgggg ggatcaccct ggccttcctg ttcctgccgg ggatgcgcga tgtcgagcgg
     2941 agcggcgtcc tcgtgggcac gaggatcgac gcgagcgggg acacggggtt cggggccctc
     3001 gagggacccc tggcgcccat ctccgctggt caggggccct ttccgtcttc gggaacttcc
     3061 ggggcggctc taaggtcgcc cgttcggccc accgatatct gaggcagaga tgaagtcccg
     3121 ggcttggctc ctgtacctag tcggcggcat cgccgcgctg ctcggtttcc tgttcctgcc
     3181 gcaccttcga cagggatggg tcttcaacgt gatctctctc tcatcgccga tcgcgatcgt
     3241 cctcgcggtc cgcatgtgga aaccgaacgc ccaggccgcc tggtatctgt tcgcgctcgg
     3301 tcagacgttc ttcgtcatcg gggacgtcat cacctacaac taccccgact tcttcggcac
     3361 cgatctcccc tacccgtcga tcggcgacgt ggcctatctc ctggtctacc cgtgcctggt
     3421 tgcaggcctg ctcctgctga tccgcagacg caacccgggc cgggaccggg acagcgtcat
     3481 cgattcgctc atcgttgcga tcggcgtcgg cacggtgcag tgggtgttcc tgatgtcccc
     3541 ggtggctcat gactccggcg caaccctgat ccagaagctc gtggggatgg cctacccgtt
     3601 catggacctc atcttgctga ccgccgtcgt ccgtctcgcg atgggcgcgg gcaagcgggc
     3661 cccctcgatg tacctgatgg gggcggccgc cctcgccctg ttcctcaccg acaccgtgta
     3721 cacgtacatc tccgtcaccg gccaggtgta cgaccagtcc gggtacctcg aggcgggatg
     3781 gggcgtcttc tatctgttgt ggggcgcggc cgcactgcac ccctcgatgc ggcacgtctc
     3841 ggacaaggcc cccgacgtcg aacgcgggat gtccaggccc cgactgctgt tgctcggcgc
     3901 cgcgtcgctg atcgcaccgg gcgtcatgct gatccggctc ggccgggcgg actccgtgga
     3961 catgcccgtc ctgatcgggg cgtcggtcat gttgttcgtc ctggtcgtcc tccgcatggc
     4021 gggactggtc cggcgccagg aacagtccac gctccgcgag aaggcccttc gcaacgccgg
     4081 tgccgcgctc gtcacggcga cgaatcggga gagcatctat acggccacgt tgcaggcggc
     4141 gcgctcgctc gtgggcgacg acggcggcct gtggctgttg gtcgccaccg acgacgacgt
     4201 ggatgtgttc caggtggtgg cggcggccgg aggggccgag ggggctcagg ggggcgaggg
     4261 cacgagggtc tccctgtcga tccttcctca gtggaagcgc gaccggctcc agtcccaccg
     4321 ctcgtacgag gtccccaccg aggacgccga gctcgcgaac tcgctcggca tacccgagaa
     4381 cgcgaccttc ttcctgaacg ccccgctgtt catgaaagag gagctccggg ggttgctcgt
     4441 ggtcgccgga ccctcgagcg tcccccgggc gatccgcgac tcgatcgagg cgctctcctc
     4501 ccaggtcgcc ctggcgctcg acagcgccct gacgaccgag atcctgctga tccgccagag
     4561 cgaggcccgg ttctcctcgc tggtccagaa ctcgtccgac gtggtcatgg tcgtcgaggc
     4621 cgactccacg atccggtaca tgagcccgtc ggtggagcgc gtgctcgggc acgcgccggg
     4681 ccagctcgaa ggcacgaagc tcacggtcct gatccacccc gaggaccgag ccggcgtgct
     4741 gcagttcctc acctcgggcg gacgcgagga agacgcgcat ccgggcctca ccgagttccg
     4801 gatgaaacat cgggacgact tctggctgca cgtcgagacc ctgcggacga acctgatgca
     4861 cgacgagaac gtgaagggca tcgtcctgaa cacgcgtgac gtctccgaac gcaagctgtt
     4921 cgaggagcag ctgcagcacc aggcgttcca cgacgcggtc acggggctcg cgaaccgggc
     4981 gctgttcaag gaccgcgtcg aacacatgat cgagcggcag gcgcgcgaca acctcccggt
     5041 ctcgatcctg ttcatggacc tggacgactt caagaccatc aacgactcgc tcggccatgc
     5101 ggcgggcgac cggatgctta gcgaggtcgg cgagcgactc aagaactgcc ttcgacaggc
     5161 ggacaccgcg gctcggctcg gcggcgacga attcgcgatc ctgctcgagg atggggggga
     5221 cggggtcgac gcggccgagg tcgccgcgag gatccttgcg tcgctcgagg gtccgttcca
     5281 cctcgagggc aaggaggtct tcgggcgagc ctcgatcggc atcgcgaccg ccgatacgga
     5341 gaccggcggc cccgagggcg cggaggagct gctccggaac gccgacgtcg cgatgtacat
     5401 ggccaaggaa gccggcaagg gtcgctacca ggttttcgaa cccgcgatgc acgacaccgc
     5461 gctccggcga cttgagctca aggcggatct gcagcgcgcc gtcgacaacg gcgagttcgt
     5521 cctgcacttc caaccggtga tcgagctcgc gaccgggcgg gtggagggtc tcgaggcgct
     5581 cgtccgctgg cagcatccca cgcgcggcat gatcccaccc ttggacttca tcccactggc
     5641 ggaggaaacc gggttgatcg tgtcgctcgg gcggtgggtg ctgcaggagg cgtgcacgca
     5701 ggctcgccgg ctgcaggagc ggtacgcgat ggacccgccg ctgcagatgg cggtcaacct
     5761 ctccgcgcga cagctccagc gcccggagat cgtcggcgag atcgccgaga ccttgatgca
     5821 gacccggctc gacccgagca gcctggtctt ggagatcacc gagtccgtga tgatgcagga
     5881 catggagctg tcgatccagc gcctggcgga actcaaggag ctcggcgtcc agctggcggt
     5941 cgacgacttc ggcaccggat actcgagcct gaactacatc cgccggttcc cggtcgacat
     6001 cttgaaggtg gacaagtcct tcgtcgacgg cgtcaatgag ggcggcgagg aatcggcgct
     6061 cacggccgcc atcatcgagc tcgcggggat cctgcgcctg cggccggtgg ccgagggcat
     6121 cgaacgggcc gaccagctcg agaagctcct cgccctgcga tgcgacctgg gccaggggta
     6181 ctacttcgcc aaaccgctcc ccctcgaaga ggtggacgag ctcctcacca cgcgccagac
     6241 gctcgagacc cgggagaccg agctctcctc ctgagcgcgc gaggtgggcc ctgccggacg
     6301 gtgcgggcgg ggtgagcggc tacagctcga cgcggtaccg aaccgtcgga cgcatctcgc
     6361 aatcgacccg ctcgctcgtc acggtcccgt cgtgcgtcca tccggcggcc tcgtagaagc
     6421 cccgagcgcg ctcgttcgtc tcgagcaccc acagggtggc cgcccggaac ccccgagacc
     6481 gcagatcctc gacggcgtgc tcgagcagcc gtcgtcccat tccggtcccg agccgctccg
     6541 ggtccaggta gatcgcgtag agctcggccg tccgctcgcc ggcgtccgca tcctcgctcg
     6601 gaccggtgac cgcgaacccg acggggcccg cgccctcgtc caccacccag gtggtccagc
     6661 ccgcgcgcgg ctcctccaaa ctctggcgat gctgctccac gcgatcctcg acggtgaggc
     6721 cgtcgagata ctcgtcggtc agctgccccc gatacgtggc ctgccacgat cgaacgtgga
     6781 tctcagcgat cgcccgcgcg tccccgggaa cggcctcgcg gatccctgac ggcatacctc
     6841 agggtaccgt cggcggcgat ggcgtctcgt cgtcccgcgg gtggagcagc ccgtacaggt
     6901 acccgtcgac cagcgcctcc cacgatgcct cgatgatgtt ctccgagacg ccgaccgtgg
     6961 tccactcccg ctggccgttc gacgtgtcga tcaggacgcg caccacggcg cccgtcccgg
     7021 acgtctcgtc cagcacgcgt acccggaagt cctcgagcga gatgtgctcg agctcggggt
     7081 agtcgttcgc gagcgccttg cggagcgcgt tgtcgagcgc gccgaccggc ccctggccct
     7141 ccgcgctctc gatgtgccgg gtccccttcg tgacgacctt gaccgtagcc tcggcgagcg
     7201 gcggctcgtc gtcgccgacg cgttcctcga cgtgaacccg atagctgtcg atctcgaaga
     7261 agggttgccg ccagccgtcg gcccggcgca tcagcaactc gagggaggcg tcggcgacct
     7321 cgaacgtgta cccgcgtgcc tcgcgatcct tcagatccag cagcagacgg ccgccaccct
     7381 cggcgccgag ctcgaggccg aactcctcgg ccttcatccg gatcgtcgcc gctcccccga
     7441 ggtcgctcgc cacgacgccg cggcggttgc cgaccgtcga gggttccacg tgctcgtacg
     7501 cccgggacac gcgctcgacg ccgctcgcat gcaggccggc cttgtgagtg aaggcgtacc
     7561 ggcccgcgta cggctgtcgc gagtcggggg cgacgttcgc cacctcggcc acgtagtgcg
     7621 ccacctcggt gagtcgctcc agcgacccgt cgggcaggca ctcggcgccc atcttcagga
     7681 cgaggttcgc ggcgatcggg atcaggtcgg cgttgccggt gcgttcgccg tagccgttca
     7741 cgacaccttg gacctggaag gccccgtgct ccacggcgat gagcgagttc gcgacggcgc
     7801 atcccgcgtc gttgtggacg tggatcccga gcggggtgcg caccgcctcg cggacctcgg
     7861 ccacgacccg cgccacctcg tgtggcagca tgcccccgtt cgtgtcgcac aggaccaatc
     7921 gctcggcccc ggcctcctcg gccgaagcca ggacgaggag cgcgaacgcg ggatcgctcc
     7981 ggtagccgtc gaagaagtgc tcggcgtcga agaacacccg gcgcccctgt ccacgcagga
     8041 acgcgatcga gtcgcggacc atggccgcgc cctcctccag atccgtccgg agcgcctcgg
     8101 tcacgtggag gtcccacgcc ttgcccacca ggcagaccac ctcggtcccc gcccggagca
     8161 ggtcctggag caccgcgctc cgatccggcg tctcccccgc cttgcgggtc atcccgaagg
     8221 ccgtgagcgt cgcgtgcttg agcgtttcgc tcgtcgcgag ccggaagaac tcggtctctc
     8281 gcgggttcgc cccgggccag ccgccctcca cgaacgggac gccgagctgg tcgagcttgt
     8341 ggaggatccg gagcctgtcc tcgatcgagt acgagaagcc ggtcccctgc gcgccgtcgc
     8401 gcagggtggt gtcgtacagc tcgacgcgga gcggatcgct cacgcggagg ccccctcgac
     8461 gacgaggtcg ccgacctctc cggtcgaaaa gcccatctcg ccggcgcgca tcgaggacat
     8521 cttcgagcac gcgaaccgga tgccggcgat cacgcgctct gccgcggcgg cctcgccgag
     8581 ttgcgcaagc agcatctgca gcgcaccgat cgccgcgatc gggttgatcg tccccatgcc
     8641 ggcgtgatcg ggcgccgtgc cgccgatagg ctcgaacatg ctcgtcccgg ccgggttgat
     8701 gttgcctccc gccgcgacgc ccatccctcc ctggatcatc gcgcccaggt ccgtgatgat
     8761 gtcgccgaac atgttgtcgg tgacgatcac gtcgaaccgc gagggctgat cgagcaggta
     8821 gatgcacgcg gcgtccacgt ggacgtagtc ccgctcgacg tcggggaact ccgccccgac
     8881 ctcgtcgacc acgcgctggt agaggtctcc ggcgtaggtg agcacgttga tcttgtgcac
     8941 cagcgtcacc ttgcgcctgc gtcccgggcg catcgcgagc tcgaacccgt accggacgat
     9001 ccgctcgacg ccgtgccggg tgttgatcga ctcctgcacg gcgacctcgt ggggcgtgcc
     9061 cctgcgatgg aagcccccgg cgcccgtgta caggccctcg gagttctcgc ggacgacgac
     9121 catgttcacg tcgtcctcgc cgtgcgaggg cacgagggtc tccacgccgg ggtaccgcac
     9181 gatcggtcgg aggttcacgt actggtcgag ctcgaaccgg attcgcagca gcaggtcgcg
     9241 ttcgaggacg ccggggcgta cgcgcgggtc acccacggcg ccgagcagca cggcgtccat
     9301 cgtgcgcagg cgcccgagct cctcgtccgg cagcacctcg ccggtgcgca ggtaccgttc
     9361 ggcgcccagg tcgaactcga cgagctcgac cccgacgtcg agcgtgtcca gcaccttcag
     9421 ggcctcggcg acgacctcgg gaccggtccc gtccccccgg atcaccccga ccgtgtggat
     9481 cgcggagctc acggcgtgac cttctccccg ggggccaccg ctcgcgcctt cgcgctcgct
     9541 ccttgagccc cccgagcccc ctgctccgca tcggcctcga ggttcgccat cagcatcgcc
     9601 cggttgaccg cggcgaggta ggcccgcgcg ctcgcctcga ccacgtcggt cgcgaccccc
     9661 cggcccgtca cccggcgacc gagcgcgtcg agctgcacga ccacgtcacc taacgcgtcc
     9721 gtcccgccgg tgaccgccga gacgttgaac gagaccagcc ggccctcgac cccggacgcc
     9781 cgctggatcg cgcccatcgc cgcgtcgatc atgccgtcgc ccatcgcgga ctcctcgatc
     9841 atctcgtcgc ctcgccggag ccggacgctc gcggtcggcg acagcgcggt gccacccccc
     9901 acctggagcg cctcgagcac gaacacgtcc tcggcggccg ccccgagctc ctccgagacg
     9961 atcgccgcga gatcctggtc cgtgagctgg atcttgcggt ccgcgagctc cttgaagcgc
    10021 gcgaacgcgc ggttgaggtg gtcctcgtcg agctcgatcc cgatcttctc gagcgcatcg
    10081 gcgaacgcgt gccgtcccga gtgcttgccg aggacgagct ggttgccctc cagaccgatc
    10141 tcgagggggt ccatgatctc gtaggtcgcc cggttcgcga gtacgccgtg ttggtggatc
    10201 ccgctctcgt gcgcgaacgc cgacgagccg accaccgcct tgttcggctg gacgccgtac
    10261 ccggtcgtca tgctaaccag gcgtgacgtg cggacgatct cgtgcaggtt cagcccgtgt
    10321 tggacgccga gcgcctcccg gcgagtccgg acgatcatcg cgacctcctc gagcgagcag
    10381 ttgccggccc gctcgccgat cccgttcacg gccacttcga cctgcctcgc tccgttcacg
    10441 atcccggcca gcgagttcgc caccgcgagc ccgaggtcgt tgtggcaatg cgtcgagatc
    10501 acgacgtggt cggggaccgc ctcccgcacc gtgcggacca gttccccgaa ctcgtgcggc
    10561 atcgcgtacc cgaccgtgtc gggcacgttg atcgtggtgg cgccggcgtc caccgcgacg
    10621 cggaagcact cgagcaggaa gtcgagctcc gtgcgggtgg cgtcctgcgc gctgaactcc
    10681 acgtcctcgg tgtaaccgac ggcgagcttg acgccctcgc ggatcgcgtc gaggacctgc
    10741 ccgttgctca tccggagcat gtccgtcatg tggatatcgc tggtcgagat gaacgtgtgg
    10801 atgcgccagc gctcggcccc gccgagcgcc tcggccgccc gggtgatgtc gccgggcacg
    10861 acgcgggcga gcgccgcgat gaccgggccc ctgacgctgg acgcgatctc gctgaccgcc
    10921 tcgaactcgc cctcgctgga gacggggaag cccgcctcga tcacgtcgac ctcgagacgg
    10981 gccagctgct ccgcgatctc gaccttctcc gcccgggtga gcgcgatccc cggtgcctgc
    11041 tcgccgtcgc gcagcgtggt atcgaagatc cgtactacat cggggtcacg gccgttcgcc
    11101 atctgggtct ccttcagtcc cgcccacccg cttctcctcg gaatgcagcc acggcatcat
    11161 cgggcgcagc tcgcgcccca cgcgctcgag ctcggagccg cgggcctcgc gacgcagcgc
    11221 gaggaagtgg gggaagccgg caccggcctc ccccagccac tcctgcgcga aggtgccgtc
    11281 ctggatctcc gccaggacct ggcgcatgcg gtcgcgaacg ccctcgtcga cgatccgggg
    11341 tccgctcagg tagtcgcccc actcggcggt gtccgagatc gagtatcgca tccacgacag
    11401 gccgccctcg tagatcaggt cgacgatcag cttgagctcg tgcaggcact cgaagtacgc
    11461 gatctctggc tggtagccgg cccgcacgag cgtgtcgaac ccggcctcaa cgagcgtgga
    11521 gatcccgcca cagagcacgg actgctcgcc gaacagatct gtctcggtct cctccttgaa
    11581 ggtggtctgg atgatgcctg cccgggcggc tccgatcccc gcgccgtacg cgagggcgcg
    11641 ctgcagggcc gtgcccgtcg cgtcctgctg gaccgcgacg agcgccggcg tgccgatccc
    11701 ctcctcgtac gtccggcgga ccaggtgacc cggtcccttg ggggcgatca tcgtgacgtc
    11761 cacgtcggcg ggcggcttca cggtcccgaa atgcaccgag aacccgtgcg cgaacatcag
    11821 catgtcgccc tccttcaggt tcggcgcgat ctccgcggcg tacaccgcgc cgtgcgccgt
    11881 atcggggagc accaccatga tcacgtcggc ctccgccacg gcgtcggcgg tcggccgcac
    11941 cgggaggccc gcctcctcga ccctcgacca ggagcccgaa ccctcgcgga gcccgacccg
    12001 gacgtcgtgg cccgaatccc tcaggttctg cgcgtgcgcg tgaccctggc tcccgaagcc
    12061 caggacggcg atccgttgcc ccgcaaggac cgacggatcg gcttccgcgt cgtggtagat
    12121 cgtcgccatc ggtcccgtac cacctttccg gtcacgtgac ggtcggggcg tcggtcagac
    12181 gccgccgccg gtccactcct gctcgccccc ggcgaccgcc cggagccgcg cggaacgttc
    12241 cttgatcccg cggtcacccc tcgcgagcgc gatccgtccg gtgcgcgaga gctcgacgat
    12301 tccgaagtcg cgcaggagct cgagcagcgc ctcgagcttg atcggcgtgc ccgtgccctc
    12361 gatcgtgatc gtccgatggg tcacgtcgag cacccggacg cggaagacat cgcagatctc
    12421 caggatgcgg gcccgcgcct cgccctcgcc cttgaccttc acgagcatca gctcgcgctc
    12481 gaccgcctcg cccgcctcgt gctcgaggat cttgatcacg ttcacgagct tgttgagctg
    12541 cttcgtgact tgctcgagcg gcttggtgtc cacctggacg acgatcgtca tccgcgacag
    12601 gccggcgtcg ttggtcgggc cgacggcgag cgagtcgatg ttgaacccac gcgccgcgaa
    12661 caggccggcg acgcgggtca gcacgcccgg cttgtcctcg accaacaccg agatcgtgtg
    12721 gaggctcacg cgaccggcgc ctcggccggg tcgttcgtcg gccgcaggcc ctcgggcccg
    12781 acgatgatgt cgtcgttgct cgcgccggcc gggaccatcg ggaagcacat ctccttcgaa
    12841 tccacgcgga agtcgatcac gacgctccgg tcctcgatcg agagcgcctt gtcgatcacg
    12901 gcgtccacct cgtcggggtg ctcggcgcga aggcccacgc agccgtaggc ctcggcgagc
    12961 ttcacgtagt ccggcacgtc gtaggacaga tacacctgcg agtaccgctc ctcgtagaac
    13021 agctcctgcc actgccggac catcccaagg tgggcgttgt tgatgatcgc cgtcacgaac
    13081 gggatccgct cggcggtgga ggtcgcgagc tcctggctgg tcatctggaa gcaaccgtcc
    13141 ccgtcgatcg cgatcacgcg gcggtccggc cggccggcct tggcgcccag agccgcgggc
    13201 accgcgaacc ccatcgtccc gagccccccg gagttcaccc aggtccgcgg ctcgctgaac
    13261 cgccagaact gcgacgccca catctggtgc tgccccacgc ccgcgacgac gatcgcgtcg
    13321 cccttcgtgt gctcgtagag ccgctcgatg cagaactgcg gcttgagcgg cccgtcggcc
    13381 ggctggtcgt agcggtaggg gtacgtccgc ttccactcat cgagctgggc gagccatgcc
    13441 ctccgatccg gcaccccgcc cgcctcgtcc tgcagacgtc gcagctcggt gtggaggttc
    13501 gcgatcacgt ccttgcagtc gcccacgatc ggcacgtcgg ccgctcgatt cttgccgatc
    13561 tcggccgggt cgacgtcgac gtggatgatc ttcgcgcccc tggcgaacga ggacagctgg
    13621 ccggtcaccc ggtcgtcgaa ccgcgaaccg agcgccacca gcaggtcggc cttctgcatc
    13681 gccgtcacgg ccgtgtaccc gccgtgcatc cccggcatcc cgagcgcgag ctcgtgcgtg
    13741 tcggggaacg cgccacgcgc catcagcgtg gtcgtgaccg ggagcctccc gtcgttcgcg
    13801 agcttcagca gctcctcgtg ggcctcggcc ttgatcacgc cgccgcccac gtacaggacc
    13861 gggcgctcgg actccatgat catcgtggcg gccgaacgca cctgcttgag gttgcccttg
    13921 gtggtcggcc gatagccggg gaggtcgacg gagccgggcc agctccaggt gaactcctgc
    13981 agctggacgt ccttcgggat gtcgacgagg acgggtcccg gccggccggt ccgggcgatg
    14041 tggaacgcct cgcggatcgt gacggcaagc tcgttcgcgt ccttgaccag gaagttgtgc
    14101 ttggtgatgg gcatcgtgat gccggtgatg tcggcctcct ggaaggcgtc gttgccgacg
    14161 accggcgtcg ggacctgccc ggtgatcgcg accatcggga ccgagtcc
//
LOCUS       DATBRK010000230         4146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1046075, whole genome shotgun sequence.
ACCESSION   DATBRK010000230 DATBRK010000000
VERSION     DATBRK010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046075"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VIH70_07030"
     CDS             <1..247
                     /locus_tag="VIH70_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEY8116150.1"
                     /translation="EAFRAEGCSAPRSWGNGPGDAYGPHAHGFHKVLFCLGGSITFHL
                     DDGDVELGPGDRLDIEPGTEHAATVGPNGCSCLEASR"
     gene            244..1368
                     /locus_tag="VIH70_07035"
     CDS             244..1368
                     /locus_tag="VIH70_07035"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY8116151.1"
                     /translation="MRVAAHVDQLFFTVPGGVGTYIRNLVPALAGRDPSLEVTLFHAR
                     FATARPSEPWMDGFAEAELSGSIRSLYPRWNLTARPALPVAIRSADLVHAMSASAVPP
                     TARGKRLVVTVHDLAFDRYPKLFPRAWRIMYRLGLRAAVRRADAIVTPSRATAEDVLS
                     RTRVDPRKLHVVPEAAALPPSEVDVGEALARLKVRTPYVLFVGTIEPRKNLVRLVRAY
                     RRVAATGVPHALVLAGPLGWHHEALMRELVLQGPGEIVMTGALAPAELDALYRAADAF
                     VYPSVYEGFGLPVLEAMSRGVPVVASTTSALPEITGEAALGVNPRSVREITAAIASLI
                     GDVALAERLASAGRLQAERFSWDETARITLEVYERVLDAR"
     gene            complement(1376..2239)
                     /locus_tag="VIH70_07040"
     CDS             complement(1376..2239)
                     /locus_tag="VIH70_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018549101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY8116152.1"
                     /translation="MRAEDVRTVGVIGCGLMGSGISEVVARAGQTAVVLETSDELVER
                     GRGRIETSTLRAVERGSLDAEERAAVLGRISLTTDVQDLADVDLVIEAATEDHDTKVV
                     MFRRLDEVAKPEVILASNTSSIPIADLGAATSRPDKVLGMHFFNPVPVMGLIELVRAI
                     SSSDDTMEFGRAYGVVLGKTTVESRDRAGFIVNALLIPYLNGAIRMLEDGFATRDDID
                     TAVHLGLNHPMGPLRLIDLIGLDTHLFIANVLFEEFKEPTFAPPPLLRRMVTAGRLGR
                     KVGRGFYDYGS"
     gene            2311..2895
                     /locus_tag="VIH70_07045"
     CDS             2311..2895
                     /locus_tag="VIH70_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116153.1"
                     /translation="MREEPRLRGLPTFGLAMAGLVLGHVVAYLIAVPDPHHRAFVLQE
                     TGHEYLPAMAEAALVLALAGMATVVIRAFAPGARRGSERFSRLAGRLGLVQVLAFGGL
                     EVVERVVVGAPLRHLASDHILVIGTAVQVVVALAGAAFLRWLARTSSKLSETALPRLP
                     LPRAGAVLALASTSWGPGISAFGSVGNVRAPPSS"
     gene            2962..3633
                     /locus_tag="VIH70_07050"
     CDS             2962..3633
                     /locus_tag="VIH70_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116154.1"
                     /translation="MPKRSMRLPSATALVVTLLVLMAAPAFAHEERSVGDFDMAVGFG
                     AEPAYAGEVNSVQLLLSRNGKPVVDLGDTLDVEVTFGDQTQKFPLEPFFEEGEFGTLG
                     DYRAWFIPTRSGRYTFHFSGTIDGQAVDESFTSGPNTFSDVESPQSVEFPVQDPTTGE
                     LAERIDRVEPRLASSIDDVQTEVQATADDASSAKTIGLIGLVVGAIGLIVAIVALVAS
                     RRKRV"
     gene            3630..>4146
                     /locus_tag="VIH70_07055"
     CDS             3630..>4146
                     /locus_tag="VIH70_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8116155.1"
                     /translation="MIGVLAHAGGVPESVSTGLLGLGLVTGWIGLSRLRDRGFPSLPR
                     WGGWALVGLAPVAVVASIAVPSLLWPPPAAIRPRSTATLSFVEPTPNQVVTGSTLDVR
                     LRLDGGRIVQSSSTTLTPDTGHIHLFVDGAIVSMTYGTKQRVPLGGLDPGPHVLQAEF
                     VAADHGPFDPRV"
BASE COUNT          546 a         1488 c         1448 g          664 t
ORIGIN      
        1 cgaggcgttc cgggccgagg ggtgctcggc gccgagatcg tggggcaacg gacccggcga
       61 cgcgtacggc ccgcacgccc acggcttcca caaggtgctg ttctgcctcg gcggctcgat
      121 cacgttccac ctcgacgacg gtgacgtcga gctcgggccg ggcgaccgcc tcgacatcga
      181 gccggggacc gagcacgcgg cgacggtcgg gccgaacggc tgctcgtgcc tggaggcctc
      241 gcgatgaggg tcgccgcaca cgtcgatcag ctcttcttca ccgtgccggg cggcgtgggg
      301 acctacatcc ggaacctggt gcccgcgctc gctgggcgcg atccgtcgct ggaggtgacg
      361 ctcttccacg cgcggttcgc gaccgctcgc ccatcggaac cgtggatgga cggcttcgcc
      421 gaggcggagc tgtcgggctc gatccggagc ctgtacccgc ggtggaacct cacggccaga
      481 cccgcgctgc cggtggcgat ccgctcggcc gacctcgtgc acgcgatgag cgcgtcggcc
      541 gtccccccca ccgcccgggg caagcgcctg gtcgtcaccg tgcacgacct ggcgttcgac
      601 cgctacccga agctgttccc gagggcgtgg cggatcatgt accggctcgg cctgcgcgcg
      661 gccgtccggc gcgcggacgc gatcgtgacg ccgtcgcgcg ccaccgccga ggacgtcctg
      721 tcgcgcacga gggtcgaccc gcgcaagctg cacgtggtcc ccgaggccgc cgcgttgccc
      781 ccgagcgagg tcgacgtggg cgaggcgctg gcgcgcctga aggttcgaac cccctacgtc
      841 ctgttcgtcg gcacgatcga gccgaggaag aacctggtcc ggctggtccg cgcgtaccgg
      901 cgcgtggccg ccaccggcgt gccgcacgcg ctggtgctcg ccggtccgct cggctggcac
      961 cacgaggccc tgatgcggga gctggtgctc caggggccgg gcgagatcgt gatgacgggc
     1021 gccctcgcgc ccgccgagct cgacgcgctt taccgggccg ccgatgcgtt cgtctacccg
     1081 tcggtgtacg agggattcgg cctgccggtg ctcgaggcga tgagccgcgg ggtccccgtc
     1141 gtggcctcga ccacgtccgc gctccccgag atcaccggcg aggcggcgct gggcgtgaac
     1201 ccgcggtcgg tgcgggagat caccgccgca atcgcttcgc tgatcggcga cgtcgccctc
     1261 gccgaacgcc tggcctcggc cgggcggctg caggccgaac gcttctcgtg ggacgagacc
     1321 gcgaggatca cactcgaggt ctacgagcgg gtcctcgacg cgaggtgagc gcgactcagc
     1381 ttccgtagtc gtagaagccc cggccgacct tccggccgag acgcccggcc gtgaccatcc
     1441 gccgcagcag cggcggcggc gcgaacgtcg gctccttgaa ctcctcgaag agcacgttcg
     1501 cgatgaatag atgcgtgtcg agcccgatca ggtcgatcag ccggagcggg cccatcgggt
     1561 ggttcaggcc gaggtggacc gccgtgtcga tgtcatcccg cgtcgcgaac ccgtcctcga
     1621 gcatcctgat cgcgccgttc aggtacggga tgagcagcgc gttcacgatg aagccggcgc
     1681 ggtcgcgcga ctcgacggtg gtcttgccga gcacgacgcc gtaggcacgt ccgaactcca
     1741 tcgtgtcgtc cgagctcgag atggccctca cgagctcgat gagccccatc acggggaccg
     1801 ggttgaagaa gtgcatcccg agcaccttgt cgggacgcga cgtcgcggcg ccgaggtccg
     1861 cgatcgggat cgacgacgtg ttgctcgcca ggatcacctc cggcttggcg acctcgtcga
     1921 ggcgacggaa catcacgacc ttcgtgtcgt gatcctcggt cgcggcctcg atcacgaggt
     1981 cgacgtcggc caagtcctgc acgtccgtgg tcagggagat gcgtccgagc accgctgcgc
     2041 gctcctcggc gtcgaggctc ccgcgttcga ccgcgcggag cgtcgaggtc tcgatccgtc
     2101 cacggccccg ctcgaccagc tcgtcgctcg tctcgagcac gacagcggtc tgtcccgcgc
     2161 gagccacgac ctcggagatc cccgagccca tgaggccgca cccgatgacc ccgaccgtcc
     2221 gaacgtcctc cgcgcgcatc gcaccccgat ggtcttgtca ggggccaagc gtacaatcgg
     2281 gcacgctcca tgaaggggaa gggacgcggc gtgcgtgagg aaccgaggct ccgtggactc
     2341 ccgacgttcg ggctcgccat ggccggtctg gtcctcggtc acgtggtcgc ctacctgatc
     2401 gccgtcccgg atccacacca tcgtgcgttc gtcctgcagg agaccggcca cgagtacctg
     2461 cccgcgatgg cggaggcggc actcgtcctg gcgctcgcag ggatggcgac cgtggtgatc
     2521 cgggcgttcg cacccggggc acggcgtggt tccgagcgct tctcgcggct cgccggccgg
     2581 ctcgggctcg tgcaggtgct ggcgttcggg gggctcgagg tcgtggagcg ggtcgtcgtg
     2641 ggagcgccgc ttcgccatct ggcgagcgac cacatcctgg tgatcggcac ggcggtgcag
     2701 gtcgtggtcg ccctcgccgg cgcggcgttc ctccgatggc tcgcgcgaac ctccagcaag
     2761 ctctccgaga cggccctgcc ccgcctcccg ctccctcggg ccggggccgt ccttgcgttg
     2821 gcgagtacat cgtggggtcc cggcatcagc gcgttcggct cggtcggcaa cgtccgggcc
     2881 ccgccctcct cctgaatctc cccctccccg ttcgatcgcg tggccttccg gtcacgcccc
     2941 gacgcgtttg gaggttcgtc catgccgaaa cgatcgatgc gactcccgtc cgccaccgcg
     3001 ctcgtcgtca cgctcctggt gctcatggcc gcgcccgcgt tcgcccatga ggaacgttcc
     3061 gtcggtgact tcgacatggc ggtcggcttc ggggcagagc ctgcgtacgc cggcgaagtc
     3121 aacagcgtgc agctcctcct gtcgaggaat gggaagcccg tcgtcgacct cggcgacacc
     3181 ttggacgtcg aggtcacgtt cggggaccag acgcagaagt tcccgctcga gccgttcttc
     3241 gaggagggcg agttcgggac gctcggggac taccgagcgt ggttcatccc gacccgttcg
     3301 ggtcggtaca cgttccactt ctccggcacg atcgacggcc aggctgtgga cgagtccttc
     3361 acctccgggc cgaacacgtt ctccgacgtg gagtccccac agtcggtcga gttccccgtc
     3421 caggatccga ccacaggtga gctcgccgaa cggatcgatc gcgtggaacc gcgcctcgcg
     3481 agctcgatcg acgacgttca gaccgaggtg caggccaccg ccgacgacgc gtcgagcgcg
     3541 aagacgatcg gcctgatcgg gctcgtggtt ggcgcgatcg gcctgatcgt cgcgatcgtg
     3601 gcgctcgtgg cctcccggag gaagagggtt tgatcggcgt gctggcgcac gccggtggcg
     3661 ttcccgagtc cgtctccacg ggtctgctcg ggctcggcct cgtgaccggc tggatcggcc
     3721 tcagccggct gcgggatcgc gggttcccga gcctgcctcg ctggggtggc tgggcactgg
     3781 tcggcctggc gccggttgcg gtcgtggcct cgatcgccgt gccgtccctc ctgtggcccc
     3841 cgccggcggc gatccgtccc cggtccacgg ccaccctctc gttcgtggag ccgaccccga
     3901 accaggtcgt caccggctcg acgctggacg tgcggttgcg gctcgacggc ggtcgcatcg
     3961 tccagagctc gtcgaccacg ctgacaccgg acaccggcca catccacctg ttcgtggacg
     4021 gggcgatcgt ctcgatgacg tacggcacga agcagcgcgt ccccctgggc gggctcgacc
     4081 ccggacccca cgtcctgcag gccgagttcg tggcggccga tcacggcccg ttcgacccga
     4141 gggtca
//
LOCUS       DATBRK010000231         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_900164, whole genome shotgun sequence.
ACCESSION   DATBRK010000231 DATBRK010000000
VERSION     DATBRK010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900164"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VIH70_07060"
     CDS             <1..295
                     /locus_tag="VIH70_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116156.1"
                     /translation="TFTTPPGATLIAETDIGPQAFVSGRSLGLQFHPEVTPEIMHDWV
                     RAYRHELDGEGVDPDALLAETDRNAEENRRTAMRLLERFLNGVARLGSAATRR"
     gene            292..1635
                     /locus_tag="VIH70_07065"
     CDS             292..1635
                     /locus_tag="VIH70_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8116157.1"
                     /translation="MIRAEGSAVFHRFLDLPYPSIERGEGVWLTTIDGRRILDACSGG
                     AMVACLGHGVRELAAAAAEQAEKISYFYNHHFTSEPQERLADRLLGLAPEMARARFVS
                     GGSEANETALQLARLYHVERGSTDRWRVISPAQAYHGSSMGTLALNGRPAVQEPYAPY
                     LASHLHLPPSTWRLDPTGEAALAELDRLLERHENEIAAFFCEPVSAAALPAYSPPEHF
                     WHGLAERRKEHGFLVCFDEVVTGIGRVGSWLAAHQLPIEPDIVAVGKGLGAGYAQLGA
                     ALVRQEVYDAIDRGSREFDLGHTWDGAPLPSAVGLAVVDLIEERNLVDRVRDRGPGLR
                     DELAEALAGSEIVREVRGRGFLMGVELVDPRDGTSFLPVELDAAEIVDDTAFEHGLLV
                     TSTHPQADRFAGDQVLLAPAYTSTDAELAEMVERFAATMTDVERRVGAALAGAPS"
     gene            1632..>2048
                     /locus_tag="VIH70_07070"
     CDS             1632..>2048
                     /locus_tag="VIH70_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116158.1"
                     /translation="MSARANGAGEEFVLLAIPDMNGSMRGKALRPDAFESALEHGTVM
                     TDLIIGLDPVDAPIADYERFGIRVGAPDLIVRPDPDTIRDLRWRPGWRVCLATPSWPD
                     GSPAFASREVLRRVLEDVDGLGFEVMAAIEYEIRIWD"
BASE COUNT          290 a          740 c          730 g          288 t
ORIGIN      
        1 cacgttcacg acgccgccgg gcgcgaccct gatcgcggaa acggacatcg gtccgcaggc
       61 gttcgtgtcg gggcggagcc tggggctcca gttccacccc gaggtcacgc ccgagatcat
      121 gcacgactgg gtccgcgcgt atcgccacga gctggacggc gagggcgtcg acccggacgc
      181 gttgctcgcc gagaccgacc gcaacgccga ggagaaccgg cgcaccgcga tgcgactgct
      241 cgaacgcttc ctgaacggcg tcgcgcgact cggttcggcc gcgacccggc gatgatccgc
      301 gccgagggct cggccgtgtt ccatcgcttc ctcgacctgc cgtacccgag catcgagcgg
      361 ggcgagggcg tgtggctgac gacgatcgac ggccgccgga tcctcgacgc gtgctccggt
      421 ggcgcgatgg tggcctgcct tggtcacggg gtccgcgagc tcgcggccgc cgccgcggag
      481 caggcggaga agatctcgta cttctacaac caccacttca cgagcgagcc acaggaacgg
      541 ctggcggatc gactgctcgg actggcgccg gagatggcac gggcccggtt cgtgtccggg
      601 ggttcggagg cgaacgagac ggccctgcag ctcgcccggc tctaccacgt cgaacgcgga
      661 tcgaccgacc gctggcgcgt gatctcgccc gcgcaggcgt accacgggtc ctccatgggc
      721 acgctggccc tcaacggccg gccggctgtc caggagccgt acgcgccgta cctcgcgtcg
      781 cacctgcacc tgcccccgtc gacgtggcgc ctcgacccga ccggcgaggc cgccctcgcc
      841 gagctcgacc gcctgctcga gcggcacgag aacgagatcg cggcgttctt ctgcgaaccc
      901 gtgagcgcgg ccgcgctccc cgcctactcg ccacccgaac acttctggca cggcctcgcc
      961 gaacgccgga aggagcacgg gttcctggtc tgtttcgacg aggtggtgac cggcatcggc
     1021 cgcgtcggca gctggctcgc cgcgcaccag ctgcccatcg agcccgacat cgtcgccgtc
     1081 ggcaaggggc tcggggcggg gtacgcgcag ctcggagccg ccctggtccg gcaggaggtc
     1141 tacgacgcga tcgaccgagg gtcgcgcgag ttcgacctcg ggcacacgtg ggacggggcg
     1201 ccgctgccct ccgccgtcgg gctcgccgtc gtcgacctca tcgaggaacg gaacctcgtg
     1261 gatcgggttc gcgaccgggg tcccggcctg cgggacgagc tcgccgaagc gctcgccggc
     1321 tcggagatcg tcagggaggt ccgcgggcga ggcttcctca tgggcgtgga gctcgtcgac
     1381 ccgcgcgacg gcacgtcgtt cctgcccgtg gagctcgacg cggccgagat cgtcgacgac
     1441 accgcgttcg aacacggcct gctggtgacg tccacccatc cgcaggcgga taggttcgcc
     1501 ggcgaccagg tgctcctcgc gcccgcctac accagcaccg atgcagagct cgccgagatg
     1561 gtcgaacgct tcgcggcgac gatgaccgac gtcgagcgac gcgtcggcgc cgccctggcg
     1621 ggcgcgccat cgtgagcgcc cgagcgaacg gcgccggcga ggagttcgtc ctcctcgcga
     1681 tcccggacat gaacgggtcg atgcgtggca aggccctgcg cccggacgcg ttcgagtcgg
     1741 ccctggaaca cggcaccgtg atgaccgacc tcatcatcgg gctcgatccg gtcgacgcgc
     1801 cgatcgccga ctacgagcgc ttcgggatcc gcgtgggcgc gcccgacctg atcgtccgcc
     1861 cagaccccga cacgatccgc gatctccgat ggcggccggg gtggcgcgtg tgcctggcga
     1921 cgccgagctg gcccgacggg tcgcccgcgt tcgcctcgcg cgaggtcttg cgtcgcgtgc
     1981 tggaggacgt cgacgggctc ggtttcgagg tcatggccgc gatcgagtac gagatcagga
     2041 tctgggac
//
LOCUS       DATBRK010000232         8932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2638014, whole genome shotgun sequence.
ACCESSION   DATBRK010000232 DATBRK010000000
VERSION     DATBRK010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8932
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2638014"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..87)
                     /locus_tag="VIH70_07075"
     tRNA            complement(2..87)
                     /locus_tag="VIH70_07075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Leu,seq:gag)
     gene            complement(129..356)
                     /gene="secG"
                     /locus_tag="VIH70_07080"
     CDS             complement(129..356)
                     /gene="secG"
                     /locus_tag="VIH70_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY8116159.1"
                     /translation="MFNIIALVLCVISSLFLIVFILLHSGKGSGLSDMFGGTSSLSGG
                     TALERRLDKITVVTAVIFGTSTFWLSWKWGG"
     gene            complement(379..1440)
                     /locus_tag="VIH70_07085"
     CDS             complement(379..1440)
                     /locus_tag="VIH70_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8116160.1"
                     /translation="MSVEVTERSQPEPTTERTPLVRIRGLRKLFPITRGILFQKRIGD
                     VHAVDGVDLEIFKGETMGLVGETGCGKSTLARVVMRLYDASEGTIEFEGQDITHAKGG
                     DLRELRREMQMIFQDPFASLNPRKTIGSIIGAPYRLHKVVPRNKIKSEVQQLMELVGL
                     NPEHYNRYPHEFSGGQRQRIGVARSLALRPKLIVCDEPVSALDVSIQAQVLNLLEDLQ
                     EEFNLTYLFIAHDLSVVKHVSDRVAVMYLGKIVEMAVGQTLYRNPKHPYSGALLSAVP
                     IPDPDLAAAKKRIILEGDVPSPIDPPSGCRFHPRCPNAQFPKCSEEEPELLPHHPGQV
                     AACHFPLEDRALIETPADR"
     gene            complement(1437..2453)
                     /locus_tag="VIH70_07090"
     CDS             complement(1437..2453)
                     /locus_tag="VIH70_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8116161.1"
                     /translation="MAEPLLEVKDLSVHFPTDDGLVKAVDGVSFSLQPGETLGVVGES
                     GSGKSVSFLTVMGLISRKQAVIEGEIIFQGQDLLTLPPDEMRTVRGEKISMIFQDPMT
                     SLHPYYKVGDQIAEAVRVHQQVSKQEAHEQAVEMLRRVGIPRPDERADQYPHEFSGGM
                     RQRAMIAMALSLNPDLLIADEPTTALDVTVQAQILDLLDRLKQEFNAAIVIVTHDLGV
                     VAEHCDHIQVMYAGKIVEAGDRRDIYYGAHHPYTWGLLSSIPRLNESDDKERLVPIRG
                     LPPSLIFVPPGCAFHPRCPHVFDRCRTEVPELLPADGHHASRCHLSLADKERIFQEEV
                     MARS"
     gene            complement(2453..3424)
                     /locus_tag="VIH70_07095"
     CDS             complement(2453..3424)
                     /locus_tag="VIH70_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8116162.1"
                     /translation="MGRYLIRRVLFMILVLFVVSLITFIIFVKLPASDPARRAAGKQT
                     TPENIAAARHAFGLDRPLYVQYGRFAKGLIPWPGLFLNEDVYYSYSNFVPVKEEIFSR
                     LPVTITLAVGAAITWVLIGIPIGIISAVRRGSVWDRASMVFALIGVSAPVFWLAYLFL
                     YIFWFKLGWAPSSGIPIGESVTEAIFSGVFILPWIVLSLTFAAFYARMVRGNLIETMG
                     EDYIRTARAKGLSEKRVIYKHGLRAALTPVVTMFGLDLATLLGGAFITETVFNLPGIG
                     QYAVRSIFTNDFPSVMGVTLFGAFFIALANLCVDIAYAFLDPRVRYT"
     gene            complement(3547..5424)
                     /locus_tag="VIH70_07100"
     CDS             complement(3547..5424)
                     /locus_tag="VIH70_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8116163.1"
                     /translation="MRRYLSVLALVAVLGLVAAACGGDNEGATGATGATATGPSGELQ
                     PGGTVELAMLGDVSAAFDPQKEYYSVTWEWFRCCLLRTLLSYNGRTTAEGGADLQPDI
                     ASALPEISSDGLTYTFTLKSGVAYGDPFGDVTVTSGDIVRALERESDPDASVGGYNFY
                     YNVIDGFEDFGAGKADSIKGLETPDDSTLVVTLTEPAGYLPYLFSMDASAPIPPNGDA
                     PLGAAEGHTRDYGRFLVATGPYQFLGSENMDFSVPADEQTPVAGYTPGKSFQLVRNPN
                     YDPATDGLRPAYPDGFDVSIGGDNNDLYNKVQAGELDGVVDGIVPPPVLRSYSTDPTL
                     QPLLHINPSDAVRYVSFNVAEPPFDDVHIRKAFNLALDKAGMRTVRGGESVGEIAGHI
                     MVNSLENNLLADYDPYATPNSAGDVTAAQAEIAQSKYDTDGDGVCDAPECSNILSATD
                     EADPYPDQAALIQQNMEPLGLTFDVKSFERTTMYAKCNEPEAHVAVCLAPAWGKDFPD
                     GYTFADPLFGSTAIFPSCCNYQLTGASPELLSKAGYDVTDVPSVDDQIKAANAKTGDA
                     RYQAWADLDTTLMEQVVPWAPYLFDNNVDVTSARIINYSFDQFAGLAAYDQYAISPDA
                     Q"
     gene            complement(5550..6548)
                     /locus_tag="VIH70_07105"
     CDS             complement(5550..6548)
                     /locus_tag="VIH70_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018531257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8116164.1"
                     /translation="MARAHVEHALTDSGPDAPIDPEATSVEVPQQVIGRSPWELFWRR
                     FRRDKFALGGAFIVILMLILALLAPVIAGFTHGPNDVHLDRLDSFGIPTGPQSGFPFG
                     VDTAGRDLFVRVIYGARTSLIIAFFATGMASLIGVTLGMLAGFYRGKVDTLISRTIDV
                     VLSLPVLLLALGLVSACGLSPHGCLGGIIKPGLLLVSYVIALFTWPYLARIVRGQVLS
                     LREKEFIESARSLGSTNRRIMFREVLPNVAAPIIVYTTLIIPNNILFEAALSFLGIGV
                     PQETPSWGRELSDAGTIFTVAWWMMLFPGLFLFFTTLAFNLVGDGLRDALDPRTGG"
     gene            complement(6703..7965)
                     /gene="apgM"
                     /locus_tag="VIH70_07110"
     CDS             complement(6703..7965)
                     /gene="apgM"
                     /locus_tag="VIH70_07110"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY8116165.1"
                     /translation="MNRLLYVCLDGLADDPIPEFDGRTPLEAADTPFLDALARRGRTG
                     TVTTVGEGIAPESDIAVFAILGYDPREEHPGRGVVEALGVGMDFRDGDLAYRVNFATA
                     DWPTIVDRRVGRDLTSDEAGALADEVNAKLSLPNATFDLRSTVEHRGVLVIRANGETL
                     SAEVSNTDPAYRKDGHLGVALETFEPVVARCEPLDDTAEAGRAAALTNAFVEGAAKIL
                     DASEVNAARRRAGRLPANLILTRDGGDRRPSLQPIAERFGPSWGCFVEMPVERGIALI
                     LGMDPVDVPRLRAEEDYATWASLTIDALDGFDALYVHIKGPDVPAHDGRAEQKRDVIG
                     LIDRAFFGELLPGIDPARTIVAVTADHATSCLRKAHTAEPVPLLVSGGPVRPDGTEAF
                     GERACASGKLGALKGIEILPRISPLLRE"
     gene            complement(7970..8740)
                     /gene="tpiA"
                     /locus_tag="VIH70_07115"
     CDS             complement(7970..8740)
                     /gene="tpiA"
                     /locus_tag="VIH70_07115"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY8116166.1"
                     /translation="MSDRRPIIAANWKMHKTHLEAIQAVQKLSYLLGQDDAERVEVVI
                     CPAATSLRSVQTLIDSDRLPYGLGAQNVHWQDQGAFTGEISPPMLVALRVGYVIVGHS
                     ERRATFGETDETVNKKVRAIFAHGMTPILCVGETLRERDVGATQDRVTAQVRAALAGV
                     SSSDAAKLVVAYEPVWAIGTGRNAEPSDAGQVIALIRQTIADLHEADLARAVRIQYGG
                     SVKAGNIRDFMAHPEVDGALVGGASLDPEELALIVKYR"
     gene            complement(8783..>8932)
                     /gene="pgk"
                     /locus_tag="VIH70_07120"
     CDS             complement(8783..>8932)
                     /gene="pgk"
                     /locus_tag="VIH70_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016880330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY8116167.1"
                     /translation="VVGGGDSLAAVKKYGLEGGFDHLSTGGGASLEFIEGRELPGIAV
                     LEVAT"
BASE COUNT         1457 a         3042 c         2987 g         1446 t
ORIGIN      
        1 gtgtcggagg ggggatttga acccccacgg cccatagggc cactaggccc tcaacctagc
       61 gcgtctacca agttccgcca ctccgacggg agtctggcga ttctagcaac cgggcacgag
      121 cgctccggtc agccgcccca cttccacgac agccagaagg tcgacgtgcc gaagatcacg
      181 gcggtgacca cggtgatctt gtcgaggcgt cgctcgagcg cggtgcctcc cgacaggctc
      241 gacgtaccgc cgaacatgtc cgacaggccg ctccccttgc cggagtggag caggatgaac
      301 acgatcagga acaggctcga gatcacacac agcacgagcg cgatgatgtt gaacatggcc
      361 cgtcagtgta ccggctctct acctgtccgc cggggtctcg atgagcgctc gatcctccag
      421 cgggaagtgg caggccgcga cctggccggg atgatgcgga aggagttccg gctcctcctc
      481 gctgcacttg gggaactgcg cgttcggaca gcgggggtgg aaccggcagc ccgagggcgg
      541 gtcgatcggg ctcgggacgt cgccctcgag gatgatgcgc ttcttggcgg cggccaggtc
      601 cgggtccggg atcgggaccg ccgagagcag ggctcccgaa tacgggtgct tggggttgcg
      661 gtagagcgtc tgccccacgg ccatctcgac gatcttcccc aggtacataa ccgcgacccg
      721 gtcggagacg tgcttcacga ccgagaggtc gtgcgcgatg aacaggtacg tgaggttgaa
      781 ctcctcctgg aggtcctcga gcaggttcag cacctgggcc tggatcgaga cgtcgagcgc
      841 cgacaccggc tcgtcgcaca cgatcagctt gggccgaagc gcgagcgagc gagcgacccc
      901 gatccgctga cgctggccgc ccgagaactc gtgcggatag cggttgtagt gctcggggtt
      961 caggcccacc agctccatca gctgctgcac ctccgacttg atcttgttgc gcggcacgac
     1021 cttgtgaagc cggtaggggg ctccgatgat cgacccgatg gtcttgcgcg ggttcagcga
     1081 cgcgaacggg tcctggaaga tcatctgcat ctcgcgacgg agctcgcgca gatcgccacc
     1141 cttcgcatgc gtgatgtcct ggccctcgaa ctcgatcgtc ccctccgacg cgtcgtacag
     1201 gcgcatcacc acacgggcga gcgtcgattt cccgcacccc gtctccccca ccagccccat
     1261 cgtctcgccc ttgaagatct ccagatccac gccgtccacc gcgtgcacat cgccgatccg
     1321 cttctggaac aggatgccgc gggtgatcgg gaagagcttg cgcaacccac ggatgcggac
     1381 gagcggcgtc cgctcggtcg tcggttcggg ctgcgatcgt tcggtgacct cgacgctcac
     1441 gagcgggcca tgacctcctc ctggaagatg cgttccttgt cggcgagcga caggtggcac
     1501 cgcgacgcgt gatggccgtc cgccggcaag agctctggca cctcggtccg gcaccggtcg
     1561 aacacatgcg ggcaacgggg atggaacgcg cagccgggtg ggacgaagat cagggacggc
     1621 gggaggccgc ggatcgggac cagtcgctcc ttgtcgtcgc tctcgttgag ccgggggatc
     1681 gacgacagca ggccccacgt gtacggatgg tgggctccgt aatagatgtc gcggcggtcg
     1741 ccggcctcga cgatcttgcc ggcgtacatc acctggatgt ggtcgcagtg ctcggccacc
     1801 acgccgagat cgtgcgtgac gatcacgatc gccgcgttga actcctgctt cagccggtcg
     1861 agcaggtcga ggatctgcgc ctggaccgtc acgtcgagcg ccgtcgtggg ctcgtcggcg
     1921 atgagcaggt ccgggttcag cgagagcgcc atcgcgatca tcgcccgctg acgcatgccg
     1981 ccggagaact cgtgcgggta ctggtcggcg cgttcgtccg gacgggggat ccccacgcgc
     2041 cggagcatct cgacggcctg ctcgtgggcc tcctgcttcg agacctgctg gtggacgcgg
     2101 accgcttcgg cgatctggtc gccgaccttg tagtagggat gaagcgaggt catcgggtcc
     2161 tggaagatca tcgagatctt ctcgccgcgg acggtgcgca tctcgtcggg ggggagcgtc
     2221 agcaggtcct gcccctggaa gatgatctcg ccctcgatca cggcctgctt cctgctgatg
     2281 agccccatca ccgtgaggaa cgagacgctc ttgccggaac ccgactcccc cacgaccccg
     2341 agcgtctcgc ccggctgcaa cgagaacgag acgccgtcga ccgccttcac caatccgtcg
     2401 tcggtcggga agtggacgct gaggtccttg acctcgagga gcggttccgc cactacgtgt
     2461 acctcacccg cgggtcgagg aacgcgtagg cgatgtcgac gcacaggttg gcgagcgcga
     2521 tgaagaacgc gccgaagagg gtgacaccca tcacggacgg gaagtcgttc gtgaagatgc
     2581 tccgcaccgc gtactggccg atgcccggca ggttgaagac ggtctcggtg atgaacgcgc
     2641 ccccgagcag cgtcgccagg tccagaccga acatcgtcac caccggagtc agggctgcgc
     2701 gcagcccgtg cttgtagatg acccgcttct cggaaagtcc cttggcgcgg gcggtgcgga
     2761 tgtagtcttc gcccatcgtc tcgatcaggt tcccccgcac catccgcgcg tagaacgccg
     2821 cgaacgtcag cgacagcacg atccacggga ggatgaacac gcccgagaag atcgcctcgg
     2881 tcaccgattc cccgatcggg atccccgacg atggggccca gcctaacttg aaccagaaga
     2941 tgtacaggaa caggtaggcg agccagaaca ccggcgccga cacgccgatg agggcgaaca
     3001 ccatggacgc tcggtcccag acgcttccac gtctgaccgc cgagatgatc ccgatcggga
     3061 tcccgatcag gacccaggtg atggcggccc ctaccgcgag cgtgatcgtc accggcaggc
     3121 ggctgaagat ctcctccttg accgggacga aattgctgta ggagtagtag acgtcctcgt
     3181 tgaggaacag ccccggccac gggatcaaac ccttggcgaa ccgtccgtat tggacgtaga
     3241 ggggccggtc gagcccgaac gcgtgccggg ccgccgcgat gttctccggg gtggtctgct
     3301 tgcccgcggc tcgccgtgcc ggatcggatg cgggcagctt gacgaagatg atgaaggtga
     3361 tcagggatac gacgaacagg accaggatca tgaacagcac ccgtcggatc agatacctgc
     3421 ccatcgcgcc ttccggagtc tactccactc gggaggaggg gccgccccct cggagcggcc
     3481 cctccccttc ctcatcctgc ctcgttcttg cggtttcctg ccctcctcgt gccccctcgg
     3541 cccctgctat tgagcgtcgg gcgagatggc gtactggtcg tacgccgcca ggccggcgaa
     3601 ctggtcgaac gagtagttga tgatccgcgc cgatgtgacg tcgacgttgt tgtcgaacag
     3661 gtacggcgcc cacgggacca cctgttccat cagcgtggta tcgaggtcgg cccaagcctg
     3721 gtaccgggcg tcccccgtct tcgcgttcgc ggccttgatc tgatcgtcga ccgacggaac
     3781 gtcggtgacg tcgtagccgg ccttcgacag gagctcggga ctcgcgccgg tgagctggta
     3841 gttgcagcag ctcgggaaga tcgcggtcga gccgaacagc ggatccgcaa acgtgtaccc
     3901 gtccgggaag tccttgcccc aggcaggggc gagacagacg gcgacgtggg cctccggctc
     3961 gttgcacttg gcgtacatcg tcgtgcgctc gaaggacttc acgtcgaacg tcagaccgag
     4021 cggctccatg ttctgttgga tcaacgcggc ctggtccgga tacgggtccg cctcgtccgt
     4081 cgcggagagg atgttggagc actccggcgc gtcacacacg ccgtcgccgt ccgtgtcgta
     4141 cttggactgc gcgatctccg cctgcgccgc cgtgacgtcc cccgccgagt tcggcgtggc
     4201 gtacgggtcg tagtcagcga ggaggttgtt ctccagcgag ttcaccatga tgtggccggc
     4261 gatctcgccg acggactcac cgccccggac cgtccgcatc cccgccttgt ccagggcgag
     4321 gttgaaggct tttcggatgt ggacgtcgtc gaacggtggc tcggcgacgt tgaacgacac
     4381 gtaccgcacg gcgtccgacg ggttgatgtg cagcaggggc tgcagcgtcg ggtccgtcga
     4441 gtacgagcgc aggacgggtg gcggcacgat gccgtcgacc acgccgtcca actcgccggc
     4501 ctgcaccttg ttgtacaggt cgttgttgtc gccgccgatc gagacgtcga acccgtccgg
     4561 ataggcggga cggagaccgt cggtcgctgg gtcgtagttc gggttccgca cgagctggaa
     4621 cgacttgccg ggcgtgtacc cggccaccgg tgtctgctcg tcggccggca ccgagaagtc
     4681 catgttctcc gaaccgagga actggtaggg gcccgtggcc accaggaagc ggccgtagtc
     4741 cctcgtgtgt ccctccgccg cgccgagcgg agcgtcgccg ttcggcggga tcggcgccga
     4801 ggcgtccatc gagaacagat acggcaggta gccggccggc tccgtcagcg tcaccaccag
     4861 cgtgctgtcg tcgggggtct cgaggccctt gatcgagtcg gccttgcccg cgccgaagtc
     4921 ctcgaacccg tcgatgacgt tgtaatagaa gttgtagccg cccaccgacg cgtctgggtc
     4981 ggactcgcgc tcgagcgcgc ggacgatgtc cccggacgtg acggtgacgt cgccgaacgg
     5041 atccccgtac gccacgccgc tcttgagcgt gaacgtgtag gtcaggccgt ccgacgagat
     5101 ctccggcagg gcgctcgcga tgtcgggctg caggtcggct ccgccctccg ccgtggtcct
     5161 cccgttgtac gacaggagcg tccggagcag acaacaccgg aaccactccc acgtgaccga
     5221 gtagtactcc ttctgcgggt cgaacgcggc actcacgtcg ccgagcatcg cgagctcgac
     5281 ggtgccgccg ggctgaagct cgccgctcgg gccggtcgcc gtggctccgg tcgcaccggt
     5341 cgcaccctcg ttatcgccgc cacatgccgc cgcgacgaga cccagtacgg cgacgagcgc
     5401 cagtacgctc agatatcgcc tcacgtaacc cccctctcct cgtgcttggt tcctaccgtc
     5461 gcccgggccg ggcccgggtc catctactcc tgttcaggca cgccgttcct tccctccttt
     5521 cgggaaagct gcctcccgtc tagatacggt cagccgcccg tcctgggatc gagggcatct
     5581 cggagcccgt ccccgaccag gttgaaggcg agcgtcgtga agaacaggaa cagtccgggg
     5641 aacagcatca tccaccaggc cacggtgaag atcgtcccgg catcggacag ctcccgcccc
     5701 caggaggggg tctcctgagg gacgccgatg ccgaggaacg acagggcggc ctcgaacagg
     5761 atgttgttgg ggatgatcag cgtcgtgtag acgatgatcg gcgccgcgac gttggggagc
     5821 acctcgcgga acatgatccg gcggttggtc gagcccaggg agcgcgccga ctcgatgaac
     5881 tccttctcgc gcagcgacag cacctggcca cgcacgatgc gggcaaggta cggccaggtg
     5941 aacagcgcga tcacgtagct gaccagcagc aggcccggct tgatgatccc gcccaggcag
     6001 ccgtggggcg acagcccgca cgccgaaacg agcccgaggg ccagcagcag gaccggcagg
     6061 gacaggacga cgtcgatcgt tcgggagatc agggtgtcga ccttccctcg gtagaagccc
     6121 gcgagcatcc cgagcgtcac accgatcagg gacgccatcc ccgtcgcgaa gaacgcgatg
     6181 atcagggacg tccgagcccc gtagatcaca cggacgaaca ggtccctccc ggccgtgtcg
     6241 accccgaagg ggaacccgct ctgggggccg gtcgggatcc cgaacgaatc gagccgatcc
     6301 aggtgcacat cgttcgggcc gtgcgtgaaa cccgcgatca cgggagcgag gagagcgagt
     6361 atgagcatga ggatgacgat gaacgccccg ccgagcgcga acttgtccct gcggaaccgg
     6421 cgccagaaca actcccacgg agagcggccg atgacctgct gcggaacctc gacgctggtc
     6481 gcttccggat cgatgggcgc gtcgggaccc gagtcggtga gcgcgtgctc cacgtgggct
     6541 cgtgccatct ggtgcgcctg taccccttcc cggatcggga cggcgcatct tagccctcgg
     6601 tcccgtggcc aagcaatgcg ccggaaatcc aactatacgc cgctcggaaa tctcggggtt
     6661 gcggcatctt gcggtcggcc tgccgaacgt cgaggcgcgg cgtcactccc gcagcagcgg
     6721 ggagatccgg gggaggatct cgatgccttt caacgcacct agcttccccg aggcgcacgc
     6781 acgttcgccg aacgcctcgg ttccgtccgg acgaaccggc ccgcccgata ccaacagggg
     6841 gaccggctcc gcggtgtggg ccttgcggag gcacgaggtt gcgtggtccg ccgtcacggc
     6901 cacgatcgtc ctcgcggggt cgatccccgg gagaagctct ccgaagaacg cccggtcgat
     6961 caggccgatc acgtcgcgct tttgctcggc gcggccgtcg tgggccggga cgtccggacc
     7021 cttgatatgc acgtacagcg cgtcgaagcc gtcgagcgcg tcgatcgtga ggctggccca
     7081 ggtcgcgtag tcctcctcgg cacgcagccg aggaacgtcc acggggtcca tgccaaggat
     7141 caacgcgatc ccgcgttcga cgggcatctc gacgaagcac ccccacgatg gcccgaaccg
     7201 ctccgcgatc ggctgcaggg acggccggcg gtctccgccg tcgcgcgtca ggatcaggtt
     7261 cgcaggcagc ctgcccgccc gccgtcgcgc ggcgttgacc tccgatgcat cgaggatctt
     7321 cgcggcgccc tcgacgaacg cgttggtgag cgcggccgcc cgcccggcct ccgccgtgtc
     7381 gtcgagcggc tcgcaccggg ccaccaccgg ctcgaacgtc tcgagcgcca cgccgaggtg
     7441 tccgtccttg cggtacgccg ggtcggtgtt ggagacctcg gcggagagtg tctccccgtt
     7501 cgcgcggatc accaggacgc cccgatgctc caccgtggag cgcaggtcga acgtggcgtt
     7561 cgggagcgag agcttcgcgt tcacctcgtc cgcgagggcc cccgcctcgt cggaggtcag
     7621 atcgcggccg acgcgccggt cgacgatcgt gggccaatcc gcggtggcga agttcacgcg
     7681 gtacgcgagg tcgccgtcgc ggaagtccat gcccaccccg agggcctcga cgacgcctcg
     7741 tcccggatgc tcctcgcgcg ggtcgtaccc caagatcgcg aacaccgcga tgtccgactc
     7801 gggcgcgatc ccctcgccga cggtcgtcac ggtccccgtt ctgccccgcc gcgccagcgc
     7861 atcgaggaac ggcgtgtcgg ctgcctccag cggggtacgg ccgtcgaact cggggatggg
     7921 gtcgtcggcc aggccgtcca ggcagacgta gagcagccgg ttcacaagat caccggtact
     7981 tgacgatcag ggcgagctcc tccggatcca ggctggcccc gccgacgagc gccccgtcga
     8041 cctcggggtg tgccatgaag tcgcggatgt tccccgcctt cacgctcccg ccgtactgga
     8101 tccggaccgc ccgggccagg tccgcttcat gcagatcggc gatcgtctgc ctgatcaagg
     8161 cgatcacctg gcccgcgtcg gacggctcgg cgttgcgacc ggtgccgatc gcccagacgg
     8221 gttcgtaggc gacgaccagc ttcgcggcgt cggacgacga cacgcccgcc agggccgcgc
     8281 ggacctgggc cgtcacgcgg tcctgggtgg ctccgacgtc gcgctcccgc agcgtctcgc
     8341 cgacacacag gatcggcgtc atcccgtggg cgaagatcgc gcgcaccttc ttgttcacgg
     8401 tctcgtcggt ctcgccgaac gtcgcacggc gctccgagtg cccgacgatc acgtagccca
     8461 ccctgagcgc caccagcatc gggggcgaga tctcgccggt gaacgccccc tggtcctgcc
     8521 agtgcacgtt ctgcgcgccc agaccgtacg ggagccggtc gctgtcgatc agcgtctgca
     8581 ccgagcgcag cgaggtcgcg gccggacaga tcaccacctc cacgcgttcg gcatcatcct
     8641 ggcccagcag gtaggagagc ttctgcacgg cctggatggc ctcgaggtgc gtcttgtgca
     8701 tcttccagtt ggccgcgatg atcggccggc ggtcgctcac tgggccgtcc cccggaggct
     8761 catcgggccg tctcccggac gctcacgtgg cgacctccag cacggcgatg ccgggcagct
     8821 cgcgcccctc gatgaactcg agcgaggcgc ctccgccggt cgagaggtgg tcgaacccgc
     8881 cctccagccc atatttcttc acggccgcga ggctgtcgcc accgccgacg ac
//
LOCUS       DATBRK010000233         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2348081, whole genome shotgun sequence.
ACCESSION   DATBRK010000233 DATBRK010000000
VERSION     DATBRK010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348081"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1068
                     /locus_tag="VIH70_07125"
     CDS             253..1068
                     /locus_tag="VIH70_07125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8116168.1"
                     /translation="MERDPWELAFEAARAAGVELRPLAELEDADRILRVMDATWAGPE
                     PFPREVLRALADSGNVPYGAFDGDETIGYVLGWVGVDPEDGLHTHSHMLAALPGRRHR
                     GVGYALKLAQRAQALDQGIAEVRWTFDPLVARNGYFNLHKLGAVADRFDRNHYGAMVD
                     EVNAGERSDRFTVRWDLGRDPGPRDVRAHEAALEDDGGWPGDVHAPSGRGALVAVPSD
                     YARLRTDDPACAAAWRDASADAIEACLTAGMIAAGFDRVASSYAFAPAEDVPR"
     gene            1065..2177
                     /gene="menC"
                     /locus_tag="VIH70_07130"
     CDS             1065..2177
                     /gene="menC"
                     /locus_tag="VIH70_07130"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEY8116169.1"
                     /translation="MTRIRSIELRLVAIPLVRPFRTSLGESTEKVCILARAETDDAVG
                     WGECVGDVEPTFSEEWNDGAWLMIRDFLAPALFAAGDVEAELVPSVLRFVRGHPMAKA
                     TLVNAVLDAELRAQGRSLASYLGAVRDRVECGVSVGIAPSAEVLLEQVAGYLAEGYRR
                     IKLKIEPGADLERVRAVREANPEILLSVDANAAYRLADVDVFRRLDAFGLLMIEQPLH
                     HEDLWEHSMLQREIRTDICLDESIRSAADARAAIELGACRIVNIKQGRVGGLLEAKRV
                     HDVASELGVPVWCGGMLETGIGRSTNLALAALPNFRLPGDTSATSRYFAEDLTEPLVL
                     APDGTLPVPDGPGIGVEPDPLRLEAATRRTERLKKE"
BASE COUNT          354 a          891 c          972 g          377 t
ORIGIN      
        1 cttcggcagg ctgatgaaga tcgcgttcat cgcggcgatc gtggcggccg tcgtagccgt
       61 cttcaaacgc cggcgcggca aggatctgga cgaggacgag tggcaggagc tgccgccgcc
      121 ctcgggcggt tagccgagcc ggatacgttc cgccgagggg cccgcgctcg tcgcgggccc
      181 ttcgcgttgt ggccgagggc cctgccggtg ccggtcccgg cgtgggtccc gggatggcta
      241 gactccggcg ccgtggagcg cgacccatgg gagctggcgt tcgaggcggc ccgcgccgcc
      301 ggcgtcgagc tccgaccgct cgccgagctc gaggacgcgg atcgcatcct gcgggtcatg
      361 gacgccacgt gggcgggccc cgagcccttc ccgcgcgagg tgctccgcgc gctcgccgac
      421 agcgggaacg tcccgtacgg cgcgttcgac ggcgacgaga cgatcgggta cgtgctggga
      481 tgggtcgggg tggatcccga ggacgggctg catacccact cgcacatgct cgccgccctg
      541 cccggtcggc gtcatcgagg ggtcgggtac gcgctcaagc tcgcgcagcg cgcgcaggcg
      601 ctcgaccagg ggatcgcgga ggtccggtgg accttcgacc ccctcgtcgc ccgcaacggg
      661 tacttcaacc tgcacaagct cggcgcggtc gccgatcgct tcgaccgcaa ccattacggg
      721 gcgatggtcg acgaggtgaa tgccggcgag cggagcgacc ggttcaccgt tcgctgggac
      781 ctcggacgcg acccaggccc gagggacgtg cgcgcgcacg aggcagcgct cgaggacgac
      841 ggcggatggc cgggcgacgt ccatgcgccg tccgggcgcg gggcgctcgt cgcggtcccg
      901 agcgactacg cgcggcttcg gaccgacgat cccgcgtgcg cggcggcgtg gcgcgacgcg
      961 tcggccgatg cgatcgaggc ctgtctcacc gccgggatga tcgcggcggg gttcgaccgc
     1021 gttgcctcga gctacgcgtt cgcgccggcc gaggacgtgc cccggtgacg cggatccgtt
     1081 cgatcgagct tcgcctggtg gcgatcccgc tggtccggcc gttccggacc agcctcggcg
     1141 agtcgaccga gaaggtctgc atcctcgccc gcgcggagac cgacgacgcg gtcggatggg
     1201 gggagtgcgt cggcgacgtc gagcccacct tctccgagga gtggaacgac ggggcctggc
     1261 tgatgatccg cgacttcctc gcacccgcgt tgttcgccgc gggggacgtc gaggccgagc
     1321 tcgtcccctc ggtcctgcgg ttcgtccgcg ggcacccgat ggccaaggcc acgctcgtga
     1381 acgcggtgct cgacgccgag ctccgggcgc agggacgttc gctcgcctcc tacctggggg
     1441 cggtccgaga ccgggtcgag tgcggcgtct cggtcgggat cgccccctcg gccgaggtgt
     1501 tgctcgagca ggtcgcgggc tacctcgccg agggctaccg ccgcatcaag ctcaagatcg
     1561 agccgggcgc cgatctcgaa cgcgtccgcg cggtccggga ggcgaacccc gagatcctgc
     1621 tctcggtgga cgcgaacgcc gcctaccggc tcgcggacgt cgacgtgttc cggcggctcg
     1681 acgcgttcgg gctgttgatg atcgaacagc cgttgcacca cgaggacctg tgggagcact
     1741 cgatgctcca acgggagatc cggaccgaca tctgtctgga cgagtcgatc cgctcggccg
     1801 cggacgcccg ggcggcgatc gagctcggag cgtgccggat cgtcaacatc aagcaggggc
     1861 gcgtcggcgg actgctcgag gccaagcgcg tccacgacgt cgcgtcggag ctcggagtgc
     1921 cggtgtggtg cggggggatg ctcgagaccg ggatcgggcg ttcgacgaac ctcgcgctgg
     1981 ccgcgctccc gaacttccgg ctacccggcg acaccagcgc gacgtcgcgg tacttcgccg
     2041 aggacctgac cgagccgctc gtcctggcgc ccgacgggac gctgccggtc cccgacggac
     2101 ccgggatcgg tgtcgaaccc gatcccctgc gactcgaggc cgccacccgg cggacggagc
     2161 ggttgaagaa agagtgacaa aagcgttcgg gccgggttga ccgtcgcgat gggcgtccgt
     2221 acagtgccgt ttgagagacc gctgtgacct tcgggtcgcg gcggtttctc acatcccggg
     2281 cctcgggtcc ccgcacacct cccgtcgatg acccgagcgc ggcgtcggtg acgccatcgc
     2341 gggccgcttc gaggacgcaa ggcgcacatc gcggagcccg ctcgtcctag actcaggtcg
     2401 acgtccggtc gaggagagct gcgggatgcg caaacgggtc atcagggcgg gcgagatcga
     2461 gatcgaggac tcggaggggc gggtgcgcgc ccggttcggc gtctcgaacg acgatctccc
     2521 gttcatgtcg ttctacggcg cggacgacaa gctgcgcgca cgcttcgggg tgcagcccga
     2581 cggctcggcc ggcc
//
LOCUS       DATBRK010000234         5665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1627468, whole genome shotgun sequence.
ACCESSION   DATBRK010000234 DATBRK010000000
VERSION     DATBRK010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627468"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..528
                     /locus_tag="VIH70_07135"
     CDS             13..528
                     /locus_tag="VIH70_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8116170.1"
                     /translation="MAGVRKPKVDKSGRTSAGILLWRSRGGRLEVLLGHPGGPYFAGK
                     DADHWTVLKGEVDPGEDLLAFARREFEEETGFPPPEGPTIELGEIRQKSGKRVLAWAV
                     GGDLDTQTAVSNSFEMEWPPRSGRMREFPEIDRVEWFGLDDARGKIKAAQAPFLDRLE
                     RTLAEEHDQAR"
     gene            complement(532..903)
                     /locus_tag="VIH70_07140"
     CDS             complement(532..903)
                     /locus_tag="VIH70_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116171.1"
                     /translation="MKVLKIGFVGTRTAQPAAMVDLFERTLALRPMHGGDDMWAFRLP
                     DGGIAEVFGPTQNDHLTTGPVVEFLVEDVAAATEELRAAGVPILLGPVRSDEAGLTWT
                     HFRAPDGNVYGVIETDAARDA"
     gene            complement(957..1355)
                     /locus_tag="VIH70_07145"
     CDS             complement(957..1355)
                     /locus_tag="VIH70_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEY8116172.1"
                     /translation="MKAHTRAWRFRRIATRYLDPVLRPLAASVPTFAVMTHRGRTSGR
                     VYRTPVNVFRRGDAYLFFLTYGADVDWVKNVLAAGSCSIRTRGHDVALVDPELIEDRE
                     LRAAPLIPRFIERRVAGVDAYLRMREKTQP"
     gene            complement(1433..1675)
                     /locus_tag="VIH70_07150"
     CDS             complement(1433..1675)
                     /locus_tag="VIH70_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116173.1"
                     /translation="MGQAADSKVIEIEQARTQLELDLRELEDRLPAPLRSMKSLAGML
                     LGTAVLVFLSLRRFRSKRSASAPAAEVVVRIVREDR"
     gene            complement(1648..2121)
                     /locus_tag="VIH70_07155"
     CDS             complement(1648..2121)
                     /locus_tag="VIH70_07155"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY8116174.1"
                     /translation="MSDPIGGNGGFQRNASNGTVSAAASGPSPPRPKRPLGAVITSAI
                     DGTRILVRKQVELARIEMTEAVSVRAKGAGMMAGAAVLALFALGFVAAAGSVALALVL
                     PAWAANLIVGLVFLAIAGVLALVGRNAIRNAPTTPERTQETLKEDARWARQQIAR"
     gene            2395..2724
                     /locus_tag="VIH70_07160"
     CDS             2395..2724
                     /locus_tag="VIH70_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116175.1"
                     /translation="MIGLLILGGVIAAIAVIKRRSGSGVDEWDSFAADDVYARASTTT
                     ANTADAVKDAAAKASDAAREAATTATKAAKDAAAKATDAARDAATTASKAAKDAAAKA
                     TDAAKDA"
     gene            2750..3241
                     /locus_tag="VIH70_07165"
     CDS             2750..3241
                     /locus_tag="VIH70_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116176.1"
                     /translation="MSHQAARRAGPAYWISRALILFVYGFAIACIVILAMAFFLQLFN
                     ANTTAPFVDWVYRAAKRIMLPFRGIFPSVEGEQGSVFDVSMLFAMFMYGLLAVGAHGL
                     IDWIDRKIAAKRYEAMWQPAATPAASSGVVTIPTTAQATAPAASSTAPAGTTPPPSPP
                     PSG"
     gene            3593..4303
                     /locus_tag="VIH70_07170"
     CDS             3593..4303
                     /locus_tag="VIH70_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116177.1"
                     /translation="MHTTWSDGTLPLEDMVEASRSLGRPFVAITDHSQALFIANGMTP
                     AELAEQARVIDALNRGSEEDGDAFRVLRSMEVDLFVDGTLDMDDDSLASLDLVLGAFH
                     SELRSKDDQTDRYLAALNQPRLHVLAHPKARMYGRRVGLVADWRRLFAEAARLGKALE
                     LDATVWRQDLNVELATLAREEGVEWFTIGSDAHAARELELLPFGMAIAALAGIPRERI
                     LNYRPLEFVRRWARRSQG"
     gene            complement(4340..>5665)
                     /locus_tag="VIH70_07175"
     CDS             complement(4340..>5665)
                     /locus_tag="VIH70_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020109782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8116178.1"
                     /translation="QFICSFAGSNMNVMITDISTDLDTTVQGVQIAITLFLLVMAALM
                     IPGGKLTDRWGRKRCFTIGLALYGIGALMSAASPGLGILILGNSIFGGVGTALLIPPV
                     YILTTLLFSDITSRARAFGIITGMGGVGAAAGPLIGGLITTAISWRAAFVFQAIVVAV
                     ILWRSRDIVDPVAPDPTRPFDTLGAVLSAAGLILVVMGVLAADNDLALTAVLLAVGSV
                     LLAVFFLHIRARERRHQEPLLSTGLFRNRTSNLGLVTQNVQWLMLIGTAFVVSAYLQV
                     VRGYNAIQTGVIFTAATGGVLLSSLAAERLAKKYPQRTLIIAGFVTTIAGVLVLLALV
                     AGRPSAWAFAPGLLLIGLGVGVMLTPSVNVVQSAFPEEQQGEISGLSRSISNLGSSLG
                     TAVAGTILVSGLTNRSYAASMIVLAIIGIVGLGAAFSLPTQRVQQTLDV"
BASE COUNT          930 a         1894 c         2008 g          833 t
ORIGIN      
        1 ggagaaccag agatggcggg cgtccgaaag ccgaaggtcg acaagtcggg ccgcaccagc
       61 gccggcatcc tgttgtggcg atcgcgaggg ggtcgcctcg aggtgctgct cggccacccc
      121 gggggtccgt acttcgcggg caaggacgcg gatcactgga ccgtgctcaa gggcgaggtg
      181 gatccgggcg aggacctgct ggccttcgcc cggcgcgagt tcgaggagga gacgggattt
      241 cccccgccgg agggtccgac gatcgagctc ggcgagatcc gtcagaagtc cggcaagcgc
      301 gtcctggcct gggcggtggg gggagatctc gacacccaga cggccgtgag caactccttc
      361 gagatggagt ggcctccccg gtccggacgc atgcgggagt tccccgagat cgatcgcgtc
      421 gagtggttcg gcctggacga tgcccgcggg aagatcaagg cggcacaggc cccgttcctg
      481 gatcgtctcg aacggacgct ggccgaggaa cacgatcagg cgcgataacc gtcaagcatc
      541 ccgagccgcg tccgtctcga tcacgccgta gacgttgccg tccggcgccc ggaagtgcgt
      601 ccacgtgaga cccgcctcgt ccgaccggac ggggccgagc aggatcggga cgccggcagc
      661 gcggagctcc tcggtcgctg ccgccacgtc ctcgaccagg aactccacca ccggtccggt
      721 cgtgaggtgg tcgttctgcg tgggtccgaa cacctcggcg atgccgccgt ccggcagcct
      781 gaaggcccac atgtcgtcac cgccgtgcat gggccgaagc gcgagggtac gctcgaacaa
      841 gtcgaccatg gccgcgggct gggccgtgcg ggtaccgacg aaaccgatct tgagcacctt
      901 cacgatctac ctcccttctc gacgagccct gcgattttcg tccacggaca agatcctcat
      961 ggctgcgtct tctcgcgcat ccgcaggtag gcgtcgacgc cggcgacgcg gcgctcgatg
     1021 aagcgaggga tgagcggcgc ggcgcggagc tctcggtcct cgatcagctc cggatccacg
     1081 agcgccacgt cgtgtccccg tgtgcgaatt gaacagctcc cagcagccag gacgttcttc
     1141 acccaatcga cgtcggctcc gtaggtgagg aagaacaggt acgcgtcccc acgacggaac
     1201 acgttgaccg gcgtgcgata cacccgtccg ctcgtgcggc cacggtgcgt catgaccgcg
     1261 aacgtcggta ccgacgcggc cagcgggcgc aggaccggat cgaggtaccg cgtcgcgatt
     1321 cgacggaagc gccaggcacg cgtgtgggcc ttcatcacgg tcgtgagtcc cggtcagccc
     1381 tctcctgcag cccaggcgac cgcctgctcg caatcggccg aagcatgatg gctcagcgat
     1441 cctcgcgcac gatccgcacg accacctcgg cggcgggagc gctcgccgat cgcttcgacc
     1501 ggaaacgcct cagcgagagg aagaccagga ccgccgtccc gaggagcatg ccggccaggc
     1561 tcttcatcga gcggagcggg gcgggcaggc ggtcctcgag ctcccggagg tcgagctcca
     1621 gctgtgtgcg ggcctgttcg atctcgatca ccttgctatc tgctgcctgg cccatcgggc
     1681 gtcctccttc agcgtctctt gcgtccgctc cggggtcgtt ggagcgttcc ggatggcatt
     1741 gcggccgacc agggcgagca cacccgcgat cgcgaggaac acgagcccga cgatcaggtt
     1801 ggccgcccag gccgggagca ccagggcgag cgcgaccgat cccgccgccg cgacgaagcc
     1861 gagcgcgaac agggccagca ctgcggcccc cgccatcatg ccggccccct tcgcgcgaac
     1921 cgacacggcc tcggtcatct cgatcctggc cagctcgacc tgcttgcgca ccaggatgcg
     1981 ggtgccgtcg atggccgagg tgatcacggc gcccagcggc cgcttcggcc gcggcggcga
     2041 tgggcccgat gcggcggcac tgaccgttcc gttcgaggcg ttccgttgga agcccccgtt
     2101 ccccccgatc gggtcgctca cggtcacgat ccctcccttc gcgctccgcg agcgatccgc
     2161 tccgggcgcc gtccgccgtc ccgccggact cgggctgggg ccgagcccat cgtgcgaagg
     2221 cgctcgcccg ccaggatcca tgcgtacacc atctcgcccg gacgcgcacg gtcgatcctc
     2281 gcgacgggtg tgtgatcggc cgcgcgcccg gggcgggcgg ggccggtggc gatctgactt
     2341 ccgatgacgt taggctgagg ggccacgggg caggaggtga ccatgaggaa actcttgatc
     2401 gggctgctga tcctcggtgg tgtgatcgcc gccatcgccg tcatcaagcg ccgctcgggc
     2461 tcgggcgtcg acgaatggga ctcgttcgcc gccgatgacg tgtacgcgcg ggcgtccacg
     2521 accaccgcga acaccgcaga cgccgtgaag gacgccgccg cgaaggccag cgacgccgcg
     2581 agggaagccg ccacaacagc cacgaaggct gcgaaggacg ccgccgcgaa ggccaccgat
     2641 gccgcgaggg acgccgccac gacagcctcg aaggctgcga aggacgcggc tgcgaaggcc
     2701 accgacgctg cgaaggacgc ctgagcacgt cgagacgaag gaggcccgca tgtcccatca
     2761 agccgcccgc cgggcgggtc ccgcctactg gatcagccgt gcgctgatcc tgttcgtcta
     2821 tggcttcgcg atcgcgtgca tcgtcatcct cgcgatggcg ttcttcctcc agctcttcaa
     2881 cgcgaatacc acggccccct tcgtcgactg ggtctaccgg gcggccaaga ggatcatgct
     2941 gccgttccgc gggatcttcc cctccgtcga gggcgaacag ggctcggtct tcgacgtttc
     3001 gatgctcttc gccatgttca tgtatgggct gctggcggtg ggtgcgcatg ggctgatcga
     3061 ttggatcgac cggaagatcg cggccaagcg gtacgaggcg atgtggcagc cggccgccac
     3121 accggccgcg agctcgggtg tcgtgacgat cccgaccacg gctcaggcaa cggctccggc
     3181 cgcgtcctcc accgctccag ccggcacgac gccgccgccg agcccgcctc cgtcggggta
     3241 gaggccgacg cgggacgcat cggccgcatc gccgacgaac ggtgatctcg gcgagctgct
     3301 ccggcgtgct gcgcgtgagg agaccgacca ccgccgcagg gcgctggaac gagcatcgag
     3361 ggcatcgcgg ttctggccgg aggaggcgag ggaggtcgcg ggttccgggc gccagctcac
     3421 ggagctgcgt gccgtcgggc catgggtcgc cgcccggatc gaggcgtggc tcgaggatcc
     3481 tcccgagccc gaatccgacg agacgcggcg tgggttcctc acctacgcgg aggtccgccg
     3541 gacgctcgac gccgatcctt cgtgggagtc caccccgtgc gcggatctcc agatgcacac
     3601 cacatggagc gacggcaccc tcccgctcga ggacatggtg gaggcctcga gatccctggg
     3661 acggccgttc gtggccatca ccgaccactc gcaggcgctc ttcatcgcga acggcatgac
     3721 gcccgccgag ctcgcggagc aggctcgcgt gatcgacgcc ttgaaccgcg ggtccgagga
     3781 ggacggtgac gcgttccgcg tgctccgctc gatggaggtc gacctcttcg tcgacggcac
     3841 gctcgatatg gacgacgact cgctcgcgag cctcgatctg gtgctcggcg cgttccactc
     3901 agagctgcgg tccaaggatg accagaccga ccgctacctc gccgccctca accagccgcg
     3961 cctgcacgtg ctcgcgcacc cgaaggcccg gatgtacggc cgccgggtcg gcctcgtcgc
     4021 cgactggcgc cgcctgttcg ccgaggccgc gaggctgggg aaggcgctgg agctcgacgc
     4081 gacggtgtgg cggcaggatc tgaacgtcga gctcgccacc ctcgcgcgcg aggagggcgt
     4141 cgagtggttc acgatcggga gcgacgccca tgcggcgcgg gagctcgagc tcctcccgtt
     4201 cgggatggcg atcgccgcgc ttgccggcat cccgcgcgag cgcatcctca actaccggcc
     4261 gttggaattc gtccgtcggt gggcgcgccg gtcacagggc tgatccaggc gcttcggtcg
     4321 gcaagcccag cggatgccgt tagacatcga gtgtctgctg aactctctgg gtgggcaggg
     4381 agaaggccgc tccgagcccg acgatcccga tgatcgcgag aacgatcatc gaggcggcgt
     4441 aggaccggtt cgtgaggccg gagacgagga tggtgccggc gaccgcggtg ccgagtgacg
     4501 agcccaggtt ggagatggag cgcgacaggc cggagatctc gccctgctgt tcttcgggga
     4561 aggcggattg cacgacgttg acggaaggag tcagcatcac gccgacaccg aggccgatga
     4621 gcaggagtcc gggcgcgaac gcccaggcgc ttggccggcc cgccacgagg gcgagcagga
     4681 cgaggacccc ggcgatcgtg gtgacgaagc cggcgatgat cagcgtccgc tgcggatact
     4741 tcttggcgag ccgttcggct gcgagcgatg agagcaagac gcctccggtg gcggcggtga
     4801 agatcacgcc ggtctgtatg gcgttgtagc cgcggacgac ctgcagatac gcggagacga
     4861 cgaacgccgt gccgatgagc atgagccatt gcacgttctg ggtgaccagc ccgaggttcg
     4921 acgtgcgatt gcggaacagt ccggtcgaca gcaagggctc ctggtgtcgg cgttctcggg
     4981 cccggatgtg gaggaagaac acggcgagga ggacggagcc gaccgccaac agaaccgccg
     5041 tgagtgcgag gtcgttgtcg gcggcgagga cccccatcac caccaggatg aggccggcgg
     5101 ccgacaagac cgcgccgagt gtgtcgaagg gccgggtcgg gtcgggcgcg acgggatcga
     5161 cgatgtcgcg gctccgccag aggatcacgg cgacgacgat cgcctggaag acgaaagcag
     5221 cccgccagct gatggcggtg gtgatgagtc ccccgatcag cggtcccgcg gcggctccga
     5281 cgccgcccat cccggtgatg atcccgaaag cacgggctcg cgacgtgatg tcgctgaaga
     5341 gcagggtggt caggatgtac acgggtggga tcagcaatgc ggtcccgaca cctccgaaga
     5401 tcgagttgcc gaggatgagg atgccgagac cgggggaggc cgcgctcatc agcgcgccga
     5461 tcccatagag ggcgaggccg atggtgaagc agcgcttgcg gccccatcga tcggtgagct
     5521 tgccaccggg gatcatgaga gcggccatca ccaacaggaa gagggtgatc gcgatctgta
     5581 cgccctgcac ggtcgtgtcg aggtccgtgc tgatgtcggt gatcatgacg ttcatgttcg
     5641 agccggcgaa gctgcagatg aactg
//
LOCUS       DATBRK010000235         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_900580, whole genome shotgun sequence.
ACCESSION   DATBRK010000235 DATBRK010000000
VERSION     DATBRK010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900580"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..1087
                     /locus_tag="VIH70_07180"
     CDS             365..1087
                     /locus_tag="VIH70_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8116179.1"
                     /translation="MTAGQGTENHARRGQIVDVAMRQFAEHGYRGAHVEDIAAEVGVA
                     KGTVFLHFGNKEGLFLAVYREAVGQLPAWLDAPGEIVDAGFWSVLDYWLRRTEEFLTE
                     QWVANRVAMIGRYDTGLGLRRPIDRLMRSEDPYGTLEFVEFGVHRGEIRDDVDVEMIA
                     SMLDWVAERFQDALGSEELDPGLVHRQPHVAGRRDARIREFLELLRSGISPAPSPLAD
                     LDPGASGRPATMEPARPGGTDG"
     gene            1084..1923
                     /gene="proC"
                     /locus_tag="VIH70_07185"
     CDS             1084..1923
                     /gene="proC"
                     /locus_tag="VIH70_07185"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY8116180.1"
                     /translation="MSATHDRKVAFLGGGRMGEAFVSGLIRSGGRRVDEIMVTSRRDE
                     RSRELAETYGIASTLSNPDAVAWADVLVLTVKPQDMEALLSQIREHVTHEHLVVSFAA
                     GIRTSFVEKHLPDGVPVVRVMSNVAVLVDEAMSVVAAGSNAEDRHLEVAEELLGYVGR
                     VIRLKEVHLDAITATSGSGPAYFFLLAEAMIEACILLGLSRDVATELIIQTMLGSAKM
                     LRDTGKHPVELREMVTSPGGTTIAAIRHLEEAGVRAAFLNAIDAARHRSAELAQGGDE
                     DQD"
     gene            complement(2108..>2792)
                     /locus_tag="VIH70_07190"
     CDS             complement(2108..>2792)
                     /locus_tag="VIH70_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8116181.1"
                     /translation="AFPETMERLVRSNGGTVLDAARAALERDEADDFVVERPRLLAAL
                     LPTALRSTDAVEGGRPAFGPDEEIPWPEGAGWLEFHPKIAAVLRRGVDDAITPEEAPS
                     IVFGYTLVGDWLARRANGDPMPTPRGVPLSIGPCVVTADELDPQTAFVTVRIDGEEWV
                     KGNLNGTALGLLRDVARASKQERLESGETFASGPFEIPGFEQRVWPGAEVELEAEGIG
                     ILRNRLGQM"
BASE COUNT          438 a          964 c          996 g          394 t
ORIGIN      
        1 cgccggtaca tttcgctcgc tccgagcagc cggagcggcc gaatcaggga accgaggccg
       61 ggacccctgg gaaagccgta aggccgactg ggcgcacctg ctcgtacggt tgggacagct
      121 aactagctgt gcgggtgggg aggctcggga ggccccaaag cccaacgaaa gggtactacg
      181 gccaggaaac caagacggct gtgagaggtt gctcggagca cctgagcccg aggggccaac
      241 ggtggctgga ggcttcggct tccggcgagc cggaggtccc tcgggttctt ctttgtcctt
      301 ccgcccagac accccgcgac gctggcttgt tcgcccggcg gggcgcggct accgttccca
      361 ggtcatgacc gccggacagg gaaccgagaa ccacgcccga cgcgggcaga tcgtggacgt
      421 cgcgatgcgg caattcgccg aacacggcta tcgcggtgcg cacgtcgagg acatcgccgc
      481 cgaggtgggg gtcgccaagg ggacggtctt cctgcacttc gggaacaagg aggggctgtt
      541 cctcgcggtg tatcgggagg cggtcgggca gctcccggcc tggctcgatg ccccagggga
      601 gatcgtggat gccgggttct ggtccgtcct ggactactgg ctccgccgaa ccgaggagtt
      661 cctcaccgag caatgggtcg ccaaccgcgt cgcgatgatc gggcggtacg acaccgggct
      721 cgggctccgg cgcccgatcg atcggctcat gcggagcgag gatccctacg ggacgctcga
      781 gttcgtcgag ttcggggtgc atcgcggcga gatccgggac gacgtcgacg tggagatgat
      841 cgcctcgatg ctggactggg tcgccgaacg gttccaggac gccctcggca gcgaggagct
      901 cgaccccggg ctggtccacc gccagcctca cgtcgccggg cggcgcgacg cacggatccg
      961 cgagttcctc gagctcctgc gctcgggcat ctcaccggca ccgagcccgc tcgcggatct
     1021 cgaccccggc gcgtccggac ggccggcgac gatggagccc gctcgccccg ggggaaccga
     1081 cggatgagcg cgacccacga tcgcaaggtg gcgttcctcg gcggcggccg gatgggcgag
     1141 gccttcgtgt ccgggctgat ccgctccggc ggccgcaggg tggacgagat catggtcacg
     1201 agccgccgcg acgagcgctc gcgggagctc gcggagacct acgggatcgc gtcgacgctc
     1261 tcgaacccgg acgcggtcgc ctgggccgac gtcctggtcc tcacggtgaa gccgcaggac
     1321 atggaggccc tgttgtcaca gatccgcgag cacgtgaccc acgagcacct ggtcgtgagc
     1381 ttcgccgccg ggatccggac ctcgttcgtc gagaagcacc tgcccgatgg cgtcccggtc
     1441 gtccgcgtga tgtcgaacgt ggccgtcctc gtggacgagg ccatgtcggt cgtcgccgcg
     1501 ggctcgaacg ccgaggaccg gcacctcgag gtggccgagg agctcctcgg gtacgtcggc
     1561 agggtgatcc ggttgaagga ggtccacctc gacgcgatca cggccaccag cggcagcggc
     1621 cccgcctact tcttcctgct cgccgaggcg atgatcgagg cctgcatcct gctgggactc
     1681 tcgagggacg tcgcgaccga gctcatcatc cagacgatgc tggggtccgc caagatgctc
     1741 cgcgacaccg gcaagcatcc ggtcgagctg cgtgagatgg tgacctcgcc gggcgggacg
     1801 accatcgccg cgatccggca tctcgaagag gcgggcgtac gtgccgcctt cctgaacgcg
     1861 atcgacgccg cccggcatcg aagcgccgag ctcgcgcagg gtggcgacga ggaccaggac
     1921 tgaggagaac gatgggtcga ggagatcgag cgagggtgcg ctgggcgcac gatcgcgaac
     1981 ggaagaggaa agccgcggag aaacgtcagg ccgccgagcg cggggccgaa cgcaagcgcg
     2041 cgaagaagta ggtcaaggcg cccggacggt tgcgatccac gccgctcgag ccgggcgacg
     2101 atcgcgtcta catctgtccg agccggttcc gcaggatccc gatcccctcg gcctcgagct
     2161 cgacctcggc gccgggccac acgcgctgct cgaaccccgg gatctcgaac gggcccgagg
     2221 cgaacgtctc gcccgattcc agccgctcct gcttcgacgc ccgggcgacg tcgcggagca
     2281 ggccgagcgc cgtgccgttc aggttcccct tgacccactc ctcgccgtcg atcctgaccg
     2341 tgacgaacgc ggtctgggga tcgagctcgt ccgccgtcac cacgcacggg ccgatcgaga
     2401 gcgggacgcc gcgtggggtc ggcatcggat cgccgttcgc gcgccgcgcg agccagtcac
     2461 ccacgagcgt gtagccgaag acgatcgaag gcgcctcctc gggcgtgatc gcgtcgtcca
     2521 ccccgcgccg caggacggcc gcgatcttcg gatggaactc gagccagccc gccccctccg
     2581 gccacgggat ctcctcgtcc gggccgaacg ccggccgccc gccctcgacg gcgtccgtcg
     2641 aacgcagcgc cgtcggcagg agcgccgcga gcagacgtgg tcgttcgacg acgaagtcat
     2701 ctgcctcgtc gcgctcgagg gctgcccggg cggcatcgag caccgttccc ccgttgctcc
     2761 tgacgagccg ctccatcgtc tccgggaacg cg
//
LOCUS       DATBRK010000236         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_473550, whole genome shotgun sequence.
ACCESSION   DATBRK010000236 DATBRK010000000
VERSION     DATBRK010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473550"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="groEL"
                     /locus_tag="VIH70_07195"
     CDS             complement(<1..1067)
                     /gene="groEL"
                     /locus_tag="VIH70_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8116182.1"
                     /translation="MASKLLKFSEDARRALETGVNKLADTVALTLGPKGHNVVLDKKW
                     GAPTITNDGVTIAKEVELEDPWENMGAQLAKEVATKTNDVAGDGTTTATVLARAMVHR
                     GMRNVAAGANPMALKRGIEKAVVVAVDAIKDQAKDVGSREDISNVAAISAADPTIGNV
                     LAEALDKVGKDGVVTVEESNTFGMELEFVEGMQFDKGYISPYFVTDQERMEAVLEEPY
                     IALVNKKIGGVQELLPLLEKVLQAGRALLLIAEDVEGEALATLVVNKIRGTFNVVGVK
                     APGFGDRRKAMLQDIATLAGGQVISEEVGLKLENVGLDLLGSARKVVVTKDDTTIVEG
                     GGGQDEVKGRINQIKAEIDKT"
     gene            complement(1147..1449)
                     /gene="groES"
                     /locus_tag="VIH70_07200"
     CDS             complement(1147..1449)
                     /gene="groES"
                     /locus_tag="VIH70_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY8116183.1"
                     /translation="MAKKDFDLKPLEDRIVVKPGDEEEMTVSGIVIPDTAKEKPQEGS
                     VVAVGPGRFEEGARVPLDVAVGDTVIYSKYGGTEVKVAGEEYLILSARDVLAIISK"
BASE COUNT          212 a          552 c          468 g          299 t
ORIGIN      
        1 tcggtcttgt cgatctccgc cttgatctgg ttgatgcgtc ccttgacctc gtcctgaccg
       61 cctccgccct cgacgatggt ggtgtcgtcc ttggtcacga cgaccttcct cgcgctgccg
      121 agcaggtcga gcccgacgtt ctcgagcttc agtccgacct cctcgctgat gacctgtcca
      181 ccggcgaggg tcgcgatgtc ctggagcatc gccttgcggc ggtcgccgaa cccgggcgcc
      241 ttgaccccga ccacgttgaa cgtgccgcgg atcttgttga cgaccagcgt ggcgagcgcc
      301 tcgccctcga cgtcctcggc gatcagcagg agcgcccggc cggcctggag caccttctcc
      361 agcagcggga gcagctcctg gacgccgccg atcttcttgt tcacaagggc gatgtacggc
      421 tcctcgagga ccgcctccat ccgctcctgg tcggtgacga aatacggcga gatgtagccc
      481 ttgtcgaact gcatgccctc cacgaactca agctccatgc cgaacgtgtt cgactcctcc
      541 accgtcacga ccccgtcctt accgaccttg tcgagcgcct cggcgagcac gttgccgatc
      601 gttgggtcgg ccgccgagat cgcggccacg ttggagatgt cctcgcgcga acccacgtcc
      661 ttggcctggt ccttgatcgc gtccacggcc acgacgacgg ccttctcgat gccgcgcttg
      721 agcgccatcg ggttcgcgcc ggccgcgacg ttgcgcatgc cgcggtggac catcgcccgg
      781 gccagcacgg tcgcggtcgt ggtgccgtcc ccggccacgt cgttggtctt ggtggcgacc
      841 tccttcgcga gctgggcgcc catgttctcc caagggtcct cgagctcgac ctccttggcg
      901 atcgtgacgc cgtcgttcgt gatcgtcggc gcaccccact tcttatccag gaccacgttg
      961 tggcccttcg gtccgagcgt gagtgcgacg gtgtcggcca gcttgttcac gccggtctcc
     1021 agagcgcgac gggcgtcctc gctgaacttc agcagcttgc ttgccatggg acctcccttg
     1081 atgacgggac agagacggga cgggggccga gccatcacgg cccgcccccg cccgaacgcc
     1141 ttctagctac ttcgagatga tggccagcac gtcccgggcc gacaggatca ggtactcctc
     1201 gcccgcgacc ttgacctcgg tgccgccgta cttcgagtag atgacggtgt cgcccacggc
     1261 gacgtcgagc ggaacccgcg ccccctcctc gaaacgtccc ggaccgaccg ccacgaccga
     1321 gccctcctgg ggcttctcct tcgcggtgtc cgggatcacg atcccggaga cggtcatctc
     1381 ctcctcgtcg ccgggcttca cgacgatgcg atcctccagg ggcttcaggt cgaagtcctt
     1441 cttggccatg cgttcccctc cttgcgcgtt agcactcgac tacggtgagt gctaagagga
     1501 tatcggggcg agtgccgagg gtcaacgggg g
//
LOCUS       DATBRK010000237         4335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2494273, whole genome shotgun sequence.
ACCESSION   DATBRK010000237 DATBRK010000000
VERSION     DATBRK010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2494273"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VIH70_07205"
     CDS             complement(<1..364)
                     /locus_tag="VIH70_07205"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY8116184.1"
                     /translation="MSIQVLPVRGLPEVRPGGDLAALLAGPLGTIGLDDGDVVAVTQK
                     IVSKAEGRVVREEGRGRDAWVEHETHRVVARRGDLVIAETRHGFVCANAGVDASNVEE
                     GFLTLLPEDPDASAERLRR"
     gene            complement(385..1326)
                     /gene="cofD"
                     /locus_tag="VIH70_07210"
     CDS             complement(385..1326)
                     /gene="cofD"
                     /locus_tag="VIH70_07210"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEY8116185.1"
                     /translation="MRVTALAGGVGAGKFLRGLVRAVAPDAVTVIANTGDDITMHGLN
                     VSPDLDSVTYWLGDVFDRERGWGRRDETFRATEELRSFDPELAWFNLGDLDLATHLFR
                     TNLRAAGETLTSATAAIARRFGVVPRILPMSDDPVTTRISVVADGEELDLHFQEYWVR
                     RGGRDEVKAVRYEGAERSRPAPGVLEALADADAIVLCPSNPVASLGPILAVPGIRDTI
                     GARRTDVVGVSGIVAGAPLAGMADRLMPAAGAEVTAAGAAEFYRGLLGAWVVDDLDRA
                     LIPRIEATGLRVGATDSIMTDDAKAEALARFALGLRG"
     gene            1356..3917
                     /locus_tag="VIH70_07215"
     CDS             1356..3917
                     /locus_tag="VIH70_07215"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF006687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HEY8116186.1"
                     /translation="MAEATTAHPLRRALARAGAGKALSVDEIEALLGARGPDLGRLMS
                     LASHLRELGHGGTVTYSRKVFVPLTMLCRDHCHYCTFAKPPARLERPFLSPEEVVAIA
                     EAGRRLGCKEALFTLGDRPEERYPVARAWLAERGYRSTLEYVRAAAIRVIEETGLLPH
                     LNPGVMSYEEMARLKQVAPSMGLMLETSSDRLSERGGPHFRSPDKVPAVRLRVIEDAG
                     RLAIPFTTGILVGIGETLAERAASLLAIRELHRRYRHVQEVIVQNFRAKPGTAMHDAP
                     EPGDEEFLATVATARVVFGPRMHVQAPPNLSDPEQQTRLLDSGIDDWGGVSPLTPDHV
                     NPERPWPAIEELHRRTAERGLELRERLTIYPEFALRPDPFLAGKMRAPVSAVMGSDGL
                     AAEMLAAPIGWQDPEVAWKPRSTELTFAKVQGAGPRADAEVVYGETDVPERTLAWHAE
                     RVPPRRLAAEIRSGLAKALAHRPLTDGEALALFRADGDDLEALCAVADDLRREAVGED
                     VTYVVNRNINFTNVCYVGCRFCAFAQREIDPESYTLTLSEVAERAREAVAWGCTEVCM
                     QGGIHPDLPGSFYFDLLDAVKGAAPELHIHAFSPMEVLNGATRLGISFREFLQECRRH
                     GLGTIPGTAAEILDDDVRWVLTKGKLPADAWEEIVRTAHDLGIRSSSTIMFGHVDAPP
                     HWVFHIRRLARIQRDTGGFTEFVPLPFVHQNAPIYLAGKARPGPSFDDNRRMHAVARV
                     LLDGVIHNVQVSWVKMGVEACQTMLRGGANDFGGTLMEETISRMAGAEWGIRMEPAEI
                     RTAITSIGRTPVERSTTYERLQRNANWHVRHAPVTRLGAAVAGRTPSDARFRQGD"
     gene            4108..4335
                     /locus_tag="VIH70_07220"
     CDS             4108..4335
                     /locus_tag="VIH70_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY8116187.1"
                     /translation="MHHYIPWQERARCRQHDPEVFFPEKGGSSREAKRICADCPVRIE
                     CLNYALRRDERYGVWGGMSERERRRLKRMAS"
BASE COUNT          665 a         1536 c         1518 g          616 t
ORIGIN      
        1 cgcggcggag ccgttccgcg gaggcgtccg ggtcctccgg gagcagcgtg aggaacccct
       61 cctcgacgtt cgacgcgtcg acgccggcgt tcgcgcacac gaagccgtgg cgcgtctcgg
      121 cgatcacgag gtcgccgcgc cgggcgacga cgcggtgcgt ctcgtgttcg acccacgcgt
      181 cgcggccgcg tccctcctcc cggacgacgc gtccctcggc cttcgacacg atcttctgcg
      241 tgaccgcgac cacgtccccg tcgtcgagcc cgatggtgcc gagcggtccg gcgaggagcg
      301 cggcgagatc gccgcccggt cgcacctccg ggagcccccg gactggcagc acctggatgc
      361 tcatgcggga ggggtcgccc cccatcaccc ccgcagaccg agcgcgaacc gcgcgagcgc
      421 ctccgccttc gcgtcgtcgg tcatgatcga gtccgtcgcg ccgacacgta gcccggtggc
      481 ctcgatccga gggatcagcg cgcggtcgag gtcgtcgacg acccaggcgc ccaggagccc
      541 ccggtagaac tccgcggcac cggccgcggt cacctcggcg ccggccgccg gcatcagccg
      601 gtccgccatc cccgcgagcg gcgcgcccgc aacgatcccg ctcacgccga cgacgtcggt
      661 gcgacgcgcc ccgatggtgt cgcggatgcc cggcaccgcg aggatcggac cgagcgaggc
      721 gaccgggttc gacgggcaca gcacgatcgc gtccgcgtcc gcgagcgcct cgaggacgcc
      781 gggcgcgggt cgcgagcgtt cggccccctc gtaccgaacg gccttcacct cgtcgcgtcc
      841 gccgcgacgg acccagtact cctggaagtg caggtcgagc tcctcgccgt cggcgaccac
      901 tgagatccgc gtggtcaccg ggtcgtcgct catcgggagg atccgcggca ccacgccgaa
      961 ccgccgcgcg atggcggccg tcgccgaggt cagcgtctcg cccgccgccc ggaggttcgt
     1021 ccggaacagg tgggtggcga ggtcgaggtc gccgaggttg aaccacgcca gctccggatc
     1081 gaacgatcgc agctcctcgg tggcgcggaa ggtctcgtcg cggcgacccc atccgcgttc
     1141 gcggtcgaag acgtcgccga gccagtacgt caccgagtcg agatcgggtg acacgttcag
     1201 cccgtgcatc gtgatgtcgt cgccggtgtt cgcgatcacg gtgaccgcgt ccggcgcaac
     1261 ggcgcgcacg agcccacgca ggaacttccc cgcgccgacc ccgccggcga gcgccgtcac
     1321 ccgcatccac ggcagcgtag tacgctcgcg aacggatggc cgaagccacg accgcgcacc
     1381 cgcttcggcg cgcgctcgct cgcgcgggcg ccggcaaggc gctgtcggtc gacgagatcg
     1441 aggcgctgct cggggcgcgc ggtccggacc tgggacggct gatgtccctc gcgtcgcacc
     1501 tgcgcgagct gggacacggc ggcaccgtga cgtactcccg caaggtcttc gtcccgctca
     1561 cgatgctgtg tcgcgaccac tgtcattact gcacgttcgc caagccgccc gccaggctcg
     1621 aacggccgtt cctgtcgccc gaggaggtcg tcgcgatcgc cgaggcgggg cggcgtctcg
     1681 ggtgcaagga ggcgctgttc acgctcggcg atcggccgga ggagcgctac ccggtggcgc
     1741 gcgcgtggct cgccgaacgc gggtaccgct cgacgctgga gtacgtccga gcggccgcga
     1801 tccgagtgat cgaggagacg gggctgctcc cgcacctcaa tcccggggtc atgtcctacg
     1861 aggagatggc gcggctcaag caggtcgctc cctcgatggg gctgatgctc gagacatcct
     1921 ccgaccgcct gtccgaacgc ggcgggccgc acttccgctc gccggacaag gtcccggccg
     1981 tccggctccg tgtgatcgag gacgccgggc gtctggcgat cccgttcacg acgggcatcc
     2041 tcgtcgggat cggcgagacc ctcgccgagc gcgcagcgtc gctgctcgcg atccgcgagc
     2101 tgcatcggcg gtaccggcac gtccaggagg tcatcgtcca gaacttccgg gccaagcccg
     2161 gaaccgcgat gcacgacgcg cccgagccgg gcgacgagga gttcctggcc accgttgcga
     2221 ccgcgcgggt cgtcttcggg ccgcggatgc acgtccaggc gccgccgaac ctgtccgacc
     2281 cggagcagca gacccgactg ctggactcgg ggatcgacga ttggggcggg gtctccccgc
     2341 tcacgccaga ccacgtgaac cccgagcgcc cgtggccggc gatcgaggag ctccaccgtc
     2401 gcacggccga gcgcgggctc gaactgcggg agcggctcac gatctacccg gagttcgcgc
     2461 tccgacccga tccgttcctc gcgggcaaga tgcgcgctcc cgtctccgcc gtgatggggt
     2521 cggacggcct ggccgcggag atgctcgcgg cgccgatcgg gtggcaggac cccgaggtcg
     2581 cctggaaacc ccggtcgacc gagctcacgt tcgcgaaggt ccagggcgcg ggcccgcggg
     2641 cggacgccga ggtggtctac ggcgagaccg acgtgcccga acgcaccctg gcgtggcatg
     2701 ccgaacgcgt gcccccgcgc cggctcgccg ccgagatccg gtcggggctc gccaaggccc
     2761 tagcgcatcg gccgctgacc gacggggagg ccctcgcgct gttccgggcg gacggggacg
     2821 acctcgaggc cctgtgcgcg gtggccgacg acctgcggcg cgaggcggtg ggcgaggacg
     2881 tcacctacgt cgtgaaccgc aacatcaact tcacgaacgt ctgctacgtc gggtgccggt
     2941 tctgcgcttt cgcccagcgc gagatcgacc cggagtccta cacgctcacg ctctcggagg
     3001 tggccgaacg cgccagggag gccgtcgcgt gggggtgcac cgaggtgtgc atgcagggcg
     3061 ggatccaccc cgatctgccg ggttcgttct acttcgacct gctcgacgcc gtgaagggcg
     3121 ccgcgccgga gctccacatc cacgcgttca gcccgatgga ggtgttgaac ggcgccacca
     3181 ggctcgggat ctcgttccgc gaattcctgc aggagtgtcg tcgccacggt ctggggacga
     3241 tcccggggac cgcggccgag atcttggacg acgacgtccg atgggtgctg accaagggca
     3301 agctgcccgc cgacgcatgg gaggagatcg tccgaaccgc ccacgacctc gggatccgca
     3361 gctcatcgac gatcatgttc gggcacgtcg acgcgccccc gcattgggtt ttccacatcc
     3421 ggcggctcgc ccggatccaa cgcgacacgg gcggattcac cgagttcgtc ccgctcccgt
     3481 tcgtgcacca gaacgcgccg atctacctcg ccggcaaggc gcgacccggt ccgtcgttcg
     3541 acgacaaccg gcggatgcac gccgtcgcga gggtcctgct tgacggagtt atccacaacg
     3601 ttcaggtctc gtgggtgaag atgggcgtcg aagcgtgtca gacgatgctg aggggcggag
     3661 cgaacgactt cggcggcacg ctgatggaag aaacgatcag ccggatggcc ggcgcagagt
     3721 gggggatccg catggagcct gccgagatcc gcaccgcgat cacgtcgatc ggccgcacgc
     3781 ccgtcgaacg ttcgaccacg tacgaacgct tgcagcgcaa tgcgaactgg cacgtccgcc
     3841 acgcaccggt gacacggctg ggggccgcgg tcgccgggcg aacaccctcc gatgcccgtt
     3901 tccgtcaagg agattgaccg aaccggtcaa tgagatagac catcgaaata gttcgcacaa
     3961 ccccttgcac ccgcgcccaa gcacgtattc gattgttctg gggacgcatg tggagatgtc
     4021 cgagaactcg agtttccctc ttgccaagag gggagcagat ggcccagaat cacacgcgtg
     4081 tgatttcggg gagggaacgg ggaccacatg catcactaca tcccatggca ggagcgggca
     4141 cggtgtcggc aacacgatcc ggaggtgttc ttccccgaga aggggggttc atcgagagag
     4201 gccaagcgga tctgcgccga ttgtccggtt cgcatcgagt gcctcaacta cgcccttcgc
     4261 cgcgacgagc ggtacggggt gtggggaggg atgagcgaac gcgaacggcg acgcctgaag
     4321 cgcatggcgt cctga
//
LOCUS       DATBRK010000238         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2202335, whole genome shotgun sequence.
ACCESSION   DATBRK010000238 DATBRK010000000
VERSION     DATBRK010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202335"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VIH70_07225"
     CDS             complement(<1..537)
                     /locus_tag="VIH70_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY8116188.1"
                     /translation="MNLIRTGARGEDVRDVQHRLLGAGLRIEPDELDGSFGDSTEAAV
                     REFQRARGLPPDGIIGPDTWSQLVETGYRLSDRTLYLRSPAFRGDDVRELQRMLNALG
                     FDAGKEDGIFGPRTDSGVREFQRNVASRVDGIVGLDTVHAIGRYRPPVDAPSRAVVRE
                     GEAARGPGLALAGSTIAID"
     gene            complement(594..920)
                     /gene="trxA"
                     /locus_tag="VIH70_07230"
     CDS             complement(594..920)
                     /gene="trxA"
                     /locus_tag="VIH70_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY8116189.1"
                     /translation="MMGDVTDADFEERVLGSQSVVLVDFWAEWCVPCHMVSPVVEEIG
                     RDKGEALQVAKLNIDDNPDVTRRFGVMSIPTLMLFKDGQEVARVVGARGKDALLREID
                     PHVPAA"
     gene            complement(972..>1841)
                     /gene="trxB"
                     /locus_tag="VIH70_07235"
     CDS             complement(972..>1841)
                     /gene="trxB"
                     /locus_tag="VIH70_07235"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY8116190.1"
                     /translation="ARANLRPVLVKGLEAGGQLMLTTDVENYPGFPDGILGPEMMDAF
                     EKQAARFETEIVAQTVTRVDLSERPFGVWAGDLEWRAQTLILATGASARWLGVPGEET
                     LRGRGVSACATCDGFFFRDRELVVVGGGDTAMEEATFLTKFASSVTIVHRRDEFRASK
                     IMQERALSNPKIRVIWDAVVEEILGDGAVAGVRLRNVRTDETSELPTGGVFVAIGHTP
                     NTDLVKGQLELTDAGYVVVQEPTTRTSVPGVFAAGDVTDLIYRQAVTAAGQGCKAAID
                     AERFLEERAHRTG"
BASE COUNT          252 a          696 c          604 g          289 t
ORIGIN      
        1 gtcgatcgcg atggtcgagc cggcgagcgc cagcccgggt ccccgcgccg cctcgccctc
       61 gcgcacgacg gcgcggctcg gcgcgtcgac cggcggccgg tagcggccga tcgcgtgcac
      121 cgtgtcgagc ccgacgatcc cgtccacccg ggacgcgacg ttgcgttgga actcgcgaac
      181 gccgctgtcg gtgcgcggcc cgaagatccc gtcctctttg ccggcgtcga agccgagggc
      241 gttcagcatc cgctggagct cgcggacgtc gtcgccgcgg aacgccggcg agcgcaggta
      301 cagggtgcgg tcgctgagcc ggtagcccgt ctcgacgagc tggctccagg tgtccgggcc
      361 gatgatcccg tccggcggga gtccgcgggc ccgctggaac tcacggacgg ccgcctcggt
      421 ggagtcgccg aacgacccgt cgagctcgtc gggctcgatc cgcagccccg cgccgagcag
      481 ccggtgctgc acgtcgcgca cgtcctcgcc tcgcgcaccc gttcggatga ggttcatcac
      541 cggagtctcc gtggaaccga ggccgtccgg caactacgga cgggtgcccc gcgctaggcg
      601 gcggggacgt gcgggtcgat ctcccgcagg agcgcgtcct tgcctcgggc gcccacgacg
      661 cgggcgacct cctggccgtc cttgaacagc atcagcgtcg ggatgctcat caccccgaag
      721 cgtcgtgtga cgtctgggtt gtcgtcgatg ttcagcttgg ccacctggag cgcctcgccc
      781 ttgtcccgac cgatctcttc gaccaccggc gagaccatgt ggcaggggac gcaccactcg
      841 gcccagaagt ccacgagcac cacgctctgc gagccgagca cacgctcctc gaaatcggcg
      901 tccgtcacgt cacccatcat cgccacgtca atccgtcctt tcgaaactcc gtcgtgtcct
      961 cgtcgtaccg ttcaacccgt tcggtgcgcc cgctcttcca ggaaccgctc cgcgtcgatg
     1021 gccgccttgc atccctgtcc ggcggccgtg accgcctgac ggtagatcag atcggtgacg
     1081 tcaccggccg cgaacacgcc ggggacgctc gtccgtgtgg tcggctcctg cacgacgacg
     1141 tagccggcgt cggtgagctc gagctgtccc ttgacgaggt ccgtgttcgg ggtgtgcccg
     1201 atcgcgacga acacgccgcc ggtcggcagc tcgctcgtct cgtccgtccg cacgttccgc
     1261 aggcgcacgc cggccaccgc gccgtcgccg aggatctcct cgacgaccgc gtcccagatc
     1321 acgcggatct tcgggttcga cagcgcccgc tcctgcatga tcttcgacgc gcggaactcg
     1381 tcgcgacggt gcacgatcgt gaccgagctc gcgaacttcg tgaggaacgt tgcctcctcc
     1441 atcgctgtgt cgccaccgcc gaccacgacg agctcgcggt cgcggaagaa gaacccgtcg
     1501 cacgtggcgc aggcgctcac cccgcgcccc cgcagggtct cctcgccggg gacgccgagc
     1561 catcgcgcgc tcgcgccggt cgccaggatc agcgtctgcg cacgccattc caggtcgccg
     1621 gcccacacgc cgaaagggcg ctcggacagg tccacccgcg tcacggtctg ggccacgatc
     1681 tcggtctcga accgggccgc ctgcttctcg aacgcgtcca tcatctcggg tccgaggatg
     1741 ccgtcgggga accccggata gttctcgacg tccgtggtca gcatcagctg cccgccggcc
     1801 tcgagcccct tgaccagcac ggggcgaagg ttcgcccggg c
//
LOCUS       DATBRK010000239         3809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_901213, whole genome shotgun sequence.
ACCESSION   DATBRK010000239 DATBRK010000000
VERSION     DATBRK010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901213"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VIH70_07240"
     CDS             complement(<1..992)
                     /locus_tag="VIH70_07240"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY8116191.1"
                     /translation="MQKQPKFVFVTGGVASGLGKGITTASLGRLFRSRGLRVALQKLD
                     PYVNVDPGTMNPFEHGEVFVLDDGAETDLDLGHYERFTDENLHRGSNLTTGAVYSAVI
                     AKERRGDYLGKTVQVIPHITDEIKDRIRNHAAAENADLVIVEVGGTVGDIESLPFLEA
                     IRQLRNEVGRDRCAFVHVSLMPFIGPSGELKTKPTQHSVKELRSLGLQPDAIVCRSDR
                     PIGRHLKEKISLLCDVPISGVVSAQDADSIYRVPLILAKEGLDAELAQHLRIDAEPDM
                     SEWQTLVGRIDAAVDPVRVAIVGKYVNLRDAYLSVIEALKHGGFHHGVDVQIDWV"
     gene            complement(1077..1814)
                     /locus_tag="VIH70_07245"
     CDS             complement(1077..1814)
                     /locus_tag="VIH70_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116192.1"
                     /translation="MRWGRLLVENHRGVPIPRVLGIALAIDAVVWTLVVATIQEVGAA
                     GWGALAGLLLVFGAGLIDDLAPPGPRGLRDHLRSVASGRATTGLIKVVVIVGSAAVVV
                     ALQPGRPTPVAVLGVVLLSASSNVWNGLDVVPGRSLKAFVAPALAFVIWGVSSDAPAV
                     VGLLVGAVVVLPFDLRESAMLGDGGSNLLGLAAGIALYDTLPDPWVAVAAVVAVVLNV
                     VAETLSFSRVIERTPPLRWFDGLGRRA"
     gene            complement(1834..2553)
                     /locus_tag="VIH70_07250"
     CDS             complement(1834..2553)
                     /locus_tag="VIH70_07250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8116193.1"
                     /translation="MSRGAHGAGPNARALASRERRVLALVAAHDEAGRIGPCVSALRG
                     LADEVVVVDDGSRDRTASEATSAGANVVRSPRRRGKGGALEGALRRIGPADVWLLADG
                     DLGATAGNLARLLEAVTSGEADLAIAILPPARAGGFGLVKRFSAHLIRGQTGFRASEP
                     LSGQRALTSHAMEAVRPLAGGFGVETAMTIDAVRAGLRVVEIPIGGLEHRPTYRTPRG
                     FLHRGWQGWDIARAVLPRMLR"
     gene            complement(2550..3464)
                     /locus_tag="VIH70_07255"
     CDS             complement(2550..3464)
                     /locus_tag="VIH70_07255"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEY8116194.1"
                     /translation="MISFRYHIVTIVAIFLALAIGLLGGAAFVQPALQQQLQDQTDRL
                     RNDLDDRTQEIGDLRDQLGALNGFADSVMPYLTNGTLSGTPVVVVTQAGVEDEVLTQA
                     QAALASADARILTTVSATDQLVSEDPTAQDQLATIVGEPTAPAEELPRLAAEALAQGL
                     SPSTTVIGGEVLRDLLSAGFLAPIGPGPSQATLEEIGAPGQVVVVLSGGRGEEPTLPP
                     EAFAVPLVERLAGLGVPVAAGESLLTDYPYVSLVRADGIDGTVTIDDLDQSMGGAALV
                     LGLEDLLASGTGGAYGVKDGAEPLPPAP"
     gene            complement(3461..>3809)
                     /locus_tag="VIH70_07260"
     CDS             complement(3461..>3809)
                     /locus_tag="VIH70_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SteA domain-containing protein"
                     /protein_id="HEY8116195.1"
                     /translation="GTSEDAAFLLAHEKGAETIVAVGSHGNLREFLDKGREGMSSTFL
                     VRLRVGEILMDAKGVSRVYSPRIRTRDALLLVVAALVAIAAVVVVSPPLRLYVSQLAE
                     QFRQWLFDLKEFL"
BASE COUNT          577 a         1380 c         1346 g          506 t
ORIGIN      
        1 acccagtcga tctggacgtc gacgccgtgg tggaacccgc cgtgcttgag cgcctcgatc
       61 accgacaggt acgcgtcccg caggttcacg tacttgccga cgatcgccac gcgcaccggg
      121 tcgacggccg cgtcgatccg gccgacgagg gtctgccatt cggacatgtc gggctcggcg
      181 tcgatccgca ggtgctgcgc gagctccgcg tcgaggccct ccttcgccag gatcagcggg
      241 acgcggtaaa tcgagtcggc gtcctgggcc gagacgacgc cgctgatcgg cacgtcgcac
      301 agcaggctga tcttctcctt caggtgtcgc ccgatcggcc ggtccgaacg gcacacgatc
      361 gcgtccggct gcagaccgag cgagcgcagc tccttcaccg agtgctgcgt cggcttggtc
      421 ttcagctcgc cggacggtcc gatgaacggc atcaggctga cgtggacgaa cgcgcaccga
      481 tcgcgtccca cctcgttgcg gagctggcgg atcgcctcca ggaacgggag cgactcgatg
      541 tcgccgaccg tccccccgac ctcgacgatc acgaggtcgg cgttctcggc cgcggcgtga
      601 ttgcggatcc ggtctttgat ctcgtcggtg atgtgcggga tcacctgcac ggtcttgccg
      661 aggtagtcgc cccgacgctc cttggcgatc acggcgctgt agacggcgcc ggtcgtgagg
      721 ttcgatccgc gatggaggtt ctcatccgtg aagcgttcgt agtggccgag gtcgaggtcg
      781 gtctcggcgc cgtcgtcgag cacgaacacc tcgccgtgct cgaacgggtt catcgtcccc
      841 gggtcaacgt tcacgtatgg gtcgagcttc tggagcgcga cccggagccc ccgggagcgg
      901 aagagccggc cgagcgacgc ggtggtgatc cccttgccga ggcccgaggc cacaccaccg
      961 gtcacgaaga cgaatttcgg ctgtttctgc acctcgggat ggcatcgtag cagaggcggg
     1021 tgacgcgccg gcccgtttcc ctcaccaagt gcccggaccg acggaaaacc tcgccatcag
     1081 gcgcgccgtc cgagcccgtc gaaccaccga agcggcgggg tgcgttcgat cacgcggctg
     1141 aaggacaagg tctcggccac gacgttcagg acgacggcga cgacggcggc caccgcgacc
     1201 caggggtccg gcagggtgtc gtagagcgcg atccccgccg ctaaccccag caggttcgac
     1261 ccgccgtcgc ccagcatcgc gctctcgcgc aggtcgaacg ggagcacgac gacggcgccc
     1321 acgagcagtc cgacgaccgc gggcgcgtcc gacgacacgc cccagatcac gaacgcgagc
     1381 gccggcgcga cgaaggcctt gagggatcga ccggggacga cgtccaggcc gttccacacg
     1441 ttcgaggacg ccgagagcag cacgacgccg agcacggcga ccggggtcgg ccggccgggc
     1501 tgcagggcga ccacgaccgc ggccgagccg acgatcacga cgaccttgat cagccccgtc
     1561 gtggcgcggc ccgaggcgac cgaacgcagg tggtcgcgca ggccgcgcgg tcccgggggc
     1621 gcgagatcgt cgatcagacc ggcgccgaac accagcagca gacccgcgag cgcgccccac
     1681 ccggcggcgc cgacctcctg gatcgtggcg acgacgaggg tccacacgac ggcgtcgatc
     1741 gcgagcgcga tcccgagcac gcgggggatc ggcacgccgc ggtggttctc cacgaggagc
     1801 cgcccccacc tcaccggggc gcgtccgagc gggtcatcgc agcatccgag gcaggacggc
     1861 cctcgcgatg tcccacccct gccaaccgcg gtggaggaac ccccgcgggg tccggtacgt
     1921 cgggcgatgc tcgagaccgc cgatcgggat ctcgaccacc cgcagtccgg cgcggaccgc
     1981 gtcgattgtc atcgcggtct cgaccccgaa cccgcccgcg agcggccgta cggcctccat
     2041 cgcgtgcgac gtgagcgcgc gttgcccgga cagcggctcg gaggcgcgga acccggtctg
     2101 cccccggatc aggtgggcgg agaagcgctt cacgagcccg aagcctcccg ctcgcgccgg
     2161 cggcaggatc gcgatcgcga ggtccgcctc gcccgacgtc accgcctcga gcagccgggc
     2221 caggttgccg gccgtggcgc cgaggtcgcc gtccgccagc agccacacgt ccgccggtcc
     2281 gatccggcgc agcgcgccct cgagcgcgcc gcccttgccc cggcggcggg gcgaccgcac
     2341 cacgttcgcg ccggccgacg ttgcctcgga tgcggtccgg tcccgagacc cgtcgtccac
     2401 gacgacgacc tcatcggcga gccctcgcag cgccgagacg cacgggccga tccgcccggc
     2461 ctcgtcgtgg gccgcgacga gcgcgaggac ccgtcgctca cgggaggcga gcgcccgagc
     2521 gttcggtccc gctccgtgag caccccggct cacggtgccg gcgggagcgg ttcggcgccg
     2581 tccttcacgc cgtacgcgcc gccggtcccg gaggcgagca ggtcctcgag acccagcacc
     2641 agcgccgcgc cccccatcga ttggtcgaga tcgtcgatcg tcacggtgcc gtcgatgccg
     2701 tcggcgcgca cgagcgagac gtaggggtag tcggtcagca gcgactcccc cgccgccacg
     2761 gggacgccga gtccggccag ccgttcgacg agcgggacgg cgaacgcctc gggggggagc
     2821 gtcggttcct ccccgcggcc accggagagc acgacgacga cctgaccggg agcgccgatc
     2881 tcctcgaggg tggcctggga ggggccgggt ccgatcggcg ccaggaaccc cgcggacagg
     2941 aggtcgcgaa ggacctcgcc cccgatcacg gtcgtcgacg gcgacaggcc ctgagccagc
     3001 gcctcggccg cgagccgcgg gagctcctcc gccggcgcgg tgggctcccc cacgatcgtc
     3061 gccagctgat cctgggcggt cggatcctcc gacacgagct ggtcggtggc cgacacggtc
     3121 gtcaggatgc gcgcgtcggc gctcgccaac gccgcctggg cctgggtgag cacctcgtcc
     3181 tccacgccgg cctgggtgac gacgacgacc ggtgttccgc tcagcgtccc gttcgtcagg
     3241 tagggcatga ccgagtccgc gaagccgttc agcgcgccga gttggtcgcg gaggtcgccg
     3301 atctcctgag tgcgatcatc gagatcgttc cggaggcgat cggtctgatc ctggagctgt
     3361 tgctgcagcg cgggttgcac gaacgccgcg ccgccgagga gtccgatcgc gagcgcgagg
     3421 aagatcgcga cgatcgtcac gatgtggtac cggaacgaga tcacaggaat tctttcaggt
     3481 cgaacagcca ctggcggaac tgttcggcga gctgcgagac gtacagccgc agcggcggcg
     3541 agaccacgac cacggccgcg atcgcgacga gcgcggcgac caccagcagg agcgcgtcgc
     3601 gtgttcggat ccgcggcgag tagacgcgcg agactccctt cgcgtccatc aggatctcgc
     3661 ccacgcggag ccggaccagg aaggtgctcg acatcccttc ccggcccttg tcgaggaact
     3721 cgcggaggtt gccgtggctg ccgaccgcca cgatcgtctc ggcgcccttc tcgtgggcga
     3781 gcaggaacgc ggcgtcctcg ctggtgccg
//
LOCUS       DATBRK010000240         7703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_901429, whole genome shotgun sequence.
ACCESSION   DATBRK010000240 DATBRK010000000
VERSION     DATBRK010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901429"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..245
                     /locus_tag="VIH70_07265"
     CDS             75..245
                     /locus_tag="VIH70_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116196.1"
                     /translation="MSEGTEETKKRGFFGRLMKIAFIAAIVAAVVAVFKRRRGKDLDE
                     DEWQELPPPSGG"
     gene            364..1179
                     /locus_tag="VIH70_07270"
     CDS             364..1179
                     /locus_tag="VIH70_07270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8116197.1"
                     /translation="MERDPWELAFEAARAAGVELRPLAELEDADRILRVMDATWPGPE
                     PFPREVLRALADSGNVPYGALDGDETIGYVLGWVGVDPEDGLHTHSHMLAALPGRRHR
                     GVGYALKLAQRAQALDQGIAEVRWTFDPLVARNGYFNLHKLGAVADRFDRNHYGAMVD
                     EVNAGERSDRFTVRWDLGREPGPRDVGAHEATLEDDGGWPGDVHAPSGRGALVAVPSD
                     YARLRTDDAACASAWRDASADAIEACLTAGMIAAGFDRVASSYAFAPAEDVPR"
     gene            1176..2288
                     /gene="menC"
                     /locus_tag="VIH70_07275"
     CDS             1176..2288
                     /gene="menC"
                     /locus_tag="VIH70_07275"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEY8116198.1"
                     /translation="MTRIRSIELRLVAIPLVRPFRTSLGESTEKVCILARVETDDAVG
                     WGECVSDVEPTFSEEWNGGAWLMIRDFLAPALFAAGDVEAELVPSVLSFVRGHPMAKA
                     TLVNAVLDAELRARGRSLASYLGAARDRVECGVSVGIAPSAEVLLEQVAGYLAEGYRR
                     IKLKIEPGADVERVRAIREANPEILLSVDANAAYRLADVDVFRELDAFGLLMIEQPLH
                     HEDLWQHSMLQREIRTDICLDESIRSAADARAAIELGACRIVNIKQGRVGGLLEARRV
                     HDVASELGVPVWCGGMLETGIGRSTNLALAALPNFRLPGDTSATSRYFAEDLTEPLVL
                     APDGTLPVPDRPGIGVEPDPRRLEAATRRTERLEKE"
     gene            2537..3172
                     /locus_tag="VIH70_07280"
     CDS             2537..3172
                     /locus_tag="VIH70_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116199.1"
                     /translation="MRKRVIRAGEIEIEDSEGRVRARFGVSNDDLPFMSFYGADDKLR
                     ARFGVQPDGSAGLAIADDDGKVRATFGLFSDGSASMAMVDRNGKVRAKFGLGTEGSPT
                     LALRNKDGQLGFVYSLAQQGSPTISLQDEDGKVRARIGLAAEGSPILRLLNKEGELRA
                     IMGLTADGTPVMQFLDEKGEPEWTAPSSFSPSHDVPNQTPKPSRPRARERG"
     gene            3178..3687
                     /locus_tag="VIH70_07285"
     CDS             3178..3687
                     /locus_tag="VIH70_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116200.1"
                     /translation="MSGWWAAAFIVQWILVAVLAIVVVALARQIGVLQLRLAPLGALE
                     VDDEGPPLGEAPQARHVRTPDGSARVVGGSGRGRLVAFVSDTCPICEQVAPSLPAAAR
                     ASGLELQLVSDPDLETAYSVPGVPFVVVLDEVGVVRSKGTVNSLEQVEGLVDAARRRT
                     MQAEELRAS"
     gene            3687..4211
                     /locus_tag="VIH70_07290"
     CDS             3687..4211
                     /locus_tag="VIH70_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116201.1"
                     /translation="MGAVERAGTELARRTSRRSFLGRLGRSVVAIAGGSMVAVALAPD
                     RAEAHHICGHLYTTGSCPHPFSPLSRTDAYGYPVHPRLGYPVDDEGAIYLSGRQTRRK
                     VCQAVVPERFPFVRNPRYGGGWSRCCGGRVRHIQDCCSTADTRINGDGAVRGYCPRAR
                     KVFCITYRELNVRC"
     gene            4295..5422
                     /locus_tag="VIH70_07295"
     CDS             4295..5422
                     /locus_tag="VIH70_07295"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY8116202.1"
                     /translation="METITPVVHGGSRSRWGISLALHAIGAAAAAAIAGSLLAGAGAL
                     LGAPWGVPGAVLVASAAALYVARELGAPVPVPQLRRQVPDWWRTFFPPHVAAFLYGLG
                     LGPGFLTYLGHGTVVVVSVAAFASGRPLVGAAVLAPFGLARGLGPVLAFGVRSPGDAA
                     ALVERLDRFASRGRLRVANALALSMVVVATILEGRTIDGPSEVGALAAAALALTFGAA
                     AVAKLARRAAWRRTLGSYGLGPGVSRLVGFGVPAVELGIAALVLAGLGSSAGLLSIVA
                     LVAFSGAIVLGRVRAGRRLECGCLGGSATRDYRLLLARNLALGGVALVAWRAGEDASL
                     VRSLGEPAGADLLPLALVVLGLALAAWVGVAFGGVARRSGG"
     gene            5419..6090
                     /locus_tag="VIH70_07300"
     CDS             5419..6090
                     /locus_tag="VIH70_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116203.1"
                     /translation="MRRLALGIGAMAVVTLTAFALPVLANHTDQVDRHDTGGRLDLAE
                     VVFDHVAAPTWRMSTFPTWTVRSIWDLGTFVVQLDTRGDAAADFVAVVRSDGRRLRAD
                     LFRLRRDGREIEMTTLPTAKAGSRAVTVTVPLRRLSIGPNRTSYFWSVLSSFTGGLLC
                     RRTCLDAIPDEGMIEQPLPGVTPSPTTTTTPTPTTTTTTTTTTTTTTIPPSTTTTTTT
                     VAVSG"
     gene            complement(6301..7374)
                     /locus_tag="VIH70_07305"
     CDS             complement(6301..7374)
                     /locus_tag="VIH70_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEY8116204.1"
                     /translation="MLKRTNVPLVVASLLALLAPGTATAAQGGDYTVTNLVSDQPGVA
                     AHLDPNLVNAWGLAAGPMTPWWVADADADVSTLYQADGTAVPLVVDVPGGPTGLVFNG
                     SGGFVVTDGTTSGSSLFLFATEAGTIRGWSPAVPPPPLSTQTFVGYDGSDEDANYKGL
                     AIASTDAGTVLYGADFHNAEVEVFDDTFQEVTPEGAFEDPKLPEGFAPFGIQTIGDAV
                     FVTYAKQDADAEDEVAGAGLGFVDEFSTNGEFIQRVASKRHLNAPWGLAMAPESFGAF
                     GGDLLVGNFGDGRINAFEPLPNGKFVFREQLESEGALLSIDGLWSLQFGNDSGAGPSQ
                     TLFFTAGPEDETHGLFGSIDPAA"
BASE COUNT         1012 a         2647 c         2871 g         1173 t
ORIGIN      
        1 agagcgcatc cctgataagg atgaggtcgt tggttcaagt ccaaccaggc ccacccggcg
       61 aggaggaggc ggcgatgtcc gaaggaactg aggaaacgaa gaagcgcggc ttcttcggca
      121 ggctgatgaa gatcgctttc atcgcggcga tcgtggcggc cgtcgtagcc gtcttcaaac
      181 gccggcgggg caaggatctg gacgaggacg agtggcagga gctgccgccg ccctcgggcg
      241 ggtagccgag ccggacacgt tccgccgagg ggcccgcgct cgtcgcgggc ccctcgcgtt
      301 gtggccgcgg atgccctgtt gccggtcccg gcatgggtcc cgggatggct agactccggc
      361 gccgtggagc gcgacccctg ggagctggcg ttcgaggcgg ctcgcgccgc cggcgtcgag
      421 ctccgaccgc tcgccgagct cgaggacgcg gatcgcatcc tgcgggtgat ggacgccacg
      481 tggccgggcc ccgagccctt cccgcgcgag gtgctccgcg cgctggccga cagcgggaac
      541 gtgccgtacg gcgcgctcga tggcgacgag acgatcgggt acgtcctggg atgggtcggt
      601 gtggatcccg aggacgggct gcacacccac tcgcacatgc tcgccgccct tccgggtcgg
      661 cgtcatcgag gggtcgggta cgcgctcaag ctcgcgcagc gggcgcaggc gctcgaccag
      721 gggatcgccg aggtccggtg gaccttcgat cccctcgtcg cccgtaacgg gtacttcaac
      781 ctgcacaagc tcggcgcggt cgccgatcgc ttcgaccgca accactacgg ggcgatggtc
      841 gacgaggtca acgccggcga gcggagcgac cggttcaccg tccgatggga cctcggacgc
      901 gaacccggcc cgagggacgt gggcgcgcac gaggccacgc tcgaagacga cggcggatgg
      961 cctggcgacg tccatgcgcc gagcgggcgc ggggcgctcg tcgcggtccc gagcgactac
     1021 gcgcgcctgc ggaccgacga tgccgcgtgc gcgtcggcgt ggcgcgacgc gtcggccgat
     1081 gcgatcgagg cctgtctgac ggcggggatg atcgcggcgg ggttcgaccg cgtcgcctcg
     1141 agctacgcgt tcgcgccggc cgaggacgtg ccccggtgac gcggatccgt tcgatcgagc
     1201 ttcgcctggt ggcgatcccg ctggtccggc cgttccggac cagcctcggc gagtccaccg
     1261 agaaggtctg catcctcgcg cgcgtggaga ccgacgacgc ggtcgggtgg ggggagtgcg
     1321 tcagcgacgt cgagcccacc ttctccgagg agtggaacgg cggggcctgg ctgatgatcc
     1381 gcgacttcct cgcgcccgcg ttgttcgccg cgggggacgt cgaggccgag ctggtcccct
     1441 cggtcctctc gttcgtccgc gggcacccga tggccaaggc cacgctcgtg aacgcggtgc
     1501 tcgacgccga gctccgagcg cggggccgtt cgctcgcgtc ctacctggga gcggcccgcg
     1561 accgggtcga gtgcggcgtc tcggttggga tcgcgccctc ggccgaggtg ttgctcgagc
     1621 aggtcgcggg ctacctcgcc gagggctacc gccgcatcaa gctcaagatc gagccgggcg
     1681 ccgacgtcga acgcgtccgc gcgatccggg aggcgaaccc cgagatcctg ctctcggtgg
     1741 acgcgaacgc cgcctaccgg ctcgcggacg tcgacgtgtt ccgggagctc gacgcgttcg
     1801 ggctgttgat gatcgagcag ccgttgcacc acgaggacct gtggcagcac tcgatgctcc
     1861 agcgggagat ccggaccgac atctgtctgg acgagtcgat ccgctcggcc gcggacgccc
     1921 gggcggcgat cgagctcggg gcgtgccgga tcgtcaacat caagcagggg cgcgtcggcg
     1981 ggctgctcga ggccaggcgc gtgcacgacg tcgcgtcgga gctcggcgtg cccgtgtggt
     2041 gcggggggat gctcgagacc gggatcgggc gttcgacgaa cctcgcgctg gccgcgctcc
     2101 cgaacttccg gctgcccggc gataccagcg cgacgtcgcg atacttcgcg gaggacctga
     2161 ccgagccgct cgtcctggcg cccgacggca cgctgccggt ccccgaccga cccgggatcg
     2221 gcgtcgaacc cgacccccgg cgactcgagg ccgccacccg gcggacggag cggttggaga
     2281 aagagtgaca aaagcgttcg agccgggttg accgtctcga cctgcgtccg tacagtccgc
     2341 tgtgagagac cgctgtgacc ttcgggtcgc ggcggtttct cacgtctcgg gcctcgagtc
     2401 cccgcacacc tcccatcgag gacccgagtg cggcgtcggt gacgccatcg cgggccgctt
     2461 cggcgacgca aggcgcacat cgcggcgccc gctcgtccta gactcacgtc gacgtccggt
     2521 cgaggagagc tgcgggatgc gcaaacgggt catcagggcg ggcgagatcg agatcgagga
     2581 ctcggagggg cgggttcgcg cccggttcgg cgtctcgaac gacgacctcc cgttcatgtc
     2641 gttctacggc gcggacgaca agctgcgcgc tcgtttcggg gtgcagcccg acggctcggc
     2701 cggcctggcg atcgccgacg acgacggcaa ggtccgcgcc acgttcgggc tgttctccga
     2761 cgggtcggcg agcatggcga tggtggatcg caacggcaag gtgcgtgcga agttcggtct
     2821 gggcaccgag ggctcgccca cgcttgcgct gcgcaacaag gacggccagc tcggcttcgt
     2881 gtactcgctc gcgcagcagg gttcgccgac gatctcgctg caggacgagg acggcaaggt
     2941 gcgcgcccgg atcgggctgg cggcggaggg ttcgccgatc ctgcggctgc tgaacaagga
     3001 gggagagctc cgcgcgatca tggggctgac ggccgacggg acgcccgtga tgcagttcct
     3061 cgacgagaag ggcgagcccg agtggaccgc accgagctcg ttctcgccgt cccatgacgt
     3121 tcccaaccag acgccgaaac cctcccggcc cagggctcgg gagcggggct gacccgcgtg
     3181 tcggggtggt gggccgcagc cttcatcgtc cagtggatcc tggtcgccgt gctggcgatc
     3241 gtcgtggtcg cgctcgcgcg ccagatcggc gtgctgcagc tccgcctggc cccgctcggg
     3301 gcgctcgagg tcgacgacga gggcccaccg ctcggtgagg cgccccaggc ccgacatgtc
     3361 cgaacgcccg acggctccgc gagggtggtc ggcgggtcgg ggcggggccg gctggtggcg
     3421 ttcgtctcgg acacctgccc gatctgcgag caggtggccc cgtcgctgcc cgcggccgct
     3481 cgtgcgagcg ggttggagct ccagctcgtc tccgatccgg atctcgagac cgcgtacagc
     3541 gtcccgggcg tgccgttcgt ggtggtgctc gacgaggtcg gcgtcgtccg gtccaagggg
     3601 accgtgaact cgctcgagca ggtcgagggt ctggtcgacg ccgcgcggcg ccgaacgatg
     3661 caggccgagg agctccgggc cagttgatgg gggccgtcga gcgagccggg accgagctcg
     3721 cgcgccggac gagccgacgg tcgttcctcg gcaggctcgg gcggtcggtc gtcgcgatcg
     3781 ccggcggctc gatggtggcg gtcgcgctcg cgcccgaccg cgcggaagcc caccacatct
     3841 gcgggcacct gtacacgacc ggctcgtgcc cgcacccgtt ctcgccgctc tcgaggacgg
     3901 acgcctacgg gtacccggtg catccgcggc tgggctaccc ggtcgacgac gagggcgcga
     3961 tctatctgtc gggacggcag acgcggcgga aggtctgcca ggccgtggtg ccggagcggt
     4021 tcccgttcgt gcggaacccg cggtacggcg gcggctggag ccggtgctgc ggcggacgcg
     4081 tgcggcatat ccaggactgc tgctccaccg ccgacactcg gatcaacggc gacggcgcgg
     4141 tgcggggcta ctgcccccga gcccgcaagg tcttctgcat cacgtaccgg gagctcaacg
     4201 tccggtgctg agcgagccgg tcggtctcgc gctcgtcgtc gcgtcggtcg tcgcggggat
     4261 ctcgagcctg ttcggcccct gaggcctgtc gatggtggag accatcaccc ccgtggtcca
     4321 cgggggaagc cggagccgat gggggatctc gctggccctg cacgcgatcg gcgccgccgc
     4381 ggccgccgcg atcgccgggt cgctcctggc gggggctggt gcgctgctcg gcgcgccgtg
     4441 gggggttccg ggcgccgtgc tcgtcgcgtc ggcggccgcg ctctacgtcg cgcgcgagct
     4501 cggggcgccg gtgccggtgc cgcagctgcg gcggcaggtg ccggactggt ggcgcacgtt
     4561 cttcccgccg cacgtcgcgg cgttcttgta cgggctcggc ctcgggcccg ggttcctcac
     4621 ctacctgggg cacgggacgg tcgtcgtggt gtccgtcgcc gcgttcgcga gcggccggcc
     4681 gctcgtgggg gcggcggtgc tcgcgccgtt cggtctggcc cgcgggctgg gtccggtcct
     4741 cgcgttcggc gtgcgttcgc cgggcgacgc ggcggccctc gtcgagcgcc tcgaccgttt
     4801 cgcgtcgagg gggcgcttgc gggtcgcgaa cgccctcgcg ctctcgatgg tggtcgtggc
     4861 gacgatcctc gagggccgga cgatcgacgg tccgtcggag gtcggggcgc tcgcggccgc
     4921 ggcgctggcg ctgacgttcg gggcggcggc cgtcgccaag ctcgcgcggc gagccgcgtg
     4981 gaggcggacg ctcgggtcgt acggtctcgg gcccggggtg tcgcggctcg tggggttcgg
     5041 ggttcccgcc gtcgagctcg gcatcgcggc gctcgtgctc gcggggctcg ggtcgtcggc
     5101 gggactccta tcgatcgtcg cgctggtggc cttctccggg gcgatcgtgc tgggacgcgt
     5161 tcgggcgggc cggcgtctgg agtgcgggtg cctcggcggg tcggcgacgc gcgactaccg
     5221 gctgctgctc gcccgtaacc tcgcgctcgg cggggtggcg ctcgtcgcgt ggagggcggg
     5281 ggaggacgcg tcgctcgtgc gctcgctcgg cgagcccgcc ggcgccgacc tgctccccct
     5341 cgcgctcgtc gttctcggtc tcgcgctcgc ggcatgggtc ggggtcgcgt tcgggggcgt
     5401 cgcccggagg agcgggggtt gaggcgtctc gcgctgggga tcggggcgat ggcggtggtg
     5461 acgctgacgg cgttcgcgct tccggtcctc gcgaaccaca ccgaccaggt cgaccggcac
     5521 gacacgggcg gaaggctgga cctggcggag gtggtcttcg atcacgtggc cgcacccacc
     5581 tggcggatgt cgacgttccc cacgtggacc gtgcgctcga tctgggatct cgggaccttc
     5641 gtcgtgcagc tcgacacgag gggcgacgcc gccgccgact tcgtcgccgt cgttcgctcc
     5701 gacggacggc ggctgcgggc ggacctgttc cgcctccggc gggacgggcg cgagatcgag
     5761 atgacgacgc tcccgaccgc caaggccggc tcgagggccg tgacggtgac ggtcccgctg
     5821 cgccggctct cgatcgggcc gaaccgcacc tcgtacttct ggtcggtgct ctcgagcttc
     5881 acgggcgggc tcctctgcag gcgaacgtgt ctcgacgcca tccccgacga ggggatgatc
     5941 gaacaaccac tgccgggggt cacgccgtcg ccgaccacga cgacgacccc caccccaacc
     6001 acgaccacga ccacgaccac gaccacgacg acgaccacga tcccgccctc aactacgacc
     6061 acgacgacga ccgtcgcggt gtccggctga agccggcgag ccaggatccg catcgaatga
     6121 tgtgagtctt cgcggacgta gcccagttgg cagacgtagc ccagtgggta gaggtgttgc
     6181 ggcgtgtcat cggcgatcac gaacgctcat gcaactcgtg gacctggagc acgtcgccga
     6241 ccagcgagct gtggatcgct cgctggtcga cgacgtcgct cagccgaccc gatcagtgct
     6301 tcaggccgcc ggatcgatcg acccgaacag gccgtgggtc tcgtcctcgg gtcccgcggt
     6361 gaagaacagc gtctgcgagg gcccggcgcc cgagtcgttg ccgaactgca gggaccacag
     6421 gccgtcgatc gacaggagcg ctccctccga ctcgagctgc tcgcggaaga cgaacttccc
     6481 gttcggcaac ggctcgaacg cgttgatccg tccgtcgccg aagttgccga cgagcagatc
     6541 gccgccgaac gctccgaagc tctcgggagc catcgcgagg ccccacggcg cgttcaggtg
     6601 gcgcttcgag gcgacgcgct ggatgaactc accgttcgtc gagaactcgt cgacgaaccc
     6661 gagcccggca cccgcgacct cgtcctcggc gtcggcatcc tgcttcgcgt acgtcacgaa
     6721 cacggcatcg ccgatcgtct ggatcccgaa cggcgcgaac ccctcgggca gcttcgggtc
     6781 ctcgaatgcc ccctcgggcg tcacctcctg gaaggtgtcg tcgaacacct cgacctccgc
     6841 gttgtggaag tcggcgccgt acagcaccgt ccctgcgtcg gtcgaggcga tcgcgaggcc
     6901 tttgtagttt gcgtcctcgt ccgatccgtc gtagccgacg aatgtctggg tcgacagcgg
     6961 cggcggcggg accgccggac tccatcctct gatcgttccg gcctcggtcg cgaacaggaa
     7021 cagtgacgac cccgaggtcg tcccgtcggt gacgacgaag ccaccggacc cgttgaagac
     7081 gagcccggtc ggtccgccgg ggacatcgac gacgaggggt acggccgtcc cgtcggcctg
     7141 gtagagcgtg gagacgtccg cgtcggcgtc ggcgacccac cagggcgtca tcggccccgc
     7201 cgcgagtccc caggcgttca cgaggttcgg atccaggtgc gcggccacgc cgggctggtc
     7261 ggagaccagg ttcgtaaccg tgtagtcacc gccttgcgcg gcggtcgcgg tgccgggagc
     7321 gaggagcgcg agcaacgacg ctacgaccag tggcacgttc gttctcttca gcatctgtcc
     7381 tcccgcaggt cgtgtgcgag ctcagcgcgt gccaactcca cctcccttct gcggctcgac
     7441 ggcaggcagg gcacgccgag gtactacgga cgggaacgcg aaggggtttg gccgaggaac
     7501 cggccgtccg caccggaggc gtcaaccagt gcctcgaccc aacttggtcg ccgaaccgtt
     7561 ctgcctgctc atccgtagac ccacggagta ccgtgaatct cgtgcgaacg gatgagccgg
     7621 gcagcgacct cgctctgatg gcggatttcg cggcccgccg ggagcgtgcg gccggcgtgc
     7681 tctacgaccg gttcgcgtcc agg
//
LOCUS       DATBRK010000241         5186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2202913, whole genome shotgun sequence.
ACCESSION   DATBRK010000241 DATBRK010000000
VERSION     DATBRK010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202913"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VIH70_07310"
     CDS             complement(<1..471)
                     /locus_tag="VIH70_07310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8116205.1"
                     /translation="MTTYPMSEADREIQERARRFMDEELIPLEIETELSGGKLSEDVR
                     EKHHHMAVDLGLWAMNMPKELGGGGLSMFQQVLVSEQIGRVTNGLGWCIHTPPEWAPA
                     VVSREQLERWILPTIRGERQECYAITEEVAGSDVDAIQATARRDGDEYVLNGRKW"
     gene            complement(482..1537)
                     /locus_tag="VIH70_07315"
     CDS             complement(482..1537)
                     /locus_tag="VIH70_07315"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEY8116206.1"
                     /translation="MERPDTLVWDAYARALPSMLDAVGRTPLVRLSRVGREGLSLLAK
                     IEWYGPTGSTKDRIYRHMIERAEERGDLTPGMTIVESTTGNAGIACSAVAAIKGYDCV
                     IVMPRGMSPERKRMIEAYGAELVLTPGAGTDIDLAIAKMREIVATDPERYFVPGEFEN
                     PDNPDAQAISGEEIWQQSGGQVDAVVAAQGTGGWITGVARALKAYDRAIRAYAVEPAE
                     SPLISEERWGTHGVPGIGDGIVPHNLDLSLMDGIVTASTEEALEMAHRLAREEGILAG
                     PSGGINVASALKVAEKHPEFATIVTAVPDTGQRYLSGELFGERPDEPASPERDHSVDP
                     DSRRQIEDNRARLEFIS"
     gene            complement(1595..2041)
                     /locus_tag="VIH70_07320"
     CDS             complement(1595..2041)
                     /locus_tag="VIH70_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY8116207.1"
                     /translation="MFAVAAVALVAGGVVGYIALSGPSIVIPDDPCAERPPLRYVDGV
                     RLQPIAMRAFQKAQREAGVPIPVVQSYRSCAAQRRACRSICGDPEGCPDRCAPPGLSW
                     HQLGAAIDVTQEGVDDPAIVSALEDNGWCQSLPGSDPGHFSFDGCH"
     gene            2158..3360
                     /locus_tag="VIH70_07325"
     CDS             2158..3360
                     /locus_tag="VIH70_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEY8116208.1"
                     /translation="MAGPTYWRAAEPKPAYDVVIVGGGGHGLSTAYYLAKNHGITNIC
                     VVEKGWLAGGNMARNTTVIRSNYLWDESSAIYEHSLKLWEGLEEDLGSDLQFSQHGVL
                     NLAHSLGDVRSSMRRLHANRLSGIDAEWLEPEDVKAFCPIINTTPEVRYPVLGGTLQR
                     RGGTARHDKVAWAYATRASELGVDVVEHCEVTGFLRGAGGGVEGVRTTRGDIAAGKVG
                     LVAAGHTSVLAEMLGLRLPVQSHPLQALVSVLLEPVLGCVVMSNAVHVYVSQADKGEL
                     VMGAGVDPYNGYAQRGSFHVIEHQLAAAVELFPVFAHAKLLRTWGGIVDVCPDASPIL
                     SRTPIENLYLNCGWGTGGFKATPGSGWVFSDLIARGEPGPLARPYALERFTTGALIDE
                     HGAAGVAH"
     gene            3360..3704
                     /locus_tag="VIH70_07330"
     CDS             3360..3704
                     /locus_tag="VIH70_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY8116209.1"
                     /translation="MQARTVRRIALELPDAEERETWETATFRVRNKIFVMFAEHERDL
                     WIKSTHDEQEALTAMDPETFFVPPYVGPSGWIGVRIRSVDPDELRELVTEAWRLTAPK
                     RLVRAFDEAEAV"
     gene            3705..3974
                     /locus_tag="VIH70_07335"
     CDS             3705..3974
                     /locus_tag="VIH70_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY8116210.1"
                     /translation="MLLIPCPWCGPREEMEFRYGGQAGIAYPSDPDALSDEAWADFLF
                     MRDNPKGPFTERWYHVHGCRRWFDAVRDTATYAFQRSTPPGGGDT"
     gene            3971..>5186
                     /locus_tag="VIH70_07340"
     CDS             3971..>5186
                     /locus_tag="VIH70_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8116211.1"
                     /translation="MTARRTPSGGSGIDRAKPISLTFDGEEVSAFVGDTIASAILANG
                     IEAPFRSPILGRPRGVFSAGAEEPNAFVETSEPWFDPIVAATMVDVVDGMVVGSRPGV
                     GRLRDDAPRGRPVEHRNAHVELLVVGSGHDGFYPARDAAARGKRVMLVEQHRTIERGA
                     GGDFRAEGVTYLTRATALGLYDDGYVTVLEHADELDRIWHVRGERVILATGAFERSIA
                     FADNDRPGVMLSHAASLYLEHGLLVGSRTVVFTARAGAYGAASNLVGLGGEVRAIADL
                     SGTDPDPRLARDAEVLRGWVVSGTDGDRRLEAVHLRGPDGERRTIECDSLLVSGGWDP
                     NLALWRSIGGGVRFDEGVSAFVPTEGPPWLSVVGSAAGEVVNGVPVWFVPAEDYSRHF
                     VDFQRDQTIADIA"
BASE COUNT          747 a         1838 c         1820 g          781 t
ORIGIN      
        1 ccacttcctg ccgttcagca cgtactcgtc gccgtcccgc cgcgccgtgg cctggatcgc
       61 gtcgacgtcg gagccggcga cctcctccgt gatcgcgtag cactcctggc gctcgccgcg
      121 gatcgtcggc aggatccacc gctcgagctg ctcgcggctg acgacggcgg gggcccactc
      181 gggcggcgtg tggatgcacc acccgagccc gttggtcacc cgccctatct gctcggagac
      241 cagcacctgc tggaacatcg acaggccgcc gccgccgagc tccttcggca tgttcatcgc
      301 ccacagcccg agatcgaccg ccatgtggtg atgcttctcg cggacgtcct ccgagagctt
      361 ccccccgctc agctccgtct cgatctcgag cgggatgagc tcctcgtcca tgaaccggcg
      421 ggcgcgttcc tggatctcgc gatcggcttc ggacatcggg taggtcgtca cggccgcgac
      481 tctacgagat gaactccaac cgggcgcgat tgtcctcgat ctgccggcgc gagtccgggt
      541 cgaccgagtg atcgcgttct ggcgaggcgg gctcgtccgg acgctcaccg aacagctccc
      601 ccgagaggta gcgctgcccc gtgtcgggca ccgccgtgac gatcgtcgcg aactcaggat
      661 gcttctccgc aaccttcagc gcggacgcga cgttgatgcc acccgacggt cccgccagga
      721 tgccctcctc gcgggcgagc cggtgcgcca tctcgagcgc ctcctcggtg gaggcggtca
      781 cgatcccatc catgagcgag aggtcgaggt tgtgggggac gatcccgtcg ccgatccccg
      841 ggacgccgtg ggtaccccac cgttcttcgg agatcagcgg gctctcggcc ggctcgaccg
      901 cgtaggcgcg gatcgcccgg tcgtacgcct tcaacgcccg agcgaccccc gtgatccacc
      961 ccccggtccc ctgcgccgcg accaccgcgt cgacctggcc gccgctctgc tgccagatct
     1021 cctcccccga gatcgcctgc gcatccgggt tgtcggggtt ctcgaactcc cctggcacga
     1081 agtagcgctc gggatcggtg gcgacgatct cgcgcatctt cgcgatcgcg aggtcgatgt
     1141 cggtgcccgc gccgggcgtc aggacgagct cggcgccgta ggcctcgatc attcgcttgc
     1201 gctccgggct catgccccgc ggcatcacga tcacgcagtc gtagcccttg atcgcggcga
     1261 cggccgagca cgcgatcccc gcgttgccgg tcgtcgactc gacgatcgtc atcccgggcg
     1321 tcaggtcgcc gcgctcctcc gcccgttcga tcatgtggcg gtagatccgg tccttcgtcg
     1381 agccggtcgg gccgtaccac tcgatcttgg cgagcaggct gagaccctct cgccccacgc
     1441 gcgagagccg aacgagcggc gtccgcccga ccgcgtcgag catcgacggg agcgctcggg
     1501 cgtaggcgtc ccagacgagc gtgtcggggc gctccatgcc tcagtttgac tcgtcgttca
     1561 atccgagcgc aagccggccg ccggtcccgc catctcagtg gcatccgtcg aacgagaagt
     1621 gaccggggtc gctgccgggg agggactggc accacccatt gtcctcgagc gccgagacga
     1681 tcgccggatc gtccacgccc tcctgcgtga cgtcgatcgc ggcgccgagc tggtgccagg
     1741 acagcccggg cggggcgcag cggtccggac atccctcggg gtccccgcag atcgaacggc
     1801 atgcccgccg ttgcgccgcg cacgaccggt agctctgcac gaccgggatc ggaacgccgg
     1861 cctcccgctg cgccttctgg aacgcgcgca tcgcgatcgg ttgcaggcgg acaccatcga
     1921 cgtatcgcag gggcggacgc tcggcgcacg gatcgtccgg gatcacgatc gacgggcccg
     1981 agagggcgat gtagccgaca acacccccgg ccaccagcgc caccgccgcc acggcgaaca
     2041 ccaggaagcg gcggcgactc ctcttcgagc gggcggcgtg gtgacgtcgg acgaccgtcg
     2101 ggcgatgcta ccgccccgtc gaaagcagcc gcccgtgccc ttgtgcagaa tctccccatg
     2161 gccggaccca cctactggcg agccgcggag ccgaaacccg cctacgacgt cgtgatcgtc
     2221 ggcggcgggg ggcacggcct gtccaccgcg tactacctgg caaagaacca cgggatcacg
     2281 aacatctgcg tggtcgagaa gggttggctc gccggcggca acatggcccg caacacgacc
     2341 gtgatccgct cgaactacct gtgggacgag agctcggcca tctacgagca ctcgctcaag
     2401 ctctgggagg ggctcgagga ggacctgggc tccgacctcc agttcagcca gcacggcgtg
     2461 ctgaacctcg cgcactcgct cggcgacgtc cggtcctcga tgcggcggct gcacgcgaac
     2521 cgattgagcg gcatcgacgc cgagtggctg gagcccgagg acgtgaaggc gttctgcccg
     2581 atcatcaaca ccacgcctga ggtccggtat cccgtactcg gagggacgct ccagcgccgc
     2641 ggcggcaccg cccggcacga caaggtcgcg tgggcgtacg ccacgcgcgc gagcgagctc
     2701 ggcgtcgacg tcgtcgagca ctgcgaggtg acggggttcc tccgcggggc cggcgggggc
     2761 gtcgagggcg ttcggaccac gcggggggac atcgcggccg gcaaggtggg gctcgtcgcc
     2821 gccggccaca cgagcgtcct cgccgagatg ctcggcctca ggctcccggt gcagtcgcac
     2881 ccgctgcagg cgctcgtctc ggtcttgctc gagccggtgc tcgggtgcgt cgtgatgtcg
     2941 aacgcggtgc acgtctacgt gtcgcaggcc gacaagggcg agctcgtgat gggcgcaggg
     3001 gtcgacccgt acaacggcta cgcgcagcgg ggctcgttcc acgtgatcga gcaccagctc
     3061 gccgccgcgg tcgagctgtt cccggtcttc gcgcacgcga agctgctccg gacctggggc
     3121 gggatcgtcg acgtgtgccc ggacgcctca ccgatcctgt cgaggacccc gatcgagaac
     3181 ctgtacctga actgcggctg gggcacgggc gggttcaagg cgacgccggg ctccgggtgg
     3241 gtgttctcgg acctgatcgc tcggggcgag cccggcccac ttgcccggcc gtacgcactc
     3301 gagcggttca cgaccggcgc gctgatcgac gagcacgggg ccgccggggt cgcccactga
     3361 tgcaggcgcg gacggtgcgg aggatcgccc tcgagctccc ggacgccgag gagcgggaga
     3421 cctgggagac cgcgacgttc cgcgtccgga acaagatctt cgtgatgttc gccgagcacg
     3481 agcgcgacct gtggatcaag tcgacgcacg acgagcaaga ggcgctcacc gcgatggatc
     3541 ccgagacgtt cttcgtcccg ccgtacgtcg ggccgagcgg atggatcggc gtgcggatcc
     3601 gctcggtcga ccccgacgag ctgcgcgagc tcgtcaccga ggcgtggcgg ctcacggcgc
     3661 cgaagcgcct cgtgcgagcg ttcgacgagg ccgaggcggt ctgagtgctg ctcatccctt
     3721 gcccctggtg cggtccccgc gaggagatgg agttccgcta cggcggccag gccggcatcg
     3781 cgtatccgtc ggatccggac gccctgagcg acgaggcctg ggccgacttc ctgttcatgc
     3841 gcgacaaccc caagggcccg ttcaccgagc gctggtacca cgtccacggg tgccggaggt
     3901 ggttcgacgc cgtccgggac acggcgacct acgcgttcca gcggtcgacc cctccgggag
     3961 ggggcgacac gtgaccgccc gacggacgcc gtcgggtggg tccgggatcg accgagccaa
     4021 gccgatctcc ctcacgttcg acggcgaaga ggtctcggcg ttcgtgggcg atacgatcgc
     4081 gagcgcgatc ctcgcgaacg ggatcgaggc accattccgg agcccgatcc tcggtcgccc
     4141 tcgcggcgtc ttctcggcgg gcgccgagga gccgaacgcg ttcgtcgaga cctcggagcc
     4201 gtggttcgac ccgatcgtcg ccgcgaccat ggtggacgtc gtcgacggca tggtggtcgg
     4261 aagccgtccg ggcgtcggac gcctccgcga cgatgcaccg cgcggccgcc cggtcgagca
     4321 ccggaacgcg cacgtcgagc tgctcgtcgt gggaagcggc cacgacgggt tctaccctgc
     4381 ccgcgacgcc gcagcgcgcg ggaaacgggt gatgctcgtc gaacagcatc ggacgatcga
     4441 acggggcgcg ggcggcgact tccgggccga gggcgtgacg tacctcacgc gggcgacggc
     4501 gctcggcctg tacgacgacg ggtacgtcac cgtgctcgag cacgcagacg aactcgacag
     4561 gatctggcac gtccgcgggg agcgcgtgat cctcgcgacc ggcgcgttcg agcgttcgat
     4621 cgcgttcgcc gacaacgacc ggcccggcgt gatgctcagc cacgccgcgt cgctgtacct
     4681 cgagcacggg ctgctcgtcg gctcgcgcac ggtcgtgttc accgcgcgcg caggggccta
     4741 cggcgcggcc tcgaacctcg tcgggctcgg cggcgaggtg cgggcgatcg cggacctgag
     4801 tgggaccgat cccgacccga ggctcgcgag ggacgccgag gtgctacgcg gctgggtcgt
     4861 ctctgggacc gacggcgacc gaaggctcga ggcggtccat ctccgagggc ccgacggtga
     4921 acgacggacg atcgagtgcg attcgctcct cgtgtcgggc ggctgggacc cgaacctcgc
     4981 gctctggcgc tcgatcggcg gcggggtgcg cttcgacgag ggggtaagcg cgttcgtccc
     5041 caccgaaggt ccgccgtggt tgtcggtggt cgggtctgcg gccggcgagg tcgtcaacgg
     5101 cgtgcccgtt tggttcgttc cggccgagga ttactcgcgg cacttcgtcg acttccaacg
     5161 cgaccagacg atcgcggaca tcgccg
//
LOCUS       DATBRK010000242         5334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_39704, whole genome shotgun sequence.
ACCESSION   DATBRK010000242 DATBRK010000000
VERSION     DATBRK010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39704"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VIH70_07345"
     CDS             <1..740
                     /locus_tag="VIH70_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8116212.1"
                     /translation="LGSSGTWPNADTATSGYLVQHDGFNMWMDAGTGTLANLQQYIDI
                     PDLHAIVISHEHPDHFVDLYPAFYAWHYGELGEPGLPVFVPTDFSQRLADLVSIDSQV
                     AMRTAFAFTEVAPGEAFEIGPFRVKTEPMAHLGLPALGFRVAVDGQVLAYTGDTGPTH
                     HVEVLARDADLLLAEATWQDRDDLLPFHMSSRQAADHARESGVGKLVLTHIWPSLDHE
                     ISKEQAAQEYPGPIDTAVEGMRFEVGS"
     gene            740..1456
                     /gene="rph"
                     /locus_tag="VIH70_07350"
     CDS             740..1456
                     /gene="rph"
                     /locus_tag="VIH70_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY8116213.1"
                     /translation="MVRPDGRATDELRPVAVELGFQEWAEGSVLFSMGRTKVLVAATV
                     QDEAPRWLRGSGSGWVTGEYAMLPRATTERTFREVNKGRPSGRTQEIQRLIGRSLRAV
                     TDLKRLGERTITIDCDVLVADAGTRCASITGGYIALALALRGLAERRGIEGDLLTDTV
                     AAVSVGLLDGHALLDLSYEEDSGAGVDCNVVMTGSGRLVEIQGTAEGEPFSRPDLDAL
                     VDLAEAGIKQLTQIQRDALA"
     gene            1460..2059
                     /gene="rdgB"
                     /locus_tag="VIH70_07355"
     CDS             1460..2059
                     /gene="rdgB"
                     /locus_tag="VIH70_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY8116214.1"
                     /translation="MPFPERLAIATKNPHKLRELGRICRDWPVEWLTVENHPGPWPDV
                     PEPHDTYLDNALEKARAIASALGEAAIADDSGIEADALDGGPGPRSARFAGEGASDDE
                     NLAELVEAIVDEPSDARTARYRCVAAIAWPDGRALHAEGVCEGTLVVTPRGAGGFGYD
                     PIFVPEGEHRTMAELEDEEKDRISHRGRAFRALAELLSA"
     gene            2187..3044
                     /locus_tag="VIH70_07360"
     CDS             2187..3044
                     /locus_tag="VIH70_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116215.1"
                     /translation="MSDPMHRGQQLRAFFVMLAMIFLVGAGAAYGATWYRQSGLEADA
                     TRDARRLSIDVLQPLLVTADTQGPVRGARYDELLAAVDERVLAGPVNGVLLLAQDGTV
                     LFADQPSLVGDHQPALRDDIHAMISGQSQSSVDGDRFHTLTVLEIGSPPTLVAAELVQ
                     SHQPIVEESREPWYPWFGRAIAAAIVCFGLAIVTATVFSVIGGLARRTARRRSAVAAP
                     RPRRKKADRVADENLPAYMQPSFKEEVAARGRVEEELNAVQHERDALATRVQHLEAEL
                     DRLKDRSAV"
     gene            complement(3102..3344)
                     /locus_tag="VIH70_07365"
     CDS             complement(3102..3344)
                     /locus_tag="VIH70_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEY8116216.1"
                     /translation="MDDQQIVERINELAHQEHQLWEAESRGEASETDRERLTELQVTL
                     DQCWDLLHQRRARRSAGMDPDDAQVRDEREVEGYLG"
     gene            3430..4814
                     /locus_tag="VIH70_07370"
                     /pseudo
     CDS             3430..4814
                     /locus_tag="VIH70_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
     gene            complement(4819..>5334)
                     /locus_tag="VIH70_07375"
     CDS             complement(4819..>5334)
                     /locus_tag="VIH70_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116217.1"
                     /translation="MLDLASTYTGDALEIRELVLPDDPIPKADAIVSIGHVLSHLSEA
                     DAIDRALVAIAEALRPGGLIALDLCDFSYAEARPDFPIRARVADEWAIITEPSLPSPD
                     TFIRHMTVFLRNEDGSWRRDDERHENVLINTSLVPGLLAQHGVRATVETSFGAGVQLQ
                     DGLHAIVGTKY"
BASE COUNT          753 a         1860 c         1920 g          801 t
ORIGIN      
        1 tcctcggctc gagcggcacc tggccgaacg ccgacacggc cacctccggc tacctggtgc
       61 agcacgacgg gttcaacatg tggatggacg ccggcacggg gacgctggcc aacctgcagc
      121 agtacatcga catccccgac ctgcacgcga tcgtgatctc gcacgagcac cccgatcact
      181 tcgtcgacct gtatccggcc ttctatgcgt ggcactacgg cgagctcggg gagccgggtc
      241 tgcccgtgtt cgtccccacc gacttctccc agcggctggc ggacctcgtg tcgatcgact
      301 cgcaggtggc gatgcgcacg gcgttcgcgt tcacggaggt ggcgcccggc gaggcgttcg
      361 agatcggccc gttccgcgtc aagactgagc cgatggcgca cctcgggttg cccgcgctcg
      421 gctttcgggt cgcggtggac ggtcaggtac tcgcgtacac gggcgatacc ggccccaccc
      481 accacgtcga ggtgctggcg cgcgacgccg acctcctgct cgccgaggcg acctggcagg
      541 accgcgacga cctcttaccg ttccacatgt cctcgcggca ggcggccgac catgcgcgcg
      601 aatccggggt cggcaagctc gtgctcaccc acatctggcc gtcgctcgat cacgagatct
      661 cgaaggaaca ggcggcccag gagtaccccg gcccgatcga caccgccgtc gagggcatgc
      721 gcttcgaggt cggttcgtga tagttcggcc cgacggccgt gcgaccgacg agcttcgacc
      781 ggtcgccgtc gagctcgggt tccaggagtg ggccgagggg tcggtgttgt tctcgatggg
      841 ccgcacgaag gtgctcgtcg ccgcgacggt ccaggacgag gcgccccggt ggcttcgggg
      901 cagcgggagc ggctgggtca ccggcgagta cgcgatgctc ccgcgcgcga cgaccgaacg
      961 caccttccgc gaggtgaaca agggtcggcc gagcgggcgg acgcaggaga tccaacggtt
     1021 gatcggccgc tcgctccgag ccgtcaccga tctgaagcgc ctgggcgagc ggacgatcac
     1081 gatcgattgc gacgtgctgg tcgcggacgc gggaacccgg tgcgcctcga tcaccggcgg
     1141 atacatcgcg ctcgccctcg cgcttcgcgg cctggccgaa cgcaggggca tcgagggcga
     1201 cctcctgacc gacacggtcg ccgccgtgag cgtcgggctc ctcgacggcc acgcgctgct
     1261 cgatctctcg tacgaggagg actcgggcgc cggggtggac tgcaacgtgg tgatgacggg
     1321 ttcggggcgg ctggtcgaga tccaggggac cgccgagggc gagccgttct cccggcccga
     1381 cctcgacgcg ctggtcgatc tggccgaggc cgggatcaag cagttgacgc agatccaacg
     1441 cgacgcgctc gcgtgacccg tgcccttccc ggaacgcctc gcgatcgcga cgaagaaccc
     1501 ccacaagctc cgcgagctcg gtcgcatctg ccgtgactgg ccggtcgagt ggctcaccgt
     1561 cgagaaccat ccagggccct ggcccgacgt tcccgaaccc cacgacacct acctcgacaa
     1621 cgccctggag aaggcgcggg cgatcgcgtc ggcgctgggc gaggccgcga tcgccgacga
     1681 ctccggcatc gaggccgacg cgctcgacgg cgggcccggg ccgcgctcgg ctcggttcgc
     1741 cggggagggg gcgagcgacg acgagaacct cgcggagctg gtcgaggcga tcgtcgacga
     1801 gccgtccgat gcccggaccg cccgctaccg gtgcgtggcc gcgatcgcct ggcccgacgg
     1861 ccgggcgctg cacgccgagg gcgtctgcga gggcactctc gtcgtgacgc cgcggggagc
     1921 ggggggcttc ggctacgacc cgatcttcgt ccccgaaggc gagcaccgga cgatggccga
     1981 gctcgaggac gaggagaagg acaggatcag ccaccgaggt cgagccttcc gagccctggc
     2041 ggagctcctg tccgcgtagc gccgggcggg atccggcccg gcctcgtctc ggcgctccgc
     2101 gaggacccca ccgttcccgg atcgcgctca ggttccggcc cccgactgcc gataacccgt
     2161 ggcaggactc agggacgggg gcacccatgt ccgatccgat gcaccgtggg cagcagctgc
     2221 gagcgttctt cgtcatgctc gcgatgatct tcctcgtggg cgcgggcgcg gcctacggcg
     2281 cgacctggta ccggcagagc gggctcgagg ccgacgcgac ccgggacgcg cgcaggctct
     2341 cgatcgacgt gctccagcct cttctcgtca ccgccgatac acagggtccc gtgcggggcg
     2401 ctcgctacga cgagctcctc gccgcggtcg acgaacgcgt gctcgccggt ccggtcaacg
     2461 gcgtgctgct gctggcccag gacggcaccg tcctgttcgc cgatcagcca tcgctcgtcg
     2521 gagaccacca gccggccctg cgcgacgaca tccacgcgat gatctccggc cagtcgcaaa
     2581 gctcggtcga cggcgatcgg ttccacacgc tgacggtgct cgagatcgga tcgccgccga
     2641 cgctcgtggc cgccgagctc gtccagtccc accagccgat cgtcgaggag tccagggagc
     2701 cctggtaccc gtggttcggg cgggcgatcg ccgccgccat cgtgtgcttc ggcctcgcca
     2761 tcgtgacggc gaccgtcttc tccgtgatcg gtgggctcgc gcgtcgtacg gcgcggcggc
     2821 gttcggcggt cgcggcgccg aggcctcgcc ggaagaaggc ggaccgcgtc gcggacgaga
     2881 acctccccgc gtacatgcag cccagcttca aggaagaggt cgcggcccgg ggccgggtcg
     2941 aggaagagct gaacgccgtc cagcacgagc gcgacgcgct cgccacgcgc gtccagcatc
     3001 tggaggccga gctcgaccgc ctcaaggacc gctcggccgt ctgaccgaag ctcgcccccg
     3061 ccgcgcaacc cggtcgaacg actccagggg cgatacgggc ctcagcccag gtacccctcc
     3121 acctcacgct cgtcccgaac ctgcgcgtcg tccggatcca tccccgcgct gcgccgggcc
     3181 cggcgttggt ggaggaggtc ccagcactgg tcgagggtga cctggagctc cgtaaggcgc
     3241 tcgcggtcgg tctcgctcgc ctcgccgcga gattcggcct cccagagttg atgctcctgg
     3301 tgcgctagct cgttgatccg ctcgacgatc tgctggtcat ccatgtcgac tcccgtccga
     3361 ggtcaggccc caacgatacg accgtcctgc cggtacggac ggcatcgccc ggtgcgcgat
     3421 gatgggccga tgtcgaacct gagagagctg ctgacgagca ccgccgaccg ggtcgcggac
     3481 taccgagggt ccctcgccga acgccgctgg ctccgttgtc ggaccacgaa cgcctccgcg
     3541 cggagctcgg cggcccgctg ccggtcgagg gagccgacgc ggggaccgtg ctcgacgagc
     3601 tcgtcgccgc gatcgaaccg gcgctgatgt cctcggccgg tcctcgcttc ttcggcttcg
     3661 tcgtcggggg atcgctcgat gccgcggtcg gcgcggacat cctcacgacc ggctgggacc
     3721 agatggcgtt caacggcgcg tcgtcgccgg ccgcggcgtt ggtcgaggag atcgcgggcg
     3781 cgtggctgaa agagtcgctg ggtctgccgg cggacgcgtc gttcgggatc gtgacgggaa
     3841 cgcaggcggg gaacacggtc gggctcgcgg cggcgcgcca ccgcgtgctc cagcgcgtcg
     3901 ggtgggacgt ggagcgacac ggtctgcacg gcgcgcccag gatccgcgtc gtcgccggcg
     3961 aggaacgcca cgcgacgatc gaccgctcgc tccgcctgct cggcatgggg gtcgacgcgg
     4021 tccagagcgt tccggccggt ccgaacggcg cggtcgatcc cgacgccctc gtccgaatac
     4081 tcgaggccga tccggacgag ccgacgatcg tgtgtggccc aggccgggaa cgtgaacacg
     4141 ggggcgttcg acgaccttct cgtcgcgtgc gaggcggcgc gccggcgggg cgcctgggtc
     4201 catgtcgacg gggcgttcgg cctgtgggcc gcggcgagcc cggtgttcga gcatctggtc
     4261 gtcggtcgtg aagcggccga ctcctgtgtg gtcgatggcc acaagtggct caacctgccg
     4321 tacgacgcgg gcttcgtgtt ctgcgcggat caggacgcgc acgctgcgtc gatgtcgtac
     4381 acggccgcgt atctcgtcgg cagggcgagg ggcctgttcg ggcaccggcg gactacgtgc
     4441 ccgagtcctc ccgccgcgct cgggggttcg tgacgtgggc cgcgctgcgc gagctcggcc
     4501 gcgatgggct cgccgacctg gtcgagcgct gctgcgcgct cgcgcggcgc ttcgccgagg
     4561 agctgggcgc tatcggtggt gtcacgatcg cgaacgacgt cgtgctcaac caggtcctgg
     4621 tggggttcgg cgacgacgag cgcaccgacc tggtgatcga gggcatccag cgcgagggca
     4681 cctgctggat gggcggcacg acctggcacg gccggcggct gatgcggatc tccgtctcca
     4741 actggacgac gacggaggcc gacgtcgacc gctcggtcga ggcgatccgg tgggtgctcg
     4801 cctcccagtc atagcgcgtc agtacttggt gccgacgatc gcatggagtc cgtcctgcag
     4861 ttgcacccca gcgccgaacg aggtctcgac cgtcgcccgg accccgtgct gggcgagtag
     4921 cccggggacg aggctcgtgt tgatcaggac gttctcgtgg cgttcgtcgt cgcggcgcca
     4981 ggagccgtcc tcgttccgca ggaacacggt catgtggcga atgaacgtgt ctggggaggg
     5041 caacgaaggc tcggtgatga tggcccactc gtcggccacc ctggcccgga tggggaagtc
     5101 cggccgggcc tcggcgtagc tgaagtcgca gagatccagc gcgatcaggc cgcccggtcg
     5161 cagggcctct gcgatcgcga cgagggctcg gtcgatcgcg tcggcttcgg agaggtggct
     5221 cagcacgtgc ccgatcgaga cgatcgcgtc cgccttcggg atcggatcgt ccggaaggac
     5281 cagctcgcgg atctccaatg cgtccccggt atacgtgctc gccaggtcga gcat
//
LOCUS       DATBRK010000243         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1771829, whole genome shotgun sequence.
ACCESSION   DATBRK010000243 DATBRK010000000
VERSION     DATBRK010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771829"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /gene="prfB"
                     /locus_tag="VIH70_07380"
     CDS             complement(<1..1042)
                     /gene="prfB"
                     /locus_tag="VIH70_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY8116218.1"
                     /translation="MARGWTTTPRRSPSSPSESRRPRSSFDVDAKRSKAAELERDASD
                     PSLWDDAARGQRLTTTLAGLRAEIERYDALVRRLEDARTMDELLEGEADREMATELAR
                     AARELERDVEKLELATLLGGEYDDHDAVATVHAGAGGTESQDWAEMLLRMYLRWAERE
                     KLDVEVEEMLYGDEAGIKSATFIVHGPYAYGLLSAERGVHRLVRISPFDAQKRRHTSF
                     ANLDVIPLLEGTADEDISIDPKDLRIDVYRSTGPGGQSVNTTDSAVRITHLPSGITAA
                     CQNERSQLQNRAVAMRILKARLAERMRQEQQTKLENLRGERKGIDFGSQIRSYVLAPY
                     RLVKDHRTNVEIG"
     gene            1203..1436
                     /locus_tag="VIH70_07385"
     CDS             1203..1436
                     /locus_tag="VIH70_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116219.1"
                     /translation="MATRSASLPIEQTYLGRARLAVVAIVLVAAVVVTIAVALGRSTP
                     AGGTGPGPVLDHGPVTVHNEPIVVNGSLCGQCR"
     gene            complement(1508..>2444)
                     /locus_tag="VIH70_07390"
     CDS             complement(1508..>2444)
                     /locus_tag="VIH70_07390"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY8116220.1"
                     /translation="GDPGESRFYLSLEDDLMRMFASDRVSSIMDRLKWPEDEPIAARM
                     VTRAVESAQRQIEELNFERRKNILKYDDVMNTQRQVIYEEREKLLSGEDFKDQAIRMV
                     EEVVRRTVQRLAPEEVFNEEWDHELVLTGLTEVFPTTISADDLEEMVSSSELEERSVE
                     DALERYEAKEQALGSQTMRELERLVLLNITDTKWREHLYEMDYLQEGIHLRAYAQKDP
                     LTEYRREAYLMFEELTESIQDDFVKYIYRVELVRQDEPGRPRPQRVQTTHGDGDQAQG
                     DAGAGVQARSGKVPRNAPCPCGSGKKYKKCHGAVA"
BASE COUNT          330 a          882 c          792 g          440 t
ORIGIN      
        1 cgccgatctc gacgttggtc cggtggtcct tgacgagccg gtacggcgcc aggacgtagg
       61 agcggatctg cgagccgaag tcgatcccct tgcgttcgcc ccggaggttc tcgagcttgg
      121 tctgctgctc ctgccgcatc ctctcggcca gacgggcctt gaggatccgc atcgccaccg
      181 cgcggttctg cagctgcgag cgctcgttct gacacgccgc ggtgatcccg ctcgggaggt
      241 gcgtgatccg gaccgcggag tccgtcgtgt tgacggactg cccgcccgga cccgtcgagc
      301 ggtagacgtc gatccgcaga tccttcgggt cgatcgagat gtcctcgtcg gccgtcccct
      361 cgagcagcgg gatcacgtcg aggttggcga acgacgtgtg ccgccgcttc tgcgcatcga
      421 acggcgagat ccgaaccagc cggtgcacgc cgcgctccgc ggacaggagg ccgtacgcgt
      481 acggcccgtg gacgatgaag gtcgcgctct tgatgccggc ctcgtcgccg tacagcatct
      541 cctcgacctc gacgtcgagc ttctcccgct cggcccatcg caggtacatc cgcaacagca
      601 tctccgccca gtcctgcgat tcggttccgc cggcacccgc gtgcacggtg gcgaccgcgt
      661 cgtggtcgtc gtactcgccc ccgaggagcg tcgcgagctc gagcttctcc acgtcgcgtt
      721 cgagctcgcg ggcggcgcgg gcgagctcgg tcgccatctc gcgatcggcc tcaccctcga
      781 ggagctcgtc catcgtccgc gcgtcctcga gccggcggac cagcgcgtcg tatcgttcga
      841 tctcggcccg caggcctgcg agggtggtcg tcagccgttg gccgcgggca gcatcgtccc
      901 agagcgacgg atccgaggca tcgcgctcca gctcggccgc cttcgaacgc ttggcgtcga
      961 cgtcaaagga actccttggc cgacgcgatt cgcttggcga gctcggcgag cgtctgggtg
     1021 tggtcgtcca tcctcgcgcc atcgtaacgg ctccgacaag gagccccagg cccgtcggag
     1081 ccggggcgtc cgcgcgtgtg tgtcgcacgt caccttcgag gggtgccgtg gaccaccgaa
     1141 cgcccggcgc cggccaccgt acggttcgtt cgacaaacgg accgcgacac ggagggaacg
     1201 agatggccac caggagcgcg agcctcccga tcgaacagac ctacctgggc agggcgcggc
     1261 tcgccgtggt ggcgatcgtg ctcgtggccg cggtggtcgt gacgatcgcc gtcgcgctcg
     1321 gccgctcgac ccccgccggc ggcaccggac cgggccccgt cctcgaccac ggtccggtca
     1381 cggtccacaa cgagccgatc gtcgtgaacg ggagcctttg cggacagtgc cggtgaggta
     1441 acccccctct ccaggcgcga aggaggccgg cccatcaggg ccggcctctt tcactcgcga
     1501 gggctcgtta cgcgaccgcg ccgtggcact tcttgtactt cttgccgctc ccgcacgggc
     1561 acggcgcgtt ccgcggcacc ttcccgctcc tggcctggac cccggcgccg gcgtcgccct
     1621 gggcctggtc cccgtccccg tgtgtggtct gcacgcgctg gggtctgggg cgaccgggct
     1681 cgtcctgacg cacgagctcc acgcggtaga tgtacttgac gaagtcgtcc tggatcgact
     1741 cggtcaactc ctcgaacatc aggtacgcct cacgccggta ctcggtgagc gggtccttct
     1801 gggcgtacgc ccgaaggtgg atcccctcct gcaggtagtc catctcgtac agatgttcgc
     1861 gccacttcgt gtccgtgatg ttcagcagga cgagtcgctc gagctcccgc atcgtctgcg
     1921 accccagcgc ctgctccttc gcctcgtacc gctcgagcgc gtcctcgacg ctgcgctcct
     1981 ccagctcgga cgagctgacc atctcctcca ggtcgtcggc cgagatcgtc gtcgggaaca
     2041 cctcggtgag gccggtcagc acgagctcgt gatcccactc ctcgttgaag acctcctccg
     2101 gcgcgagccg ttggaccgtc cggcggacga cctcctcgac catccggatc gcctggtcct
     2161 tgaagtcctc gcccgagagc agcttctcgc gttcctcgta gatcacctgg cgctgggtgt
     2221 tcatgacgtc gtcgtacttc aggatgttct tgcgtcgctc gaagttcagc tcctcgatct
     2281 gtcgctgggc gctctccacg gctcgcgtga ccatccgggc cgcgatgggc tcgtcctcgg
     2341 gccatttcag ccggtccatg atggacgaca cgcgatcgga ggcgaacatc cgcatcaggt
     2401 cgtcctcaag cgagaggtag aaccgcgact ccccggggtc cccc
//
LOCUS       DATBRK010000244         4029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2203095, whole genome shotgun sequence.
ACCESSION   DATBRK010000244 DATBRK010000000
VERSION     DATBRK010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203095"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="VIH70_07395"
     CDS             complement(<1..1486)
                     /locus_tag="VIH70_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8116221.1"
                     /translation="MAGAPRRNRGSWRDADPLWSKAPLVLLRYPGLFVSIAVGALLLA
                     LAASAYPLFISSSASELVAARIHDPSYTRWAVGMMYRNGALPLPGPDRNGDVEPRVDR
                     IFEGLVAPSPYLGDPLESALGPIAAISVAGEHEQRDTRLFMGEQAPDHVEILAGSARD
                     GVLVPDLISDALDITPGEKIVIGSPDEGTVTLPVGGTYRSLYKGGASGYWRPWNDALV
                     LYCGNCAPPPQAVIVPKDRFADAARAIGLDHAAFAWQAPVLRDLTLQEAEDAAAISSR
                     ISEEIDDRALLDRCFAGFFCNRSNGPVWGSSMDDVVHDVHGRLVAIDGPARLLRIAGM
                     LVALVVVAGAGAFAMAARRVESNLLFARGARPRAVGGRSALEAVIPCAIGAALGLGLA
                     FALVRWVGPGGALAVSASGQAAWAAAASGVVAVAAIAIVSTVSFLRQSEHHRSRLRFV
                     GAFPWELVLIGLSLLILQRLRSGGAVVVDRSLGTETPSLLLFAFP"
     gene            1635..2648
                     /gene="hemE"
                     /locus_tag="VIH70_07400"
     CDS             1635..2648
                     /gene="hemE"
                     /locus_tag="VIH70_07400"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY8116222.1"
                     /translation="MNDRFLRALRREPVDRTPVWFMRQAGRYLPEYRELRGDRDILEA
                     IREPEVAVELTLQPLRRMPLDAAIVFADIMVPVAAIGVPVRIEAGVGPVVADPIRDAS
                     GVARLRALEPDADEPYVAETIRLLRKELRVPLIGFAGAPFTLASYLVEGGPSRDHVRT
                     KALMHDEPSTWSALMDALATLSRAHLLAQIEAGAQAVQLFDTWAGSLAPSDYQRSVRP
                     WSARVLENLPVPSIHFGVGTGDLLSSMRDAGGDAIGVDWRVPLDRAWERIGAGRSIQG
                     NLDPAVCVASWDAVERAATEILDSAAMRDGHVFNLGHGVLPHTPVENLQRLVDLVHER
                     TAR"
     gene            2645..3616
                     /gene="hemH"
                     /locus_tag="VIH70_07405"
     CDS             2645..3616
                     /gene="hemH"
                     /locus_tag="VIH70_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY8116223.1"
                     /translation="MSSPPIGVLVMAYGTASGPDDVERYYTDIRGGRRPSPEHLEELR
                     ERYAAIGNRFPLLDITRQQAEGLERELNRGEDGAFRAYLGMKHSPPFIAETIDRMRED
                     GIERAIGIVMAPHWSGMSVESYLDRVREQAGEGPPAFDFVRSYHDDPRFVSFLAARVA
                     DALSGLTAEERRGATVIFSAHSLPTRTVEDGSLRCLRCHACEDACRYRDGLAETADLV
                     MAELGLARHTIAWQSAGRTDDPWWGPPIEDVLADLASQGCPAAVVCSAGFVADHLEIL
                     YDLDIEARAIAERAGMRLVRTEMPNADPAFVRALADVVRERLTASRT"
     gene            3622..>4029
                     /locus_tag="VIH70_07410"
     CDS             3622..>4029
                     /locus_tag="VIH70_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8116224.1"
                     /translation="MAPTAARVVVVGGGVSGLTAAHRLLEADPALELAVLDSEPDVGG
                     RLRTATVGDLELESGPDSFVARKPWAVDLCRELGLELTAPGARDALIWTERGLVPLPE
                     SALGIPTDVEEIVRWPGLSRRGRVRALAELVRKA"
BASE COUNT          572 a         1491 c         1437 g          529 t
ORIGIN      
        1 gcgggaacgc gaacagcagc aggctcggcg tctccgtccc gagcgagcga tcgaccacga
       61 ccgcgccccc gctccgcagc cgttgcagga tcagcagcga gaggccgatc aggaccagct
      121 cccacgggaa ggcacccacg aagcgcagcc ggctgcgatg gtgctcggac tggcgcagga
      181 acgagacggt cgagacgatc gcgatcgccg cgacggcgac gacccccgat gccgcggccg
      241 cccacgccgc ctggcccgat gcggatacgg ccagcgcgcc gcccggaccc acccaccgta
      301 cgagcgcgaa cgcgaggccc aacccgagcg ccgcgccgat cgcgcacggg atcacggctt
      361 cgagcgccga acgcccaccc accgccctgg gtctcgcgcc gcgcgcgaac aggaggttgg
      421 actcgacccg acgggcggcc atcgcgaacg cacccgcgcc ggcgaccacc accagcgcga
      481 cgagcatccc ggcgatccgc aacagccgcg ccgggccgtc gatcgcgacg agccgcccgt
      541 ggacgtcgtg gacgacgtcg tccatcgagc tcccccacac cggaccgttc gaacggttgc
      601 agaagaaccc ggcgaagcat cggtccagca gcgcccggtc gtcgatctcc tcggaaatgc
      661 gggaggagat cgccgccgcg tcctcggcct cctggagcgt gaggtcgcgc aggacgggcg
      721 cctgccacgc gaacgccgcg tggtcgagcc cgatcgcccg cgccgcgtcg gcgaaccgat
      781 ccttcggcac gatcaccgcc tggggaggag gcgcgcagtt cccgcagtac aggaccaggg
      841 cgtcgttcca gggccgccag taccccgagg ccccgccctt gtacagggac cggtaggtcc
      901 cgccgaccgg cagcgtcacg gttccctcgt cgggcgagcc gatcacgatc ttctcgcccg
      961 gcgtgatgtc gagggcatcc gagatcaggt cggggaccag cacgccgtcg cgggccgaac
     1021 ccgcgaggat ctcgacgtgg tccggcgcct gctcccccat gaacaggcgc gtgtcccgct
     1081 gctcgtgttc gccggcgacc gagatcgccg cgatgggccc gagcgccgac tccaaggggt
     1141 cgccgaggta cgggctcggg gcgacgaggc cctcgaagat ccggtcgacc ctcggctcga
     1201 cgtcgccgtt gcggtccggc ccaggcagcg gcaacgcccc gttgcggtac atcatcccga
     1261 ccgcccagcg cgtgtacgag gggtcgtgga tccgggccgc gaccaactcg ctcgcggagg
     1321 acgagatgaa gagcgggtag gccgacgcgg cgagggcgag caggagcgcg cccaccgcga
     1381 tcgagacgaa cagccccggg taccgcagca ggacgagcgg ggccttcgac cacagtggat
     1441 cggcgtccct ccacgagccg cgattccgtc tgggtgctcc cgccatcggc ctcccgtatg
     1501 cgttcgtcag taggttctac gcccgaggag ggccgggcgt tcgtgccgct tggcatgtcc
     1561 gccccaaatg ccgcccgtcc gtcgcccagc gggcccgtcc gactcccacc gtacccgtcc
     1621 gtatcctggt gaccgtgaac gaccgattcc tgcgcgcgct ccggcgcgaa cccgtggacc
     1681 ggacaccggt ctggttcatg cgccaggcgg gccggtacct gccggaatac cgggaactcc
     1741 gcggcgaccg ggacatcctc gaggcgatcc gcgagcccga ggtcgccgtc gagctcacac
     1801 tgcagccgct ccggcggatg ccgctggacg ccgcgatcgt gttcgccgac atcatggtgc
     1861 cggtcgccgc gatcggggtg cccgtgcgga tcgaggcggg cgttggtccg gtcgtcgccg
     1921 atccgatccg ggacgcgtcg ggcgtcgcgc ggctccgggc gctcgaaccc gacgcggatg
     1981 agccctacgt cgcggaaacg atccgcctcc tccgcaagga gctgagggtc ccgctgatcg
     2041 ggttcgccgg cgcgccgttc acgctcgcga gctacctggt cgagggcggc ccgtcgcggg
     2101 atcacgtccg gaccaaggcg ctgatgcacg acgagccctc cacgtggtcg gcgctgatgg
     2161 acgcgctcgc gacgctctcg cgcgcccacc tcctcgcgca aatcgaggcg ggtgcgcagg
     2221 cggtgcagct gttcgacacg tgggcgggat cgctcgcgcc gagcgactac cagcgatcgg
     2281 tccggccgtg gtcggcgcgg gtgctcgaga acctccccgt gccctcgatc cacttcggcg
     2341 tggggaccgg cgacctcctg tcgtcgatgc gcgacgccgg cggcgacgcg atcggcgtcg
     2401 actggcgggt cccgctcgac cgagcctggg agcggatcgg cgcgggccgc tcgatccagg
     2461 gcaacctcga tcccgccgtc tgtgtcgcgt cctgggacgc ggtcgaacgt gccgccaccg
     2521 agatcctcga cagcgcggcg atgcgcgacg gccacgtgtt caacctcggt cacggggtcc
     2581 ttccgcacac gccggtcgag aacctccagc gcctcgtgga cctcgtgcac gagcggaccg
     2641 cccgatgagc tctccgccga tcggcgtgct cgtgatggcg tacggcaccg cgagcggccc
     2701 cgacgacgtc gagcgctact acacggacat ccgcggcgga cgacgtccgt ccccggagca
     2761 cctcgaggag ctgcgcgaac gctacgcggc gatcggcaac cgcttccccc tgctggacat
     2821 cacccggcag caggccgagg gcctggagcg ggaactcaac cgcggcgagg atggcgcgtt
     2881 ccgcgcctac ctggggatga agcactcgcc cccgttcatc gccgagacga tcgaccgcat
     2941 gcgcgaggac ggcatcgaac gcgccatcgg gatcgtgatg gcgccgcact ggtcggggat
     3001 gtccgtggag agctacctcg accgcgttcg cgagcaggcg ggcgagggtc cgcccgcgtt
     3061 cgacttcgtc cgctcgtacc acgacgaccc ccggttcgtg tcgttcctgg cggcgcgggt
     3121 ggccgacgcg ctcagcgggc tcaccgccga ggaacgccgg ggcgcgacgg tgatcttctc
     3181 ggcgcacagc ctgcccaccc ggacggtcga ggacggctcg ctccggtgcc tgcgatgcca
     3241 cgcgtgcgag gacgcctgcc ggtaccgcga cggcctcgcc gagaccgccg acctcgtgat
     3301 ggccgagctc ggcctcgctc gacacacgat cgcgtggcag agcgcgggcc gtacggacga
     3361 cccgtggtgg ggtccgccga tcgaggacgt gctcgcggac ctcgcgtcgc aggggtgtcc
     3421 ggccgccgtc gtgtgctcgg ccgggttcgt ggccgaccac ctcgagatcc tgtacgacct
     3481 cgacatcgag gcgcgggcga tcgccgagcg ggccggcatg cgtctcgtgc gcaccgagat
     3541 gccgaatgcg gatcccgcgt tcgtccgggc gctcgccgac gtggtccgcg agcggctcac
     3601 ggcctcgagg acctgaggat ggtggcgccg accgccgcgc gggtcgtggt cgtgggcggc
     3661 ggggtctcgg gcctgacggc ggcacacagg ctgctcgagg ccgatccggc gctcgagctc
     3721 gccgtgctgg attccgagcc cgacgtcggc ggccggcttc ggaccgcgac cgtcggcgac
     3781 ctcgagctcg agtccggtcc ggactcgttc gtcgcccgca agccgtgggc cgtggacctg
     3841 tgccgggagc tcggtctcga gctgaccgcg cccggcgccc gcgatgccct catctggacc
     3901 gagcgcgggc tcgtcccgct tccggagagc gcgctcggca tccccacgga tgtggaagag
     3961 atcgtgcgct ggccgggcct gtcgcgccgg ggccgcgtac gcgcgctcgc cgaactcgtg
     4021 cgcaaggca
//
LOCUS       DATBRK010000245         9351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2495367, whole genome shotgun sequence.
ACCESSION   DATBRK010000245 DATBRK010000000
VERSION     DATBRK010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2495367"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VIH70_07415"
     CDS             complement(<1..918)
                     /locus_tag="VIH70_07415"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY8116225.1"
                     /translation="MHPAIHPIVEVGVKSGLEIAQEAELRPITEIAASAGIPDDVLEP
                     FGRHRAKVDLSILDGLADRPDGKLIITTAITPTKAGEGKTTTSISLAQGMGKIGKDVV
                     LCLREPSMGPVFGIKGGGTGGGFAQVVPMEDINLHFNGDFHAVTAAHNLLASALDASL
                     YFGNPLGVDPTSITWPRTLDVNARELRYTVIGLGGKMHGVPRENGFVITAASEVMAVL
                     ALASDLADLRARLGRIVVASTGDGEPVTAEQLGVAGAMTVMMKDALKPNLVQTLEGQP
                     VLIHTGPFGNVAHANNSIIEDRVGLKLADY"
     gene            978..2840
                     /locus_tag="VIH70_07420"
     CDS             978..2840
                     /locus_tag="VIH70_07420"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEY8116226.1"
                     /translation="MAVRYSGPMDIPSFLRMYPPFDDLDDDRLAEVVRHAHIEFYPEG
                     TVILQESGEPAGFLHVVRRGAVEVLEGGDVVDIQGEGEVFGFVSLLTGASPLVTIRAY
                     EDVICYLIDREIGEEIMSSRRGMVFLASAFRRRDVRALEHVARGTVDDSPEPVAALVR
                     RRPLSLPANASIRQAAEIMATEREGFVLLEGVGERGLSIVTDRDLRSRVLAEGRDPTT
                     AAAEVASSPVVSVPAQATVAEVTALMLERGVHHVPVEDGDGGFLGVVTDIDLLAAEQH
                     RAFGLKKDIELAHDRESAIEAFRSLPEVATRLVEASVEPLEIAHIVAVTIDALTVRLL
                     EIGWAEIGDPPCPWTWLALGSEARQEQGLFTDQDNALIFDPVAIGEEEADAYFGQLGA
                     IVNDGLEEAGIPRCRAGVLAGNRDWRGTVRTWQNRFRGWIEDPGRTGSAFTGIAIDYR
                     PVTGPLDARLHLDEIIRSAREQHDFIRHLGAHAVASRPPKGLLKDGVVQGKRRTGRLD
                     IKAVGIGLITNVARLLAVMSGLTENRTTLRLRDTTALGWLTEDESQGLVEAFELMWQV
                     RLEHQSRCVERGVAPDDLVDPRELGPLTRQALKEAFRMIDGAQDRLAALLGLRR"
     gene            2837..3493
                     /locus_tag="VIH70_07425"
     CDS             2837..3493
                     /locus_tag="VIH70_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8116227.1"
                     /translation="MSGARPAPPPRRDRRLPWRRLELASLDFEATGLDFARDTIVSFG
                     VVPIRAGRIDVGDAVYELVDPGEVVPSPASVTVHGLRPVDLRGARGLDAARAALAPVI
                     GGRFLVTWARRVEAGFLDVMYGGGVRSWLRRCIDARELMFALDDDPPVPLTLTSVAER
                     LGVPVASPHHAMDDALVTAQVFLVAGARLEASGVRTVRALQRTRRPESPALRRPRAPI
                     "
     gene            complement(3467..3958)
                     /locus_tag="VIH70_07430"
     CDS             complement(3467..3958)
                     /locus_tag="VIH70_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8116228.1"
                     /translation="MVAAPRRMPDPPERILIATVSQPFPREVLARAIELATPAHAKIT
                     VLGIAKVYGTSLGLPNPGLQPNRIEWQVLKDGTEEAADELRSRGFEVRVGLSRSRNAP
                     KMIAKWAIARNFHAIVVADPERPRWRTLLEGDVAHEIGRRCRVPVHAVRIASSSDRRA
                     RPA"
     gene            complement(3993..5510)
                     /locus_tag="VIH70_07435"
     CDS             complement(3993..5510)
                     /locus_tag="VIH70_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8116229.1"
                     /translation="MAKQDEQQFVRVEDYVKGELSKDTNWWGAFVVGLAGTILVTGVT
                     PAVLAPLGAAAIPNFIFWALTGWLLCLYLAEMAAMLPDRTGGAPAYAYYAFKDRLPRT
                     YPHINGVTVWMYWLGWMPVMAVNMILTGAYLPVLFGFELSGWWAQTHTLFDASLPVTN
                     FTIVIGAVLSVLLFVIAYLGIRFGTAAATVLGLLSMIPLTLIAVAPFFTGDVTGSNLW
                     PANLPDGTAFFSGTAFYVFLQFSALYTWNAIAMEAAACYIGECRNPDRDAPIAMNLSG
                     GYGTFIYTFLPMAVLGVLGLKFIQESLDPNAILVETAVRSLGSGFSKIVTSALIIALA
                     LSSLNAIMGCARSLYMMALDGQSPRIFGRVNRHNVPDFSMGLNTVLNILLIFVGAPGS
                     IYVLSNVGYVGSFVPVLFGYYFLRRWRPEIFRPFRLPEWMKYGAIALAVLYFVVWSVG
                     IPACAIEGCQLGGGKMFIIGVIIVLLYFPLYWWRRAEDRRLGTTDFRRVSVSQDV"
     gene            complement(5610..6977)
                     /gene="glnT"
                     /locus_tag="VIH70_07440"
     CDS             complement(5610..6977)
                     /gene="glnT"
                     /locus_tag="VIH70_07440"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HEY8116230.1"
                     /translation="MAERPGSVEQVRKLVDDMGIEFVFAQFVEMYGKPNAKLVPAAHL
                     EDVFAEGAGFAGFATGELGQGPHDPDIAAMPDPASFTPVPWQPNLARFACDVFVEGEP
                     WPYCPRTILKRSMAKARERGYVFKIGIEAEFFLTRLTEDGRIEVADPLDTLERPCYDM
                     KALSRQYDFITTLSKYETQLGWDNYANDHEDANGQFESNFVFDDAVTSSDRLIFFRYM
                     VHMLAQQRGLQATFMPKPFGHLTGNGLHFHMSLWDPDTDTNLFEDENDARGLGISELG
                     YHFLGGLLAHAKAYIGVTASTVNSYKRLIVGAPTSGATWAPAYVAYGRNNRTQMIRTP
                     APGRFEDRTIDGAANPYLAASAILAAGLDGIDNKLDPGDPNEGNMYETPVEELRRRGV
                     ELLPGNLLDAIRNLRQDDVLREGLGKVRGGDYLDYYCDVKEREWKEYHEGVSDWEVKK
                     YLSLF"
     gene            complement(7236..7646)
                     /locus_tag="VIH70_07445"
     CDS             complement(7236..7646)
                     /locus_tag="VIH70_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8116231.1"
                     /translation="MFEKLLLAVDGSPGSEKAVGIAAGLAKNLGSEVLVFHVREIEIG
                     RFSGQMEPPEEALELVNRVVSTLQEQGVVVKGEARSAPLGRAGREIVEGAAAAEVDGI
                     VLGSRGLSDWSALLLGSVAHKVISLAHCPVIVAR"
     gene            7810..9207
                     /locus_tag="VIH70_07450"
     CDS             7810..9207
                     /locus_tag="VIH70_07450"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY8116232.1"
                     /translation="MTTDIEAFIGQEGRDKAIQEVRKQIDAAGVEYMYYQFVSVTGRI
                     MGKGIPAAHWENISNKGFQLVYGSTANLFVDRQGNYIGYGPEARELVGIPEPETFCVF
                     PWDNKTARVFCTLFRGREEEVDGGAFLTSDCRGNLKRLHTEFEQETGLHLRAGCEPEM
                     MWLKVDADGKPTVEGKTKPYCYHIDQFSELQPLIHKVMEYGRAMGLDMIQGDHEDAPG
                     QLELNFMFDRAEATADRLTTYRQICRQVGREMGAFACFMPKPFMGVSANGCHHNISLW
                     QGDENMFMPEGDNPRMPGKIGLHSIGGVLEHLSALTAITAPTVNSYRRFWDAGFWAPV
                     FADWGFQNRTAALRISAPGRFEYRSVDSSVNPYLSFAALLAAMKDGIDRSLDPGEPEE
                     RNIYDAIAGGKDVKKVPHDLGAALDALRADPVIQGALPNEMYRVFMHYKQDEWDRFRA
                     TVSEWDIETYLDVLP"
     gene            9227..>9351
                     /locus_tag="VIH70_07455"
     CDS             9227..>9351
                     /locus_tag="VIH70_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY8116233.1"
                     /translation="MCGIAGVIHRDGSADIGSEMTSMLQSMKHRGPDSSGYALFGP"
BASE COUNT         1507 a         3143 c         3224 g         1477 t
ORIGIN      
        1 gtagtcggcg agcttcagcc cgacgcggtc ctcgatgatc gagttgttcg cgtgggcgac
       61 gttgccgaac ggaccggtgt ggatcaggac gggctgcccc tcgagcgtct gcaccaggtt
      121 gggcttcagc gcgtccttca tcatcacggt catcgccccg gcgacgccta gctgctcggc
      181 ggtgaccggc tctccgtcgc ccgtcgacgc gacgacgatc cgcccaagac gggcgcggag
      241 gtcggcgaga tcggaggcga gggcgagcac ggccatcacc tcggacgccg cggtgatcac
      301 gaacccgttc tctcgcggga cgccgtgcat cttcccgccg aggccgatga cggtgtagcg
      361 gagctcgcgc gcattgacgt cgagcgtccg cggccacgtg atgctggtgg ggtcgacgcc
      421 cagcgggttg ccgaagtaga gcgaggcgtc gagcgcggac gccagcaggt tgtgggcggc
      481 cgtgacggcg tggaagtcgc cgttgaaatg gaggttgatg tcctccatcg ggacgacctg
      541 cgcgaagccg ccgccggtgc cgccaccctt gatgccgaac acggggccca tcgagggttc
      601 gcgcaggcag agcacgacgt ccttgccgat cttccccatc ccctgcgcga gcgagatcga
      661 cgtcgtcgtc ttgccctcgc cggccttcgt cggcgtgatg gcggtggtga tgatcagctt
      721 gccgtccgga cggtcggcca gcccgtcgag gatcgagagg tcgaccttgg cgcggtgccg
      781 gccgaacggt tcgagtacgt cgtcggggat cccggccgac gcggcgatct ccgtgatggg
      841 ccggagctcc gcttcctggg cgatctcgag tccgctcttc acaccgacct ccacgattgg
      901 gtggatcgct gggtgcatcg tccggcggag gatacccgca ggtcccgcgc acgggaaagg
      961 cgaacgcgac cggcctgatg gcggtgcgat actcggggcc gatggacatc ccgtcgttcc
     1021 tgcggatgta tccgccgttc gacgatctcg atgacgatcg cctcgccgag gtggtgcgtc
     1081 atgcgcacat cgagttctac ccggagggaa cggtgatctt gcaggagtcg ggcgagccgg
     1141 ccgggttcct ccacgtggtc cggcgcggcg ccgtcgaggt cctcgagggt ggcgacgtcg
     1201 tcgacatcca gggcgagggt gaggtcttcg gcttcgtgtc cctgctgacc ggggcgagcc
     1261 cgctggtcac gatccgtgca tacgaggacg tcatctgcta cctgatcgac cgcgagatcg
     1321 gcgaggagat catgagctcc cgcaggggca tggtgttcct ggcctccgcg ttccggcggc
     1381 gcgacgtgcg agccctcgag cacgtcgctc gtgggaccgt cgacgacagc ccggaacccg
     1441 tggccgcgct cgtgcggcgt cggccgctct cgctgccggc gaacgcgtcg atccgacagg
     1501 ctgccgagat catggcgacc gaacgcgagg ggttcgtcct gctcgagggg gtcggggagc
     1561 gggggctctc gatcgtgacg gaccgcgacc tgcgctcgcg agtactggcc gagggacggg
     1621 accccacgac cgcggccgcc gaggttgcgt cctcgcccgt ggtctccgtg ccggcccagg
     1681 ccacggtcgc cgaggtgact gccctcatgc tcgagcgggg cgtgcatcac gtacccgtcg
     1741 aggacgggga cggcggattc ctgggcgtcg taacggacat cgacctgctc gccgcggagc
     1801 agcaccgcgc gttcgggctc aagaaggaca tcgagctcgc ccacgatcgc gagtcggcga
     1861 tcgaggcgtt ccggtcgctg cccgaggtgg cgacccgtct cgtcgaggcg agcgtggagc
     1921 cgctggagat cgcgcacatc gtcgcggtca cgatcgacgc gctcaccgtt cgcctcctcg
     1981 agattggctg ggcggagatc ggtgatccgc cgtgcccgtg gacgtggctc gcgctcggca
     2041 gcgaggcccg gcaggagcag ggattgttca ccgaccagga caacgcgctc atcttcgacc
     2101 cggtcgccat cggcgaggag gaggcggacg cgtacttcgg ccagctcgga gcgatcgtca
     2161 acgatgggct cgaggaggcg gggatcccca ggtgccgggc cggtgtcctc gccggcaacc
     2221 gcgattggcg cggcaccgtg cggacgtggc agaaccgttt ccgtggttgg atcgaggacc
     2281 ccggtcggac cggcagcgcg ttcaccggca tcgcgatcga ttaccggccg gtcaccggac
     2341 cgctggacgc gcggttgcat ctagacgaga tcatccgctc cgcgagagag cagcatgatt
     2401 tcatacgtca cctgggcgcc catgccgtcg cgtcacgccc accgaagggg ctgctcaagg
     2461 atggcgtcgt gcaggggaag aggaggacgg gacggctcga catcaaggcc gttgggatcg
     2521 ggctgatcac caacgtcgcc cggctcctgg ccgtcatgtc gggcctgacc gagaaccgga
     2581 cgacgctccg cttgcgcgac acgacggccc tgggatggtt gaccgaggac gagagccagg
     2641 ggctcgttga ggcgttcgag ctcatgtggc aggttcggct cgagcaccag agccgctgcg
     2701 tcgagcgcgg cgtcgccccg gacgatctcg tcgacccccg agagctgggc ccgctcacgc
     2761 gccaggccct caaggaggcg ttccgcatga tcgacggggc gcaggaccgt ctggccgcgc
     2821 ttctcggcct tcggcgatga gcggcgcccg tcccgcgccg ccgccccgtc gggatcgtcg
     2881 actcccgtgg cgacggctcg agctcgcctc gctcgacttc gaggccacgg gcctcgactt
     2941 cgcaagggac acgatcgttt cgttcggcgt cgttccgatc cgcgcggggc ggatcgacgt
     3001 gggcgatgcc gtctacgagc tcgtcgatcc gggtgaggtc gtgccgtcgc ccgcgtccgt
     3061 gacggtgcat gggctgcggc cggtggacct gcgtggcgcg aggggtctgg acgccgcgcg
     3121 cgccgccctt gccccggtca tcggcgggcg cttcctcgtc acgtgggcgc gcagagtgga
     3181 ggccgggttc ctcgacgtta tgtacggggg aggcgtgcgg tcgtggctcc gacgatgtat
     3241 cgacgcccga gagctgatgt tcgccttgga cgacgaccct cccgtcccgc tcacgttgac
     3301 ctcggtggcc gagcgcctcg gcgtgccggt cgccagcccg caccacgcga tggacgacgc
     3361 cctggtgacg gcgcaggtgt tcctcgtcgc cggcgcccgg ctggaggcgt cgggtgtcag
     3421 gacggtgcgg gcgctgcaaa ggacgaggcg gcccgagagc cccgcgctac gccggcctcg
     3481 cgcgccgatc tgacgacgac gcgatccgca ccgcgtggac ggggacgcgg cagcggcggc
     3541 cgatctcgtg cgccacgtcc ccttccaaga gcgtgcgcca acgcggccgt tcgggatccg
     3601 cgacgacgat cgcgtgaaag ttcctcgcga tcgcccactt ggcgatcatc ttcggggcgt
     3661 tccgcgacct cgaaagcccg acccgcacct cgaagccgcg gctccgcagc tcgtccgcgg
     3721 cctcctccgt gccgtccttc aggacttgcc attcgatccg gttcggctgc aggccggggt
     3781 tcgggagccc gagcgaggtc ccgtagacct tggcgatgcc gagcacggtg atcttggcat
     3841 gcgcgggtgt ggcgagttcg atcgcgcggg ccagcacctc gcgtgggaac ggctgggaga
     3901 cggtcgcgat caggatgcgc tcgggcgggt cgggcatgcg ccggggggcc gcgaccacgg
     3961 ccccccggcc gagcctcgct ctgcgaggtg cctcagacat cctggctgac ggagacgcgc
     4021 ctgaaatcgg tggtgccgag cctccgatcc tccgcccttc tccaccagta cagcgggaag
     4081 tacaggagca cgatgatcac cccgatgatg aacatcttcc cgccgccgag ctgacacccc
     4141 tcgatcgcgc acgccgggat ccccaccgac cagacgacga agtacagcac ggcgagcgcg
     4201 atcgcgccgt acttcatcca ctcggggagc ctgaacgggc ggaagatctc gggccgccac
     4261 cttcgcagga agtagtaccc gaacaggacg gggacgaacg atccgacgta cccgacgttc
     4321 gagagcacgt agatcgaccc gggcgccccg acgaagatca gcaggatgtt cagcacggtg
     4381 ttgagcccca tcgagaagtc cggcacgttg tgccggttga cacgaccgaa gatccgcggg
     4441 ctctggccgt cgagcgccat catgtagagc gacctcgcgc aacccatgat ggcgttcagg
     4501 ctggacaacg cgagcgcgat gatcagcgcc gacgtgacga tcttgctgaa gcccgaaccc
     4561 agggatctga cggccgtctc gacgaggatg gcgttcggat ccaggctctc ctggatgaac
     4621 ttcaggccga gcacgccgag caccgccatc ggcaggaacg tgtagatgaa cgtcccatag
     4681 cctcccgaca ggttcatggc gatcggcgcg tctcggtcag ggttgcggca ctcgccgatg
     4741 tagcacgcgg cggcctccat cgcgatcgcg ttccaggtgt acagcgccga gaactgcaag
     4801 aacacgtaga acgcggtccc ggagaagaat gcggtcccgt ccgggaggtt cgccggccac
     4861 aggttgcttc cggtgacgtc gcccgtgaag aacggcgcga ccgcgatgag cgtcaggggg
     4921 atcatcgaca ggagaccgag cacggtcgcc gcggcggtcc cgaagcggat gccgaggtag
     4981 gcgatgacga acaacaagac cgaaagcacc gcgccgatca cgatcgtgaa gttcgtgacg
     5041 gggaggctgg cgtcgaacag cgtgtgcgtc tgagcccacc accccgacag ctcgaacccg
     5101 aacagaaccg ggaggtacgc gccggtcagg atcatgttca ccgccatgac cggcatccac
     5161 ccgagccagt acatccagac ggtcaccccg ttgatgtgcg ggtacgtcct cggcaaccgg
     5221 tccttgaagg cgtagtaggc gtatgcgggc gcccctcccg tccggtccgg cagcatcgcg
     5281 gccatctcgg ccaggtacag acacagcagc cagcccgtga gggcccagaa gatgaagttc
     5341 gggatggccg ccgccccgag cggtgcgagc accgccggcg tcactcccgt gaccaggatc
     5401 gtgccggcca ggcccaccac gaaggcgccc caccagttgg tgtccttgga gagctcgccc
     5461 ttgacgtagt cctcgacccg cacgaactgt tgctcgtcct gcttcgccat cgcatccccc
     5521 cctcgtcaaa atggccggaa cggcgccggt gggccgggcg ccgccgaacg cggcgaccgg
     5581 gcgggcgggg ccggtcccga gcggccggtt cagaacaggg acaggtactt cttcacctcc
     5641 cagtcggaca ccccctcgtg atactccttc cactcgcgtt ccttcacgtc gcagtagtag
     5701 tcgaggtagt cgccgccccg gaccttgccg agcccctccc ggagcacgtc gtcctggcgg
     5761 aggttgcgga tcgcgtcgag caggtttccg ggcaggagct cgacgccccg acgtcgcagt
     5821 tcctccacgg gcgtttcgta catgttgccc tcgttgggat cgccaggatc cagcttgttg
     5881 tcgatcccgt cgaggcccgc ggccaggatc gccgaggcgg ccaggtacgg gttggcggcg
     5941 ccgtcgatcg tgcggtcctc gaaccgcccc ggcgcgggtg tccggatcat ctgcgtccgg
     6001 ttgttccggc cgtacgcgac gtaggcgggt gcccacgtcg ccccggaggt cggcgcgcca
     6061 acgatgagcc gcttgtagga gttgaccgtc gacgcggtga ccccgatgta cgccttcgca
     6121 tgggccagca ggccgccgag gaagtgatac ccgagctccg agatgccgag gccacgcgcg
     6181 tcgttctcgt cctcgaacag gttcgtgtcc gtgtccggat cccacaggct catgtggaag
     6241 tggagcccgt tcccggtcag gtgcccgaac ggcttgggca tgaaggtcgc ctggaggcca
     6301 cgctgttgag cgagcatgtg caccatgtac cggaagaaga tgagccggtc ggagctcgtg
     6361 acggcgtcgt cgaagacgaa attcgactcg aactgaccgt tcgcgtcctc gtggtcgttc
     6421 gcgtagttgt cccagccgag ctgggtctcg tatttggaca gggtcgtgat gaagtcgtac
     6481 tggcggctga gcgccttcat gtcgtagcag ggccgttcga gggtgtcgag cgggtccgcc
     6541 acctcgatcc tgccgtcctc ggtgagtcgg gtgaggaaga actccgcctc gatgccgatc
     6601 ttgaagacgt acccgcgctc gcgcgccttc gccatgctgc gcttcaagat cgtgcggggg
     6661 cagtagggcc acggctcgcc ctcgacgaac acgtcgcacg cgaaccgcgc gaggttgggc
     6721 tgccacggca ccggcgtgaa gctcgccggg tcgggcatcg ccgcgatgtc gggatcatgc
     6781 ggtccctggc ccagctcgcc ggtcgcgaac cccgcgaacc cggccccttc ggcgaacacg
     6841 tcctcgagat gcgcggcggg gacgagcttg gcgttgggct tgccgtacat ctccacgaac
     6901 tgcgcgaaga cgaactctat gcccatgtcg tcgacgagct tgcggacctg ctcaacggaa
     6961 cccgggcgct cagccatccc tcccccttcc tgctcgcggc tccgcacgaa cgagcgatga
     7021 tcgcatcacg acgttcgcga ggaggtgacc ggccggatgc gtgtcgaggg cggatagacg
     7081 tgctcgaacg aacgtccgag ccacgttcgg gtgatcgaac aacccggatc atccgggatc
     7141 gacatggcgt tggcggacgc tagcggctcg tgccggggcg agtcaaatca gcggttgtgt
     7201 ccgcccgcac ctctcacgcc ccagcctgga gcggttcagc gagcgacgat caccgggcag
     7261 tgggccaggc tgatcacctt gtgcgcgacg gagcccagca ggagcgccga ccaatcggac
     7321 aatccgcgcg acccaagcac gatcccgtcc acctcggccg ccgcggcccc ctcgacgatc
     7381 tcgcgcccgg cgcggcccag cggcgcgcta cgggcctcgc ccttgacgac gacgccctgc
     7441 tcctgaaggg tcgacacgac ccggttcacg agctcgaggg cctcctcggg cggctccatc
     7501 tggccgctga accggccgat ctcgatctcg cgcacgtgga ataccagcac ctcggagccg
     7561 aggttcttgg ccagcccggc ggcgatgccc acggccttct cggagcccgg ggagccgtcg
     7621 accgcgagaa gcagcttctc gaacatgcct ccacctcctc gcctcccctg tacccagacg
     7681 ctcgggcgtt cacgcgcaag gggagggatc gccgaccacc ccctcggtgg cgactcgaac
     7741 gggcgatgac ctaagattcc gtccgccggt ggccgagatc gggccgcctt caccagaggg
     7801 gaggcgtcgg tgacgaccga catcgaagcg ttcatcggcc aggagggccg ggacaaggcc
     7861 atccaggagg tacgcaagca gatcgacgca gccggcgtcg agtacatgta ctaccagttc
     7921 gtctccgtga ccggtcgcat catgggcaag ggcatcccgg ccgcccactg ggagaacatc
     7981 tccaacaagg gcttccagct cgtctacggg tcgacggcga acctgttcgt cgaccgtcag
     8041 ggcaactaca tcggctacgg ccccgaggcc cgcgagctcg tcggcatccc cgagcccgag
     8101 accttctgcg tcttcccctg ggacaacaag accgctcggg tcttctgcac gctgttccga
     8161 ggccgagagg aggaggtcga cggcggagcg ttcctgacct cggattgccg agggaacctc
     8221 aagcgcctgc atacggagtt cgagcaggag accgggctcc acctgcgcgc cgggtgcgag
     8281 cccgagatga tgtggctgaa ggtcgacgcc gacgggaagc cgaccgtcga gggcaagacg
     8341 aagccgtact gctaccacat cgatcagttc tccgagctcc aacccctgat ccacaaggtg
     8401 atggagtacg gccgcgcgat gggcctggac atgatccagg gcgaccacga ggacgcgccg
     8461 ggtcagctcg agctcaattt catgttcgac cgcgcggagg ccacggccga ccgactcacg
     8521 acctaccgcc agatctgccg gcaggtcggg cgcgagatgg gggcgttcgc atgcttcatg
     8581 cccaagccgt tcatgggcgt gtctgccaac ggatgccacc acaacatctc gctgtggcaa
     8641 ggcgacgaga acatgttcat gcccgagggt gacaaccctc ggatgcccgg caagatcggt
     8701 ctgcactcga tcggcggcgt gctggagcat ctgtccgcgc tcaccgcgat cacggcgccc
     8761 acggtgaact cctaccggcg gttctgggac gcggggttct gggcgcccgt gttcgccgac
     8821 tgggggttcc agaaccgcac ggcggccctc cgcatctcgg cgcccggccg gttcgaatac
     8881 cgctcggtgg actcctcggt gaacccgtac ctatcgttcg cggcgctgct cgcggcgatg
     8941 aaggacggga tcgaccgtag cctcgacccg ggcgagcccg aggagcgcaa catctacgac
     9001 gcgatcgcgg gcggcaagga cgtgaagaag gtccctcacg acctcggcgc cgcgctcgac
     9061 gcgctccggg cggacccggt gatccagggc gcgctgccca acgagatgta cagggtcttc
     9121 atgcactata agcaggacga gtgggaccgg ttccgcgcga cggtcagcga gtgggacatc
     9181 gagacctacc tggacgtact accctaggat aaggagctca gcccgtatgt gcggtatcgc
     9241 aggagtcatc catcgagacg gctcggccga catcgggtcc gagatgacgt ccatgctcca
     9301 atccatgaag caccgaggtc cggactcgag cggctatgcg ttgttcggtc c
//
LOCUS       DATBRK010000246         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1338184, whole genome shotgun sequence.
ACCESSION   DATBRK010000246 DATBRK010000000
VERSION     DATBRK010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338184"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..483
                     /locus_tag="VIH70_07460"
     CDS             112..483
                     /locus_tag="VIH70_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116234.1"
                     /translation="MTEIHRYVGFLVVAIFTIGWLFGLVLWISRREAGDWFWRWLVAA
                     QTVAIAQALIGVILLLAGRRPTTALHYVYGFGPLVIFAIGHLLAREEAFRAKPWAPFA
                     LASFISFGLSLRALTTGLGIG"
     gene            525..2453
                     /locus_tag="VIH70_07465"
     CDS             525..2453
                     /locus_tag="VIH70_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116235.1"
                     /translation="MQARYSRWDDTQDPFGPELSAGELLEELSDDALSGLGIEGALSR
                     LMRRGMRGSFDGLDALRARLRERRAREQGNLDLAGPLEELRDRLDDIVDRERSTLSFR
                     AEDDARMREAFLDALPPDVPGRIGELSDYRFADPEAQAAFDELLEHIREQVMGAYFRS
                     MAEGLQSMSPDDLARFRDMLADLNRMIEQRERGEPYDFEGFMQRHGDIFPGNPKTLDE
                     LLEQMAARMAAMSRLLASLSPEERAELRALAEQVLQDMDLAFEVDRLGSNLASALPDL
                     GWGEPTLAGGDDPLPLSATIDAMERLHDLEELDRSLRGDYPGASIDDVDEDALRQALG
                     EDAIRDLRRLKEIERALERAGLVARERGRLEVTPKGARSMGERALVRVFEELRRDREG
                     VHEARETGGLAEPTGATRPWRFGDAGQIAVQRSVFNAVVRGGPGARVRLEPDDLELLE
                     AEQRTESATALLLDLSFSMPLRGHDVHAKRMALALHALIEGRYPHDTLYLIGFSDYAR
                     RMQPTELAAAGWERVYGTNMHHAFHLAGRLLAQHPRATRQVIMVTDGEPTAHLEGERA
                     RFAWPPMPKTIRLTLAEATRLSRSGVTLNVFMLEDSPGLIAFMERLAELTAGRVFLMD
                     DRELGSFVLRDFVRRRAS"
     gene            complement(2473..3294)
                     /locus_tag="VIH70_07470"
     CDS             complement(2473..3294)
                     /locus_tag="VIH70_07470"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8116236.1"
                     /translation="MNALERWRREQAGWAIPDEILAAAPEPPWGFPPELFRSRAERAP
                     AEPNATTRIALEALGTGGEVLDVGVGGGATSLPLAAVANRITGVDESAEMVAEFLASA
                     REAGVRADAVEGRWPDASPDTPTAEVVVCGHVLYNVGELEPFVRALDDHARRRVVVEI
                     TSTHPLVWMADLWRRFHGLERPTGPSADLAFAAIAELGVEVRREDESRVPRSGGFERR
                     DDAIAFIRRRLCLSADRDADLAEALGERLVQRDGLWAAGPLEHPIVTMWWDRPAT"
     gene            complement(3291..>4232)
                     /locus_tag="VIH70_07475"
     CDS             complement(3291..>4232)
                     /locus_tag="VIH70_07475"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8116237.1"
                     /translation="NKRSVTLDLGTDDGRSAARRLALASDVVVENFRPGLMERFGLDH
                     ASLSAEHPGLVYCSLTAFGESDESARPGYDIIVQALSGLMSFTGHPGGEPTKVGVALL
                     DVICGLYACNAIQAALLGRNATGRGSRVSVSLFDASIAALVNQAANYLLGGLVPQPLG
                     NAHPNIVPYQLVEAADRPFILAAGNDRLFERTCSVIGRPELASDERFATNADRVRNRD
                     QLIPILSQALARRPAAEWLTALEAASVPCAPVRTLDEVFASPEGAASIQTIDDPERGS
                     LRLVADPIRVDGRVPPARMPPPRLGEHSDDVRSELEG"
BASE COUNT          584 a         1488 c         1569 g          591 t
ORIGIN      
        1 cgccggctca acaaggacct ggaggcgcgg gcgggcggca gccggtaccg gtcggccgaa
       61 cccgacgcgt aacgacgccc ggcggctcgc agcccgtagg attcggccgc cgtgaccgag
      121 atccaccgct acgtcggttt cctcgtcgtt gcgatcttta cgatcgggtg gctcttcgga
      181 ctggtccttt ggatctcgcg cagggaggcg ggggattggt tctggcgatg gctggtcgcg
      241 gcgcagaccg tggccatcgc gcaggcgctg atcggcgtga tcctgctgct cgccggccgt
      301 cgcccgacca cggcactgca ctacgtctac gggttcgggc cgctcgtgat cttcgcgatc
      361 ggccatctcc tggcgcggga ggaggcgttc cgcgccaagc cgtgggcgcc gttcgccctg
      421 gcctcgttca tctctttcgg actgtccttg cgagcactca ccaccgggct cgggatcggc
      481 taagcggaat gaacgttccc cacgcctcgg cgtaggctgg tcgggtgcag gcccgctact
      541 cgcgctggga cgacacccag gatccgttcg gccccgagct gtccgcgggc gagctgctcg
      601 aggagttgtc cgacgacgca ctgtcgggtc tggggatcga gggcgcgctc tcgcggctga
      661 tgcgccgcgg gatgcggggg agcttcgacg ggctggacgc actccgcgcg cggctccgcg
      721 aacggcgcgc ccgggagcag gggaacctcg acctcgccgg cccactcgag gagctccgcg
      781 atcggctcga cgacatcgtc gatcgtgagc gctcgacgct ctcgttcagg gccgaggacg
      841 acgcgcggat gcgcgaggcg ttcctcgacg cgctcccgcc cgacgtcccg ggccggatcg
      901 gggagctgag cgactaccgg ttcgccgacc ccgaggcgca ggcggccttc gacgaactgt
      961 tggagcacat ccgcgagcag gtgatgggcg cgtacttccg cagcatggcc gaggggctgc
     1021 agtcgatgtc gccggacgac ctcgcgcgct tccgcgacat gctcgccgat ctcaaccgga
     1081 tgatcgaaca gcgcgagcgg ggcgagccct acgacttcga ggggttcatg cagcgccacg
     1141 gcgacatctt ccccggcaac ccgaagacgc tggacgagct gctcgagcag atggccgccc
     1201 ggatggccgc gatgtcccgg ctgctcgcgt ccctttcgcc ggaggaacgc gccgagctcc
     1261 gggcgctcgc cgagcaggtc ctgcaggaca tggatctggc gttcgaggtc gaccggctcg
     1321 ggtcgaacct ggcctccgcc cttccggacc tggggtgggg agagccgacg ctcgccggcg
     1381 gcgacgaccc gctgccgctc tcggcgacga tcgacgcgat ggaacggctg cacgacctcg
     1441 aggagctcga ccgctcgctg cggggggact acccgggcgc ctcgatcgac gacgtggacg
     1501 aggacgccct gcgccaggcg ctcggcgagg acgccatccg cgacctgcgg cggctcaagg
     1561 agatcgagcg cgcgctggag cgcgccggac tcgtcgcgcg cgagcgggga cggctcgagg
     1621 tcaccccgaa gggggcgcgc tcgatggggg agcgggcgct ggtccgggta ttcgaggagc
     1681 tccggcggga ccgggaggga gtgcacgagg cccgcgagac cggcggcctc gccgagccca
     1741 ccggcgcgac ccggccgtgg cgtttcggcg acgccggtca gatcgcggtc cagcggagcg
     1801 tgttcaacgc cgtggtccga ggcggccccg gagcccgggt gcgactcgaa cccgacgacc
     1861 tggagctgct cgaggccgag cagcgcaccg agtcggcgac cgcgctgctg ctggacctct
     1921 cgttctcgat gccactccgc ggccacgacg tccacgccaa gcggatggcc ctcgccctgc
     1981 acgcgctgat cgaggggcgg tacccgcacg acaccttgta tctgatcggc ttcagcgact
     2041 acgcgcgccg gatgcaaccg acggagcttg cggccgcggg ctgggagcgc gtgtacggga
     2101 ccaatatgca tcacgcgttc cacctcgccg gccggctgct cgcgcagcat ccgcgcgcga
     2161 cgcggcaggt gatcatggtg accgacggcg agccgaccgc gcacctcgag ggggagcgtg
     2221 cgcgcttcgc gtggccgccg atgccaaaga cgatccgact gacgctcgcc gaggcgacca
     2281 ggctctcgag atcgggcgtg accctcaacg tgttcatgct cgaggactcg ccggggctga
     2341 tcgcgttcat ggaacggctt gccgagctca cggcggggcg tgtcttcctg atggacgacc
     2401 gggagctcgg gtcgttcgtg ctccgggact tcgtccgccg gcgagcgtcc tagcgcaccg
     2461 gcgggccgcc cgtcaggtcg cggggcggtc ccaccacatc gtgacgatcg ggtgctcgag
     2521 gggccccgcg gcccagagcc cgtcgcgttg gaccagccgc tcgccgagcg cctccgcgag
     2581 gtcggcgtcg cgatccgccg agaggcagag gcggcgtcgg atgaacgcga tcgcgtcgtc
     2641 ccggcgttcg aagccgccgg agcgagggac gcgcgactcg tcctcgcgac ggacctcgac
     2701 gccgagctcg gcgatcgccg cgaacgcgag gtccgcgctc gggcctgtcg gccgttcgag
     2761 accgtggaag cgccgccaca ggtccgccat ccacacgagc gggtgcgtcg acgtgatctc
     2821 gacgacgacg cggcggcggg cgtggtcgtc gagcgctcgc acgaacggct cgagctcgcc
     2881 gacgttgtac aggacgtgac cgcacaccac gacctccgcc gtaggcgtat cggggctcgc
     2941 gtccggccac cggccctcga cggcgtccgc ccggacgccc gcctcgcggg cggacgcgag
     3001 aaattcggcc accatctcgg ccgactcgtc gaccccggtg atccggttcg cgaccgccgc
     3061 gagcggcagg gacgtcgctc cacctccgac ccccacgtcc aacacctcgc cccccgtccc
     3121 gagggcctcg agcgcgatcc gggtcgtcgc gttgggttcg gcgggagccc gttcggcccg
     3181 gctccggaac agctccggcg ggaagcccca gggcggctcg ggcgccgcgg ccaggatctc
     3241 gtcggggatc gcccagccgg cttgctcgcg acgccagcgt tcgagcgcgt tcacccctcg
     3301 agctccgacc gcacgtcgtc cgagtgctca ccgagccggg gcgggggcat ccgggcgggc
     3361 gggacgcgac cgtcgactcg gatcggatcg gcgaccagcc ggagcgatcc tcgttcgggg
     3421 tcgtcgatcg tttggatcga ggccgcgccc tcgggggagg cgaagacctc gtccagcgtt
     3481 cggacgggcg cgcaggggac cgacgcggcc tcgagcgccg tgagccactc ggccgcgggg
     3541 cggcgcgcga gcgcctgcga caggatcggg atgagctggt cgcggttgcg aacgcggtcc
     3601 gcgttggtcg cgaaccgctc gtcggatgcg agctcgggcc gcccgatcac gctgcaggtc
     3661 cgctcgaaaa gccggtcgtt ccccgcggcc aggatgaacg gccggtcggc ggcctcgacc
     3721 agctggtagg gcacgatgtt gggatgcgcg ttgccgagcg gctgcggcac gagaccgccg
     3781 agcaggtagt tcgcggcctg attgacgagc gcggcgatgc tcgcgtcgaa cagtgacacg
     3841 ctcacgcgcg acccgcgtcc ggtcgcgttc cgcccgagga gcgccgcctg gatcgcgttg
     3901 cacgcgtaga ggccgcagat aacgtcgagg agcgcgaccc cgaccttcgt gggttcgccg
     3961 cccgggtgcc cggtgaagct catcaggccc gacagggcct ggacgatgat gtcgtagccc
     4021 gggcgcgcgc tctcgtcgct ctcgccgaac gccgtgagcg agcagtagac gaggccgggg
     4081 tgctccgccg acagcgaggc gtgatcgagc ccgaagcgct ccatcaggcc ggggcggaag
     4141 ttctcgacga cgacgtccga ggcgagcgcg aggcggcggg cggccgaccg cccgtcatct
     4201 gtgccgaggt cgagcgtgac cgagcgcttg tt
//
LOCUS       DATBRK010000247         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2204041, whole genome shotgun sequence.
ACCESSION   DATBRK010000247 DATBRK010000000
VERSION     DATBRK010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204041"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VIH70_07480"
     CDS             complement(<1..970)
                     /locus_tag="VIH70_07480"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HEY8116238.1"
                     /translation="MCGIVGYVGPDQALPVVLEGLRRLEYRGYDSAGVAMIDGGLSVV
                     KRAGKLSELDAALEVVGAVPGSIGMGHTRWATHGAPTDRNAHPHLDCDGRVAVIHNGI
                     IENFTDLRTGLQKRGHVLESDTDTEVLAHLIEEKEGSLADRVRATAAELDGAYALVVL
                     ALDEPDVIVGVRVSSPLIVGLGDGENVLASDIPAVLQKTRIVLPIEENQVVEVRAGGV
                     RVTDLHGDEVEVEPFEVAWDVARAQKGGFDDFMLKEIHEQPAAIRDTLVGRVSEQGLL
                     ALDELHTDTDVLREVDKVFVVACGTAFHSGLVAKYAIEHWTRLPVEI"
     gene            complement(1023..2351)
                     /gene="glmM"
                     /locus_tag="VIH70_07485"
     CDS             complement(1023..2351)
                     /gene="glmM"
                     /locus_tag="VIH70_07485"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY8116239.1"
                     /translation="MGRLFGTDGVRGVFGRDLTTDLARSIGRAAVTVIARDRHEPPTF
                     LIGRDTRASGPVLEDALVDGIVAGGGNAIRAGVVPTPGVAYLTTELGAESGVVISASH
                     NPPEDNGIKFFGFRGFKLSDRLEDEIEAEIAAGGGEGGSAGRELPMPSGGSSYLDHVV
                     ASAQSRLDGWRVVVDCANGAASAFAPAALERLGAEVISIHAKPDGSNINVDCGALYPE
                     VVAEAVVTHGAHAGVAHDGDADRALFADAAGNVIDGDQVLAACAISMQQDGTLTGDTV
                     VATVMSNIGFHRAMHDHGIDVISAPVGDRYVLEQMLQHEVALGGEQSGHLIFRDQATT
                     GDGLITAVRFLSLAARRGVPVGELAAVMRRFPQVLVNVEVEHKERLGDAREVWDTVRA
                     NEEALDGSGRVLVRASGTEPIVRVMVEAESEELARRHADSVAERIASALA"
     gene            complement(2392..3129)
                     /gene="arsM"
                     /locus_tag="VIH70_07490"
     CDS             complement(2392..3129)
                     /gene="arsM"
                     /locus_tag="VIH70_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007380029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEY8116240.1"
                     /translation="MSEVREQVREHYREVAIDVRDGGDGCCDSGCCGSKDRVWGAELY
                     EAVERAELPDAAVLASLGCGNPIAVAELREGETVLDLGSGGGIDVLLSARRVGSTGHA
                     YGLDMTDEMLDLARRNAADAGAENVEFLKGYIEDVPLPDASVDVIISNCVINLSADKH
                     AVFEEMRRVLRPGGRIGISDVVASDDLSPAARAERGSLVGCIAGALSFGEYRSGLEGA
                     GFGEIEIEPTHDVADGMHAAIVRARRT"
     gene            complement(3126..3482)
                     /locus_tag="VIH70_07495"
     CDS             complement(3126..3482)
                     /locus_tag="VIH70_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8116241.1"
                     /translation="MRELQLLEGAPAACCSSLGATAMSDQDAEATAAVFKALSDPARV
                     RIVNLLANAEEPVCVCDLTPKLRLSQPTVSFHLKKLVQAGLLEREQRGVWAFYTVNRE
                     VLPRLGRIFELEGAIA"
     gene            3587..3799
                     /locus_tag="VIH70_07500"
     CDS             3587..3799
                     /locus_tag="VIH70_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY8116242.1"
                     /translation="MPDLVKVLSASSIMEGEIVKARLEGEGIPVLLKGGGSDPYGMGV
                     AYVFVPSEFEAQARLVLEDVSDETSG"
     gene            complement(3830..>3892)
                     /gene="rpsI"
                     /locus_tag="VIH70_07505"
     CDS             complement(3830..>3892)
                     /gene="rpsI"
                     /locus_tag="VIH70_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY8116243.1"
                     /translation="KERRKYGLKKARKAPQYSKR"
BASE COUNT          584 a         1426 c         1304 g          578 t
ORIGIN      
        1 cgatctcgac cggtaggcgc gtccagtgct cgatcgcata cttggcgacc agccccgaat
       61 ggaacgcggt gccgcaggcg acgacgaaca ccttgtcgac ctcgcgcagg acgtccgtgt
      121 cggtgtgcag ctcgtccagc gcgagcagac cctgctcgct gacgcggccg accagcgtgt
      181 cccggatcgc cgcgggctgc tcgtggatct ccttcagcat gaagtcgtcg aacccgccct
      241 tctgcgcgcg cgcgacgtcc cacgccacct cgaagggctc gacctcgacc tcgtcgccgt
      301 gcagatccgt gacgcgaacg ccgccggctc gcacctcgac cacctggttc tcctcgatcg
      361 gcaggacgat ccgcgtcttc tggagcaccg cggggatgtc ggacgcgagg acgttctccc
      421 catcgccgag cccgacgatc agcggcgacg agacccggac gccgacgatg acgtcgggtt
      481 cgtcgagcgc gaggacgacg agcgcgtacg ccccgtcgag ctcggcggcc gtggctcgca
      541 cgcgatccgc gagcgaaccc tccttctcct cgatcaggtg ggcgaggacc tccgtgtcgg
      601 tatcggactc caggacgtgg ccgcgcttct ggagaccggt acgcaggtcc gtgaagttct
      661 cgatgatccc gttgtggatc accgcgacgc gcccatcgca gtccaggtga gggtgcgcgt
      721 tccggtcggt cggcgcgccg tgggtcgccc accgcgtgtg ccccatcccg atcgagcccg
      781 ggacggcgcc gacgacctcg agggcggcgt cgagttcgga cagcttgccg gctcgcttca
      841 ccaccgagag gccaccgtcg atcatcgcga cgccggccga gtcgtacccg cggtactcga
      901 gacgtcgcag gccctcgagg acgacgggga gcgcctggtc ggggcctacg tagcccacga
      961 tgccgcacat gaggttcgag ggtaacagga gcgccgcgtt cgtcgaaagg tggagaaggc
     1021 ccctacgcga gggcggacgc gatgcgttcg gcgaccgaat ccgcgtgcct gcgggcgagc
     1081 tcctcgctct cggcctcgac catgacgcgg acgatcggct cggtgcccga ggcccggacg
     1141 agcactcgcc cggagccatc gagggcctcc tcgttcgcgc ggaccgtgtc ccagacctcc
     1201 cgggcatccc cgagccgctc cttgtgctcg acctcgacgt tcacgagtac ctgggggaag
     1261 cggcgcatca ccgccgcgag ctcgccgacg gggacccccc gtcgggccgc gagcgagagg
     1321 aatcgcaccg cggtgatcag cccgtccccc gtcgtggcct ggtcccggaa gatcaggtgt
     1381 cccgactgct cgccgccgag cgccacctcg tgctgcagca tctgttcgag cacgtaccgg
     1441 tcgccgaccg gtgccgagat cacgtcgatg ccgtggtcgt gcatcgcccg gtggaacccg
     1501 atgttcgaca tcacggtcgc gacgaccgtg tcgccggtca gcgtgccgtc ctgctgcatc
     1561 gagatcgcgc aggccgcgag cacctgatcg ccgtcgatca cgttcccggc ggcgtcggcg
     1621 aacagcgcgc ggtcggcgtc gccgtcgtgc gcgaccccgg catgggcgcc gtgcgtcacg
     1681 accgcctccg cgaccacctc ggggtagagc gcgccgcaat cgacgttgat gttcgagccg
     1741 tcaggcttgg cgtggatcga gatcacctcg gcgcccaggc gttcgagcgc ggcgggcgcg
     1801 aacgcggagg ccgcgccgtt cgcgcagtcg acgaccaccc gccacccgtc gaggcgcgac
     1861 tgcgcggaag ccacgacgtg atcgaggtag ctcgagccgc cggacggcat cgggagctcg
     1921 cgcccggccg agccgccctc gccgcccccg gccgcgatct ccgcctcgat ctcgtcctcg
     1981 aggcgatccg acagcttgaa cccgcggaac ccgaagaact tgatcccgtt gtcctcgggt
     2041 gggttgtgcg aggccgagat caccacgccg gactcggcgc cgagctcggt cgtcaggtac
     2101 gccacccccg gcgtgggaac gacgccggca cggatcgcgt tgcccccgcc ggcgacgatg
     2161 ccgtccacga gcgcgtcctc caggaccggg ccggatgcgc gggtgtcgcg gccgatcaag
     2221 aacgtcggcg gttcgtggcg atcgcgggcg atcaccgtga cggccgcgcg tccgatcgaa
     2281 cgcgcgagat ccgtggtcag gtcgcgtccg aacacgcctc gaacgccgtc cgtgccgaag
     2341 agccgcccca tcgcgcgcga aggctacccc aaaccctccg ccgtcatccg gtcaggttcg
     2401 gcgcgcccgg acgatcgccg cgtgcatgcc gtccgcgacg tcgtgggtgg gctcgatctc
     2461 gatctcgccg aaccccgcac cctccagacc cgagcggtac tcgccgaacg acagagcgcc
     2521 ggcgatgcat ccgacgaggc tcccgcgctc ggcccgcgcc gcgggcgaga ggtcatcgga
     2581 cgctacgacg tcgctgatcc cgatacggcc gcccggccgg agcacgcgcc gcatctcctc
     2641 gaacaccgcg tgcttgtcgg cggacaggtt gatcacgcag ttcgagatga tcacgtccac
     2701 gctcgcatcc ggcagcggga cgtcctcgat gtaccccttg aggaactcga cgttctcggc
     2761 gcccgcgtcg gcggcgttgc gccgagcgag atcgagcatc tcgtcggtca tgtcgagacc
     2821 gtacgcgtgc ccggtcgacc cgacccttcg cgccgaaagc agcacgtcga tcccgccgcc
     2881 cgaacccaga tcgagcacgg tctcgccctc gcgcagctcg gcgacggcga tcgggttccc
     2941 gcacccgaga ctcgccagga cggcagcgtc ggggagctcg gcccgctcga ccgcctcgta
     3001 cagctcggcg ccccagaccc ggtccttcga gccgcagcat cccgaatcgc aacacccgtc
     3061 gccgccgtcc cgcacgtcga tcgcgacctc gcggtagtgc tcgcggacct gctccctgac
     3121 ctcgctcatg cgatcgctcc ttccagctcg aagatcctcc cgaggcgcgg gagcacctcg
     3181 cggttcaccg tgtagaacgc ccacacgccg cgctgctcgc gttccagcag accggcctgc
     3241 acgagcttct tcaggtggaa gctcacggtg ggctgcgaca ggcggagctt gggcgtcagg
     3301 tcgcacacgc agaccggttc ctcggcgttg gccaacaggt tcacgatccg cacgcgtgcc
     3361 gggtccgaca gcgccttgaa gacggcggcc gtcgcctcgg cgtcctggtc cgacatcgcg
     3421 gtcgcgccca gcgaggagca acacgcggcc ggcgcgcctt cgagcagctg cagttctcgc
     3481 atcggaaggc cccttcgaga agatcgatgt ctatcgatgg cagcgtagac gagacatcga
     3541 tgaacgtcaa tggctccgac gtggcacggc ctgcgacaat gcgtgcatgc cggacctcgt
     3601 caaggtgctc tccgcctcct cgatcatgga gggcgagatc gtcaaggccc gcctcgaggg
     3661 ggaggggatc cccgtgctgc tcaaaggcgg gggctccgac ccctacggga tgggcgtcgc
     3721 ctacgtcttc gtcccctccg agttcgaggc acaggcgagg ctggtgctcg aagacgtgag
     3781 cgacgagacg tccggctgaa ccccgcgagg tcggccgcga gcgaccggat cagcgcttgg
     3841 agtactgcgg ggccttgcgg gccttcttga ggccgtactt gcgccgctcc tt
//
LOCUS       DATBRK010000248         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1485125, whole genome shotgun sequence.
ACCESSION   DATBRK010000248 DATBRK010000000
VERSION     DATBRK010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485125"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VIH70_07510"
     CDS             <1..189
                     /locus_tag="VIH70_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116244.1"
                     /translation="DSFRRHVELAEARGLPVSIVERRELSFDVDRPDDILTLLADGRR
                     GRTREVCLQMDLGARLRA"
     gene            204..410
                     /locus_tag="VIH70_07515"
     CDS             204..410
                     /locus_tag="VIH70_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018584884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116245.1"
                     /translation="MPQGTIKDFEGQERIGSLLMDDRTEVAIDAVSVEGSGLRSLRLG
                     QRVRFDLVEEAGRKVARNLQIVTL"
     gene            complement(443..748)
                     /locus_tag="VIH70_07520"
     CDS             complement(443..748)
                     /locus_tag="VIH70_07520"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY8116246.1"
                     /translation="MNKSGLIAEVAKRTGGSKADIGRMIDSSMDVIRETVSRGDRVSL
                     VGFGTFERKRRNKRVARNPRKPMTPIVVPARDLPWFTAGKEFREMVASKRRRVPSKR"
     gene            943..1476
                     /locus_tag="VIH70_07525"
     CDS             943..1476
                     /locus_tag="VIH70_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116247.1"
                     /translation="MFALNLPSANDFTVTPPQPAQRGPRFLTGVVSSPPAPSPSPASG
                     AFGGIDAAAQGEPDQTIYRAYAVTDDGFDDLDQMALYYLTGNPIVDVTIDDPTHVDGN
                     PARLIVADIDRGGEVTSSVAAAITLGDGATGYLIVAIFSPGGWEDERDDFERIVRSFQ
                     TDVPPGLETFPVDGPAS"
     gene            1491..1886
                     /locus_tag="VIH70_07530"
     CDS             1491..1886
                     /locus_tag="VIH70_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116248.1"
                     /translation="MDWEPIKRTGKPDPRSVDERTGATRIVLHFPISASPPREWREFL
                     ESRHRGQDYPPVSIQEAEIIVLPLESEEDFITWTENVDRDIQLANEFYVAVLVEAEQE
                     RNVAPEAVEERVRKLEEARTWTKQLEPPA"
BASE COUNT          430 a          865 c          881 g          442 t
ORIGIN      
        1 gactcgttcc gacggcacgt ggagctcgcc gaggcgcgtg gcctcccggt ctccatcgtc
       61 gagcgccgcg agctgtcgtt cgacgtcgat cgccccgacg atatcctcac gctgctcgcc
      121 gacggccggc gagggcggac ccgagaggtc tgcctccaga tggacctcgg cgcgcgcctg
      181 cgcgcgtagc tcgggagggc gcgatgccac agggcacgat caaggacttc gagggccagg
      241 agcggatcgg cagcctgctg atggacgacc gaaccgaggt ggcgatcgac gcagtctcgg
      301 tcgaggggtc gggcctgcgt tcgctgcggc tcggccaacg cgtccggttc gacctggtgg
      361 aggaggccgg acgcaaggtc gcgaggaacc tgcagatcgt gacgctctag cacggcgcca
      421 cgacggcgcg gcgtttcgaa cgctatcgct tggacggaac gcgtcgccgc ttggacgcga
      481 ccatctcgcg gaactccttc ccggcggtga accacggcag atcgcgcgcc ggcaccacga
      541 tcggggtcat cggcttgcgc gggttccgtg cgaccctctt gttcctgcgc ttgcgctcga
      601 aggtcccgaa cccgaccagc gagacccggt caccacgcga caccgtctcc cggatcacgt
      661 ccatggacga atcgatcatg cgaccgatgt ccgccttgct tccgccggtg cgcttcgcca
      721 cttccgcgat cagtccgctc ttgttcacgt ccccaccccc aggatccgct gcggcaccgt
      781 aggcacccga cgggccgctg gtcaatgcct cgttggcgtg caaccatctt gcccatgaga
      841 aggggtccgg cgctactcgt ttgcctgatc gccttccttc catgcgtggc ctgtacccgt
      901 gactccggac gcgaattcgc ccgctattac gacgagcaag ggatgttcgc cctgaacctg
      961 ccctcggcga acgacttcac cgtcaccccg ccacagccgg cccagcgcgg gccccgtttt
     1021 cttaccggcg tggtttcgag cccgcccgcg ccgagcccct cccccgcctc cggagcgttc
     1081 ggcggcatcg acgcggccgc ccagggcgaa ccggaccaga cgatctatcg ggcctacgcc
     1141 gtgacggacg acggcttcga cgacctcgat cagatggcgc tctactacct gaccggcaac
     1201 cccatcgtcg acgtcacgat cgacgatccg acgcatgtgg acggcaaccc cgcgcgactg
     1261 atcgtcgcgg acatcgatcg gggcggcgag gtcacctcga gcgtcgccgc agcgatcact
     1321 ctcggcgacg gagcgacggg ctacctgatc gtcgcgatct tctccccggg tggctgggag
     1381 gacgaacgcg acgacttcga gcggatcgta cgatcgttcc aaacggacgt gccgcccgga
     1441 ctcgagacgt tccccgtgga cggtccggcc tcctgatagc atcggccgcc atggactggg
     1501 agccgatcaa acggacaggg aagccggatc cacgcagcgt cgacgaacga accggagcga
     1561 cccgcatcgt ccttcacttc ccgatctcgg cgtcgccgcc gcgtgagtgg agggagttct
     1621 tggaatccag gcatcgtgga caggactacc cgccggtctc cattcaagag gcggagatca
     1681 tcgtccttcc tctcgaaagc gaagaggact tcatcacgtg gacggagaac gtggacaggg
     1741 atatccagct ggccaacgag ttctacgtgg cggtcctcgt cgaggcggag caggagcgaa
     1801 acgtggcccc agaggcggtg gaggagcggg tgcggaagct cgaggaggca cgcacctgga
     1861 ccaaacagct cgagccacct gcatgagccg tcggcaggat cgcggcgtcc tcgcccgagg
     1921 gtcctcgtga gccagggggc aactccgcgt cgatagtcct ctgggttttg aagtagcccc
     1981 gtcgggggac ctcaccctaa aatgggctcg tttggtagct tcagcggtcg aggctcgact
     2041 ggctgcaacg acctttggat cgacctggag aagcgccgat gagcatatcg tccgacaact
     2101 ctgcgaggag atggccagcc ggacttcggg cgtggggaga cttcaggtgg cagcagttcc
     2161 gttcctggca gatgtatcgg cgggcgtggg gaggctcctg gtatcggttt cgttccgggc
     2221 tgacgcacgg tcgggatcga cagacgggga acggccactg gttggaccgg tttcggtccg
     2281 ggctctcgta cggtagggcc aatacgctcg ccttcgcggc cagcatcatc ctcggctcgg
     2341 cggcgcttgt tggatttctc acgatggaac gagaaagtgc cccggcactg ctgggcgaag
     2401 ccttcacggt cggggtgacg gacgagggag tcccttacgt cgcgttagac cccgaagggt
     2461 tggggcaggc gcaaggtggg gcggcgaacg acggggccag ccgtgggagc ggcccctcgc
     2521 cggttccgct cttttccgta gacacgagcg ggggtgtcct atcgttcgtc ggcggaggag
     2581 gctcgtcggt tgggagcgat ccccagcctc cgcctggt
//
LOCUS       DATBRK010000249         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1338775, whole genome shotgun sequence.
ACCESSION   DATBRK010000249 DATBRK010000000
VERSION     DATBRK010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338775"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VIH70_07535"
     CDS             complement(<1..591)
                     /locus_tag="VIH70_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8116249.1"
                     /translation="MSKQKFERNKPHLNVGTIGHIDHGKTTLTAAISKVLAEQGVSGT
                     EVRDFNSIDNAPEERQRGITINTSHVEYETENRHYAHVDCPGHADYIKNMITGAAQMD
                     GAILVVSAADGPMPQTREHILLARQVEVPSIVVALNKADMVDDPELLELVEIEVRELL
                     SNYEFPGDDIPVVQVSALKALEGDPEWTPKILELMAQ"
     gene            complement(613..>2412)
                     /gene="fusA"
                     /locus_tag="VIH70_07540"
     CDS             complement(613..>2412)
                     /gene="fusA"
                     /locus_tag="VIH70_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8116250.1"
                     /translation="LRVLDGAIAVFDAVAGVEPQSETVWRQADRYRVPRIAFINKMDR
                     TGADFFASVQSMVDRLGARPVPVQLPLGQEEHFRGVIDLVEMRALVWQDELGTNYEVE
                     EIPAELLEQAQEYHHQLIDSVADHDDELLETYLLDEDAVTAEMLKRALRAATLDITVT
                     PVLAGSAFKNKGVQPLLDAVVDYLPSPLDVPPVHGIDPRTEHELSRRPTVDEPFSALA
                     FKVMSDPYVGKLTYIRVYSGQVNAGDRVLNTTTGKTERVGRILQMHANHREEREQIMA
                     GEIAATVGLKNTTTGDTLATDTAPIRLESMDFPEPVISVAIEPKTKGDQDKLGAGLQR
                     LAEEDPTFRVRTDDETGQTLISGMGELHLEIIVDRLTREFSVDANVGRPQVAYRETVS
                     KPVEKIEGRFVRQTGGRGQYGHAVINLEPMDPGDGYEFVDKIVGGKIPKEYISSVDLG
                     IQEAMESGVMSGYPVVDVRVELVDGSFHEVDSSEMAFKIAGSMAFKNAEQRAKPKLLE
                     PVMAVEVVTPEEYLGDVMGDLNSRRGRVEGLEPRGNAQAIRARVPLATMFGYATDLRS
                     TTQGRATFTMQFDRYEEVPQSIAGELVDSPTPS"
BASE COUNT          346 a          797 c          819 g          450 t
ORIGIN      
        1 ctgcgccatc agctcgagga tcttcggagt ccactccgga tccccctcga gcgccttcag
       61 ggccgagacc tgcacgacag ggatgtcgtc gcccgggaac tcgtagttcg agagcagctc
      121 gcgcacctcg atctcgacga gttcgagcag ctcggggtcg tcgaccatgt cggccttgtt
      181 cagggcgacg acgatcgacg gcacctcgac ctgacgggcg agcaggatgt gctcgcgcgt
      241 ctgcggcatc gggccgtccg cggccgacac gaccaggatc gcgccgtcca tctgggcggc
      301 gccggtgatc atgttcttga tgtagtcggc gtggcccggg cagtcgacgt gcgcgtagtg
      361 acggttctcc gtctcgtact cgacgtgcga cgtgttgatc gtgatgccgc gctgccgctc
      421 ctcgggcgcg ttgtcgatcg agttgaagtc ccggacctca gtgcccgaga caccctgctc
      481 ggccagaacc ttcgaaatcg cggcggtcag ggtcgtcttg ccgtggtcga tgtgaccgat
      541 cgtgccgaca ttgagatgcg gcttgttgcg ttcaaacttc tgcttggaca cttctactta
      601 cctccggttg ctttacgagg gggtcggtga gtcgacgagc tcgcctgcga tcgactgagg
      661 gacttcctcg tagcgatcga actgcatcgt gaacgtggcc cgcccctgcg tcgtcgagcg
      721 caggtcggtg gcgtagccga acatggtcgc gagcgggacg cgcgcgcgga tcgcctgcgc
      781 gttgccgcgc ggctcgaggc cctcgacgcg cccccggcgc gagttgagat cgcccatcac
      841 gtcgccgagg tactcctcgg gcgtcacgac ctcgacggcc ataacgggtt cgagaagctt
      901 cggcttcgcc cgctgctcgg cgttcttgaa cgccatcgag ccggcgatct tgaaggccat
      961 ctcactcgag tcgacctcgt ggaacgagcc gtcgacgagc tcgacccgga cgtcgacgac
     1021 gggatagccg gacatgactc ccgactccat cgcctcctga atgccgaggt cgaccgaaga
     1081 gatgtactcc ttgggaatct tgccgccgac gatcttgtcg acgaactcgt agccgtcgcc
     1141 gggatccatc ggctcgaggt tgatcacggc gtggccgtac tggccgcggc cgccggtctg
     1201 gcggacgaag cgcccttcga tcttctcgac cggcttgctg accgtctcgc ggtacgcgac
     1261 ctggggacgg ccgacgttgg cgtcgacgct gaactcgcgc gtgagccggt cgacgatgat
     1321 ctccaggtgg agctcgccca tgcccgagat cagcgtctgg cccgtttcgt cgtccgtgcg
     1381 gacgcggaag gtcgggtcct cctcggcgag gcgctgtagg ccggcgccca gcttgtcctg
     1441 gtcgcccttc gtcttcggct ctatcgccac cgagatcacc ggctccggaa agtccatcga
     1501 ctcgagccgg atcggcgccg tgtcggtggc gagcgtgtcg ccggtggtcg tgttcttgag
     1561 gcccaccgtc gcggcgattt cgcccgccat gatctgctcc cgctcctcgc ggtgattcgc
     1621 gtgcatctgc aggatccggc cgacgcgctc ggtcttgccc gtcgtcgtgt tcaggacgcg
     1681 gtcgcctgcg ttgacctgcc ctgagtagac ccggatatac gtcagcttgc cgacgtaggg
     1741 gtcggacatg accttgaagg ccagcgccga gaacggctca tcgacggtgg gccggcgcga
     1801 gagctcgtgc tcggtccgtg ggtcgatgcc gtgcaccggt ggcacgtcga gcgggctcgg
     1861 caggtagtcg acgaccgcgt cgagcagcgg ctggacgccc ttgttcttga acgcggagcc
     1921 ggcgagcacc ggagtgacgg tgatgtcgag cgtcgccgcg cggagcgcgc gcttcagcat
     1981 ctccgctgtg acggcgtcct cgtccagcag gtacgtctcg agcagctcgt cgtcgtggtc
     2041 cgcgaccgag tcgatcagct ggtggtggta ctcctgcgcc tgctcgagca gctcggcggg
     2101 aatctcctcg acctcgtagt tcgtgccgag ctcgtcctgc cagacgagcg cgcgcatctc
     2161 gaccaagtcg atgacgccgc ggaagtgctc ctcctggccg agcggcagct gcaccggcac
     2221 cggccgggcg ccgaggcggt cgaccatgga ctgcacggag gcgaagaagt ccgcgccggt
     2281 gcggtccatc ttgttgatga aggcgattcg cggcacgcgg tagcggtcgg cctgccgcca
     2341 gaccgtctcg gactgcggct ccacgcctgc tacggcgtcg aagacagcga tcgcgccgtc
     2401 gagcacgcgc ag
//
LOCUS       DATBRK010000250        16820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2640908, whole genome shotgun sequence.
ACCESSION   DATBRK010000250 DATBRK010000000
VERSION     DATBRK010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2640908"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..946
                     /locus_tag="VIH70_07545"
     CDS             17..946
                     /locus_tag="VIH70_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY8116251.1"
                     /translation="MCGIAGVIHRDGSADIGSEMTSMLQSMKHRGPDSSGYALFGPQT
                     DLIVIRFKLADANDPRDFDFKERLERHRMEIETRLSKIGASIDRVESETEYAYRATVR
                     YDGDVKALADYLEDVPGCEVLSLGHSLEIVKDLGDAQEVSNLYRLAGFEGTHAIGHVR
                     MATESDVDISGAHPYWAYPFSDVAVVHNGQLTNYFQWKRRLEFSGHRFQSECDSEIIA
                     VYLAEKMAGGDSLKDAMDQSLEDLDGVFTYMAVTQDSLGVAKDEMAAKPLVLYESDEL
                     VAMASEEIGIRALIDHEIDTYDPYEGQVLVWSR"
     gene            934..1734
                     /locus_tag="VIH70_07550"
     CDS             934..1734
                     /locus_tag="VIH70_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020025843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GXGXG motif-containing protein"
                     /protein_id="HEY8116252.1"
                     /translation="MEQVASAGERRTTYDARGLSEPVAEVPHVSQIDGAEAVFDAAKL
                     TTRQINLELRSLIYEQGITDVTVKNPGAKHSIGVGILQRCTITFEGSLGYFGVGLIDG
                     PEVRITGRVGWSVCENMMSGVVRIEKNSGSLTGAALRGGDIVVNGSVGARTGIDQKGG
                     TIIVGGDAGSMTAFMMQRGRQIILGDVGPGLGDSMYDGEIYVAGTVKSLGIDCVPGEW
                     TETDTELIERKFRIYGLGSPPEFQKFVCGKVLYNYDNLEPSERKLVIH"
     gene            1749..3161
                     /locus_tag="VIH70_07555"
     CDS             1749..3161
                     /locus_tag="VIH70_07555"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009815078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY8116253.1"
                     /translation="MSAEDVSSQENVLGRSRIFTPEVINDIHVKAELGRYRMRGFSMF
                     KPIPHWDQLMFLPGTLTRFVIEGYREKCETKTVLGARFAKDPIELDIPIYITGMSFGA
                     LSLEAKMALAKGASMAGTATCSGEGGMIPPERDLSIKWYYQNIQSRYGFNPHHLMVAD
                     ACEFFIGQGCKVGLGGHLMGQKVTEQVAEMRSLPPGIDQRSPARHPDWLGPDDLSLKV
                     QEVREATDYRIPIQLKLGAARVYDDVRMAAKCGPDTIYLDGAEGSTGAGPHIATEETG
                     IPLMAAIPEARRALEDVGLADDIDLIVAGGIRNGADVAKCLALGAKAVAIGTTALIAL
                     NCNKEIPGVTDYEGTVGVPAGQCYHCHTGRCPVGITTQDPELRKRLEVDEAAERVYNL
                     LTAMTMELQMLARACGKTDVHSLEPEDLCALTVEAAAMAKVPLAGTNYIPGVTEEATL
                     EELKTMMQKYLADTGQYAGA"
     gene            3167..3601
                     /locus_tag="VIH70_07560"
     CDS             3167..3601
                     /locus_tag="VIH70_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116254.1"
                     /translation="MSDQHLTIKGTLDAEELAGHRFPYQEDRSLVEDIDLDALTPGRD
                     LNWLEDIHLLEEDGKPAVFDRYSNSFLKIYFEIPEGREDEMARKVLMKHLTEGNSYGI
                     QLKVQHAKFPQPKLGPWVAGSETVGTDWRPATLEGWEKPAGH"
     gene            3604..4392
                     /locus_tag="VIH70_07565"
     CDS             3604..4392
                     /locus_tag="VIH70_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY8116255.1"
                     /translation="MGHRPIVGISEGFADYGDYYGFGYGRPLLAAGALPVLLPYYERA
                     DERVELLGRLDGLVLAGGRDVEAWRYGRVEPHPKHLPGQPHLDEIELHYARAAVEAGL
                     PLLAICRGCQVLNVAFGGTLYGDLEEFPEAGADHPGAKWDEWRALVAATLDGAHRPPH
                     PTHPVEIEPGSMLASHLGSRAVVDSYHHQAIQRLGDGLEAVARAPHGVIEAVEMPRAR
                     SFVMGVQWELHEEWQEDERSLGLWRAFVGEAIEHAGTREAATVG"
     gene            4389..4757
                     /locus_tag="VIH70_07570"
     CDS             4389..4757
                     /locus_tag="VIH70_07570"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116256.1"
                     /translation="MNSSIEIVVDAVDSDAAAAFWKQALGYERLYERGAYTALGPPEG
                     DSRPILLIQRVDVVSPGKTAVHLDLRVDDPEAEVARLEGLGARIAWRVDETDRGGSSW
                     TTMAAPHGTLFCVCPVRKEH"
     gene            4759..6093
                     /locus_tag="VIH70_07575"
     CDS             4759..6093
                     /locus_tag="VIH70_07575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8116257.1"
                     /translation="MSLPRSAKVVVIGAGVHGLSTSWHLAKELESRGLGSGEDVVVLD
                     KTGVGAGPSGLACGVIRNNYFQPAMRELMAHSVEVWESDPEAFHYHPVGYLQVAPEVM
                     HEDVAAIHEQQRAIGYASDLIEGEDECRTYMESIFSDWQARNVTVVLHERKGGYAESM
                     PSMLGLRAKGEALGVEVLAPVTVTGFELDGSAVSRVVTDRGTIACERVVVAAGPWVRD
                     LWHMLELPGTVTVRGRDGRDHERPMWTYWCLVEGTLGVDPSYLLDNDGNLPPVVHLDT
                     DAPLLDDTDGSLVTDELWGIYYKPDFNFGGVQGGAMPWVVDQPADEVAVDPYGPESAE
                     FVVGEGFERMWTSALAHAHKRFEGKRGLYRRAPTGGIGAFTPDSFPVFDTFHENVHVI
                     ADSNHGYKMIGVGALAAREVLGERQALFEPFRFSRYARGALHPVSHSPFPWS"
     gene            6098..7075
                     /locus_tag="VIH70_07580"
     CDS             6098..7075
                     /locus_tag="VIH70_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEY8116258.1"
                     /translation="MSRRDDAPVEFDLPIGAFPSWAADALTAGAWEETPPHDEMQIEG
                     EEGEDPVLAFRRTLGMFATGVTVITTASADQVHGMTANAFMSVSLRPPLVLISVDGRA
                     KMHAMLNEGKRIGISVLSEKQEALSDRFAGRPGGTGPEPSFDVVRETPLVEGALAHLV
                     ARVTRSYWGGDHSLFLAFVEYAHYGHGSPLLFHGGHYEALSAPEGSILGALPPAMRER
                     LLSAGEEHVFESGETVIRQGERGGDAFVILGGYARVVRDGREVARLGAGQFFGEVAML
                     DGRPRTADVIAEGGLRCVALSRDTFRRALEAEPELAWRVLEVLAGRIRG"
     gene            complement(7110..7997)
                     /locus_tag="VIH70_07585"
     CDS             complement(7110..7997)
                     /locus_tag="VIH70_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006457026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY8116259.1"
                     /translation="MATDVAADTLERFTDKQVASFWRDGFLVVEQGLIAPRALERLRE
                     RYLPLFDGEYETGIRPDEVNWVKGRDPEDRTRQLCNAWKSDTVIAAQVLSPRLGRLAA
                     QLMGYEGVRILQDNVLWKPPGTKAIGFHQDGSYADYLVPPEMVTCWMSLHETAADAGP
                     IAYVSGSNHWGVSPPSRAQFHAPEDWLAGVRDAQPADVSELEIVPVVVKAGGGAFHHN
                     ATWHGSEPNRSGTVARMALVSHMLPSNARFHETNVDVNYSRYRRRGDLSMDESFFPVL
                     WDRDGYRTPWLSDLPDIER"
     gene            complement(8000..10336)
                     /locus_tag="VIH70_07590"
     CDS             complement(8000..10336)
                     /locus_tag="VIH70_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEY8116260.1"
                     /translation="MAVGGGPSKLLVGGFRAADPSFEQYWVRPGGVTVLAIAPEDRFT
                     VIDVDGGQVAELTMLAPDGSDAAAAIGSRAEAPATVVRALVASMVDGAREVVQDLAGR
                     GLDPSRAMAVRLFDEWSPPGSEQSFRADREGVLIVAAPAGRLVDGAPPPSDLVVEVHR
                     ASPRSTQEVELPPPLAEPRLDFRIDRSTARSYEVKAGEFIQVIDVEGKQCSDFLAFHR
                     RKLDDGIERGLDSTVTRTLMGSAYPMPGLFGKFYDADMDPLVEVVRDTVGRHDTFGLA
                     CNAKYYEDFGYPGHVNCTDNFNGTLVVYEVAKRKGWPALNLFFNTAFNAENVLVSDEP
                     WSRPGDYVLFRAMTDLVCASSACPDDIDPANAWNPTDVHVRVYSPEHQFSAAIAHRVT
                     PESRPVLTRKTGFHPRVEALTKNLVEYNGYWLPSHFSNEGAVAEYWACRERAAIMDLS
                     PLRKFEVLGPDAEALLQSTITRDARRLSVGQITYTALCNEPGGMIDDATVFRLGQDNF
                     RFVGGDDYDGVWLKEQAERRGLHAWVKPSTDQLHNVAVQGPASREILREVVWTPPAQV
                     SLDDLGWFRFLVGRVRAFDGIPIVVSRTGYTGELGYEVWCHPDDAAAVWDAIWEAGEQ
                     RGLAPFGLDALDIVRIEAGLIVAGNEYDQQVDPFEAGIGFAVDLRGDEDFVGRAALQE
                     RQAHPQRTLVGLALEGNESAGHGDLVFVGRQEIGVVTSGTRSPVLKATIALARVAVGF
                     AELGTGVEVGKLDGLQKRIPATVVRFPFYDPDKTRPRS"
     gene            complement(10339..11742)
                     /locus_tag="VIH70_07595"
     CDS             complement(10339..11742)
                     /locus_tag="VIH70_07595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8116261.1"
                     /translation="MSEKMWGSEPFWGLGYDWDPDWVYTDRQKELRATLIELCEQELR
                     ANAKRFDDELMFPRRNLELLGEHGFLGLTVPEEYGGLGEDHVAYSMVCETIARYGCAS
                     TAMCYVMHIGAVNAIMLRETPELVDKYIRPLNSGKIGTLSYSDPETGSHFWYPVSSGA
                     ERSNGGYRVRKKASWTTSGGFADFYVVQTTSPDFSGYDDLSVFVVDGDYVQSQPSLWD
                     ALGLRGNQSGPIEVPNVEIPATQIVGPEGDGANSNDESVDPWFLIGSSSVWNGLALGA
                     IDIAKRHTTRKKHVDVGLRVADYPTIQDYVGEALMDTNASRLFVLSVAQAMDRETDDN
                     TRNPEPGALARGNYLHWAWQIKFEAAKNVAHVVDKMLHACGGSGYKRDMELERYLRDA
                     KAGWVMGPTNEVLRQFVGKAVLLGFESLDYWNQSYNRRAVENEVKKLDAKGKRQLAEQ
                     LLSEASEQEPKEPAKRT"
     gene            complement(11881..13383)
                     /locus_tag="VIH70_07600"
     CDS             complement(11881..13383)
                     /locus_tag="VIH70_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8116262.1"
                     /translation="MAEAAIEERQLLKALHWYDGFVIALCNPGFLIASLGFTLATVGA
                     LGSVILWGISAFVGLLQNWIYSETAAMFPDKPGGISLYAHEGWRSRFNLFGPIAAFGY
                     WIGWSVVLALFGNLIGSLVVAEWFSGQSDKVYYSTPAGDSPFGLAQVIGIGVVFAVWL
                     FNVFGVRPSLAVGYVTGALLMIPLVILIIFPYFTGDWHSSNITYENVADAPFSLFQLS
                     LMWLFLMCWSAYGIEVCASFAPEYHDTKRDTALALRTAAVFSLLVYTLLPLGLGGVVG
                     TAGDSGAYYVTAFKEIVGNTLGSIAIISLLASLVLSMNSATADGGRALYGIARDDMTI
                     KWLYHLNRFHVPARGMTVDLLVNTGFILLMGANNLAILYLSNIGYVFAHVLALSGFLL
                     LRRDRPNWPRPIKVGPAWLPIAGFLALFNLVLVIYGVTQQDLAAYVGFYEFTGFPFFV
                     GVGILLLSVLLFFYRRAVQDRAKITFRDRDVPAMPTTEQMELLREESVPG"
     gene            13513..13875
                     /locus_tag="VIH70_07605"
     CDS             13513..13875
                     /locus_tag="VIH70_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116263.1"
                     /translation="MRGARITVRCDCGETTYLEYGEIWTCPSCGRRWNTTQIPEAEYW
                     GIMRQMRQYRLQVMGVAVTIAIGFVVLGVAMGPQVWLLAPVVITFWLLIFMPRWRRRV
                     RAAARSLPTWKLRPEEGP"
     gene            13872..14372
                     /locus_tag="VIH70_07610"
     CDS             13872..14372
                     /locus_tag="VIH70_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8116264.1"
                     /translation="MSLAPTKVRPGAARRSSGRPVVLATLGVPLDPKAAAFAVDSAVE
                     AGRELVVVNVTKLEPLGLSMILGYDALPEFTPEVSESIQRSAELARSLGVRVERLRIR
                     SPRPVTALLEFIHERRPGLLVFGPERSALRPRPYGKALAALGELTDCLVWIAPGSSSA
                     SGAGRP"
     gene            14369..14674
                     /locus_tag="VIH70_07615"
     CDS             14369..14674
                     /locus_tag="VIH70_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY8116265.1"
                     /translation="MTFLIDCPNCGPREALEFSYGGERTRRAGPDASDRDLAGYLFFR
                     ENVNGWQIEWWLHRDGCRRWFLAERHTGTNEVRRTFWPGDGPGESGDGAQADEASTA"
     gene            14671..>16820
                     /locus_tag="VIH70_07620"
     CDS             14671..>16820
                     /locus_tag="VIH70_07620"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8116266.1"
                     /translation="MSERLPPRPGEAIRRDRPISFGFDGRTVAAFEGDTIGSALAAAG
                     VSITARSFKYHRARGLFCMTGACPNCLMQVDGIPNVRTCTEPVRDGMRVERQNAWPSV
                     DHDVHGWLNTFSFMMPSGFYYKIFHRPRWAWRTVEPFIRSKAGLGKAPKDADHDPREV
                     HNLHADVLVIGAGPAGLAAAAEAANAGASTIVLEERAEPGGEVAELAAEAEAAGVRII
                     AGTPAFGVFGGPVIAAANGDRLYRIRARHLVFATGAVEQAAVFPNNDLPGVMLSSAVD
                     LLVGRYRVLPGRRAVVLTSSAKGHETARTLRDAGADVTVVDLRPDTRPIDGLSVIAGS
                     TIVAAEGRRRVREVVVGTPGEEGGRTIPCDLVVVAGFQAPSTNLLAMTGAELGFHETA
                     QAFLPTQLPPNVHAAGAVAGARSLEGAIAQGRLAGLEAGLALGVAPPDADERIERLRA
                     QAAQDHDHDHPVVLPPEIGSGHGKQFACLCMDVTNKELKTAVTEGFDSMELLKRYTTI
                     TMGPCQGKACMMASQRLCSRATGSSFAQTRPTTARPPWAPVELGTLAGWRRNPRKETT
                     IHDRHVEAGATFMWAGDWRRPHHYTSPEDEVEAVRTRVGVIDVSTLGKFRVEGPDAVA
                     LLERLYPSRFADLALGRIRYGVMLNDEGVIVDDGTVARVGDDEYFATVTTGNTAALER
                     WITWWNADWGLDVRVLNLTGAYAAVNLAGPASRDV"
BASE COUNT         2659 a         5717 c         5963 g         2481 t
ORIGIN      
        1 aaggagctca gcccgtatgt gcggtatcgc aggagtcatc catcgagacg gctcggccga
       61 catcgggtcc gagatgacgt ccatgctcca atccatgaag caccgaggtc cggactcgag
      121 cggctatgcg ttgttcggtc cgcagaccga cctcatcgtg atccgcttca agctcgccga
      181 cgccaacgac ccgcgcgact tcgacttcaa ggaacgcctg gagcgacatc ggatggagat
      241 cgagacgcga ctgtcgaaga tcggcgcctc gatcgacagg gtcgagagcg agaccgagta
      301 cgcctaccgg gcgaccgtgc gctacgacgg tgacgtcaag gcgctcgccg actacctcga
      361 ggacgtcccc ggctgcgagg tgctctcgct cggtcactcc ctcgagatcg tgaaggacct
      421 cggcgacgcg caggaggtct cgaatctgta caggcttgcc ggcttcgagg gcacgcacgc
      481 gatcggacac gtgcggatgg ccaccgagtc cgacgtggac atctccggag cgcacccgta
      541 ctgggcctac ccgttctccg acgtcgccgt ggtgcacaac ggccagctca cgaactactt
      601 ccagtggaag aggcggctgg agttcagcgg gcaccggttc cagtccgaat gcgactccga
      661 gatcatcgcg gtgtacctgg ccgagaagat ggcgggcggg gactcgctga aggacgcgat
      721 ggatcagagc ctcgaagacc tggacggcgt cttcacgtac atggccgtca cgcaggacag
      781 cctcggtgtg gccaaggacg agatggcggc caagccgctc gtgctctacg aatcggacga
      841 gctggtcgcg atggcgtccg aggagatcgg gatccgcgcg ctgatcgatc acgagatcga
      901 cacctacgac ccgtacgaag ggcaggtgct cgtatggagc aggtagccag cgccggcgag
      961 cgccggacga cgtacgacgc ccggggcctg tccgaacccg tcgccgaggt cccccacgtc
     1021 tcccagatcg acggggccga ggccgtgttc gacgccgcca agctcacgac ccgccagatc
     1081 aacctcgagc tgcgcagcct gatctacgag caggggatca ccgacgtgac ggtgaagaac
     1141 cccggcgcca agcactcgat cggcgtgggg atcctgcagc gctgcacgat cacgttcgag
     1201 gggtcgctcg gctacttcgg cgtggggctg atcgacggcc ccgaggtgcg gatcacggga
     1261 cgggtcggct ggtcggtctg cgagaacatg atgtccggcg tcgtgcggat cgagaagaac
     1321 tcgggatccc tcaccggggc ggccctccgc ggcggcgaca tcgtggtgaa cgggtcggtc
     1381 ggggctcgga ccggcatcga ccagaagggc ggcacgatca tcgtgggcgg cgacgccggc
     1441 tcgatgaccg cgttcatgat gcagcgcggg cgccagatca tcctgggcga cgtgggtccg
     1501 ggcctgggcg actcgatgta cgacggcgag atctacgtcg caggcacggt gaagtcgctc
     1561 gggatcgact gcgtgccggg ggagtggacc gagaccgaca ccgagctgat cgagcggaag
     1621 ttccgcatct acggtttggg ctcgccgccc gaattccaga agttcgtctg cggcaaggtc
     1681 ctgtacaact acgacaacct cgaaccgtcc gagcgcaagc tcgtcatcca ctaggaggcc
     1741 tgcgaaccgt gtcagccgaa gacgtgtcct cccaagagaa cgtgctgggt cgcagccgca
     1801 tcttcacgcc ggaggtgatc aacgacatcc acgtgaaggc ggagctcggc cggtaccgga
     1861 tgcgagggtt ctcgatgttc aagccgatcc cgcactggga ccagttgatg ttcctgcccg
     1921 ggacgctcac taggttcgtg atcgagggct accgcgagaa gtgcgagacc aagacggtgc
     1981 tcggcgcacg gttcgcgaag gacccgatcg agctcgacat cccgatctac atcaccggga
     2041 tgagcttcgg cgcgctgtcc ctggaggcga agatggcgct cgccaagggg gcgtcgatgg
     2101 cggggaccgc cacgtgctcg ggcgagggag ggatgatccc ccccgaacgc gacctgtcga
     2161 tcaagtggta ctaccagaac atccagagcc ggtacgggtt caacccgcac cacctcatgg
     2221 ttgccgacgc gtgcgagttc ttcatcggcc agggctgcaa ggtcggcctg ggcggccacc
     2281 tgatggggca gaaggtcacg gaacaggtgg ccgagatgcg ctcgctcccg ccgggcatcg
     2341 accagcgctc gccggcgcgg cacccggact ggctcggacc cgacgacctc tcgctcaagg
     2401 tgcaggaggt gcgcgaggcg acggactacc ggatcccgat ccagctgaag ctcggcgcgg
     2461 cgcgcgtcta cgacgacgtt cggatggccg ccaagtgcgg gcccgacacc atctacctcg
     2521 acggcgccga ggggtccacc ggcgcggggc cgcacatcgc gaccgaggag accggcatcc
     2581 cgctgatggc ggcgatcccc gaggcccggc gggcgctgga ggacgtcggc ctcgccgacg
     2641 acatcgacct gatcgtcgcc ggcgggatcc gcaacggcgc cgatgtggcg aagtgcctcg
     2701 cgctcggcgc gaaggcggtc gcgatcggga cgacggcgct gatagcgctg aactgcaaca
     2761 aggagatccc gggtgtcacc gactacgagg gcacggtcgg cgtccctgcc ggccagtgct
     2821 accactgcca cacgggccgg tgcccggtcg gcattaccac gcaggacccc gagctccgca
     2881 agcgtctcga ggtggacgag gccgccgagc gcgtctacaa cctgctcacc gcgatgacga
     2941 tggagctgca gatgctcgcc cgggcgtgtg gcaagaccga cgtgcactcg ctcgagcccg
     3001 aggacctgtg cgcgctcacg gtggaggcgg cggcgatggc caaggtgccg ctcgccggca
     3061 ccaactacat ccccggcgtc accgaggagg ccacgctcga ggagctcaag acgatgatgc
     3121 agaagtacct ggctgacacc ggccagtacg cgggggcgtg atccggatga gcgaccagca
     3181 cctgacgatc aaggggacgc tcgacgccga ggagctcgcg ggccatcggt ttccgtacca
     3241 ggaggaccgg tcactcgtcg aggacatcga cctggacgcg ctcacgcccg gccgggacct
     3301 caactggctc gaggacatcc acctgctcga ggaggacggc aagccggccg tcttcgaccg
     3361 ctattcgaac tcgttcctga agatctactt cgagatcccg gaggggcgcg aggacgagat
     3421 ggcccgcaag gtgctgatga agcacctgac cgagggcaac tcgtacggca tccagctgaa
     3481 ggtgcagcac gcgaagttcc cccagcccaa gctcgggccg tgggtggcgg gttccgagac
     3541 cgtcgggacc gactggcggc ccgccacgct ggagggctgg gagaagcccg ccggacactg
     3601 agcgtgggcc accgaccgat cgtcgggatc tcggagggtt tcgccgacta cggtgactac
     3661 tacgggttcg ggtacggacg gccgctgctc gcggccggcg cgctcccggt gttgctgccg
     3721 tactacgagc gggccgacga gcgcgtcgag ctgctcggcc ggctcgacgg gctcgtgctc
     3781 gccggtggcc gggacgtcga ggcctggagg tacggccgcg tggaaccgca cccgaagcac
     3841 ctgcccggcc aacctcacct cgatgagatc gagctgcact acgcgcgtgc ggccgtcgag
     3901 gccgggctcc cgctgctcgc gatctgtcgc ggatgtcagg tgctgaacgt cgcgttcggc
     3961 ggcacgctct acggcgatct cgaggagttc cccgaggcag gggccgacca tccgggcgcg
     4021 aagtgggacg agtggcgtgc cctcgtggcg gcgaccctcg acggggccca tcgcccgccg
     4081 cacccgacgc atcctgtcga gatcgaaccg gggtcgatgc tcgcgtcgca cctcgggtcg
     4141 cgcgccgtgg tcgacagcta tcaccatcag gcgatccaac ggctcggcga tggactcgag
     4201 gccgtcgccc gagccccgca tggtgtgatc gaggcggtcg agatgccgcg ggcccgatca
     4261 ttcgtcatgg gcgtccagtg ggagctgcac gaggagtggc aggaggacga gcgctcgctc
     4321 ggcctgtggc gcgcgttcgt cggggaggcc atcgagcacg ccggcacccg agaggcggcg
     4381 acggtcggtt gaactcctcg atcgagatcg tcgtggacgc ggtcgactcc gacgccgcgg
     4441 ccgccttctg gaagcaggcc ctgggctacg agcggctcta cgagcggggc gcgtacaccg
     4501 cgctcggacc gcccgagggg gacagccgtc ccatcctgct gatccagcgc gtggacgtcg
     4561 tgtcgccggg caagaccgcc gtccacctgg accttcgcgt cgacgatccc gaggccgagg
     4621 tggcgcgcct ggagggcctc ggcgcccgga tcgcgtggcg cgtcgacgag accgaccgag
     4681 gtggctcgag ctggacgacg atggccgcac cgcacgggac cctgttctgc gtctgccccg
     4741 tccgcaagga gcactagcgt gtcgttgccg cggagcgcga aggtcgtcgt gatcggggcc
     4801 ggcgtccacg ggctctcgac gtcctggcac ctggcgaagg agctcgagtc gcgggggctc
     4861 ggctcgggcg aggacgtcgt cgtgttggac aagaccggcg tcggcgccgg tccgtcgggg
     4921 ctcgcgtgcg gggtgatacg caacaactac ttccagcccg cgatgcgcga gctgatggcg
     4981 cactcggtgg aggtctggga gtccgatccg gaggcgttcc actaccaccc ggtcgggtac
     5041 ctgcaggtcg cgcccgaggt gatgcacgag gacgtcgccg cgatccacga gcagcagcgg
     5101 gcgatcggct acgcctcaga cctgatcgag ggcgaggacg agtgccgcac ctacatggag
     5161 tcgatcttct cggactggca ggccaggaac gtgacggtcg tcctgcacga gaggaagggc
     5221 ggctacgccg agagcatgcc ctcgatgctc ggcctccgcg ccaaggggga ggcgctcggc
     5281 gtcgaggtgc tcgcgccggt gaccgtgacc gggttcgagc tcgacgggag cgcggtctcg
     5341 agggtcgtca cggaccgggg caccatcgcg tgtgagcggg tcgtggtcgc cgcgggcccg
     5401 tgggtgcgcg acctctggca catgctggag ttgccgggga ccgtgacggt caggggccgg
     5461 gacggccgag accacgaacg gccgatgtgg acctactggt gcctggtcga ggggacgctg
     5521 ggcgtcgatc cctcctacct gctcgacaac gacggcaacc tgccgcccgt cgtccacctc
     5581 gacaccgacg ccccgctgct cgacgacacc gacggctcgc tcgtgaccga cgagctctgg
     5641 ggcatctact acaagccgga cttcaacttc ggcggcgtgc agggcggggc gatgccgtgg
     5701 gtcgtcgacc agccggccga cgaggtggcg gtcgacccct acgggcccga gagcgccgag
     5761 ttcgtcgtgg gggaggggtt cgagcggatg tggacatcgg cgctcgcgca cgcgcacaag
     5821 cggttcgagg gcaagcgcgg gctctatcgc cgggcaccta ccggtgggat cggcgcgttc
     5881 accccagaca gcttccccgt gttcgacacg ttccacgaga acgtgcacgt gatcgcggac
     5941 tcgaaccacg gctacaagat gatcggcgtg ggcgcgctcg cggcgaggga ggtgctgggg
     6001 gagcgccagg cgctgttcga gccgttccga ttctcccggt acgcccgggg agcgctccac
     6061 cccgtgagcc actcaccctt cccctggagc tagcggcatg agccggcggg acgacgcccc
     6121 cgtcgagttc gacctcccga tcggcgcgtt cccgtcgtgg gccgccgacg cgctcaccgc
     6181 cggcgcgtgg gaggagacgc ccccgcacga cgagatgcag atcgagggcg aggagggcga
     6241 ggacccggtc ctcgcgttcc gacggacgct cgggatgttc gcgaccggcg tcacggtaat
     6301 cacgacggcc tccgccgacc aggtgcacgg gatgacggcc aacgcgttca tgtcggtctc
     6361 gctccggccg cccctcgtcc tgatctcggt cgacgggcgc gcgaagatgc acgcgatgct
     6421 gaacgagggc aagcggatcg ggatcagcgt gctttcggag aagcaggagg cgctgtcgga
     6481 tcgcttcgcc gggcggccgg gtggcaccgg gcccgagccg agcttcgacg tcgtccgcga
     6541 gacgccgctc gtcgagggcg ccctcgcgca tctggtcgcg agggtcacgc gctcgtactg
     6601 gggaggcgac cactcgctgt tcctcgcgtt cgtggagtac gcccactacg ggcatgggag
     6661 cccgctcctg ttccacggcg gccactacga ggcgctctcg gccccggagg gctcgatcct
     6721 cggcgcgctc ccgccggcga tgcgcgagag gctgctgtcg gccggcgagg agcatgtgtt
     6781 cgagtccggg gagacggtga tccgtcaggg ggagcgcggc ggcgacgcgt tcgtcatcct
     6841 cggcgggtat gcccgtgtcg tgcgggacgg gcgggaggtc gcccggttgg gtgccggcca
     6901 gttcttcggc gaggtggcga tgctcgacgg gaggccccgg accgccgacg tcatcgccga
     6961 gggtggtctg cggtgcgtcg cgctgtcgcg ggacaccttc aggcgtgcgc tcgaggccga
     7021 acccgagctc gcctggcgcg tcctcgaggt cctcgccggc cggatccgcg gctgacccgg
     7081 accggggcac gggattgcgg tgacgagcgt cagcgctcga tgtcgggcag gtccgacagc
     7141 cacggcgtgc ggtagccgtc gcgatcccac agcaccggga agaacgactc gtccatcgag
     7201 aggtcgccgc gtcggcggta ccgcgagtag ttcacgtcca cgttcgtctc gtggaatcga
     7261 gcgttcgagg gcagcatgtg cgagacgagc gccatccggg ccaccgtgcc gctgcggttg
     7321 ggttcggagc cgtgccacgt cgcgttgtgg tggaacgcgc cgcctccggc cttcaccacg
     7381 accggcacga tctcgagctc ggacacgtcc gcgggctggg cgtcccgaac tcccgccagc
     7441 cagtcctcgg gcgcgtggaa ctgcgcccgc gagggaggag agacgcccca gtggttcgag
     7501 ccgctaacgt acgcgatcgg accggcgtcg gcggccgtct cgtgcaggga catccagcac
     7561 gtcaccatct cgggcggcac gagatagtcg gcgtacgacc catcctggtg gaacccgatc
     7621 gccttggtcc ccggcggctt ccacagcacg ttgtcctgca ggatccgaac cccctcgtac
     7681 cccatcagct gcgccgctag ccggccgagt cgcggcgaga gcacctgcgc cgcgatcacc
     7741 gtgtccgact tccacgcgtt gcagagctgc cgggttcgat cctccggatc ccggcccttg
     7801 acccagttca cctcgtcggg ccggatcccg gtctcgtact cgccgtcgaa cagcggaagg
     7861 tagcgctcgc ggaggcgttc gagcgcgcgc ggggcgatca ggccctgctc gacgaccagg
     7921 aatccgtcgc gccagaacga cgcgacctgc ttgtcggtga agcgttcgag cgtatcggcg
     7981 gcgacgtcgg tcgccacgcc taggacctcg gccgcgtctt gtccgggtcg tagaacggga
     8041 accggaccac cgtggcgggg atccgcttct gcaggccgtc gagcttgccg acctcgacgc
     8101 cggtcccgag ttccgcgaac cccaccgcca cccgcgcgag cgcgatcgtg gccttcagca
     8161 ccggggagcg ggtccccgag gtcacgacgc cgatctcctg tcgcccgacg aagacgaggt
     8221 caccgtgccc ggccgactcg ttgccttcca gcgccagccc gacgagcgtc cgctgcggat
     8281 gggcctggcg ctcctggagg gcggcgcgcc cgacgaagtc ctcgtccccc cgcaggtcga
     8341 cggcgaaccc gatgcccgcc tcgaacgggt cgacctgctg gtcgtactcg ttgccggcca
     8401 cgatcagccc ggcctcgatc cgaacgatgt cgagggcatc caggccgaac ggcgcgagcc
     8461 cgcgctgctc gccggcctcc cagatcgcgt cccagacggc cgcggcgtcg tcgggatggc
     8521 accagacctc gtacccgagc tcgcccgtgt accccgtacg agagacgacg atcgggatcc
     8581 cgtcgaacgc tcgcacccga cccacgagga accggaacca ccccagatcg tcgagcgaca
     8641 cctgggcggg cggggtccac acgacctcgc gcaggatctc gcggctcgcc ggcccctgca
     8701 cggcgacgtt gtggagctgg tcggtcgagg gcttgaccca ggcgtgcagt ccgcgccgct
     8761 cggcctgctc cttcagccac acgccgtcgt agtcgtcgcc tcccacgaac cggaagttgt
     8821 cctgaccgag gcggaacacc gtggcgtcgt cgatcatccc gccgggctca ttgcacagcg
     8881 ccgtgtaggt gatctggccg accgagagcc gccgcgcgtc cctcgtgatc gtcgactgca
     8941 gcaatgcctc ggcgtcgggc ccgaggacct cgaactttcg caggggcgac aggtccatga
     9001 tcgcggcccg ctcgcgacac gcccagtact cggcgaccgc gccctcgttg gagaagtgcg
     9061 aggggagcca gtacccgttg tactcgacga ggttcttcgt gagcgcctcg accctcgggt
     9121 ggaacccggt cttcctggtg agcaccggcc gggactcggg ggtcacgcga tgggcgatcg
     9181 ccgcgctgaa ctggtgctcg ggcgagtaga cgcggacgtg gacgtcggtc ggattccacg
     9241 cgttcgccgg gtcgatgtcg tcggggcagg cagacgaggc gcacacgagg tcggtcatcg
     9301 cccggaacag gacgtagtcg ccgggacgcg accagggctc gtcggagacg agcacgttct
     9361 cggcgttgaa cgccgtattg aagaacaggt tgagcgcggg ccagcccttg cgcttggcga
     9421 cctcgtacac gacgagcgtc ccgttgaagt tgtcggtgca gttcacgtgg ccggggtagc
     9481 cgaagtcctc gtagtacttg gcgttgcacg ccaggccgaa cgtgtcgtgc cgcccgaccg
     9541 tgtcacgcac gacctcgacg agcgggtcca tgtcggcgtc gtagaacttc ccgaacaggc
     9601 ccggcatggg gtacgcgctg cccatcagcg ttcgggtgac ggtggaatcc aggccgcgct
     9661 cgatgccgtc gtcgagcttt cggcgatgga aggcgaggaa gtccgagcac tgcttgccct
     9721 cgacgtcgat cacctggatg aattcgccgg ccttgacctc gtagctgcgt gccgtggacc
     9781 ggtcgatccg gaagtccagg cggggttcgg cgagcggggg cgggagctcg acctcctgcg
     9841 tcgaacgggg gctcgcgcgg tgcacctcga cgacgaggtc gctcggcggg ggagcgccgt
     9901 cgacgagccg gccggccggg gcggccacga tgagcacgcc ctcgcggtcg gcgcggaacg
     9961 actgttcgga ccccggcggg gaccactcgt cgaagagccg gacggccatc gcgcggctcg
    10021 ggtccagccc gcggccggcg agatcctgca cgacctcccg ggcaccgtcg accatcgagg
    10081 cgaccagtgc ccggaccacg gtcgcggggg cctcggcccg cgagccgatc gccgccgccg
    10141 cgtccgagcc gtcgggggcc agcatcgtga gctcggccac ctggccgccg tcgacgtcga
    10201 tcacggtgaa ccggtcctcc ggcgcgatcg ccagcaccgt cacgccgccc ggacgcaccc
    10261 agtactgctc gaaggacggg tcggccgcgc ggaagcctcc cacgaggagc ttcgacgggc
    10321 cgccgccgac cgccatgcct aggtcctctt cgccggttcc ttgggctcct gctccgaggc
    10381 ctccgacagc agctgctcgg ccagctggcg cttgcctttg gcgtcgagct tcttcacctc
    10441 gttctcgacc gcccggcggt tgtacgactg gttccagtag tcgagcgact cgaaccccag
    10501 gagcacggcc ttgcccacga actggcggag cacctcgttc gtcggcccca tcacccagcc
    10561 cgccttggcg tcgcggaggt agcgctcgag ctccatgtcc cgcttgtagc ccgagccgcc
    10621 gcacgcgtgc agcatcttgt cgaccacgtg cgccacgttc ttggcggcct cgaacttgat
    10681 ctgccacgcc cagtggaggt agttgccccg ggccagggcc ccgggttccg gattccgggt
    10741 gttgtcgtcg gtctcgcggt ccatcgcctg ggcgaccgac agcacgaaca ggcgggaggc
    10801 gttcgtgtcc atcagcgcct cgccgacgta gtcctggatc gtggggtagt cggcgacgcg
    10861 caggcccacg tccacgtgct tcttgcgcgt tgtgtgccgc ttggcgatat cgatcgcgcc
    10921 gagcgcgagc ccgttccaca cggaggagga gccgatcagg aaccaggggt ccaccgactc
    10981 gtcgttcgag ttcgcgccgt caccctcggg ccccacgatc tgggtggcgg ggatctcgac
    11041 gttcgggacc tcgatcgggc cggactggtt cccgcgcagg ccgagcgcgt cccagagcga
    11101 cggctgcgat tgcacgtagt cgccgtcgac gacgaagacc gagaggtcgt cgtagccgga
    11161 gaagtccggg ctcgtggtct ggaccacgta gaagtcggcg aacccgccgg acgtggtcca
    11221 ggaggccttc ttgcggaccc ggtagccgcc gttggatcgc tcggcgcccg aggagaccgg
    11281 ataccagaag tgtgagccgg tctcggggtc cgagtacgag agggtcccga tcttgcccga
    11341 gttgagcggg cggatgtact tgtccaccag ctcgggcgtc tcgcgcagca tgatcgcgtt
    11401 cacggcgccg atgtgcatca cgtaacacat cgcggtcgag gcgcagccgt acctcgcgat
    11461 cgtctcgcac accatcgagt acgcgacgtg gtcctcgccc aggcccccgt actcctcggg
    11521 gacggtgagg ccgaggaacc cgtgctcccc gaggagctcg aggttgcgac gcgggaacat
    11581 cagctcgtcg tcgaaccgct tggcattggc gcgcagctcc tgctcgcaga gctcgatcag
    11641 ggtcgcgcgg agctccttct gccgatcggt gtagacccag tccggatccc agtcgtaccc
    11701 caggccccag aacggttccg aaccccacat cttctccgac atcggctcct ccagtgcgtg
    11761 gcccgcgggc ggggcttcca tcctagtttc gaaggctgta cctggacaac gcggacgagt
    11821 ccgggacgcg cgacggggcg gccggtggcc accccgtcgc cgaaccgagc ccgtcggacg
    11881 ctaccccggg accgactcct cccgtaggag ctccatctgc tcagtcgtcg gcatcgccgg
    11941 gacgtcgcgg tcgcggaacg tgatcttggc ccggtcctgc accgcccgcc ggtagaagaa
    12001 caggagcacg gacaggagca ggatcccgac cccgacgaag aacgggaagc cggtgaactc
    12061 atagaagccg acgtacgcgg ccaggtcctg ctgcgtgacg ccgtagatca ccagcacgag
    12121 attgaacagg gccaggaacc cggcgatcgg gagccacgcc ggcccgacct tgatcggcct
    12181 cggccagttg ggccggtctc gccggagcaa caggaacccg ctcagcgcga ggacgtgcgc
    12241 gaacacgtag ccgatgttgg acaggtagag gatcgccagg ttgttcgccc ccatgaggag
    12301 gatgaacccg gtgttgacga gcagatcgac ggtcatgcca cgtgccggca cgtggaaccg
    12361 gttgaggtgg tacagccact tgatcgtcat gtcgtcgcgc gcgatcccgt acaacgcgcg
    12421 gcctccgtcg gcggtcgccg agttcatcga cagaaccagg ctcgcgagca gcgagatgat
    12481 cgcgatgctg ccgagcgtgt tgccgacgat ctccttgaac gccgtgacgt agtaggcccc
    12541 cgagtcgccg gccgtgccaa ccacgccgcc aagtcccagc gggagcagcg tgtacacgag
    12601 gagcgagaac acggcggccg tgcgcagcgc gagcgcggtg tcccgcttgg tgtcgtggta
    12661 ctcgggtgcg aagctcgcgc acacttcgat cccgtatgcg gaccaacaca tcaggaacag
    12721 ccacatcagc gagagctgga acaggctgaa cggcgcatcg gcgacgttct cgtaggtgat
    12781 gttcgagctg tgccagtcgc ccgtgaagta cgggaagatg atcaggatca ccagcgggat
    12841 catgagcaac gccccggtca cgtacccgac ggcgagcgag ggtctcacgc cgaatacgtt
    12901 gaagagccac acggcgaaca cgaccccgat cccgatcacc tgcgccagac cgaagggcga
    12961 gtcgccggcc ggggtgctgt agtagacctt gtcgctctgc ccggagaacc actcggccac
    13021 caccagcgag ccgatcaggt tcccgaacaa ggcgagcacg acggaccacc cgatccagta
    13081 tccgaaggcg gcgataggac cgaacaggtt gaaccggctc cgccagccct cgtgcgcgta
    13141 aagcgagatc ccgccgggct tgtcggggaa catcgcggcg gtctcggagt agatccagtt
    13201 ctggagcaac ccaacgaacg ccgagatgcc ccacaggatc actgagccca acgcgccgac
    13261 cgtcgcgagg gtgaacccca acgaggcgat caggaatccc ggattgcaca gggcgatgac
    13321 gaacccgtcg taccagtgga gcgccttgag cagctgccgc tcctcgatgg ctgcctcagc
    13381 cacgcgacac ctccttgacc acgatcggcg ggcccctccc gtagggtgtc ggcgcgggta
    13441 tagcacggtc ccgagcaatc gttcaaacgc gttcgcacgt tcggcctgcg cgccgcggag
    13501 taccatccac gcatgcgggg cgcccggatc acggttcgct gcgactgcgg agagaccacc
    13561 tacctcgagt acggcgagat ctggacatgc ccctcgtgcg gccggcgctg gaacaccacc
    13621 cagatccccg aggccgagta ctgggggatc atgcgccaga tgcggcagta ccgcctccag
    13681 gtcatgggtg tggccgtcac gatcgcgatc gggttcgtgg tgctcggcgt cgcgatgggc
    13741 ccgcaggtct ggctgctcgc ccccgtcgtg atcacgttct ggctgttgat cttcatgccg
    13801 aggtggcggc ggagggtgcg ggccgcggct cggagtctgc cgacctggaa gctccgtccc
    13861 gaggagggac cgtgagtctc gcgccgacga aggtccgccc cggcgccgcg aggcgttcct
    13921 cgggccgacc ggtcgtgctc gcgaccctgg gggtgccgct cgacccgaaa gcggcggcgt
    13981 tcgcggtcga cagcgccgtt gaggccgggc gcgagctcgt cgtggtgaac gtcaccaagc
    14041 tcgaaccgct cggcctctcg atgatcctgg ggtacgacgc cctgcccgag ttcacgcccg
    14101 aggtctccga gtcgatccag cgctcggccg agctcgcgcg gtcgctcggc gtccgcgtgg
    14161 agcggctgcg gatccggagc ccccgccccg tcacggcgtt gctcgagttc atccacgagc
    14221 gacgtccggg cctgctcgtg ttcgggccag aacgctcggc gctgcgacca cgcccgtacg
    14281 gcaaggcgct cgccgcgttg ggcgagctca ccgactgcct ggtctggatc gcgcccggga
    14341 gttcctccgc gtcgggggcc gggcgaccgt gaccttcctc atcgactgcc cgaactgcgg
    14401 gccgcgagaa gcgctcgagt tctcctacgg cggcgaacgc actcgccggg ccggtccgga
    14461 tgcatcggac cgagacctcg ccggctacct cttcttccga gagaacgtca acggctggca
    14521 gatcgagtgg tggctccacc gcgacggctg ccggaggtgg ttcctcgccg agcggcacac
    14581 cggcacgaac gaggtccggc ggacgttctg gcccggcgac ggacccggcg agtccggcga
    14641 cggcgcacag gccgacgagg cctcgaccgc gtgagcgaac ggctcccacc gcgacccggc
    14701 gaagccatcc ggcgcgatcg cccgatctcg ttcgggttcg acgggcggac ggtcgccgcg
    14761 ttcgagggtg acacgatcgg ttccgcgctg gcggcggcgg gcgtctcgat cacggcacgt
    14821 tcgttcaaat accaccgcgc gcgcggcctg ttctgcatga ccggggcgtg tccgaactgc
    14881 ctgatgcagg tggacgggat cccgaacgtc cgcacatgca ccgagccggt ccgcgacggg
    14941 atgcgggtcg agcgccagaa cgcgtggccg agcgtcgacc acgacgtcca cggctggctg
    15001 aacacgttct cgttcatgat gccgtcgggc ttctactaca agatcttcca ccggcctcgg
    15061 tgggcgtgga ggacggtcga gcccttcatc cgctcgaagg ccggcctcgg caaggcgccg
    15121 aaggatgcgg atcacgaccc gcgcgaggtg cacaacctgc acgccgacgt cctggtgatc
    15181 ggcgccggcc ccgcgggcct cgcggcggcg gccgaggcgg cgaacgcggg cgcctcgacg
    15241 atcgtgctgg aggaacgcgc cgagccgggg ggcgaggtcg ccgagctggc ggcggaggcc
    15301 gaagccgccg gcgtgcggat catcgcgggg acgccggcgt tcggggtgtt cggcggtccg
    15361 gtgatcgccg ccgcgaacgg cgaccggctc taccggatcc gcgcgcggca cctcgtgttc
    15421 gcgaccggtg cggtcgagca ggccgcggtg ttcccgaaca acgacctgcc gggcgtgatg
    15481 ctctcgtcgg cggtcgacct gctggtgggg cgctaccgcg tgctacccgg acggcgggcc
    15541 gtggtactca cctcgagcgc gaagggccac gagaccgctc gcaccctgcg cgatgccggt
    15601 gccgacgtga cggtggtcga cctgcggccc gacacgcgac cgatcgacgg gctgagcgtg
    15661 atcgccggat ccacgatcgt cgccgcggag ggacggcgcc gcgtccgaga ggtcgtggtc
    15721 gggaccccgg gcgaggaggg gggccggacg atcccgtgcg atctggtggt ggtcgcgggc
    15781 ttccaggccc cctcgacgaa cctgctcgcg atgaccggtg ccgagctcgg gttccacgag
    15841 acggcgcagg cgttcctgcc cacgcagctg cccccgaacg tccacgcggc cggtgcggtc
    15901 gccggcgcgc gctcgctcga aggcgcgatc gcgcagggcc ggctcgccgg gctcgaggcc
    15961 ggactcgccc tcggtgtcgc cccgcccgac gcggacgaac ggatcgaacg gctccgcgcc
    16021 caggcggccc aggaccatga ccacgaccat cccgtcgtcc ttcctccgga gatcggatcc
    16081 ggacacggca agcagttcgc gtgtctgtgc atggacgtca cgaacaagga gctcaagacc
    16141 gcggtgaccg aggggttcga ctcgatggag ctcctcaagc ggtacacgac gatcacgatg
    16201 ggtccgtgcc agggcaaggc ctgcatgatg gcctcgcagc gcctgtgctc gagggcgacc
    16261 ggctcgtcgt tcgcgcagac ccgcccgacc acggcccggc cgccgtgggc gccggtcgag
    16321 ctcgggacgc tcgccggctg gcggcggaac ccccgcaagg agaccacgat ccacgaccgg
    16381 cacgtggagg ccggcgcgac gttcatgtgg gcgggcgatt ggcgccggcc gcaccactac
    16441 acctcgccgg aggacgaggt cgaggctgtg cggacgcggg tgggcgtgat cgacgtgagc
    16501 acgctcggca agttccgcgt cgagggcccg gacgcggtcg cgctgctgga gcgcctgtac
    16561 ccgagccggt tcgccgacct cgcgctcggg cggatccgct acggcgtgat gctgaacgac
    16621 gagggggtga tcgtggacga cgggaccgtc gccagggttg gcgacgacga gtacttcgcc
    16681 accgtcacca ccgggaacac cgcggcgctc gagcgctgga tcacgtggtg gaacgccgac
    16741 tggggtctgg acgtccgcgt gctgaacctc acgggcgcct acgcggcggt gaacctcgcc
    16801 ggtcccgcgt cgcgcgacgt
//
LOCUS       DATBRK010000251         7176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2641272, whole genome shotgun sequence.
ACCESSION   DATBRK010000251 DATBRK010000000
VERSION     DATBRK010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641272"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VIH70_07625"
     CDS             complement(<1..195)
                     /locus_tag="VIH70_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8116267.1"
                     /translation="MKHVRIATYEIKKGSFQELADVAQDGMLREFRNQPGFIRYGLAD
                     SGDKTCVSLSLWETREDADAA"
     gene            659..871
                     /locus_tag="VIH70_07630"
     CDS             659..871
                     /locus_tag="VIH70_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116268.1"
                     /translation="MRPRDPEATTNFFRTELGKTLRVDETDEGAISVDILKEGVWSEA
                     PRGMIGLRLSPGSRGLTATEILALPT"
     gene            complement(925..4470)
                     /locus_tag="VIH70_07635"
     CDS             complement(925..4470)
                     /locus_tag="VIH70_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8116269.1"
                     /translation="MPTPRTCPDCSAANPAEARFCLNCGAKLHDSTAVDPDTHLEPYL
                     PKELLAKLEAARAGRAMEGERRIVTMLFCDVKGSTAMAETLDPEDWAEIMNGAFERLI
                     APVYRYEGTLARLMGDAIFAFFGAPIAHEDDPQRAVQAGLDIIAGIAQYKERISAERG
                     LDLDVRVGINTGPVMVGQVGSDLRVEYTAMGDAVNVAARMEQTAEPGTVQITAETHRL
                     VDSLFDLESRGGIEVKGKAEPVSVFRVLGRKAGVTEARSLRGSPLVGREREMEWLRDA
                     IDEAQSGGGRIVSLIGEAGLGKSRLVQETVTEWTRRRPEDSSRVGREIHRLWETWQCV
                     SYDATRPYAQYRRMLARLAAIEDTDPPDVVRRKLAATMEPGSEEEWLEPHMRVWRSLF
                     GVTEPGEEPLEGQAFRDAIMELVPGATRAFGADEPRLLVFEDLHWCDEASMDVLIETA
                     KLVDELPCLFLFAYRPDRQAPSWRLKQWLETEYPHRSTELALDPLSDDESGRLIDALI
                     PDEDPAVRAQILERTDGNPLFVEEVAAALLEHHDDISIPTTLQALFTARLDALDEQAK
                     HLLQIASVIGRSFSGPVLRAVAGEDDLTSSLRTLERVGLISETARTPDRGYAFHHSLT
                     QDATYGTILLRRRRELHLRVGEVFEERYANRLDEFAPVLAYHFREAGDDERTLRYATI
                     AGDNAGRLYANAEAATQYATAIDAAKRRGATADSIGHLHPSRGRVLELSGRYDEAVAT
                     YEEMATLAREAGNPGGVLGAEMALTTIHATPTPVFDSSKGRVLAERTIALARELGDRS
                     AESKALWNLMILNVFGGGDADEAVEAGERSLEIARELNAREQIAFTLNDLWRPYAAVG
                     DVDAARSCLEEARPIWREIGNLPMLCENLIGTAALLALAGEPEEALALYDEAYAISEE
                     IGNPWGQAYALFNAYLVDVEQGNVGRAMARMRECIQRSEESGFLIPQSAARADLGVQY
                     ARLGQFERGIELADRGLEIAKGQTPLAIPMAMVSKAEILFLAGESEQAESLIAEADLG
                     RLPGPVRGAAGAHVDILRGQLAAARGNHVEAVEIADEVIAWLHRHELRQFLPAALLLK
                     SRELIASERPGEADPLLREARSRAEDMGFLRLLWEIDLELSGVAADRGDVGEAAALRA
                     EAASTVSAISLTIDDEELRASFLALPDVRAALSER"
     gene            complement(4613..5044)
                     /locus_tag="VIH70_07640"
     CDS             complement(4613..5044)
                     /locus_tag="VIH70_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEY8116270.1"
                     /translation="MATKQARVSWKGDLENGTGTIEQVGSGAIGALAVSWAARTGDES
                     GMTSPEELIAAAHASCFSMAFSARLAKAGTPPDRLEVSAAVTFVPGSGITTSALDVTG
                     VVPGISEEDFDTLAEDAKENCPVSSALKGNVEMSVTARLAS"
     gene            5176..5505
                     /locus_tag="VIH70_07645"
     CDS             5176..5505
                     /locus_tag="VIH70_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HEY8116271.1"
                     /translation="MPQRAWSAKRERQYEHIKQGLEERGRDEDTAEEIAARTVNKERA
                     RSGEARTSSPTSTRDVSSGRRGGLRSHRGPGGRTKAQLYREARDKHVEGRSKMTKAQL
                     QRAVDRD"
     gene            5518..5709
                     /locus_tag="VIH70_07650"
     CDS             5518..5709
                     /locus_tag="VIH70_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116272.1"
                     /translation="MPSVQGLSKTQANYRKAENPQFRCGECKFMFPRLSIGGCRYVRG
                     VIHNDDTCDEFKPRDAGSS"
     gene            complement(5737..6273)
                     /locus_tag="VIH70_07655"
     CDS             complement(5737..6273)
                     /locus_tag="VIH70_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8116273.1"
                     /translation="MRVDTEFEPLVTERLRLRRSRPEDAETISAYRSDPDVNRQQGWD
                     RTDPAGVLADIVEMSTRIPGEPGGWVQFTVEERGGGGIVGDVGFAVADGEPNVIKVGY
                     TISPEFQGRGYATEAIRALVNYSFDALGAEIVRAHASADNAASIRVAEKVGMRLVERV
                     EYREGAEVWHGVRYEVRR"
     gene            complement(6385..>7176)
                     /locus_tag="VIH70_07660"
     CDS             complement(6385..>7176)
                     /locus_tag="VIH70_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY8116274.1"
                     /translation="GFLGSMPGYLPGTLEAKLVAVFPGNHDRGLPSHQALIALFDEET
                     GAPLCLMDGTVITEVRTGATAAVAARALARQGASSLAVLGAGVQGRSHLDAFPRLFEL
                     DDIRVASRDPRHAETLAGSHPNARAVDSFEEAVRGADLVCCCTDADEPVLSRAWLAPG
                     AHVSSVGVGAEVDEPTVRASSIFVEWRGAAENPPPTGSAELQGLDPASIAEIGEVLAG
                     AHPGRHSDDELTLYKSTGHAVEDAAAARQVFDRASAEGVGATFEL"
BASE COUNT         1057 a         2596 c         2461 g         1062 t
ORIGIN      
        1 cgcggcgtcg gcgtcctcgc gtgtctccca caggctgagc gagacgcagg tcttgtcgcc
       61 ggagtcggcc aggccgtacc ggatgaaacc gggctggttc cggaactcgc gcagcatccc
      121 gtcctgtgcg acgtcggcga gctcctggaa cgagcccttc ttgatctcat acgtagcgat
      181 gcgaacgtgc ttcatgcgtg ttctcctttg ctcgtgtgcc cctgaccaac cagggccaac
      241 gtgagcgaga tccgagcgga tacgctcggg catcaggcct tcgctggagc cagcgagatg
      301 ggaccggatc ggtcgagacg atccccgtgc gtcctcttct cccgacaggt acgggggtcg
      361 cgtcttcgtg gagtgtacgt ctttgccacc tacccacgac cgcgaaactt gcgatcggga
      421 acccgaacgt cgtccgagcg cgcgcactcc gacgtgagat gatgatggca tcactcacag
      481 ggaccgtttg cgtcgaggcc gtgagcagcc cctccgatcg aggaggcacg atgaccaccc
      541 tcacggatcc ggtccacgtc gacgttccgg aacgagccgc ggtccaacct gcggctccga
      601 tcgatcctcc acgcgaaacg gaaccgccgg acacgaccgc cgaacggcgg gcggggtgat
      661 gcggccccga gaccccgaag cgacgacgaa cttcttccga accgagctcg gcaagacgct
      721 gcgtgtcgac gagaccgacg agggagcgat cagcgtcgac atcctcaagg aaggcgtgtg
      781 gtccgaggcg ccgcgcggga tgatcgggct ccggttgtcg ccgggcagcc gggggctgac
      841 cgccaccgag atcctcgcgc tcccgaccta gaggctcgat cgacagggac cgggcagacc
      901 gtccgcccgc gccggtgtcg gccgtcagcg ctcggagagc gcggccctta cgtcggggag
      961 agcgaggaag ctcgcgcgga gctcctcgtc gtcgatcgtc aggctgatcg cggagacggt
     1021 cgacgcggcc tcggcccgca gggctgcagc ctcgccgacg tcgccacgat cggccgcgac
     1081 accgctcagc tcgaggtcga tctcccacag caggcgtagg aagcccatgt cctcggcgcg
     1141 ggaccttgcc tcccgaagca gggggtcggc ctcccccggg cgttcggaag cgatgagctc
     1201 ccgcgatttg agcaggagtg cagcagggag gaactggcgc agctcgtgcc gatgcagcca
     1261 cgcgatcacc tcgtcggcga tctccacggc ctcgacgtgg tttccccgtg ccgccgcaag
     1321 ctgccctcgg aggatgtcga cgtgcgcgcc cgccgcgccc cggaccgggc cgggcagccg
     1381 cccgaggtcg gcctcggcga tcaaggactc cgcctgctcg gactcgcccg cgaggaacag
     1441 gatctcggcc ttcgacacca tcgccatggg gatggcgagg ggcgtctgac ccttcgcgat
     1501 ctcgagtccc cggtccgcga gttcgatccc ccgctcgaac tgccccagcc gcgcgtactg
     1561 gacccccaga tcggcgcgcg ccgccgactg cgggatcagg aaccccgatt cctcggagcg
     1621 ctggatgcac tcacgcatcc tggccatggc cctgcctacg ttgccctgtt ccacgtccac
     1681 caggtacgcg ttgaacaggg cgtacgcctg cccccacggg ttgccgatct cctcggagat
     1741 cgcgtaggcc tcgtcgtaga gcgccagggc ctcctccggc tcgccggcga gcgccagcag
     1801 cgccgcggtg ccgatgaggt tctcgcacag catggggagg ttgccgatct cgcgccagat
     1861 cggcctcgcc tcctccaggc aggagcgggc cgcgtcgacg tcgccgacgg ccgcgtacgg
     1921 gcgccacagg tcgttcaggg tgaaggcgat ctgctccctt gcgttcagct ctcgtgcgat
     1981 ctccagcgac cgctcccccg cctcgacggc ctcgtcggca tcgccgccgc cgaagacgtt
     2041 caggatcatc aggttccaca gggccttcga ctccgccgag cggtcgccga gctcccgcgc
     2101 gagcgcgatc gtgcgctcgg ccagcacccg ccccttggag gagtcgaaca ccggcgtcgg
     2161 cgtcgcgtgg atcgtggtga gggccatctc cgcccccagc acgccgccgg ggttacccgc
     2221 ctcccgcgcg agcgtcgcca tctcctcgta ggtcgcgacc gcctcgtcgt agcgaccgga
     2281 gagctcgagc acccggccgc gactcggatg caggtggccg atggaatcgg ccgtcgcccc
     2341 gcgccgcttg gccgcatcga tcgccgtcgc gtactgggtg gccgcctcgg cgttcgcgta
     2401 cagccggccc gcgttgtcgc cggcgatcgt cgcgtaccgc agcgtccgct catcatcccc
     2461 cgcctcccgg aagtggtagg cgagcaccgg cgcgaactcg tcgagccggt tggcgtagcg
     2521 ctcctcgaag acctcaccca cgcgcaggtg cagctcgcga cggcggcgga gcaggatcgt
     2581 gccgtaggtc gcgtcctgcg tgaggctgtg gtggaacgca tacccgcggt cgggggtccg
     2641 ggcggtctcg gagatcagac ccacccgctc gagcgttcgg agcgaggagg tgaggtcgtc
     2701 ctcacccgcc acggctcgaa gaaccggccc ggagaacgag cggccgatca ccgaggcgat
     2761 ctgcagcaga tgcttggcct gctcgtcgag cgcgtcgagt cgcgcggtga acaacgcctg
     2821 gagcgtggtc gggatcgaga tgtcgtcgtg gtgttcgagg agggcggcgg cgacctcctc
     2881 cacgaaaagc gggttgccgt cggtgcgctc gaggatctgc gcacggacgg ccgggtcctc
     2941 gtccgggatc agcgcgtcga tcaggcggcc gctctcgtcg tcggagagtg ggtcgagcgc
     3001 gagctcggtc gaccggtgcg ggtactcggt ctcgagccac tgcttgaggc gccacgacgg
     3061 cgcctgccgg tcgggccggt aggcgaacag gaacaggcag ggcagctcgt ccacgagctt
     3121 ggccgtctcg atcaacacgt ccatcgaggc ctcgtcgcac cagtgcaggt cctcgaagac
     3181 cagcagacgc ggctcgtcgg cgccgaaggc acgcgtcgcc cccggcacca gctccatgat
     3241 cgcgtcccgg aacgcctgtc cctccagtgg ctcctccccc ggctcggtga cgccgaacag
     3301 cgaccgccac acgcgcatgt ggggttcgag ccattcctcc tccgaaccgg gctccatcgt
     3361 cgcggccagc ttccggcgga ccacgtcggg ggggtcggtg tcctcgatcg cggcgagccg
     3421 cgccagcatg cgccggtatt gcgcgtatgg gcgtgtcgcg tcatacgaga cgcactgcca
     3481 ggtctcccag aggcggtgga tctcgcggcc gacgcgcgag gaatcctcgg gccgccggcg
     3541 ggtccactcg gtaaccgttt cctggacaag ccgggacttg ccgagccccg cctcgccgat
     3601 cagcgagacg atcctcccgc cgcccgactg cgcctcatcg atcgcgtccc ggagccactc
     3661 catctcgcgt tcgcgcccga ccagcggcga tccgcggagg ctccgggcct ccgtcacccc
     3721 ggccttcctc ccgaggaccc ggaacaccga cacgggctcg gccttgccct tgacctcgat
     3781 cccgccgcgc gattcgaggt cgaagaggga atcgaccagc cggtgggtct ccgccgtgat
     3841 ctgcacggtg ccgggctcgg ccgtctgctc catgcgcgcg gccacgttga ccgcgtcgcc
     3901 catcgccgtg tactcgacgc ggaggtccga gcccacctgc ccgaccatca ccgggcccgt
     3961 gttgatgccg acccgaacgt cgagatccag cccacgctcg gcgctgatcc gctccttgta
     4021 ctgggcgatg ccggcgatga tgtcgagccc cgcctggacc gcgcgctgag ggtcgtcctc
     4081 gtgcgcgatc ggcgcgccga agaacgcgaa gatcgcgtcg cccatcaggc gggcgagcgt
     4141 gccctcgtac cggtagacgg gggcgatcag ccgttcgaac gccccgttca tgatctccgc
     4201 ccagtcctca gggtccaggg tctcggccat cgcggtcgaa cccttgacgt cgcagaacag
     4261 catggtgacg atccgccgct caccctccat ggcccgcccc gcgcgtgcgg cctcgagctt
     4321 ggcgagcagc tccttgggca ggtagggctc gaggtgggta tcggggtcga ccgcggtgga
     4381 gtcgtggagc ttcgcgccgc agttcaggca gaagcgggcc tccgccggat ttgcggccga
     4441 gcagtcgggg caggtccttg gcgtcggcat gggattcggg cgtggtctgg gtggtaacga
     4501 tagccccgtt gcacatcggc cctggctcgc gtgccggtgt gcgtaggggt gggcgatccc
     4561 gcccgagcca ccgtcaggct ggccgatcgc ccgggaccga ccgcggccct cgctacgacg
     4621 cgagccgcgc cgtgacgctc atctccacgt tgcccttgag cgcgctcgag accgggcagt
     4681 tctccttcgc gtcctcggcg agggtgtcga agtcctcttc cgagatcccc ggcacgacgc
     4741 cggtcacgtc gagcgccgat gtcgtgatcc cgctccccgg cacgaacgtg actgcggccg
     4801 agacctcgag ccggtccgga ggcgtcccag ccttcgcgag tcgcgccgag aacgccatcg
     4861 agaagcatga tgcgtgggcc gccgcgatca gctcctcggg actggtcatc ccgctctcgt
     4921 cgccggtgcg ggccgcccac gagaccgcga gcgccccgat cgccccgctg cccacctgct
     4981 cgatcgtgcc ggtgccgttc tccaggtcgc ccttccaact cacccgtgcc tgtttggtcg
     5041 ccatcgactc ctcctttccc tccagctaat cgcgaccgag ccgctcaggc aaccccaggc
     5101 gtgaagtcct gcgggagtgg gtagctcgac gctagaaggg ggatggcaca aggcctcagc
     5161 agggaggagg atgtcatgcc gcagagagcg tggagcgcca agcgcgaacg acagtacgag
     5221 cacatcaaac agggtctcga ggagcgaggt cgcgacgagg acacggcgga ggagatcgcc
     5281 gctcgaacgg tcaacaagga gcgggcccgc tccggcgagg ccaggacctc gagcccgacc
     5341 tcgacccggg acgtctcgtc cggccggcgc ggtggcctgc ggtcgcaccg cgggcccggc
     5401 ggccgcacca aggcgcagct gtaccgggag gcgagggaca agcacgtcga gggacgatcg
     5461 aagatgacga aggcgcagct ccagcgtgcc gtcgaccgcg actgaggagg cggactcgtg
     5521 ccgagtgtcc aggggctctc caagacgcag gcgaactacc gcaaggccga gaacccgcag
     5581 ttccgctgcg gggagtgcaa gttcatgttc ccgcgcctgt cgatcggcgg gtgccgctac
     5641 gttcgcggcg tgatccacaa cgacgacacc tgcgacgagt tcaagccgcg cgacgccggc
     5701 tcgtcctgac ggtcgttcgg gacgtctgct cgagcctcag cgacggacct cgtagcgcac
     5761 cccgtgccag acctcggcgc cctcgcggta ctcgacgcgt tcgacgagcc gcatgccgac
     5821 cttctccgcg acgcggatcg aggcggcgtt gtccgcactg gcgtgggcgc ggacgatctc
     5881 ggcgccgagc gcgtcgaagg aatagttcac gagcgcgcgg atggcctcgg tcgcgtaccc
     5941 gcgcccctgg aattcgggcg agatcgtgta ccccaccttg atcacgttcg gttcgccgtc
     6001 ggcgacggcg aacccgacgt cgccaacgat cccgccgccc ccgcgctcct cgaccgtgaa
     6061 ctgcacccag ccgccgggct ccccggggat acgcgtgctc atctcgacga tgtcggccag
     6121 gacgccggcg ggatcggtgc ggtcccaccc ctgctgccgg ttcacgtccg ggtcgctgcg
     6181 gtacgccgag atcgtctcgg catcctccgg cctcgaccgg cggagccgga ggcgttcggt
     6241 cacgagcggt tcgaactcgg tgtcgacgcg cacggccgca gcgtagcgtt cgacgtcggg
     6301 cgccgccggt gaggatcgtg gccagcttcg aggcgtcgat gcttcctccc gacacgatcg
     6361 cgacgaccct gtggatcggc tccgtcaaag ctcgaacgtc gccccgacgc cctcggccga
     6421 ggcccggtcg aagacctgcc gagccgccgc cgcgtcctcc acggcgtggc cggtcgactt
     6481 gtagagcgtc agctcgtcgt ccgaatggcg tccggggtgc gcgccggcga gcacctcccc
     6541 gatctctgcg atcgaggccg gatccagccc ctggagctcg gccgagcccg tgggcggcgg
     6601 attctcggcc gcgccgcgcc actcgacgaa gatcgacgac gcgcggaccg tgggctcgtc
     6661 cacctcagcg cccacgccga ccgaactcac gtgcgccccc ggcgcgagcc acgcgcgcga
     6721 caggaccggc tcgtcggcat cggtgcagca gcacacgagg tcggcgcccc ggacggcctc
     6781 ctcgaacgag tcgaccgctc gcgcattcgg gtgcgacccg gcgagcgtct cggcgtggcg
     6841 cgggtcgcgc gacgcgacgc ggatgtcgtc gagctcgaac aaccgcggga acgcgtccag
     6901 gtgcgaccgc ccctgcacgc cggcgccgag gaccgcgagc gagctcgcgc cctggcgggc
     6961 gagcgctcgc gcggccaccg cggcggtcgc gccggtccgg acctcggtga tgaccgtccc
     7021 gtccatcagg cagagcggcg ccccggtctc ctcgtcgaac agcgcgatca gcgcctgatg
     7081 agacgggagc ccccggtcgt ggttgccggg gaagaccgcg acgagcttgg cctcgagcgt
     7141 ccccggcagg taccccggca tcgagccgag gaagcc
//
LOCUS       DATBRK010000252         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2351275, whole genome shotgun sequence.
ACCESSION   DATBRK010000252 DATBRK010000000
VERSION     DATBRK010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2351275"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1698
                     /locus_tag="VIH70_07665"
     CDS             226..1698
                     /locus_tag="VIH70_07665"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEY8116275.1"
                     /translation="MKKTAIVFALVAGLGIGLVGSALAGLDFGLDEQNLLNSKPLPLF
                     GVTKPIAASSKASISAATAEADPTALVTLANGLSARVVTAAANAGPNIDMMALWPNDT
                     NPTWLIACNEQGAAQPALQRIRISDGLVETIVASGLVSCDPAHRTPWGTIVFAEENGT
                     SGRLFELIDPLTTTGVAISAATTTPATVTCPPSPPACEGTGNIVERTAVGRLSFEGIG
                     ITPNGVMYYGDENRPLNGTPGGAYFKFIPACAPTATCPVGSLPGANNEAKLAASPFAS
                     AGTIYGLRLGKRSGNTDYGQGTQTGRGTWIPEAGAAPINLRGAVASQFLTGYYRPEDL
                     EFDFEAIANGDVRFCANNTGNEGDDRNYGETICVTDGTLAQAANTATLSIPEVQYSVL
                     GNPQFAMMDNIAYQPGRGNWVIHEDGDQLQGNNDLFDCLPDGADDNLLSDGCVRIGTE
                     NDLSAEWTGGVFDATGTRFFVSVQHNVTEHGVVLEITGWK"
     gene            complement(1764..2426)
                     /locus_tag="VIH70_07670"
     CDS             complement(1764..2426)
                     /locus_tag="VIH70_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEY8116276.1"
                     /translation="MTRRYGQYCGLAKALELVGGRWSLLIVRELLTGPKRYTELQHGL
                     PGIPTNILSSRLRELEEAGLVERSLLAHSSSVVYGLTAYGLDLEEAVVRLGVWGSKSL
                     GRPGEDDQFSLSALALALRGAFDPSKATGPDLLLEIRLGDEPPLVVMVSEGRVSFPTA
                     PPSRPIVLETAPEVVSELLVGSLDLSAAVASGRARVDGSIREATRFFDLFRLPAREPV
                     TG"
     gene            2574..>3664
                     /locus_tag="VIH70_07675"
     CDS             2574..>3664
                     /locus_tag="VIH70_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8116277.1"
                     /translation="METTPSRKSLILALACTAQFMVVLDIAIVNVALPAIQRELHMDQ
                     STLQWVVIAYGLLLGGFLLLGGRLGDLLGRRRVFLTGLALFSGASLLAGLAQSQELLI
                     GARALQGFGAALVAPTALSILAVTFSEGRERNWALGIFGAVAGSSASIGVIASGLLTD
                     GPGWRWVFFINVPIGIALIALAARFLPADRPEVSSRRFDALGATAVTGGLLLLVYGLN
                     RGVDHGWTSASTLALFAGALLLLATFVRVESRSGSPLVPGALLRNRTMVASDIAAFLL
                     FGSFFSFIFLATLLMQQLLQYSPTRTGVAWLATSVTAFLAAGIAGARLVSTIGVRRLL
                     IAGMSLLALGLLWLVRVPPGASYVTDLLPG"
BASE COUNT          568 a         1270 c         1256 g          570 t
ORIGIN      
        1 aagggcgtcg aggaggtccg cgcgatccgc gaccagatcg acgatcgcgt gaaggcgctg
       61 ctcgccgagc tcgtgcccgc gtcggcctga tgccccggcg gcgaggcgcc gagaggcgtt
      121 cacgggagtt catcgcccga acaccttccg gccaactccc cccactagaa ctgggcccgc
      181 accggctagg tgcgaccgca gggtcgcgag aggacggagg ggcagatgaa gaagacggcg
      241 atcgtattcg cactggtcgc agggctcggg atcgggctgg tgggctccgc actcgccggc
      301 ctcgatttcg ggctggacga acagaacctg ctgaactcga agccgcttcc gctgttcggc
      361 gtcaccaagc cgatcgccgc ctcgtcgaag gcgtcgatct ctgcggcgac cgccgaggcc
      421 gacccgacgg cgctcgtgac gctcgcgaac ggcctgagtg cgagggtcgt cacggccgca
      481 gccaacgccg ggccgaacat cgacatgatg gccctgtggc cgaatgacac caacccgacc
      541 tggctcatcg cgtgtaacga acaaggagcc gcccagccgg cattgcagcg gatccggatc
      601 tcggacgggc tcgtcgagac gatcgtcgcg tccggtctcg tcagctgcga cccggcgcac
      661 aggactccgt gggggacgat cgtgttcgcc gaggagaacg ggacgagcgg gcggctgttc
      721 gagctgatcg acccgctcac caccacgggt gtggcgatat cggcggcgac gacgaccccg
      781 gccaccgtga cgtgcccccc ctctccaccg gcgtgtgaag gcacgggcaa catcgtcgag
      841 cggacggccg tcggccgcct ctcgttcgaa gggatcggca tcaccccgaa cggggtcatg
      901 tactacggcg acgagaaccg cccgttgaac ggcacacccg gaggcgcgta cttcaagttc
      961 atcccggcct gtgcaccgac cgcaacgtgt ccggtggggt ccctcccggg cgcgaacaac
     1021 gaggcgaagc tcgccgcgag cccgttcgcc tccgcgggca ccatctatgg gctgcgtctt
     1081 ggcaagcgct ctgggaacac cgactacggt cagggaaccc agaccggacg gggtacctgg
     1141 atcccggagg ccggggcagc ccccatcaac cttcggggcg ccgtcgcgag ccagttcctg
     1201 accgggtact accgaccgga ggacctcgaa ttcgacttcg aggcgatcgc caacggcgac
     1261 gtccgtttct gcgccaacaa caccgggaac gagggcgacg atcggaacta cggcgagacg
     1321 atctgcgtga cggatgggac gctcgcgcag gccgccaata cggccacgct gtcgatcccc
     1381 gaagtccagt actcggttct cggcaatccg cagttcgcaa tgatggacaa catcgcctac
     1441 cagccgggcc gcgggaactg ggtcatccac gaggacggcg accagcttca gggcaacaac
     1501 gatctgttcg actgccttcc cgacggtgcg gacgacaacc tgctctccga tgggtgcgtg
     1561 cggatcggga cggagaacga tctgagcgcc gagtggacgg gcggggtctt cgacgccaca
     1621 ggcacccggt tcttcgtcag cgttcagcac aacgtgacgg agcacggcgt cgtgctcgag
     1681 atcaccggct ggaagtagtc gcgccgggaa cgcgaacggc agggccgaga ccccgggcga
     1741 tcgcccgggg tctcgcgcgc cgatcagccc gtgacgggct ctcgggcagg caggcggaag
     1801 agatcgaaga accgcgtggc ctcccggatc gagccgtcga cccgggctcg tcccgacgcc
     1861 accgccgcgc tgaggtcgag ggatccgacc aggagctcgg acaccacctc gggagcggtc
     1921 tccaggacga tcggcctcga gggcggcgcg gtcgggaacg agacccggcc ctcgctcacc
     1981 atcacgacca gcggcggctc gtcgcccaaa cggatctcga gcaggaggtc ggggcccgtc
     2041 gccttggacg ggtcgaacgc gccgcgaagc gccaacgcca gcgcgctcag gctgaactgg
     2101 tcgtcctcgc ccggcctacc gagcgacttg gagccccaca cccccaggcg aacgaccgcc
     2161 tcctcgaggt cgaggccgta ggccgtcagc ccgtagacca cggagctcga atgggccagg
     2221 agcgagcgct ccacgaggcc cgcctcctcg agctcgcgca ggcgggacga caggatgttg
     2281 gtcgggatcc cggggagccc gtgctggagc tcggtgtagc gcttggggcc ggtcaggagc
     2341 tcgcggacga tcaggagcga ccagcgcccc ccgaccagct cgagcgcctt ggcgaggccg
     2401 cagtactgcc cgtacctccg tgtcatgtcc ggaagtatcc cacgacccct tgacagactc
     2461 aaggagactt gctaaacaca agtttccttt cgacagagga gcaagtgtcg atttccgccg
     2521 tgcccgttcg tcgttcgtgg agacgtcgaa ccccgagatc ccgaggagga gtcatggaaa
     2581 ccaccccttc caggaagagc ctgatcctcg ccctggcctg cacggcgcag ttcatggtgg
     2641 tcctcgacat cgcgatcgtg aacgttgccc tgcccgcgat ccagcgggaa ctccacatgg
     2701 accagagcac gctgcagtgg gtggtgatcg cgtacggcct actcctgggt ggcttcctgc
     2761 tcctgggcgg ccggctgggc gacctgctcg ggaggcgccg ggtcttcctg acggggctcg
     2821 cattgttttc cggcgcctcg ctcctggccg gcctggccca gtcgcaggag ctgctgatcg
     2881 gggcgcgtgc gctccagggg ttcggggcag ccctcgtggc gccgacggcg ctctcgatcc
     2941 tggccgtgac cttctccgag ggacgggagc ggaactgggc cctcgggatc ttcggagcgg
     3001 tggccgggtc ctcggcctcg atcggggtga tcgccagcgg gttgctgacc gacgggcccg
     3061 gatggcggtg ggtcttcttc atcaacgttc ccatcgggat cgccctcatc gcgctggcgg
     3121 cgcgcttcct gcctgccgac cgtcccgagg tgagctcgcg ccggttcgac gcgctcggcg
     3181 cgaccgcggt gacgggtggt ctcctgctgc tcgtgtacgg gctcaaccgg ggcgtggacc
     3241 acggctggac gtccgcgagc accctcgcgc tgttcgccgg ggcgctcctg ctgctggcga
     3301 ccttcgtccg ggtcgagtcg cggtccgggt cgcccctggt ccccggggcc ttgctccgga
     3361 accggaccat ggtggcctcg gacatcgccg ccttcctgct gttcggctcg ttcttctcgt
     3421 tcatcttcct ggcgacgctg ctgatgcagc agctcctcca gtactccccc acccggacgg
     3481 gtgtggcgtg gctcgccacg tccgtcaccg ccttcctcgc ggcggggatc gcgggggctc
     3541 ggctggtctc cacgatcggg gtccgacgtc tgctcatcgc cgggatgtcg ctcctcgcgc
     3601 tcggtctgct ctggctggtt cgcgtcccgc cgggcgcgag ctacgtcacc gacctgctcc
     3661 cggg
//
LOCUS       DATBRK010000253        13869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_621204, whole genome shotgun sequence.
ACCESSION   DATBRK010000253 DATBRK010000000
VERSION     DATBRK010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621204"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..687)
                     /locus_tag="VIH70_07680"
     CDS             complement(118..687)
                     /locus_tag="VIH70_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8116278.1"
                     /translation="MRDVTYSMGVSLDGFIVGPDGEFGWTAPDAEVFRFWIDEIRGVG
                     VHLLGRRLYETMLYWETADQDPSLDDAELEWAALWKPLPKVVFSTTLSAVQGNARLAS
                     GGLAEEIERLRAERGEGDIAIGGATLAAEAAAFGLIDEYRAMVYPVLVGGGIPFFPRR
                     ERRVDLELVETRTFSSSVVYLRYRVARQP"
     gene            complement(796..1899)
                     /locus_tag="VIH70_07685"
     CDS             complement(796..1899)
                     /locus_tag="VIH70_07685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8116279.1"
                     /translation="MSEQVARRVRAAVEHHGPIGFDEYMEIALYGPGGFFEAPPIGAD
                     RHFVTSPHVHPFVFAHCVRDALLDAWFGLGEIEPFPLVELGAGNGTLADALLTAFAEL
                     PAPRPAYTGVEISPGARDALAARGLATASAVSDLDPFDGVIVANEVLDNLAFVPVRAR
                     PEGPKEVRVGLRDRSLVEVEVPWPNDSVRPPPLEAGEETTVPVGAFALLEMLVPVLRR
                     GYVLLIDYGSVEGPAGPVHGYRDHREVADVLADPGGADITAGVDVSMVVERARSVGLR
                     AFDPVSQRAALRALGHDRWQRTMRTTQSALQRAGLGAEAVRVWEERSRASLLADPSGF
                     GGFWWLVLATEGLPEPPWLTRARTGAGRRGGDS"
     gene            1981..2616
                     /locus_tag="VIH70_07690"
     CDS             1981..2616
                     /locus_tag="VIH70_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8116280.1"
                     /translation="MDEGIREAASAVVARDGEQGLEVVVLERGAASRFLPGYVAFPGG
                     STDGQDGELARRWFGTPEEARRACAVRELVEEVSLVLTRTGVAQGGELGAIDADPPSA
                     GQLPELAHWVAPPRVPVRFDARYFAVRAPVGIEPVPDGGETADAWWVSPARLLEDWTA
                     GRRRLYWPTFFTVTEISGCRSVDELFGLRIRTREPDDAELERLPRSTFWQE"
     gene            2622..3479
                     /locus_tag="VIH70_07695"
     CDS             2622..3479
                     /locus_tag="VIH70_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8116281.1"
                     /translation="MRVIRVLAPNPGIRELEGTNTWIVGEAPAIVVDPGPDDPGHLVE
                     VARTAGSIGAIALTHDHPDHAPGAVPLGAMTGAPVFAARPAEGMDRIRDGEQVSAGTL
                     VLSVVGTPGHTPDHVAFFDARTGSLFTGDAVLGRGTSVIDPPEGDLAAYLRSLRRMRG
                     LAPKTIYPGHGPVVLRALAKLDEYLDHRAMREGQVLSALGDAWRTPEELAAEIYADYL
                     PEVHELAARSVLAHLIKLEVEGRAEKRTKAEVVRWSAIEPRSCERCGRPVRGRVRLCG
                     SCTVAVLQE"
     gene            complement(3501..4130)
                     /locus_tag="VIH70_07700"
     CDS             complement(3501..4130)
                     /locus_tag="VIH70_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8116282.1"
                     /translation="MGVALDRTEGGGTRRLPHGEVIVRQGDTVSSLFLVTAGAVRLSS
                     VTASGREIVVGLLCRGDLFGESALLGDPSLVRAQAVGPTTLLALPIPSLRSTLERTPA
                     TAEELLRLIAARLHRTSAALEDAMAADLPSRVVGRLRELADDHGVPGPNGVRLRVPLT
                     QDELARMVGASRESVNRTVGALAARGVVRSGGGRIVITDPDALRNASNR"
     gene            4257..4679
                     /locus_tag="VIH70_07705"
     CDS             4257..4679
                     /locus_tag="VIH70_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8116283.1"
                     /translation="MAVRARDALAQVPLFAGMPSRFLKRLGDKMDEQRFMEGATIVRQ
                     GEPGDTFYVIVEGEAKVKDRNGRTLSGLIPGDFFGEISLMDGGPRTATVVAETDLTAL
                     ALSRKDFGALLQSEPKVTVGLLKHSATLLRRLERPASG"
     gene            4682..5035
                     /locus_tag="VIH70_07710"
     CDS             4682..5035
                     /locus_tag="VIH70_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116284.1"
                     /translation="MRAELYRPDAPESVVAVATWSDGRANLEVLDASIEHIGTLIRPT
                     PVVVDDPSLRPPGTHGDALLEPGSSAWFRAALRTRAEPLGLRVRFVAGKIVGGWDPAA
                     SSGSFDEQVDRLSSS"
     gene            complement(5036..8308)
                     /locus_tag="VIH70_07715"
     CDS             complement(5036..8308)
                     /locus_tag="VIH70_07715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY8116285.1"
                     /translation="MTERYPRPIVDFLRGEPTPEQWTAISWPLEPCVVVAGAGSGKTS
                     VMAARVIYLAMVATGAWPADHEGVMPGNALCLTFTNKATENLILKMRRALRTLDLDEG
                     EEPEISNYHAFAAQVLERHGMLIGLEPGQRVLSQAQRAQIAARVLDRMTFEKLHTRWQ
                     PAIVGYILGLDEQLQNHLVEPETVIEHVEGKLEALRKARSAEPLLAAEERIEVARAVR
                     VFRELKAELGVIDFGDQIAHAVRIASAHPDVGRAYRDRFGAVLLDEYQDTNHAQAVLM
                     RAVFGDGHPVTAVGDPDQNIYAWRGASLWNLLHFRKQFPLADGAESAQLPLYTNFRSG
                     ARILQAADRVIDALPTNQRPDPDKLLRPWERNGEGHVEVARYEHEVAEAEGVAERARE
                     LHADGVAWREMAVLCRTHRLFEALQLAFGAMEVPAEFVNLAGLINLPEVVEVLAYARA
                     TEDPRDGVALARILTGPRYRVGLRDLGVLAGWSRTSGGELRQLLADLALDEDDDLLED
                     HPFLMAEALERLDEIEGLTEEGRVRLAEFGGELDELRVAARRPVGEFLAEIIRRTGLL
                     AELDSAADQPMALARRRNLGAFLEQVHAFQPVDGELTLRAFLEHLDSIDDDREWNPVQ
                     PSDDDSVKVMTVHAAKGLEFEAVFVPGLANGLFPDARVQQNPARKGSSLDVELRRDRD
                     LLPSFDDNMKAFTDALREQEVAEERRTCYVALTRAKRHLFVSSANWYGENLMAKGVGR
                     FLVELLTWARRGGGTVWPADEPGAQDAESAQTNPLEGYRQRFVRPWPGPARPDDRDEL
                     FAEGWRRAALSAHERGRVAAALLETLSPDERMRFEESSAARRTLATHLRAQEAEVDQR
                     AARPSTVSVGGLIGYARCPKLYYWSAVRPLPRFAGPSARIGTEIHRWIEMKSRGQATL
                     LEVEDEPDVTVEELAGTPGKLEDLRSAFLRSRFADSVPLYAERPFLLPLEGIAIKGRI
                     DAIYGLPDGPWEVVDYKTGRLPPDDDPLARLQLDLYALACIEVWHKAPEDLRLTYLYL
                     AAGEERSHGVGDVDAVRQRIGSWLRGIGEAAFEPTPGPQCRFCDFRPFCDAGKAWVAS
                     NQ"
     gene            complement(8305..11211)
                     /locus_tag="VIH70_07720"
     CDS             complement(8305..11211)
                     /locus_tag="VIH70_07720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY8116286.1"
                     /translation="MAPFEPDPRQLEVLDHADGAMLVSGGSGTGKTAVLRERFARLVE
                     RGADPERVALVVGSRRARDEARRLLLERLPVALPSLTVVTIHGLAFHVVGERFERLGY
                     DAPPTVLSASEQFARVRELLDGEDAGRWPVFGPLLGLRGFADEIRQLVIRAQEALRSP
                     DDIERRAAERGLAGWDELAAFLRHYLSVLDASSEVDFAGLVEQAAVAAGAGDPPFDHV
                     MVDDYQDTTFAAERLLAEIRPRTFVAVGDLEAHLFSFQGTTDEPLRRFAERNPGAATV
                     LLETRHRPEPDPVEAWRAGHVSEQHAAVARELRRLHVDDQVPWRELAVVVRRHGPRVE
                     GLLRALDDAHVPRRIADAGSPAVVPATRPFVLALKWLVAGPQERDSLIEPVLTSELGR
                     LSPASVRALLRLARAHGRPPRDALAMHDLAEPEDAEALTALGGVLARAEAVAASVLDA
                     FRVLWLELPFAADLVERAETDHHAFVDLEAVVELSNAVASAGTAADPSTEAFLLSLGA
                     AEGAPILAAGDEGSHDEVHVLTAHAAAGREFDTVVVLDALEGDFPSLSRPEPMFDLAT
                     LDEAIARSEVNRRRLADERRLFGMVRSRARRRVVLTATDPHGDESSVTLTSRFVEELG
                     LPWRDVPSAPFAEPVSTFEAASAWRRGLGDRTAAPAERLACLDALLALGDDPGRWWFQ
                     RDWTDLSAMAEDALSLSYSRLSTLENCELQFVLSSELGLDPGGGYQAWVGKTVHRIIE
                     ECERGSIGRTPEAFERALDERWEKTRFPSYAVSEAERANARTVLIRNWFERYAEPPAT
                     ATERGFAFEFEGARIRGKIDRIGPVPEGGTRITDYKTGRSDSAPRPAESLQLGIYYLA
                     VHEDDDLAEHRPVQAVELAFLGGRKADPALDVREWSVSEDIEEEYKARMRERLGELID
                     RIRELNEERSYIANTKASCFFCRFQTLCTRYPQGDAVFPIEGLVTAPAEVVAGP"
     gene            complement(11262..12359)
                     /locus_tag="VIH70_07725"
     CDS             complement(11262..12359)
                     /locus_tag="VIH70_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY8116287.1"
                     /translation="MFGRGFRIATIRGVPVNVDSSWIWIAVLLVATFWARLDQEFPEI
                     ASGEAFAYALFGALVFFGSVFLHEAAHAIAARANGIEVHGITLVFFGGFTAARADAKG
                     PGPAFAIAALGPGTSLALSGTFWLLSRLTADIAGPLPGLFSYVATVNLFMAIFNALPG
                     LPLDGGRMLQSLVWRLTRDQQRATRVAARSGLLVGGIVIALAIFALTQERLFEAVWGG
                     IIGMFIFRGAQASEQRIGIDGRLASATVAEAMDPPPPAVPADLSLSQTLDRFLRGHEE
                     EAFPVIEDGTVIGMISFNSARELGIRDPLRPARDALIPLEHVLVARPEERLDEVSARL
                     GTDRAALVLRDGRLVGAISGSGLYRWAARAG"
     gene            12422..>13869
                     /locus_tag="VIH70_07730"
     CDS             12422..>13869
                     /locus_tag="VIH70_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8116288.1"
                     /translation="MDDGGHEPSERGPRHRLRAAATVLLACVVGLVGAWLGLALLARE
                     TVAIGPFRVKLDVGFGRGETVLGLPPFGALTADTHLSPLHVSATLQDVGVQRLTDVVN
                     DEGLDGLVSDVERDARGSVRTIAWRTLLAGTIGGIALAALVYRNHWHRVATGGVAALL
                     GVAFCEVLLLTTFQPAAFAEPSYSGSLALAPQLIGPVSEATGRIEDLRAGLEQIVDGT
                     VRAYTSLQASPPADDAIRVLHMSDIHASPLGMDFAQEVARGFGVDFVIDTGDITSFGT
                     PVENLIASEIPGFGVPYVFVRGSHDSIALQEAIAREPNAIVLDGREKTIDGLTVYGLG
                     HPAFTPARGAPVDAEDFAALARSAGPVIAADLEAAPEPVDVVAVHDDRMAEAVAGRVP
                     LVISGHFHETTSTVRNGTLYLRVGTTGGSGAGIFRDLADIPFSAEVLYFSRESDPRLI
                     AYDVIEQLPESGSLTVQRITVSVEFGDLVLTPT"
BASE COUNT         1934 a         5003 c         5032 g         1900 t
ORIGIN      
        1 gacctgatcg cgctcttggg ttcgatcttc ttcgtggtcg gcgacatcga ccgctagccg
       61 tggttcccac ggacgtcgtt gacacaggcg agagcctttc cgctccgatc cgagcgctca
      121 gggctggcgc gccacgcggt atcggaggta gacgacgctc gagctgaagg tacgggtctc
      181 gacgagttcg agatccaccc ggcgctcgcg ccgcggaaag aacgggatgc cgccgccgac
      241 cagcaccggg tagaccatgg cccggtactc gtcgatcaga ccgaacgcgg ccgcctcggc
      301 ggcgagcgtc gcgccgccga tcgcgatgtc gccctccccc cgctcggccc gcaagcgctc
      361 gatctcctcc gcgaggccgc cggaggccag gcgggcattg ccctggaccg ccgacaacgt
      421 ggtggagaac accaccttcg gcaatggctt ccagagcgcg gcccactcga gctcggcgtc
      481 gtcgagcgag ggatcctggt cggcggtctc ccagtacagc atcgtctcgt acagccgtcg
      541 ccccaagagg tggacgccga cccctcgtat ctcgtcgatc cagaagcgaa agacctcggc
      601 gtcgggcgcc gtccagccga actcgccgtc cggcccgacg atgaagccgt cgagtgagac
      661 gcccatcgaa taggtcacgt cgcgcatcag agatcctcct cggttacggg ctcgataacg
      721 ggtccgacag tacgaccgcc ggacgccgca ggactcatca cggctcgcgg gatctcctgg
      781 gctggctgta gttggctagc tatcgccccc ccggcgcccg gcgccggtac gcgctcgcgt
      841 gagccacggc ggctcgggca gcccctcggt cgcgaggacg agccaccaga acccgccgaa
      901 ccccgagggg tcggcgagca ggctcgcgcg gctgcgctcc tcccagacgc ggacggcctc
      961 ggcgccgagt cccgcccgct ggagcgccga ctgcgtcgtg cgcatcgtcc gctgccaccg
     1021 atcgtgcccg agggcccgca gggcggcccg ctgcgagacg ggatcgaatg cccgcaggcc
     1081 gaccgagcgc gcccgctcga cgaccatcga gacgtcgacg cccgccgtga tgtcggcgcc
     1141 accgggatcc gcgaggacgt cggcgacctc tcggtggtcg cggtacccgt ggaccggccc
     1201 ggccggtccc tccacgctgc cgtagtcgat cagcaggacg tagccgcgcc gaagcaccgg
     1261 caccagcatc tcgagcagcg cgaacgctcc gaccggcact gtcgtctcct cgcccgcctc
     1321 gagcggcggc ggccgaaccg agtcgttcgg ccacggaacc tcgacctcga cgagcgaccg
     1381 gtcgcgcagg ccgacgcgga cctccttcgg gccctcgggt cgcgcgcgga ccgggacgaa
     1441 cgccaggttg tcgaggacct cgttcgccac gatcacgccg tcgaacgggt ccaggtcgga
     1501 gaccgcggac gccgtcgcga gcccccgagc ggcgagcgcg tcccgcgcac cggggctgat
     1561 ctcgacgccc gtgtacgccg gtcgcggggc ggggagctcg gcgaacgcgg tcagcagggc
     1621 atcggcgagc gtgccgttcc cggcgccgag ctcgacgagc gggaacggct cgatctcgcc
     1681 gaggccgaac cacgcgtcga gcagcgcgtc gcggacgcag tgcgcgaaca cgaaggggtg
     1741 cacgtgtggg ctcgtcacga agtgccggtc cgcgccgatc ggcggggcct cgaagaatcc
     1801 gcccgggccg tacagggcga tctccatgta ctcgtcgaac ccgatcggtc cgtggtgctc
     1861 gaccgccgct cggacgcgtc gcgcgacctg ctcgctcatg ccgtcaagcc tagtgacccg
     1921 ctgctgggcc gacgcgggcg gtggatgtcg gcggtgcagg tgcatgcgag gatgccgctc
     1981 gtggatgagg ggatccggga ggcggcatcg gccgtggtgg cccgggacgg tgagcagggg
     2041 ctcgaggtcg tggtgctgga gcggggggct gcgagccggt tcctacccgg gtacgtggcg
     2101 ttccccgggg gctcgaccga cggccaggac ggggagctcg cccgccggtg gttcgggacg
     2161 ccggaggagg ctcgccgcgc gtgcgcggtg cgcgagctcg tcgaggaggt gtcgctcgtc
     2221 ctcacccgga cgggcgtcgc gcagggcggc gagctcggag cgatcgatgc cgatccgccc
     2281 tcggccggac agctgcccga gctcgcccac tgggtcgcgc cgccccgggt cccggtgcgg
     2341 ttcgacgcgc gatacttcgc cgtccgcgcg ccggtgggga tcgaacccgt gccggacggc
     2401 ggcgagacgg cggacgcctg gtgggtctcg cccgcccgcc tcctggagga ttggacggcc
     2461 ggtcgtcgcc ggctctactg gccgaccttc ttcacggtga ccgagatctc ggggtgccgg
     2521 tcggtcgacg agctgttcgg gctgcggatc cggacccggg agcccgacga cgccgagctc
     2581 gagcgattgc ctcgctcgac gttctggcag gagtgatcgc gatgcgtgtg atccgcgtcc
     2641 tggcgccgaa ccccgggatc cgcgagctcg agggaaccaa cacctggatc gtgggcgaag
     2701 cgccggcgat cgtggtcgac ccggggcccg acgacccggg gcacctggtc gaggtcgcgc
     2761 ggaccgcggg ctcgatcggc gcgatcgcgc tgacccacga ccacccggac cacgcgccgg
     2821 gcgcggtccc gctcggcgcg atgaccgggg ccccggtgtt cgcggctcgt ccggccgagg
     2881 ggatggatcg gatccgcgac ggcgagcagg tgtcggccgg cacgctcgtt ctctcggtgg
     2941 tcggcacgcc gggccacacg cccgatcacg tcgcgttctt cgatgcgcgc acgggctcgc
     3001 tgttcaccgg cgatgcggtg ctgggacggg ggaccagcgt gatcgatccg cccgagggcg
     3061 atctcgccgc ctacctgcgt tcgctgcgcc gcatgcgggg gctggcgccg aagacgatct
     3121 accccgggca cggtccggtc gtcctgcgcg cgctcgccaa gctcgacgag tacctcgacc
     3181 accgggcgat gcgcgagggg caggtcctgt cggcgctcgg cgacgcgtgg cgcacgccgg
     3241 aggagctcgc ggccgagatc tacgccgact acctacccga ggtgcacgaa ctcgccgcgc
     3301 gttcggtgct cgcccacctg atcaagctcg aggtcgaggg tcgagccgag aagcgcacga
     3361 aggccgaggt cgtccgttgg tccgcgatcg aaccgcgctc gtgcgaacgg tgcggtcgac
     3421 cggttcgcgg gcgggtgcgc ctgtgcggtt cgtgcacggt cgcggtgctc caggagtgag
     3481 agcgaccggg ggcggcgggg tcagcggttc gaggcgttcc gcagcgcgtc cggatcggtg
     3541 atcacgatcc gtcccccgcc cgagcggacc acgccacgcg ccgcgagcgc gcccaccgtc
     3601 cggttcacgg attcgcgcga cgcacccacc atccgcgcga gctcgtcctg cgtcagcggg
     3661 acgcggaggc ggaccccgtt gggaccgggg acgccgtgat cgtcggcgag ctcgcgcagg
     3721 cgaccgacca cgcggctcgg caggtcggcc gccatcgcgt cctccagggc ggccgaggtc
     3781 cggtgcaggc gggcggcgat caggcgcagg agctcctcgg ccgtcgccgg ggtgcgctcg
     3841 agggtcgacc ggagcgaggg gatcggcagg gcgagcagcg tggtcggtcc gacggcctgc
     3901 gcgcggacca gcgacggatc gccgaggagc gcggactcgc cgaacaggtc gcctcggcac
     3961 aacagcccga cgacgatctc gcgccccgac gcggtcaccg acgagagccg cacggcgccc
     4021 gcggtcacca ggaacaggct cgagacagtg tcgccttggc ggacgatgac ctcgccgtgg
     4081 gggagccggc gggtcccgcc gccctccgtc cggtcgagcg caacccccac gcggcgaccc
     4141 tagcgtgacc cgccgacagg cggcccgggc cggccacgga gtcttcgaca gcgcggctcc
     4201 gctcggagta ggctgcgctc ccgacgccgt cggacgacag gagaggggag ccgaaggtgg
     4261 ccgtacgggc tcgagacgcg ctcgcacagg tgccgctgtt cgccggcatg ccgtcgcggt
     4321 tcctgaagcg gctcggggac aagatggacg agcagcggtt catggagggg gcgaccatcg
     4381 ttcgccaggg ggagcccggc gacacgttct acgtgatcgt cgagggcgag gcgaaggtga
     4441 aggaccggaa cggccgcacg ctctccgggc tcatccccgg cgacttcttc ggcgagatct
     4501 cgttgatgga cggcggtccg cgcaccgcca ccgtcgtcgc ggagacggac ctcaccgcgc
     4561 tggcgctctc gcggaaggac ttcggggcgc tgctccaatc ggaaccgaag gtgacggtcg
     4621 gcctcctcaa gcactccgcc acgctgcttc gacggctcga acgccccgcc agcggctgag
     4681 cgtgcgcgcc gagctctatc gcccggacgc gccggagtcg gtcgtcgcgg tcgcgacctg
     4741 gtcggacggg cgcgcgaacc tcgaggtgct cgacgcgtcg atcgaacaca tcggcaccct
     4801 gattcgcccc acgcccgtcg tggtcgacga cccgtcgctc cgacctccgg ggacgcacgg
     4861 ggatgcgctg ctcgaacccg ggtcgtccgc ctggttccga gccgcactgc ggacgcgcgc
     4921 cgagcctctc ggtctgcgcg tccgtttcgt ggcagggaag atcgtcggcg ggtgggatcc
     4981 ggccgcctcg tccgggtcgt tcgacgagca ggtcgaccgg ctgagctcgt cgtagctact
     5041 ggttggacgc gacccaggcc ttgcccgcat cgcagaacgg gcggaagtcg cagaaccggc
     5101 actgcggccc cggcgtgggc tcgaacgcgg cctccccgat cccgcgcagc cacgatccga
     5161 tccgttggcg gacggcgtcg acgtcgccga ccccgtgcga acgctcctcg ccggccgcga
     5221 gatagaggta cgtgagccgc aggtcctcgg gcgccttgtg ccacacctcg atgcacgcga
     5281 gggcgtacag gtcgagctgc agtcgcgcga gcgggtcgtc gtcgggaggc agccgtccag
     5341 tcttgtagtc cacgacctcc cacggtccgt ccgggagccc gtagatcgcg tcgatgcggc
     5401 ccttgatcgc gatgccctcg agcgggagca ggaacgggcg ctccgcgtac aggggcacgc
     5461 tgtccgcgaa ccggctccgc aggaacgccg accgcaggtc ctcgagcttg ccgggggtcc
     5521 cggcgagctc ctcgacggtc acgtcgggtt cgtcttcgac ctccagcagc gtggcctggc
     5581 cgcgcgactt catctcgatc caccggtgga tctcggtgcc gatccgggcg ctcggtcccg
     5641 cgaaccgcgg cagcgggcgg accgcggacc agtagtagag cttcgggcac cgcgcgtagc
     5701 cgatcagccc gccgaccgag acggtggacg ggcgggccgc tcgttggtcc acctccgcct
     5761 cctgcgctcg cagatgcgtg gcaagcgtac ggcgggctgc ggacgactcc tcgaacctca
     5821 tccgttcgtc cggggacagg gtctcgagca acgccgccgc cacgcgcccg cgttcgtgcg
     5881 ccgagagcgc cgcgcggcgc cagccctcgg cgaacagctc atcgcgatcg tcggggcgcg
     5941 ccggcccggg ccacggccgg acgaaccgct gccggtaccc ctcgagcggg ttggtctggg
     6001 cggactcagc gtcctgcgcg ccgggctcgt cggccggcca caccgttccg ccgccccttc
     6061 gcgcccacgt gagcagctcg accaggaacc tcccgacccc cttggccatc aggttctcgc
     6121 cgtaccagtt ggccgacgag acgaacaggt gccgcttcgc gcgggtgagc gccacgtagc
     6181 aggtgcggcg ttcctctgcg acctcctgtt ctcgcagcgc gtcggtgaac gccttcatgt
     6241 tgtcgtcgaa gctcggcagc aggtcgcggt cgcgccggag ctcgacgtcg agcgagctcc
     6301 ccttccgcgc cgggttctgc tggacgcgcg cgtcggggaa caacccgttc gcgaggccgg
     6361 gcacgaacac ggcctcgaac tccagaccct tggccgcgtg cacggtcatc accttcaccg
     6421 agtcgtcgtc gctcggctgg acggggttcc actcgcggtc gtcgtcgatc gagtccaggt
     6481 gctcgaggaa cgcccggagg gtgagctccc cgtcgacggg ctggaaggcg tgcacctgct
     6541 cgaggaacgc cccgaggttc cgccgccgcg cgagcgccat cggctggtcg gcggccgagt
     6601 cgagctcggc gagcaggccc gtgcgtcgga tgatctcggc caggaactcg ccgaccgggc
     6661 gtcgcgcggc cactcggagc tcgtccagct cgcccccgaa ctcggcgagg cggacccggc
     6721 cctcctcggt gagcccctcg atctcgtcga ggcgttcgag cgcctccgcc atcaggaacg
     6781 ggtggtcctc gagcaggtcg tcgtcctcgt cgagcgcgag gtcggcgagc agctggcgga
     6841 gctcgccccc gctggtccgg gaccacccgg ccagcacgcc caggtcgcgg agcccgacgc
     6901 gataccgagg cccggtcagg atccgcgcga gcgcgactcc gtcacgcggg tcctcggtcg
     6961 cgcgggcgta cgcgagcacc tcgacgacct cgggcaggtt gatcaggccc gcgaggttca
     7021 cgaactcggc gggcacctcc atcgcgccga acgcgagctg cagcgcctcg aacagccggt
     7081 gcgtccggca caggacggcc atctcgcgcc acgcgacgcc gtccgcgtgc agctcgcggg
     7141 cgcgttcggc gacgccctcg gcctccgcga cctcgtgctc gtagcgcgcg acctcgacgt
     7201 ggccctcgcc gttgcgctcc caggggcgca ggagcttgtc ggggtcgggg cgctggttcg
     7261 tgggcagcgc gtcgatcacg cggtctgctg cctggaggat acgggccccg gagcggaagt
     7321 tcgtgtacag cggcagctgc gcggactcgg ccccgtcggc gagcgggaac tgcttgcgga
     7381 agtgcagcag gttccacagg ctcgccccgc gccaggcgta gatgttctgg tcggggtcgc
     7441 cgaccgccgt caccggatgg ccgtcgccga acaccgcccg catcaggacc gcctgggcgt
     7501 ggttcgtgtc ctggtactcg tcgagcagca cggcgccgaa ccggtcgcgg taggccctgc
     7561 cgacgtccgg atgcgccgac gcgatccgga cggcgtgggc gatctgatcc ccgaagtcga
     7621 tcacgccgag ctccgccttg agctcgcgga acacccggac cgcccgggcg acctcgatcc
     7681 gttcctcggc ggccagcagc ggctcggcgg atctcgcctt gcggagcgcc tcgagtttgc
     7741 cctcgacgtg ctcgatcacc gtctcgggtt cgaccaggtg gttctgcagc tgttcgtcga
     7801 gcccgaggat gtagccgacg atcgcgggct gccaccgcgt gtggagcttc tcgaacgtca
     7861 tccggtcgag cacgcgcgcc gcgatctggg ctcgctgcgc ctggctcagc actcgctggc
     7921 cgggctcgag cccgatcaac atcccgtggc gttcgagcac ctgggccgcg aacgcgtggt
     7981 agttcgagat ctcgggctcc tcgccctcgt cgaggtcgag ggtccggagc gcccgacgca
     8041 tcttcaggat caggttctcc gtggccttgt tcgtgaacgt caggcagagc gcgttgcccg
     8101 gcatcacccc ctcgtggtcg gccggccatg ctcccgtcgc gaccatggcg aggtagatca
     8161 cgcgcgcggc catcaccgac gtcttccccg aacccgcgcc ggccaccacg acgcagggtt
     8221 cgagcggcca cgagatcgcc gtccactgct cgggcgtcgg ctcgccgcgg aggaagtcga
     8281 cgatcggcct cgggtagcgc tcggtcatgg gccggccacg acctcggccg gcgccgtcac
     8341 gagcccctcg atcgggaaca cggcatcgcc ctgggggtag cgcgtgcaga gcgtctggaa
     8401 gcggcagaag aagcagctcg ccttcgtgtt cgcgatgtag ctgcgttcct cgttgagctc
     8461 gcggatccgg tcgatcagct cgccgagtcg ctcgcgcatc cgcgccttgt actcctcctc
     8521 gatgtcctcg gagaccgacc actccctgac gtcgagcgcc gggtcggcct tcctgccgcc
     8581 gaggaacgcg agctccaccg cctgcaccgg ccggtgctcc gccaggtcgt cgtcctcgtg
     8641 caccgcgagg tagtagatgc cgagctgcag gctctcggcg ggcctgggcg cactgtccga
     8701 acgcccggtc ttgtagtcgg tgatccgcgt gcccccctcg ggcaccggtc cgatccggtc
     8761 gatcttgccc cggatcctcg cgccctcgaa ctcgaaggcg aacccgcgct cggtggcggt
     8821 cgcgggcggc tcggcgtagc gttcgaacca gttgcggatc agcaccgtcc tcgcgttggc
     8881 gcgctcggcc tcggagacgg cgtaggacgg gaagcgggtc ttctcccacc gttcgtccag
     8941 ggcgcgttcg aacgcctcgg gcgtgcggcc gatcgacccg cgctcgcact cctcgatgat
     9001 cctgtggacg gtcttgccga cccatgcctg gtagccgccg cccggatcca gcccgagctc
     9061 gctcgagagc acgaactgca gctcgcagtt ctcgagcgtc gacaaccgcg agtacgacag
     9121 ggagagcgcg tcctccgcca tcgcggacag gtcggtccag tcgcgctgga accaccagcg
     9181 cccggggtcg tcgccgagcg cgagcagggc gtcgaggcag gcgagacgct cggccggcgc
     9241 cgcggtccga tcgccgagcc cgcgtcgcca ggccgaggcc gcttcgaacg tcgacacggg
     9301 ctccgcgaac ggggcgctcg ggacgtcgcg ccagggcagg ccgagctcct cgacgaagcg
     9361 cgaggtgagc gtcaccgacg actcgtcgcc gtgcgggtcg gtcgccgtca gcaccaccct
     9421 gcgacgcgcg cgcgagcgca ccatcccgaa caggcgacgc tcgtcggcca ggcgccgccg
     9481 gttgacctcg gagcgggcga tcgcctcatc gagcgtcgcg agatcgaaca tcggctcggg
     9541 ccgggacagg ctcgggaaat cgccctcgag cgcatcgagc acgacgaccg tgtcgaactc
     9601 gcgaccggcc gccgcgtgcg ccgtcagcac gtgcacctcg tcgtggctgc cctcgtcccc
     9661 ggccgcgagg atcggcgccc cctccgccgc gccgagcgac agcaggaagg cctcggtcga
     9721 cggatcggcg gcggtccctg ccgacgcgac ggcgttggaa agctccacca ccgcctcgag
     9781 gtcgacgaac gcatggtgat cggtctcggc ccgctcgacc aggtcggccg cgaaggggag
     9841 ctcgagccag agcacgcgga acgcgtcgag caccgaggcg gcgacggcct cggcccgtgc
     9901 cagcacgccg ccgagcgccg tcaacgcctc cgcgtcctcg ggttcggcca gatcgtgcat
     9961 cgcgagcgcg tcccgcggcg ggcgaccgtg cgcgcgggcg agccggagca gggcccggac
    10021 cgacgccggg gacaaccggc cgagctccga ggtgaggacc ggttcgatca gcgagtctcg
    10081 ctcctgaggg ccagcgacga gccacttgag ggcgagcacg aacgggcgcg tcgccgggac
    10141 caccgccggc gagccggcgt cggcgatcct gcgggggacg tgcgcgtcgt cgagggcgcg
    10201 caggagcccc tcgacgcggg gcccgtggcg ccggacgacc accgcgagct cgcgccacgg
    10261 gacctggtcg tcgacgtgca gacgccggag ctcgcgcgcg accgcggcgt gctgctccga
    10321 gacgtgcccg gcgcgccagg cctcgaccgg gtcgggctcc gggcgatgcc gcgtctcgag
    10381 caggaccgtc gcggcgcccg gattgcgctc ggcgaagcgg cggaggggct cgtcggtcgt
    10441 gccctggaac gagaacaggt gcgcctcgag atcgccgacc gcgacgaagg tccgcgggcg
    10501 gatctccgcg aggaggcgtt cggccgcgaa ggtcgtgtcc tggtagtcgt cgaccatcac
    10561 gtggtcgaac ggcgggtcgc ccgcccccgc cgcgaccgcg gcctgctcga cgaggccggc
    10621 gaagtcgacc tcgctcgacg cgtcgagcac cgagaggtag tggcggagga acgccgcgag
    10681 ctcgtcccac cccgcgagcc cacgttcggc cgcccgccgt tcgatgtcgt ccggtgaacg
    10741 caaagcctcc tgcgcccgga tcaccagctg gcggatctcg tcggcgaacc cgcggaggcc
    10801 caggagcggg ccgaagaccg gccagcgccc ggcgtcctcg ccgtcgagga gctcgcgaac
    10861 ccgggcgaac tgctccgatg ccgacaggac ggtgggcggc gcgtcgtacc cgagccgctc
    10921 gaaccgctcg ccgacgacgt ggaacgcgag gccgtggatc gtgacgacgg tgaggctcgg
    10981 cagggcgacc gggaggcgtt cgagcagcag ccggcgggcc tcgtccctcg cgcgccgcga
    11041 acccacgacg agcgccacgc gctcgggatc ggcgccgcgt tctacgagcc gcgcgaaccg
    11101 ttcgcgaagg accgccgtct tgccggtgcc cgaaccgccc gagacgagca tcgcgccgtc
    11161 ggcatggtcg aggacctcga gctgcctggg atcgggttcg aagggggcca tcgaatcgcg
    11221 atgctaccgc cgtgctcgga cacggccgcc ggccggtgtg atcagccggc gcgcgcggcc
    11281 caccggtaga ggccggagcc gctgatggcg ccgacgagcc gtccgtcgcg caggacgagc
    11341 gccgcgcgat cggtccccag ccgcgccgag acctcgtcga ggcgctcctc gggccgggcc
    11401 accaggacgt gttcgagtgg gatgagcgcg tccctggccg gtcgcagcgg atctcggatc
    11461 ccgagctcgc gcgccgagtt gaacgagatc atcccgatta ccgtcccgtc ctcgatcacg
    11521 gggaaggcct cctcctcgtg gccgcgaagg aaccggtcca acgtctgcga gagcgaaagg
    11581 tccgccggga ccgccggcgg cgggggatcc atcgcctccg cgacggtcgc cgacgccagg
    11641 cgcccgtcga tgccgatccg ctgttccgag gcctgggcgc cgcggaagat gaacatcccg
    11701 atgatcccgc cccagacggc ctcgaacagt cgttcctgcg tgagggcgaa gatcgcgagc
    11761 gcgatcacga tgccgccgac cagcagaccc gaccgagccg cgacgcgcgt cgcccgctgc
    11821 tggtcgcgcg tcaggcgcca caccagcgac tgcagcatgc gaccgccgtc gagcggcagg
    11881 ccgggtagcg cattgaagat cgccatgaac aggttgacgg tcgccacgta gctgaacaga
    11941 cccggcagcg ggccggcgat gtccgccgtc aggcgcgaca ggagccagaa cgtccccgag
    12001 agggcgaggc tcgtccccgg gcccagcgcg gcgatcgcga acgcgggccc gggccccttc
    12061 gcgtccgccc gggcggccgt gaagcccccg aagaacacga gcgtgatgcc gtggacctcg
    12121 atcccgttgg cccgggcggc gatcgcgtgg gcggcctcgt gcaggaacac cgatccgaag
    12181 aacacgagcg ccccgaacag cgcgtaggcg aacgcctcgc cgctcgctat ttcggggaac
    12241 tcctggtcga gccgggccca gaacgtggcg acgagcagaa ccgcgatcca gatccaggac
    12301 gagtcgacgt tcaccggcac gccccggatc gtcgcgatgc ggaaaccgcg gccgaacatc
    12361 ctccgagggt accgccgacc cgttcgagcg gccttcggcg tgggtctgcg agagtgggga
    12421 cgtggatgac gggggccacg aaccgagcga acgcggaccg aggcatcgcc tgcgagcggc
    12481 cgcaacggtg ctccttgcct gcgtggtcgg actggtcggc gcgtggctag ggctcgcgct
    12541 gctcgcgcgc gagaccgtgg cgatcgggcc gttccgcgtg aagctcgacg ttggattcgg
    12601 gcgtggcgag acggtcctcg ggctgccacc gttcggcgcg ctcaccgcgg acacgcacct
    12661 ctcgccgctg cacgtgtcgg cgacgctgca ggacgtcggc gtccagcggc tcacggacgt
    12721 cgtgaacgac gagggcctgg atggcctggt gagcgacgtg gagcgcgacg cccgcgggag
    12781 cgttcggacg atcgcatggc ggacgctcct ggccggcacg atcggcggca tcgcgctcgc
    12841 ggcgctcgtc taccggaacc attggcaccg ggtcgccacc ggcggggtcg ccgcgttgct
    12901 cggggtggcg ttctgcgagg tgctcctgct caccaccttc cagcccgcgg cgttcgccga
    12961 accctcgtac tcggggtcgc tcgcgctcgc gccgcagctg atcggcccgg tgagcgaggc
    13021 gaccggtcgt atcgaggatc tgcgggcggg cctcgagcag atcgtcgacg ggaccgtccg
    13081 cgcgtacacg tccctgcagg cgtcgccccc cgccgacgac gcgatccgcg tgctgcacat
    13141 gtcggacatc cacgcgtcgc cgctcgggat ggacttcgcg caggaggtag cgaggggctt
    13201 cggcgtcgac ttcgtgatcg acacgggcga catcacgtcg ttcggcaccc cggtggaaaa
    13261 cctcatcgcg agcgagatcc cgggcttcgg tgtgccctac gtgttcgtcc gggggagcca
    13321 cgactcgatc gcgctccagg aggcgatcgc gcgagagccg aacgcgatcg tcctcgacgg
    13381 ccgcgagaag acgatcgacg ggctgaccgt gtacgggctc ggacaccccg cgttcactcc
    13441 ggcacgggga gcgcccgtcg acgccgagga cttcgcggcc ctcgcgcgtt cggccgggcc
    13501 ggtgatcgcg gcggatctcg aggccgcacc cgagccggtt gacgtcgtcg ccgtccacga
    13561 cgaccggatg gccgaagcgg tcgccggccg ggtgccgctc gtgatctcgg gacacttcca
    13621 cgagaccacg tcgacggtgc gcaacggcac gctgtacctg cgcgtcggga ccaccggcgg
    13681 ttcgggcgcc ggcatcttcc gggacctcgc ggacatcccg ttctcggccg aggtcctgta
    13741 tttctcgcgg gagtcggatc cccggctgat cgcgtacgac gtgatcgagc agctccccga
    13801 gtcgggcagc ctgacggttc agaggatcac ggtctcggtg gagttcgggg acctggtgct
    13861 cacgccgac
//
LOCUS       DATBRK010000254         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2641612, whole genome shotgun sequence.
ACCESSION   DATBRK010000254 DATBRK010000000
VERSION     DATBRK010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641612"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..628
                     /locus_tag="VIH70_07735"
     CDS             248..628
                     /locus_tag="VIH70_07735"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEY8116289.1"
                     /translation="MLTAENHQREGRGAKAEFLRPVLGVIEHVGENRHIWKSLAGKGG
                     ADLVLRILRDNVVALLRDHFVTAFPGSGNDARRLELAVQHVAGALMGVMTWWLEDDVP
                     VPAEEIYEDFRMLALKGVRPFMNG"
     gene            complement(672..983)
                     /locus_tag="VIH70_07740"
     CDS             complement(672..983)
                     /locus_tag="VIH70_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116290.1"
                     /translation="MRVVTREGVVEFIRSHGGRLYVCADLMRCTGPKCTFFSSSLEPP
                     AEHHAFRRFAGGGFDLYFSDEGLEAPTELRLELGGWRKRRISVYEGFSWVMADEPPEE
                     P"
     gene            1544..1948
                     /locus_tag="VIH70_07745"
     CDS             1544..1948
                     /locus_tag="VIH70_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116291.1"
                     /translation="MSKLRIVALSLGVMAVVVGTSQVAFALGGPNARPETWVDGELFA
                     GVVTPATFDPALGNFDELYMCTDGTTFKNGVPLISDAGPGDTDFNGGRWHLNVSTNCD
                     STADSDADLDLSAFVSTDTYFECPLLPRPGNG"
     gene            2123..2245
                     /locus_tag="VIH70_07750"
     CDS             2123..2245
                     /locus_tag="VIH70_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116292.1"
                     /translation="MRYPEIQVVLADSRRFAEEWTYRFLAAALSYARSGDLVVE"
     gene            2630..>3036
                     /locus_tag="VIH70_07755"
     CDS             2630..>3036
                     /locus_tag="VIH70_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY8116293.1"
                     /translation="MRLPVTTSVRPTSRRLPPAAQPQLHRPLSRRTFLRGSAGAAGLA
                     LGSGLLRQLPASAAPPGTGEPSPIPGGIQPVPGGPVFHIFLPGEGSEPSTITDFNGSV
                     GVSVTQGTWSATGTPTGGVSSGVWEADMRFMKGL"
BASE COUNT          505 a         1006 c         1002 g          523 t
ORIGIN      
        1 aacggaagcg cagagatcgt tcgggaaagg acgcgagatg gcggagcggg tgaagatgga
       61 tcgacgggtc cgacgaacac gtgagctcct ccgaacagcg ttaaccacgc tgatccaaga
      121 gcgcggatac gaccggatca cagtccagga catcctcgac gaggccgacg tcgggcgatc
      181 gaccttctac gcgcacttcc gcgacaaaga cgacctgttg cgcagcggat tccaagacct
      241 acgttcgatg ctgaccgccg agaaccatca gcgagaaggg cgaggcgcga aggctgagtt
      301 ccttcgcccg gtgttggggg taatcgagca tgtcggggag aaccgccaca tctggaagtc
      361 gctcgcgggt aagggcggcg cagacctggt ccttcgcatc cttcgcgaca acgtggtggc
      421 gctcctccgc gatcacttcg taacggcctt ccccggctct ggaaacgacg cccggcggct
      481 cgagcttgcg gtccaacacg ttgcgggcgc tctcatggga gtgatgacct ggtggctcga
      541 ggatgatgtc ccggtcccgg ccgaggagat ctacgaggat ttccggatgc tcgcactgaa
      601 gggtgtgagg ccattcatga acggctgatc gacccacgct gatcttttgc cacgcgcatc
      661 ggcggtcgcc attagggttc ctccggaggc tcgtctgcca tgacccagga gaagccctcg
      721 tacaccgaga tgcgcctctt ccgccagcct cccaactcga ggcgtagctc ggtgggcgcc
      781 tccagcccct cgtcgctgaa gtagaggtcg aagccgccgc cggcgaaccg ccggaacgcg
      841 tggtgctccg cgggcggttc gagcgaggac gagaagaacg tgcacttcgg gccggtgcac
      901 cgcatgagat cggcacacac gtagagcctg ccgccatggg agcggatgaa ctccaccaca
      961 ccctcgcggg taaccaccct cacgtctcag cctcctcgtt gcggtgatcg cgccgccgga
     1021 gtctcatcga gcccttgtcg caggagtcag tggaggtcga acgcgggccg tcggcatcgg
     1081 ttcaaaatcg catccacacg cgaccacagc gaagatcgtg tcccagcaag gacgggatcg
     1141 tgttcctcgc atcggcggcg cgaggtacgg gccgtggcac tgtcgcgggt ccgccagatg
     1201 atgtcgacga accgcgcggc ggacggcttc ctcctcgtac tcgaaggtca cggatgttcc
     1261 taaggaaccg ccatggtaaa cccagttcgt caccctcgat gcctccgcgc gccatcggac
     1321 gccggtgtca ggttgtctcg gaacgttcac cgatgtgtcc gaaaccttcc ctcagctgtg
     1381 tctcgagtca gcacggccgc ggaaattccg ggcggcgtaa ggcccacgta agcccttcgt
     1441 aagacgcgtc tcctagcgtc atcagtggtg tgccgggctc cggccgggag cgcggcgatt
     1501 acatgccctg gcttgtcgac ggcaagcaga gaggagggac ggcatgtcga agcttcggat
     1561 cgtcgctctt tcgctcggag tcatggcggt cgtggtgggc acctctcagg tcgcgttcgc
     1621 tctggggggc ccgaacgcgc ggccggagac gtgggtggac ggcgagctct tcgcaggcgt
     1681 ggtgacgccg gcaacgttcg accccgcgct gggcaacttc gacgagttgt acatgtgcac
     1741 cgacggcacg acgttcaaga acggcgtccc gctgatctcg gatgccggcc cgggagacac
     1801 ggacttcaac ggcggccgtt ggcacctgaa cgtgagcacc aactgcgact cgaccgccga
     1861 ttcggacgcg gatctcgatc tctcggcctt cgtgtccacc gacacgtact tcgagtgccc
     1921 gctgctgcca cggcccggga acggctagct cgtccttcac cggctcgtac ccccttcgcc
     1981 gcgcgtgagg cacgaggccg tcagccctgg gcgaccgggt agatctcgtc cgcgaccagc
     2041 ccgtggacct aggacgacgc cgaatccgtg ctgaccgggc ccagcgtcgg ctcgcgaacc
     2101 ggctcctcgg cgggggctcc aggtgcgtta ccccgagatc caggtcgtct tggccgattc
     2161 gcggcggttc gcggaggagt ggacataccg gttcctcgcg gcggcgcttt cgtacgcgcg
     2221 aagcggagat ctggttgttg agtgaaggtg atcgggctgt cggacccttt caggggactc
     2281 cacgtccttc tcgtcctggt tgcggcccgg ctcaacgcgc tcgttgatgg ccagacgtag
     2341 acttcggtca tgcgagtgga tcccgaacgc ccaccgtact gcccggactg tccgcctgat
     2401 ccttcggcct gagcatccga gcccccttcg cggagtctcc gaccaacggg atcttgggcg
     2461 cgcgacgact tgtccggaaa tcgcgggcat gatgatgcag ctggggttca tccccagatg
     2521 tagttcctcc ggccttcccc acttcgcgga cccggcgtaa gatcgcatga tcagaagggt
     2581 ggcccgccgc acggggaccc gtctggggcc ctcggggaag gagggagcga tgcgcctccc
     2641 agtcacgacc tcggttcgcc cgacctcccg acgtctccca cccgccgccc aacctcagct
     2701 gcatcgccct ctctcccgac ggaccttcct ccgcggttcg gcgggcgcgg ctggattggc
     2761 gctgggctcg ggcctgctgc ggcagctgcc ggcgtcggcc gccccgcccg gcacgggaga
     2821 gccctcgccg atccccggcg ggatccagcc cgtccccggc ggccccgtgt tccatatctt
     2881 cctcccgggg gaaggaagcg agccctctac gatcacggac ttcaacgggt ccgtgggcgt
     2941 atcggtgacg cagggaacat ggagcgcgac cgggacaccg accggagggg tgtcctccgg
     3001 cgtgtgggaa gcggacatgc gcttcatgaa gggcct
//
LOCUS       DATBRK010000255         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1486386, whole genome shotgun sequence.
ACCESSION   DATBRK010000255 DATBRK010000000
VERSION     DATBRK010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486386"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VIH70_07760"
     CDS             complement(<1..483)
                     /locus_tag="VIH70_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8116294.1"
                     /translation="MDIPQERKVVTEIPGPRSREWFERRSNAVPQGVFNTHPIVTARA
                     SGAIIEDVDGNRLIDFATGIAVLNVGHTSPEVVAAAQRQLELDTHTCFHVTANEPYIE
                     LAERLNALAPGDGPKKTMFANSGAEAVENAVKIARKATGRQAIVTFDHAFHGRTLLAM
                     S"
     gene            complement(551..991)
                     /locus_tag="VIH70_07765"
     CDS             complement(551..991)
                     /locus_tag="VIH70_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116295.1"
                     /translation="MPMRRYLVVANQTLAGEPLVSRIRELSRAGPCAFFLVVPATPPS
                     DHPWTEGEARAAAGARLDEARMRLSGLGVEVEGGEVGDGNPMLAIGDAIRDRGPFDEL
                     ILSTLPPGLSKWLKLDLPHRVAASFAIPMTHVVGEPTHVGASRR"
     gene            1136..>2311
                     /locus_tag="VIH70_07770"
     CDS             1136..>2311
                     /locus_tag="VIH70_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8116296.1"
                     /translation="MGDARSYKMYVGGEWVDAAEGQTRKIVSPANGDVIAEVPEASAA
                     DVDRAVAAAKKAYDGTWSEATPGERSRALLRMADLVEEHGDELGRLESENVGKVLALT
                     MSEEIPVIADQFRFFAAGARVLGGLAAGEYMKGYTSILRREPIGVAGLIAPWNYPLYM
                     SAWKLGPALAAGNTVVIKPAENTPLTLLRFTQLLAEAEIFPPGVFNVVTGDGLPAGDA
                     IVKNKDVGIVSLTGESSTGKLIAKNAADTLKRVHLELGGKAPVVVFDDADVAAAAEWI
                     QVAGYFNSGQDCTAATRVIAGPKVFDNLVSEVVSKVSEMKVGDIFDDEAAMGSVVSDV
                     QLDRISGFVDRAREGGATINIGGEVIDQPGSWYAPTVVTDVAQDSEIVQNEVFGPVVT
                     "
BASE COUNT          358 a          788 c          820 g          345 t
ORIGIN      
        1 cgacatcgcg agcagcgtcc ggccgtggaa cgcgtggtcg aacgtcacga tcgcctggcg
       61 ccccgtcgcc tttcgcgcga tcttcaccgc gttctcgacg gcctcggcgc ccgagttcgc
      121 gaacatcgtc ttcttcggcc cgtcgccggg agcgagggcg ttcagccgct cggccagctc
      181 gatgtagggc tcgttcgcgg tcacgtggaa gcatgtgtgc gtgtcgagct cgagctgccg
      241 ttgggcggcc gcgaccacct cgggtgaagt gtggcccacg ttgagcaccg cgatcccggt
      301 ggcgaagtcg atcagccgat tgccgtcgac gtcctcgatg atcgcgcccg aagcccgggc
      361 ggtgacgatc ggatgggtgt tgaacacgcc ctgcgggacc gcattcgagc ggcgctcgaa
      421 ccactcgcgc gacctgggcc ccgggatctc ggtgacgacc ttgcgctcct gcgggatgtc
      481 cacgtgccga cgcctcctcg tgaggatagc gtcagtttcc cacgcgaccg aacggccggg
      541 cgaaccgctg ttaccttcgc gaggctccca cgtgcgtggg ctcgcccacg acgtgcgtca
      601 tggggatcgc gaagctcgcc gcaacgcggt gggggagatc gagcttgagc cacttcgaca
      661 ggccgggcgg caacgtcgac aggatgagct cgtcgaacgg cccgcggtcg cggatcgcgt
      721 cgccgatcgc gagcatcggg ttcccgtcgc cgacctcgcc gccctccacc tccacgccga
      781 gtccggacag gcgcatccga gcctcgtcca gcctcgctcc ggccgcggct cgcgcctcgc
      841 cctcggtcca tgggtggtcg ctcgggggcg tggcgggcac cacgaggaag aaggcgcagg
      901 gcccggcgcg cgagagctcc cggatccggg agacgagtgg ttcgccggcg agcgtctggt
      961 tggcgacgac caggtaacgg cgcatcggca ccacctccca ccgtccaccc tacgaccctg
     1021 gacccgaaac ggggcctgcc ggcctcggcg agaccccgat ccgcgctttt cggcatactt
     1081 tggtgaggac gacctccaga cccgcggggt cccagcgcag gaaggtgagg tccggatggg
     1141 cgacgcgagg agctacaaga tgtacgtcgg cggcgagtgg gtggacgccg ccgagggcca
     1201 gacccgcaag atcgtcagcc ccgccaacgg cgacgtgatc gccgaggtgc cggaggcgtc
     1261 cgcggccgac gtcgaccggg ccgtggccgc cgccaagaag gcctacgacg ggacgtggtc
     1321 cgaggccaca ccgggggagc ggagccgagc actgctccgg atggccgacc tcgtcgagga
     1381 gcacggggac gagctcggac gcctcgaatc ggagaacgtg ggcaaggtcc tcgccctcac
     1441 gatgtccgag gagatcccgg tcatcgccga ccagttccgg ttcttcgccg cgggtgctcg
     1501 cgtcctcggc gggctcgccg ccggcgagta catgaagggg tacacgagca tccttcggcg
     1561 cgagccgatc ggcgtcgcgg gcctgatcgc tccctggaac taccccctct acatgtccgc
     1621 gtggaagctc gggccggcgc tcgccgccgg caacacggtg gtgatcaagc cggcggagaa
     1681 cacgccgctc acgttgctcc ggttcacgca gctcctcgcc gaggccgaga tcttcccgcc
     1741 cggcgtcttc aacgtcgtga cgggagacgg cctgcccgcc ggcgacgcga tcgtgaagaa
     1801 caaggacgtc gggatcgtct ccctcacggg agagagctcg accggcaagc tcatcgccaa
     1861 gaacgcggcc gacacgctca agcgcgtgca cctcgagctc ggcggtaagg cgccggttgt
     1921 cgtgtttgac gacgcggacg tcgcggcggc ggccgagtgg atccaggtcg cgggctactt
     1981 caactcgggc caggactgca cggccgcgac ccgcgtgatc gcgggaccca aggtgttcga
     2041 caacctggtt tccgaggtcg tctcgaaggt ctcggagatg aaggtaggcg acatcttcga
     2101 cgacgaggcc gcgatgggtt cggtggtctc cgacgttcag ctcgaccgga tcagcggctt
     2161 cgtcgatcgt gcgcgcgagg gcggcgccac gatcaacatc ggcggcgagg tgatcgacca
     2221 gccgggctcg tggtacgcgc cgacggtcgt gaccgacgtc gcccaggact ccgagatcgt
     2281 gcagaacgag gtgttcggcc cggtggtgac g
//
LOCUS       DATBRK010000256         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2784020, whole genome shotgun sequence.
ACCESSION   DATBRK010000256 DATBRK010000000
VERSION     DATBRK010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2784020"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1452
                     /locus_tag="VIH70_07775"
     CDS             301..1452
                     /locus_tag="VIH70_07775"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY8116297.1"
                     /translation="MVTKDQVREALRTVNDPEIGRPIEDIGMLRDIEVLEGGLVRVHV
                     LITIEGCPLKDRITSEVTEALAPVEGVERVEVSLSPMSEEQRASLVSTLRGGTAPQQQ
                     PTFFAGSDTTVIAVASGKGGVGKSSVTVNLACALAAEGHRVGILDADVWGFSVPRMMG
                     VTGKPVGFNDMILPMESHGVKVISMGFFVPEETPVIWRGPMLHKAIEQFLGDVYWGDL
                     DFLLCDLPPGTGDVSISLASFLPGASMLVVTTPQEAARKVAERAGKMAERTNLRPIGV
                     IENMSWFVCPHCGEPTPIFGQGGGDQAAETLGVPLFAQVPLEPALREGGDDGAPIVVT
                     DPDSPAGEALRRAARELAHTTRSKVGRPLPLMARPGAPVTAGDHHGHAH"
     gene            complement(1454..>2204)
                     /locus_tag="VIH70_07780"
     CDS             complement(1454..>2204)
                     /locus_tag="VIH70_07780"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY8116298.1"
                     /translation="IIGARLFFVIGHFSEFDGVADMLALWNGGLTLLGGIAGAVLINI
                     PLFRRYHYSFFQVMDGAVIGLAFGIAFGRIGDLIIGDHLGKPTSWLLAFRYEGGNLSG
                     FACAEGSCRATLEGGQVLTITHQVARLTSPTGRLLAEGVGVHQTALYDMFFATCLFLV
                     LYALSRAPRRLGVLTLVFGAVYGATRVVEDFLRVDKRVFGLTGSQWTGLTVSIVCSIT
                     LIAWAVGARSSPGEEERAPSGDADPADRGVP"
BASE COUNT          365 a          768 c          780 g          291 t
ORIGIN      
        1 gagagctcgc ggaccgagac cggacggccc gacagcccga ggtagcgata cagccggaac
       61 cgcgtgtcgt cggcgagcgc cttgtggatc tcgagcgggg tcggctcacg ggcgggcacg
      121 ggcggctcct ggcgatcggc gagacgggcg cgcgtcgacg cacgcgagcg ccggacgtac
      181 catgggagaa agcacggttc gcaccgtgcc aatcaggata atccgagccc caccgatccg
      241 aaccgggatc cgagacagag cccccgcccg cgagcgtggc gggtgcgagg aggtcatcgc
      301 ttggtcacca aggaccaggt tcgagaggcg ctgcgaacgg tcaacgaccc ggagatcggc
      361 cggccgatcg aggacatcgg catgctccgg gacatcgagg tcctggaggg cggtctggtt
      421 cgcgtgcacg tcctgatcac gatcgagggc tgcccgctca aggaccggat caccagcgag
      481 gtcaccgagg cgctcgcgcc cgtcgagggc gtcgaacgcg tcgaggtctc gctctccccg
      541 atgagcgagg agcagcgcgc ttcgctggtc tcgaccctgc gcggcggcac ggccccccag
      601 cagcagccga ccttcttcgc gggctccgat accacggtga tcgccgtcgc ctcgggcaag
      661 ggcggcgtcg gcaagtccag cgtcacggtg aacctcgcgt gcgccctcgc ggccgagggc
      721 caccgcgtcg ggatcctcga cgccgacgtc tgggggttca gcgtgccccg catgatgggt
      781 gtgaccggca agcccgtcgg gttcaacgac atgatcctgc cgatggagtc ccacggcgtg
      841 aaggtgatct cgatgggctt cttcgtgccc gaggagacgc cggtgatctg gcgcggcccg
      901 atgctgcaca aggcgatcga gcagttcctg ggcgacgtgt actggggcga tctcgacttc
      961 ctcctgtgcg acctgccgcc ggggaccggc gacgtgtcga tcagcctggc gtcgttcctg
     1021 cccggcgcgt cgatgctcgt ggtcaccacg ccgcaggagg cggcgcgcaa ggtggcggag
     1081 cgggcgggca agatggcgga gcggaccaac ctgcgtccga tcggcgtcat cgagaacatg
     1141 tcgtggttcg tctgcccgca ctgcggcgaa cccacgccga tcttcggtca gggcggcggg
     1201 gaccaggccg cggagaccct cggcgtcccg ctgttcgcgc aggtgccgct cgagcccgcg
     1261 ctgcgcgagg gcggcgacga cggcgccccg atcgtcgtga ccgacccgga ttcacccgcg
     1321 ggcgaggccc tccggcgcgc cgcgcgggag ctcgcgcaca cgacccgatc gaaggtcggc
     1381 aggccgctcc cgctgatggc tcgacccggc gctcccgtga cggccggcga ccatcacggg
     1441 catgcgcact agatcaggga acgccgcgat cggccggatc cgcgtcgccc gagggcgcgc
     1501 gctcctcctc ccccggtgac gaacgcgcac ctaccgccca cgcgatcaag gtgatgctgc
     1561 agacgatcga gaccgtgagc ccggtccact ggctgccggt caggccgaac acccgcttgt
     1621 cgacgcgcag gaagtcctcc acgactcgcg tcgccccgta gaccgcgccg aagacgaggg
     1681 tgagcacccc gaggcgccgg ggcgccctcg acagcgcgta cagcaccagg aacaggcacg
     1741 tcgcgaagaa catgtcgtac aacgcggtct gatggacccc gacgccctcc gcgaggagcc
     1801 ggcccgtcgg tgacgtgagc ctggccacct ggtgggtgat cgtgagcacc tggccaccct
     1861 cgagcgtcgc gcggcagctg ccctcggcgc aggcgaagcc cgacaggttg ccgccctcgt
     1921 accggaacgc gagcagccag ctggtcggct tgccgaggtg gtcgccgatg atcaggtcgc
     1981 cgatcctgcc gaacgcgatc ccgaacgcga gcccgatcac cgcgccgtcc atcacctgga
     2041 agaacgaata gtgatacctg cgaaacaggg ggatgttgat caggaccgcg ccggcgatcc
     2101 ccccgagcag cgtgagccca ccgttccaga gcgcgagcat gtcggccacg ccgtcgaact
     2161 cggagaagtg accgatcacg aagaacagac gggcgccgat gatc
//
LOCUS       DATBRK010000257         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2784820, whole genome shotgun sequence.
ACCESSION   DATBRK010000257 DATBRK010000000
VERSION     DATBRK010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2784820"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VIH70_07785"
     CDS             <1..205
                     /locus_tag="VIH70_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8116299.1"
                     /translation="VETIDGKAGGLAVVIGARIGGLAGASEVLSSQTVRDLTAGSGIV
                     FEEAGEHELKGVPGRWRVYRAAD"
     gene            345..1871
                     /locus_tag="VIH70_07790"
     CDS             345..1871
                     /locus_tag="VIH70_07790"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY8116300.1"
                     /translation="MRLEEIVATSAAIAQTRSRKTKTELLASSLRRLEPREVPIAVAY
                     LSGRLPQGTIGVGWASLRERPPAAAGSTLGLIEVDAALERIRLAVGRGSQEARRRELS
                     ALFGAATEPEQEFLSRLLIGELRQGALEGSVVEGVAAAAGVPAAQVRRALMLEGDLGT
                     VAAAAIAEGEAGLRRFSLAVLRPVYPMLAQTAEDTADALDRIHPADVEWKLDGVRLQV
                     HVLKGQVRAFARTLADVTERVPEVVDAMSGVPFEAAILDGEAIALRPDGRPHPFQVTG
                     SRFASRVDVEGQRRSTPLSPFFFDLLHLDGDDLIDRPARERLGIMDRRLPSGLLVPRT
                     VAGDAETADGFLDDALAHGHEGVMVKALDAPYEAGRRGSGWLKVKRAHTLDLVVLAAE
                     WGHGRRQGWLSNLHLGARDPNGGFVMLGKTFKGMTDEMLRWQTERLAALEIGREGHVV
                     HVRPELVVEVAFDGVQASPHYPGGLALRFARVRRYRPDKRAEDADTIDAVRAIAARGR
                     "
     gene            complement(1880..>2168)
                     /locus_tag="VIH70_07795"
     CDS             complement(1880..>2168)
                     /locus_tag="VIH70_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116301.1"
                     /translation="DHPEVKWAVGLDWNATSHVTPAVAFLGTHQPNTPSFDDSYAVSV
                     GARVSLWGDTLVGFANVLFPIGDQGSGLGKVRLGPFSEVNAIPLVGIEATL"
BASE COUNT          304 a          739 c          817 g          308 t
ORIGIN      
        1 ggtcgagacg atcgatggca aagccggcgg gcttgcggtc gtgatcgggg cgaggatcgg
       61 cggactcgcg ggggcgtcgg aggtgctcag ctcgcagacg gtgcgagatc tcacggccgg
      121 ctcggggatc gtgttcgagg aggccggcga gcacgagctc aagggcgtcc cggggcggtg
      181 gcgcgtatac cgggccgccg actgatgccc ttgcggcgga cgtcacccaa atgggattcc
      241 tgcggtcggt cacttgtcac gccgaacgcg gtggaagcac tttggccgcg aacggcatcc
      301 gtgggtcaag gatccgggca ccgagcgttc tacgggccgc ttcgatgagg ctggaagaga
      361 tcgtcgcgac gtccgcggcg atcgcacaga cgcgctcgcg caagacgaag accgagctgc
      421 tcgcctcgtc cctgcgacgc ctggagccga gagaggtgcc gatcgcggtt gcctacctgt
      481 ccggcaggtt gccgcagggg acgatcggcg tcgggtgggc ctcgctccgc gaacggccgc
      541 cggccgcggc cggatcgacg ctcgggttga tcgaggtcga cgccgcgctc gagcggatcc
      601 gcctcgcggt cggcaggggc agccaggagg ctcgcaggag agagctctcg gcgctgttcg
      661 gcgccgcgac cgaacccgaa caggagttcc tgtcccgcct gctgatcggc gagctgcggc
      721 agggtgccct tgaggggagc gtggtcgagg gcgtggccgc agcggcgggg gtgccggccg
      781 cgcaggtgcg ccgggcgctc atgctcgagg gcgacctggg taccgtggcg gccgcggcca
      841 tcgccgaggg cgaggccggc ctgcgccggt tcagcctcgc cgttcttcgg cccgtgtacc
      901 cgatgctcgc gcagacggcg gaggacacgg ccgacgccct cgatcggatc catcccgccg
      961 acgtcgagtg gaagctcgac ggtgtccggc tgcaggtcca cgtcctgaaa gggcaggtgc
     1021 gggcgttcgc ccgcacgctg gccgacgtca ccgagcgggt gcccgaggtc gtcgacgcga
     1081 tgtccggcgt gccgttcgag gcggcgatcc tggacggcga ggcgatcgcg cttcggcccg
     1141 acggccggcc gcacccgttc caggtcaccg ggagccgttt cgcgagcagg gtcgatgtcg
     1201 agggccagcg gcggtcgacg ccgctctccc cgttcttctt cgacctcctg catctggacg
     1261 gcgacgacct catcgaccgc ccggcacgcg agcgcctggg gatcatggac cggcgcctcc
     1321 ccagtgggct cctggtgccg cggacggtcg ccggggacgc cgagaccgcc gacgggttct
     1381 tggacgacgc cctcgcgcac ggccacgagg gcgtgatggt caaggcgctc gacgcgccct
     1441 acgaggccgg ccgccgcggc tccggctggc tcaaggtcaa gcgcgcacat acgctcgatc
     1501 tggtcgtcct ggccgccgag tggggccacg ggcgacgcca gggttggctc agcaatctcc
     1561 acctcggcgc ccgcgatccg aacggcgggt tcgtcatgct cggcaagacc ttcaagggca
     1621 tgacggacga gatgctgcgg tggcagaccg agcggctcgc cgcactcgag atcggccgcg
     1681 aggggcacgt cgtgcacgtg cgccccgagc tcgtcgtcga ggtcgcgttc gacggcgtgc
     1741 aggcgagccc gcactatccg ggcggcctcg cactccgctt cgcgcgcgtg cggcgctacc
     1801 gccccgacaa gcgcgccgag gacgcggaca cgatcgacgc ggtacgcgcg atcgcggcgc
     1861 gggggcgatg aggcacgcgc tacaacgtcg cttcgatgcc gaccagcggt atggcgttca
     1921 cctcgctgaa gggtcccaag cggaccttcc ccaggccgct tccctggtcg ccgatcggga
     1981 acagcacgtt ggcgaagccg acgagcgtgt cgccccagag gctcacgcgc gcgccgaccg
     2041 acacggcata gctgtcgtcg aacgacggcg tgttcggctg gtgcgtgccg agaaacgcga
     2101 ccgccggcgt gacgtggctc gtcgcgttcc agtcgaggcc gaccgcccat ttcacctcgg
     2161 gatgatcg
//
LOCUS       DATBRK010000258         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_187810, whole genome shotgun sequence.
ACCESSION   DATBRK010000258 DATBRK010000000
VERSION     DATBRK010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187810"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VIH70_07800"
     CDS             complement(<1..968)
                     /locus_tag="VIH70_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY8116302.1"
                     /translation="MSHDQRIPYGAWPSPITADRAAAASVGLSETRAIGDATTWLERR
                     PQEEGRSVVVRSAPGAEPVDVTPPGVSVRTRVHEYGGGSYAVAGATTYFVNLEDQRLY
                     RQEVDGAPAAITPEPPIPQGLRHADMNVSPDGRWIASVHERHRDEGMPVNELVLIAAD
                     GSAMSTLASGRDFYAFPRWSPDGGRLAFIAWDFPRMPWDGTELLVVDVGSDGSAGPSR
                     PVAGGERESIFQPAWAPDGSLTFVSDRTDWWNLYREGADGEITNLTPMSAEFAVPMWL
                     FGYSSYDFLSDGRIVCTYRQRGVHHLALLDPATRELLDLDLPYETF"
     gene            complement(965..1900)
                     /locus_tag="VIH70_07805"
     CDS             complement(965..1900)
                     /locus_tag="VIH70_07805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEY8116303.1"
                     /translation="MKSPRKAKPRNAKPRNAKPTKAKPTKAKEVLTDAVATLEASTAI
                     DHWQRDREEIEADELLAYALGVDRVGPNDEVPPNVRRRFDRMIARRASGEPVQLITGQ
                     AFFRGLQIAARRGVFVPRDSTEYLAEQAVRRLRGRRRPVAVDLATGGGTVALAIANEV
                     PGVRVFGTDISLTAVRVARENAELLRLRATFAKGDLFGGLPSDLRGRVDVITLHPPYV
                     ARDEVRELPEEIRRFEPEHTLTDRSRDGLGLVGRTASEGPRWLKPGGWLLVEVSPDRA
                     RSAASVLRRNGFEDVRSTMDRGFRVTRVLVGRWHG"
     gene            1973..>2217
                     /locus_tag="VIH70_07810"
     CDS             1973..>2217
                     /locus_tag="VIH70_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116304.1"
                     /translation="MPEAARSPKPRTSKPRKQRTTSAKAEDIRLTGDRIVVENPDSGE
                     RTSRGGLLIPATAAPAPKRCVWGEVLLVGPDVRSATAG"
BASE COUNT          323 a          815 c          764 g          315 t
ORIGIN      
        1 tcgaaggtct cgtacggcag gtcgaggtcc aggagctccc gcgtcgccgg atcgagcagc
       61 gcgaggtgat ggacgcctcg ctgtcggtac gtgcacacga tcctcccgtc ggagaggaag
      121 tcgtacgacg agtagccgaa gagccacatc ggtacggcga actcggcgga catcggcgtc
      181 aggttcgtga tttcgccgtc ggctccctcc cggtagaggt tccaccagtc cgtgcggtcc
      241 gagacgaagg tcaacgaacc gtcgggggcc caggccggct ggaagatcga ctcccgctcg
      301 ccccccgcga ccgggcgcga cggcccggcc gacccgtccg acccgacatc gaccaccagg
      361 agctcggtcc cgtcccaggg catccgcggg aagtcccacg cgatgaacgc gaggcgcccg
      421 ccgtcgggcg accaccgcgg gaaggcgtag aagtcccgtc cggacgcgag cgtcgacatc
      481 gccgatccgt cggcggcgat caggacgagc tcgttgaccg gcatgccctc gtcccgatgg
      541 cgttcgtgga ccgacgcgat ccaccggccg tccggcgaca cgttcatatc ggcgtgccgc
      601 agaccctggg ggatcggggg ttcgggggtg atcgcggcgg gcgctccgtc gacctcctga
      661 cggtacagcc gctggtcctc gagattcacg aagtacgtgg tcgcgccggc caccgcgtac
      721 gagccgccgc cgtactcgtg gacccgggtc cggacggaca cgcccggcgg cgtcacgtcc
      781 acgggctccg cgccgggggc cgaccgaacg acgaccgaac gaccctcttc ttgcggccga
      841 cgctcgagcc acgtcgtcgc gtcgccgatc gcgcgtgtct cgctcagccc gaccgaggcc
      901 gcggcggcgc gatccgccgt gatcggcgac ggccacgctc cgtaggggat gcgttgatcg
      961 tggctcatcc gtgccaacgg ccgacgagca cgcgcgtcac cctgaacccg cggtccatcg
     1021 tgctccgcac gtcctcgaaa ccgttccgcc gcaacacgga cgcggcggat ctcgcgcggt
     1081 cgggactcac ctcgaccagc aaccagccac ccggcttcag ccaccgagga ccctcggacg
     1141 cggtccgccc gacgagcccg agtccgtccc ggctgcggtc ggtgagcgtg tgctcgggct
     1201 cgaagcgccg gatctcctcc ggcagctcgc gcacctcgtc gcgagcgacg tacggcgggt
     1261 gcagggtgat cacgtccacc cgaccccgca ggtcactcgg caggccaccg aacaggtcgc
     1321 ccttcgcgaa cgtcgctcgc aggcggagca gctcggcgtt ctccctggcg acccggacgg
     1381 cggtgagcga gatgtcggtg ccgaacaccc ggacccccgg gacctcgttc gcgatggcca
     1441 gggccacggt cccgccaccg gtcgcgaggt cgacggcgac cgggcgccgg cgtccgcgga
     1501 gtctccgcac ggcctgctca gcgaggtact cggtcgagtc ccgcggcacg aacaccccgc
     1561 gccgcgccgc gatctgcagc cctcggaaga aagcctgtcc cgtgatgagc tggacgggct
     1621 cgcccgatgc ccgacgggcg atcatccggt cgaagcgtcg gcggacgttg ggtgggacct
     1681 cgtcgttcgg gccgacgcgg tcgacgccga gcgcgtaggc gagcagctcg tcggcctcga
     1741 tctcctcgcg atcgcgctgc caatgatcga tcgccgtgga cgcctcgagc gtcgcgacgg
     1801 catcggtgag gacctccttc gccttcgttg gcttcgcctt cgttggcttc gcgttccttg
     1861 gcttcgcgtt ccttggcttc gccttccttg gggacttcat cgggcgagca tcgtacccgc
     1921 ggggaggctc ggccacctgg ccgctcccgt cgtcctggct acaatcgctg ccatgcccga
     1981 ggccgcgaga tccccgaagc cccgcacctc gaagccccgc aagcaacgga ccacgtcggc
     2041 aaaggccgag gacatccgtc tcaccggcga ccggatcgtc gtggagaacc cggacagcgg
     2101 cgaacggacg tcaaggggcg ggttgctgat cccggcgacc gccgcgccgg cccccaagcg
     2161 atgcgtgtgg ggcgaggtgc tcctggtcgg accggacgtc cgctcggcga cggcggg
//
LOCUS       DATBRK010000259         7249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2352794, whole genome shotgun sequence.
ACCESSION   DATBRK010000259 DATBRK010000000
VERSION     DATBRK010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2352794"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VIH70_07815"
     CDS             complement(<1..221)
                     /locus_tag="VIH70_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116305.1"
                     /translation="MSDTTPADGVPGASERAARGPVLRKGVGPSPTGWFDPRGRAIGS
                     WAFAVNRITGLGLVFYLYLHLGVLSLLLAG"
     gene            complement(218..970)
                     /locus_tag="VIH70_07820"
     CDS             complement(218..970)
                     /locus_tag="VIH70_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY8116306.1"
                     /translation="METIEGTATAATEASETRRFRVFRFKRGDRGPHLDEFDVPVTAR
                     TTLFDAMRWIQLNRDPSLALRHSCLHASCGTCGVRANGREVLMCVTAVHDLGREITVE
                     PVANIPVLTDLVVDMTEFFARFPAAHPLVRESGFDLGAEAPEGVATYQRYEDCIECGL
                     CLSACPVVATTDRYVGPGALAWAQRILEEPRDEDVLSVREWADDPDGAWRCHAAFECT
                     EACPSNVNPAARIMQLRGELVGPRLTRRAMPR"
     gene            complement(978..2168)
                     /locus_tag="VIH70_07825"
     CDS             complement(978..2168)
                     /locus_tag="VIH70_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8116307.1"
                     /translation="MNAPASRTSRPHVLIVGGGGTGGALAHDLTLRGVKVTLVERGEI
                     TSGTTGRHHGLLHSGARYAVGDRESAIECIEENTILRRICPGSFEENDGLFVAITDED
                     MEYLEPLLRGCADCGIPTQTLTRDEALRLEPLLNPELKAAVRIPDATMDAMRMPLRFF
                     ATAKHGGAGIHAYMEVEGLFVHDHLVSGAAVRDHVTGKQGEIRADIVVNATGPWAERI
                     AAMAGVDVPIRPSPGVLLALRGRLCNMVINRMHRSGDGDIVVPQRALSIVGTSSWTVD
                     DPDDLGVPEDHVRRMYEEGSKLIPAVKTAEFRAAWSAARPLIGSKDEVDSGRELSRTF
                     KTYDHKETDGVEGFVTITGGKGTTLRGMAELCANVICAKLGIEAECQTRDTVLLPHEA
                     YYAA"
     gene            complement(2205..2474)
                     /locus_tag="VIH70_07830"
     CDS             complement(2205..2474)
                     /locus_tag="VIH70_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116308.1"
                     /translation="MEKEHRHGDFAEGQEDSEEHEHHAGSFAEGQAEEHPHPQESKHG
                     DFAEGQEDPEEHEHDEGSFAEGQEGPEGHEHEEGSLPEDQRPSDD"
     gene            complement(2529..4025)
                     /gene="glpK"
                     /locus_tag="VIH70_07835"
     CDS             complement(2529..4025)
                     /gene="glpK"
                     /locus_tag="VIH70_07835"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY8116309.1"
                     /translation="MADFAAALDQGTTSTRFMIFDHEGQVKGIEQKEHEQIYPKPGHV
                     EHDALEIWQRSEEVIAGALNKAGLKASDLSAVGITNQRETTVVWEKTSGKPVYNAIVW
                     QDTRTDQICNQLSSDGGQDRFRPKTGLPIATYFSGPKIKWILDNVDGVRERAEGGEIL
                     FGNIDTWCIWNLTGGPDGGAHVTDVTNASRTLMMDLATLQWDDEILGILGVPRAMLPE
                     IKASSEVYGEARGDLAGIPVAGDLGDQQAALFGQTCYSVGEAKNTYGTGNFLLLNTGT
                     EPVPSKSGLITGLGYKIGDQPATYMLEGSIAITGALVQWLRDNLGMIEKSEDIEALAA
                     TVEDNGGTYFVPAFSGLFAPYWKNDARGVIAGLTRYVNKGHIARAVLEATAWQTKEVV
                     DAMNADSGVELTSLKVDGGMVYNELLMQFQADVLGVPVIRPTVAETTALGAAYAAGLA
                     TGFWKEVEDLRANWGKDKEWDPQMAKDEVDKEYAFWKKAVTRTFDWVE"
     gene            complement(4053..4688)
                     /gene="dhaL"
                     /locus_tag="VIH70_07840"
     CDS             complement(4053..4688)
                     /gene="dhaL"
                     /locus_tag="VIH70_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEY8116310.1"
                     /translation="MSVGYEDVDRLIRAYADVIADQKDYLTQLDSAIGDADHGINMNR
                     GMQAALAKLDGQAPGDVGALFKVVGMTLVSTVGGAGGPLYGTLFLQLGTATAGKEVIE
                     AADWAAALSAAVAGVQARGKAEPQDKTMVDALIPARDAYEAALADGSTFADALRRSAA
                     AAEEGMKATIPLVARKGRASYLGERSAGHQDPGATSSWLLVKTVADTWGGD"
     gene            complement(4801..5802)
                     /gene="dhaK"
                     /locus_tag="VIH70_07845"
     CDS             complement(4801..5802)
                     /gene="dhaK"
                     /locus_tag="VIH70_07845"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY8116311.1"
                     /translation="MKKLINDPQNVVREELEGIEQAHADLVRVSYEPNFIVRADAPVQ
                     GKVALVSGGGSGHEPMHGGFVGQGMLDAACPGEVFTSPTPDQMEAATKAVNGGAGVIH
                     IVKNYTGDVMNFEMAAELCRSEGIEVESVLVDDDVAVQDSLYTAGRRGVGTTVLMEKI
                     GGAAAEEGRALKEVSDICREVNANGRSMGVALTSSTVPAAGKPTFELGDDEMEIGIGI
                     HGEPGRERMKLKPVSEIVQMMATAVVDDLPFAQGDDVLAFVNGMGGTPLIELYIVYNE
                     LAKFLKGRGISISRNLIGSYITSLEMAGCSITLLKASDDLLRLWDAPVKTAGLRWGV"
     gene            complement(5839..6570)
                     /locus_tag="VIH70_07850"
     CDS             complement(5839..6570)
                     /locus_tag="VIH70_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY8116312.1"
                     /translation="MNNNFVSEMVGTGMLILFGDGVVAAVLLHRSKAENGGWIVITTG
                     WAMAVMIGVFAATYSGGLINPAVTIGLAVAGDLEWADVPALLAGEFVGAFLGAVLVYL
                     AYLPHWRPTEDPGLKLAVYSTGPAIRNLGANMLTEIIGTFALVFGVYAIVTVGGTPEP
                     TLSAGLGPLLVAFLVWGIGLSLGGPTGYAINPARDLGPRIAHALLPIPGKGTSDWQYS
                     WVPVVGPIIGGILGAVVGSAVFANL"
     gene            6728..>7249
                     /gene="dhaL"
                     /locus_tag="VIH70_07855"
     CDS             6728..>7249
                     /gene="dhaL"
                     /locus_tag="VIH70_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEY8116313.1"
                     /translation="MSDGQELGTGFVRGWVVRTAEGVGAQRDFLTQLDAAIGDADHGV
                     NMDRGLIAAIAKLDASEDDGPGQILETVGSTLVLSVGGAAGPLYGSAFRQMGRTLGDT
                     ETFDPEDLLSALRAGLEEIQRLGAATVGDKTIVDAYSPALDAFARGLRSGQAAEEAGA
                     RARAAAEEGMRSTI"
BASE COUNT         1054 a         2593 c         2384 g         1218 t
ORIGIN      
        1 ccggcgagca gcagcgacag gacgccgagg tgcaggtaga ggtagaagac caggccgaga
       61 ccggtgatgc ggttcaccgc gaacgcccac gacccgatcg cgcggccacg aggatcgaac
      121 cacccggtcg gcgacgggcc tacgcccttg cggagcacgg gtccgcgggc cgcacgttcg
      181 gaagcgccgg ggacgccgtc ggctggtgtg gtgtcgctca tcggggcatc gctcgacggg
      241 tgagcctcgg ccccacgagc tccccccgca gctgcatgat ccgagccgcc gggttgacgt
      301 tcgacgggca cgcctcggtg cactcgaatg cggcgtggca ccgccacgcg ccgtcggggt
      361 cgtccgccca ctcgcgcacg ctcaggacgt cctcgtctcg cggctcttcg aggatccgtt
      421 gtgcccacgc gagcgccccg ggtccgacgt atcgatccgt ggtggcgacc accggacacg
      481 ctgacaggca caggccgcac tcgatgcagt cctcgtagcg ctggtaggtg gccacgccct
      541 cgggcgcctc ggccccgaga tcgaacccgc tctcgcggac gagcgggtgc gccgcgggga
      601 agcgtgcgaa gaactcggtc atgtcgacga cgaggtcggt gaggacgggg atgttcgcga
      661 ccggctcgac cgtgatctcg cgccccagat cgtggacggc ggtgacgcac atcaggacct
      721 cacgtccgtt cgcgcggacg ccgcaggtcc cgcacgaggc gtgtaggcac gagtggcgca
      781 gcgcgagcga ggggtcccgg ttgagctgga tccatcgcat ggcgtcgaac agcgtggttc
      841 gcgccgtcac cgggacgtcg aactcgtcga ggtgcggccc tcgatcgccg cgtttgaacc
      901 ggaacacgcg gaaccggcgc gtctcgctcg cctccgtcgc tgcggtggcg gtcccctcga
      961 tcgtctccat gcgcacctca ggcagcgtaa taggcctcgt gtgggagcag cacggtgtca
     1021 cgcgtttggc actccgcttc gatccccagc ttggcgcaga tcacgttggc gcacagctcc
     1081 gccatgccac ggagcgtcgt ccccttgccg cccgtgatcg tcacgaatcc ctcgacaccg
     1141 tcggtctcct tgtggtcgta cgtcttgaac gtgcgtgaga gctctcgtcc gctgtcgacc
     1201 tcgtccttcg agccgatcag cggccgtgcc gccgaccacg cggcgcggaa ctccgccgtc
     1261 ttgacggcgg ggatcagctt cgagccctcc tcgtacatgc gccggacgtg gtcctccggt
     1321 acgccgagat cgtccggatc gtcgaccgtc cacgagctgg ttccgacgat cgacagcgcg
     1381 cgctggggaa cgacgatgtc gccgtcgccc gacctgtgca tccggttgat caccatgttg
     1441 catagccgtc cgcgaagggc gagcagcacg cccggcgacg gacggatggg cacgtcgacc
     1501 ccggccattg cggcgatgcg ctctgcccat ggcccggtgg cgttcaccac gatgtcggcg
     1561 cggatctcgc cctgcttgcc ggtgacgtga tcccgcaccg ccgcgcccga gacgaggtgg
     1621 tcgtggacga acaggccctc cacctccatg tacgcgtgga tcccggcacc tccgtgcttg
     1681 gcggtcgcga agaaccgcag cggcatccgc atcgcgtcca tcgtcgcgtc ggggatccga
     1741 acggcggcct tgagctcggg gttgaggaga ggctcgagcc gaagcgcctc atcgcgtgta
     1801 agcgtctggg tcgggatgcc gcaatcggcg caccctcgga ggagcggttc gaggtactcc
     1861 atgtcttcgt cggtgatcgc gacgaacaac ccgtcgttct cctcgaagga gcccgggcag
     1921 atccggcgca ggatcgtgtt ctcctcgatg cactcgatcg ccgattcccg atcgcccacc
     1981 gcgtagcggg caccgctgtg gagaagcccg tggtgccggc ccgtcgttcc ggatgtgatc
     2041 tccccgcgtt cgacgagggt caccttgacg ccgcgaagcg tgagatcgtg cgccagggcc
     2101 ccgccggtcc cacccccgcc gacgataagg acgtgaggcc gcgaggtccg cgacgcgggc
     2161 gcgttcatcc aggccatcct cgcaccgcgg accccgaacg tccctcagtc gtccgacggc
     2221 ctctggtcct cgggcaagga cccctcctcg tgctcgtgcc cttccgggcc ctcctgcccc
     2281 tcggcgaagg acccctcgtc gtgctcgtgc tcttccgggt cctcctggcc ctcggcgaag
     2341 tcgccgtgct tgctctcctg cggatgaggg tgctcttccg cctggccttc ggcgaacgag
     2401 ccggcgtggt gctcgtgctc ctccgagtcc tcctggccct cggcgaagtc gccgtgcctg
     2461 tgctccttct ccatcgcggc ctccgatcgt ggagcgtcgc gcaatcgtct cccgattctg
     2521 gcgtttcgtt actcgaccca atcgaacgtt cgcgtgaccg ccttcttcca gaacgcgtat
     2581 tccttgtcga cctcgtcctt ggccatctgg ggatcccact ccttgtcctt gccccagttg
     2641 gcacggaggt cctcgacctc tttccagaac cccgtcgcca gaccggccgc gtaagcggct
     2701 ccgagcgcgg tggtttcggc gacggtcggc ctgatcacgg ggacgccgag cacgtcggcc
     2761 tggaactgca tcagcagctc gttgtagacc atgccgccgt cgaccttgag cgaggtgagc
     2821 tcgacgccgg agtcggcgtt catcgcgtcg acgacctcct tggtctgcca cgccgtcgcc
     2881 tcgaggaccg cgcgcgcgat gtggcccttg ttgacgtagc gcgtcaggcc ggcgatcacg
     2941 ccgcgggcat cgttcttcca gtacggcgcg aacaggccgg agaaggcggg cacgaagtac
     3001 gtgccgccgt tgtcctcgac cgtggccgcg agcgcctcga tgtcctcgga cttctcgatc
     3061 atgccgaggt tgtcccggag ccactgcacg agcgctccgg tgatcgcgat ggatccctcg
     3121 agcatgtagg tggccggctg gtcgccgatc ttgtagccga gtccggtgat caggccgctc
     3181 ttgctgggca ccggctcggt tccggtattg agcagcaaga agttgccggt cccgtacgtg
     3241 ttcttggcct cgccgaccga gtagcacgtc tggccgaaca gcgcggcctg ctggtcgccg
     3301 aggtcgcccg cgaccgggat gccggcgagg tccccccggg cctccccgta cacctcgctc
     3361 gaggccttga tctccggaag catcgccctg ggcacgccca ggatgccgag gatctcgtcg
     3421 tcccactgca gggtcgcgag atccatcatc agcgtccggc tggcgttggt gacgtcggtg
     3481 acgtgcgcac cgccgtcggg cccacccgtg aggttccaga tgcaccaggt gtcgatgttg
     3541 ccgaagagga tctcgccacc ctccgcccgc tcgcggacgc cgtcgacgtt gtcgaggatc
     3601 cacttgatct tcgggccgga gaagtacgtg gcgatgggga gtcccgtctt cggccggaac
     3661 cgatcctgtc cgccgtccga tgacagctgg ttgcagatct ggtcggtccg cgtgtcctgc
     3721 cacacgatcg cgttgtagac gggcttgccg gacgtcttct cccacacgac ggtcgtctca
     3781 cgctggttcg tgatgccgac ggcggacaga tcgctcgcct tcagaccggc cttgttcaac
     3841 gctcccgcga tgacctcctc ggagcgctgc cagatctcga gcgcgtcgtg ctcgacgtgg
     3901 ccgggcttcg gatagatctg ttcgtgttcc ttctgctcga tacccttcac ctgcccctcg
     3961 tggtcgaaga tcatgaaccg ggtgctcgtg gtgccctggt ccagcgccgc cgcgaagtcg
     4021 gccatgggcc tccctcctcc tcctccatcc cctcagtcgc cgccccaggt gtcggcgacc
     4081 gtcttcacca acagccacga ggacgtcgcg ccgggatcct ggtgcccggc gctccgctcg
     4141 ccgagatagc tcgcgcggcc cttccgggcg acgagcggga tcgtggcctt cataccctcc
     4201 tcggccgctg ccgccgaacg ccgcagggcg tcggcgaacg tcgacccatc agcgagcgcc
     4261 gcctcgtagg catcgcgcgc cgggatgagc gcatcgacca tcgtcttgtc ctgcggctcc
     4321 gccttgccgc gcgcctggac cccggcgacg gcggccgaga gcgccgcggc ccagtccgcc
     4381 gcttcgatca cctccttgcc cgccgtcgcg gtcccgagct gtaggaacag cgtgccgtac
     4441 agggggccgc cggcgccacc gacggtcgac acgagcgtca tgcccacgac cttgaacagc
     4501 gccccgacgt cgcccggcgc ctgcccgtcg agcttggcga gcgccgcctg catgccgcgg
     4561 ttcatgttga tcccgtggtc cgcatcgccg atcgctgagt cgagctgggt gaggtagtcc
     4621 ttctggtcgg cgatcacgtc cgcgtacgcg cggatcaggc ggtcgacgtc ctcgtagcca
     4681 acgctcacct ccactccctt cccgtggaac ccgtcgcttg agcgagggct ccacgagccg
     4741 agccaaccgt tggcgccggg gtgcgggccg cagcaggcga cccgcactcc gggcaaccac
     4801 tcagacgccc cagcggagtc ccgccgtctt caccggcgcg tcccacagcc ggagcagatc
     4861 gtcggaggcc ttcaggagcg tgatcgagca gccggccatc tcgagcgacg tgatgtacga
     4921 cccgatcagg ttccgagaga tcgagatccc gcgacccttc aggaacttcg cgagctcgtt
     4981 gtagacgatg tacagctcga tcagcggggt acctcccatc ccgttcacga acgccaggac
     5041 gtcgtcgccc tgcgcgaacg gcaggtcatc cacgacggcg gtcgccatca tctgcacgat
     5101 ctcggagacg ggtttcagct tcatgcgctc gcggcccggc tcgccatgga tcccgatccc
     5161 gatctccatc tcgtcgtcgc cgagttcgaa cgtcggcttg cctgccgccg gaaccgtgga
     5221 cgaggtgagc gccacgccca tgctgcgacc gttcgcgttc acctcccgac agatgtcgga
     5281 gacctccttc agggcgcggc cctcctccgc ggccgctccc ccgatcttct ccatcaggac
     5341 cgtcgtgccg accccgcgcc gccctgccgt gtagaggctg tcctgcacgg cgacgtcgtc
     5401 gtccaccaga acagactcga cctcgatgcc ctccgatcgg cagagctccg ccgccatctc
     5461 gaagttcatg acgtcgcccg tgtagttctt gacgatgtgg atcacgccgg cgccgccgtt
     5521 gaccgccttc gtcgcggcct ccatctgatc gggggtcggt gaggtgaaga cctcgcccgg
     5581 acacgcggcg tccagcatcc cctgcccgac gaacccgccg tgcatcggct cgtggcccga
     5641 accgcctccg gagacgagcg caaccttgcc ctgcaccggg gcatcggccc ggacgatgaa
     5701 gttgggctcg tacgagaccc gaacgaggtc cgcgtgcgcc tgctcgatgc cctccaactc
     5761 ctcccggacc acgttctgcg gatcgttgat cagctttttc atcgcactcc ttccctcgcg
     5821 gaatcctcct cgatcctcct acaggttcgc gaacacggcc gagccgacga cggcgccgag
     5881 gatcccgccg atgatcggcc cgacgaccgg gacccagctg tactgccagt cggacgtgcc
     5941 cttgcccggg atcggcagca gggcgtgggc gatccgtggg cccaggtcgc gcgcggggtt
     6001 gatcgcgtac ccggtcggcc caccaaggga caggccgata ccccacacga ggaacgcgac
     6061 gagcagcggg ccgaggccgg ccgagagcgt gggctcgggg gtgccgccga ccgtgacgat
     6121 cgcgtagacc ccgaacacca gcgcgaacgt cccgatgatc tccgtcagca tgttcgcgcc
     6181 gaggttacgg atggccgggc cggtgctgta gacggcgagc ttgagccccg ggtcctcggt
     6241 cggacgccag tggggcaggt acgcgaggta cacgagcacg gctccgagga acgccccgac
     6301 gaactctcct gcgagcaagg ccggcacgtc ggcccactcg aggtctccgg ccaccgcgag
     6361 gccgatcgtc acggccgggt tgatgaggcc gccgctgtac gtggccgcga agacaccgat
     6421 catcacggcc atcgcccatc ccgtcgtgat gacgatccat ccgccgttct ccgccttcga
     6481 gcggtggagg agcaccgccg ccaccacgcc gtcgccgaag aggatcagca tcccggtgcc
     6541 gaccatctcg gacacgaagt tgttgttcat gccttccccc ctcaggcgcc acggcggcac
     6601 cgaatcccat cagaactccg ttcggtggtc tgagtcaaat cctgaacatc gccttggcca
     6661 gcacgagatc tcggcggagc gttcggtatg gtcgaacaca cagcgattgg ggcgttagga
     6721 tgcgggggtg agcgacgggc aggagctcgg aaccgggttc gtgcgcgggt gggtcgtgcg
     6781 gacggccgag ggcgtgggcg cccagcgcga cttcctcact cagctcgacg ccgccatcgg
     6841 ggacgccgac cacggcgtga acatggatcg tggcctcatc gccgcgatcg ccaagctcga
     6901 cgcctcggaa gacgacggcc ccggccagat cctggagacc gtgggaagca cgctggtcct
     6961 gtccgtgggt ggagcggcgg gacccctgta cggctcggcg ttccggcaga tgggaaggac
     7021 cctcggtgac accgagacct tcgacccgga ggatctgctg tctgcgctga gggccgggct
     7081 cgaggagatc cagcggctcg gggcggccac ggtgggcgac aagacgatcg tcgacgccta
     7141 ctcgccggcg ctcgacgcgt tcgcacgtgg gctgcgctcg ggccaggcgg ccgaggaggc
     7201 cggggcccgg gctcgagcgg ccgccgagga gggcatgcgc tcgacgatc
//
LOCUS       DATBRK010000260         4372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_478144, whole genome shotgun sequence.
ACCESSION   DATBRK010000260 DATBRK010000000
VERSION     DATBRK010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478144"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VIH70_07860"
     CDS             <1..709
                     /locus_tag="VIH70_07860"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY8116314.1"
                     /translation="IEALKHGGFHHGVDVQIDWVSSDDVDEGDVDEMLRDVHGIVVPG
                     GFGWRGVEGKLEVVRYARERGVPFLGLCLGLQCAVIEFARSVCGLEGANSSEFDPATP
                     HPVIDLLPEQKDVTDLGGSMRLGAQPCHVVPGTRAEQVYGEPVVYERHRHRYEVNPAY
                     HEALSGRGLVFSGLSPDGRLVEIIELQDHPFFMAGQFHPELRSRPTRPHPLFREFVGA
                     SKTFSAAGSERTVALPG"
     gene            744..2081
                     /locus_tag="VIH70_07865"
     CDS             744..2081
                     /locus_tag="VIH70_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8116315.1"
                     /translation="MERSETRYARSGDLSIAYQVIGEGPFDLVYVPGWVSNVELMWDE
                     PDYAAFLERLASFSRLIIFDKRGTGLSDPVPLEQLPTLEHRMDDVRAVMDAVGSERAA
                     LLGHSEGGNMCVLFSATYPDRTTALVLVGCYAKRIRSDDYPWAPAPKDREREIEGTET
                     RWGSPDMLRALAPSKASDEAFERWVGRYLRQSASPKAAAALLRMNTQIDVRDVLPAIR
                     VPTILIYRTDDADVHVDEGRYIAERIPGARFVELPGADHTMWTGDATAILDEIEEFLT
                     GVRRGPEPDRVLATVLFTDVVGSTELATRLGDRAWRDLLGRHHAAVRRELERWRGREV
                     DTAGGGFLATFDGPARAIRCAVAAVDAVRGLGMEIRAGVHTGEVEVSNGDLRGIAVHI
                     GSRVSGIAGPSEVLVSRTVADLVAGSGIVLTERGEHELKGVPGTWLVYAVESA"
     gene            2146..3270
                     /gene="ald"
                     /locus_tag="VIH70_07870"
     CDS             2146..3270
                     /gene="ald"
                     /locus_tag="VIH70_07870"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY8116316.1"
                     /translation="MIVGVPKEVKDNEYRVSMTPEGARELTAAGHQVLVQDGAGAGSS
                     IPQQRYERAGAEIVATAAEVWSSSDMIMKVKEPIASEFEHLREGQILFTYLHLAAGRE
                     LTHALAERKVSAVAYETVQLPDGRLPLLAPMSEIAGRMAPHVAARLLEKEYGGRGILM
                     GGVSGVRPAKIFVLGAGMAGSNAAWIAAGMEAEVIVVDTNLDRLRFIDQIHKGRIITL
                     MSDRLTLEQRVREADVVIGSVLVPGARAPKLVTEDMIRSMRTGAVAIDIAIDQGGCME
                     TSRMTTHSDPTYVVDGVVHYCVGNMPGAVPNTSTYALTNVTLPYALQIAVRGLEDAVR
                     ADAALGLGVNVIAGEVTNPGVAEAHTMRFTPLSAIVDGLS"
     gene            3288..4232
                     /gene="xerD"
                     /locus_tag="VIH70_07875"
     CDS             3288..4232
                     /gene="xerD"
                     /locus_tag="VIH70_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018812700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY8116317.1"
                     /translation="MQARDALAKQIQRFLDHLTIERGLSGHTVVAYRRDLERYATFLR
                     ARGITDARRVDERAVAAHVAAVSASTYGDGKRYRATSVVRALSSIRVFHRFLLRDGKI
                     SSDPTAAAVRPKLPRSLPKPLSVDDVARVLAHPDRSAKGLRDRAVLETLYGAGLRISE
                     LVGLDVDDLDLQEGSVRVLGKGGKERDVPIGRYARDAISAYLTRVRPQIVTARSRSAL
                     FLNLRGGRLTRQGCTGIVQQHAAGAGIHKRVSPHTFRHSFATHLLEGGADVRVVQELL
                     GHASVATTQVYTLVTREHLREVYFTSHPRARATRRDSA"
BASE COUNT          638 a         1485 c         1614 g          635 t
ORIGIN      
        1 gatcgaggcg ctcaagcacg gcgggttcca ccacggcgtc gacgtccaga tcgactgggt
       61 gtcctccgac gacgtcgacg agggcgacgt cgacgagatg ctgcgcgacg tccacggcat
      121 cgtcgtgccg ggcgggttcg ggtggcgggg ggtcgagggc aagctcgagg tcgtccggta
      181 cgcgcgcgag cgcggcgtgc cgttcctcgg actctgcctc gggctccagt gcgcggtgat
      241 cgagttcgcg cggagcgtct gcggcctcga gggcgcgaac tcctccgagt tcgacccggc
      301 tacgccgcac ccggtgatcg acctgctccc cgagcagaag gacgtgacgg atctcggcgg
      361 ctcgatgcgc ctgggggctc agccgtgcca cgtcgtgccg gggacccgtg ccgagcaggt
      421 ctacggcgag cccgtcgtgt acgaacgcca ccgccatcgg tacgaggtca accccgcgta
      481 ccacgaggcg ctctccggca ggggcctggt gttctcgggc ctgtcgccgg acgggcgcct
      541 ggtcgagatc atcgagctcc aggaccaccc gttcttcatg gcgggccagt tccaccccga
      601 gctgcggtcg cgaccgacgc gaccgcaccc gctgttccgc gagttcgtcg gcgcctcgaa
      661 gacgttctcc gcggcgggat ccgagcggac ggtcgccctg cccggctagg ctccacctgc
      721 tcggctgggg agcgagggtc ggcgtggagc gttcggagac cagatacgcg cgcagcggcg
      781 atctgagcat cgcctatcag gtgatcggcg agggaccatt cgacctcgtg tacgtgccgg
      841 ggtgggtctc gaacgtcgag ctgatgtggg acgagcccga ctacgccgcc ttcctcgagc
      901 gactcgcgtc gttctcgcgg ctgatcatct tcgacaagcg cgggacgggc ctgtcggacc
      961 cggtgccgct cgagcagttg ccgacactcg agcaccggat ggacgacgtg cgcgcggtga
     1021 tggatgcggt ggggtcggaa cgcgcggcgc tcctgggtca ttccgagggc ggcaacatgt
     1081 gcgtcctgtt ctcggcgacg taccccgatc gaacgaccgc gctggtcctg gtcggctgtt
     1141 acgcgaagcg gatccgttcg gacgactatc cctgggcccc cgcgccgaag gatcgcgagc
     1201 gcgagatcga ggggaccgag accaggtggg gatcgcccga catgctccgg gcgctcgcgc
     1261 cgagcaaggc gagcgacgag gccttcgagc gatgggtcgg ccggtacctc cgccagtccg
     1321 ccagccccaa ggcggccgcg gccctgcttc gcatgaacac acagatcgac gtccgcgatg
     1381 tcctgcccgc gatccgggtt ccgacgatcc ttatctaccg gacggacgac gccgacgtgc
     1441 acgtcgacga gggccggtac atcgcggaga ggatccccgg cgctcggttc gtcgagctgc
     1501 cgggagccga tcacacgatg tggacggggg atgccacggc gatcctggac gagatcgagg
     1561 agttcctgac cggcgtccgg cgcggacccg agcccgatcg cgtgctcgcg acggttctgt
     1621 tcaccgacgt cgtcggttcc accgagctcg cgactcgtct gggcgaccgc gcctggcggg
     1681 acctgctcgg ccggcaccac gcggccgtcc gacgcgagct cgagcgctgg cggggccggg
     1741 aggtggacac cgccggcggc ggcttccttg cgaccttcga cggtcccgcc agggcgatcc
     1801 ggtgcgcggt cgcggcggtc gacgcggtcc ggggactcgg gatggagatc cgggccggcg
     1861 tccacacggg tgaggtcgag gtctcgaacg gcgaccttcg gggcatcgcg gtccacatcg
     1921 gctcgcgcgt ctcgggcatc gccggcccga gcgaggttct ggtctcgcgc acggtcgccg
     1981 acctcgtggc cggttccggc atcgtgctga ccgaacgcgg ggagcacgag ctgaaggggg
     2041 ttcccggcac ctggctcgtg tacgcggtgg agtcggcctg agggaccggt atcgccgccg
     2101 ggtcgggtac gcttcccgcg tcccgtacgt gggaagggcc agaccgtgat cgtcggggtc
     2161 cccaaagagg tcaaggacaa cgagtaccgg gtctctatga cgcccgaggg ggctcgcgag
     2221 ctcaccgccg ccgggcacca ggtcctcgtc caggacggcg cgggagccgg ctcgagcatc
     2281 ccgcagcagc ggtacgagcg cgctggcgca gagatcgttg cgacggcggc cgaggtctgg
     2341 tcgagctccg acatgatcat gaaggtgaag gagccgatcg catcggagtt cgagcacctg
     2401 cgcgagggcc agatcctgtt cacctacctc cacctggcgg ccggccggga gctcacacac
     2461 gcgctggcag agcgcaaggt ctcggcggtc gcgtacgaga ccgtgcagct gcccgacggc
     2521 cgcctgcccc tgctggcgcc gatgagcgag atcgcgggcc ggatggcgcc ccacgtcgcg
     2581 gcgcgcctcc tggagaagga gtacggcggc cgcgggatcc tgatgggtgg cgtctcgggc
     2641 gttcggccgg ccaagatctt cgttctcggc gccggcatgg cggggtcgaa cgcggcgtgg
     2701 atcgccgccg gcatggaggc cgaggtgatc gtggtcgaca cgaacctgga ccggctccgg
     2761 ttcatcgacc agatccacaa ggggcggatc atcacgctga tgtcggaccg actcacgctc
     2821 gagcagcgcg tgcgtgaggc cgacgtggtg atcgggtcgg tgctcgtgcc gggcgcccgg
     2881 gccccgaagc tggtcaccga ggacatgatc cgttcgatgc gcaccggcgc ggtcgcgatc
     2941 gacatcgcga tcgaccaggg cggctgcatg gagacgtcgc ggatgaccac gcactcggat
     3001 ccgacctacg tcgtggacgg cgtcgtgcac tactgcgtcg gcaacatgcc gggcgccgtg
     3061 ccgaacacct cgacgtacgc gctcacgaac gtgacgctgc cgtacgcgct gcagatcgcc
     3121 gtgcggggtc tggaggacgc ggtgcgcgcc gacgccgccc tcgggctcgg cgtcaacgtg
     3181 atcgcgggcg aggtcaccaa cccgggggtc gccgaggccc acacgatgag gttcacgccg
     3241 ctgtccgcga tcgtcgacgg gctgagctga gcgtccagga cgggcccatg caggctcgag
     3301 acgcgctcgc caagcagatc caacgcttcc tcgatcacct gacgatcgaa cgcggtctgt
     3361 cgggccacac ggtggtcgcc taccgtcgcg acctcgagcg ctacgccacc ttcctgcgcg
     3421 cccgagggat cacggacgcc cgtcgcgtcg acgagcgggc ggtggccgcg cacgtcgcgg
     3481 ccgtctcggc ctcgacgtac ggagacggga agcgctaccg ggcgacgtcg gtggtgcggg
     3541 cgctgtcctc gatccgggtg ttccaccggt tcctgctgcg ggacgggaag atctcgagcg
     3601 accccacggc ggcggcggtc cgtccgaagc tcccgcgttc gcttcccaag ccgctgtccg
     3661 tggacgacgt ggcccgcgtg ctcgcgcacc ccgatcgctc ggcgaagggg ctgcgggacc
     3721 gcgcggtcct cgagacgctg tacggggccg gcctgcggat ctccgagctc gtcggcctcg
     3781 acgtggacga cctggacctg caggagggga gcgtccgggt tctcggcaag ggcggcaagg
     3841 agcgggacgt gccgatcggc cggtacgcgc gcgatgcgat ctcggcgtac ctgaccaggg
     3901 tccggccgca gatcgtgacc gctcgttcgc gttcggcgct gttcctgaac ctgcgcgggg
     3961 gacgcctgac ccgccaggga tgcacgggga tcgtccagca gcacgccgcc ggcgccggca
     4021 tccacaagag ggtcagcccg cacacgttcc ggcactcgtt cgcgacccac ctgctcgagg
     4081 ggggcgcgga cgtgcgggtc gtccaggagc tgctcggaca cgcgagcgtg gccaccaccc
     4141 aggtctatac gctggtcacc agggagcacc tgcgggaggt ctacttcacg tcccacccgc
     4201 gggcgcgcgc gacgaggagg gactccgcgt gatcggaccg gacaagctcg ccgagctgcg
     4261 cgaggccctc gaggagcagc gcgagaacct gcgcaaggag atcgcggagc aagggggcga
     4321 cccggactcg gacgacgccg cgatcgacgt cgaacgcggg ttcgccgaca gc
//
LOCUS       DATBRK010000261         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2206384, whole genome shotgun sequence.
ACCESSION   DATBRK010000261 DATBRK010000000
VERSION     DATBRK010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206384"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VIH70_07880"
     CDS             <1..512
                     /locus_tag="VIH70_07880"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY8116318.1"
                     /translation="DAAALCDEARGEDVLLVANENSPQQVVISGSVAAIERAEAIAAE
                     RRLRAVRLSVAGAFHSPLMEPATEPLSAAISEAAFATPRFPIASNVTGTLVTDPAELR
                     ALAERQVVSPVRWEASLGALRELGADTFVEAGPGDVLTKLAKRAAPGARAVAVGSPET
                     ASALAREHA"
     gene            633..1637
                     /locus_tag="VIH70_07885"
     CDS             633..1637
                     /locus_tag="VIH70_07885"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY8116319.1"
                     /translation="MSAQRHAAIAGLGSVLPERIVSNEWFEAFIETSDEWIRERTGIR
                     ERHFAADGQTTSDLATDAATRALEAASIAPEQLDLLVCATLTGDTPIPSTAVWVQRKM
                     GIAAPAFDVNAACAGFSYALSTATAFIESGQAETVLVMGAEVLSRVMNFHDRTTCVLF
                     GDGAGAMVLRRSEEPGVLGSALGADGRAAEILIIPGGGSAEPTSHASVDANDHTIQMP
                     NGREVFKRAVVEMASACRQLLEKSGFVPEDVDLLIPHQANARIMIAVAERLKIPMDRA
                     VVDVETVGNTSAASIPVALDRAWRGGRIREGDLVLLTSFGAGLAWGANLIRWTGPAGP
                     "
     gene            1640..2380
                     /locus_tag="VIH70_07890"
     CDS             1640..2380
                     /locus_tag="VIH70_07890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEY8116320.1"
                     /translation="MPSTKVAVVTGGSRGIGRACAEALARDGWSVAIGYRSSEADAKE
                     TLASLETIGTPGLAVAMDVTEEGSVAEAFRRVDDELGPVTGLVNNAGMSRDGLLLKYP
                     MDVYERTMATNLRGAFLCCRSALRGMLRAKWGRIVNMSSAVALHGNAGQTVYAATKAG
                     LVGLTRSLAREVGVKGITVNALCPGLLDTDMTSHLTEPARAYYLDQTPVGRAAHLEEV
                     ASVVRFLMSEEASYVNGAVLPVDGGLTA"
     gene            2535..>2726
                     /gene="acpP"
                     /locus_tag="VIH70_07895"
     CDS             2535..>2726
                     /gene="acpP"
                     /locus_tag="VIH70_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8116321.1"
                     /translation="MDKAEIESRVRKVLAEQLAVDEAQVVPDARFAEDLNADSLDLVE
                     AVLALEEEWSIDIPEEEMEG"
BASE COUNT          427 a          932 c         1008 g          359 t
ORIGIN      
        1 cggacgcggc ggcgctgtgc gacgaagctc ggggcgagga cgtgttgctc gtcgcgaacg
       61 agaattcgcc gcagcaggtg gtgatcagcg gcagcgtcgc ggcgatcgaa cgcgcggagg
      121 ccatcgccgc cgaacggagg ctcagggccg tgcgcctgag cgtcgcgggg gcgttccact
      181 cgccgttgat ggagcccgcg accgagcccc tgtcggccgc gatctcggag gccgcgttcg
      241 cgacgccacg gttcccgatc gcctcgaacg tcacggggac cctcgtcacc gaccccgccg
      301 agctccgcgc cctcgccgag cgccaggtgg tctcgcccgt ccggtgggag gcgagcctcg
      361 gcgccctccg cgagctgggg gccgacacgt tcgtcgaggc gggaccgggc gacgtgctca
      421 ccaagctcgc gaagcgagcc gcgcccgggg cacgggccgt cgcggtcggc tcgcccgaga
      481 ccgcgtccgc actcgcccgg gaacacgcct gacgcccggc cgggccgcgc ccggccgggc
      541 atcgatgact cgtggcgccg cgtcgcgcac catccccggg cgatatcctc gaccatcgac
      601 gtgggcggcg accgtcgccc cgagggggga tcgtgagcgc gcagcgtcac gccgccatcg
      661 ccgggctggg ctcggtcctg ccggagcgga tcgtgtcgaa cgagtggttc gaggcgttca
      721 tcgagaccag cgacgagtgg atcagagaac gcaccgggat ccgagagcgg catttcgccg
      781 cggacgggca gaccacgtcc gacctcgcca ccgacgccgc gacgcgagcg ctcgaggcgg
      841 cgtcgatcgc gccggagcag ctggacctcc tcgtctgcgc gacgctgacc ggggacaccc
      901 cgatcccctc caccgccgtg tgggtgcaac gcaagatggg gatcgcggcg cccgcgttcg
      961 acgtgaacgc ggcgtgtgcg gggttctcct acgccttgtc gacggcgacg gcattcatcg
     1021 agtccggcca ggccgagacc gtgctcgtga tgggcgccga ggtcctctcc cgtgtgatga
     1081 acttccacga ccgcaccacc tgcgtgctgt tcggcgacgg cgcgggcgcc atggtgctgc
     1141 ggcgttcgga ggagcccggg gtgctcggat cggcgctcgg cgccgacgga cgcgccgcgg
     1201 agatcctgat catccccggg ggcggctccg ccgagcccac gagtcatgcg tcggtcgacg
     1261 cgaacgacca cacgatccag atgccgaacg gtcgcgaggt cttcaagcga gcggtcgtcg
     1321 agatggccag cgcgtgccgc cagctgctcg agaagtcggg cttcgtcccg gaggacgtcg
     1381 accttctgat cccccaccag gcgaacgcgc ggatcatgat cgcggtggcc gaacgcctga
     1441 agatcccgat ggatcgcgcg gtcgtcgacg tcgagaccgt cgggaacacg tccgcggcct
     1501 cgatcccggt cgccctcgat cgcgcgtggc gcgggggtcg gatccgcgag ggcgaccttg
     1561 tgctgctgac atcgttcggc gccgggctcg cgtggggcgc gaacctgatc cgctggacgg
     1621 gaccggcggg accctgaccg tgccctctac caaggtcgcc gtcgtcacgg gtggttcgcg
     1681 cggcatcggc cgcgcgtgcg cggaggcgct cgcgcgcgat ggctggagcg tggcgatcgg
     1741 ctaccggtcg agcgaggccg acgccaagga gacgctcgcg agcctcgaga cgatcggcac
     1801 gccgggtctc gcggtcgcga tggacgtcac ggaggagggt tcggtcgccg aggcgttccg
     1861 ccgcgtggac gacgagctgg gccccgtgac gggcctggtg aacaacgccg ggatgtcgcg
     1921 ggacgggctc ctcctcaagt acccgatgga cgtctacgag cggacgatgg cgaccaacct
     1981 ccggggcgcg ttcctgtgct gtcggtccgc gctgcggggg atgctccgag ccaagtgggg
     2041 gcggatcgtg aacatgtcct cggcggtcgc cctgcacggc aacgcgggtc agaccgtcta
     2101 cgcggccacg aaggccggac tcgtcgggct gacgcggtcg ctcgcccgcg aggtcggagt
     2161 caagggcatc acggtgaacg ccctgtgccc ggggctgctc gacacggaca tgacctccca
     2221 cctgaccgag cccgcgcgcg cgtactacct ggaccagacg ccggtgggtc gagcggcgca
     2281 cctcgaggag gtcgcgagcg tcgtgcgatt cctgatgtcg gaggaggcct cgtacgtgaa
     2341 cggtgcggtc ctccccgtgg acggcgggct gaccgcctag tcgaaggacg gccgcccccc
     2401 gtagcatgga ccggtccccg aaacggacgc ccggggacca gcacgaagag cagcagagca
     2461 acagagcaac agacaaagac acggtcaaaa ggacacagcc aaaaggacac agccaaaagg
     2521 acacaggagg aaggatggac aaggcagaga tcgagtctcg ggtccgcaag gtcctcgcgg
     2581 agcagctcgc ggtcgacgag gcccaggtcg tgcccgacgc ccgtttcgcc gaggacctga
     2641 acgccgactc gctcgacctg gtcgaggcgg tactcgccct cgaggaggag tggagcatcg
     2701 acatccccga ggaagagatg gaaggg
//
LOCUS       DATBRK010000262         3728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1487741, whole genome shotgun sequence.
ACCESSION   DATBRK010000262 DATBRK010000000
VERSION     DATBRK010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487741"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VIH70_07900"
     CDS             complement(<1..425)
                     /locus_tag="VIH70_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY8116322.1"
                     /translation="MSQPRLRAVPDLVESASGLDEPLSFDTFFAAESQTLFRRMWLVT
                     RDRHEAEEIVQDAFLSLYERWDRAADWDDPTSYLYRTAFNTWKKRTRRAARAVRHGFS
                     SDPGDEFEAADARTVVGAALARLSPRQRAAVVLTELLGFS"
     gene            complement(928..1056)
                     /locus_tag="VIH70_07905"
     CDS             complement(928..1056)
                     /locus_tag="VIH70_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116323.1"
                     /translation="MRAAIFHDPKDISVGTRPDPVIQEPTDAVVRVVLGSVCGSDL"
     gene            complement(1205..2725)
                     /locus_tag="VIH70_07910"
     CDS             complement(1205..2725)
                     /locus_tag="VIH70_07910"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEY8116324.1"
                     /translation="MATKTVERERERKFDAHEDVQIPHLPGTSVTHAGKVRLTATYWD
                     TVNRRLLRWGHTLRHRKASDGSEDRWTLKLATPAKRKKKDALVRAEVHAAGSALFPPT
                     MLRRLARAVVRRDLLTPIAVVRTQRRRLEVADQGSMDRIEVDHDRVTSVVGLRRGLAF
                     HQIEVEAASPDADRLMDQMSDALIEAGATPTDRAKIAEVLGDDAPEPEIVLPPSGPKI
                     SIRDLVRTAIGAGATRLVANDPAARLGSDPEAIHQARVATRRLRSDLKTLEPLLDPAA
                     AGWMRDELSWVGELLGSVRDIDVLIDGIEELATTRGLEPRAVSAIVKELEKDRHRRHA
                     ELLDGLDSRRYLALVNALIDSGGAPPLADEVDGERRARRPVRKLVRKTWRRTARAVAR
                     LDGDPSDEDLHEIRKRAKRARYAAELATAALHEEADPLADRLADLQDVLGELQDAVVA
                     QERLTMLVRRGRLTIEAAFLAGKLVCLEDEARSDARDRWSAAWESARPKRLRNWLS"
BASE COUNT          643 a         1287 c         1172 g          626 t
ORIGIN      
        1 gagaacccga ggagctcagt caaaaccacg gccgctcgct gacgcggcga gaggcgagcc
       61 agggcggcac ccacgacggt ccgggcgtcg gcggcctcga actcgtcccc cggatcggag
      121 gagaagccgt gtctcacggc acgagcggct cgtctcgtcc gtttcttcca cgtgttgaag
      181 gccgttcgat agaggtagga agttggatcg tcccagtcgg ccgcacgatc ccaccgctcg
      241 tacaggctca gaaaggcgtc ctgcacgatc tcctccgctt catggcgatc gcgggtcacg
      301 agccacatcc ggcggaacag cgtctggctc tcggccgcga agaatgtgtc gaacgacagc
      361 ggctcgtcga gcccggacgc gctctccact agatcgggca cggccctcag tcggggttgc
      421 gacatctcat ccaaggagcc cttaccggcc ggaaacgttt aggcgatcct acgtttcggc
      481 tccgagcctc cgcgcggcaa ggaccgcgca gaggcccacg gccccatctc ccacgacgcc
      541 gaccacgctt ccttcgtgca cgccggcgct cacggccacg tggtggcccg tggtcatcac
      601 atcggagaga gcgccacgag tgatcgcatc gtctcgtgcg agtaaccgat gcccgggacc
      661 cgaacgaagg tgctgcgcgc gaatgggact cgaacggcct ctccctgacc tccgtcgatc
      721 ccgtggttcc cgaacgagcc tctggcgaca cagtgtgacg tcaccccgca cggcagttcg
      781 cgcacgtccc gtcgcaaata ccgtgaatgg cgcgacgacg aggtcgccct cggcgaaccc
      841 ttgcacctcg gcgccgatct ggtccaccac accgatgaac tcgtggccga tcggtcccag
      901 cgcgtgcgga gtctccccgc ggtgttatca gagatcggag ccgcagacgg accccagcac
      961 gacgcggacg acggcatcgg tcggctcttg gatgaccggg tctgggcgtg tgccgacgct
     1021 gatgtccttc ggatcatgga agatcgccgc ccgcatcaga tgaaatcctt tcgatggcta
     1081 ctccatcgtc ctacccaacc agccttcatg gacagcggga gactcgccgc ctcggccggg
     1141 agcccgcgcc gcacggcgat cccggcgtcc agaacggcga aaaaccgacc agccggcaaa
     1201 gcaatcagct gagccagtta cggagacgct tgggccgcgc cgattcccat gcggccgacc
     1261 aacgatcacg tgcgtccgag cgagcctcgt cctcgaggca gacgagcttc ccggcaagga
     1321 aggccgcctc gatcgtgagc cgaccgcggc gaaccagcat ggtcagccgc tcctgagcga
     1381 cgaccgcgtc ctgcaattca cccaggacgt cctgcagatc ggcgagccga tcggccagag
     1441 ggtccgcttc ctcgtgaagg gcagcggtcg ccagttccgc agcgtaccga gcccgtttcg
     1501 cacgcttgcg gatctcgtgg aggtcctcat cggacgggtc tccatccagg cgcgccactg
     1561 cgcgcgccgt ccgccgccac gtcttccgta cgagcttgcg gacaggacgc ctcgcccgcc
     1621 gttcgccgtc cacctcgtcc gccagcggag gcgcgccgcc ggaatcgatc aaggcattga
     1681 ctagcgcgag gtatcgccgc gagtcaagac cgtccagcag ctcggcgtga cgccgatgtc
     1741 gatccttctc gagctccttc acgatcgcgg atacggccct cggctcgaga ccgcgtgtgg
     1801 tcgcgagctc ctcgatcccg tcgatcagca cgtcgatgtc tcggaccgag cccaagagct
     1861 ctccaaccca ggagagctca tccctcatcc atccggctgc cgccggatcc agcagcggtt
     1921 ccagcgtctt gagatccgat cggagccgtc gcgtggcgac ccgcgcctga tggatcgcct
     1981 cggggtcgga gccgagcctt gccgcgggat cgttcgcgac caggcgggtc gcacccgccc
     2041 cgatcgcggt ccggaccagg tcgcggatcg agatcttcgg accggacggg ggaaggacga
     2101 tctccggctc cggggcgtcg tctccgagca cctccgcgat cttcgcccga tcagtagggg
     2161 tcgcccctgc ctcgatcagg gcgtccgaca tctggtccat gaggcgatca gcatccggcg
     2221 aggcagcttc gacctcgatc tggtggaacg cgagtcctcg ccggagacca acgacggacg
     2281 tgacgcgatc gtggtcgacc tcgatccgat ccatcgatcc ctggtcggcg acctccaacc
     2341 ggcgacgttg cgtacggacg acggcgatcg gcgtgagcag gtccctacgc acgacggctc
     2401 gggccagccg acggagcata gtcgggggga acagggcaga gcccgccgcg tgaacctccg
     2461 cccggacgag tgcatccttc ttcttccgtt tcgccggggt cgcgagcttg agggtccagc
     2521 gatcctcgga cccgtcggaa gccttgcgat gacgcaacgt gtggccccac cggagcagtc
     2581 gccggttgac cgtgtcccag taggttgcgg tcagccgaac ctttccggca tgagtcaccg
     2641 atgtgccggg taagtgcggg atctgcacgt cctcgtgagc gtcgaacttg cgttcacgct
     2701 cacgctcgac cgtcttggtc gccacgaact ctccgtaccc atcttgcacc gacgaaaatc
     2761 tcggccttga gagctcgtca ctcgtgccgg ccggacggtt cgagatcgtg tatgcaaaat
     2821 cccaagtagg ccaacagccc atccacgacc ctggaaccgt gcgcctcgtg ccgcctgctc
     2881 aggaatgatg tggcttccca ctaacccacg aacgcggtac gaaactcgac ccaccacgag
     2941 gccaacgcca aagtcgcgca cgtgtccaac cgctctgaaa cgggtaggga ccacaagcgc
     3001 ggaagacgag ccgaaggagg tccgtcatgg acaactcgag gagcatgcag ccgcgcagtg
     3061 gttggacctg gaaggtatgg gtcggaatcg ttgtcggcct agcggtcgtt gcccggactc
     3121 atcgttctca tcgccgcgaa tgccggcgga ggcccgggtg gtggtggctc gggcgggatc
     3181 tactgaccca acgtttcagc caaatcgtgt cggtcgtggg ggtccttgaa cctccgcggg
     3241 ttccgatgcg catgcacgcg gtcgtgtcgt gggacccgca cggatgatcg cgtgatcgat
     3301 ccatggctcg gacgggggcg ttcgggttct tacagttccc ttcgcccctg ccgtggagca
     3361 caaacgcgct cggtgagtgt cgtcccgttc gttccgattc gccctggacg cacgcctctg
     3421 acctgcgcaa acggccctcc cgacgatccg agagggccgt agttgtggcc aggttgtggc
     3481 cagcgctacc cgaacaacgt gtcggcggca tccgcggccg cccgcccgac tgacgggatc
     3541 acggccgagt agacgtccat cgtgaacgcg acgtgatgtc cgtacctgcg ttggcaccca
     3601 cgctgccgcc gatcaggccg agtaggcccg ctgctgctac cactggcatg agcctgtttc
     3661 tcatttcgtc ttctccattt ctctcaaggg aaaaggaccg gggccgaagc cccggtccta
     3721 atccgttc
//
LOCUS       DATBRK010000263         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1200139, whole genome shotgun sequence.
ACCESSION   DATBRK010000263 DATBRK010000000
VERSION     DATBRK010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200139"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..751
                     /locus_tag="VIH70_07915"
     CDS             197..751
                     /locus_tag="VIH70_07915"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEY8116325.1"
                     /translation="MAETPDDILRALADPERLTIAGHLARSDAASAELADALDLPVPR
                     LRRHLSRLVAAGVVRLKDDRRTYRLDAETLRRATEQVGPPRDAGLALGAASDDEEAVL
                     RAFFRDGRLTEVPAKESKRRIVLGRIALEFEPGVDYDEATVNVIVGRFSTDHAALHRY
                     LVDEGFLDRDHGRYWRTGGRVDLS"
     gene            820..2406
                     /locus_tag="VIH70_07920"
     CDS             820..2406
                     /locus_tag="VIH70_07920"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015233264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY8116326.1"
                     /translation="MAKTQDLPQPSVRTVEDRIDELRKRRAALLDGSRKDAVRKQHEK
                     GKLTARERVELLVDRDSFQETDPFAVHRSHDFGIERNRPAGDGAITGYGTVDGRRVFV
                     ASQDFTVFGGSLGEIHAQKICKVQDLAMSTGAPFIQINDSGGARIQEGAASLAGYGYV
                     FERNVRASGVIPQISVIMGPCAGGAVYSPAITDFTFMVRETSHMFITGPDVIKAVTGE
                     EVTFEELGGAMTHASRSGVASFVSQDEEECLAMVRHLLSYLPSNNLEDAPAFAPVDDP
                     DRHDEGLTHVIPDSAREPYDMHEVIRRIVDDGDFFEVFPFWAMNVVTGFARLDGRAVG
                     VVANQPKVLAGTLNIDASEKAARFVRTCDAFNIPIVTFVDVPGFLPGTDQEYQGIIRH
                     GAKLLYAFTEATVPRLTVITRKAYGGAYLVMNSKHLRADVSFAWPTAEIAVMGPEGAV
                     NVVFRKQIEKAEDPVAARAELIQQYQDKFATPYIAAERGFIDDVIEPAETRPRLIRAL
                     RMLSTKREIVPARKHGNIPL"
     gene            2438..2698
                     /locus_tag="VIH70_07925"
     CDS             2438..2698
                     /locus_tag="VIH70_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116327.1"
                     /translation="MSDDRRFELPDDLSPDEERAIIRALERYFAQESPHPHPWVLAGR
                     IDATGFGALQARHHADAPWRRFVDVPFARRGVPPQAGRGDAG"
     gene            2699..>2889
                     /locus_tag="VIH70_07930"
     CDS             2699..>2889
                     /locus_tag="VIH70_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116328.1"
                     /translation="MTAAKTASAVGGRDAALEAFRAEGCSAPRSWGNGPGDAYGPHAH
                     GFHKVLFCLGGSITFHLDDG"
BASE COUNT          453 a         1024 c         1017 g          395 t
ORIGIN      
        1 cttgccgagc cacagcggct ccggcggctc gaacgcgcgg gccgcctcct cgatcctgtc
       61 gcgcacccga ggcaccgcgt cggccgcgaa caccggccgt tcgccggttc cgatcaggcc
      121 atcgagcgtc cggcgctggg cgggagtgag cccggcggtc gcctgcatgc ggcggatggt
      181 aggagtagcc gccgacatgg cggagacgcc cgacgacatc ctgcgagccc tcgccgaccc
      241 cgagcggctc acgatcgcgg ggcacctcgc ccgttcggac gcggcctcgg ccgagcttgc
      301 ggacgccctg gacctccccg tgccacggct gcgcaggcac ctgagccggc tcgtcgccgc
      361 cggcgtcgtt cgcctgaagg acgaccggcg gacctatcgg ctcgatgccg agaccctccg
      421 ccgggccacc gagcaggtcg ggccgccgcg ggacgcggga ctcgcgctcg gtgcggcgag
      481 cgacgacgag gaggccgtgc tccgagcgtt cttccgcgac ggccgcctga ccgaggtgcc
      541 cgcgaaggag tcgaagcgac gcatcgtgct cggacggatc gcgctcgagt tcgagcccgg
      601 cgtggactac gacgaggcga ccgtcaacgt gatcgtcgga cgcttctcca ccgaccacgc
      661 cgcgcttcac cgctacctcg tcgacgaggg gttcctcgac cgcgatcacg gccgctactg
      721 gcgcaccggg ggacgcgtcg acctctcgta ggccccaccg agagcccggc ccgacgcgtg
      781 ccgggcggaa gggggcgctc cgctagactc cggaggatca tggccaagac gcaggatctt
      841 ccccagcctt cggtgcgcac ggtcgaggac cgcatcgacg agctccgaaa gcgccgggcg
      901 gccctgctcg acggctccag gaaggacgcc gtccgcaagc agcacgagaa gggcaagctg
      961 acagcacgcg agcgcgtcga gctgctggtg gaccgggatt ccttccagga gaccgacccc
     1021 ttcgccgtcc accgttcgca tgacttcggg atcgaacgca accgccccgc gggcgacggc
     1081 gccatcaccg gctacggcac cgtggatggg cgaagggtct tcgtcgcctc gcaggacttc
     1141 acggtgttcg gaggatcgct cggtgagatc cacgcgcaga agatctgcaa ggtccaggac
     1201 ctggcgatgt cgaccggggc tccgttcatc cagatcaacg actcgggcgg ggcgcggatc
     1261 caggagggcg ccgcgagcct cgccggctac ggctacgtgt tcgagcgcaa cgtgcgcgcc
     1321 tcgggcgtga tcccacagat cagcgtgatc atggggccgt gcgccggggg cgccgtctat
     1381 tcccccgcca tcaccgactt cacgttcatg gtccgcgaga cctcgcacat gttcatcacc
     1441 ggtcccgacg tgatcaaggc cgtgaccggg gaggaggtca cgttcgagga actcggcggc
     1501 gcgatgacgc acgcgagccg atccggggtc gcgtcgttcg tgagccagga cgaggaggag
     1561 tgcctcgcga tggttcggca cctcctgtcc tacctcccct cgaacaacct ggaggacgcg
     1621 cccgcgttcg cgccggtcga cgaccccgac cgccacgacg agggcctgac gcatgtgatc
     1681 cccgattcgg cgcgggagcc gtacgacatg cacgaggtga tccgccgcat cgtggacgac
     1741 ggtgacttct tcgaggtctt cccgttctgg gcgatgaacg tggtgacggg gttcgcgagg
     1801 ctggacggcc gcgcggtcgg cgtcgtggcg aaccagccca aggtgctcgc cgggacgctc
     1861 aacatcgacg cgtccgagaa ggccgcgcgg ttcgttcgga cgtgcgacgc gttcaacatc
     1921 ccgatcgtca cgttcgtcga cgtgccgggg ttcttgccgg gcaccgacca ggagtaccag
     1981 gggatcatcc gtcacggcgc caagctgctg tacgcgttca ccgaggcaac ggtgccgagg
     2041 ctcaccgtca tcacccgcaa ggcctacggg ggcgcgtacc tcgtgatgaa ctcgaagcac
     2101 ctgcgcgccg acgtgtcgtt cgcgtggccg accgccgaga tcgccgtgat gggaccggaa
     2161 ggcgcggtga acgtcgtctt ccgcaagcag atcgagaagg ccgaggaccc ggtcgcggcg
     2221 cgcgccgagc tgatccagca gtaccaggac aagttcgcga cgccgtacat cgcggccgaa
     2281 cgagggttca tcgacgacgt gatcgaaccc gccgagacga ggccgcgcct gatcagggcg
     2341 ctccggatgc tctcgaccaa gcgcgagatc gttccggcgc gcaagcacgg gaacatcccc
     2401 ctatgagcgc agggccgagc ggagcggcga gccggccgtg agcgacgacc gccgcttcga
     2461 gctgcccgac gacctctcgc ccgacgagga gcgcgcgatc atccgggcgc tcgagcgcta
     2521 cttcgcgcag gagagcccgc atccccaccc gtgggtcctc gccggccgga tcgacgcgac
     2581 ggggttcggc gcgttgcagg cccggcacca cgcggatgcc ccatggcggc gcttcgtcga
     2641 cgtgcccttc gcccgccggg gcgtcccgcc gcaggcggga cgcggcgacg cggggtagat
     2701 gacagcagcg aagaccgcgt ccgcggtcgg cggacgcgac gccgcgctcg aggcgttccg
     2761 ggccgagggg tgctcggcgc cgagatcgtg gggcaacgga cccggcgacg cgtacggccc
     2821 gcacgcccac ggcttccaca aggtgctgtt ctgcctcggc ggctcgatca cgttccacct
     2881 cgacgacgg
//
LOCUS       DATBRK010000264        12338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1916961, whole genome shotgun sequence.
ACCESSION   DATBRK010000264 DATBRK010000000
VERSION     DATBRK010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12338
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916961"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VIH70_07935"
     CDS             <1..1064
                     /locus_tag="VIH70_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8116329.1"
                     /translation="TMMRMPVFTWSILATSLLVILAAPVLTAGLIMVFADRQIGTVFF
                     DPGRGGVVLLWQHVFWFFGHPEVYMLILGAWGIVTEIVSVFSGKPIFSYRGVILSFLL
                     ITALSFSVWAHHMFATGAVELPFFSVTTELISIPTGVLFFIWLGTMWKGRLRFEPPML
                     FALGFIAMFLIGGINGVWAASPAMDFAIHDTYWVVAHLHYVLFGGTIFGVMAGMYYWF
                     PKMTGRMLSRKLGIWQFWLQLVGFNLTFFPMHMLGLRGMPRRIADYAPDRGWSFLNLM
                     SSIGAFVIAIAMIVFFVNIFLTLRKPRSAPDDPWESNTLEWATSSPPPPHNFDALPQI
                     RSDRPAYDLRMAAKEQPQT"
     gene            1061..1648
                     /locus_tag="VIH70_07940"
     CDS             1061..1648
                     /locus_tag="VIH70_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8116330.1"
                     /translation="MSAVTVPNERPSREMSGPVMGMVLFVASEAMFFAAFFGGYFTIS
                     GNAKVWPPPGTPDLEIGIPTLLTLILVTSSLVVQLSLRSIRRDDRRRAILLLGVTIAL
                     GIAFLLLQLYDYSQLGFGVKDGTFGTLFYVMTGIHMAHVFGGVVFLSLVFGQAMGGVL
                     SSRNHDSLAAGAIYWDFVDVVWLLLFTTFYLLAPR"
     gene            1653..2027
                     /locus_tag="VIH70_07945"
     CDS             1653..2027
                     /locus_tag="VIH70_07945"
                     /inference="COORDINATES: protein motif:HMM:NF023691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEY8116331.1"
                     /translation="MSAISRISLSLAGFLAIAGTVYGLTSHEAAGTTLFLAGAATFCF
                     VGLVARREGGRPSGPEAGEEAEINVAPTIWPFGFAVAAVIIALGLIVSSWLLIVGAIA
                     FGLSAVGWLRDVGRSRTHADHS"
     gene            complement(2037..2231)
                     /locus_tag="VIH70_07950"
     CDS             complement(2037..2231)
                     /locus_tag="VIH70_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116332.1"
                     /translation="MKGIRDGYDRCVRCGDRVPWGESACRRCNPARLPSPSPTQYHAT
                     VFLVVIVTLVVVTGWLMIRG"
     gene            complement(2309..3142)
                     /locus_tag="VIH70_07955"
     CDS             complement(2309..3142)
                     /locus_tag="VIH70_07955"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY8116333.1"
                     /translation="MTFDPAVVIALVAGVAYVRAERRVRRERGDRRFPRARGRQFLAG
                     LAIVLIALESPIDTASATSFSVHMVQHLLLTMVAAPLLALGAPVTLALLSCSPPNRRR
                     LSAALSRPPLRTLSDPIVAWALFFAVLWGSHLTGLFDASLRSDGVHALEHAAYLATAV
                     LFWMPVVGRDPAPMGLSHPGRILYLFLAMASMAFLGLALSGANHILYPTYEAIEGAAK
                     AAADQRMGGALMWISGMILILPALALVMLDWMRVDEREARRMDARLLRPLAPTSGGPR
                     R"
     gene            complement(3139..4290)
                     /locus_tag="VIH70_07960"
     CDS             complement(3139..4290)
                     /locus_tag="VIH70_07960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8116334.1"
                     /translation="MQPERFEWPNRLRWWDIAWVLFSLANLYAMLLWPDWETVPFHFI
                     WVSLTILYGFRVWRARPTTVILAFVMVLTGALIWIDVSRGFTPLDELTEVPLMAAMFV
                     AMVWHARRRLIAMEETERVSQANLRLLERERRFVQDASHELRTPITVALGHAELIQRI
                     ARDPTLAEDAAVIADELMRLRRLANRLLLLAGAEDPEFLHLEPVDVETIVVDAVRRWT
                     ATRRCWLLGSTEEATVMADADRLEVALDALIENAVNHTREPDTIEVAIHRRGARAVIS
                     VRDTGSGIPAPDLDHIFDRFVRADPGRSRHTGGFGLGLSIVKAIVDAHGGEVHVTSAV
                     GVGTTFEILLPLSPVVADRPEGTWRQGTSGLTPASTPAGRALDAYAREP"
     gene            complement(4280..4927)
                     /locus_tag="VIH70_07965"
     CDS             complement(4280..4927)
                     /locus_tag="VIH70_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8116335.1"
                     /translation="MIDDEKRILSFVRRGLEAEGLAVDCASDGEEGLRRALNHQYDLV
                     VLDLVMPGLDGHEVLRRILARKPSQPILILSAVSDTASKVKSLERGAEDYITKPFSLE
                     ELLARVRARLRIAARSIQTRLSAGSLTLDLIRRQADEGSGPIPLAEREFLLLSELLRH
                     AGHTVSKDRLLASVWGYHFDPRSNVVDVYVRRLRAKLGSGTIVTVRGVGYRIDAA"
     gene            5297..5950
                     /locus_tag="VIH70_07970"
     CDS             5297..5950
                     /locus_tag="VIH70_07970"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8116336.1"
                     /translation="MGPSLPGDHDPVEIRDPRPNIRGRRLAAALLVALVVFIALAIGW
                     AAARGGGSRGALGTAERPAVAVAINEPAPDFELPVIGHKDSTISLSSLKGQVVVLNFW
                     ASWCLPCRDEAPGLRSAWEGSRGQQGVLFLGVNEMDDEGGAQAFQREFHVTYPSVFDP
                     AASLAADYGFFGLPVTYVIDARGVMRYRFAGSVTESDLVDAVRSVLRGVGKETVTNR"
     gene            6188..6712
                     /locus_tag="VIH70_07975"
     CDS             6188..6712
                     /locus_tag="VIH70_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116337.1"
                     /translation="MFSDSRLRRTTIWVAAAAVLSLVSAGCSSGDDAGTTGASGASAA
                     SGGSGAQTGAAGAVDVTLQEYAIIPGAASATAGAVTFDVTNAGPDEVHEFVVIKTDLA
                     PDALPANKDGTVDEEAAGLEPQGEVEEVAVGDTQSLTLDLSAGNYVFICNIYEDDTHE
                     SHYQEGMRVAFTVN"
     gene            complement(6732..7925)
                     /locus_tag="VIH70_07980"
     CDS             complement(6732..7925)
                     /locus_tag="VIH70_07980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8116338.1"
                     /translation="MTTTFTDLGVSGRVIDALGRRGVSDPFAIQSLVLRDAIDGRDVL
                     ARSATGSGKTLAFAIPIVERVRPNDPTPTALVLVPTRELAQQVADDFAEIARATGLRV
                     GVAYGGVSMQQQARGNAKAHVLIATPGRLQDLAERGSVRLDRVRILVLDEADRMLDMG
                     FQPQVDRLVRRIPKDRQTMFFSATLDGAVGNLARAYTNDAVLHEVESAHDTVDGLDHR
                     FVQVESRGKVEALIELLEHDGELTLVFVRTKRGADRLVHKLKQKGLHAEALHGDMPQR
                     ARQRALERFETGKARVLVATDVAARGLDLERISHVVNYDPPQDDKGYVHRVGRTARAG
                     RTGTGITLVTADQQGDVGRMAARLDLHEAFEEEGMKVAPPQVVFSASASGRRSGLRAR
                     RRRVL"
     gene            complement(8132..8782)
                     /locus_tag="VIH70_07985"
     CDS             complement(8132..8782)
                     /locus_tag="VIH70_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY8116339.1"
                     /translation="MVAVIAPPKPRLRGRIHQVAFFVSLPAGVVLILLANGPAAIAVA
                     SVYAVSLAAVFGSSAIYHRGQWTERARRWLKRLDHSMIYVLIAASYTPVAALVLGGPW
                     EVVVLSVIWTGAVVGVTMKMARPDGLSVVSAALYIVLGWLAILALPQLAREMTVAELV
                     LLLAGGLLYTAGAIVFAIRRPDPSPLVFGYHEVWHSFMVAAAACHYAMILLLLLPS"
     gene            8825..10540
                     /gene="ade"
                     /locus_tag="VIH70_07990"
     CDS             8825..10540
                     /gene="ade"
                     /locus_tag="VIH70_07990"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEY8116340.1"
                     /translation="MTSDLVRRIAVARGDEPADLVLSGGRVLSVFTGEFLEADVAIVG
                     QHVAGVGQGYEGTERLDVSGLILLPGFIDGHMHLETTKLRLDQFARAALPWGTTTVVL
                     DPHEIANVFGIDGVRALLRDADEVPLDAYVMVSSCVPASPFESSGATLTPEDIAELLR
                     TEPRAIGLAEMMDYPAVVAGGEDAVAKVAAAGGRHIDGHAPGLSGRALNAYLAAGVRS
                     DHECTTYEEALEKRRLGMWIMIRQGSAARNLEALLPLVLEHGITNCLLCTDDREPHDL
                     QEHGHINDVVREAVALGCPPADAVVMATLNAARYHRLHEHGAVAPGYLADVVAVPDLV
                     SFRPERVWKRGRLVADGGRPTPIASSSLPGWMRESMHVRELEAADFSVVADGAVRVIG
                     VEAGTIVTRALVGEPLRRDGKACADPGRDLAKLAVIERHRDTGRIGVGFAQGFGLQAG
                     ALASSHAHDAHNIVVVGMTDEDMAAAGNHLRAIGGGQVVVMDGRIIAELPCPIGGLLS
                     DLTFEEVAERARVLEEAAGELGTTLPSPFMAMSFLALSVIPELKLTDRGLVDVDRFEL
                     VPLEV"
     gene            complement(10545..>12338)
                     /locus_tag="VIH70_07995"
     CDS             complement(10545..>12338)
                     /locus_tag="VIH70_07995"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY8116341.1"
                     /translation="QLGVDSIRSSTAAGSGHPTSSLSAAHLVAVLWSDHLRYDVADPR
                     SLANDRFVLSKGHASPLIFSVLKAVGAIGDEQLLSFRRFGSPVQGHPAPLPEMPWIDV
                     ATGSLGQGLPIALGMALAMKMDGIPARVWCLMGDSEVAEGSVWEAMENASHHGADNLI
                     AIIDVNRLGQRGPTMLQWDLETYAARARSFGWHPIEVDGHDVGAIDAAFTEAEGSDGP
                     AIVIAKTEKGHGVSFLADKEGWHGKALSQEEADRAIAELGGPRDVSVTPRKPEAGVSP
                     PADVPIVVLPVYAEAIATRKAFGETLAALAVRGDVVAIDGEVANSTHTEDLQKVAPDR
                     FVESYIAEQNMLGMAVGMQVLGKAVFPATFAAFLTRAYDFIRMAAISRANLRLSGSHA
                     GVSIGEDGPSQMALEDLAMMRAVHGSTVLYPCDGNSTARLVATMADLPGISFIRTTRE
                     KTPAIYGDDEDFPVGGSKTLRSSDADRVTLVGAGITVHEALGAARSLEADGIAARVVD
                     CYTIKPIDAETLRAAAFETGLLVTIEDHWIEGGLGDAVLAALAVGGDLSGRVVKVAVT
                     QMPGSGTPEELRDWAGISASAIADRVRSLLR"
BASE COUNT         1924 a         4242 c         4186 g         1986 t
ORIGIN      
        1 tgacgatgat gcggatgccg gtgttcacct ggagcatcct cgcgacctcg ctcctcgtga
       61 tcctcgctgc cccggtgctc acggccgggc tgatcatggt gttcgccgat cggcagatcg
      121 ggaccgtgtt cttcgatccc ggtcgtggag gcgtcgtcct gctctggcag cacgtcttct
      181 ggttcttcgg gcacccggag gtctacatgc tgatcttggg cgcatggggg atcgtgaccg
      241 agatcgtttc cgtcttctcg ggtaagccga tcttcagtta ccgcggcgtg atcctatcct
      301 tcctcctgat caccgccctc tcgttcagcg tctgggcgca ccacatgttc gcgaccggtg
      361 ccgtcgaatt gccgttcttc agtgtgacca ccgagctcat ctcgatcccg accggcgtgc
      421 tcttcttcat atggcttggc acgatgtgga agggcaggct ccgcttcgag cctccgatgc
      481 tgttcgcgct cggcttcatc gctatgtttc tgatcggcgg catcaacggc gtgtgggcag
      541 cctccccggc gatggatttc gcgatccacg acacctactg ggtcgtcgcg catctccact
      601 atgtgctctt cggcgggacg atcttcggcg tgatggccgg gatgtactac tggttcccga
      661 agatgaccgg ccggatgctc tcaaggaagc tcggcatctg gcagttctgg ctgcaactcg
      721 tcggcttcaa cctcacgttc tttccgatgc acatgctggg tctgcgaggc atgcccaggc
      781 ggatcgcgga ctacgcgccg gaccgcgggt ggagcttcct gaacctgatg tcctcgatcg
      841 gcgcgttcgt gatcgcgatc gcgatgatcg tgttcttcgt gaacatcttc ctcacgctgc
      901 gaaagcctcg gtcggcgccg gacgacccct gggagtccaa tacgctggaa tgggccacgt
      961 cctccccacc gcctccacac aacttcgacg ccttgccgca gatccgctcg gatcgcccgg
     1021 catacgacct acgcatggcc gcgaaggagc agccgcagac atgagtgcgg tcacggtgcc
     1081 gaacgagcgt ccctcgcggg agatgagcgg gccggtcatg gggatggtcc tgttcgtcgc
     1141 gtcggaggcg atgttcttcg cggcattctt cggcggctac ttcacgatca gcggcaacgc
     1201 gaaagtgtgg ccgcctcctg ggacccccga tctggagatc ggcatcccaa ccctcctcac
     1261 gctgatcctc gtcacgagca gcctggtcgt gcaattgtcg ctgcgatcga tccgccgcga
     1321 cgatcggcgt cgagccatcc tcctcctggg cgtcacgatc gcgctcggga tcgccttcct
     1381 cctgctccag ctctacgact actcccagct cgggttcggg gtgaaggacg ggacgttcgg
     1441 cacgctcttc tacgtcatga ccggcatcca catggcacac gtcttcggcg gcgtcgtctt
     1501 cctctcgctc gtcttcggcc aggcgatggg cggcgtgctg tcgtcaagga atcacgactc
     1561 cctcgccgcc ggcgccatct actgggactt cgtcgacgtc gtgtggctcc tgttgttcac
     1621 gacgttctac ctcctggcgc ccaggtgatg gcatgagtgc catctcgcgg atctccttat
     1681 cgctcgcagg cttcctggcg atcgcgggca ccgtctacgg gctgaccagc catgaagcgg
     1741 ccggcacgac gctgttcctt gcgggcgcgg cgacgttctg tttcgtgggg ctggtggcgc
     1801 ggagggaggg aggccgaccc tcgggcccgg aggccggcga agaggccgag atcaacgtgg
     1861 cgcctacgat ttggcccttc gggttcgccg tcgccgcagt gatcatcgcg ctcggactca
     1921 ttgtgagttc ttggctcttg atcgtcgggg cgatcgcgtt cggcctctcg gccgtgggct
     1981 ggctccgcga cgtcggacgc tcccggacgc acgccgacca ctcgtagtcg acccgattag
     2041 ccccggatca tcaaccagcc cgtgaccacg acgagcgtca cgatcacgac caggaacacg
     2101 gtcgcgtggt actgcgtggg gctcgggctg ggcagccggg cggggttgca gcgccggcat
     2161 gcggactcgc cccacggaac gcggtcgccg catcggacgc accggtcgta gccgtcccgg
     2221 atacccttca cagttcctgg cctcctcccg ggcctcatgc gtggatggtg ggcccggctc
     2281 gtgcttgatc ctcgggcgtc ggagtcgctc atctcctcgg cccgcccgac gtcggtgcga
     2341 gcgggcgcag caggcgcgca tccatccgcc gcgcctcgcg ctcgtcgacg cgcatccagt
     2401 ccagcatgac cagggccagg gccggcagga tgaggatcat cccactgatc cacatgagcg
     2461 ctccccccat cctctggtcc gcagcggctt tcgccgcgcc ttcgatggcc tcgtaggtgg
     2521 ggtacaagat gtggttcgcg ccggaaagcg caaggccgag gaatgccatc gacgccatcg
     2581 ccaagaagag gtacaggata cggccggggt gggagagacc catgggagcg gggtcgcggc
     2641 caacgacggg catccagaac aggaccgccg tcgccaggta ggcagcgtgc tcgagcgcgt
     2701 ggacgccgtc acttcgcagc gacgcgtcga agagccccgt gagatgggat ccccagagga
     2761 cggcgaagaa cagggcccac gcgacgatcg gatcggagag ggtccggagc ggtgggcgcg
     2821 agagcgctgc ggagagcctc cgccggttgg ggggcgagca tgacaggagc gcgagcgtta
     2881 ccggggcgcc gagcgccaag agaggggctg ccaccatggt gagcagcagg tgctggacca
     2941 tgtgaaccga gaacgacgtc gctgaagccg tgtcgatcgg cgactcgagt gcgatgagga
     3001 cgatcgccaa gcccgcgagg aactgccgtc cgcgagcgcg cgggaaacgg cgatcacccc
     3061 gctcccgacg aactcttcgc tccgcgcgaa cgtacgcgac gccggccacc agggcgatga
     3121 cgaccgccgg gtcaaacgtc atggttcgcg agcatatgcg tcgagtgcgc ggcccgccgg
     3181 ggtcgaggcg ggagtcagtc ccgacgttcc ctgcctccaa gtgccttctg ggcggtcggc
     3241 taccaccgga gacagcggca acaggatctc gaacgtagtt ccgacgccga ccgcgctggt
     3301 gacatgcacc tcgccgccat gggcatcgac gatcgctttc acgatcgaca atccgaggcc
     3361 gaagccgccg gtatgccggc tccgccccgg atcggctctc acgaaccggt cgaagatgtg
     3421 gtccaggtcg ggggccggga tccccgaccc cgtgtcgcgg accgagatca cggcccgagc
     3481 accgcggcgg tggatcgcca cctcgatcgt gtctggctcc cgcgtgtggt tcaccgcgtt
     3541 ctcgatcagg gcgtcgagcg cgacttcgag gcggtcggcg tcggccatca ccgtcgcctc
     3601 ctcggtgctt ccgagaagcc aacaacgacg cgtcgcggtc cagcggcgga ctgcgtcgac
     3661 cacgatcgtc tccacgtcga ccggctcgag gtggaggaac tcggggtcct ctgctccggc
     3721 gaggaggaga aggcggttgg cgagcctacg caggcgcatc agctcgtcgg cgatgacggc
     3781 ggcatcctcg gcgagggtag ggtctctcgc gatccgttgg atcagctccg catgccccag
     3841 ggcgacggtg atcggcgtcc gcagctcgtg cgacgcgtct tgcacgaacc gccgctctcg
     3901 ttcgagcaga cgcaggttcg cctgcgacac acgctcggtc tcctccatgg ctatcaggcg
     3961 ccgacgcgca tgccacacca tcgccacgaa catagctgcc atgaggggga cctcggtgag
     4021 ctcatcgagc ggcgtgaagc ccctcgacac gtcgatccag atcagtgcgc ccgtcaacac
     4081 catcacaaag gcgaggatca ccgtcgtggg acgggccctc cacacgcgga acccgtagag
     4141 gatcgtcagg ctcacccaga tgaagtggaa cggcaccgtt tcccagtcag gccacaagag
     4201 catcgcgtac aggttcgcga gcgagaacag cacccatgcg atgtcccacc accgcaagcg
     4261 gttcggccat tcgaatctct caggctgcat cgatccggta ccccacacct cgaacggtga
     4321 cgatcgtccc ggagccgagt ttcgcgcgaa ggcgccgcac atagacgtcg acgacgttcg
     4381 agcggggatc gaagtggtaa ccccagaccg acgcgagcag gcgatccttc gacaccgtgt
     4441 ggccggcgtg gcgcagcaac tcgctgagga gcaggaactc gcgctcggcg agcgggatcg
     4501 gaccagatcc ctcgtcggcc tgccgccgga tcaggtcgag ggtcagtgag cccgccgaca
     4561 ggcgcgtctg gatcgaacgc gccgcgatcc gaagccgcgc ccgcacgcgt gccaggagtt
     4621 cctcgagcga gaacggcttc gtgatgtagt cctcagcgcc tcgctcgagg ctcttcacct
     4681 tcgatgcggt gtcgctcacc gcggacagga tcaggattgg ctgcgacggc ttgcgcgcca
     4741 ggatgcgccg gagcacctca tggccgtcaa gccccggcat cacgaggtcc aagacgacga
     4801 ggtcgtactg atgattgagc gcgcggcgca ggccttcctc accatcgctc gcgcaatcga
     4861 ccgccaagcc ctcggcctct aaaccgcgac gaacgaagct gaggatccgc ttctcgtcgt
     4921 cgatcacgag gatgcgaccg atcggaacta ggtcatcccc tgaccgctca ggcgaagtca
     4981 tgcccccacc tcgcctgcca cattagcgac taccgttcgc gctggaaagt gggggagggc
     5041 ggcttgcctg cgccggtagc tcgggcgacc cgacgatcag gaaccctggc ccggagcatc
     5101 ggccggtcgg ccggagctcc ggagagcccg atccttcggc cacctcggga gatcctcggg
     5161 tccggacagg gatgaaccgg aacgacctcc ctacgatcct ctcgtggtcc caaccacatc
     5221 cggacctgtg tctgctgctt acctagcgcc atggaggcgg gatcaagacg actgctcctc
     5281 gttgtagatt gccttcgtgg gaccttcgct ccccggcgat cacgatcctg tcgagatccg
     5341 cgacccaagg cccaacatcc gcggccggcg attggcagcg gctctgttgg tggcgcttgt
     5401 tgtcttcatc gccctcgcga tcgggtgggc ggccgctcgg ggtggcggtt cccgtggtgc
     5461 cctcggcacg gccgagcggc cggcggtggc agtcgccatc aacgagcccg ctccagactt
     5521 cgagctcccg gtcataggtc acaaggactc caccatctcg ctctcctccc tcaaggggca
     5581 ggtcgtcgtc ctcaacttct gggcgagctg gtgccttccg tgtcgggacg aggcgcccgg
     5641 ccttcgatcg gcatgggagg ggtcccgggg ccaacaaggc gtcctgttcc tgggggtgaa
     5701 cgagatggat gacgaagggg gcgcgcaggc gtttcaacgg gagtttcacg tgacctatcc
     5761 aagcgtgttc gatccggcgg cgtcgctggc ggccgattac ggtttctttg ggctgcccgt
     5821 gacctacgtg atcgacgcac gaggggtgat gcgataccgg ttcgccggat ccgtgaccga
     5881 gtccgacctg gtcgatgcgg tgcgctcggt tctccgagga gtcggcaaag aaaccgtgac
     5941 caaccgttga cgggtggctc ttcgccgtgc gactgggggg tcatgacggc ggcgtggtcg
     6001 gcgacgatca aacgggcgat gcgaaaggcg ctacgtgctc atctgcaacg tgatcctccg
     6061 actcctgctc cccgacgagc acgggtgcta gggtcgtttg cccccagtac cagcgggccg
     6121 aaggctctgt cgccggcccg aagccgggtc gacactcgaa ccttcgaccc gaaaggacgg
     6181 gggcatgatg ttctcggatt ctcggctcag gagaacgacg atctgggtcg ccgccgccgc
     6241 cgtgctgagc ctggtgtcag cggggtgcag ctcgggcgac gacgcaggaa cgaccggcgc
     6301 ctccggagcg tcggccgcgt ctggagggtc gggcgcgcag acgggcgcgg ccggggccgt
     6361 cgacgtgacg ctccaggagt acgcgatcat ccccggcgct gcctcggcaa ccgcgggtgc
     6421 cgtgacgttc gacgtcacca acgccgggcc ggatgaagtc cacgagttcg tcgtcatcaa
     6481 gaccgatctc gcgcccgatg cgctgccagc gaacaaggac gggacggtcg atgaagaagc
     6541 cgccggtctc gaaccgcagg gcgaggtcga ggaggtcgcg gtgggcgaca cgcagagctt
     6601 gacgctcgac ctttcggcag ggaactacgt gttcatctgc aatatctacg aagacgacac
     6661 gcacgagtcc cactaccagg aagggatgag ggtcgccttc acggtcaact agcgcgacgg
     6721 tttggttcgg gctagaggac cctgcgtcgg cgtgcccgca ggcccgagcg cctgccgctg
     6781 gcgctcgcgg agaagaccac ctgcgggggc gccaccttca tgccctcctc ctcgaacgcc
     6841 tcgtgcaggt ctaggcgcgc ggccatccgg ccgacgtcgc cctgctgatc cgccgtcacc
     6901 agggtgatgc ccgtcccggt ccgtccggcc cgggccgtgc gtccgacgcg gtggacatag
     6961 cccttgtcgt cctggggcgg gtcgtagttc acgacgtggc tgatccgctc cagatccaga
     7021 ccgcgggcgg cgacgtccgt cgcgaccagg accctggcct tgcccgtctc gaacctctcg
     7081 agcgcacgct gacgcgcccg ctgcggcatg tcgccgtgga gcgcctcggc gtgcaacccc
     7141 ttctgcttca gcttgtgcac cagacggtcg gcgcctcgct tggtcctcac gaagacgagc
     7201 gtcagctccc cgtcgtgctc gaggagctcg atcagcgcct cgaccttgcc gcgcgactcg
     7261 acctggacga agcgatggtc gagcccgtcg acggtgtcgt gcgcggattc gacctcgtgc
     7321 aggacggcgt cgttcgtgta ggcgcgcgcc aggtttccga ccgcgccgtc caacgtggcg
     7381 gagaagaaca tcgtctgccg gtccttgggg atgcgccgga ccaggcggtc gacctggggc
     7441 tggaaaccca tgtcgagcat ccggtcggcc tcgtcgagga cgaggatccg cacgcggtcg
     7501 aggcggacgc tgccgcgctc cgcgagatcc tgcaggcgtc ccggggtggc gatcaggacg
     7561 tgggccttgg cgttgccgcg ggcctgctgc tgcatgctga ccccgccgta ggcgacgccg
     7621 acgcgcaggc ccgtggccct cgcaatctcg gcgaaatcgt cggccacctg ctgcgcgagc
     7681 tcgcgcgtcg gcaccaggac gagcgcggtc ggcgtggggt cgttcggtcg gactcgctcg
     7741 acgatcggaa tcgcgaacgc gagcgtcttg ccggagccgg tcgccgaacg ggcgagcacg
     7801 tctcgtccgt cgatcgcgtc tcggagcacg agcgactgga tcgcgaacgg atcggatacc
     7861 cctcgcctac cgagggcgtc gatcacgcgc ccggacacgc ccaggtcggt gaacgtggtg
     7921 gtcatgttct ctccttcttc cggcgcgtcg agcgcgccgc atctcggccg gcgacgcctg
     7981 cgtgggtgaa ccgagcgggc gcggccaggt ggatcgtggg gaggtttggc cttgccccga
     8041 gaccccggcc agatgatccg gaaggaggaa cgaggagcga aacctcacgg acggcgttgc
     8101 cgtcctcgac gaccacgata ccagctaccg gtcaggaggg aagcaggagc agcaggatca
     8161 tcgcgtagtg gcacgccgcc gcggccacca tgaacgagtg ccacacctcg tggtagccga
     8221 agaccaacgg cgagggatcg ggccgccgga tcgcgaacac gatcgcgccg gccgtgtaga
     8281 gcaggccccc ggccagcagg aggacgagct ccgcgaccgt catctcgcgc gcgagctgcg
     8341 ggagggccag gatggccagc cagccgagca cgatgtagag cgcggcgctc accacggaga
     8401 gcccgtcggg gcgagccatc ttcatggtca ccccgaccac cgctcccgtc cagatcacgg
     8461 acaggaccac gacctcccaa ggccccccaa gcacgagcgc cgcgaccggc gtgtagctcg
     8521 ccgcgatcag cacgtagatc atcgagtggt cgagtcgctt caaccaccgc ctcgcgcgtt
     8581 cggtccactg gccccggtgg tagatggcgc tcgagccgaa cacggccgcg aggctcaccg
     8641 cgtacacgct ggcaacggcg atcgcggcgg gtccgttcgc gagcaggatc agcaccacgc
     8701 ctgcgggcag cgacacgaag aacgcgacct ggtggatccg gcctcgcaaa cggggcttgg
     8761 gcggtgcgat gacggcgacc aagacccctc atcctacccg ggtggcatgg gctaccctgc
     8821 gtcgatgacg agcgatctcg tccgccggat cgccgtcgcc cgcggcgacg agcccgccga
     8881 cctggtgctt tccggggggc gggtgttgtc cgtgttcacg ggcgagttcc tcgaggccga
     8941 cgtcgcgatc gtcggccaac acgttgccgg cgtcggtcag ggctacgagg ggaccgaacg
     9001 cctcgacgtc tcgggcctga tcctgctgcc cggcttcatc gacgggcata tgcacctgga
     9061 gacgaccaag ctccggctcg accagttcgc gcgggcggcg ctcccgtggg ggaccaccac
     9121 cgtcgtcctc gacccgcacg agatcgccaa cgtgttcggg atcgatggcg tccgcgcgct
     9181 cctgcgcgac gccgacgagg tcccgctcga cgcctacgtg atggtctcct cgtgcgtgcc
     9241 ggcctcgccg ttcgagtcct cgggagccac gctcacaccc gaggacatcg ccgagctgct
     9301 gcggaccgag ccgcgggcga tcgggcttgc cgagatgatg gactacccag cggtcgtcgc
     9361 gggaggcgag gacgcggtcg ccaaggtcgc cgccgccggg gggcgtcaca tcgacggtca
     9421 cgcccccggg ctctcgggcc gcgcattgaa cgcgtacctc gcggcggggg ttcggagcga
     9481 ccacgagtgc acgacgtacg aggaggcgct cgagaagcgc cgcctgggga tgtggatcat
     9541 gatccgccag ggctcggcgg cgcggaacct ggaggcgctc ctcccgctcg tgctcgagca
     9601 cgggatcacg aactgcctgc tctgcacgga cgatcgagag ccacacgacc ttcaggagca
     9661 cggacacatc aacgacgtgg tccgcgaggc ggtggcgctc ggctgcccgc ccgcggacgc
     9721 cgtcgtgatg gcgacgctca acgctgcccg gtaccaccgc ctccacgagc acggcgcggt
     9781 cgcgcccggc tacctcgccg acgtcgtggc ggtgccggac ctcgtgtcgt tccggcccga
     9841 gcgggtctgg aagcgcggtc ggctggtcgc cgacggcggg cggcccacgc cgatcgcgtc
     9901 gtcgtcgttg cccggttgga tgcgggagag catgcacgtg cgcgagctcg aggcggccga
     9961 cttctcggtc gtcgccgacg gggccgtccg cgtgatcggc gtcgaagcgg gcacgatcgt
    10021 cacccgagcc ctggtcggcg aaccgctgcg ccgagacggc aaggcgtgcg ccgacccggg
    10081 gcgcgacctg gcgaagctcg ccgtgatcga gcggcaccgg gacacggggc gaatcggcgt
    10141 tggcttcgcg caggggttcg ggctgcaggc gggggcgctg gcatcgagcc acgcgcacga
    10201 cgcccacaac atcgtggtgg tgggcatgac cgacgaggac atggcggcgg cggggaacca
    10261 cctccgtgcc atcggcggcg gccaggtcgt cgtgatggac ggcaggatca tcgccgagct
    10321 gccctgcccg atcggcggcc tcctgtcgga cctgacgttc gaagaggtcg cggaacgcgc
    10381 ccgcgtcctg gaggaggcgg ccggggagct cggcaccacg ctcccgtcgc cgttcatggc
    10441 gatgtcgttc ctcgccctgt ccgtgatccc cgaactgaag ctcacggacc gcggtctcgt
    10501 cgacgtggac cggttcgagc tcgttccgct cgaggtctga ggactcagcg cagcagcgaa
    10561 cggaccctgt cggcgatcgc ggacgccgag atcccggccc aatcgcggag ctcctcgggg
    10621 gtccccgagc cgggcatctg cgtcacggcg accttgacga cccggcccga gaggtctccg
    10681 ccgaccgcga gcgccgcgag gaccgcgtcg ccgagccctc cctcgatcca gtggtcttcg
    10741 atcgtgacga gcaggccggt ctcgaacgcc gccgcgcgga gcgtctccgc gtcgatcggc
    10801 ttgatcgtgt agcaatccac gacccgggcc gcgatgccat cggcctccag cgaccgcgcg
    10861 gccccgagcg cctcgtggac cgtgatcccc gcgccgacga gcgtgacccg gtccgcgtcc
    10921 gaggaccgca gggtcttcga accgccgacc gggaagtcct cgtcgtcccc gtagatcgcg
    10981 ggggtcttct cccgggtcgt ccggatgaaa gagatccccg gcaggtcggc catcgtcgcc
    11041 accaggcgcg cggtcgagtt gccgtcgcac gggtagagca cggtcgagcc gtggacggcg
    11101 cgcatcatcg ccaggtcctc gagcgccatc tgcgacggac cgtcctcgcc gatcgacacc
    11161 cccgcatggg agccgctcag cctgaggttc gctcggctga tcgcggccat ccggatgaag
    11221 tcgtacgccc tggtcaggaa cgcggcgaac gtcgccggga agaccgcctt tcccagcacc
    11281 tgcatgccga ccgccatccc cagcatgttc tgctcggcga tgtagctctc cacgaatcgg
    11341 tcgggcgcga ccttctggag atcctcggtg tgggtcgagt tcgcgacctc accgtcgatc
    11401 gcgaccacgt ccccccgcac cgcgagcgcc gcgagcgtct ccccgaacgc cttccgtgtc
    11461 gcgatcgcct ccgcgtacac cggcagcacc acgatcggta cgtcggccgg cggcgagacc
    11521 ccggcctccg gcttgcgtgg cgtgaccgag acgtcgcgcg gcccgccgag ctcggcgatc
    11581 gcgcgatccg cttcctcctg ggacagcgcc ttgccgtgcc atccctcctt gtcggccagg
    11641 aacgagacgc cgtggccctt ctcggtcttg gcgatcacga tcgccggccc gtccgaaccc
    11701 tccgcctcgg tgaacgcggc gtcgatcgcg ccgacatcgt gaccgtcgac ctcgatcgga
    11761 tgccagccga acgaacgcgc gcgggcggcg tacgtctcca ggtcccactg gagcatcgta
    11821 gggccgcgct ggcccagccg gttcacgtcg atgatggcga tcaggttgtc ggcaccatgg
    11881 tgcgaggcgt tctccatcgc ctcccacacc gagccctcgg cgacctcgga gtcccccatc
    11941 aggcaccaga cgcgcgcggg gatgccgtcc atcttcatcg cgagcgccat cccgagcgcg
    12001 atcggcagac cctggcccag cgacccggtc gcgacgtcga tccagggcat ctcgggcagc
    12061 ggggcggggt gcccctgcac cggcgacccg aaccgtcgga acgacagcag ctgctcgtcg
    12121 ccgatcgccc cgacggcctt caggacggag aagatcagag gcgacgcgtg gcccttcgac
    12181 aggacgaagc ggtcgttcgc caggctcctc gggtcggcga cgtcgtagcg gaggtggtcg
    12241 gaccagagca cggcgaccag gtgggccgcc gagagcgagg acgtgggatg acccgacccc
    12301 gccgccgtcg agctcctgat cgagtccacg ccgagctg
//
LOCUS       DATBRK010000265         7292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_479204, whole genome shotgun sequence.
ACCESSION   DATBRK010000265 DATBRK010000000
VERSION     DATBRK010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479204"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VIH70_08000"
     CDS             <1..792
                     /locus_tag="VIH70_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8116342.1"
                     /translation="DLAWAPDEIFALANASELPTFTGPVIRAAGPMLTAAGGYPTSAS
                     WAPPGTGRELTGPEDAAAAVAELADHGAAAIKVSLNSEAGPTPSDAELAAIVRAAHER
                     ELPVTAHVQGRGQAERALGARMDEFAHTPWTERLSDLLLASAARSMRIVSTLDILSFG
                     DVTPDLRIACDNLVRFRAAGGTVVYGTDLGNGSIPAGIHLREALLLHEAVRMTPEEVL
                     SAMTAGPLAVDGPADMIVLGSDPLLRLEALGDLRVVVRAGRVVSG"
     gene            complement(798..1382)
                     /locus_tag="VIH70_08005"
     CDS             complement(798..1382)
                     /locus_tag="VIH70_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116343.1"
                     /translation="MDTHTHRRRIALLLLLAAAATTLGGQRPAPAREPLVIHGANAAE
                     ERAIDWSIRRYREAGLEGMPDLEVYVHRSNGGCRDGIGYYLAGRIDLCTTESSEPYQR
                     KFALHEMAHGWIETNVGGAVLDRFMEVRGIAAWNDRSSDWKQRGTEQAAEIVTWGLGE
                     GEIAPLLPEALDAPALGRLYELLTGREPITPAAR"
     gene            1515..5009
                     /locus_tag="VIH70_08010"
     CDS             1515..5009
                     /locus_tag="VIH70_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY8116344.1"
                     /translation="MEFLILGPLEVSEDGRKLALGGPKQRAVLAHLILRANRVVPSDL
                     LIDGLWGEEPPESARNTLQTYVYRLRKVLGDGRIEGRDGAYVLAALPDEIDAARFEAL
                     LKQGKASIATNPAGAAATLSDALSLWRADALADVSDEPSLRGEAARLEELRLAATEHR
                     IAAEIAMGGHSTVVAELESLTARYPLRERMWANLMLALYRFGRQAEALHTYERAREVL
                     AEELGTEPSAELQYLHEQILRRDPSLGRTEPTRTTVRPSRVDLQPGTEVAGYRIARTL
                     GRGGMSVVYLAEHEWLQRKVALKVLAPQLAEDERFRERFVRESRLAASLDHPNVIPIY
                     EAGASGGDLFIAMRYVEGTDLRTLLHEGGALEPARALDIVRQVAAALDAAHEQGLVHR
                     DVKPANVLLARKRGSEAGEHVYLSDFGLTKRSASDSGITGTGQFVGTLDYAAPEQFKG
                     GTPDARTDVYSLGCVLFECLTGGPPFTSENDAGLMYAHLQEPPPSVTAVHPGLSGAID
                     RVLARAMAKAPSDRIGSAGALADEASQALGVGVDGAAGSTRERTTLRRVIPALFAAVA
                     LVAGIGLASVFGGASPPAADGPPSSPSVVASPTPEPTFRTIERPLSGDESRLLGYIPE
                     EIAQDCLPLDRDEPIQGELAALACRTKDVEVLYELFPTRELMDQAFQSGANSHRAPDG
                     ECASDRVAVTPYTIGGAPTGRLLCYTIEHGPSAFGALQVRPDESHLEWTDENAFIYAH
                     AVRKDLGDLSLYEWWVTSAGPLVPGTDGAAVTEKDRSAAAVPPPPDGSYLVLPRGGCA
                     DFEDATCSMRIDAGTYELWFTGDATPSEAGQILMEKPNAVVFAPRIGYCFQGRESGSR
                     RSTKPAGYTWSARGDTLSFERTSGGHCAGPQKLTHEPWTRAPEGVIAIETGGDIGLMD
                     AGGVIEQKFFPDTRPNGFPDWSPDGSMIAFSGGSETGADVYVMDGDGTDVTLLTGTPG
                     DEIQPAWSPDGSRIAFAFDDLGDPDFRSGIAIMNPDGSARTDLLIRENEPVGSPLWSP
                     DGTRIAFTAFSQDGLRPADAYVMDADGSEVKKLGGGPTLALAWTPDGRHLLVSREGSL
                     LIVEPDGTHERVVLADPPGGGVLEADWSPDGRWIVLSAPEASGHGQFDAMYLMRVGGG
                     DVFFLGSGFGPDWRPEAP"
     gene            5006..6111
                     /locus_tag="VIH70_08015"
                     /pseudo
     CDS             5006..6111
                     /locus_tag="VIH70_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
     gene            complement(6124..>7292)
                     /locus_tag="VIH70_08020"
     CDS             complement(6124..>7292)
                     /locus_tag="VIH70_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8116345.1"
                     /translation="DPRRTPTTDRDLGSYRSEFPVLERKAYLISASLGPASDRSRRYL
                     DGYIDAWATKGAPDHVWFEDIFPRMRGLKTSFARLAGCDADELAITTNISIAMSTFAS
                     ALDFGERTKVVMSELDFPTDGHVWLAHARRGVEIEWLRSPDGLTIPLEEYDRAIDERT
                     AVVMVNRVLYRSSAIVDAKEVCRIARERGALSFLDDYHGIGIVPLDLHDVGCDAYAAG
                     VLKWLCGGPGLAFLYVRRGLIASLEPMVTGWFGTAEPFAFDTRHLEYHPTARRFEHGT
                     PPAPVFFIAQGGLDVIAEVTPERIRARQGVLGDHVIARADKLGLSVRTPRDRASRGGV
                     VNVGVGPEARKICHALLERDVCTDYRGDGLRISPHFFNTEDDIDRCFDELRPLL"
BASE COUNT         1047 a         2535 c         2632 g         1078 t
ORIGIN      
        1 gatctcgcgt gggcgccgga cgagatcttc gcgctcgcga acgcctccga gctcccgacg
       61 ttcaccggac cggtgatccg agccgcgggt cccatgctca ccgccgcggg cgggtacccg
      121 acgtcggcct cctgggctcc gccggggacc gggcgcgagc tgacgggtcc cgaggacgcg
      181 gccgccgcgg tcgccgagct cgccgatcac ggtgcggcgg cgatcaaggt ctccctgaac
      241 tcggaggccg gcccgacgcc ctcggatgcc gagcttgccg cgatcgtcag ggcggcccac
      301 gagcgcgagc tccccgtcac cgcccacgtc caggggcgcg gccaggcgga gcgggccctc
      361 ggggcgagga tggacgaatt cgcgcacacg ccgtggacgg agcggctctc ggacctcctg
      421 ctcgcgtcgg ccgcacggtc gatgcggatc gtctcgaccc tggacatcct ctcgttcggc
      481 gacgtgacgc ccgacctgcg gatcgcgtgc gacaacctcg tccgcttccg ggcggccggt
      541 ggcaccgtcg tgtacggcac cgatctcgga aacgggtcga tccccgccgg gatccatctc
      601 cgcgaggcgc tccttctcca cgaggccgtc cggatgacgc ccgaggaggt cctttcggcg
      661 atgacggcgg ggccgctcgc ggtggacggg ccggccgaca tgatcgtgct cggctccgac
      721 ccgctcctcc gactcgaggc gctcggcgat cttcgagtgg tggtgcgcgc cgggcgcgtc
      781 gtgtctgggt agcgccgtca gcgcgcggcc ggggtgattg gctcgcggcc ggtgagcagc
      841 tcgtagagcc gaccgagcgc gggtgcatcg agggcttcgg ggagcagcgg cgcgatctcg
      901 ccctcgccca gtccccaggt gacgatctcg gccgcctgct ccgtgccccg ctgcttccag
      961 tcggagctcc gatcgttcca ggcggcgatc ccgcggacct ccatgaaacg gtccaggacc
     1021 gcgccgccga cgttggtctc gatccatccg tgcgccatct cgtggagcgc gaacttgcgc
     1081 tggtacggct cgctcgactc cgtggtgcac aggtcgatcc gtcccgcgag gtagtagccg
     1141 atcccgtccc tgcagccccc gttcgaccgg tgcacgtaca cctcgaggtc gggcatccct
     1201 tccaagcccg cctcgcggta acggcggatc gaccagtcga tcgcgcgctc ctcggccgcg
     1261 ttggccccgt ggatcaccag gggctcgcgg gcgggggcgg gccgctggcc gccgagggtg
     1321 gtcgcggccg cggcgagcag gagcaggaga gcgatccggc ggcggtgggt gtgggtgtcc
     1381 atgaccgcac cctcgtccgg tgccttcgca gcctggccgc aacccggtcg caacccgctg
     1441 gaacgtgatg tgcatcaccg gtgcccctgt gcgtggcttc cgttgtcggg gatgccgtcg
     1501 tacggttcgc ggcgatggag ttcctgatcc tgggcccgct cgaggtctcc gaggacgggc
     1561 ggaagctcgc cctcggcggc ccgaagcagc gggcggtcct cgcgcacctg atcctgcgtg
     1621 cgaatcgggt cgtcccgtcg gatctgctga tcgacggcct ctggggggag gaaccgcccg
     1681 agagcgcccg caacacgctg cagacgtacg tctaccgcct gcgcaaggtc ctcggcgacg
     1741 gtcggatcga ggggcgcgac ggcgcgtacg tgctcgcggc cctacccgac gagatcgatg
     1801 ccgctcggtt cgaggcgctg ctgaagcagg ggaaggcctc gatcgcgacg aaccccgccg
     1861 gcgccgccgc gacactatcc gacgcgctct cgctgtggcg ggcggacgcg ctcgccgacg
     1921 tgtccgacga accatccctg cggggtgagg ccgcgaggtt ggaggagctc cgactcgcgg
     1981 ccaccgagca ccggatcgcc gccgagatcg cgatgggagg ccactcgacg gttgtggccg
     2041 agctcgagtc gctcacggcg cgctacccgc tccgcgagcg gatgtgggcg aacctgatgc
     2101 tcgcgctcta ccggttcggg cgccaggccg aggcgctcca cacctatgag cgggcgcgcg
     2161 aggtcctggc cgaggagctc ggcaccgaac cctcggccga gctgcagtat ctgcacgagc
     2221 agatcctgcg gcgagatccc tcactcggcc ggaccgagcc gacccgaacg accgtccggc
     2281 cgagccgcgt ggacctgcag cccggcaccg aggtcgccgg ctaccggatc gcgcggacgc
     2341 tcggtcgcgg aggcatgagc gtcgtctacc tcgccgaaca cgaatggctc cagcgcaagg
     2401 tggccctcaa ggtgctcgca ccgcaactcg ccgaggacga acgcttccgg gagcggttcg
     2461 tgcgtgagtc gcggctcgcc gcgtcgctcg accacccgaa cgtgatcccg atctacgagg
     2521 cgggagcctc cggtggcgac ctgttcatcg cgatgcgcta cgtggaggga acggacctgc
     2581 gcacgctgct ccacgaaggc ggtgcgctcg agcccgccag agctctcgat atcgttcggc
     2641 aggtggccgc ggcgctcgac gccgcacacg agcaggggct cgtgcatcgg gacgtcaaac
     2701 ccgccaacgt gctcctcgcg cggaagcggg gctcggaggc gggggagcac gtctacctct
     2761 cggatttcgg actgacgaag cggtcggcca gcgactccgg catcaccggg accgggcagt
     2821 tcgtcgggac gctcgactac gcggcgcccg aacagttcaa aggcggcacc ccggatgccc
     2881 gcaccgacgt gtactcgctc gggtgcgtgc tgttcgagtg cctcacgggc ggcccgccgt
     2941 tcacgtcgga gaacgacgcg ggtctgatgt acgcgcacct ccaggagccg ccgccctcgg
     3001 tcaccgccgt tcatcccggg ctgtcgggtg cgatcgacag ggtcctcgca agggcgatgg
     3061 cgaaggcgcc gtccgaccgg atcggctccg ccggagccct cgcggacgaa gcatcccaag
     3121 cgctcggcgt cggcgtggac ggggcggccg gctccacgcg cgaacggacc accctgcgcc
     3181 gggtcatccc ggcgctcttc gccgcggtgg ccctcgtcgc ggggatcggg ttggcatccg
     3241 tcttcggcgg ggcgtcgcca ccggcggcgg atggcccccc atcgtcaccg agcgtggtcg
     3301 cgagccccac gccggagccc accttccgga cgatcgagcg gcccctcagt ggggacgagt
     3361 cccggctcct cggctacatc ccggaggaga tcgcgcagga ctgccttccg ctggaccgcg
     3421 acgagccgat ccagggcgag ctggcagcgc tcgcgtgccg cacgaaggac gtggaggtgc
     3481 tctacgagct cttcccgacc cgagagctca tggaccaagc cttccaaagc ggcgccaaca
     3541 gccaccgggc accggacggc gaatgcgcgt cggaccgggt cgccgtcacg ccgtacacga
     3601 tcgggggtgc cccgaccggg cgcctgctct gctacacgat cgagcacggg ccatcagctt
     3661 tcggtgccct gcaggtgcga ccggacgagt cgcacctcga gtggacggac gagaacgcct
     3721 tcatctacgc gcacgcggtc aggaaggacc tcggcgacct cagcctgtac gagtggtggg
     3781 tgacctcggc gggcccgctc gtcccgggga cggacggcgc ggcagtgacc gagaaggatc
     3841 gctcggcggc ggccgtcccg cctccacctg acgggtcgta cctcgtgctc ccgcgcgggg
     3901 ggtgtgcgga tttcgaggac gccacgtgtt cgatgcggat cgatgcgggc acctatgagc
     3961 tgtggttcac gggcgatgcc actccatccg aggcgggcca gatcctgatg gagaagccga
     4021 acgccgtcgt gttcgctcct cggatcgggt actgcttcca ggggcgagag agcggaagtc
     4081 ggcgatcgac gaagccggcg gggtacacgt ggtccgcgag gggggacacg ctctccttcg
     4141 agcggaccag cggtgggcac tgcgccggac cgcagaagct gacgcacgag ccgtggaccc
     4201 gcgcccccga aggtgtcatc gccatcgaga ccggcggcga catcggcctc atggacgctg
     4261 gcggcgtcat cgagcagaag ttcttcccgg acacccgtcc caacgggttc cccgactggt
     4321 cccccgatgg ctcgatgatc gccttctcgg gggggagcga gaccggagcc gacgtgtacg
     4381 tgatggacgg cgacggcacc gacgtcacgc tcctcaccgg cacgccggga gacgagatcc
     4441 agcccgcgtg gtcgccggac ggatcacgga tcgcgttcgc gttcgacgat ctgggcgatc
     4501 ccgacttccg ctcaggcatc gcgatcatga acccggacgg ctcggctcgc acggatctgc
     4561 tcatccgcga gaacgagccc gtcggatcgc cgctgtggtc gccggatggg acccgcatcg
     4621 cgttcaccgc cttcagccag gacgggctcc ggccggcgga tgcgtacgtg atggacgcgg
     4681 acgggagcga ggtcaagaag ctcggcgggg ggccgacgtt ggcgctcgcc tggacgcccg
     4741 acggccggca cctcctggtt tcgcgcgagg gttcgctgct catcgtcgaa cccgacggga
     4801 cgcacgagcg tgtggtcctg gcggatcccc cgggaggcgg cgtgctcgag gcggactggt
     4861 cgcccgatgg acggtggatc gtgttgtctg cgcctgaggc gagcggacat ggccagttcg
     4921 acgcgatgta cctcatgcgg gtcgggggcg gcgacgtctt cttcctcggg tccggattcg
     4981 gaccggattg gcgacccgag gctccgtgag cgatcgtccc cggcccgccg agcaccagga
     5041 cggcgcgtcc acgtcacggg aggtaagctt cgaggagctc cagctctcgc agcgcaacca
     5101 cgggatgcac ctcgaggggc tccgctacga cgtgacgccc gccgggatgc actacctctt
     5161 gatccacttc gatgtccccg aggccgagga ggcgctctgg gtcctctcga tcggaggcct
     5221 cgtgcggaat ccgctcgagc cgtcgatgag cgacattcgc tcccgcggca ccgtgacgat
     5281 gccggtcacg atggaatgcg cggggaacgg ccgggcgcgc ctgtcgcctc ggccgatctc
     5341 gcagccgtgg atcaccgagg cgatcgggac cgccgaatgg accggtaccc ccctccgctc
     5401 gatcctcgaa gaggcgggat caacgatgat gccgtcgagc tcgtgttccg cggcgccgac
     5461 cacgggatcc agggcggtgt cgagcaggac tacgagcgca gcctgtcgct cgaggacgcg
     5521 atgcgtgagg aggtgatcct cgcgtacgcg atgaatggcg cgccgcttcc cccgcagcac
     5581 gggttcccgc tccggctcgt ggttcccggc tggtacggga tgacgagcgt gaagtggctc
     5641 accgcgatcg aggccgtcgc cgagcccttc cgcgggttcc agatggaggc gtaccgagtc
     5701 cgtcacgagc ccgacgacga gggcgagccg gtcacgcgga tggccccgcg ggcgttgctg
     5761 gtccctccgg ggttcccgga gttcctgagg cggagccgga tcgtggacgt gggccggcac
     5821 atgctgacgg gccgcgcctg gtcggggcgg gggccgatcg agcgggtcga ggtgagttcg
     5881 gacggtgggg ctacctgggc ggacgccgtc ctcggcgagg ccgtttcgcc ctacgcgtgg
     5941 atgccctgga ccttcaggtg ggacgcggcc gcgcccggag cgttggagct gtgctgccgc
     6001 gcgaccgatg cggctgggaa cacccagccg ctcgagcaac cctggaacct gcacggcttg
     6061 tcgaacaaca tggtgcagcg cgtgccggtc gaggttcgtt cgcccgcgta gcccggctga
     6121 ggctcagagc agcggccgca gctcgtcgaa gcagcggtcg atgtcgtcct ccgtgttgaa
     6181 gaagtgcggg ctgatccgca gtccgtcgcc ccggtagtcg gtgcacacgt cgcgttcgag
     6241 cagcgcgtgg cagatcttcc tggcctcggg cccgacgccg acgttcacga ctcctccgcg
     6301 cgacgcgcgg tcacgcgggg ttcggaccga cagccccaac ttgtcggcgc gggcgatcac
     6361 gtggtcgccg agcacgccct ggcgcgcgcg gatccgttcg ggcgtgacct cggcgatcac
     6421 gtcgaggccg ccctgggcga tgaagaacac cggtgccggc ggcgtgccgt gctcgaaccg
     6481 ccgcgcggtc gggtggtact cgaggtgccg ggtgtcgaac gcgaacggtt cggcggtccc
     6541 gaaccagccg gtcaccatcg gctcgaggga cgcgatcaac ccacgccgca cgtacaggaa
     6601 ggccaggccg ggtccgccgc acaaccactt caggaccccc gccgcgtacg cgtcgcaccc
     6661 cacgtcgtgc aggtcgagcg gcacgatccc gatcccgtgg tagtcgtcga ggaacgacag
     6721 ggcaccgcgc tcgcgcgcga tccggcagac ctccttcgcg tcgacgatcg cgctcgaccg
     6781 gtagagcacg cggttcacca tgacgacggc ggtgcgttcg tcgatcgcgc ggtcgtactc
     6841 ctcgagcggg atcgtcagac catcggggga gcggagccac tcgatctcga cgccgcgtct
     6901 tgcgtgcgcg agccagacgt ggccgtcggt ggggaagtcg agctcggaca tcacgacctt
     6961 ggtgcgttcc ccgaagtcga gcgcggaggc gaacgtcgac atcgcgatcg agatgttcgt
     7021 ggtgatcgcc agctcgtccg cgtcgcaccc ggcgaggcgc gcgaacgacg tcttcagccc
     7081 gcgcatccgg gggaagatgt cctcgaacca cacgtggtcg ggggcgccct tggtggccca
     7141 cgcgtcgatg tacccgtcca ggtaacgccg cgagcgatcc gaagccggcc cgagggacgc
     7201 gctgatcagg taggccttgc gttcgagcac cgggaactcg gatcggtagg acccgaggtc
     7261 acggtcggtc gtcggggtcc tcctcgggtc cg
//
LOCUS       DATBRK010000266         8186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1055077, whole genome shotgun sequence.
ACCESSION   DATBRK010000266 DATBRK010000000
VERSION     DATBRK010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055077"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /gene="cofC"
                     /locus_tag="VIH70_08025"
     CDS             complement(<1..591)
                     /gene="cofC"
                     /locus_tag="VIH70_08025"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEY8116346.1"
                     /translation="MRVVAVPVKSLSRAKTRLSPALTGLERGALTLAMLEDVLDAALS
                     VPGWETWVVSPDEVALEIAAGRGARPIPEAKPPLANAIRQVETKAKDDGATALAVLPA
                     DVPLVTVDTLHEALRTLGAVVLARSADGSGTSLLLRRPPRAIPARFGPDSFRRHVELA
                     EARGLPVSIVERRELSFDVDRPDDILTLLADGRRGRT"
     gene            690..1364
                     /locus_tag="VIH70_08030"
     CDS             690..1364
                     /locus_tag="VIH70_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8116347.1"
                     /translation="MEPWYRAAELALVPPFQLWFNWRLEGLERVPAEGPAIIAGNHLS
                     YLDPFAHGIFVVRAGRRPRFLAKRELFDAPFVGTVLRGAHQISVRRGTGDRSPIDDAA
                     RAVERGEVVVIYPEGTTTTTNEDFSPGRGRTGAVRLSVATGIPILPIATWGGQFVWRK
                     SGRQSLAFGRPIWVAAGEPFDPSERLGDEPDARALRASTDDLMAELGALVDDLRDRYP
                     ARWTPG"
     gene            1374..2576
                     /locus_tag="VIH70_08035"
     CDS             1374..2576
                     /locus_tag="VIH70_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8116348.1"
                     /translation="MPRDHDPEERDPGFATRAIHGQALPPVEQDTPSVPIFQTSTFRF
                     DTSDGFAETINFRRPGFTYTRGYGNPTLLAFERVMAELEGTESALSFASGMAAIHTVA
                     TTLAANGDTIVASNELYGGTYSLFAHVLPRYGIDVRFVDPHDPDAVRGALPGAAMLYV
                     ETIANPNVTVSDLEALGPLASAAGVASVVDNTFASPYLCTPASFGFDYVLHSVTKYIG
                     GHNDLIGGIVCTSEERMAGLRSTVIETGGTLAPLEAWLSMRGLATLQVRMDRHGRTAL
                     ELAGWLERHPKIEAVHYPGLPSHPQHEVARRMLPRGFGGMAAFEVMGGVEAGQRFCDA
                     LELIWVAASLGGIHTLVCHAASTTHRQLDADARRAAGIADGLIRLSVGLEDVEDLIED
                     VDRALEKV"
     gene            2573..3679
                     /locus_tag="VIH70_08040"
     CDS             2573..3679
                     /locus_tag="VIH70_08040"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEY8116349.1"
                     /translation="MRRRVAVVFGGRSAEHEISVLSARSVIGALDPDRYEVIAIGVTK
                     QGRWELLPAGPPGLPAGRPPEALADVAPGSGTEIALDQEPGAQALVSGDGSRTPIDVV
                     FPVLHGPYGEDGSIQGLLEMAGVPYVGAGVLASAVGMDKAVQKVLFAAAGIPGVPYEV
                     VYERDWEEDPEAVEARAAHLGYPLFAKPAALGSSIGIERVDDPSRLRGALETSLRFGR
                     KAVLERAATGARELEVSVLGNDDPVASVAGEIVPRGHTFYDFEAKYLDEHGAELVIPA
                     DVPPDTLEEIQRLAVAAFRAIDAAGMARVDFFLMQDGRVLVNEVNTIPGFTDISMYPR
                     LWEASGLPYPELVARLIELAIERHEAECKRGTGV"
     gene            complement(3708..3941)
                     /locus_tag="VIH70_08045"
     CDS             complement(3708..3941)
                     /locus_tag="VIH70_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY8116350.1"
                     /translation="MVQAYILIQTEVGKAAQVAKEVRDIAGVAEAEDVTGPYDVIVRA
                     EAKNVDELGKLVVAKIQGVEGITRTLTCPVVHL"
     gene            4080..4238
                     /locus_tag="VIH70_08050"
     CDS             4080..4238
                     /locus_tag="VIH70_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116351.1"
                     /translation="MVSKRKRSRRDDDPLAAYRRIRKPMPPPEKVVPDKRRELEDEEA
                     RREIEEER"
     gene            4235..4873
                     /locus_tag="VIH70_08055"
     CDS             4235..4873
                     /locus_tag="VIH70_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011750934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEY8116352.1"
                     /translation="MIFSDRTDAGARLAEALRHLGPNVVVLAIPRGGVIVGDVVAREL
                     GWPLDVVVPRKVGAPGNPELAIGAVAPGIRVLDPRMVHALGVSEPYLEREIATQEAEI
                     ERRQHAYRGGRPPKPVAGRVAVIVDDGVATGSTAVAALRWARAQGAERVVLAVPVAPP
                     QTLERLRAEADEVVVLETPQPFLAVGEWYRDFDQTTDEQVVSALARAAGTRA"
     gene            4870..5193
                     /locus_tag="VIH70_08060"
     CDS             4870..5193
                     /locus_tag="VIH70_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116353.1"
                     /translation="MRVVELVVAGLFALGGLRAIWYWSRRPFEGTDVVDHLLYALYLT
                     GRIGLWFAFAGFFLIYASIQVQGRAALDDLEEFRWYLMVPLVLAALQLLGGWFLGRRS
                     PADDA"
     gene            complement(5197..6708)
                     /locus_tag="VIH70_08065"
     CDS             complement(5197..6708)
                     /locus_tag="VIH70_08065"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEY8116354.1"
                     /translation="MKVEARTVHDNPSADELRRLTEAMPQCRVTEFGNVNVQTRVTSR
                     SSGSTYVVAERSSGRTMTRAAFEEIARLQDDYLRDKDVIEIDGFIGNDPTFRTRARLT
                     IEQANANIAGMQQKLYFPRDDRDEPEVHVIYTPNLEAPGYRDDRVIAVDLEHGVTRVL
                     NSDYFGESKKGGLRMWNAKVYDLGGLALHAGLKVVPTAAGEKVFMIIGLSGTGKTTTT
                     FTTQNDSLPVQDDFVALMPGGKPYGTENGCFAKTFSLDPDFEPNIYNAVVKPTTYLEN
                     VYQDEDGNVNFFEERYTQNGRAVFRMDDLTRFRDSMDVGTVDYLLILNRNENIIPAVA
                     RLDQEQAAAYFMLGETTGTSAGGASEAGKFLRVPGTNPFFPLPHGLQGNRILELLATH
                     PIQTYLLNTGRVGGVDDDDRSKKVKIPHTSACVKGIAERSISWTEDDDFGYEVAEAVP
                     GLDDAELLQPRRLYERQGRLQEYREIVDRLKAERVAHLEGFPELTPEIVKAVG"
     gene            6874..7881
                     /gene="thiL"
                     /locus_tag="VIH70_08070"
     CDS             6874..7881
                     /gene="thiL"
                     /locus_tag="VIH70_08070"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY8116355.1"
                     /translation="MELTEDELVKAVARLLSGGEPGVVVGLGDDAAVVEPGAGQLVLT
                     TDLLAEGVHFERGSISARDLGAKAITVNVSDVAAMAASPRYALMAIAAPGDVDAAWVV
                     ELVGGMRDACAEYALVLVGGDTNRVDLVVVSVAVVGEVAPGRAVLRSGARVGDRIVVT
                     GSLGAAAGGLALSQATTGKAADALSQPWGRALIEALERPVARVGEAQVLARIGATAMM
                     DLSDGLSTDLSRLCEASGVGARVVLGAVPVAETLSRGAEMLEVDASALALSGGEDYEL
                     LATLPGDAVEAARGELRAAFGVDLSEIGEIVEGSAVVAVDAEGRTAPLEPSGWDHFAR
                     G"
     gene            7891..>8186
                     /locus_tag="VIH70_08075"
     CDS             7891..>8186
                     /locus_tag="VIH70_08075"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY8116356.1"
                     /translation="MDDQVRPPRPRALSIAGSDSGGGAGIQADLKTFSALGVFGMTAI
                     TAVTIQNTRGVSGYETLPPQVVADQIRAVVTDIGVDAAKTGMLASAQIVEAVAEA"
BASE COUNT         1125 a         2776 c         3044 g         1241 t
ORIGIN      
        1 ggtccgccct cgccggccgt cggcgagcag cgtgaggata tcgtcggggc gatcgacgtc
       61 gaacgacagc tcgcggcgct cgacgatgga gaccgggagg ccacgcgcct cggcgagctc
      121 cacgtgccgt cggaacgagt cgggcccgaa cctggcgggg atcgctcgcg gcgggcgccg
      181 caggagcagg ctggttcccg acccgtccgc cgagcgcgcg aggacgaccg ctccgagcgt
      241 ccgaagggct tcgtggagcg tgtcgacggt cacgagcgga acgtcggccg gcagcaccgc
      301 gagcgcggtc gcgccgtcgt ccttcgcctt cgtctcgacc tgccggatcg cgttcgcgag
      361 gggaggcttg gcctcgggga tcggtcgggc gccccggccg gccgcgatct cgagcgccac
      421 ctcgtcgggc gagaccaccc aggtctccca gccgggcacg gacagggccg cgtccaggac
      481 gtcctcgagc atcgcgagcg tgagcgcgcc gcgctcgagc cccgtcaggg cgggggacag
      541 ccgcgttttc gcgcgcgaga gcgacttcac cggcacggcc acgacgcgca tgagcagagg
      601 ctagtaccgc ggcggtgtaa cgaacgcagg accgtgagat cgactcgcgg agctacgcgg
      661 tagcatcgcc gccccgaagg aggcgcgtcg tggaaccgtg gtaccgagcg gccgagctcg
      721 cgttggtccc gccgttccag ctctggttca actggcggtt ggaagggctg gagagggttc
      781 ccgcggaggg gccggccatc atcgcgggga accacctctc ctacctggac ccgttcgcgc
      841 acggcatctt cgtggtcagg gccgggcgcc gaccccggtt cctcgccaag cgggagctgt
      901 tcgacgcccc gttcgtcggg accgtgctgc gaggggcgca ccagatctcg gtccggcgcg
      961 ggaccggcga ccggtcgccg atcgacgacg cggctcgcgc ggtcgagcgg ggggaggtgg
     1021 tcgtgatcta cccggagggg acgaccacca cgacgaacga ggacttctca ccgggccgtg
     1081 gcaggaccgg cgccgtccgg ctgtcggtcg ccaccggcat cccgatcctc ccgatcgcca
     1141 cgtggggggg tcagttcgtc tggcgcaagt cggggcggca gagcctcgcg ttcggacggc
     1201 cgatctgggt ggccgcgggt gagccgttcg acccgagcga acggctgggg gacgagcccg
     1261 acgctcgggc gctccgcgca tccaccgacg acctgatggc cgagctcggc gcgctcgtgg
     1321 acgacctccg cgaccgatat cccgcgcgct ggaccccggg gtaggctggg gccgtgccgc
     1381 gcgaccacga tccggaggaa cgcgatccgg ggttcgccac gcgtgcgatc cacggccagg
     1441 cgctgccgcc ggtcgagcag gacacaccca gcgtcccgat cttccagacg tccacgttcc
     1501 ggttcgacac ctccgacgga ttcgccgaga cgatcaactt ccgccggccc gggttcacgt
     1561 acacgcgcgg ctacgggaac ccgacgctcc tcgcgttcga acgcgtgatg gccgagctcg
     1621 aggggaccga gtcggcgctg tcgttcgcgt cggggatggc ggcgatccac accgtggcca
     1681 cgacgctggc cgcgaacggc gacacgatcg tggcgagcaa tgagctgtac ggcggcacct
     1741 actcgttgtt cgcgcacgtg ctgccgcgct acgggatcga cgtgcggttc gtggacccgc
     1801 acgatccgga cgcggtccgg ggggcgctcc ccggcgcggc gatgctctac gtcgagacga
     1861 tcgcgaaccc gaacgtgacg gtgtccgacc tcgaggcgct cgggccgctc gcgtcggccg
     1921 ccggcgtcgc gagcgtggtc gacaacacgt tcgcgtcgcc gtatctgtgc acccccgcgt
     1981 cgttcgggtt cgactacgtc ctgcactcgg tgaccaagta catcgggggc cacaacgatc
     2041 tgatcggcgg catcgtctgc acgtccgagg agcggatggc ggggttgcgc tccacggtga
     2101 tcgagaccgg cgggacgctc gcaccgctcg aggcgtggct gtcgatgcgt ggcctggcga
     2161 cgctgcaggt gaggatggac cgccacggcc ggacggcgct cgagctcgcc gggtggctcg
     2221 agcgccaccc caagatcgag gccgtgcact accccgggct cccgtcccac ccgcagcacg
     2281 aggtggcgcg gcggatgctt cctcgcgggt tcggagggat ggcggcgttc gaggtcatgg
     2341 gcggggtcga ggccgggcag cgcttctgcg acgcgctcga gctgatatgg gtggccgcga
     2401 gcctcggggg gatccacacg ctcgtgtgtc acgccgcctc gacgacccac cggcagctcg
     2461 acgccgacgc ccgcagggcg gccggcatcg ccgacggtct catccggctg agcgtgggtc
     2521 tggaggacgt cgaggacctg atcgaggacg tcgaccgcgc gctggagaag gtgtgaggcg
     2581 gcgcgtcgcc gtcgtcttcg ggggtcgctc ggccgaacac gagatcagcg tgctctcggc
     2641 ccgatccgtg atcggcgcgc tcgacccgga ccggtacgag gtgatcgcga tcggggtgac
     2701 gaaacagggt cggtgggagc tcctgccggc gggtccgccc ggactgcccg cggggaggcc
     2761 gccggaggcg ctcgccgacg tcgcgccggg ttcgggcacc gagatcgcgc tcgaccagga
     2821 gcccggagcc caggcgctgg tgtcggggga cggttcgcga acgccgatcg acgtcgtctt
     2881 cccggtgctg cacggcccgt acggggagga cgggtcgatc cagggcttgc tcgagatggc
     2941 cggcgtgccc tacgtcggtg cgggcgtgct ggcctcggcg gtggggatgg acaaggcggt
     3001 gcagaaggtg ctgttcgccg ccgcggggat ccccggcgtg ccgtacgagg tcgtgtacga
     3061 gcgcgactgg gaggaggatc ccgaggccgt cgaggcgcgc gccgcgcacc tcgggtaccc
     3121 gctgttcgcc aagcctgcgg cgctcggctc ctcgatcggg atcgagcgtg tcgacgaccc
     3181 gtcgcggctg cgcggcgccc tggagacgtc cctccggttc ggccgcaagg cggtgctcga
     3241 acgcgcggcc accggcgcgc gcgagctcga ggtctcggtg ctcggcaacg acgatccggt
     3301 ggcctcggtc gcgggcgaga tcgtccctcg gggtcacacg ttctacgatt tcgaggccaa
     3361 gtacctcgac gagcacggcg ccgagctggt gatcccggcc gacgtcccgc cggatacgct
     3421 cgaggagatc cagcgcctgg cggtcgccgc gttccgggcg atcgacgcgg cggggatggc
     3481 gcgggtcgac ttcttcctga tgcaggacgg gcgcgtgttg gtcaacgagg tcaacacgat
     3541 ccctgggttc accgacatct cgatgtaccc gcggctgtgg gaggcctcgg gcctgcccta
     3601 ccccgaactc gtcgcccggc tgatcgagct cgcgatcgag cgccacgagg ccgaatgcaa
     3661 gcggggaacg ggggtctgac gccgcgccga ccgaagccgg ctcgcgtcta caagtggacg
     3721 acggggcagg tgagcgtccg ggtgatcccc tcgacaccct ggatcttcgc gacgaccagc
     3781 ttgcccagct cgtcgacgtt cttcgcctcg gcgcgcacga tcacgtcgta gggtcccgtc
     3841 acgtcctcgg cctcggcgac gccggcgatg tcgcggacct ccttggcgac ctgggcggcc
     3901 ttgccgacct cggtctggat caggatgtac gcctggacca cgctggacct ccctgttcgc
     3961 gaagcgctca tgctatgcgg gcacccgaag cccgacaaac gctcgtccgc gccgcaagac
     4021 ggggatgcgt gagggcgctc gtccggtcat ccgtgtgagc ggagttccat aatcgagggg
     4081 tggtctccaa gcggaagcgg tccaggcgcg acgacgaccc gctcgccgcc taccggcgga
     4141 tccgcaagcc gatgccgccg cccgagaagg tcgtccccga caagcggcgc gagctcgagg
     4201 acgaggaggc ccggcgcgag atcgaggagg agcggtgatc ttctccgacc gaacggacgc
     4261 gggtgcacgg ctcgcggagg cgctccgcca cctcggaccc aacgtcgtgg tgctcgcgat
     4321 cccgagaggt ggcgtgatcg tcggtgacgt ggtcgcgcgg gagctcggct ggccgctcga
     4381 cgtggtcgtc ccgcgcaagg tgggggcgcc gggcaacccc gagctcgcga tcggcgcggt
     4441 ggcacccggg atcagggtgc tggatccgag gatggtgcac gcgctcggcg tgagcgagcc
     4501 ctacctcgaa cgagagatcg cgacgcagga ggcggagatc gagcgccgcc agcacgccta
     4561 ccgtggtggc cggccgccga aacccgtcgc gggtcgcgtc gcggtgatcg tcgacgacgg
     4621 cgtggcgacc ggcagcacgg ccgtggccgc gctccggtgg gcgcgggcgc agggcgccga
     4681 acgggtcgtg ctcgcggtgc cggtggcgcc gccacagacg ctcgagcgcc tccgcgccga
     4741 ggccgacgag gtagtcgtgc tcgaaacacc gcagccgttc ctcgcggtgg gcgagtggta
     4801 ccgggacttc gaccagacca ccgacgagca ggtcgtctcg gcgctcgccc gcgcggcggg
     4861 cacccgcgcg tgagggtcgt cgaactcgtg gtcgcggggc tgttcgcgct cggtggcctg
     4921 cgcgcgatct ggtactggag ccgacggccg ttcgagggca ccgacgtcgt cgaccacctg
     4981 ctctacgcgc tgtacctcac gggccggatc ggcctgtggt tcgcgttcgc cggcttcttc
     5041 ctgatctacg cctcgatcca ggtccagggc cgcgcggcgc tggacgatct cgaggagttc
     5101 cggtggtacc tgatggtccc gctggtcctc gcggccttgc agctgctggg cggttggttc
     5161 ctgggccggc gctcgccggc ggacgatgcc tgaggtttac ccgaccgcct tgacgatctc
     5221 gggcgtgagc tcggggaacc cttcgagatg cgcgacgcgt tcggccttca ggcgatcgac
     5281 gatctcccgg tactcctgca ggcgcccctg ccgctcgtag aggcgacgcg gttgcaggag
     5341 ctccgcatcg tccaggcccg ggacagcctc ggccacctcg tacccgaagt catcgtcctc
     5401 ggtccacgag atcgatcgct cggcgatgcc cttcacgcac gccgaggtgt gcgggatctt
     5461 caccttcttg gagcgatcgt cgtcgtccac gcccccgacg cgtcccgtgt tcaacaggta
     5521 cgtctggatc gggtgcgtcg cgagcagctc gaggatccgg ttgccctgca gcccgtgcgg
     5581 caacgggaag aaggggttgg tgccggggac ccgcaggaac ttgccggcct cggacgctcc
     5641 tccggcggag gtgccggtcg tctcgccgag catgaagtag gcggcggcct gctcctgatc
     5701 gagcctggcg accgcgggga tgatgttttc gttccggttg aggatcagca gatagtccac
     5761 ggtcccgacg tccatcgagt cgcggaaccg ggtgaggtcg tccatgcgga acaccgcgcg
     5821 cccgttctgc gtgtagcgct cctcgaagaa gttcacgttg ccgtcttcgt cctggtacac
     5881 gttctcgagg taggtcgtcg gcttgacgac cgcgttgtag atgttgggct cgaagtccgg
     5941 atcgaggctg aacgtcttcg cgaagcaccc gttctcggtc ccgtagggct tgccgccggg
     6001 catgagcgcg acgaaatcgt cctgcacggg caacgagtcg ttctgcgtcg tgaacgtcgt
     6061 ggtcgtcttc ccggtgcccg agagcccgat gatcatgaac accttctcgc cggcggccgt
     6121 cgggacgacc ttcagcccgg cgtgcagggc caggccgccg aggtcgtaga ccttcgcgtt
     6181 ccacatccgt aggccgccct tcttcgactc gccgaaatag tcggagttca ggacgcgggt
     6241 cacaccgtgc tcgagatcga cggcgatcac gcggtcgtcc cggtagccgg gcgcctcgag
     6301 gttcggcgtg tagatcacgt gcacctccgg ctcgtcgcga tcgtcgcgcg ggaaatagag
     6361 cttctgctgc atcccggcga tgttcgcgtt cgcctgctcg atcgtgaggc gtgcccgcgt
     6421 ccggaaggtc ggatcgttgc cgatgaagcc gtcgatctcg atgacgtcct tgtcgcggag
     6481 gtagtcgtcc tgaagccggg cgatctcctc gaacgccgcc cgcgtcatcg tccggccgct
     6541 cgagcgttcg gcgaccacgt acgtgctgcc ggagctcctc gacgtgaccc gggtctggac
     6601 gttcacgttg ccgaactccg tgacccggca ctgcggcatc gcctccgtga gacggcggag
     6661 ctcgtccgcg gacgggttgt cgtggacggt gcgtgcctcg accttcaacg gtgcgctcct
     6721 tcgtcgggaa ggctcgcgag ctcgggccgc tcggagcggc ccttatccta cccaaggcga
     6781 ggcggccgac cgccgccgcg acgggccgcc ggggccgaag gagcggaccc aggaacgggc
     6841 tcaaggaacg gcctaaggaa aacggaagga cccatggaac tgaccgaaga cgagctggtc
     6901 aaggccgtgg cgcgcctgct ctccgggggc gagccgggtg tcgtggtggg tctgggcgac
     6961 gacgccgcgg tcgtcgaacc cggcgccggc cagctcgtgc tcacgacgga cctcctcgcc
     7021 gagggcgtcc acttcgagcg cggatcgatc tcggcgcggg acctcggcgc caaggcgatc
     7081 accgtgaacg tctcggacgt ggcggcgatg gcggcgagcc cccggtacgc gctcatggcg
     7141 atcgcggctc cgggcgacgt cgacgcggcc tgggtggtgg aactggtcgg cggaatgcgt
     7201 gacgcgtgcg cggagtacgc gctcgtgctc gtcggcggcg acacgaaccg ggtggacctc
     7261 gtcgtcgtat cggtggccgt ggttggggag gtcgcgccgg gccgcgcggt gctgcgttcg
     7321 ggcgcccgcg tgggggaccg gatcgtggtg accggctcgc tcggcgcggc tgcgggcggc
     7381 ctcgcgctct cgcaagctac gaccggcaaa gcggccgacg ccctgtcgca gccgtggggc
     7441 cgcgcgctga tcgaggcgct cgagcgaccc gtcgcgcgcg tcggagaggc gcaggtgctc
     7501 gcgcggatcg gcgcgaccgc gatgatggat ctgtccgacg gcctgtcgac cgacctgtcg
     7561 cgactctgcg aggcaagcgg cgtcggcgcg cgcgtcgtcc tcggcgccgt ccccgtcgcc
     7621 gaaaccctgt cgaggggcgc ggagatgctc gaggtcgacg cctcggcgct cgcgctctcc
     7681 ggaggcgagg actacgagct gctcgcgacg ctcccggggg acgccgtcga ggccgcccgc
     7741 ggggagctgc gggcggcgtt cggcgtcgac ctgtccgaga tcggcgagat cgtggaggga
     7801 tcggccgtcg tcgccgtgga cgccgagggc cggacggcgc cactcgagcc ctcgggctgg
     7861 gatcacttcg cccgggggta gcctggtctc gtggacgatc aggttcgacc gccacgacca
     7921 agagcgctct cgatcgccgg ttcggactcg ggcggcggcg ccgggatcca ggccgacctg
     7981 aagacgttca gcgcgctcgg cgtgttcggg atgaccgcga tcaccgccgt cacgatccag
     8041 aacacccgag gcgtcagcgg gtacgaaacg ctcccaccgc aggtcgtcgc cgaccagatc
     8101 cgtgcggtcg tcacggacat cggggtcgat gcggccaaga ccgggatgct ggcctccgcg
     8161 cagatcgtcg aggcggtcgc cgaggc
//
LOCUS       DATBRK010000267         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_764334, whole genome shotgun sequence.
ACCESSION   DATBRK010000267 DATBRK010000000
VERSION     DATBRK010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764334"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="VIH70_08080"
     CDS             complement(<1..1571)
                     /locus_tag="VIH70_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8116357.1"
                     /translation="MSQVLERSAEILRPYAPRLLIRWLAEQPHRSFRTLDGSMVFVDI
                     SGFTKMSERLARHGKVGAEEVTEVLGAVFAKLLGVAYGEDGSLLKFGGDALLLWFSGS
                     GHAQRAATSAHGMRAALRSVGRLDTTAGKVLLRMSVGVNTGSFHFFLVGESHRELIVT
                     GPAASRTVEMESTATAGEILVSRDTAAALPARSLGQPKREGILLRSGPPTLSFGRDDV
                     EVLISSIDVAQGIPVTLREHLLSGATEPEHRTATVAFVHFDGVDGLIEQQGVDVTAAG
                     LHELIACVQRAAGDNGVTFLGTDIDHDGGKVILVAGAPNALGDDEGRMLLTLRSVIDA
                     DTTLPIRIGVNKGPVFAGDIGPGYRRTYTVMGDAVNLAARVMSMAVPGQILATGPVLD
                     ASSVAFNTVALEPFTVKGKKQPVHAFMVGHSTGSKPETVSREFPLIGRDAEMDAIRRA
                     LLELRGGHGSVIELVGNAGMGKTRLLSEFRAEAPDLPNLVSGCEPYESSVAYLPIRRL
                     LRLLLGAGQDVSGLA"
     gene            1696..2166
                     /locus_tag="VIH70_08085"
     CDS             1696..2166
                     /locus_tag="VIH70_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116358.1"
                     /translation="MLERDDDLSPTLAETARRRADLRDALVEVEQASSRPAVGREPEW
                     TKEVLLRLEGLAHAIDEHIQITERTEGLYDEISAKAPHLSTKIDALREEHPELRERAT
                     ALITRLKTTPVGPAWPLREARDDLQRLLGQIVRHRQLGSDLVWEAYNLDIGGIE"
     gene            2194..>3197
                     /locus_tag="VIH70_08090"
     CDS             2194..>3197
                     /locus_tag="VIH70_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY8116359.1"
                     /translation="MRIALGQVDTTVGDLPGNVDLLTAWASRGTEADADVVVFPELAI
                     TGYPPEDLVLRPSFVDDNLIALDELAAKTAGGASIVVGFVDRTEAGIHNAAAVIADGG
                     VIARYHKIKLPNYGVFDEARTFTPGDAACPIRLADSELGLSVCEDAWMPGPPFDEYAR
                     RKTPVILNINGSPFHRGKAEERVEICRERALETGSWIVYVNAVGGQDELVFDGGSVVV
                     RPDGSLAHHAALFEEDLLVVDIDGETSSAQGRPPWPEDPEQVYLGLVLGLRDYVRKNG
                     FEEVVLGLSGGIDSAFVAVLARDALGAEAVRALAMPSRVSSPESLEDARQVATGLGIR
                     L"
BASE COUNT          490 a         1132 c         1119 g          456 t
ORIGIN      
        1 tgcgcgagcc cggacacgtc ctggccggcc cccagcagca gccgcaggag gcgtcggatc
       61 ggcaggtagg cgaccgagga ctcgtagggc tcgcacccgg acaccaggtt ggggaggtcg
      121 ggcgcctcgg cccggaactc gctcagcagc ctcgtcttgc ccatccccgc gttcccgacc
      181 agctcgatga ccgagccgtg cccgccccgg agctccagga gcgcgcggcg gatcgcgtcc
      241 atctccgcgt cgcgaccgat cagggggaac tcgcgcgaga cggtctccgg cttgctcccg
      301 gtcgagtgcc ccaccatgaa ggcgtgcacc ggttgcttct tgcccttcac cgtgaacggc
      361 tcgagcgcca cggtgttgaa cgcgacggac gaggcgtcga ggaccggtcc ggtcgcgagg
      421 atctgtcccg gcacggccat cgacatcaca cgcgcggcca ggttcacggc gtcgcccatc
      481 acggtgtacg tgcgccggta cccgggcccg atgtcgccgg cgaagacggg acccttgttc
      541 acaccgatcc ggatgggcag ggtcgtgtcc gcgtcgatga ccgagcgaag cgtgagcagc
      601 atccggccct cgtcgtcgcc gagggcgttg ggcgcacccg ccaccaggat gaccttccca
      661 ccgtcgtgat cgatgtcggt ccccaagaac gtgaccccgt tgtctcccgc ggcgcgctgc
      721 acgcaggcga tgagctcgtg caggcctgcg gccgttacgt ccaccccctg ctgctcgatc
      781 aggccgtcga cgccgtcgaa gtgcacgaac gcgacggtcg cggtgcggtg ctcgggttct
      841 gtggccccgg agagcagatg ctcacggagc gtcacgggga tcccctgcgc cacgtcgatg
      901 gaggagatca ggacctccac gtcgtcgcgt ccgaacgaca gggtcggcgg cccgctgcgg
      961 agcaggatgc cctcacgctt gggctgtccg agcgatctgg ccggcagcgc cgcggccgtg
     1021 tcacggctca cgaggatctc gcccgcggtc gccgtggact ccatctcgac ggttcggctc
     1081 gccgccggac ccgtgacgat gagctctcga tgcgactcgc ccacgaggaa gaaatggaag
     1141 gaaccggtgt tcacccccac ggacatccgc aggagcacct tgccggcggt cgtgtcgaga
     1201 cgaccgacgc tgcgaagcgc cgcccgcatc ccgtgggcgg aggtggcggc cctctgggcg
     1261 tgcccggacc ccgagaacca gagcaggagc gcgtcgcccc cgaacttcag gaggctgccg
     1321 tcctccccgt aggcgacccc gagcagcttg gcgaacacgg ccccgaggac ctcggtgacc
     1381 tcctcggcgc ccaccttccc gtggcgggcc aggcgctcgg acatcttcgt gaacccggag
     1441 atgtccacga agaccatcga gccgtcgagc gttcggaacg accgatgcgg ctgttcggcg
     1501 agccaacgga tgaggagccg gggagcgtac ggcctcagga tctcggcgct ccgctcaagc
     1561 acctgcgaca ccaacgttcc tcctcggggc ggggcgacga gtgtacgcgg ttgttcggcc
     1621 ccgagcgcgc gccggtcgtg cgcgagcctc ggccccgctt gctcgccgag ccgtttgccg
     1681 cctaaggttc tcgggatgct cgaacgcgac gacgacctga gcccgaccct cgccgagacc
     1741 gcccgccggc gcgcggacct gcgcgacgcg ttggtggagg tcgagcaggc gagctcgcga
     1801 cccgccgtcg ggcgcgagcc cgagtggacc aaggaggtcc tgcttcgcct cgagggactc
     1861 gcgcacgcca tcgacgagca catccagatc acggagcgaa ccgagggcct gtacgacgag
     1921 atctccgcca aggcccccca cctgtccacg aagatcgacg cgctccgcga ggagcacccc
     1981 gagctgcgcg agcgcgcgac ggccctgatc acccggctca agacgacgcc cgtcggaccc
     2041 gcgtggccgc tgcgcgaggc cagggacgac ctccagcggc tgctcggcca gatcgtgcgc
     2101 catcgccagc tcggatcgga tctggtctgg gaggcctaca acctcgatat cggcgggatc
     2161 gagtagcccc ggcctccgtc taggctgacg ctcgtgagga tcgcgctcgg acaggtcgac
     2221 acgaccgtcg gtgatctacc cggcaacgtc gacctcctga ccgcctgggc gtcccggggg
     2281 acggaggccg acgccgacgt cgtcgtgttc ccggagctcg cgatcacggg ctacccaccc
     2341 gaggacctgg tactgcgccc gtcgttcgtc gacgacaacc tgatcgccct ggacgagctg
     2401 gcagccaaga cggcgggcgg agcctcgatc gttgtcgggt tcgtcgaccg gaccgaggca
     2461 ggcatccaca acgctgcggc ggtcatcgcc gacggcgggg tgatcgcgcg ataccacaag
     2521 atcaagctcc cgaactacgg cgtgttcgac gaggcgcgga cgttcacgcc gggcgacgcc
     2581 gcgtgcccga tccggctcgc cgactccgag ctcgggctgt cggtgtgtga ggacgcgtgg
     2641 atgccgggtc caccgttcga cgagtacgcg cggcggaaga ctcccgtcat cctcaacatc
     2701 aacgggtcgc cgttccaccg cggcaaggcc gaggagcgcg tcgagatctg cagggagcgg
     2761 gcgctcgaga ccggatcgtg gatcgtctac gtgaacgcgg tcggcggaca ggacgagctc
     2821 gtgttcgacg gtgggagcgt ggtcgtccgg ccggacggat cgctcgcaca ccacgcggcg
     2881 ctgttcgagg aggacctcct cgtcgtcgac atcgacggcg agacctccag cgcccaggga
     2941 cgcccgccgt ggcccgagga tccggagcag gtgtatctcg gactcgtcct cgggctgcgg
     3001 gactacgtcc gcaagaacgg cttcgaggag gtcgtgctcg gtctgtcggg cgggatcgac
     3061 tcggcgttcg tcgccgtgct cgcccgggac gcgctcggcg ccgaggcggt acgcgcgctc
     3121 gcgatgccgt ccagggtctc ctcgcccgag agcctcgagg acgcgaggca ggtggcgacc
     3181 gggctcggga tccggct
//
LOCUS       DATBRK010000268         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1634403, whole genome shotgun sequence.
ACCESSION   DATBRK010000268 DATBRK010000000
VERSION     DATBRK010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634403"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..786
                     /gene="msrA"
                     /locus_tag="VIH70_08095"
     CDS             265..786
                     /gene="msrA"
                     /locus_tag="VIH70_08095"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY8116360.1"
                     /translation="MSTETATFGAGCFWGVEYVFERVPGVLRTEVGYAGGHTENPTYR
                     DVCSHGTGHAEVLRVEFDPSVVSFEQLLEVFWAMHDPTQVNRQGPDVGDQYRSAIFTS
                     SDEQRRAAEASRDAAQARFDRPIATEITPAGTFYPAEDYHQRYYDKNGHEPYCHVIPW
                     ATLRELGLVKAGA"
     gene            complement(796..1143)
                     /locus_tag="VIH70_08100"
     CDS             complement(796..1143)
                     /locus_tag="VIH70_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY8116361.1"
                     /translation="MGRDLASMECVPCRGGVPPLDRVEIESYLADLGHEWQVVDDHHL
                     EKEYRFADFKEALAFTNRVGDLAERIGHHPDIYLAWGRVRLTVWTHKIDGLHEADFVF
                     AAKADRALADPAG"
     gene            complement(1143..>1646)
                     /locus_tag="VIH70_08105"
     CDS             complement(1143..>1646)
                     /locus_tag="VIH70_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase family protein"
                     /protein_id="HEY8116362.1"
                     /translation="AVGEPLARELVRHGARLVVTDIDDVRAKELAQELDAETAGSEEI
                     YDALCDVFVPCATGGVLSRETIPRLRCRVIAGAANNQMAEPEDADRLAERGILYAPDY
                     VVNAGGMIHLASLELLGEDVAARDERLRGIGDSLAEVFALAERRGISTAAAADAIVEE
                     RLAAGRT"
BASE COUNT          255 a          606 c          549 g          236 t
ORIGIN      
        1 gtccccatag aggacgacgt actcacgtac cgttccgtca ctgccggcac tggcaggaac
       61 ggcagccagg agtcccccgg ctaccaacgc cacgacggca gcgatgacca tgcgactacg
      121 catgggcacc ccctttctcc gacccgcccg ctacccaccc gggcgccgct caaacctggc
      181 gccatgcatt cctcggtccg acgtaacgtt gtgggagctg gacggacaga agaggtgaac
      241 ccatccgaag gagggatgct cagcatgtcc accgagaccg cgacgttcgg cgccgggtgc
      301 ttctggggcg tcgagtacgt gttcgagcgt gtccccgggg tcctacggac cgaggtcggc
      361 tacgcgggcg gccacacgga gaaccccacc taccgtgacg tgtgctcgca cgggaccggc
      421 cacgccgagg tgctccgcgt cgagttcgac ccttcggtcg tgagcttcga gcagctgctc
      481 gaggtgttct gggcgatgca cgacccgacc caggtgaacc gccagggtcc ggacgtcggc
      541 gaccagtacc ggagcgcgat cttcacgagc tcggacgagc agcggcgggc cgccgaggcc
      601 tctcgtgacg ccgcccaggc gcggttcgac cggccgatcg cgaccgagat cacgcccgcg
      661 ggaacgttct acccggcgga ggactaccac cagcggtact acgacaagaa cggccacgag
      721 ccgtactgcc acgtgatccc gtgggccacc ctgcgcgagc tcggcctcgt gaaggccggc
      781 gcctagccga acccctcagc cggccgggtc cgccagggcc cggtcggcct tcgccgcgaa
      841 cacgaagtcg gcctcgtgca gcccgtcgat cttgtgggtc cacacggtca gccggaccct
      901 gccccacgcg aggtagatgt ccgggtggtg accgatccgc tccgccaggt cgccgacgcg
      961 gttggtgaac gcgagcgcct ccttgaaatc cgcgaagcgg tactccttct ccaggtgatg
     1021 gtcgtccacg acctgccact cgtgtccgag atcggccagg tacgactcga tctcgactcg
     1081 gtcgagcggc ggcaccccgc cgcggcacgg gacgcactcc atcgaggcca ggtcgcgacc
     1141 catcacgtcc tgcccgcggc caggcgttcc tcgacgatcg cgtcggcggc ggccgccgtc
     1201 gagatccctc gccgctcggc gagagcgaac acctccgcga gggagtcgcc gatgccccgc
     1261 aggcgttcgt cgcgcgcggc tacgtcctcg ccgagcagct cgagcgatgc gagatggatc
     1321 atgccgccgg cgttgaccac gtagtcgggc gcgtacagga tgccgcgctc cgcgaggcgg
     1381 tccgcgtcct cgggctcggc catctggttg ttcgcggcgc cggcgatcac gcggcatcgg
     1441 agccgcggga tcgtctcccg cgagagcacg cccccggtcg cgcacggcac gaagacgtcg
     1501 cacaacgcgt cgtagatctc ctcgctcccg gcggtctcgg cgtccagctc ctgcgcgagc
     1561 tccttcgcac gaacgtcgtc gatgtcggtc acgaccaggc gggcgccgtg ccggacgagc
     1621 tcgcgcgcga ggggctcgcc caccgc
//
LOCUS       DATBRK010000269         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_329027, whole genome shotgun sequence.
ACCESSION   DATBRK010000269 DATBRK010000000
VERSION     DATBRK010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329027"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VIH70_08110"
     CDS             <1..260
                     /locus_tag="VIH70_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116363.1"
                     /translation="MKGLPDDRCQCPHWGYVFKGKMTARYADRDEVFEAGDAFYTPPG
                     HVPVENEPGTEILWFSPSEELRTAEAVMMKNMQAMQDGSTP"
     gene            complement(671..1900)
                     /locus_tag="VIH70_08115"
     CDS             complement(671..1900)
                     /locus_tag="VIH70_08115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8116364.1"
                     /translation="MIVGGGKAGGTAAATLRDEGFNGPVVLVSREPGIPFGRPPLSKT
                     YLRSEEDLTDWYVRPPDWYEAHDVDLRPDSVVALDPAAHALALRSGRELGYEKLLIAT
                     GGQNRRLELPGAELPGIHYLRTVAECDAIKREAEAGRRAVVVGMGFVGCEVAASLTQL
                     GVQVTAVFPGRNPLERVLGEQLGDLIGEVHRSKGVRLLPGAQVVAFHGTERLESVVLA
                     DGEQVGCDFAVVGVGIDPDVPAIAGSSVAQDNGILVDERCRSSAADVYAAGDVANHLH
                     PILGRIRVEHYNTAEHHGAAAARAMLGSAAPFDYVHNFFSDQYEHILQYVGHATTWDD
                     FAVRGSLRDRKFVGFYLVGGVVRAAVGFDRGGDPEWEPDSEMAASARLVASRALPGRG
                     VLTDEGADLRSLASATG"
     gene            complement(2180..2275)
                     /locus_tag="VIH70_08120"
                     /pseudo
     CDS             complement(2180..2275)
                     /locus_tag="VIH70_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            2307..3311
                     /locus_tag="VIH70_08125"
     CDS             2307..3311
                     /locus_tag="VIH70_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8116365.1"
                     /translation="MRYMILVHPSPGSESGAMPTEQQIAEQLAFHQAIDEAGILIDAM
                     GFHPTSDAWRVQQRTDGSREVIEGPFHDEHLIAGYTLIDVASREEAMDWSLRYAKTAT
                     EDEDGEIEVRRLFDLDEFVQGPAVRGFRELDLVGRIAADVGRSRPDLSRAVSPDGTVT
                     VLFTDIEGSTQVTEALGDAEWIRVLRAHNALVRDQVAAHSGIEVKSQGDGFMLAFASA
                     KDALLCAIGIQRALAQAPSDGHRLRVRIGLHTGEMIREEDDFFGKAVVLAARIAAEAR
                     GGEILVSRLVRELTEGASLFSFTDPTDVELKGLSGMHSLWAVRWQTAMSGEALAASQR
                     "
     gene            complement(3896..>4232)
                     /locus_tag="VIH70_08130"
     CDS             complement(3896..>4232)
                     /locus_tag="VIH70_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010934851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116366.1"
                     /translation="IAGHLEGVNEGATYSETSAAARAEGLDPDVAAELQAKADTLFKG
                     ESLRAMLLNAYGWWTLATIALWAGLAMVIAGLILGIFSILGFRHAGRAEAKSVERRVS
                     TPEYAGASS"
BASE COUNT          670 a         1428 c         1419 g          715 t
ORIGIN      
        1 tcatgaaggg gcttccggac gaccgctgcc agtgcccgca ttgggggtac gtcttcaagg
       61 gcaagatgac cgctcggtac gccgaccgtg atgaggtatt cgaggccggc gacgccttct
      121 acacgccgcc cggccacgtc ccggtggaga acgagcccgg cactgagatc ttgtggttca
      181 gcccgagcga agagctgcgc acggccgagg cggtcatgat gaagaacatg caggcgatgc
      241 aggacggctc aacgccttag gcgaaggggc gcgcggcgac cacgcctgag aggcttccta
      301 tcggactctg tagtcgaggt tgcttggccg tgacgttcga aggttggccg cgagacaaat
      361 gcgggacgac aaatgaacct gctcgacctc gccgttcgcg cagggctcct gacttccggg
      421 ttcttggtag tcccaacgca gttcgaacca gtgtctccac cttgagagtg ctcggggtcg
      481 actccgctac ctgcggcgat gcccgaatcc ggtacctgac ctgcggattc aatgctccga
      541 ccgttcccgc cgtttcttga cgtctcccgg tcgttcgcgg gacgaatgcg ggacgcgcgt
      601 agtcgctcgg ccgacggcca ggcggttcga gatgggttgt gtggttgcac tagacgcacc
      661 ctcggcgtgc ctacccggtc gcgctggcta gagatcggag gtcagcgccc tcgtcggtga
      721 gcacgccgcg gccgggcagt gcgcggctgg ccaccaggcg cgcgctggcc gccatttcgg
      781 agtcgggctc ccactccgga tcgcccccgc ggtcgaagcc gacggccgcc cgcacgaccc
      841 cgccgaccag gtaaaaaccg acgaacttgc ggtcccgtag gctcccgcgg acggcgaagt
      901 cgtcccaggt cgtagcgtgg ccgacgtact gcaggatgtg ctcgtactgg tcggagaaga
      961 agttgtggac gtagtcgaag ggggcggccg agcccagcat cgcccgtgcc gccgcggcgc
     1021 cgtggtgctc ggcggtgttg tagtgctcca cccggatgcg gccaaggatc gggtgcaggt
     1081 ggttggcgac gtcgccggcc gcgtagacgt cggccgcgct gctgcggcac cgctcgtcca
     1141 ccaggatccc gttgtcctgg gccacggacg agcccgcgat cgcgggcacg tcagggtcga
     1201 tcccgacgcc gacgacggcg aagtcgcacc cgacctgctc cccgtcagcc agcacgaccg
     1261 actcgagccg ctcggtcccg tggaacgcca cgacctgagc tccggggagc agcctgactc
     1321 cctttgagcg gtgaacctca ccgatgaggt cgccgagctg ttcgcccagg acccgttcca
     1381 gcgggttccg gccgggaaag acggcggtga cctgcacgcc gagctgggtc agggaagccg
     1441 cgacctcgca gccgacgaac cccatgccca ccaccaccgc gcgccgtccg gcctcggctt
     1501 cgcgcttgat ggcgtcgcac tccgcaaccg tgcgcaggta gtggatccct ggcagctcgg
     1561 ccccgggaag ctccagacgg cggttctgtc ctcccgtcgc gatcagcagc ttctcgtatc
     1621 ccagttcccg cccggacctc agggccagcg cgtgcgccgc aggatcgagt gcgaccacgg
     1681 agtcggggcg gaggtccacg tcgtgcgcct cgtaccagtc gggcggcctg acgtaccagt
     1741 cagtgagatc ctcttcggat cgtaggtagg tcttggacag tggggggcgg ccaaacggga
     1801 tccccggttc ccggctgacg agcacgactg gcccgttgaa cccctcgtcc cgcaaggtcg
     1861 cggcggcggt gcccccggcc ttgccacctc cgacgatcac gatcggcctt gtaccggttc
     1921 cctcagacat gcggcctcat cccctcggcc ttcgtcccga ggccccattt tgccccacga
     1981 aatggtgcgg tcggccggct cggtgagctt ggcctggtcc aaggagggtg cacgttgtga
     2041 tggtgcacgt tgtgatgggc agtggggaaa aatcggggag tcgttctcgc ttcccgttct
     2101 ctccacattt ccactgcgtc catctttgcc ttgatcgcct cgccgaccga tgggcccgat
     2161 ccgtcgacca ctgcggttct catgactgca gaaactgcag aaccgcgaga acccgacggt
     2221 tgtcgctctt ggacatcgac agcccgagct tggcgaagat gttgaccaca tgcttccagg
     2281 gggaaggcca cgtaaggtgg ccaccgatgc gatacatgat cctcgtccac ccctcgccgg
     2341 ggtcggagtc gggcgcgatg ccgacggagc agcagatcgc ggagcagctc gcgttccatc
     2401 aggcgatcga cgaggccggc atcctgatcg acgcgatggg cttccatccc acctcggacg
     2461 cgtggcgggt gcagcagcgg accgacgggt cccgcgaggt gatcgaggga ccgttccacg
     2521 acgagcacct gatcgccggc tacaccttga tcgacgtagc gtcgcgcgag gaggcgatgg
     2581 actggtcgct ccgctacgcg aagaccgcca ccgaggacga ggacggcgag atcgaggttc
     2641 gccggctgtt cgatctcgac gagttcgtcc agggcccggc cgtccgcggg ttccgggagc
     2701 tcgacctggt cggccggatc gccgccgacg tggggcggtc ccgacccgac ctgtcccgag
     2761 ccgtctcccc ggacggcacc gtgaccgtcc tgttcaccga catcgaggga tcgacgcagg
     2821 tcacagaggc gctcggcgac gccgagtgga tccgcgtgct ccgcgcccac aacgccctcg
     2881 tcagagatca ggtcgccgct cactccggca tcgaggtgaa gtcccagggt gacgggttca
     2941 tgctggcgtt cgcgtccgcg aaggacgccc tgctgtgcgc gatcggtatc cagcgggcgc
     3001 tcgctcaggc tccctcagat gggcatcggc tccgcgtgcg gatcgggctc cacacgggcg
     3061 agatgatccg cgaggaggac gacttcttcg ggaaagccgt ggtcctcgcc gcgcgaatcg
     3121 cagcggaggc acggggaggt gagatcctcg tgtcgcggct cgtccgagag ctgacggaag
     3181 gcgccagcct gttctcgttc accgatccca ccgacgtcga gctcaagggc ctctccggca
     3241 tgcactccct gtgggcggtt cgttggcaga ccgccatgag cggggaagcg ctcgcggcct
     3301 ctcagaggtg acgccgcgat gctcggtaca gcgtcaccgg cgtcgcgatt cgtgtacgcc
     3361 gcggggccaa tgcggggcca ctggctcatg tgaaacccgg atatggcctc tgacctgcgt
     3421 agtcccaacg gggttcgaac ccgtgtctcc accttgagag gttgagccgg ccggttccgt
     3481 gacctgcagc ggaggccgga atgcgccgtt cacctgtgga ttcggttccg ggtcggttcc
     3541 ggtcgttacc cctcgctttt cgatgtcatg cgggatagat gcgggatcaa agggcggcgc
     3601 agcgctaaaa ccaccttttc gtcagtctgg acgaaccggc cgcaagcggt actgaccgta
     3661 tcttgggggg agagagcggt ccttcgtagt cgttgccgtc gagtccgtag agggcgaagc
     3721 tcaccatgcg aacttcgttc aagacgaaca gcgccccccg ctggtgcggt gatccaattg
     3781 aggacgatgc cgcctgcagc gatgaccgcg aactgccgca ggcgaccatg aaggggagga
     3841 gcccgccatc ggcgggctcc tcccgtgaag cggcccggag ctcagaccgc tcgctctagc
     3901 tcgatgcccc cgcatactcc ggggtgctga cccggcgctc cacggacttg gcctccgccc
     3961 tgcccgcgtg ccggaacccg aggatcgaga agatcccgag gatcagtccg gcgatcacca
     4021 tcgcgagccc agcccacagg gcgatcgtcg ccagggtcca ccagccgtat gcgttcagca
     4081 gcatcgcccg tagcgactcg cccttgaaca gggtgtccgc cttcgcctgc aactccgctg
     4141 ccacgtccgg gtcgagtccc tctgcacgtg cggcggcgct cgtctccgag tacgtcgctc
     4201 cctcgttgac gccctcgagg tgcccggcga tg
//
LOCUS       DATBRK010000270         9292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2787717, whole genome shotgun sequence.
ACCESSION   DATBRK010000270 DATBRK010000000
VERSION     DATBRK010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2787717"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VIH70_08135"
     CDS             complement(<1..1239)
                     /locus_tag="VIH70_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEY8116367.1"
                     /translation="MATERLTALDASFLYLERPAMHMHVAGLSVLDPSTRPDGRLRFE
                     DVEQVFASRLHRAPRLRQKVKMVPFGLALPVWVDDAAFDLDFHIRRAALPHPGGRVEL
                     GEQVQRILSRPLDRSKPLWELYVIEGLEGGHVATLMKVHHAMIDGLSGMHLTAALFDL
                     SPEPPEEPPPPAWEPEPEPSSQDLLREATETLFAHPVQALMNGMDAVRRSPAVAALDL
                     GTVLSGFRSILDMGARPASPLDVQIGPNRRFAIADAPLQRFKDIKDTLGGTVNDAVLT
                     VVGGALHRLLRDRKEPTRGRTLRVMVPVSVQGGRDASLGNRVAPAFVDLPVGAMGPKR
                     RLALVREGTSHLKDSMMAMSADAIIGLGAYAPGGLLAAAARLASRGPWFNLVVSNIPG
                     PPQPMYMAGARLVAQYPSLPL"
     gene            1359..3287
                     /locus_tag="VIH70_08140"
     CDS             1359..3287
                     /locus_tag="VIH70_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY8116368.1"
                     /translation="MRRRLRAVTLLAATFALALPSMPARATSDGGYRAVEVRDNVFAP
                     RIVRVAVGGTIEWTNEGRSTHDVRADDGAFASSDLAPGDVFQETFPTAGAFPYFCSIH
                     GAPGVGMTGLVLVGDAPIPGAASDVGPGRETPPSLPGAEVRVPQDAPTIQAAVDAAEP
                     GGIVAISPGVYEEAVVVTTPFLTIRGLDRNRVILEGGFTLDNGIQVIEADGVTIENMT
                     ARHYVVNGFLWSGVFGYRGSYLTAFNDGDYGLFAFDSRYGQFDHSYASGHPDSGFYIG
                     QCFPCHAVITDVLAENNAVGYSGTNAGGGLFVVNSEWRDNLAGIVPNTLDSERLAPQH
                     DALIAGNWVHDNNNAGAPAFDLEYPSLGIGIIVNGGRGNVVTQNLVEGHEAFGIALLP
                     SPDEHLWLTQGNEVRDNLVRNSGDADLALGAPSAGGDCFSGNDFSSSLPPAIEWRAGC
                     GSPFARIPGGSVGAVFGPLERYLDAALGDVAVGDWRSQPPPPDQEQMPGAERAPPDPA
                     IAETAVPEPVRIRDARTLDVPASPDVSQEVTVLGIWLATSWWGVLIGLYAYVLPLILY
                     TAWVSIALWDLVRQEAVPNRTRVLWMLVVLVIPLAGPIGYFAFGRSPIQRSLRVMLVA
                     GGLAIYVALTALAIGLGP"
     gene            3438..3797
                     /locus_tag="VIH70_08145"
     CDS             3438..3797
                     /locus_tag="VIH70_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8116369.1"
                     /translation="MKYLIMMFGDQGTMIETRSTEWIRSMIAFMHDLDHELRDAGEYV
                     DGQGLVDPTQAKTVRFESGAPVVTDGPFAEAKESLAGYWIVEVSEERALEIASRIVTF
                     IEGPIEVRQIADAPPEY"
     gene            3842..4339
                     /locus_tag="VIH70_08150"
     CDS             3842..4339
                     /locus_tag="VIH70_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116370.1"
                     /translation="MPKITPFLWFDDQAEEAAELYVSVFNARPGSSGDSEIRGVSRYG
                     EAGPGKPGSAMTVSFRLEGQDFTGLNGGPHHAFSEAVSFHVSCETQDEVDALWSALSD
                     GGEEGPCGWLKDRYDLSWQIIPTALGELLGDPDPERGARAMQAMLQMTKIDIQGLRRA
                     AEAAA"
     gene            4348..5571
                     /locus_tag="VIH70_08155"
     CDS             4348..5571
                     /locus_tag="VIH70_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEY8116371.1"
                     /translation="MSSVDAPGVEDLLRELAPQVLGVLVRRYGRFDASEDAVQDALLT
                     AAMQWPDGGVPENPRAWLITVASRRLIDGWRSESARRQREERVTVLDVPEPGPVAGRD
                     DTLTLLFLCCHPSLSVPSQLALTLRAVGGLTTAEIANAFLVPEPTMAQRISRAKQTIR
                     NAGARFDLPPEAEREDRLRVVLHVLYLLFNEGYATSSGPALHRADLTSEAIRLTRLLR
                     GLLPRHGEVAGLLALMLLTDARRGARTTPDGALVPLAEQDRALWDGTQIEEGVGIIGD
                     TLGRAAIGPYQLQAAIAAIHDEAPNADETDWPQILALYDVLEQVSPGPVVTLNRAVAV
                     AMVDGPRAGLAVLGTLDADDRMTHTHRLDAVRGHLLELAGEPGAARESYLRAARMTAS
                     VPEQRYLAMRAARLR"
     gene            complement(5568..6482)
                     /locus_tag="VIH70_08160"
     CDS             complement(5568..6482)
                     /locus_tag="VIH70_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8116372.1"
                     /translation="MSATDPPARHDLLGGASAAVSAVLFGTIIIWGRFVLERGVPVET
                     MLSIRFAIGAVALLLALLLTRRPVMAVRGERLRLVLLAVFGYAIEASLFFTATQHGTV
                     AAVTLLFFTYPVFVTVWAWALGGDLPARLTLFALVCAVAGAGIVVGTGTGLAIDGVGV
                     LLAFVTAMTYSAYLTGVDLLVGRTNALTSAMWISAGASIGLFVYANVVGHWDLPAGAA
                     EWWPLVGMGLSSAGAFFFLMEALARIGAVRTSIVSAMEPLAAAVLGFVFLDESVTFGV
                     AAGGLLILAGAVTASLARTPTPQEQQIP"
     gene            complement(6479..7186)
                     /locus_tag="VIH70_08165"
     CDS             complement(6479..7186)
                     /locus_tag="VIH70_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEY8116373.1"
                     /translation="MTEELFATDAYVRSCKAAVKEAGDEGVVLDRTVFYARSGGQPGD
                     TGVLRWEGREVRVLDTLKRGGRLLHEVDGDPPAPGIDVTAEIDWDRRHTLMRTHTALH
                     ALSAVVFRDYDAKVTGGNMEPGIARMDFELESIDAEFGREVEEKLNLELAADRPVLVG
                     FLPRALALADPDLIRTKVNLIPESVDPIRVIEIEGLDKQADGGTHVRSTAEVGHVRVA
                     KTENKGKAFKRMRIELA"
     gene            7289..8719
                     /locus_tag="VIH70_08170"
     CDS             7289..8719
                     /locus_tag="VIH70_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8116374.1"
                     /translation="MSRQPHIVARFAVASLAAFVLVGAVGMVLMIRYARERAERAGAF
                     HAAFVATSVLAPALSGLDLSRPITGDDAARVRELVAERILADGRDVRVKVWRLDGTIV
                     FSDAPNLVGKHFPEEAAELAEVVAGHPEIGISDLDDPENVAERGLADKLLFAYVPLRI
                     EPGGPVVAVAELYQDYAAIQEDIDAFVARMLPLLGIGLLALYAAILPIAARVSTELRR
                     RNERLAELLEREQQTVAELRDLAKKKDDFVGAASHELRTPLTSIRGSLATLKGAGLGA
                     DEATRIELLDAAERQTKRLERLVVNILATARLDGDRPIEIEPVDLAETARAVAKELGA
                     SDRVVVVVPEGNVETDRSRIVDVMTYLLENALKYSPDGSTVTIGADSERSGFRLWVTD
                     HGVGIDPADQAAIFERFHQIDQSSTRSHGGLGLGLHLAKELVEELRGRVDVASTPGRG
                     STFTVSVPSHARAGASSRSPLSTPAG"
     gene            complement(8783..>9292)
                     /locus_tag="VIH70_08175"
     CDS             complement(8783..>9292)
                     /locus_tag="VIH70_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116375.1"
                     /translation="AGAIGNLVCREPERFERMVWLLPAGLDLPFGLANRYHLLAEDLE
                     GKTPQEALETVLGDPARVAEYVETPWRLELDRVLWQHDDPDGLARAIHGVVEDWPIPN
                     RELLRDVATPTIIVTIEGDEIHPAELGLLLSELLPNAELIALGSQEELLRRIPELVAR
                     VSAFIAGNG"
BASE COUNT         1360 a         3351 c         3259 g         1322 t
ORIGIN      
        1 gagcgggagc gaggggtact gcgcgaccag ccgcgcgccc gccatgtaca tcggttgcgg
       61 cggcccgggg atgttcgaaa cgacgaggtt gaaccaggga ccccggctcg ccaggcgggc
      121 ggcggcggcg agcaggccgc ccggcgcgta ggcgccgagg ccgatgatcg cgtccgcgct
      181 catcgccatc atcgagtcct tcaggtggct ggtcccctcg cgcacgagcg cgagtcgacg
      241 cttgggcccc atcgcgccga ccggcaggtc gacgaatgcc ggcgccaccc ggttgccgag
      301 cgacgcgtcg cggcctccct gcaccgagac cggcaccatc acgcggagcg tgcggccccg
      361 ggtcggttcc ttccggtccc gcaacagccg atgcagcgcg ccgcccacga cggtgagcac
      421 cgcgtcgttc accgtcccgc ccagcgtgtc cttgatgtcc ttgaagcgct ggagcggcgc
      481 atcggcgatg gcgaaccgcc ggttggggcc gatctgcacg tcgagcggcg acgcgggccg
      541 cgcgcccatg tccaggatgc tgcggaaccc cgacaggacg gtgccgaggt cgagcgccgc
      601 gaccgcgggc gagcgccgga ccgcgtccat cccgttcatc agcgcctgga ccgggtgggc
      661 gaacagggtc tcggtggcct cgcggagcag gtcctgcgag gacggctcgg gttcgggctc
      721 ccaggcgggc ggcggtggtt cctcgggcgg ctccggcgac agatcgaaca gggccgcggt
      781 cagatgcatg cccgagagcc cgtcgatcat cgcgtgatgc accttcatca gggtcgcgac
      841 gtgcccgccc tcgagcccct cgatcacgta gagctcccac agcggcttcg atcggtcgag
      901 cgggcgagag aggatcctct ggacctgttc gccgagctcg acccggccgc cggggtgcgg
      961 gagcgccgcg cgtcggatgt ggaagtccag gtcgaacgcg gcatcgtcca cccagaccgg
     1021 caacgcgagc ccgaacggga ccatcttcac cttctggcgg aggcgcggcg cgcggtgcag
     1081 gcgcgacgcg aacacctgct ccacgtcctc gaaccgtagc cgtccgtccg gacgcgtcga
     1141 cggatcgagc acggagagcc cggcgacgtg catgtgcatc gccggccgct ccaggtacag
     1201 gaacgacgcg tcgagcgcgg tcaggcgttc ggtggccatc ggaccctttc ccggggggct
     1261 gtcgctcgcg cggcagcgct cgctcctcga gtcccccgca cccccatcac cgtaccgctc
     1321 cacgaccggc gtcgaaaccg gtgctagcct cgcccgcgat gcgccgacgc ctgcgcgccg
     1381 tcaccctgct cgccgccacg ttcgcgctcg ccctcccctc gatgccggcc cgggccacga
     1441 gcgacggggg gtaccgagcc gtcgaggtgc gggacaacgt cttcgcccca cggatcgttc
     1501 gggtcgcggt gggtgggacg atcgagtgga cgaacgaggg acggagcacg cacgacgtca
     1561 gggccgacga cggagcgttc gcctcgtccg atctcgcgcc cggcgacgtg ttccaggaga
     1621 cgttccccac cgccggggcc ttcccgtact tctgctcgat ccacggggcg ccgggcgtcg
     1681 ggatgaccgg cctggtcctg gtcggcgacg caccgatccc gggtgccgca tccgacgtcg
     1741 gacccggtcg cgagacgccg ccctcgctcc cgggggccga ggtccgtgtc ccccaggacg
     1801 cgccgacgat ccaagccgcg gtcgacgcgg ccgagcccgg cggcatcgtc gccatctcgc
     1861 ccggcgtcta cgaggaggcg gttgtcgtga cgacgccgtt cctgacgatc cgtgggctcg
     1921 accgcaacag ggtgatcctg gagggggggt tcacgctcga caacgggatc caggtcatcg
     1981 aggcggacgg cgtcacgatc gagaacatga ccgcccgcca ctacgtggtg aacgggttcc
     2041 tgtggtccgg cgtgttcggc taccgcgggt cgtacctgac ggcgttcaac gacggggact
     2101 acgggctgtt cgcgttcgat tcccggtacg ggcagttcga ccactcgtac gcgagcgggc
     2161 acccggactc cggcttctac atcggccagt gcttcccatg ccacgccgtg atcaccgacg
     2221 tactcgccga gaacaacgcc gtggggtact cgggcacgaa cgccggcggc ggcctgttcg
     2281 tcgtgaactc cgagtggcgc gacaacctcg cgggcatcgt gccgaacacc ctggactccg
     2341 aacggctcgc accgcagcac gacgccctga tcgccggcaa ctgggtgcac gacaacaaca
     2401 acgccggcgc gcccgcgttc gacctggagt atccgtcgct cgggatcggg atcatcgtga
     2461 acggcggacg cggcaacgtc gtcacgcaga acctcgtcga gggccacgag gccttcggga
     2521 tcgcgctcct cccctcgccc gacgaacacc tctggctcac gcagggcaac gaggttcgcg
     2581 acaacctggt tcggaactcc ggtgacgccg atctcgcgct cggcgctccc tcggcgggcg
     2641 gcgactgctt ctcgggcaac gacttctcct cgagcctgcc gccggcgatc gaatggcggg
     2701 ccggctgcgg gtccccgttc gcccggatcc ccggcggctc ggtcggcgcc gtgttcggcc
     2761 cgctcgagcg ctacctcgac gcggcgctcg gggacgtcgc cgtgggcgac tggcggtcgc
     2821 agccgccgcc acctgatcag gagcagatgc cgggcgccga gcgcgccccg cccgacccgg
     2881 cgatcgccga gaccgccgtc cccgaacccg tccgcatccg cgacgcccgc acgctcgacg
     2941 tgccggcctc gcccgacgtg tcccaggagg tcaccgtgct cgggatctgg ctcgccacga
     3001 gttggtgggg cgtgctcatc ggcctgtacg cgtacgtgct gccgctgatc ctgtacacgg
     3061 cgtgggtctc gatcgcgctg tgggacctgg tccgccagga ggccgtcccg aaccggaccc
     3121 gtgtcctgtg gatgctggtc gtgctcgtga tcccgctcgc cgggccgatc gggtacttcg
     3181 cgttcgggcg ctcgccgatc cagcggtcgc tgcgggtgat gctggtggcc ggcgggctcg
     3241 cgatctacgt cgccctgacc gcgctcgcga tcgggctcgg gccctagggc cggcacgtct
     3301 cggctcgctc cttgcgtgag cgccccccgc gccgccggcc cggtcgccta gggtttcccg
     3361 ggcggatgtc gagatccgct cggccgctcc gaccaactcg tgagcgcgcc acccgggcgc
     3421 cgaggaggga gtggaggatg aagtacctca tcatgatgtt cggggaccag ggcacgatga
     3481 tcgagactcg gtccaccgag tggatccggt cgatgatcgc gttcatgcat gacctcgacc
     3541 acgagctccg ggatgccggc gagtacgtcg acgggcaggg gctcgtcgac ccgacccagg
     3601 ccaagaccgt ccgcttcgag agcggtgccc cggtcgtgac cgacggaccc ttcgcggagg
     3661 ccaaggagtc gctggccggc tactggatcg tcgaggtctc cgaggagcgg gcgctcgaga
     3721 tcgcgtctcg gatcgtcacg ttcatcgagg gcccgatcga ggtacggcag atcgccgacg
     3781 cgccgcccga gtactgagtc cgagcacgcc gaccgcgcac gacaacaaga ggaggaccga
     3841 gatgccgaag atcacgccgt tcctgtggtt cgacgaccag gccgaggagg ccgccgagct
     3901 ctacgtgtcc gtcttcaacg cgaggccggg ctcctccggc gactcggaga tccggggagt
     3961 ctcgcgctac ggcgaggccg gtccgggcaa gccgggctcg gcgatgaccg tgtcgttccg
     4021 gctcgagggg caggacttca ccggactgaa cggtggcccg caccacgcgt tctccgaggc
     4081 ggtgtcgttc cacgtgagct gcgagacgca ggacgaggtc gacgccctct ggtcggcgct
     4141 ctccgacggt ggcgaggagg gaccctgcgg gtggctgaag gaccggtacg acctgtcgtg
     4201 gcagatcatc cccaccgcgc tgggcgagct cctcggcgac ccggaccccg agcggggcgc
     4261 gagggccatg caggcgatgc tgcagatgac caagatcgac atccaggggc tccgccgcgc
     4321 cgcggaggcc gcggcgtaac gacgccgatg tcatcggtcg acgcgcccgg cgtcgaggac
     4381 ctgctgcgcg agctggcgcc gcaggtcctc ggcgtgctcg tccgccggta cggtcggttc
     4441 gacgcgagcg aggacgcggt gcaggacgcg ctgctcaccg ccgcgatgca atggccggat
     4501 gggggcgtcc ccgagaaccc tcgcgcctgg ctgatcacgg tggcctcccg caggctgatc
     4561 gacggatggc gcagcgagag cgcccgccgt cagcgcgagg aacgggtcac ggtcctcgac
     4621 gtgcccgagc cgggaccggt cgccgggcgg gacgacacgc tgaccctgct gttcctgtgc
     4681 tgtcacccgt cgctctcggt gccgtcgcag cttgcgctga cgctccgggc ggtcggcggc
     4741 ctgaccaccg ccgagatcgc aaacgccttc ctggtccccg agcccacgat ggcccagcgc
     4801 atcagccggg ccaagcagac gatcaggaac gccggtgccc gattcgatct gccgcccgag
     4861 gcggagcgcg aggatcggct ccgcgtcgtc ctgcacgtgc tctacctcct gttcaacgag
     4921 ggctacgcga cgagctcggg tcccgcgctg caccgggccg acctgacctc cgaagcgatc
     4981 cggctcacgc ggctgctccg agggctcctg ccccggcacg gcgaggtcgc cggcttgctg
     5041 gcgctgatgc tgctgaccga cgcccggcgc ggggcgagga ccaccccgga cggcgccctg
     5101 gttcccctgg ccgaacagga ccgcgccctg tgggacggga cgcagatcga ggagggcgtc
     5161 gggatcatcg gcgacaccct ggggagggcg gccatcgggc cgtatcagct ccaggcggcg
     5221 atcgcggcga tccacgacga ggcgccgaac gccgacgaga ccgactggcc ccagatcctc
     5281 gccctgtacg acgtgctcga acaggtctcg cccggccccg tcgtgacgct caatcgggcg
     5341 gtcgccgtcg cgatggtcga cggcccacga gcgggtctcg cggtgctcgg cacgctggac
     5401 gccgacgatc gcatgaccca cacgcaccgg ctcgacgcgg ttcgggggca tctgctcgag
     5461 ctcgcgggcg agcccggcgc ggcccgggag tcctacctgc gtgcggcgag gatgaccgcc
     5521 agcgtccccg aacagcgcta cctcgcgatg cgcgccgccc ggctgcgcta ggggatctgc
     5581 tgctcctgcg gggtgggcgt gcgggcgagg ctcgccgtca cggcgccggc aaggatcagg
     5641 aggccacccg cggccacccc gaaggtgacc gactcgtcca ggaacacgaa cccgaggacc
     5701 gcggccgcga gcggctccat cgccgagacg atggacgtgc ggacagcgcc gatcctggcg
     5761 agcgcctcca tcaggaagaa gaaggccccg gcggacgaca ggcccatgcc gacgagcggc
     5821 caccactcgg cggcaccggc gggcaggtcc caatggccga cgacgttggc gtagacgaac
     5881 agcccgatcg acgcacccgc gctgatccac atcgcgctgg tgagcgcgtt ggtgcgaccg
     5941 accagcaggt ccacgcccgt gaggtaggcg ctgtacgtca tcgcggtcac gaacgcgagg
     6001 agcacgccga cgccgtcgat cgcgaggccg gtccccgtac cgacgacgat tcccgctccg
     6061 gcgaccgcgc acacgagggc gaacagcgtg aggcgcgccg gcagatcgcc gcccagggcc
     6121 cacgcccaga ccgtcacgaa gacgggatag gtgaagaaca ggagcgtcac ggcggcgacc
     6181 gtcccgtgct gggtggccgt gaagaacagg ctcgcctcga tcgcatagcc gaacaccgcg
     6241 agcaggacca gccggagccg ctcgccgcgc accgccatca ccggccgacg agtcagcagc
     6301 agcgcgagca acagcgcgac cgcgccgatc gcgaaccgga tcgacagcat cgtttcgacc
     6361 gggacgcctc gctcgagcac gaatcgaccc cagatgatga tggtgccgaa cagaacggcc
     6421 gagacggcgg cggaggctcc cccgagcagg tcgtgacgcg cgggcggatc ggtcgcgctc
     6481 aggcgagctc gatccgcatc cgcttgaagg ccttgccctt gttctcggtc ttcgcgacgc
     6541 ggacgtgccc gacctcagcc gtagaacgca cgtgggtccc gccgtccgcc tgcttgtcga
     6601 gcccctcgat ctcgatcacg cggatcgggt ccaccgactc ggggatcagg ttcaccttcg
     6661 tacggatcag gtcgggatcg gcgagggcaa gggcgcgcgg caggaagccc acgaggaccg
     6721 ggcggtcggc cgcgagctcc aagttcagct tctcctcgac ctcccgaccg aactccgcgt
     6781 cgatggactc gagctcgaag tccatccgcg cgatgccggg ttccatgttc ccgccggtga
     6841 ccttcgcgtc gtagtcgcgg aacaccaccg ccgacagcgc gtgcagtgcg gtgtgcgtgc
     6901 gcatgagcgt gtggcggcga tcccagtcga tctcggccgt gacgtcgatc cccggggcgg
     6961 gcggatcgcc gtcgacctcg tgcagcagcc tgccgccgcg cttgagggtg tcgagcactc
     7021 ggacctcccg gccctcccac cgcagcaccc cggtgtcccc cggctgcccg ccgctgcggg
     7081 cgtagaagac ggtcctgtcc aggacgacac cctcgtcgcc ggcctccttc acggcggcct
     7141 tgcacgaccg gacgtaggcg tcggtggcga agagctcctc ggtcatgccg ggcagcctag
     7201 ccgacgtggc cgccgggcct ccccgcagcc gctcgccggg aggatgactc tcaggcgccg
     7261 tcgggttcgt tccgatacag gacctgagat gtcaagacaa ccgcacatcg tcgcccggtt
     7321 cgccgtcgcg agcctggcgg cgttcgtgct cgtcggggcg gtcgggatgg tcctgatgat
     7381 ccggtacgcg cgcgagcgag cggagcgggc cggggccttt cacgcggcct tcgtcgccac
     7441 gtcggtcctg gctcccgcgc tctccggtct cgatctgtcc cgtccgatca ccggcgacga
     7501 tgcagctcgc gttcgcgagc tggtcgccga gcgaatcctg gccgacggcc gcgacgtccg
     7561 cgtcaaggtc tggcggctcg acggcacgat cgtgttctcc gacgctccca atctcgtcgg
     7621 gaagcacttc ccggaggaag cggcggagct ggccgaggtc gtggccggtc accccgagat
     7681 cggcatctcg gatctcgacg accccgagaa cgtcgccgag cgcggcctgg ccgacaagct
     7741 gctgttcgcc tacgtgccgc tgcggatcga gcccggcggc ccggtcgtcg ccgtcgcgga
     7801 gctctaccag gactacgcgg ccatccagga ggacatcgac gccttcgtcg ccaggatgct
     7861 tccgctgctt gggatcggcc tgctcgcctt gtacgcggcg atcttgccga tcgccgcgcg
     7921 ggtctcgacc gagctcaggc ggcggaacga acggctggcg gagctcctgg agcgcgaaca
     7981 gcagaccgtg gcggagctcc gtgacctggc caagaagaag gacgacttcg tgggcgccgc
     8041 gtcgcacgag ctccggacgc cgctcacatc gatccgcggc tcgctcgcga ccctcaaggg
     8101 ggcgggtctc ggggccgacg aggcgacgcg catcgaactc ctcgacgctg ccgagcgtca
     8161 gaccaagcga ctggagcgcc tcgtcgtcaa catcctggcg accgctcgcc tggacggcga
     8221 tcgcccgatc gagatcgagc cggtcgatct cgccgagacc gctcgtgcgg tcgccaagga
     8281 gctcggcgcc tcggaccggg tcgtcgtcgt ggttcccgag gggaacgtgg agaccgaccg
     8341 ctcccgtatc gtcgacgtca tgacgtacct gctcgagaac gccctgaagt actcgcccga
     8401 cggctcgacc gtcacgatcg gcgccgacag cgaacgatcg gggttccgtc tttgggtcac
     8461 cgaccacggc gtcggcatcg atccggccga tcaggccgcg atcttcgagc gcttccatca
     8521 gatcgaccag agctccaccc gatcgcacgg tggcctcggg ctcggtctgc acctggcgaa
     8581 ggagctggtc gaggagctcc gggggcgggt cgacgtcgcg agcaccccgg gtcgcggcag
     8641 cacgttcacg gtctcggtcc cgtcccacgc aagggcgggc gcctcgagtc gttctcccct
     8701 gtccacccca gcgggctgaa tcgcctcggc cctcgaacgt cttctcaccg gcgcggatgg
     8761 ggcaccgagc gccggtttcg aactatccgt tccccgcgat gaacgcggag acgcgcgcga
     8821 cgagctcggg gatccgccgg agcagctcct cctggctccc gagcgcgatc agctcggcgt
     8881 tcggcagcag ctcggacagg agcaggccga gctccgccgg gtggatctcg tcgccctcga
     8941 tcgtcacgat gatcgtcggc gtcgcgacgt cccggagcag ctcccggttc gggatcggcc
     9001 agtcctcgac cacaccgtgg atcgcgcggg cgagcccgtc tggatcgtcg tgctgccaca
     9061 gcacgcggtc gagctccagg cgccacggcg tctcgacgta ttcggccacg cgggccggat
     9121 cgcccagcac ggtctcgagc gcctcctgcg gcgtcttgcc ctccaggtcc tcggcgagca
     9181 ggtggtagcg attcgccagc ccgaacggca ggtccagtcc ggccggcagc agccagacca
     9241 tccgttcgaa ccgttcgggt tcgcggcaca cgaggttccc gatcgcgccc gc
//
LOCUS       DATBRK010000271         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1489632, whole genome shotgun sequence.
ACCESSION   DATBRK010000271 DATBRK010000000
VERSION     DATBRK010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489632"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VIH70_08180"
     CDS             <1..270
                     /locus_tag="VIH70_08180"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEY8116376.1"
                     /translation="TLGRDASALQSSLDVVLSRFAEYQGLEGAFARRAITERAKGILM
                     ERHAIDEKRAFEMLRDEARRSNHKIIDVAEAVAMSVNLLPRSQAD"
     gene            325..741
                     /locus_tag="VIH70_08185"
     CDS             325..741
                     /locus_tag="VIH70_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019667966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY8116377.1"
                     /translation="MPEPNDWNAGIIEEFRANGGRVGGYFDGATLLILHAKGIRSGLS
                     REKPLVYLPDGERFVVFGTMGGAPTDPMWVRNVLADPEPSVELGDRTVPVRAVEITGP
                     EADELYARQVERRPGFAEYKKKTSRWIPVIALEPTT"
     gene            1022..1420
                     /locus_tag="VIH70_08190"
     CDS             1022..1420
                     /locus_tag="VIH70_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116378.1"
                     /translation="MGERPRRHDEIRGATVLLTGWSRGREREIADALGAISTEPVDLE
                     DIELPFVALPKVTLEKAEAARGLLIEAGGSVDLRDEWVTRDAPRPSAARPTCPFCGST
                     KTQPYTHAGPAARTSMKCTTCGQRFRVSSR"
     gene            complement(1429..>1692)
                     /locus_tag="VIH70_08195"
     CDS             complement(1429..>1692)
                     /locus_tag="VIH70_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY8116379.1"
                     /translation="VLERLSRRLVDGVLTVRASEHRSQVRNREAALERMARILADALA
                     PPPPRRRPTRPTRGAVERRLADKKRRSRTKRLRLRDEGRSDED"
BASE COUNT          268 a          581 c          612 g          231 t
ORIGIN      
        1 acgctcggcc gggacgcgtc ggcgctgcag agctcgctcg acgtcgtgct gtcgcggttc
       61 gccgagtacc agggtctcga gggcgcgttc gcccgccgcg cgatcaccga gcgagccaag
      121 ggcatcctga tggagcggca cgcgatcgat gagaagcgcg cgttcgagat gttgcgtgac
      181 gaggcccggc gctccaacca caagatcatc gacgtcgccg aggcggtcgc gatgagcgtc
      241 aacctcctgc cacgctcgca ggccgactag gatcggccaa ccactcccga cgccgtaccc
      301 ttggtggccg agcacggagg acgcatgcca gaaccgaacg actggaacgc cgggatcatc
      361 gaggagttcc gcgcgaacgg cggcagggtc ggcgggtact tcgacggtgc cacgctgctg
      421 atcctccacg ccaagggcat caggagcggc ctctcgcgcg agaaaccgct ggtgtacctg
      481 cccgacggcg agcggttcgt ggtgttcggg accatgggcg gggcaccgac cgatccgatg
      541 tgggtgcgca acgtgctcgc ggaccccgag ccctcggtgg agctcggcga tcgaacggtg
      601 cccgtgcggg cggtcgagat caccggcccc gaggccgacg agctctacgc gcggcaggtg
      661 gagcggcgac ccgggttcgc cgagtataag aagaagacct cccgttggat cccggtcatc
      721 gcgctcgaac ccacgacctg acccgttcgg tcaagccgag cggtcatcgc gccgaagcat
      781 cacatgtgag atccgacggc cagaccgccc ggacgaagct cggcgatgcg ctgggccgcc
      841 ggagggcgcg acgcggcgca gacacgagct accgagatgc cctcgtcgcg cgtctggtca
      901 tgaacctcgc cgatctccgg ctgaggaggc gagcccagga gcgtgcccgc cggcaggcct
      961 ccagctccgt ctaggcgccg actacactgc cggcgccgtt cgggcgcgaa ggaggcagcc
     1021 ggtgggtgag cgaccgcgcc ggcacgacga gatcaggggt gcgacggtcc tgctaaccgg
     1081 gtggtcacgg ggccgcgagc gcgagatcgc cgacgcgctc ggcgcgatct cgacggagcc
     1141 ggtggatctg gaggacatcg agcttccctt cgtcgcgctc ccgaaggtca cgctcgagaa
     1201 ggcggaggcc gcacggggcc tcctgatcga ggcgggcggc tcggtcgacc tgcgagacga
     1261 atgggtcacg cgcgatgccc ctcgaccgag cgccgcgcga ccgacgtgcc cgttctgcgg
     1321 ttcgacgaag acgcagccgt atacgcatgc cggacccgcg gcccgcacgt cgatgaagtg
     1381 caccacgtgc ggtcagcggt tccgcgtctc gagccgctga cgacggtctc agtcctcatc
     1441 actgcgcccc tcatcacgga gccggagtcg cttcgtccgc gaacgacgct tcttgtcggc
     1501 cagacggcgt tcgaccgcgc cgcgcgtcgg cctggtcggg cgccgtcgag gcggcggcgg
     1561 cgcgagcgcg tcggcgagga tcctcgccat gcgttcgagc gcggcctcgc ggttgcggac
     1621 ctgcgagcgg tgttcggacg ccctcacggt caggacgccg tcgacgagcc ggcgcgagag
     1681 ccgttcgagc ac
//
LOCUS       DATBRK010000272         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_764821, whole genome shotgun sequence.
ACCESSION   DATBRK010000272 DATBRK010000000
VERSION     DATBRK010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764821"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1757
                     /locus_tag="VIH70_08200"
     CDS             120..1757
                     /locus_tag="VIH70_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8116380.1"
                     /translation="MAAVGEAPTLFLRKATGLVKGWSKFDAFLYSFMSVNFVTLGLFF
                     ALSVLAFVPTGQVLPALLISGVFVTFLVITYAGLISVMPRAGGDYVWQSRVMGGGIAF
                     VLAVTGWWFILWYWAPIYGNILNVELLQPLAATFKWDGLTDWLATDTGLFVVILGTVV
                     LAGYLVSLGMEGYAKVQKVCFYIGIIALAVVFLVMLFGSRSGFQSAFNQETANLFNAK
                     GDVYQQTIQLGTTNNGGPIVPNLGFSPFFGDTLLLIPFLCFWILWPNWGATLYGEVRG
                     ASDFKRVMGGMMYGLWVTVAVAVVFILLADKFFGWEFFNAANLNYWATVYGFGDASIP
                     VWSYAPFLASFYFHSSLITALIVLAFGAWFLGWAGTLFLSSTRVIFAAAFDRVLPERV
                     ADVSEKRHVPVWALVLMLVPSVAVGALYAYTSKFSTYILDATLVIAVTFFGTAIAAMI
                     LPYRKKSLYANSPIARYKILGLPLITVTGFVTAAFLGFNLWHWFTNDLYAVNNSESLW
                     FMGIMYALALVIYVVAKVYRKRQGIDLSAVYREIPVE"
     gene            1834..>2241
                     /locus_tag="VIH70_08205"
     CDS             1834..>2241
                     /locus_tag="VIH70_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8116381.1"
                     /translation="MADEHRMFIGGEWVGSGSGATFEATSPSTGEVIGTVPEGTRDDV
                     RRAVAAADEAFGTWSGLTAFDRARAMRRVAEAIDGRRDDLARTLALDQGKPMRAEAFD
                     EVEELIAYFEMAAADAVRVEGLIPPSVTQGNRVL"
BASE COUNT          326 a          687 c          788 g          440 t
ORIGIN      
        1 tacgaggagg ggatcctcca ggcggccgtc gtgacgatcg acgcgaagaa gcggaaggtg
       61 aagaactacc aactggtgaa cggctagatc gccgttccgt acctcgggag ggggtccaga
      121 tggcagcggt aggcgaagct cctaccttgt tcctgcgcaa ggcgaccggc ctcgtgaagg
      181 ggtggtcgaa gttcgacgcg ttcctctact ccttcatgtc ggtcaacttc gtgacgcttg
      241 ggttgttctt cgcgctgtcg gtgttggcgt tcgtgccgac cgggcaggtg ctcccggccc
      301 ttctgatctc gggcgtgttc gtcacgttcc tggtcatcac gtacgcgggg ctgatctcgg
      361 tgatgccccg cgccggtggg gactacgtgt ggcagagccg cgtcatgggc ggcgggatcg
      421 cgttcgtcct tgcggtcacc gggtggtggt tcatcctgtg gtactgggcg ccgatctacg
      481 ggaacatcct caatgtcgag ctgttacagc cgctcgcggc cacgttcaag tgggacggct
      541 tgacggactg gttggcgacc gacaccgggc tgttcgtcgt gatcctgggg acggtggtgc
      601 tggccggcta cctggtctcg ctggggatgg aggggtacgc gaaggtccag aaggtctgtt
      661 tctacatcgg catcatcgcc cttgccgtcg tgttcctcgt gatgctgttc gggtcccgca
      721 gcggtttcca gagcgcgttc aaccaggaga cggccaacct gttcaacgcc aagggcgacg
      781 tctaccagca gacgatccag ctcgggacga cgaacaacgg cgggccgatc gtgccgaacc
      841 tcggtttctc gccgttcttc ggcgacacgc tgctgctgat cccgttcctg tgcttctgga
      901 tcctgtggcc gaactggggc gccacgctgt acggcgaggt ccgcggcgcg agcgacttca
      961 agcgcgtgat gggcgggatg atgtatggcc tctgggtcac ggtcgcggtc gcggtcgtgt
     1021 tcatcctgct cgcggacaag ttcttcgggt gggagttctt caacgcggcg aacctcaact
     1081 actgggccac ggtctacgga ttcggggacg cgtcgatacc ggtctggtcc tacgcgccgt
     1141 tcctggccag cttctacttc cacagttcgc tgatcaccgc gctcatcgtg ctcgcgttcg
     1201 gcgcgtggtt cctcgggtgg gcgggcacgc tgttcctctc gtcgacccgc gtgatcttcg
     1261 cggcggcgtt cgaccgcgtg ctgcccgaga gggtggccga cgtctcggag aagcgacacg
     1321 tgccggtgtg ggcgctggtg ctgatgctcg tgccctctgt cgcggtcggg gcgttgtacg
     1381 cgtacaccag caagttctcc acgtacatcc tggatgcgac gctggtgatc gcggtaacgt
     1441 tcttcggcac ggcgatcgcg gccatgatcc tgccctaccg caagaagtcg ctgtacgcga
     1501 actcgccgat cgcccggtac aagatcctcg ggctgccgtt gatcacggtg accgggttcg
     1561 tgaccgctgc cttcctgggg ttcaacctgt ggcactggtt cacgaacgac ctgtacgcgg
     1621 tcaacaacag cgagtccttg tggttcatgg gcatcatgta cgcgctcgcg cttgtgatct
     1681 acgtggtggc gaaggtctac cgtaagcgcc aagggatcga cctgagcgcc gtctacaggg
     1741 agatcccggt cgagtaggag ccggagcacg aggcggatct ggggcggccc tcgcggccgc
     1801 cccaggccgt cacgggaccg tcgggacggg atgatggcgg acgaacatcg gatgttcatc
     1861 ggcggcgagt gggtcggctc ggggagcggg gcgacgttcg aggcgacctc gccctccacc
     1921 ggcgaggtga tcggcacggt acccgagggc acccgtgacg acgttcgccg cgccgtcgcc
     1981 gcggccgacg aagcattcgg gacgtggtcg gggctcacgg cgttcgaccg cgccagggcg
     2041 atgcgccgcg tggccgaggc gatcgacggc cgccgcgacg acctcgcgcg tacgctcgca
     2101 ctcgaccagg gcaagccgat gcgggcggag gcgttcgacg aggtcgagga gctgatcgcg
     2161 tacttcgaga tggcggcggc cgacgccgtc cgggtcgagg gcctcatccc gccctcggtg
     2221 acccagggca accgtgtgct c
//
LOCUS       DATBRK010000273         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_329334, whole genome shotgun sequence.
ACCESSION   DATBRK010000273 DATBRK010000000
VERSION     DATBRK010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329334"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VIH70_08210"
     CDS             <1..706
                     /locus_tag="VIH70_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116382.1"
                     /translation="PGPERDHRYGSVAMQITPRDKRILQIAGVVVPLLLLVYFFVLRP
                     DGDEDLALPNGPTGITGSTGAPTESPSVTPSPTPRETLPPVSLAGSRDPFSIPPGLET
                     STGGSVSPAATAPTGTTTTIPATTTFPPPPPPPTTTTTTFPPPPPPTTTTTPPPGGGG
                     GGGERPGNKILVGGHNVKLLGVAGGGRKLDVAVDGKVYTVQPGATFDDNFKLVKIDGK
                     CATFLFGDQSFELCEK"
     gene            949..>1704
                     /gene="aroC"
                     /locus_tag="VIH70_08215"
     CDS             949..>1704
                     /gene="aroC"
                     /locus_tag="VIH70_08215"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY8116383.1"
                     /translation="MLRLLTAGESHGKALLGVLEGLPAGIPVSPKEIATELARRRHGH
                     GRSGRQKLEGDRFEIVSGVRHGLTLGSPIGVTIENAEWDVKYRDLMSVEGEADPAERL
                     TRPRPGHADLAGAMKYGFDDVRNVLERASARETAMRVALGVFCKALLAELGIAVVSHV
                     VRIGKVAVRSTRVPRPEDLETIDASPVRCLDEVVADRMVAEIDRARKARDTVGGVFEV
                     IAFGCPPGLGSHVQYDRKLDARLALSLMSIQSVK"
BASE COUNT          258 a          605 c          620 g          221 t
ORIGIN      
        1 tcccgggccc gagcgagatc accggtacgg gagcgtagcc atgcagatca ccccccgaga
       61 caagaggatc ctgcagatcg cgggggtcgt ggtgcccctg ctgctgctcg tgtacttctt
      121 cgtgctgcga ccggacggcg atgaggatct cgcgcttccc aacggcccca cgggcatcac
      181 cggaagcacc ggcgcgccca cggagtcgcc gagcgtgacg ccgtccccga cgccccgcga
      241 gacgctgccg cccgtgtcgc tggccggatc aagggatccc ttctcgatcc ctcccggtct
      301 cgagacgtcc acgggcggca gcgtcagccc cgccgcgacg gcgccgaccg gcacgacgac
      361 gacgatcccc gcaacgacga cgttcccgcc gccgccgccg cccccgacca cgaccacgac
      421 gaccttcccg ccgccaccgc caccgaccac gaccaccacg cctccgcccg ggggcggggg
      481 aggcggtggt gagcgcccgg ggaacaagat cctggtcggc gggcacaacg tgaagctgct
      541 cggcgtggcc ggcgggggga ggaagctcga cgtcgcggtc gacggcaagg tctacaccgt
      601 gcagccgggg gccacgttcg acgacaactt caagctcgtg aagatcgacg ggaagtgcgc
      661 cacgttcctg ttcggcgacc agagcttcga gctctgcgag aagtaggacc tcgggacgcc
      721 gccgccgcgc cggcgttcgg gttggagccg acggatcggg gagcgctgcc ggaggcaccg
      781 gcgccgcgca cgggggagcg gccgccggtc cgagagggcg gcctcggtga cggggccgcc
      841 ctccaccatc cggggccccg gggcacccgg gtatgcacgc tgggcctcga gccgttccgt
      901 cccggtccga acggcggtgc cgaggaagcg gtctcctatg atgaacccat gttgcgcctg
      961 ctcacggccg gtgaatccca cgggaaggcg ctcctcgggg tgctcgaggg cctgccggcc
     1021 ggcatcccgg tctcgcccaa ggagatcgcg accgagctcg cgcggcgccg tcacgggcac
     1081 gggcggagcg ggcgacagaa gctggagggc gaccggttcg agatcgtgtc cggagtccga
     1141 cacggcctca cgctgggcag tccgatcggc gtcacgatcg agaacgcgga gtgggacgtg
     1201 aagtaccggg acctgatgtc cgtcgagggc gaggcggatc cggcggaacg cctgacgcgg
     1261 ccccggcccg gacacgcgga cctggccggc gccatgaagt acgggttcga tgacgtccgc
     1321 aacgtcttgg agcgcgcgag cgcacgcgag acggccatgc gggtcgcgct cggcgtgttc
     1381 tgcaaggccc tgctcgccga actcgggatc gcggtcgtct cccacgtcgt gcggatcggc
     1441 aaggtcgcgg tgcgctcgac acgcgtgccg cgccccgagg acctcgagac gatcgacgcg
     1501 tcgccggttc gatgcctcga cgaggtggtc gccgaccgga tggtggccga gatcgatcgc
     1561 gcccggaagg cccgcgacac cgtcgggggg gtgttcgagg tgatcgcctt cgggtgccca
     1621 cccgggctcg gctcccacgt ccagtacgac cgcaagctcg atgcgcgtct ggccctgtcg
     1681 ctgatgagca tccagtcggt caag
//
LOCUS       DATBRK010000274         7596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1490186, whole genome shotgun sequence.
ACCESSION   DATBRK010000274 DATBRK010000000
VERSION     DATBRK010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490186"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="VIH70_08220"
     CDS             <1..121
                     /locus_tag="VIH70_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8116384.1"
                     /translation="KKGLADLIVAKHRNGPTDTVPLTFLPHLTQFRNHATAVT"
     gene            118..816
                     /gene="nth"
                     /locus_tag="VIH70_08225"
     CDS             118..816
                     /gene="nth"
                     /locus_tag="VIH70_08225"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY8116385.1"
                     /translation="MTVDRRRVRALARRLERHFGELAPPRASDPLDELILTVQSQHTS
                     DLNAERAFGLLRARYPTWDDVVRAPTGELADTIRSGGLANTKAARLQAILVEIHRREG
                     GYDLSALASRSNAEIAEYLSSLPGVGPKTVAVVMAFAFGRETIPVDTHVHRVATRLGL
                     VPKTNAERAQRLLEELIPDELKTPMHVGLIRLGREICKAGRPRCEDCPMFELCPTGPV
                     VLGVARPPSSTSSS"
     gene            complement(885..955)
                     /locus_tag="VIH70_08230"
     tRNA            complement(885..955)
                     /locus_tag="VIH70_08230"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(921..923),aa:Gly,seq:ccc)
     gene            1043..2071
                     /gene="trpD"
                     /locus_tag="VIH70_08235"
     CDS             1043..2071
                     /gene="trpD"
                     /locus_tag="VIH70_08235"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY8116386.1"
                     /translation="MTEPDPDLWPHVLSKIAAGTSLDRDEAAAAMRVVMSGEATPAQV
                     GGLLMALRTKGETVEEIEGLADTMLGFANPVAPPAPVVDTCGTGGDRSGTFNISTVAA
                     LVVAGAGVPVAKHGNRAASSVCGSADLLEALGVRIDLDATGVERCLAEAGIAFIFAPV
                     FHPAMGHAGPVRRELRVPTVFNFLGPLTNPARPFAQVVGVSDERMLPLLAHVLARRGT
                     RARLFRALDGLDELTTTGPSLVFDVQDGELRESMLDPADLGLARSEVGDLRGGDPEAS
                     AAIAHAIIEGETGPRRDVVLLNAAAALEVSGRASSIDEGLAMAAGSIDSGAAVDVLAR
                     WVSSSGNG"
     gene            2084..2659
                     /gene="dcd"
                     /locus_tag="VIH70_08240"
     CDS             2084..2659
                     /gene="dcd"
                     /locus_tag="VIH70_08240"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY8116387.1"
                     /translation="MILSDVDIRKELAAGRIEIDPFDDASVQPSSVDLHVDRQFRVFA
                     NSRYPYIDVRREMPDLTEMVEVAGDEAFILHPGEFVLGSTIERVALPDDLVARLEGKS
                     SLGRLGLLIHSTAGYVDPGWDGYLTLELSNVANLPITIYPEMKIGQISFFRLSTPAEV
                     PYGATGTKSKYQGQRGPTASRFFEEFGDREA"
     gene            complement(2656..2847)
                     /locus_tag="VIH70_08245"
     CDS             complement(2656..2847)
                     /locus_tag="VIH70_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116388.1"
                     /translation="MSTDRYRTPKEPDQDAYEDAWYRALKAKATRRSGEPKDDTTDAP
                     PAQAFPPAPTQNEGDAGAR"
     gene            complement(2874..3572)
                     /locus_tag="VIH70_08250"
     CDS             complement(2874..3572)
                     /locus_tag="VIH70_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8116389.1"
                     /translation="MKVLVVDDHALVRRGMGHVIRECFSDAEVVEAGSASEALEKLAG
                     GTIDIALVDVRMPDSDGLELLHNVKERWPDLPVIMLTSFDHAHYVRRALAEGAAGYML
                     KDATPEDLEQAINVALSGGGNVLSPKVIQNLFESIDGGVGNDGTPSVRTAGSLTQRET
                     DILALLAEGRSNRDISRALFLSEKTVKAHLAAIFRKLSVTNRTQAAMAAVSMGIGPGL
                     QPVETVGRTGGSDH"
     gene            complement(3569..4303)
                     /locus_tag="VIH70_08255"
     CDS             complement(3569..4303)
                     /locus_tag="VIH70_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8116390.1"
                     /translation="MHHDGRAVEAMTLRGGATSAEGLGRGEVLEVPRDLDGPVDGDRA
                     RLAYAIHDGLTQVVTASVLELESLAHRAEIAPSEAAEALRQAVRELRRALDEIRGVLA
                     NLTEVPPATAQPIEELVRSVLDRWQLPATWSIEGDLAEVSPSVLEAASSVIREGIANA
                     AKHAGGREIAVRVLASDRSVEVSVEDHGRGFESSQHEPAAGHLGLDMLRQRVADVHGT
                     LDVRSSPGKGTRVVARLPRYEQGESP"
     gene            complement(4519..6519)
                     /locus_tag="VIH70_08260"
     CDS             complement(4519..6519)
                     /locus_tag="VIH70_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY8116391.1"
                     /translation="MVVRAAVLALLIVIAGGLAARRARLLTRLVRLGRPVERSGDVSR
                     RVAREGTEVLGQRKLFQRALPGLMHALIFWGFLILLTTIVEVMGQLVDPTFELPLIGG
                     TRWLGLIQDVFAAGVLVGIAIAVGIRVFQRPERFVGSHRWEAYRILGLIFLIIVTLFL
                     ARGARIAAGYAPDWWWTPVSTATSSLFTWMSGGWQRASMGTLQWVHVAIILGFLVYLG
                     YSKHLHIATSAINVYFASTRPRGTLTPLRIDLESAEADDVHLGAATITDLTWKQTLDL
                     YACTECGRCQGACPAWNTGKPLSPKLLVMNLRDHLFERGPQILEARAADTELEPVPLV
                     PEIVDEEVLWACTTCGACMQECPVDIEHVDTIVDLRRNLVMAESRFPAEAGALLRNLE
                     TAGNPWGMPQAQRADWAKGLDVRVIEEGRQAPEYLYWVGCAGAFDDRATTISKAVVRL
                     LQRAGVSFAILGPRELCTGDPARRIGNEYLFQTLAEQNVETLSGAGVTKVIANCPHCF
                     NTLRNEYPDYGGRFEVVHHSQLFAHLVAEGRLRPTEEVSATLTYHDPCYLGRHNGVYR
                     DPRAALEAVPGVRTVEMPRHGDRALCCGAGGARMWMEERIGRRINEERADEAASTGAD
                     TLGVACPYCLIMLDDGERARGGRTEVLDLAQVLARSVAGPQA"
     gene            6830..>7596
                     /locus_tag="VIH70_08265"
     CDS             6830..>7596
                     /locus_tag="VIH70_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY8116392.1"
                     /translation="MARAVGIDLGTTNSVVSVLEAGEPVVIPNAEGSRTTPSVVGFAK
                     NGEILVGEVAKRQAITNPDRTVRSVKRHMGAKDWGPDVDGKRWTPQEISAQILGKLKR
                     DAEAYLGDSVNQAVVTVPAYFDDSQRQATKEAGQIAGLEVLRIINEPTAAALAYGLDK
                     QHDQTILVFDLGGGTFDVSLLEIGEGVFEVKSTHGDTQLGGDDWDQRVIDWMVKEFKN
                     NYGIDLGADRMALQRLKEAAEKAKIELSQVSEATINLP"
BASE COUNT         1139 a         2685 c         2626 g         1146 t
ORIGIN      
        1 caagaagggc ctggccgacc tgatcgttgc caagcatcgc aacggtccga ccgacacggt
       61 accgctcacc ttcctgccgc acctgacgca gttccgcaac cacgccaccg cggtgacgtg
      121 accgtcgacc gccgccgcgt ccgggcgctc gcccgccggc tcgagcggca cttcggggag
      181 ctcgcgccgc cccgagcctc cgacccgctc gacgagctga tcctcacggt gcagtcccaa
      241 cacaccagcg atctgaacgc cgagcgcgcg ttcgggctcc tgcgggcccg ctacccgacg
      301 tgggacgacg tggttcgcgc gcccaccggc gagctcgccg acacgatccg ctcggggggg
      361 ctcgccaaca cgaaggccgc caggctccag gcgatcctgg tcgagatcca tcggcgagag
      421 ggcgggtacg acctgtcggc cctcgcgtcg agatcgaacg ccgagatcgc cgagtacctc
      481 tcgtcgctcc ccggcgtcgg ccccaagacg gtggcggtcg tgatggcgtt cgcgttcggc
      541 cgcgagacga tcccggtcga cacgcacgtg caccgggtgg ccacacggct cggcctggtc
      601 ccgaagacca acgccgaacg cgcgcagcgg ctcctggagg agctcatccc cgacgaactg
      661 aagacgccga tgcacgtcgg cctgatcagg ctcggtcggg agatctgcaa ggcgggccgc
      721 cctcgttgcg aggactgtcc gatgttcgag ctctgcccga cgggaccggt cgtcctcggc
      781 gtcgctcggc cgccgagctc gacctcgagc tcgtagttcg cctcgcggcg ggagcaacag
      841 cctcttggtg cccctcctcg gggcgacgca cctcaactgg acggagcggg cgaggggaat
      901 cgaacccccg tttcaagctt gggaagcttg cgttctacca ttgaaccacg cccgcgcacg
      961 ccgctcgccg gagcgagcgg gtccccagtg tagcgagccc gcggaccgcg tgggaggcgg
     1021 ccctgctagc ctccgatgcc ccatgacgga gccggatccg gacctgtggc cgcacgtcct
     1081 gtcgaagatc gccgccggaa cctccctgga tcgcgacgag gcggcggcgg cgatgcgggt
     1141 ggtgatgtcg ggggaggcga cgcccgcaca ggtcggcggc ctcctgatgg cgctgcgtac
     1201 caagggcgag acggtcgagg agatcgaggg gctcgcggac acgatgctcg ggttcgcgaa
     1261 cccggtcgcc ccgccggctc cggtcgtcga cacctgcggg accggcgggg atcgaagcgg
     1321 gacgttcaac atctcgacgg tcgcggcgct ggtcgtcgcc ggcgccggcg tccccgtcgc
     1381 caagcacggc aaccgggccg cgtcgtccgt ctgtggctcg gcagacctgc tcgaggcgct
     1441 cggcgtccgg atcgacctcg acgcgaccgg cgtcgaacgc tgcctggccg aggccggcat
     1501 cgcgttcatc ttcgcgccgg tgttccaccc ggcgatgggg cacgcgggtc cggtccgccg
     1561 cgagctccgc gtcccgaccg tcttcaactt cctcggcccc ctcaccaatc ccgcgcgacc
     1621 gttcgcccag gtcgtcggcg tgtcggacga acgcatgttg ccgctcctcg cgcacgtgct
     1681 cgctcggcgc gggacgaggg cgaggctgtt ccgggcgctc gacggcctgg acgagctcac
     1741 cacgaccggt ccgtcgctcg tcttcgacgt gcaggacggc gagctacgcg agtcgatgct
     1801 cgaccccgcc gacctcgggc tcgcccggtc cgaggtgggg gacctacgcg ggggcgaccc
     1861 ggaggcgtct gcggcgatcg cccacgcgat catcgagggc gagacaggtc cgcgccgcga
     1921 cgtcgtgctg ctcaacgccg cggcagcgct cgaggtctcg ggcagggcga gctcgatcga
     1981 cgagggcctc gcgatggctg ccgggtcgat cgattcgggc gcggcggtcg acgtactggc
     2041 gcgctgggtg tcgtcctcgg ggaacgggta gcatccggcc gcgatgatcc tgtccgacgt
     2101 cgacatccgc aaggagctgg cggcgggccg catcgagatc gatccgttcg atgacgcatc
     2161 cgttcagccc tcgtcggtcg acctgcacgt cgaccgccag ttccgcgtct tcgcgaactc
     2221 gcggtatccg tacatcgacg tccggcgcga gatgccggac ctgaccgaga tggtcgaggt
     2281 cgccggtgac gaggccttca tcctccatcc cggcgagttc gtgctcgggt cgacgatcga
     2341 gcgggttgcc ctgcccgacg acctggtcgc tcgtctcgag ggcaagtcga gcctcggacg
     2401 cctcgggctg ctgatccatt ccaccgccgg ctacgtcgac ccggggtggg acgggtacct
     2461 gacgctcgag ctgtcgaacg tcgcgaacct gccgatcacg atctacccgg agatgaagat
     2521 cgggcagatc tcgttcttcc ggctgagcac gcccgcggag gttccctacg gagcgacggg
     2581 gaccaagagc aaataccagg gtcagcgagg tccaaccgcc agccggttct tcgaggagtt
     2641 cggcgaccgc gaggcctagc gggccccggc gtcgccctcg ttctgggtcg gcgccggtgg
     2701 gaacgcctgt gccggaggcg cgtcggtcgt gtcgtccttc ggctcgccgg accggcgcgt
     2761 tgccttcgcc ttcagcgcgc ggtaccaggc gtcctcgtag gcgtcctggt ccggctcctt
     2821 cggggtcctg tatcgatcgg tcgacatcgc tcttctcccg tcgtgaacgc tggctagtgg
     2881 tccgatccgc ccgtgcggcc gaccgtttcg accggctgca ggcccggccc gatgcccatg
     2941 gaaaccgccg ccatcgccgc ctgcgtgcgg ttggtgacgc tcagcttgcg gaagatcgcg
     3001 gcaaggtgcg ccttcaccgt cttctccgaa aggaacaacg cgcgcgagat gtctcggttg
     3061 ctccggccct ccgccaggag cgcgaggata tcggtctcgc gctgcgtgag ggacccggcg
     3121 gtccgcaccg acggggtccc gtcgttcccg acgccgccgt cgatcgactc gaacaggttc
     3181 tggatcacct tcggggacag gacgttaccg ccgccggaca gcgcaacgtt gatcgcctgt
     3241 tcgagatcct cgggcgtggc gtccttcagc atgtaccctg ccgcgccctc ggcgagcgcg
     3301 cggcgcacgt agtgcgcgtg gtcgaacgac gtcagcatga tcacgggcag atccggccaa
     3361 cgctccttca cgttgtgcag cagctcgagg ccgtcggagt caggcatgcg aacgtccacc
     3421 agcgcgatgt cgatcgtgcc gccggccagc ttctcgagcg cctcggacgc gctccccgct
     3481 tcgaccacct cagcatccga gaagcactcc cggatcacgt gccccatccc gcggcgcacc
     3541 agcgcatggt catcgaccac gagcaccttc acgggctttc tccttgttcg taacggggca
     3601 gcctcgcgac gacccgcgtt cctttgcccg gcgacgatct gacgtcgagc gtgccgtgga
     3661 cgtcggccac ccgctggcgc aacatgtcca ggcccaggtg accggcggcg ggctcgtgct
     3721 gggagctctc gaaacccctg ccgtggtctt ccacgctgac ctcgaccgaa cgatcggatg
     3781 cgaggacgcg gacggcgatc tcgcgaccgc ccgcgtgctt ggcggcgttc gcgatgcctt
     3841 cccggatgac cgatgacgcg gcctcgagca cggacggtga cacctcggcc aggtcgcctt
     3901 cgatcgacca cgtggccggg agctgccagc gatccagcac gctgcgcacc agctcctcga
     3961 tcggctgtgc ggtggcgggg gggacctccg tcaggttcgc gaggacaccg cggatctcgt
     4021 cgagcgcccg ccgcagctcg cggaccgcct ggcgcagcgc ctcggcggcc tccgacggcg
     4081 cgatctcggc acgatgggcg agcgactcga gctcgaggac ggacgcggtg accacctgcg
     4141 tcagcccgtc gtggatcgcg tacgccagac gagcccggtc gccgtcgacc gggccgtcca
     4201 ggtctcgagg tacctcgagg acctcaccgc gtccgagacc ctcggcggac gtggccccgc
     4261 cccggagcgt catcgcttcc accgccctcc cgtcgtgatg catggctttc tcaccgtgcc
     4321 cgccggttcg gctcgtgcct agatcggcgc acaccttcgg tcgccacccc ccagccaagg
     4381 gatgccgagt ctgttcggtc tagttctcat ttcggtcgtt cggggcggcg gcttgaaccc
     4441 acgctcgggg aggggctgcc aggcttaggc cctccgggcc tcgtggactc cttctgcgtg
     4501 aggggaggga gaccctggtc aggcctgcgg cccggccacc gagcgcgcca gcacctgcgc
     4561 gaggtcgagc acctcggtcc ggccgccgcg ggcccgctcg ccgtcatcga gcatgatcag
     4621 gcagtagggg caggcgaccc cgagtgtgtc ggcgccggtt gaggcggcct cgtcggcgcg
     4681 ctcctcgttg atccgcctgc cgatgcgctc ctccatccac atgcgagccc ctccggcccc
     4741 gcagcacaac gcccggtccc cgtgacgcgg catctcgacc gtccggacgc cgggcaccgc
     4801 ctccagcgcc gcgcgaggat cgcgatagac accgttgtgg cgccccaggt aacacgggtc
     4861 gtggtaggtg agcgtggcgg agacctcctc ggtcggcctc aggcgcccct cggcgaccag
     4921 gtgcgcgaac agctgcgagt ggtggaccac ctcgaaccgt ccgccgtagt cggggtactc
     4981 gtttcgcagg gtgttgaagc agtgcgggca gttcgcgatc accttcgtga cgccggcgcc
     5041 cgagagcgtc tccacgttct gctcggccag ggtctggaag agatactcgt tgccgatccg
     5101 acgcgcgggg tcgccggtgc agagctcgcg cggcccgagg atcgcgaacg acacgccggc
     5161 ccgctgcagc agccgcacga ccgccttgga gatcgttgtc gctcggtcgt cgaacgcccc
     5221 agcgcacccg acccagtaca ggtactcggg cgcctgccgg ccctcttcga tcacgcggac
     5281 atccagaccc ttggcccagt ccgctcgctg tgcttggggc atgccccacg ggttgccggc
     5341 agtctcgagg ttgcgcagca gggcccccgc ctcggccggg aaccgagact ccgccatcac
     5401 gaggttgcgg cgtaggtcga cgatcgtgtc gacgtgctcg atgtcgaccg ggcactcctg
     5461 catgcaggcg ccgcaggtcg tacaggccca caggacctcc tcgtcgacga tctcggggac
     5521 gagggggacc ggttcgagct cggtgtcggc cgccctggct tccaggatct gggggccccg
     5581 ctcgaagagg tgatcccgca ggttcatgac caggagcttg ggcgagagcg gcttgcccgt
     5641 gttccacgcc gggcacgctc cctgacagcg cccgcactcg gtgcacgcgt acaggtccag
     5701 ggtctgcttc cacgtgagat ccgtgatcgt ggccgcgccg aggtgcacgt cgtccgcctc
     5761 ggcgctctcg agatcgatcc ggagcggggt cagggtgccc cgcggtcggg tgctcgcgaa
     5821 gtagacgttg atcgcgctcg tggcgatgtg caggtgcttc gagtacccca ggtagacgag
     5881 gaatccgagg atgatcgcga cgtggaccca ctggagggtc cccatcgagg cccgctgcca
     5941 cccgccggac atccaggtga acagggacga cgtggccgtc gacacgggcg tccaccacca
     6001 gtccggcgcg tacccggccg cgatccgtgc gccccgcgcg aggaacaggg tgacgatgat
     6061 caggaagatc aggccgagga tccggtaggc ctcccaccga tggctcccga cgaagcgctc
     6121 ggggcgctgg aacacccgga taccgaccgc gatcgcgatc ccgaccagca cgccggccgc
     6181 gaacacgtcc tggatcaggc ccagccatcg cgtgccgccg atcaggggga gttcgaaggt
     6241 cgggtcgacc agttgtccca tgacctcgac gatcgtggtg agcaggatca ggaaccccca
     6301 gaagatcagc gcgtgcatca gtccggggag cgcgcgttgg aacagcttgc gttgcccgag
     6361 gacctccgtg ccctcacgag cgacgcggcg ggacacgtca ccgctgcgct cgaccgggcg
     6421 tccgagtcgc acgagccgcg tgagcaggcg tgcccggcgg gccgcgagcc cgcccgcgat
     6481 gacgatcagg agcgccagga ccgcagctcg cacgaccatc ggcgcgagca tacccggcgc
     6541 ctgcgggacg cttgaccgcg gggctgccgg ggcatcatgg ggccggaccg atcgcagccg
     6601 tcgtgctcgg gatcgccccg ctcgccgggg cgatggttcg ctacccggat cggggccggc
     6661 aagtcgggga agcccacacc gccgatgacc gaagacctcc tgcccgaaca cgactctgtg
     6721 ggatcctcgg ctccggaata ccttcccccg ggcacgaggt tgatacccat gcgctcaagg
     6781 ttctatattc tgagcgactg aaggacgtcc gagcgaagga ggcaacgcga tggcacgtgc
     6841 ggtggggatc gacctgggga cgacgaactc ggtggtctcg gtgctcgagg ccggcgagcc
     6901 cgtcgtcatc ccgaacgccg aaggctcccg taccaccccc tcggtggtgg ggttcgccaa
     6961 gaacggcgag atcctggtcg gggaggtcgc caagcgccag gccatcacga acccggatcg
     7021 aacggtccgg tccgtgaagc ggcacatggg cgcgaaggac tggggccccg atgtcgacgg
     7081 caagaggtgg acgccgcagg agatctccgc gcagatcctc ggcaagctga agcgtgacgc
     7141 cgaggcgtac ctgggcgact cggtgaacca ggccgtggtc acggtgcccg cgtacttcga
     7201 cgactcgcag cgccaggcca ccaaggaggc cgggcagatc gcggggctcg aggtgctccg
     7261 catcatcaac gagccgacgg cggcggcgct cgcgtacggg ctcgacaagc agcacgacca
     7321 gacgatcctc gtgttcgacc tggggggcgg gacgttcgac gtctcgctgc tcgagatcgg
     7381 cgagggcgtg ttcgaggtca agtccaccca cggcgacacc cagctgggcg gcgacgactg
     7441 ggaccagcgc gtgatcgact ggatggtcaa ggagttcaag aacaactacg ggatcgacct
     7501 cggggccgac cgcatggcgc tccagcgcct caaggaggcc gccgagaagg cgaagatcga
     7561 gctctcgcag gtgagcgagg ccacgatcaa cctgcc
//
LOCUS       DATBRK010000275         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2209498, whole genome shotgun sequence.
ACCESSION   DATBRK010000275 DATBRK010000000
VERSION     DATBRK010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2209498"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /gene="rplV"
                     /locus_tag="VIH70_08270"
     CDS             complement(<1..338)
                     /gene="rplV"
                     /locus_tag="VIH70_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY8116393.1"
                     /translation="MEAKATVKYVRISPPKARRAVDLVRGRQVEEARRILRFSPLGAA
                     KTIEKALASAVANAEQQPGVVAQNLVVDRAWVDEGPTLKRWRPRAYGRATKILKRTSH
                     ITLVVRSLGE"
     gene            complement(346..627)
                     /gene="rpsS"
                     /locus_tag="VIH70_08275"
     CDS             complement(346..627)
                     /gene="rpsS"
                     /locus_tag="VIH70_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY8116394.1"
                     /translation="MARSIKKGPFVDEHLMVKIVEMNRKSDKKVVRTWSRRSTIVPEM
                     VGHTIAVHDGRKHVPVYISESMVGHKLGEFAPTRTFRSHARGERSTTVR"
     gene            complement(627..1457)
                     /gene="rplB"
                     /locus_tag="VIH70_08280"
     CDS             complement(627..1457)
                     /gene="rplB"
                     /locus_tag="VIH70_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY8116395.1"
                     /translation="MAVRKYKPTTPGRRGASVSSFEEITRGKPERSLVGKGRNRAGRN
                     SQGRVTTRHQGGGNKRRYRVIDFRRDKDGVPAKVAHIEYDPNRTARIALLHYADGEKR
                     YILSPNGLRVGDRLASGEGADVRPGNSLPLRNIPVGTTVHAVELKPGGGARMARSAGT
                     SIQVMAKEGKMATLRLPSGETRLVEATCKATIGEVGNAEHELIQLGKAGRNRWKRKRP
                     SVRGVAMNPVDHPLGGGEGKSSGGRHPTSPWGKKEGRTRKKNKASSKYIVRRRGKGRG
                     "
     gene            complement(1461..1751)
                     /gene="rplW"
                     /locus_tag="VIH70_08285"
     CDS             complement(1461..1751)
                     /gene="rplW"
                     /locus_tag="VIH70_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019354950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY8116396.1"
                     /translation="MKTPRDVIIRPVVSEKSYAGLEQNTYTFLVDPRANKTEIKEAVQ
                     VIWNVQVISVRTLNRTGKVKRRRYTLGKRADQKRAIVKLVEGDSIEIFETGK"
     gene            complement(1748..2458)
                     /gene="rplD"
                     /locus_tag="VIH70_08290"
     CDS             complement(1748..2458)
                     /gene="rplD"
                     /locus_tag="VIH70_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY8116397.1"
                     /translation="MPKVDVVSVAGKKVGTRDLAPEVFEAKVSVPLMHQVVLAGFASI
                     RRGTHSTKTRGEVSGGGRKPWRQKGTGRARQGSNRAPQWTGGGVVHGPRPRDHQMRVN
                     KKMRRGALRSALTDALTSGKLSVVQDLAFDAPKTKTAAEVLDTLELAGRILLVLPSPS
                     ESGAVEKSFRNIRGVRVAYARSLGVYEVIGADHLVITESALDVVEGSPAPPEEAAAET
                     PAKSAQAEPTTDTGGEAE"
     gene            complement(2458..3114)
                     /gene="rplC"
                     /locus_tag="VIH70_08295"
     CDS             complement(2458..3114)
                     /gene="rplC"
                     /locus_tag="VIH70_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY8116398.1"
                     /translation="MAEIKGILGTKLGMTQIFDDTRAIPVTVIKAGPCFVAQVKTEEK
                     DGYSAVQLAFGATRPNELNKPEQGHFDKHGGQIGRHVVEIRTDDAGSYEPGQELRADV
                     FEAGELADVVGVSKGKGFAGVMKRHGFSGLSSSHGTERKHRSPGAIGACATPSRVFKG
                     MRMAGQMGNERVTVLNLEVVRADAERNLLLLRGAIPGAEGGLVMVRSAVKAPAAKGGR
                     "
     gene            complement(3119..>3313)
                     /gene="rpsJ"
                     /locus_tag="VIH70_08300"
     CDS             complement(3119..>3313)
                     /gene="rpsJ"
                     /locus_tag="VIH70_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY8116399.1"
                     /translation="PVPLPTERSIYTVIRSPHKYKDSREHFQMLTHKRLIDIVDATSK
                     TVQELQRLNLSAGVDIEIKL"
BASE COUNT          421 a         1216 c         1077 g          599 t
ORIGIN      
        1 tcctctccca acgaacggac cacgagcgtg atgtgcgacg tgcgcttgag gatcttggtc
       61 gcgcgaccgt aggcgcgcgg gcgccagcgc ttcagcgtcg gaccctcgtc gacccacgcg
      121 cggtccacga ccaggttctg cgcgaccacg cccggctgct gctcggcgtt cgcgacggcc
      181 gaggccaacg ccttctcgat cgtcttggcg gcgccgagcg gcgagaagcg caggatccgg
      241 cgggcctcct cgacctggcg gccgcggacg aggtccaccg cccgccgagc cttcggcggc
      301 gagatccgca cgtacttcac cgtcgctttc gcctccatgt ccccgctacc tcaccgtggt
      361 cgaacgctct ccgcgcgcgt gcgagcggaa cgtccgggtc ggcgcgaact cgccgagctt
      421 gtgcccgacc atcgactccg agatgtacac gggcacgtgc ttgcgtccgt cgtggaccgc
      481 gatcgtgtga ccgaccatct cggggacgat cgtcgaacgg cgcgaccagg tcctgacgac
      541 cttcttgtcg ctcttgcggt tcatctcgac gatcttgacc atgaggtgct cgtcgacgaa
      601 cggccccttc ttgatgctcc gcgccactat ccccgcccct tcccgcgacg gcgaacgatg
      661 tacttgctcg acgccttgtt cttcttcctc gtgcggcctt ccttcttccc ccacgggctc
      721 gtggggtgac ggccacccga actcttgccc tcgccgccgc ccagcgggtg gtcgaccggg
      781 ttcatcgcca cacctcgtac ggaaggacgc ttgcgcttcc agcggttgcg gccggccttg
      841 ccgagctgga tcagctcgtg ctcggcgttg ccgacctcgc cgatcgttgc cttgcaggtc
      901 gcctcgacca ggcgggtctc gcccgagggc agacgcagcg tggccatctt gccttccttg
      961 gccatcacct ggatgctcgt gccggccgag cgcgccatgc gggcgccccc acccggcttg
     1021 agctccaccg cgtgcaccgt cgtgccgacg gggatgttcc gcagcggcag cgagttgccg
     1081 ggccgcacgt cggcgccttc ccccgacgcg agccggtcgc cgacccgaag gccgttcggc
     1141 gacaggatgt agcgcttctc gccgtccgcg tagtgcagca gcgcgatccg cgcggtgcgg
     1201 ttcgggtcgt actcgatgtg cgccaccttg gccggcaccc cgtccttgtc acgtcggaag
     1261 tcgatcaccc ggtaccggcg tttgttgcca ccgccctggt gccgcgtggt cactcggccc
     1321 tgcgagttcc ggccggcgcg gttgcgcccc ttgccgacga gcgagcgctc gggcttgccc
     1381 cgcgtgatct cctcgaacga ggacacgctg gcgccgcgtc gtcccggcgt cgtcggcttg
     1441 tacttgcgaa cggccatccg ctacttcccc gtctcgaaga tctcgatgct gtcgccctcg
     1501 acgagcttga cgatcgcgcg cttctggtcc gctcgcttgc cgagcgtgta gcgccgccgc
     1561 ttgaccttgc cggtgcggtt cagcgtccgg accgagatca cctggacgtt ccagatcacc
     1621 tggacggcct ccttgatctc ggtcttgttc gccctggggt cgaccaggaa cgtgtaggtg
     1681 ttctgctcca ggcccgcgta ggacttctcg ctcaccacgg gccggatgat cacgtcgcgg
     1741 ggggtcttca ctcggcctcc cccccggtgt ccgtcgtcgg ctcggcctgg gcgctcttcg
     1801 ccggcgtttc ggcggcggcc tcctcgggcg gggccgggga gccctcgacg acgtcgagcg
     1861 cgctctcggt gatcacgagg tggtccgcgc cgatcacctc gtagacgccc aggctccgcg
     1921 cataggcgac ccggacaccc cggatgttgc ggaaggactt ctccaccgca ccggactcgc
     1981 tcggcgaggg caggacgagc aggatcctgc cggcgagctc gagcgtatcg agcacctcgg
     2041 ccgcggtctt cgtcttcggc gcgtcgaacg cgagatcctg cacgaccgag agcttcccgc
     2101 tcgtcagcgc gtccgtcagc gccgaacgca gggcaccccg cctcatcttc ttgttcacgc
     2161 gcatctggtg atcgcgcggc cgggggccgt gcaccacgcc gccgcccgtc cactgagggg
     2221 cacggttcga gccctgccgg gcccggccgg tgcccttttg ccgccagggc tttcggccgc
     2281 cacccgaaac ctcgccgcgg gtcttggtcg agtgcgtgcc acggcggatc gaggcgaacc
     2341 ccgcgagcac cacctggtgc ataaggggca cgctcacctt ggcctcgaag acctcgggcg
     2401 ccagatcgcg ggtgccgacc ttcttgccgg cgacggagac cacgtcgacc ttcggcatca
     2461 gcggccgccc ttcgcggcgg gagccttcac ggccgagcgg accatcacca ggccgccctc
     2521 ggcacccggg atcgcgcccc gcaggagcag caggttgcgc tcggcgtcgg cgcgaacgac
     2581 ctcgaggttg agaactgtca cgcgctcgtt gcccatctgt cccgccatcc gcatcccctt
     2641 gaacacgcgc gagggagtcg cgcacgcccc gatcgcgccc ggcgagcggt gcttgcgctc
     2701 ggtaccgtgg ctgctcgaca ggccgctgaa cccgtgccgc ttcatcacgc cggcgaatcc
     2761 cttgcccttg gagacgccga cgacgtcggc gagctcgccg gcctcgaaca cgtcggcccg
     2821 cagctcctgg cccggctcgt acgagccggc gtcgtccgtg cggatctcga cgacatgccg
     2881 gccgatctgg ccgccgtgct tgtcgaaatg accctgctcg ggcttgttca gctcgttcgg
     2941 gcgcgtggcg ccgaacgcaa gctggaccgc cgagtatccg tccttctcct cggtcttgac
     3001 ctgcgcgacg aagcacggcc cggccttgat gaccgtgacc gggatcgcgc gcgtgtcgtc
     3061 gaagatctgg gtcatgccca gcttcgtccc gaggatgcct ttgatctctg ccatgtccct
     3121 acaacttgat ctcgatgtcg acgccggccg acaggttcag acgctggagc tcctggaccg
     3181 tcttcgacgt ggcgtcgacg atgtcgatga gccgcttgtg cgtcagcatc tggaagtgct
     3241 cgcggctgtc cttgtacttg tggggggagc ggatcacggt gtagatcgac cgctcggtcg
     3301 gcagcggcac ggg
//
LOCUS       DATBRK010000276         7588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_330303, whole genome shotgun sequence.
ACCESSION   DATBRK010000276 DATBRK010000000
VERSION     DATBRK010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330303"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VIH70_08305"
     CDS             <1..211
                     /locus_tag="VIH70_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY8116400.1"
                     /translation="ARRIGKKKAAIAVGHSILVIAWYLLANDCDYQDLGGDYFVRRDA
                     DRARQRAVAQLQALGYRVTLEPLAA"
     gene            614..1567
                     /locus_tag="VIH70_08310"
     CDS             614..1567
                     /locus_tag="VIH70_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116401.1"
                     /translation="MIELEDIAYVRSGVTDLPAAVRFATDIVGLELVASDEQGVAHLR
                     ADARHHCLALVEGRSGVIASGFTVADDGALAGAERELERRGIVVRRLDAAAARSRRVG
                     SGIAFDDPFGNRLELVTQQECLARPVAFGRASGITEFGHLCLDAPDVHEAYRFWSGTF
                     NAKVSDWIGDAACLMRIDPVHHKLAVFKGDGPGLCHMNFQVASIDDVFRNWHFLVDHG
                     VEIEMGPGRHPQSTAVFIYFLGPESFTYEYSFGVRRIEDDAGWRPRTFDPAEPGSIDM
                     WLGPTSRPTTQPQARDDLPDGAGPGAPSEDPARVPAALPAS"
     gene            complement(1564..2340)
                     /locus_tag="VIH70_08315"
     CDS             complement(1564..2340)
                     /locus_tag="VIH70_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8116402.1"
                     /translation="MARSVARIPGIERVDLPEVRQAIPRLHSYLRECLLDGRIPPGTK
                     LSQVALAEQLGVSRTPLREVLRMLQEEGYVEFEPNQRMRVADLDPVQLDADYACRILL
                     ETLALSMTLESIGPRERREARRLLTAMRRTARIGDLPGWFEAHHAYHRVMTTGAGKPL
                     LRTLQSLEDRSGRYIRIYQRSEPGDWQTAGDAEHAAILDALAAGDGSAALSGLAHHLA
                     RTALRVLSEHAPDYTPRAVPNAVALAEGTDPAALRAASAG"
     gene            2449..2814
                     /locus_tag="VIH70_08320"
     CDS             2449..2814
                     /locus_tag="VIH70_08320"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8116403.1"
                     /translation="MPVAGSAMLGAQLWVVRWTPRDGVTAADVEAALDAHLNWMLGLE
                     AAGHVVASGPLTSGPGVAPGAGLTVLRASTERDAVALAAQDPFVAKGLRTFDVLGWRV
                     MEGALTVRVSFGTATYRIS"
     gene            2846..3742
                     /locus_tag="VIH70_08325"
     CDS             2846..3742
                     /locus_tag="VIH70_08325"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8116404.1"
                     /translation="MTTVGVIGLGKMGLPIAQNLMERGFAVVGYRRSGTGEFVALGGT
                     GAQSPAEVASASDVLLSILPDVAALREVVLGPQGTLTKLRPGTVHVEMSTLDIVEKAV
                     LRDRVRTCGSDLLDCPISGTPAMVRPRAATTFVSGEKASVDAVREVLDAISGPWVYTG
                     AFGTAAHLKYVASLLVAVHTVATAEAMLLARRSGLDLELVQRTLDDSIASSAIWQRRG
                     PLVRERRWTPAPGPIATLHEILEQIEDQAVSTGTALPLFAVAKQLYDKALADGWGALD
                     IAAVHDLLSGQSPASAEPGSAS"
     gene            3739..4665
                     /locus_tag="VIH70_08330"
     CDS             3739..4665
                     /locus_tag="VIH70_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8116405.1"
                     /translation="MTTWSLVTVVDDGEPFIAVRVGDGNLREVPALKRWASLTELVDG
                     WPAATEALRAIDVEGAAPLAGVRLLAPLRYPRKVICAGVNYRRHIAEMGTELPPADWT
                     PFFFLKPPTTTVVGPFDDVPVDGPEAARLDWEAELAVVIGVGGRDIPPAQALAHVAGY
                     CVANDITARGYHRRANVPADAFRYDWFASKARDGSLPLGPGLTPAWLIPDPQDLRIRL
                     WVNDELQQDGSTSDMICDIPTLVAAASQAVTLEPGDVISTGTPAGVGAGRGVSLRPGD
                     VVRTEIEQLGVLVNRIVATDPPTPPRSRLEEP"
     gene            4662..5123
                     /locus_tag="VIH70_08335"
     CDS             4662..5123
                     /locus_tag="VIH70_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8116406.1"
                     /translation="MIFVSHALAVNEEGQPHLDRAALWNGLVLKADNALPFVPAMTRC
                     DIVERYGPASFDRDIEFRGQQFRERITLEEPHRVVFTRISGPVLGTIANEIEGPDDAL
                     QLRFSFALVVAGVQGGSQAEREYAEGMTADYLKAVAATLDAMRRIARGEAA"
     gene            5141..5548
                     /locus_tag="VIH70_08340"
     CDS             5141..5548
                     /locus_tag="VIH70_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8116407.1"
                     /translation="MSTWIDDYYADVDAMRLEAFVERHSDDATVVFGNNPPSVGKEAI
                     RAAIGGFWSMIGGLRHERRNLWFINDGDTAVLEAVTHYTTKGGAKVPVPCVSLLDRNA
                     DGKVSSLRFYIDLAPLFERIGAEQADRPETVTA"
     gene            5631..7061
                     /locus_tag="VIH70_08345"
     CDS             5631..7061
                     /locus_tag="VIH70_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8116408.1"
                     /translation="MALTSSAAIDQELRLRIADQLGAEFKGDVIGPDHPGYGAARTVW
                     NAMVDKGPGVILLCTSTADVVAAVNVARANGLPPSIRCGGHSVSGKALSDGGLTIDLS
                     GMREVTVDAEHRLVHAGGGCLLGDLDEATAPHGLIVPAGIMSETGVAGLALGGGIGWF
                     SRKHGLTCDQFVSLELVLASGEVINVSATEHPELFWALKGGGGNFGVVTRFTFRAHEF
                     GPTMRLGVSLYEPEDATQALREYAAIVPTLPRTVGWHAALKHDMPALPFVPPELVGRR
                     LLMLISMWLDDADDPAGAELLDRLVTVGQPCVKATTAMPFAARVQKVIDEEFEDGHRY
                     YTKEAHVSDLADEAIDTLVAFWKDMPMHGEVEIIGLGGAIGDVPEDAMAFSNRGYLLW
                     LNFAMRWDDPGNDQDHIERTRKIVADLKPWTGKGIYVNMLNFDEMDRVVEAFGGPEKY
                     ARLGRVKAQYDPENLFRVNYNITPVS"
     gene            7061..>7588
                     /locus_tag="VIH70_08350"
     CDS             7061..>7588
                     /locus_tag="VIH70_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116409.1"
                     /translation="MQPDVALTLVIPLRAKSGMSLPDFYQYWLNAHVTLPPRFPGISS
                     IWLHVVSFDRQRWPRLPGVSHRPEPQDEFHGVPEATFPTMADLELFQSYAHVQMVDGI
                     NFLGEVITYSSLGGNSATLVDKLGDAAPDGHDDLVRHLLFLRRRADVPAAALRRFVTE
                     TLEPAYAGSPEVLKLR"
BASE COUNT         1102 a         2736 c         2621 g         1129 t
ORIGIN      
        1 ggcccgacgc atcggcaaga agaaggcggc catcgcggtc ggccactcga tcctggtgat
       61 tgcctggtac ctgctcgcca atgactgtga ctaccaggac ctgggtggcg actacttcgt
      121 ccgccgcgac gccgaccgcg ctcgccagcg agccgttgcc caactccagg ccctcggcta
      181 ccgcgtcacc ctcgaaccac ttgccgccta acaccccggg ggattcacct ttcaggatga
      241 ggccggaggt ccaagtcctc ccaggcccac taccagcgac aaccagtgag aacgctagtc
      301 acagtttgcg ctcggagccg ttcggcgggc cgtgtaccgg aggacacctc ataactggct
      361 taacttcctg aggtgtcatg gacacatcga cctgctcccc tcctgcgctc cgtcgagtgg
      421 cacgcgcgag aatcgctcgg aacgcatcac caccatctac gtgatcgacc tatgccaagc
      481 ggagaccttc ctgcgggccc gcgaaccctt gtccgccgac gagtcgagac ccggctcgaa
      541 cccggtatgg ccactgaggg cttgccccga tccgaggaaa ctgtgtacag tttccgagga
      601 agggaggctg acgatgatcg agctcgagga catcgcctac gtgcgttccg gcgtcacgga
      661 tctgccggcc gccgtccgct tcgcgaccga catcgtgggg cttgagctgg tggcctcgga
      721 tgagcaggga gttgcgcacc tgcgggctga cgcgcgccac cattgcctcg ctctggtcga
      781 gggtcggtcc ggggtgatcg cgtccggctt caccgtcgct gacgatggcg ccttggccgg
      841 cgcggagcgc gagctcgaac gtcgtggcat cgtggtccgg cgtctcgacg ccgcggccgc
      901 gcggtcgcgc cgggtcggtt ccggcatcgc gttcgacgac cccttcggca atcggctgga
      961 gctggtgacg cagcaggagt gcctcgcccg gccggtcgcc ttcgggcgcg cgtcggggat
     1021 cacggagttc ggtcacctgt gcctggacgc gccggacgta cacgaggcct accggttctg
     1081 gtccggcacc ttcaacgcca aggtgtccga ctggatcggc gatgcggcat gcctcatgcg
     1141 gatcgacccc gtgcaccaca agctcgcggt cttcaagggc gacgggccgg ggctgtgcca
     1201 catgaacttc caggtcgcct cgatcgacga cgtcttccgg aactggcact tcctggtcga
     1261 ccacggcgtc gagatcgaga tggggccggg ccggcaccca cagtcgaccg ccgtgttcat
     1321 ctacttcctc ggtcccgaga gcttcaccta cgagtactcg ttcggggtgc gccggatcga
     1381 ggacgacgcg gggtggcggc cacggacctt cgatcccgcc gagcccgggt cgatcgacat
     1441 gtggctgggg ccgacgtcgc gaccgacgac gcagccgcag gcccgcgacg acctgccgga
     1501 cggcgctggg ccaggggcgc cgtccgagga tccggcgcgg gtgcccgccg cgctcccggc
     1561 gtcttagccc gcggacgcgg cacgcagcgc cgctgggtcg gtgccctcgg cgagggcgac
     1621 ggcgttcggg acggcccgcg gtgtgtagtc cggagcatgc tccgagagca cccgcagcgc
     1681 cgtgcgggcg aggtggtggg cgagcccgga gagggccgcg ctgccgtccc cggccgcgag
     1741 ggcgtccagg atggcggcgt gctcggcgtc acccgcggtc tgccagtcgc ccggctcgga
     1801 ccgttggtag atccggatgt agcgcccact gcggtcctcg agcgactgca gcgtgcgcag
     1861 cagcggcttg cccgccccgg tcgtcatcac ccggtggtag gcatggtgcg cctcgaacca
     1921 cccgggaagg tccccgatcc gcgcggtccg gcgcatcgcg gtgagcagcc ggcgcgcctc
     1981 ccggcgctcc cttgggccga tggactccaa ggtcatcgac agcgcgagcg tctccagcag
     2041 gatgcggcag gcgtagtcgg cgtcgagctg gaccgggtca aggtcggcca cgcgcatccg
     2101 ctggttcggc tcgaactcga cgtagccctc ctcctggagc atccgcagca cctcgcgcag
     2161 cggcgtgcgg ctcacgccga gctgctcggc gagcgccacc tgcgacagct tcgtgcccgg
     2221 cgggatgcgc ccgtcgagga ggcactcgcg caggtagctg tgcaggcggg ggatggcctg
     2281 ccggacctcg ggaaggtcca cgcgctcgat cccggggatc cgggctaccg atcgtgccat
     2341 aggcgcattg ttccacggag cctcaactgc atacacatct tcgccggagt cttgacgatc
     2401 caggacgaaa gtgcatacag tttgcttgag ctctcgagga ggtgcccgat gccggtcgca
     2461 ggctcggcga tgctcggcgc gcagctctgg gtcgtccggt ggaccccgcg cgatggggtc
     2521 acggccgccg acgtcgaggc cgcgcttgac gcccacctga actggatgct tgggctggag
     2581 gccgcggggc acgtcgtcgc ctccggcccg ctgacctcgg ggccgggcgt cgcgccggga
     2641 gcgggcctca cggtgctcag ggcgtcgacg gagcgggacg ctgtcgcgct cgcggcccag
     2701 gacccgttcg tcgccaaggg gctccgcacc ttcgacgtcc tcgggtggcg ggtcatggag
     2761 ggcgcgctca ccgtgcgggt ctcctttggc acggcgacct accggatcag ctgatggccg
     2821 cgggccggcc ggagggaggc ggaccatgac gaccgtcggg gtcatcgggc tcgggaagat
     2881 gggcctgccc atcgcccaga acctcatgga gcgtggcttt gccgtggtgg gctaccggcg
     2941 ctcgggcacc ggcgagttcg tcgccctcgg cggcaccggt gcccagtcac cggccgaggt
     3001 tgcgtccgcc agcgacgttc tgctgtcgat cctgccggac gtcgcggcgc tgcgggaggt
     3061 cgtcctcggc ccgcagggca ccctgaccaa gctgcgccca ggcacggtcc atgtggagat
     3121 gagcactctc gacatcgtgg agaaggcggt cctgcgcgac cgcgtccgga cctgcggcag
     3181 cgacctgctc gactgcccga tcagcggcac gccggccatg gtccgacccc gtgcggcgac
     3241 aaccttcgtc tcgggggaga aggcgagcgt ggacgccgtc cgcgaggtgc tcgacgcgat
     3301 ctccgggccg tgggtctaca cgggcgcctt cggcaccgcg gcgcacctga agtacgtcgc
     3361 cagcctcctg gtcgcggtgc acaccgtcgc caccgcggag gcgatgctgc tggcccgccg
     3421 cagcggcctg gacctcgagc tggtccagcg gaccctggac gactcgatcg cctcctcggc
     3481 gatctggcag cgccgcggcc cgctcgtccg cgagcggcgc tggacgcccg cgcccggacc
     3541 catcgcgacg ctccacgaga tcctcgagca gatcgaggac caggccgtat cgaccgggac
     3601 ggcgctgccg ctgttcgcag tcgccaagca gctgtacgac aaagcgctgg ccgacgggtg
     3661 gggagctctc gacatcgcgg ccgtccacga cctcctgtcc ggccagagcc cggcgtccgc
     3721 cgagcccggg agcgcctcgt gaccacctgg tcgctcgtca ccgttgtcga cgacggcgag
     3781 ccgttcatcg ccgtccgggt gggcgacggg aacctgcggg aggtgccggc gctcaagcgg
     3841 tgggcgagcc tcaccgagct cgtcgacggc tggcccgcgg ccaccgaggc ccttcgcgcc
     3901 atcgacgtcg agggcgcggc cccgctggcc ggcgtccggc tgctcgcgcc gctgcgctac
     3961 ccgcgcaagg tgatctgcgc cggcgtcaac taccggcggc acatcgccga gatgggcacc
     4021 gagctgccac cggcggactg gacgccgttc ttcttcctca agccgccgac cacgacggtc
     4081 gtgggcccct tcgacgacgt tcccgtcgac ggtccggagg cggcgcggct ggactgggag
     4141 gccgagctcg ccgtggtcat cggcgtcggc ggccgggaca tcccgcctgc gcaggccctc
     4201 gcgcacgtcg cgggatactg cgtggcgaac gacatcaccg cgcggggcta ccaccgccgg
     4261 gcgaacgtgc ccgcagatgc cttccgctac gactggttcg cctccaaggc ccgcgacggc
     4321 tcgctgccgc tcggccccgg cctgaccccg gcctggctca tcccggaccc gcaggaccta
     4381 cggatccgcc tgtgggtcaa cgacgagctc cagcaggacg ggagcacgtc ggacatgatc
     4441 tgcgacatcc ccacgctggt cgcggcggcc agccaggccg tgaccctgga gccgggcgac
     4501 gtcatctcca ccgggacgcc ggccggggtc ggcgccggcc gtggcgtgag cctgcggccc
     4561 ggcgacgtgg tccgcacgga gatcgagcag ctcggcgtcc tcgtcaaccg catcgtcgcc
     4621 accgacccgc cgaccccgcc ccgctcgcgc ctggaggagc catgatcttc gtcagtcacg
     4681 cgctggccgt caacgaggag ggccagccgc acctggaccg cgcggccctg tggaacggcc
     4741 tggtgctcaa ggccgacaac gccctgccct tcgtccccgc gatgacgcgg tgcgacatcg
     4801 tggagcggta cggaccggcg agcttcgacc gcgacatcga gttccgcggc cagcagttcc
     4861 gcgagcgcat caccctcgag gagccccacc gcgtcgtctt cactcgcatc tcaggacccg
     4921 tcctcggcac gatcgccaac gagatcgagg gcccggacga tgctctccaa ttgcgtttca
     4981 gcttcgcgct tgtcgtcgcg ggcgtccagg gcggctcgca ggccgagcgc gagtacgccg
     5041 aggggatgac ggcggactac ctcaaggccg ttgccgccac gctggacgcg atgcgccgca
     5101 tcgcccgcgg cgaagccgcc tgacctccgg aggaggagcc atgtccacct ggatcgacga
     5161 ctactacgcc gacgtcgacg ccatgcgcct cgaggcgttc gtcgagcggc acagcgacga
     5221 tgcgaccgtg gtgttcggca acaacccgcc ctccgtcggc aaggaggcga tccgtgcggc
     5281 gatcggcggc ttctggtcga tgatcggcgg gcttcgccac gagcggcgca acctctggtt
     5341 catcaacgat ggcgacacgg ccgtgctgga ggccgtcacc cactacacca caaagggcgg
     5401 cgccaaggtc cccgtaccct gcgtctccct gctggaccgg aacgccgacg gcaaggtgag
     5461 ctctctgcgg ttctacatcg acctggcccc gctgttcgag cggatcggcg cggagcaggc
     5521 cgaccgaccg gagacggtga ccgcttgagc gggcggcacg acgggcgcgt tgccgacggc
     5581 ggtctcgtgc gggcctgacc cttccagacc ggagcaggag gcaaggtcac atggctttga
     5641 cgtcatccgc cgccatcgat caggagctgc ggctccgcat cgccgaccag ttgggcgccg
     5701 agttcaaggg cgacgtcatc ggcccagacc accccggcta cggcgccgcg cgcacggtgt
     5761 ggaacgccat ggtcgacaag ggccccggcg tgatcctgct ctgcaccagc accgcggacg
     5821 tggtcgccgc cgtcaacgtc gcgcgcgcca acgggctccc gccctccatc cgctgcggcg
     5881 ggcactcggt gtcgggcaag gcgctgtcgg acggtggcct gaccatcgac ctgagcggga
     5941 tgcgcgaggt gacggtcgac gccgagcaca ggctggtcca cgccggcggc ggctgcctgc
     6001 taggcgacct cgacgaggcc accgcgcccc acgggctgat cgtccccgcc gggatcatgt
     6061 cggagaccgg tgtcgccggc ctggcgctcg gcggcgggat cggttggttc tcccgcaagc
     6121 acggcctgac ctgcgaccag ttcgtctccc tcgagctcgt gctggccagc ggggaggtca
     6181 tcaacgtctc cgccaccgag caccccgagc tgttctgggc cctcaagggt ggcggcggca
     6241 actttggggt ggtcacgcgg ttcaccttcc gggcccacga gttcggcccg acgatgcgcc
     6301 tcggcgtgtc cctgtacgag ccggaggacg ccacccaggc gctgcgcgag tacgccgcga
     6361 tcgtcccaac cctgccccgc accgtcggct ggcacgcggc gctcaagcac gacatgccgg
     6421 ccctgccgtt cgtgccgccg gagctggtgg gccgcaggtt gctgatgctg atctcgatgt
     6481 ggctcgacga cgccgacgac ccggcgggcg ccgagctcct cgatcgcctc gtcaccgtcg
     6541 gccagccgtg cgtgaaggcc accaccgcga tgccgttcgc ggcccgcgtg cagaaggtga
     6601 tcgacgagga gttcgaggac ggccaccgct actacaccaa ggaggcccac gtctccgacc
     6661 ttgccgacga ggcgatcgac accctggtcg ccttctggaa ggacatgccg atgcacggcg
     6721 aggtcgagat catcgggctc ggcggggcga tcggcgacgt ccccgaggac gccatggcat
     6781 tctccaaccg cggctacctg ttgtggctga acttcgcgat gcgctgggac gacccgggca
     6841 acgaccagga ccacatcgag cggacccgga agatcgtcgc tgacctcaaa ccgtggaccg
     6901 ggaaaggcat ctacgtcaac atgctcaact tcgacgagat ggaccgggtc gtcgaagcct
     6961 tcggtgggcc ggagaagtac gccaggctcg gccgggtcaa ggcgcaatac gacccggaga
     7021 acctcttccg ggtgaactac aacatcacgc cggtgtcctg atgcagcccg atgtcgccct
     7081 gaccctggtc atcccgctgc gggcgaagtc ggggatgtcg ctgcccgact tctaccagta
     7141 ctggctgaac gcccacgtga cgctgccccc gcgcttcccc ggcatcagca gcatctggct
     7201 ccacgtcgtc tcgttcgacc gccagcgctg gcctcgcctg cctggagtga gccaccggcc
     7261 cgagccgcag gacgagttcc acggggtgcc cgaggcgacc ttccccacca tggccgacct
     7321 ggagctgttc cagtcctacg cccacgtcca gatggtggat ggcatcaact tcctcgggga
     7381 ggtcatcacc tacagcagcc tcggcggcaa cagcgcgacg ctggtggaca agctcggcga
     7441 cgccgccccc gacggtcacg acgacctggt gcggcacctg ctgttcctgc gccggcgcgc
     7501 cgacgtcccg gccgccgcgc tccgccggtt cgtcaccgag accctcgagc ccgcctacgc
     7561 cggctccccg gaggtcctga agctgcgg
//
LOCUS       DATBRK010000277         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2646497, whole genome shotgun sequence.
ACCESSION   DATBRK010000277 DATBRK010000000
VERSION     DATBRK010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2646497"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VIH70_08355"
     CDS             complement(<1..291)
                     /locus_tag="VIH70_08355"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY8116410.1"
                     /translation="MQAEGDVIRFPAAEQAPPDFAAFFAEEHAGLFKALFFVTGNRAD
                     AAELMQDAFLKLWERWDRIDRLDDPTGYLFRVALNGFRMRTRAAVRATRRLVP"
     gene            complement(560..1237)
                     /locus_tag="VIH70_08360"
     CDS             complement(560..1237)
                     /locus_tag="VIH70_08360"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8116411.1"
                     /translation="MVAPTGDSQAEVRTFLIADVRGWTAFTQERGDEEAARLAARLAE
                     VTRSVVEDHRGRVLELRGDEAVVVFGSPRSAIRGAVALQRRFVEETIADPSLPLTVGI
                     GLDAGEAVAVEGGYRGGALNVAARLCSLARAGEVLASREIVHLALRVEGVRFTERGQT
                     QLKGLDKPVHVVAVRSEEQDDAAAIAPFVRSTAPASTTRLRSKAMLGASASVALHRAR
                     SASGRLG"
BASE COUNT          295 a          634 c          550 g          246 t
ORIGIN      
        1 cggcaccagc ctccgcgtgg cccggaccgc ggcgcgcgtc cgcatccgga acccgttcag
       61 cgccacgcgg aacaggtacc ccgtgggatc gtcgagccgg tcgatccggt cccatcgctc
      121 ccacagcttc aggaaggcgt cctgcatcag ctcggccgcg tcggcccggt tcccggtcac
      181 gaagaacagg gccttgaaca gccccgcgtg ctcctcggca aagaatgccg cgaagtccgg
      241 cggtgcctgt tcggccgccg ggaaccggat gacgtcgcct tctgcctgca tcaccctcct
      301 agatgccgcc gacgccatga gcgcttagtc cgagacgaag aatggaccct cgagcgtccg
      361 gccgcgacct tggatccggc tcgccgtgcg gcgcgggctc ccggtgcgct ctgtgacccg
      421 gcacgaccca ccgccgcgcc gccccctcat tcgtccgagc ggagcgtgag gatggcggtg
      481 accatgtcgc gtgccgctcg aatcgcgaca tccactcgga gttcgagctc gcctgcgccg
      541 acaagcaggt cgtcgtcggc tagccgagcc gcccagaagc cgaacgagcc cggtggagcg
      601 cgacgcttgc gcttgccccc agcatcgcct tcgaacgcaa tcgagtggtg gacgcagggg
      661 ccgtcgaccg gacgaacggt gcgattgccg cggcgtcgtc ctgttcctca gaacgaaccg
      721 cgaccacgtg gaccggcttg tcgagccctt tcagttgggt ctgcccacgc tcggtgaatc
      781 ggacgccctc gacccggagc gcaaggtgga cgatctcccg gcttgccagc acctcgccgg
      841 cgcgcgcgag cgagcagagc cgtgccgcga cgttcagagc gccgccccgg tagccgccct
      901 cgaccgccac cgcctcgccg gcgtccagac cgatccccac ggtgagtggg agggacggat
      961 cggcgatcgt ctcctcgacg aagcgtcgct gcagagccac ggccccccgg atcgccgagc
     1021 gaggtgaacc gaagacgacc acggcctcat caccgcggag ctcgaggacc ctccctcggt
     1081 gatcctcgac caccgagcgc gtcacctcgg cgaggcgtgc cgccaggcgt gcggcctcct
     1141 cgtccccccg ttcctgcgtg aacgccgtcc agccgcggac gtcggcgatc aggaacgtcc
     1201 ggacctccgc ttgcgaatcg cctgtgggcg caaccacggc atctcctcca agcaacgtga
     1261 ttatccgcca ggaatggggc gtggacgact gcccgcccgg tcgcgcctac gatccgcgtc
     1321 acctctggcc gcgaacgcca cattctcgag cgcgcagttc acgccgtcac cgagcaggca
     1381 gtcaaggccg acgacctcgt ggacgaggcc atctcgaccg ggttcggccg ctccgacatg
     1441 ctcgtcgttc acgcgaagat gctccggctc gagctgccgt gggatctgaa gcacgatcca
     1501 cggaaggaag cgctcggcgg aaccaagacg gttacgaaga ggcaaggaga gatcggagag
     1561 ccgccaacgg actacacaat ttctccccgt gcacccaact ggacacccat gaggtcgggc
     1621 catgtcaagg gatcaggaat ctcgattccc acctccttct cgtagctgcc gggaacgacg
     1681 gaccgcagac ggggtcaagg tggagcgccc caccggacga acgac
//
LOCUS       DATBRK010000278         5750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2502176, whole genome shotgun sequence.
ACCESSION   DATBRK010000278 DATBRK010000000
VERSION     DATBRK010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2502176"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VIH70_08365"
     CDS             complement(<1..261)
                     /locus_tag="VIH70_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY8116412.1"
                     /translation="MSSVDHPIGSTDREIETELFTRLPEDAAARERLVEMFLPLAEYL
                     ARRFDRRGEPLEDLVQVASVGLLNAIDRFDVERDVQFSTYAAV"
     gene            complement(258..662)
                     /locus_tag="VIH70_08370"
     CDS             complement(258..662)
                     /locus_tag="VIH70_08370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8116413.1"
                     /translation="MASPRNDVIIVSVPARAGSVHVLRAVTTSVAARLPIPFDGIEDL
                     RLAVDEACAWLLARGRSATAMTLRLRPLDDRLEAEVSIDAADGPWPPPDLERSLPWRI
                     LSALVDTLSLNTDAGGPSILLTKRTLEPSRTT"
     gene            749..1084
                     /locus_tag="VIH70_08375"
     CDS             749..1084
                     /locus_tag="VIH70_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8116414.1"
                     /translation="MDVIVGTRTEEPWTVVEVAGELDLHTSPPLREHLLALIQGGADH
                     LALDLTKVDFMDSSSLGSLVTCLKRVRERDGKFVLVGVGGTPMKVLTLTGLDRVFEIV
                     GSTTELPAG"
     gene            1104..1478
                     /locus_tag="VIH70_08380"
     CDS             1104..1478
                     /locus_tag="VIH70_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8116415.1"
                     /translation="MERLSIRIPPGTAHVASVRAFVGAVGRSTGCSEEAVDDLRLIAT
                     EACAQAIEQNTPPAVIEVRSMVDGGRLLVEIEPCDRFDRAPEGLDGASGRATRRALIE
                     GLFPDAEFDDRVLRVAVPIARS"
     gene            1482..2921
                     /locus_tag="VIH70_08385"
     CDS             1482..2921
                     /locus_tag="VIH70_08385"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEY8116416.1"
                     /translation="MPRGDDTTDPMVSLERRMLDAERRAREIAENARAEAEAAREEAV
                     RAREEAEGAHRLETQARERLQVLVEAGVAMAASRETRSVLAAATGAAARRACDYSLAF
                     VVDRDGRVEAAVGAHRDPGRFGMVERMANAHLPDPGDPDSIIAEVLKTGEAALIPNLS
                     SERVEQMMAPGEQLELARSLGLASLIAAPVAARGRTLGVLVLVRDDPSTPFGEEDLSL
                     AGMLAARAGLAVDNVRLYAEREYVAETLRRSLLPPELPGIEGLEIAARYLPAAEGIQV
                     GGDFYDVFEAAPERWIAVIGDVVGKGAEAAAMMGLARYTIRTAAMSEGQPSAILETLN
                     QAVLNQTDDERFCTACCVRLRPWGSQTRAIVSSGGHPLPLVLRADGSIEVVGSPGTIV
                     GVFEDPVLVDRAVDLGLGDALVLYTDGVTDERSDEDEFGETRLHEVLSELKGAGAQRI
                     ADGVVEAVVGFRSGQPKDDIAILAIKVVE"
     gene            3116..3373
                     /locus_tag="VIH70_08390"
     CDS             3116..3373
                     /locus_tag="VIH70_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116417.1"
                     /translation="MTDLLAPTPVKWLYCFACEETFELAGSDASCGCGRSSARLDGGI
                     VEVQGPTRALAPVETVIRLDGGEWAPLPEDVFIRRVLPRAA"
     gene            complement(3436..4170)
                     /locus_tag="VIH70_08395"
     CDS             complement(3436..4170)
                     /locus_tag="VIH70_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY8116418.1"
                     /translation="MVVSVGTSGWQYRDWRDGFYPAGLAAADWLAFYAQRFSTVEVNA
                     SFYRLPKRETVERWAATVPDGFVLTFKASRYVTHVRRLRDCAEPLERMRGVFEGAGTK
                     LGPVLFQLPPTLRADEPLLRDFLASLPPSFRAAIEFRHPSWFTDGVLEALDASTAALV
                     HADRPGVRVSIPTLGGWAYLRFHQGRSDGPGYRRSKLAAYADAIAETRPRSAFAYFNN
                     DTDGAAPRDAAAFAALLADRHVHVAA"
     gene            complement(4217..5134)
                     /locus_tag="VIH70_08400"
     CDS             complement(4217..5134)
                     /locus_tag="VIH70_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116419.1"
                     /translation="MSRRARTAWRIGAFVALVFLMTGCIKLNMNLGINSDNTVSGTVE
                     FGVQKELLDLTGQNVEDLLGTDSPFASNAPGVTVEPFDDGEFAGQQFIFEDLPIEEFN
                     SGGVAGASGATGVTGAGDSLQIARQGDTFVVTGLLDLSSGLSGVTGPFGGTGGAQFLE
                     SADIKIAITFPGEVLEAPGGQIDGNTVTYLPEFGERLEIDATGSAVDNGEAADVVGGS
                     DSFLPLLLIIAGVVLVLLLVILLVVRSRRNKVGEGTPSGFVEAGSAAHAVAAPPGAPA
                     DAPPPGAPTEPPPPAPPMPPAPPPPPAEG"
     gene            complement(5200..5670)
                     /locus_tag="VIH70_08405"
     CDS             complement(5200..5670)
                     /locus_tag="VIH70_08405"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEY8116420.1"
                     /translation="MRGAPVKRSRRGGFDLRIPTAEREVLGTIPDMLRALLVDGDAAD
                     PALRRLYPKAFTDDEDASAEFHDITHDELLEQRLAAIDTMERTLRSDHVTEDELLAWL
                     AALNDVRLVLGVRLAVTEEATAADFAGDPEAGRAFELYRYLSYLEEHVVDALSS"
BASE COUNT          888 a         1992 c         2074 g          796 t
ORIGIN      
        1 gacggcggcg tacgtggaga actggacgtc gcgctccacg tcgaagcggt cgatcgcgtt
       61 caggagtccg acgctcgcga cctgcacgag gtcctccagc ggctcgcccc gtcggtcgaa
      121 ccgacgggcg aggtattccg caagcggaag gaacatctcg accagccgct cgcgggccgc
      181 ggcgtcctcg ggcaggcgcg tgaacagctc ggtctcaatc tcacggtcgg tggaaccgat
      241 cggatggtcg accgagctca tgtcgtccgc gatggctcga gcgttcgctt ggtcaggagg
      301 atcgacggac cacccgcgtc cgtgttcagc gagagggtgt cgacgagcgc cgaaaggatc
      361 ctccacggga ggctccgctc caggtcgggt gggggccacg ggccatccgc ggcatcgatc
      421 gagacctccg cctccaggcg atcgtccaga ggccggagcc gaagggtcat cgcggtggcc
      481 gatcgaccgc gagcgagtaa ccacgcgcac gcctcgtcga cggcgaggcg gagatcctcg
      541 atcccgtcga acgggatcgg cagccgagcg gccaccgacg tcgtgacggc gcgcaggacg
      601 tggacggatc cggccctcgc cgggacggac acgatgatga catcgttcct cgggctcgcc
      661 atcgcgctcc ctatactagg gaagctcggg cacggcccgg acgacctacg gggtacgtgg
      721 agccgagcct cgaccgagag ggcaacggat ggatgtcatc gtcgggacgc gcaccgaaga
      781 accgtggacc gtcgtcgagg tcgccggcga gctcgacctc cacacgtcac cgccgctccg
      841 cgagcacctg ctggcgctga tccagggggg tgccgaccac ctggccctgg acctcacgaa
      901 ggtcgacttc atggactcga gcagcctggg gtcgctggtg acgtgcctca agcgcgtccg
      961 ggaacgcgac ggaaagttcg tccttgtcgg cgtcggcggc accccgatga aggtgctcac
     1021 cctcacgggg ttggaccgcg tgttcgagat cgtcggctcg accaccgagc tccccgcggg
     1081 ctgacgcggg aggtcgaagc ccgatggaac gcctctcgat ccgcatccca cccgggacag
     1141 cgcacgtggc gtcggtccgc gccttcgtcg gagcggtcgg tcgcagcacc ggctgctcgg
     1201 aggaggcggt cgacgacctt cggctcatcg cgaccgaggc ctgcgcccag gcgatcgagc
     1261 agaacacgcc gccggccgtg atcgaggtcc gctcgatggt cgacgggggc cgcctgctcg
     1321 tggagatcga gccctgcgac cggttcgacc gagccccgga gggactcgac ggggcctcgg
     1381 gacgggcgac gcgtcgagcg ctgatcgagg gcctgttccc cgacgccgag ttcgacgacc
     1441 gggtcctgcg cgtagccgtg ccgatcgcgc ggtcgtaggc cgtgccacgg ggtgacgaca
     1501 cgaccgaccc gatggtctcg ctcgagcggc ggatgctcga cgccgagcga cgcgcacgcg
     1561 agatcgccga gaacgcgcgg gccgaggccg aggccgcgcg cgaggaggcg gtgcgcgccc
     1621 gcgaggaagc cgaaggcgcc caccggctcg agacccaggc gcgcgagcgg ctccaggtcc
     1681 tcgtcgaggc cggcgtggcc atggccgcct cgcgcgaaac gcgctcggtc ctggccgcgg
     1741 cgaccggggc ggcggcccgc cgggcctgcg actacagctt ggccttcgtg gtcgatcggg
     1801 acggccgcgt ggaggcggcc gtcggagcgc accgagaccc ggggcggttc gggatggtcg
     1861 agcggatggc gaacgcccac ctgcccgacc ccggggatcc cgacagcatc atcgcagagg
     1921 tcctgaagac cggcgaggcg gcgctgatcc cgaacctctc atccgaacgg gtggaacaga
     1981 tgatggcgcc cggggagcag ctcgagctcg cgcgctccct gggtctcgcg tcgctgatcg
     2041 cggcgccggt cgcggcccgc ggacggacgc tcggggtgct cgtgctcgtg cgtgacgacc
     2101 cgtcgacacc gttcggggag gaggacctgt cgctcgcggg gatgctcgcg gcccgggccg
     2161 gcctcgccgt cgacaacgtc aggctctacg ccgagcgcga gtacgtggcg gagacactgc
     2221 ggcggagcct cctgccgccg gaactgccgg ggatagaggg tctggagatc gccgcgcgct
     2281 acctccccgc cgcggaggga atccaggtcg gcggcgattt ctacgatgtc ttcgaggcag
     2341 cgccggaacg ctggatcgcg gtgatcggcg acgtggtcgg caagggggcc gaggccgcgg
     2401 cgatgatggg gctcgcgcgc tacacgatca gaaccgccgc gatgagcgag ggccaaccga
     2461 gcgcgatcct ggagacgctc aaccaggccg tcctgaacca aacggacgat gagcggttct
     2521 gcaccgcgtg ctgcgtgcgc ctgcgaccgt gggggagcca gacgcgggcg atcgtgtcgt
     2581 ccgggggcca tccgctcccc ctggtccttc gcgcggacgg ctccatcgag gtcgtgggca
     2641 gtccggggac catcgtcggg gtgttcgagg acccggtgct ggtcgaccga gccgtggatc
     2701 tcggcctcgg cgacgcgctc gtcctgtata ccgacggcgt caccgacgag cgaagcgacg
     2761 aggacgagtt cggtgagacc cggctgcacg aggtgctgtc cgagctcaag ggcgccggcg
     2821 cgcaacggat cgccgacggc gtcgtggagg cggtggtcgg ctttcggagc gggcagccga
     2881 aggacgacat cgcgatcctc gcgatcaagg tggtcgagtg acgacactcg acgtcgatcc
     2941 ggtcccacca catgcacgtg ggtgtttgag gggccgaacg cccgggtacc caaaaccctg
     3001 atgcgatcaa cgaccagcgg ccaacatgcc ggtggtccga gaggattgac ggagcggtcc
     3061 ggcgcgcccc ctcccccctg ctcgtcggac cgctcgtcaa cccgaggtga tggccatgac
     3121 cgacctgctg gcaccgacgc ccgtgaagtg gctgtactgc ttcgcgtgtg aggagacgtt
     3181 cgagctcgcc gggtccgacg cgtcgtgcgg atgcggacgc tcgtccgcgc ggctggacgg
     3241 cgggatcgtg gaggtccagg gcccgacgcg cgcgctcgcg ccggtcgaga cggtgatccg
     3301 gctcgacggc ggcgagtggg ctccgctccc cgaggacgtc ttcatccgtc gggtgctgcc
     3361 aagggccgcc tgacgcgctc ccgggatccg catcctcctc caccacggat cccgaaacct
     3421 ccatcaagct cggtcctacg cggccacgtg gacgtgtcga tcggcgagca gtgctgcgaa
     3481 cgcggcggca tcccgcgggg ccgcaccgtc ggtgtcgttg ttgaagtacg cgaaggccga
     3541 tcgcggccgc gtttcggcga tcgcgtccgc gtacgcggcg agcttggagc gtcgatagcc
     3601 gggcccatcc gaacgtccct ggtggaaccg tagataggcc catcccccca acgtcgggat
     3661 cgacacgcga acccccggac ggtcggcgtg gacgagcgcc gccgtgctcg catcgagcgc
     3721 ctcgaggacc ccgtccgtga accacgacgg gtgacggaac tcgatcgccg cccggaacga
     3781 cggcgggagc gacgcgagga agtcccggag cagcggctcg tcggcccgaa gggtcggcgg
     3841 tagttggaag agcacgggcc cgagcttggt gccggcgccc tcgaagaccc ctcgcatccg
     3901 ctcgagcggc tccgcgcagt ctcgcagcct gcggacgtgc gtcacgtacc ggctcgcctt
     3961 aaaggtcaga acgaagccgt cgggcacggt cgcggcccat cgttcgaccg tctcacgctt
     4021 cggcaggcga tagaacgacg cgttgacctc cacggtcgag aagcgctgag cgtagaacgc
     4081 cagccagtcg gctgcagcca gccccgcagg gtagaagcca tctcgccagt cccggtactg
     4141 ccacccactc gtaccgacgg ataccaccac gcggggtggc taccctcgcg gggcacccgt
     4201 cacacgcttg aggacgtcag ccctcggcgg gcggcggcgg cggggccggc ggcatcggtg
     4261 gcgccggtgg aggcggctcg gtcggtgctc ccggtggcgg cgcatcggcg ggtgctcccg
     4321 gcggcgccgc gacggcgtgc gccgcggaac ccgcctcgac gaaccccgac ggcgtcccct
     4381 cgccgacctt gttccggcga ctccgaacca cgaggaggat gacgagcagg agcacgagga
     4441 cgacccccgc gatgatgagg agcagcggca ggaacgaatc gctgccaccc acgacgtccg
     4501 cggcctcgcc gttgtccacg gcgctgccgg tcgcgtcgat ctccaaccgc tcgccgaact
     4561 ccggcaggta ggtgaccgtg ttgccgtcga tctgtccacc cggggcctcg agcacctcgc
     4621 cggggaacgt gatcgcgatc ttgatgtccg cgctctcgag gaactgcgcg ccgccggtgc
     4681 cgccgaaggg cccggtgacg ccggacagac cgctcgacag gtcgaggagg ccggtgacca
     4741 cgaacgtgtc cccctggcgt gcgatctgca gcgagtcccc cgcaccggtc acgcccgtgg
     4801 ctcccgaggc acccgcgacc ccgcccgagt tgaactcctc gatcgggagg tcctcgaaga
     4861 tgaactgctg gcctgcgaac tcaccgtcgt cgaagggctc caccgtgacc ccgggggcgt
     4921 tgctcgcgaa cggggaatcg gtcccgagca ggtcctcgac gttctgcccg gtcagatcga
     4981 gcagctcctt ctggacgccg aactcgacgg tcccgctcac cgtgttgtcg gagttgatcc
     5041 cgagattcat gttgagcttg atgcaccccg tcatcaggaa gaccagcgcg acgaacgcgc
     5101 cgatccgcca cgcggtgcgg gcacgtcgag acatccctgg accccctgtc gtcggcatcc
     5161 cggggagact acctcgggcg cacgggcggg cgagcgatct caggaggaga gggcgtccac
     5221 gacgtgctcc tcgagatagg agaggtagcg gtacagctcg aaggcgcgcc cggcctcggg
     5281 atcgccggcg aagtccgcgg ccgtcgcctc ctcggtgacg gccagccgga caccgagcac
     5341 cagccggacg tcgttcaggg cggcgagcca cgcgagcagc tcgtcctcgg tgacgtggtc
     5401 ggatcgcagg gtccgctcca tcgtgtcgat cgccgccagc cgctgctcga gcagctcgtc
     5461 gtgtgtgatg tcgtggaact cggcggacgc gtcctcgtcg tcggtgaagg ccttggggta
     5521 caaccgccgc agcgccggat cggcggcatc gccgtcgacc agcagcgcac gcagcatgtc
     5581 cgggatcgtg ccgaggacct cgcgctccgc cgtcgggatc cgaagatcga acccgcctcg
     5641 gcgcgaccgc ttgaccggcg ctccccgcac gctcagcggt ccttctgcaa cgtggcccac
     5701 agcccgtgcg cgtgcaggcg cgacacgtcg aactcacatc gctcgcgggg
//
LOCUS       DATBRK010000279         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_481442, whole genome shotgun sequence.
ACCESSION   DATBRK010000279 DATBRK010000000
VERSION     DATBRK010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481442"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..696
                     /locus_tag="VIH70_08410"
     CDS             250..696
                     /locus_tag="VIH70_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018809209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8116421.1"
                     /translation="MTKQTYDLVAEHLLDATDEDVFDAYTDAGGPDWDVDVGCDLRVG
                     GVWSIRSAPPDGTAYRETNRFTMVDRPRRLAFESALTMPDGSTVRRDVDVTFRNEERG
                     RTRMTIVQKGFPTAESRDAFGAGFPGIFERLEHLAARSSSRGRARS"
     gene            696..1424
                     /locus_tag="VIH70_08415"
     CDS             696..1424
                     /locus_tag="VIH70_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006107449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8116422.1"
                     /translation="MTSLENKHAVVYGAGGTVGQAVARAFASGGARVSLAGRTRATLD
                     GLARDIRGAGGDVADIAQVDALDASALERHIGGLVDEFGGIDICFNAVGDEATLGPSL
                     IELGFPDFVNPITRLVSAQFLIATAVARQMIERKSGVILTMTGSGVPTAGMGGAMTAW
                     AAVDALCGQLARELGPFGIRVLWLRSNGVSASGGDPAERERSMLHRLASPDDVGKVAS
                     FLASDQAVTMTATAANITSGAEVG"
     gene            1572..2222
                     /locus_tag="VIH70_08420"
     CDS             1572..2222
                     /locus_tag="VIH70_08420"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY8116423.1"
                     /translation="MRLVDLSHVIRHGMVTYPGLPGPEIGEHLSWEASRERYAPGTEI
                     RIAQISMVANTGTYLDTPAHRYRDGWGIDDLPLEMVAAVPGIVVDDTGPEIGPDALKG
                     LEVAQRAVLVRTGWSRHWGTERYGEQGHPYLTAAAAEALVAGGATLVGIDSVNIDGTH
                     TGERPVHTILLGAGIPIIEHLTSLETLPAEGFDFFAVPQRVEGMGTFPVRAFAMVP"
BASE COUNT          381 a          675 c          793 g          385 t
ORIGIN      
        1 aatactggcc tgagggacga ggaggccagg agaacgcatg aagaaggctg gcaggcgtcc
       61 ttcgacaatc tcgaccgcgt cctggcgtcg tgaactctcc gatccccagc cgttttccgt
      121 gcgctcgtta ccccgacgac gttgccgcta gcaccgagat ctcggggcag ggacttcgcg
      181 cgacggccgc gactggaagc cgggtgagcc cttgccgggt cccctcggcc agcgagagga
      241 ggtagaccga tgaccaaaca gacctacgac ctcgtcgcgg agcatcttct cgatgcgacc
      301 gacgaggatg tcttcgacgc gtacacggat gccggcgggc cggactggga cgtcgacgtt
      361 gggtgtgacc tccgtgtcgg aggcgtgtgg agcatccgaa gtgcaccacc ggatgggacg
      421 gcgtaccgcg agacgaaccg gttcaccatg gtcgatcgcc cccgccgctt ggccttcgag
      481 tcggcgttga cgatgccgga tggctccacc gtccgccgcg acgtagacgt cacattcagg
      541 aacgaggagc gtgggcggac gcgaatgacc atcgtgcaga agggttttcc caccgcggag
      601 tcacgggacg cattcggagc gggctttccc ggcatctttg aacggctcga gcacctggcg
      661 gcaaggtcgt cgtcgcgagg aagggcgagg tcctgatgac gtcattggag aacaagcatg
      721 ccgtggtcta cggcgcggga ggaaccgtgg gtcaagcggt ggcacgcgcc tttgcttctg
      781 gtggagcgag ggtgtctctg gccggtcgga cgcgtgcgac gttggacggg ctcgcccgcg
      841 atatccgtgg cgcgggcggc gacgtcgccg acattgcaca ggtcgacgcg ctggatgcgt
      901 cggctctcga gcgacacatc ggagggttgg tcgatgagtt tggcggcatc gacatctgtt
      961 tcaacgcggt gggggatgag gcgaccctcg gtccgtccct gatcgagctg gggttcccgg
     1021 acttcgtcaa cccgatcacc cgcctcgtga gcgcacagtt cctgatcgcg acggcggtcg
     1081 cacggcagat gatcgagcga aagtcgggcg tcatcctgac catgaccgga tcgggcgtgc
     1141 caacggccgg catgggcggt gccatgacgg catgggccgc cgtcgacgcc ctctgcggcc
     1201 agcttgcgcg cgaactcgga ccattcggga tccgtgtgtt gtggctccgc tcaaacggcg
     1261 tcagtgcgag cggaggcgac cccgccgagc gtgagcggag catgctccac cggctggcat
     1321 cgcctgatga cgtcggcaag gtggcgtcct tcttggcctc ggaccaggcc gtaacgatga
     1381 ccgcgaccgc cgccaacatc accagcggcg ccgaggtcgg ctaggcccgc ctgtcaggtc
     1441 gttgctatcg actggaggtg tttgaggaga gccacggtgg ggtggggatc ggatcgcgat
     1501 gttcgtggac ggtttcatag ccgaaccaaa cgagaaggtc gcggggactt ggccaaggag
     1561 gcgagcggtc gatgcggttg gttgatctca gtcacgtgat ccgccatggg atggtgacgt
     1621 accccggact cccgggaccc gagatcgggg agcatctgag ctgggaggcc tccagggagc
     1681 gttatgcgcc agggacggag atccgcatcg cccagatctc gatggtggca aacaccggga
     1741 cctatctcga caccccggcg catcgttacc gtgatgggtg gggaatcgac gaccttcccc
     1801 tggagatggt tgcggcggtc cccgggattg tcgtcgacga cacgggtccc gagattggcc
     1861 cggacgccct caaaggactc gaggtcgcac agcgggcagt tctcgtgcgg accggctggt
     1921 cacgtcactg ggggaccgaa cgctacggtg agcagggcca cccctacctg accgccgcgg
     1981 cagcggaggc cctggttgcg ggtggagcaa cgctggtcgg gatcgattcg gtgaacatcg
     2041 acggaacgca taccggagag cggcccgtgc acacgatcct gcttggggcc gggatcccga
     2101 tcatcgagca cctcacgagc ctcgaaacgc ttcctgcgga ggggttcgat ttcttcgccg
     2161 ttccgcagcg ggttgagggg atgggcacct tccccgttcg ggctttcgcg atggtgccat
     2221 gaccttaacc tgac
//
LOCUS       DATBRK010000280         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2210458, whole genome shotgun sequence.
ACCESSION   DATBRK010000280 DATBRK010000000
VERSION     DATBRK010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210458"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="VIH70_08425"
     CDS             <1..125
                     /locus_tag="VIH70_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007532486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY8116424.1"
                     /translation="LASLIAGDGLRFRPSGLIGSILGAIIVTAVWGLVRGRRTA"
     gene            304..723
                     /locus_tag="VIH70_08430"
     CDS             304..723
                     /locus_tag="VIH70_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8116425.1"
                     /translation="MQAGTFATVPAVSALALVAGRRRLAASLAVGGTIAWVGAKLAKP
                     FGGRARPRGVLPDVRIREGIEGDLGWVSGHTAVATTLAFVAAQELPRTARPLLAGIVG
                     TVGFGRMYVGAHLPHDVVGGAGLGMMISSVIPPTDAS"
     gene            complement(725..973)
                     /locus_tag="VIH70_08435"
     CDS             complement(725..973)
                     /locus_tag="VIH70_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY8116426.1"
                     /translation="MTWTSILPGALPVGVGVRLLGLAVSTYFAYRLDHSNDLYGALGL
                     AVVMMLFLFLIARLFVAAQFLTSTLHRRREPLPSAGGV"
     gene            complement(970..1176)
                     /locus_tag="VIH70_08440"
     CDS             complement(970..1176)
                     /locus_tag="VIH70_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116427.1"
                     /translation="MWHPRSRASALKGSPAVTGFGLLAVALQAFLAKISQTLGLPVLV
                     RFVLGLILASSLAFACCGCCRART"
     gene            1367..>1954
                     /locus_tag="VIH70_08445"
     CDS             1367..>1954
                     /locus_tag="VIH70_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116428.1"
                     /translation="MDEGAASPERLGRVVPLRVAAQPPPPLRELVEQSFRIAIGATAL
                     ASEVLAEAVARTLGREPFAEGETPGDETARPASGVPLVAGAAIGVALEGARWGVRAAT
                     TMVSSAELFLSLLTSPGFVQEPLRQAGERLGALDARWREQRPRDEEAAASFLRLVVPQ
                     VVDAALDQIDLNELVAERIELDQVIDRLDLDRAIAR"
BASE COUNT          278 a          716 c          715 g          245 t
ORIGIN      
        1 tgctcgcgag cctgatcgcg ggtgacggcc tgaggtttcg gccgagcggc ctgatcgggt
       61 cgatcctcgg cgcgatcatc gtgaccgccg tgtgggggct ggtgcgcggt cggcgcaccg
      121 cgtaacgctc ggccgcgacg caccgtccgg acccggcggc tcggtcggtt cgcgctcggg
      181 gcggccctgt tcctcgtcag cctcgccgag gcccggcgag gcaggctccc cgactacgag
      241 gaatgcgtct tccggaccgt caacggctcg agcgaccgga tccgcgtccc cgttcgagcg
      301 atcatgcagg ccgggacgtt cgcgacggtc ccggcggtct cggccctcgc gctcgtggcc
      361 ggccgcaggc gccttgccgc gagcctggcc gtgggcggca cgatcgcctg ggtgggcgcc
      421 aagctcgcca agccgttcgg gggccgggcg cgaccgcggg gcgtcctgcc cgacgtcagg
      481 atccgcgagg ggatcgaggg cgacctcggg tgggtctcgg gccacaccgc cgtcgcgacc
      541 acgctcgcgt tcgtcgcggc acaggagctc cctcggaccg cgcgacccct cctcgcgggg
      601 atcgtgggga cggtggggtt cggccggatg tacgtcggcg cgcatctgcc ccacgacgtc
      661 gtcggcggcg ccgggctggg gatgatgatc tcctccgtga tcccgccgac cgacgcgagc
      721 tgagtcaaac cccgcccgcg ctcggcagcg gttcacgccg ccgatgcagg gtcgaggtca
      781 ggaactgcgc cgccacgaag agccgcgcga tgaggaacag gaacagcatc atcacgacgg
      841 cgagcccgag cgccccgtac agatcgttgc tgtggtcgag gcggtaggcg aagtacgtcg
      901 acacggccag ccccagcagc cgcaccccga cacccaccgg cagggcaccc ggaaggatcg
      961 aggtccaggt cacgtccgcg ctcggcagca accgcagcac gcgaacgcca acgaggaggc
     1021 caggatcaga ccgaggacga accgtacgag gacggggagc ccgagcgtct ggctgatctt
     1081 cgccaggaac gcctgcaggg cgacggcgag cagaccgaag ccggtgacgg ccggggaacc
     1141 cttcagcgcg ctcgcgcggg agcgcggatg ccacacgcaa gctcgctcac gaaccgaaac
     1201 ccccggatcc cggggcgggc ggctgcgtct ccagacgtcc tccacgcgtc ggcgcgtgat
     1261 cggcccggac ggttcgggca ccggcggtgg tagcccagcg acgacgcact gttggcacgt
     1321 cgtcgccccc ggggtgatga tgggcacccc gaacgagagg ccgaccatgg acgagggcgc
     1381 cgcttcaccc gaacgtctcg gcagggtcgt cccgctccgg gtcgccgccc agcccccgcc
     1441 gccgctccga gagctcgtcg agcaatcgtt ccggatcgcg atcggggcga cggcgctcgc
     1501 ctccgaggtc ctcgccgagg ccgttgcgcg aacgctcggc cgcgagccgt tcgccgaggg
     1561 cgagacgccc ggcgacgaga ccgctcgacc ggcctccggg gtcccgctcg tcgcgggcgc
     1621 ggcgatcggc gtcgccctcg agggcgcccg gtggggcgta cgcgccgcca ccacgatggt
     1681 cagcagcgcc gagctgttcc tctcgctcct gacgagcccg gggttcgtcc aggaaccact
     1741 ccgccaggcc ggggagcggc tcggggctct cgacgcccgc tggcgtgagc aacgcccgcg
     1801 cgacgaggag gcagccgcat cgttcctgcg gctcgtggtc ccccaggtcg tggacgcggc
     1861 gctcgaccag atcgacctca acgagctcgt cgccgaacgc atcgagctcg accaggtgat
     1921 cgaccggctc gacctcgacc gggcgatcgc gcga
//
LOCUS       DATBRK010000281         5517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2357839, whole genome shotgun sequence.
ACCESSION   DATBRK010000281 DATBRK010000000
VERSION     DATBRK010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2357839"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..277
                     /locus_tag="VIH70_08450"
     CDS             5..277
                     /locus_tag="VIH70_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8116429.1"
                     /translation="MVCFFVDSKARGRGLSQRMLEAAVDYARSRGARLVEAYPVDKDQ
                     RSHPDDMFFGAKSMYDRAGFREVARRRPTRPVVRRSLRPRRAAQRR"
     gene            complement(284..1033)
                     /locus_tag="VIH70_08455"
     CDS             complement(284..1033)
                     /locus_tag="VIH70_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8116430.1"
                     /translation="MGVRGADMQIASGIHRIGDRSIINAYLIEEANEVTLVDAGVAGL
                     YRDLPKELASMGRTIEDVRALVLTHGHSDHIGFAERLRTERHVPVSVYEADAALARGE
                     VPNPSKGFGPTKVGPLVGFLWFSLLRGGLRTRHLGEVSTFGDGATLDVPGSPVVTLTP
                     GHTPGCAVLHFRSLGALVIGDAFATYAVTTGARGPRIAPFTADAARAVESLTRIEDVS
                     SDLVLPGHGDPWTGGVAEAVRLVRESAASKG"
     gene            1196..2107
                     /locus_tag="VIH70_08460"
     CDS             1196..2107
                     /locus_tag="VIH70_08460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8116431.1"
                     /translation="MEQRRLGRLGHMSSVLIYGGAALWDVTQEVADRSIRLALDSGIN
                     HFDTAADYGDSELRLGTWMPRIRDRIFLATKTGDPTATEAYDSIRRSLERLQVMRVDL
                     VQLHSVGAFDDLDRRTASGGALEGAIRARDEGLVAAIGVTGHGMKAPAVHLEALRRFR
                     FDTVLTPYNYRLTRYPEFLHDFEALVEEVRAQDAGLMLIKAVARNLWRTGERHRFATW
                     YEPLDERSTIDAAMAFALGRREATGVCTAGDVRLVPKQIEALERIASIPIDEAAATLE
                     RVPDLEPPFVRTEGRVVPEWLEPLLDR"
     gene            2118..3122
                     /gene="ligD"
                     /locus_tag="VIH70_08465"
     CDS             2118..3122
                     /gene="ligD"
                     /locus_tag="VIH70_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY8116432.1"
                     /translation="MNPILGELDARDRRLLRRRAMPAFTPLMLATLAKDPFSDPAWVF
                     EAKLDGQRSLLWRRGPTVRLITRNEKDRTSHYPDLVAAILRDDTAPLIADGEIVAFEG
                     DLTSFSRLQERMQNARPSAAQIAAVPVVFYLFDLIWFDGYDLSALPLVARKSVLHGAL
                     AFADPIRFSEHLDEEGEVAFRAACEKGWEGLIAKRASAPYTHGRSKEWLKFKCVNEQE
                     FVVLGWTDPHGSRSGLGALLVGYHEDGALRFGGKVGTGFNERELTRLTAILRPLEQTD
                     PPVGDVKDLSTKGVHWVRPELVAQVGFSEWTPDGKLRHPRYLGLRDDKRPKQVVRERA
                     "
     gene            complement(3138..3593)
                     /locus_tag="VIH70_08470"
     CDS             complement(3138..3593)
                     /locus_tag="VIH70_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8116433.1"
                     /translation="MDGKLDWLAAGLPTEGTNAQLPRAGTVARRDVPTCRLDELLGPV
                     RQRVREAGWDACVVVNDERVVFGLLRHAQLERDGDESIERVMRPGPSTFRPYVPIAEM
                     AAFMVSHDLPSSPITTSDGLLVGLIRREDAVQAAHELHAGNEQTHEHGG"
     gene            complement(3657..3863)
                     /locus_tag="VIH70_08475"
     CDS             complement(3657..3863)
                     /locus_tag="VIH70_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8116434.1"
                     /translation="MPHEIHERREVQALMERGAQIVEVLPRDEFEEDHLPGAIHLPLR
                     KIVAEARQRLDGDRQIVVYCWDSA"
     gene            complement(3911..4180)
                     /locus_tag="VIH70_08480"
     CDS             complement(3911..4180)
                     /locus_tag="VIH70_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY8116435.1"
                     /translation="MIRPQRNRRHDPERDSAEYVSGELSVRARRWFERHLLDCDDCWR
                     EVLLGRFGRRIAEDAREPAPVGLRDRVRAAVVLSSGGGLPSEPSG"
     gene            complement(4177..4701)
                     /locus_tag="VIH70_08485"
     CDS             complement(4177..4701)
                     /locus_tag="VIH70_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8116436.1"
                     /translation="MRDDHEEFLRATMGAMDLVYNLARRFAVRPEDAEDLVQETYLAA
                     YRAWRDRRRPRRVEPWLATICLNLGRSRHRSRGRRPIEVSIEDRDFRAPATSDPERAA
                     EAALDREALYEAMRRLPEEQRVAITLVDLVGLKTAEAAKAMGVPRGTVLSRLHRGRRG
                     LAALLWDEVRGGTR"
     gene            complement(4711..4851)
                     /locus_tag="VIH70_08490"
     CDS             complement(4711..4851)
                     /locus_tag="VIH70_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116437.1"
                     /translation="MEGQEQNGGHICAKCGASFETQEELDRHVASAHGDGGEEAGSKP
                     DK"
     gene            complement(4871..5458)
                     /locus_tag="VIH70_08495"
     CDS             complement(4871..5458)
                     /locus_tag="VIH70_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116438.1"
                     /translation="MATAGSISRSASVSLSHVLTGALVGVVAAIVMAMFAMVAAATYQ
                     GTGFFAPLYHIASSLIDPATMMRSMEAAANGDAFTFSAGPAAVGVALHLLTGAFWGAI
                     FGLIVSTGKLNGWAGLLAGIVYGLAVMLVMSFVALPVIADVFGGGDPISEMPRLAGWG
                     TFTVEHALYGFVLGLWPLFKGSEARARRSVRQAAA"
BASE COUNT          849 a         1951 c         1878 g          839 t
ORIGIN      
        1 gtccatcgtc tgcttcttcg tcgacagcaa ggctcgaggt cgaggcctat cacaacggat
       61 gctcgaggcc gccgtggact atgcgcggtc tcgcggcgcc cgcctcgtgg aggcgtatcc
      121 ggtggacaag gaccaacgca gtcaccccga tgacatgttc ttcggcgcga agtcaatgta
      181 cgaccgcgcc ggattccggg aagtggcccg acgcaggccc acccggcccg tggtgcgcag
      241 gtcgcttcgg ccgcggcgcg ccgctcaacg tcgctgatcg ccgtcagcct ttcgacgcgg
      301 ctgactcccg caccagccga acggcttccg cgacgcctcc cgtccaggga tcgccgtgtc
      361 cggggagcac gaggtccgag gacacgtcct cgatccgagt cagcgactcg acggcccgcg
      421 cggcgtctgc ggtgaacggg gcgatgcgcg ggccccgagc ccccgtcgtc accgcgtagg
      481 tcgcgaacgc atcgccgatc acgagcgcgc cgagcgaccg gaagtggagc acggcacagc
      541 ccggggtgtg gcccggcgtg agggtgacga ccggactccc gggcacgtcg agggtcgcgc
      601 cgtccccgaa cgtcgagacc tcgccgagat gtcgggtccg aaggccgcct cgcagcagcg
      661 agaaccagag gaacccgacc agtggtccga ccttggtcgg accgaacccc ttcgaggggt
      721 tcggcacctc gccgcgggcg agcgccgcgt cggcctcgta taccgatacc gggacgtgtc
      781 gctcggtgcg gaggcgctcg gcgaagccga tgtggtcgct gtggccgtgg gtcagcacca
      841 gggcgcgcac gtcctcgatc gttcggccca tcgatgcgag ctccttcggg aggtcgcggt
      901 acaggccggc cacccctgcg tccacgaggg tgacctcgtt cgcctcctcg atgaggtagg
      961 cattgatgat gctgcggtct ccgatgcggt ggatgcccga tgcgatctgc atatcggctc
     1021 cccgcacccc catcgcccga cctcgaggtt cgtacgacgt caccccgctc gccgccgggc
     1081 agcgccgacc ggagcctacg agagttcggc aggatcgcct tctcgccctg caggagtctg
     1141 cttgccaggg ttctcttcga tagggtggaa cggatcgacc ggaggttcgg cggcgatgga
     1201 gcagcgaaga ctcgggcgcc tgggccacat gagcagcgtg ctgatctacg gcggggcggc
     1261 cctctgggac gtcacgcagg aggtcgccga tcgctcgatc cggctcgcgc tggattcggg
     1321 catcaaccac ttcgacacgg cggcggacta cggcgactcg gagctgagac tcggaacgtg
     1381 gatgccgagg atccgcgatc ggatcttcct cgcgaccaag acgggagacc cgacggcaac
     1441 cgaggcatac gacagcatcc gccgctcgct cgaacggctg caagtgatgc gggtggacct
     1501 ggttcagctc cactccgtcg gggccttcga cgacctcgac cggcggacgg cgtccggcgg
     1561 agctctcgag ggtgcgatcc gcgcacgtga tgaagggctc gtcgccgcga tcggcgtcac
     1621 gggacacggg atgaaggcgc ccgcggtgca tctggaggcg ctgcggagat tccgtttcga
     1681 cacggtcctc accccgtaca actaccgcct cactcggtac ccggagttcc tgcacgactt
     1741 cgaggcgctc gtcgaggagg tccgcgcgca ggatgcgggc ctcatgttga tcaaggcggt
     1801 cgcccgcaac ctctggagga cgggggaacg gcaccggttc gccacgtggt acgagccgct
     1861 cgatgagcgg tcgacgatcg acgcggcgat ggccttcgcc ctgggtcggc gggaggcgac
     1921 gggcgtgtgc accgcgggtg acgtgcgtct cgttccgaag cagatcgagg cgctggaacg
     1981 catagcctcc atcccgatcg atgaggccgc cgccaccctc gagcgggtgc ccgacctcga
     2041 accgccgttc gtgcggaccg aggggcgggt cgtgccggag tggctcgagc ccttgctgga
     2101 tcgctgactt ccgtcgcatg aaccccatcc tcggcgaact cgatgcccgc gatcgtcgcc
     2161 tgctccggcg tcgcgcgatg ccggccttca cgccgctcat gctggccaca ctggccaagg
     2221 acccgttctc cgaccccgcg tgggtgttcg aggccaagct ggatgggcag cgctcgctgc
     2281 tgtggaggcg tggtccgacc gtccggttga tcacgaggaa cgagaaggac cgcacgtccc
     2341 actacccgga tctcgtcgca gccatcctcc gcgacgacac cgctccgctg atcgccgacg
     2401 gtgagatcgt ggccttcgag ggggatctca cctcgttctc gcgcctccag gaacggatgc
     2461 agaacgcgag acccagcgcg gcgcagatag cggccgtgcc ggtggtcttc tacctgttcg
     2521 acctgatctg gttcgacggc tacgacctct ccgcgttgcc gctggtcgcg cggaagtcgg
     2581 tgctgcacgg cgcgctcgcc ttcgctgacc cgatccgctt ctccgagcac ctggacgagg
     2641 agggcgaggt cgccttccgc gccgcgtgcg agaaggggtg ggagggcctc atcgccaagc
     2701 gtgcctcggc cccctacaca cacgggcgct cgaaggagtg gctcaagttc aagtgcgtga
     2761 acgaacagga gttcgtggtg ctggggtgga ccgatccgca cgggtcgcgc tccgggctcg
     2821 gagccctgct ggtcggctac cacgaagacg gcgccctgcg gttcggcggc aaggtgggta
     2881 ccggcttcaa cgagcgtgag ctcaccaggc tcacggcgat cctccgcccc ctcgagcaga
     2941 cggatccacc ggtcggcgac gtgaaggacc tgtcgacgaa gggggtgcac tgggtacgac
     3001 ccgagctcgt cgcacaggtc ggcttcagcg aatggacgcc ggacggcaag ctccgacatc
     3061 cccgctatct cggcctgcgc gacgacaagc gtcccaagca agtcgtccgc gagcgagcgt
     3121 aggagaggaa ggccgcgtca cccgccgtgt tcgtgcgtct gctcgttgcc cgcgtggagc
     3181 tcgtgggccg cctgcaccgc gtcctcccgc cggatgagtc caacgagcag gccgtcggag
     3241 gtcgtgatcg gcgagctcgg aaggtcgtgc gacaccatga acgcggccat ctccgcgata
     3301 gggacgtacg ggcgaaaggt gctcggcccc ggacgcatca cccgctcgat cgactcgtca
     3361 ccgtcccgct cgagctgcgc atgccggagc aggccgaaca ccacccgctc gtcgttcacg
     3421 accacgcacg cgtcccaccc cgcctcgcga acgcgttggc gaaccgggcc gagcagctcg
     3481 tccagccggc acgtcgggac gtctcggcga gccacggttc ccgcccgcgg gagttgggcg
     3541 ttcgtcccct cggtgggaag ccccgcggca agccagtcga gcttcccatc cacgtagtcg
     3601 tggacctcgg cgaagccgag cgcttcgagc cgccacgcgg ctcgtgggct gaggtctcag
     3661 gccgagtccc agcagtacac gacgatctgc cggtcgccat cgagccgctg ccgggcctcg
     3721 gccacgatct tccgaagggg cagatggatc gcgcccggga ggtggtcctc ctcgaactcg
     3781 tcccggggca ggacctcgac gatctgcgcg ccgcgctcca tgagcgcctg tacctcgcgt
     3841 cgttcgtgga tctcgtgggg catgcgacct cctcgcgtcg atcgggtggg cgaccaccgc
     3901 cgcggtgctg ttaccccgat ggctccgatg ggagacctcc gcccgaggac aggacgacgg
     3961 ccgctcgaac gcggtcgcga aggcccacgg gggcgggttc gcgggcgtcc tcggcgatcc
     4021 gacggccgaa gcgaccgagc agcacctcgc gccagcagtc gtcgcagtcg agcaggtgtc
     4081 gttcgaacca tcgacgcgcc cggacggaga gctcacccga gacgtactcg gccgagtcac
     4141 gctccggatc gtgacgccgg ttccgctgcg gtcgaatcat cgcgtccccc cacgtacctc
     4201 gtcccacagg agcgcggcga gtccccgccg gccccgatgc aaccgcgaga gcaccgtgcc
     4261 tcgcgggacc cccatcgcct tcgccgcttc agccgtcttc agccccacga ggtccacgag
     4321 cgtgatcgcg acgcgttgct cctcgggcag ccgccgcatc gcctcgtaca gggcctcccg
     4381 gtccaaggcc gcctcggcgg ctcgctccgg atccgaggtg gccggtgccc ggaagtctcg
     4441 gtcctcgatc gagacctcga tcggacgacg cccgcgggac cggtggcggg agcgtccgag
     4501 gttcaggcag atcgtggcga gccagggctc gacccgacgc ggacggcgcc ggtccctcca
     4561 ggcccggtac gccgcgaggt acgtctcctg cacgaggtcc tcggcgtcct ccggccgcac
     4621 ggcgaaccgc cgcgcgaggt tgtagacgag atccatcgcc cccatggtcg cgcggaggaa
     4681 ttcctcgtgg tcgtcgcgca cggtgaaggt ctacttgtcg ggcttcgatc ccgcttcctc
     4741 gccaccgtcg ccgtgggcgc tcgcgacgtg acggtcgagc tcctcctgcg tctcgaacga
     4801 cgcaccgcac ttcgcgcaga tgtgtccccc gttctgctcc tgtccctcca tggttcctcc
     4861 ctctcgtcgg tcaggccgcg gcctgccgca cggatcgccg tgctctcgcc tcggacccct
     4921 tgaacagtgg ccacaggccc aggacgaagc cgtagagcgc gtgctcgacg gtgaacgtgc
     4981 cccatcccgc cagccgaggc atctccgaga tgggatctcc gcccccgaac acgtcggcga
     5041 tcaccgggag cgcgacgaag gacatcacga gcatcacggc cagcccgtag acgatgccgg
     5101 cgagcagccc cgcccagccg ttcagcttgc cggtggacac gatgaggccg aagatcgcgc
     5161 cccagaacgc gccggtgagc aggtggagcg ccacgcccac cgccgccggc cccgccgaga
     5221 acgtgaaggc gtctccgttc gctgccgcct ccatcgagcg catcatcgtc gccgggtcga
     5281 tgagcgagga ggcgatgtgg tagagcggcg cgaagaagcc ggtgccctgg tacgtcgcgg
     5341 ccgcgaccat cgcgaacatc gccatgacga tcgcggcgac gacacccacg agcgcgcccg
     5401 tcaggacgtg actcaaggac accgaggcgg atctcgagat cgatcctgcc gtggccatgt
     5461 gacctccttt ccacccttgc atggatggac gccggcggag cggattgatt ccacggg
//
LOCUS       DATBRK010000282         6464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2210786, whole genome shotgun sequence.
ACCESSION   DATBRK010000282 DATBRK010000000
VERSION     DATBRK010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210786"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VIH70_08500"
     CDS             <1..159
                     /locus_tag="VIH70_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEY8116439.1"
                     /translation="NYILKVRARTTSDLEDLIQRLREKAEVNTTTTVVLSVPFEGRPL
                     RPSPKPPT"
     gene            complement(156..770)
                     /locus_tag="VIH70_08505"
     CDS             complement(156..770)
                     /locus_tag="VIH70_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116440.1"
                     /translation="MPRDPFVELESAREAVLAEAERFVQELEPVLGPSLHAGFERLRQ
                     AQAGHAAGLDGPTLAALEDAVDRAVEVGVAETVERLRSPQIWLSPLTAPELPPRTDPA
                     RPLGLPEWVARLGGSAREGSALGRLDEMTNRIWVAISAAAKPLDPVLEEFGFRPERHR
                     LGGGRFGVSPRNLPQLDPSGAIGQRWKRYRAAFERLTALAAFDA"
     gene            880..2688
                     /locus_tag="VIH70_08510"
     CDS             880..2688
                     /locus_tag="VIH70_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8116441.1"
                     /translation="MDQYRRYGRWLKRAAWVLAIYLFAAWLFMGLSPSQSISNPSLVV
                     TQLRPFMLQALILVFFIILQFGALFWFLSRGNDYVIYPNEYDTSFDDVRGQPAAVEAT
                     QEVLRVFEGFKDFKQMGGYPPHGILFEGPPGTGKTLMAKAIAGASGVPFLFATGSGFA
                     NMFMGIGNLKVRKLFKKGRVMSDKYGGSVIFIDEIDAVGSRGAVSTERSADRATDRIF
                     MGAGMGGGSGMGVINELLVQMDGFTVPRGLWRHVRRIAFRAKPKVPFYNILVIGATNR
                     ASTLDPALVRPGRFDRKIHVGLPDGEGREDILRYYLAKVRHEPIDQTKLAAMTVGYSP
                     AALKNIVNEALLFALQDGRDALRWDDLWQAKLTEEIGLKQPVKYSPREKEMVAVHEAA
                     HAVAAYALRRGEQVVQVITIQKRESALGLVSRQDVAESFLRTQRQILADIQISLAGWV
                     AEDIWYDQVTSGPSSDLATATYWAARYVGEFGMGKSLISASLDADQIEDSIGATLGDR
                     ERRKEIDALLSDCRERVRNLLLKKRHVVEGVRNALLEREELVGDEIEVLMAELGEREP
                     IEVSAAADGDGTGGFPPEGNGRPGDGNGHRPAPPHP"
     gene            2730..4121
                     /gene="cysS"
                     /locus_tag="VIH70_08515"
     CDS             2730..4121
                     /gene="cysS"
                     /locus_tag="VIH70_08515"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY8116442.1"
                     /translation="MTVRLYDSLTRELHDVSPVDGETVKIYSCGPTVYRYIHIGNMRS
                     FMLGDLIRRALRFDGFRVRWIMNITDVGHMTDEVSDTGRDKMELAEADEGLSAADIAR
                     KYTDAFLEDSDLVGIERADLYPRATDHIDDMIKIIQTLIEKGHAYEVDGTVYYDVSSF
                     PGYGKLSGNTLDQLRAGHRQEVAVDPAKRHPEDFALWKKAGPNRALKWPSPWGDGYPG
                     WHIECSAMSMKHLGERFDIHTGGNDNKFPHHEDEIAQSEAAVGHPVVSTWVHGGFLRM
                     GKVKMAKSAGNILRVTDLAAQGVDPLAYRLLCFGTRYRSEMNFDWDALEGQNRRLTEL
                     RRRVADWGPASSDRSDAARSLDARFREAVADDLDLPRALVVLNETVSAPVPDGEKVDL
                     MTSWDQLLGLDLGRLAREAFEPSEEALALIAKRDEARAAKDFARSDELRDRLQAMGLE
                     VMDTAEGTKVRPR"
     gene            complement(4147..5247)
                     /locus_tag="VIH70_08520"
     CDS             complement(4147..5247)
                     /locus_tag="VIH70_08520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006136512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY8116443.1"
                     /translation="MSADRYADRRLRSLEAAVAAGLGGILASPGPDLTYLTAYTPPPL
                     DRLTLLVLSTGADPTLMMPTLERPLAESAAGAPGLTMLDWRDGSDDPYEIVTAMLGSG
                     RYAVTDRTWASHLLALQRAAPDRSFVAASEAVPLLRAVKDADEIQRLRAVAKGADAAF
                     ADVIELPFAGRAETEVAAELDRLLREHGHQRVDFTIVGSGPNAASGHHEPGERRIEPG
                     DAVVMDFGGVRDDYCSDITRTVFVGEPSREEREVYAIVRAAQQAAFEAVRPGVAAQDV
                     DRAARSVITEAGYGERFVHRTGHGIGLEIHEPPYIVEGNRTPLEAGMTFSDEPGIYLE
                     GRFGVRIEDQVAVTAQGAERLNEASRELTVVA"
     gene            complement(5244..6173)
                     /locus_tag="VIH70_08525"
     CDS             complement(5244..6173)
                     /locus_tag="VIH70_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY8116444.1"
                     /translation="MPSSYGELSVIVNPHAGRRRVGQEIPELERTLRSRGLPYRLFRT
                     EGPGDATRLATEALEAGGRFVVAVGGDGTVHEVVNGMLDGEGKPLVPDAVLGVVAAGS
                     GCDFVRTFGLPGDATRACSHLAGENTYPLDVGRVTTTTVEGGRATRYFVNVAEAGLGA
                     AVAARAERMSPRLGQSKYFIGFWLELPRFKLANVRVRADRRTYEGPAFLVVVGNGQYY
                     GGGMKISPRSYPGDAVLDILVFKGPKSDSFTMIPKIYRGEHIPHDHVEEFRVKRELVI
                     EADRPLPIESDGEVLGTTPATFEIIAQPIQMKL"
     gene            complement(6224..>6464)
                     /locus_tag="VIH70_08530"
     CDS             complement(6224..>6464)
                     /locus_tag="VIH70_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116445.1"
                     /translation="LCRELEDGFAAPIHRWAQGESLEDVLRETDMSPGDFVRTCKQVL
                     DLLRQIEEVAPQGVADLVRRAREAVNRGVVAYTGV"
BASE COUNT         1001 a         2265 c         2256 g          942 t
ORIGIN      
        1 aactacatcc tcaaggtgcg cgcgcgaacc acctcggacc tcgaggacct gatccagcgg
       61 ctccgcgaga aggccgaggt caacacgacg accaccgtgg tgctctcggt gccgttcgag
      121 ggccggccgc tccgaccgag cccgaagccg ccgacctagg cgtcgaaggc cgcgagcgcg
      181 gtcagacgct cgaacgcggc tcgatagcgc ttccaccgct gcccgatcgc gccgctcgga
      241 tcgagctgcg gcaggttcct cggcgagacc ccgaacctcc cgccgcccag ccgatgccgc
      301 tcgggccgga acccgaactc ctccaggacc gggtcgaggg gcttggccgc cgccgagatc
      361 gcgacccaga tccggttcgt catctcgtcg agcctgccga gcgccgagcc ctcgcgggcc
      421 gagccgccca gccgggcgac ccactccggc agcccgagcg gccgggcggg gtcggtgcgc
      481 ggcgggagct cgggcgcggt gagcggcgag agccagatct ggggcgagcg caggcgctcg
      541 accgtctcgg ccacccccac ctcgaccgcc cgatccaccg cgtcctccag cgcggccagg
      601 gtcggcccgt cgagcccggc agcatgaccg gcctgcgcct gccgcaggcg ctcgaacccc
      661 gcatgcaggc tcgggcccag gacgggctcg agctcctgga cgaaccgctc ggcctcggcc
      721 aacacggcct ctctcgccga ctcgagctcg acgaacggat ccctcggcat cccgcgagct
      781 taggaggctt cgagccgcgg ggtgtccgca accatggtgc gcgcgcgtag actcgggcaa
      841 cgaccgggcc catgctcgag cgggaggagg tcgaccgcca tggatcagta ccgtcgttac
      901 ggccgttggt tgaagcgggc cgcgtgggtg ctcgcgatct acctgttcgc cgcctggctg
      961 ttcatgggtc tgtcgccctc gcaatcgatc agcaacccat ccctcgtcgt gacgcagctc
     1021 cggccgttca tgctgcaggc gctgatcctg gtcttcttca tcatcctgca gttcggcgcg
     1081 ctgttctggt tcctgtcgcg cggcaacgac tacgtgatct accccaacga gtacgacacg
     1141 tcgttcgacg acgtgcgcgg ccagccggcg gccgtcgagg cgacccagga ggtgttgcgg
     1201 gtcttcgagg gcttcaagga cttcaagcag atgggcgggt acccgcccca cggcatcctg
     1261 ttcgagggcc cgcccggcac cggcaagacg ctgatggcca aggcgatcgc gggagcctcc
     1321 ggcgtgccgt tcctgttcgc gacgggctcg ggcttcgcga acatgttcat gggcatcggg
     1381 aacctgaagg tgcgcaagct tttcaagaag ggccgagtca tgtcggacaa gtacggcggg
     1441 tccgtgatct tcatcgatga gatcgacgcc gtcggttcaa ggggcgccgt gtcgaccgag
     1501 cgatccgcgg atcgagcgac cgaccggatc ttcatgggcg ccggtatggg cggcggctca
     1561 ggcatggggg tcatcaacga gctgctcgtc cagatggatg gcttcacggt cccacggggc
     1621 ctgtggcggc atgtccggcg gatcgcgttc cgcgccaagc cgaaggtgcc cttctacaac
     1681 atcctcgtga tcggggcgac gaaccgcgcc tcgacgctcg atccggcgct cgtccgcccg
     1741 ggtcggttcg atcgcaagat ccacgtgggg ctcccggacg gcgagggtcg cgaggatatc
     1801 ctgcggtact acctggcaaa agttcgccac gagccgatcg atcagacgaa gctggccgcc
     1861 atgaccgtcg gctactcgcc tgcggcgctg aagaacatcg tgaatgaggc gcttctgttc
     1921 gctctccagg atggacgcga cgcgctgcga tgggacgacc tgtggcaggc caagctcacg
     1981 gaggagatcg gtttgaagca gccggtcaag tacagcccac gcgaaaagga gatggtcgcg
     2041 gttcacgagg cggctcacgc ggtggccgcg tacgcgctcc gccggggcga gcaggtcgtc
     2101 caggtcatca cgatccagaa acgcgagtcc gcgctcggcc tcgtgagtcg ccaggacgtc
     2161 gccgagtcgt ttctacgaac ccagaggcag atcctggccg atattcagat ctcgctggcg
     2221 gggtgggtcg ccgaggacat ctggtacgac caggtgacgt ccgggccctc cagcgacctc
     2281 gccacggcca cctactgggc ggcgcgctac gtgggggagt tcggcatggg caagagcctc
     2341 atatccgcct cgctggacgc cgaccagatc gaggactcga tcggcgcgac gctcggcgac
     2401 cgggagcgcc gcaaggagat cgacgcccta ctcagcgact gccgcgagcg ggtacgcaac
     2461 ctcctcctca agaagcgcca cgtcgtcgag ggagtacgga acgcgttgct cgagcgcgag
     2521 gagctggtcg gcgatgagat cgaggtcctg atggccgagc tcggcgagcg ggagccgatc
     2581 gaggtctcgg ccgccgctga cggagatggg accggtgggt tcccgcccga gggcaacggg
     2641 cggcccggcg acggtaacgg gcaccggccg gcgccgccgc atccttagac cccgccgaat
     2701 gccgtcgccg atcgcgacga tatgcttgcg tgaccgtgcg tctgtacgac tccctcacgc
     2761 gtgagcttca cgacgtctcc cccgtcgacg gggagacggt gaagatctac tcgtgcggcc
     2821 cgaccgtcta ccggtacatc cacatcggga acatgcggtc gttcatgctg ggtgacctga
     2881 tccgccgcgc gctgcgcttc gatgggttca gggtccgctg gatcatgaac atcaccgacg
     2941 tcggtcacat gacggacgag gtctcggaca ccggccgcga caagatggag ctcgccgagg
     3001 ccgacgaggg cctgtcggcg gccgacatcg cgcgaaagta caccgacgcg ttcctggagg
     3061 actccgacct ggtcgggatc gagcgagccg acctgtaccc gcgcgccacc gaccacatcg
     3121 acgacatgat caagatcatc cagacgctga tcgagaaggg gcacgcgtac gaggtcgacg
     3181 gcaccgtgta ctacgacgtc tcctcgttcc ccggttacgg aaagctctcg ggcaacacgc
     3241 tcgaccagct gcgcgcgggc catcgccagg aggtcgcggt cgatccggcc aagcgccacc
     3301 ctgaggactt cgcgctttgg aagaaggccg gcccgaaccg ggcgctcaag tggccgagcc
     3361 cgtggggcga cggctacccg ggatggcaca tcgagtgctc ggcgatgtcg atgaagcacc
     3421 tgggcgagcg cttcgacatc cacaccggcg gcaacgacaa caagttccct caccacgagg
     3481 acgagatcgc gcagtcggag gccgccgtcg ggcacccggt ggtctcgacg tgggtgcacg
     3541 gcgggttcct gcggatgggc aaggtgaaga tggccaagtc cgcggggaac atcctgcggg
     3601 tcaccgacct ggcggcccag ggcgtcgacc cgctcgccta ccggctgctc tgcttcggca
     3661 cccggtaccg cagcgagatg aacttcgact gggacgcgct cgaggggcag aaccgccggc
     3721 tcacagagct ccgccggagg gtggccgact ggggccccgc gtcgtcggac cggtccgacg
     3781 cggcccgatc cctcgacgcg cgtttccggg aggccgtggc cgacgacctc gacctgccgc
     3841 gcgcgctcgt ggtgctgaac gagaccgtgt ccgcgccggt ccccgacggc gagaaggtcg
     3901 acctgatgac gtcatgggac cagctgctcg gcctggacct cggccgcctc gcgcgcgagg
     3961 ccttcgagcc gtccgaggag gccctggcgc tgatcgccaa gcgggacgag gctcgggccg
     4021 ccaaggactt cgcccgcagc gacgagctcc gtgaccggct gcaggcgatg ggcctggagg
     4081 tcatggacac cgcggagggg acgaaggtcc ggccgcgctg agagaggccc agcggttccg
     4141 tcgccgtcag gcgacgaccg tgagctcccg gctggcctcg ttgagccgtt ccgcgccctg
     4201 cgcggtcacc gcgacctggt cctcgatccg gacgccgaac cgcccctcca ggtagatccc
     4261 cggctcgtcc gagaaggtca tgcccgcttc gagcggcgtc cggttcccct cgacgatgta
     4321 cgggggctcg tggatctcga gcccgatccc gtggccggtc cggtggacga agcgctcgcc
     4381 gtagcccgct tcggtgatca ccgaccgagc cgcccggtcg acgtcctgcg ccgcgacgcc
     4441 cgggcggacc gcctcgaacg ccgcctgctg cgccgcccgg acgatcgcgt agacctcgcg
     4501 ctcctcccgc gagggttcgc ccacgaagac ggtccgggtg atgtcggagc agtagtcgtc
     4561 gcggacgccc ccgaagtcca tcacgacggc gtcgccgggc tcgatccgtc gctcccccgg
     4621 ctcgtggtgt ccggaggcgg cgttcggacc cgaacccacg atcgtgaagt cgacgcgctg
     4681 atggccgtgc tcgcgcagca ggcggtcgag ctcggcggcg acctcggtct cggcgcggcc
     4741 ggcgaacggc agctcgatca cgtcggcgaa cgcggcgtcg gcccccttcg cgacggcgcg
     4801 gagccgttgg atctcgtcgg cgtccttcac cgcccggagc agggggacgg cctcgctcgc
     4861 ggccacgaag gagcggtcgg gcgccgcccg ctggagcgcg agcaggtgcg acgcccaggt
     4921 ccggtcggtc accgcgtagc gtcccgagcc gagcatggcg gtaacgatct cgtacggatc
     4981 gtcgctgccg tcgcgccagt cgagcatcgt gaggcccggt gccccggccg ccgactccgc
     5041 gagcgggcgt tcgagcgtcg gcatcatcaa cgtcggatcg gccccggtcg agagcacgag
     5101 cagcgtcaac cggtcgagcg gcggcggcgt gtacgccgtg aggtaggtca ggtctggccc
     5161 cggggaggcg aggatcccgc cgaggcccgc ggccaccgcg gcctcgaggc tccggagacg
     5221 cctgtccgcg tagcggtccg cgctcacagc ttcatctgga tcggctgggc gatgatctcg
     5281 aaggtggccg gggtcgttcc cagcacctcg ccgtcggact cgatcgggag cggacggtcg
     5341 gcctcgatca cgagctcgcg cttgacgcgg aactcctcga cgtggtcgtg ggggatgtgc
     5401 tcgccgcggt agatcttcgg gatcatcgtg aacgagtcgg acttcggacc cttgaacacc
     5461 aggatgtcga gcaccgcgtc gcccggatac gaacgaggcg agatcttcat gccgccaccg
     5521 taatactggc cgttgccgac caccacgagg aaggccggcc cctcgtaggt cctgcggtcg
     5581 gcccggacgc gcacgttggc gagcttgaac cggggcagct cgagccagaa gccgatgaag
     5641 tacttggact ggccgagccg cggtgacatg cgctctgcgc gagcggcgac cgccgcgccg
     5701 agacccgcct cggcgacgtt gacgaagtag cgcgtggctc gtccaccctc gacggtcgtg
     5761 gtggtcaccc tgccgacgtc gagcgggtac gtgttctccc cggccaggtg ggagcaggcc
     5821 cgcgtcgcgt cgccggggag gccgaacgtc cgcacgaagt cgcacccgct ccccgcggcc
     5881 acgaccccga gcaccgcatc gggcacgagc ggcttgccct cgccgtccag catgccgttc
     5941 acgacctcgt ggaccgtgcc gtccccgccc accgcgacca cgaagcgccc gccggcctcg
     6001 agggcttcgg tggccaggcg ggtcgcgtcg cccggaccct ccgtccggaa caggcgatag
     6061 ggcaggcctc gcgagcggag cgtccgttcg agctcgggga tctcttgccc gacgcggcga
     6121 cggcccgcgt gcgggttcac gatcacgctg agctcgccgt aggaactcgg catcgacgcg
     6181 catcctactt cggcgcaggg cgggcggttg cgacgcggtt cgatcagacg ccggtgtagg
     6241 cgacgacccc tcggttgacc gcctcgcggg cgcgacgaac caggtccgcg accccctggg
     6301 gggccacctc ctcgatctga cgcagcaggt cgagcacctg cttgcaggtg cgcacgaagt
     6361 cccccggcga catgtccgtc tcgcgcagga cgtcctcgag cgactccccc tgcgcccacc
     6421 gatggatcgg cgccgcgaac ccgtcctcga gctcgcggca gagc
//
LOCUS       DATBRK010000283         3205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1921181, whole genome shotgun sequence.
ACCESSION   DATBRK010000283 DATBRK010000000
VERSION     DATBRK010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921181"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VIH70_08535"
     CDS             <1..841
                     /locus_tag="VIH70_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY8116446.1"
                     /translation="LPTIRGERQECYAITEEVAGSDVDAIQATARRDGDEYVLNGRKW
                     HVTSFGSADYVFFQAKIEGGPNAGSHAMFFVDKDTPGVRPVREPAYTHTLIDTHVELA
                     LEDVRVPAANLIGSEGDGLRFVYSWFRYERLMIAARCCGAAERLIDEATAFAKDRVQF
                     GRSIIENQAIAHMLADSVTELWAARLMTYELAKNIDRGVDLKVQHAQCSMAKLYASEM
                     ANRVADRAVQIFGGRGYMRENVAERFWREVRVDRIWEGTSEIQRNIVADQLAKRGVRM
                     LSG"
     gene            841..1284
                     /locus_tag="VIH70_08540"
     CDS             841..1284
                     /locus_tag="VIH70_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8116447.1"
                     /translation="MDLHELLDKEASSWEALMAAVGRVPQDRRNEPGVVPGWSVADLV
                     WHCGYWADDAARRIGSIAAGKPEPDDPEAVWQKTNDEVAEESKAMSWDEVVTRSEAAR
                     ERIRTVLSSLSEIGPAAESEFSDETFEHYNEHTAEIERFAAGSGL"
     gene            complement(1347..2000)
                     /locus_tag="VIH70_08545"
     CDS             complement(1347..2000)
                     /locus_tag="VIH70_08545"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8116448.1"
                     /translation="MSTGTVKQGKDRHQEILDAAARVITDRGLAETRISDIAEEAGVS
                     PGLILYYFDSKDRLLAEALTFANDQFYIRTSREIRRIPSARDQLRRLVDLSVPGYLPE
                     YGRLDEWALWIEVWVRALRDAEMAKDREVLDQRWRSQLADLIREGQASGEFSSSEDAG
                     ELALRLATLIDGLAIQVIMNDSEVSAERMHRACMEVSAQQLGFELEPSKASASLPST"
     gene            2201..>3205
                     /locus_tag="VIH70_08550"
     CDS             2201..>3205
                     /locus_tag="VIH70_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8116449.1"
                     /translation="MANVYDAIVIGGGHNGLTAGAYFARAGARTVVLEARSKTGGAAD
                     TSAPFADHPEINVTTYSYVMSLMPPTIIKELNLKRHGYDVTPFGPYYQAYPDGRSIKV
                     YADDAAKSHDSIGQFSKKDAEVLPKWEAWLQEVADALGPLLLQKPPKIGSMAFGDLIE
                     SARAAWKMRKLGVRGVGDVTRLFTMSITDLLDDWFESDEIKGMLAVNGIIGTWAGPDE
                     PGTAYVMLHHSIGDVGDGHLGSWGFQQGGMGAVSDSIRRSAESYGCEIRTDARVRKMV
                     TAGGRFAGVVLDNGEELRAPLGVTCIHPKIAFLDLIGREELPADFVWDIERWRTRSGV
                     V"
BASE COUNT          526 a         1050 c         1138 g          491 t
ORIGIN      
        1 cctgccgacg atccgcggcg agcgccagga gtgctacgcg atcacggagg aggtcgccgg
       61 ctccgacgtc gacgcgatcc aggccacggc gcggcgggac ggcgacgagt acgtgctgaa
      121 cggcaggaag tggcacgtca cctcgttcgg ctcggccgac tacgtcttct tccaggccaa
      181 gatcgagggc ggcccgaacg cgggctcgca cgcgatgttc ttcgtcgaca aggacacgcc
      241 gggggttcgg cctgtccgcg agcccgcgta cacccacacg ctgatcgaca cccacgtcga
      301 gcttgcgttg gaggacgtgc gtgtcccggc cgccaacctg atcggatccg agggggacgg
      361 gctgaggttc gtgtactcct ggttccgcta cgagcgcctg atgatcgccg cccggtgctg
      421 tggcgccgcg gagcgcctga tcgacgaggc gacggcgttc gcgaaggatc gtgtgcagtt
      481 cggcagatcg atcatcgaga accaggcgat cgcccacatg ctggccgatt cggtgaccga
      541 gctgtgggcg gcgcgcctga tgacgtatga gctcgccaag aacatcgatc gcggcgtcga
      601 cctcaaggtc cagcacgccc agtgctcgat ggccaagctg tatgcgagcg agatggccaa
      661 tcgggtcgcc gaccgggccg tccagatctt cggcggccgc ggttacatgc gcgagaacgt
      721 cgccgagcgc ttctggcgcg aggtccgcgt cgaccggatc tgggagggca cgtccgagat
      781 ccagcggaac atcgtggcgg accagctcgc caagcgcggc gtgcgtatgc tgagcggctg
      841 atggaccttc acgagctcct cgacaaagag gcatcgtcct gggaggcgct gatggccgcg
      901 gtgggccgtg tgccgcagga tcgcaggaac gagcccgggg tggtgcccgg atggtccgtc
      961 gccgacctcg tgtggcactg cgggtattgg gccgacgacg cggcccgtcg gatcgggtcc
     1021 atcgcggcgg gcaagcccga gcccgacgac ccagaggccg tctggcagaa gacgaatgac
     1081 gaggtggccg aagagtccaa ggcgatgtcc tgggacgagg tcgtcactcg ttccgaggcg
     1141 gcgagggaga ggatcaggac ggtcctgtcg tcgctgtcgg agatcgggcc cgccgccgag
     1201 tccgagttct cggacgagac gttcgagcac tacaacgagc acacggccga gatcgagcgg
     1261 ttcgccgcgg gctccggcct ctgagctcgg cgcccggcgg ccgccagctc gcctacaggg
     1321 tcctcactcg atcggccctc gcgcggtcac gtcgagggaa gtgacgcgga ggccttcgac
     1381 ggctcgagct cgaacccaag ctgctgcgcc gagacctcca tgcaggcgcg gtgcatacgc
     1441 tcggccgaga cctccgagtc gttcatgatc acctggatcg cgagcccgtc gatcagcgtc
     1501 gcgagccgga gcgcgagctc gcccgcgtcc tcgctcgagg agaactcgcc gctggcctgg
     1561 ccctcgcgga tgaggtcggc gagctgcgag cgccaccgct ggtcgagcac ctcccggtcc
     1621 ttggccatct cggcgtcgcg cagcgcccgc acccacacct cgatccacag cgcccactcg
     1681 tccaggcgtc cgtactcggg caggtagccg ggcaccgaga ggtcgaccag ccggcgaagc
     1741 tggtccctcg ccgaggggat cctgcggatc tcgcgggagg tgcgaatgta gaactggtcg
     1801 ttcgcgaacg tgagcgcctc ggcgagcagg cgatccttcg agtcgaagta gtacaggatc
     1861 agcccgggcg agacgccggc ctcctcggcg atgtcggaga tgcgcgtctc cgcgagtcct
     1921 cggtcggtga tgacgcgcgc cgcggcgtcc aggatctcct ggtgccgatc cttgccctgc
     1981 ttgacggtgc cggtgctcac ggttccgctc ctgttgaacg cgggccaatc ctggacggtt
     2041 tctcgcgggt gtttgaaccg aggttcagca actctacgcc cctgacctgg cctgtcaacc
     2101 gacacgagag tgatgctcgc ttgaccggcc attcaaacgc atgggaggat ggacgccccg
     2161 gccggagcgc ggccgcaccg gataggagac cggaacgccc atggcgaacg tctacgacgc
     2221 gatcgtgatc ggcggtgggc acaacgggct gaccgcgggg gcgtacttcg cacgcgcggg
     2281 ggcaaggacc gtggtgctcg aggcacggag caagaccggc ggcgccgccg ataccagcgc
     2341 gccgttcgcc gatcatcccg agatcaacgt gacgacctac tcgtacgtga tgtcgctgat
     2401 gccgcccacc atcatcaagg agctgaacct caagcgtcac gggtacgacg tcaccccgtt
     2461 cggcccgtac taccaggcct accccgacgg acgatcgatc aaggtctatg cggacgacgc
     2521 cgcgaagagt cacgactcga tcgggcagtt ctccaagaag gacgccgagg tcctgccgaa
     2581 gtgggaggcg tggctccagg aggtcgccga cgccctcggt cccctgctcc tgcagaagcc
     2641 gccgaagatc ggctcgatgg cgttcggcga cctgatcgag agcgcacgag ccgcctggaa
     2701 gatgcgcaag ctcggcgtcc gcggggtcgg cgacgtcacg cgtctgttca cgatgagcat
     2761 cacggacctg ctcgacgact ggttcgagtc cgacgagatc aaggggatgc tcgcggtcaa
     2821 cgggatcatc gggacgtggg cggggcccga cgaacccggc acggcctacg tgatgctgca
     2881 ccactcgatc ggtgacgtcg gcgacgggca cctcggttcg tgggggttcc agcagggcgg
     2941 gatgggcgcg gtgtccgatt cgatccggcg ttcggccgag tcctacgggt gcgagatccg
     3001 caccgacgcg cgcgtccgga agatggtgac ggcgggcggc cggttcgccg gcgtcgtgct
     3061 cgacaacggc gaggagctgc gggcgccgct cggcgtgacc tgcatccacc cgaagatcgc
     3121 gttcctcgac ctgatcggtc gcgaggagct gcccgccgac ttcgtctggg acatcgagcg
     3181 gtggcgcacg cgttcgggcg tcgtg
//
LOCUS       DATBRK010000284         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2358197, whole genome shotgun sequence.
ACCESSION   DATBRK010000284 DATBRK010000000
VERSION     DATBRK010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358197"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /gene="dnaK"
                     /locus_tag="VIH70_08555"
     CDS             <1..1347
                     /gene="dnaK"
                     /locus_tag="VIH70_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY8116450.1"
                     /translation="VFDLGGGTFDVSVLELGEGVFEVKSTNGDNHLGGDNFDKAVVDW
                     MVAEFKREQGIDLSQDKMALQRLYEAGEKAKIELSSTMATQINLPFITATQEGPKHLD
                     LQLTRAKLNELTADLLDRTVGPTKQALADAGIEPSGIDHVVLVGGMTRMPAVVDKVRD
                     LIGKDPHKGVNPDEVVAVGAAIQAGVLRGEVKDVLLLDVTPLSLGIETKGGVFTPLIE
                     RNTTIPTKKSETFTTAEDNQPSVEVHVLQGESEMALYNKTLGKFQLVGIPPAPRGMPQ
                     IEVTFDIDADGIIHVSAKDVGTGNEQKIRIEGGSGLKQEEVERMIREAESHSDEAHRL
                     RELADARNAAESLAYQTERTLKDNRDKLDEADASTIEGRVMELRGVLESSDINEIRQK
                     TEALQEAAQKLAEAVYAQASAQQQASGPADGDGAAASDDEVIEDAEIVDEEGAKTS"
     gene            1347..>1684
                     /gene="grpE"
                     /locus_tag="VIH70_08560"
     CDS             1347..>1684
                     /gene="grpE"
                     /locus_tag="VIH70_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY8116451.1"
                     /translation="MADPAREQLDQSELRDQVPGRVPGPGPEVDERLALAEAKAQEHL
                     DDLKRLAAEFENYKKRTAREQASLSTRATEHLVKELLPIVDDLERALEAAEEHEEVKL
                     EEGVRLVHRQL"
BASE COUNT          336 a          535 c          592 g          221 t
ORIGIN      
        1 gtcttcgacc tcggcggcgg gacgttcgac gtctcggtac tggagctcgg cgagggcgtc
       61 ttcgaggtca agtcgacgaa cggggacaac cacctcggcg gcgacaactt cgacaaggcg
      121 gtcgtcgact ggatggtcgc cgagttcaag cgcgagcagg gcatcgacct ctcgcaggac
      181 aagatggcgc tccagcgcct ctacgaggca ggtgagaagg cgaagatcga gctgtcgtca
      241 acaatggcga cgcagatcaa cctgccgttc atcaccgcga cgcaggaggg gccgaagcat
      301 ctcgacctgc agctcacgcg cgcgaagctg aacgagctga ccgccgacct gctcgaccgc
      361 accgtcggtc cgaccaagca ggcgctggcg gacgcgggca tcgagccttc ggggatcgac
      421 cacgtcgtcc tcgtcggggg catgacccgc atgcctgcgg tcgtcgacaa ggtgcgcgac
      481 ctgatcggca aggacccgca caagggtgtc aacccggacg aggtcgtcgc cgtcggcgcc
      541 gcgattcagg ccggcgtgct ccgcggtgag gtcaaggacg tgctcctgct cgacgtgacg
      601 ccgctgtcgc tagggatcga gaccaagggc ggcgttttca cgcccctgat cgagcgcaac
      661 acgaccatcc cgacgaagaa gtcggagacg ttcacgaccg ctgaggacaa tcagccctcg
      721 gtcgaggtgc acgtcctcca gggtgagtcg gagatggccc tctacaacaa gacgctcggc
      781 aagttccagc tcgtcggcat cccgccggcg ccgcggggaa tgccgcagat cgaggtcact
      841 ttcgacatcg acgccgacgg gatcatccac gtgtcggcca aggatgtcgg cacaggcaac
      901 gagcagaaga tccggatcga gggcggctcc ggtctgaagc aggaggaggt cgagcggatg
      961 atccgcgagg ccgagtccca ctcggacgag gcgcacaggc tccgcgagct cgcagacgca
     1021 cgcaacgcgg cggagagcct cgcctaccag accgagcgca cgctcaagga caaccgcgac
     1081 aagctcgacg aggccgatgc ctcgacgatc gagggtcgcg tgatggagct ccgcggcgtg
     1141 ctcgagagct cggacatcaa cgagatccgt cagaagacgg aggcgctgca ggaagcggcg
     1201 cagaagctcg ccgaggcggt ctacgctcag gcgagcgctc agcagcaggc ttctggcccc
     1261 gctgacggcg acggcgccgc ggcctcggat gacgaggtga tcgaggacgc cgagatcgtc
     1321 gatgaggaag gggcgaagac gtcctaatgg cggatcctgc acgggaacaa ctcgaccagt
     1381 ccgaactgcg ggaccaggtc ccgggccgtg tcccgggacc tggtcccgag gtcgatgagc
     1441 ggctcgccct cgcggaggcg aaggcacagg agcatctcga cgacctgaag cgcctcgcgg
     1501 ccgagttcga gaactacaag aagcgcactg cgcgcgagca ggcgagcctg tccacgcggg
     1561 ccaccgagca cctcgtcaag gagctgctgc cgatcgtgga cgacctcgag cgtgcgctcg
     1621 aggcggcgga ggagcacgag gaggtcaagc tcgaggaggg cgtgaggctc gtgcaccgtc
     1681 agct
//
LOCUS       DATBRK010000285         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1346471, whole genome shotgun sequence.
ACCESSION   DATBRK010000285 DATBRK010000000
VERSION     DATBRK010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346471"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VIH70_08565"
     CDS             complement(<1..372)
                     /locus_tag="VIH70_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY8116452.1"
                     /translation="MTYRSGPLVPTVVEESARGERAFDIYSRLLRERIVFLAEPIDDA
                     VATTVVAQLLFLDSVEPGKDIALYVNSPGGASTALFAIYDTMRFLRSDVATYCLGQAA
                     SAAAVILVAGTRGKRFALPNSR"
     gene            484..846
                     /locus_tag="VIH70_08570"
     CDS             484..846
                     /locus_tag="VIH70_08570"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8116453.1"
                     /translation="MRDERVTRSVVLPAGRDEVWAMLTTPEDLSAWLGEVVELEPEPG
                     GAIVLSEEGSTRRGMVEAAEPGRILSFRWRRLARAGVSLEVGGATRVTFVLEADPEGT
                     RLTVTEEPVLLATAGLGA"
     gene            843..1172
                     /locus_tag="VIH70_08575"
     CDS             843..1172
                     /locus_tag="VIH70_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8116454.1"
                     /translation="MSGDRSVNEVFSALSDPTRRAVIRSLSEDGPSTVTELAGRLPVT
                     RQAVTKHLEALEEAGLVASDARGRGARFRLTPRPMTEAMRWMADVGGEWDERLEALRA
                     HLARRRP"
     gene            1231..1704
                     /gene="nrdR"
                     /locus_tag="VIH70_08580"
     CDS             1231..1704
                     /gene="nrdR"
                     /locus_tag="VIH70_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY8116455.1"
                     /translation="MRCPWCGHDGDKVVDSRPAERGDAIRRRRQCLSCGRRFTTYERV
                     EQLALVVVKRDGTSEPWDREKMVSGIRKALVNRPITDEQFAALVSRIEGRLRRKGPEI
                     TSQRIGLEVLANLQKLDQVAYVRFASVYKDFQEIGDFEREFGLMLQKKESKARSR"
BASE COUNT          300 a          607 c          680 g          287 t
ORIGIN      
        1 ccgggagttc ggcagcgcga accgcttgcc gcgggtgccc gccaccagga tgaccgccgc
       61 ggccgaggcc gcctgaccca ggcagtaggt cgcgacgtcg gacctcagga accgcatggt
      121 gtcgtagatc gcgaacagcg ccgtcgacgc gccgccgggg ctgttcacgt acagcgcgat
      181 gtccttgccc ggctccaccg agtcgaggaa caggagctgc gcgaccacgg tggtcgcgac
      241 cgcatcgtcg atgggctcgg cgaggaacac gatccgctcg cgcaggagcc gggagtagat
      301 gtcgaaggct cgctcgcccc gcgcgctctc ttccacgacg gtggggacca gcggtccgga
      361 tcggtaggtc atcggatcct cctcgttcga tcgacgaaac gactcagcaa ccagcgggtt
      421 gccgagtgga tggtagccgc gttccgacgt gtcggcaacc cttcggttgc gtacgatgtc
      481 ggggtgcgcg acgaacgggt cacgaggtcg gtggtactgc cggcgggccg cgacgaggtg
      541 tgggcgatgc tgacgacccc ggaggatctg tcggcctggc tgggcgaggt ggtggagctc
      601 gagccggagc ccggcggggc gatcgtcctc agcgaggagg gatccacgcg gcgagggatg
      661 gtcgaggccg ccgagccggg caggatcctg tcgttccgct ggcggcggct ggcgcgggcg
      721 ggcgtctcgc tcgaggtcgg gggagccacg cgcgtgacgt tcgtccttga ggccgacccc
      781 gagggcacac gcctcacggt caccgaggag ccggtactcc tagcgacggc ggggctcggg
      841 gcatgagcgg cgatcgcagc gtgaacgagg tgttctcggc gctctcggat ccgacccgcc
      901 gagcagtgat ccgcagcctg tccgaggacg gaccgagcac cgtgaccgag ctcgccggcc
      961 gcctccccgt gacgcggcag gccgtgacca agcatctcga ggcgctcgag gaggccggcc
     1021 tcgtcgcatc cgacgcccga ggtcgcgggg cccggttccg gctcacgccg cgtccgatga
     1081 ccgaggccat gcggtggatg gccgacgtgg gtggcgagtg ggacgagcgt ctggaggcgc
     1141 tccgagcgca cctcgcacgc cgtcgtcctt gacgcacgct acatctcgcg gctaccgtgc
     1201 ctggatccac tagatgttgg ggtgatcgtg atgcgctgtc cgtggtgcgg acacgacgga
     1261 gacaaggtgg tcgactcccg ccccgccgaa cgcggcgacg cgatccggcg ccggcgccag
     1321 tgcctgtcct gcggacgccg gttcaccacc tacgagcggg tggagcagct cgctctggtg
     1381 gtcgtcaaac gagacgggac gagcgagccc tgggatcggg agaagatggt ctcgggcatc
     1441 cggaaggccc tggtcaaccg gcccatcacc gacgagcagt tcgccgcgct cgtctcgagg
     1501 atcgaggggc gtctgcgccg caagggtccc gagatcacct cacagcggat cgggctggag
     1561 gtgctggcca acctccagaa gctcgatcag gtggcctacg tccggttcgc gagcgtctac
     1621 aaggacttcc aggagatcgg cgacttcgag cgcgaattcg gcctcatgct ccagaagaag
     1681 gagtccaagg cccgctcgcg ctgaccgcta tatcttgtgg atgtcattcg tctcgaccac
     1741 aagatatcct tgatcgaacg cttgactgcg tcgaccggcg ggtgcagaat cacacgcctg
     1801 tagttctcca cagttccttc cccacccgtg gaaagactgg ccgggaaccg atcgacgagg
     1861 aggtgccggt ggcc
//
LOCUS       DATBRK010000286         4089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_48999, whole genome shotgun sequence.
ACCESSION   DATBRK010000286 DATBRK010000000
VERSION     DATBRK010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48999"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VIH70_08585"
     CDS             complement(<1..782)
                     /locus_tag="VIH70_08585"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY8116456.1"
                     /translation="MTWENSAITIATLLPLAGALVIALVPRQYEKGVRALGIVFTGAA
                     LVFAIAITFTFDYGASGLQFQLDTSWIPAIDARYHVGIDGISLPLYVLTFVLSFLCAI
                     YTWRVVPKPGKTKAFIALMLLLETGMAGTFIAFDLILFFVFWELVLVPMYFLIGIWGS
                     SNREYAAIKFFLYTLFGSVFMLLGFLGMFFSSDVGGGVHTFDIIALQDYAASGGFSHG
                     FQLIVFAAVGLGFAVKVPMWPFHTWLPDAHTEAPTIGSVLLAG"
     gene            complement(856..2844)
                     /gene="nuoL"
                     /locus_tag="VIH70_08590"
     CDS             complement(856..2844)
                     /gene="nuoL"
                     /locus_tag="VIH70_08590"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY8116457.1"
                     /translation="MVAAESAESIVKAIQDPSWFLRNSWLIALLPAAAAAFTLFIGKR
                     TPGKGSVYGITALAAALVMSLGALLTFVGGERGFYEASIPWFQIGQLHMEVGVYVDGL
                     TAVMLVVVTSISLCVHIYSLGYMKGDVRYTWFYVVLSLFTSAMLVVVLANNLFQLLVG
                     WEVMGVCSYLLIGHWWEDPVNSGSAIKAFITTRVGDVPFMFGIFVLIFITGFTTSNIP
                     KISEIVSSGGASVGLVSAAALLLFGGTIGKSAQFPLHVWLPDAMAGPTPVSALIHAAT
                     MVAAGVYLVGRLFIVFLDADPFVLQIVAIVAGITMLGAALMAFTQDDIKRVLAYSTIS
                     QLAYMIAGMSMGPAGRTGAFFHLFTHAFFKALLFLGSGAVIHAVHSNNMSEMGGLRRR
                     LPITFWTFLIGSLALSGVPPLAGFWSKDELLVVAEHEGHPVLFVVLLATALLTAFYMT
                     RAVLMTFYGEYRGHGEPHEAPRSMTGVLVALASVTVVAGLVGSPQLGAVFGKWVYFRE
                     LEEALFVPWVAVLSTLAALGGILIGYALYRDYQARDPLRSLGAYTLVANKFYLDDIYW
                     NGIVRPTRDDLSAGVYRFDQKVLDGIVNGTATFTKAVSRGVNWFDLKVIDGAVNGLAN
                     IAGFTGGLLRYIQSGNVQRYAVFLFTGVVVLAVIFTKI"
     gene            complement(2838..3137)
                     /gene="nuoK"
                     /locus_tag="VIH70_08595"
     CDS             complement(2838..3137)
                     /gene="nuoK"
                     /locus_tag="VIH70_08595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006517218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY8116458.1"
                     /translation="MRLPLVLFLSAALFSTGVYGVLARRNAVLVLMSIELMLNAVNIN
                     LVAFGAALHDLTGQVFALFVITVAAAEVGIGLAIVILIYRNRETINVDEVNLLKW"
     gene            complement(3137..3637)
                     /locus_tag="VIH70_08600"
     CDS             complement(3137..3637)
                     /locus_tag="VIH70_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY8116459.1"
                     /translation="MTGAEIAFVFIGVAAVVGALRVVTARNVVRAALYLVVTLSAVAG
                     VYLLLSAEFVAWVQILIYVGAIVILFLFGLMLTKAPIGREALDNQRRGVAFLVGLAIL
                     AGLVYLIQGAFDWDAARIDPLEGSVAAVGSSIFSRFVLPFEVISFLLLAALIGAIVLS
                     RKEDRP"
     gene            complement(3634..>4089)
                     /locus_tag="VIH70_08605"
     CDS             complement(3634..>4089)
                     /locus_tag="VIH70_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116460.1"
                     /translation="LTHEKEKLEEWTYSVLPPPFIEDGAEVPVEEPVSAPSVAPDTAG
                     PAAANAAAPSAGPAPAAASPATAQVAVGAPAVAAAAPAEAAASGEAAAPAEAAAAAPA
                     EHADVALDQETFDRVLAELLEQGTDRRVAEGRARRAAMVAARTKAEGGS"
BASE COUNT          802 a         1411 c         1339 g          537 t
ORIGIN      
        1 ccggccagca ggaccgagcc gatcgtcggc gcctcggtat gcgcgtcggg caaccacgtg
       61 tggaacggcc acatcggcac cttcacggcg aacccgagcc cgaccgccgc gaacacgatc
      121 agctggaagc cgtgagagaa accaccgctg gcggcgtagt cctggagcgc gatgatgtcg
      181 aaggtgtgga ccccgccgcc gacgtcggag ctgaagaaca tcccgaggaa ccccagcagc
      241 atgaacaccg aaccgaagag cgtgtacagg aagaacttga tcgcggcgta ctcacggttc
      301 gagctccccc agatcccgat gaggaagtac atcgggacga ggacgagctc ccagaacacg
      361 aagaacagga tcaggtcgaa cgcgatgaac gtgccggcca tcccggtctc gagcagcagc
      421 atgagcgcga tgaacgcctt cgtcttgccc ggtttcggga cgacccgcca cgtgtagatg
      481 gcgcagagga acgacagcac gaacgtcagc acgtacaggg gcaggctgat gccatcgatc
      541 ccgacgtggt agcgcgcgtc gatcgcgggg atccagctcg tgtcgagttg gaactgcagg
      601 cccgaggcgc cgtagtcgaa cgtgaacgtg atcgcgatcg cgaagacgag ggccgcaccc
      661 gtgaacacga tgccgagcgc gcgtacgcct ttctcgtact ggcgcggcac gagcgcgatc
      721 accagggctc ctgccaacgg caggagcgtc gcgatcgtga tcgccgagtt ctcccaggtc
      781 atgcggcttc cccttccatt ctcgggggct gacgctcgcg cgttcgcgct cgctccctga
      841 gcccccgcac ccccctcata tcttcgtgaa gatgacggcc agcaccacga cacccgtgaa
      901 caggaagacg gcgtaccgct ggacgttgcc cgactggatg tacctcaaca ggccgccggt
      961 gaaccctgcg atgttcgcca acccgttcac cgcaccgtcg atgaccttca ggtcgaacca
     1021 gttgacgccg cgcgagaccg ccttggtgaa cgtcgcggtc ccgttgacga tcccgtcgag
     1081 cactttctgg tcgaaccggt agacgcccgc agacaggtcg tcgcgggtgg gtcgcacgat
     1141 cccgttccag tagatgtcgt cgaggtagaa cttgttcgcg acgagcgtgt acgcgccgag
     1201 cgaccggagc ggatcgcgcg cctggtagtc gcgatacaag gcgtagccga tgaggatgcc
     1261 cccgagggcg gcgagcgtgg acaggacggc gacccacggc acgaacaggg cctcctcgag
     1321 ctcccggaag tacacccact tcccgaacac cgccccgagc tgcggcgacc ccacgaggcc
     1381 cgcaaccacc gtcacggagg ccagagccac gaggacgccg gtcatgctcc ggggtgcctc
     1441 gtgcggctcg ccgtggcctc ggtactcgcc gtagaaggtc atgagcacgg ccctggtcat
     1501 gtagaacgcg gtgaggagcg cggtcgccag gagcacgacg aacaggaccg ggtgaccctc
     1561 gtgctcggcg accacgagca actcgtcctt cgaccagaat ccggcgagcg gtggtacgcc
     1621 cgagagcgcc agggagccga tcaggaacgt ccagaacgtg atcggcaggc gtctacgcag
     1681 cccgcccatc tcgctcatgt tgttcgagtg cacggcgtgg atcacggcgc ccgacccgag
     1741 gaacaacagc gccttgaaga acgcgtgcgt gaacaggtgg aagaaggctc ccgtccggcc
     1801 ggccggaccc atcgacatgc cggcgatcat gtaggcgagc tggctgatgg tcgagtaggc
     1861 gagcacgcgc ttgatgtcgt cctgcgtgaa cgccatcagc gccgcgccga gcatcgtgat
     1921 gccggccacg atcgcgacga tctgcaggac gaacgggtcc gcatccagga acacgatgaa
     1981 caaccgtccc acgaggtaga cacccgccgc gaccatcgtg gccgcatgga tcagcgccga
     2041 gaccggcgtc ggccccgcca tggcatcggg gagccacacg tggagtggga actgggcgga
     2101 cttgccgatc gtgcctccga acaacagcag ggcggccgcc gaaaccagcc cgaccgaagc
     2161 gccgccgctc gacacgatct cggagatctt cgggatgttc gaggtcgtga acccggtgat
     2221 gaagatcagc acgaagatcc cgaacatgaa cggcacgtcg ccgacccggg tcgtgatgaa
     2281 cgccttgatc gccgaaccgg agttgacggg gtcctcccac cagtgcccga tcaggaggta
     2341 ggagcagacg cccataacct cccagcccac gaggagctgg aacaggttgt tcgccaggac
     2401 cacgacgagc atcgcgctcg tgaacaacga gagcacgacg tagaaccagg tgtagcgcac
     2461 gtcgcccttc atgtagccga gcgagtagat gtgcacgcag agcgagatcg aggtgacgac
     2521 gacgagcatc accgcggtca gaccgtcgac gtagaccccc acctccatgt ggagctgccc
     2581 gatctggaac caggggatcg atgcctcgta gaagccgcgc tcgccgccca cgaaggtcaa
     2641 cagggcgccg agcgacatga cgagggccgc ggcgagcgcg gtgatgccgt agaccgaacc
     2701 cttacccggc gtgcgcttgc cgatgaacag cgtgaacgcc gcggcggccg ccggcaggag
     2761 cgcgatcagc caggagttcc gcaggaacca gctcgggtcc tggatcgcct tgacgatgga
     2821 ctcggcggac tcggcggcta ccacttgagc aggttcacct cgtctacgtt gatcgtctcc
     2881 cggttgcggt agatcaggat cacgatcgcc aggccgatcc ccacctcggc ggccgccacc
     2941 gtgatcacga acagcgcgaa cacctgcccg gtgagatcgt ggagcgccgc cccgaacgcg
     3001 acgaggttga tgttgacggc gttcagcatc agctcgatcg acatcagcac caggaccgcg
     3061 ttgcgccgtg cgagcacgcc gtacacaccg gtcgagaaca gcgccgccga caggaagagc
     3121 accagcggca gcctgatcac gggcgatcct ccttccgcga cagcacgatc gccccgatca
     3181 gcgccgccag gagcaggaac gagatcacct cgaacggcag gacgaaccgg ctgaagatcg
     3241 aggacccgac cgcggccacc gaaccctcga gcgggtcgat ccgggcggcg tcccagtcga
     3301 acgccccctg gatcaggtag acgaggccgg cgaggatcgc gagcccgacc aggaacgcca
     3361 cgccacgccg ctggttgtcg agcgcctcgc gcccgatggg cgccttcgtg agcatcagtc
     3421 cgaacaggaa caggatcacg atcgcgccga cgtagatcag gatctgcacc cacgcgacga
     3481 actcggccga cagcagcagg tagaccccgg cgacggccga gagcgtcacc acgaggtata
     3541 gggcggcgcg cacgacgttc cgcgcggtca ccacgcggag ggcgccgacc acggcagcca
     3601 ccccgatgaa gacgaacgcg atctcggctc cggtcacgag ccgccctcgg ccttcgtccg
     3661 cgcggccacc atcgccgcac gccgcgcccg cccctcggcc acccgccgat cggtcccctg
     3721 ctccaacagc tccgccagca cccgatcgaa cgtctcctga tccagggcca cgtcggcgtg
     3781 ctcagcaggc gcggcagcag ccgcctcggc cggtgcagcg gcctcacccg atgctgcagc
     3841 ctcagccgga gcggcggcgg ccacggccgg agcgcccacc gcgacctgag cggtggcggg
     3901 cgacgcagcg gccggcgccg gacctgccga gggagccgcg gcgttcgccg cggcgggtcc
     3961 ggccgtgtcc ggcgccacgc tcggagccga gaccggctcc tccacgggca cctccgcgcc
     4021 gtcctcgatg aacggcggcg gcaggaccga gtacgtccac tcctcgagct tctccttctc
     4081 gtgcgtgag
//
LOCUS       DATBRK010000287         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_483211, whole genome shotgun sequence.
ACCESSION   DATBRK010000287 DATBRK010000000
VERSION     DATBRK010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483211"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VIH70_08610"
     CDS             <1..329
                     /locus_tag="VIH70_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY8116461.1"
                     /translation="RVECRIPGADANPYLAFAATIAAGLWGIENKVEPPPMFVGNAYE
                     AKDVPRVPYSLHEAVETLRGSTVAREAFGDFVFEHLLNTAYQEQVMFDNTTVTDWELA
                     RYFERG"
     gene            441..1559
                     /locus_tag="VIH70_08615"
     CDS             441..1559
                     /locus_tag="VIH70_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEY8116462.1"
                     /translation="MQHRSEEELIREIDAEHARLAASHRRLLGLLAHVDRRKSWRDVG
                     ARDTAHWLSIRYGLSQWKARRWIGAAHALETLPRTSEALASGELHVDKAVELTRFATP
                     ETESRLLVWAGGVSCGAIRRKADVVARRSSGEAEEADRSRFLSWWTFDEGRRFGMEAE
                     LPSAQGAVVAKVLDRLADGLPVMPQERGPAGAGARRADALVALCSSGDDADRDPDRAT
                     LVVHARLDGPDDELSECEIEDGPAISPDTARRLACNARVQTVVEEESGLPVRLGRMTR
                     EPPAWMVRHLKYRDGECRFPGCGSRRFTQAHHIVWWEHGGRTDLDNLVLVCTFHHRLV
                     HEYGWRVRREEDGTVSWFRREGRRYLPGPAPPEALVAS"
     gene            complement(1573..>3045)
                     /locus_tag="VIH70_08620"
     CDS             complement(1573..>3045)
                     /locus_tag="VIH70_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY8116463.1"
                     /translation="CALYAADGTLVASSYQAYDLSFPFPGAAEQDPNLWPAAVAAGVR
                     EVLAEVPEGPSAVKGLSFGSQLDGMVVCDAEARPLRPAMIWMDRRAEVQAAAVADRMP
                     PETFYGHVGANLDSSHAIFKALWVRDQEPGAWANAATLMSPGTYALREIAGPIAVDYS
                     NASSFALLDPRTRAWSPEVLAAVDVPESMLPEVVAATEPISTITTAFAEATGLDASTI
                     VAVGCGDEMAATLGAGVFEPGEVCDVVGTAEPVCAASSEPREDPTMLVECHPHADPDA
                     WLLENPGFVSGGNLRWWRDQFAPIERGAEAEGLGDAYDLLSREAERIPPGAEGLVFLP
                     CMQGAMAPEWNGAARGVFYGLTLAHSRAHMTRAILEGSAFALRDIVEAMANAGLDVRR
                     LTIVGGGAKGRLWRQIKADVTGLPVRVPTSVETTATGAAILAAVGARIHRTVGAAVEA
                     FVSYQPVEHQPDPDVREVYDEAYARYRKLYASLKPVFSDS"
BASE COUNT          454 a         1083 c         1076 g          432 t
ORIGIN      
        1 ctcgcgtcga gtgccggatc cccggggccg acgcgaaccc gtacctcgcg ttcgcggcga
       61 cgatcgccgc cggcctgtgg gggatcgaga acaaggtgga gccgccgccg atgttcgtcg
      121 gcaacgccta cgaggcgaag gacgtcccgc gcgtcccgta ctcgttgcac gaggcggtgg
      181 agacgctccg tgggtcgacg gtcgcgcgcg aagcgttcgg cgacttcgtg ttcgagcacc
      241 tgctgaacac ggcgtatcag gagcaggtca tgttcgacaa tacgaccgtc accgactggg
      301 agctcgcccg ctacttcgaa cgtgggtagg gcggcctctg ggccgatccc accgtctcgg
      361 atgccggtcg atatccttgg catccgaacg tatgttcgat aggctcctgg ttcctccccc
      421 cctgaccgtg cgaggtccgg gtgcaacacc gcagcgaaga ggagctgatc cgcgagatcg
      481 atgcggagca tgcgcgtctc gcggcgagcc atcgacggct gctcgggctc ctcgcgcacg
      541 tcgaccggcg aaagagctgg cgagacgtcg gcgctcgcga cacggcccac tggctctcga
      601 tcaggtacgg cctgtcccag tggaaggcgc gccgatggat cggcgccgct catgcgctcg
      661 agacgctgcc ccggacgtcc gaagcgctgg catcgggcga gctgcacgtc gacaaggccg
      721 tcgagctgac gaggttcgcc acgccggaga cggagtcccg cctgctggtc tgggcgggcg
      781 gcgtttcgtg cggcgcgatc cggcggaagg cggacgtcgt cgcacgcagg tcgtcgggcg
      841 aggccgagga ggcggatcga agccgcttcc tttcttggtg gaccttcgac gagggccgcc
      901 gcttcggcat ggaggccgag ctgccctccg cgcagggcgc ggtcgtcgcg aaggtgctcg
      961 atcggctcgc ggacggcctg ccggtcatgc cgcaagagcg cgggccggcc ggggcaggtg
     1021 cgcggcgggc cgatgccctc gtcgccctgt gttcgtccgg ggacgacgcc gatcgtgatc
     1081 ccgaccgtgc gacgctggtc gtgcacgcgc gcctcgatgg acccgatgac gagctgagcg
     1141 agtgcgagat cgaggacggc ccggcgatca gccctgacac ggcgcgtcgg ttggcgtgca
     1201 acgcgcgggt ccagaccgtc gtcgaagagg agagcggtct gcccgtcagg ctcggtcgca
     1261 tgaccagaga gcctccggcg tggatggttc gacacctgaa gtaccgggac ggcgagtgcc
     1321 ggttccccgg atgtggctct cgacggttca cgcaggccca tcacatcgtc tggtgggaac
     1381 acggcgggcg aacggatctc gacaacctgg tgctcgtctg cacgttccat caccgactcg
     1441 tccacgagta cggctggcgg gttcggcgcg aagaggacgg cacggtgagc tggttccgcc
     1501 gcgaggggcg tcgctaccta ccaggcccag cgccgccgga ggcgctcgtc gcctcgtagg
     1561 ggttgcccgc gctcagctgt ccgagaacac gggcttcagc gatgcataca gcttccggta
     1621 cctggcgtag gcctcgtcat acacctcgcg gacatcgggg tccggctggt gttccaccgg
     1681 ctgatacgag acgaaagcct ccacggcggc gccgacggtg cggtggatcc gcgcgccgac
     1741 ggcggccagg atcgcggcgc cggtcgccgt ggtctccacg ctcgtcggga cgcgcaccgg
     1801 aagacccgtg acgtccgcct tgatctgccg ccacagccgg cccttcgcgc cgccgcccac
     1861 gatcgtgagc cggcggacgt ccagacccgc gttcgccatc gcctcgacga tgtcgcggag
     1921 cgcgaacgcg ctgccctcca ggatcgcgcg cgtcatgtgc gcgcgagagt gcgcgagcgt
     1981 gagcccgtag aacacgccgc gcgcggcccc gttccactcg ggggccatcg ccccctgcat
     2041 gcacggcagg aacaccagtc cctcggcgcc gggggggatg cgctccgcct cccttgagag
     2101 gaggtcgtag gcgtcgccga gcccctcggc ctcggccccc cgctcgatcg gcgcgaactg
     2161 gtcgcgccac caccgaaggt tgccgcccga gacgaacccg gggttctcga gcaaccacgc
     2221 gtcggggtcg gcgtgcgggt ggcactcgac cagcatcgtc gggtcctcgc gcggctcact
     2281 cgacgccgcg cacaccggct cggccgtccc gacgacgtcg cacacctccc cgggctcgaa
     2341 cacgccggcc ccgagcgtcg ccgccatctc gtccccgcac ccgaccgcga cgatcgtcga
     2401 cgcgtccaga cccgtcgcct ccgcgaacgc cgtggtgatc gtcgagatcg gctccgtcgc
     2461 ggccacgacc tcggggagca tcgactcggg cacgtcgacg gccgcgagca cctcgggcga
     2521 ccacgcgcgc gtgcgagggt ccaggagcgc gaaggaggat gcgttcgagt agtcgacggc
     2581 gatgggaccc gcgatctcgc ggagcgcgta cgtcccgggc gacatcagcg tggccgcgtt
     2641 cgcccacgcc cccggctcct ggtcgcggac ccagagggcc ttgaagatcg cgtgcgagga
     2701 atccaggttc gcgccgacgt ggccgtagaa cgtctcgggc ggcatccgat ccgccaccgc
     2761 ggcagcctgc acctcggcgc gacgatccat ccagatcatg gccggtcgaa gagggcgagc
     2821 ctccgcgtcg cacaccacca tcccatccag ctgcgatccg aacgacagac ccttgaccgc
     2881 cgaagggccc tcgggaacct cggccaggac ctcccgcacc cccgcggcaa cggctgcggg
     2941 ccacaggttc gggtcctgct cggccgcgcc ggggaacggg aacgaaaggt cgtaggcctg
     3001 gtacgaggac gccaccagcg tcccgtccgc cgcgtagagc gcgca
//
LOCUS       DATBRK010000288        30126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_483654, whole genome shotgun sequence.
ACCESSION   DATBRK010000288 DATBRK010000000
VERSION     DATBRK010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 30126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30126
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483654"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VIH70_08625"
     CDS             <1..367
                     /locus_tag="VIH70_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY8116464.1"
                     /translation="TDLQPQMMALLASAEGTSIVTENVFEGRFMFVDELNRMGADIRT
                     EGHHAVIRGVDRLSAAPVRALDIRAGAAMVIAALMADGVTEIEDMYHVDRGYQDLEAK
                     LTALGAVVRRERARSPALQ"
     gene            556..2064
                     /locus_tag="VIH70_08630"
     CDS             556..2064
                     /locus_tag="VIH70_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116465.1"
                     /translation="MTLGGGLPTERGRGIGPRPEFLGVRWLDPAKRGGIERVATTIEE
                     TVTASSAHRVLVIVASTRPSADMRSSKDELYPLIVAEGLPGELFAGSGQEGSITSDST
                     HRDGIVTDLDVMPTIATFLGVDPPAEMTGSVIEIIPGPPPFELHERYLAQRRMYVPIG
                     TAGGIYLAIVGLGAIVLLALGRRVPEILRRVLGWGCLSVAALATGLLAAGHLPELTYA
                     TVIPFVALVTVFGTLAFAPLEHRDVLLIPAGIGIAVLAYFAVEAILSWAAALTPFVGG
                     SELDGGRFYGLPNVFIGLLIGASLWIAHRLRTVAGFGLLVGVALFAGLPDLGANLGGG
                     VSLFAAAGLWLAIRERERLGVWKGLGVFVGVTMLGTALILAAHAISPFETHVTRFEEQ
                     AGGLGGVLEKVWDRLNVGFDLIARNPFALIPVLGLPIAIAAVLRPPAALRPSLQRSPA
                     WRDAILVTLLAGVVAYLVNDSGPAAAGLAFGLGLGGLLGVSLLSEAGKMGEP"
     gene            2061..2867
                     /locus_tag="VIH70_08635"
     CDS             2061..2867
                     /locus_tag="VIH70_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY8116466.1"
                     /translation="MSSQPPIPPRPDGLHPVPVMPPRPDSLEVTDDLRPKASWSWYEA
                     LVVYIVAFLLAGLATLPLIRVMEPEADLTNIVLTVTAALVIMGVLLLWLSTYHKGWLE
                     VMRLPERGTWGREIGSGVLFGLGLYPVMVIVVGGLLTYLLQTISGEHVEAPEQVGEHL
                     PAIGVALTIVYAIVIAPIGEELFFRGVLFRSLRDRHGFWVGAVGSAIGFGLIHFIPGS
                     AIDAALLMIVMFLTGIALCFLYERRGTIVAPIAAHVTFNVIGIVLIYGLR"
     gene            2877..3296
                     /locus_tag="VIH70_08640"
     CDS             2877..3296
                     /locus_tag="VIH70_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY8116467.1"
                     /translation="MALFPSDEWLTRYRDLINGSQEYGEAAEDWEGDVAFFLEAEPDR
                     GVPEDLVAWLDLWHGECRGARMITADEGERSAYGIRAPYSRWRQVVLGELEPVKAMVQ
                     GKLKLRGDLANIVRHVRAAKELVHLTTLVPTEFLGSA"
     gene            3299..4336
                     /locus_tag="VIH70_08645"
     CDS             3299..4336
                     /locus_tag="VIH70_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8116468.1"
                     /translation="MRAVVYRGIERVEVADVPDPVIEDPGDAVVRVGLTAICGSDLHL
                     YHGKAPLEPGDTIGHEAIGVIEDVGPAVTRFAPGQRVVVSFDIACGACWFCRKGQTQL
                     CEDFGFLGYGMFGGSLGGAQAELLRVPVADVNLLAVPEGLEDDRALFVGDALTTGVYA
                     AALAGVTDGDTVAVVGAGPVGFFCAQAALPNASSVLALDLDHARLALAGRTGAIPIDV
                     GARNAQTAVFEHTDGRGADVVIEAVGTPAAFERSLDIVRRGGTVVVMGVYTSEIVQAQ
                     IGVWWIRALQLRFAGTTPVHTWWERAMAEVQAGRLDPRPIVSHRLPLEEAPKGYELFD
                     TKQATKVLLEP"
     gene            4359..4772
                     /locus_tag="VIH70_08650"
     CDS             4359..4772
                     /locus_tag="VIH70_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116469.1"
                     /translation="MTVRFADAEPNGLADLVGRLIQTNFEAEPDRRALLLDAVVLLTA
                     TDAAMEATVTLSSAGVAVSNGSAGRRPHLVIAADAYDLIELAGAPLRFGFPDVLNPQG
                     RSVIRRIATRHVRVSGMVRHPIRLSRFTRLLSVVP"
     gene            4769..5350
                     /locus_tag="VIH70_08655"
     CDS             4769..5350
                     /locus_tag="VIH70_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116470.1"
                     /translation="MSAGAEPAPLPAPPLRQRRWIPVIALTAVLAAVTSGGYLTSDAL
                     GEVRGGAVAVGTSVRVTPFPGWELAGRSTDPDRIRLTSGSTSLDVAAFPFDGTDVDLL
                     GRYVDNILEPDAEQFRVSEEVEPVRLASGLTGSRIAYVGLFGDVQAPIEGEVTAVVSP
                     TGQGVIFDGWAPAGQLQFEIDEIDAMIERAEIA"
     gene            5350..6588
                     /locus_tag="VIH70_08660"
     CDS             5350..6588
                     /locus_tag="VIH70_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEY8116471.1"
                     /translation="MSERRGGRLAFEPQIVRMPRNWGRQTSLFQWRQPAFWLYAAIVV
                     VAAVYTVSQQQLFQKLSPSGWALSWGLLLLYGLPLFLAIYLLDLYEREPMSLVLGALV
                     WGGVAATVLAGIANEGWGLVVARLGGPVFAAEWTAALTAPFVEETVKVAGVVLIYLIA
                     RDEMDDMMDGFVYGAMVGLGFTLVEDVFYVIAIFGGSPGGVLAGFYVRVVSSGFYGHV
                     LYTGLSGMGVAYFVSRRGEVAFGRRLAVALGLFGSAVAAHFLWNSPFLNFFPSSVDSV
                     GDWLRIPFAAAVKGAPLLLFVVVMVRLARRRERMWLETALRSEVDSPWLSEVELRVLL
                     DPSARRRSRREMRARAGDAAARLLKRLQQEQINLAMVRARAASEDGPELVRQRALCAS
                     LRDALLAMPGAALAGTRREG"
     gene            6688..7623
                     /gene="meaB"
                     /locus_tag="VIH70_08665"
     CDS             6688..7623
                     /gene="meaB"
                     /locus_tag="VIH70_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017610365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY8116472.1"
                     /translation="MSLTDDVLAGDPRAVARAISMVEDGAAGLEALSAELFPRTGRAF
                     TVGLTGSPGVGKSTIVGELVGAARAQGRSVAVLAIDPTSPLSGGALLGDRLRMQAHAT
                     DPGVFIRSMATRGHLGGMALAAPEAIRVLDAAGHDLVIVETVGVGQAEVDVAAATDTT
                     VVVVSPGWGDAVQVAKAGILEIADVFVVNKADREGSEVAVRDLQQMLRMGPELPWTPP
                     VVPTTATEGTGIDDLWAALELHRAHLASSGDLERGRRDRLLREVESLSLEKLRGRARD
                     LLTNDGSLATDLTERRTDPYRTAAILEERITTQGS"
     gene            7683..9326
                     /locus_tag="VIH70_08670"
     CDS             7683..9326
                     /locus_tag="VIH70_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8116473.1"
                     /translation="MVEQRERRSAEDTQPSGARTLSGEPLEPLYGPADLAGFDPEAKL
                     GRPGAYPFTRGVYPSMYRGRLWTMRQFAGFGSAPETNARYRFLLERGQGGLSVAFDMP
                     TLMGRDSDDPLAEGEVGRCGVATDSLDDFEALFDSIPLGDISTSMTISGPAVVVFAFF
                     LAAAERQGVPWERLDGTLQTDILKEYIAQKEWIFPPRPHLRLIGDLMEFCLERVPRWH
                     PISVSGYHIREAGANAWQELAYTLADGFAYVELGLDRGLDVDRFAPGLSFFFNAHVDF
                     FEEIAKYRAARRIWARWLRERYGAKDESSMRLRFHTQTAGVSLTAQQPMNNVVRTAIE
                     ALAAVLGGTQSLHTNALDEVLALPTEEAAKLALRTQQIIAHETGVPDVIDPLGGSYFL
                     ETLTDRMEELAEAEFARILAMGDGSMLEGVLTGIERGVFQQAIAESAFSEQERFERGD
                     LVKVGVTDFVEAGEQPIEILEISGGVETSQVERVRRVRERRDAAAARAALDRLGELAA
                     TDANLVEPLVDCARALCTEGEIVDALRSVFGSYTETPRF"
     gene            9427..10320
                     /locus_tag="VIH70_08675"
     CDS             9427..10320
                     /locus_tag="VIH70_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116474.1"
                     /translation="MLAASEALGVGVIVSAFLFGFRHGIDWDHIAAITDITSSQEDRG
                     PALEYGTIYALGHALVVFAIGVLAIVLGAKLPDGVDAVMERVVGVTLILLGVYVFSAL
                     VRHGREFRMRSRWMLIFSGVHGAFRKVRGGRRREAHDLEPVHRHAEQDGSLSVAVAED
                     IPVSEWHHGHHGRPGHHHHKHPEPTDEAFMNYGRGTAFGVGMLHGIGAETPTQVVIFL
                     TAAGAGGALAGVLILVVFLIGLLASNTLITFGSAFGFVTASKNWTLYVTVAVLTASAS
                     LIIGTLFLLGKGAVLPALFGG"
     gene            complement(10324..10779)
                     /locus_tag="VIH70_08680"
     CDS             complement(10324..10779)
                     /locus_tag="VIH70_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY8116475.1"
                     /translation="MGQTAEQASNVDVISALRERGLRMTPQRRAIVAEIMRTQGHISP
                     TALARKVQGDMPGVNASTIYRTLALLEEMGVLAHAHLESGAEYHRTEEAGHVHLTCSN
                     CGDEDDLSIGEADALIRLIEQHHGFEPDLTHFAIGGLCADCRKASGAAG"
     gene            10866..11636
                     /locus_tag="VIH70_08685"
     CDS             10866..11636
                     /locus_tag="VIH70_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018802578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8116476.1"
                     /translation="MGEFVRIDTEGGVATIRIDRPPANALARPVSLELSEAAATVAGD
                     EAVRAVVVWGGERIFAAGADIRKMVGYGPDEIASDVGALEQACRDLEAIPKVTIAAIN
                     GFALGGGLEVALSCDVRFIAEDARLGLPEIKLGIIPGSGGTQRLPRLVGLAKARHLIL
                     TGRQVDAAEALAIGLVDRLAAPDEVYRVAVDEARTFAAGPLLAYAAAKRAMAAADHPL
                     EEGLRVEREAFVALFATRDQEEGMRAFLEKREPKFEGR"
     gene            11638..12129
                     /locus_tag="VIH70_08690"
     CDS             11638..12129
                     /locus_tag="VIH70_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8116477.1"
                     /translation="MRVRQATVEDVPSLVALFQELDRMQADWRVFTPRPGFYDEVGAK
                     YNEALADPDTVVLVAEDEDGEVVGMAYGEARTPSRFSDERALELSGVVVRAGYRGRGV
                     GRELVHEAGRFAADRGVRWVELKTFAPNRGAMEFWEDLGFTARVVQLTSPTKVLLERL
                     EGR"
     gene            12252..12461
                     /locus_tag="VIH70_08695"
     CDS             12252..12461
                     /locus_tag="VIH70_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116478.1"
                     /translation="MSDSAYRGARIRELPRANRTYPGGRVCAAEGCSTKLSIYNRWDL
                     CWQHEPVHTYISRGKRRSRKEMEAA"
     gene            complement(12533..12772)
                     /locus_tag="VIH70_08700"
     CDS             complement(12533..12772)
                     /locus_tag="VIH70_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116479.1"
                     /translation="MSEDVHTVHLGAFTHEHAEAIAGELEKAGIVWWYKNPGFFSQIW
                     ERSVRLFVDRNRLEEAQAIADRVLASGRPEGDGSS"
     gene            complement(12769..14541)
                     /gene="pabB"
                     /locus_tag="VIH70_08705"
     CDS             complement(12769..14541)
                     /gene="pabB"
                     /locus_tag="VIH70_08705"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY8116480.1"
                     /translation="MIEARFDDLVAGAERAFRLAEPVGVIEARRPGEVAGAIDAAGSA
                     AARGLWAAGFVAYEAAPGLDPELSVHVRAPDDPFAELPLAWFALFERKEDLPPLEPGR
                     LDPSGSEGSPWRPSVDRAIYDAAVERIRELIAAGHTYQVNHTIRLRAMIQGDERGFYR
                     DLCLAQRGGYAAFLDVGRFRVLSASPELFFRIDGQRITTKPMKGTAPRGRWPAEDEEI
                     AARLVASSKERAENAMIVDLLRNDLGRICRTGSVEVERMVEAERFETVWQLTSTIEGD
                     LRPEITLLDAFRALFPSGSVTGAPKVRTTRIIADLEDSARGPYCGAIGYLSPPGSGEP
                     RANFNVAIRTVVLDTQTRTAEFGVGGGITHDSSASGEYEEILAKARVLSDVRPAFELF
                     ESLAHVPAEGFRHLNEHLERLAASARYFGFRFELEEAAATLKQAVDDVTEPSVVRLTL
                     ARDGALSTDVRDLPPAEERVRVSLDDEPVDPSDVWLFHKTTRRGPYERRRDRRPDVND
                     VVLVNDRGEVTESTVANLAVRLEGVWVTPPIDAGLLPGTYRTVLLREGRLIERPVTVD
                     GLRGAGELALVSSVRGWRPAVLVP"
     gene            complement(14554..15513)
                     /locus_tag="VIH70_08710"
     CDS             complement(14554..15513)
                     /locus_tag="VIH70_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY8116481.1"
                     /translation="MTTVWVYADITPEGPSPSALELLTKARTIGDEVAAVALGPGARA
                     AAITLGEYGAARVFASDEQVYADTLGRPAAHTLHALIQEHTPDLVLFSTSYDARDVAG
                     RLQARLGSTLMSNVTDVTGAGSAQTQIFGGSRVVDVALDGPTPRLVLVRPKSFAPEPN
                     GGTAEVVPVDTPVPDEFTTVRRIERHEETAAGPRLEDAKVVIAGGRGLQDPSNFKLLD
                     DLAAAIGDAAVGASRAVVDAGWVPYSYQVGQTGKTVKPEVYIAVGISGATQHVVGMKG
                     AKRIIAINKDGDAPIFRLADLGVVGDALTVVPKVIEEIRARRG"
     gene            complement(15515..16282)
                     /locus_tag="VIH70_08715"
     CDS             complement(15515..16282)
                     /locus_tag="VIH70_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY8116482.1"
                     /translation="MHVVVLVKYVPEPQGTPTLGEDLLLVREGVDGALDPGDEFAVEA
                     ALRLVEMHGGEVTLVSMGPEVAAGALRRGFSMGAGGGVLVTDPALRGADALATARVLA
                     AAVVRAPFDLVLAGVESTDGSTGTLPMTVAELLGIPSATFARKVEVEEGALRMERQTE
                     LGYDVVTSPLPALVTVTGSAAEPRYPTLKGIMAAKSKPLEQLSLADLGLFDADVAASQ
                     RVTSVSEAPEKSAGVVIQAGDDAASKIAELLADAKAI"
     gene            16369..17439
                     /gene="mnmA"
                     /locus_tag="VIH70_08720"
     CDS             16369..17439
                     /gene="mnmA"
                     /locus_tag="VIH70_08720"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY8116483.1"
                     /translation="MSKGTVLVAMSGGVDSSVAACLLLEQGYEVVGSHMRLVHMDGVE
                     HGCCGPTARNDAAEVARIAGFPFEICDLSDEFERTVIGDFVAEHEAGRTPNPCARCNG
                     EIKFGAFLRRADELGIDAVATGHYVRTDRIGGRLRLLRGADRAKDQSYMLHMLGQREL
                     SRSRFPVGGLPKAETRAAAERYGLPVASKPDSQELCFAPSGQAGAYLVSVAPHLMNEG
                     EVVDTEGRVLAPHAGSATFTIGQRRGLGVSGPEPSYVLEIDARANRVVVGPGELLARS
                     GAIADRVSWVAGELPSRVPFEAEVRIRYRGDDVPAVVEPLGDTVRIEFRTPQRGVAPG
                     QSAVVYRDEELLGGGRIVDALR"
     gene            17524..18201
                     /locus_tag="VIH70_08725"
     CDS             17524..18201
                     /locus_tag="VIH70_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116484.1"
                     /translation="MRLLKRKSRTFEIQVQDMTLHIQAATDVDEESRAAALSFWELLH
                     TYSIRNPEFRSSKRPVKVPGDAPEIVREIASTAARAGVGPMFSLQGAVTDHVGRFLAH
                     EVREVTVNCGGDYFIRSRRRQKLTVLRRPGSSVAVVVPPNREGLGVSMTLGHGPRRSE
                     VDGLAVLAESCMLAGAAAAGVQAILTKEDGLHGALTYLKQVPGVIGGVVFLGERIGMA
                     GGLELAA"
     gene            18215..20254
                     /gene="ligA"
                     /locus_tag="VIH70_08730"
     CDS             18215..20254
                     /gene="ligA"
                     /locus_tag="VIH70_08730"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY8116485.1"
                     /translation="MSTTSEERELANAKLRVEELREQANFHSYRYHVLDDPEVSDAEY
                     DDLMKELRAFEERFPELVTPDSPTQRVGATPADLFAPVEHRTPMLSLDNAFSREELDA
                     WATRVERGVGSGAQYSCELKIDGVACALTYENGALTKGATRGDGRTGEDITANVRTVR
                     SVPQRLDVKVPPAYLEVRGEIYLPVRAFERLNEELLEAEKRPFANPRNAAAGSLRQKD
                     PKVSASRPLALLVHSFGFADGIRFDSHSGFLDWSREARLPVASTSEVVKDVDDVVAYL
                     QRWEADRHSVPWEIDGVVVKVDQTALQQELGATSHAPRWAIAYKFPPEERTTLLREIG
                     VHTGRTGIVTPFAFLEPVQVAGVTISTATLHNEDEVRRKDVREKDTVIVRRAGDVIPE
                     VVGPVLSKRRKGTRRWTFPTTCPSCGTVLVRKEGEAYRRCPNKRECPSQNIEWLFSFA
                     SRGGLDIEGLGYKTGIALIDLGLVTDPGDVFSLTADDLSQLPGFADKSISNLMSSIEA
                     GKDRPIWRLLTALNIPHVGTHVAQVLSNAFGSIDALLKASEQDIDDVEEIGPEIARSV
                     HTWFADKGNRRLLEKLRAAGVRMKDVAPRKPKGKRPLAGKTIVLTGGLQGLSRDEAAA
                     AAEAAGARVVSSVSKKTDFVVVGENPGTKAARAEELGVETIDEREFAKRLGRKQA"
     gene            20314..21231
                     /locus_tag="VIH70_08735"
     CDS             20314..21231
                     /locus_tag="VIH70_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEY8116486.1"
                     /translation="MTAVVIDGNAIAEQLRQSIADELQVLRLSEVRPGLATVLIGESF
                     DAQAYERHVKRLAESLDYRYVCESLSADVELADVIATLGKLNADPRITGILVLHPLPP
                     HIPEAEMNSAVDPRKDIEAVHPMNAGLLAQGRPRFLPSTPASCFYLLDHYADAHGGGH
                     ESFYSGRTLVVVGRSNSVGKPAQMLGLQRDATVIACHSRTKPLEAYTRQADILIVAAG
                     VRGIVDGSMVQEGVVAVDVGIHAVKDEETGKTRMVGDLNFESVAEKAEAITPVPGGVG
                     PITDVWLVGNALMAAAIAHQVEPRFGLFR"
     gene            21401..21691
                     /gene="gatC"
                     /locus_tag="VIH70_08740"
     CDS             21401..21691
                     /gene="gatC"
                     /locus_tag="VIH70_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006285158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY8116487.1"
                     /translation="MPVEIDIEHVARLARLELTQDEKARLREQLGVILEAAAKVSEVA
                     TDDVPPTAYAIPRSNVLRPDEITPSLTVEEVLSNAPEVEDDRFKVPKVVEVE"
     gene            21688..23160
                     /gene="gatA"
                     /locus_tag="VIH70_08745"
     CDS             21688..23160
                     /gene="gatA"
                     /locus_tag="VIH70_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY8116488.1"
                     /translation="MTELCDRTASDLASALRAGECSAAEILESSTERIDDVDGRVGAF
                     LARTDELAREQARTADERIAAGDAPPAAGIPLALKDVLSTRGVTTTCGSRILETYVPP
                     FDCTPWERLERDGGVLVGKTNCDEFAMGSSNENSAFGPVHNPWDLDLVPGGSSGGSAA
                     AVAAGEAVWALGTDTGGSVRQPASLSGVVGLKPTYGLISRYGLIAFASSLDTVGTLTR
                     SVRDAALLLSAIGGKDPMDATSLDEPRRDYTEGLDGEISGIRVGVVKEAFGEGVEPGV
                     RDSVRHAVDELANLGARVGEASFPHADYALSAYYLIAPSEASSNLARYDGVRYGLRVD
                     GADSVEMMTRTRGAGFGAEVKRRIMLGTYALSAGYYEAYYGQAQKVRTLIIRDYEAAF
                     GAFDVLVSPTSPTTAFPIGAKIDDPLAMYLNDVFTIPANLSGMPAISIPSGVDGAGLP
                     VGLQFTAPVLAEPTLLRVANALEGALGFDARPPLLAGSPA"
     gene            complement(23509..24483)
                     /locus_tag="VIH70_08750"
     CDS             complement(23509..24483)
                     /locus_tag="VIH70_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8116489.1"
                     /translation="MSAPIEQPTARGNGSSAAIWTALSLVYVAWSTTFVAIRVVNETM
                     PPLLGGGLRFLIAGGLVLPIALRFGDRRGDRPRATQWRGAAVVAALMMFGGNGGIVWA
                     ERTIPSGMAGLAIATVPLWMATIDRVVLGHRQPRVVVIGLAVGFIGAALLVGGNALEG
                     EIDMGGLVLALGAAASWAAGSIYQRHAALPRRPLVAAGMEMLVAAGLFFVVGGLVGEF
                     GDLEPGSTSRASWIALGYLVVVGSWIGFTCYLWLLRNARTSLVATYAYVTPIGAVIVG
                     ALILDETFTASTLLAGVLIVAAVALIVSAGSARGEPERPAEELEEAAA"
     gene            24635..26092
                     /gene="gatB"
                     /locus_tag="VIH70_08755"
     CDS             24635..26092
                     /gene="gatB"
                     /locus_tag="VIH70_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY8116490.1"
                     /translation="MADAFETVIGLECHIELSTDSKMFCGCRNAFGAPPNTNVCPVCL
                     GLPGSLPVPNEKAIESIVRIGLALGSTIAPDSLFHRKNYFYPDMPKDYQISQYDLPVC
                     VGGRLEIEVDGERRTIGVTRVHMEEDTGKTTHVGAGGRIGQAEYALVDYNRAGVPLVE
                     CVSEPDLRSAEEARAYFVGLRATLEALDVSDVRMEEGSLRCDANISTRRTGETELGTK
                     VEIKNLNSVRSLERALRYEEERQRTALERGEPLVQETRHFDEETGATHTLRSKEEAFD
                     YRYFPEPDLTPLEPDRAWVKVLEAELPELPAARRERYVDGLGLRPEQARILAGSPSAA
                     GFFEATIALGADPASAANWITQDLAGLVNTARIELADARVTPRHVADLVALVADETIS
                     ATGAKQALEEAFETGDAAEAIVERRGLRQVVDASALEAWVDEAIAENPGPVEQFRGGK
                     EGALNAVLGQVMKKSGGSASPKAARELLLQRLTGS"
     gene            26104..27360
                     /locus_tag="VIH70_08760"
     CDS             26104..27360
                     /locus_tag="VIH70_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116491.1"
                     /translation="MRVDRRAPKRYPWWQLILLGLVAMVEALFRPLARMWRSEDPLDT
                     YGLAHLGSVAGDAMVAIALAGSVFFEIPPPEAEVKVAVFLALTIAPLAVAGPFLVPLL
                     DRAGPRRAISFGAAAARAVVCIYAAPRFGTLLLFPCAFLLLVLSKVHAITKNGLAVAY
                     AGPNEGLVRANARLGRIATGGAVLAAGPAFALLKLGAAEAVLYGAALVYGIAAALNLR
                     LPHPRVRGGQAAVSKLGSIPQLTAPAIGAAGLRAASGFLLFALAFALRRSGEPTWWFA
                     VLAGAATAGAYLGDLIVPRLPANLREEAVVIGCLMGAGVGATLSFFAFELVVLVLFAI
                     VVGASSELGRLAFQTLMQRLAPGGSYGRVFVRYEILFQLAWVGGALIPALAPIDFRDG
                     FLILAALYLVAIIGYLTPDILARRRG"
     gene            27454..27771
                     /locus_tag="VIH70_08765"
     CDS             27454..27771
                     /locus_tag="VIH70_08765"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY8116492.1"
                     /translation="MAEWVRVGSAGSVPEGEITAFAAGERQVAVANVEGDLHAFDDTC
                     THQQCSLAEGELDGTTVECACHGSLFDVITGEAIGGPAVDPVGVFEVKEEDGELKVLV
                     DEE"
     gene            27768..28988
                     /locus_tag="VIH70_08770"
     CDS             27768..28988
                     /locus_tag="VIH70_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8116493.1"
                     /translation="MTAFVVVGAGVAGATAAAKVREEGFDGDLVLIGAEPMPPYERPP
                     LSKEYLRGESDHHFVRPPEWYAEHQIDARFGAKAERVNVSDRVVELEGGERVPFDQVV
                     IATGARNRRPKIDGLDLPGVFDLRFAPDADAIKRAAKEGSKAVMVGMGFIGAEVAASL
                     RQLGLEVTVVEFFGTPLERVLGPTLGGAIESLHRDHGVEMIFRDGAERFEGDGRFEAM
                     ITKEGRRIEGDFAVVGVGVEPVTEVADGGVPVDNGIPVDANLRTSVDGVWAIGDVARH
                     DHPVFGPIRVEHFDNALKMGEHVAKNLLGTPAAFDDAHWFWSDQYDAEIQMAGFATEW
                     DDMVVRGSIEDRSFSAFLLKDGRLLSTFSMNRKFDVRRSMPLIAAKAHPDSRQLADPE
                     FDLRQLHPPKEQAR"
     gene            28985..29206
                     /locus_tag="VIH70_08775"
     CDS             28985..29206
                     /locus_tag="VIH70_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116494.1"
                     /translation="MMQPNITHMPCDLNQQQKRHDARKDSLARAVAWRMGKRSWLDRL
                     LRRGTPGLELSAEDLRPRETEDAFHGFRV"
     gene            complement(29246..29458)
                     /locus_tag="VIH70_08780"
     CDS             complement(29246..29458)
                     /locus_tag="VIH70_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116495.1"
                     /translation="MTDRQGTVAALGESTLTGWRGAVGNAVAKPVAKRTRFTEEQIRV
                     ALGLALLAYGLYRVLWPPIRALMRSR"
     gene            complement(29497..>30126)
                     /locus_tag="VIH70_08785"
     CDS             complement(29497..>30126)
                     /locus_tag="VIH70_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8116496.1"
                     /translation="ISLAPKDFVALMGPSGSGKTTFLGVLSGLDAPDAGEVRWKDRPI
                     RKLSFDEQIALRRSGMGFVFQSFGLLPTLSAVENVELPLRVSGTDLKESKARAEMWLA
                     RLGLGDRMYNRTFELSAGQQQRVAVARALIGEPEVVLADEPIAEVDTENADLILEALW
                     EVIRRGGAVLAATHNPEVLRYSSRVILFRDGKVEAEGRPEEISARLTVD"
BASE COUNT         4228 a        10446 c        10927 g         4525 t
ORIGIN      
        1 gaccgacctc cagccgcaga tgatggcgct cctcgcgtcg gcggagggaa cgagcatcgt
       61 gaccgagaac gtgttcgagg gccggttcat gttcgtggac gagctgaacc ggatgggcgc
      121 ggatatccgc accgagggcc atcatgccgt gatccgcggc gtcgaccgac tctcggccgc
      181 gcccgtgcga gcgctcgaca tccgcgccgg cgccgcgatg gtgatcgcgg ccctgatggc
      241 cgacggcgtg accgagatcg aagacatgta ccacgtcgac cgcggctatc aggacctcga
      301 ggccaagctg acggcgctcg gcgccgtcgt ccgtcgcgag cgcgcacgct cacccgccct
      361 ccagtgaccc gcgcgcgtct cggcgcgctc gccgtctgcg ctctcgttgg gttcggtctc
      421 gggaccgctc cggcccgcgc tcaggagcca cgcggacgcg aggcatatct cttcctcgtc
      481 ccagggatct cgttcgagga cgcaatggcc gatggcacgc tggcatcgct cgccacggat
      541 ggcggagccg ggctgatgac cctgggaggc ggcctgccga ccgagcgggg acgggggatc
      601 ggtccccggc cggagttcct cggcgttcgt tggctcgacc cggcgaagcg agggggcatc
      661 gagcgcgtcg cgacgacgat cgaagagacg gtgacggcat ccagcgcgca ccgtgtcctg
      721 gtgatcgtgg cctcgaccag gccttcggcc gacatgcggt cgtccaagga tgagctctat
      781 ccgctgatcg tcgccgaagg ccttccgggc gagctgttcg cgggcagcgg gcaggaggga
      841 tcgatcacga gcgactcgac gcatcgggat ggcatcgtga ccgacctcga cgtgatgccg
      901 acgatcgcga ccttcctggg agtcgatcca cctgccgaga tgaccggctc ggtgatcgag
      961 atcatcccgg ggcctccgcc gttcgagctc cacgagcgct acctcgccca acgccggatg
     1021 tacgtcccga tcggaaccgc cggcggcatc tatctcgcga tcgtcgggct gggcgcgatc
     1081 gtgctcctcg cgctcgggcg ccgtgtgccg gagatccttc gtcgggtcct cgggtggggg
     1141 tgcctgtccg tcgccgcact cgcgaccggc ctgctcgccg ccggccatct gcccgagctc
     1201 acgtacgcga cggtgattcc gttcgtcgca ctggtgacgg tgttcgggac gctggctttc
     1261 gcgccgctcg agcaccggga cgtcctgctg atcccggccg gtatcgggat cgccgtcctc
     1321 gcgtacttcg ccgtcgaggc gatcctcagc tgggcggccg cgctcacccc gttcgtcgga
     1381 ggctccgagc tcgacggcgg ccgcttctac ggcttgccga acgtcttcat cggcctcctg
     1441 atcggcgcgt ccctgtggat cgcgcatcgg ctccggaccg tcgccggctt cggcctgctc
     1501 gtgggcgtcg ctctgttcgc cgggctgccc gacctcggcg cgaacctggg cggtggggtg
     1561 tcgttgttcg cggcggccgg cctatggctg gcgatccgcg aacgagaacg gctcggcgtg
     1621 tggaagggtc tcggagtgtt cgtgggcgtg accatgctcg ggacggcctt gatcctcgcg
     1681 gcccacgcga tctcgccgtt cgagacgcac gtcacgaggt tcgaggagca ggcgggcggg
     1741 ctcgggggcg tgctcgaaaa ggtctgggac cggctcaacg tcggcttcga cctgatcgcg
     1801 cggaacccgt tcgcgctgat cccggtgctc ggcctgccga tcgcgatcgc ggcggtgctc
     1861 cgaccgccgg ccgcgcttcg gccgagcctg cagcggagcc ccgcatggcg ggacgcgatc
     1921 ctcgtgacgt tgctggccgg cgtcgtcgcc tacctcgtga acgattcggg gccggcggcg
     1981 gccggcttgg cgttcgggct gggcctgggc ggcctcctcg gcgtgtcgct cctgtccgaa
     2041 gccgggaaga tgggggagcc gtgagcagcc agccgccgat cccaccgcgc cccgacgggc
     2101 tccatccggt gccggtgatg cccccgcgtc ccgactccct cgaggtgacc gacgacctgc
     2161 ggcccaaggc gtcctggagc tggtacgagg cgctcgtcgt ctacatcgtg gcgttcctgc
     2221 tggccgggct cgcgacactg ccgctgatcc gggtgatgga gccggaggcc gatctgacga
     2281 acatcgtcct gacggtcacc gccgcgctcg tgatcatggg cgtcctgctc ctgtggctct
     2341 cgacctacca caagggatgg ctcgaggtga tgcgcctccc ggaacgcgga acctggggga
     2401 gggagatcgg gtcgggcgtc ctgttcggtc tcgggctgta tcccgtgatg gtgatcgtcg
     2461 tcggcggcct gctcacctac ctcctgcaga cgataagcgg cgagcacgtc gaggcccccg
     2521 agcaggtggg cgagcacctc ccggcgatcg gggtcgcgct gacgatcgtc tacgcgatcg
     2581 tgatcgcccc gatcggcgag gagctgttct tccgaggcgt gctgttccgg tcgctgcgcg
     2641 atcggcatgg gttctgggtg ggggcggtgg gctcggcgat cggcttcggg ctgatccact
     2701 tcatccccgg ttcggcgatc gacgcggcgc tgctcatgat cgtgatgttc ctcaccggca
     2761 tcgccctgtg cttcctgtac gagcggcgcg ggacgatcgt ggctccgatc gccgcccacg
     2821 tcaccttcaa cgtgatcggc atcgtgctga tctacgggtt gcggtgagga cgaccgatgg
     2881 ccctgttccc gagcgacgaa tggctgacgc ggtaccggga cctcatcaac ggttcccagg
     2941 agtacggcga ggcggcggag gactgggagg gcgacgtcgc gttcttcctt gaggcagagc
     3001 ccgatcgcgg cgtgcccgag gatctcgtgg cctggctcga cctgtggcac ggcgagtgtc
     3061 gcggcgcaag gatgatcacc gcggacgagg gcgagagatc agcgtacggg atccgagcgc
     3121 cgtactcgcg ctggcgccaa gtggtcctgg gcgagctcga acccgtcaag gcgatggtgc
     3181 agggaaagct caagctgcgc ggcgatctgg ccaacatcgt tcggcacgtt cgtgccgcga
     3241 aggagctcgt gcacctcacg acgctcgtcc cgaccgagtt cctcggcagc gcctgacgat
     3301 gcgggccgtc gtctaccgcg gcatcgaacg cgtcgaggtc gccgacgtcc ccgatccggt
     3361 catcgaggac ccgggcgacg cggtcgtgcg cgtcgggctg acggcgatct gcgggtccga
     3421 cctgcacctg taccacggca aggcgccgct cgagcccggc gacacgatcg gccacgaggc
     3481 gatcggcgtg atcgaggacg tcggccccgc cgtcacccgg ttcgcgcccg gccaaagggt
     3541 ggtggtctcg ttcgacatcg cgtgcggcgc gtgctggttc tgccggaagg ggcagacgca
     3601 gctctgcgag gacttcggct tcctcggcta cgggatgttc ggcgggagcc tcggcggcgc
     3661 gcaggccgag ctgctccggg tccccgtggc cgacgtcaac ctcctcgccg tgcccgaggg
     3721 tctcgaggac gatcgcgcgc tcttcgtcgg cgacgccctg acgaccggcg tctacgcggc
     3781 cgcgttggcc ggtgtcaccg acggcgacac ggtcgcggtc gtcggggccg gaccggtcgg
     3841 gtttttctgc gcgcaggcgg cgctcccgaa cgcgtcctcg gtgcttgccc tcgacctcga
     3901 ccacgccagg ctcgcgctcg ccggacgcac gggcgcgatc ccgatcgacg tcggggcacg
     3961 caacgcgcag accgcggtgt tcgaacacac ggacggacgg ggcgcggacg tcgtgatcga
     4021 ggccgtcggg acgcccgcgg cgttcgagcg ctcgctcgac atcgtgcggc ggggcggaac
     4081 cgtcgtggtg atgggcgtct acacgagcga gatcgtccag gcgcagatcg gcgtgtggtg
     4141 gatccgcgcg ctccagctcc ggttcgccgg gacgacgccg gtccacacct ggtgggagcg
     4201 cgcgatggcc gaggtgcagg ccggtcggct cgacccccgg ccgatcgtct cgcaccggct
     4261 gccgctcgag gaggccccga agggctacga gctgttcgac acgaagcagg cgacgaaggt
     4321 cctcctggag ccgtgaccgt cccggtctcg gtcgctccgt gacggtgcgg ttcgccgacg
     4381 ccgagccgaa cggactggcc gacctcgtcg gccggctcat ccagaccaac ttcgaggccg
     4441 aacccgatcg ccgcgccctc ctcctcgacg cggtcgtgct cttgacggcc accgacgccg
     4501 cgatggaggc gacggtcacc ctttcgagcg ccggggtcgc cgtctccaac gggtcggccg
     4561 gtcgccggcc gcacctcgtc atcgcggccg acgcgtacga cctgatcgag ctcgcgggcg
     4621 ccccactgcg gttcggcttc ccggacgtct tgaaccctca ggggcgctcg gtgatccgcc
     4681 ggatcgcgac ccggcacgtg cgagtatcag gcatggttcg gcacccgatc cggctctcgc
     4741 ggttcacccg gctgctgtcg gtggtcccgt gagcgccggc gccgaaccgg ctccgctgcc
     4801 ggcgccaccg ctccggcagc gccggtggat cccggtaatc gcgctgaccg ccgtgctggc
     4861 cgcggtgacg tccgggggct acctcacctc cgacgcgctc ggcgaggtcc gtggcggggc
     4921 cgtcgcagtc ggcacgtcgg tccgggtcac cccgttcccc ggctgggagc tcgccggccg
     4981 gtcgaccgat cccgaccgga tccgtctcac gagcggcagc accagtctcg atgtcgcagc
     5041 cttcccgttc gacgggaccg acgtcgacct gctcggccgg tacgtcgaca acatcctgga
     5101 gcccgacgcc gagcagttcc gagtctccga ggaggtcgaa cccgtccggc tcgcctccgg
     5161 gctcaccgga tcccggatcg cgtacgtggg cctgttcggc gacgtgcagg cgccgatcga
     5221 gggcgaggtg accgccgtgg tttcgcccac cgggcagggc gtgatcttcg acgggtgggc
     5281 acccgccggg cagctccagt tcgagatcga cgagatcgac gcgatgatcg aacgcgcgga
     5341 gatcgcctga tgagcgagcg gcgagggggc cggctcgcgt tcgagccgca gatcgtccgg
     5401 atgcctcgca actggggtcg ccagacgtcg ttgttccagt ggcggcagcc cgcgttctgg
     5461 ttgtacgcgg cgatcgtggt cgtcgccgcc gtctacacgg tctcgcagca acagctgttc
     5521 cagaagctat cgccctcggg gtgggcgctg tcctggggac tcctcctcct ctacgggctc
     5581 ccgctgttcc tcgccatcta cctgctggac ctgtacgagc gcgagccgat gtcgctcgtg
     5641 ctcggcgccc tcgtgtgggg gggcgtggcg gccacggtgc tcgcagggat cgcgaacgag
     5701 gggtggggcc tggtcgtcgc gcgtctcggg ggaccggtgt tcgcggccga gtggacggcc
     5761 gccctgacgg cgccgttcgt cgaggagacc gtgaaggtcg ccggcgtcgt gctgatctac
     5821 ctgatcgccc gtgacgagat ggacgacatg atggacggct tcgtctacgg cgcgatggtc
     5881 gggctcggct tcacgctggt cgaggacgtc ttctacgtca tcgcgatctt cggcggctcg
     5941 ccgggtgggg tgctcgccgg gttctacgtg cgcgtggtct cgagcggttt ctacgggcac
     6001 gtgctgtaca cggggctgtc cggcatgggg gtcgcgtact tcgtctcgag gcgaggcgag
     6061 gtcgcgttcg ggaggcggtt ggcggtcgcg ctcgggctct tcggctcggc ggttgcggcg
     6121 cacttcctgt ggaactcgcc gttcctgaac ttcttcccgt cgagcgtgga cagcgtcggc
     6181 gactggctcc ggatcccgtt cgcggcggcc gtcaagggcg cgccgctgct gctgttcgtg
     6241 gtcgtgatgg tccggctggc ccgacggcgt gagcgcatgt ggctcgagac ggccctgcgt
     6301 tcggaggtcg actcgccctg gctctccgag gtcgagctcc gggtgctcct cgacccgagc
     6361 gcgcggcgcc ggtcgcgacg cgagatgcgt gcccgcgccg gcgacgccgc cgcacgcctc
     6421 ctgaagcgcc tgcagcaaga gcagatcaac ctcgcgatgg tgcgggcgcg cgccgcctcc
     6481 gaagacggtc cggagctggt gcgacaacgg gccctctgcg cgtcgctccg cgacgcgttg
     6541 ctggcgatgc cgggcgccgc gctggccggt acccgacgcg agggttgatc ccggcgcctt
     6601 gcgcgagccg gaccctcgac gtagcgctcg cgcgcgtgac ccgtcacccg gtcctgcccc
     6661 acacgggggc ccgggtaggc ttcggccgtg agcctgaccg acgacgttct cgcgggcgac
     6721 ccccgagcgg tcgcccgggc gatctcgatg gtcgaggacg gcgccgccgg actggaggca
     6781 ctctcggcgg agctgttccc ccggaccgga cgagccttca cggtcgggct cacgggctcg
     6841 ccgggtgtcg ggaagtccac gatcgtgggc gaactggtcg gcgcggcgag ggcgcagggg
     6901 cgctcggtcg ccgtgttggc gatcgacccg acgtcgcccc tgtcgggcgg cgcgctgctc
     6961 ggtgatcgcc tgcggatgca ggcgcacgcg acggaccccg gcgtgttcat ccgctcgatg
     7021 gcgacgcgcg ggcacctcgg cgggatggcc ctcgccgcgc ccgaggcgat ccgggtcctg
     7081 gatgcggcgg ggcacgacct ggtgatcgtc gagaccgtcg gggtcgggca ggccgaggtc
     7141 gacgtcgcgg cggcgaccga caccacggtg gtcgtcgtct cgcccggctg gggcgacgcc
     7201 gtccaggtcg cgaaggccgg gatcttggag atcgcggacg tcttcgtcgt gaacaaggcc
     7261 gatcgcgagg gctccgaggt cgcggttcgc gacctccagc agatgctgcg gatgggtccc
     7321 gagctgccgt ggacgccgcc ggtggttccg accaccgcga ccgagggcac cggcatcgac
     7381 gacctgtggg ccgcgctcga gctgcaccgg gcccacctgg cgtcctcggg cgacctcgaa
     7441 cgggggcggc gcgatcggct gctgcgggag gtcgagagcc tctcgctgga gaagctccgc
     7501 ggccgggcgc gcgacctcct cacgaacgac ggttcgctcg ccaccgatct cacggaacgg
     7561 cgaacggacc cctaccggac cgcggctatc ctggaggagc ggatcaccac ccaaggttcg
     7621 tgatcgcgcg cgcgggccgg tacgcctgtg caacgagcaa acggcgcacc cggaggagga
     7681 ggatggtcga gcaacgcgaa cgacgatccg cagaggacac acagccgtcc ggggctcgga
     7741 ccctctcggg cgagccgctc gagcctctct acggccccgc cgacctcgcg gggttcgacc
     7801 ccgaggccaa gctcggacgt ccgggcgcct acccgttcac tcggggcgtc tacccctcga
     7861 tgtaccgcgg ccggttgtgg acgatgcggc aattcgctgg gttcggttcg gcccccgaga
     7921 ccaacgcgcg ctatcggttc ctgctcgaac gcggccaggg cgggctgtcg gtcgcgttcg
     7981 acatgccgac gctgatgggc cgggactccg acgacccgct cgccgagggc gaggtcggac
     8041 ggtgcggcgt ggcgaccgac tcgctcgatg acttcgaggc gctgttcgat tcgatcccgc
     8101 tcggcgacat ttcgacgtcg atgacgatca gcggccccgc cgtggtcgtc ttcgcgttct
     8161 tcctggccgc cgccgagcgc cagggcgtcc cgtgggagcg cctggacggc accctgcaga
     8221 ccgacatcct caaggagtac atcgcgcaga aggagtggat cttcccgccg aggccccacc
     8281 tccggctgat cggcgacctc atggagttct gcctcgaacg cgtcccgcgg tggcacccga
     8341 tcagcgtgtc cggctaccac atccgcgagg cgggggcgaa cgcctggcag gagctcgcgt
     8401 acacgctggc cgacggtttc gcgtacgtcg agctcggcct ggaccgcggg ctcgacgtcg
     8461 accggttcgc gccggggctg tccttcttct tcaatgcgca cgtcgacttc ttcgaggaga
     8521 tcgcgaagta ccgcgcggcg cgcaggatct gggcgcgttg gctgagggag cgctacggcg
     8581 cgaaggacga gtcctcgatg cggctccgat tccacacgca gacggcgggg gtctccctca
     8641 ccgcgcagca gcccatgaac aacgtcgtcc ggacggcgat cgaggccctg gccgccgtcc
     8701 tgggcgggac gcagtcgctc cacacgaacg cgctcgacga ggtgttggcg ctgccgaccg
     8761 aggaggccgc caagctcgcg cttcgcaccc agcagatcat cgcgcacgag accggcgtcc
     8821 cggacgtgat cgaccccctg ggcggctcgt acttcctcga gaccctcacc gaccggatgg
     8881 aggagctggc ggaggccgag ttcgcgcgga tcctcgcgat gggcgacggc tcgatgctcg
     8941 agggggtcct caccggcatc gagcgaggcg tcttccagca ggcgatcgcc gagtccgcgt
     9001 tcagtgagca ggagcggttc gagcggggcg acctggtcaa ggtgggggtg accgacttcg
     9061 tggaggcggg ggagcagccg atcgagatcc tcgagatctc cggcggcgtg gagacctcgc
     9121 aggtcgagcg ggttcgacgg gtccgcgagc ggcgggacgc ggcggccgcc cgagccgcgc
     9181 tcgaccgcct cggcgagctc gcggccacgg acgccaacct cgtcgagccg ctcgtggact
     9241 gtgcccgagc cctgtgcacc gagggcgaga tcgtggacgc cctgaggtcc gtgttcggct
     9301 cgtatacgga gacgcctcgg ttctgagacg ggcgtcgccg gcgcttgttg caagagcacg
     9361 cagttgccag aagatgcgtc agcgtggttt catggtgtct tccgattcgg ggagggatcg
     9421 cagggcgtgc tcgctgcctc ggaggccctt ggcgtcggcg tcatcgtgtc tgcgttcctg
     9481 ttcgggttcc ggcacggcat cgactgggac cacatcgccg cgatcacgga catcaccagc
     9541 tcgcaggagg accgtggccc ggcgctcgag tacggcacga tctacgcgct cggccatgcc
     9601 ctcgtcgtgt tcgcgatcgg ggtcctggcg atcgtccttg gggccaagct tcccgacggc
     9661 gtcgacgccg tgatggaacg cgtcgtcggc gtcacgctca tcctgctcgg cgtgtacgtg
     9721 ttctcggcgt tggtgcgtca cgggcgggag ttccggatgc ggagccggtg gatgctgatc
     9781 ttcagcggcg tccatggcgc gttccggaag gtccgcgggg ggcgccggcg cgaggcgcac
     9841 gatctcgagc cggtgcatcg acacgcggag caggatgggt ccctttcggt cgcggtcgcg
     9901 gaggatatcc ccgtgtccga gtggcatcac ggtcaccatg gccgtcccgg ccaccaccac
     9961 cacaagcacc ccgagccgac cgacgaggcc ttcatgaact acggccgggg gacggccttc
    10021 ggtgtcggga tgctccacgg catcggcgcc gaaacgccga cccaggtggt gatcttcctg
    10081 accgcggcgg gtgcgggcgg cgcactcgcc ggggtcctca tcctcgtcgt cttcctgatc
    10141 ggcctgctcg cgtcgaacac cctcatcacg ttcggttcgg cgttcggctt cgtgacggcc
    10201 tcgaagaact ggaccctcta cgtcacggtc gcggtcctga cggcgtcggc gagcctcatc
    10261 atcgggaccc tgttcctgct cgggaagggc gccgtgctgc ccgcgttgtt cggcgggtag
    10321 ggctcagccc gcggcgccgc tcgccttcct gcagtcggcg cacaggcctc cgatcgcgaa
    10381 gtgcgtgaga tcgggttcga acccgtggtg ttgctcgatc agccggatca gcgcgtcggc
    10441 ctcgccgatc gacaggtcgt cctcgtcccc gcagttcgag caggtgaggt ggacgtggcc
    10501 ggcctcttcg gtccggtgat actcggcccc gctctccagg tgggcgtggg cgagcacccc
    10561 catctcctcc aggagcgcga gcgtccggta gatcgttgac gcgttcacgc ccggcatgtc
    10621 cccctgaacc ttgcgggcca gggcggtggg ggagatgtga ccctgcgtgc gcatgatctc
    10681 ggcgacgatc gcccgccgct gcggggtcat ccgcagccca cgctcccgca gggccgagat
    10741 cacgtcgacg ttcgatgcct gttccgcggt ctgtcccacg tgggcgattg tagcagtgag
    10801 ttgcaggtgc aacggcaacc ctcggagcgg cctgagcggc gagggtctgc gaggataggc
    10861 cgtccatggg ggagttcgtc cgcatcgaca ccgagggcgg ggtcgccacg atccgcatcg
    10921 accgaccgcc cgcgaacgcg ctcgcgcgcc ccgtctcgct cgagctgtcc gaggccgccg
    10981 cgaccgtcgc cggcgacgag gccgttcgag ccgtggtcgt ctggggcggc gagcggatct
    11041 tcgccgcggg cgccgacatc aggaagatgg tcgggtacgg gccggacgag atcgcctcgg
    11101 acgtgggggc gctcgaacag gcctgccgcg acctcgaggc gatcccgaag gtcacgatcg
    11161 cggcgatcaa cgggttcgcg ctgggcgggg gcctcgaggt cgcgctctcc tgcgacgtcc
    11221 ggttcatcgc agaggatgcg cggctcggcc tgcccgagat caaactgggg atcatccccg
    11281 gctcgggtgg aacgcagcgg ctcccccgcc tggtcggcct cgcgaaggcc cgccacctga
    11341 tcctgacggg ccggcaggtg gatgccgcgg aggcgctcgc gatcgggctc gtcgaccggc
    11401 tggcggcgcc cgacgaggtc tatcgcgtcg ccgtcgacga ggcccgaacg ttcgccgccg
    11461 ggcccctgct cgcctacgcc gcggccaagc gggcgatggc ggccgccgat cacccgctcg
    11521 aggaggggct gcgggtcgag cgggaggcgt tcgtcgcgct gttcgccacc cgggaccagg
    11581 aggagggcat gcgcgccttc ctcgagaagc gcgaaccgaa gttcgagggg aggtagcgtg
    11641 cgcgttcgac aggccacggt ggaggacgtc ccgtccctgg tcgcgctgtt ccaggagctc
    11701 gatcggatgc aggcggactg gcgggtcttc accccgcgcc cgggcttcta cgacgaggtc
    11761 ggtgccaagt acaacgaggc cctcgccgac cccgacaccg tggtcctggt cgccgaggac
    11821 gaggacggcg aggtggtcgg gatggcgtac ggggaggccc gcaccccctc ccggttctcc
    11881 gacgagcgag ccctcgagct ctcgggcgtg gtcgtgcgcg ccggctaccg gggccggggt
    11941 gtgggccggg agctggtcca cgaggccgga cggttcgcgg ccgatcgggg ggtccggtgg
    12001 gtcgagttga agaccttcgc ccccaaccgg ggcgcgatgg agttctggga ggacctcggg
    12061 ttcaccgccc gggtggtgca gctcacgagc cccacgaagg ttctgctgga gcggctcgag
    12121 ggccgctgac gggcacgtcg ctcgccggaa aacagggtgc tcgggaacga accggcccgc
    12181 cgattggttc gaatgggatt gaagcctcgg ggcgaccggg gcgtcttcga accgaggcga
    12241 ggagacgatc gatgtccgac tcggcatacc gcggagcccg catccgcgag ctccctcggg
    12301 ccaaccgtac ctaccccgga ggtcgggtct gcgccgccga agggtgcagc acgaaacttt
    12361 ccatctacaa ccgatgggat ctgtgctggc agcacgagcc cgtccacacg tacatctctc
    12421 gcggcaagcg caggagccgc aaggagatgg aggccgcgta agccccgtac gcgccttccc
    12481 cagggaagcc cccgcccctg cgggggcttc cggcttgtcg actacgtctg gcctacgagg
    12541 agccgtcgcc ctcgggccga cccgaggcca gcacccgatc cgcgatcgcc tgcgcctcct
    12601 ccaggcggtt ccggtcgacg aacagccgga cgctccgctc ccagatctgg ctgaagaacc
    12661 ccgggttctt gtaccaccac acgatcccgg ccttctcgag ctcgccggcg atggcctcgg
    12721 cgtgctcgtg ggtgaaggcg cccaggtgga ccgtgtggac gtcctcgctc acggcacgag
    12781 caccgcgggt cgccacccgc ggaccgagct cacgagggcg agctcccccg cgccgcggag
    12841 gccgtccacc gtaacgggtc gctcgatcag ccgcccctcc cgcagcagca cggtccggta
    12901 ggtgcccggg aggagccccg catcgatcgg cggcgtcacc cagactccct ccaggcggac
    12961 cgcgaggttc gcgaccgtcg actcggtcac ctcgccgcga tcgttgacga gcaccacgtc
    13021 gttcacatcg gggcgtcgat cgcggcgccg ctcgtagggc ccgcggcggg tcgtcttgtg
    13081 gaagagccac acgtccgacg gatccacggg ctcgtcgtcg agcgacacgc ggacccgctc
    13141 ctccgcgggc ggcaggtccc gaacgtcggt cgacaacgcg ccgtcgcgcg cgagcgtcag
    13201 ccggacgacg ctcggctcgg tgacatcgtc gacggcctgc ttgagcgttg cggccgcctc
    13261 ctcgagctcg aaccggaagc cgaagtaccg ggcggaggcg gcgagccgct cgaggtgctc
    13321 gttcaggtgc cggaacccct cggccggcac gtgcgcgagg ctctcgaaca gctcgaacgc
    13381 cggtcgcacg tcgctcaaga cacgtgcctt cgcgaggatc tcctcgtatt cgcccgaggc
    13441 cgacgagtcg tgggtgatgc cgcccccgac accgaactcg gccgtccggg tctgggtgtc
    13501 gaggacgacg gtgcggatcg cgacgttgaa gttcgcgcgc ggctcgcccg acccgggcgg
    13561 cgacaggtac ccgatcgcgc cgcagtacgg ccctcgggcc gagtcctcga ggtcggcgat
    13621 gatcctggtc gtgcggacct tcggcgcgcc ggtgaccgat ccgctcggga acagggcccg
    13681 gaacgcgtcg agcagcgtga tctcgggtcg caggtcgcct tcgatcgtgg aggtcagctg
    13741 ccacacggtc tcgaagcgct cggcctccac catccgttcg acctcgaccg agcccgttcg
    13801 gcagatgcgg cccagatcgt tgcggagcag gtcgacgatc atcgcgttct cggcgcgctc
    13861 cttcgacgac gcgaccaggc gcgccgcgat ctcctcgtcc tcggccggcc agcgacctcg
    13921 cggcgcggtc cccttcatcg gtttcgtggt gatccgctgg ccgtcgatcc ggaagaacag
    13981 ctccggcgac gccgagagaa cgcggaagcg cccaacgtcc aggaacgcgg cgtacccgcc
    14041 ccgctgggcg aggcagaggt cccggtagaa ccctcgctcg tccccctgga tcatggcccg
    14101 gagccggatc gtgtggttca cctggtaggt gtgtccggcc gcgatcagct cgcggatgcg
    14161 ttcgacggcc gcgtcgtaga tcgcgcgatc gacgctcggg cgccagggcg aaccctccga
    14221 tccggacgga tcgagccgtc ccggttcgag cggcggcagg tcctccttgc gttcgaacag
    14281 ggcgaaccag gcgagcggga gctcggcgaa gggatcgtcg ggcgcccgga cgtgcaccga
    14341 gagctcggga tcgaggcctg gcgccgcctc gtacgcgacg aaccccgccg cccacagccc
    14401 cctcgcggcc gccgaccccg ccgcgtcgat cgcgccggcg acctcgccgg gccggcgggc
    14461 ctcgatgacg ccgaccggtt cggcgagccg gaacgcgcgc tcggcgccgg ccaccaggtc
    14521 gtcgaaacgc gcctcgatca cgtcaggccc ccgtcagccc cggcgagctc ggatctcctc
    14581 gatcaccttc gggacgaccg tgagcgcgtc gccgacgacg ccgaggtcgg cgagccggaa
    14641 gatcggcgcg tccccgtcct tgttgatcgc gatgatccgc ttggcgccct tcatccccac
    14701 gacgtgctgc gtcgccccgc tgatgccgac ggcgatgtac acctcgggct tgacggtctt
    14761 gcccgtctga ccgacctggt acgagtaggg cacccatccg gcgtcgacca ccgcccggct
    14821 cgccccgacg gccgcgtcgc cgatcgcggc ggcgaggtcg tcgaggagct tgaagttcga
    14881 cgggtcctgc agcccccggc cgccggcgat gacgaccttc gcgtcctcca gccggggacc
    14941 ggcggcggtc tcttcgtgac gttcgatgcg tcgtaccgtc gtgaattcgt cggggaccgg
    15001 cgtgtcgact ggcacgactt cggcggtgcc gccgttcggc tcgggcgcga aagacttcgg
    15061 tcgcaccagc acgagtcgtg gcgtgggccc gtcgagcgcg acgtccacga cacggctccc
    15121 gccgaagatc tgcgtctgcg ccgacccggc gcccgtcacg tcggtcacgt tgctcatcag
    15181 cgtcgatccg agcctggcct ggagccggcc cgcgacgtca cgcgcgtcgt agctcgtcga
    15241 gaacaggacg agatcgggcg tgtgctcctg gatcagcgcg tggagcgtgt gcgccgcggg
    15301 cctcccgagc gtgtccgcgt agacctgctc gtcgctcgcg aagaccctgg cggcgccgta
    15361 ctcgccgagc gtgatcgccg cggccctcgc ccccggaccg agcgcgacag cggcgacctc
    15421 gtcgccgatc gtccgagcct tggtcagcag ctcgagcgcg gacgggctcg ggccctcggg
    15481 cgtgatatcg gcgtagaccc agaccgtcgt cacgtcagat cgccttcgca tcggcgagca
    15541 gctcggcgat cttggaggcg gcgtcgtcgc cggcctggat cacgaccccg gccgacttct
    15601 ccggcgcctc ggagaccgag gtcacacgct gcgaggcggc cacgtccgcg tcgaacaggc
    15661 cgaggtcggc gagcgagagc tgctcgagcg gcttggattt cgcggccatg atgcccttga
    15721 gggtggggta ccgcggttcg gcggcgctcc cggtcaccgt cacgagcgcg ggcagcgggc
    15781 tcgtcacgac gtcgtacccg agctccgtct gccgctccat gcggagcgcg ccctcctcga
    15841 cctcgacctt gcgcgcgaac gtcgcgctcg gaatgccgag gagctcggcc acggtcatcg
    15901 ggagcgtgcc ggtggagccg tcggtggatt cgacgcccgc aaggaccagg tcgaacggtg
    15961 cacgcaccac ggcggccgcg aggaccctcg ccgtggcgag cgcgtcggcc ccgcggagcg
    16021 cgggatcggt cacgagcacc ccgccacccg cgcccatcga gaaccccctg cggagcgcgc
    16081 ccgcggcgac ctcgggcccc atcgagacca gcgtcacctc gccgccgtgc atctcgacga
    16141 gccgcagcgc ggcctcgacc gcgaactcgt cgcccgggtc cagggcgccg tcgacgccct
    16201 cgcggacgag caggaggtct tccccgagcg tgggcgtgcc ctgcggttcg gggacgtact
    16261 tgacgagcac gacgacgtgc acgggatcct cgactcctcg ttgggccgct ggctgggccg
    16321 aggatacccg cgacgacgcg ggcgcacgca cctcgtagca tcttgaccat gtcgaagggg
    16381 accgtgctcg tcgcgatgtc cggtggcgtg gactcgtccg tcgccgcctg cctgttgctc
    16441 gagcagggct acgaggtggt cggctcgcac atgcggctgg tccacatgga cggggtggag
    16501 cacggctgct gcggccccac ggctcgcaac gatgccgcgg aggtcgcgcg gatcgccggg
    16561 ttcccgttcg agatctgcga tctctcggac gagttcgagc ggaccgtgat cggggatttc
    16621 gttgccgagc acgaggccgg ccgcacgccg aacccgtgcg cgcgctgcaa cggcgagatc
    16681 aagttcggcg cgttcctgcg ccgcgccgac gagctcggga tcgacgcggt ggcgacgggt
    16741 cactacgtgc ggacggatcg gatcggcggc cgtctccggc ttctacgcgg cgccgaccgc
    16801 gcgaaggacc agtcgtacat gctgcacatg ctcggtcagc gcgagctgtc gagatcgcgg
    16861 ttcccggtcg gcgggttgcc gaaggccgag acgcgcgcgg ccgccgaacg ctacgggctc
    16921 cccgtcgcga gcaagcccga ctcgcaggag ctgtgcttcg cgccgagcgg ccaggcgggg
    16981 gcgtacctgg tctcggtggc cccgcacctg atgaacgaag gcgaggtcgt cgacaccgag
    17041 gggcgcgtgc tcgcgcctca cgcggggtcg gcaaccttca ccatcgggca gcggcgcggc
    17101 ctcggggtct cgggtcccga gccctcctac gtgctcgaga tcgacgcccg ggccaaccgg
    17161 gtggtcgtcg gaccggggga gctgctcgca cgctcggggg cgatcgccga tcgggtgtcg
    17221 tgggtggccg gcgagctccc ctcccgcgtg cccttcgagg cggaggttcg gatccgctac
    17281 cggggagacg acgttccggc ggtggtcgag ccgctcgggg acaccgtccg gatcgagttc
    17341 cggacgccac agcgcggcgt cgcgcccggt cagagcgccg tcgtgtatcg ggacgaggag
    17401 ctcctcggcg gcggccggat cgtcgacgcc ctccgctgac gaaccggcag ttccccgcga
    17461 ggcgccggat ggccgtgggg tcggcccgcc cggatatcat cgccgcgtgg agggccggcg
    17521 cccgtgaggc tgctcaagcg caagtcccgc accttcgaga tccaggtgca ggacatgacg
    17581 ctgcacatcc aggccgcgac cgacgtcgac gaggagtcac gcgcggccgc gctctcgttc
    17641 tgggagctcc tgcacaccta ctcgatccgc aacccggagt tccgctcgtc caagcggccc
    17701 gtgaaggtgc ccggtgacgc ccccgagatc gtgcgcgaga tcgcctcgac ggccgcgcgt
    17761 gcgggcgtcg gccccatgtt ctcgcttcag ggcgccgtca ccgatcacgt cggccggttc
    17821 ctcgcgcacg aagtgaggga ggtgaccgtc aactgcggcg gcgactactt catccgttcg
    17881 cggcgccggc agaagctcac ggtcctgcgc cggccgggtt cctcggtggc cgtcgtggtc
    17941 ccccccaacc gggagggcct cggggtctcg atgacgctgg ggcacgggcc gaggcgctcg
    18001 gaggtggacg ggctggccgt gttggccgag tcctgcatgc tcgcgggcgc cgcggccgcg
    18061 ggcgtccagg cgatcctgac caaggaggac ggactgcacg gggccttgac gtacctgaag
    18121 caggtgcccg gggtgatcgg cggggtcgtg ttcctcggcg agcggatcgg catggctggc
    18181 gggctggagc tcgcggcttg acgcgggtcc ggacgtgagc accacgagcg aggaacggga
    18241 gctcgcgaac gcgaagctcc gcgtcgagga gctgcgcgag caggcgaact tccactcgta
    18301 ccgctaccac gtccttgacg accccgaggt ctccgacgcc gagtacgacg acctcatgaa
    18361 ggagctccgg gcgttcgagg agcgcttccc cgagctggtg acgcccgact cgccgacgca
    18421 gcgggtggga gcgacgcccg ccgacctgtt cgcgcccgtc gaacaccgaa ctccgatgct
    18481 ctcgctggac aacgcgttca gccgcgagga gctcgacgcc tgggccaccc gggtcgaacg
    18541 cggggtgggc tcgggcgccc agtactcgtg cgagctcaag atcgacggag tcgcgtgcgc
    18601 cctcacctac gagaacggcg cgctgaccaa gggcgcgacg cgcggcgacg ggcggaccgg
    18661 cgaggacatc acggccaacg tccggaccgt tcggagcgtc ccgcagcgcc tggacgtgaa
    18721 ggtgccgccc gcctacctcg aggtccgcgg cgagatctac ctgccggtca gggcgttcga
    18781 gcgcctgaac gaggagctgc tggaggccga gaagcggccg ttcgcgaacc cgcgcaacgc
    18841 ggccgccggc tcgctccgcc agaaggaccc gaaggtgagc gcgtcccgac cactcgctct
    18901 gctggtgcac tcgttcgggt tcgcggacgg gatccgcttc gactcgcact cggggttcct
    18961 ggactggagc cgcgaggccc ggctccccgt cgcctccacg tccgaggtcg tgaaggacgt
    19021 cgacgacgtg gtggcgtacc tccagcgctg ggaggccgac cgccacagcg tgccgtggga
    19081 gatcgacggc gtggtggtca aggtcgatca gaccgcgctg cagcaggagc tcggcgcgac
    19141 cagccacgcg ccgcggtggg cgatcgccta caagttcccg cccgaggaac gcacgacgct
    19201 gctgcgcgag atcggcgtgc acaccggcag gacgggcatc gtgaccccgt tcgcgttcct
    19261 cgagcccgtg caggtcgccg gcgtgacgat ctcgaccgcg acgctgcaca acgaggacga
    19321 ggtccggcga aaggacgtgc gtgagaagga cacggtgatc gttcggcgcg cgggggacgt
    19381 gatccccgag gtcgtcgggc ccgtgctgtc caagcgcagg aagggcacgc ggcgctggac
    19441 gttccccacc acgtgcccgt cgtgcggcac cgtgttggtc cgcaaggagg gcgaggccta
    19501 ccggcgctgc ccgaacaagc gcgagtgccc ctcgcagaac atcgagtggc tgttcagctt
    19561 cgcctcgcgg ggtgggctgg acatcgaagg cctcggctac aagaccggga tcgcgctgat
    19621 cgacctcggg ctggtgaccg accccggcga cgtgttctcg ctcaccgcgg acgacctctc
    19681 gcagctgccg gggttcgccg acaagtcgat ctccaacctc atgtcgtcga tcgaggccgg
    19741 caaggaccgc ccgatctggc ggctcctcac agcgttgaac atcccgcacg tcggcaccca
    19801 cgtcgcgcag gtgttgtcga acgcgttcgg gtcgatcgac gcgcttctga aggcctccga
    19861 gcaggacatc gacgacgtcg aggagatcgg acccgagatc gcccggagcg tccacacgtg
    19921 gttcgccgac aaaggcaacc gtaggctgct ggagaagctg cgggcggccg gtgtccggat
    19981 gaaggacgtg gccccgagga aaccaaaggg gaaacggccg ctcgccggca agacgatcgt
    20041 cctcaccggc ggcctccagg ggctgtcgcg ggacgaggcc gcggccgccg ccgaggccgc
    20101 cggcgcccgc gtggtctcga gcgtgtcgaa gaagaccgac ttcgtcgtcg tcggcgaaaa
    20161 cccggggacg aaggcagcca gggccgagga gctcggcgtc gagacgatcg acgagcggga
    20221 gttcgcgaag cgcctgggcc gcaagcaggc ctgaagcgcg cgctcgagca cctgtggcac
    20281 gattgcccgc gtccccgacc ggaaggagcc cgcgtgacgg ccgtcgtgat cgacgggaac
    20341 gcgatcgccg agcagctccg gcagagcatc gccgacgagc tccaggtgct gcgcctgtcc
    20401 gaggttcgcc ccgggctcgc gacggtgctg atcggggagt cgttcgacgc gcaggcgtac
    20461 gagaggcacg tcaagcggtt ggccgagagc ctcgactacc ggtacgtgtg cgagagtcta
    20521 tccgcggacg tcgagctcgc cgacgtcatc gcgaccctcg gcaagctgaa cgccgacccg
    20581 cggatcaccg gcatcctcgt gctgcaccca ctgccgccgc acatccccga ggccgagatg
    20641 aacagcgccg tcgacccgcg caaggacatc gaggccgtgc atccgatgaa cgccgggctg
    20701 ctggcgcagg gccgcccccg cttcctgccg tccaccccgg cctcgtgctt ctacctgctc
    20761 gaccactacg cggacgcgca cggcggcggg cacgagagct tctactcggg ccggacgctg
    20821 gtggtggtcg ggcgttcgaa cagcgtgggc aagcccgcac agatgctcgg tctccagcga
    20881 gacgccaccg tgatcgcgtg ccacagccgg accaagccgc tcgaggcgta cacgcggcag
    20941 gcggacatcc tgatcgtggc cgccggcgtc cgcgggatcg tcgacggctc gatggtccag
    21001 gagggcgtgg tggcggtcga cgtcggcatc cacgcggtca aggacgagga gaccggcaag
    21061 accaggatgg tgggggacct gaacttcgag agcgtggccg agaaggccga ggcgatcacc
    21121 ccggtccccg gcggggtcgg gccgatcacc gacgtgtggt tggtcggcaa cgcgctgatg
    21181 gcggcggcga tcgctcatca ggtggagccg cggttcggcc tgttccgctg agtgtgtgtc
    21241 ggattcggtg tggttcgggc gatcgcctga gtctggacac ccagtgagat cccgcgctcc
    21301 gacagaacct gacagacaga accctgacag acagaaccac ggacccctcc gatcgcgatc
    21361 tcgagatcac ccgactccga gcctccgcta gactgcgcgc atgccggtcg agatcgacat
    21421 cgagcacgtc gcgcgtctcg cgcgcctcga gctcacgcag gacgagaagg ctcgactgcg
    21481 cgagcagctc ggggtgatcc tcgaggcagc cgcgaaggtg agcgaggtcg ccaccgacga
    21541 cgtgccgccc acggcgtacg cgatcccgag gtcgaacgtc ctccgacccg acgagatcac
    21601 gccgtcgctc acggtcgagg aggtcctgtc caacgcgccc gaggtcgagg acgaccggtt
    21661 caaggtcccg aaggtcgtgg aggtcgagtg acagagctgt gcgaccgaac ggcgagcgac
    21721 ctcgcttccg cgcttcgcgc gggggagtgc tcggccgccg agatcctcga gtcgagcacc
    21781 gaacgcatcg acgacgtcga cgggcgcgtc ggcgcgttcc tggcgcggac cgacgagctc
    21841 gcgcgcgagc aggcgcgcac cgcggacgaa cgcatcgccg ccggagatgc cccgccggcc
    21901 gcggggatcc cgctcgcgct gaaggacgtg ctgtccacgc gcggggtcac gaccacatgc
    21961 ggctcgagga tcctcgagac ctatgtgccg ccgttcgact gcacgccgtg ggagcgcctc
    22021 gaacgcgacg gcggcgtgct ggtcggcaag accaactgcg acgagttcgc gatggggtcg
    22081 tcgaacgaga actcggcgtt cggaccggtc cacaacccgt gggacctcga cctggtgccg
    22141 ggcggctcga gcggcggcag cgcggccgcg gtcgctgcgg gggaggcggt gtgggcgctc
    22201 gggacggaca cggggggctc cgtgcgtcag ccggcgtcgc tctcgggcgt ggtggggctc
    22261 aagccgacct acggcctgat cagccggtac ggcctgatcg cgttcgcgtc ctcgctcgat
    22321 acggttggga cgctcacgcg gagcgtccgg gacgccgccc tcctgctctc ggcgatcggg
    22381 ggaaaggatc cgatggacgc gaccagcctg gacgagccgc ggcgcgacta caccgagggt
    22441 ctcgacggcg agatctcggg catccgtgtc ggcgtcgtca aggaggcgtt cggcgagggc
    22501 gtggagccgg gcgtccggga cagcgtccgg cacgcggtcg acgagctcgc gaacctcggg
    22561 gcgcgggtgg gggaggcgtc gtttccgcac gccgactacg cgctctcggc ctactacctg
    22621 atcgcaccgt cggaggcgtc gtcgaacctc gcgcgctacg acggtgtccg ctacggactg
    22681 cgcgtcgacg gcgcggactc ggtcgagatg atgacgagga cgcgcggggc agggttcggc
    22741 gccgaggtca agcggcggat catgctcggc acatacgcgt tgtcggccgg ctactacgag
    22801 gcgtactacg gccaggcgca gaaggtcagg acgctgatca tccgcgacta cgaggcggcg
    22861 ttcggggcgt tcgacgtgct ggtctcgccg acgtcgccga cgaccgcctt cccgatcgga
    22921 gcgaagatcg acgacccgct cgcgatgtac ctgaacgatg tgttcacgat cccggcgaac
    22981 ctgtccggca tgccggccat ctcgatcccg agcggcgtcg acggggccgg cctgccggtc
    23041 ggcctccagt tcacggcgcc cgtcctggcc gaacccaccc tgctacgggt cgcgaacgcc
    23101 ctcgagggcg cgctcgggtt cgacgcccgc ccgccgctgc tcgcgggatc gcccgcgtag
    23161 ggcttccgga gatccacgcg gtgtgggtgg tgctcgctac gttccttcgt ggtcggtggc
    23221 gacaacgagc cgtcccccgc tgacgatgcc ctcttcggca tcgatccccg ccgagaggtc
    23281 tccgctcggt tgcgatgcct cccgcggcat cgggggtccg ccgggaacct actggccagc
    23341 ctccatgcca cgaaaggcat cgccgaatcg aaggttcctc gctgggggcc ggctacctcg
    23401 tcctggtcgc gcgggagctg accggactgt cacaacgcag gctcgaccag cgcgtcggga
    23461 cctcccagcc ctcgttggca aggatcgagt cgggatcgcg gatccggctt aggccgcggc
    23521 ctcctccagt tcctccgccg gtcgttcggg ctcgccccga gcgctcccgg ccgacacgat
    23581 cagcgccacc gccgccacga tgaggacgcc ggcgagcagc gtgctcgccg tgaacgtctc
    23641 gtccaggatc aacgcgccca cgatgacggc gccgatcggc gtcacgtacg cgtaggtcgc
    23701 gacgagcgaa gtccgggcgt tgcggagcag ccacaggtag cacgtgaagc cgatccacga
    23761 accgaccacc accaggtagc cgagcgcgat ccacgatgcc cgtgaggtcg atcccggctc
    23821 gagatccccg aactcgccga cgagcccgcc gacgacgaag aacaacccgg ccgcgacaag
    23881 catctccatc cccgcggcca cgagcgggcg gcgcggcagc gcggcgtgac gctggtagat
    23941 cgatcctgcc gcccagctgg ccgccgcgcc gagcgcgagc acgagcccgc ccatgtcgat
    24001 ctcgccctcg agcgcgttcc cgccgacgag cagcgcggcc ccgatgaacc ccaccgcgag
    24061 cccgatcacg acgacgcgcg gctggcggtg cccgaggacg acgcggtcga tcgtcgccat
    24121 ccacagcggg accgtggcga tcgccaggcc ggccattccc gaggggatcg tgcgttcggc
    24181 ccagacgatg ccgccgttgc cgccgaacat catcagcgcc gccaccacgg ccgcaccccg
    24241 ccattgggtt gcgcgcggac ggtcgccgcg gcgatcgccg aaccggagcg cgatggggag
    24301 cacgaggccg cccgcgatca ggaagcggag gccgcccccg agcaacggcg gcatcgtctc
    24361 gttgacgacc cggatggcga cgaacgtcgt gctccacgcc acgtagacga gggagagcgc
    24421 ggtccagatc gcggcggagg agccgttccc tcgagcggtc ggttgctcga tcggagcgga
    24481 catgcggacc acgatacggg ccgcccgccg ttggtgtcac tatcgttcgg acgtttggcg
    24541 gttacgggac gacgagcgga acgaacgcgt tgcttgatcg ctcgggcgct ccgcgaccgg
    24601 acgcgacgga cggctccgat aggcttggcg cgtcatggcg gacgcgttcg aaaccgtgat
    24661 cgggctcgag tgccacatcg agctgtcgac ggactcgaag atgttctgcg ggtgccgaaa
    24721 cgcgttcggc gcccctccga acacgaacgt ctgcccggta tgcctcggcc tgccggggtc
    24781 gctccccgtt cccaacgaga aggcgatcga gtcgatcgtg cggatcgggc tggcgctcgg
    24841 atcgacgatc gcgccggact cgttgttcca ccggaagaac tacttctatc cagatatgcc
    24901 gaaggactac cagatcagcc agtacgacct gccggtgtgc gtcggcgggc gcctcgagat
    24961 cgaggtcgac ggcgagcgac ggacgatcgg cgtgacgcgc gtgcacatgg aggaggacac
    25021 gggcaagacg acgcacgtgg gcgccggcgg gcggatcggg caggccgagt acgccctcgt
    25081 cgactacaac cgcgccggcg tgccgctcgt cgagtgcgtc tccgagcccg acctgcgctc
    25141 ggcggaggag gcgcgcgcct acttcgtcgg gctccgcgcg accctcgagg cgctcgacgt
    25201 ctcggacgtg cggatggagg agggctcgct ccgctgcgac gcgaacatct ccacccggcg
    25261 cacgggggag accgagctcg gcaccaaggt cgagatcaag aacctcaact cggtgcgctc
    25321 gctcgaacgc gcgctgcggt acgaggagga gcggcaacga accgcgctcg aacgcggcga
    25381 gccgctcgtc caggagaccc gccacttcga cgaggagacg ggcgcgacgc acacgctgcg
    25441 ttcgaaggag gaggcgttcg actaccggta cttccccgag ccggacctga cgccgctcga
    25501 acccgaccgc gcgtgggtga aggtgctgga ggcagagctg cccgagctcc cggccgcgcg
    25561 gcgcgagcgg tacgtggacg ggctcggtct gagacccgag caggcccgga tcctcgcggg
    25621 ctcgccctcg gcggcgggct tcttcgaggc gacgatcgcg ctcggggccg atccggcatc
    25681 agccgcgaac tggatcaccc aggacctcgc gggactcgtc aacacggcga ggatcgagct
    25741 cgccgacgcc agggtgacgc ctcggcacgt ggccgacctc gtcgcgctcg tcgccgacga
    25801 gacgatctcg gcgaccgggg cgaagcaggc gctcgaggag gcgttcgaaa ccggcgacgc
    25861 ggccgaggcg atcgtggagc gccgcggcct gcgccaggtg gtcgacgcct cggcgctcga
    25921 ggcctgggtc gacgaggcga tcgccgagaa ccccggtccc gtcgagcagt tccgcggtgg
    25981 caaggagggg gcgctcaacg ccgtcctcgg gcaggtgatg aagaagtccg gtggctcggc
    26041 cagtcccaag gccgcccgcg aactgctcct ccagcgcctg actggatcct gatccggcag
    26101 ccggtgaggg tcgaccggag ggcgccgaaa cgctacccct ggtggcagct gatcctgctc
    26161 gggctggtcg cgatggtcga ggcgctgttc cggccgctcg cgcgcatgtg gcgctcggag
    26221 gacccgctcg acacctacgg gctcgcgcac ttgggcagcg tggcgggcga cgcgatggtg
    26281 gcgatcgccc tcgcggggtc ggtgttcttc gagatcccgc cgcccgaggc cgaggtgaag
    26341 gtggcggtgt tcctcgcgct cacgatcgcg ccgctcgcgg tggccgggcc gttcctggtg
    26401 ccgctgctcg accgcgcggg gccccgacgg gcgatctcgt tcggggcggc cgccgcccgg
    26461 gccgtggtgt gcatctatgc cgcgccccgg ttcggcacct tgctcctgtt cccctgcgcg
    26521 ttcctgctcc tggtgctctc gaaggtgcac gcgatcacga agaacgggct cgcggtcgcg
    26581 tacgcggggc cgaacgaggg cctcgtccgc gcgaacgccc ggctcgggcg gatcgccacc
    26641 ggcggtgcgg tgctcgcggc cgggccggcg ttcgcgctcc tcaagctcgg ggccgccgag
    26701 gccgtcctgt acggcgccgc cctcgtctac gggatcgccg cagcgctgaa cctgcgcctg
    26761 ccccacccga gggtgcgcgg cgggcaggcc gcggtcagca agctcggctc gatcccccag
    26821 ctcaccgcgc cggcgatcgg cgcggctggg ctgcgcgccg cgagcgggtt cctgctgttc
    26881 gcgctggcgt tcgcgctccg gcgtagcggc gagcccacct ggtggttcgc cgtcctcgcg
    26941 ggcgcggcca cggcgggggc gtacctggga gacctgatcg tgccccggct cccggcgaac
    27001 ctgcgcgagg aggccgtcgt gatcggctgt ctgatgggcg ccggggtggg cgcgacgctc
    27061 tcgttcttcg ccttcgagct cgtggtgctc gtgctgttcg ccatcgtcgt gggcgcgtcc
    27121 tccgagctcg ggcggctcgc gttccagacg ctgatgcagc gcctcgcgcc gggtggttcc
    27181 tacgggaggg tgttcgtccg gtacgagatc ctgttccagc tcgcgtgggt cggcggcgcg
    27241 ttgatcccgg cgctcgcgcc gatcgacttc cgagacggct tcctgatcct cgcggcgttg
    27301 tacctggtcg cgatcatcgg gtacctgacc ccggacatcc tcgcgcgccg gcgtggatga
    27361 gttcgctgat gggtagggta ggctgcgccg gagcccttcc gggcgtgacg ccgcgccgga
    27421 agcgaagatc gtggacgaag ggggagcccg agcatggcgg agtgggtgag ggtcgggtcg
    27481 gcgggctcgg tgcccgaggg cgagatcacg gcgttcgccg caggcgagcg ccaggtggcc
    27541 gtcgcgaacg tcgaggggga cctgcacgcg ttcgacgaca cgtgcaccca tcagcagtgc
    27601 tcgctcgccg agggcgagct ggacggtacg acggtcgagt gcgcctgcca cgggagcctg
    27661 ttcgatgtca tcaccggtga ggcgatcggc gggcccgccg tcgaccccgt cggcgtgttc
    27721 gaggtcaagg aagaggacgg cgagctgaag gtcctggtcg acgaggagtg accgcgttcg
    27781 tcgtcgtcgg ggctggtgtc gcgggcgcga cggccgccgc caaggtccgt gaggaggggt
    27841 tcgacggcga cctcgtcctg atcggcgccg agccgatgcc gccctacgag cggccgccgc
    27901 tctcgaagga atacctgcgc ggcgagtccg accaccactt cgtccggccg cccgagtggt
    27961 acgcggagca ccagatcgac gcgcgcttcg gcgcgaaggc cgagcgggtg aacgtcagcg
    28021 accgagtcgt cgagctcgag ggcggggagc gcgtgccgtt cgaccaggtt gtgatcgcca
    28081 ccggcgcccg caaccgcagg ccgaagatcg acggcctcga tctgcccggg gtgttcgacc
    28141 tccgtttcgc gcctgacgcc gacgcgatca agcgcgcggc gaaggaaggc tcgaaggccg
    28201 tcatggtcgg gatgggcttc atcggcgccg aggtggccgc ctcgctccgg cagctcggcc
    28261 tggaggtcac ggtcgtggag ttcttcggca cgcccctgga gcgggtgctc gggccgacgc
    28321 tgggcggcgc gatcgagtcc ctgcaccgcg accacggcgt cgagatgatc ttccgcgacg
    28381 gagccgagcg gttcgagggc gacgggcggt tcgaggcgat gatcacgaag gaggggcgcc
    28441 ggatcgaggg ggacttcgcc gtcgtcggtg tcggcgtcga gcccgtcacc gaggtcgccg
    28501 acgggggtgt cccggtcgac aacggcatcc ccgtcgacgc gaacctgagg acgagcgtcg
    28561 acggggtctg ggcgatcggc gacgtcgccc ggcacgacca ccccgtgttc gggccgatcc
    28621 gcgtcgagca cttcgacaac gcgttgaaga tgggggagca cgtcgcgaag aacctgctgg
    28681 gcacgccggc ggcgttcgac gacgcgcact ggttctggtc ggatcagtac gacgccgaga
    28741 tccagatggc cggtttcgcg accgagtggg acgatatggt cgtgcgtggc agcatcgagg
    28801 accggagctt ctcggcgttc ctgctgaagg acggccggct cctgtcgacg ttcagcatga
    28861 accggaagtt cgacgtccgc cgttcgatgc cgctgatcgc cgcgaaggcg caccccgaca
    28921 gccggcagct cgccgatccc gagttcgacc tccgtcagct tcacccgccg aaggagcagg
    28981 cgagatgatg cagccgaaca tcacgcacat gccctgcgac ctgaaccagc agcagaagcg
    29041 gcacgacgct cggaaggatt cacttgctcg agcggtggcc tggcggatgg gcaagcgttc
    29101 gtggctcgac cggctgctcc gccggggaac gccggggctc gagctctcgg ccgaggacct
    29161 ccggccccgc gagaccgaag acgcgttcca cggtttccgg gtctaggcct tcctcggcgt
    29221 ctgccccggt gcccgcggcc gaggatcaac gcgatcgcat gagcgcgcgg atcggcggcc
    29281 agagcacgcg gtacagcccg taggcgagga gcgcgagccc gagggcgacg cggatctgct
    29341 cctcggtgaa ccgggtccgc ttggcgaccg gcttggcgac cgcgttcccg acggcgcccc
    29401 gccagccggt gagcgtcgat tcgccgagcg cggcgaccgt tccctgtcga tccgtcatcg
    29461 tgcgaggtct tcccacgatg gggcctggtg aaacgctcag tcgacggtga ggcgggccga
    29521 gatctcctcg ggcctgccct cggcctcgac cttcccgtcg cggaacagga tcacgcggct
    29581 cgagtagcgg agcacctcgg ggttgtgcgt cgcggcgagc accgcgccgc cgcgccggat
    29641 cacctcccac agggcctcga ggatcaggtc ggcgttctcg gtgtcgacct ccgcgatcgg
    29701 ctcgtcggcg aggaccacct cgggctcacc gatgagagcc cgcgcgaccg cgacccgctg
    29761 ttgctgcccc gcggacagct cgaacgtccg gttgtacatc cggtcgccga gcccgagccg
    29821 cgccagccac atctcggcgc gagccttgga ttccttgagg tcggtcccgg agacgcggag
    29881 gggcagctcg acgttctcga ccgccgagag cgtcggcagc agcccgaagc tctggaacac
    29941 gaagcccatc ccgctccgac ggagcgcgat ctgctcgtcg aacgagagct tgcggatcgg
    30001 ccggtccttc caccggacct cgcccgcgtc cggcgcgtcc aggcccgaca gcaccccgag
    30061 gaacgtcgtc ttgcccgagc ccgacggccc catcagcgcc acgaagtcct tcggggccag
    30121 cgagat
//
LOCUS       DATBRK010000289         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2212330, whole genome shotgun sequence.
ACCESSION   DATBRK010000289 DATBRK010000000
VERSION     DATBRK010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212330"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1647
                     /gene="fusA"
                     /locus_tag="VIH70_08790"
     CDS             <1..>1647
                     /gene="fusA"
                     /locus_tag="VIH70_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8116497.1"
                     /translation="VERSLRVLDGAIAVFDAVAGVEPQTETVWRQADRYSVPRIGFVN
                     KMDRTGADFGRTVEMMVERLQANPLVLQLPWGSEGDFQGVIDLVEMKALHWVSEMGEE
                     WETTEIPAELADAAKRAHHDLFEKLADHDEWLMEKFVHEEEPTADELRRAIRRATLSG
                     EGVPVLLGSAFKNKAIQPLLDAIVAYLPAPSDVPPVEGHTLKGEHAERKPEDSEPFSG
                     LVFKIMSDPFVGRLTYVRLYSGILRAGSHVENTTKERKERIGRILQMHANHREDMEAA
                     FTGDIVAIVGLKHSSTGDTLADPANSIILESISFPTPVISVAVEPKTKADQDKLGTGL
                     AKLADEDPTFVVKYDDETGQTVISGMGELHLDIIVDRLRREFGVDANVGKPQVAYRES
                     IRKPVTKVEGRFIRQSGGHGQFGLVYLDLEPTGQGGGYEFINKIVGGTIPREYIPAVD
                     QGIQEAMESGVLAGYPLVDVRATLMDGSFHEVDSSEMAFKIAGSIGLKEAARRADPVL
                     LEPVMEFEVITPEEFMGEVMGDVTARRGRIERIDDRSGQKA"
BASE COUNT          289 a          539 c          587 g          232 t
ORIGIN      
        1 gtggagcggt ccctgcgcgt gctcgacggc gcgatcgcgg tgttcgacgc cgtcgccggc
       61 gtcgagcctc agacggagac cgtctggcgc caggccgacc gctactcggt cccgcggatc
      121 ggcttcgtga acaagatgga ccgcaccggc gccgacttcg ggcgcacggt cgagatgatg
      181 gtcgagcgct tgcaggcgaa cccgctcgtc ctgcagctgc cctgggggag cgagggcgac
      241 ttccagggcg tgatcgacct cgtcgagatg aaggcgctgc actgggtctc ggagatgggc
      301 gaggagtggg agaccacgga gatccccgcc gagctggcgg atgccgccaa gcgggcgcac
      361 cacgacctgt tcgagaagct cgccgaccac gacgagtggc tgatggagaa gttcgttcac
      421 gaggaggagc ccaccgccga cgagctccgc cgcgcgatcc gacgcgcaac cctgtccggc
      481 gagggcgtgc cggtgctgct gggttcggcg ttcaagaaca aggcgatcca gcctctgctg
      541 gacgcgatcg tcgcctacct gccggcgccg agcgacgtcc cgcccgtcga ggggcatacc
      601 ctcaagggcg agcacgcgga gcgcaagccg gaggactcgg agccgttctc gggcctggtc
      661 ttcaagatca tgtccgaccc cttcgtgggc cggctcacgt acgtccggct ctactcgggc
      721 atcctgcgcg cgggctcgca cgtcgagaac acgaccaagg agcgcaagga gcgcatcggc
      781 cgcatcctgc agatgcacgc gaaccatcgc gaggacatgg aggccgcgtt caccggcgac
      841 atcgtcgcga tcgtcgggct caagcactcg agcaccggcg acacgctcgc cgaccccgcg
      901 aactcgatca tcctggaatc gatcagtttc ccgaccccgg tgatctcggt cgccgtcgag
      961 cccaaaacca aggccgacca ggacaagctg ggcaccggcc tggccaagct cgccgacgag
     1021 gacccgacgt tcgtcgtcaa gtacgacgac gagacgggac agacggtgat ctcggggatg
     1081 ggcgagctcc acttggacat catcgtggac cgcctgcgcc gcgagttcgg cgtggacgcc
     1141 aacgtcggga agccgcaggt ggcgtatcgc gagtcgatcc gcaagccggt cacgaaggtc
     1201 gagggacgct tcatccgtca gagcggtggg cacggacagt tcggcctcgt gtacctggac
     1261 ctcgagccca ccggtcaggg gggcgggtac gagttcatca acaagatcgt cggcggcacg
     1321 atcccgcgcg agtacatccc ggcggtcgac caggggatcc aggaggcgat ggagagcggc
     1381 gtgctcgccg gctacccgct cgtggacgtc cgcgccacgc tgatggacgg gtccttccac
     1441 gaggtggact cgtccgagat ggcgttcaag atcgccggct cgatcggcct caaggaggcg
     1501 gcgcgccgcg ccgaccccgt gctgctcgag cccgtgatgg agttcgaggt gatcacgccc
     1561 gaggagttca tgggcgaggt catgggcgac gtgaccgcga ggcgcggacg gatcgagcgg
     1621 atcgacgacc ggagcggcca gaaggcg
//
LOCUS       DATBRK010000290         4950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_630013, whole genome shotgun sequence.
ACCESSION   DATBRK010000290 DATBRK010000000
VERSION     DATBRK010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630013"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VIH70_08795"
     CDS             complement(<1..1276)
                     /locus_tag="VIH70_08795"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HEY8116498.1"
                     /translation="MRKLQPLVALLVPVALLAGAAYADRDATPRTFAPANDVRATSGA
                     WFCPHGGGRKGWEVRLQVANPGERSATVRVRSLDGDRPDEPETFDVPAGGLVQIPVAS
                     RGRESSSMVEWFGQWVAVGWLAHAGGGEGGVAAEPCAPASGGRWFLPDGTTDVEKDHD
                     YVVVMNPFAREAVFSITLLSERKEPVRHGSLTDVALRPFRSIAFDLGDVVLGEPTVSA
                     LVDVSVGRVAAATLGVVDTGGIRSALGYLGTPPGSLTFPGGADAGRTQLVVMSAGTGN
                     EAARASFGGDILVRGGPPQEFAGLADASMPAASARTFPSTTSGPTSVRFTVSGQDVAA
                     VRRTFGVASDQASVTGALPASSWIVLPTVAGEPSNPGLVLTNPGIEPAVVTLSFLSPG
                     PAEQISVNVPPRSTVEAPEEFLRVAPEAGVRAE"
     gene            complement(1273..4341)
                     /locus_tag="VIH70_08800"
     CDS             complement(1273..4341)
                     /locus_tag="VIH70_08800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8116499.1"
                     /translation="MATTTVDRSPASHDVRSPRVLVVLVTRDAVAWLRGCLESLAAQT
                     HPRLGVVAVDNGSSDGTLQLLRQALGEARVVALGGNRGIAGALTAATQLPAARAADYL
                     LLVHDDAALAPDAVERFLEAAEGIKGVERVGVVGPKIVDWDHPRVLREVGRSTDRFGH
                     PYTPLQDGELDQGQYDRVLEVLFVSSAVMLVSREAWQRTGPFDERFAGHHDDLDFCWR
                     ARLAGFRVLMTPLATARHRDATSRGERREEQPSRSTRYYAERAALASMLKDYGVLTLL
                     WLLPVYVVLGLARMLYLALSRRFEDAFDLLGAWGWNLRHLPGTLRRRIRAQSVRSVAD
                     RRIRPFMASSFRLTRWFERAEEFLDEGLEELDDEELGRPLPRRAATVAAGHPVLLGSI
                     LAIGLGALAVRHFIGPEVLSGGALAAFPTDLGDFFREFGSGVRTTVLGGVQPASPALA
                     GLGAGSWVAFGSTALAQKTLLGAMPALAGIAMYRAMARQTGSPGAAVVAAAAYVLSAT
                     MLWGFSEGRLSFLVVLAVLPLAWDRIDAAFARRAPERPFRFGVGLGVALSIGAAFAPA
                     IVLPVGLFALANLVAGRRRDRGAALVALAALSTALLAFPVALAAWGDPASALASEIGT
                     TDPWSLLRLAPGTGPGTWAVCAFLPVAAIVCFAGAGDPQRGRAWRALLVAVSGTLLAW
                     ASVAGYLPAALTNAPEWIAAAAVAESALVAYGLSTFGKGLERQAFGARQLGVAVLAVV
                     LSVGIAGQALQVTFAEWEVRPGGLPPAWLVIASSAPGPFRILWLGDPDGDRFPAPGGD
                     PISLAEAGERTVRFGLTDREGATALDEGRARSGRGYDELHDVLERLLDGGTRHAGALL
                     GPFGVRFVIAGEGDLSPGVAERLSEQLDLDRIPAGGLTIYRNAAALPTAFVSSNAPVP
                     DRSDPAEVEASPDVIARELGGGGASFTATADAPGHVVVTQQFDAGWRVENGGEVLAPF
                     PGYGWAIAAPVQEGTVAVRFTDQWVRTVELFLLAVMWGVALWMTRKPGSA"
     gene            complement(4448..4786)
                     /locus_tag="VIH70_08805"
     CDS             complement(4448..4786)
                     /locus_tag="VIH70_08805"
                     /inference="COORDINATES: protein motif:HMM:NF019028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HEY8116500.1"
                     /translation="MSPAKKRSTAKRKTAARKPAAKKTAARKTTRKRKTAARKPAAKK
                     TTRKKTTRRKPAAKKTAARKTTRRKTTRRKTAARKPAAKKTTRRKTTRRKTAAKKTAA
                     RRPARRRTAK"
     gene            complement(4810..>4950)
                     /locus_tag="VIH70_08810"
     CDS             complement(4810..>4950)
                     /locus_tag="VIH70_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY8116501.1"
                     /translation="REAKRICADCPVRIECLNYALRRDERYGVWGGMSERERRRLKRM
                     AS"
BASE COUNT          721 a         1855 c         1755 g          619 t
ORIGIN      
        1 cctccgccct caccccggcc tcgggggcga cccggaggaa ctcctcgggc gcctccacgg
       61 tcgagcgcgg cggcacgttg acgctgatct gctcggcggg ccccggggac aggaagctga
      121 gcgtgacgac cgcgggctcg atgccggggt tcgtcagcac caggccgggg ttcgagggtt
      181 cccctgcgac cgtgggcagc acgatccacg acgacgcggg cagggcccca gtgacggacg
      241 cctggtccga ggccactccg aacgtccgcc ggacggccgc cacgtcctgc cccgacaccg
      301 tgaagcgcac cgaggtcggg cccgaggtcg tggacgggaa ggtccgagcc gaggcggcgg
      361 gcatgctcgc gtccgcgaga ccggcgaact cctgcggcgg tccgccccgg accaggatgt
      421 cgccgccgaa cgaggcgcgc gccgcctcgt tcccggtccc ggcgctcatc acgaccagct
      481 gggttcggcc cgcgtccgcg ccgccgggga aggtgagcga tcccggcggc gtcccgagat
      541 accccagtgc ggacctgatc ccgcctgtgt ccacgacgcc gagcgtcgcg gccgcgaccc
      601 gtccgacgga gacgtcgacg agcgcggaga ccgtgggttc gccaaggacc acgtcgccga
      661 ggtcgaacgc gatgctgcgg aaggggcgaa gggcgacgtc ggtgagcgag ccgtgtcgca
      721 ccggttcctt gcgttcggac agcagggtga tcgagaagac ggcctcgcgc gcgaaggggt
      781 tcatcacgac gacgtagtcg tggtccttct cgacgtccgt cgtcccgtcc ggcaggaacc
      841 agcgtccccc ggacgccggg gcgcacggct cggcggcgac gcccccctcg cccccgccgg
      901 cgtgggcgag ccaccccacg gccacccact ggccgaacca ctcgaccatc gacgagctct
      961 cgcggcctcg tgatgcgacc gggatctgca cgagtccgcc ggccggcacg tcgaacgtct
     1021 ccggctcgtc gggcctgtcc ccgtcgaggg accggacgcg gaccgtcgcg ctccgctccc
     1081 ccgggttcgc cacctgcagc cggacctccc accccttccg accgccgccg tgtgggcaga
     1141 accacgcacc ggaggtcgca cggacgtcgt tggccggcgc gaacgttcgg ggcgtggcgt
     1201 cgcggtcggc gtacgccgcg ccggccagca gcgccaccgg gacgagaagc gcgaccaggg
     1261 gctgcagctt cctcacgctg accccggctt ccgcgtcatc cagagggcga cgccccacat
     1321 gaccgcgagc aggaagagct cgaccgtgcg gacccattga tccgtgaagc gaacggccac
     1381 cgtcccctcc tgcacgggcg cggcgatcgc ccagccgtag ccggggaagg gcgccagcac
     1441 ctcgccgccg ttctcgaccc gccatcccgc gtcgaactgt tgcgtcacca cgacgtggcc
     1501 cggcgcatcg gcggttgcgg tgaaggacgc gcctccgcct ccgagctcgc gcgcgatcac
     1561 gtcgggtgag gcttcgacct cggccgggtc ggaacgatcg ggaaccggcg cgttggacga
     1621 tacgaacgcg gtcggcaggg cggccgcatt ccgatagatc gtcaatccgc cggccgggat
     1681 acggtccaga tccagctgct cggacaggcg ttccgcgacg cccggcgaga gatcgccctc
     1741 cccggcgatc acgaaccgga ccccgaacgg gccgagcagc gcgccggcat gccgggtgcc
     1801 gccgtcgagc agccgttcga ggacatcgtg gagctcgtcg tagcccctgc ccgaccgcgc
     1861 gcgcccctca tcgagcgcgg tcgccccctc acggtcggtg aggccgaacc gcaccgtccg
     1921 ctcgccggcc tcggccaacg agatcgggtc gcctccggga gcggggaagc gatcgccgtc
     1981 cggatcgccg agccacagga tccggaacgg ccccggcgcg ctcgaggcga tcaccagcca
     2041 cgcgggcggg agtccgcccg gccgcacctc ccactcggcg aatgtgacct ggagggcctg
     2101 cccggcgatg ccgaccgaga gcaccacggc gagaaccgcc accccgagct ggcgagcgcc
     2161 gaacgcctgc cgttccagcc ccttgccgaa ggtcgagagc ccgtacgcga cgagcgcgct
     2221 ctcggccacg gcggccgccg cgatccactc gggcgcgttg gtgagcgccg cgggtagata
     2281 cccggctacc gacgcccacg cgaggagggt ccccgagacc gccaccagca gcgctcgcca
     2341 ggcccggccg cgctgcggat ctcccgcgcc ggcgaagcac acgatcgcgg cgaccgggag
     2401 gaacgcacag acggcccacg tcccgggtcc cgtccccgga gcgagccgga gcagcgacca
     2461 ggggtccgtg gtcccgatct cggaggcgag cgccgaggcg ggatcgcccc acgcggcgag
     2521 cgcgacgggg aacgcgagca acgccgtcga gagcgcggcg agcgcgacca gcgcggcccc
     2581 gcgatcgcgt cgacggccgg cgacgaggtt tgcgagcgcg aacagcccga cgggcaggac
     2641 gatcgccggg gcgaacgccg cgccgatcga caacgccacg cccagcccga cgccgaaccg
     2701 gaacggacgc tccggcgcgc gccgggcgaa cgcggcgtcg atccggtccc acgcgagtgg
     2761 gaggaccgcg agcacgacca ggaaggagag ccgcccctcg gagaagcccc agagcatcgt
     2821 cgcggagagc acgtacgcgg ccgctgccac cacggccgcg cccggcgagc ccgtctggcg
     2881 cgccatcgcc cgatacatcg cgatgcccgc gagcgcgggc atcgcgccga gcagggtctt
     2941 ctgcgcgagc gcggtgctcc cgaatgccac ccacgacccc gccccgagcc cggcgagggc
     3001 cgggctggcc ggctggacgc cgccgagcac cgtcgttcgg acccccgagc cgaactcgcg
     3061 gaagaagtcg ccgaggtcgg tggggaacgc ggcgagcgcc ccgcccgaaa gcacctcggg
     3121 accgatgaag tggcgcaccg cgagcgcccc gagcccgatc gcgaggatcg acccgagcag
     3181 gaccgggtgg ccggccgcca cggtggccgc gcggcggggc agcgggcgcc cgagctcttc
     3241 gtcgtcgagc tcctccagac cctcgtccag gaactcctcc gcccgctcga accacctggt
     3301 gagacggaaa gaggacgcca tgaacgggcg gatcctgcgg tcggctaccg aacggacgga
     3361 ttgggctcgg atccttcggc ggagcgtccc gggaaggtgc cggaggttcc acccccaggc
     3421 tccgagcagg tcgaacgcgt cctcgaagcg ccttgacagg gccaggtaga gcatccgggc
     3481 gaggcccagc acgacgtaca ccggcaggag ccacagcagc gtgaggaccc cgtagtcctt
     3541 gagcatcgag gccagcgcgg cgcgctcggc gtagtagcgg gtgctcctcg agggctgctc
     3601 ctcgcgtcgt tcgccccggg aggtcgcgtc ccggtggcgg gcggtggcga ggggggtcat
     3661 caggacccgg aacccggcca ggcgggcccg ccaacagaag tcgagatcgt cgtgatgacc
     3721 ggcgaagcgc tcgtcgaacg gccccgtccg ctgccacgcc tcgcgcgaga ccagcatgac
     3781 ggccgaggag acgaacagga cctcgagcac ccggtcgtac tgcccctggt cgagctcgcc
     3841 gtcctggagc ggggtatagg ggtgtccgaa ccggtcggtc gagcgcccca cctcccgaag
     3901 gaccctcggg tggtcccaat cgacgatctt cgggccgacg acgccgacgc gttcgacgcc
     3961 cttgatgccc tcggcggcct cgaggaagcg ttcgacggca tcgggcgcga gcgccgcgtc
     4021 gtcgtgcacc agtaagaggt agtcggcggc ccgggcggcc gggagctggg tcgcggcggt
     4081 cagcgcgccc gcgatgcccc ggttgccccc gagcgccacc acccgcgcct caccgagcgc
     4141 ctgtcgcagg agctggagcg tgccgtcgga ggaaccgttg tccacggcga caacgcccaa
     4201 gcgcggatgg gtctgcgcgg cgagcgactc gaggcaaccc cgcagccacg cgacggcgtc
     4261 gcgcgtgacg agcacgacga gcaccctggg cgagcggacg tcgtgcgaag caggagaacg
     4321 atcgacggtt gtcgtggcca tgaagtccgc cctgaggcgg cgcgcagcct agcacgccgg
     4381 tgttcgaaca cgcggaaggg gggcgatgcc ccccttccga gcaccaacgg cgaaccgaag
     4441 gctaccgcta cttcgcggtc cgcctcctcg ccggtctgcg tgccgcagtc ttcttggccg
     4501 cggtcttcct gcgcgtggtc tttctccgcg tcgtcttctt ggccgccggc ttgcgcgcgg
     4561 ccgtcttcct gcgcgtggtc tttctccgcg tcgtcttgcg cgcggcggtc ttcttcgccg
     4621 ccggcttcct ccgcgtggtc ttcttcctcg tcgtcttctt cgctgcgggc ttccgcgcgg
     4681 cggtcttcct cttacgcgtg gtcttgcgcg cggcggtctt cttcgccgcc ggcttgcgag
     4741 ccgccgtctt gcgctttgcg gtcgatctct tcttcgcggg agacatcggt cacccccttt
     4801 ccatcgatct caggacgcca tgcgcttcag gcgtcgccgt tcgcgttcgc tcatccctcc
     4861 ccacaccccg taccgctcgt cgcggcgaag ggcgtagttg aggcactcga tgcgaaccgg
     4921 acaatcggcg cagatccgct tggcctctct
//
LOCUS       DATBRK010000291         8822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1923119, whole genome shotgun sequence.
ACCESSION   DATBRK010000291 DATBRK010000000
VERSION     DATBRK010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923119"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..550)
                     /gene="nrdR"
                     /locus_tag="VIH70_08815"
     CDS             complement(77..550)
                     /gene="nrdR"
                     /locus_tag="VIH70_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY8116502.1"
                     /translation="MRCPWCGHDGDKVVDSRPAERGDAIRRRRQCLSCGRRFTTYERV
                     EQLALVVVKRDGTSEPWDREKMVSGIRKALVNRPVTDEQFAALVSRIEGRLRRKGPEI
                     TSQRIGLEVLGNLQKLDQVAYVRFASVYKDFQGIGDFEREFGLMLQKKESKARSR"
     gene            complement(610..939)
                     /locus_tag="VIH70_08820"
     CDS             complement(610..939)
                     /locus_tag="VIH70_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8116503.1"
                     /translation="MSGDRRVNEVFSALSDPTRRAVIRSLSEDGPSTVTELAGRLPVT
                     RQAVTKHLEALEDAGLVASDARGRGARFRLTPRPMTEAMRWMADVGGEWDERLEALRT
                     HLARRRR"
     gene            complement(936..1298)
                     /locus_tag="VIH70_08825"
     CDS             complement(936..1298)
                     /locus_tag="VIH70_08825"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8116504.1"
                     /translation="MRDERVTRSVVLPAGRDEVWATLTTPEDLSAWLGEVVELEPEPG
                     GAIVLREQGSTRRGMVEAAEPGRILSFRWRRLAGAGVSLEVGGATRVTFVLESDPEGT
                     RLTVTEEPVLLATAGLGA"
     gene            1410..2069
                     /locus_tag="VIH70_08830"
     CDS             1410..2069
                     /locus_tag="VIH70_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY8116505.1"
                     /translation="MTYRSGPLVPTVVEESARGERAFDIYSRLLRERIVFLVEPIDDA
                     VATTVVAQLLFLDSVEPGKDIALYVNSPGGASTALFAIYDTMRFLRSDFATYCLGQAA
                     SAAAVILAAGTLGKRFALPNSRVLLHQPHGGIEGQSADIEIHAAEIARQRRRVEEILA
                     EHTGQTVERIHADTERDFILGPEEARDYGVVDQVVASRDLVRVPRPIELRPQRAETTS
                     V"
     gene            complement(2042..2332)
                     /locus_tag="VIH70_08835"
     CDS             complement(2042..2332)
                     /locus_tag="VIH70_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8116506.1"
                     /translation="MTRTRVRRRRLTLTISLALVAVAWAGPAVRALRSPDTPARVSRT
                     SYVVRQGDTLWTIARELAPGSDPRPVVDLLSAMNGVDPGRLVPGQTLVVSAL"
     gene            2443..3087
                     /gene="lexA"
                     /locus_tag="VIH70_08840"
     CDS             2443..3087
                     /gene="lexA"
                     /locus_tag="VIH70_08840"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY8116507.1"
                     /translation="MAPIEPSEAPETGPLTARQHRIVEFIATTVRERGYPPTVREIGE
                     AVGLTSSSSVHAQLANLERRGLLTKDPTKPRAMTLSDANRAEGVVVPLVGRIAAGTPV
                     LADQNVEDHLVVPMGYAGDAEHFALSVTGDSMVDAGILDGDVVVVRGQDTAEDGDVVA
                     VLLPGPAEDEATVKRLRRRRGKVVLVPENPAMDPFDMDPDGRILGKVVAVLRKL"
     gene            3145..4125
                     /locus_tag="VIH70_08845"
     CDS             3145..4125
                     /locus_tag="VIH70_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY8116508.1"
                     /translation="MPDTTALERQITSQPEQLEAVLAEPLATSAAERLRDANRIWLVG
                     TGTSQHAAELGAQMLQQARRDARAVSSMSFANRPPFARGDGVILISHNAGAETSFAGA
                     SWTAATRAGLPVVPVTRRGGSLPDAIETVEKETSHTYTASYTAALVQIARLAHELGAE
                     AYAPSALEAVPPAVRAAIDRPGTDGVAQPERLLVLTGEGPGAVTAREGALKIREAARF
                     PAEGYDVEYLLHGHAVPLNASDHLVTLAPPDTQGLTAGVEGAGRASGIPVTRIDEPAD
                     LPSPLEQIPLTVRLQVLALRYALERGQNPDVAIEGPWDDPELWAIGSPGS"
     gene            complement(4130..4594)
                     /locus_tag="VIH70_08850"
     CDS             complement(4130..4594)
                     /locus_tag="VIH70_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8116509.1"
                     /translation="MDPVPFSPEQPAAFAEALAEGSSRRAPGLGELDVAPMVPVAFDP
                     DLGSCRDELRPLLALYAGGMGSAGANFYKDLVTRYGFGDAANAIQEAFLDGRRTEAAG
                     LVPDAMIDELCLVGPVDHVRERLDAWREAGVTTLLAKARDVRTVRALAEAAA"
     gene            complement(4624..5142)
                     /locus_tag="VIH70_08855"
                     /pseudo
     CDS             complement(4624..5142)
                     /locus_tag="VIH70_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            complement(5200..6618)
                     /gene="hflX"
                     /locus_tag="VIH70_08860"
     CDS             complement(5200..6618)
                     /gene="hflX"
                     /locus_tag="VIH70_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY8116510.1"
                     /translation="MAQNGRRRPGGSGGSRLEGERASGSPPRGRGTHETSTLESPLAA
                     TWRPRVPEKAVLVGVGRGIEEADLDELAALADSAGAEPVERVLQTRQDPNPATFVGKG
                     KINEVQDAVHRTGAEAVILDDELTPGQLRSLEERLKVKVIDRTALILDIFALHARSRE
                     GKAQVELAQLTYLLPRLRGWGEAMSRLGGGIGTRGPGETKLEVDRQHIKRRISKLRKD
                     LKDLARTRDVKRSSRERSGIPQVAITGYTNAGKSTLMNALTASDVVVANQLFATLDPT
                     VRKIELPSGRAATISDTVGFVSKLPHELVEAFRSTLEEVARSTLIVHVADASSPDVIE
                     QIDAVREVLAEIGAVHIPEVLALNKIDRLSQTERARVLGRIDGDAVPISARTGDGLDD
                     LMERVERSLPRFPMDVTILVPWGREDVTAMLYRSAEVLSEDAREDGTLVHARVGEREF
                     AAVATFRVEPAVAPSARDRAAG"
     gene            complement(6629..7783)
                     /locus_tag="VIH70_08865"
     CDS             complement(6629..7783)
                     /locus_tag="VIH70_08865"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEY8116511.1"
                     /translation="MRVAKRVESLPPYLFAELDRKLAAKRAEGVDVISLGVGDPDLPT
                     PEHVVEAMREAVLDPSTHRYPSYYGTPEFRRAVASWYDRRFGVELDPETEIMAMIGSK
                     EGIGHIAFAFVDPGDEALIPDPGYPVYGVSTRLAGGTPVSLPMPEDEGFLPDLDAAPV
                     TGRTKAIWLNFPSNPTAAVAELPTFEAATAFAREHDLILLHDAAYSEITFDGFVAPSA
                     LQAQGAKEVALEFGSASKFYNMTGWRIGWAAGSAEAIRALGVVKTNLDSGQFTAIQRA
                     AIAALEGPPEHLDELRATYRRRRDLVVETLNGLGWSLKPPLGSCYVWAPVPGGDTSAS
                     FADRLLDTTGVFVAPGSGYGTRGEGFVRFSLTVPDDRLAEAMERVGRALA"
     gene            complement(7780..8646)
                     /gene="dapF"
                     /locus_tag="VIH70_08870"
     CDS             complement(7780..8646)
                     /gene="dapF"
                     /locus_tag="VIH70_08870"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY8116512.1"
                     /translation="MDELRFWKYQGTGNDFVLLEDVAGRLELGEELARRLCDRRFGVG
                     ADGVIRVAPSSEADFFMDHRNADGSRAQMCGNGIRCLGKLVYDRGLIDRTEIRVDTRS
                     GIKTLALHVGDGGVDSVTVGMGPARFARGALPMAGNPDEPFVGEPFEVDGRSYKATAL
                     SMGNPHLVLFVEEDPDDVDVHRLGPLVEHDERFPERTNVEFVAVQGDGVKVRVWERGA
                     GETMACGTGACAAVVAANEAGLVPAKAPARFPGGVLRIERLPGDQVLLTGPAVRVAEG
                     VVDPSWLEAVSG"
     gene            complement(8639..>8822)
                     /locus_tag="VIH70_08875"
     CDS             complement(8639..>8822)
                     /locus_tag="VIH70_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY8116513.1"
                     /translation="LDAAIAGTVKRTRALARRQLAFFRRDPRIRWFEAGQAGAGPLVE
                     DVVAYLSWQVRRPADG"
BASE COUNT         1247 a         3303 c         3061 g         1211 t
ORIGIN      
        1 cgccggtcga cgcagtcaag cgttcgatca aggatatctt gtggtcggat cgagtgacat
       61 ccacaagata tagtggtcag cgcgagcggg ccttggactc cttcttctgg agcatgaggc
      121 cgaattcacg ctcgaagtcg ccgatcccct ggaagtcctt gtagacgctc gcgaaccgca
      181 cgtaggcgac ctgatcgagc ttctggaggt tgcccagcac ctccagcccg atccgctgcg
      241 aggtgatctc ggggcccttg cggcgcaggc gcccctcgat cctcgagacg agcgcggcga
      301 actgctcgtc ggtgacgggc cggttgacca gggccttccg gatgcccgag accatcttct
      361 cccggtccca gggctcgctc gtcccgtctc gtttgacgac caccagggcg agctgctcca
      421 cccgctcgta ggtggtgaac cggcgtccgc aagacaggca ctggcgccgg cgccggatcg
      481 cgtcgccgcg ttcggcgggc cgggagtcga ccaccttgtc cccgtcgtgc ccgcaccacg
      541 gacaccgcat caacgatcac cccaacatct agtggaccga ggcacggtaa ccgcgagatg
      601 tggggtgcgt caacgacgac ggcgggcgag gtgcgtccgg agcgcctcca gacgctcgtc
      661 ccactcgcca cccacgtcgg ccatccaccg catggcctcg gtcatcggac gcggggtgag
      721 ccggaaccga gccccgcgac ctcgggcgtc ggacgcgacg aggccggcat cctcgagcgc
      781 ctcgagatgc ttggtcacgg cctgccgcgt cacggggagg cggccggcga gctcggtcac
      841 ggtactcggg ccgtcctcgg acaggctgcg gatcacggcc cggcgcgtcg gatccgagag
      901 cgccgagaac acctcgttca cccgacgatc gccgctcacg ccccgagccc cgccgtcgcg
      961 aggagtaccg gctcctcggt gaccgtcagg cgtgtgcctt cggggtcgga ctccaggacg
     1021 aacgtcacgc gcgtggctcc cccgacctcg agcgagacgc ccgccccggc cagccgccgc
     1081 catcggaacg acaagatcct gcccggctcg gcggcctcga ccatccctcg ccgcgtcgat
     1141 ccctgttcgc ggaggacgat cgccccgccg ggctccggct cgagctccac cacctcgccc
     1201 aaccaggccg acagatcctc cggggtcgtg agcgtcgccc acacctcgtc ccggcccgcc
     1261 ggcagcacca ccgacctcgt gacccgttcg tcgcgcaccc agacatcgta cgcaaccgaa
     1321 gggttgccgg cacccaggaa cgcgggctac catccactcg gcaacccgct ggttgctgag
     1381 tcgtggtcga tcgaacgagg aggatcccga tgacctaccg atccggaccg ctggtcccca
     1441 ccgtcgtgga agagagcgct cggggcgagc gagccttcga catctactcc cggctcctgc
     1501 gcgagcggat cgtgttcctc gtcgagccca tcgacgatgc ggtcgcgacc accgtggtcg
     1561 cgcagctcct gttcctcgac tcggtggagc cgggcaagga catcgcgctg tacgtgaaca
     1621 gcccgggcgg cgcgtcgacg gcgctgttcg cgatctacga cacgatgcgg ttcctcaggt
     1681 ccgacttcgc gacctactgc ctgggtcagg cggcctcggc cgcggcggtc atcctggcgg
     1741 cgggcaccct cggcaagcgg ttcgcgctgc cgaactcccg ggtcctgctc caccagcccc
     1801 acggtgggat cgaggggcag tcggccgaca ttgagatcca cgcggccgag atcgcccggc
     1861 agcgtcgtcg cgtcgaggag atcctggccg agcacacggg gcagaccgtc gaacggatcc
     1921 acgcggacac cgagcgcgac ttcatcctcg gtcccgagga ggcacgcgac tacggcgtgg
     1981 tcgaccaggt cgtcgccagc cgcgacctcg tgcgcgtccc tcgcccgatc gagctccggc
     2041 ctcagagggc cgagaccacg agcgtctgac caggcacgag ccggcccggg tccacgccgt
     2101 tcatcgccga gagcaggtcg acgaccggcc gggggtcgct cccgggcgcg agctccctgg
     2161 cgatcgtcca cagcgtgtcg ccctgccgga ccacgtacga ggtccgagag acccgcgcgg
     2221 gcgtgtccgg cgaccggagt gcccgaacgg ccgggccggc ccaggcgacc gccacgagcg
     2281 cgagcgagat cgtcagggtc agcctgcgtc ggcgaacacg cgttcgagtc acgagcccat
     2341 cctaccgaac atgtgttcgg cgtcaagcac ggaaccgaac atatgtttga acgccccttc
     2401 ctctcgtgct agcgtcctgc gaggctagaa ggaggcaccg acatggctcc catcgaaccg
     2461 tccgaggcac ccgagaccgg gccgctcacc gcccggcagc accggatcgt ggagttcatc
     2521 gccaccaccg tgcgggagcg cggctatccc cccaccgtgc gcgagatcgg cgaggccgtg
     2581 ggcctgacgt cgtcctcgag cgtccacgca cagctcgcga acctggagcg ccggggcctc
     2641 ctcacgaagg atccgaccaa gccccgggcc atgacgctgt ccgacgccaa ccgcgccgag
     2701 ggcgtcgtcg tcccgctcgt tgggcggatc gcggcgggca ccccggtgct cgccgaccag
     2761 aacgtcgagg accacctcgt ggtcccgatg ggctacgcgg gcgacgccga gcacttcgcg
     2821 ctcagcgtca cgggagattc gatggtcgac gccggcatcc tcgacggcga tgtcgtggtc
     2881 gtccggggtc aggacacggc cgaggacggc gacgtggtcg ccgtgctgct cccggggccc
     2941 gccgaggacg aggccacggt caagcgcctg cgccgccggc gcggcaaggt cgtgctcgtc
     3001 ccggagaacc cggcgatgga cccgttcgac atggacccgg acggccggat cctcggcaag
     3061 gtcgtcgcgg tcctgcgaaa gctgtagcgc tcgcgcgccg tcacggcgtc gtctaggctc
     3121 gccggaccga cccggaggtg acccttgccc gacaccacgg cgctcgaacg acagatcacg
     3181 tcccagcccg agcagctcga ggccgtcctc gccgagccgc tcgcgacctc ggccgccgag
     3241 cgtctccgcg acgcgaaccg gatctggctc gtcggcacgg ggacgagcca gcacgcggcg
     3301 gagctcggcg cacagatgct gcagcaggcg aggcgcgatg cccgcgcggt gagttcgatg
     3361 tcattcgcga accgtccgcc gttcgcgcgg ggcgacggcg tcatcctgat cagccacaac
     3421 gcgggcgccg agacgtcgtt cgccggcgcg tcctggacgg cggccactcg ggcgggcctt
     3481 cccgtggtgc ccgtcactcg gcgcggcggc tcgctccccg acgcgatcga gaccgtcgag
     3541 aaggagacct cgcacaccta cacggcgagc tacacggcgg cgctcgtgca gatcgcgcgg
     3601 ctcgcccacg agctcggtgc cgaggcgtac gcgccgtccg cgctcgaggc ggtgcccccc
     3661 gcggtgcgag cggcgatcga ccggccgggg accgatggtg tcgcgcagcc cgagcgcctg
     3721 ctggtcctga ccggcgaggg cccgggggcg gtgaccgccc gcgagggcgc gctcaagatc
     3781 cgcgaggcgg cgaggttccc cgccgagggg tacgacgtcg agtacttgct gcacgggcac
     3841 gcggttccgc tcaacgcgtc cgatcatctg gtgacgctgg cgccgccgga cacgcagggc
     3901 ctgacggccg gggtcgaggg cgccggccga gcctcgggca tcccggtcac ccggatcgac
     3961 gagccggcgg acctcccgtc gccgctcgaa cagatcccgc tcacggtcag gctccaggtg
     4021 ttggcgctcc ggtatgcgct cgagcgcggg cagaaccccg acgtagcgat cgaggggccg
     4081 tgggacgacc ccgaactctg ggcgatcggc tcccccggct cctgacgggc tacgcggcgg
     4141 cttcggcgag cgcgcggacg gtccgcacgt cgcgcgcctt cgcgagcagg gtggtgacgc
     4201 cggcctcgcg ccacgcgtcg agccgctcgc ggacgtggtc gacagggccg accaggcaga
     4261 gctcgtcgat catcgcgtcg gggaccaggc ccgcggcctc ggtccggcgc ccgtcgagga
     4321 acgcctcctg gatcgcgttc gccgcgtcgc cgaacccgta tcgggtcacg aggtccttgt
     4381 agaagttcgc gccggccgag cccatcccgc cggcgtagag cgcgagcagc ggtcgcagct
     4441 cgtcccggca gctcccgagg tcggggtcga aggcgaccgg caccatcggc gcgacgtcca
     4501 gctccccgag gccgggtgcg cgacgactcg agccctcggc gagcgcctcg gcgaacgccg
     4561 ccggctgctc gggcgagaaa ggaacgggat ccagccctgc gcgatctcgg ccgcgagcgc
     4621 gacgttcctc ggtcccatcg cggcgagata gatcgggacg tcgggccgca gcggccggac
     4681 gttgggcttg agcgccttgc ccccgccgcc ggggagcggc agcgggtagt tcggcccggg
     4741 cgaggtcacc ggcgcctcgc gtcgcagcac gccccgcacg atgtcgacgt actcgcgcgt
     4801 gcgtgcgatg ggcgcgtcga acgacgctcc gtgccacccc tccgcgacct gcggccccga
     4861 gacgccgagc ccgagccgca tccgcccgtc gctcaggtgg tcgagcgtga gcgccgccat
     4921 cgcggcggcg gccggcgacc ttgccggcat ctggagcacc ccggccccga gcccgatccg
     4981 gtcggtgtgc gcggcgatcc acgagagcag gacaacgccg tccgagcccc aggtctccga
     5041 ggtccacacg gagtcgaacc cggcgcgttc ggcctccacc gcgagctcgg cgacatcgtg
     5101 gggacgtccc tcccagtgcc cgacgtggat cccgatccgc acgcgcggag ggtacccgag
     5161 cgcgcgtccc gcggagcgag cgaccggcag cccgggacgt cagcccgcgg cgcggtcccg
     5221 cgccgaaggt gcgacggccg gttccacgcg gaaggtcgcc accgctgcga actcgcgctc
     5281 ccccaccctg gcgtggacga gcgtgccgtc ctcgcgcgcg tcttccgaca ggacctcggc
     5341 gcttcggtac agcatcgcgg tcacgtcctc gcggccccat gggaccagga tcgtgacgtc
     5401 catcgggaag cgcgggagcg agcgctcgac gcgttccatc aggtcgtcga gcccgtcgcc
     5461 ggttcgcgcg ctgatcggga ccgcgtcgcc gtcgatgcgt cccagcaccc gggcgcgttc
     5521 ggtctgggac aggcggtcga tcttgttgag cgccagcacc tcggggatgt gcaccgcgcc
     5581 gatctcggcg agcacctcgc ggacggcgtc gatctgctcg atcacgtcgg gcgaggacgc
     5641 gtcggcgacg tgcacgatca gcgtcgagcg cgccacctcc tcgagcgtgg agcggaaggc
     5701 ctccaccagc tcgtgcggga gcttgctcac gaaccccacc gtgtccgaga tcgtggcggc
     5761 gcggcccgac ggcagctcga tcttccgcac ggtcgggtcg agcgtcgcga agagctggtt
     5821 cgccaccacc acgtcggagg ccgtcagcgc gttcatcagg gtggacttgc cggcgttcgt
     5881 gtacccggtg atcgcgacct gcgggatgcc cgagcgctca cggctcgagc gcttgacgtc
     5941 gcgggtgcgc gcgaggtcct tgaggtcctt gcggagcttc gagatccgtc gcttgatgtg
     6001 ctgccggtcg acctcgagct tggtctcgcc gggcccgcgc gtgccgatgc caccgccgag
     6061 ccggctcatc gcctcgcccc agccgcggag ccgcggcaac aggtacgtga gctgcgcgag
     6121 ctcgacctgc gccttgccct cccggctccg cgcgtgcagc gcgaagatgt cgaggatcag
     6181 cgcggtccgg tcgatcacct tgaccttgag ccgctcctcc aggctgcgga gctgccccgg
     6241 cgtgagctcg tcgtccagga tcaccgcctc ggcgccggtg cgatgcaccg cgtcctgcac
     6301 ctcgttgatc ttgcccttgc cgacgaaggt ggcggggttc gggtcctgac gggtctggag
     6361 cacgcgctcc acgggctccg caccggccga atccgcgagc gccgcgagct cgtccaggtc
     6421 ggcctcctcg atcccgcgac cgacgccgac cagcaccgcc ttctcgggga cgcggggacg
     6481 ccaggtcgcc gcgagcggcg actcgagcgt gctggtctcg tgcgtgcctc ttcctctcgg
     6541 tgggctaccg ctcgcgcgtt cgccctcgag cctcgagccc cccgaacccc ccggcctccg
     6601 acgaccgttc tgcgccatca gcggcccgtc acgccagcgc ccggccgacg cgttccatcg
     6661 cctcggcgag ccggtcgtcg gggacggtga gcgagaaccg cacgaacccc tcgccgcgcg
     6721 tgccgtaccc gctcccgggc gcgacgaaca cgcccgtcgt gtcgagcagc cggtcggcga
     6781 acgaggccga cgtgtcgccg cccggcaccg gcgcccagac gtagcacgag ccgagcggcg
     6841 gcttgagcga ccacccgagc ccgttcaggg tctccaccac caggtcacgc cggcgccggt
     6901 aggtggcccg gagctcgtcg aggtgctcgg gcggcccctc gagcgcggcg atcgccgcgc
     6961 gctggatcgc ggtgaactgg ccgctgtcga ggttcgtctt cacgacgccg agcgcgcgga
     7021 tggcctcggc cgagccggcc gcccacccga tccgccatcc ggtcatgttg tagaacttcg
     7081 acgccgagcc gaactcgagc gcgacctcct tcgcgccctg tgcctgcagg gccgagggcg
     7141 cgacgaaccc gtcgaacgtg atctcgctgt acgcggcgtc gtgcagcagg atcaggtcgt
     7201 gctcgcgcgc gaacgcggtc gcggcctcga acgtcgggag ctcggccacg gcggctgtgg
     7261 ggttgctcgg gaagttcagc cagatcgcct tggtgcgacc ggtcaccggc gcggcgtcga
     7321 ggtccgggag gaacccctcg tcctcgggca tcgggagcga gaccggcgtg ccgcccgcca
     7381 gcctcgtcga gacgccgtac accgggtagc cggggtcggg gatcagcgcc tcgtcgcccg
     7441 ggtcgacgaa cgcgaacgcg atgtgcccga tcccctcctt cgagccgatc atcgccatga
     7501 tctcggtctc ggggtcgagt tcgacgccga atcgacggtc gtaccagctc gcgaccgccc
     7561 gccggaactc gggcgtgccg tagtagctcg ggtagcggtg cgtcgagggg tcgaggaccg
     7621 cctcacgcat cgcctcgacg acgtgctcgg gagtcggcag gtccgggtcg cccacgccga
     7681 gcgagatcac gtccacgccc tcggcgcgct tggccgcgag cttgcggtcc agctcggcga
     7741 acaggtacgg gggcagcgat tcgacgcgct tggcgaccct caacccgaga ccgcctccag
     7801 ccacgacggg tcgacgacgc cctccgcgac gcggacggcg ggacctgtca gcagcacctg
     7861 gtcgcccggg agccgttcga tccgcaggac gccgccgggg aatcgggcgg gcgccttcgc
     7921 gggcacgagg cccgcctcgt tcgccgcgac gaccgcggcg cacgcacccg tgccgcacgc
     7981 catcgtctcg ccggcgccgc gctcccagac ccggaccttc accccgtcgc cctggacggc
     8041 gacgaactcg acgttggtcc gctcggggaa tcgctcgtcg tgttcgacaa gcgggccgag
     8101 ccggtggacg tcgacgtcgt ctgggtcctc ctcgacgaac agcaccagat gcgggttgcc
     8161 catcgacagc gcggtggcct tgtagctgcg cccgtcgacc tcgaacggct cccccacgaa
     8221 cggctcgtcc gggttgccgg ccatcggcag cgcgccgcgc gcgaaccggg ccggccccat
     8281 cccgacggtc accgagtcga cgccaccgtc cccgacgtgc agcgcgagcg tcttgatccc
     8341 cgaacgcgtg tcgacgcgga tctcggtgcg gtcgatcagg ccgcggtcgt acaccagctt
     8401 gcccaagcag cggatcccgt tgccgcacat ctgggcccgg ctgccgtccg cgttgcggtg
     8461 gtccatgaag aagtcggcct cggaggacgg cgcgacccgg atcacaccgt cggcgccgac
     8521 cccgaaccgc cggtcgcaca ggcgtcgcgc gagctcctcg ccgagctcga ggcgcccggc
     8581 gacgtcctcg agcaggacga agtcgttgcc cgtcccctgg tacttccaga aacggagctc
     8641 atccatccgc gggcctccgg acctgccacg acagatacgc tacgacgtcc tcgacgagcg
     8701 gcccggcccc ggcctgcccc gcctcgaacc agcggatcct cgggtcccgc cggaagaagg
     8761 cgagctgccg gcgcgcgagc gccctcgtgc gcttcaccgt gcccgcgatc gccgcgtcga
     8821 gc
//
LOCUS       DATBRK010000292         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_630028, whole genome shotgun sequence.
ACCESSION   DATBRK010000292 DATBRK010000000
VERSION     DATBRK010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630028"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            530..673
                     /locus_tag="VIH70_08880"
                     /pseudo
     CDS             530..673
                     /locus_tag="VIH70_08880"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
     gene            complement(723..1580)
                     /locus_tag="VIH70_08885"
     CDS             complement(723..1580)
                     /locus_tag="VIH70_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8116514.1"
                     /translation="MASISERESREIEAANASGTTPVVFIHGLWLLPSSWANWAEFFK
                     QAGYAPLTPDWPDDPETVEEARANPDVLAKKTLKQVADHTADVIGKLEEKPAVMGHST
                     GGLFAQMLAGRGLSAATVAIDPGVFRGVLPLPFSTLKASGPFLVNPSYRGRAITLTFD
                     QFKYGWANALDEEEAKRLYDTYHVAGSGIALAQMADANLNPWTESKVDTKNPDRGPLL
                     ILDGEKDHTVPWAIANASYKRQKRNPSVTEIKSMPNRGHSLTIDHGWQEVAQTALDFV
                     KRFVPAKPS"
     gene            complement(2066..>2948)
                     /locus_tag="VIH70_08890"
     CDS             complement(2066..>2948)
                     /locus_tag="VIH70_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004940940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8116515.1"
                     /translation="RGVRLGVLILPEHRLPALTEKWSRAEGLGFDHAWTYDHIAWREL
                     RDSPWFAAVPTLAAAAVVTSRIRLGTLVASPTFRHPVPFARELLTLDDLAAGRLTVGI
                     GAGAEGWDTTVLGREPWSMTERLDRFAEFVDLLDRLLTDRAVSFEGRFWSAREARTHP
                     GCIQQPRVPFAIAATGPKSMRVAARHASIWVTNGDRAREGLPLPPDQGAAVVARQLRL
                     FEQACETEGRDPATVDKLVLTGPRLDSGLQSVETFRTVVDAYTAVGITDLVVPWPRHG
                     SPYAGNESILDLIVARS"
BASE COUNT          486 a          995 c          976 g          491 t
ORIGIN      
        1 atgcaggaag accacgtccc ccctccggta ggcatccata gggatcgatc gaactggaac
       61 cactcctccg aacgcctgct gggcgaggcg gtccagatcc cggaacggat cgaagcgcat
      121 gaccactggg tcgtcacccc cctggggcgc gaaggtcgct ctcctattat ccgcaggcgg
      181 accggctctt tcaactcgcg tgcggccacc taattgcggg agcgttcgtt gtgcacgctc
      241 ggccgctcga tcttcgctca gccagagcgt ggtttcctcg agattgccgc ctcgcgtctc
      301 actacgtggc actcccgccc gcttgcttcg ccttgttggt ctggaaggcg tccggcgacc
      361 cgaagtgcgg agaacgatca aatccggtcg ctcctcgcgc tcgaactgtt ggacgagagc
      421 ctgtcaccgg gaccgcccca taccgaagat ctctcaacaa cgcgaacgcc caccgatttg
      481 atcccgccct cgagaaagag gggagggcgg tccttcgacg tggggtggtc tcctacgggg
      541 cttgctgata ctcggcgcgc tgtggtgggc gtggggcgcg tacgcgtggc tgacgaacac
      601 cctcgacccc gaggaaggcg ccgtcaggct cgcggtcttt gccgcgatcg cggcgatgct
      661 cgtgggcagg tgatcgcacg ttcgttggtc cggccgagaa attccggcgg cagatcgaag
      721 ccttagctcg gtttggccgg gacgaaccgc ttgacgaagt cgagcgcggt ctgcgcgacc
      781 tcctgccagc cgtggtcgat ggtcaacgag tggccgcggt tgggcatcga cttgatctcg
      841 gtgacgctcg ggttgcgctt ctgccgcttg taagaggcgt tggcgatcgc ccacggaacc
      901 gtgtggtcct tctccccgtc caggatcagc agcgggcccc gatcgggatt cttcgtgtcg
      961 accttcgact cggtccaggg gttgaggttg gcgtcggcca tctgggccag ggcgatcccc
     1021 gacccggcca cgtggtaggt gtcgtacagg cgcttcgcct cttcctcatc gagggcgttc
     1081 gcccagccgt acttgaactg gtcgaaggtg agcgtgatgg cgcggcctcg atacgagggg
     1141 ttcaccagga aaggtccaga cgccttgagc gtggagaagg gcagcggcag gacaccgcga
     1201 aacacgcccg ggtcgatcgc gaccgtggcc gccgagagcc cgcggccggc gagcatctgg
     1261 gcgaacaatc cgccggtcga gtgccccatc acggcgggct tctcctcgag cttgccgatc
     1321 acgtcggccg tgtggtcggc aacctgcttc agcgtctttt tggcgaggac gtccgggttc
     1381 gcccgagcct cttccaccgt ttccgggtcg tccggccagt ccggcgtcag cggcgcgtaa
     1441 ccggcctgct tgaagaactc cgcccagttc gcccagctgc tcgggagcag ccagaggccg
     1501 tggatgaaga ccaccggagt ggtgccggag gcgttggccg cctcgatctc cctgctctcc
     1561 cgctccgaaa tcgacgccat gccgaactcc tctcggtcgc agtgaaggtg atccgaccct
     1621 agcggaccga cgacgagctc gtccacgagg cgccgacgat cgtggccgtg agacgccgtc
     1681 ggcgctcggc ccgcgacttc ctggagacgc cccgcccaag gtaacggatg gatggatcca
     1741 ccaggcgaga tgcctctcgc ggcctgctgc gtcgagaacg aaaggtggtt gtagcgctcc
     1801 accgccgttt gatcccgtat ctatcccgca cgacgacgaa aacgaggggt agcgaccgga
     1861 atcgaccggg aacggaatcc acaggtcagc cgcccttttc cgccctctac cgcaggtcac
     1921 gaaatcggcc cggatcactc tctcaaggtg gagacacggg ttcgaacccc gttgggacta
     1981 caagcgagaa cggccaggtg aagggccctc ttggcgcgct cagcggcaat gtgggcggcg
     2041 cccacgtgac gccgatgagg acccgtcagc tacgtgcgac gatcagatcg agaatcgact
     2101 cgttccctgc atagggtgac ccgtgacggg gccaaggcac gaccaggtcg gtgatgccaa
     2161 ccgccgtgta cgcgtcgacg acggtgcgga acgtctcgac actttggagc ccgctgtcga
     2221 gacgcggtcc ggtcagtacg agcttgtcca ccgtcgccgg gtcccggccc tcggtctcgc
     2281 aggcctgctc gaacaggcgg agttgccgcg cgacgaccgc cgcgccctgg tccggcggca
     2341 ggggcaggcc ttcccgtgcg cggtcgccgt tggtcaccca gatcgacgca tgtctggccg
     2401 ccacgcgcat cgatttcggc ccggtcgccg cgatcgcgaa gggcacgcga ggctgctgga
     2461 tgcaccccgg atgcgtcctg gcctcccgcg cgctccagaa gcgcccctcg aagctcacgg
     2521 cccgatccgt caggagccgg tcaaggagat cgacgaattc tgcgaacctg tccagccgtt
     2581 cggtcatgct ccacggctcc cgcccgagca cggtggtatc ccagccttca gcaccggcgc
     2641 cgatcccgac ggtgaggcgc ccagcggcga gatcgtccaa ggtgagcagc tcgcgagcga
     2701 acgggaccgg atgccggaag gtgggcgagg cgaccagcgt gccgagacgg atcctggacg
     2761 taaccacggc cgccgccgcg agcgtcggaa ccgcggcgaa ccacggcgag tcccgcagct
     2821 ccctccaggc gatgtggtcg taggtccagg cgtgatcgaa cccgagcccc tcggcccgcg
     2881 accatttctc cgtcagagca ggcaagcggt gctccggcag tatcaggacg cccaagcgca
     2941 cgccccga
//
LOCUS       DATBRK010000293         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2791980, whole genome shotgun sequence.
ACCESSION   DATBRK010000293 DATBRK010000000
VERSION     DATBRK010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791980"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VIH70_08895"
     CDS             complement(<1..467)
                     /locus_tag="VIH70_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116516.1"
                     /translation="MLRLDHVAYAVPDLDEAAVRFREEVGLDSTEGGRHERWGTANRI
                     VPLGDQYLELVAAVDEPTAAETEFGRGVLERAAGGGGWFTIAAVTDDLDAVASRLGLG
                     VGSGSRTRPDGQTVRWRMAGLDDPRREPWMPFFLAWDVPEDLYPGRARAGHGV"
     gene            507..1631
                     /locus_tag="VIH70_08900"
     CDS             507..1631
                     /locus_tag="VIH70_08900"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8116517.1"
                     /translation="MLGRLAPLLVLILAIAPTVARAQEGFEREVDGFGFAVNLALAPD
                     GTMFVADKDRGEIRIVRDGGIVDDPFVTLPVQVTVNETGLLGVAVDPSFPDEPWVYAY
                     YTGTDAVNHLVRIRADGDRGTEIQPLLDLLPATAGWHNGGDLAFGPDGKLYVSVGDGH
                     DGSRSQDPSGIGGRILRLNPDGSIPADNPLGPDNPTFALGIRNSFGLCFDPETGDLWE
                     TENGPTGDDEINLIEPGANYGWPEQLGPGGEPDYVDPVMDFPDTIVPTGCAVSDGVLL
                     FGEGYGGNLHSMPLPGPGEGIVARFDGGITDLESAPDGSIWVVTPTALYRSAGALERP
                     SVTVSPEPEPSSGLVTAGGLLIAGVLIGGLLLMRSRLLRR"
     gene            complement(1628..1792)
                     /locus_tag="VIH70_08905"
     CDS             complement(1628..1792)
                     /locus_tag="VIH70_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004008716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116518.1"
                     /translation="MQKWEYFVAPLLEHNPGEILNTFGDDGWELVSVAQIQNPMGGHS
                     LVAYMKRPKA"
     gene            complement(1792..2235)
                     /locus_tag="VIH70_08910"
     CDS             complement(1792..2235)
                     /locus_tag="VIH70_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011714886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY8116519.1"
                     /translation="MSAETYVPSDPELRSILGDARTIAVVGLSSNPERASYEVAEYLQ
                     SKGYRIVPVNPNETEVLGERAYPTLADIPSEIEIDVVDVFRRAEQTPAIAEEAVARGA
                     KVLWLQEGIANDDARRIGEAGGLSVVMGVCMRKQSQRLEHVEGET"
     gene            complement(2232..2795)
                     /locus_tag="VIH70_08915"
     CDS             complement(2232..2795)
                     /locus_tag="VIH70_08915"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY8116520.1"
                     /translation="MYRSDVRTRLMGSLDPRPSPTAGPGDRLAAVLLPLVDDDRLVLT
                     RRTEHLSRHPGEISFPGGLAHEEDADLAATALRETQEELGLPPEEVEVLGALVPVHTF
                     VSAILIVPFVGVVSGTPTFAPSEDEIAEVLSYTVGDLSAAETTVEWPRDGHVYRGFAY
                     PMSDGNTIWGATAKILNEMIELLREGP"
     gene            2804..>3134
                     /locus_tag="VIH70_08920"
     CDS             2804..>3134
                     /locus_tag="VIH70_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HEY8116521.1"
                     /translation="MVEEAVVSAVERVDRDEVLRFARRLITTPSENPGGTEDAVAAVA
                     VEILEDLGGSPEIVRGEEGRPSVVARFGRGDRPALAWNGHLDVVPAGDPSAWRHPPFG
                     AEVVDGTL"
BASE COUNT          450 a         1147 c         1077 g          460 t
ORIGIN      
        1 cagacgccgt ggccagcccg agcgcgaccg gggtagaggt cctccggcac gtcccacgcg
       61 aggaagaacg gcatccaagg ctcgcgccgg gggtcgtcga gcccggccat ccgccagcga
      121 acggtctggc cgtccgggcg cgtcctcgat cccgagccga ccccgagccc gagccgcgac
      181 gcgacggcgt cgagatcgtc ggtgaccgcg gcgatcgtga accagccgcc gcctcccgcg
      241 gctctttcga gcacgcccct gccgaactcg gtctcggcgg ccgtcggctc gtccaccgcg
      301 gcaacgagct cgaggtactg atcgcccagc ggcacgatcc ggttcgccgt cccccagcgc
      361 tcgtgccgcc caccctcggt cgaatccaat ccgacctcct cgcggaaccg gaccgcggcc
      421 tcatcgagat cggggacggc gtacgcgacg tggtcgagcc ggagcacgcc gccaccctag
      481 tcgaacgacc gacctacact cgggccgtgc tcggcaggct ggcgccgctc ctcgttctca
      541 tcctggcgat cgccccgacg gtcgcgcggg cacaggaggg attcgagcgc gaggtggacg
      601 gcttcggctt cgcggtgaat ctcgctctcg cacccgacgg cacgatgttc gtcgccgaca
      661 aggaccgcgg cgagatccgg atcgtccgcg acggcgggat cgtcgacgac ccattcgtca
      721 cgctaccggt ccaggtgacc gtgaacgaga ccggtctgct cggcgtggcc gtggacccct
      781 cgttccccga cgagccgtgg gtctacgcct actacaccgg gaccgacgcg gtgaaccacc
      841 tcgtgcggat ccgagccgac ggcgaccgcg ggaccgagat ccagcctctg ctcgacctgc
      901 tgccagccac ggcgggctgg cacaacggcg gcgatctcgc gttcggtccc gacggcaagc
      961 tctacgtctc ggtcggggac gggcacgacg ggagccgctc gcaggatccc agcggcatcg
     1021 gcggccgcat cctgcgcctg aaccccgacg gctcgatccc cgcggacaac ccgttgggcc
     1081 ccgacaaccc gacgttcgcg ctcgggatcc ggaactcgtt cggcctgtgc ttcgaccccg
     1141 agacgggtga tctgtgggag acggagaacg ggccgaccgg cgacgacgag atcaacctga
     1201 tcgagccggg cgccaactat ggatggcccg aacagctcgg gccgggcggc gagcccgact
     1261 acgtggatcc ggtcatggac ttcccggaca ccatcgtccc gacggggtgc gccgtctccg
     1321 acggcgtcct gctcttcggc gagggctacg gcggcaacct ccactcgatg ccgctgccgg
     1381 gccccggtga ggggatcgtc gcgaggttcg acggggggat caccgacctc gagagcgccc
     1441 ccgacggctc gatctgggtc gtcacgccga cggcgctgta tcggagcgcc ggcgccctcg
     1501 aacgcccatc ggtcaccgtg tcgcccgaac cggagccgtc gagtgggctc gtgacggccg
     1561 gcgggctcct gatcgccggc gtcctcatcg gcgggttgct gctgatgcgg agccggctgc
     1621 tgcggcgtta ggcctttggg cgtttcatgt aagcgacgag cgaatgaccg cccatcgggt
     1681 tctggatctg cgccaccgag acgagctccc agccgtcgtc gccgaacgtg ttcaggatct
     1741 cgcccgggtt gtgctcgagc agcggcgcga cgaagtactc ccacttctgc atcaggtctc
     1801 cccctccacg tgctccagac gttgcgactg cttcctcatg cacacaccca tgacgaccga
     1861 aaggccgccc gcctcgccga tccggcgcgc gtcgtcgttg gcgatcccct cctgcagcca
     1921 cagcaccttc gcgccgcgcg cgaccgcctc ctcggcgatc gccggcgttt gctcggcgcg
     1981 gcggaacacg tcgacgacgt cgatctcgat ctcgctcggg atgtcggcca gcgtcgggta
     2041 ggcgcgctcg ccgagcacct cggtctcgtt cgggttcacg ggtacgatcc ggtacccctt
     2101 cgactgcagg tattccgcga cctcgtaaga ggcgcgctcg ggattcgacg acagccccac
     2161 gacggcgatc gtgcgcgcgt cgcccaggat cgaacggagc tcgggatcgc tcggcacgta
     2221 ggtctcggcg ctcacggccc ctctcgaagg agctcgatca tctcgttcag gatcttcgcg
     2281 gtcgcgcccc agatcgtgtt gccgtcgctc atggggtacg cgaacccacg gtacacgtgt
     2341 ccgtctctcg gccactccac cgtggtctct gccgccgaga gatcgcccac ggtataggag
     2401 agcacctcgg cgatctcgtc ctcgctcggc gcgaacgtcg gcgtccccga gaccacgccg
     2461 acgaacggca cgatcaggat cgccgagacg aacgtgtgca cgggcacgag cgcgccgagc
     2521 acctcgacct cctcgggcgg cagcccgagc tcctcctgcg tctctcgcag ggccgtcgcg
     2581 gccaggtccg cgtcctcctc atgcgcgagg ccacccggga acgagatctc cccggggtgc
     2641 cgcgagagat gttcggtcct ccgggtgagg acgagccggt cgtcgtcgac caggggcagc
     2701 agcaccgcgg cgagccggtc gcccggaccc gctgtgggcg aggggcgagg atcgaggctg
     2761 cccatcaggc gcgtgcgcac gtcgctccgg tacattcgcc gccatggtag aggaggcggt
     2821 cgtttcggcg gtcgagcggg tggaccggga cgaggtcctg cggttcgcgc gccggctcat
     2881 cacgaccccg agcgagaacc cgggcggcac cgaggacgcc gtggccgcgg tcgcggtgga
     2941 gatcctcgag gacctcggcg gcagccccga gatcgtccgt ggcgaggagg ggcgcccgag
     3001 cgtggtcgcg cggttcggga ggggcgaccg gcccgcgctc gcgtggaacg gccacctcga
     3061 cgtcgtcccc gccggcgacc cctcggcgtg gcggcatccg ccgttcggcg ccgaggtcgt
     3121 cgacggcacg ctgg
//
LOCUS       DATBRK010000294         3599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1923219, whole genome shotgun sequence.
ACCESSION   DATBRK010000294 DATBRK010000000
VERSION     DATBRK010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923219"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..738)
                     /locus_tag="VIH70_08925"
     CDS             complement(259..738)
                     /locus_tag="VIH70_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8116522.1"
                     /translation="MGRALVLNATHQPLAVVAARRAVILVLKEKAEVLVPNGQVFRSE
                     HLHVDAPSVLRLRTFVKIPHRSHAPLTRRAVFARDEWACQYCGANAENLDHVVPRSRG
                     GAHSWDNVVAACRRCNSRKENRLIEEVGLRLARRPVPPRDGFRLSIGRLEPEWEPYL"
     gene            1000..1992
                     /locus_tag="VIH70_08930"
     CDS             1000..1992
                     /locus_tag="VIH70_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8116523.1"
                     /translation="MVREVRLEAREPTLDWRRFAESFRVIEQNVEKVVQGKQPEIRLA
                     LAALVAEGHILIEDVPGVGKTMLAKAIARSIDCSFRRIQFTPDLLPTDVTGVNVFNQE
                     RGDFEFKPGAIFANIVLGDEINRASPKTQSALLECMEERQITVDTETYQLGTPFMVIA
                     TQNPIEHEGTYPLPEAQLDRFMMRIGIGYPSSEVEAEILATHGVRSTLPDIGPVADAL
                     SVREMIEQTRQVHVSPAVRRYIVDLVEGTRRHPDVYLGASPRASIMLLRASRAIAAAE
                     ERDYVIPDDVKSLVTPVLAHRVIVTADAVMGGRSSPAVLQDLAAEVAVPVTGNA"
     gene            1994..3226
                     /locus_tag="VIH70_08935"
     CDS             1994..3226
                     /locus_tag="VIH70_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8116524.1"
                     /translation="MPSGRGVLVFGAGIALWVAARMLGSPTLHMISVGIVLLPFAAAL
                     FARWSRQRLRIRRRLSDTRVQPGQRVHIELEIENQSPAQTSFLLVEDHLPAQLGRSAR
                     LVVASLAPRGRQRVRYSIAPHQRGRYVLGPLTADLSDPFALTKLRIEFDERDELVVAP
                     EVELLGSGPDSPFGMTSGLALAKHLFRTGDEFYTMRAYVEGDDLRRIHWPSVARSGEL
                     MIRQDESTRRSTAVLFVDTRDTAVGQTHTPCFEKVISAAASLGILLMRYGFSVKLATT
                     QMPPQRVAEESLLDALAGMTHHSSRALSTGLTRLRMVAASDTTLVVVGAPPGPTELPS
                     WLRVGAMFGPKVGVFVHTTEPDTLPPDRRAQLEGRASQARMSMSRTGWEVVVLPPSRR
                     LRDVWHVERTQPLVTSGS"
     gene            3253..>3599
                     /locus_tag="VIH70_08940"
     CDS             3253..>3599
                     /locus_tag="VIH70_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116525.1"
                     /translation="MAMALAFGRVFTGRQATLELVAAALASVAIAAMFERRGLLLATI
                     ASLLGLAFAITWIVLPQTAWYDLPSIQTLRAVGRSLEFVGQQARVRVAPTPALPPLML
                     AAVTAVWTAAFAS"
BASE COUNT          537 a         1256 c         1248 g          558 t
ORIGIN      
        1 tcggcgaata cctcacggaa ccgaccgggc aggctgatgc gccccttcgg gtcgagctga
       61 tagctgtgcg tgccgagcag ttcggccacg cgtcctcccc ttctcctcca ccgcgatcca
      121 catttattcc actgtcactc caccccgtca agggttcaac gtgaaatggg ttaacgacag
      181 tgtccgtgcg tcaaccgttg agacgaagga cgagccggtg agtagtgagt ttgtgagcgg
      241 tgagcggtga gcggcgagct agaggtaggg ctcccactca ggttcgaggc ggccgatcga
      301 caggcggaac ccgtccctcg gcgggaccgg tcggcgcgcg aggcggagtc cgacctcctc
      361 gattagccgg ttctccttgc gggagttgca tcggcggcac gccgcgacca cgttgtccca
      421 cgaatgggcg cctccgcgcg acctcgggac gacgtggtcc aggttctccg cgttggctcc
      481 gcagtactga cacgcccact cgtcgcgagc gaagaccgcg cggcgagtga gcggcgcgtg
      541 ggaccggtgg gggatcttga cgaaggtccg cagacgcagg accgagggcg cgtcgacgtg
      601 caggtgctcg gatcggaaga cctgtccgtt gggaaccagg acctcggcct tctccttcag
      661 caccaggatc acggcccgtc tcgcggccac caccgcgagc ggctggtgcg tcgcgttcag
      721 cacgagcgct cgtcccacgg cgcgagtata cgggccacca ggcacagaac cgggcgcgag
      781 gtgcttgcaa cgccggaatc gcagggttgt aatccgcgcg gaggcggcgg gggccgcgct
      841 cgaaggacgc ctggaagagc gaacaagggg ccgcgcgggg cgtccggcag ctggcgtgcc
      901 gctgccgcct ccactcctcc gatcggaacg cggtgcatcg tcggcctgag caggatcccg
      961 caggaacccc gacctattct tgaggtactc ctgagtaaga tggtccggga ggtgcggttg
     1021 gaggctcgag aacccacgct cgactggcgg cgattcgcgg agagcttccg cgtcatcgag
     1081 cagaacgtgg agaaggtggt ccagggcaag cagccggaga tccggctggc gctcgccgca
     1141 ctcgttgccg agggtcacat cctgatcgag gacgtccccg gtgtcggcaa gacgatgctc
     1201 gccaaggcga tcgcgcgctc gatcgactgc tcgttccgac ggatccagtt cacgcccgac
     1261 ctgctgccga ccgacgtcac cggcgtcaac gtgttcaacc aggagcgcgg ggacttcgag
     1321 ttcaagcccg gtgcgatctt cgcgaacatc gtgctcgggg acgagatcaa tcgcgcgagc
     1381 cccaagaccc agtcggcgct cctggagtgc atggaggagc gccagatcac ggtagacacc
     1441 gagacctatc agctcggcac gccattcatg gtgatcgcga cgcagaaccc gatcgagcac
     1501 gaggggacct atccgctgcc cgaggcgcag ctcgaccggt tcatgatgcg gatcgggatc
     1561 ggctacccct ccagcgaggt cgaggccgag atcctcgcca cgcacggcgt gcgttcgacg
     1621 ctgccggaca tcggaccggt cgccgacgcc ctgtcggtac gcgagatgat cgaacagacc
     1681 cggcaggtcc acgtctcacc cgccgtgcgc cgctacatcg tcgatctggt cgaggggact
     1741 cgccgtcacc cggacgtgta cctgggcgcg agccccaggg cgtcgatcat gctgctccgc
     1801 gcctcgcgtg cgatcgcggc cgccgaggag cgcgactacg tgatcccgga cgacgtgaag
     1861 tcgctcgtga cgccggtgct cgcgcaccgc gtgatcgtga cggccgacgc cgtcatgggc
     1921 ggacgcagct cgccggccgt gctgcaagac ctcgccgccg aggtggcggt acccgtcacc
     1981 gggaacgcct gacatgccct cgggccgggg ggtcctggtc ttcggggccg ggatcgcgct
     2041 ctgggtggct gcacggatgc tcgggtcgcc gaccctgcac atgatttcgg tcgggatcgt
     2101 cctgctcccg ttcgccgccg ccctgttcgc ccggtggagc cggcagcggc tgcgcatccg
     2161 gcgacggctc tcggacacgc gcgtgcaacc cggccagcgc gtgcacatcg agctcgagat
     2221 cgagaaccag tccccggccc agacctcctt cctcttggtg gaggaccacc tccccgcgca
     2281 gctcggccgg tcggcgcggc tggtggtcgc ctcgctggcc ccccgcggga ggcaacgggt
     2341 ccgctactcc atcgccccgc accagcgggg gcgctacgtc ctcgggccgc tcaccgcgga
     2401 cctgtccgat ccgttcgcgc tcacgaagct acggatcgag ttcgacgagc gtgacgagct
     2461 cgtcgtcgcg ccggaggtcg agctgctcgg gagcggaccg gactcaccgt tcggcatgac
     2521 gagcgggctc gcgctggcca agcacctgtt ccgcaccggc gacgagttct acacgatgcg
     2581 ggcctacgtg gagggcgacg acctgcgacg gatccactgg ccgagcgttg cccgcagcgg
     2641 cgagctgatg atccgccagg acgaatccac ccggcgatcg accgccgtcc tgttcgtcga
     2701 cacgcgggac accgccgtgg gtcagaccca tacgccctgc ttcgagaagg tgatctcggc
     2761 ggcggcctcg ctcgggatcc tgctgatgcg ctacgggttc tccgtcaagc tcgccaccac
     2821 gcagatgcca ccacagcgcg tcgcggagga gagcctgctc gacgcgctcg cggggatgac
     2881 ccaccactcg tctcgagcgc tgtcgaccgg actcacgcgc ctgcgcatgg tcgccgcgag
     2941 cgacacgacc ctggtcgtgg tcggggcgcc tcccggcccg accgagctgc cctcgtggct
     3001 gcgcgtcggc gcgatgttcg gccccaaggt cggggtgttc gtccacacga ccgagcccga
     3061 caccctgccc cccgaccgcc gggctcagct cgaggggcgc gcgtcacagg cccggatgtc
     3121 gatgtcccgg accgggtggg aggtcgtggt gctcccaccg tcgaggagat tgcgagacgt
     3181 atggcacgtc gagcggaccc aacccctggt caccagcggc tcctagggct cgcggccgtc
     3241 gcggctctgt ccgtggcgat ggcgctcgcg ttcggccgcg tgttcacggg ccggcaggcc
     3301 acgctcgagc tcgtggccgc ggcgctcgcg tcggtggcga tcgcggccat gttcgaacgc
     3361 cgggggctgc tgctggcgac gatcgcgagc ctgctcggcc tcgcgttcgc gatcacctgg
     3421 atcgtcctgc cgcagacggc ctggtacgac ctcccgtcga tccagacgct ccgggccgtg
     3481 gggcgctcgc tcgagttcgt cgggcagcag gcgcgcgtgc gcgtggcgcc cacgcccgcg
     3541 ctccctccgc tgatgctcgc ggcggtgacg gccgtatgga ccgcggcctt cgcgtcgca
//
LOCUS       DATBRK010000295         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1923302, whole genome shotgun sequence.
ACCESSION   DATBRK010000295 DATBRK010000000
VERSION     DATBRK010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923302"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1453
                     /locus_tag="VIH70_08945"
     CDS             95..1453
                     /locus_tag="VIH70_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthetase"
                     /protein_id="HEY8116526.1"
                     /translation="MKVVVLVDGEHYPPVTRWAIDELRGRGLDPIAAVFAGGGEKLDP
                     SGALDLGIPLRGSVPDRSVASALAVAIDELAPEGIFDLSDEPVLGYRERMEVAAVALA
                     RGVAYLGPDFRLDPPVDQGPLPVATIAVIGIGKRTGKTAVSGETARVAAAHGFDPVVV
                     AMGRGGPPEPEIAEAGSVTLEALLELVRRGRHAASDYLEIAATSGVTTVGARRAGGGL
                     AGRSSSTNVRAAAEVALGLGARTVIVDGSGASMPPFPWDAGILVVPATAPPEYLGGYL
                     GPLRLLLSDLVVVTMARSPAGLQNIPTLRSHAERLNADARLIVTDFEPQPLGDVRGRD
                     VFFTTTAPGAVAARQAEALERTHGCRVVGWSARLADRAGLARDLDGAGAYEVLLSELK
                     AAAVDVACDRAMARGAEVVFVDNRAVELEGDTDLPTALRETIGLAGERSAQRNDQRSQ
                     QR"
     gene            1450..2439
                     /locus_tag="VIH70_08950"
     CDS             1450..2439
                     /locus_tag="VIH70_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116527.1"
                     /translation="MTNDPKNPQILISDRDSGLPYSKGLMASQVMVTGLSPVRAYQVA
                     EAIEQRLRELGVPSISSEQLGELALGVLVDLAGERYARNFRRWREIEALQVPLVVLIG
                     GATGVGKSTLATQLATRLGIVRVVATDAIREVMRALFSHELMPTLHASSFEAGSVLRE
                     PPSRDEVVVGFREQTAAVAVGVQALVERAAMEGTHLIIEGAHVVPGFLDLDPWRERIL
                     AVPVVITIEEDEVHRSHFAARASEHTGRPAERYLDRFDDIRRVQRYIKSQALSHGVPV
                     IANYSFDRALAAIIDLVMERATQRTVPAGVTAGGERGGRAPAPVTAANEGGRT"
     gene            2436..3098
                     /gene="fsa"
                     /locus_tag="VIH70_08955"
     CDS             2436..3098
                     /gene="fsa"
                     /locus_tag="VIH70_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEY8116528.1"
                     /translation="MKLFLDTANIEEIREINRWGVLGGVTTNPSLVAKENDDPERVWK
                     EILSEVDGPISLETTELDPEAMYRQGLDLAQMAPNAVVKVPMTPGGLNAGKRLIDDGI
                     KINVTLVFSPAQAILAAEIGAYIVSPFLGRLDDVASDGMDALRKICDIYEIQGYDTLV
                     LAASLRHPMHIVESALAGADIATMPFAVFKQLVKHPLTDAGLEKFLGDWKALKQELEK
                     GA"
BASE COUNT          510 a         1078 c         1193 g          463 t
ORIGIN      
        1 gagctcgagc cgccggtgca gcagcgccgc ctccccgcgg ctcaccaggg ggagtcctag
       61 ctccgaaccg caccgccccg aaggagccca tcggatgaag gtcgtcgtcc tcgtcgacgg
      121 tgagcactac ccgcccgtca cccgctgggc gatcgacgag cttcgcgggc gcgggctcga
      181 tccgatcgcg gcggtgttcg cgggtggcgg cgagaagctc gacccgtcgg gcgcgctcga
      241 cctcgggatc ccgctgcgcg gttcggtccc ggaccgttcg gtcgcatcgg cgctcgccgt
      301 cgcgatcgac gagctcgccc ccgaggggat cttcgacctg tcggacgaac ccgtgctcgg
      361 gtaccgagag cggatggagg tcgcggccgt cgcgctggca aggggcgtgg cctacctcgg
      421 tcccgacttc cggctcgacc cgcccgtcga ccaggggccg ctgcccgtcg cgacgatcgc
      481 ggtgatcggg atcgggaagc gaacgggcaa gaccgccgtt tcgggcgaga ccgcgcgcgt
      541 ggccgcggca cacgggttcg atccggtcgt cgtcgcgatg ggcaggggag ggcctcccga
      601 acccgagatc gccgaggcgg gctcggtcac gctcgaggcg ctgctcgagc tggtccgccg
      661 gggccgccat gccgcctcgg actacctgga gatcgcggcc acgagcggcg tcacgacggt
      721 gggcgcccgg cgggcgggcg gcgggcttgc ggggcgctcc tcgtcgacca acgtccgcgc
      781 ggccgcggag gtcgcgctcg ggctcggcgc ccggaccgtc atcgtcgacg ggagcggggc
      841 ctcgatgccc ccgttcccgt gggacgcggg gatcctggtc gtcccggcca ccgctccgcc
      901 ggagtacctc ggcgggtacc tggggcccct gcggctgttg ctatcggatc ttgtcgttgt
      961 taccatggcg cgtagcccag ccgggctgca gaacatcccc accctcaggt cccacgccga
     1021 acggctgaac gctgatgccc ggctcatcgt gacggacttc gaaccacagc cactcggcga
     1081 tgtgcggggg agggacgtgt tcttcaccac gacggcgccc ggggcggtgg ccgcgagaca
     1141 agcggaagcc ctcgagcgga cccacggatg ccgagtggtc gggtggagcg ccaggctcgc
     1201 cgatcgggcc ggcctggcac gagacctgga cggggcgggg gcctacgagg tgctcctgtc
     1261 ggagttgaag gccgccgcgg tcgacgtcgc gtgcgaccgg gcgatggccc ggggcgccga
     1321 ggtggtcttc gtggacaacc gcgctgtgga gttggagggc gacacggacc ttccgaccgc
     1381 attgagggag acgatcggtc tcgccggaga gcgaagcgcc caaagaaacg accagagaag
     1441 ccaacagaga tgacgaacga ccccaagaac cctcagatcc tgatctccga tcgcgactcg
     1501 ggcctgccgt actcgaaggg cctgatggcg tcgcaggtca tggtgaccgg gctgtcgccg
     1561 gttcgcgcgt accaggtcgc cgaggcgatc gagcagcgcc tgcgcgagct cggtgtcccg
     1621 tcgatctcga gcgaacagct cggggagctc gcgctcggcg tcctcgtgga cctggccggc
     1681 gagcgatacg cgcggaactt ccggcgctgg cgcgagatcg aggcgctcca ggtccccctg
     1741 gtggtgctga tcggtggggc gaccggggtg ggcaagtcca cccttgcgac ccagctcgcg
     1801 acgcggctcg ggatcgtccg agtcgtcgcg accgacgcga tccgagaggt gatgcgcgcg
     1861 ctgttctccc acgagctcat gccgacgctg catgcgtcgt cgttcgaggc gggctcggtc
     1921 ctacgtgagc ccccgtcgag ggacgaggtg gtcgtcgggt tccgggagca aaccgcggcg
     1981 gtcgcggtcg gtgtgcaggc gctcgttgag cgcgccgcga tggaggggac gcacctgatc
     2041 atcgagggcg cccacgtggt gccgggcttc ctggacctcg acccttggcg cgaacgcatc
     2101 ctggccgtcc cggtcgtgat cacgatcgag gaggacgagg tgcaccgaag ccacttcgcg
     2161 gcgcgggcat cggagcacac agggcggccg gcggagcgct acctcgaccg gttcgacgac
     2221 atccggcgcg tccagcggta catcaagagc caggcgctgt cgcacggcgt ccccgtgatc
     2281 gccaactaca gcttcgatcg cgccctggcc gcgatcatcg acctcgtcat ggaacgagcg
     2341 acccaacgca cggtgccggc cggggtcacc gcgggcggcg agcgcggggg tcgtgcgccg
     2401 gctcccgtga cggcggcgaa cgaaggaggc agaacgtgaa gctgttcctg gacacggcca
     2461 acatcgagga gatccgcgag atcaaccgct ggggcgtcct cggcggcgtg accaccaacc
     2521 cctcgttggt ggcgaaggag aacgatgacc ccgagcgggt ctggaaggag atcctgtcgg
     2581 aggtcgacgg accgatctcg ctcgagacga ccgagctcga tcccgaggcg atgtaccggc
     2641 aggggttgga tctggcacag atggcgccga acgccgtcgt gaaggtcccg atgacccccg
     2701 ggggcctgaa cgcgggcaag cgcctgatcg acgacgggat caagatcaac gtcacgctcg
     2761 tgttctctcc ggctcaggcg atcctcgccg ccgagatcgg ggcgtacatc gtgtcgccgt
     2821 tcctcgggcg cctggacgac gtcgcgtccg acgggatgga cgcgctccgc aagatctgtg
     2881 acatctacga gatccagggg tacgacacgc tggtgctcgc cgcgtcgctg cgacacccga
     2941 tgcacatcgt cgagtcggcg ctcgcgggcg ccgacatcgc gacgatgccc ttcgcggtgt
     3001 tcaagcagtt ggtgaagcac ccgctcaccg acgcaggact ggagaagttc ctcggggact
     3061 ggaaggccct caagcaggaa ctcgagaaag gagcatgagt ggccaccacc gataagcgtg
     3121 tcctggaggg aaaggtcgcc gcggagctgc atcagatcgc ggcggacctg ggtatcgagg
     3181 ggcacaaggg attgaagaag gccgacctgg tcgacaggat catcgcgcac accaacggcg
     3241 agcg
//
LOCUS       DATBRK010000296         4262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2792569, whole genome shotgun sequence.
ACCESSION   DATBRK010000296 DATBRK010000000
VERSION     DATBRK010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2792569"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VIH70_08960"
     CDS             complement(<1..221)
                     /locus_tag="VIH70_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116529.1"
                     /translation="MAEVSNDDATLFCEVEGEGEPVTVFAHGLTNNRNELAAFTPLVP
                     GTKVRFDFRGHGRSSSPADPKAFRFEDFAR"
     gene            262..537
                     /locus_tag="VIH70_08965"
     CDS             262..537
                     /locus_tag="VIH70_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116530.1"
                     /translation="MADVTVKRRADGDPMWFDVVVREGDATTEHAVSVSEADLDRLGA
                     GRPPEAFVRDSFAFLLEREPKESILRSFDLSAIGRYFPEFEDEIAER"
     gene            complement(553..1089)
                     /locus_tag="VIH70_08970"
     CDS             complement(553..1089)
                     /locus_tag="VIH70_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8116531.1"
                     /translation="MEMERLPTPDPVAEAAEYQRYLTGLVGDDDPAEVQAGSAEAWRE
                     VIRTAGARVGERPEPDEWSVLECLGHVTDSELVVASRYRWIVAQDEPPLVGYDQDLWV
                     DRLHHNEDDPEVLLETFEALRRANLALWARSTEQERARVGLHSERGRESFDLTFNLAA
                     GHDRFHLDQARRALAALG"
     gene            complement(1097..>4262)
                     /locus_tag="VIH70_08975"
     CDS             complement(1097..>4262)
                     /locus_tag="VIH70_08975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8116532.1"
                     /translation="PGLIAGDWGPFGMFGGGYRVSVPLMGSILQRVAGIDLYTFSAFL
                     MVGVPVLTGLALGAFSWRTHRDPLLFLLVMLSTAALSMTTPYVGYLDNITVLYFLSLI
                     LAFFAPARTSWGARSAMFLVGIAAAFTHPTTCVIFGFSLMAVFGLRLLTSRFHLGPPL
                     KELGPSLMSVGFGMIFGLATWLIAPWGVTGSLADAALPPPYTKEVFLDRLKGWIGSLQ
                     PGILIPLIVLAIAWTLWTARRNRERSDEFATISAMLLLPFLGIFGFVAATYPYYRFMN
                     ATMALFALGGLGAYVAIRWLWRREGAAKVAGVAASLLIVASFGFIWTQGREISHWADP
                     DSQWIDQPTRTALEAARAVVENEPADRPIVFVVNYQDTYQSYGWSKTFTNVSRTGLPG
                     DAVKRSMTYFGSVEDFLADQPTVLTNDTYNKMSRAFQRELTDLREEYTGPPIAILVRQ
                     FNEGTVNEELLDQAPDDLVILGDDVAVVTGEGLTTPSDAAIADARAAEGEVGAFYADH
                     PGVLGNLGHTLWVVFALALLLVVPGAIAARWFGLDDAWLKIALVPGISITLTVLSGIV
                     VVAVTRAPFGAVHGWASLALATAVAAGLRFGASPLLRVLNGFSGFFDSMFSVFSNRDF
                     SVLVGMQYLAQMGQGVVQGAIGKSIAFGGEKGFDVSTVPSADYLLKVVLALYVPYTLI
                     SPFIGVFIDRFERRKVVEWTNVVVAAVVVVVAVVAMIPLGKATTEGDVGATVALVIGL
                     LAAQACVRVALAVKSAAMPDVLSGKDLLQGNGLSQAGGALFQVVGIGFALGAAAALPA
                     WLVVVVGAGLLGVAALVARHMRHAEASVHETSFGAEASQVLRNIAAGIRELAARPPAA
                     VGLSSFQMLRYQFWGFVLFTFALYAKNLVQGGDASNVALGLSGGGGLIGGALGLILAQ
                     KWKDRVPPIRLLLASMLLLGAGTVVFGAPVSLAGFTALLFVGFFSFFLGKISADTIVQ
                     QAMPDDFRGRAFALFDIAYNLGFIVPALILSFVWVENDPSQVRTILVVSGAVFLVLTA
                     LVWRWSRSIRDEFAPQDDLVEQEV"
BASE COUNT          742 a         1534 c         1434 g          552 t
ORIGIN      
        1 cgcgcgaagt cctcgaaccg gaacgccttg gggtccgcgg gcgagctcga acggccgtgc
       61 cctcggaagt cgaacctcac cttggtcccg ggcaccagcg gcgtgaaggc ggcgagctcg
      121 ttgcggttgt tggtcagccc gtgcgcgaag acggtgacgg gctcgccctc gccctccacc
      181 tcgcagaaca gggtcgcgtc gtcgttgctg acctcggcca cgagccaaac ctaccgaagc
      241 cccggtcgta cgatgcgccg gatggccgac gtcaccgtga agcgcagggc ggacggggac
      301 ccgatgtggt tcgacgtggt cgtgcgcgag ggcgacgcta ccaccgagca cgccgtttcc
      361 gtgtccgagg cggacctcga tcggctcgga gctgggcgac cgcccgaggc gttcgtgcgc
      421 gactccttcg cgttcctgct cgagcgcgaa ccgaaggagt cgatcctgcg ctccttcgac
      481 ctgagcgcga tcgggcgcta cttccccgag ttcgaggacg agatcgccga gcggtagggc
      541 ctccgctcga tctcagccga gcgcggcgag cgcccggcgg gcctggtcca ggtggaaccg
      601 gtcgtggccg gccgcgaggt tgaacgtcag atcgaagctc tcgcgtccgc gctcgctgtg
      661 cagtccgacc cgcgcccgct cctgctcggt ggagcgggcc cacagcgcga ggttcgcgcg
      721 gcggagggcc tcgaacgtct cgagcaacac ctccgggtcg tcctcgttgt ggtggagccg
      781 gtcgacccac aggtcctggt cgtagccgac gagcggcggc tcgtcctgcg cgacgatcca
      841 gcggtaccgg ctcgcgacga cgagctcgga gtccgtgacg tgacccaggc actcgagcac
      901 ggaccactcg tcgggctcgg ggcgttcgcc gacgcgggcg cccgcggtcc ggatcacctc
      961 gcgccaggcc tcggcggatc ccgcctggac ctcggccggg tcgtcgtctc cgacgagacc
     1021 ggtgaggtag cgctgatact cggcggcctc ggccaccgga tcgggcgtgg gcagacgctc
     1081 catctccatc tgttggctac acctcctgtt cgacgaggtc gtcctgcggg gcgaactcgt
     1141 ctcggatcga tcgcgaccag cgccacacga gcgcagtcag cacgaggaac acggcgcccg
     1201 agacgacaag gatcgtgcga acctgcgacg ggtcgttctc gacccacacg aacgagagga
     1261 tcagcgccgg cacgatgaac ccgaggttgt acgcgatgtc gaacagcgcg aacgcccgcc
     1321 cccggaagtc gtccggcatc gcctgttgga cgatcgtgtc cgccgagatc ttgcccagga
     1381 agaacgagaa gaacccgacg aacagcaggg cggtgaaccc ggccagcgac accggcgcgc
     1441 cgaatacgac ggtcccggcg ccgagcagca gcatcgaggc gagcagcagg cggatcggcg
     1501 ggacgcggtc cttccatttc tgcgcgagga tcaggcccag cgcgccgccg atcagcccgc
     1561 ccccacccga caggccgagc gcgacgttgc tcgcgtcgcc gccctgcacg aggttcttcg
     1621 cgtacagcgc gaacgtgaac agcacgaacc cccagaactg gtagcggagc atctggaacg
     1681 acgacaggcc gaccgcggcc ggtgggcgag ccgcgagctc gcggatcccg gccgcgatgt
     1741 tccgcagcac ctgggaagcc tccgcgccga agctcgtctc gtgcaccgag gcctcggcgt
     1801 gccgcatgtg cctcgcgacc agggcggcga ccccgagcag ccccgcgccg acgaccacga
     1861 cgagccacgc gggcagcgcg gccgccgcgc ccagcgcgaa cccgatcccc accacctgga
     1921 acaacgcccc acccgcctgc gacaggccgt tgccctggag caggtcctta cccgacagga
     1981 cgtcgggcat cgccgcagac ttcaccgcga gcgcgactcg cacgcacgcc tgggcggcga
     2041 gcaggccgat cacgagcgcg acggtggcgc cgacatcgcc ttcggtggtc gccttgccga
     2101 gcgggatcat cgcgaccacg gccaccacga cgacgaccgc ggcgacgacg acgttggtcc
     2161 attcgaccac cttgcggcgc tcgaaccggt cgatgaacac gccgatgaac ggcgagatca
     2221 gcgtgtaggg gacgtagagc gcgagcacca ccttcaacag gtagtccgcg gacggaaccg
     2281 tcgagacgtc gaaccccttc tcgcccccga acgcgatcga cttgccgatc gcgccctgca
     2341 cgacgccctg ccccatctgc gccaggtact gcatgccgac caagacggag aagtcgcggt
     2401 tggagaagac cgagaacatc gagtcgaaga agccgctgaa gccgttcagc acacgcagca
     2461 gcggtgacgc tccgaaccgc aacccggccg cgaccgcggt cgcgagcgcc agcgaggccc
     2521 agccgtggac ggcgccgaac ggcgctcggg tgaccgccac cacgacgatg cccgagagca
     2581 ccgtgagcgt gatcgagatc cccggcacga gcgcgatctt cagccacgcg tcgtcgagcc
     2641 cgaaccaccg cgccgcgatc gctcccggca cgacgagcag cagggcgagc gcgaacacca
     2701 cccacagcgt gtgcccgagg ttcccgagta cccccgggtg atccgcgtag aaggcaccga
     2761 cctccccttc cgccgccctc gcgtccgcga tcgccgcgtc cgagggcgta gtgaggccct
     2821 cgcccgtgac gaccgcgacg tcgtcgccga ggatcacgag gtcgtccggc gcctggtcca
     2881 gcagctcctc gttcacggtc ccctcgttga actggcggac caggatcgcg atcggcgggc
     2941 ccgtgtactc ctcgcgcagg tcggtgagct cgcgctggaa cgcgcgtgac atcttgttgt
     3001 atgtgtcgtt cgtgagcacg gtcggctggt cggccaggaa gtcctcgacg ctgccgaagt
     3061 acgtcatcga gcgcttcacg gcgtcgcccg gcaggccggt gcgcgagacg ttcgtgaacg
     3121 tcttggacca accgtaggac tggtacgtgt cctggtagtt cacgacgaac acgatcgggc
     3181 ggtcggcggg ctcgttctcg acgacggccc gcgccgcctc gagcgccgtc cgcgtcggtt
     3241 ggtcgatcca ctggctgtcg gggtcggccc agtgcgagat ctcgcggccc tgcgtccaga
     3301 tgaaaccgaa cgacgccacg atcaggagcg acgccgcaac gccggcgacc ttggccgcgc
     3361 cctcacgcct ccagagccac cggatcgcga cgtacgcgcc gagcccgccc agcgcgaaca
     3421 gcgccatcgt ggcgttcatg aagcggtagt acgggtaggt ggccgcgacg aacccgaaga
     3481 tgccgaggaa cggcagcagc agcatcgccg agatcgtggc gaactcgtcc gagcgctccc
     3541 ggttcctgcg cgccgtccag agcgtccacg cgatcgccag cacgatcagc gggatcagga
     3601 tccccggctg cagcgaaccg atccacccct tcagccggtc gaggaagacc tccttcgtgt
     3661 acgggggcgg cagggctgcg tccgcgagtg agccggtcac gccccacggc gcgatcagcc
     3721 acgtcgccag cccgaagatc atcccgaacc cgaccgacat cagcgacggt ccgagctcct
     3781 tcagcggcgg acccaggtgg aaccggctcg tcagcagccg cagcccgaac accgccatca
     3841 gcgagaagcc gaagatcacg cacgtcgtcg ggtgcgtgaa cgccgccgcg atcccgacca
     3901 ggaacatcgc gctccgcgcg ccccacgagg tcctcgcagg cgcgaagaac gcgaggatca
     3961 acgagaggaa gtacaggacc gtgatgttgt cgaggtagcc gacgtacggc gtggtcatgg
     4021 acagcgccgc cgtcgagagc atgaccaaca ggaacagcag ggggtccctg tgcgtccgcc
     4081 acgagaaggc gcccagcgcg aggcccgtca ggacggggac gccgaccatc aggaacgcgc
     4141 tgaacgtgta caggtcgatc ccggcgaccc gctgcaggat cgagcccatc agcggcacgc
     4201 tcacccggta cccgccgccg aacatcccga acgggcccca gtccccggcg atcagaccgg
     4261 gg
//
LOCUS       DATBRK010000297         1923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1349212, whole genome shotgun sequence.
ACCESSION   DATBRK010000297 DATBRK010000000
VERSION     DATBRK010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349212"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1923)
                     /locus_tag="VIH70_08980"
     CDS             complement(<1..>1923)
                     /locus_tag="VIH70_08980"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEY8116533.1"
                     /translation="TGTIGLLMDCDTTGIEPDLALVKTKKLVGGGSMSIVNQTVPRAL
                     RTLGYADEHVQAITEYVAEHNSVKGAPALRKEHYEVFDCAMGDSPIHYMGHIRMMAAA
                     QPFISGAISKCVTGETLVATSDGLVRIGSLYEGEAADTFRDKILAVASLDGLQKTDAF
                     YYGGVRPVRRATLRSGHTVTGTPNHRLMVATKQGLQWRTLAELEVGEYVAQQYGSDLW
                     SALPARFDDFAPTARYGNQKTVRIPTEMTEELAFLLGAFAAEGHIARPTHTVVITNSV
                     ESVLERVVAAWRGQFGVEARITRETGRCPGVVVNSKAIVEFLDHLGCGGRASEKRIPD
                     AVLRSPRPMVLAFLRGLALDAYVTTTGMVKWAICLDAPKLLDDLQAVLTNLGVVHGRI
                     TKFNPKYGKSYDEVYASGEQARRLVELVPFCEPEKAARAARLAAMSFAQSTADVIPGI
                     TGPELYNAIPKGKSGRNGWSLRRGLTFLLDPRTKQVSRRTLERVAAIPGVELPEWLRA
                     VLRDNLHFSPVSSVEDAGRREVFDLSVPVQYAFVGNGIVNHNTVNMPEHVTVEEVEHA
                     YVEGWKLGLKALAIYRDNCKVGQPLSADRKVTTDTGAVIELDHAPVRRRLPKQRPSKT
                     TSFRVGDIEGYLTAGS"
BASE COUNT          306 a          670 c          613 g          334 t
ORIGIN      
        1 taggacccgg cggtcaggta gccctcgatg tccccgaccc ggaacgaggt ggtcttggac
       61 ggccgctgct tgggcaggcg ccgccgcacc ggggcgtggt cgagctcgat gaccgcgccg
      121 gtgtcggtgg tcaccttgcg gtcggccgac agcggctgcc cgaccttgca gttgtcccgg
      181 tagatcgcca gcgccttgag cccgagcttc cacccttcga cgtaggcgtg ctcgacctcc
      241 tcgacggtga cgtgctcggg catgttcacg gtgttgtggt tgacaatccc gttgccgacg
      301 aacgcgtact gaaccggcac cgagaggtcg aacacctcac gccggcccgc gtcctccacg
      361 ctcgacaccg ggctgaagtg gaggttgtcc cgcagcaccg cacgaagcca ctcgggcagc
      421 tcgacaccgg ggattgccgc cacccgctcg agggtgcgac ggctcacctg cttggtgcga
      481 gggtcgagca ggaacgttag cccgcgccgc aggctccagc cgttccgacc cgacttgccc
      541 ttcggaatgg cgttgtagag ctcgggtcca gtgatgcccg ggatgacgtc ggcggtactc
      601 tgcgcgaagg acatcgcggc cagcctcgcg gcccgcgccg ccttctccgg ttcgcagaag
      661 ggcacgagct cgacgagccg gcgcgcctgc tccccggacg cgtagacctc gtcgtaggac
      721 ttaccgtact tcggattgaa cttggtgatg cgaccgtgca ccacccccag gtttgttagg
      781 accgcctgga gatcgtccag cagcttcggc gcgtccaggc agatggccca cttgaccatc
      841 ccggtggtgg tcacgtacgc atcgagcgcc aggccccgca ggaacgcgag caccatcggt
      901 cgcggtgacc gcagaaccgc gtcgggaatg cgcttctcgc tcgcgcgccc tccgcagccg
      961 aggtggtcga ggaactcgac gatggccttg gagttaacga cgacaccggg gcacctcccg
     1021 gtctcacggg tgatgcgggc ctccacgccg aactgacccc gccacgccgc aacgacccgc
     1081 tccaacaccg actcgaccga gttggtgatc accacggtgt gggtcggccg ggcaatgtgg
     1141 ccctctgccg cgaaggcgcc gagtaggaag gccagctctt cggtcatctc ggtcgggata
     1201 cggacggtct tctggttccc gtacctggcg gtgggcgcga agtcgtcgaa cctggccggt
     1261 agcgccgacc acagatcgct tccgtactgc tgggcgacgt actcgccgac ctcgagctcg
     1321 gccagcgtgc gccactgcag tccctgcttc gtcgcgacca tcagccggtg gttcggggtc
     1381 ccggtcaccg tgtgccccga gcggagcgtc gcccgccgga ccggacgcac cccgccgtag
     1441 tagaacgcat cggtcttctg gagaccgtcc aacgacgcga cagcgaggat cttgtcccgg
     1501 aatgtgtccg cagcctcgcc ttcgtacagg ctcccgatgc gaacgagccc gtcgctggtt
     1561 gccaccagcg tctcgcctgt cacgcacttg gagatggccc cactaatgaa cggctgcgcc
     1621 gcggccatca tgcggatgtg acccatgtag tggattggcg agtcgcccat agcgcagtcg
     1681 aacacctcgt agtgctcctt gcgcagggcc ggtgcgccct tcaccgagtt gtgctcagcc
     1741 acgtactcgg taatcgcctg cacgtgctcg tcggcgtacc ccagcgtccg cagcgcccgc
     1801 ggcaccgtct ggttgacgat cgacatcgac ccgccaccga ccagcttttt ggtcttgacc
     1861 agcgctaaat caggctcaat tcccgtcgta tcacaatcca tgagaaggcc tatagtgcca
     1921 gtc
//
LOCUS       DATBRK010000298         3609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2073270, whole genome shotgun sequence.
ACCESSION   DATBRK010000298 DATBRK010000000
VERSION     DATBRK010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073270"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VIH70_08985"
     CDS             <1..545
                     /locus_tag="VIH70_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116534.1"
                     /translation="TLEDGRLSKWGLLRGVSAIPTVLAVNLLSFSLILASNVIFPVLG
                     PGIGLLAAIGALVGGLFFLGFAPTAAVRQQRGVLESIRRSGRAAMLPGSRHVLFCAIY
                     FFIALPVVVGLAPGGSEITANPTLSGWIFISAASVLHLAFMAALAFRWIGVEHAVPEE
                     PVRRRQAQRRQPASRQRGRR"
     gene            737..1615
                     /gene="rpsB"
                     /locus_tag="VIH70_08990"
     CDS             737..1615
                     /gene="rpsB"
                     /locus_tag="VIH70_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY8116535.1"
                     /translation="MPVVTRRELLEAGVHFGHQTRRWNPKMGRYLYGERSGIYIIDLE
                     KTLSGLEEAYAYARDLGRRGGVILFVGTKKQAQEVISEHAVRVGMPSVTTRWLGGMLT
                     NFQTVSRRLLRLRELREMERTGAFDFLPKREAIRLRHEKEKLERNLGGMQSLERLPDA
                     IFVIDTKKEHIAVNEARKLGISVIAIVDTNCDPDEVDFVIPGNDDAIRSVSLVTRLIA
                     DALAEGRQAGREAVVTRATGPEIEPEPAAAPKPTAAPAPAPAPAAEAAAAPEAAAGPE
                     PIVPDPASEADPTEAG"
     gene            1621..2229
                     /gene="tsf"
                     /locus_tag="VIH70_08995"
     CDS             1621..2229
                     /gene="tsf"
                     /locus_tag="VIH70_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY8116536.1"
                     /translation="MAEINARLVKELRDRTGAGMMQCKKALQEADGDLDKAEEILRTK
                     GLASAAGRAGRAANQGLIEAYIHFNNTVGAMVEVNCETDFVANTDEFKRLARDLALHI
                     ASPSAPRYVTRDEVAPEDLASERRIFEAQARELGKPDDVIPKIVEGKLKTFYAETVLL
                     DQPYVKDDSKTIQQLLDEMSSKVGEKVAVRRFVRYRLGEETG"
     gene            2240..2989
                     /gene="pyrH"
                     /locus_tag="VIH70_09000"
     CDS             2240..2989
                     /gene="pyrH"
                     /locus_tag="VIH70_09000"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY8116537.1"
                     /translation="MPERDDAQEAGRPLYHRVLLKLSGDAFAPMDTGYGISTQSTGLL
                     ARELADVVDLGVQCAVVVGGGNIFRGRQAPGIDRSRADYMGMLATVMNALALQDALEK
                     IHVPTRVQTAIHMAQIAEPYIPLRAIRHLEKGRVVVFAAGIGAPYFSTDTTAAQRALE
                     IGAQAILKGTKVDGVYTADPHVDPTAERFETIEYLDVLREGYQVMDATAISLCMENGL
                     PIIVFDFRSEGNIRRAVSGEPIGTLVHGGVR"
     gene            2986..3546
                     /gene="frr"
                     /locus_tag="VIH70_09005"
     CDS             2986..3546
                     /gene="frr"
                     /locus_tag="VIH70_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY8116538.1"
                     /translation="MTVDSALRAATEKMDKAIGVLKDELAGVRTGRATPALLQRVVVD
                     YYGTPVPIQQLASFSVPEPRTLVISPFDRNAIASMEKAIMSSDLGITPGNDGTVIRLS
                     FPPLTEERRKELIKLVHHRGEEGRVAVRNIRRHSKEELEKLEREGGISEDDLVRSEKE
                     LQKLTDKHISDIDEVVAHKDAELKEI"
BASE COUNT          610 a         1199 c         1287 g          513 t
ORIGIN      
        1 agacgctgga ggacggccgc ctctcgaagt ggggcctgct ccggggcgtc tcagccatcc
       61 cgaccgtgct cgccgtgaac ctgctctcgt tctcgctgat cctggcgagc aacgtgatct
      121 tccccgtgct cggccccggg atcgggctgc tggccgcgat cggcgcgttg gtcggcggcc
      181 ttttcttcct cgggttcgcg ccgacggcgg cggtccggca gcagcggggc gtcttggagt
      241 cgatccgacg ctccggccgg gcggcgatgc tgcccgggag ccgccacgtc ctgttctgcg
      301 cgatctactt cttcatcgcg ctgccggtgg tggtcggcct ggcgccaggc gggtccgaga
      361 tcaccgcgaa cccgacgctc tccggctgga tcttcatctc ggccgcgagc gtcctgcacc
      421 tggcgttcat ggcagcgctc gcgttccgat ggatcggcgt ggagcatgcc gtgcccgagg
      481 agcccgtgcg gcgccggcag gcccagcgcc gccagccggc ctcccgtcag cgcgggcgcc
      541 gctagccgtc cgggcgagcc ggaacatctg cgctggtaga ctgcgcgttc gccggcgttt
      601 cgacgccgag cacacacgcc cggggatctc gttcggggtc ccggtccagc gataggacgc
      661 gagacgctcg cgcccgcgag gaaccgggcc gacggtggcc gaaccgggcg gagggacaac
      721 cggaaggagg cgcaccatgc ccgtcgtcac caggcgggag ctcctcgagg caggggtgca
      781 cttcgggcac cagaccagac gctggaaccc gaagatgggc cggtacctgt acggggaacg
      841 ctccgggatc tacatcatcg acctcgagaa gaccctgtcg ggtctcgagg aggcctacgc
      901 ctacgcgagg gatctcggcc gccgcggcgg ggtgatcctg ttcgtcggga cgaagaagca
      961 ggcgcaggag gtcatctccg agcacgccgt acgggtcggg atgccgtccg tcacgacccg
     1021 ctggctcggc ggcatgctca cgaacttcca gacggtctcg cgacgcctgc tccgtctgcg
     1081 cgagcttcgc gagatggaac ggacgggcgc gttcgacttc ctccccaagc gggaggcgat
     1141 ccgcctgcgg cacgagaagg agaagctcga gcgcaacctc ggcggcatgc agagcctcga
     1201 aaggctcccc gacgcgatct tcgtgatcga caccaagaag gagcacatcg cggtcaacga
     1261 ggcgcgcaag ctcggcatct cggtcatcgc gatcgtggac acgaactgcg atccggacga
     1321 ggtggacttc gtgatcccgg gcaacgacga cgcgatccga tcggtctcgc tcgtcacacg
     1381 tctgatcgcg gatgccctcg ccgagggacg gcaggcgggc cgggaggccg tcgtgacgcg
     1441 cgcgaccggt cccgagatcg agcccgagcc cgccgcggcg ccgaagccga cggccgcccc
     1501 ggcccccgcc cccgccccgg ccgccgaggc tgcggcggcc cccgaggccg ccgccgggcc
     1561 cgagccgatc gtccccgacc cagcctccga ggccgatccg acggaagcgg gctgaagacg
     1621 atggcggaga tcaacgcgcg gctggtgaag gaactccgtg atcgcaccgg cgccggcatg
     1681 atgcagtgca agaaggcgct tcaagaggcg gacggcgatc tcgacaaggc ggaggagatc
     1741 ttgcggacga aggggctcgc gtcggcggcc ggccgcgcgg gtcgtgcggc gaaccagggg
     1801 ctcatcgagg cctacatcca cttcaacaac acggtcggtg cgatggtgga ggtcaactgc
     1861 gagacggact tcgtcgccaa cacggacgag ttcaagcggc tcgccaggga cctggcgctc
     1921 cacatcgcga gcccctcggc gccgaggtac gtcacccgtg acgaggtggc cccggaggat
     1981 ctcgcgtccg agcgacgcat cttcgaggcg caggcccggg agttgggcaa gcccgacgac
     2041 gtgatcccca agatcgtgga gggcaagctg aagacgttct acgcggagac ggtcctgctc
     2101 gaccagccgt acgtgaagga cgactcgaag acgatccagc agctcctcga cgagatgtcg
     2161 tccaaggtcg gggagaaggt ggccgtgcgt cggttcgtcc ggtatcggct cggcgaggag
     2221 accgggtagg gtggacctca tgccggagcg ggacgacgcg caggaggccg gccggccgct
     2281 ctaccaccgc gtactgctca aactctccgg ggacgccttc gccccgatgg acaccggcta
     2341 cgggatctcg acgcagtcca ccggactgct cgcgcgcgag ctcgccgacg tggtcgacct
     2401 cggggtgcag tgcgccgtcg tcgtcggcgg cggcaacatc ttccgggggc gccaggcgcc
     2461 ggggatcgat cgctcccgcg cggactacat gggcatgctc gccaccgtga tgaacgccct
     2521 cgcgctgcag gacgcgctgg agaagatcca cgtaccgacg cgcgtccaga cggcgatcca
     2581 catggcgcag atcgctgagc cgtacatccc tctccgcgcg atcaggcatc tggagaaggg
     2641 tcgggtcgtc gtgttcgccg ccgggatcgg cgcgccgtac ttctcgacgg atacgaccgc
     2701 cgcccaacgt gcgctcgaga tcggcgcgca ggcgatcctg aaggggacga aggtcgacgg
     2761 cgtgtacacc gcggacccgc acgtggaccc cacggcggag cggttcgaga cgatcgagta
     2821 cctcgacgtg ctccgcgagg ggtatcaggt catggacgcc accgcgatca gcctctgcat
     2881 ggagaacggc ctgccgatca tcgtgttcga cttccgatcg gaggggaaca tccgccgagc
     2941 ggtctccggc gagccgatcg ggacgctggt gcacggtggt gttcgatgac ggtcgactcg
     3001 gcgctgaggg cggccacgga gaagatggac aaggcgatcg gggtgctgaa ggacgagctc
     3061 gccggggtcc ggaccggacg cgcgaccccc gcgttgctgc agcgggtggt cgtggactac
     3121 tacggcacgc cggtgccgat ccagcagctg gcgtcgttct cggtgcccga accgcggacc
     3181 ctggtgatct cgccgttcga ccggaacgcg atcgcctcga tggagaaggc catcatgtcg
     3241 agcgacctgg ggatcacccc gggcaacgac ggcaccgtga tccggctgag cttcccgccc
     3301 ctgaccgagg agcgccgcaa ggagctgatc aagctggtcc atcaccgcgg cgaggagggc
     3361 agggttgccg ttcggaacat ccggcggcac tcgaaggaag agctcgagaa acttgagcgc
     3421 gaaggtggca tctcggaaga tgacctcgtg cgctccgaga aggagcttca gaagctcacc
     3481 gacaagcaca tctcggacat cgacgaggtc gtggcccaca aggatgccga gctcaaggag
     3541 atctgagcgt gccggaggac gaggaacgga acgagggcga agacctcttc gaggatctcg
     3601 acaagttct
//
LOCUS       DATBRK010000299         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_631399, whole genome shotgun sequence.
ACCESSION   DATBRK010000299 DATBRK010000000
VERSION     DATBRK010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631399"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VIH70_09010"
     CDS             <1..1026
                     /locus_tag="VIH70_09010"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8116539.1"
                     /translation="RDVKPSNVLLPPDGGVKLADMGIARLLSPEALTATLSVRGTAAY
                     ISPEQVRGDLVDSRADLYSLGCVLFEMLTGRTPFEGDLAALSYAHTHSQAPRVRSINP
                     AVPAAIDELLATMLEKDPARRPPTGEEVQRSLASAVSHDVEPTAVIEPVPLPPSPLPR
                     EPAGGRRRPGPKAWRAVVIAVCGLATLLFIALLAGREPDGQADASAPSGHLSSSPATQ
                     QPSPVETGSPTPDQPSPVQTISPVPEQPSPHEAAAVVLDTVGEGIEMGEITDHFDHDI
                     RHTIDEILRDTDKGEDVDGLLEKVDDLREKVSEALEKGEITSADRASAIDDALLRFEE
                     ALRDAEE"
     gene            complement(1082..1339)
                     /locus_tag="VIH70_09015"
     CDS             complement(1082..1339)
                     /locus_tag="VIH70_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116540.1"
                     /translation="MTAYVLVQTATQDDRIASLLRELPGIVFAEDVRGPYDALALVRS
                     DAGGDTFQAIVDQIRGLPGVIHALAAPLVQRPVELVTNTAA"
     gene            1504..2103
                     /locus_tag="VIH70_09020"
     CDS             1504..2103
                     /locus_tag="VIH70_09020"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY8116541.1"
                     /translation="MDTSIGLRERKKLRTRTELSDAAFRLFAERGFDETTIEDIVEQV
                     EVSPRTFFRYFDSKEDVVIGFFDDLGLELRAMLAARPPEEPPFAALRTALGTLIDLYE
                     ERQDRVVAAKRLAHETPSIRARLLDKHARWENGVTEELVTRVGGDPSHEPRMRLVAAV
                     ALAAFSTAVSAWCTGGGRADLHALVDDALGAVGDGFDGA"
     gene            complement(2157..2576)
                     /locus_tag="VIH70_09025"
     CDS             complement(2157..2576)
                     /locus_tag="VIH70_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116542.1"
                     /translation="MAVRRVVVDHVLFVVADLEASRRLYTAALAPLGYEELYVQEDCV
                     SYGADGFDDFTICRGEPVTTAAHVAFDAADRAPVDAFFDAAIAHGATVRGEPGVWTQY
                     SERYYGAFVNDLYDNNVEAVWHAPEPVTDAPLRPGVP"
     gene            2674..2991
                     /locus_tag="VIH70_09030"
     CDS             2674..2991
                     /locus_tag="VIH70_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116543.1"
                     /translation="MADFVLLYSGGSMPETDEEKARVLKAWDGWYSELGGAIKDGGNP
                     FSPAAKTIGSDGKVSDVSGTPFTGYTILQADSLDAATGLAKNSPVLQGGGSVTVYEAF
                     DVM"
     gene            complement(3080..>3500)
                     /locus_tag="VIH70_09035"
     CDS             complement(3080..>3500)
                     /locus_tag="VIH70_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8116544.1"
                     /translation="ILGADALGGMPPGLRAHLERMDLADGTVLIRQGETPGDVYVLET
                     GRLRVETVTGRGTRMRLRTLRPGVVVGEVAMYSGDPRTADVVAEGPTVVMRLGRDSID
                     RIEADDPELAAALHRWLATTLAGRLGDSLRAFDALLD"
BASE COUNT          549 a         1231 c         1204 g          516 t
ORIGIN      
        1 cgggacgtca aaccgtcgaa cgtgctgctc ccgccagatg gcggcgtgaa gcttgctgac
       61 atggggatcg ctcgactcct gtcgcccgag gcgctcaccg cgaccctgag cgtgcggggc
      121 actgcggcgt acatctcccc agaacaggtg cgaggcgacc tcgtcgactc tcgagcggac
      181 ctgtactccc tgggctgtgt gctgttcgag atgcttaccg gccggacgcc gttcgaggga
      241 gaccttgcgg cactctcgta cgcgcatacc cacagccagg cgccccgtgt tcgctcgatc
      301 aaccccgcgg ttccggccgc gatcgacgaa ctcctcgcca cgatgctcga gaaggatccc
      361 gcccggcgcc cgccaaccgg agaggaggtc cagaggtctc ttgcctccgc cgtgagccac
      421 gacgtcgagc cgaccgcggt gatcgagccg gtgccactgc caccctcacc cttgcctcgc
      481 gagcccgcgg gcgggcgtcg gagacccgga ccgaaggcat ggcgggcggt cgtcatcgcc
      541 gtatgcggcc tcgccacgct cttgtttatc gccctgctgg caggccgcga gccggacggc
      601 caagccgatg cgtctgcgcc gtcggggcac ctcagcagtt cgcccgcgac gcagcagcca
      661 tcgcctgtgg agacaggctc gcccacaccg gatcagccgt cgcccgtgca gaccatttcg
      721 ccggtaccgg aacagccgtc gccgcacgag gccgccgcgg tcgttctcga taccgtcggt
      781 gagggcatcg agatggggga gatcaccgac catttcgacc acgacatccg tcacacgatc
      841 gacgagatcc ttcgcgacac cgacaagggc gaggacgtgg acgggttgct cgagaaggtc
      901 gacgatctgc gagagaaggt gtccgaagcc ctcgagaagg gcgagatcac gtccgcagac
      961 cgagcatcgg cgatcgacga cgcgctcctg aggttcgagg aggcccttcg cgacgccgaa
     1021 gagtgaacgc ggcactcccc atggagacgc tcgagatcga tctggtcccg aacctcgtgg
     1081 atcaggctgc ggtgttggtc accaactcca cgggccgttg gacgagcggc gccgcgagcg
     1141 cgtggatcac gcccggcaac ccgcggatct ggtccacgat cgcctggaac gtgtccccgc
     1201 cggcatctga gcgcacgagc gcgagcgcgt cgtacggccc gcggacgtct tcggcgaaca
     1261 cgatcccggg gagctctcgg agcaggctgg cgatccggtc gtcctgcgtg gcagtctgga
     1321 cgagcacgta tgcggtcatg ggttcgggct cctctccgtg ggtagccggt cccgggatgc
     1381 tgccgtgtcg cctctgcatg ggaccggtac ctccgtctcc gacaggcccc caccatagaa
     1441 cgtgggatgc catttggcaa cccctgccag attgcactcg acgccaagtg ctatgctgtg
     1501 cccgtggata cctcgatcgg cctcagggaa cggaagaagc tgcggacacg gacggagctc
     1561 tcggacgccg cgttccggct gttcgccgag cgggggttcg acgagacgac gatcgaggac
     1621 atcgtcgagc aggtcgaggt ctccccccgg acgttcttcc ggtacttcga ttccaaggaa
     1681 gacgtggtga tcgggttctt cgacgacctc gggctcgagc tgcgggcgat gctcgcggcc
     1741 cgtccccccg aggagccgcc gttcgcggcg ctccggacag ccctcgggac gctgatcgac
     1801 ctgtacgagg agcgccagga tcgcgtcgtg gccgcgaagc gcctggccca cgagacaccc
     1861 tcgatccggg cgcgcctgct cgacaagcac gcgcggtggg agaacggggt gaccgaggag
     1921 ctcgtgaccc gcgtcggggg cgacccctcg cacgagccgc ggatgcgact ggtcgcggcc
     1981 gtcgccctcg cggctttctc gaccgccgtg tctgcttggt gcaccggtgg cggtcgggcg
     2041 gacctccacg ccctcgtcga cgacgcgctc ggcgccgtcg gggacgggtt cgacggcgcc
     2101 tgagcgtcgg gtcgtccggg ggccgatcgc cggtccgcgg ggcgaacgac tgaaccctac
     2161 ggcacccccg gcctgagggg agcgtccgtg accggttccg gcgcatgcca gacggcctcg
     2221 acgttgttgt cgtacaggtc gttcacgaac gccccgtagt agcgctccga gtactgggtc
     2281 cacacgcccg gctcgcccct gacggtggcg ccgtgcgcga tcgccgcatc gaagaacgcg
     2341 tccaccggtg ctcggtcggc ggcgtcgaac gcgacgtgcg ctgcggtggt cacgggctcc
     2401 ccccggcaga tcgtgaaatc gtcgaacccg tccgccccgt aggagacgca gtcctcctgg
     2461 acgtacagct cctcataccc gagcggtgct aaggcggccg tgtacagacg ccggctggcc
     2521 tccagatcgg ccaccacgaa cagcacgtgg tccaccacga cccgccggac cgccatggcg
     2581 acacgatacg cgaaccctgc tgtcgaaagc ggcggccctc gttcgtcgta gggatgtcgg
     2641 acggacaggc cgggaggccg gaaggaggta tcgatggcgg acttcgtgct gctctacagc
     2701 ggcgggagca tgcccgagac ggacgaggag aaggcccgcg tcctcaaggc atgggacggc
     2761 tggtactcgg agctcggcgg ggccatcaag gacgggggca acccgttctc gccggcggcc
     2821 aagaccatcg ggagcgacgg caaggtgagc gacgtgtccg gcacgccctt caccggctac
     2881 accatcctcc aggcggactc gctggacgcg gcgaccgggc tggcgaagaa ctcgcccgtc
     2941 ctgcagggtg gaggcagcgt gaccgtgtac gaggccttcg acgtcatgta gcgggatcga
     3001 ggcggtcgcg ggcgctcgat cccgcgaccg cctccccgcg atcccgcacc gggttacgcc
     3061 gccgcgcgac cgcgcgtggc taatcgagca gcgcgtcgaa cgcccggagc gagtcgccca
     3121 acctccccgc aagcgtcgtc gcgagccaac ggtggagcgc cgcggcaagc tccggatcgt
     3181 cggcctcgat ccgatcgatc gaatctctgc cgagccgcat gaccacggtc ggcccctcgg
     3241 ccaccacgtc ggcggtgcgc gggtcccccg agtacatcgc gacctcgccc acgaccacgc
     3301 ccggtcgcag cgttcgaagg cgcatccgcg ttcctcgtcc ggtcacggtc tccacccgca
     3361 ggcggccggt ctccagcacg tagacgtcgc cgggcgtctc gccctggcgg atcagcacgg
     3421 tgccgtcggc gaggtccatg cgttcgaggt gggcgcgcag gccgggcggc atgccgccga
     3481 gcgcgtccgc gccaaggatc
//
LOCUS       DATBRK010000300         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1062064, whole genome shotgun sequence.
ACCESSION   DATBRK010000300 DATBRK010000000
VERSION     DATBRK010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062064"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VIH70_09040"
     CDS             complement(<1..528)
                     /locus_tag="VIH70_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8116545.1"
                     /translation="MSHAYLFSSESVTEGHPDKLADRISDAVLDAILALDPECRVACE
                     TLVTTGFVMVAGEISTSEYIEIPAVVRRTITEIGYTDANFGIDGDTCGVITSIQEQSP
                     DIARGVDEALEHREGRSVDELDALGAGDQGMMFGYACRETDELMPLPIAMAHRFAKRL
                     ADVRKAGVIPYLRPDG"
     gene            complement(611..1828)
                     /gene="coaBC"
                     /locus_tag="VIH70_09045"
     CDS             complement(611..1828)
                     /gene="coaBC"
                     /locus_tag="VIH70_09045"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY8116546.1"
                     /translation="MTTERSALRSVLGGRRVLLGVTGGIAAYKACILVRLLRLRGASV
                     RVVMTRSAERFVGPATFAALSDHRVYTDLWEEPGVVLHVRLAHEADVAVVAPATANVI
                     AKLAQGIADDLLTSTLLEASCPLVLAPAMHTGMWEHPATQANVSALSSRGARLVGPAH
                     GSLAAGDEGTGRMTEPEGIMQAIEDALAAAGDLAGTRIVVTAGPTWEPIDPVRFVGNR
                     STGKMGFAVAAEAFGRGAAVTLVVGPGTVEAPAGPALIRVTTAEEMRRAVLDAAEGAD
                     AVVMAAAVADFRPEIAADAKIKKDQGPPQVRLVPTRDILAELGAAGDTVLVGFAAETE
                     DVEASGREKLRRKGVQLLVANEVGREGTGFGSDTDRAAILAADGEDVGMREWTKPELA
                     SAICDRLAKLLTV"
     gene            complement(1874..2119)
                     /gene="rpoZ"
                     /locus_tag="VIH70_09050"
     CDS             complement(1874..2119)
                     /gene="rpoZ"
                     /locus_tag="VIH70_09050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY8116547.1"
                     /translation="MIEPKIDDLLAQVDSKYTLVTLSAKRAREINSYYNQLGEGRAEF
                     VPPLVETGLRNKPLSIALQEIAEGKINSERPDGVGIK"
     gene            complement(2116..2739)
                     /gene="gmk"
                     /locus_tag="VIH70_09055"
     CDS             complement(2116..2739)
                     /gene="gmk"
                     /locus_tag="VIH70_09055"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY8116548.1"
                     /translation="MFVVAGPSGVGKGTVVRLVRERNPSIHLSVSATTRAPRSSEREG
                     VDYRFVSEEAFDRMIEAGELLEWEEIFGHRSGTPAEPVRRALEAGIDVLLELDVTGAR
                     HVREAMPHAVLILLEPPTMQELERRLRSRGTETDEGLARRLAKADRELEQRDAFDVVI
                     VNDDAGRAADEVAAIIDASPQVDGLPEATPGHPAEGQAEAEKGSSKP"
     gene            2642..>2842
                     /locus_tag="VIH70_09060"
     CDS             2642..>2842
                     /locus_tag="VIH70_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116549.1"
                     /translation="MAETERWIEGFRSRTRRTTVPLPTPEGPATTNSLPSPAASGGYF
                     FANSPRSDSRCFAPRPRTRRLAE"
BASE COUNT          392 a         1046 c          983 g          421 t
ORIGIN      
        1 gccgtccggc cgcaggtacg ggatcacccc cgccttgcgc acgtcggcca gccgcttggc
       61 gaaccggtgc gccatcgcga tcggcagcgg catcagctcg tcggtctcgc ggcaggcgta
      121 cccgaacatc atcccctggt cgccggcgcc gagcgcgtcg agctcgtcga ccgatcgacc
      181 ctcgcgatgc tcgagcgcct cgtcgacgcc ccgcgcgatg tccggggact gttcctggat
      241 cgaggtgatc accccgcagg tgtcgccgtc gatcccgaag ttggcgtcgg tgtacccgat
      301 ctcggtgatc gtccggcgta cgaccgcggg gatctcgatg tattccgatg tcgagatctc
      361 gccggcgacc atcacgaacc ccgtcgtcac caacgtctcg cacgccacgc ggcactcggg
      421 gtcaagcgcg aggatggcgt ccaggaccgc gtcggagatc ctgtcggcga gcttgtcggg
      481 gtggccctcg gtgaccgatt cggacgagaa caggtacgcg tggctcatcg cggcggagca
      541 tagcagcggc ctccgacggc cctccgcacg gaggcgcggg atcggtcccg gggtcacgac
      601 gtgcccggcg ctagacggtg agcagcttgg cgaggcggtc gcagatcgcg gacgccagct
      661 cgggcttggt ccactcgcgc atgccgacgt cctcgccgtc ggcggccagg atcgcggcgc
      721 gatccgtatc cgagccgaac cccgtgccct cgcgcccgac ctcgttcgcc accaacagct
      781 gcacgccctt gcgccgcaac ttctcgcgcc cggaggcctc gacgtcttcg gtctcggcgg
      841 cgaagcccac gaggacggtg tccccggccg cgccgagctc cgcgaggatg tcgcgggtgg
      901 gaacgagacg gacctgcggc ggaccctggt ccttcttgat cttggcgtcg gccgcgatct
      961 cgggccggaa atcggcgacg gcggccgcca tcacgaccgc gtcggccccc tcggccgcgt
     1021 cgagcacggc acggcgcatc tcctccgcgg tggtcacgcg tatcagggcg ggacccgcgg
     1081 gggcctcgac cgtgccggga ccgaccacca gcgtgaccgc tgccccgcgt ccgaacgcct
     1141 ccgcggcgac ggcgaacccc atcttgcccg tcgagcggtt cccgacgaac cggacggggt
     1201 cgatcggctc ccacgtcggt ccggccgtca cgacgatgcg tgtgccggcg agatcgcccg
     1261 ccgcggcgag ggcatcctcg atcgcctgca tgatgccctc gggctcggtc atgcgacccg
     1321 tcccctcgtc gccggcggcc agcgagccgt gcgccggtcc gacgaggcga gcccctcgcg
     1381 acgacagggc ggacacgttg gcctgcgtcg ccgggtgctc ccacatcccc gtgtgcatcg
     1441 ccggcgccag gacgagcggg cagctggcct cgagcaacgt cgatgtgagc agatcgtccg
     1501 cgatgccctg cgcgagcttg gcgatcacgt tcgcggtggc cggcgcgacg acggcgacgt
     1561 cggcctcgtg ggcgagccgg acatgcagga cgacgccggg ctcctcccac aggtcggtgt
     1621 agacgcggtg atccgagagc gccgcgaagg tcgccgggcc gacgaaccgc tccgccgaac
     1681 gggtcatcac gacccgcacc gaggcgcccc gtaggcggag gagccgcacc aggatgcacg
     1741 ccttgtaggc ggcgatccca ccggtgacgc cgaggagcac gcgacggccc ccaaggacgg
     1801 agcggagggc ggagcgctcg gtcgtcacgc gggcctcctt cggccccgat cccttcgggg
     1861 cccggtcgtt cggttacttg atgccgaccc cgtccggccg ctcggagttg atcttcccct
     1921 cggcgatctc ctggagcgcg atcgacaggg gcttgttccg caacccggtc tcgaccaggg
     1981 gcggcacgaa ctcggcgcgg ccctcgccga gctggttgta ataggagttg atctcgcgag
     2041 cgcgcttggc cgacagcgtc acgagcgtgt acttggagtc gacctgagcg agcaggtcgt
     2101 cgatcttggg ctcgatcatg gcttcgagga tcctttctct gcctcggcct gtccctcggc
     2161 cgggtgtcct ggggtggcct cgggcaggcc atctacctgc ggagacgcat cgatgatagc
     2221 agcgacctcg tcggcggccc gcccggcgtc gtcgttcacg atcacgacgt cgaacgcatc
     2281 tcgctgctcg agctcccgat cggccttcgc caggcgccga gccagaccct catccgtctc
     2341 ggtgcctctc gaccgcagcc gacgctcgag ctcctgcatc gtcggcggct cgagcaggat
     2401 cagcaccgca tggggcatcg cctcgcgcac gtgcctggct ccggtcacgt cgagctcgag
     2461 gaggacgtcg atcccggcct cgagcgcccg gcggaccggc tcggcgggcg tccccgagcg
     2521 gtgcccgaag atctcctccc actcgagcag ctcacccgcc tcgatcatcc gatcgaaggc
     2581 ctcctcggac acgaagcgat agtcgacgcc ctcgcgctcg ctcgatcggg gcgcgcgcgt
     2641 cgtggccgag accgagaggt ggatcgaggg gttccgctcg cggaccaggc ggacgacggt
     2701 ccccttgccg accccggagg gtccggcgac gacgaacagc ctcccgagcc cggcggccag
     2761 cggtggctac ttcttcgcga actcgcccag gagcgactcg cgctgcttcg cgccgagacc
     2821 ccggacccgt cggctggcgg ag
//
LOCUS       DATBRK010000301         4157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2506584, whole genome shotgun sequence.
ACCESSION   DATBRK010000301 DATBRK010000000
VERSION     DATBRK010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506584"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1561
                     /gene="guaA"
                     /locus_tag="VIH70_09065"
     CDS             17..1561
                     /gene="guaA"
                     /locus_tag="VIH70_09065"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY8116550.1"
                     /translation="MASVPRRPVLVVDLGAQYAQLIARRIRECHVYSEIVAHDLPASE
                     LAARMPAGIVLSGGPASVYEAGAPALDPGVFELGVPVFGICYGHQLMAQTLGGEVAAT
                     GQREYGRTLLHVTEPGVLLEDLAADEQVWMSHGDAVTRTPEGFRVTSRTDAIPIASME
                     DPERSLYAVQFHPEVVHTPKGAAVMKRFLYDGCGLLPDWTPANVIERAVAAIRAQVGR
                     GEVLCALSGGVDSAVAALLVHKAIGEQLTCVFVDTGLLREGEPEQVEETFGRHFRVPL
                     VHVKAEDRFLGRLAGVTDPEDKRRRIGEEFIRVFEEVARDHRRARFLVQGTLYPDAIE
                     SGSETAARIKSHHNVGGLPAEMDLELVEPLRDLFKDEVRTVGAELGLPEEIVQRQPFP
                     GPGLAVRIVGEVDRENLTIVRAADHVVREEIRRAGLDRATWQAFCVLLADVRSVGVMG
                     DGRSYEHPVVLRAVTSQDAMTADWARLPHEVLDGIASRIVREVPGVNRVVYDITSKPP
                     GTIEWE"
     gene            complement(1587..2213)
                     /locus_tag="VIH70_09070"
     CDS             complement(1587..2213)
                     /locus_tag="VIH70_09070"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEY8116551.1"
                     /translation="MDDRDKRRVGEQIEPGGRPKAPGRLETLQRFVNTYNKDFPPAWD
                     RLGTPAKARAWLLAKRLVAPRVRITYDDLARLRELREALRALVAANHGSAVPRDALET
                     LCREGARARLRVGFGPRGATELEPVGRGADAAIATVLDILHEAQVTGVWPRLNACRKC
                     GYAFFDRSKNRSAAWCAMSICGNRTKNRAYRQRRAGETRQVSTSVGPG"
     gene            2254..2967
                     /locus_tag="VIH70_09075"
     CDS             2254..2967
                     /locus_tag="VIH70_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116552.1"
                     /translation="MPVMGSRWQRFGSMGLAFAAGVLTTVVVVAVASSEPEPAPPAKA
                     ASSATSSATPTVSGASPSPLLKIGGAPGAEPERTVDPLLLPYPYLSPTPPPSTQTELD
                     GTYMRILTLRDVGGARVGLPFRCLRCPPYRIDAGLSTIMFWHGAWFLHHQISGFKTQG
                     SFVVEGDRLTLFNDPNCPQGTGVYRFEKTAHSLRLETIEDDCPWSGERADDLTRVRWT
                     DAHVCVRWIKNLWPGAIAC"
     gene            complement(2993..3667)
                     /locus_tag="VIH70_09080"
     CDS             complement(2993..3667)
                     /locus_tag="VIH70_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEY8116553.1"
                     /translation="MAETEILEGSAVPARPPARRRRRRSGRILRIAGLVLIAAALLLT
                     GYLWWNLWGTGFATQRAQEDLRPGFERNVASLIPADAPDRVVKVPGDAVAIIRIPKID
                     VDYVVVEGTDTEALKKGPGHYTDTAYPWQDTGRVGIAGHRTTYGAPFWSLNELRVGDR
                     IVLATEYGIFNYRVARTVVTPPSGILPSGDYVLRQTTGPSLVLTTCNPRFSASARLVV
                     IADRVD"
     gene            3777..>4157
                     /locus_tag="VIH70_09085"
     CDS             3777..>4157
                     /locus_tag="VIH70_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY8116554.1"
                     /translation="MAVRSPLVDALNPVQAEAVLHTEGPVLIVAGAGSGKTRALTHRI
                     AYLIREHGVSPYAILAITFTNKAAREMAARVEGLLGQRIAKGMWILTFHSACARILRR
                     EHDHLGLPRSFTIYDEGDTERLLSS"
BASE COUNT          594 a         1439 c         1501 g          623 t
ORIGIN      
        1 atgggtcact aggagcgtgg cctcggtccc ccggcgcccg gtcctggtcg tcgatctcgg
       61 ggcgcagtac gcgcagctga tcgcgcgcag gatccgcgaa tgccatgtct actccgagat
      121 cgtggcgcac gacctccccg cctccgagct cgccgcgcgc atgccggcgg gcatcgtgct
      181 ctcgggtggc ccggcgtcgg tgtacgaggc cggggcgccg gcgctggatc cgggagtgtt
      241 cgagctcggg gtcccggtgt tcgggatctg ctacgggcac cagctcatgg cgcagaccct
      301 cgggggcgag gtcgcggcga cggggcagcg cgagtacggc cgcacgctgc tgcacgtgac
      361 cgagccgggg gtcctgctcg aggatctggc ggccgacgag caggtgtgga tgagccacgg
      421 cgacgccgtg acgcggaccc ccgaagggtt ccgcgtgacc tcccggaccg acgcgatccc
      481 catcgcgtcg atggaggacc ccgagcgatc cctgtacgcg gtgcagttcc acccggaggt
      541 cgtgcacaca cccaaggggg cggccgtgat gaagcggttc ctctacgacg ggtgcggtct
      601 cctgccggac tggacaccgg cgaacgtgat cgaacgcgcg gtcgccgcga tccgcgcgca
      661 ggtcgggcgg ggcgaggtgc tctgcgcgct ctcgggcggc gtcgactccg ccgtggccgc
      721 gctgctcgtc cacaaggcga tcggcgagca gctgacgtgc gtgttcgtcg ataccgggtt
      781 gctccgcgag ggcgaacccg agcaggtcga ggagacgttc ggccgccact tccgggtgcc
      841 gttggtgcac gtgaaagcgg aagatcggtt cctcggccgg ctcgcgggcg tgaccgaccc
      901 cgaggacaag cggcgccgga tcggcgagga gttcatccgc gtcttcgagg aggtcgcgcg
      961 cgaccaccgc cgtgccaggt tcctcgtcca ggggacgctg tatcccgacg cgatcgagag
     1021 cggttcggag accgcggcgc ggatcaagag ccaccacaac gtcggcggcc tcccggcgga
     1081 gatggaccta gaactcgtcg aaccgcttcg cgacctgttc aaggacgagg ttcgaaccgt
     1141 gggcgccgag ctcggcctgc ccgaggagat cgtgcagcgc cagccgttcc cgggcccggg
     1201 gctcgcggtc cggatcgtgg gcgaggtcga ccgcgagaac ctgacgatcg ttcgggcggc
     1261 cgaccacgtg gtgcgggagg agatccggcg cgccgggctc gaccgggcga cgtggcaggc
     1321 gttctgcgtg ctgctcgcgg acgttcggag cgttggggtg atgggagacg ggaggtccta
     1381 cgagcatccg gtcgtgctcc gggccgtgac aagccaggat gcgatgacgg ccgactgggc
     1441 tcggctgccg cacgaagtgc tcgacgggat cgcgagccgg atcgtcaggg aggttcccgg
     1501 cgtgaaccgc gtcgtctacg acatcacgag caagccgccc ggcacgatcg agtgggagta
     1561 gcgccgagcc cggtggtccg ccgatgtcat cccgggccca ccgaggtcga cacctggcgc
     1621 gtctcccccg cccgccgctg tcgataggcg cgattcttcg tgcggttgcc gcagatcgac
     1681 atcgcgcacc acgcggcgga acggttcttc gagcggtcga agaaggcgta cccgcacttc
     1741 cggcaggcat tgagcctcgg ccacaccccg gtgacctgcg cctcgtgcag gatgtccagg
     1801 acggttgcga tcgcggcgtc cgccccgcgt ccgaccggct cgagctccgt cgcgccgcgc
     1861 ggtccgaacc ccacccggag ccgggcccgg gctccctcgc ggcagagcgt ctcgagggca
     1921 tcgcgcggca cggcgctccc gtggttcgcg gcgacgagcg cccggagcgc ctcgcggagc
     1981 tcgcgcagcc gggcgaggtc gtcgtacgtg atccgcacac gcggcgcgac gagacgcttc
     2041 gcgagcagcc acgcccgcgc cttcgccggc gtaccgagcc ggtcccacgc cggcgggaag
     2101 tccttgttgt acgtgttcac gaaccgttgc agcgtctcga gccgtccggg cgccttgggt
     2161 cgaccgcccg gctcgatctg ctcgccgacg cgacgcttgt cgcgatcgtc catgtcgtga
     2221 cttgaccttt cgagagagac cagcataacg tggatgccgg tcatgggttc gcgttggcaa
     2281 cggttcggct cgatggggct cgcgttcgcg gcgggggtgc tcaccaccgt cgtagtcgtc
     2341 gcggtggcta gctccgagcc cgagccggca ccgcccgcca aagccgcgtc cagcgcgacg
     2401 tcctcggcga ctcccaccgt ctcgggagcg tccccgtctc cactcctcaa gatcggcgga
     2461 gccccgggcg cggagcccga gcgaacggtc gaccccctgc tcctgccgta cccgtacctg
     2521 tcgccgacgc ccccgcccag cacgcagacc gagctggacg gcacctacat gcggatcctg
     2581 acccttcggg acgtgggtgg ggcacgcgtc ggtctgccgt tccgatgcct gcgatgcccc
     2641 ccgtaccgga tcgacgccgg gctgagcacg atcatgttct ggcacggcgc gtggttcctg
     2701 catcatcaga tctccgggtt caagacgcag gggagcttcg tagtcgaggg cgatcgcctc
     2761 acgctgttca acgacccgaa ctgcccgcag ggcacgggcg tgtaccggtt cgagaagacg
     2821 gctcacagcc tccggttgga gacgatcgag gacgactgcc cgtggagcgg cgagcgcgcg
     2881 gacgatctca cgcgcgtgcg gtggaccgac gcccacgtct gcgtccggtg gatcaagaac
     2941 ctgtggcctg gcgcgatcgc ctgctgaagg atgaccgggc gcgacccggc cgctagtcga
     3001 cgcggtcggc gatcaccacg agccgggccg acgccgagaa acgcgggttg caggtagtca
     3061 ggacgaggct cgggcccgtc gtctgacgca ggacgtaatc gcccgatggc aggatccccg
     3121 atggcggggt gacgaccgtg cgtgccaccc ggtagttgaa gatcccgtac tcggtcgcga
     3181 gcacgatccg gtcgcccacg cggagctcgt tcagcgacca gaacggcgcg ccgtacgtcg
     3241 tccggtgtcc cgcgatgccc acccgcccgg tgtcttgcca cgggtacgcg gtgtcggtgt
     3301 agtggcccgg acccttcttg agggcctcgg tgtcggtgcc ctccaccacg acgtagtcga
     3361 cgtcgatctt cgggatccgg atgatcgcca ccgcgtcccc cggcaccttg accacccggt
     3421 ccggcgcgtc ggcagggatg aggctcgcga cgttgcgttc gaagccgggg cggaggtcct
     3481 cctgggcccg ctgggtcgcg aacccggttc cccacaggtt ccaccacaga tacccggtca
     3541 ggaggagcgc ggccgcgatc aggaccaggc ccgcgatccg caggatccgg ccgctgcggc
     3601 gccgccggcg acgggcgggt ggtcgggcgg ggacggcgct gccctcgagg atctcggtct
     3661 cggccatccc ttgaagtgtc gcaggttcgg tggcattcct tgagcgcgcg agctaccggc
     3721 gggcgatcct cggccgttcg gcgagggccg tccgaggcac acctacaatc agggccgtgg
     3781 ccgtccgttc gcccctcgtc gatgccctga accccgtgca ggccgaggcg gtcctgcaca
     3841 ccgagggtcc ggtcctgatc gtggcggggg ccgggtcggg caagacccgg gccctgaccc
     3901 accggatcgc ctacttgatc agggagcacg gcgtgagccc ctacgcgatc ctcgcgatca
     3961 cgttcaccaa caaggcggcc cgcgagatgg ccgcgcgggt ggagggcctg ctcgggcagc
     4021 ggatcgcgaa ggggatgtgg atcctcacgt tccattcggc ctgcgcgcgg atcctgcggc
     4081 gcgaacacga ccacctcggc ctgccgcgaa gcttcacgat ctacgacgag ggagacacgg
     4141 agcgcctgct ctcgagc
//
LOCUS       DATBRK010000302         7819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1640864, whole genome shotgun sequence.
ACCESSION   DATBRK010000302 DATBRK010000000
VERSION     DATBRK010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640864"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VIH70_09090"
     CDS             complement(<1..294)
                     /locus_tag="VIH70_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEY8116555.1"
                     /translation="MRYFDDDALAFLGDLKRHNDRAWFNEHKERYERSLRQPFLDFIS
                     DVGPELRSISPNLVADPRPSGGSLFRIHRDVRFSKDKSPYKTHAGAHFQLGGKG"
     gene            563..1609
                     /locus_tag="VIH70_09095"
     CDS             563..1609
                     /locus_tag="VIH70_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEY8116556.1"
                     /translation="MLTSRRVGLPAPSVECPRCGSGLVVAALAGGPSEYVLQLRSGTA
                     PTGGSGPMGEWLCRSCGLRWPDDRSPAEVALEAADPEPDPTAVSGELIELVDLPEIPG
                     STGVTSARVASALRDAREARGLTLSDAASATRIWDRYLQALETGASLEEFPAPIYARS
                     FLRAYAEFLGLETEIMVNRFDEDHPAGEAPILQPLPDPRPRRRVAVNALVFVSILALL
                     AMAVARLDAGRDRGLMPSLPVASPDASVGRSTLTRPPPAPRVEGIRAVLRLQDRCWVV
                     AVADGTTLEQGRTLEPGDRLVFRADRILELQLGSAGAVELEVNGERVRTGDLGEVVRL
                     ELRWKDGEVHTAFV"
     gene            1625..2494
                     /locus_tag="VIH70_09100"
     CDS             1625..2494
                     /locus_tag="VIH70_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY8116557.1"
                     /translation="MSTTDEWTFGGQTLVAPLRRVLVRAPDPADLARWREFGWLAEPD
                     PAIAAEQHGSLCDVLRGAGAEVVFAEGDVAGDPDAVYVFDPVIVSDRGALLLRPGKEG
                     RRGEPPAIAEDLRSLGVPVAFEMRAPATAEGGDTMWLDQRTLLVGRSYRTNDAGIDAL
                     REALPGVEVLAFDLPRDQGPQACLHLLSLLSPLDDDLVVAYPPLMPIRMLELLAERDI
                     EMVEVPGEEFGSMGPNVLALAPRVALAVDGNPQTRRRMEAAGVDVTVYEGSEISHKGE
                     GGPTCLTRPLLRG"
     gene            2552..3109
                     /locus_tag="VIH70_09105"
     CDS             2552..3109
                     /locus_tag="VIH70_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8116558.1"
                     /translation="MSSTASKTRLTAEERREEILGAAMEEFAHGGLDGTPTEAIARRV
                     GISQPYLFRLFRTKRDLFLAVIERCFESTLDRFRTAAEGHAGKDALHAMGRAYAGLLK
                     DRTRLLLQLQAYVACDDPEVRTIVRDGFGRLATFVAEASGAPAADVRRFFETGMLMNV
                     VAAMELDRVRQPWARDLMVPMEDER"
     gene            3201..4829
                     /locus_tag="VIH70_09110"
     CDS             3201..4829
                     /locus_tag="VIH70_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8116559.1"
                     /translation="MTTTQIRPVEPASEEAGPNPRRWRILAVLCVSLFVIVMDNTIVN
                     VALPTLARELDAGTSSLQWIVDAYTLVFAGLLLAAGGLGDRFGRKGALLAGLALFGAF
                     SAAGAFASSTGQLISARAFMGVGAALIFPTTLAIVVNVFTDPRERAAAIGIWTAIAGV
                     GVALGPISGGWLLEHFSWGSVFLVNVPVTLAGIVGTLVLVPRSRDPRAPRLDLPGLGL
                     SIAGVTLLVWSLIEAPSHGWISGTTIGGIAGAVVLLSVFAWWELRTTSPLLDVHLFRN
                     MRFTAASLAITLGFFALFGFIFLVTQYLQLVKGYSALQAGVRTLPFAFAMVAAAVTSP
                     KAAQRVGTKLVVAAGLTLMAGGFAIASTNDASTPYWVIAGAMILMGFGLGSAAAPATE
                     SIMASLPREKAGVGSAVNDTTREIGGTLGVAVLGSLMASVYGGRILDALSGTPLPAGL
                     RAAAGDSLATALEIAGGVGGAAGRGIALAAQDAFVHAFQIGSVVTGAVALAGAVLALL
                     FLPSRSREEDPIAIELEREPLRPRLEPVVERVEG"
     gene            4835..5566
                     /locus_tag="VIH70_09115"
     CDS             4835..5566
                     /locus_tag="VIH70_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8116560.1"
                     /translation="MRLAERRILITGGSSGIGLAVARALAPENTVVIAGRDERKLEEA
                     RASTPRLRALRLDVTSEDDARRALGWVQEQLGGLDLLVNSAGVFRSGAFDAPGAEGAA
                     QADVEINLLGSVRMTRLALPMLRRSDEGAVVLLSSALALVSAPRMAVYAATKAAIHSL
                     ARSLRAELDGQVKVFDVLPPWVESELSHEVRGAKITPDHVAGEIVAGLRKDRFEIRIG
                     RIRQLAVIGRISPRLADSIAARATG"
     gene            5630..6871
                     /locus_tag="VIH70_09120"
     CDS             5630..6871
                     /locus_tag="VIH70_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8116561.1"
                     /translation="MSGASPSQAGQPDELSPTDLAARAGISPEELERFVASGVLVPRD
                     GTRPFRTADVLKVRVASACEAGGLPMAGMAEAIRAGLLSFAFVESWPFEPEGSRRPQT
                     HLELAEEAGLPFESLQRLMEAFGFARPVPGDVVVEAERPIALLAGRLAELEIIDQAML
                     VRLCRVYAEAFRRIALAETEVYHTGVEVPLLRSGLGESRTMELASSLSPELTGMLDDA
                     VMAAYRRQQELTWTEHQIEHVEQALEDAGVSLPATPPTAMCFLDLSGYTRLTEERGDE
                     AAAALAGRLSDIVLQRSRLRGGEAVKWVGDGVMFRFGDPSGAVASALDMMEEIPAAGL
                     PPAHVGVAAGPVIRQGGDYYGRTVNLASRISDRAAEGQVLVSEPVVEMASVPGVTFVG
                     IGSIELKGLKGPVDLFEARRD"
     gene            complement(6901..>7819)
                     /locus_tag="VIH70_09125"
     CDS             complement(6901..>7819)
                     /locus_tag="VIH70_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY8116562.1"
                     /translation="NPSIDRLFEVERLVAGGIHRPLGFVQVAGGKGLNAARAARRLGG
                     DVRVAAILRGHAGRWLEETLQADGIGGAFVWTHGENRSSLSVADRSAGDLTEFYEHGP
                     VAPESAWAELAEAASGLFQRGGWLTISGSMPRGLRDGEYCDLVAEARAAGMRVALDAE
                     GEPLRLALDAGPDIVKVNAAEASGLLGEPVAEREDAMAASAKLRTLAGGEGHAGIVTR
                     GADGVAVAAPDGARYEGVLYEQGRYPVGSGDAFLGGLVVGLERGDPWPDALRLALGAA
                     AANAELPGAGVLDAARADELRERVEIREL"
BASE COUNT         1024 a         2720 c         2866 g         1209 t
ORIGIN      
        1 gcccttgccg ccgagctgga agtgcgcgcc cgcgtgcgtc ttgtacgggc tcttgtcctt
       61 ggagaagcgg acgtcgcggt ggatccggaa cagcgaacct cccgacggcc gcggatcggc
      121 gacgaggttg gggctgatcg atcgcagctc ggggccgacg tccgagatga agtcgaggaa
      181 cggctgccgg agcgaccgct cgtaccgttc cttgtgctcg ttgaaccagg cccggtcgtt
      241 gtgacgtttc agatcgccca ggaacgcgag cgcgtcgtcg tcgaagtacc tcatccgagc
      301 ctccttcggc ctccgtggtc cgcggatccg cgggcaacct accgagcgag ggtgccgggc
      361 gtcccgtggg atggggggaa cggcccgggg agtgcccgag actccgttga cttcgggcgc
      421 ggtacctgcc taaggtcgaa gtgaacgtcc ccatggccgg ggtttcaatg cgagggaagc
      481 tgtccgctgt ggcttgcgca ccccgtacgt caagcacgac ccttgagctg cgtggtgtcc
      541 gagactccgc ggtggaccag gcgtgctgac gtcccgccgc gttgggctac cggcgccgag
      601 cgtcgaatgc ccacgctgcg gatcggggct ggtcgtcgcc gccctggccg gcggtccgtc
      661 cgagtacgtg ctccagctcc ggagcggcac cgccccgacg ggcgggagcg gcccgatggg
      721 cgagtggctg tgccggtcgt gcggtctgcg ttggccggac gatcgatcgc cggccgaggt
      781 tgccctcgag gccgccgatc ccgaacccga cccgacggcg gtgtccggcg agctgatcga
      841 gctcgtcgat ctcccggaga tcccgggttc gaccggggtg acgtccgctc gagtcgcttc
      901 cgcgcttcgg gatgccaggg aggcgcgagg actcaccctg tccgatgccg cgagcgcgac
      961 gcggatctgg gatcggtacc tgcaggcgct cgagaccggc gcctcgctcg aggagttccc
     1021 cgccccgatc tacgcccggt ccttcctgcg cgcgtacgcg gagttcctgg ggctcgagac
     1081 cgagatcatg gtcaaccggt tcgacgagga ccatccggcc ggggaggccc cgatcctcca
     1141 gccgctcccc gatccacgac cacggcgacg ggtcgccgtc aacgcactcg tgttcgtttc
     1201 gatcctggcc ttgctggcga tggcggtcgc ccgactcgac gccggacgcg accgaggact
     1261 gatgccatcg cttcccgtcg cgtcgccgga tgcgtcggtg ggccgatcca ccttgacgcg
     1321 gccgccgccc gcgccgcggg tcgaaggcat cagggcggtc ttgcggctgc aggaccgctg
     1381 ctgggtggtg gccgtcgccg atggaacgac cctggagcag gggaggacgc tggaacccgg
     1441 cgatcggctc gtgttccgcg ccgaccggat cctggagctg cagctgggga gcgccggggc
     1501 cgtcgagctg gaggtgaacg gcgagcgtgt tcgtacgggc gacctcggcg aggtcgtccg
     1561 cctcgagctc cgctggaagg atggcgaggt ccacaccgcc ttcgtctgac gggttcgggg
     1621 catcatgtcg accacggacg aatggacctt cgggggacag acgctcgtcg cgccgctccg
     1681 ccgtgtgctc gtccgagctc ccgatcccgc cgacctcgcg cgatggcgcg agttcggatg
     1741 gctcgccgag ccggaccccg cgatcgccgc cgagcaacac gggtcgctct gcgacgtcct
     1801 ccgtggtgca ggcgccgagg tggtgttcgc ggagggcgac gtcgccggcg atcccgacgc
     1861 cgtctacgtg ttcgaccctg tgatcgtgtc cgatcgcggc gcgctgctcc tgcgaccggg
     1921 caaggagggc cgtcgcggcg agccgccggc gatcgccgag gacctacgct cgctcggcgt
     1981 gccggtcgcg ttcgagatgc gagcacccgc gaccgccgag ggcggcgaca cgatgtggct
     2041 cgaccaacgc acgctgctgg tcggccgtag ctaccggacc aacgacgctg gcatcgacgc
     2101 gctccgcgag gcgctgcccg gtgtcgaggt gctcgcgttc gacctgccac gcgaccaggg
     2161 accccaggcg tgcctccatc tgctctcgct gctttcgccg ctcgacgacg acttggtggt
     2221 cgcctacccg ccgctgatgc cgatccgcat gctcgagctg ctcgcggagc gcgacatcga
     2281 gatggtcgag gtgcccgggg aggagttcgg ctcgatggga cccaacgtcc tcgcgctcgc
     2341 tccacgcgtc gcactggccg ttgacggcaa cccgcagacg cggcggcgga tggaggcggc
     2401 gggcgtggac gtcaccgtct acgagggctc ggagatctcg cacaagggcg agggcggccc
     2461 gacctgcctc acccgcccac tgctccgcgg ctgacggctc ggcgcggagc ggatcttgac
     2521 attgaagtga gtgagtgcta acttcctcgg catgtcgtcc accgcctcga agaccaggct
     2581 caccgccgag gagcgccggg aggagatcct gggcgccgcg atggaggagt tcgcgcacgg
     2641 cgggctcgac ggcacgccca ccgaggcgat cgccaggcgg gtcgggatct cacagcccta
     2701 cctgttccgc ctgttccgaa cgaagaggga cctgttcctc gcggtcatcg agcgctgctt
     2761 cgagagcacg ctcgacaggt tccgaacggc ggccgagggc cacgccggca aggatgccct
     2821 gcatgcgatg gggcgggcgt acgcggggct cctcaaggat cgaacccggc tcctgctgca
     2881 gctgcaggcg tacgtggcgt gcgacgaccc cgaggttcga acgatcgtcc gcgacggttt
     2941 cgggcgtctg gcgacgttcg tggccgaggc ctcgggcgca cccgcggcgg acgtccgtcg
     3001 cttcttcgag accgggatgc tgatgaacgt cgtcgccgcg atggagctcg accgcgttcg
     3061 gcagccgtgg gcgcgagacc tgatggttcc gatggaggac gagcgttgag ccaacggccg
     3121 caccccacca cgtctctctc ttttttcgtt tccgaaggta gtgaccgttc actagtcgcc
     3181 gagcggaagg aggatccgcg atgaccacga cccagatccg gccggtggaa ccggcatccg
     3241 aggaggccgg cccgaacccg aggcgctggc ggatcctcgc cgtgctctgc gtctcgctgt
     3301 tcgtgatcgt gatggacaac acgatcgtga acgtcgccct gcccacgctc gcccgcgaat
     3361 tggacgcggg cacgagcagc ctgcagtgga tcgtcgatgc ctacaccctc gtgttcgccg
     3421 gcctgctgct cgccgcgggc ggcctgggcg accggttcgg ccgcaagggt gcgctgctcg
     3481 ccggcctggc gctgttcggc gcgttctccg cggccggcgc gttcgcctcg agcaccggac
     3541 agctgatctc agcccgcgcc ttcatgggcg taggcgccgc gttgatcttc ccgaccacgc
     3601 tggcgatcgt cgtcaacgtc ttcaccgatc cgcgcgagcg cgccgccgcg atcgggatct
     3661 ggaccgcgat cgcgggcgtg ggcgtggcac tcgggccgat ctccggcgga tggttgctcg
     3721 agcacttctc gtggggctcg gtcttcctcg tgaacgtgcc ggtcacgctc gccgggatcg
     3781 tcgggaccct cgtgctggtc ccgcgttcgc gcgatccgcg cgcgccacgg ctcgaccttc
     3841 ccggcctcgg gctctcgatc gccggggtga cgctgctggt gtggtcgctg atcgaggcgc
     3901 cgagccatgg ctggatctcg gggacgacga tcggcgggat cgccggggcg gtcgtgctcc
     3961 tgtcggtgtt cgcgtggtgg gagctgcgta ccacgtcgcc gctgctcgac gtgcacctgt
     4021 tccgaaacat gcggttcacc gcggcaagcc tcgcgatcac gctcggcttc ttcgcgctgt
     4081 tcgggttcat cttcctggtg acgcagtacc tgcagctggt gaagggctac tcggccctgc
     4141 aggcgggcgt gcggacgctg ccgttcgcgt tcgcgatggt cgccgcggcc gtgacatcgc
     4201 cgaaggcggc gcagcgtgtc gggaccaagc tggtggtggc ggccgggctc accctgatgg
     4261 cgggcgggtt cgcgatcgcc tcgacgaacg acgcctcgac gccctactgg gtcatcgcgg
     4321 gcgcgatgat ccttatgggg ttcgggctcg ggtccgcggc cgccccggcc accgagtcga
     4381 tcatggcctc gctgccccgc gagaaggcgg gcgtgggttc ggcggtgaac gacacgacgc
     4441 gtgagatcgg cggcacgctg ggggtcgcgg tgctcggcag cctcatggcc tcggtttacg
     4501 gcggtcggat cctcgacgcg ctctcgggca ccccgctgcc cgccggcctg cgggccgcgg
     4561 cgggcgactc gctcgccacg gcgctcgaga tcgccggcgg cgtgggcggc gccgcgggga
     4621 gaggcatcgc gctcgccgcg caggacgcgt tcgtccacgc cttccagatc gggtcggtcg
     4681 tgacgggcgc ggtggcgctc gccggcgccg tgctcgcgct cctgttcctt ccgtcacgtt
     4741 cgcgggagga ggatccgatc gcgatcgagc tcgagcgcga gccgctgcgg ccccggctgg
     4801 agccggtcgt cgagcgggtc gagggctgag cgcgatgcga ctcgccgaac gcaggatcct
     4861 gatcaccggt gggagctcgg ggatcgggct cgcggtcgcc cgggcgctcg cgcccgagaa
     4921 caccgtcgtg atcgccggca gggacgagcg gaagctcgag gaggcacgag cgagcacccc
     4981 gcgcctgcgg gcccttcggc tggacgtcac gtccgaggac gacgctcgtc gcgctctcgg
     5041 gtgggtccag gagcagctcg gcgggctgga cctgctcgtc aacagcgccg gcgtgttccg
     5101 gagtggggcg tttgacgcgc cgggagccga gggggccgcg caggcggacg tcgagatcaa
     5161 cttgctcgga tcggtgcgga tgacgaggct ggcgctgccg atgctgcgtc gctcggacga
     5221 gggggccgtc gtgctgctct cctcggcgct cgcgctcgtc tccgccccgc ggatggccgt
     5281 ctatgcggcc acgaaggccg cgatccactc gctcgcccgt tcgctgcggg cggagctcga
     5341 cggccaggtc aaggtgttcg acgtcctgcc gccgtgggtc gagagcgagc tctcgcacga
     5401 ggtcagggga gcgaagatca cgcccgatca cgtggccgga gagatcgtcg cggggctgcg
     5461 caaggatcgc ttcgagatcc gcatcggccg catcaggcag ctcgcggtga tcggccggat
     5521 cagcccgagg ctcgcggatt cgatcgcggc gcgggcaacc ggttgaaacg ccgttgtcgg
     5581 tccgggcgcg agtggccaag gcaccgccac cgcgacatac gatcggtgcg tgagcggcgc
     5641 gtctccatcg caggcggggc agcccgacga gctgtcaccc acggacctcg cggccagggc
     5701 cggcatctcg cccgaggagc tcgagcgctt cgtcgcgtcc ggggttctgg tcccgcgtga
     5761 cgggacccgg ccgttccgca cggcagatgt cttgaaggtc cgggtcgcga gcgcgtgcga
     5821 ggcgggcggc ctgcccatgg cgggcatggc cgaggcgatc cgggcgggcc tcctgtcgtt
     5881 cgcgttcgtc gagagctggc ccttcgagcc ggagggttcg cgccggccgc agacgcatct
     5941 cgagctcgcc gaggaggccg gtctcccgtt cgagtccctg cagaggctga tggaggcctt
     6001 cgggttcgcc cgacccgtgc cgggggacgt cgtcgtcgag gccgagcgcc cgatcgcgct
     6061 gctcgcgggg cgactggccg agctcgagat catcgaccag gcgatgttgg tgcggctctg
     6121 ccgcgtctac gcggaggcgt tccgacggat cgcccttgcc gagacggagg tctaccacac
     6181 cggggtggag gtgccgctgc tccgctcggg tctgggtgag agcaggacca tggagctcgc
     6241 gtccagcctg agcccggagc tgaccgggat gctcgacgac gccgtcatgg ccgcgtaccg
     6301 acggcagcag gagctcacct ggaccgagca ccagatcgag cacgtcgagc aggcgctcga
     6361 ggacgcgggc gtctcgctgc cggcgacgcc gccgacggcg atgtgcttcc tcgacctgtc
     6421 gggctacacc cgcctcaccg aggagcgcgg tgacgaggcg gcggcggcgc tcgcaggccg
     6481 gctctccgac atcgtcctgc aacgatcgcg tctgcgggga ggagaagcgg tgaagtgggt
     6541 cggtgacggc gtgatgttcc gcttcgggga tccgagtggc gccgtggcat cggccctcga
     6601 catgatggag gagatccccg cggcgggcct gccgcccgct cacgtggggg tcgccgccgg
     6661 cccggtgatc cgtcagggag gcgactacta cgggcgaacg gtgaacctcg cctctcggat
     6721 ctcggatcgc gccgcggagg gacaggtctt ggtcagcgaa ccggtcgtcg aaatggcctc
     6781 ggtgccgggc gtgaccttcg tcgggatcgg gtcgatcgag ttgaagggcc tgaagggtcc
     6841 cgtcgacctg ttcgaagcgc gccgcgactg aggttccggc cggggtactc cgagacgcga
     6901 tcagagctcg cggatctcga ctcgctcccg gagctcgtcg gccctggccg cgtcgagcac
     6961 gcccgcgccc gggagctccg cgttcgccgc ggcggcgccg agcgcgagcc gcaacgcgtc
     7021 cggccaggga tcgccgcgct cgagcccgac gaccaggccg ccgaggaacg cgtcgccgct
     7081 gccgaccggg tagcggcctt gctcgtagag gacgccctcg taccgcgcgc cgtcgggcgc
     7141 ggccaccgcc accccgtccg caccgcgggt cacgatgccc gcatggccct cgccgccggc
     7201 gagcgttcgg agcttcgcgg acgcggccat cgcgtcctcc cgctcggcta cgggctcgcc
     7261 gagcagcccc gaggcctccg cggcgttcac cttcacgata tcgggaccgg cgtccagggc
     7321 gaggcgaagt ggctcaccct ccgcgtcgag cgcgacccgc atgccggccg ctcgagcctc
     7381 ggccaccagg tcgcagtact cgccgtcgcg cagcccacgg ggcatcgacc ctgagatcgt
     7441 gagccagccg ccccgctgga acaggccgct cgccgcctcc gcgagctcgg cccacgcgga
     7501 ctccggcgcg accggaccgt gctcgtagaa ctccgtcagg tcgcccgcgc tccggtcggc
     7561 gaccgacaaa gacgaccggt tctcgccatg ggtccagacg aacgcgccgc cgatcccgtc
     7621 ggcctgcagg gtctcctcga gccacctccc cgcgtgtcca cgcaggatcg ccgcaacgcg
     7681 cacgtccccg cccaaacgac gggccgcgcg cgccgcgttc aggcccttgc cgccggcgac
     7741 ctgcacgaac ccgagcggac ggtggatccc gccggccacg agccgttcga cctcgaacag
     7801 cctgtcgatc gaggggttc
//
LOCUS       DATBRK010000303         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_914343, whole genome shotgun sequence.
ACCESSION   DATBRK010000303 DATBRK010000000
VERSION     DATBRK010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914343"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1007
                     /locus_tag="VIH70_09130"
     CDS             87..1007
                     /locus_tag="VIH70_09130"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY8116563.1"
                     /translation="MPTKVLLAAPRGYCAGVERAVEAVERALETHGRPVYVRKQIVHN
                     AHVVRDLEAMGAVFVEEETEVPEGAVVVLSAHGVAPQVYANAERRQLTVLDATCPLVT
                     KVHLEARRFADHGRTIVLIGHAGHEEVVGTTGQAPGGTVLVQNVDEARSVEVLDPENL
                     SYLTQTTLSVDETNEIVEALRERFPSIQGPPREDICYATQNRQDAIKEVARRSDVVLV
                     IGSDNSSNSNRLAEVARDLGTPAYLVDDETEVDPAWLDGAVVVGLTSGASAPERLVDR
                     LLAWLGAQGATEVEELRITEEHLRFSDVRV"
     gene            complement(1047..1610)
                     /locus_tag="VIH70_09135"
     CDS             complement(1047..1610)
                     /locus_tag="VIH70_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY8116564.1"
                     /translation="MDAFVYLRVRPGRVEDVVAQLESVGGVRAAVAVVGAWDVLAAVQ
                     GPDLLGISEEVVRGIHPIEGVERTMTAPVVPRHVSTSPAEDGTRTPIPMQEPGPACFV
                     HVKASPGAAARVADTIAGVEEVSAVAMIAGEHDLIVEIPFPWEQAAPVIVNRLLPIDG
                     VQDTRTLVAIQFPGPDEEDRDQFSAWS"
     gene            complement(1661..2851)
                     /locus_tag="VIH70_09140"
     CDS             complement(1661..2851)
                     /locus_tag="VIH70_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEY8116565.1"
                     /translation="MDDQPKSVKDLLVEAKDASELMVDLAYAAVFFNDDKLAREVERL
                     EERMNGHLRRLREVAMLAARSPEDAEAMAGVLWIAGAVEKIGDAASDIGRVIEARLGI
                     PDALRRDLRHADEMTARVKVRDSAPVVGRALRELSLPTETGMWVMAIRSGIEWQFDPG
                     PDDVLSEADVLLVRGPEEGVTLLRELAGAPARPQPEEDDAPPLNELDRAVDILVELKD
                     LSEAAVGLAYSSILFNNRALAAEVGALEARSDTLHDELESWVLRAAPEARNPDELRGL
                     LRLGSASESVCDAARDLTWYVEHGEPLHPVVQIALEETEETSAETVVEAGTPADGRSL
                     KELRLETETGMFVLAVQRGARWIYRPRGPFVLRAGDRLIAVGPEEGAEALDALCRVSA
                     PAAS"
     gene            complement(2844..>3758)
                     /locus_tag="VIH70_09145"
     CDS             complement(2844..>3758)
                     /locus_tag="VIH70_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY8116566.1"
                     /translation="SSALWLAVLSVLASAAFGEPSISLWRLATVSIVGGAIGSALILM
                     ITLTLSVLSYRRGWDLDSVSTPMVTALGDMTTLPSLFLATYLLRSDLVAVPAAVISIA
                     LAVYAAIRSYTVADRVIRRIIVEMTAVILLTPILDIVAGTLLRARQEQLVAVPVLLAL
                     IPPFVSQAGALGGIFTSRTASKLQIGVITARGLPEIPALVDASIVSALSIAVFATVGV
                     VAYVLGVATDLVGMPSFPTLVAGTLLAGLIVTPITLVAGYYLAIGTFRFGLDPDDQSV
                     PIITSVMDLAGVAVVLFVMTSLGVLPNG"
BASE COUNT          593 a         1353 c         1310 g          502 t
ORIGIN      
        1 ctcacgacgg tcatcctgcc aggttggcac gagccaacga catgcccctt gaggctccag
       61 tctgttatgg cggctaccat ggggacgtgc cgaccaaggt cctgctcgcg gctccccgcg
      121 gctactgcgc gggggtcgag cgcgccgtcg aggcggtcga gcgagcgctc gagacgcacg
      181 gccgtcccgt ctacgtccgc aagcagatcg tccacaacgc gcacgtggtc cgcgatctcg
      241 aggccatggg cgcggtcttc gtcgaggagg agaccgaggt gcccgagggt gccgtggtcg
      301 tcctgtcggc gcacggggtc gcgccgcagg tgtatgccaa cgccgaacgc cgccagctca
      361 cggtcctgga cgcgacgtgc ccgctcgtca cgaaggtgca tctggaggcc cggcggttcg
      421 ccgaccacgg acggacgatc gtcctgatcg gccacgccgg tcacgaggag gtcgtgggca
      481 cgacgggcca ggcacccggc gggacggtcc tggtgcagaa cgtcgacgag gcgcgctcgg
      541 ttgaggtgct cgatcccgag aacctctcgt acctcacgca gaccacgctg tcggtcgacg
      601 agaccaacga gatcgtcgag gcccttcgcg aacggttccc ctcgatccag gggccgccgc
      661 gagaagacat ctgctacgcg acccagaacc gccaggacgc gatcaaagag gtggcgcgtc
      721 gctccgacgt cgtgctcgtc atcggctcgg acaactcgtc gaactcgaat cgcctcgccg
      781 aggtcgcgcg cgatctcggg accccggcct acctcgtcga cgacgagacc gaggtcgacc
      841 cggcgtggct cgatggcgcg gtggtggtcg gcctcacgag cggggcctcg gcgcccgagc
      901 gactcgtcga tcggctgctc gcctggctcg gcgcccaggg ggcgaccgag gttgaggagc
      961 tccgcatcac cgaggaacac ctgcggttct ccgacgtccg cgtctgaccc gaacctcgtc
     1021 ggtcgatcgg gcgaagctgg agcgtgtcag ctccaggcgg agaactgatc gcggtcctcc
     1081 tcgtcgggtc cggggaactg gatcgcgacc agggttcgcg tgtcctggac gccgtcgatg
     1141 gggaggagcc ggttcacgat aaccggcgcc gcctgctccc acgggaacgg gatctcgacg
     1201 atgaggtcgt gctcgcccgc gatcatcgcg acggccgaga cctcctcgac cccggcgatc
     1261 gtgtcggcga cccgagcggc cgcaccgggc gaggccttca cgtgcacgaa gcacgccggt
     1321 ccgggttcct gcatcgggat cggcgtccgc gtcccgtcct cggccggcga cgtggagacg
     1381 tggcggggga cgaccggcgc tgtcatggtc cgttcgacgc cctcgatcgg atggatcccc
     1441 cgaacgacct cctccgagat gccgagcaga tcgggaccct gcaccgccgc gaggacgtcc
     1501 caggcaccca cgaccgcgac cgccgcgcga acgccaccca cgctctcgag ctgggcgacc
     1561 acgtcctcga ccctccccgg acgaacccgc aggtagacga acgcgtccac gcaggcaggc
     1621 taggtccgcg acccgcacgc ggcaaggacc ggcgccgggc tcaggacgcg gcgggagccg
     1681 agacgcggca cagcgcgtcg agcgcctcgg cgccctcctc gggtccaacg gcgatcagcc
     1741 ggtcgccggc gcgcagcacg aacggtcctc gcggccggta gatccagcgg gcgccgcgct
     1801 gcaccgcgag cacgaacatc cccgtctcgg tctcgagccg gagctccttc aggctccggc
     1861 cgtcggccgg ggtcccggct tcgacgaccg tctccgcgga ggtctcctcg gtctcctcga
     1921 gcgcgatctg gacgacgggg tgcagcggct ctccgtgctc cacgtaccag gtgaggtcgc
     1981 gcgcggcgtc gcacaccgac tcgctcgcgc tcccgagccg gagcagcccg cgcagctcgt
     2041 ccgggttccg ggcctccggc gccgctcgga gcacccagga ctcgagctcg tcgtggagcg
     2101 tgtcgctgcg ggcctcgagc gccccgacct cggcggccag cgctcggttg ttgaacagga
     2161 tcgaggagta ggccaggccg acggccgcct cggacaggtc cttcagctcc accaggatgt
     2221 cgaccgcgcg gtcgagctcg ttcagcggcg gtgcgtcgtc ctcctcgggt tggggtcggg
     2281 ccggcgcccc ggcgagctcg cggagcaggg tcacgccctc ctcggggccc cgcaccagca
     2341 ggacgtcggc ctccgagagc acgtcgtcgg ggccggggtc gaactgccac tcgatcccgc
     2401 tgcggatcgc catcacccac atccccgtct cggtcggcag cgagagctcg cgcagcgcgc
     2461 gaccgaccac cggggccgag tctcggacct tcacccgtgc ggtcatctcg tcggcgtgcc
     2521 ggagatcgcg ccggagcgcg tcggggatcc cgaggcgggc ctcgatgacc cgcccgatgt
     2581 ccgaggcggc gtccccgatc ttctccacgg cgcccgcgat ccacaggacg ccggccatcg
     2641 cctccgcgtc ctccgggctg cgggcggcga gcatcgcgac ctcgcgcagg cggcggagat
     2701 ggccgttcat ccgctcctcg aggcgctcca cctcccgagc gagcttgtcg tcgttgaaga
     2761 agaccgcggc gtacgcaagg tccaccatca gctcggaggc atccttcgcc tcgacgagca
     2821 gatccttcac gctcttcggt tggtcatcca ttcgggagca cccccaatga tgtcatgacg
     2881 aacagaacga cggccacgcc cgcgaggtcc atcacgctcg tgatgatcgg cacgctctgg
     2941 tcgtcggggt ccaggccgaa ccggaaggtc ccgatcgcga ggtagtaccc cgcgaccagc
     3001 gtgatcgggg tcacgatcag cccggcgagg agggtcccgg cgaccagggt cgggaacgag
     3061 ggcatcccca cgagatcggt cgccaccccg agcacgtacg ccacgacgcc gaccgtcgcg
     3121 aagacggcga tcgacagcgc cgagacgatc gaggcgtcga cgagcgcggg gatctcgggc
     3181 agcccgcggg ccgtgatgac accgatctgg agcttggagg cggtgcgcga ggtgaagatg
     3241 ccgccgaggg cgccggcctg agacacgaag ggcgggatca acgccagcag caccggcacc
     3301 gccacgagct gttcctgtcg agcccgcagc agcgttcccg ccacgatgtc caggatcggc
     3361 gtcagcagga tcaccgcggt catctccacg atgatcctgc ggatcacccg gtcggcgacc
     3421 gtgtaggagc ggatcgccgc gtacacggcc agtgcgatcg agatcacggc ggccgggacg
     3481 gcgaccaggt ccgaccgcaa caggtacgtc gcgaggaaca ggctcgggag cgtcgtcatg
     3541 tcgccgagtg cggtgaccat cggcgtcgag accgagtcca gatcccagcc gcgacggtag
     3601 gacaggaccg acagcgtcag cgtgatcatc aggatcagcg ccgacccgat cgcgccgccg
     3661 acgatcgaga ccgtcgcgag gcgccacagc gagatcgacg gctcgccgaa cgccgccgag
     3721 gcgagcaccg acaggaccgc gagccacagg gccgacga
//
LOCUS       DATBRK010000304         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1062838, whole genome shotgun sequence.
ACCESSION   DATBRK010000304 DATBRK010000000
VERSION     DATBRK010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062838"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VIH70_09150"
     CDS             complement(<1..264)
                     /locus_tag="VIH70_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY8116567.1"
                     /translation="MRIAVVHPQTPFVRGGAETHTESVVGALREAGHEAEEVAVAGKW
                     YPATEIVHQMAVWRALDLSESNGLPIDMVIALKFPAYLVPHERK"
     gene            complement(261..620)
                     /locus_tag="VIH70_09155"
     CDS             complement(261..620)
                     /locus_tag="VIH70_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116568.1"
                     /translation="MTSENDARSALEREIYWAEDASVRSRMDTSEVRDLLHDFAMLMR
                     ADEKQAVPRGEPELGSGSRMRRKTKVLQFRLLRPMRRRNDRLLAEFGELAAALADRVV
                     ALETEVKELRSKLGDVE"
     gene            complement(691..996)
                     /locus_tag="VIH70_09160"
     CDS             complement(691..996)
                     /locus_tag="VIH70_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8116569.1"
                     /translation="MIARMWFGRTKAADYDGYLAYLEESGVAELHATPGNRGVMVLRR
                     LDGDEAEFGVMSLWDSLDDVKAFAGQDVDAAHYYPEDERFLLEFEPRVKHFEVTRER"
     gene            complement(1018..>1792)
                     /locus_tag="VIH70_09165"
     CDS             complement(1018..>1792)
                     /locus_tag="VIH70_09165"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8116570.1"
                     /translation="NPRRTPSLTHGSNTFGCDAPRLQTVEVYDSEGRVKKVPPCFDFL
                     TEGDLLNRARIPWAQYAASEDQKGYIWSAYSAIRRYRMHEDRWQRHMFPVDEVVRDIR
                     DGLLPPVTWITPRFELSEHPEFNFCHGENWSTKVIDAIMRSPMWDSTAIFLTWDDYGG
                     FYDHVPPPQVDDFGFGIRVPMMVLSPYAKHGKVSGELGEFSSVLRFIEDNWGLTQLTK
                     RDKQATPMLSAFDFDQEPRAPDPLPLRDDCMGPIWAPND"
BASE COUNT          272 a          621 c          597 g          302 t
ORIGIN      
        1 cttgcgttcg tgcgggacga ggtaggcggg gaacttcagc gcgatcacca tgtcgatcgg
       61 gagcccgttg gattcggaca ggtcgagcgc gcgccacacc gccatctggt gcacgatctc
      121 ggtcgccgga taccacttgc cggcgaccgc gacctcctcg gcctcgtgcc ccgcctcgcg
      181 cagggcgccg acgaccgact cggtgtgcgt ctcggctccc ccgcgcacga aaggggtctg
      241 gggatggacg accgcgatcc tcactcgacg tccccgagct tcgaacgcag ctccttcacc
      301 tcggtctcga gcgcgacgac ccgatcggcg agcgcggcgg cgagctcgcc gaactcggcc
      361 agcagccggt cgttgcggcg gcgcatgggg cggaggagac ggaactggag caccttggtc
      421 ttccgccgca tgcgggagcc ggatccgagc tcgggctcgc cgcgcgggac cgcctgtttc
      481 tcgtcggccc gcatgagcat cgcgaagtcg tggagcaggt cgcgcacctc ggaggtatcc
      541 atccgagacc gaaccgaggc gtcctcggcc cagtagatct cgcgttcgag cgcgctccga
      601 gcgtcgttct cgctggtcac gggcgtgagg gtagcaccgc ctccgatgtc atcagcccga
      661 tcaccatgac ctcgggacgc tcggcgaggc ctagcgctcc cgagtgacct cgaagtgctt
      721 gaccctcggt tcgaactcga gcaggaagcg ttcgtcctcg gggtagtagt gggcagcgtc
      781 cacgtcctgg ccggcgaacg ccttcacgtc gtcgagcgag tcccacaggg acatcacacc
      841 gaactccgcc tcgtcaccgt cgagacggcg gagcaccatc accccacggt tgccgggcgt
      901 ggcgtgaagc tccgcgaccc cgctctcctc caggtacgcc agatacccgt cgtagtccgc
      961 cgccttcgtg cgtccgaacc acatccgtgc gatcacggtc cgcctccttc gctcggtcta
     1021 gtcgttcggc gcccagatcg ggcccatgca gtcgtcgcgg agcggcaagg ggtccggcgc
     1081 ccgcggctcc tggtcgaagt cgaatgccga gagcatcggc gtcgcctgct tgtcgcgctt
     1141 ggtgagctgc gtgaggcccc agttgtcctc gatgaaccgg agcacgctgg agaactcgcc
     1201 gagctcgccg ctcaccttgc catgcttggc gtacgggctg agcaccatca tcgggacccg
     1261 gatcccgaac ccgaagtcgt cgacctgcgg cggcggcacg tggtcgtaga agccgccgta
     1321 gtcgtcccac gtgaggaaga tcgcggttga gtcccacatc ggcgatcgca tgatcgcgtc
     1381 gatcaccttc gtcgaccagt tctcgccgtg gcagaagttg aactcggggt gctcggacag
     1441 ctcgaagcgc ggcgtgatcc aggtgaccgg cggcagcagg ccgtcacgga tgtcccgaac
     1501 gacctcgtcg acgggaaaca tgtgccgctg ccagcggtcc tcgtgcatcc ggtagcgacg
     1561 gatcgctgag tacgccgacc agatgtagcc cttctggtcc tcactcgccg cgtattgggc
     1621 ccacgggatc cgcgcgcggt tcagcaggtc gccctcggtg aggaagtcga agcacggggg
     1681 caccttcttc acccgcccct cgctgtcgta gacctcgacg gtctgcagcc gcggcgcgtc
     1741 gcagccgaac gtgttcgagc cgtgggtgag gctcggcgtc cgtcgcgggt tg
//
LOCUS       DATBRK010000305         7864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_486828, whole genome shotgun sequence.
ACCESSION   DATBRK010000305 DATBRK010000000
VERSION     DATBRK010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486828"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VIH70_09170"
     CDS             complement(<1..678)
                     /locus_tag="VIH70_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY8116571.1"
                     /translation="MPPRAMWKGAISFGLVTIPVAVYPATEEKSLKFNQLHDEDMGRI
                     RYKRVCSVDGEEVEFDHIVKGYEVEKDKYVVMTDEDFDAVPVESSRAIDIQQFVDLEE
                     IDPILFKKSYYLVPDETGAKAYALLRKALSDERKVGIAKVSFRDKEHLSALRFKDDVF
                     VLETMYWPDEVRAAEFDTVPADEKVRENEVDMARSLIESLTEPWNPEAFTDAYREAML
                     EIVEKKLA"
     gene            801..1361
                     /locus_tag="VIH70_09175"
     CDS             801..1361
                     /locus_tag="VIH70_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HEY8116572.1"
                     /translation="MLRARAFALATLLFAACSTGETQVEGGGTPATGPTASGPTATGP
                     TATGPTGETAAGEFDGPVSVDTQAQSVAGLTAAFYSCDGVNGVWTYTVQGGPFDFDVD
                     STVDMEGGTGTLVISEDFTVEPLGNISFTDTIDLVIAGTADAPVFRATSIDVKIDSAA
                     PGLDQIAKSFFKKNTEVPILAGAKQC"
     gene            1389..3887
                     /gene="ligD"
                     /locus_tag="VIH70_09180"
     CDS             1389..3887
                     /gene="ligD"
                     /locus_tag="VIH70_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY8116573.1"
                     /translation="MSPRKKTSGGRAFTVEFPRPLPVEKRGDAWWMEADGRELRLSNL
                     DKVFWPDEGYTKGDLVAFYYNVAGLILPHLHDRPLTMKRMPDGVNGPFFYEKTAPSHT
                     PDWIARCPVQSEDSKDGVIGYMMVQDIATLLFVVNLGAIEMHPLHSRCPTIESPDYLF
                     FDLDPMGASFEDVLIVARHVRAALGALGLTGYPKTSGATGVQIYVPIRLGWSYDQVRN
                     FVGTLGRTIERADREHVTMAWQISNRTGKVFIDHNMNRSGANISAAYSLRPEAMATVS
                     TPVTWDEIEAGITPQDFRIENVWERFDKLGDLFEGVRTQPQELSPALEAVGMPAEKAF
                     ADPSQATRRTSEEVIAASKDPKLGEYLRKRTFGPEGTTEPAPGDASGAGNTFVIHKHR
                     ATRLHYDVRLERDGALPSWAVPRGLPTQPGDKRLAVRTEDHPLEYGSFEGTIPEGHYG
                     AGEVRIFDDGTYEPVEWTDSKVSFRLHGRRYPGLEFHFVKTRTEWLAFLASRQVSPLI
                     SSPPAFLPMLAEGGWKAFDDPKWWFEPKFDGIRSVTRMATDATHLVTRNGRDVTEKYP
                     ELRMIHELVDQVNAVLDAEIVAFDEDGKNSFEALQQRMNLSNERDIKRISSKIPVALV
                     AFDLLWLDGNDLTELPLQERRELLETIVEQDHRLQVVTHVEGGGKDFEKVARKLGLEG
                     VMAKRVGSRYQPGRRSPDWRKIKLTNTQDCVILGWTPGQGGRSGTFGALLVGAYDDGH
                     LLWIGQVGTGFTRQTLDRVLEALEPLRRPTPPIEDPELARVRGATFVEPEVVCEVEYL
                     EITKSTKKMRAPSFKGLREDKTPEESVLELPRGR"
     gene            complement(3895..4410)
                     /locus_tag="VIH70_09185"
     CDS             complement(3895..4410)
                     /locus_tag="VIH70_09185"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HEY8116574.1"
                     /translation="MEEPLGDDERETFGLEPEEAQNIRADLEDLEGMRRTFQGQGVKG
                     VVIACERCEENHFYEWDLLLENLDHMLQTGESQMHEPAFEVREEEYIQWDYGKGYVDA
                     LADTGLEPDNRIEVTRCPWCETPAEDHFQFCPKCGRSFAAVRIYKELVGRGLDEREVR
                     AMLVRAGFEPF"
     gene            complement(4410..5129)
                     /locus_tag="VIH70_09190"
     CDS             complement(4410..5129)
                     /locus_tag="VIH70_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY8116575.1"
                     /translation="MGFASALVIFAHPDDAEFMCGGTLASWVNEGTDVNYCVVTDGSA
                     GSNEPGMTREKMVPIREREQRAAAEVLGVRSVSFLGFVDGELQVDLATRKAVTRVVRR
                     VRPEVIVAPDPSRLWFGNRYINHWDHKQAGTLALCAVMPDAPSRPQFPELLDEGLEPF
                     EVPNLWLASDDADTYVDISKTLETKLESLRAHESQGTGDALDWVRERARELGEEGGVE
                     YAEGFKTFDLRDEDLDVTEPV"
     gene            5216..6328
                     /locus_tag="VIH70_09195"
     CDS             5216..6328
                     /locus_tag="VIH70_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116576.1"
                     /translation="MDDPLERDRRIRELERRFRSTGLPLLAEDYSATEDVFNRAIPLL
                     AVVFVLEILGAINLDWPPALNVLALLGGVALVLGGFGLLNVSRGRRFFSVPSKLGVPE
                     LTAFVVLPVVLPLVFGGQLTSALVTGLGNLLLLGLIWLVVGYGVFSILRWIAARFVQL
                     LGASLTLLVRALPLLLFFSLVTFFTNEYWQLFGEASDVTFYAGAGLFALITAVFLIVR
                     LPGSVRDLERGSSLAVPLHRRQRVNVGLVIFLAQALQIAFVGVAIWLFFMVFGALLVT
                     PAIRTDWIGTEGHVLFTLPFFGEQLAITKQLVRTAAGVASFSALYYAVASAVDSTYRD
                     EFIERLTQQLRETFRERGEYLELIAARDRAVASHLG"
     gene            complement(6325..7290)
                     /locus_tag="VIH70_09200"
     CDS             complement(6325..7290)
                     /locus_tag="VIH70_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8116577.1"
                     /translation="MGRRRGGRIQAEMSALTLAVVGDTLFGTDVDERRSATVRRALTD
                     TLGMFDRVYSPLFRLLVRLPSPTMRRYRRVESDLNRVIAEMVAERRAAGASGDDLLSL
                     LLRAGEDGAGMTDGQVRDEALTLFLAGHETTAIALTWTWWLLSRNPEAEALLHAELDV
                     VLPDRAPSVGDLPSLPYTQAVISESIRLRPPAWAIGRTAIAGHRANGYAIDRGSIVVV
                     SPWLLHHDLRWWPGPESFRPERWLDDDPERPRYAFIPFGGGPRVCIGEPFARLEAAML
                     LAAIARRWRFASRIDREPGLQAVITLRPRGGLPMVALRRRTPDGS"
BASE COUNT         1198 a         2729 c         2689 g         1248 t
ORIGIN      
        1 ggcgagcttc ttctcgacga tctcgagcat cgcctcgcgg taggcgtccg tgaaggcctc
       61 ggggttccag ggctcggtca ggctctcgat cagcgaccgg gccatgtcga cctcgttctc
      121 gcggaccttc tcgtcggccg gcacggtgtc gaactcggcc gcccggacct cgtcgggcca
      181 gtacatcgtc tcgaggacga acacgtcgtc cttgaaccgg agcgcggaca ggtgctcctt
      241 gtcgcggaag ctgaccttcg cgatccccac cttgcgctcg tccgagagcg ccttgcgcag
      301 aagcgcgtag gccttggccc cggtctcgtc cgggacgagg tagtaggact tcttgaacag
      361 gatcgggtcg atctcctcga ggtcgacgaa ctgctggatg tcgatcgcgc ggctcgactc
      421 gaccgggacc gcgtcgaagt cctcgtcggt catgaccacg tacttgtcct tctcgacctc
      481 gtaccccttg acgatgtggt cgaactcgac ctcctcgccg tcgaccgagc acacgcgctt
      541 gtatcggatc cggcccatgt cctcgtcgtg cagctggttg aacttcagcg acttctcctc
      601 ggtggccggg tagacggcga ccgggatcgt gacgaggccg aacgagatgg cccccttcca
      661 catcgctctg ggtggcatgg acgctcctcg ctgtgtggac ggcaggagcc tgggattcta
      721 atcggccgga gggacaaatc ccccccccgg cggccccgcc ggcttgaccc cgttgacggc
      781 gcaggtatac gaactcgttc gtgctccgag cccgcgcgtt cgcgctcgcg accctgctct
      841 tcgccgcgtg ctcgacgggc gagacccagg tcgagggtgg aggcacgccg gcgaccggcc
      901 cgacggcgtc ggggccgacg gcgaccggac cgacggcgac cgggccgacc ggcgagaccg
      961 ccgccgggga gttcgacggc cccgtctcgg tcgacaccca ggcccaatcc gtggcggggt
     1021 tgacggcggc cttctactcc tgcgacgggg tgaacggtgt gtggacctac acggtgcaag
     1081 gcggaccctt cgacttcgac gtcgactcga ccgtggacat ggagggcggc acgggaaccc
     1141 tggtcatcag cgaggacttc accgtcgaac ccctgggaaa catctcgttc accgacacca
     1201 tcgatctcgt gatcgcgggg acggccgacg cgcccgtctt cagggccacc tcgatcgacg
     1261 tgaagatcga ttcggcggcc cccgggctcg atcagatcgc gaagtccttc ttcaagaaga
     1321 acaccgaggt cccgatcctg gcgggcgcga agcagtgctg agtccggcct gaccgatacg
     1381 cttgcggcat gtccccgcgc aagaagacct cgggcggccg cgcgttcacc gtcgagttcc
     1441 ccaggccgct cccggtcgag aagcgcggcg acgcatggtg gatggaggcc gacggccgcg
     1501 agctgcgcct gtcgaacctc gacaaggtgt tctggcccga tgagggctac accaagggcg
     1561 acctggtcgc gttctactac aacgtcgccg ggttgatcct gccgcacctc catgaccggc
     1621 cgctcacgat gaagcgcatg cccgacggcg tgaacgggcc cttcttctac gagaaaacgg
     1681 cgccgtcgca cacgcccgac tggatcgcgc gatgtcccgt ccagagcgag gacagcaagg
     1741 acggcgtgat cggctacatg atggtccagg acatcgcgac gctgttgttc gtcgtgaatc
     1801 tcggcgcgat cgagatgcac ccgctgcact cgcgctgccc gacgatcgag tcgccggact
     1861 acctgttctt cgacctggac ccgatgggtg cgagcttcga agacgtgctg atcgtcgcgc
     1921 ggcacgtccg ggcggcgctg ggcgccctgg ggttgaccgg ctacccgaag acgtccggcg
     1981 cgaccggcgt ccagatctac gtgccgatcc ggctcggctg gtcctacgac caggtgcgta
     2041 acttcgtcgg cacgctcggg cggacgatcg agcgcgccga tcgcgagcac gtgacgatgg
     2101 cgtggcagat ctccaatcgc accggcaagg tcttcatcga tcacaacatg aaccggagtg
     2161 gcgcgaacat ctcggccgcc tactcgctgc ggcccgaggc gatggccacg gtctcgacac
     2221 cggtgacgtg ggacgagatc gaggctggga tcacgccgca ggacttccgg atcgagaacg
     2281 tgtgggagcg gttcgacaag ctcggcgacc tgttcgaggg cgtgcggacg caaccacagg
     2341 aactgtctcc ggcgctcgag gcagtgggga tgccggccga gaaggcgttc gcggacccga
     2401 gccaggcgac ccggcgcacg tccgaggagg tgatcgccgc gtcgaaggac ccaaagctcg
     2461 gcgaatacct gcgcaagcgc accttcgggc cggaggggac caccgagccg gcgcccggcg
     2521 acgcgagcgg cgcgggcaac acgttcgtga tccacaagca ccgcgcgacg cgcctgcact
     2581 acgacgtcag gctcgaacgc gacggggcgc tgccgtcctg ggcggtgccc cgcgggctgc
     2641 cgacccagcc gggcgacaag cggctcgccg tccgcaccga ggatcacccg ctcgagtacg
     2701 gctcgttcga gggcacgatc cccgaggggc actacggggc gggggaggtg aggatcttcg
     2761 acgacgggac gtacgagccg gtcgagtgga cggactcgaa ggtgagcttc cggctccacg
     2821 gccgccggta cccgggcctg gaattccatt tcgtgaagac gcgcaccgaa tggctcgcct
     2881 tcctggcgtc ccggcaggtg tcgccgctga tctcctcgcc gcccgcgttc ctgccgatgc
     2941 tcgccgaggg cgggtggaag gcgttcgacg acccgaagtg gtggttcgag ccgaagttcg
     3001 acgggatccg ttcggtgacg cggatggcga ccgacgccac gcacctggtg acgcgcaacg
     3061 gacgggacgt caccgaaaag tatccggagc tgcggatgat ccacgagctc gtggaccagg
     3121 tgaacgcggt gctcgacgcg gagatcgtgg cgttcgacga ggacgggaag aactcgttcg
     3181 aggcgctgca gcagcggatg aacctgtcca acgagcgcga tatcaaacgg atctcgagca
     3241 agatcccggt cgcgctggtc gccttcgacc tgctctggct cgacggcaac gacctgacgg
     3301 agctgccgct tcaggagcgg cgcgagctcc tggagacgat cgtcgagcag gaccaccggc
     3361 ttcaagtggt cacccacgtc gagggcggcg gcaaggactt cgagaaggtc gcgaggaagc
     3421 tcggcctcga gggcgtcatg gccaagcgtg tgggttcgcg ctaccagccg ggccgccgat
     3481 cgcccgactg gcggaagatc aagctcacga acacgcagga ctgcgtgatc ctgggttgga
     3541 cgcccggtca gggcggcagg tcgggcacct tcggcgcgtt gctcgtgggc gcctacgacg
     3601 atgggcacct gctgtggatc gggcaggtcg gcacggggtt cacgcgccag accctcgacc
     3661 gcgtgctcga ggcgctcgag cctctgaggc ggccgacgcc gccgatcgag gaccccgagc
     3721 tcgccagggt caggggtgcg acgttcgtcg agcccgaggt ggtctgcgag gtcgagtacc
     3781 tcgagatcac caagagcacg aagaagatgc gcgcgccctc gttcaagggt cttcgggagg
     3841 acaagacccc cgaggaaagc gtcctcgagc tccctcgtgg gcgctgatcg gtcgctagaa
     3901 gggttcgaac cccgcccgca cgagcatcgc gcggacctcg cgctcgtcca ggccgcgccc
     3961 gaccagttcc ttgtagatgc ggaccgccgc gaacgagcgg ccgcatttcg ggcagaactg
     4021 gaagtggtcc tcggcgggcg tctcgcacca cgggcaccgc gtcacctcga tgcggttgtc
     4081 gggctcgagc ccggtgtccg cgagcgcgtc cacgtagccc ttgccgtagt cccactggat
     4141 gtactcctcc tcgcggacct cgaacgccgg ctcgtgcatc tgggactccc cggtctggag
     4201 catgtggtcg aggttctcca gcagcaggtc ccactcgtaa aagtggttct cctcgcaccg
     4261 ctcgcaggcg atcacgacgc ccttgacgcc ctggccctgg aacgtccgac gcatcccctc
     4321 gaggtcctcg aggtcggcgc ggatgttctg cgcctcctcg ggttccagcc cgaaggtctc
     4381 gcgttcgtcg tcgccgagcg gctcttccat cacaccggct ccgtcacgtc caggtcctcg
     4441 tcccgcaggt cgaacgtctt gaacccctcc gcgtactcga ccccaccctc ttcgccgagc
     4501 tcgcgggccc gctcgcgcac ccaatcgagg gcgtccccgg ttccctgcga ctcgtgcgca
     4561 cgaagcgact cgagcttggt ctcgagggtc ttcgagatgt ccacgtacgt gtcggcatca
     4621 tccgaggcga gccacaggtt cggcacctcg aagggctcga gaccctcgtc gagcagctct
     4681 gggaactgcg ggcggctcgg cgcgtcgggc atgacggcgc acagcgcgag cgtccccgcc
     4741 tgcttgtggt cccagtggtt gatgtaccga ttgccgaacc acagtcgcga tgggtcgggc
     4801 gcgacgatca cctccggccg gacccgtcga acgacccgag tcacggcctt gcgggtcgcg
     4861 aggtcgacct ggagctcgcc atcgacgaac cccaggaacg acacgctccg cacgccgagc
     4921 acttccgcgg ccgcccgctg ctcacgttcg cggatcggca ccatcttctc gcgcgtcatc
     4981 cccggttcgt tcgagccggc cgagccgtcg gtgaccacgc agtagttcac gtcggtcccc
     5041 tcgttcaccc agcttgcgag ggtgccgccg cacatgaact cggcatcgtc gggatgggcg
     5101 aagatcacga gcgcgctggc gaaccccact cggaacagcg taccggctgc cgtcgtggtt
     5161 gcgagccgcc tcgaggcgct tgcgggtggg tgggcggcgg ggttcgatag cggacgtgga
     5221 cgacccgctc gaacgcgacc gccggatccg cgagctcgag cgccgcttcc ggagcacggg
     5281 gctgcccctg ttggccgagg actactcggc gaccgaggac gtcttcaacc gggcgatccc
     5341 gctgctcgcg gtggtcttcg tgctcgagat cctcggcgcg atcaacctgg actggccgcc
     5401 ggccctgaac gtcctcgcgc tgctcggcgg tgtcgcgttg gtcctcggcg ggttcggcct
     5461 gctgaacgtc tcgcgcggcc gcaggttctt ctcggtcccg agcaagctgg gggtccccga
     5521 gctcacggcg ttcgtcgtcc tgcccgtggt gctcccgctc gtcttcggcg ggcagctcac
     5581 gagcgcgctc gtcaccgggc tcggcaacct cctgctgctc ggcctgatct ggctcgtcgt
     5641 cgggtacggg gtcttctcga tcctgcggtg gatcgccgca cgattcgtgc agctgctcgg
     5701 cgcctcgctc accctgctcg tccgcgcgct cccgctcctg ctgttcttct cgctcgtcac
     5761 gttcttcacg aacgagtact ggcagctgtt cggcgaggcg agcgacgtga ccttctacgc
     5821 gggcgccggg ctgttcgcgc tgatcaccgc ggtcttcctg atcgtccggc tcccggggag
     5881 cgtccgagac ctcgagcggg gcagctccct cgcggtcccg ctccaccggc ggcagcgcgt
     5941 caacgtcggg ctggtgatct tcctcgcgca ggctctgcag atcgcgttcg tcggcgtcgc
     6001 gatctggctg ttcttcatgg tgttcggcgc gctcctggtc acgccggcga tccggacgga
     6061 ctggatcggc accgaggggc acgtcctgtt caccctgccg ttcttcggcg agcagctcgc
     6121 gatcaccaag cagctcgtcc gcacggccgc cggcgtcgcg tcgttctcgg cgctgtacta
     6181 cgccgtcgcc tcggccgtcg actccaccta cagggacgag ttcatcgagc gtctcacgca
     6241 gcagctgcgg gagacgttcc gcgagcgggg cgagtacctc gagctgatcg cggcccggga
     6301 ccgggccgtc gcgtcccacc tcggctagga cccgtcgggc gttcggcggc ggagcgcgac
     6361 catcggcagc cccccgcggg gtcgcagggt gatgacggcc tggagacccg gctcgcggtc
     6421 gatccggctc gcgaaccgcc accgccgcgc gatcgccgcg aggagcatcg ccgcctccag
     6481 ccgcgcgaac ggctcgccga tgcacacgcg cggacctccg ccgaacggga tgaacgcgta
     6541 ccgtggtcgc tcggggtcgt cgtcgagcca gcgctcgggg cggaacgact cggggccggg
     6601 ccaccatcgg aggtcgtggt gcaggagcca cggcgagacc acgacgatcg agccccggtc
     6661 gatcgcgtag ccgttcgccc ggtggccggc gatggcggtc cggccgatcg cccaggccgg
     6721 cggccggagc cggatcgact ccgagatcac ggcctgcgtg tacggaaggc tcggcagatc
     6781 gccgaccgaa ggcgcgcggt ccgggaggac cacgtcgagc tcggcgtgca ggagcgcctc
     6841 cgcctccggg ttccgcgaaa gcagccacca cgtccaggtg agtgcgatcg cggtggtctc
     6901 gtgaccggcg aggaacagcg tgagggcctc gtcccgaacc tgcccgtcgg tcatgccggc
     6961 cccatcctca cccgcgcgga gcagcagcga gaggagatcg tccccgctcg cgccggcggc
     7021 ccgccgttcc gcgaccatct cggcgatcac gcggttcagg tcggactcga cgcggcggta
     7081 gcgacgcatc gtcggcgagg gcagccgcac gagcaggcgg aacagcggcg agtacacgcg
     7141 gtcgaacatc ccgagcgtgt ccgtaagcgc ccggcggacg gtcgcgcttc gccgctcgtc
     7201 gacgtccgtc ccgaacaagg tgtcgcccac cacggcgagc gtgagcgcgg acatctccgc
     7261 ctggatgcga cctcctcgcc gtcgccccag cgctcggcgg cgcgctcggc gaacccgacc
     7321 atcgacgccg cgtaggtcgc gatccgctcg tggtggaaca tgggttggat cagtcgccgc
     7381 cgccggaggt gggtctcgtc ctcgctggtg agcagcgact cgcccagcag catcttcgcc
     7441 gcctggatcg tcggtccctt gtggaacgaa cgatgacccg tgaccaggac gtcgcggacg
     7501 aggtcgggat ggttcagcag gtacgcggcc gaacccggta catcgatccg aacgacgtcg
     7561 ccgtactcgg tcaggcgttc gaggagtccc accggatcgc tccgaaacgc ccggagcgcg
     7621 gcgagccggg acaggtcggg acccggcggt cgcacggtcg tcgtcacgga cccatcatcg
     7681 gcgacgcggg agtgaccgca acccgtaggc ttcgaacatg cgacccctgc ccgatggcta
     7741 cgaactacgg ccagccatgc tcgatgacct cgacggcgtg gccgcgacgc tgctcgcgga
     7801 cgacctcgcc gatgtcggcg agtcaggatc gaacccgagc gcgacctccc gcggatccac
     7861 gcga
//
LOCUS       DATBRK010000306         4268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_915172, whole genome shotgun sequence.
ACCESSION   DATBRK010000306 DATBRK010000000
VERSION     DATBRK010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915172"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VIH70_09205"
     CDS             <1..585
                     /locus_tag="VIH70_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEY8116578.1"
                     /translation="RVWGLRATDLNQGVVYGIETDETRLDERLLTRFDYDEVFGTALN
                     RFCLQAVIGQPVTPYGKGRQTRGYLNIVDTLQCVELAVLNPAVAGEFRVFNQFTEQFS
                     VVELAELVRHAGSEVGLDVPIEHVDNPRVELEDHYYNAKHIKLLELGLQPHLLSETLI
                     GSMFDVIGRYKDRLITDHILPATRWRGVRERSPG"
     gene            621..1667
                     /locus_tag="VIH70_09210"
     CDS             621..1667
                     /locus_tag="VIH70_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 family protein"
                     /protein_id="HEY8116579.1"
                     /translation="MWLLRVLGAFFAVGFFALVVVRYERKQVSRLTLIISSVISVAIL
                     LLAIAPGLFNPLFETFNFQPGTGLRLTGVLLAAVVILFLLVVRLQSNVDTNDRSIRLL
                     VESMGQTAFDWDAAAELPDGRRIVVISPALNEAENVGAVIHAMPSEVDGHAVVPIVID
                     DASDDGTADVARKAGALVASLPIRRGGGLALRVGYDVALKLDADVVVTIDADGQHQPE
                     EIPILVKPILDGEADHVNGSRMIGDFERESLIRHLGVHFFSRVVTILTGQRVTDISSG
                     YRATRADTLRRLLLEQDQFWTSEVTIEALRNHARVVEVPVTFLTRRGGESKKPKSLRY
                     GWNFSKAIVKTWLR"
     gene            complement(1648..2646)
                     /locus_tag="VIH70_09215"
     CDS             complement(1648..2646)
                     /locus_tag="VIH70_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006135299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEY8116580.1"
                     /translation="MPGGRSRRRELRDLARTLSVGRGGGDPGEAAALSVVRPSNEVAG
                     ALAAGEGVVALETSVVAQGLPPPRNLECAERMSAAIRSAGAVPAWIGVVRGEVVVGLS
                     VEELRVFAEPGGATKVARRDLPVAVASGGLGATTVSSTIWAAHRAGIVVGATGGIGGV
                     HPGSGDVSADLIELARTPVLLVCSGPKSIVDPVATAERLEELGVGVVGYACWRLPFFL
                     AREAKVDLDDRVNEPEDAARIARALGGLGAEAAVLLCNPVPEAFAMDAGVVEDAVRAC
                     ERIAEREGVRGKAITPFLLSCLSERTDGESLEANLALLESNARLAGEVAAASAARS"
     gene            complement(2547..3452)
                     /locus_tag="VIH70_09220"
     CDS             complement(2547..3452)
                     /locus_tag="VIH70_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY8116581.1"
                     /translation="MDVVTVGDVMIDVRVDSDGLEEGGDVHGRVLVRPGGSAANAAVW
                     AVEAGAKSRVHGRIGSDVAGALLRDELIARGVEPALAIDQTAETGTMLVVHEPRERSM
                     VADRGAGGRLSPDDLPERLEAGAVLVSGYSLLFEPTSAAGVAALERARARFVAVDAAS
                     WPMIRSFGAERFLQACAPATMLLANEREAEELTDRRGAAAFDELSRRFHVVCIKQGDE
                     GALMSWEGLVIRNATDAAIEKDPTGAGDAFNGVFLASLVAGRSPGDALSSACRAGARV
                     AASYETWPERSRSVEGEGTPGRRRR"
     gene            complement(3512..>4268)
                     /locus_tag="VIH70_09225"
     CDS             complement(3512..>4268)
                     /locus_tag="VIH70_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY8116582.1"
                     /translation="SLWDAFHRACEDIGLAVVTGHTARYDGCAWPMVGGAVCIAAGPE
                     DRFVTPTMARPGDRVIVTKGAAIEATALFAATFPERLTEGVGAGVVERADALFERMTV
                     VPEASVAREFGLRDQGVTSMHDATEGGVLGGLTEVASASEVGLRIELDAIPVREEVRA
                     VCEHIGIDPYTSISEGTLIATAVPDLAEAFVDALGTAGIESAIVGEVTEAADGVVLVT
                     GGGDRALEHPGLDPFWEAFGRWAAQAAGIAEAG"
BASE COUNT          636 a         1622 c         1411 g          599 t
ORIGIN      
        1 cgggtctggg gcctccgcgc caccgacctg aaccagggcg tcgtctacgg gatcgagacc
       61 gacgagacaa ggctcgacga gcgcctgctg acacgcttcg actacgacga ggtgttcggc
      121 acggcgctga accgcttctg cctgcaggcc gtgatcggcc agccggtcac cccgtacggc
      181 aaggggagac agacgcgcgg ctacctgaac atcgtcgaca ccctccagtg cgtggagctc
      241 gcggtcctga atcccgccgt ggcaggcgag ttccgcgtgt tcaaccagtt caccgagcag
      301 ttctcggtcg tcgagctcgc ggagctcgtc cggcacgcgg gttcggaggt ggggctcgac
      361 gtgccgatcg aacacgtcga caacccgcgc gtcgagctcg aggaccacta ctacaacgcg
      421 aagcacatca agctgctgga gctcggcctg cagccgcatc tgctctcgga gacgctgatc
      481 gggtcgatgt tcgacgtgat cgggcgctac aaggatcgcc tgatcacgga ccacatcctg
      541 cccgccacca ggtggcgggg cgtgcgggaa cgctcccccg gctgagccgg ccgattctac
      601 ccatcgcgta gagtcgggcc atgtggctgc tgcgcgtgct cggagccttc ttcgcggtcg
      661 gcttcttcgc gctcgtcgtc gtgcgttacg agcgcaagca ggtcagccgc ctcacgctga
      721 tcatctcctc ggtcatctcg gtcgcgatcc tgctgctcgc gatcgcgccg ggtctgttca
      781 acccgctgtt cgagacgttc aacttccagc ccggcaccgg cctgcgcctg accggcgtcc
      841 tgctcgcggc ggtcgtgatc ctgttcctcc tcgtcgtccg gctgcagtcg aacgtcgaca
      901 cgaacgatcg gtcgatccgc ctgctggtcg agtccatggg ccaaacagcc ttcgactggg
      961 acgcggcggc cgagctgccc gacggccgtc gaatcgtcgt catctctccg gcgctcaacg
     1021 aggccgagaa cgtgggagcg gtgatccacg ccatgccctc ggaggtcgac ggccatgctg
     1081 tggtgccgat cgtgatcgac gatgcgtccg acgacggcac ggccgacgtt gcgcggaagg
     1141 cgggcgcgtt ggtcgccagc ctgccgatcc ggcgcggagg ggggctcgcc ttgcgcgtcg
     1201 gctacgacgt cgccctcaag ctcgacgccg acgtggtggt cacgatcgat gccgacggcc
     1261 agcaccagcc cgaggagatc ccgatcctgg tcaagccgat cctggacggc gaggccgacc
     1321 acgtgaacgg gtcgaggatg atcggcgact tcgagcgcga gagcctgatc cgccacctcg
     1381 gcgtccactt cttctcacgc gtcgtgacga tcctcaccgg tcagcgcgtc accgacatct
     1441 cgagcggcta ccgcgcgacg cgcgcagaca cactccgtag gctcctcctc gagcaggatc
     1501 agttctggac ttccgaggtc acgatcgagg ccctccgcaa ccacgcgcgg gtcgtcgagg
     1561 tccccgtgac gttcctcacc agacgcggcg gcgagtccaa gaagcccaag tcgttgcggt
     1621 acggctggaa cttctcgaag gcgatcgtca agacctggct gcgctagccg ccgcgacctc
     1681 gccggccagg cgcgcgttcg actcgagcag cgccaggttc gcctccaggc tctcgccgtc
     1741 ggtgcgttcg gacaggcacg agagcaggaa cggcgtgatc gccttgccgc ggacgccctc
     1801 gcgttcggcg atccgctcgc atgccctgac cgcgtcctcg actaccccgg cgtccatcgc
     1861 gaacgcctcg gggaccgggt tgcacaggag caccgcggcc tcggcgccga gcccgccgag
     1921 cgcccgcgcg attcgggcag cgtcctccgg ctcgttcact cggtcgtcga ggtccacctt
     1981 ggcctcgcgc gcaaggaaga atggcagtcg ccagcaggcg tagcccacca cgccgacccc
     2041 gagctcctcg agccgctccg cggtcgcgac cgggtcgacg atcgatttcg gaccggagca
     2101 cacgagcaag accggggttc gggcgagctc gatcaggtcc gcggagacgt ccccgctccc
     2161 cggatgcacg ccaccgatgc cgccggtcgc gccgaccacg atccctgccc ggtgcgccgc
     2221 ccagatcgtg ctcgagaccg tggtggcgcc caggccaccc gaggcgaccg ccacggggag
     2281 atcgcgtcgg gccaccttgg tggcgccgcc gggctcggcg aacacgcgca gctcctccac
     2341 cgagagcccc accaccacct cgccccggac cacgccgatc cacgcgggga cggcgccggc
     2401 cgaacggatc gcggcgctca tccgctcggc gcactcgagg ttgcggggcg gcggaagccc
     2461 ctgggcgacg acgctcgtct cgagcgcgac cacgccctcc ccggcggcca gcgcgcccgc
     2521 gacctcgttc gaaggtcgga cgacgctcag cgccgccgcc tccccggggt cccctccccc
     2581 tcgaccgacc gagagcgttc gggccaggtc tcgtagctcg cggcgacgcg agcgcccgcc
     2641 cggcacgccg aggacagcgc atcgcccggc gaacgtccgg cgacgagcga agccaggaac
     2701 acgccgttga aggcgtcccc ggcgccggtg ggatccttct cgatcgcggc gtcggtcgca
     2761 ttgcggatca cgagcccctc ccacgacatc aacgcgccct cgtccccctg cttgatgcac
     2821 acgacgtgga agcgacgcga cagctcgtcg aacgcggcgg cgccgcgccg atccgtgagc
     2881 tcctccgcct cgcgttcgtt cgccagcagc atcgtcgcgg gcgcgcacgc ctggaggaac
     2941 cgctctgccc cgaacgagcg gatcatcggc cacgacgccg cgtcgaccgc gacgaaccgc
     3001 gcgcgggcgc gctcgagggc ggcaacgccg gccgccgagg tcggctcgaa caggagcgag
     3061 tacccggaca cgaggaccgc gccggcctcc aggcgttcgg gcaggtcgtc gggcgagagc
     3121 cggccgcccg cgccgcggtc cgcgaccatc gagcgctcgc gcggctcgtg gacgacgagc
     3181 atcgtcccgg tctcggcggt ctggtcgatc gccagagcag gttcgacacc gcgtgcgatc
     3241 aactcatcgc gaagcagcgc gccggcgacg tccgagccga tccggccgtg gactcgggac
     3301 tttgcgccgg cctccacggc ccagacggcg gcgttggcag cgctcccgcc gggccgaacg
     3361 agtacccgcc cgtgcacgtc gcccccctcc tccagtccgt cggagtcgac ccgcacgtcg
     3421 atcatcacgt cgccaaccgt gaccacgtcc atcgcgctgt gcagcctacc gggaacggtt
     3481 tcgttgatga gagccggtat gcggcgcggg ttcagccggc ctccgcaatc ccggccgcct
     3541 gcgcagccca ccgcccgaac gcctcccaga acgggtcgag ccccgggtgt tcgagcgcgc
     3601 ggtcgccgcc gccggtgacc aggacgaccc catcggccgc ctcggtcacc tcgccgacga
     3661 tcgcactctc gatccccgcg gtgccgaggg cgtcgacgaa cgcctccgcc aggtccggca
     3721 ccgccgtcgc gatcagggtg ccctcgctga tcgaggtgta cggatcgatc ccgatgtgct
     3781 cgcagacggc gcgcacctcc tcgcgcacag ggatcgcgtc gagctcgatc cgcaggccga
     3841 cctccgaagc cgaggcgacc tcggtcagcc cgcccagcac gccgccctcg gtggcgtcgt
     3901 gcatcgacgt caccccctgg tcccggagcc cgaactcccg cgcgacgctc gcctcgggca
     3961 ccacggtcat ccgctcgaac agcgcgtcgg cgcgttcgac cacccccgca ccgacgccct
     4021 cggtcaggcg ttcggggaag gtggccgcga acagcgccgt cgcctcgatc gcggccccct
     4081 tcgtgacgat cacccggtcg ccgggccggg ccatcgtcgg cgtgacgaac cgatcctcgg
     4141 ggcccgcggc gatgcagacg gcgccgccga ccatgggcca ggcacagccg tcgtaccgcg
     4201 ccgtgtgacc cgtcacgacg gccaacccga tgtcctcgca cgcgcgatgg aacgcatccc
     4261 agagcgag
//
LOCUS       DATBRK010000307         4171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_53727, whole genome shotgun sequence.
ACCESSION   DATBRK010000307 DATBRK010000000
VERSION     DATBRK010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53727"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VIH70_09230"
     CDS             <1..224
                     /locus_tag="VIH70_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116583.1"
                     /translation="VIASTVGDASEIFRRDACDTQIAISGGDLRIREAVFHAGDPHCC
                     PSAFRESTLRWNGTSWDEVSSEVVPNDGR"
     gene            421..3984
                     /locus_tag="VIH70_09235"
     CDS             421..3984
                     /locus_tag="VIH70_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116584.1"
                     /translation="MLALALAGAAALAYVQAGKVDERLSLETRRTAEHAAEAVASRLT
                     FEDIQQPMGEGRAGTISSFLERRLLDPSESVTIWSADAVIVFAADPALIGTQDRGLLT
                     RISRVLSDGTQTETAGGSVHTFVAVAVGEGEGRPVVAEVIRPEEASPAGHPWIYASIA
                     AAFLALLVLVAAVRTSGRQGYRPNGFGVDDLRRERKNLLQAQEALERSRTEEANLRSE
                     LERVAGDLALTKEALAAKETSIGEVDSRARAAEEQAARAEAELRAGVTVREEVARLID
                     TELQTARSELAMSKQQHEETQRQLESSRMAEAEREAAQAERDVLEAERDAIAAERDAL
                     RSLDERLRGELDAAKANLDIISREMTELGNRYQESAARAAHAEAKVLELNADRRELED
                     QRDPTGELQRTHAELEMTKNELGAALQKLETLGEQLVVSRGDLEAVRGEQELGNAELE
                     GARAELEGARAELDAVRAEFHAIRAELDAARADATSSSGVRDSTLFELDSIRTQQETT
                     TAALDETREELSASRAALEIARSNEERALAEAAEAHSSFEALSADLSNAREETEAAWA
                     EATALTSRMDEHVERAASAEARALELSQWVTELESRPDLTPALEDAKAALESAMDQLR
                     DRDARFAAARDELDSIRIQQERTTAALDEANKELSASEAALGSARLELETARSNERQA
                     LTEAAALARRVDEHVERAAHAEARESELWQRITELESRPDLTPALEDARAALESAKDQ
                     LGDRDTRFAAAQNELVAIQTSLQAATARADALEAEVERAEAKAAELSSEVGLQEARSA
                     ELAAEVERAEARAEAAEGRAIDLSGRIEEHETSLRELAGRAGRAEGLVTELAARAERA
                     EVRADEMAERAEAEIAAARERENPELEAARAEAEDLTGRLAVVTTELEAAAAFASALE
                     RRNEELEDQVASQAVSAEVDAADARARLIEAQQSLARLRETVEHHDELGSFVQEAPSM
                     APGTATEVADALSVNVKRSLSAIFELARTLSTDQQNEDDERFLYQLMAQAKGMENAIR
                     DIFDADRLTRGELVLERRNTEIDRLVDRVVREFPFAGDRDLDVAVEPATIMVDRVRVE
                     RLVDDLLNSAVGRTETGDRIALVLERAPDGVLISVEGGTPLNGDGTAGAAAAFLAGLH
                     GGWTKAERLSDGTGVVRAFLPSGPNAGTADGADPEAAALLG"
BASE COUNT          683 a         1432 c         1542 g          514 t
ORIGIN      
        1 gggtgatcgc ctccaccgta ggcgacgcga gcgagatctt ccgccgcgac gcgtgcgaca
       61 cccagatcgc gatctcgggc ggtgacctcc ggatccgcga ggccgtcttc cacgccggcg
      121 acccgcattg ctgcccgagc gcgttccgcg agtcgacctt gcgctggaac gggacctcgt
      181 gggacgaggt ctcgtccgag gtcgtcccga acgacgggcg ctgagcgacc gtttcggagg
      241 cccgccccta tcgcccggtc gcccgtccac cggtcgacgg accgctgcgg aactcgtgcg
      301 gcgaagggct tacgtcgacc caccggtcgg ccgatacagg gagcgatctc gccgatcgat
      361 ctacggaagg caggactgag cttgagcggt gccgttgggg cgcggagagt ggctttcgcg
      421 gtgctcgcac ttgccctcgc aggtgcggcc gcgctcgcgt acgtccaggc gggcaaggtg
      481 gacgagcgcc tgtcgctcga aacgcgtcgg acggccgaac atgccgccga ggcagtggcg
      541 tccaggctca cgttcgagga catccaacaa ccgatgggcg agggccgagc cggcacgatc
      601 tcctcgttcc tcgaacgccg gctgctcgac ccgagcgaat cggtcacgat ctggagcgcc
      661 gacgcggtca tcgtgttcgc ggcggacccg gccctgatcg gcacccagga tcgtggactc
      721 ctcacccgga tctcgcgtgt cctatcggac gggacccaga ccgagaccgc gggtggatcc
      781 gtgcacacct tcgtcgccgt cgcggtaggc gagggcgagg ggaggcccgt cgtggcggag
      841 gtcatccgcc cggaggaggc ctcgcccgcc ggtcatcctt ggatctacgc ctcgatcgcc
      901 gccgccttcc tcgcgctcct cgtgctggta gccgccgtcc gcacctccgg ccgtcagggc
      961 tatcgaccca acgggttcgg cgtggacgac cttcgccggg aacgcaagaa cctgctgcag
     1021 gcgcaggagg cgctggagcg ctcgcggacg gaggaggcga acctgaggag cgagctcgag
     1081 cgcgtggccg gcgacctcgc cctgaccaag gaagcgctcg ccgccaagga gacctcgatc
     1141 ggtgaggtcg actcgagggc ccgggccgcc gaggaacagg cggcgcgggc cgaggccgag
     1201 ctccgggccg gcgtgaccgt ccgcgaagag gtcgcccggc tgatcgacac cgagctccag
     1261 accgcgcggt cggagcttgc gatgagcaag cagcaacacg aggagacgca gcggcagctc
     1321 gagtcgagcc ggatggccga agccgagcgc gaagccgcgc aggccgagcg cgatgtcctc
     1381 gaggcggaac gcgacgcgat cgccgccgag cgtgacgcgc tccggagcct cgacgagcgc
     1441 ctgcgcgggg agctggacgc cgcgaaggcc aacctcgaca tcatcagccg cgagatgacg
     1501 gagctcggca accgctatca ggagtcggcg gctcgagccg cccacgccga ggccaaggtg
     1561 ctcgagctga acgccgatcg ccgcgagctc gaggaccaac gagatcccac gggcgagctc
     1621 cagcggaccc acgccgagct cgagatgacg aagaacgagc tgggcgccgc gctccagaag
     1681 ctggagacgc tcggggagca gctggtggtc tcccgcggcg acctggaggc cgttcgcggg
     1741 gagcaggagc tcggcaacgc ggagctcgag ggcgcgcggg cggagctcga gggcgcgcgc
     1801 gcggagctcg atgccgttcg agccgagttc catgccattc gagccgagct ggatgccgcg
     1861 cgcgccgacg cgacctcgtc gtcgggcgta cgcgactcga cgctgttcga gctcgactcg
     1921 atccgaaccc agcaggagac aaccacggcg gcgctcgacg agacccgcga ggagctgtcc
     1981 gcatcaaggg ccgcgctcga gatcgcacgg tcgaacgagg agcgagcgct ggccgaagcg
     2041 gccgaggcgc actcctcgtt cgaggccctc tcggcggatc tgtcgaatgc gcgcgaggag
     2101 accgaggcgg cgtgggccga ggcgacggcg ctcaccagcc ggatggacga acacgtcgag
     2161 cgcgccgcct ccgccgaggc aagggcgctg gagctgtcgc agtgggtcac cgagctcgag
     2221 tcccgaccgg acctcacccc cgcactcgag gacgcgaagg cggcgctcga gtcggcgatg
     2281 gatcaactga gggatcgcga cgcgcggttc gccgcggcgc gggacgaact cgactcgatc
     2341 cggatccagc aggagaggac cactgcggcg ctcgacgagg cgaacaagga gctctcggcg
     2401 tcggaggccg cgctcggatc ggcgcggctc gagctcgaga ccgcccggtc gaacgagcgg
     2461 caagcgctga ccgaggccgc cgcgctcgcc aggcgagtgg acgaacacgt cgagcgcgcc
     2521 gcccacgccg aggccagaga gtccgagctt tggcagcgga tcaccgagct cgagtcccgg
     2581 ccagacctca ccccggcgct cgaggacgcg agggcggcgc tcgagtcggc gaaggatcag
     2641 ctgggggatc gcgacacgcg gttcgccgcg gcccagaacg agctcgtcgc gatccagacg
     2701 tcgctccagg ccgccaccgc aagggccgac gcgctcgagg cggaggtcga acgggcggag
     2761 gccaaggcgg cggagctctc ctcggaggtc ggactgcagg aggccagaag cgcggagctc
     2821 gcggcggagg tcgagcgagc cgaggctcgg gccgaagcgg ccgagggccg ggcgatcgat
     2881 ctgtccggcc ggatcgagga acacgaaacc tcgttgcgtg agctcgccgg tcgggccgga
     2941 cgcgccgagg ggctcgtcac ggagcttgcg gcccgggcgg agcgagcgga ggtccgcgcg
     3001 gacgagatgg ccgagcgagc ggaggccgag atcgccgccg cccgcgagcg cgagaacccg
     3061 gagctcgagg ccgcgcgagc cgaggccgag gacctgacgg gcagactcgc cgtcgtcacg
     3121 accgagctcg aagccgcggc ggcgttcgcg agcgcgctcg agcgacgcaa cgaggagctc
     3181 gaggatcagg ttgcgtcgca ggccgtcagc gccgaggtcg acgccgccga cgctcgagcg
     3241 cggttgatcg aggcccagca atcgctcgcc cggctccggg agaccgtcga acatcacgat
     3301 gagctcgggt ccttcgttca ggaggccccg tcgatggccc ccggcaccgc caccgaggtc
     3361 gcggacgcgc tctcggtcaa cgtgaagcgt tcgctgtcgg cgatcttcga actcgcccga
     3421 accctgtcca ccgatcagca gaacgaggac gacgagcggt tcctctatca gctgatggcg
     3481 caggccaagg ggatggagaa cgcgatccgc gacatcttcg acgcggatcg gctcacgcgc
     3541 ggcgaactcg tgctcgagcg ccgcaacacc gagatcgaca gactggttga ccgtgtggtt
     3601 cgcgagtttc cgttcgccgg cgaccgcgac ctcgacgtcg cggtcgagcc ggcgacgatc
     3661 atggtcgacc gcgttcgcgt ggagcgcctg gtggacgatc tgctcaactc ggccgtcggg
     3721 cgcaccgaga ccggtgatcg catcgcgctc gtcctggaac gagcccccga cggtgtgctc
     3781 atctcggtgg agggcgggac ccctctgaac ggggacggca ccgccggggc ggccgctgcg
     3841 ttccttgccg ggctccatgg gggttggacc aaggccgagc gactctcgga cgggacgggc
     3901 gtggtccggg cgttcctgcc cagcggtccg aacgccggca cggccgacgg cgccgacccc
     3961 gaggccgccg cgctcctcgg ctgagttaaa cgtcgcctac accaggaccg ccgtgctccg
     4021 ccggcgcccg accttctgca cggagatcgt cgcggggaac tgatccttga tctgctcggc
     4081 gtgcgtgacc acgaacagct ggtggaaccg cgacgagagc cggccgagcg cctgcaccat
     4141 caggtccttg cgctcctcgt ccagcgaggc g
//
LOCUS       DATBRK010000308         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1063279, whole genome shotgun sequence.
ACCESSION   DATBRK010000308 DATBRK010000000
VERSION     DATBRK010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063279"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /locus_tag="VIH70_09240"
     CDS             complement(<1..1914)
                     /locus_tag="VIH70_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEY8116585.1"
                     /translation="MEHRAKTSRGDSFADVSKVHGRYRRPTGAPPPLPKKIGATGVLR
                     LVLVVLVVIPGCIWLHYNPAPLDRFDAAITDAIVSLRAGWLDTLARSVNTVASRYGLA
                     LLSLITVASVAWFRRWRHLTLFLIGVAVVGLSIEGLLLIAARPRPFDVTIIGAWEGYS
                     APSIPMAGFAVVIAGTAYTLVVPGRPRMYAKCLGGVVLILAALLRVYLGIDHFTDAWF
                     GVILGASIPVALFRAYAPNDVYPVRYGPQGKAAHLDVTGKRGEAIKLAMKEQLGYDVL
                     SMKPVGLEGSGGSTPLKMRIVDDQGVERSVFAKLYAKSHVRADRWYKLGRTMLYGRLE
                     DETPFSTVRRFVEYEDYTLRLLGEKGFSIPEPLDIVEITPEREYLIAMEFFENAVEIS
                     EAEIDDRIIDQGLQVIRRMWDVGLSHRDIKPANLMVQNGRLRLIDVFFVQVRPSPWRQ
                     AVDLGNMMLVLALRSDPQLVYERALAYFTPEELSEAFAATRGVASPSQLRASMKADGR
                     GLLETFRELAPPRKPIAIQRWSVRRVLLIVATVLLLLLTVLTGIALFIPSRGDVGNAT
                     CGTGRTMQLMAQAVPTATKLPCIEDLPLGWGTEGATVARDRATFSVGIGSDLLSPVVV
                     TLTETCPVDTSLQQ"
BASE COUNT          341 a          662 c          664 g          305 t
ORIGIN      
        1 ctgctggagg gaggtatcca ccgggcaggt ctcggtcagc gtcacgacca ccgggctcag
       61 cagatccgac ccgatcccga cgctgaaggt cgcccggtcc cgagcgaccg ttgccccctc
      121 cgtgccccag cccagcggta gatcttcgat gcacggcagc ttcgtggcag tcgggaccgc
      181 ctgtgccatc agctgcatgg tccggccggt gccgcacgtc gcgttcccca catcgccccg
      241 cgaggggatg aacaacgcga tccctgtcaa cacggtcaag agcaggagga gcaccgtggc
      301 aacgatcagc aggacgcggc ggacgctcca ccgctggatc gctatgggct tgcggggcgg
      361 cgcgagttcg cggaaggtct ccagcagccc gcgaccgtcc gccttcatcg atgcccgcag
      421 ctgcgacggg ctggcgacgc cccgggtcgc ggcgaacgcc tcggacaatt cctcgggtgt
      481 gaagtaggcg agcgcccgtt cgtagacgag ctgcggatcc gagcgcaggg cgagcaccag
      541 catcatgttg ccgaggtcga cggcctggcg ccacggcgac ggccgcacct gcacgaagaa
      601 cacgtcgatc aggcgcagcc gtccgttctg caccatcagg ttcgcgggct tgatgtcgcg
      661 gtgcgacagc ccgacatccc acatcctgcg gatcacctgc aacccctggt cgatgatccg
      721 atcatcgatc tcggcctcgc tgatctcgac ggcgttctcg aagaactcca tcgcgatgag
      781 gtactcgcgc tcgggcgtga tctcgacgat gtcgaggggc tccgggatcg aaaagccctt
      841 ctcgccgagg agtcgcaggg tgtagtcctc gtactccacg aagcgccgga ccgtgctgaa
      901 gggggtctcg tcctccaggc ggccgtaaag catcgtgcgc ccgagcttgt accaccggtc
      961 cgctcgcacg tggctcttcg cgtagagctt cgcgaagacc gaccgctcga caccctggtc
     1021 gtcgacgatc cgcatcttca ggggcgtgga gccgccggat ccctcgagcc cgacgggctt
     1081 catgctgagg acgtcatagc cgagctgctc cttcatggcg agtttgatcg cctcgccgcg
     1141 cttgccggtc acgtcgaggt gcgccgcctt gccctgaggg ccgtatcgca cggggtagac
     1201 gtcgttcggc gcgtacgcac ggaacagcgc aacagggatc gaggcgccga ggatcacgcc
     1261 gaaccatgcg tcggtgaaat ggtcgatccc gaggtagaca cgcagcaggg cggccaggat
     1321 cagcacgact ccgcccaggc acttggcgta catccgtggg cggcccggga ccacgagcgt
     1381 gtacgcagtg ccggcgatca cgaccgcgaa acctgccatt ggtatggagg gggccgaata
     1441 gccctcccag gcaccgatga tcgtgacgtc gaagggtcgt ggccgggccg cgatgagcaa
     1501 cagcccctcg atggaaagcc cgacgacggc cacgccgatc aggaacagtg tgagatgtcg
     1561 ccaccgccgg aaccacgcga cgctcgcgac ggtgatcagg ctgaggagcg cgagcccgta
     1621 gcgcgaggcg accgtgttga cggatcgggc cagggtgtcg agccaccccg cgcgcagcga
     1681 gacgatggca tcggtgatcg cggcgtcaaa ccggtcgagc ggcgccgggt tgtagtgcaa
     1741 ccagatgcac ccggggatga cgacgagcac gaccaacacc aatcggagga cgccggtcgc
     1801 gccgatcttc ttcggcagcg ggggcggcgc tccggttggg cgccggtagc gcccgtggac
     1861 ctttgacacg tcggcgaagc tgtcgcccct ggaggtcttt gcacggtgtt ccacggcttc
     1921 cgatcctccc ccagcgatcc tcgcacgatc gtcgccggcc ggtatagatc gt
//
LOCUS       DATBRK010000309         6559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1063677, whole genome shotgun sequence.
ACCESSION   DATBRK010000309 DATBRK010000000
VERSION     DATBRK010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063677"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..279)
                     /gene="rnpB"
                     /locus_tag="VIH70_09245"
     ncRNA           complement(12..279)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIH70_09245"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            345..1283
                     /locus_tag="VIH70_09250"
     CDS             345..1283
                     /locus_tag="VIH70_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116586.1"
                     /translation="MRARVALAIVPVIGSVLALAACAQEPVGPAAPIAPAQLAPGPAL
                     EASAARRAVVEFLEAYRESPDQGIGRLRQLVIGPDLRSWVRWLDVQHREFDGTIASAA
                     DVRDVEFIGAVTAHRIPLALVGLSASVRFRFDPPDDRPIDLVRVLDGPVTLVRTAAGT
                     YRVEDLLRNGVPMSDSIQPFRHQVRREGGVTVHLDSLFMFPPKWQFNVVIENASPYDV
                     SLDPDGSALFVDRGDGFERVEGVLTASLGSVPAGEAAEGLMVFQAQRSADGRVLSLVY
                     GRGPRALRFEFPLQDLVTAVPPPAPTDAGTAETAPS"
     gene            complement(1222..1713)
                     /locus_tag="VIH70_09255"
     CDS             complement(1222..1713)
                     /locus_tag="VIH70_09255"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEY8116587.1"
                     /translation="MPSDRRPRLILHCDGAARGNPGPAGIGVEITDEDGEVVAEIAEG
                     IGVATNNVAEYTAAIRGLERARELDATHVLLRSDSRLLIEQLAGRWKVKNPTLQRLHR
                     EARSLISEFEQVRFEHVRRERNVEADALANRGVDEWLAGDGSSWERSPPSPRLWEPGE
                     EPR"
     gene            complement(1686..2396)
                     /locus_tag="VIH70_09260"
     CDS             complement(1686..2396)
                     /locus_tag="VIH70_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEY8116588.1"
                     /translation="MRGLDRILELQDLDTAIDRLEHRREQLEAGEELSSLRKEMEEAD
                     ARLGELRLASDSISSDSTRLEHEIASMSDKLHAEEKRMYDGSIANAKELEALQHEIAS
                     LKERRSRAEDDLLEQMVRKEDVDARAAQAQTEAEAARARVEEVGGDAVRELDQISADL
                     ADKRSERGSLVPAFDEELLELYDDLREQKHGVGAAAIVDGVCQACHEKLSAVELERLK
                     RTEGVKRCEYCRRIVVLV"
     gene            complement(2426..3409)
                     /locus_tag="VIH70_09265"
     CDS             complement(2426..3409)
                     /locus_tag="VIH70_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY8116589.1"
                     /translation="MAPEPLFEIARRRFDTPEFRGMEFIEAECKTIINHVPGNYLPFN
                     WSINPYRGCSHACKFCFARPTHTFLNMNAGSDFEKKIVVKVNAPEVLRRQLAAKRWKG
                     EGIAMGTNTDPYQRAEGRYRLMPGIIRALTDYRNPFSILTKGTLILRDLELLVEAGRV
                     TDVSTAFSIGTLDEAAWRSSEPGTPPPRKRIDAVRALNEAGIPCGVLMAPILPGITDD
                     PRQLREVVAAAIDAGATHVSPILLHLRPGVREEFLPWLAETHPDLVERYEELYRKPYA
                     PTAERDALGRRVSGLIRSLGGVRRAPAPPARFSRGSRGDPGAAQGEQLTLL"
     gene            3486..4106
                     /locus_tag="VIH70_09270"
     CDS             3486..4106
                     /locus_tag="VIH70_09270"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8116590.1"
                     /translation="MAIWLVRHCETEWSISGRHTGSTDLPLTPEGRLQATAIGKLLAG
                     RLFDHVFSSPMLRARETARLAGFADRVRAHEGMLEYDYGEFEGLTTGQIWETHPGWQL
                     FRDGCPGGETPEQMAKRVDVLIEELRELGGNSLLFGHGHCFRALAVRFLDLPIRYATN
                     LLLDAGTVSITSDARDGPALVLWNRRVQPRAMIVADIAIAIGRTVP"
     gene            4129..4416
                     /locus_tag="VIH70_09275"
     CDS             4129..4416
                     /locus_tag="VIH70_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116591.1"
                     /translation="MGCLFAIVALITPRFILFFMWLFTNYLNTAFSSGWWGLLGFFFL
                     PTTTIAYAIAQNEFTTVTGGVEATGIVLIVLGIVIDLGLLGGSGRGVARRG"
     gene            4636..6192
                     /locus_tag="VIH70_09280"
     CDS             4636..6192
                     /locus_tag="VIH70_09280"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8116592.1"
                     /translation="MADGAAFEFEDASIDELQASMASGSVTSRTLTEAYLERIERVGA
                     ALRSVLETNPDALEIADTLDAERRSGRVRGPLHGVPVLVKDNIDTADRMNTTAGSFAL
                     GEATPARDAFLVGRLREEGAVILGKANLSEWANFRSTRSSSGWSARGGQCVNPYDPNR
                     SPCGSSSGSGVAVSANLSAVAVGTETDGSIVCPSSVNGIVGIKPTVGLVSRSGVIPVS
                     HSQDTPGPMARTVADAAALLTALAVADPADAATAGARVEPDYAAFVDPDGLRDARIGA
                     ARNLAGFNDRVDRLFDEALDAMRDLGAEIVDPAEIPHTDDLEDPELEVFLYEFKADLE
                     AYLGSYGPTQPHRTLADLIAYNEEHVDTEMPFFGQELFEQAAEKGPLTEPAYLEALAA
                     CRRLSRAEGIDAVMDEHRLDALVAPSNAPAWRIDYANGDHYVGGNSTPAAVAGYPSVT
                     VPMGFAFDLPIGISFIGRAWSEGTLIRLASAFEHATGHRRPPGLGRISSQDRRDRART
                     TGARPDAKAG"
     gene            6227..>6559
                     /locus_tag="VIH70_09285"
     CDS             6227..>6559
                     /locus_tag="VIH70_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8116593.1"
                     /translation="MEGSAPTTSSDRGLAREAIGLREVFFQSVTHMAPAAAVAFSIIV
                     GANFASGALPLSVIFALVGCLLVAISIGQLAKHLPSAGGFYTYTARGIHPSIGFLVAW
                     GYAFVEPLV"
BASE COUNT          922 a         2302 c         2256 g         1079 t
ORIGIN      
        1 gcgctctcgc gagcaccccg gggccgaagc cctcgtgcgg cctacccggg agctcggacg
       61 ggccgtcctc gaacgctccc tgcttggcct agctccgggt ggggcttgcc gagccgagcc
      121 ggtcacccgg cccgctggtg agctcttacc tcaccgtttc acccttaccg ctcacgcggc
      181 ggtctgttct ctgtggcgct ttccacgggt cgccccgcct gggtgttacc cagcaccctg
      241 ccctgcggag tccggacgtt cctcgagcgt tgccgcccgc ggccgcccgg ccggctcctc
      301 cgcgcccagt ctagagaaac gccaccggcc gtatcatccg cgacatgcgt gctcgcgtgg
      361 cgctcgcgat cgtgccggtg atcgggtcgg tgctcgcgct cgccgcatgc gcccaggaac
      421 cggtcggccc cgcggcgccg atcgcgcccg cgcagctcgc accgggtcct gccctcgagg
      481 cctcggccgc gcggcgggcg gtcgtcgagt tcctcgaggc gtaccgggaa tcgcccgatc
      541 agggcatcgg gcggctgagg cagctcgtga tcggccccga tctccggtcg tgggttcggt
      601 ggctcgacgt ccagcaccgc gagttcgacg ggacgatcgc gtccgccgcc gacgtgcgcg
      661 acgtcgagtt catcggggcc gtgacggccc accgcatccc cctcgcgttg gtcggactgt
      721 ccgcatcggt ccggttccgg ttcgaccccc ccgacgaccg cccgatcgat ctcgtccggg
      781 tcctggacgg ccctgtcacg ctggtccgaa cggccgccgg cacctaccgc gtcgaggacc
      841 tgctgcgtaa cggcgtcccg atgtccgaca gcatccagcc cttccggcac caggtgcgaa
      901 gggagggcgg cgtgacggtg cacctcgatt ctctcttcat gttcccgccc aaatggcagt
      961 tcaacgtggt gatcgagaac gccagcccat acgacgtctc gctcgatccc gacggatccg
     1021 ccctgttcgt cgaccgaggc gacgggttcg aacgcgtcga aggggtcctc acggcatcgc
     1081 tcggctcggt cccggcgggc gaggccgccg aggggctcat ggtcttccag gcgcagcgct
     1141 cggcggacgg gcgcgtgctc tcgctcgtct acggacgggg accgagggcg cttcggttcg
     1201 agttcccgct gcaggacctc gtcaccgcgg ttcctccccc ggctcccaca gacgcgggga
     1261 cggcggagac cgctcccagc tagaaccgtc gccggcgagc cactcgtcga cgccccggtt
     1321 cgcgagcgcg tccgcctcca cgttgcgttc gcgtcggacg tgctcgaacc gcacctgctc
     1381 gaactcgctg atcagtgagc gcgcctcccg gtggagccgc tgaagggtcg ggttcttcac
     1441 cttccatcga ccggcgagct gttcgatgag cagccggctg tccgagcgca ggagcacgtg
     1501 ggtcgcatcg agctcgcggg cgcgttcgag gccgcggatc gcagccgtgt actcggcgac
     1561 gttgttggtc gcgacgccga tcccctcggc gatctcggcg acgacctcgc cgtcctcgtc
     1621 ggtgatctcc acaccgatcc ctgcaggccc ggggttgccg cgggccgcgc cgtcgcagtg
     1681 caggatcaga cgaggacgac gatccgacgg cagtactcac acctcttcac gccctcggtg
     1741 cgcttcaggc gctcgagctc gacggccgag agcttctcgt ggcaggcctg gcacaccccg
     1801 tcgacgatcg cggcggcgcc gacgccgtgc ttctgttcgc gaagatcgtc gtagagctcg
     1861 agcagctcct cgtcgaaggc aggcacgagc gacccgcgtt cggatcgctt gtcggccagg
     1921 tcggccgaga tctggtcgag ctcgcggacc gcgtcgccgc cgacttcctc gacccgcgct
     1981 cgggcggcct cggcctccgt ctgcgcctgc gcggcgcggg catcgacgtc ctccttccgg
     2041 accatctgct cgagcaagtc gtcctccgcg cgcgagcgac gctccttcag gctcgcgatc
     2101 tcgtgctgca gcgcctcgag ctccttcgcg ttggcgatcg agccgtcgta catgcgcttc
     2161 tcctccgcgt ggagcttgtc gctcatcgag gcgatctcgt gctcgaggcg ggtcgagtcg
     2221 ctcgagatcg agtccgacgc caaccggagc tcgccgaggc gggcatcggc ctcctccatc
     2281 tccttgcgga gcgacgagag ctcctcgccg gcctcgagct gttcgcggcg gtgttccagg
     2341 cggtcgatcg ccgtgtcgag gtcctgcagc tcgaggatcc ggtccagtcc gcgcatcgga
     2401 gcgccagggt atccgatgcc gggcgctaca agagcgtgag ctgctccccc tgcgcggcgc
     2461 caggatcgcc gcgggaccct ctcgagaagc gcgccggcgg ggccggcgcc cgccggacgc
     2521 cgcccaggga tcggatcagc ccggagacgc gccggccgag cgcgtcgcgt tcggcggtgg
     2581 gggcgtaggg cttgcggtag agctcctcgt accgttcgac gaggtcggga tgggtctcgg
     2641 cgagccaggg caggaactcc tcgcgaacgc cggggcgaag atggagcaag atcggcgaga
     2701 cgtgcgtggc ccccgcgtcg atcgcggccg cgaccacctc gcggagctgg cgcggatcgt
     2761 cggtgatccc gggcaggatc ggggccatca ggacgccgca gggaatgccg gcctcgttca
     2821 gcgcccgaac agcgtcgatc cgcttgcgcg ggggcggggt gccgggctcg ctcgagcgcc
     2881 aggcggcctc gtcgagcgtc ccgatcgaga aggccgtgga gacgtccgtc acgcggccgg
     2941 cctcgaccag cagctcgagg tcccgcagga tcagcgtgcc cttggtcagg atcgagaacg
     3001 ggttccggta gtcggtgagc gcccggatga tccccggcat cagccggtag cgaccctcgg
     3061 cacgctggta cgggtcggtg ttggtgccca tcgcgattcc ctcgcccttc cagcgcttgg
     3121 ccgcgagctg gcgccggagc acctcggggg cgttgacctt gacgacgatc ttcttctcga
     3181 agtccgaacc ggcgttcatg ttcaggaatg tgtgggtcgg acgagcgaaa cagaatttgc
     3241 aggcatgact gcatcctcga tacgggttga tcgaccagtt gaacggcagg tagttgccgg
     3301 ggacgtggtt gatgatcgtc ttgcactcgg cctcgataaa ctccatcccc cggaactcgg
     3361 gcgtgtcgaa acgccgtctc gcgatctcga acaggggctc cggggccatc cgggcacgat
     3421 agaacatacg ttcgattcga gcaagcctcc ccatccggcc cgttgccgcc taggcttcgg
     3481 ccccgatggc gatctggctg gttcggcact gcgagaccga gtggtcgatc tcggggcggc
     3541 acaccgggag cacggacctc ccgctcacgc ccgagggacg tctgcaggcc acggcgatcg
     3601 gcaagctgct cgccggccgt ctgttcgacc acgttttctc gagcccgatg ctccgtgcgc
     3661 gggagaccgc ccggctcgcg gggttcgccg accgagtccg cgcgcacgag gggatgctcg
     3721 agtacgacta cggcgagttc gagggtctca ccaccgggca gatctgggag acccaccccg
     3781 ggtggcagct cttccgtgac ggttgccccg gcggcgagac gcccgagcag atggccaagc
     3841 gcgtcgacgt cctgatcgag gaacttcgcg agctcggggg caacagcctc ctgttcgggc
     3901 acggccactg cttccgtgcg ctggccgtcc gcttcctgga cctaccgatc cggtacgcga
     3961 cgaacctgct gctcgatgcg gggacggtct cgatcacctc ggacgcgcgt gacgggccgg
     4021 cgctcgtgct gtggaaccgc cgggtgcagc ctcgcgcgat gatcgtggcc gacatcgcca
     4081 tagcgatcgg ccgaacggtc ccctaggcaa ctcggctagg attcggcggt gggttgtctc
     4141 ttcgcgatcg tcgcgctcat cacgccccgc ttcatcctgt tcttcatgtg gctgttcacg
     4201 aactatctga acaccgcttt ctcgagcggc tggtgggggt tgctcgggtt cttcttcctc
     4261 ccgaccacca cgatcgccta cgcgatcgcc cagaacgagt tcacgaccgt gacgggcggg
     4321 gtcgaggcga cggggatcgt gctcatcgtg ctcggcatcg tgatcgactt gggactgctc
     4381 ggggggtccg gtcggggcgt cgcccgcagg ggctgagcca aagagcccta cgcgaaccgc
     4441 acccggggtt cgacgtacgc gtacgcgatg tcgacgcaca ggttggcgat cgcgatgaag
     4501 aacgccacga agatcgtgat ccccatcacc gacggaagtc gttgcgcaga tccggcaaac
     4561 atccccggcg ttcgcggaac cctgcgcggt cgttgcggtt tccctcccct cgaagaggtg
     4621 ggatagggtc cggccatggc cgacggggcg gctttcgagt tcgaggatgc gtcgatcgat
     4681 gagctccagg cctcgatggc ctcgggctcg gtcacctccc gcacactgac cgaggcctac
     4741 ctcgaacgga tcgagagagt cggcgcggcg ctccgctcgg tgctggagac gaaccccgat
     4801 gcgctcgaga tcgccgacac gctcgacgcc gagcgccgtt cgggccgcgt ccgggggccg
     4861 ctccacgggg ttcccgtgct cgtgaaggac aacatcgata ccgcggatcg catgaacacg
     4921 acggccgggt cgttcgcgct cggggaggcg acgccggcgc gcgacgcgtt cctggtcggg
     4981 cggctgcggg aggaaggcgc cgtgatcctc ggcaaggcca acctctccga gtgggcgaac
     5041 ttccgctcga cgcgttcgag cagcgggtgg agcgcccgcg gaggccagtg cgtgaacccg
     5101 tacgacccga accgcagccc gtgcggctcg agttcgggct cgggcgtcgc ggtatcggcg
     5161 aacctgtctg ccgtcgcagt cggcaccgag accgacggct cgatcgtctg cccctcgtcg
     5221 gtcaacggca tcgtcggcat caagcccacg gtcgggctgg tgagtcgctc cggcgttatc
     5281 ccggtgtcgc actcccagga cacgcccggc ccgatggcgc gaacggtcgc agacgccgcg
     5341 gcactgctga cggcgcttgc ggtcgccgac ccggccgacg ccgcgacggc cggggcgcgt
     5401 gtcgaacccg actacgccgc gttcgtcgac ccggacgggc ttcgggatgc gaggatcggg
     5461 gcggccagga acctcgcggg cttcaacgac cgcgtcgacc gactgtttga cgaggcgctc
     5521 gatgcgatgc gcgacctcgg cgccgagatc gtcgacccgg ccgaaatccc ccacaccgac
     5581 gatctcgagg accccgagct cgaggtgttc ctgtacgagt tcaaggccga cctcgaggca
     5641 tacctcggtt cgtacggccc gacacagcca catcggaccc tcgccgacct gatcgcttac
     5701 aacgaggagc acgttgacac cgagatgccg ttcttcggac aagagctctt cgagcaggcc
     5761 gcggagaagg gacccctgac cgagcccgcg tacctcgagg cgctggctgc ctgccggcgt
     5821 ctctcacggg ccgagggcat cgatgcggtg atggacgaac accgactgga cgcgctcgtc
     5881 gcgccctcga acgcgcccgc gtggcgcatc gactacgcca acggcgatca ctacgtgggc
     5941 gggaactcga cgcccgcggc ggtcgccgga tacccgagcg tcacggtccc gatggggttc
     6001 gcgttcgacc tgccgatcgg gatctccttc atcggacgtg cgtggagcga ggggacgttg
     6061 atccggctgg cctcggcgtt cgagcacgcg acgggtcacc gacgcccgcc cggtctcggg
     6121 cgaatttcct cgcaagatcg acgcgatcgt gcgcgaacga caggtgcgcg accggacgcc
     6181 aaggccggat aatcgagccc aggaccgccg cgaggggagg cctgcgatgg aaggctccgc
     6241 acccaccacg tcctcggacc gggggctcgc gagggaggcg atcggcctcc gcgaggtctt
     6301 ctttcaatcc gtcacgcaca tggcgccggc ggccgccgtc gcgttctcga tcatcgtggg
     6361 ggcgaacttc gcctcgggtg ccctgccgct gtcggtcatc ttcgcgctgg tcggctgcct
     6421 gctggtggcg atctcgatcg gccagctcgc gaagcacctg ccttcggccg gcggcttcta
     6481 cacgtacacg gccaggggga tccatccctc gatcggcttc ctggtggcgt gggggtacgc
     6541 gttcgtcgag ccgctggtc
//
LOCUS       DATBRK010000310         7866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_198900, whole genome shotgun sequence.
ACCESSION   DATBRK010000310 DATBRK010000000
VERSION     DATBRK010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198900"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VIH70_09290"
     CDS             complement(<1..671)
                     /locus_tag="VIH70_09290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8116594.1"
                     /translation="MNSFEVAVIGAGVHGASVAFHLATRGVRTAVIERTSPAGGPTGR
                     SSAVVRAYYTNAFLAGVARDSIAMFRDFEHHTGRDAGFRETGLLVLHPPEDEATVREV
                     VPRLTEQGIPTDLLEPQQVVAEWPAFDLDGIAIAAFERDAGYADPVLTTQGLLGRARE
                     LGAEIRLGHGVTAIKRAGSAWSVSTSGASVQAERVLISAGPWTAPLAATVGADLPLTV
                     ERHIVA"
     gene            complement(683..1012)
                     /locus_tag="VIH70_09295"
     CDS             complement(683..1012)
                     /locus_tag="VIH70_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116595.1"
                     /translation="MSSREERMALNEAASRETNEEIEQVHHNDPPLRRMTIACECALK
                     SCEEAIEITMAEYQDVRSDARQFAIVPEHFIGDIERIVFENDRFAVVAKRAGTPADVE
                     TETDPRS"
     gene            1130..1639
                     /locus_tag="VIH70_09300"
     CDS             1130..1639
                     /locus_tag="VIH70_09300"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEY8116596.1"
                     /translation="MAKDSDVPVDLYIAAYGDADAAQEDFDALKELVEDGLIFMDVAV
                     LVSRDAEGKLTVKENAHEVAKGGMVGALGGFVLGVIFPAGVIAATVVGGAAGAGIGGL
                     TSHHREREIQKELEDVLPPDSSGIVAIFDEVWVEQVEKALAKAEKIDKEQIDKESVEK
                     AKEAASKAS"
     gene            1667..1885
                     /locus_tag="VIH70_09305"
     CDS             1667..1885
                     /locus_tag="VIH70_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116597.1"
                     /translation="MLGIIGAGAGIPPEMSKATSWTMAQSFVLLLGYVLAFAIAVGLV
                     AWVDSKLSSRRLFREIYRELGIGRDPSR"
     gene            1936..2148
                     /locus_tag="VIH70_09310"
     CDS             1936..2148
                     /locus_tag="VIH70_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116598.1"
                     /translation="MDLPDALEQLKNATHSLKLFLSWAESQTEDQLREMSNDNWMALF
                     SAGHDHEERAIELDRELTRGKEARPS"
     gene            2294..2818
                     /locus_tag="VIH70_09315"
     CDS             2294..2818
                     /locus_tag="VIH70_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116599.1"
                     /translation="MKWVAAATLAGTGIWGGVTWAVILEGTEWRWIPVAIAVEAVVLA
                     GAIVTPKLRPKLLLGAGIAVVMTAAAVAYLLPRRWCFGHSWTGGPPWDCSDFPLWGRI
                     LVVGVAIILALILASFARDKPDRGHWAVGFGEALRARETRSTAGSPEREGALATEDAS
                     APSASHRRCACITN"
     gene            complement(3028..3600)
                     /locus_tag="VIH70_09320"
     CDS             complement(3028..3600)
                     /locus_tag="VIH70_09320"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY8116600.1"
                     /translation="MPKRCEWVPLDQPLYLAYHDEEWGVPVHEDTRLFEMLTLEGAQA
                     GLSWSTILNKREGYRRAFSGFDPVKVARFTPAKVERLLQDPGIVRNRMKVESTVNNAD
                     RVREVQREHGSLDTYLWSFVGGRPLVGRFKRLGDIPAETAESKAMSKGLKAKGFRFVG
                     PTVCYAFMQACGLAQDHVTSCFRYADLVGS"
     gene            3629..4849
                     /locus_tag="VIH70_09325"
     CDS             3629..4849
                     /locus_tag="VIH70_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY8116601.1"
                     /translation="MGAPWWTRPGLEARDGRLLVAGRDAEAVAREHGTPVFAYDLRRI
                     EEQARSLMEVFERAGVPFRLRLALKAQRDPEVLAFVRGLGAVGIDACSPREVQHALEH
                     GWSVEEISFTGTNLSAADVDALIETGVHVNLDLLSQLDRWGRRAPGTTVGVRVNPRVG
                     ASSNPELGDLYTGGDRPSKFGILVEQLDDALAIAREHDLAFDTVHLHVGRGVLTDALP
                     AFELAIERAASMTRRLADAGHPIEEVNVGGGLGVPHEAAQQPLDVEAFAGLLARHLRP
                     LGVRVACEPGDYLVKESGLLLAEVVTLDVRDGVTFAGLDAGFNVAPEHFIYGEPVPIV
                     LCRAADAPAEHTVTVAGNINEGDDLWGEDVPLPLLREGDVVALLRVGSYDRSMHIEHC
                     LRPPAGTVAFADRI"
     gene            complement(4862..5302)
                     /locus_tag="VIH70_09330"
     CDS             complement(4862..5302)
                     /locus_tag="VIH70_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116602.1"
                     /translation="MEDERARAQRAAPMLSYEDVGAAIEWLERAFGFRELEGERYTDE
                     EGRVTHAELVLDGALVMLGWPGPEYRGPARHAEECDVARRWLETPYVVDGVWIRVEDV
                     DAHFDRARAEGATILGELVDEPYGRLYRAADVEGHRWMFMRQAS"
     gene            complement(5312..6805)
                     /locus_tag="VIH70_09335"
     CDS             complement(5312..6805)
                     /locus_tag="VIH70_09335"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY8116603.1"
                     /translation="MERIGHYIGGKHVLGGSGRRGPVYDPATGEQTHEVDFADTAELD
                     QAVEAAKEALPAWRATSLSKRAEIMFRIRELLEARKTDVATVLTSQHGKVLSDAMGEV
                     QRGLENVEYATAIPHLLKGGFSEQVSTGVDVFEIRQPLGVVAGITPFNFPAMVPIWMC
                     ANAMACGNTFILKPSEKDPGAALVLAELFADAGLPDGVFNVVQGDKVAVDAILAHPDI
                     RAVSFVGSTSIARYIYTNGTANGKRVQALGGAKNHMIVLPDADVDMVADAAVSAGYGS
                     AGERCMAISVLTSVGSIADTVIDAIADRLPKIKVGPGLDPESEMGPLITRAHRDKVAS
                     YLDSGSAQGARIVADGRDHPLYKDSPGFFLAPSLMDDVTPEMDAYKDEIFGPVLSVTR
                     AETYDQAVDLVNRNSYGNGVALFTRDGGAARQFTFEVEAGMVGINVPIPVPVAYYSFG
                     GWKSSLFGDAHMYGPEGIRFYTRGKVVTQRWPDPAKSAVDLGFPRTR"
     gene            6889..>7866
                     /locus_tag="VIH70_09340"
     CDS             6889..>7866
                     /locus_tag="VIH70_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8116604.1"
                     /translation="MSDLTADEIVDLCLRHTLYDWQQQGSTRPIAVASARGCEFFTVD
                     GKRYLDFNSQLMGVNIGHADPRVADAIAEQARVLPYISPFMATEVRARLGVKLAELMP
                     GDLDKVFFTLGGAEANENAIRIAKATTGRQKILARYRSYHGATYATITLTGDPRRWAN
                     EPGMPGVVHVLDPYHGTQRGTDDAQTALAYLEETIMLEGPETIAAFILEPVTGTNGIL
                     IPPDGYLQGVRELCDRHGILLIADEVMSGFGRTGEWFAVNHWGVVPDIITMAKGLTSS
                     AVPLGAVGLSPKVYRYFDEHVFYGGLTYNSHPIGLAAALATIRVYEEDGL"
BASE COUNT         1232 a         2705 c         2695 g         1234 t
ORIGIN      
        1 gcgacgatgt ggcgctcgac cgtgaggggg aggtcggcgc ccaccgtagc cgcgagcggc
       61 gcggtccacg gacctgcgga gatcaggacg cgttcggcct gcaccgaggc accggacgtc
      121 gacacggacc aggccgagcc cgcgcgcttt atcgcggtga cgccgtgacc caggcggatc
      181 tcggccccga gctcgcgcgc gcgtcccagc aggccctgcg tcgtgagcac cggatcggcg
      241 taccccgcgt cgcgttcgaa cgccgcgatc gcgatcccgt cgaggtcgaa cgccggccac
      301 tccgccacca cctgctgcgg ttcgaggagg tcggtcggga tgccctgttc ggtcaggcgc
      361 ggcacgacct cgcgcaccgt cgcctcgtcc tcgggcggat gcagcaccag taggccggtc
      421 tcgcggaacc ccgcgtcccg cccggtgtgg tgctcgaagt cgcggaacat cgcgatcgag
      481 tcgcgagcca ccccggccag gaacgcgttc gtgtagtagg cgcgcacgac cgcgctcgaa
      541 cgcccggtcg ggcctcccgc gggcgacgtc cgctcgatca cggccgttcg gacaccgcgg
      601 gtcgcgaggt ggaacgcgac gctcgcgccg tgcacgccgg ccccgatcac cgcgacctcg
      661 aaggaattca tgccacggcg cgctagctcc gaggatcggt ctcggtttcc acgtcggccg
      721 gcgtgccggc gcgtttggcc acgacggcga agcggtcgtt ctcgaagacg atccgctcga
      781 tgtcccctat gaagtgctcg gggacgatcg cgaactgccg cgcgtcgctc cgcacgtcct
      841 ggtactccgc catcgtgatc tcgatggcct cctcgcacga cttgagggcg cactcgcagg
      901 cgatcgtcat ccgccgtaac ggcggatcgt tgtggtggac ctgctcgatc tcctcgttgg
      961 tctccctgga ggcggcctcg ttcagggcca tccgctcctc gcgcgagctc atgcccggga
     1021 ttatcccccg acgaagccgg gaccgggcga tctcggcgag acctcgggca cgcgagcacg
     1081 cgcgtagact cgacagccgg aaacaccctc ggatcgaagg agaccatcca tggcgaagga
     1141 ttcggatgtc ccggtcgacc tgtacatcgc cgcgtacggc gatgccgacg ccgcacagga
     1201 ggacttcgac gcgctcaagg agctggtcga ggacggcctg atcttcatgg acgtggccgt
     1261 gctcgtcagc cgcgacgccg agggcaagct cacggtgaag gagaacgctc acgaggtcgc
     1321 gaagggcggc atggtaggcg cgctcggcgg gttcgtcctc ggggtgatct tcccggccgg
     1381 ggtcatcgcg gcgaccgtgg tgggcggcgc ggccggtgcg gggatcggcg gactcacgtc
     1441 ccaccaccgc gaacgcgaga tccagaagga gctcgaggac gtcctgccgc cggactcctc
     1501 cggcatcgtc gccatcttcg acgaggtctg ggttgagcag gtcgagaagg cgctcgccaa
     1561 ggccgagaag atcgacaagg agcagatcga caaggagagc gtcgagaagg ccaaggaggc
     1621 cgcgagcaag gcgagctagg cgatcgaccg aaggagcgtc cgtcgcgtgc tgggaatcat
     1681 cggggccggc gccggcatcc cacccgagat gtcgaaggcg acctcgtgga ccatggcgca
     1741 aagcttcgtc ctgttgctcg ggtacgtgct ggcgttcgcg atcgccgtcg ggctcgtcgc
     1801 gtgggtggac agcaagttga gctcacgaag gctgttccgc gagatctacc gcgagctcgg
     1861 gatcggcaga gatccttctc ggtagcgggg tcaagcgtta ggctggggcg tcgccgcatc
     1921 cgaggggagg caaagatgga cctgccagat gcgctggagc agctgaagaa tgcgacccac
     1981 tctctcaagc tgttcctgtc ttgggccgag agtcagaccg aagatcagct gcgcgagatg
     2041 agcaacgaca actggatggc actcttcagc gccggccacg accatgagga gcgggcgatc
     2101 gagctcgatc gggaattgac ccggggcaaa gaggctcggc cgagctagcc ctcgagcaac
     2161 ggatgcattc gccgatccgc tcggcccgct cttcaacgcg gcgaatccca gtctgcttgt
     2221 gttccggcat cttgccagcg actttcgggg cgcgcaccat cgcgttccct tccgatgaca
     2281 ggagttccgg aagatgaagt gggttgcggc cgcgacactc gccggaaccg gcatctgggg
     2341 cggcgtcacc tgggccgtca tcctggaggg gaccgagtgg cgatggatcc cagtggcgat
     2401 agccgtcgaa gcggtcgtct tggccggcgc gattgtcact cccaaactcc ggccgaagct
     2461 gctgctcggc gcgggaatag ccgtggtcat gacagccgcc gcggtcgcgt acctactgcc
     2521 gcgcaggtgg tgcttcggcc atagctggac cggggggcct ccatgggatt gctcggattt
     2581 cccgctgtgg ggccgcatcc tcgtggtggg tgttgcaatc attctggccc tcatcctggc
     2641 gtccttcgcc cgggacaagc ctgacagagg acactgggcc gtcgggtttg gcgaagccct
     2701 gagagcgcgc gagacccgtt ccacagccgg aagtccggag cgtgaaggcg ccttggccac
     2761 agaagatgcg tcggcgccct ccgccagtca tcgccgctgc gcgtgcatca ccaactagag
     2821 atgcttcctt cggcgaaatg ctttgaccac tgagaaggtt gccccgagga ccattgccca
     2881 cagcgcaagg aacaggcccc attgccaccc gagagtgacg gctccgataa gtccatagat
     2941 tagggctccg gcgagagaga tcgacattac atatcgtgtc gagacgctca ccctgaccga
     3001 gacccaacga gagtcatttt tcgacggcta actgcccaca aggtcggcgt atcggaagca
     3061 cgacgtgacg tggtcctgcg cgagaccgca cgcctgcatg aacgcgtagc agacggtcgg
     3121 accgacgaat cggaacccct tcgccttcag gcccttgctc atggctttgg actccgcggt
     3181 ctcggcgggg atgtcgccga gtctcttgaa ccgacccacg agcggtcggc cgccgacgaa
     3241 cgaccagagg tatgtgtcga gcgacccgtg ctcgcgctgg acctcgcgca cgcggtctgc
     3301 gttgttcacg gtggactcga ccttcatccg gttccggacg atgcccgggt cctgcagcag
     3361 ccgttcgacc ttcgcgggcg tgaaccgcgc gaccttcacg gggtcgaacc cggagaacgc
     3421 gcgccggtag ccctcgcgct tgttgaggat cgtcgaccac gagagccccg cctgggcgcc
     3481 ctcgagcgtc agcatctcga acagccgcgt gtcctcgtgc accggcacgc cccattcctc
     3541 gtcgtggtac gccaggtaga gcggctggtc gagcgggacc cactcgcagc gcttcggcat
     3601 cggccgaacg ctagcatcgg cacccgacat gggcgcaccc tggtggacca gacccgggct
     3661 cgaggcccgc gacggccggc tcctcgtggc cggccgcgac gccgaggcgg tcgcccgtga
     3721 gcacgggacg cccgtgttcg cgtacgacct gcgtcggatc gaggagcagg cgcggagcct
     3781 gatggaggtg ttcgagcgtg ccggcgtccc gttccgactc cggctcgcgc tgaaggccca
     3841 gagggacccg gaggtgctcg cgttcgtgcg cggcctgggc gccgtgggga tcgacgcgtg
     3901 ttcgccccgc gaggttcagc acgcgctcga gcacggctgg tcggtggagg agatcagctt
     3961 cacggggacc aacctgtctg ccgccgatgt cgacgcgctc atcgagaccg gcgtccacgt
     4021 gaacctcgac ctgctctcgc agctcgaccg gtggggccgc cgcgcgccgg gcaccacggt
     4081 cggcgttcgc gtcaaccccc gcgtcggagc ctcctcgaac cccgagctgg gcgacctcta
     4141 cacgggaggc gaccggccgt ccaagttcgg gatcctggtc gaacagctcg acgacgcact
     4201 cgcgatcgct cgcgaacacg acctcgcgtt cgacacggtc catctgcatg tcggtcgagg
     4261 ggtgctcacg gacgcgcttc ccgcgttcga gctcgcgatc gaacgcgccg cgtcgatgac
     4321 ccggcggctc gcggacgccg gccatccgat cgaagaggtc aacgtgggag ggggactcgg
     4381 cgttccccac gaggcggcgc agcagccact cgacgtcgag gcgtttgcgg gcctcctcgc
     4441 ccgccacctg cgtcccctag gcgtcagggt cgcctgcgag cccggcgact acctcgtcaa
     4501 ggagtcgggc cttctgctcg ccgaggtcgt cacgctcgac gtccgcgacg gggtgacgtt
     4561 cgccggtctg gatgcggggt tcaacgtcgc gcccgaacac ttcatctacg gcgagccggt
     4621 cccgatcgtc ctgtgccgcg ccgccgatgc tccggccgaa cacaccgtta cggtggccgg
     4681 caacatcaac gagggggacg acctgtgggg ggaggacgtc ccgctgcccc tactgcgcga
     4741 gggagacgtg gtggccctgc tccgggtcgg cagctacgac cgctcgatgc acatcgagca
     4801 ctgcctccgc ccgcccgccg gcacggtcgc gttcgcggat cggatctgac tctcgggtgt
     4861 ctcaggaggc ctggcgcatg aacatccagc ggtggccctc gacatccgcg gctcggtaga
     4921 gccttccgta cggctcgtcg accagctcgc cgaggatcgt ggcgccctcg gcccgcgcgc
     4981 gatcgaagtg cgcgtccacg tcctcgacgc ggatccacac gccgtcgacg acgtacggcg
     5041 tctcgagcca gcgacgcgcc acgtcgcatt cctcggcgtg tcgggccggg ccccggtact
     5101 ccggcccggg ccacccgagc atcaccaggg cgccgtcgag gaccagctcc gcgtgcgtga
     5161 cacggccctc ctcgtcggtg tagcgctccc cttcgagctc tcggaaaccg aaggcacgtt
     5221 cgagccactc gatcgcggca ccgacgtcct cgtacgagag catcggggcc gcccgctggg
     5281 cgcgggcccg ttcgtcttcc atgtggctca tctagcgcgt gcgcgggaac ccgaggtcga
     5341 ccgcgctctt ggccgggtcc ggccagcgct gggtgacgac cttgccgcgc gtgtagaacc
     5401 ggatcccctc gggcccgtac atgtgcgcgt ccccgaacag cgagctcttc cacccgccga
     5461 acgagtagta cgcgaccggc accgggatcg gcacgttgat gcccaccatc cccgcctcga
     5521 cctcgaacgt gaactggcgc gccgccccgc cgtctcgcgt gaacagcgcc acgccgttgc
     5581 cgtacgagtt gcggttcacc aggtcgacgg cctggtcgta cgtctccgcc cgagtcaccg
     5641 agagcaccgg cccgaagatc tcgtccttgt acgcgtccat ctcgggcgtc acgtcgtcca
     5701 tcagcgacgg cgcgaggaag aacccggggc tgtccttgta gagcgggtgg tcacgaccgt
     5761 cggccacgat ccgcgcaccc tgtgccgaac cgctgtccag gtacgaggcc accttgtccc
     5821 ggtgcgcgcg ggtgatgagc gggcccatct cggactcggg atcgaggccc ggcccaacct
     5881 tgatcttggg caggcggtcg gcgatcgcgt cgatcaccgt gtcggcgatc gagcccacgc
     5941 tcgtcaggac cgagatcgcc atgcagcgct cgccggccga gccgtacccg gccgagaccg
     6001 ccgcgtcggc caccatgtcc acgtcggcgt ccggcaggac gatcatgtgg ttcttcgcgc
     6061 cgccgagcgc ctggacgcgc ttcccgttcg cggtgccgtt cgtgtagatg tagcgcgcga
     6121 tcgacgtcga gccgacgaag ctcaccgcac ggatgtcggg gtgcgcgagg atcgcgtcca
     6181 ccgccacctt gtcgccctgg acgacgttga acacgccgtc ggggagcccg gcgtcggcga
     6241 agagctccgc gagcaccaac gccgccccgg ggtccttctc ggagggcttg aggatgaacg
     6301 tgttgccgca cgccatcgcg ttcgcgcaca tccagatcgg gaccatcgcg gggaagttga
     6361 acggcgtgat gccggccacg acgccgagcg gctgacggat ctcgaacacg tcgacgccgg
     6421 tggacacctg ctcggagaag ccgcccttca gcagatgcgg gatcgcggtc gcgtactcca
     6481 cgttctcgag accccgctgg acctcgccca tcgcgtccga gagcaccttg ccgtgttgcg
     6541 acgtgaggac ggtcgcgacg tcggtcttgc gggcctcgag cagctcgcgg atcctgaaca
     6601 tgatctccgc gcgtttggac agcgaggtgg cgcgccaggc cgggagcgcc tccttggcgg
     6661 cctcgaccgc ctggtcgagc tcggcggtgt cggcgaagtc cacttcatgc gtctgctcac
     6721 cggtggcggg gtcgtacacg gggcctcgac gtccagatcc gccgaggacg tgcttgccgc
     6781 cgatgtagtg gccgatgcgc tccatggctt ccctccgggt gcgtgggcgt tgggaggatg
     6841 gtactcgccg gggctagact gccgctctcc agactcgagg gggatccgat gagcgacctg
     6901 accgccgacg agatcgtcga cctgtgtctg aggcacaccc tctacgactg gcagcagcag
     6961 ggatcgacgc gccccatcgc ggtcgcgtcc gcccgcggct gcgagttctt caccgtcgac
     7021 ggcaagcggt acctcgactt caacagccag ctgatggggg tgaacatcgg gcacgccgat
     7081 ccgagggtgg ccgacgcgat cgccgagcag gcgcgcgtgc tcccgtacat cagcccgttc
     7141 atggcgaccg aggtccgggc caggctcggc gtcaagctcg ccgagctcat gccgggcgac
     7201 ctcgacaagg tcttcttcac actgggcggc gccgaggcca acgagaacgc gatccggatc
     7261 gccaaggcga cgaccggccg gcagaagatc ctcgcgcggt accggtccta ccacggcgcg
     7321 acgtacgcga cgatcacgct gaccggcgac ccgcggcggt gggcgaacga gcccgggatg
     7381 ccgggcgtcg tccacgtgct cgacccgtac cacggcacgc agcgggggac cgacgacgcg
     7441 cagacggcgc ttgcgtatct cgaggagacg atcatgctcg agggccccga gacgatcgcc
     7501 gcgttcatcc tggaacccgt caccgggacg aacgggatcc tgatcccgcc cgatggctac
     7561 ctgcagggcg tccgcgagct ctgcgaccgg cacgggatcc tgctgatcgc cgacgaggtg
     7621 atgtcggggt tcggccggac cggcgagtgg ttcgcggtca atcactgggg cgtggtgccg
     7681 gacatcatca cgatggccaa gggcctgacc tcctcggcgg tgccgctggg cgcggtcggc
     7741 ctgtcgccga aggtctaccg ctacttcgac gagcacgtgt tctacggggg cctcacctac
     7801 aactcgcacc cgatcggcct ggcggcggcg ctcgcgacga tccgcgtgta cgaggaggac
     7861 gggctg
//
LOCUS       DATBRK010000311         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1351504, whole genome shotgun sequence.
ACCESSION   DATBRK010000311 DATBRK010000000
VERSION     DATBRK010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351504"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1261
                     /gene="rpoD"
                     /locus_tag="VIH70_09345"
     CDS             83..1261
                     /gene="rpoD"
                     /locus_tag="VIH70_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY8116605.1"
                     /translation="MAIAKEAQIDEVRDLVAKGKERGFLTNEEVIEALAPVDVSAEQM
                     DNILQHLQDENIEVVEMVDELDAEEFAREARSARDEELALKAPTNDPVRMYLKEIGKV
                     PLLTAEQEVILAKAIEEGEGATAEIDKATDGGRKLGSTRLRELQRTERQGQLAKKKLI
                     EANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQA
                     ITRAIADQARTIRIPVHMVETINKLIRIQRQLLQDLGREPTPEEIGREMEFSPEKVRE
                     ILKVSQEPVSLETPIGEEEDSHLGDFIEDSDAVVPVDAASFILLQEQLDSVLHTLSER
                     EKKVIQLRFGLTDGHPRTLEEVGREFGVTRERIRQIESKTLSKLRHPSRSQKLRDYLE
                     "
BASE COUNT          278 a          535 c          519 g          214 t
ORIGIN      
        1 gggggtctcc ccgagcgcac cgcgtaggtg gggttgcggc gctcccgcgt gggtccgcac
       61 ggaacgagag gtacctgagc agatggcgat cgccaaggag gcgcagatcg acgaggtccg
      121 tgacctcgtc gccaagggca aagagcgtgg cttcctcacc aacgaggagg tcatcgaggc
      181 gctcgccccg gtcgacgtct cggccgagca gatggacaac atcctgcagc acctgcagga
      241 cgagaacatc gaggtcgtcg agatggtcga cgagctcgat gccgaggagt tcgcgcgcga
      301 ggcacggtcc gcccgcgacg aggagctggc cctcaaggcc cccaccaacg accccgtccg
      361 catgtacctc aaggagatcg gcaaggtgcc gctcctgacc gcggagcagg aggtcatcct
      421 cgccaaggcg atcgaggagg gggagggcgc caccgccgag atcgacaagg cgaccgacgg
      481 cggccgcaag ctcggctcga cccggctgcg ggagctgcag cgcaccgagc gccagggcca
      541 gctcgccaag aagaagctga tcgaggccaa cctgcgcctg gtggtgtcga tcgccaagcg
      601 ctacgtcggc cgcggcatgc tgttcctgga cctgatccag gagggcaacc tcggcctcat
      661 ccgggcggtc gagaagttcg actacaccaa ggggtacaag ttctcgacct acgccacctg
      721 gtggatccgc caggcgatca cccgggcgat cgccgaccag gcccgcacca tccgcatccc
      781 ggtgcacatg gtcgagacga tcaacaagct gatccgcatc cagcgccagc tgctgcagga
      841 cctcggccgc gagccgaccc ccgaggagat cggccgcgag atggagttct cgcccgagaa
      901 ggtccgcgag atcctcaagg tcagccagga gccggtcagc ctggagacgc cgatcggtga
      961 ggaggaggac tcccacctcg gcgacttcat cgaggactcc gacgcggtgg taccggtcga
     1021 cgccgccagc ttcatcctgc tgcaggagca gctcgactcg gtgctgcaca ccctctccga
     1081 gcgggagaag aaggtgatcc agctccgctt cggcctcacc gacggccacc cgcgcacgct
     1141 ggaggaggtc ggccgcgagt tcggcgtcac ccgcgagcgg atccgccaga tcgagtccaa
     1201 gaccctctcc aagctgcggc acccctcccg cagccagaag ctccgggact acctggagta
     1261 gcgcgctggg tcctggccac cgctcgcctc atcgccccct cgggggagtg gagcgcccgc
     1321 gccgggtggt gcgcttcgcg cgattgaccc atgcggcagg cgcgcgttct tggcggacct
     1381 tcggcccgcg ccgcctacgg cggcgacctc aagcctgacc tgattgccac gcaccgtggt
     1441 accgcatggc cagcgtgatg tccctcctac ggctcgaacc ctggctagag acgggctttg
     1501 gtgaggtagt tgtcctgctg tggaatcggc acgccctgcc gcacaa
//
LOCUS       DATBRK010000312         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_54413, whole genome shotgun sequence.
ACCESSION   DATBRK010000312 DATBRK010000000
VERSION     DATBRK010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54413"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..4095
                     /locus_tag="VIH70_09350"
     CDS             145..4095
                     /locus_tag="VIH70_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8116606.1"
                     /translation="MDERPGGPSGRSAALRDLLWILLAVLLLMVLGSATRLFEPFYGW
                     VGERLRGQVGEAVGALVLLAAGLSLFALLQGRSNRQESSARHQAENRYRALVEKMPAV
                     IYTRDPRRSAGTMAAPYVSPQIEQILGFTAQEWTDAPALWIRRVHDEDRDRVLAASEV
                     ADRTGEPLTIEYRSIRKDGTVIWVHEEAVTIERDRRGRPSLSQGVMYDVTERKRAEQD
                     LAETEARYRTLVERVPAVTYTWDAATRGGDMAPSYVSPQVEEMLGYPADEFRDPRLWS
                     ELVHGDDVQRVRAEWAICKRGKGAFRSEYRMHTRDGRELWVRDEAVPVRRDDDGHSLY
                     QGVMFDITERKVAEQLLQKAEANYRTLVEQLPVVVYQDAIDDVSTALYISPQYERLFG
                     FHPDVRLRDPQFWVDHLHPEDRDRVLELSRWTNETGEPFSAEYRFLGRGGRVVWVRDE
                     AELLRDADGQPMMWQGVLLDVTARKHAEDALTRRDAVLQAIGFAAERFLRSDDWAAAL
                     PDVLERLGTASSAGRVYVFENIALEDGRPGMRLYRQWLGPGIVGFGDGAMTSQAYDDG
                     FERWRSVLSERRELHGLTRDFPEHERRHLEGEGVRSRIVVPVSVGEEWWGFIGFDDCA
                     TERVWPAAEVEALKAAADTLGAAIGRTRSEQQRLEEQAAREGLELRTSAVLASALEAI
                     VTMDEDGRIVDFNPAAERMFGFQREDVAGEPLADVLVPPELRSRHLTGLARFLETGRT
                     TILGTHLEVEALRADGERLPIELTVTQVPIPGTTLFTGFIRDLTERRHAELQLREAEQ
                     RYRTLVENLPAATYIDLVDDVFTTLYVSPQIESIWGYTPQEWTADPNVWIDNLHPDDR
                     DRVLEAFARHHEQGEPFEVEYRLRTKDGRWTWVGDHATVARDEEGRIAFSQGAMFDVT
                     ERRQAENQLREAEERYRAIVEHVPAAIYVDVPDGTMQSRYVSPQIEQIMGCTPEEFIA
                     EPELWLDLMVDEQQREEMRTTYVAAITERRPSWTGEYLIRHRDGREVWVHDETTLVTD
                     QRGEPLLLQGVMYDVTERKLAERALLESEAAEREAAERLRAVDEMKNTFLAAVSHELR
                     SPLTSILGLSLTLEQQPLPVPEQEELLGRLAVNARKLDRLLKDLLDIDRLSRGIVTPK
                     VRASDLASIVGRTVESLDVLDQRSILVQAENVIVPVDAPKIERIVENLVMNAVRHTEP
                     DVGIWVRVWAHDGGAVIAVEDDGPGVPVELRHEIFEPFRQGPTSSPHSPGTGIGLSLV
                     TMFAELHGGRAWIEDREGGGASFRVFLPGDTADRLTGNGHEQGAPAGIPSEVAG"
     gene            4106..>5013
                     /gene="lipA"
                     /locus_tag="VIH70_09355"
     CDS             4106..>5013
                     /gene="lipA"
                     /locus_tag="VIH70_09355"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY8116607.1"
                     /translation="MPVREHNPLQRRLYLPIISTDGPPAATERKPPWLKVRLQRGPNY
                     RELESIMRARSLHTVCEEAMCPNISECWEEREATFLILGDRCTRRCGFCDVMTAKPDE
                     VDEDEPAKIADAVREMGLRFVVVTGVARDDLPDGGARIWASTIRAIRDAVPGCGVEVL
                     PTDFKGGERDIATVIQAEPDVFAHNLETVRRLHGRIRPAFGYDRTLEVLRTARRLREG
                     QVTKSNLILGMGERADEIPQALRDLADAGARIVTIGQYLRPTEHHLPVDRWVHPDEFA
                     KWKELGESFGIAHVEAGPLVRSSYHAG"
BASE COUNT          825 a         1715 c         1780 g          693 t
ORIGIN      
        1 cgacatcggc gagcagatgc tcgagctcgc caggttctaa ccgagcacgc gcacaccggg
       61 gccggcacgg gccgcaacga ccttcggtct ccccctcccc ggctcaggga agtccccgag
      121 aggtccgata cctcaggcac agccatggac gaacgacccg gtggcccgag cggcaggtcc
      181 gcggcgctgc gggatctgct gtggatcctg ctcgccgtgc tcctcctcat ggtgctcggc
      241 tcagcgaccc ggctgttcga gcccttctac ggctgggtcg gcgaacggct ccgcggtcag
      301 gtcggcgagg ccgtcggcgc gctcgtgctc ctcgcggcgg ggctctcgct gttcgcgctc
      361 ctgcagggac ggtcgaaccg acaggaaagc tcggctcggc accaggcgga gaaccgctac
      421 cgggcgctcg tcgagaagat gccggccgtc atctataccc gggacccccg ccggtccgcc
      481 ggcacgatgg cagcaccgta cgtgagccca cagatcgagc agatcctcgg gttcaccgcg
      541 caggaatgga ccgacgcccc cgccctgtgg atccggaggg tgcacgacga ggatcgggac
      601 cgcgtcctgg cggcgtcgga ggtggccgac cgcaccggcg aaccgctcac catcgagtac
      661 cgcagcatcc gcaaggacgg cacggtgatc tgggtgcacg aggaggcggt cacgatcgaa
      721 cgagaccgcc gaggccgtcc ctcgctgtcc caaggcgtga tgtacgacgt caccgagcgc
      781 aagcgcgccg agcaggatct cgccgagacg gaggctcgct atcgcacgct cgtggagcgc
      841 gtgcccgcgg tgacgtacac gtgggacgcc gccacccgcg gcggcgacat ggccccgtcg
      901 tacgtcagcc cccaggtcga ggagatgctc ggctatccgg cggacgagtt ccgagatcct
      961 cggctgtggt ccgagctcgt gcacggggac gacgtgcaac gcgtccgggc cgaatgggcg
     1021 atctgcaaac ggggcaaggg agccttccgt tcggagtatc ggatgcatac gcgcgacgga
     1081 cgtgagctct gggtccgcga cgaagcggtc cccgtccgac gcgacgacga cggccactcg
     1141 ctgtaccagg gcgtgatgtt cgacatcacc gagcgaaagg tcgcggagca gctccttcag
     1201 aaggccgagg ccaactatcg gaccctggtc gaacagctcc cggtcgtcgt ctaccaggac
     1261 gcgatcgacg acgtcagcac cgcgctgtac atcagcccgc agtacgaacg actgttcggc
     1321 ttccatccgg acgtccgact gcgggatccc cagttctggg tcgaccatct gcaccccgaa
     1381 gaccgcgacc gcgtcctcga gctctcgcgc tggacgaacg agaccggcga gcccttctcg
     1441 gccgaatacc gcttcctcgg acggggcggg cgcgtcgtgt gggtacgcga cgaggcggag
     1501 ctgctccgcg acgcggacgg gcaaccgatg atgtggcagg gggtcctgct cgacgtcacg
     1561 gcccgcaagc acgccgagga tgcgctcacc cgtcgcgacg cggtcctgca ggcgatcggg
     1621 ttcgcggccg agcggttcct caggagcgac gactgggcgg ccgcccttcc cgacgtcctg
     1681 gagcgacttg ggacggcgtc gagcgccggc cgcgtctacg tgttcgagaa catcgcgctg
     1741 gaggacgggc gccccggcat gcggctgtac cggcagtggc tcggtcctgg catcgtgggg
     1801 ttcggcgacg gcgcgatgac gagccaggcg tacgacgacg gtttcgaacg gtggcgctcg
     1861 gttctgtcgg agcgccggga gctccacggg ctgacccggg acttccccga gcacgagcgg
     1921 cgccacctcg agggcgaagg ggtccggtcg cggatcgtgg tcccggtctc ggtgggcgag
     1981 gagtggtggg ggttcatcgg cttcgacgac tgcgcgaccg aacgcgtgtg gccggccgcc
     2041 gaggtcgagg cgctcaaggc ggcggccgac accctgggcg ccgcgatcgg tcgcacccgc
     2101 tccgagcagc aacgcctcga ggaacaggcc gcccgggagg ggctggagct ccgtaccagc
     2161 gccgtactgg cctcggcgct cgaggcgatc gtcacgatgg atgaggacgg caggatcgtg
     2221 gacttcaacc ccgcggcgga gcggatgttc ggcttccagc gcgaggacgt ggcgggggag
     2281 ccactcgccg acgtcctggt cccgcccgag ctgcgatcgc gccacctcac cggcttggcc
     2341 cggttcctcg agaccggccg gacgacgatc ctgggcacgc acctcgaggt ggaggcgctc
     2401 cgtgccgacg gggaacgcct cccgatcgag ctgacggtca cccaggtccc gatacccggc
     2461 acgacgctgt tcaccgggtt catccgcgac ctcaccgagc ggcggcacgc cgagctccag
     2521 ctccgagagg ccgagcagcg gtaccgcacg ctcgtcgaga accttcccgc ggccacgtac
     2581 atcgacctgg tcgacgatgt gttcacgacg ctgtacgtga gccctcagat cgagtcgatc
     2641 tggggctaca cgccgcagga gtggacggca gaccccaacg tctggatcga caacctgcac
     2701 cccgacgatc gcgatcgcgt cctcgaggcc ttcgcccgcc accacgagca gggcgaaccg
     2761 ttcgaggtcg agtaccgcct ccgcacgaag gacggtcgat ggacgtgggt gggagaccac
     2821 gcgacggtcg ctcgcgacga ggagggacgg atcgccttct cgcagggcgc gatgttcgac
     2881 gtgaccgaac gccgccaggc cgagaaccag ctccgcgagg ccgaggagcg ctatcgcgcg
     2941 atcgtcgagc acgtcccggc cgcgatctac gtggacgtgc cggacggcac catgcagagc
     3001 cggtacgtga gcccgcagat cgaacagatc atggggtgca cacccgagga gttcatcgcc
     3061 gagcccgagc tctggctcga cctgatggtg gacgagcagc agcgggagga gatgcgcaca
     3121 acctacgtcg ccgcgatcac ggagcggcgg ccctcgtgga ccggcgaata cctgatccgc
     3181 catcgggacg ggcgcgaggt gtgggtgcac gacgagacca cgttggtgac cgaccaacgc
     3241 ggggagccgc tgttgctgca gggcgtgatg tacgacgtga ccgaacgcaa gctggccgaa
     3301 cgcgccctcc tcgagagcga ggccgcggaa cgcgaggccg cggaacgcct gcgggcggtc
     3361 gacgagatga agaacacgtt cctcgcggcc gtctcgcacg agctccggag cccgctgacg
     3421 tcaatcctcg ggctctcgct cacgctcgag cagcagcctc tgcccgtccc ggagcaggag
     3481 gagctgctcg gccggctggc ggtgaacgca cgcaagctcg accggctgct caaggatctc
     3541 ctcgacatcg accgcctctc ccgagggatc gtgacgccga aggtgcgggc ctccgacctc
     3601 gcatccatcg tggggcggac ggtcgagagc ctggacgttc tcgatcagcg gtcgatcctg
     3661 gtccaggccg agaacgtcat cgtgcccgtc gacgcgccaa agatcgagcg gatcgtcgag
     3721 aacctcgtga tgaacgccgt ccgccacacc gagcccgacg tggggatctg ggtgcgggtc
     3781 tgggcgcatg acggcggggc cgtcatcgcg gtcgaagacg acggaccggg ggtgcccgtc
     3841 gagctccggc acgagatctt cgaaccgttc cggcaggggc cgacctcgtc cccacactcg
     3901 ccggggaccg gcatcggcct ctcgctggtg acgatgttcg ccgagctgca cggcggacgc
     3961 gcctggatcg aggatcgcga gggaggcggg gcgtcgttcc gcgtcttcct gccgggcgac
     4021 accgccgacc gcctgacggg gaacggtcac gagcagggcg cacccgcggg catcccaagc
     4081 gaggtcgcag gctagactgg ctgcgatgcc cgttcgggaa cacaacccgc tccagcgacg
     4141 cctctacctg ccgatcatct cgacggacgg gccgcccgcc gccaccgagc gcaagccgcc
     4201 gtggctcaag gttcggctgc agcgcggccc caactaccga gagctcgaat ccatcatgcg
     4261 cgcgcgctcg ctccacaccg tgtgcgagga agcgatgtgc ccgaacatct ccgagtgctg
     4321 ggaggagcgg gaggcgacgt ttctgatcct cggggacagg tgtacgcggc gttgcggctt
     4381 ctgcgacgtg atgacggcca aacccgatga ggtggacgag gacgaaccgg ccaagatcgc
     4441 cgacgccgtc cgagagatgg ggctccgctt cgtcgtggtg accggcgtgg cccgcgacga
     4501 cctccccgac gggggcgcgc ggatctgggc atcgaccatc cgggcgatcc gcgatgccgt
     4561 tcccgggtgc ggagtggagg tgctgccgac cgacttcaag ggcggcgaac gcgacatcgc
     4621 gacggtgatc caggccgagc cggacgtgtt cgcgcacaac ctcgagaccg tgcgccggct
     4681 gcacggccgt atccggccgg cgttcggcta cgaccgcacg ctcgaggtat tgcgcaccgc
     4741 gcggcggctg cgcgagggcc aggtcacgaa gtcgaacctg atcctcggca tgggcgagcg
     4801 cgccgacgag atcccgcagg cgctgcgcga cctggccgac gccggcgcgc ggatcgtgac
     4861 gatcggccag tacctccggc cgaccgagca ccatctgccc gtcgaccggt gggtccaccc
     4921 ggacgagttc gcgaagtgga aggaactcgg ggaatcgttc gggatcgcac acgtcgaggc
     4981 cggtcccctg gtgaggagtt cctaccacgc cgg
//
LOCUS       DATBRK010000313         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1064196, whole genome shotgun sequence.
ACCESSION   DATBRK010000313 DATBRK010000000
VERSION     DATBRK010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064196"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VIH70_09360"
     CDS             complement(<1..1541)
                     /locus_tag="VIH70_09360"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8116608.1"
                     /translation="MGDDGTAADRLLERYWEGLLEVEPLLGTAIGDERFDDRLPDPGP
                     NGRAERERLHRGALEELGLLEVDEFDEDRRVSLDILEAIARRDLAVLEHRTDRLSAVS
                     HLWGPAGLAGELASLQRADTPERIERYAARIAATPAFYDAELEIMREGIADGVTAPRI
                     VAERALAQTERLIEAGAEDSPVLAPVPAEDADGRARLAAAVADHLLPALERYRQGIRD
                     YLPNATETIGLRDLPGGEAMYAAQILSWTTLELDAREVHELGTADLEKIQEERARAAA
                     ILGHPDAATAEAAIAGDAAFRFGSPEELKATAEEQVRRSWDLAPTWFGRVPSANCEVR
                     LVEEFREADMPFAFYNQPTEDGSRPGVYYVNGFDLARKPTHQLAPTTYHEANPGHHFQ
                     VALEQEMDGRPALRRFGGFLAGSAFTEGWGLYSERLADEMGLYLDEAERLGMLDLQGM
                     RAARLVVDTGIHALGWTRERAIETLEGAGLPTVEATIETDRYITMPGQALSYKIGQFE
                     IERQRA"
     gene            1753..>2178
                     /locus_tag="VIH70_09365"
     CDS             1753..>2178
                     /locus_tag="VIH70_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116609.1"
                     /translation="MLVRACALLYLTGLAACMGSPEVVGSAGGQPASSDRSLEPARGP
                     ARGSSGTGDPGSEGSGSRGPASPPATGPYEGLGTWIDIYDTDVWEHPVPAVRSMAAHG
                     IRTIYLQSSNYHRHRPFVHPEGVRAIVDAAHRRRLEVVAW"
BASE COUNT          272 a          788 c          779 g          339 t
ORIGIN      
        1 gcgcgctgcc gttcgatctc gaactgcccg atcttgtacg agagcgcctg tccgggcatc
       61 gtgatgtaac gatcggtctc gatcgtggcc tcgacggtgg gcagcccggc gccctcgagc
      121 gtctcgatcg cgcgctccct cgtccacccg agcgcgtgga ttcccgtgtc cacgaccagg
      181 cgggcggccc gcatcccctg caggtcgagc atccccagcc gttccgcctc gtccaggtac
      241 aggcccatct cgtcggccaa ccgttcgctg tacaggcccc atccctcggt gaacgcgctc
      301 ccggccagga acccgccgaa ccgacgcagc gccggccggc catccatctc ctgctcgagc
      361 gcgacctgga agtggtggcc cgggttggcc tcgtggtacg tggtcggggc gagctgatgt
      421 gtcggcttgc gggccaggtc gaaaccgttc acgtagtaca cgccgggccg ggacccatcc
      481 tcggtcggct ggttgtagaa cgcgaacggc atgtcggcct cccggaactc ctcgacgagg
      541 cggacctcgc agttggcgct cgggaccctg ccgaaccacg tcggcgccag gtcccagctc
      601 cgccggacct gctcttccgc tgtcgccttg agctcctccg gcgacccgaa gcggaacgcc
      661 gcgtcccccg cgatcgcggc ctcggcggtg gcggcgtcgg ggtggccgag gatggcggcc
      721 gctcgcgcgc gttcttcctg gatcttctcc aggtcggcgg tgccgagctc gtggacttcg
      781 cgagcgtcga gctcgagcgt cgtccatgac aggatctggg ccgcgtacat cgcctcgccg
      841 cccgggaggt cccgcaggcc gatcgtttcg gtcgcgttcg gcaggtagtc gcggatgcct
      901 tggcggtacc gctcgagcgc cgggaggagg tggtccgcga ccgcagcggc cagccgcgca
      961 cgcccgtcgg catcctcggc ggggacgggt gcgaggacgg gtgagtcctc ggcgcccgcc
     1021 tcgatcagcc gctcggtctg cgcgagcgcg cgttcggcga cgatccgcgg ggccgtgacc
     1081 ccatcggcga tcccctcgcg catgatctcg agctcggcgt cgtagaaggc cggggtcgcc
     1141 gcgatccggg cggcgtagcg ttcgatgcgt tcgggcgtgt cggcgcgctg gagcgacgcg
     1201 agctcgcccg ccaggcccgc gggaccccag aggtggctca ccgcgctcag ccggtcggtc
     1261 cggtgctcga gaacggcgag atcgcggcgc gcgatcgcct cgaggatgtc gaggctgacc
     1321 cgtcggtcct cgtcgaactc gtcgacctcg agcagcccga gctcctcgag cgcgccgcgg
     1381 tgaaggcgct cgcgctcggc gcgaccgttc ggtccggggt cggggagccg gtcgtcgaag
     1441 cgctcgtccc cgatcgccgt cccgagtagc ggctcgacct cgagcagccc ctcccaatag
     1501 cgttcgagca gccggtccgc cgcggtgccg tcgtccccca tcgttccttc gcccccaagg
     1561 tcctccgaag agcgctcatc ctagcgggcg gcgcccgaac atccggcgat ccgatccgag
     1621 cccgacccat ttttcgagcc cgcccccgat cgaacagaac cttggggcga tccggacggt
     1681 ctctgttctc cggaccggct ccagaggggt tctccgggac cggcacagca ggggggatgg
     1741 tgcatgagac cgatgctcgt gcgcgcgtgc gcgctgctct atctgacggg gctcgcggcg
     1801 tgcatgggtt cgcccgaggt ggtcgggtcg gcgggggggc agcccgcatc gtccgatcgg
     1861 tcgctcgagc cggcgagggg gcccgcccgg ggctcctccg gcaccggtga ccccgggtcg
     1921 gagggatccg gttcgcgggg accggcgtca cccccggcca cggggccgta cgagggcctc
     1981 ggaacctgga tcgacatcta cgacacggac gtgtgggagc acccggtgcc cgcggttcgg
     2041 tccatggccg cgcacggcat ccggacgatc tacctccaga gctcgaacta ccaccgacac
     2101 cggccgttcg tgcacccgga gggcgtgcgg gcgatcgtgg acgccgcgca ccgacggcgt
     2161 ctcgaggtgg tggcgtgg
//
LOCUS       DATBRK010000314         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1213004, whole genome shotgun sequence.
ACCESSION   DATBRK010000314 DATBRK010000000
VERSION     DATBRK010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213004"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VIH70_09370"
     CDS             <1..199
                     /locus_tag="VIH70_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8116610.1"
                     /translation="IRQRSGEDGPWIIAMTANVMQGDREAYLAAGMDDYVSKPIRPGE
                     LAEALAKVPTRRAISNQPSEG"
     gene            330..668
                     /locus_tag="VIH70_09375"
     CDS             330..668
                     /locus_tag="VIH70_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8116611.1"
                     /translation="MTEAVDHRVLAALSEATGDDPAFLAELIDTYLADAPDLLMAMRD
                     ALAAREAAGLRRAAHTLKSTSATLGAHRLADLCRHLEDAEGSAETLVAQAEDEYLRVR
                     VDLQAARPPS"
     gene            987..1694
                     /locus_tag="VIH70_09380"
     CDS             987..1694
                     /locus_tag="VIH70_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8116612.1"
                     /translation="MTEGPGFVLVVDDDAVNRLLLSRALERDGHQVRAVANGLEALQA
                     LRDGLFDCVLLDVLMPEMDGYQVLGHIRSDPRLRRTPVIMISALEDTESVVRCIEMGA
                     DDYLPKPFDPVLLRARINAGLARKRLDDLEQEYLEQVGHVVAAAAAVESGTFESGQLD
                     GVARRGDALGQLARVFQRMATEVQARERRLTQEVHKLRIEIDEVRAARQVAEITETEY
                     FQDLQRKVSELRLDPNA"
     gene            1732..2586
                     /locus_tag="VIH70_09385"
     CDS             1732..2586
                     /locus_tag="VIH70_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEY8116613.1"
                     /translation="MSRVISIHSFRGGTGKSNTAANVATLLAAQGQRIGVVDTDLQSP
                     GIHALFGLDQEALRYSLNEYLWGRCDIKQAAYDLTTNLDAGLRGRVFLVPSSIKVNDI
                     ARVMREGYDVGLLNEGLRRLVKELSLDVLMLDTHPGLNEETLLSIAMSNTLAIVMRPD
                     QQDYEGTRVTVSVARKLQVPDLVLVVNKTPPVFDPDEVRQRVEKAYECEVTAVLPHSD
                     ELMTPGSAGIFALRYPEYPVTGLYRQIAARLTAYRLPWPPTRWIGSPIETSSSLVFPP
                     GGPTPSCS"
     gene            2511..>3166
                     /locus_tag="VIH70_09390"
     CDS             2511..>3166
                     /locus_tag="VIH70_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116614.1"
                     /translation="MDRFPDRDELLAGLPTRRADTLLFLIESRTAHLLAGSPRAMEQF
                     VTEADRDERELAFFAAFSQGLDPPLVPTIQDLERHSRQWAPLVAESPQVRAAVAHRLG
                     KKYRFTYQDSAGIRRALGLDDPEVRRAYRRLYRRPLEEIFAARVAPADRLRWAWAAVG
                     ARLERLSPFWTVYALTLTETVGATILALPIALATIGPLPGVAMLVALGLVNIVTIACM
                     A"
BASE COUNT          527 a         1073 c         1103 g          463 t
ORIGIN      
        1 gatccgccag cggtctggcg aggacgggcc gtggatcatc gcgatgaccg ccaacgtcat
       61 gcagggcgac cgcgaggcct atctcgcagc gggcatggac gactacgtca gcaaaccgat
      121 ccggccagga gagctcgccg aggcgttggc caaggtaccg accaggcgtg ccatctcgaa
      181 ccagccctcg gagggttagc aactggctgt agatctcgcg atgcgtgccg tgatcaagag
      241 cggcggcccg ccaaaaggag caggagccgg cagcggagta ccgcgaagcg gtcgtcagcc
      301 gcgcatcagc cggacgaggg gaagggacag tgaccgaggc ggtcgaccac cgggtgctcg
      361 cagcgttgtc ggaggcgacc ggcgacgatc cggccttcct ggccgagctg atcgacacgt
      421 atctggctga cgcgccagac ctgctgatgg cgatgcgcga cgcactggcc gcgcgcgagg
      481 ccgcggggct gcgccgggct gcgcacacgc tgaagtcgac cagtgcgacg ttgggcgcgc
      541 accggctcgc cgacctgtgc cgccacctcg aggatgccga gggctccgcc gagaccctcg
      601 tggcgcaggc cgaggacgag tacctccgag tccgcgtcga tctccaggcc gctcgcccgc
      661 cgtcctgaca gattccccac tggctgcagg gctcatctcg ggcgaagtcg cacatcaggc
      721 tgaatccgcg catctgccct accgagcctg tggactcttc tgtccaatac acgcagaatg
      781 cctgggggaa gctccggacc tctgtcgcgg acgggcagaa gggacctcca agcgctcgag
      841 cgtgagccct acgacgcgct aacggacgtg cagatgccga ggatggacgc gctgtgccgc
      901 cgccctcacg tcgccccaca gtccccagct ccggcaggcg aatccggtga gggcccgaag
      961 gcgcggcggg agcagcaggg ccgaccatga ccgagggtcc cgggttcgtc ctcgtggtcg
     1021 acgacgacgc ggtgaaccgc ctgctcctgt cccgagccct cgagcgagac ggccatcagg
     1081 tgcgggcggt ggcgaacggg ttggaggcgc tccaggcgct ccgcgacggg ctgttcgact
     1141 gcgtgctgct ggacgtgctg atgcccgaga tggacggcta ccaggtgctg gggcacatca
     1201 ggagtgaccc caggctgcgg cgcacgccgg tgatcatgat ctcggcgctc gaggacaccg
     1261 agagcgtcgt caggtgcatc gagatgggcg cggacgacta cctgcccaag ccgttcgacc
     1321 cggtgctgct tcgtgccagg atcaacgcgg ggcttgccag gaaacgcctc gacgacctcg
     1381 agcaggaata cctcgagcag gtgggccacg tcgtcgcggc ggcggcggcc gtcgagtcgg
     1441 gaaccttcga gagcgggcag ctggacggcg tcgcccgccg aggcgacgcc ctcgggcaac
     1501 tggcacgcgt cttccaacgg atggcgaccg aagtccaggc gcgcgagcgg cgcctgaccc
     1561 aggaggtgca caagctgcgc atcgagatcg acgaggtgag ggccgcccgg caggtggccg
     1621 agatcaccga gaccgagtac ttccaggacc tccagcggaa ggtctccgag ctccgcttgg
     1681 acccgaacgc ataggtaggg cgggcacagc agcaaccgaa aaggagcggg gatgtcgagg
     1741 gtcatctcga tccattcgtt tcgcgggggc acgggcaagt cgaacacggc ggccaacgtg
     1801 gcgacgctgc tggccgcaca aggccaacgc atcggggtcg tcgacaccga cctgcagtct
     1861 cccgggatcc acgcgctgtt cgggctcgac caggaagcgc tgcgctactc gctcaacgag
     1921 tacctctggg ggcgctgcga catcaagcag gccgcttacg acctgacgac gaacctcgat
     1981 gccggactcc gcgggcgtgt gtttctcgtc ccttccagca tcaaggtcaa cgacatcgct
     2041 cgcgtgatgc gcgagggcta cgacgtgggc ctgctgaacg aagggctgcg ccggctcgtc
     2101 aaggagcttt cgctcgacgt gctgatgcta gacacccatc cgggcttgaa cgaggagacg
     2161 ctgctgtcga tcgccatgtc gaacacgctg gcgatcgtga tgcgcccgga ccagcaggac
     2221 tacgagggca ccagggtgac cgtgtcggtg gcccgcaagc tgcaggtgcc tgacctggtg
     2281 ctcgtggtca acaagacgcc gccggtcttc gaccctgacg aggtcagaca gcgggtggag
     2341 aaggcctacg agtgcgaggt gacggcggtg ctgccgcact cggacgagct catgaccccg
     2401 ggcagcgcgg gcatcttcgc gctgcgctat ccggagtacc ccgtcacggg cctgtaccgg
     2461 cagatcgccg cccgtctcac ggcctacagg ctgccgtggc ccccgacgag atggatcggt
     2521 tccccgatcg agacgagctc ctcgctggtc ttcccaccag gcgggccgac accctcctgt
     2581 tcctgatcga gagccgaacc gcgcacctgc tggccgggtc tccccgggcg atggagcagt
     2641 tcgtcaccga agcggaccgc gacgagcggg aactcgcctt cttcgcggcc ttctcgcagg
     2701 gcctggatcc gccgctggtc ccgaccatcc aggatctcga acgccactcc cggcagtggg
     2761 cgcccctggt cgcggagagc ccgcaggtcc gcgctgccgt cgcccatcgc ctggggaaga
     2821 agtaccggtt cacgtatcaa gattcggccg gcatccggcg ggcgctgggg ctcgacgacc
     2881 cggaggtcag acgggcatac cgccgtctct accggcgacc actcgaggag atcttcgccg
     2941 cccgggtggc tcccgccgac cgcctgcgct gggcgtgggc cgccgtgggc gcgcggctcg
     3001 agcgcttgtc cccgttctgg accgtctacg ccttgacgct gaccgagacg gtgggcgcga
     3061 ccatcctcgc cctgcccatc gcccttgcga ccatcggccc gctgcccggc gtggcgatgc
     3121 tggtggcgct cggcctggtc aacatcgtga cgatcgcgtg catggc
//
LOCUS       DATBRK010000315         5311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_774447, whole genome shotgun sequence.
ACCESSION   DATBRK010000315 DATBRK010000000
VERSION     DATBRK010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774447"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /gene="ruvA"
                     /locus_tag="VIH70_09395"
     CDS             complement(<1..435)
                     /gene="ruvA"
                     /locus_tag="VIH70_09395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY8116615.1"
                     /translation="MISFLEGDVAERSGARVVISVGGVGYDVQVPASTLAKLPPVGRP
                     TRIYTRMVVRDDAITLFGFANADERELFDLLVTVNGIGPRVALSFLSVLSPGAFRRAV
                     SSGDVAALTVVPGVGKKVAQRVVLDLKDKLGAEDVVIIDGPLA"
     gene            complement(432..971)
                     /locus_tag="VIH70_09400"
     CDS             complement(432..971)
                     /locus_tag="VIH70_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY8116616.1"
                     /translation="MFDRGTAIPQTEQVFDACVLGVDPGVSALGLAAVGRRDRKPVLL
                     FADTVRTPSDLPDASRLRLLHDAVAEVIATHQPGSVAIERIAWNKNQVSAMIVARATG
                     VILLAVAEAGLEVEEYGSLEVKMAVTGQGNADKAQVRVALERFHGLEGLPDLPDAVDA
                     AAIALCHLTQARLRKVAAP"
     gene            complement(1367..1624)
                     /locus_tag="VIH70_09405"
     CDS             complement(1367..1624)
                     /locus_tag="VIH70_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116617.1"
                     /translation="MDEVQRAIVRPVRDAEEPIDIARREAGEELGRDLDVREFAAAIR
                     ELLESDMVQLWRTDARTGERTEMYALPSLIELDTTPSTLMS"
     gene            complement(1676..2089)
                     /locus_tag="VIH70_09410"
     CDS             complement(1676..2089)
                     /locus_tag="VIH70_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116618.1"
                     /translation="MNAHVSSILLGVRDMDRSKKFYMEGLGWKVKNDYGISVFFEPNG
                     GSLVGFYGREGLAVDNGASPEGSGFSGLVLTYVVRSEERVDEILAEAEKAGATILKPA
                     ATLEWGGYGGSFADPDGYIWSIGYSAQGKDQPYAE"
     gene            complement(2198..2737)
                     /locus_tag="VIH70_09415"
     CDS             complement(2198..2737)
                     /locus_tag="VIH70_09415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEY8116619.1"
                     /translation="MRERMLAGELYLADDPVLAEEQARAARLLDRFNQTSDGAELHGL
                     LFELLGKLGEGSEIKPPLRCDYGYRIRVGARTFVNYGLVALDVASIAIGDDVQIGPNV
                     QLLTPTHSIDPDTRRAKWEAAEPIAIASNVWLGGGVIVLPGVTIGENTVVGAGSIVTK
                     DLPPNIVAAGNPARIVRSL"
     gene            complement(2770..3525)
                     /locus_tag="VIH70_09420"
     CDS             complement(2770..3525)
                     /locus_tag="VIH70_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8116620.1"
                     /translation="MSGHSKWATIKRRKGAADAKRSNQFAKLLRAVEVAAREGGSGDV
                     KANMTLASAVDKARSFSVPVENIERAIKRGSGDLEGAARYEEVSYEGYGPGGVAVFVE
                     TLTDNRNRTTQDVRHAFTRSGGNLGENGSTAWMFARKGSIQVEKDAAPDEEKLLEIAL
                     EAGAEDLGDSESTWEITSDPASFTAVRDAIEAAGIPMMSAELTMLPQSTIPVEGGQAK
                     QVLALLEALEELDDVQNVYANFDIPESVMAELG"
     gene            complement(3527..4084)
                     /gene="pdxT"
                     /locus_tag="VIH70_09425"
     CDS             complement(3527..4084)
                     /gene="pdxT"
                     /locus_tag="VIH70_09425"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEY8116621.1"
                     /translation="MKAGVLALQGDFREHALVFEELGASPVEVRTPDDLARVDVLAIP
                     GGESTTISKLARSSGLVEPIVERAKAGMPILGTCAGMIVMARRVDGLEPLLSLVDVTV
                     RRNAYGRQVDSFEADLGVEGVPHPVRGVFIRAPIVDEVGEGVRVLAEHEGRPVVLEQG
                     NLVVASFHPELVGETGLHGYVLGKV"
     gene            complement(4092..4979)
                     /gene="pdxS"
                     /locus_tag="VIH70_09430"
     CDS             complement(4092..4979)
                     /gene="pdxS"
                     /locus_tag="VIH70_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEY8116622.1"
                     /translation="MSVERGTIRVKRGLAEMLKGGVIMDVTTADQAKIAEDAGACAVM
                     ALERVPADIRAEGGVARMADATKVEEIMNAVSVPVMAKARIGHFVEAQILQALGVDYV
                     DESEVLTPADEEHHIDKWAFTVPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVVE
                     AVRHQRTMLREIGWLRSLRPEQLVAAAKDLGAPVDLVRMVSETGKLPVVNFAAGGLAT
                     PADAALMMQLGVDGVFVGSGIFKSEDPAPRARAIVEATTHFQDPEALAKVSRGLGEPM
                     PGLDIKELGEEGLLQTRGW"
     gene            complement(5047..>5311)
                     /locus_tag="VIH70_09435"
     CDS             complement(5047..>5311)
                     /locus_tag="VIH70_09435"
                     /inference="COORDINATES: protein motif:HMM:NF037564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HEY8116623.1"
                     /translation="RNVIVMFIHTKHGNHKDAAGNPTPDGVVTGGGRAFLLRNGVLVK
                     GTWSRATLADPTTFTDSTGAPFKLLPGKTWIELAPPTASVSVA"
BASE COUNT          804 a         1872 c         1725 g          910 t
ORIGIN      
        1 ggccagcgga ccgtcgatga tcaccacgtc ctccgcgccg agcttgtcct tcaggtccag
       61 gaccacccgc tgcgcgacct tcttcccgac gcccgggacg acggtgagcg cggcgacatc
      121 gcccgaggag accgcgcgcc ggaacgcgcc gggactgagg acgctcagga aggagagggc
      181 gaccctgggg ccgatgccgt tcacggtgac gaggaggtcg aagagctcgc gctcgtcggc
      241 gttggcgaag ccgaacagcg tgatcgcgtc gtcgcgcacg accatccgcg tgtagatgcg
      301 tgtcggtcgt ccgacgggcg gaagcttcgc gagcgtcgac gccggtacct gcacgtcgta
      361 gccgacgccg ccgaccgaga tcaccacgcg cgcccccgag cgttcggcga cgtcgccctc
      421 caggaaggag atcacggcgc ggcgaccttg cgcagccgcg cctgcgtgag gtgacacagc
      481 gcgatcgcgg cggcgtcgac cgcgtccgga aggtccggca acccctcgag accgtggaag
      541 cgctcgaggg ctacgcgcac ctgcgccttg tccgcgttcc cctgcccggt gaccgccatc
      601 ttgacctcga gcgatccgta ctcttcgacc tcgagcccgg cctcggcgac ggcgagcagg
      661 atcacgccgg tggctctcgc cacgatcatc gcgctcacct ggttcttgtt ccaggcgatc
      721 cgctcgatcg cgaccgaccc gggctggtgt gtcgcgatga cttccgcgac cgcgtcgtgg
      781 agcagccgaa gtctcgaggc gtcgggaagg tccgacggcg tccgcacggt atcggcgaac
      841 aggagcaccg gtttccggtc gcgcctgccg acggcagcga gaccgagcgc ggatacaccc
      901 gggtcgaccc cgagcacgca ggcatcgaac acctgttcag tctgtgggat ggccgtgccg
      961 cggtcaaaca cctgggcgct agaccatagg ccctcaaagg aatcggagac agtgtctcag
     1021 cgtcctcagg aatcggggtc cgtgccccaa cacgtgatcc cgcttatcga tcgttcgaag
     1081 ctcttgctcg gagagtcgtc aatcctccga ttggcgtcgc tcacactcgc cgttcactcc
     1141 gatctgttcc ttggctgtgt cgacccctcg attcgaagtc tgtcgccgac ctcaattctt
     1201 cgctccgtgc agaactcgac ctcaggattc tcatcgtaca tccgacggtt ggctcgtttc
     1261 tcggacgaag cgtctgcctc cacaaggaat gagtgatgct taaagtcgac gtgtatccgc
     1321 caccgcgggg gcccgtcctt ctcgacgttc ggaccaagac ccagcgtcag ctcatcaggg
     1381 tcgagggtgt ggtgtccagt tcgatgaggg acggaagggc atacatctcc gttcgttcgc
     1441 cggtgcgggc gtcagtacgc cagagctgaa ccatgtcact ctcaaggagt tcgcgtatgg
     1501 cggcggcaaa ttctcggacg tcgagatcac gcccgagttc ctcccccgct tctcgccgcg
     1561 caatgtcgat aggttcttcg gcgtccctga cgggtctaac gattgctctt tgaacttcat
     1621 ccatcgcgcc tcagagctta ggaaatcggt aacgtcatgc gttcctcggc ggcggctact
     1681 ccgcgtacgg ctgatccttt ccctgggcgc tgtagccgat gctccagatg tagccgtccg
     1741 ggtcggcgaa ggagccgccg tacccgcccc actccagggt ggcggccggc ttgaggatcg
     1801 ttgcgccggc cttctcggct tccgccagga tctcgtcgac gcgctcctcg ctgcggacca
     1861 cgtaagtaag gaccagcccg ctgaagccgc tgccctccgg gctcgcgcca ttgtcgacgg
     1921 ccaggccttc gcggccgtag aagccgacga gcgagccgcc gttgggctcg aagaacaccg
     1981 agatgccgta gtcgttcttg accttccagc cgagaccctc catgtagaac ttcttcgacc
     2041 ggtccatgtc acggacgccg aggaggatcg agctcacgtg cgcgttcatg ccttgcctcc
     2101 tttcctgttg gggggacggt ccccctatga agccaccata ggtgcggccc gggggccgcc
     2161 gcttctcgat tcctgatcgg tatggggccc caatgtctca gagcgaccga acgatacggg
     2221 cggggttccc ggccgccacg atgttcgggg gcagatcctt cgtcacgatc gacccggcgc
     2281 cgaccacggt gttctccccg atcgtgacgc ccgggagcac gatgacccca ccgcccagcc
     2341 agacgttcga tgcgatcgcg atcggttcgg cagcctccca cttggcccgt ctcgtgtcgg
     2401 ggtcgatgga gtgcgtgggc gtcaagagct gcacgttggg accgatctgc acgtcgtcgc
     2461 cgatcgcgat cgatgcgacg tcgagcgcga cgagtccgta gttcacgaac gtccgcgcac
     2521 cgacccggat cctgtacccg tagtcgcatc gcagtggcgg cttgatctcg gacccttcgc
     2581 cgagcttccc gaggagctcg aacaagagac catgcagctc ggcgccgtcg cttgtttggt
     2641 tgaagcggtc gaggagcctc gccgcccgtg cctgttcctc ggcgaggacg gggtcgtcgg
     2701 ccaggtagag ctctccggcc agcatgcgct cgcgcatcgt ccgttcgccg gcggggcgct
     2761 cgggcgtcct cacccgagct cggccatcac cgattcgggg atgtcgaagt tcgcgtagac
     2821 gttctggacg tcgtcgagct cctcgagcgc ctcgagcagc gccagcacct gcttggcctg
     2881 gccgccctcg accgggatcg tgctttgggg cagcatcgtc agctcggccg acatcatcgg
     2941 gatcccggcc gcctcgatcg cgtcgcggac cgcggtgaac gaggccgggt cgctcgtgat
     3001 ctcccaggtg ctctcggagt cgcccaagtc ctcggcgccg gcctcgaggg cgatctcgag
     3061 caacttctcc tcgtccggcg ccgcgtcctt ctcgacctgg atcgagccct tgcgagcgaa
     3121 catccacgcc gtgctgccgt tctcccccag gttcccgccg ctgcgcgtga acgcgtgccg
     3181 gacgtcctgg gtggtgcggt tgcgattgtc ggtcagcgtt tcgacgaaca cggcgacgcc
     3241 gccggggccg tagccctcgt acgagacctc ctcgtaccgg gcggcgccct cgaggtctcc
     3301 gctcccgcgc ttgatcgcgc gctcgatgtt ctcgaccggc accgagaagc tgcgcgcctt
     3361 gtcgacggcc gaggcgagcg tcatgttcgc cttgacgtcc ccgcttccgc cctcacgggc
     3421 ggcaacctcg acggcgcgga ggagcttcgc gaactggttg ctccgcttgg cgtcggcggc
     3481 gcccttcctg cgcttgatcg tcgcccactt cgaatgaccc gacacgtcac acctttccca
     3541 gcacgtaccc gtgcagaccg gtctcgccca cgagctcggg atggaaggat gccacgacca
     3601 ggttcccctg ctccaggacg accggacgcc cctcgtgctc ggcgagcacc cgaaccccct
     3661 cgccgacctc gtcgacgatc ggggcgcgga tgaagacccc ccggacgggg tgcggcacgc
     3721 cctcgacgcc gaggtcggcc tcgaacgaat cgacctggcg accgtaggcg ttcctgcgca
     3781 ccgtgacgtc gaccagcgag agcagcggct cgagaccgtc gacccgccgc gccatcacga
     3841 tcatgcccgc gcacgtcccg aggatcggca tgccggcttt ggcgcgctcg acgatcggct
     3901 ccaccaggcc ggagctgcgc gcgagcttgg agatcgtcgt ggactccccg ccggggatcg
     3961 cgaggacgtc gacccgcgcc agatcgtcgg gggtacggac ctccaccggc gacgcgccga
     4021 gctcctcgaa cacgagggcg tgctcgcgga agtctccctg gagcgcgagg accccggcct
     4081 tcacgggcgg ttcaccaccc gcgggtctgg agcagaccct cctcgccgag ctccttgatg
     4141 tcgagcccgg gcatcggctc gccgaggccg cgcgagacct tcgcgagcgc ctcgggatcc
     4201 tggaagtgcg tggtggcctc gacgatggcg cgggcgcgcg gcgccgggtc ctcggacttg
     4261 aagatcccgg agccgacgaa cacgccgtcg accccgagct gcatcatgag cgcggcgtcg
     4321 gcaggtgtag cgagcccgcc ggccgcgaag ttcacgaccg ggagcttccc ggtctccgag
     4381 accatccgga cgaggtcgac cggcgcgccg agatccttgg cggccgcgac gagctgctcg
     4441 ggacgcaggc tccggagcca cccgatctcg cggagcatcg tccgctggtg gcggacggcc
     4501 tcgaccacgt tgcccgtgcc ggcctcgccc ttggtgcgga tcatcgccgc gccctcggcg
     4561 atgcgccgca gggcctcgcc caggttccgg gcgccgcaga cgaacgggac cgtgaaggcc
     4621 cacttgtcga tgtggtgctc ctcgtcggcc ggcgtgagca cctcggactc gtccacgtaa
     4681 tcgacgccga gcgcctggag tatctgtgcc tccacgaaat ggccgatgcg ggccttggcc
     4741 atcaccggca cggagaccgc gttcatgatc tcctcgacct tggtggcgtc ggccatgcgc
     4801 gcgaccccgc cctcggcgcg gatgtcggcc ggaacgcgct cgagcgccat cacggcgcac
     4861 gcgcccgcgt cctcggcgat cttggcctgg tcggccgtgg tgacgtccat gatgacgccg
     4921 cccttgagca tctcggcgag gcctcgcttg actcggatcg taccgcgctc gacgctcatg
     4981 atcgcctccc gaacacgact tgaagaattc agcatatacg cgcgctacga ggcaagcgca
     5041 agggatttac gcgacgctga ccgacgcggt cggcggcgca agctcgatcc acgtcttccc
     5101 gggcaagagc ttgaacgggg cgcccgttga gtcggtgaac gtggtcggat cggccagcgt
     5161 cgcccgcgac cacgttccct tgacgagcac accgttccgc aacaggaacg cgcgcccgcc
     5221 gccggtcacg acgccgtcgg gcgtcggatt tccggccgcg tccttgtggt taccgtgctt
     5281 ggtatggatg aacatcacga tcacattgcg a
//
LOCUS       DATBRK010000316         3997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2655207, whole genome shotgun sequence.
ACCESSION   DATBRK010000316 DATBRK010000000
VERSION     DATBRK010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655207"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VIH70_09440"
     CDS             complement(<1..444)
                     /locus_tag="VIH70_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009733917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8116624.1"
                     /translation="MNAAAIEVADVSKRFRIYRDKPTSLKQRVLSSRSRAEDFWALRD
                     VALDVGQGSTFGLIGHNGSGKTTLLKCVAGILRPTSGTIRQRGRLAALLELGAGFHPE
                     LTGRENVYLNASFLGLSRRQTDAAFDDIVAFAELEDFIDNEVKFYS"
     gene            complement(441..1346)
                     /locus_tag="VIH70_09445"
     CDS             complement(441..1346)
                     /locus_tag="VIH70_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8116625.1"
                     /translation="MATTQQTVERTSESPHLRVGERLRILAAHREILANLVRKELKVK
                     YAASVLGAVWSLLNPLVYLAVFSFVAKVLGAGIPNYPVYLLSGLLAWNLFSASMGSGA
                     RAVLDNANLVKKVAFAREILPLSAVGVGLVDFALQSAVLLLYIVVSGYGLHLPELALW
                     PLAFVTLLVLTTALSLWFSAWNVRYRDVGHLLNIALLVWFWATPIVYAEYQVQHLASE
                     NDVGGAGAIALYFLNPMVGIVAGFHRALYGTVTPPGAGSPVLFHESLAWVAGVVTITF
                     VVSLVGLRLAWGYFFNRSGDFAEEL"
     gene            complement(1701..3068)
                     /locus_tag="VIH70_09450"
     CDS             complement(1701..3068)
                     /locus_tag="VIH70_09450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8116626.1"
                     /translation="MNRVTTPIVGAVRRLYRWDHKMDPRVAWLSPMPPARSGIATYSR
                     AVLEGLERIGYTPAKHKIQPYWPIRHQHWATVPWHTLGAYHLGNNLEFHGDIYDLAIR
                     TPGLLVIHDLALDDFVMGMVATAQPFGHQALREGLLLAPRLHGFEEAERNEPLRVPYV
                     AHAARNARGIVVHSPFVERYLRAFGCKTPIFVAPHPVIESEPRVRKAESAARVIRGSL
                     ESMGMGTLIGVFGDQNAAKLIDVVLEAMVHLPQDVHLALVGRRIPGYDTEPLVRASGL
                     GARVSLHTDVSDEDFLAWMCAADVAVDLRYPHRGEVSGSLSRSMQCGRPTVVSATGTY
                     LDLPEDVVVRVPAGRLEPRELAEALRGLVDDSDRRRRIGDAARTRSAELAASEATARV
                     YAEAMDATMALLWDPARRALARWGGALVDLGITEEGLSEGYGMAYARALDEFRSSGSS
                     ASSLG"
     gene            complement(3071..>3997)
                     /locus_tag="VIH70_09455"
     CDS             complement(3071..>3997)
                     /locus_tag="VIH70_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8116627.1"
                     /translation="GKWYPATEIVHQMAVWRALDLSESNGLPIDMVIALKFPAYLVPH
                     ERKVVWLIHQHRTAYELWDHPEFADLGKQIEGPQVRDMVQQSDRIALGEAKRIFTNSR
                     NVQDRLWSSLRLSSEVLYHPSPIVEALLPMEPGPHGDAIVFPSRLEGLKRQALVVDAM
                     RYVKTGVRLVLVGNGPDEDGLREQIERHGLQSRVELAGRVSNERLHELYLGALGVYYG
                     PFDEDYGYVTLEGFAARRPVVTLTDAGGPLEFVTDGETGLVTPPEPQAIAHAFDRLFS
                     NRPLAQKMGETGNEKLRATVPGWPEVVARLTE"
BASE COUNT          638 a         1392 c         1401 g          566 t
ORIGIN      
        1 cgagtagaac ttcacctcgt tgtcgatgaa gtcctcgagc tcggcgaacg cgacgatgtc
       61 gtcgaacgcg gcgtcggttt gccgccgaga caaccccagg aacgaggcgt tcaggtagac
      121 gttctcgcgc cccgtcagct cggggtggaa cccggctccg agctcgagca gcgccgcgag
      181 ccgcccgcgc tggcggatcg tccccgaggt cggccgcagg atcccggcga cgcacttcag
      241 gagcgtcgtc ttgcccgagc cgttgtgccc gatcagcccg aacgtcgaac cctggcccac
      301 gtcgagggcg acgtcgcgga gcgcccagaa gtcctcggct cggctccgcg aggagaggac
      361 gcgctgcttg agcgacgtcg gcttgtcccg gtagatgcgg aagcgcttcg agacgtcggc
      421 gacctcgatc gcggcggcgt tcacagctcc tccgcgaagt cgcccgaacg gttgaagaag
      481 tagccccacg cgagccggag cccgacgagc gagacgacga acgtgatcgt cacgaccccg
      541 gcgacccacg ccaggctctc gtggaacagg accggactgc cggcacccgg gggtgtcacc
      601 gtcccgtaga gcgcccggtg gaacccggcg acgatcccga ccatcgggtt caggaagtag
      661 agggcgatcg cccccgcgcc gccgacatcg ttctcggagg cgagatgctg cacctggtat
      721 tcggcgtaca cgatcggcgt cgcccagaac cagacgagga gcgcgatgtt cagcaggtgg
      781 ccgacgtcgc ggtagcgcac gttccatgcc gagaaccaca gcgacagcgc cgtcgtcagg
      841 accaagagcg tcacgaaggc gagcggccac aacgcaagct cgggcaagtg gaggccatag
      901 ccggagacga cgatgtagag gagcaggacc gcggactgca acgcgaagtc gaccaggccg
      961 acaccgacgg ccgacagcgg caggatctcg cgcgcgaacg ccaccttctt cacgaggttc
     1021 gcgttatcga gcaccgcccg cgcgccgctg cccatcgagg ccgagaacag gttccaggcg
     1081 aggagtccgg acagcaggta aacggggtag ttcgggatcc cggcgcccag gaccttcgcc
     1141 acgaacgaga agaccgcgag gtagacgagc gggttcagca gcgaccacac ggccccgagc
     1201 accgaagccg cgtacttcac cttgagctcc ttgcggacca ggttcgccag gatctcccgg
     1261 tgggcggcga ggatgcggag gcgttcgccg acccgaaggt gcggcgactc ggaggtgcgc
     1321 tcgacggtct gctgcgttgt cgccatgggt gttgggcggg tcgtggccgg tcggctcgcg
     1381 gtcggcccgc gccgtgcgtg ccggcccgcg tggcgagtct agcaacggcc cccgcgccac
     1441 ccgtggagaa cgcacggaaa cgtcacggtc cgtgcacgtg gcacgtgggg gctcggacgt
     1501 gacggtgggc gcctgtgctc gagagcgggc gtcagggtgc tgactgacag ggacctgacg
     1561 ccgggtcgca ctcaacggga gttggcgtca gggttctgac agtcagtgat ctgacgccgg
     1621 cctcgaacca gaccaagggt ctcccgacgc gacagacgcg cgacgcgaca gacgcgcgac
     1681 gcgacggact cggcccgtcc tcagccgagc gaggacgcgc tcgagccgga cgatcggaac
     1741 tcgtcgagcg ctcgggcgta ggccatcccg tagccctccg agagcccctc ctccgtgatc
     1801 ccgaggtcta ccagcgcccc gccccaacgg gcgagcgcgc gccgggccgg gtcccacagg
     1861 agcgccatcg tcgcgtccat cgcctcggcg tagacgcggg cggtcgcctc ggacgccgcg
     1921 agctcggccg agcgcgttcg cgccgcgtcg ccgatccggc ggcgtcgatc ggaatcgtcg
     1981 acgagccctc tgagcgcctc ggccagctcg cgcggctcga gccgccccgc cggcacgcgg
     2041 acgacgacgt cctcggggag gtcgaggtag gtccccgtcg cgctcacgac cgtggggcgc
     2101 ccgcactgca tcgagcgcga gagcgacccg gacacctccc cgcggtgcgg gtaccggagg
     2161 tccaccgcca cgtccgcggc gcacatccac gcgaggaagt cctcgtccga gacgtcggtg
     2221 tgcagcgaca cgcgggcccc cagaccgctc gctcgcacca gcggctccgt gtcgtacccg
     2281 gggatccgtc ggccgacgag cgcgagatgg acgtcctgcg gcaggtgcac catcgcctcg
     2341 aggacgacat cgatcagctt ggcggcgttc tggtcgccga acacaccgat cagcgtcccc
     2401 atccccatcg actcgagcga ccctcggatc acgcgagcgg cgctctccgc cttgcggacg
     2461 cgcggctcgc tctcgatcac cgggtgcggg gcgacgaaga tcggggtctt gcagccgaac
     2521 gcgcgcaggt agcgttcgac gaacggcgag tggacgacga tgccccgcgc gttccgcgcc
     2581 gcgtgcgcca cgtatgggac gcggagcggc tcgttgcgtt cggcctcctc gaacccgtgg
     2641 aggcgcggcg cgagcagcaa cccctcacgc aacgcctggt gcccgaacgg ctgcgccgtc
     2701 gccaccatgc ccatcacgaa gtcgtcgagc gcgaggtcgt ggatcaccag gaggcccggc
     2761 gttcggatcg cgaggtcgta gatgtcgccg tggaattcca ggttgttgcc gaggtggtag
     2821 gcgcccaacg tgtgccacgg caccgtggcc cagtgctggt gcctgatcgg ccagtacggc
     2881 tggatcttgt gcttcgcggg cgtgtagccg atgcgttcga gaccctcgag caccgcccgc
     2941 gagtacgtcg cgatcccgct gcgagccggc ggcatcggcg agagccacgc gacgcgcggg
     3001 tccatcttgt ggtcccagcg gtagagccgt cgaacggcgc cgacgatcgg ggtggtcacg
     3061 cggttcacgg tcactccgtc agccgggcga cgacctcggg ccagcccggc accgtcgcgc
     3121 ggagcttctc gttgcccgtc tcgcccatct tctgggcgag cggacggttc gagaacaggc
     3181 ggtcgaacgc gtgggcgatc gcctgcggtt cggggggggt gaccaggccg gtctcgccgt
     3241 cggtcacgaa ctcgagcggc ccacccgcgt cggtgagcgt gacgacgggc cggcgcgctg
     3301 cgaacccctc gagcgtcacg tacccgtagt cctcgtcgaa cggcccgtag tacacgccga
     3361 gcgccccgag gtagagctcg tggaggcgtt cgttcgagac gcgaccggcg agctcgaccc
     3421 tgctttggag cccgtgcctc tcgatctgct cgcggaggcc gtcctcgtcg ggaccgttgc
     3481 cgacgagcac gagccgaacc ccggtcttca cgtaccgcat cgcatccacg acgagcgcct
     3541 gacgcttgag tccctcgagc cggctgggga acacgatcgc gtcgccgtgg ggtcccggct
     3601 ccatcggcag cagcgcctcg acgatcggcg acgggtggta caggacctcg ctcgacaacc
     3661 tgagcgagct ccacaggcgg tcctgcacgt tccgcgagtt cgtgaagatg cgcttggcct
     3721 ccccgagcgc gatccggtcg ctctgctgga ccatgtcgcg gacctgcggc ccctcgatct
     3781 gcttgccgag gtcggcgaac tcagggtgat cccacagctc gtacgccgtc cggtgctggt
     3841 ggatcagcca cacgaccttg cgttcgtgcg ggacgaggta ggcggggaac ttcagcgcga
     3901 tcaccatgtc gatcgggagc ccgttggatt cggacaggtc gagcgcgcgc cacaccgcca
     3961 tctggtgcac gatctcggtc gccggatacc acttgcc
//
LOCUS       DATBRK010000317         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1354147, whole genome shotgun sequence.
ACCESSION   DATBRK010000317 DATBRK010000000
VERSION     DATBRK010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354147"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VIH70_09460"
     CDS             complement(<1..570)
                     /locus_tag="VIH70_09460"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEY8116628.1"
                     /translation="MAVDDRDVRRAPEREPSSTGLGLMKGLAVTLKHALRPSITQQYP
                     QEKPDLPPRTRGVIALKEANCTVCYKCSRECPDWCIYIDAHKETHEPASGGKGRSMKV
                     LDRFAIDYALCMYCGICVEVCPFDALFWSPEFEYSEYDIVELTHEKEKLEEWTYSVLP
                     PPFIEDGAEVPVEEPVSAPSVAPDTAGPAA"
     gene            complement(579..1532)
                     /gene="nuoH"
                     /locus_tag="VIH70_09465"
     CDS             complement(579..1532)
                     /gene="nuoH"
                     /locus_tag="VIH70_09465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY8116629.1"
                     /translation="MVLVTKAAIVLIFFLVAPLGVGYAEHKVMAHMQGRLGPMYAGRF
                     HGWAQLLADGAKFVQKEDVIPAAADAWVFSLAPAVALVPYILLFVVIPFSDQIFILNL
                     DVGIFFVLALSSVSVIGVLMAGWASANKFSLIGALRAAAQLIAYELPLVLGAAAIVMQ
                     AETLSMVGIVEAQEKYWFVFSQPIGFLIFLTASLAELTRPPFDMPVADSEIIFGAYTE
                     YTGLKFAFFLLAEYGGIVALSAIASVLFLGGYLPLPALEFIPEPLWMLAKIGALSFLI
                     IWLRVTYPRLREDQLQRVSWLTLIPLALLQIVVVGVFKVAF"
     gene            complement(1583..2035)
                     /locus_tag="VIH70_09470"
     CDS             complement(1583..2035)
                     /locus_tag="VIH70_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY8116630.1"
                     /translation="MLSFEDQHGHSVVAVTPSRYRELVTFLRNEPEFACDYCDFISAV
                     DFGDEGMEVVTHVFSTTHVHSVRVKVRLPAEEAVLPTISDIYPTANWHERETTEMFGV
                     TFEGHPLPVTLLLPEPFEGHPLRKDFPLMSREAKPWPGAAEGEEEDDE"
     gene            complement(2073..2609)
                     /locus_tag="VIH70_09475"
     CDS             complement(2073..2609)
                     /locus_tag="VIH70_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009715926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY8116631.1"
                     /translation="MAQGGVEVGLITEKTPLAGIQPVKFLLNWGRRYSLWVFNFGLAC
                     CSIEFIAASTSKHDFIRFGVIPFAHGPRQADLIVIAGTPTDKMTPVIKRLYDQMPEPK
                     YVISMGSCANCGGPYWDSYSVTKGIDQVLPVDVYVPGCPPRPEALLQGIVRLQEKIAA
                     EDIREKWSGKHTTPASAS"
     gene            complement(2579..2968)
                     /locus_tag="VIH70_09480"
     CDS             complement(2579..2968)
                     /locus_tag="VIH70_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018490760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY8116632.1"
                     /translation="MLGKTFTSVETYYQSYGVVLAVLIAGVGLVAVAFGAARLIAPRR
                     PVGDKLLTYECGIDPVGEGWSQSQVRYYVYGFLFVIFDVESVFLFPWARIFENLGYQA
                     VVEMAIFIGILAVGLLYAWRKGVLRWA"
BASE COUNT          549 a         1007 c          986 g          453 t
ORIGIN      
        1 cgcggcgggt ccggccgtgt ccggcgccac gctcggagcc gagaccggct cctccacggg
       61 cacctccgcg ccgtcctcga tgaacggcgg cggcaggacc gagtacgtcc actcctcgag
      121 cttctccttc tcgtgcgtga gttcgacgat gtcgtactcc gagtactcga actccgggct
      181 ccagaagagc gcgtcgaacg ggcagacctc tacgcagatc ccgcagtaca tgcagagggc
      241 gtagtcgatc gcgaaccggt cgaggacctt catcgagcgc cccttgccgc cggaggcggg
      301 ctcgtgggtc tccttgtgcg cgtcgatgta gatgcaccag tccgggcact cgcgcgagca
      361 cttgtagcag acggtgcagt tcgcctcctt gagcgcgatg accccgcggg tgcgcggcgg
      421 gaggtcgggc ttctcctgtg ggtactgctg cgtgatcgag ggccggagcg catgcttgag
      481 cgtgaccgcg agccccttca tcaggccgag gccggtggag ctgggctccc gctcgggagc
      541 cctgcgaacg tctcggtcgt cgaccgccat gacccctcct agaacgcgac cttgaagacg
      601 ccgaccacca cgatctgcag cagcgcgagc gggatcagcg tgagccacga gacccgctgc
      661 agctgatcct cgcgcaggcg ggggtaggtg acgcggagcc agatgatgag gaacgagagc
      721 gccccgatct tcgcgagcat ccagagcggc tcggggatga actccaacgc cggcagcgga
      781 aggtagcccc cgaggaacag gacgctcgcg atggctgaca gggcgacgat ccctccgtac
      841 tcggcgagga ggaagaaggc gaacttcaga cccgtgtact ccgtgtaggc gccgaagatg
      901 atctcggagt ccgccaccgg catgtcgaac gggggccgcg tgagctccgc gagcgaggcc
      961 gtgaggaaga tgaggaaccc gatcggctgc gagaagacga accagtactt ctcctgggcc
     1021 tcgacgatcc cgaccatcga gagcgtctcc gcctgcatca cgatcgcggc ggccccgagc
     1081 accaagggca gctcgtaggc gatgagctgc gcggccgccc ggagggcgcc gatcagggag
     1141 aacttgttgg ccgacgccca gcccgccatg agtacgccga tcacggagac cgacgagagc
     1201 gccagcacga agaagatgcc gacgtccaga ttcaggatga agatctggtc cgagaacggg
     1261 atcacgacga acaggaggat gtacgggacg agcgcgaccg ccggcgcgag ggagaagacc
     1321 cacgcatccg ccgccgcggg gatcacgtcc tccttctgga cgaatttcgc cccgtcggcg
     1381 agcaactgcg cccatccgtg gaacctgccc gcgtacatgg gcccgagccg cccctgcatg
     1441 tgggccatca ccttgtgctc ggcgtagccg acgccgagcg gggccacgag gaagaagatc
     1501 agtacgatcg ccgccttcgt caccaggacc agccagtact cgccgaacca ctggttcacg
     1561 aagctctcgc cggcctgggc gatcactcgt cgtcctcctc ttctccttcg gccgcgcccg
     1621 gccacggctt ggcctctcgc gacatgagcg ggaagtcctt gcggagcggg tggccctcga
     1681 acggctcggg gagcagcagc gtcacgggca gcgggtgacc ctcgaacgtg acgccgaaca
     1741 tctcggtggt ctcgcgttcg tgccagttcg ccgtcggata gatgtccgag atcgtcggga
     1801 gcacggcctc ctcggccggg agccggacct tcacgcgcac ggagtgcacg tgcgtggtcg
     1861 agaacacgtg ggtgacgacc tccatgccct cgtcgccgaa gtccaccgcg gagatgaagt
     1921 cgcagtaatc gcaggcgaac tcgggctcgt tgcgcaggaa cgtcacgagc tcgcgatacc
     1981 gggacggggt caccgcgacc acgctgtgcc cgtgctggtc ctcgaaggac agcacatcgt
     2041 caccgaactg cgcccgcagg cgcgatccga cctcaggagg cggaagcggg tgtcgtgtgc
     2101 ttgccactcc acttctccct gatgtcctcg gcggcgatct tctcttggag ccggacgatc
     2161 ccctgcagca gcgcctccgg cctgggggga cagcccggaa cgtagacgtc gacgggcagg
     2221 acctggtcga tgcccttcgt gaccgagtac gagtcccagt acgggccgcc acagttggcg
     2281 cacgaaccca tcgagatcac gtacttcggc tccggcatct ggtcgtacag gcgcttgatc
     2341 accggcgtca tcttgtcggt cggcgtcccg gcgatcacga tcaggtcggc ctgcctcggc
     2401 ccgtgcgcga acgggatcac gccgaaccgg atgaagtcgt gcttggacgt cgaggccgcg
     2461 atgaactcaa tcgaacagca cgcgagtccg aagttgaaca cccacagcga gtaccgccgg
     2521 ccccagttga ggaggaactt caccggttgg atcccggcga gcggcgtctt ctcggtgatc
     2581 aggcccacct caacaccccc ttgcgccacg cgtacagcag gcccacggcc aagatcccga
     2641 tgaagatcgc catctcgacg accgcctggt acccgaggtt ctcgaagatc ctggcccacg
     2701 ggaacaggaa gacggactcg acgtcgaaga tcacgaacag gaacccgtag acgtaatagc
     2761 ggacctggct ctgtgaccaa ccctcgccga ccgggtcgat cccgcactcg tacgtgagca
     2821 gcttgtcgcc gaccggccgc cggggtgcga tgagccttgc ggcgccgaac gccaccgcga
     2881 cgagccccac gccggcgatc aggaccgcga ggacgacccc gtacgactgg tagtaggtct
     2941 ccacgcttgt gaatgttttc ccgagcagcg cggattctag caagcgtccc ccgcc
//
LOCUS       DATBRK010000318         4239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1500130, whole genome shotgun sequence.
ACCESSION   DATBRK010000318 DATBRK010000000
VERSION     DATBRK010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500130"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VIH70_09485"
     CDS             complement(<1..269)
                     /locus_tag="VIH70_09485"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8116633.1"
                     /translation="MCAFDGEHVVVIGAGVAGSAAARVLLAEGATVRVSDSGRATPGA
                     GDLRAEGIEVLEGGHALDHLDGATMVVASPGVPPHAEILRWAHDR"
     gene            complement(269..1324)
                     /gene="mraY"
                     /locus_tag="VIH70_09490"
     CDS             complement(269..1324)
                     /gene="mraY"
                     /locus_tag="VIH70_09490"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY8116634.1"
                     /translation="MRQILIAAAVGLSVTLLGTPIAIRLFRLWGWGQRIREDGPHTHL
                     EKMGTPTMGGTVIIAGIVAGYVATRIVFTTVTAAGISVLLATVLLGMLGFMDDFIKVR
                     RRRSLGLSKTAKIAGQAVIALIFGMVAVRAAGVPTELTFIRATGVQLGLLFYLWVFVI
                     LAASSNAVNLTDGLDGLASGASILVLSAFVFIAFWQFRHTCEMIAGAACYPIDVNAAL
                     DLAIVAGAGAGATAGFLWWNAAPARIFMGDTGSLALGGLMGALAIVTNTQLLLVVLGG
                     LFVIETASVIAQVVAFRGFERRIFRMSPIHHHFELAGWPEFTVIVRFWIVTGLCVAVG
                     LGLFYTDFISRGGLGLE"
     gene            complement(1356..2717)
                     /gene="murF"
                     /locus_tag="VIH70_09495"
     CDS             complement(1356..2717)
                     /gene="murF"
                     /locus_tag="VIH70_09495"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY8116635.1"
                     /translation="MIERRLSEIASAVHGTLRGPDAVASSVETDSRAVGPGGLFVAID
                     GERLDGHAFVGDAVARGAVAALVHRSGIDGTVIEVPHTGRALLDLAADERERMTGTRV
                     VGITGSNGKTSAKDLAAAVVGTRLRTHASPASFNNEVGLPMTLLGADRGVEVVVAEMG
                     ARREGDVRLLCDVARPDAVVVTNVGTAHLGIFGSWDAIVRAGSEPVEWLGADGTAIVN
                     ADDPVARAYADETDAAVITFGLDPGADVRAEDVELGPDARATFTMWSAGEREPVGLAV
                     PGDHMVSNALAAAAVGRWAGVTLAECATALKGAGIGHWRMETSTGSSGIVIVNDAYNA
                     NPESMAAALKSARWMAKGERLVAVLGHMAELGPVEVEEHEKLGRLVVRIGVERLITVG
                     ERARTIARAAIREGQLPEDVSSYEDPSDAAADVREWARPGDVVLVKGSRVAGLERVAE
                     ALR"
     gene            complement(2714..4204)
                     /locus_tag="VIH70_09500"
     CDS             complement(2714..4204)
                     /locus_tag="VIH70_09500"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY8116636.1"
                     /translation="MAPPRSLTLHDLVRDLPEAELRGGADVLVSSLCYRSDDARPGAL
                     FFCVPGRTSDGHAFAGDAVSRGSTGIVVDRWLELDVPQVKVPDVREAMGPISAAFYGR
                     PADAMTVVGVTGTNGKTTTTFLLEAIFRADGRTPGVIGTTGVRIDGRPEPFPRTTPEA
                     PDLQRLVATMADEGVTAVAMEVSSHGLHQHRVDGIRYSVAVFTNLTQDHLDYHSSMKE
                     YFLAKSRLFTPAMSDRAVVNLDDERGRDLAGSGLPTITYGLAPESDVRATDVESTAEG
                     IGFLADGVPIRSALRGSFNVENALAAFATARSLGIEDGVAADAISSVRVVPGRVETVE
                     VGQGFLVLVDYAHTPDSVENVLRAARSLTDGRLIVVLGAGGDRDRVKRPLMGRAATSN
                     ADLAVITSDNPRSEDPLEIIAEITPGAERGAEEGGGRFVVRPDRRAAIRLAMEEASPG
                     DVVVIAGKGHETYQELADRRIPFDDRAVAEDELRSIGKAAPIGTAP"
BASE COUNT          650 a         1606 c         1413 g          570 t
ORIGIN      
        1 ctccgatcgt gggcccacct caggatctcg gcgtgcggcg gcacccccgg gctcgcgacg
       61 accatcgtcg cgccgtcgag atggtcgagc gcgtgcccac cctcgagcac ctcgatcccc
      121 tccgcacgca ggtccccggc gccgggcgtc gcgcgaccgg aatcgctcac gcggaccgtc
      181 gcgccctccg cgagcaggac ccgcgccgcc gccgagccgg ccacaccggc gccgatcacg
      241 acgacgtgtt cgccgtcgaa cgcacacatc actcgagccc cagtccgccg cggctgatga
      301 agtccgtata gaacaacccg agccccaccg cgacgcacag accggtcacg atccagaacc
      361 gcacgatcac cgtgaactcg ggccagccgg cgagctcgaa gtggtggtgg atcggcgaca
      421 tccggaagat ccgccgctcg aagccgcgga acgcgaccac ctgcgcgatc accgacgccg
      481 tctcgatcac gaacagcccg cctaggacga ccagcagcaa ctgggtgttc gtcacgatcg
      541 cgagcgcccc catcagcccg ccgagggcca gcgagccggt atcccccatg aagattcggg
      601 ccggcgcggc gttccaccac aggaaccctg cggtcgcgcc ggctccggcc ccggccacga
      661 tcgcgagatc gagcgcggcg ttgacgtcga tcgggtagca ggcggcgccc gcgatcatct
      721 cgcacgtgtg ccggaactgc cagaacgcga tgaagacgaa cgcggagagc acgaggatcg
      781 acgcaccgga ggcgaggccg tcgagcccgt ccgtcaggtt cacggcgttc gaggacgccg
      841 cgaggatcac gaacacccag aggtagaaca ggagcccgag ctggacgccg gtggcacgga
      901 tgaaggtgag ctcggtcggc acccccgccg cccggacggc caccatgccg aagatcaggg
      961 cgatcacggc ctggcccgcg atcttcgcgg tcttcgacag gccgagcgac cgccggcgac
     1021 gcaccttgat gaagtcgtcc atgaaaccga gcatcccgag gagcacggtc gccagcagca
     1081 ccgagatccc ggcggcggtg acggtcgtga acacgatgcg ggtcgcgacg tagcccgcga
     1141 cgatccccgc gatgatcacg gtgccaccca tcgtcggcgt gcccatcttc tccaggtggg
     1201 tgtgcggccc gtcctcgcgg atccgctgac cccaccccca cagtcggaac agccggatcg
     1261 cgatcggtgt gccgagcaac gtcaccgaca ggcccaccgc cgcggcgatc aggatctgtc
     1321 tcacgggcgc gcctcgctgc gttcgcgacc cggcctcatc gcagggcctc cgccacgcgt
     1381 tccagacccg ccacccggga ccccttcacg agcacgacgt caccgggcct cgcccattcg
     1441 cggacatcgg cggcggcgtc cgatgggtcc tcgtacgacg agacgtcctc gggaagctgc
     1501 ccctcgcgga tcgccgcgcg ggcgatcgtt cgcgcccgct cccccacggt gatcagccgt
     1561 tcgacgccga tcctgaccac gagccgcccc agcttctcgt gctcctcgac ctcgaccggc
     1621 ccgagctcgg ccatgtgccc gagtaccgcc accagacgct cgcccttggc catccaacga
     1681 gcggacttca acgcggccgc catcgactcg gggttcgcgt tgtacgcgtc gttcacgatc
     1741 acgatccccg acgaaccggt ggaggtctcc atccgccagt gcccgatgcc cgcccccttc
     1801 agcgccgtcg cgcactcggc gagggtcaca ccggcccagc gcccgaccgc cgcggctgcg
     1861 agggcgttcg acaccatgtg gtcgccgggg accgcgagcc ccaccggctc gcgctcgccg
     1921 gccgaccaca tggtgaacgt ggctcgcgca tcgggaccga gctcgacgtc ctcggcccgg
     1981 acatcggcgc ccggatccag gccgaaggtg atcaccgccg cgtcggtctc gtcggcgtac
     2041 gcgcgcgcga cgggatcgtc cgcgttcacg atcgccgtgc cgtccgcacc gagccactcg
     2101 accggctcgg accccgcgcg gacgatcgcg tcccacgacc cgaagatccc caggtgcgcc
     2161 gtcccgacgt tcgtcacgac gacggcgtcg ggccgggcga cgtcgcagag caaccgaacg
     2221 tcaccctcgc gtcgcgcgcc catctccgcg accacgacct cgacaccgcg atcggcgccg
     2281 agcagcgtca tcgggagccc gacctcgttg ttgaacgacg ccgggctggc gtgggtgcgc
     2341 agacgcgtgc ccactaccgc ggcggcgaga tccttggcag acgtcttgcc gttcgagccg
     2401 gtgatgccga ccacgcgggt ccccgtcatc cgttcgcgct cgtcggccgc gaggtcgagc
     2461 agggcgcgcc cggtatgcgg aacctcgatc accgtcccgt cgatcccgga tcgatgcacg
     2521 agcgcggcga cggctccgcg cgcgaccgcg tcgccgacga acgcgtgacc atccaggcgc
     2581 tccccatcga tcgcgacgaa caggcctccc ggcccgacgg cgcgcgagtc cgtctccact
     2641 gaggacgcga cggcgtcggg tccgcggagc gtgccgtgga cggccgacgc gatctccgag
     2701 agccggcgtt cgatcacggg gccgtcccga tcggcgccgc cttcccgatc gatcggagct
     2761 catcctcggc taccgctcgg tcgtcgaacg ggatcctccg gtccgcgagc tcctggtagg
     2821 tctcgtggcc cttgccggcg atcacgacga cgtcgccggg tgaggcctcc tccatcgcga
     2881 gtcggatcgc ggcccgccga tccggtcgga cgacgaaacg gccgcctccc tcctccgctc
     2941 cgcgttcggc gcccggcgtg atctcggcga tgatctcgag cgggtcctcg ctgcgaggat
     3001 tgtcgctcgt gatgaccgcg aggtcggcgt tcgacgtggc cgcgcggccc atgagtggtc
     3061 gcttgacacg gtcccggtcg ccgccggccc cgagcaccac gatcagccgg ccgtccgtga
     3121 gcgatcgggc cgcgcgcaac acgttctcca cgctgtcggg cgtatgggcg tagtcgacca
     3181 acacgaggaa tccctggccc acctcgaccg tctccacgcg cccggggacg acgcggaccg
     3241 acgagatcgc gtccgccgcg acgccgtcct cgatcccgag cgatcgcgcg gtggcgaagg
     3301 cggccagggc gttctcgacg ttgaacgacc ctcggagcgc cgaacggatc gggaccccat
     3361 cggcgaggaa gccgatcccc tcggccgtcg actccacgtc cgtggctcgc acgtccgact
     3421 cgggcgcgag cccgtacgtg atcgtcggca gacccgagcc ggccagatct cggccccgct
     3481 cgtcgtcgag gttcacgacg gcgcgatcgc tcatcgccgg cgtgaacagc cgcgatttgg
     3541 cgaggaagta ctccttcatc gacgagtggt agtccaggtg atcctgtgtg aggttggtga
     3601 acacggcgac cgagtaccgg atgccgtcga cacggtgctg atgcagccca tgcgatgaga
     3661 cctccatcgc gaccgcggtc acgccctcgt cggccatcgt ggccacgagc cgctgcaggt
     3721 ccggcgcctc gggcgtcgtg cgcgggaagg gctcgggcct gccgtcgatc cgcacaccgg
     3781 tggtgccgat cacgcccggg gtccggccgt ccgcccggaa gatcgcctcc agcaggaagg
     3841 tcgtcgtggt cttcccgttc gtgccggtca cgccgacgac ggtcatcgca tccgccggcc
     3901 gcccgtagaa cgcggccgag atggggccca tcgcctcgcg cacgtccggc accttcacct
     3961 ggggcacgtc gagctcgagc cagcggtcga cgacgatgcc cgtcgacccg cgcgagaccg
     4021 cgtcgccggc gaaggcatga ccgtcggagg ttctcccggg gacgcagaag aagagcgcgc
     4081 cgggccgggc gtcgtccgag cggtagcaga ggctcgagac gagcacgtcc gcgccgcctc
     4141 ggagctcggc ctcggggagg tcccggacga ggtcatggag cgtcaacgag cggggagggg
     4201 ccacggttcc tgagcctatc gcctgcgtgg cggcgcctt
//
LOCUS       DATBRK010000319         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1644865, whole genome shotgun sequence.
ACCESSION   DATBRK010000319 DATBRK010000000
VERSION     DATBRK010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644865"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1744
                     /locus_tag="VIH70_09505"
     CDS             <1..>1744
                     /locus_tag="VIH70_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116637.1"
                     /translation="AELFGGGMTLATGTVEVDAEIRRLLHMDLQVFRASVYAEQKQLD
                     AFSDVTAGKRKEMALRLLGIRPVEDARNASRREARATRESADQLTGAVPDVAALEADL
                     KAVKDVVTEAKRLAKAAATELRQAVAAERAVKKAFTGSDAVRQRIEKLTIELRAKTEQ
                     RDHAAEQHRALVERVERMNAAVAELPAIDDELAGLAGIDDRLRLGTALVERGDELERT
                     QARLDAMPQTDRATVLSELEAASGAVERARSAFAGAEAEHAHRSTLLEQALERLERAA
                     EADPSQPCPTCGRPMGDDFASYVKHCKAEVAGAKKTAATAAAAVRKALAERARAEKDQ
                     VTAAEAGERAREQDAHRSQLAERIGSLSAEVATLAEPFDGAVPELEELRASAERARAL
                     GTRAAELRTERQHLSQAERDLDDALRRIQLLDGELAKLAGEAEGLAFDDAAHKRLHDE
                     LEEAIAALDQAREAERLASDALKDAEKAEAGLVAAVRQAKETQARLDELRSDARYAER
                     VALLLEGFRNHLVARIGPELSREAEALFRELTNHEYDDLKVDEEKLTIQIADGDSYFA
                     IDRFSGSETDLANLA"
BASE COUNT          288 a          589 c          676 g          191 t
ORIGIN      
        1 ggccgagctg ttcggcgggg gcatgacgct cgcgaccggg accgtcgagg tcgacgcgga
       61 gatccgccgc ctcctccaca tggacctgca ggtcttccgc gcctcggtgt acgccgagca
      121 gaagcagctc gacgcgttct cggacgtgac cgcgggcaag cgcaaggaga tggcgctgcg
      181 actgctgggg atccgcccgg tcgaggacgc acggaacgca tcgcggcggg aggcgcgggc
      241 gaccagggag agcgcggacc agctcaccgg cgccgtgccc gacgtcgccg cgctcgaggc
      301 cgacctcaag gccgtcaagg acgtggtgac ggaagccaag cgtctcgcga aagccgcggc
      361 caccgagctg aggcaggcgg tcgccgccga gagggccgtg aagaaggcgt tcaccgggtc
      421 ggacgccgtc cgccaacgca tcgagaagct gacgatcgag ctccgcgcga agaccgaaca
      481 gcgcgaccac gcggcggagc agcaccgggc tctggtcgag cgcgtcgagc ggatgaacgc
      541 cgcggtcgcg gagctgcccg cgatcgacga cgagctcgcg ggactggccg ggatcgacga
      601 tcggctgcgg ctcggcaccg ccctggtcga acggggggac gagctcgagc ggacccaggc
      661 gcggctggac gcgatgcccc agaccgaccg cgcgaccgtg ctctcggagc tcgaggccgc
      721 gtcgggcgcg gtcgaacggg ctcggtccgc cttcgccggt gccgaggccg aacatgcaca
      781 ccggtcgacc ctcctggagc aggccctcga acggctcgag cgcgcggcgg aggccgatcc
      841 gtcccagccc tgccccacct gcggccgccc gatgggtgac gacttcgcgt cgtacgtgaa
      901 gcactgcaag gcggaggtcg cgggcgcgaa gaagaccgcg gccaccgccg cggcggcggt
      961 caggaaggcg ctcgccgagc gagctcgggc ggagaaggac caggtcaccg cggcggaggc
     1021 gggggaacgg gcgcgtgaac aggacgcgca ccggtcgcag ctcgccgagc ggatcgggtc
     1081 gctctcggcc gaggtggcga cgctggcgga gccgttcgat ggggccgtcc ccgagctcga
     1141 ggagcttcgg gcgagcgccg agcgcgcgcg agccctcggc acccgcgcgg cggagctgcg
     1201 gaccgaacgc cagcacctct cgcaggccga acgcgacctg gacgacgcgc tgcgacggat
     1261 ccaactcctc gacggcgagc tcgcgaagct cgcgggggag gccgaggggc tcgcgtttga
     1321 cgacgcggcg cataagcgct tgcacgacga gctcgaggaa gcgatcgccg cgctggacca
     1381 ggcgcgcgag gccgaacgct tggcgagcga cgccctgaag gacgccgaga aggcggaggc
     1441 cgggctcgtc gccgcggtgc gtcaggccaa ggagacgcag gccaggttgg acgagctgcg
     1501 ttcggacgcg cggtacgcgg agcgggtggc gctgttgctc gaggggttcc gcaaccatct
     1561 ggtggcgcgc atcgggcccg agctctcccg cgaggcggag gcactgttcc gcgagctcac
     1621 gaaccacgag tacgacgacc tcaaggtcga cgaggagaag ctcacgatcc agatcgccga
     1681 cggggactcg tacttcgcga tcgatcgctt ctcgggttcg gagaccgatc tggcgaacct
     1741 cgcg
//
LOCUS       DATBRK010000320        10035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_919742, whole genome shotgun sequence.
ACCESSION   DATBRK010000320 DATBRK010000000
VERSION     DATBRK010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10035
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919742"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VIH70_09510"
     CDS             complement(<1..1329)
                     /locus_tag="VIH70_09510"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY8116638.1"
                     /translation="MRLSALFAPTLRDDPAEAEIASHRLLLRAAFVRQVAAGVYTTLP
                     LGLRSIRKIERIVREEMNASGAQEFRMPILLPAEPWKATGRWDLYGDTLFRLRDRHER
                     ELLLGPTQEEVVTGLVARDLPSYRDLPVNLYQIEWKYRDEFRPRFGLLRGREFLMKDA
                     YTFDRDEEGMAAAYDVMLRAYRRVFDRCGLTYIVVDAEPGQIGGGVNHEFMARADVGE
                     DLFVECVNGDYLADWKAATPRPPDPASGDGAAPLEKVHTPDAATIDAVSELLDVPADR
                     TLKTMLFDAGGETVAVLVPGDREVEEAKIERLLFPAPVRRFEDADFAARGFAKGYVGP
                     QGLGEEVTILADHSVRGGGDWVTGANEADHHVTGANVTRDFRVDRYEDVIALRDGDRC
                     PIDGGELRIGRSIVVGHIYQLGTRYSAPLRATFVDEDGSERLYQMGSHGIG"
     gene            complement(1361..2947)
                     /locus_tag="VIH70_09515"
     CDS             complement(1361..2947)
                     /locus_tag="VIH70_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116639.1"
                     /translation="MRTSSRLICVLTGLALAVGVLAFPAAAGHEGRRLGRTATRAIVY
                     GRDAGVSSRKATSFDVFGRRSRPFVSASGAAGASSPLLPGLVGVGFDALADPSSKPSD
                     TTGALGDGFYVAAVNTEMAVYDRNGAQVVAPIPLDQLHANSTGLFVFDPKVVYDQYLD
                     TFLLVYLVQEDLPRLSRIVTVAIPNATASDPSTWCAVSFKGDQVSGGTAVWADYPGVG
                     FNETHVTITTNQFTFPTTIGRFRYSQVMTIDKVGLYDCNQPAPSPTVFAGTKTQDDKG
                     HQAFTLRPAETVGSSPGPQLLTSFQPAKGKRDYLTIWRIKPTASGFALKKASKRTGKV
                     SIPPLGTQGGGSPNDPDLWWDAGDHRFINAFYDADQNQLFTAHAVFRNLEPDTLTGGY
                     PEAAVRWYEIDPAAILKNSVVARRGIVGAPEVDVGWPTVATDSTGTLFVTYSRASAPQ
                     GEFLSAWVATIPPGSTAATQLLVQAGLSTYDAIQGPERWGDFTAINRDPFDGQNVATF
                     NQYASSSTQWQQFIGVVTDI"
     gene            3100..4587
                     /locus_tag="VIH70_09520"
     CDS             3100..4587
                     /locus_tag="VIH70_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEY8116640.1"
                     /translation="MRVRPDRGTFERSAQGFALAPVWTELLADVSTPVGVFPALAGDG
                     PGILLESVERSERWGRYSFVAGDPAAIVVLDREGLRNLAATDRLSVEMPNTGDARADL
                     VALASSLRAPRVADLPSLTGGLMGFLSYEAAELLDGHPFPSGDTLGPPIALMLADRAV
                     VFDHWRQRLLLVAHVAPDAYDQGAEALEDLAERVSAPNAPSPEPLPGTMAVVDSVGEP
                     NMPDDRYREIVRTMKDHISAGDIYQGVPSRRLGFPCTGGGFPVYRRLRVTNPAPYMFF
                     VRMLGLELAGSSPEPLVRVEGRTVTSRPIAGTRPRGETELRDRLYEHELLADPKERAE
                     HAMLVDLARNDLGRVCTPGTVRPSELLSVERFSKVMHIVSTVEGELAEGAHPLDALAV
                     TFPAGTVTGAPKRRAMELIAELEPTARGPYAGAVGYCTFAGDLDFCITIRTAVVADGA
                     AYVQTGAGVVADSDPDVELAETNAKAAALLPAIVEAEASGVWEDP"
     gene            4584..5150
                     /locus_tag="VIH70_09525"
     CDS             4584..5150
                     /locus_tag="VIH70_09525"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY8116641.1"
                     /translation="MILVVDHYDSFTYNLVQLLETHGTTTEVVKSDAETAEALVARRP
                     EAVVLSPGPGRPEDAGCFTELLRILPASTPVLGVCLGHQAIGVVAGARVERATPVHGK
                     ASLVHHDGTGIFVDVPNPFEAGRYHSLVVTREGFPDELELTAWSDDDLVMAARHRSLP
                     RIGVQFHPESILTPDGPTIVRNFLSILG"
     gene            complement(5194..6285)
                     /gene="ispG"
                     /locus_tag="VIH70_09530"
                     /gene_synonym="gcpE"
     CDS             complement(5194..6285)
                     /gene="ispG"
                     /locus_tag="VIH70_09530"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007501661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY8116642.1"
                     /translation="MLTERRKTRQVRVGDVTVGGDAPIRVQSMTTTKTADINATLQQI
                     ASLVAAGVDIVRVAVPHWEDAEALTAIAAKSTVPVVADIHFQWKYAMAALEAGIQGLR
                     INPGNIKFQDKVRVIAREARARGVPIRIGVNAGSLEKDVLARHGAPTAEALVESALYE
                     ARILEEEDFFDIKISVKHSNAGVMIQAYRLLAQACDYPLHLGVTEAGPMPAGGVKSAV
                     GIGALLSEGIGDTIRVSLTDDPVEEVKVGTWILQSLGLRKRGLDLVACPSCGRAEVDV
                     LGLTKQVQQAIEREGLKVPIRVAVMGCVVNGPGEAREADLGIAAGKGQGFLIVKGEVV
                     DKIPEDRFVERVIEEARKIAVAREAEAET"
     gene            complement(6337..6786)
                     /locus_tag="VIH70_09535"
     CDS             complement(6337..6786)
                     /locus_tag="VIH70_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY8116643.1"
                     /translation="MTSATTSIEVAAPERYAFLDLTEELERAIKDSGVTDGSVVAFCP
                     HTTCALIVNEWEDGALEDFRARILRLVPDDVYHAHDDATRRTQNLDESHERRNGPAHV
                     KAILLSASSHAIPVAGGTAMLGRWQRLILFEMDEPKDRQVVFHVFGE"
     gene            complement(6783..7943)
                     /locus_tag="VIH70_09540"
     CDS             complement(6783..7943)
                     /locus_tag="VIH70_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8116644.1"
                     /translation="MTLLQRLPTIRSKLGSVIVFAVGMTLLLVYLILGVGFEVFTIGE
                     HWLELLLAGVLGAGIALVIARWVARGMTQPLRDMARAARRMETGDYSQRIHTDSRDEV
                     GRLAEAFNRMSTELGGLELLRRELVANVSHELKTPISALRAHLENLLDGVEQPDPETL
                     QIMLLQSERLGRLVDQLLDLSRLESGDVPLERERVELAPLVSQVFSEILVARPDPGVT
                     LDDDLPGALPPVFADRERVHQVLFNLLDNAVRFTPTGGRVTVTASNRNGSIDVVVADT
                     GPGIPAEHLPHVFERFYRVDESRSRDDGGTGIGLAIARSVVEAHGGRIWADSEPGHGS
                     AFTFELPVASPSGDPGGERSSTVPQKETSRKETSQKETSQKETSQKETRRNE"
     gene            complement(7951..8643)
                     /locus_tag="VIH70_09545"
     CDS             complement(7951..8643)
                     /locus_tag="VIH70_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007453681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8116645.1"
                     /translation="MVEQTVLVVDDETAIAEAVRARLSSEGFRVLVAADGPQALELAS
                     AEEPDLVVLDLMLPGMDGLEVCRRLQAGRWVPVLMLTARTEEADKVAGFAVGADDYLT
                     KPFSLRELTVRVRAILRRVERITSPPATEPIDHGGLSVDPAKRKVNVDGEEVQLTPLE
                     FEILLALAREPGVVLSREQLMDRVWGYRDYAGGRVVDSHVARIRRKLGEDGAEPRFVR
                     TVHGVGYAFGDA"
     gene            complement(8758..9879)
                     /locus_tag="VIH70_09550"
     CDS             complement(8758..9879)
                     /locus_tag="VIH70_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY8116646.1"
                     /translation="MAALGIGAFIVALILVIVIHEAAHFAVARAFGIKVSEFFVGFGP
                     KLWSRMSGETEVGLKAIPAGGYVKIAGMNPYETVAPADLPRTFGAKPIWQRALVILAG
                     PATHFVLAFLCFALWLGLVGQPIARGVVISAVKPTLHGVESPAAAAGLRSGDVVVVVD
                     DLADPSADELVAYTTAHAGEPVTFTIRRDGETFRAVMVPALSQVGGEVIGRVGVGVQP
                     RTVVRGTQGVIGSVTGAADLVGDNIVGTVHGVARIFGPEGIGRLATLIFTDEPRRVED
                     PASVVGIGRVAGSLASDDRFGDLLLLFGVVNVFVGLLNLLPLPPFDGGHLAVLAIEKI
                     RGRPVDMRKVIPVSAAVAAFFIVFTVAVVYVDLVKPPSV"
BASE COUNT         1510 a         3608 c         3384 g         1533 t
ORIGIN      
        1 gccgatcccg tggctcccca tctggtagag ccgctccgaa ccgtcttcgt cgacgaacgt
       61 cgcccgcagc ggggccgagt accgcgtccc gagctgatag atgtggccca cgacgatcga
      121 acggccgatc cggagctcgc caccgtcgat cgggcagcgg tcgccgtcac gcagggcgat
      181 cacgtcctcg tatcgatcga cgcggaagtc cctggtgacg ttcgcaccgg tgacgtggtg
      241 gtccgcctcg ttggccccgg tcacccagtc gccgccgccg cgcaccgagt gatcggcgag
      301 gatcgtcacc tcctccccca gaccctgcgg accgacgtag cccttcgcga atccccgggc
      361 ggcgaagtcg gcgtcctcga agcggcggac cggggccggg aacaagagcc gctcgatctt
      421 cgcctcctcg acctcccggt cgcccggcac caggaccgcg accgtctccc cgccggcgtc
      481 gaacagcatc gtcttcagcg tgcggtcggc cggtacgtcg agcagctccg agaccgcgtc
      541 gatcgtcgcg gcgtccggcg tgtgaacctt ctcgagcggc gcggctccgt cgccggaggc
      601 agggtcgggc ggccgagggg tggcggcctt ccagtcggcc agatagtcgc cgttcacgca
      661 ctcgacgaac aggtcctcgc cgacgtccgc gcgagccatg aactcgtggt tcacgccgcc
      721 gccgatctgg cccggctcgg cgtcgaccac gatgtacgtc aacccgcatc ggtcgaagac
      781 cctccggtat gcccgcagca tcacgtcgta ggccgcggcc atgccctcct cgtcgcggtc
      841 gaacgtgtag gcgtccttca tcaggaactc gcgaccgcgc aggagcccga atcgcgggcg
      901 gaactcgtcc cggtacttcc actcgatctg gtacaggttc acgggcaggt ctcggtacga
      961 ggggaggtcc cgggcgacga gtccggtcac gacctcctcc tgcgtgggac ccagcagcag
     1021 ctcgcgctcg tgacggtccc ggagacggaa cagcgtgtct ccgtacaggt cccaccggcc
     1081 cgtcgccttc cacggttcgg ccggcagcag gatcggcatc cggaactcct gcgcgcccga
     1141 cgcgttcatc tcctcgcgga cgatccgttc gatcttgcgg atcgaccgga gaccgagcgg
     1201 caacgtcgtg tagacgcccg cggcgacctg cctcacgaac gccgcgcgca ggaggagccg
     1261 atgcgacgcg atctccgcct cggcaggatc gtcacgcagc gtcggcgcga acagagccga
     1321 gagcctcatg gagggtcagg ataccggtcg cactcagccg ctagatgtcg gtgacgacgc
     1381 cgatgaactg ctgccactgg gtcgaggatg acgcgtactg attgaaggtc gcgacgttct
     1441 gaccgtcgaa tgggtcgcgg ttgatcgcgg tgaagtcgcc ccagcgctcg gggccctgga
     1501 tggcgtcgta ggtcgacaac ccagcctgca cgaggagctg cgtcgcggcg gtcgaccccg
     1561 gcgggatcgt ggcgacccag gcggagagga actctccctg cggggcgctc gcccggctgt
     1621 acgtcacgaa gagcgttccg gtggagtcgg tcgccacggt cggccatccc acatccacct
     1681 ccggcgcgcc gacgatcccc ctgcgcgcca cgacggagtt cttgaggatc gcggccggat
     1741 cgatctcgta ccagcggacg gcggcctcgg ggtagccccc cgtcagggtg tctggctcga
     1801 ggttcctgaa gaccgcatgc gcggtgaaga gttggttctg atcggcgtcg tagaaggcat
     1861 tgatgaagcg gtgatctccc gcgtcccacc agagatcggg gtcgttcgga ctacctccgc
     1921 cctgagtccc cagcggcggg atcgagacct tcccggtgcg cttggaagcc ttcttcaggg
     1981 cgaagccgga cgccgttggc ttgatccgcc agatcgtcaa gtagtcgcgc ttccccttcg
     2041 cgggttggaa cgaggtcagc agctgcgggc cgggcgagga gcccacggtc tcggccggtc
     2101 gcagcgtgaa ggcctggtga cccttgtcgt cctgggtctt cgttccggcg aacaccgtcg
     2161 gcgacggcgc cggctggttg cagtcgtaca gccccacctt gtcgatcgtc atcacctgcg
     2221 agtagcgaaa ccgaccgatc gtggtcggga aggtgaactg gttcgtggtg atcgtgacgt
     2281 gcgtctcgtt gaaacccacc cccggatagt cggcccacac cgcggtcccc ccggagacct
     2341 ggtccccctt gaacgacacc gcgcaccagg tactgggatc gctcgccgtc gcgttcggga
     2401 tcgcgaccgt cacgatccga gacagacggg gcaagtcctc ctgcaccaga tacacgagga
     2461 ggaacgtgtc gaggtactgg tcgtagacca ccttggggtc gaagacgaac aggccagtcg
     2521 aattcgcgtg aagctggtcg agcgggatgg gcgcgacgac ttgagcgccg ttccggtcgt
     2581 agacggccat ctcggtgttg acggcggcga cgtagaagcc gtcccccagg gcgccggtcg
     2641 tgtcggatgg cttggacgag ggatcggcca gcgcgtcgaa cccgaccccg acaaggcccg
     2701 gtagcagtgg gctcgatgct cccgccgcgc cggacgccga gacgaacggc cgagaccggc
     2761 gcccgaacac gtcgaacgac gtcgccttgc ggctcgacac gcccgcgtcc ctcccgtaca
     2821 cgatcgctcg agtcgcggtg cggcccagac gtcgcccctc atgccccgcc gcggccggga
     2881 aggcgagcac gccgacggcc agcgcgagac ccgtcaggac gcagatcaac ctgctggacg
     2941 ttcgcatcga cgctcccccc cttgctcggt ggcgaaacgg taccccacgt ccgggagccc
     3001 agtcgaccca tgggcagacg atctggaact cctcccggcc ggtcgcgggt agggtacggc
     3061 tgcttctcag ggacggcggc atggcgcgaa ggagatcagg tgagggtccg gcccgatcga
     3121 ggcacgttcg aacgctcggc ccagggcttc gcgctcgcac cggtgtggac cgagctgctg
     3181 gccgacgtct ccacgccggt cggcgtcttc ccggcgctgg ccggggatgg gcccggcatc
     3241 ctgctcgaga gcgtcgagcg gtccgagcgc tgggggcggt attcgttcgt cgccggcgat
     3301 cccgccgcga tcgtcgtgct cgaccgggag ggcctccgga acctcgccgc caccgaccgg
     3361 ctttcggtcg agatgccgaa caccggcgac gcgcgggccg acctcgttgc cttggcctcg
     3421 tcgctccggg cgccgcgggt cgccgacctc ccctcgctca ccggcggcct gatgggcttc
     3481 ctctcctacg aggcggcgga gctcctcgac gggcacccgt ttccgagcgg cgataccctc
     3541 gggcccccga tcgcgctgat gttggccgac cgcgcggtcg tgttcgacca ctggcggcag
     3601 cgcctgctcc tggttgcgca cgtcgccccg gacgcctacg accagggcgc agaggcgctg
     3661 gaggatctgg cagaacgggt ctcggccccg aacgctccgt caccggagcc cctgccggga
     3721 accatggccg tcgtcgattc cgtgggagaa cccaacatgc ccgacgaccg ctaccgcgag
     3781 atcgtcagga cgatgaagga ccacatctcg gcgggcgaca tctaccaggg cgtgccctcg
     3841 cgccggctcg gattcccgtg caccggcggc gggttccccg tctaccggag gctccgcgtc
     3901 acgaacccgg ctccctacat gttcttcgtc cggatgctgg gactcgagct cgccggctcg
     3961 tcacccgaac cactggtgcg cgtggaggga cggacggtca cgagccgtcc gatcgccggc
     4021 acgcgccctc ggggcgagac cgagctccgg gaccgcctgt acgagcacga gctgctcgcc
     4081 gacccgaagg agcgtgcgga gcacgcgatg ctcgtcgacc tcgcccgcaa cgatctcggc
     4141 cgcgtctgca cccccgggac cgttcgcccg agcgagctct tgtcggtcga gcgcttctcg
     4201 aaggtcatgc acatcgtgag cacggtcgag ggcgagctcg ccgagggcgc ccacccgctc
     4261 gacgcgctcg cggtgacgtt ccccgcgggg acggtgacgg gcgcgccgaa gcgtcgcgcg
     4321 atggaactga tcgccgagct cgagccgacc gctcgcggtc cgtacgccgg ggcggtcggc
     4381 tactgcacgt tcgcgggcga cctcgacttc tgcatcacga tccggacggc ggtcgtcgcc
     4441 gacggcgccg catacgtcca gacgggcgcg ggggtcgtcg ctgactccga ccccgacgtc
     4501 gagctggccg agaccaacgc gaaggccgcg gccctgctgc ccgcgatcgt cgaggcggag
     4561 gcatccggtg tatgggagga cccgtgatcc tggtcgtcga ccactacgac tcgttcacgt
     4621 acaacctcgt gcagctactc gagacccacg gaacgacgac cgaggtcgtg aagagcgacg
     4681 ccgagaccgc ggaggccctc gtcgcgcgcc gaccggaggc cgtcgtcctg tcgccgggcc
     4741 ccggccggcc cgaggacgcg gggtgtttca ccgagctgct gcggatcctc ccggcgtcca
     4801 cgccggtgct cggcgtgtgc ctcggccatc aggcgatagg cgtggtcgcc ggcgctcgcg
     4861 tcgaacgcgc gacgccggtg cacggcaagg cgtcgctcgt ccaccacgac ggcacgggta
     4921 tcttcgtgga cgtccccaac ccgttcgagg ccggcaggta ccactcgctg gtcgtgaccc
     4981 gggagggctt ccccgacgag ctcgaactga ccgcgtggag cgacgacgac ctcgtgatgg
     5041 cggcccggca tcggtcgctg ccgaggatcg gggtgcagtt ccaccccgaa tcgatcctga
     5101 cgccggacgg ccccacgatc gtccggaact ttctgtccat cctggggtag tgccccgagc
     5161 gcgcctacgc tcgctgcgta gacggggcgc gacctacgtc tcggcctccg cctcgcgcgc
     5221 gaccgcgatc ttccgggcct cctcgatgac ccgttcgacg aaccggtctt cggggatctt
     5281 gtcgaccacc tcacccttga cgatgaggaa cccctggccc ttgccggcag cgatcccgag
     5341 atcggcctcc cgagcctcgc ccggtccgtt cacgacgcac cccatcacgg cgacccggat
     5401 ggggaccttc agcccctcgc gttcgatcgc ctgctgcacc tgcttggtga ggccgaggac
     5461 gtcgacctcc gcccgtccgc aggacggaca cgcgacgagg tcgagtccgc gcttgcgcag
     5521 gcccagcgac tgcaggatcc acgtcccgac cttgacttcc tcgaccgggt cgtccgtgag
     5581 cgagacgcgg atcgtgtccc cgatcccctc cgagagcagc gcgccgatcc ccaccgcgct
     5641 cttgacacca cccgccggca tcgggccggc ctcggtgacg ccgagatgca gcggatagtc
     5701 gcaggcctgc gcgagcaacc ggtacgcctg gatcatgacg ccggcgttcg agtgcttgac
     5761 cgagatcttg atgtcgaaga agtcctcctc ttcgaggatc ctggcctcgt acagcgcgct
     5821 ctccaccagc gcctccgccg tcggagcgcc gtgcctggcc agcacgtctt tctcgagcga
     5881 ccccgcgttc acgccgatgc ggatcggcac gccccgagcc ctggcctcgc gtgcgatcac
     5941 ccggaccttg tcctggaact tgatgttgcc cgggttgatg cgcagcccct ggatcccggc
     6001 ctcgagcgcc gccatcgcgt acttccactg gaaatggatg tcggcgacca ccgggacggt
     6061 cgacttggcc gcgatcgccg tcagggcctc ggcgtcctcc cagtgcggca cggccacccg
     6121 tacgatgtcg acgccggccg cgaccagcga ggcgatctgc tgcagggtcg cgttgatgtc
     6181 ggcggtcttg gtcgtggtca tcgactggac gcggatcggc gcgtcgccgc cgaccgtcac
     6241 gtcgcccacc cgcacctgcc gggtcttcct gcgttcggtc agcacggtcc cagtctccca
     6301 tccctcgggg ggctacccct gagccccccg gcccgcctac tcgccgaata cgtggaagac
     6361 cacctggcgg tccttgggtt cgtccatctc gaacaggatc aggcgctgcc accgccccag
     6421 catcgccgtg ccgcccgcca ccgggatcgc gtgggaggag gccgagagca gaatcgcctt
     6481 cacgtgggcg gggccgttcc ggcgctcgtg cgactcgtcc aggttctgcg tgcggcgcgt
     6541 ggcgtcgtca tgcgcgtggt acacgtcgtc ggggaccaga cgcaggatcc gggctcggaa
     6601 gtcctcgagc gcgccgtcct cccattcgtt gacgatcagc gcgcacgtcg tgtgaggaca
     6661 gaacgcaacc accgatccat cggtgacgcc cgagtccttg atcgcccgct cgagctcctc
     6721 cgtgagatcg aggaaggcgt agcgctcggg ggcggcgacc tcgatggacg tggtggcgga
     6781 tgtcattcgt tcctccttgt ctccttctgg cttgtctcct tctggcttgt ctccttctgg
     6841 cttgtctcct tccggcttgt ctccttctgg gggacggtgc tcgagcgctc cccaccgggg
     6901 tccccggacg gcgaggcgac cggtagctcg aacgtgaacg cgctcccgtg cccgggttcg
     6961 ctgtcggccc agatccgccc gccgtgcgcc tcgaccaccg aacgcgcgat ggcgagcccg
     7021 atcccggtcc cgccgtcgtc gcgcgaccgc gactcgtcca cccggtagaa gcgttcgaac
     7081 acgtggggca ggtgctcggc cgggatgcca gggcccgtgt cggcgacgac cacgtcgatc
     7141 gaaccgttgc ggttcgacgc ggtgaccgtc acgcgcccgc ctgtcggcgt gaaccgcacc
     7201 gcgttgtcga gcaggttgaa cagcacctga tggacacgct cgcggtctgc gaagaccggc
     7261 gggagggctc ccggcaggtc gtcgtcgagc gtcacgcccg ggtcggggcg ggcgaccagg
     7321 atctcggaga acacctgcga gaccagtggc gcgagctcga cgcgttcgcg ctccagcggc
     7381 acgtcgcccg actcgagccg cgataggtcg aggagctggt ccacgagccg tcccagccgc
     7441 tcggattgca ggagcatgat ctgcagcgtc tcggggtcgg gctgctcgac gccgtccagc
     7501 aggttctcca ggtgcgcccg cagcgccgag atcggggtct tgagctcatg cgacacgttc
     7561 gccacgagct cgcgccgcag gagctcgagc cccccgagtt cggtgctcat ccggttgaac
     7621 gcctcggcaa gcctgccgac ctcgtcgcgc gagtccgtgt ggatccgctg cgagtagtcc
     7681 ccggtctcca tccgacgggc cgctcgcgcc atgtcgcgga gcggctgcgt catccctcgt
     7741 gcgacccatc gagcgatcac gagcgcgatg ccggcgccga ggactccggc gagcaggagc
     7801 tccaaccaat gctcgccgat cgtgaagacc tcgaacccca cgccgaggat cagatagacg
     7861 agcaggaggg tcatcccgac ggcgaacacg atgaccgatc cgagcttcga acggatcgtc
     7921 ggcagccgct gcaggagcgt cacggcccgt tcacgcgtct ccgaacgcgt agccgacgcc
     7981 gtgaaccgtg cggacgaacc tcggctcggc gccatcctcc cccagcttcc ggcggatgcg
     8041 cgccacgtgc gaatcgacga cccgtccacc cgcgtagtcg cggtagcccc agacgcgatc
     8101 catcagctgc tcgcggctga ggacgacgcc gggctcgcga gcgagcgcga gcaggatctc
     8161 gaactcgagc ggggtgagct gcacctcctc cccgtccacg ttcaccttgc gtttggcggg
     8221 gtcgaccgag agcccgccgt ggtcgatcgg ctcggtcgcg ggcggcgagg tgatgcgttc
     8281 gacgcgtcgc aggatcgccc tgacccggac cgtcagctct cggaggctga acggcttggt
     8341 gaggtagtcg tccgcgccca ccgcgaaccc ggccaccttg tcggcctctt cggtccgcgc
     8401 cgtcagcatg agaacgggca cccagcgccc ggcctggagc ctcctgcaca cctcgagacc
     8461 gtccatcccg gggagcatca ggtcgagcac caccaggtcg ggttcctcgg cgctcgccag
     8521 ctccagcgcc tgcgggccgt cggccgcgac cagcacccgg aacccctcgc tcgagagccg
     8581 ggcgcggacc gcttcggcga tcgcggtctc gtcgtccacc accaggacgg tctgctcgac
     8641 caccggccca tcgtagcggc cgggcctgcg gggccctcgg ggacggtgtt gagaaatcgt
     8701 ggcgccggtc gcgtgcagcc gatggcgtag cacgaacgtc cggcgcggag gaacggccta
     8761 gacgctgggc ggcttcacga ggtccacgta gaccacggcg accgtgaaga cgatgaagaa
     8821 cgccgcgacc gcggccgaga cggggatgac cttgcgcatg tcgaccgggc ggccccgaat
     8881 cttctcgatc gcgaggaccg cgaggtgtcc cccgtcgaac ggcggcagcg gaagcaggtt
     8941 cagcagaccg acgaacacgt tcacgacgcc gaacaggagt aacaggtccc cgaaccgatc
     9001 gtcggaggcg aggctgccgg cgactcggcc gattccgacg acgctggcgg ggtcctcaac
     9061 ccttcgaggt tcgtcggtga atatgagggt ggcgaggcgc ccgatcccct cgggtccgaa
     9121 gatccgtgca accccatgca ccgtgcccac gatgttgtcg ccgacgagat cggcggcgcc
     9181 ggtgaccgac ccgattacgc cctgggtgcc gcggaccacc gttcgtggtt ggacgcccac
     9241 gccgacgcgg ccgatcacct cgccgccgac ctgcgagagc gccggcacca tcacggcccg
     9301 gaaggtctcg ccgtctcgcc ggatcgtgaa cgtcacgggc tcgccggcgt gcgcggtggt
     9361 gtacgccacc agctcgtcgg cggagggatc ggccaggtcg tccacgacga ccaccacgtc
     9421 gccggaccga agtccggccg ccgcggccgg cgactcgacg ccgtgcaggg tcggcttgac
     9481 cgcggagatc acgacgccgc gcgcgatcgg ctggcccacg agccccagcc acagcgcgaa
     9541 gcacaggaac gccaggacga aatgggtcgc cggccccgcg aggatcacga gcgcacgttg
     9601 ccagatcggc ttggccccga acgtccgtgg gaggtccgcg ggcgcgaccg tctcgtacgg
     9661 gttcatcccc gcgatcttca cgtagccgcc ggcggggatc gccttgaggc ccacctcggt
     9721 ctcgccactc atccgggacc agagcttcgg accgaagccg acgaagaact cgctcacctt
     9781 gatcccgaac gcccgcgcga ccgcgaagtg cgcggcctcg tggatcacga tcacgaggat
     9841 caaggcgacg atgaaggcac cgatgccgag cgccgccacg gtcgaccttc ctaccgagcc
     9901 tcgatgatct cggcggcacg ccggcgagcc caggtatcgg cccgctcgag gttcaccacc
     9961 gagaccacgc tcgagggcgc ctcgtgctcg tcgacgacgg ccgtgacgac ctcgacgatg
    10021 cgcgtgaggg ggatc
//
LOCUS       DATBRK010000321         5269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2799368, whole genome shotgun sequence.
ACCESSION   DATBRK010000321 DATBRK010000000
VERSION     DATBRK010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2799368"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VIH70_09555"
     CDS             <1..806
                     /locus_tag="VIH70_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY8116647.1"
                     /translation="DPDGWDDSVDADRRKALRLIAKTPTLLAMYHRMRTGQEIVPPNT
                     KLPHAANFLHMLLGLEPSQEDAEVIDTTFVLYADHTMNASTFTARIVASTLSDMFSAI
                     TAAIAALKGPLHGGANEESMKMLEEVGSPDRAEAYVRDRLERHQKVMGFGHAVYKTMD
                     PRAVVLKRLSKETGERHGDTSWYEISEAIERTVGEQKGLFPNVDFYAASVYHALGIPT
                     DLMTPIFAVARMAGWTAHVREQYADNRVIRPGSEYIGPRDQSWVPIEER"
     gene            complement(814..1242)
                     /locus_tag="VIH70_09560"
     CDS             complement(814..1242)
                     /locus_tag="VIH70_09560"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HEY8116648.1"
                     /translation="MAETVTIQGQAYLKRNPLGVLGLTIITLGIYGLYWYYKVNEEIL
                     RYTGDQTISPSRSLLAVIPGFLLIVPPFIAYYNTSNHVVQMQQQRGIQSQISPALVVI
                     LALVIWIGMAAYVQEHLNRVWDAAATGGAQPAAPPPPVPA"
     gene            complement(1274..2200)
                     /locus_tag="VIH70_09565"
     CDS             complement(1274..2200)
                     /locus_tag="VIH70_09565"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY8116649.1"
                     /translation="MSEPASRLESLARSRRFCVTSEVVPPRSSDPEPVERQARELVGY
                     ADAVNVTDNPTASSHMSPVAGTAIVARTGLEPTLQLTVRDRNRLAIAADLLGAWGLGA
                     RNLLCLTGDPLSVGDEPDAATVNDLGVLDLIALASTLREDSKLPSGTEIAPAPRYFVG
                     VADSPLAEHYDPARLEVKLDAGAGFVMTQIAYDLDRLAAWAEVVRARGVFERTAVFVG
                     VAPLRSAKQARYMNEHLPGVHVPDALLRALEDAGPWAETLGTAQCVEIVSGLRAIPGI
                     SGVHVMGLGREESVRHVIEGAGLLPRPAPGPA"
     gene            complement(2197..3063)
                     /locus_tag="VIH70_09570"
     CDS             complement(2197..3063)
                     /locus_tag="VIH70_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEY8116650.1"
                     /translation="MTATILDGRAVAAQIKAQVAERAMALVDRGIQPGLAAVLVGADE
                     ASRIYVGAKHKACSEVGFRSEQVDLPGFITEGELLATIRRLNRDPEIHGIIVQLPLPK
                     HISELAVQTTIDPRKDVDGLHPLNVGLMVRGEPSFPPATPYGIVELLLRSGVELEGAE
                     IVVAGYGDLVGAPLSILLAQDWIRGNATVTVCHVKTRDLAEHTRRADILVAATGRPQL
                     VTADMIKPGAVVIDVGVHRTEHGLVGDVAFDEVKEVAGMITPVPGGVGPMTTAMLLLN
                     TVSAAEKLSEQR"
     gene            complement(3130..3747)
                     /locus_tag="VIH70_09575"
     CDS             complement(3130..3747)
                     /locus_tag="VIH70_09575"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8116651.1"
                     /translation="MPDDPREIVEAGYDAIADRYAEAIRAGRGLETYFRSFLARVLEL
                     IPEGSTVLDLGCGAGLVTADLTTRASVVGVDISAGQLELARRNVPAARFVRADMVDLA
                     FAPGSFDAVVAFWTLIHVPREMHASLFTRIHGWLKPGGLFAGTLGSGDNPAEHVPDFY
                     GAPMYWSHFDGDTNRGLIRDAGFDLVHADEIEDEGETPLWVIATA"
     gene            complement(3752..>5269)
                     /gene="purH"
                     /locus_tag="VIH70_09580"
     CDS             complement(3752..>5269)
                     /gene="purH"
                     /locus_tag="VIH70_09580"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016342000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY8116652.1"
                     /translation="ALLAVFDKEGVVELARGLSAHGVELVSSGGTAATIAAAGIDVTP
                     VEAVTGFPEMLDGRVKTLHPKIHAGILADRRKPEHRSQLADHGIEPIDLVIVNLYPFR
                     ETVASGAGEDDVIERIDIGGPAMVRAAAKNFVSVGVVVSPSRYGEVLDAIGTQGGLSL
                     ETRRELAAEAFAHTAAYDAAVAGWFASDDAAMPAFVGLAFDKVTDLRYGENPHQRGAL
                     YRGAGGPGVLGGAEVLVEGKEMSFNNWLDVDAALTLAADLEPLGPAAVIVKHNNPCGA
                     AVGDTLAASYASAFESDTVSAFGGIVAFGREADEATTRAMAEVFTEVVVAPSFTGPAL
                     AAFAERKGLRVVRAPLPEPGDLDVRVLPGGALVQDADAAPESAADLEVVSTRRPTEDE
                     WRDLRFAWVVSMRVKSNAIVFARELATVGVGAGQMSRVDASVLAVRKAGDRAKGAVMA
                     SDAFFPFPDAVEVAADAGVTAVIHPGGSIRDEEVLAMAEARGMAVVVTGRRHFRH"
BASE COUNT          824 a         1922 c         1777 g          746 t
ORIGIN      
        1 acgaccccga cgggtgggat gactcggtgg acgcggatcg gcgcaaggcg ctccgcctga
       61 tcgcgaagac cccgaccctg ctcgcgatgt accaccggat gcggacgggt caggagatcg
      121 tcccgcccaa caccaagctc ccgcacgcgg cgaacttcct ccacatgctg ctcggcctcg
      181 agccgtccca ggaggacgcc gaggtgatcg acacgacgtt cgtgctgtac gccgatcaca
      241 cgatgaacgc ctccaccttc accgctcgga tcgtcgcctc gacgctatcc gacatgttct
      301 cggcgatcac cgcagcgatc gccgcgctca agggaccgct gcacggcggc gccaacgagg
      361 aatcgatgaa gatgctcgag gaggtcgggt cgccggaccg cgccgaggcg tacgtgcgcg
      421 accggctcga gcgccaccag aaggtgatgg ggttcggaca cgccgtctac aagacgatgg
      481 acccgcgggc ggtggtcctc aagcgcctct cgaaggagac gggcgagcgg cacggggaca
      541 cgtcctggta cgagatctcg gaggcgatcg aacgcaccgt cggcgagcag aaaggcctgt
      601 tcccgaacgt cgacttctac gcggcgagcg tgtaccacgc gctcggcatc ccgaccgacc
      661 ttatgacgcc gatcttcgcg gtggcacgga tggccggatg gacggcccac gtgcgcgaac
      721 agtacgcgga caatcgcgtg atccggcccg gatcggagta catcggaccc cgggatcagt
      781 cctgggtccc gatcgaggaa cggtagggcc ggctcaggcc gggaccggag gcggcggagc
      841 cgcgggctgc gcgccacccg tcgcggccgc gtcccagacg cggttcaggt gctcctgcac
      901 gtaggccgcc atcccgatcc agatcacgag cgccaggatg acaacgagcg cgggcgagat
      961 ctgcgattgg atcccccgct gctgctgcat ctggacgacg tggttcgacg tgttgtagta
     1021 ggcgatgaac ggcgggacga tcagcaggaa gccggggatc acggccagga gcgagcgaga
     1081 cgggctgatc gtctggtcgc cggtgtagcg caggatctcc tcgttcacct tgtagtacca
     1141 gtacaggccg tagatgccca acgtgatgat cgtgagcccc agcacgccga gtgggttccg
     1201 cttcagatac gcctgtccct ggatcgtcac cgtctcggcc acgtcgcctc cccccgtcgg
     1261 ttgctcggcg agtctacgcc gggccggggg cgggccgtgg gagcagcccg gctccctcga
     1321 tcacgtggcg cacggactcc tcgcggccga gccccatcac gtgcacgccc gagatgcccg
     1381 gaatcgcccg cagccccgag acgatctcga cgcactgggc ggtcccgagg gtctccgccc
     1441 agggtcctgc atcttcgagc gcgcggagca gcgcgtccgg aacgtggaca ccgggcaggt
     1501 gctcgttcat gtagcgggcc tgcttcgcgc tccgaagcgg cgccacgccg acgaacacgg
     1561 ccgtccgctc gaacacgccg cgggcgcgga cgacctcggc ccacgccgcc agccggtcca
     1621 ggtcgtaggc gatctgggtc atcacgaacc cggcgccggc atcgagcttg acctcgagtc
     1681 gcgccgggtc gtagtgctct gcgagcgggc tgtccgccac gccgacgaag tagcggggcg
     1741 ccggggcgat ctcggtcccc gatgggagct tcgagtcctc acggagcgtc gaggcgagcg
     1801 cgatgaggtc gagcaccccg aggtcgttga ccgtagccgc gtcgggctcg tcaccgaccg
     1861 acagcgggtc gccggtgagg cacagcaggt tgcgggcgcc gagcccccag gcccccagga
     1921 gatcggccgc gatcgccagc cggttccgat cgcggaccgt gagctgcagg gtgggctcga
     1981 gtcccgtcct ggcgacgatc gcggtgcccg cgaccggcga catgtgcgag ctcgcggtcg
     2041 ggttgtcggt gacattgacg gcgtccgcgt agccgacgag ctcgcgggcc tggcgttcga
     2101 ccggttcggg atcgctcgac ctcgggggga cgacctcgga ggtcacgcag aagcggcgcg
     2161 agcgcgccag cgactcgagc cggctggccg gttcgctcaa cgttgctcgg agagcttctc
     2221 ggccgcggag acggtgttga gcagcagcat cgcggtggtc atcggaccga cgccgccggg
     2281 gaccggtgtg atcatccccg cgacctcctt cacctcgtcg aacgccacgt cgccgaccag
     2341 gccgtgctcg gtccggtgca ccccaacgtc gatcacgacc gcgcccggct tgatcatgtc
     2401 ggcggtcacg agctgcggtc gtcccgtcgc ggcgaccagg atgtcagcgc gccgagtgtg
     2461 ctccgccagg tcgcgcgtct tgacgtgaca gaccgtcacg gtcgcgttgc cacggatcca
     2521 gtcctgcgcg agcaggatcg acagcggcgc cccgaccaga tcgccgtagc ccgcgaccac
     2581 gatctcggcg ccctcgagct cgaccccgga gcgcagcagg agctcgacga tcccgtatgg
     2641 ggtcgccggg gggaacgacg gctcgccccg gaccatcagc ccgacgttga gcggatgcag
     2701 gccgtccacg tccttgcgcg gatcgatcgt ggtctgcacc gcgagctcac tgatgtgttt
     2761 cggcaacggg agctgcacga tgatcccgtg gatctccggg tcgcggttca accggcggat
     2821 cgtcgcgagg agctcgccct ccgtgatgaa cccgggcagg tccacctgct ccgaacggaa
     2881 cccgacctcg gagcacgcct tgtgcttggc tcccacgtag atccgtgacg cctcgtcggc
     2941 gcccacgagc acggccgcga ggcccggctg gatcccgcgg tccacgagcg ccatcgcacg
     3001 ttcggccacc tgcgccttga tctgcgcggc gaccgccctg ccgtccagga tcgtcgcggt
     3061 cacgagccga atcctaccga cgggccgggt gcccagcccg caggagccga ccgacccgcg
     3121 gaccgacggt caggccgtcg cgatcaccca gagaggggtc tctccctcgt cctcgatctc
     3181 gtcggcatgg acgaggtcga agccggcatc ccggatgaga ccgcggttcg tgtcaccgtc
     3241 gaagtggctc cagtacatcg gcgccccgta gaagtccggg acgtgttccg ccgggttgtc
     3301 gccgctcccg agcgtccccg cgaacaggcc gcccggcttc aaccagccat ggatgcgcgt
     3361 gaacagggac gcatgcatct cacgcggcac gtggatcagc gtccagaaag ccaccaccgc
     3421 gtcgaacgat cccggcgcga acgcgagatc caccatgtcc gcgcgcacga acctggcggc
     3481 cggcacgttc cggcgcgcga gctcgagctg ccccgccgag atgtcgaccc ccacgacgct
     3541 ggcacgggtc gtgaggtcgg cggtgacgag gccggcgcca cagccgaggt ccaggaccgt
     3601 acttccctcg gggatcagct cgaggacgcg ggcgaggaag ctccggaagt acgtctcgag
     3661 gcctcgaccg gcgcggatcg cctccgcgta ccgatctgcg atcgcgtcgt accccgcctc
     3721 gacgatctcg cggggatcgt cgggcatgcg ctcagtgccg gaagtgccgc cgcccggtca
     3781 cgacgacggc catgccccga gcctctgcca tcgcgagcac ttcttcgtcc cggatcgaac
     3841 ccccggggtg gatcacggcc gtcaccccgg cgtccgccgc gacctcgacc gcgtcgggga
     3901 acgggaagaa cgcgtcgctc gccatgactg cgcccttcgc gcggtcgccc gccttccgca
     3961 cggccagcac cgacgcgtcc acccgcgaca tctggccggc acccacgccg accgtcgcga
     4021 gctcccgcgc gaacacgatc gcgttcgact tcacacgcat cgagacgacc cacgcgaacc
     4081 gaaggtcgcg ccattcgtcc tcggtgggtc gccgcgtcga cacgacctca agatcggccg
     4141 cgctctcggg tgcggcgtcg gcgtcctgca cgagcgcgcc cccgggaagc acgcgcacgt
     4201 cgagatcccc cggttccggc aatggcgctc ggaccacgcg caggcccttc cgttcggcga
     4261 acgccgccag cgcgggcccg gtgaaggacg gcgcgaccac gacctcggtg aacacctcgg
     4321 ccatcgcccg cgtcgtggcc tcgtccgcct cacgcccgaa cgccacgatc ccgccgaacg
     4381 cgctcacggt gtcggactcg aacgccgacg cgtacgacgc ggcgagcgtg tccccgaccg
     4441 ccgcgccgca cggattgttg tgcttcacga tcacggccgc gggaccgagc ggctcgaggt
     4501 ctgccgcgag cgtgagcgcc gcgtccacgt ccaaccagtt gttgaacgac atctccttgc
     4561 cctcgacgag cacctccgcg ccaccgagca ccccgggccc acccgcccct cgatacaacg
     4621 ccccgcgctg atgcgggttc tcgccatagc ggaggtccgt caccttgtcg aacgcgagcc
     4681 cgacgaacgc cggcatcgcg gcatcgtccg aggcgaacca tccagcgacc gccgcgtcgt
     4741 aggccgccgt gtgggcgaac gcctcggcgg ccagctcccg ccgcgtctcg agcgacagac
     4801 ctccctgggt cccgatcgcg tcgaggacct cgccgtagcg cgacgggctc acgacgacgc
     4861 cgaccgagac gaagttcttc gcggccgcgc ggaccatcgc gggaccgccg atgtcgatcc
     4921 tctcgatcac gtcgtcctcg cccgcaccgc tcgccacggt ctcgcggaac gggtacaggt
     4981 tcacgatgac gagatcgata ggctcgatgc catggtcggc gagctgtgac ctgtgctcgg
     5041 gcttgcgccg gtcggccagg atgcccgcgt ggatcttggg gtgcagggtc ttcacgcgac
     5101 cgtcgagcat ctcggggaac ccggtcaccg cctccaccgg cgtcacgtcg ataccggccg
     5161 ccgcgatcgt cgccgccgtc cccccggagg agacgagttc gacgccgtgc gcggagagcc
     5221 cgcgtgcgag ctcgaccacc ccttccttgt cgaacaccgc gaggagcgc
//
LOCUS       DATBRK010000322         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2079132, whole genome shotgun sequence.
ACCESSION   DATBRK010000322 DATBRK010000000
VERSION     DATBRK010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079132"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VIH70_09585"
     CDS             complement(<1..356)
                     /locus_tag="VIH70_09585"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEY8116653.1"
                     /translation="MSDTVYALYACLKATRAFRDLDPDDAAQEVENLIKEWDPAVTVR
                     GVYSTVGFRADTDLMLWLVGPTPDAVQDALVAFRRTRAGRELDMTWTFMGVVRPAEFT
                     PDHAPAFVKGEPPRRYL"
     gene            complement(353..1792)
                     /gene="hemG"
                     /locus_tag="VIH70_09590"
     CDS             complement(353..1792)
                     /gene="hemG"
                     /locus_tag="VIH70_09590"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEY8116654.1"
                     /translation="MVVPTAARVVVVGGGVSGLTAAYRLLEADPALELAVLESEPDAG
                     GRLRTATVGDLELEAGPDSFVARKPWAVDLCRELGLELTAPGARDALIWTERGLVPLP
                     ESALGIPTDVEEIARWPGLSRRGRVRALVDLVRKARAPKTDESIGSLVRRRMGDEVAE
                     RLTGPLLGGLFAGDVDRLGVDATFPELARWERAFGSLIRGARAASRAAADAGPMFLRP
                     RGGVGELPRALLGRVGRARVRLGAPVRSVERDGETFVVRTAREALRAEAVVVATPAFV
                     AAELLHGIDARAAGLLGSIPYASTGVVHLVYPEDTAGALPEATGFVVPLGRAPMTAAT
                     FVSRKWPEAAFGTRAVVRCFVGAVGSEDVLDEPDEDIVDAVCRHLAAFLPLPGRPTAS
                     SVVRWPRSMPQYEVGHLERVRAIEGSLPAGIFLTGNAYQGVGVADAVRSAGLAAERVR
                     VHLGAEPAGVDLEDGRGNERASSRSENVG"
     gene            complement(1795..>2016)
                     /locus_tag="VIH70_09595"
     CDS             complement(1795..>2016)
                     /locus_tag="VIH70_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY8116655.1"
                     /translation="ADLASQGCPAAVVCSAGFVADHLEILYDLDIEARAIAERAGMHL
                     VRTEMPNADPAFVRALADVVRERLAASRT"
BASE COUNT          285 a          787 c          719 g          225 t
ORIGIN      
        1 aggtagcgcc gcggcggctc gcccttgacg aacgcgggcg cgtggtcggg ggtgaactcg
       61 gccggcctga ccacgcccat gaacgtccag gtcatgtcga gctcgcgccc ggcccgcgtc
      121 cgccggaacg cgacgagcgc atcctgcacc gcgtcggggg tcggcccgac gagccagagc
      181 atcagatcgg tgtcggcccg gaagccgacc gtggagtaga cgccccgaac ggtcaccgcg
      241 gggtcccact ccttgatcag gttctcgacc tcctgggccg cgtcgtccgg gtcgaggtct
      301 cggaaggcgc gcgtcgcctt caagcaggcg tacagcgcgt agacggtgtc gctcatccca
      361 cgttctccga tctgctcgaa gctcgttcgt tgccgcgccc gtcctcgaga tcgacgcccg
      421 cgggctcggc cccgagatgg acacggaccc gctcggcggc gagccccgcg ctacggacgg
      481 cgtcggcgac gccgaccccc tggtacgcgt tgccggtcag gaagataccc gccggcagcg
      541 agccctcgat cgcgcggacg cgctcgaggt gcccgacctc gtactggggc atcgaccgtg
      601 gccaccggac caccgacgat gcggtcgggc gtccgggcag cgggaggaag gccgccaggt
      661 gccggcacac ggcgtccacg atgtcctcgt cgggttcgtc caggacgtcc tccgaaccca
      721 cggccccgac gaaacagcgg acgacggcgc gcgtcccgaa cgccgcctcg ggccacttcc
      781 gcgagacgaa cgtcgccgcc gtcatcggcg cccggcccag gggcacgacg aacccggtcg
      841 cctcggggag ggccccggcc gtgtcctccg ggtagacgag gtgcacgacg ccggtgctcg
      901 cgtacgggat cgagccgagc aacccggccg ccctcgcgtc gatcccgtga agcagctcgg
      961 ccgcgacgaa cgccggcgtg gcgacgacga ccgcctcggc gcgcagggct tcccgggcgg
     1021 tgcgcaccac gaaggtctcg ccgtcgcgtt cgaccgagcg gaccggcgcc ccgagccgga
     1081 cgcgagccct gcccacgcgc ccgagcagcg cgcgcgggag ctccccgacg ccgccgcgcg
     1141 gtcgcaggaa catcggcccc gcgtcggcgg cggccctcga cgccgcgcgg gccccgcgga
     1201 tcaggctgcc gaacgctcgc tcccaccgcg cgagctcggg gaacgtcgcg tcgacgccca
     1261 gccggtcgac gtcgccggcg aacaaccccc cgaggagcgg cccggtcagt cgctcggcca
     1321 cctcgtcgcc catccgccgg cgtaccagcg agccgatcga ctcgtcggtc ttcggcgccc
     1381 gagccttgcg cacgagatcg acgagcgctc gcacgcgacc ccggcgcgac aggcccggcc
     1441 agcgcgcgat ctcttccacg tcggtgggga tgccgagcgc gctctccgga agcgggacga
     1501 gcccgcgctc ggtccagatg agggcgtcgc gggcgcccgg cgcggtcagc tcgagaccaa
     1561 gctcccggca caggtccacc gcccacggct tgcgcgcgac gaacgagtcc ggaccggcct
     1621 cgagctcgag gtcgccgacg gtcgccgtcc gaagccggcc gccggcgtcg ggctcggact
     1681 ccagcacggc gagctcgagc gcgggatcgg cctcgagcag ccggtacgcc gccgtcaggc
     1741 ccgagacccc gccgccgacg accacgaccc gcgcggcggt cggcaccacc accgttaggt
     1801 cctcgaggcc gcgagccgct cgcggacgac gtcggcgagc gcacggacga acgcgggatc
     1861 cgcgttcggc atctcggtgc gcacgagatg catgccggcc cgctcggcga tcgcccgcgc
     1921 ctcgatgtcg aggtcgtaca ggatctcgag gtggtcggcc acgaacccgg ccgagcacac
     1981 gacggcggcc ggacacccct gcgacgcgag gtccgc
//
LOCUS       DATBRK010000323         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1931819, whole genome shotgun sequence.
ACCESSION   DATBRK010000323 DATBRK010000000
VERSION     DATBRK010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931819"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VIH70_09600"
     CDS             complement(<1..309)
                     /locus_tag="VIH70_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116656.1"
                     /translation="MLGLQLPRSRSAHRRSRPYRYVVSLLATLAVLVVPTVASATNGF
                     TEEVVSARWLISFQCPDGTTAADGRLIVETDNFIEAGTTRDPNPPLRVGFVGQCPDGT
                     F"
     gene            902..1609
                     /gene="pcm"
                     /locus_tag="VIH70_09605"
     CDS             902..1609
                     /gene="pcm"
                     /locus_tag="VIH70_09605"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY8116657.1"
                     /translation="MNDGWLIPPPSQPVKSDEDLRREREAKIGELERRGLLRSERLRR
                     AMLTVRREDFIPSRYRDHAYEEIPLPLPGERATISCPHSYPLFYEPLDLGEGHRFLEV
                     GVGSGYGTSLAREVVGPEGLVVAIDIDATTLDFARENLERAGYADVVLIHGDGGLGYP
                     EHAPYDRICVTAACPEVPPPLIEQLAVRGRLIVPVMEGTRQRLTLLEKTVDGVRRKSL
                     ADVLYVSLQGRFGVRSD"
BASE COUNT          275 a          543 c          569 g          250 t
ORIGIN      
        1 gaaggtgccg tccggacact ggcccacgaa gccgaccctg aggggcggat tggggtcacg
       61 cgtcgtcccc gcctcgatga agttgtccgt ctccacgatc aggcggccgt cggcggccgt
      121 ggtgccatcc gggcactgga acgagatcaa ccagcgggcg gagacaactt cctcagtgaa
      181 gccgttcgtg gccgatgcga cggtcggtac gacaagcacc gccagggtgg cgagcaggga
      241 gacgacgtac ctgtacggcc gtgaacgtcg gtgggcggat cggcttcgcg gcaactgcag
      301 accaagcatg agcgacctcc ctgtgggtgg agccggttaa cgagaccgtc ggtcggtcgt
      361 acgccgtgga gtcgagcgag accggcagac gagaaacacc acaccagggg ttacgcgcgg
      421 cgaccggcat cggatcgagc gcatcgagca ccccgccgaa cctccgtcgc gcacaacctc
      481 gcgatcgggc tccgggtgtc tcgctggcat cgcccacgcc cagaccatca acacggttcc
      541 cggcggctac gcccgccgcc caacgcctcg ctccacgctc tagaggccga tctggcggct
      601 gcttccgcgt ctcagcacgg catatgtgct ggtgggcttg cgctgcgcgc gtcaaagcag
      661 gcgctcggcg gcggccagac gacgatcgct ccggacgagg agcaccggca tcccggactt
      721 ccacgcgccg ctcctccgcg cataggagca gtggctgagc cttcgggcgc cgcgatcgtc
      781 gataccgtgg ccgcgtgacg tctgatgaaa cgctcgaggc gatgcacgag gtgttcggtc
      841 ggcgaacagt cgagtctgag taacgggcgg tgcaccggga cgcctcgggt cacttaggcc
      901 gatgaacgac ggatggctca ttccgccgcc gagccaacca gtcaagagcg acgaggatct
      961 gcggcgggag cgggaagcga agatcgggga gctcgagcgg cgcgggctcc ttcgctccga
     1021 gcggctccgc agggcgatgc tcaccgtgcg tcgtgaggac ttcatcccgt ctcgctaccg
     1081 ggaccacgcc tacgaagaga tccccctccc tctcccaggc gagcgcgcca cgatctcgtg
     1141 cccgcatagc tatccgctgt tctacgagcc gctcgacctg ggggaggggc accggttcct
     1201 tgaggtcggc gttgggtcgg ggtacggcac gtccttggcg cgggaggtgg tgggtccgga
     1261 aggtctggtc gtggccatcg acatcgacgc cacaacgctc gacttcgccc gggagaacct
     1321 ggaacgggca ggctacgccg atgtggtcct gattcacggc gacggaggac tcgggtaccc
     1381 cgagcacgcg ccctacgacc ggatctgcgt cacggcggcc tgccccgaag tcccgccgcc
     1441 gctgatagag caactcgcgg tgcgcggccg gctgatcgtg cccgtcatgg aaggcacgcg
     1501 ccagcgactg acccttctcg agaagaccgt cgacggcgtc cggcgcaaga gcctcgcgga
     1561 cgtgttgtac gtgtcgctgc agggacggtt cggcgttcgt agcgattgaa ctcccgttgc
     1621 gagaccgagt gtccggc
//
LOCUS       DATBRK010000324         8425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_492898, whole genome shotgun sequence.
ACCESSION   DATBRK010000324 DATBRK010000000
VERSION     DATBRK010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492898"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VIH70_09610"
     CDS             complement(<1..490)
                     /locus_tag="VIH70_09610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEY8116658.1"
                     /translation="MADAGVREGDRVVEVGAGLGSLTVPLAEAGARVLAIEVDHGLSR
                     ALREVVGSYPNVRIEVTDAMRTDWTTALGADHWSMVSNLPYNVSVPLLMELLRTAPID
                     RYVVMVQREVGERLLARPGEEAYGAVSVRVAYGADARVLRRVPADVFWPRPKVDSVLV
                     RLD"
     gene            complement(609..1712)
                     /locus_tag="VIH70_09615"
     CDS             complement(609..1712)
                     /locus_tag="VIH70_09615"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HEY8116659.1"
                     /translation="MPVRPSRVRRLRVRKAMGNALLASVVLVGGLTYVAIQKTVTLVV
                     DQGRPEAVRTMSASVGELLDAQGILVGNGDLVTPPTQTSLADGMTVIVERGWLDGITG
                     PGPDDVGVWVMEGVTASHLMLASRSGEDWFSAGDPAGWSQRVSADVVVKGKDHDVLTN
                     ADTVRELLSAMGITPDRDDRVLPSPRTPLQEGMRVRYDAIAYRTRDVRVPIPYTTSTS
                     YSDTESPGAVSLERAGVDGVMVERTRVTFVNGEAVATEVLSRTVLQEAIAARRVIGRE
                     KQTDGEHETDGTQVGEASWYSFAPGAGLTAAHPWLPFGTVVTVTNIANGETVQVVIND
                     RGPFGGRIIDLSEEAFARIAPLAQGVCQVRLSW"
     gene            complement(1826..2644)
                     /locus_tag="VIH70_09620"
     CDS             complement(1826..2644)
                     /locus_tag="VIH70_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY8116660.1"
                     /translation="MNESNPEPPMQSGAVDTHCHLFLLDDDPAEAIETARIRGVDTLV
                     CVGIDPGSSRRSVELAASFPGVVATAGMHPHDASDFDARASSEIEELLANPLVVAVGE
                     CGLDFFRMRSPRPDQERALRSQVAMAKAHDLPLVIHVRDAWPEVLRVLDEGSAESVIL
                     HCFSGDAEIAARCAERGWFLSFAGNVTYPKNAHLREAASAVALGRILVETDSPFLAPQ
                     RLRGRDNSPSNVLDVIEAVGSARDEPVEIVRETTAENAKIAFSRLREALEIPRD"
     gene            complement(2788..4326)
                     /gene="metG"
                     /locus_tag="VIH70_09625"
     CDS             complement(2788..4326)
                     /gene="metG"
                     /locus_tag="VIH70_09625"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY8116661.1"
                     /translation="MGKDVFYITTPIYYPNADPHIGHGYTSVAADFIARYHRLRGEEV
                     LLLTGTDEHGLNLQRRAEAAGKDPQTWVDEMAPRWREVWAGLDIGYDDYIRTTEPRHE
                     KAVQRLLSAVHDNGRDDIYLGIYEGLYCVSCEAYYTEDELVDGMCPIHGIPVEHVQEE
                     NYFFRLSAYADRLLEHYERRPEAVQPETRRNEVLSLIGGGLQDFSISRTNFRWGIPLP
                     WDPKHVCYVWFDALTNYVTAAGYGTDEDRFSRIWPANVHLIGKDILRFHAVYWPAMLM
                     AGGVEPPEQVWAHGFLTVGGQKMSKTNATGIHPFELTDHFGVDSYRYFFMREIQFGQD
                     GSFSWESMVDRHNADLANGLGNLASRILAMLAASFDGLVPEPEAGGVEDDLPALTADV
                     ARRYDEHMTALALTQALAAVWEIVGRANGYLVERQPWTLANDEVRRAELAGILYASAE
                     TLRILAILIAPIMPGAAARLWEQLGIEEPLEAQRLPGASAWGGLRPGTKTVKGDSLFP
                     RLDS"
     gene            complement(4369..5241)
                     /locus_tag="VIH70_09630"
     CDS             complement(4369..5241)
                     /locus_tag="VIH70_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116662.1"
                     /translation="MDRMNRPELPPPPALHPPDLPLPPAPWAAPARPAVPVTRRPART
                     LASVAVGLVLVLIAGIGPTLASHGSKVDPRPLFGGARSHAFIGRSDDGEPYRWDPCST
                     IHYQVDLGRLVDDHVLDDVKEAVRRVSRASGLAFEFDGVVHVSVGDLIGSGDFVTTTT
                     GGLRWSPVLIAFRSRSAMRALDAAGAYGVAIPVTTRFDAEQFVSAAVVINASAGLEDG
                     FDLAPSVGTVLQHELGHVVGLGHILDPFQLMSPVPVVTDWGTGDLAGLEELGEGPCLD
                     VPTAEYQAAIFPGP"
     gene            complement(5319..6152)
                     /gene="rsmI"
                     /locus_tag="VIH70_09635"
     CDS             complement(5319..6152)
                     /gene="rsmI"
                     /locus_tag="VIH70_09635"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY8116663.1"
                     /translation="MAGCLYVVGTPIGNLADLSDRARETLGMVDLVAAEDTRRTGRLL
                     ERIGVKARMLSLFEGNERQRIDELLDRLRNGERIALVSDAGMPAVSDPGFRFLRAAAD
                     AGIQIRVVPGPSAVTAALVVSGLPTDRWVFEGFLSHRPSERRERLRALAHDPRTVVLF
                     ESPLRVITLLRDALVELGDRRAVVARELTKLHEEVVRGRISEVIASLLEAEPRGEMVV
                     VIEGTEARTPGLDEMVEEARRLIADGMRKRDAAVAVARRGGGSANEIYGALIGSEIET
                     D"
     gene            6234..6686
                     /locus_tag="VIH70_09640"
     CDS             6234..6686
                     /locus_tag="VIH70_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEY8116664.1"
                     /translation="MAPSRSSAGHRDYSATPLARKLGIREGSRVLVVGAPGGFSLGPV
                     PGGVSFVRSARGPLDVVLLFTTTLADLRRRFPAAVRALDPAGRLWVAWPKKAAEVDTD
                     LTFEIVQRMGLDAELVDNKSASVDDVYQGLQFVIRLKDRAKRTAGRRP"
     gene            6715..8229
                     /locus_tag="VIH70_09645"
     CDS             6715..8229
                     /locus_tag="VIH70_09645"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY8116665.1"
                     /translation="MSTLTRPAPPFAQRPASASLRMHAWVRRFNRPVVAVLAVGLIAG
                     YLRFVHLAYPEHRVFDEYYYTKSACILLGYSDQRCDINSADERFWRENEWDTGAWVHP
                     PLGKWMIAFGELAFGTESMGWRAAAAVTGTATVMLLAVIIQLLWASPIWTFTGGLLLA
                     TESLNLVQSRTGTLDIFVAFWVVLVFVFLLLDRRWIDRRTPPPVPATDGPSDAGARVQ
                     PRRRLPAPLWRPYRFAAGAAGGAAMATKWSGLTAFVTAIALGFLWEVMRRKQFGMSKP
                     IVTTIMTEGFGFVLALLVTPAIVYVVTYIPWFLHFGWDLQRWVKMQGDAARFHADLKA
                     VNDQGQPWHAYFAPAWKWILLARPTYYYGAFGSDGVRQVIYAQGNPAIFWGSLLAIPY
                     VAYAWWRTRDWRAGFVIVTIAGLYLPWFLVSRPQFFFYATPLTPFFVLACVYALRDLS
                     ELHVAGSRSRPFLPFVVGFVAASVILFIWFWPILTAAPVSDAAFKLRYWFTSWA"
     gene            complement(8241..>8425)
                     /locus_tag="VIH70_09650"
     CDS             complement(8241..>8425)
                     /locus_tag="VIH70_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116666.1"
                     /translation="SSREGELLERWDARAGSVTLMRAPGLAGVRDGRPYHAVHGPPRG
                     ARCSLALRMRVDPPGS"
BASE COUNT         1260 a         2982 c         2894 g         1289 t
ORIGIN      
        1 ggtcgagccg gacgagcacc gagtcgacct tcggacgcgg ccagaacacg tccgccggca
       61 cccggcggag cacgcgggcg tcggccccgt acgcgacccg gacgctcacc gcgccgtagg
      121 cctcctcgcc ggggcgcgcg aggagccgct ccccgacctc gcgctgcacc atcacgacgt
      181 accggtcgat cggggccgtc cgcaggagct ccatcaggag cgggaccgag acgttgtagg
      241 gcaggttcga gaccatcgac cagtggtcgg cgccgagcgc cgtcgtccag tcggtacgca
      301 tcgcgtcggt cacctcgatg cggacgttcg ggtaggaccc gacgacctcc cggagcgccc
      361 gcgacaaccc gtggtccacc tcgatcgcca ggacccgcgc tcccgcctcg gcgagcggga
      421 ccgtcagcga cccgagaccc gcgccgacct cgacgacccg atcgccttcc cgaacgccgg
      481 catcggccac gatcgcgcgg gcgaggttcg ggtcggcgag gaagtgctgg ccccggatcc
      541 tggtgggccg gatcccgtgg cgcgccgcga ggtccttcag ttgggagcgg ccgaggccgt
      601 ccccgcgctc accagctgag ccggacctga cagacgcctt gggcgagcgg ggcgatccgg
      661 gcgaacgcct cctcggagag gtcgatgatc cgtccgccga agggtccccg atcgttgatc
      721 accacctgaa cggtctcgcc gttcgcgatg ttcgtgacgg tgaccaccgt accgaacggg
      781 agccacgggt gggccgcggt gagcccggcc cccggcgcga acgagtacca gctggcctcg
      841 ccgacctggg tgccgtcggt ctcgtgctcg ccgtcggttt gcttctcgcg gccgatgacg
      901 cgccgggcgg cgatcgcttc ctgcaggacg gtcctggaga gcacctcggt ggcgacggcc
      961 tccccgttca cgaacgtgac ccgcgtgcgc tccaccatca cgccgtcgac ccccgcgcgc
     1021 tcgaggctga ccgcgccggg gctctcggtg tcggagtagc tcgtggacgt ggtgtacggg
     1081 atcgggacgc ggacgtcgcg ggttcggtag gcgatcgcgt cgtaacggac gcgcatcccc
     1141 tcttggaggg gggtcctggg tgacggaagg acgcggtcgt cgcggtcggg tgtgatcccc
     1201 atggccgaga ggagctcgcg aacggtatcg gcgttggtga gaacgtcgtg gtccttcccc
     1261 ttcaccacga cgtccgccga caccctctgc gaccacccgg ccggatctcc cgcggagaac
     1321 cagtcctcgc cggaccgcga cgccagcatc agatgcgacg cggtcactcc ctccatcacc
     1381 cagaccccca catcgtcggg cccgggaccc gtgatcccgt cgagccaacc ccgctccacg
     1441 atgacggtca tcccgtccgc gagcgaggtc tgcgtcggcg gcgtcaccag gtcgccgttc
     1501 ccaacgagga tcccctgcgc gtcgaggagc tccccgacgc tcgcgctcat cgttcgaacg
     1561 gcctcgggtc gcccctggtc taccacgagc gtcacggtct tctggatcgc gacgtacgtg
     1621 agccccccca cgagcaccac gctcgcgagc agcgcgttcc ccatcgcctt gcggacccgc
     1681 aaacggcgga cccgcgacgg acgaaccggc acccttcctc ctcgcggaag aaggccccgg
     1741 ggggaagcgg ccgtggccgg accgaagcca tttttgacga gctaccgcga ggaccgtatc
     1801 aaaccctcaa cccttactgc aaacttcaat ctcggggtat ttcgagggcc tcccgaagcc
     1861 tggaaaaggc gatcttggcg ttctcggcgg tggtttccct cacgatttcc acaggttcgt
     1921 cccgagcgga cccgaccgcc tcgatcacgt cgaggacgtt cgacggcgag ttgtcgcgtc
     1981 cgcggagacg ttggggcgcg aggaaggggc tgtcggtctc gacgaggatc cggccgagcg
     2041 ccaccgcgga ggcggcctcg cggaggtgag cgttcttcgg ataggtcacg ttccccgcga
     2101 acgagaggaa ccaaccgcgc tccgcgcacc gcgcggcgat ctcggcatcg ccgctaaagc
     2161 aatgcaggat cacgctttcg gcggaaccct catcgagcac tcgaaggacc tcgggccagg
     2221 cgtcccgaac gtggatcacg agtggaaggt cgtgcgcctt cgccattgcc acctgcgaac
     2281 gcagcgcgcg ctcttggtcc ggtcgagggg agcgcatgcg gaagaagtcc agaccgcact
     2341 cgccgaccgc gaccacgagc gggttcgcga gcagctcctc gatctccgac gacgcccggg
     2401 cgtcgaagtc gctcgcgtcg tgagggtgca tccccgcggt ggcgacgacg ccggggaacg
     2461 acgcggcgag ctcgaccgac cgacggctcg acccgggatc gataccgaca cagacgaggg
     2521 tgtcgacacc gcgaatccga gccgtctcga tcgcctcggc ggggtcgtcg tcgagcagga
     2581 acaggtggca gtgcgtgtcg accgcgccgc tctgcatcgg cggttcaggg ttcgactcgt
     2641 tcacgggccc gacaccctag tcgtcgccac ccatcctcga ccccgactcg aagatccgga
     2701 gcactgccac gctgcgtgca cgagccgctc tcgatcggaa gtcgaggagg tgagtgccac
     2761 gtgaaacgtg atggcgcgaa ccctcactca ggagtcgagc cgagggaaca gcgagtcgcc
     2821 tttgacggtc ttggtcccgg gccgcaaccc gccccaggcg gaagcccccg gcagccgctg
     2881 cgcctccagc ggctcctcga tccccagctg ttcccacaag cgagccgcgg cccccggcat
     2941 gatcggcgcg atcaggatcg ccaagatccg cagtgtctcg gccgacgcgt acaggatgcc
     3001 cgcgagctct gcccgccgca cctcatcgtt ggccaaggtc cacggctgcc gctccaccag
     3061 gtagccgttc gctcgcccga cgatctccca gacggcggcc agcgcctggg tcagggcgag
     3121 cgcggtcatg tgctcgtcgt accgccgggc gacgtcggcg gtcagcgccg ggagatcgtc
     3181 ctcgacgccg ccggcctccg gctcgggaac gagcccgtcg aaggacgcgg cgagcatcgc
     3241 gaggatccgg ctcgcgaggt tgcccagccc attcgcgagg tcggcgttgt ggcgatcgac
     3301 catcgactcc cacgagaagc tcccgtcctg accgaactgg atctcccgca tgaagaagta
     3361 gcggtaagag tccacgccga agtggtccgt gagctcgaag gggtggatgc cggtcgcgtt
     3421 ggtcttcgac atcttctggc cgccgaccgt gaggaacccg tgcgcccaga cctgctcggg
     3481 cggttcgaca ccacccgcca tgagcatggc cggccagtag acggcgtgga accgcaagat
     3541 gtccttgccg atcaggtgga cgttcgccgg ccagatccgg gagaaacgat cctcgtcggt
     3601 cccgtacccg gcggcggtga cgtagttggt gagggcatcg aaccagacgt agcagacgtg
     3661 cttcgggtcc cacgggagcg ggattcccca ccgaaagttc gtccggctga tcgagaagtc
     3721 ctggagcccg cccccgatca gggagaggac ctcgttgcgc cgggtctcgg gttggacggc
     3781 ctcgggacgc cgctcatagt gctcgagcag acggtccgcg taggccgaca ggcggaagaa
     3841 gtagttctcc tcctgaacgt gctcgacggg gatgccgtgg atggggcaca tcccgtcgac
     3901 gagctcgtcc tcggtgtagt aggcctcgca ggacacgcaa tacaggccct cgtagatccc
     3961 gagatagatg tcgtcgcggc cgttgtcgtg aacggccgac agcagacgct ggacggcctt
     4021 ctcgtggcga ggctccgtcg tacggatgta gtcgtcgtag ccgatgtcca ggccggccca
     4081 gacctccctc catcggggcg ccatctcgtc gacccaggtc tgcggatcct tgcctgccgc
     4141 ctcggcccgg cgctggaggt tcagcccgtg ctcgtcggtc ccggtgagga ggaggacctc
     4201 ctcgccccgc agccggtggt accgggcgat gaagtcggcc gccacggagg tgtagccgtg
     4261 accgatgtgc gggtcggcgt tcgggtaata gatcggcgtc gtgatgtaga agacgtcctt
     4321 gcccatggtc cggtcatggt agccgagcct cccacgcagt cgggtcgctc aggggcccgg
     4381 gaagatggcc gcctggtact cggcggtcgg gacgtcgagg cacgggccct cgccgagctc
     4441 ctccagacca gccaggtctc ccgttcccca gtcggtcacg accggcaccg ggctcatcag
     4501 ctggaacgga tcgaggatgt ggccgagccc gaccacgtgg ccgagctcgt gctggagcac
     4561 cgtcccgacc gagggggcga gatcgaaccc gtcctccagt ccggcgctcg cgttgatcac
     4621 gaccgcggcg ctcacgaact gttcggcgtc gaaccgggtc gtgaccggga tcgcgacgcc
     4681 gtacgcgcct gcggcgtcca acgcccgcat cgccgaacgc gaccggaacg cgatcaagac
     4741 cggcgaccag cgcagcccgc cggttgtggt cgtgacgaag tcccccgagc cgatgaggtc
     4801 cccgaccgac acgtggacga ccccgtcgaa ctcgaacgcg agtccgctcg cccggctgac
     4861 ccgacggacc gcctccttca cgtcgtcgag gacgtggtcg tccaccagcc gtccgaggtc
     4921 gacctggtag tggatcgtgg agcagggatc ccatcgatac ggctcgccgt cgtcggagcg
     4981 cccgatgaag gcgtgcgatc tcgcgcctcc gaacagcggc cgcgggtcca ctttcgaccc
     5041 atgcgaggcc agggttggcc cgatcccggc gatgagcacc agcacgagcc cgacggcgac
     5101 cgaggccagc gtgcgcgccg gccggcgggt cacggggacg gccggccgag ccggcgccgc
     5161 ccagggcgcg ggcggcaacg ggaggtccgg tgggtggaga gccggaggcg ggggaagctc
     5221 gggccggttc atccgatcca tccgatcgca ttacccgacg aggttcgtta cccgatcatc
     5281 ggctgatcga accggccgct tgaaccgtcg agcgcgcttc agtccgtctc gatctcggag
     5341 ccgatcagcg ctccgtagat ctcgttcgcc gatccaccgc ctcgtcgggc gaccgcgacc
     5401 gccgcgtcgc gcttgcgcat cccgtccgcg atcaggcggc gcgcctcctc gaccatctcg
     5461 tccaggccgg gcgttcgtgc ctcggtcccc tcgatcacga cgaccatctc gccgcgcggc
     5521 tcggcctcca gcagggacgc gatcacctcg gagatccggc ctcgaacgac ctcctcgtgg
     5581 agcttggtga gctcgcgggc cacgacggcg cggcggtcgc cgagctccac cagggcgtcg
     5641 cgcaggagcg tgatcacgcg cagcggggac tcgaacagga cgacggtccg cggatcgtgg
     5701 gccagcgccc ggagtcgctc gcgccgctcg ctcgggcggt gcgagaggaa gccctcgaac
     5761 acccaacggt cggtgggcag acccgagacc acgagcgcgg ccgtgacggc cgacggaccg
     5821 gggaccacgc ggatctggat ccccgcatcg gcggccgccc gcaggaagcg gaaccccggg
     5881 tcggagacgg cgggcatgcc ggcgtcggac acgagcgcga tccgctctcc gttgcgcagg
     5941 cgatcgagca gctcgtcgat gcgttggcgc tcgttgccct cgaacaggga gagcatccgc
     6001 gccttgacgc cgatgcgctc gagcaggcgc cccgtccggc gcgtgtcctc ggcggcgacg
     6061 agatcgacca tgccgagcgt ttcgcgtgcg cggtcggaga ggtcggcgag gttgccgatc
     6121 ggcgtgccca ccacgtagag gcatccggcc atgtctcgat cgtcccatgc gatccgcgga
     6181 acggaaccgg cgaacgacgt agcccgccgt cggacgccgc tacagtgccg gacgtggccc
     6241 cgtctcgatc gtcggccgga caccgggact actcggcgac gccgctcgcc cgcaagctcg
     6301 ggatccgcga gggctcccgc gtcctggtcg tcggcgcccc gggcggcttc tcgctcgggc
     6361 cggtgccggg cggggtgtcg ttcgtccggt cggcacgggg accactcgac gtggtactcc
     6421 tgttcaccac gaccctggcc gacctccgcc gtcgcttccc cgccgccgtt cgggcgctgg
     6481 acccggccgg caggctctgg gtggcctggc cgaagaaggc cgcggaggtc gacaccgacc
     6541 tcaccttcga gatcgtccag cgcatggggc tggacgcgga gctggtcgac aacaagtcgg
     6601 cctcggtcga cgacgtctac cagggactcc agttcgtgat ccgactgaag gaccgggcca
     6661 agcgcacggc cggacgccgt ccctgaacgg cgtccctgcc gtaccatcgc tcggatgtcc
     6721 acgctcaccc ggcccgcacc accgttcgcg cagcgccctg cgagcgcgtc gctgcggatg
     6781 cacgcgtggg tcaggcgctt caaccggccg gtcgtggcgg tcctggccgt cgggctgatc
     6841 gcgggctacc tgcgcttcgt gcacctcgcg tacccggagc accgcgtctt cgacgagtac
     6901 tactacacga agtccgcgtg catcctgctc ggctactcgg atcagcggtg cgacatcaac
     6961 agcgcggacg agcggttctg gcgcgagaac gaatgggaca ccggcgcgtg ggtgcatccg
     7021 ccgctcggga aatggatgat cgcgttcggc gagctggcgt tcgggacgga gtcgatgggg
     7081 tggcgcgccg ccgccgcggt cacggggacc gcgacggtga tgctgctcgc cgtcatcatc
     7141 cagctgctgt gggcgagtcc gatctggacg ttcacgggcg gtctgctgtt ggccaccgag
     7201 agcctgaacc tcgtccaatc gcgcaccgga acgctcgaca tcttcgtcgc gttctgggtc
     7261 gtgctcgtgt tcgtgttcct cttgctcgac cgacgatgga tcgaccgtcg gacgccgcca
     7321 ccggtcccag cgacggacgg cccgagcgac gcgggggcga gagtccagcc caggcggcga
     7381 ctgccggcgc cgctgtggcg tccctaccgg ttcgccgccg gcgcggccgg aggcgccgcg
     7441 atggccacga agtggtcggg gctgaccgcg ttcgtcacgg cgatcgcgct cgggttcttg
     7501 tgggaagtca tgcggcgcaa gcagttcggc atgtcgaaac cgatcgtcac gacgatcatg
     7561 accgaggggt tcggcttcgt cctcgcgctg ttggtcacgc cggcaattgt ctacgtcgtc
     7621 acctacatcc cgtggttcct gcacttcggg tgggacctcc agcggtgggt caagatgcag
     7681 ggcgatgccg cgcggttcca cgccgacctg aaggcggtga acgatcaggg gcagccgtgg
     7741 cacgcctact tcgcgccggc gtggaagtgg atcctgctcg cccgacccac ctattactac
     7801 ggggccttcg gatcagacgg tgtccgccag gtgatctacg cgcagggcaa cccggcgatc
     7861 ttctggggca gtctgctcgc gatcccctac gtggcgtacg cgtggtggcg cacgcgcgat
     7921 tggcgggcgg ggttcgtgat cgtcacgatc gccgggctct acctgccctg gttcctggtc
     7981 tcacgcccgc agttcttctt ctacgcgacg cccctcacgc cgttcttcgt cctcgcctgc
     8041 gtctacgcgc tccgggacct gtccgagctg cacgtcgccg gttcccggtc gcgtccgttc
     8101 ctccccttcg tcgtcggctt cgtcgccgcg tcggtgatcc tgttcatctg gttctggccg
     8161 atcctgacgg ccgcgccggt gagcgacgcc gccttcaagc tccggtactg gttcacctcc
     8221 tgggcctgac cggcgcgaga tcagctaccg ggtggatcga cccgcatgcg gagcgcgagc
     8281 gagcatcgcg cgccgcgcgg agggccgtgc accgcgtggt acggacggcc gtcgcggacg
     8341 ccggcgagac ccggcgcgcg catcaacgtg accgaaccgg cgcgcgcgtc ccagcgttcg
     8401 agcagctcgc cctcgcgcga cgacc
//
LOCUS       DATBRK010000325         8004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1068770, whole genome shotgun sequence.
ACCESSION   DATBRK010000325 DATBRK010000000
VERSION     DATBRK010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068770"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VIH70_09655"
     CDS             complement(<1..481)
                     /locus_tag="VIH70_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY8116667.1"
                     /translation="MADLMEAVTSLAKRRGLAFQSSEIYGGLRSSWDYGPLGVELRRN
                     IRHAWWRSMVQLRDDVGGLEAAIIMSPKVWVASGHVAGFHDPLVECLNCHQRFRQDHL
                     GDDPTCPNCGQKRFTEPRQFNLMFKTHMGPIEDESSESYLRPETAQGMFVDFAIVQQT
                     "
     gene            complement(594..1019)
                     /locus_tag="VIH70_09660"
     CDS             complement(594..1019)
                     /locus_tag="VIH70_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEY8116668.1"
                     /translation="MRVPEEARGVIQSDALAHVTTIDPDGRPQTTLAWVGLEGDEIVF
                     ATLPDQRKLRNLRRDPRITISIQTDRVTPYGLHEYLVIYGTARVTDGGGPEVLQRLAH
                     TYLGPDITFPPMPDPPHGFVTRVTVERLGGVGPWNEPTG"
     gene            complement(1024..1773)
                     /locus_tag="VIH70_09665"
     CDS             complement(1024..1773)
                     /locus_tag="VIH70_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY8116669.1"
                     /translation="MSTYLHDLDRERLPNHVGIVMDGNGRWARLRGLPRTDGHTAGEE
                     AMWDTVVGANELGLGWLTMFAFSSENWKRPKAEVAFLMGFNRGLLRRRRDELHRMNVR
                     VRFLGRRDWKVPRSVLKEMEISEALTRHNTGMTLTIAFNYGGRVEVVDAVKRLIADHD
                     AGRLKGEKLTPDSISRRLYHADMPDPDLIIRTSGEERISNFLLWQAAYSELYFTPVYW
                     PDFDREHLYEAIRDYQKRSRRFGAIAENDAG"
     gene            complement(1770..2510)
                     /gene="recO"
                     /locus_tag="VIH70_09670"
     CDS             complement(1770..2510)
                     /gene="recO"
                     /locus_tag="VIH70_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY8116670.1"
                     /translation="MPLYKEQGVVLRSIKLGEADKIVTVMTQGSGKVRAVAKGIRKTT
                     SRFGARLEPFTHVSLMVYRGRGSLDTVSQAEIVSAFRSLRGDLGLFAAGETMLEAVDK
                     VAEEHERNVRLFMLLLTGMRALDARPADPAAVAESFLLKLLSLSGFHPALTTCAVCGA
                     PAPERFASSLGGAVCAADADLDAGPASLEALDFLATLGGTGLLEAGDVRADTRVRRES
                     RSLLFGFTEYHLERRMKSLPILARSLTT"
     gene            complement(2628..2999)
                     /locus_tag="VIH70_09675"
     CDS             complement(2628..2999)
                     /locus_tag="VIH70_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8116671.1"
                     /translation="MAKASKASASDHVQMEGFEGHYAELGGTTVGWETYTADADLTPL
                     FVGLPEDRCQCEHWGYVFEGKLVFHTADGDEEFVGGDSYFVGPGHTPSIFAGTSLVEF
                     SPTDRLNQTMEVITRNMEASG"
     gene            complement(3097..3660)
                     /locus_tag="VIH70_09680"
     CDS             complement(3097..3660)
                     /locus_tag="VIH70_09680"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8116672.1"
                     /translation="MGGQTKKGPGANALKREIEVGVVGESRASAEQVFDVLADLRSHL
                     SWAGERQKGTTRLLTLEAPEGPASVGTEFTTTGADPMGRFTDRSVVTEATRPSAFEFV
                     TEAQLVTKRGKVADWTNVHRYELTPSAKGCRITYTIRIARISSLPGALALFNVRLLSG
                     VGRKASQGVARRGLDNLAKVAEERVGV"
     gene            complement(3731..5389)
                     /locus_tag="VIH70_09685"
     CDS             complement(3731..5389)
                     /locus_tag="VIH70_09685"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8116673.1"
                     /translation="MSVETATDPLARGEEALHAGDWETARSVFEGSLAEADTARAHDG
                     LGRALWWLGDSGGALDHRERAYVSYRREGRKADAARIALWLSREYLDAVGNEPASNGW
                     VARARGLLSEHEPGPELGWLEVTLGERAMDPPTIRSHGEAALQIGRRLADPNLEASAL
                     ALVGRAAVLDGDLEGGLASLDEAMTAATAGEVSDPLVFGDVCCIVTRACEEAGELGRL
                     MRWNEVILSYLERHHHAPLMQWCGTCGAEVFLAHGDISTAEQCLVEAIDGLEKTGHRS
                     RCVQPNVKLAELRVLQGRVEEAERLLAGTEDLQEATRAWVSVHRIRGELAVAAALLLR
                     RLNQVGDTVAAIPLLSLLVEVQLDQGLGGDAAASAERLGSITTRSGHPRYVAMFDLAQ
                     ARVALATGDPSARQRLEVALAAFGRLELPLDEARVRIELARTLEEDEPQLATREGRLA
                     VEAFERLGATREADEAAALVRQLGGPARTGPKLVGTLTKREREVLSLLGEGLSNADIA
                     ARLYISTKTAGHHVSSVLAKLHLRSRAEAAAFVLRHLGGVSSER"
     gene            complement(5453..6316)
                     /locus_tag="VIH70_09690"
     CDS             complement(5453..6316)
                     /locus_tag="VIH70_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8116674.1"
                     /translation="MTARTRVPVRRTAKLYIAGEFPRSESGRSYEVFSHDGRLLSRAA
                     RASRKDLRDAVRAARAAQPEWAGKTAYNRGQILYRVAELMEGRRSQFEAELANAGAKD
                     PRTGVDASIDRWVWYAGWADKIGQLLGATNPVAGPYFNFTIPEPTGVVGIVGPPDQAL
                     LGLVSRLAPAIVSGNTAVVLASESSPLPAVSLGEVLATSDVPGGVVNVLTGLTDELVP
                     WLAGHMDVNAVDLTGVPPSSRAEAERLSAENVKRVVRARDVDPFADDAQSPYEITALM
                     EFKTVWHPMGA"
     gene            complement(6313..7800)
                     /locus_tag="VIH70_09695"
     CDS             complement(6313..7800)
                     /locus_tag="VIH70_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8116675.1"
                     /translation="MSDIAKKATSALEEREPVWEYADAPESTDIVKLEPRYGLFIGGE
                     FVDPKSGRWFETISPATEETLAEVADAGPEDVDLAVRTARKASKDRWRKLPGSERAKY
                     LYRIARQLQERAREFAVLETMNGGKPIKESRDVDLPLAAAHFFYYAGWADKLDYAFPN
                     RVPKPIGVAGQIIPWNFPLLMASWKIAPALATGNTVVLKPAETTPLTALLLAQVIQEA
                     DLPPGVVNIITGGGETGAYLVEHEDVDKIAFTGSTEVGKIIRRTVAGTGKRLTLELGG
                     KAANIAFEDAPLDQAVEGVINGIFFNQGHVCCAGSRLLVQEPVFEPLLEKLKDRLQTL
                     RVGNPLDKNTDIGAINSKAQLSKIEELVAAGVDEGAEMYQPECVLPERGFWFRPTLFT
                     NVSQSHRIAREEIFGPVLSILTFRTPAEAVEKANNTPYGLSAGIWTEKGSRILWMAER
                     LKAGVVWANTFNRFDPASPFGGYKESGFGREGGLHGLEPYLELDG"
     gene            complement(7797..>8004)
                     /locus_tag="VIH70_09700"
     CDS             complement(7797..>8004)
                     /locus_tag="VIH70_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116676.1"
                     /translation="AMRAYEGEPPILLRDREHPNVFKTFGAFPSIEAIEAFRASDLFR
                     GAVDEIRPLLESFQPFTLDEIEWT"
BASE COUNT         1175 a         2861 c         2694 g         1274 t
ORIGIN      
        1 aggtctgctg gacgatcgcg aagtcgacga acatcccctg cgccgtctcg ggtcggaggt
       61 aggactcgct cgactcgtcc tcgatggggc ccatgtgcgt cttgaacatc aggttgaact
      121 gccggggctc cgtgaaccgc ttctggccgc agttcggaca ggtgggatcg tcgcccaggt
      181 gatcctggcg gaagcgttgg tggcagttga ggcactcgac gagcgggtcg tggaacccgg
      241 cgacgtgacc gctcgccacc cagaccttcg gggacatgat gatcgcagcc tccaggccgc
      301 cgacgtcgtc gcggagctgg accatcgaac gccaccacgc gtggcggatg ttgcggcgca
      361 gctcgacgcc gaggggaccg tagtcccacg acgagcgcag ccctccgtag atctcgctgg
      421 actggaacgc gagcccccgc cgcttggcga gggacgtgac ggcctccatc aggtcggcca
      481 tggggctcct cctctctccc ggccgctggc tgcggccgct ggcgtgcgct ccagtctacg
      541 ggtcgacccg gccgcgatac gccgatccgc gtggagagat ggcgcgtgcc ggactagccg
      601 gtcggctcgt tccacggccc gaccccgccg aggcgctcga ccgtgacccg cgtcacgaag
      661 ccatggggtg ggtccggcat cggcgggaac gtgatgtcgg gaccgaggta cgtgtgcgcc
      721 agccgctgga ggacctccgg tcccccgccg tccgtgaccc gcgcggtccc gtagataacg
      781 aggtactcgt gtaacccgta cggggtgacc cggtcggtct ggatcgagat cgtgatccgt
      841 gggtcacgtc gcaggttccg aagcttccgc tgatcgggga gcgtcgcgaa gacgatctcg
      901 tcgccctcga gccccaccca ggcgagcgtc gtctgcggtc gaccgtcggg atcgatcgtt
      961 gtgacgtggg cgagcgcgtc gctctggatg accccgcgcg cctcctccgg gacccgcacc
     1021 tgcctaaccg gcgtcgttct cggcgatggc cccgaaccgt cgcgaacgtt tctggtagtc
     1081 ccggatcgcc tcgtacaggt gctcgcgatc gaagtccggc cagtacaccg gcgtgaagta
     1141 cagctcgctg tacgcggcct gccagagcag gaagttgctg atgcgctcct cgccgctggt
     1201 ccggatgatc aggtcgggat ccggcatgtc cgcgtggtac agccgccgcg agatcgagtc
     1261 gggcgtgagc ttctcgccct tcaggcgtcc cgcgtcgtgg tcggcgatca gccgcttcac
     1321 cgcgtcgacg acctcgaccc gccccccgta gttgaacgcg atcgtgagcg tcatcccggt
     1381 gttgtgacgc gtgagcgcct ccgagatctc catctccttg agcaccgagc gcggcacctt
     1441 ccagtcgcgc cgtccgagga agcggacgcg gacgttcatc cggtggagct cgtcccgccg
     1501 ccgacggagc agcccgcggt tgaagcccat caggaacgcg acctcggcct tgggtcgctt
     1561 ccagttctcg ctcgagaacg cgaacatcgt gagccacccc aggccgagct cgttcgcccc
     1621 gacgaccgtg tcccacattg cctcctcgcc tgccgtatgg ccgtcggtcc ggggcagccc
     1681 ccggagcctg gcccatcgac cgttgccgtc catcacgatg cccacgtggt tcggcagacg
     1741 ctcgcggtcg aggtcgtgga ggtaggtgct cacgtcgtca ggctcctggc gaggatcggc
     1801 aggctcttca tgcggcgttc gaggtggtac tcggtgaagc cgaacagcag cgaacgggac
     1861 tcgcgtctca ccctcgtatc ggcccggacg tcgcccgcct caagcagccc ggtcccgccg
     1921 agcgtcgcga ggaagtcgag cgcctcgagc gaagccggtc cggcgtccag gtcggcgtcc
     1981 gcggcgcaga ccgcgccccc gaggctgctg gcgaagcgct cgggggccgg ggcgccgcag
     2041 acggcgcagg tcgtcagcgc cgggtggaac cccgacagcg acaggagctt gagcaggaac
     2101 gattcggcga ccgcggcggg gtcggccggc cgggcgtcga gcgcccgcat cccggtgagc
     2161 agcagcatga acaggcggac gttccgctcg tgctcctcgg cgaccttgtc gacggcctcc
     2221 agcatggtct cccccgcggc gaacagaccg aggtcgcccc ggagcgaccg gaaggcggag
     2281 acgatctcgg cctggctgac ggtgtcgagc gaacctctcc cccggtagac catgagggaa
     2341 acgtgcgtga agggctccag gcgggcgccg aagcgcgagg tggtcttgcg gatacccttg
     2401 gcgacggccc ggaccttgcc cgagccctgg gtcatgacag tcacgatctt gtcggcctcg
     2461 ccgagtttga tcgagcgcag cacgacgccc tgttccttgt agaggggcac gcggggaatc
     2521 gtagcgccga gcacgtgccg ttcccgtgag cgaacgttgc gaagtcatgg agatgcgcac
     2581 gggccgcccg gaacgggcgg cccctcgctc gatgggcagg tcaggcccta acccgatgcc
     2641 tccatgttcc tggtgatgac ctccatggtc tggttcagcc ggtccgtcgg gctgaactcg
     2701 accagcgagg ttcccgcgaa gatcgacggg gtgtggccgg ggcccacgaa gtaggaatcc
     2761 ccgcccacga actcctcgtc gccgtcggcg gtgtggaaca ccagcttgcc ctcgaacacg
     2821 tagccccagt gctcgcactg gcaacgatcc tccggcaggc cgacgaacag cggcgtcagg
     2881 tcggcatcgg ccgtgtacgt ctcccacccc accgtcgtcc ccccgagctc ggcgtagtgg
     2941 ccctcgaacc cctccatctg gacgtggtcc gaggcgctcg ccttcgatgc cttcgccatc
     3001 gtcattcctc cttgtcggtt gcatcaccga tgagatcgat cgtgatcagg aactcccctt
     3061 gtacgacgac cctcccttcg atcctcgacc ggccgttcag acgccgaccc gttcctccgc
     3121 caccttggcc aggttgtcga gcccccgccg agcgacaccc tgactcgcct ttcgcccgac
     3181 gccggagagc agccgaacgt tgaacagggc gagcgcgccc ggcagggagc tgatcctggc
     3241 gatgcggatc gtgtacgtga tacggcatcc cttggccgat ggtgtgagct cgtaccgatg
     3301 cacgttcgtc cagtcggcga ccttgccgcg cttggtgacg agctgggcct cggtgacgaa
     3361 ctcgaacgcc gacggccggg tcgcctcggt cacgaccgat cgatccgtga acctgcccat
     3421 cgggtcggcg ccggtcgtcg tgaactccgt gcccaccgag gccggcccct cgggcgcctc
     3481 gagcgtgagc agacgcgtcg tgcccttctg ccgctcgccg gcccacgaca ggtggctccg
     3541 aagatccgcc aaaacgtcga agacctgctc ggccgacgcc cggctctccc cgacgacccc
     3601 gacctcgatc tcacgcttga gggcgttcgc ccccggcccc ttcttggtct gtcctcccac
     3661 ggcgcacctc ctgcctcgac gacacgagca gcgtgcgccg tgtgcggcag cgaaggatcg
     3721 ggaggattcc ctatctttcg gacgagacgc cgccgagatg ccgaagtacg aaggcggccg
     3781 cctcggcgcg gctcctgagg tggagcttcg cgaggacgct gctcacgtgg tggcccgcgg
     3841 tcttcgtaga gatgtacaga cgtgcggcga tgtcggcgtt cgagaggccc tcgcccaaca
     3901 gcgagagcac ctcgcgctcg cgcttggtga gcgtccccac gagcttggga ccggtgcggg
     3961 ccggtccgcc caactgcctg accagcgccg ccgcctcgtc ggcctcgcgg gtagctccga
     4021 gccgctcgaa cgcctcgacg gccaggcgac cctcgcgtgt cgcgagctgg ggttcatctt
     4081 cctccagcgt gcgagcgagc tcgatccgca cccgtgcctc gtcgagtgga agctccagcc
     4141 tcccgaacgc ggccagcgcc acctccagcc gctgacgagc cgaggggtcg cccgtcgcga
     4201 gggccacgcg tgcctgcgcg aggtcgaaca tcgcgacgta acgagggtgc cccgagcggg
     4261 tggtgatcga tcccagccgt tcggcggatg ccgccgcatc tccacccaga ccctggtcga
     4321 gctgcacctc gacgaggagc gaaagcagtg ggatggcggc gacggtgtcg cccacctggt
     4381 tgagccgccg gaggagcagc gcggccgcga ccgcgagctc gcccctgatc ctgtgaaccg
     4441 acacccaggc gcgcgtcgct tcctgcagat cctcggtgcc cgcgagcagc cgctcggcct
     4501 cctccacgcg gccctgcaga accctcagct cggcgagctt gacgttcggt tggacacagc
     4561 gagaccgatg cccggtcttc tcgaggccgt cgatcgcttc gacgaggcat tgctcggccg
     4621 tggagatgtc accgtgcgcg aggaagacct cggcgccgca cgtgccgcac cactgcatca
     4681 gcggcgcgtg gtgatgacgt tcgaggtacg agaggatcac ctcgttccaa cgcatcaacc
     4741 gcccgagctc gcccgcctcc tcgcacgccc tcgtgacgat gcagcaaacg tccccgaaga
     4801 cgagcgggtc gctcacctcc ccggcggtcg ccgcggtcat cgcctcgtcg agcgacgcga
     4861 gcccgccctc gagatcgccg tcgagcaccg ccgcccgacc gacgagcgcc agggccgacg
     4921 cctcgaggtt cggatcggcc agcctgcgac cgatctgcag ggcggcctcg ccgtgggacc
     4981 ggatcgtcgg cggatccatt gcccgctccc cgagcgtgac ttccagccat ccgagctcgg
     5041 ggcccggctc gtgttcggac aggaggccac gggcgcgagc cacccacccg ttgctcgccg
     5101 gctcgttgcc caccgcgtcg agatactcgc ggctcagcca gagcgcgatc cgagcggcat
     5161 ccgccttccg gccctcccgg cggtagctga cataggcgcg ctcgcggtga tcgagtgcgc
     5221 cccccgagtc gcccagccac cacagcgctc gaccgagacc gtcgtgggcg cgggcagtat
     5281 cggcctcggc cagcgagccc tcgaagaccg accgagccgt ctcccagtcg ccggcgtgca
     5341 gggcctcctc gccgcgggcc aggggatcgg ttgccgtctc gacggacacg gcccgcatga
     5401 tggcctttcg gtgtggccga tgccaagcgg ggtgccgggg atgagtgcct tctcaggctc
     5461 ccatcgggtg ccagaccgtc ttgaactcca tcagcgccgt gatctcgtag gggctctggg
     5521 cgtcgtcggc gaaggggtcg acgtcgcggg cccggacgac gcgcttgacg ttctccgccg
     5581 agaggcgctc ggcctccgct cgggacgaag gcgggacgcc ggtcaggtcg acggcgttga
     5641 cgtccatgtg cccggccagc cacgggacga gctcgtcggt caggccggtg aggacgttca
     5701 ccacgcctcc agggacgtcc gaggtcgcga gcacctcgcc gagcgaaacc gcgggcagcg
     5761 gggacgactc gctggcaagg accacggccg tgttcccgga cacgatggcc ggcgcgagcc
     5821 gcgacaccag gccgaggagc gcctggtccg gcgggccgac gatcccgacg actccggtcg
     5881 gctcggggat cgtgaagttg aagtacgggc ccgcgaccgg gttcgtcgct cccaggagct
     5941 ggccgatctt gtcggcccac cccgcgtacc agacccagcg atcgatcgag gcgtcgacgc
     6001 ccgtgcgtgg gtccttcgcg cccgcgttgg ccagctcggc ctcgaactgc gagcggcgac
     6061 cctccatcag ctcggccacg cggtacagga tctgtccccg gttgtacgcg gtcttacccg
     6121 cccactcggg ttgcgcggcg cgcgccgccc gaacggcgtc gcgcaggtcc ttccgggaag
     6181 cgcgcgcggc gcgcgagagc agacggccgt cgtgcgagaa cacctcgtac gagcgtcccg
     6241 actcgctccg agggaactcg ccggcgatgt acagcttcgc cgtcctgcgg acggggaccc
     6301 gggtcctggc ggtcatccgt ccagctccag atagggctcg aggccgtgca gcccgccctc
     6361 gcgcccgaac ccgctctcct tgtagccgcc gaacggcgac gcggggtcga accggttgaa
     6421 cgtgttcgcc cacaccacgc cggccttcag ccgctcggcc atccacagga tccgcgagcc
     6481 cttctcggtc cagatcccgg cggacaggcc gtacggcgtg ttgttggcct tctcgacggc
     6541 ctcggccggc gttcggaacg tgaggatcga cagcacaggg ccgaagatct cctcgcgtgc
     6601 gatccggtgc gactgcgaga cgttcgtgaa cagcgtgggg cggaaccaga agccgcgttc
     6661 gggcaggacg cactcgggct ggtacatctc ggcgccctcg tcgacgccgg ccgccacgag
     6721 ctcctcgatc ttggagagct gggccttcga gttgatcgcg ccgatgtcgg tgttcttgtc
     6781 gagcgggttc ccgacgcgaa gcgtctggag gcggtccttc agcttctcga gcagcggctc
     6841 gaagaccggc tcctgcacga gcaaccgcga gcccgcgcag cacacgtggc cttggttgaa
     6901 gaagatgccg ttgatcacgc cctcgacggc ctggtcgagc ggcgcgtcct cgaacgcgat
     6961 gttcgccgcc ttgcccccga gctcgagcgt gagccgcttc ccggtccccg ccaccgtccg
     7021 ccggatgatc ttgcccacct cggtcgagcc cgtgaacgcg atcttgtcga cgtcctcgtg
     7081 ctcgacgagg tacgcgcccg tctccccgcc ccccgtgatg atgttcacca ccccgggcgg
     7141 aagatccgcc tcctggatca cctgcgcgag caggagcgcg gtaaggggcg tggtctccgc
     7201 cggcttcagg acgaccgtgt tccccgtcgc gagcgcgggt gcgatcttcc acgaggccat
     7261 cagcaagggg aagttccacg ggatgatctg acccgccacg ccgatcggct tgggcacgcg
     7321 gttcgggaac gcgtagtcga gcttgtcggc ccagccggcg tagtagaaga agtgcgccgc
     7381 ggcgagcggc aggtcgacgt cgcgcgactc cttgatcggc ttgccgccgt tcatcgtctc
     7441 gagcaccgcg aactcccggg ctcgctcctg gagctggcgc gcgatccggt acaggtactt
     7501 ggcgcgttcg ctcccgggga gcttgcgcca ccgatccttg gaggcctttc gcgcggttcg
     7561 gaccgctagg tcgacgtcct cggggccggc gtcggcgacc tcggcgagcg tctcctcggt
     7621 cgcggggctg atcgtctcga accaccgccc gctcttggga tcgacgaact ctccgccgat
     7681 gaacaggccg tagcgcggct cgagcttgac gatgtcggtc gactccggcg cgtcggcgta
     7741 ttcccagacc ggctcccgct cctcgagcgc ggacgtcgcc ttcttcgcga tgtcgctcat
     7801 gtccactcga tctcgtccag agtgaacggc tggaaggact ccaacagcgg tcggatctcg
     7861 tcgaccgccc cacggaacag gtcagaggcc cggaaggcct cgatcgcctc gatgctcgga
     7921 aacgcgccga acgtcttgaa cacgttcgga tgctcgcggt ccctcaacaa gatcgggggc
     7981 tcgccctcgt acgcccgcat cgcg
//
LOCUS       DATBRK010000326         7212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_203772, whole genome shotgun sequence.
ACCESSION   DATBRK010000326 DATBRK010000000
VERSION     DATBRK010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203772"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VIH70_09705"
     CDS             complement(<1..732)
                     /locus_tag="VIH70_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY8116677.1"
                     /translation="MATRVTTEPSHEALGLTSEDLRSMYRTMVTARLIDEAAFRLNRQ
                     GKAPFVVPVSGHEGCQVGTGWPLRPGQDIWVPYYRDVAIVLIAGMTPYEIFLGLFAKA
                     EDPSSGGRQMPSHWGSRRLGIISHSSPIATQVPHAAGIAYAAKYRGEDAVVGCWFGEG
                     ATSAGAWHEGLNFAGIHKLPVVFVCENNYYAISVGQPLQMAIENVADRAAGYGFPGVV
                     VDGNDVLASFAAMKTAVERARAGEGP"
     gene            932..1207
                     /locus_tag="VIH70_09710"
     CDS             932..1207
                     /locus_tag="VIH70_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY8116678.1"
                     /translation="MYGYTMNKKAVSDRLRRIEGQVRGIQRMVEEDQYCIDILTQVNS
                     ATSALKSVGMGVLDDHVRHCVKESVQSGDTEEKLEELLTAVARFAGK"
     gene            1211..2530
                     /locus_tag="VIH70_09715"
     CDS             1211..2530
                     /locus_tag="VIH70_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEY8116679.1"
                     /translation="MVASVLSPIAEALDFAFAMAWEVLWALVLGFALSAVVQAVISKG
                     EMSRLLPDDSPRSLAIACGLGAASSSCSYAAVALARSIFRKGADFSAAMAFEFASTNL
                     VIELGILLAVLLGWQFTLAEFVGGPIMIVILALLFRAFLRPELVDLARRQADKGVPGV
                     MEGHAEMDMSVGGGSILSRVRSREGFTAISHFYVTDWASIWKDIALGLVIAGALAAWV
                     PESFWQSFFLVDHPLLSKIWGPMIGPVVAMLSFVCSIGNVPLAAVLWNGGISFGGVAA
                     FIFADLIILPILNIYRKYYGRRVSLFLLATFYTAMVAAGLVVEVLFQALGLVPDERNA
                     KVIEASVTWNYTTFLNIAFLALSAVLVVRFLRTGGPQMLRMMNAPAATHPDAHSTHAD
                     HADHASQPAAEDEGYACPMHPEVRGGPDDRCPRCGMRLERRDDSTRS"
     gene            2547..3347
                     /locus_tag="VIH70_09720"
     CDS             2547..3347
                     /locus_tag="VIH70_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8116680.1"
                     /translation="MQEQVTESGPVVEATDQNFLETVIEESRKRPVVVDLWASWCGPC
                     RTLGPILERVAGERGGAFLLAKLDVDANPYTAGQFGVQSIPTVVAFRDGQPIDGFVGA
                     IPEPMVNEFVDKLMPTEAELEAEEALEEELEGHLDDAEQKYREALEVDPTNRDARVGL
                     ARIYAETGREDRARETLAPVLPDPEAERILAMLEVRDWAKLTEPGTLASAKRLAARGS
                     WREALDAMLGALTDDPDARQAMLEVFTVLGEDDEMVAEYRRKLAAALF"
     gene            3355..4098
                     /locus_tag="VIH70_09725"
     CDS             3355..4098
                     /locus_tag="VIH70_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8116681.1"
                     /translation="MTGDVHDRIRDYWDRDSDTYDETRSHAISDPLEAAAWRQALADA
                     LPEPGSRVLDVGAGTGALSLLAAELGYEVTALDLSPGMLGRAELKARERGLDDRMRFV
                     VGSGTEAPAGPFDAVMERHVLWTLPDPVGALARWREASSRLVLFEGIWGEDDLRRRAM
                     DLAAEWLRRFMRVPDDHHAPYPREVLAELPLARLPSPVPLIDAVYEAGWSAVRIKRLR
                     DVEWAAKLHEPWPLGSLEQRPRYTIVAGA"
     gene            complement(4112..4885)
                     /locus_tag="VIH70_09730"
     CDS             complement(4112..4885)
                     /locus_tag="VIH70_09730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8116682.1"
                     /translation="MSDDRIHPAAGRGFEVSVEHYERGRPGYPDDAVTYLVRELGVGE
                     GRDVLELGAGTGKFTELIVHTGARITTVEPVPAMRAALERNCPTVRVLDGTAEAIPAE
                     DSAFDAVIAAAAFHWFDGPRALPEIHRVLRPEGALGLIWNSRDEASDWSERLTEIFDR
                     LAGEGAPRYRSGRWREALQRTELFGPLHHRLAYHVHHVTPEAFLDRVLSVSYVAAASE
                     GDRARVRAEVDELIATDPELGGDEIAIPYRTDVFWTRRR"
     gene            complement(4882..5583)
                     /gene="lipB"
                     /locus_tag="VIH70_09735"
     CDS             complement(4882..5583)
                     /gene="lipB"
                     /locus_tag="VIH70_09735"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY8116683.1"
                     /translation="MARLIRPHGLVDYDAANAQMHELAERRLAGVAEDTLILLEHPPV
                     YTAGRRWRRDHIVWTEEQIAAAGAGLRFIDRGGSLTFHGPGQLVGYPVLDLGARPDAM
                     RFVRDLEEVVIRAAADVGVGLDRSEVQTGVWSGPSKVCAIGVRLMRMRVSLHGFALNC
                     TTDLSWYDAIVPCGLAGEGVASLSHLAGREITVEEMAPIVARRFEEVFGLSLEPNDEA
                     WPREQIDVLTANATA"
     gene            complement(5588..7066)
                     /gene="sucB"
                     /locus_tag="VIH70_09740"
     CDS             complement(5588..7066)
                     /gene="sucB"
                     /locus_tag="VIH70_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEY8116684.1"
                     /translation="MTAVKMPQLGETVIEGTILKWLKQEGDQVALDEPLFEISTDKVD
                     TEVPSSAAGTLTKILVPEGETVAVGTELAEISEGGAGAAEAPGAASQPATTGSGGAET
                     SSAPAGGDDAPSAAAPPAAPAAQPASAPPAAPAQTPAASPAAASAETVPSQMATSTQS
                     ALPDRGPRSQILSPLVRKLANEHGVDLSQVSGTGTGGRITKNDVLGFVSTQEAMAPVA
                     SAPEAAPVAQPAPAAPATAPATPPAPSTAPAVAQPAPAAMGGGEELVPVSHIRRTIAQ
                     HMKASLEISARAWNAVEINMENVARVRERTKDAFKAREGFGLTYMPFVTRAVTEALLA
                     HPMVNSELRGDEIAVKHFVNMGIAVSYDEGLIVPVLKGSDTMNLLGIARGINDLATRA
                     RAKKLLPDEVHGGTFTITNPGPFGSLISVPIINQPQTGILGFDTVEKRPVVIDDTIAI
                     RHMVYISMSWDHRVIDGALASQFLARVKQNLETWDFAEDLAL"
BASE COUNT         1033 a         2476 c         2582 g         1121 t
ORIGIN      
        1 cggaccctcg cccgcccggg cgcgttcgac cgcggtcttc atcgccgcga acgaggcgag
       61 gacgtcgttc ccgtccacga cgacgcccgg gaacccgtag ccggcggcac ggtcggcgac
      121 gttctcgatc gccatctgca gcggttgccc aacgctgatc gcgtagtagt tgttctcgca
      181 cacgaagacc acggggagct tgtggatccc cgcgaagttc agaccctcgt gccaggcgcc
      241 cgcgctggtc gccccctcgc cgaaccagca gccgaccacg gcgtcctcgc cccggtactt
      301 cgccgcgtag gcgatgcccg cggcgtgggg tacctgcgtg gcgatggggg aggaatggct
      361 gatgatcccc agccggcgcg aaccccagtg gctcggcatc tggcggccac cggaggaggg
      421 atcctcggcc ttggcgaaca ggccgagaaa gatctcgtac ggcgtcatcc cggcgatcag
      481 gacgatcgcg acgtcgcggt agtaggggac ccagatgtcc tgccccggtc ggagtggcca
      541 ccccgtgccg acctggcatc cctcgtggcc ggagaccgga acgacgaacg gcgccttgcc
      601 ctgacgattg agacggaacg cggcctcgtc gatgagccgc gccgtcacca tcgtgcgata
      661 catggagcgg aggtcctcgg acgtgaggcc gagggcctcg tgcgacggtt ccgtcgtgac
      721 tcgggtcgcc acacggcgag tctagcatcg gccctgcgtg atccaggagc cgccgggttc
      781 ctcgtctcgg gctgtcgttc gctctgatct tgcgcgatcg tgtcgagccg ggaacacggc
      841 cgcacggcgg atcggcgcgg gcctgcaggc gttccccgct atacccccct agggtataat
      901 gggtgtgtcg ccaccatcgg gaggaacggc catgtacggg tacacgatga acaagaaggc
      961 ggtctcggac cgcctccggc ggatcgaggg acaggtgcgt gggatccagc ggatggtcga
     1021 ggaggatcag tactgcatcg acatcctgac gcaggtgaac tcggccacct cggcgctcaa
     1081 gtcggtcggc atgggcgtgc tcgacgatca cgtacgtcac tgcgtcaagg agagcgtcca
     1141 gagcggcgac accgaagaga agctcgagga gctcctgacg gcggtcgcgc ggttcgcggg
     1201 gaagtaggcg gtggtcgcgt cggtgctgtc gccgatcgcg gaggcgctcg acttcgcctt
     1261 cgcgatggcg tgggaggtcc tgtgggccct ggtcctgggg ttcgccctgt cggcggtcgt
     1321 gcaggccgtg atctccaagg gcgagatgag ccgcctgcta cccgacgact cgccgaggtc
     1381 gctcgcgatc gcctgcggtc tgggtgctgc gtcgtcctcg tgctcgtacg cggcggtcgc
     1441 gctcgcgcgc tcgatcttcc gcaagggcgc cgatttctcc gcggcgatgg cgttcgagtt
     1501 cgcatcgacc aacctcgtga tcgagctcgg gatcctgctc gcggttctcc tcggctggca
     1561 gttcacgctg gccgagttcg tgggcggccc gatcatgatc gtgatcctcg ccctgctgtt
     1621 ccgagcgttc ctgagacccg agctcgtcga cctcgcacgc cggcaggccg acaagggcgt
     1681 gccgggcgtg atggaaggtc acgccgagat ggacatgtcc gtcggcgggg ggtcgatcct
     1741 gtcgagggtg cgttcgcggg aggggttcac ggcgatcagc cacttctacg tgacggattg
     1801 ggcgtcgatc tggaaagaca tcgcgctcgg tctcgtgatc gcgggcgcgc tcgcggcctg
     1861 ggtgcccgag tcgttctggc agagcttctt cttggtggat caccccctgc tctcgaagat
     1921 ctgggggccg atgatcggcc cggtcgtggc gatgctctcg ttcgtctgct cgatcgggaa
     1981 cgtcccgctc gcggcggtgt tgtggaacgg cgggatcagc ttcggcggcg tggccgcgtt
     2041 catcttcgcc gacctgatca tcctgccgat cctcaacatc taccggaagt actacggtcg
     2101 gcgggtgtcc ctcttcctcc tggcgacgtt ctacacggcg atggtcgcgg ccgggctggt
     2161 cgtggaggtg ctcttccagg cgctcgggct cgtgcccgac gagcggaacg cgaaggtgat
     2221 cgaggcgagc gtgacctgga actacacgac gtttctcaac atcgccttcc tggcgctgtc
     2281 cgccgttctg gtggtgcggt tcctgcgcac cggcggcccg cagatgctcc ggatgatgaa
     2341 cgcgccggcc gcaacgcatc ccgacgcgca ctcgacccac gccgaccacg ccgaccacgc
     2401 gtcccaaccg gccgccgagg acgagggcta cgcctgcccg atgcaccctg aggtccgcgg
     2461 cgggcccgat gatcggtgcc cgagatgcgg gatgcggctc gagcggcgag acgattcgac
     2521 gcgaagctga ctactctggg gtggagatgc aggaacaggt gaccgaatcc gggcccgtcg
     2581 tcgaggcgac cgaccagaac ttcctcgaga ccgtgatcga ggagtcgagg aagcgccccg
     2641 tggtcgtcga cctgtgggcg tcgtggtgcg gaccctgccg gacgctcggc ccgatcctgg
     2701 agagggtcgc cggcgaacgc gggggcgcgt tcctgctcgc caagctcgac gtggacgcca
     2761 acccgtacac ggcgggccag ttcggcgtcc agagcatccc gaccgtggtg gcgttccgcg
     2821 acggacagcc gatcgacggg ttcgtcggcg cgatccccga gccgatggtg aacgagttcg
     2881 tcgacaagct catgcccacg gaggccgagc tggaggcgga ggaggcgctc gaggaggagc
     2941 tcgaggggca cctggacgac gccgaacaga agtatcgcga ggcgctcgag gtcgacccca
     3001 ccaatcggga cgcgcgggtc gggctcgccc ggatctacgc cgagaccggg cgcgaggacc
     3061 gggcacgcga gacgctcgcg cccgtcctgc ccgaccccga ggccgagcgc atcctcgcga
     3121 tgctcgaggt ccgcgactgg gcgaagttga ccgaacccgg gacgctcgcc tccgccaagc
     3181 ggctggccgc acgggggagc tggcgcgagg cgctcgacgc gatgctcggc gccctgaccg
     3241 atgacccgga cgcccgccag gccatgctcg aggtcttcac ggtgctgggc gaggatgacg
     3301 agatggtcgc cgagtaccgc cgcaagctcg ccgccgcgct gttctgaggc agcgatgacc
     3361 ggcgacgtcc acgaccggat ccgcgactac tgggaccggg actccgacac gtacgacgaa
     3421 acgcgctcgc atgcgatctc ggatccgctc gaggccgcgg cgtggcggca ggcgctcgcg
     3481 gatgccctcc cagaaccagg ttcgagggtg ttggatgtcg gcgcggggac gggcgcgctc
     3541 tcgctgctcg cggccgagct cggctacgag gtcacggcgc tcgacctttc acccgggatg
     3601 ctcggccggg cggagcttaa ggccagggag cgcgggctcg acgaccggat gcggttcgtc
     3661 gtgggttcgg ggaccgaggc gcccgcgggg ccgttcgacg cggtcatgga gcggcacgtc
     3721 ctgtggacgc tgcccgaccc cgtgggcgcg ctcgcgcggt ggcgcgaggc gtcgagccgg
     3781 ctcgtgctgt tcgaagggat ctggggcgag gacgacctcc gccggcgggc gatggacctc
     3841 gcggcggagt ggctccgccg gttcatgcgc gtgcccgacg accaccacgc cccgtacccc
     3901 cgggaggtgc tggccgagct gccgctcgcg cggctgccct cgcccgtccc gctgatcgac
     3961 gcggtctacg aggccgggtg gtcggcggtg cgcatcaagc gcctccgcga cgtcgagtgg
     4021 gcggccaagc tccacgagcc gtggccgctc ggctcgctcg aacaacgccc ccgatacacg
     4081 atcgtcgccg gcgcctgacg gcgcgtcgaa ttcaccgtcg gcgggtccag aacacgtccg
     4141 tgcggtacgg gatcgcgatc tcgtcgccgc cgagctcggg gtcggtcgcg atcagctcgt
     4201 cgacctcggc acgaacgcgc gcgcgatcgc cctcggaggc ggccgccacg tagctcaccg
     4261 agagcacgcg atcgaggaac gcctcgggcg tcacgtggtg gacgtggtac gccaggcggt
     4321 ggtgcagcgg tccgaacagc tcggtccgct ggagtgcctc ccgccagcgc cccgatcggt
     4381 accggggcgc gccttcgccg gccagccggt cgaagatctc ggtcaaccgt tcggaccagt
     4441 cgctcgcctc gtcgcgtgag ttccagatca gcccgagcgc gccctcgggg cgcaggaccc
     4501 ggtggatctc gggcagcgcg cgggggccgt cgaaccagtg gaaggccgcg gcggcgatga
     4561 ccgcatcgaa cgccgagtcc tcggcgggga tggcttccgc cgtgccgtcg agcacccgga
     4621 ccgtggggca gttgcgctcc aacgcggcgc gcatcgccgg gacgggctcg acggtcgtga
     4681 tcctcgcgcc cgtgtgcacg atgagctcgg tgaacttgcc ggtccccgcg ccgagctcca
     4741 ggacgtcccg tccctcgccg acaccgagct cacgcacgag ataggtcacg gcgtcgtcgg
     4801 ggtagcccgg tcgcccacgc tcgtagtgct cgacggagac ctcgaatccg cggccggccg
     4861 cggggtggat ccgatcgtcg ctcacgccgt ggcgttcgcc gtcaggacgt cgatctgctc
     4921 ccggggccac gcctcgtcgt tcggctccag gctcagcccg aacacctcct cgaaccgacg
     4981 ggccacgatc ggcgccatct cctcgacggt gatctctcgc cctgcgaggt gggacaggga
     5041 ggcgacgccc tccccggcga gaccgcaggg cacgatcgcg tcgtaccagg acaggtcagt
     5101 ggtgcagttg agcgcgaatc cgtgcagcga gacccgcatc cgcatcagcc gaacgccgat
     5161 cgcgcacacc ttgctcggtc cggaccacac gccggtctgc acctcgctgc gatccagccc
     5221 gacgccgacg tcggccgcgg cccggatcac gacctcttcg aggtcgcgta cgaaccgcat
     5281 cgcgtcgggc cgcgcgccga gatcgagcac cgggtacccg acgagctgtc cgggtccgtg
     5341 gaacgtgagc gagccgccgc gatcgatgaa ccggagcccg gcgccggcgg cggcgatctg
     5401 ttcctcggtc cacacgatgt ggtcgcgccg ccagcgccgg ccggccgtgt agaccggcgg
     5461 atgctcgagc aggatcaagg tgtcctcggc gacgccggcg aggcggcgct cggccagctc
     5521 gtgcatctgg gcgttcgcgg cgtcgtagtc gacgagaccg tggggccgga tcaggcgtgc
     5581 catcgggcta gagcgccagg tcctcggcga agtcccacgt ctcgaggttc tgtttgaccc
     5641 tcgcgaggaa ctgcgacgcg agcgcgccgt cgatcacgcg gtggtcccac gacatcgaga
     5701 tgtaaaccat gtgccggatc gcgatcgtgt cgtcgatcac gaccggacgc ttctcgacgg
     5761 tgtcgaaccc gaggatgccg gtctgcggct ggttgatgat cgggaccgag atcaacgacc
     5821 cgaacggccc cgggttcgtg atcgtgaacg tgccgccgtg gacctcgtcg ggcaggagct
     5881 tcttggcccg cgcccgcgtg gcgagatcgt tgatccctcg cgcgatcccc agcaggttca
     5941 tcgtgtcgga gcccttcagg accgggacga tgaggccctc gtcgtagctc acggcgatcc
     6001 ccatgttgac gaagtgcttg accgcgatct cgtcgccgcg gagctcgctg ttcaccatcg
     6061 ggtgggccag cagcgcctcg gtgaccgcgc gcgtcacgaa cggcatgtac gtgaggccga
     6121 aaccctcgcg cgccttgaac gcgtccttcg tgcgttcgcg cacgcgggcg acgttctcca
     6181 tgttgatctc gaccgcgttc cacgcgcgtg ccgagatctc gagcgaggcc ttcatgtgct
     6241 gggcgatcgt ccgccggatg tgcgagacgg gaacgagctc ctccccaccc cccatcgccg
     6301 cgggcgcagg ctgcgccacg gcgggcgcgg tcgacggcgc gggtggggtc gcgggcgcgg
     6361 tcgcgggtgc ggccggcgcc ggctgggcga ccggggcggc ctcaggcgcg cttgccaccg
     6421 gtgccatggc ctcctgcgta gacacgaagc ccaacacgtc gttcttcgtg atccgccccc
     6481 ccgtcccggt cccactcacc tgcgagagat ccacaccgtg ctcgttcgcc aacttccgaa
     6541 ccagcggcga caggatctgt gagcggggcc cacggtccgg cagcgcgctc tgcgtcgacg
     6601 tcgccatctg cgagggcacg gtctccgccg aagcggccgc cggcgacgcg gcgggcgtct
     6661 gagccggcgc agcgggtggt gccgaagcgg gctgggcggc cggagcggcg ggtggtgccg
     6721 cagcggaggg agcatcgtcg ccaccggcgg gtgcggacga ggtctcggct cctcccgagc
     6781 ccgtcgtagc gggctgcgac gcagctcccg gagcttcggc agcacccgcc ccaccttcgg
     6841 agatctccgc gagctcggtc ccgaccgcca ccgtctcgcc ctccggtacc aggatcttcg
     6901 tcagagtgcc cgccgccgac gacggcacct cggtgtcgac cttgtcggtg gagatctcga
     6961 acagcggttc gtcgagcgcg acctggtcgc cctcctgctt cagccacttc aggatcgttc
     7021 cttcgatcac ggtctcgccg agctggggca tcttgacggc ggtcatcctg cctccggggt
     7081 caccatgcgg acagcttgcg gacggcgtcc tcgatgtcgg acacctgcgg caggtatgcg
     7141 tcctcgagcg gcggagagaa cggcaccggg acgtgaggcg gcgagagccg gacgaccggt
     7201 gcgtcgagcg ac
//
LOCUS       DATBRK010000327         6090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_639351, whole genome shotgun sequence.
ACCESSION   DATBRK010000327 DATBRK010000000
VERSION     DATBRK010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639351"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VIH70_09745"
     CDS             complement(<1..128)
                     /locus_tag="VIH70_09745"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEY8116685.1"
                     /translation="MAEIDRAKIEAAVRMILEGIGDDPDREGVVDTPRRVADMYGE"
     gene            complement(128..793)
                     /locus_tag="VIH70_09750"
     CDS             complement(128..793)
                     /locus_tag="VIH70_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8116686.1"
                     /translation="MAVDRWATFDCYGTLIDWMGGIRDTLSDLWPEHDAELLLSAYHE
                     IEPEVQRGRALPYRQVLAESLQRVAHREGLELADDDRQALGDSLPTWPPFDEVPASLA
                     ELRSRGWRIGILSNTDPEFLAASLEAIGVPVDLEITAAEAGSYKPAHGHWERFFAQTG
                     AERDRHVHVAASLFHDIAPAHELGLRAVWINRLRESSDLPRAAELEDLSKLPETLDDL
                     VSA"
     gene            complement(807..2747)
                     /gene="ftsH"
                     /locus_tag="VIH70_09755"
     CDS             complement(807..2747)
                     /gene="ftsH"
                     /locus_tag="VIH70_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY8116687.1"
                     /translation="MATDPGSPYRRLVRGPAVYVIVVLTAIWVFIAFANRGATSDQLS
                     FTEFQSEVAAGNVDTANFLLGDGVIEGELSDGSPYRVAYVEGTEETLTEDLQKNGVDI
                     SADPQKGSAVLGFLFQLIPVILIVGAFIFIMNQSQGGGGRVMQFGKARAKLVTKDQPK
                     TTFADVAGVDEAIEELQEVKEYLANPAKFQAMGAKIPRGVLLFGPPGSGKTLLARAVA
                     GEAGVPFYSISGSDFVEMFVGVGAARVRDLFEQAKQSAPAIVFIDEIDAVGRHRGAGL
                     GGGHDEREQTLNQLLVEMDGFDQRTAVILMAATNRPDILDPALLRPGRFDRQIVVDRP
                     DLEGRKGILKVHARGKPFDESVDLTTIARRTPGFTGADLSNVINESALLAARWGKKAI
                     SMKEVEEAIDRVMAGPERKTRVMSEREKRVIAYHEGGHALVAHILPNTDPVHKISVIP
                     RGRALGYTLTLPEEDKFLMTREELADELAMLMGGRVAEELIVGDITTGAANDIERATK
                     VARQMVTEYGMSDTIGPQTLGQKQHEVFLGRDFQTQPDYSDQVAFEIDNEVRRMIDEA
                     HDEALEILQSNRGKLDELATMLVERETLERDEVESFLSDIPKRPQRDLSVRGAGLAVS
                     RRAIRTAGQPNIPGTIPPKPIG"
     gene            complement(2866..3258)
                     /locus_tag="VIH70_09760"
     CDS             complement(2866..3258)
                     /locus_tag="VIH70_09760"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY8116688.1"
                     /translation="MKRLLVLTVLLSLTLAGCARGSSEGSDEELGTIIGSVLLAPTCP
                     VETAESPCPGRPLAGVPVRVVDADGNVRASAVSGDEGAFTIDVAPGSYLLTASIEEDP
                     TGSVKPVRVEVRSGEVVHADVLVDSGIR"
     gene            complement(3308..3925)
                     /gene="hpt"
                     /locus_tag="VIH70_09765"
     CDS             complement(3308..3925)
                     /gene="hpt"
                     /locus_tag="VIH70_09765"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY8116689.1"
                     /translation="MEGRVVLSELENAVDAPSVDAAGDARERDIERILITEQQIQDKL
                     RELGEQITRDYEGRELLLLGVLKGAFVMMADISRHIRIPIEVDFMAVASYGAATKTSG
                     VVRILKDLDHDLEGRHVLLIEDIVDSGLTLNYLLKNLRGRRPASLEVCALLKKTGIQR
                     VHLDVKYVGFEIPPDFVVGYGLDFAERFRNLPYVGLLKPEAYTDV"
     gene            complement(3937..4965)
                     /gene="tilS"
                     /locus_tag="VIH70_09770"
     CDS             complement(3937..4965)
                     /gene="tilS"
                     /locus_tag="VIH70_09770"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY8116690.1"
                     /translation="MRRPPAVARVLGRVTRTAREHEMFRPGDVVVVCVSGGPDSVCLL
                     YSLWYLRRLFRIRLEVFHFDHRLRPDAAMDGAYVARLAERLRVPFHLRSADDAPAKGV
                     SVEAWATVARTNAANDVRRAIGAQTIAEGHTLDDQAETVLMNLIRGGGLEAVGGIWPG
                     GTRHGRSALAQPLLDVERAEVEAFCRALHLRPRVDPTNRDTRQLRNAIRLRVIPAIER
                     ATGREVKRPIARTADLLREDRWELDASAAHAFERVVSGGPDELRFDVAGLLALPEPIA
                     TRVIRLALYRIMGSEDAAPWTREAVGSILDLARGRPGRRRDLSNGSTARRGREYVSLS
                     RTSPESRE"
     gene            complement(4965..6038)
                     /locus_tag="VIH70_09775"
     CDS             complement(4965..6038)
                     /locus_tag="VIH70_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY8116691.1"
                     /translation="MEATIDWSMGSRAAALIDWGTVSQTGRRVGGSGPPTPAVERARM
                     REDLADVVPDAETLIEEFTGLSSAGPRARAWLMSRGEWVNANSRGLQRLLEPLAERVL
                     PEGAPRSELRRKALGAQIGALLGYVSRKVLGQYDAFLPPDDDGLLYFVGPNLVEVERR
                     FRLPRRDFRLWVSIHEVTHRVQFGATPWLRPHLERLIGRYVHTVQIDSREVFAQLRRA
                     VDAVRRGADWRGMNGILLIMNDEQRALFARMQALMTLLEGHATFVMNEVAAGRVDDLD
                     RMRNALHERRRSRGGVEKAFQQAIGFETKAKQYASGERFVRDVVTAVGMDGFNRVWRD
                     ETNLPSPEEIADPARWVGRVAKG"
BASE COUNT          891 a         2179 c         2063 g          957 t
ORIGIN      
        1 atctcgccgt acatgtcggc gacccgtcgg ggcgtgtcga ccacgccctc gcggtcgggg
       61 tcgtcgccga tgccctccag gatcatgcgg acggcggcct cgatcttcgc gcggtcgatc
      121 tcggccatca ggcgctcacc aggtcgtcga gcgtctcggg cagcttcgac aggtcctcga
      181 gctcggccgc gcgcggcagg tccgagctct cgcgcaggcg attgatccag acggcccgca
      241 gcccgagctc gtgggccggt gcgatgtcgt ggaacagcga agccgcgacg tgcacgtgcc
      301 ggtcgcgctc ggcgcccgtc tgcgcgaaga accgctccca atgcccgtgc gccggcttgt
      361 acgaaccggc ctcggcggcc gtgatctcga ggtcgacggg gacgccgatg gcctcgagcg
      421 aggcggcgag gaactcgggg tccgtgttcg acaggatgcc gatccgccaa ccgcgcgacc
      481 ggagctcggc cagtgaggcc ggaacctcgt cgaacggcgg ccaggtcgga agggagtcgc
      541 cgagcgcctg gcggtcgtcg tcggcgagtt cgaggccctc gcggtgcgcc acgcgttgga
      601 gcgactcggc gagcacctgc cggtagggca acgcccggcc gcgctgcacc tccggctcga
      661 tctcgtggta cgccgacagc aacagctcgg cgtcgtgctc gggccacagg tcggacaggg
      721 tgtcgcggat cccgcccatc cagtcgatca acgtcccgta gcagtcgaac gtcgcccagc
      781 ggtccacggc catcgcgccg cgaagcctag ccgatgggct tgggcgggat ggtgcctggg
      841 atgttcggct gcccggccgt ccggatcgcc cgccgggaga cggcaaggcc ggcgccgcga
      901 acggacagat cacgctgcgg gcgcttgggg atgtcggaca ggaagctctc gacctcgtcg
      961 cgctcgaggg tctcgcgctc gaccaacatc gtcgcgagct cgtcgagctt gccgcggttg
     1021 ctctgcagga tctcgagcgc ctcgtcgtgc gcctcgtcga tcatccggcg aacctcgtta
     1081 tcgatctcga atgcgacctg gtccgagtag tcgggctggg tctggaagtc tcgcccgagg
     1141 aagacctcgt gctgcttctg cccgagcgtc tgcgggccga tcgtgtccga catcccgtac
     1201 tcggtcacca tctgccgggc caccttggtc gcccgctcga tgtcgttcgc ggcaccggtc
     1261 gtgatgtcac cgacgatgag ctcctccgcg acgcggccgc ccatgagcat cgcgagctcg
     1321 tccgcgagct cctcgcgggt catcaggaac ttgtcctcct ccggcagcgt gagcgtgtac
     1381 ccgagcgcgc ggccccgcgg gatcacgctg atcttgtgga ccgggtcggt gttcggcaga
     1441 atgtgggcca cgagggcgtg cccgccctcg tggtaggcga tcacgcgctt ctcgcgctcg
     1501 ctcatgacgc gcgtcttgcg ttcggggccg gccatcacgc ggtcgatcgc ctcctcgacc
     1561 tccttcatcg agatcgcctt cttgccccag cgagcggcga gcaacgccga ctcgttgatc
     1621 acgttcgaca ggtcggcgcc cgtgaacccg ggagtgcgac gggcgatcgt cgtgaggtcg
     1681 acgctctcgt cgaacggctt gccccgcgcg tgcaccttca ggatcccctt gcgtccctcg
     1741 aggtcgggtc ggtccacgac gatctggcgg tcgaagcgtc cgggccgcag cagagcgggg
     1801 tcgaggatgt cgggccggtt ggtggcggcc atcaggatca ccgcggtccg ctggtcgaac
     1861 ccgtccatct cgacgaggag ctggttgagc gtctgctcgc gttcgtcgtg cccaccgccg
     1921 aggccggcgc cgcggtggcg accgacggcg tcgatctcat cgatgaacac gatcgcgggc
     1981 gcgctctgct tcgcctgctc gaacaggtcc cggacccggg cggccccgac gccgacgaac
     2041 atctcgacga agtccgaccc cgagatcgag tagaagggga cgccggcctc cccggccacg
     2101 gcccgcgcga gcagcgtctt tcccgacccc ggcggcccga acagcaggac gccccgcggg
     2161 atcttggcgc ccatcgcctg gaacttggcc gggttggcca ggtactcctt gacctcctgc
     2221 agctcctcga tcgcctcgtc gacgcccgcg acgtcggcga acgtggtctt gggctggtcc
     2281 ttcgtgacca gcttcgcccg cgccttgccg aactgcatca cgcgcccgcc gccgccctgc
     2341 gactggttca tgatgaagat gaacgcgccg acgatcagga tcacggggat cagctggaac
     2401 aggaacccga gcacggccga tcccttctgc gggtcggccg agatgtccac gccgttcttc
     2461 tggagatcct cggtgagcgt ttcctcggtt ccctcgacgt aggcgacgcg gtacgggctc
     2521 ccgtcggaga gctcaccctc gatcacgccg tcgcccagca ggaagttcgc ggtgtcgacg
     2581 ttgccggcgg cgacctcgct ctggaactcg gtgaacgaga gctggtcgga ggtcgcgccg
     2641 cggttggcga acgcgatgaa gacccagatg gccgtgagga cgacgatgac gtagaccgcg
     2701 ggaccacgga cgagccgccg gtagggcgac ccggggtcgg tagccagggg atgtgctcct
     2761 ttcgaaacct gcggaccgag gagacgctac ggggcccgcg cgtctcccga cggcccgagc
     2821 cctgcgcgat ggtagcacct gggttggctt gtgccgatac cccgatcagc ggatgccgct
     2881 gtccaccagc acgtccgcat gcacgacctc gcccgaacgc acctccaccc ggaccggctt
     2941 caccgacccc gtgggatcct cctcgatcga cgcggtcaac aggtagctcc ccggtgccac
     3001 gtcgatcgtg aacgcgccct cgtcgccact gaccgcgctc gcccgcacgt tgccgtctgc
     3061 gtccacgacg cggaccggca ccccggcgag cggacggccc ggacacgggc tctcggcggt
     3121 ctccaccgga cacgtcggcg cgagcaggac cgaaccgatg atcgtgccga gctcctcgtc
     3181 cgaaccctcg ctcgatcccc gagcacaccc ggcgagcgtg agcgagagga gtacggtgag
     3241 gacgaggaga cgcttcacga cgcttggacg cgccgggagc cgctcgcgtt cctgtgcctt
     3301 cttcgactca cacgtcggtg tatgcctcgg gcttgaggag cccgacgtac gggaggttgc
     3361 ggaaccgttc ggcgaagtcg agaccgtacc cgacgacgaa gtccggcggg atctcgaagc
     3421 ccacgtactt cacgtccagg tggacgcgct ggatcccggt cttcttcaac agcgcgcaca
     3481 cctcgaggct cgctgggcga cggcctcgaa ggttcttcag cagatagttc agcgtgagcc
     3541 cggagtccac gatgtcctcg atcagcagga cgtgacgccc ctcgagatcg tggtccaggt
     3601 ccttgaggat ccgcacgacg cccgaggtct tggtggcggc cccatacgac gcgaccgcca
     3661 tgaagtcgac ctcgatcggg atccggatgt ggcgcgagat gtcggccatc atcacgaagg
     3721 cgcccttgag gaccccgagc agcaggagct cgcgcccctc gtagtcgcgc gtgatctgct
     3781 cgccgagctc gcggagcttg tcttggatct gttgctcggt gatcaggatc cgctcgatgt
     3841 cccgttcccg cgcatcgccc gccgcatcga cggacggcgc gtcgacggca ttctcgagct
     3901 cggacagtac gacccggcct tccatctcgc cccccttcac tcccggctct cgggggaagt
     3961 gcgagagagg gaaacatact cccgcccgcg tctcgccgtc gatccgttcg agaggtcccg
     4021 gcgcctgccg ggacggcccc gcgccagatc caggatcgat cccaccgcct cccgcgtcca
     4081 gggcgccgcg tcctccgacc ccatgatccg gtacagggcc agacggatga cccgggtggc
     4141 gatcggttcg ggcagcgcca gcagaccggc gacgtcgaac cgcagctcgt ccggtccccc
     4201 gctcacgacc cgctcgaacg cgtgggcggc ggatgcgtcc agctcccacc gatcctcgcg
     4261 cagcaggtcg gcggtccgcg cgatcggccg tttgacctct cgcccggtgg cgcgctcgat
     4321 cgccgggatc acgcgcagac ggatcgcgtt gcgcaactgc ctcgtgtccc ggttcgtcgg
     4381 gtcgacccga gggcggaggt ggagtgcccg gcagaacgcc tcgacctccg cccgctccac
     4441 gtcgagcagc ggctgcgcga gcgccgacct gccgtgcctc gtccctccgg gccagatgcc
     4501 gccgaccgcc tcgagcccgc cgccgcggat caggttcatc aggacggtct cggcctggtc
     4561 gtcgagggtg tgtccctcgg cgatcgtctg tgccccgatc gcgcggcgga cgtcgttcgc
     4621 ggcgttcgtg cgcgcgacgg tcgcccacgc ctcgacggag acccccttgg ccggagcatc
     4681 gtcggccgac cggaggtgga aggggacgcg caaccgctcc gcgagccgtg cgacgtacgc
     4741 gccgtccatc gcagcgtcgg gccggagccg atgatcgaag tggaagacct cgagccggat
     4801 ccggaacaac ctgcggaggt accacaggga atagagcagg cacaccgaat ccgggccgcc
     4861 ggagacgcag accaccacca catcccccgg ccggaacatc tcgtgctcgc gggccgtcct
     4921 cgtcacccgt ccgagcacgc gggcgacggc cggcgggcgg cgcatcagcc cttcgcgacc
     4981 ctccccaccc accgggccgg atccgcgatc tcctcggggc tcggcaggtt ggtctcgtcc
     5041 cgccagacgc ggttgaagcc gtccatcccc accgcggtga cgacgtcgcg gacgaagcgc
     5101 tcacccgaag cgtattgttt cgccttggtc tcgaagccga tcgcctgctg gaacgccttc
     5161 tccacccccc cgcgggagcg tcggcgttcg tgcagcgcgt tccgcatccg gtcgagatcg
     5221 tcaacgcggc cggcggccac ctcgttcatc acgaaggtgg cgtgcccttc gagcagggtc
     5281 atcagggcct gcatgcgtgc gaacagcgcc cgctgctcgt cgttcatgat cagcaggatc
     5341 ccgttcatgc cgcgccagtc cgcgccgcgc cggaccgcgt cgacggcccg ccggagctgg
     5401 gcgaacacct cgcgagaatc gatctggacg gtgtgcacgt agcgaccgat cagcctctcc
     5461 aggtggggac ggagccaggg ggtcgcgccg aattgcacgc ggtgggtcac ctcgtggatc
     5521 gagacccata accggaagtc gcgacgcggt agccggaacc gacgctcgac ctcgaccagg
     5581 ttcgggccga cgaagtagag cagcccgtcg tcgtcgggcg gcaggaacgc gtcgtactgc
     5641 ccgagcacct tgcgtgagac gtagccgagg agtgcgccga tctgcgcgcc aagagccttg
     5701 cggcgcagtt cggatcgggg cgccccctcg gggaggaccc gttcggccag cggttcgagg
     5761 aggcgttgga ggcccctgga gttcgcgttc acccactccc cgcgagacat gagccacgct
     5821 cgtgcccggg ggccggcgct cgacagtccc gtgaactcct cgatcagcgt ctcggcgtcg
     5881 ggcacgacat ccgcaaggtc ttcccgcatc cgggcgcgtt cgaccgctgg ggtgggcggc
     5941 ccgcttcctc cgacccgtcg cccggtctgg ctgaccgttc cccagtcgat gagcgcggcg
     6001 gcgcgagaac ccatggacca gtcgatggtg gcctccatgg ccggagaggg cctagccctc
     6061 ggccttcttg cgggctgcga tcatcgccgc
//
LOCUS       DATBRK010000328         3841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2222068, whole genome shotgun sequence.
ACCESSION   DATBRK010000328 DATBRK010000000
VERSION     DATBRK010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222068"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /gene="carB"
                     /locus_tag="VIH70_09780"
     CDS             <1..814
                     /gene="carB"
                     /locus_tag="VIH70_09780"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010838882.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEY8116692.1"
                     /translation="DERIWVLEANPRASRTVPFVSKVTGVPLAKVATSVMLGRTLAEL
                     RDEGLVPAAPRHYLGLPHTAVKAAVLPFGRFPGIDTLLGPEMRSTGEVMGIDADPGRA
                     LAKALVASGASLPREGTIFLSVANRDKRSILYPAKRLADLGFRLLATRGTAAVLERAG
                     IAVSRVSKVSEGGRSVVDLIRDAEVDLVVNTPFGRGPRTDGSLIRAAAAQAGIPCITT
                     LPAVVAALRGIESVRGGGGEPRSLQEYHASADAAVAVQEALVFGHDPAEVAS"
     gene            840..1715
                     /locus_tag="VIH70_09785"
     CDS             840..1715
                     /locus_tag="VIH70_09785"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY8116693.1"
                     /translation="MRRVRAEVLNAKRVGAYHSITIVAPEVAERVRPGQFVSVAVPQG
                     RTFLLRRHFSIHQASRRGGWAGTLEFVFDPRDGDGSSWLAEVKAHQFLDVIGPLGTAF
                     AYPAKLSTCLLVAEGHGSAPMYFLGQELRARGKRVDMLVGAETQELVFKPIEGKRLAQ
                     TIAIATQDGSLGETGGVADLLAQVVEATGAQVIYAAAGRQTLRRISEFCSERQIPSQV
                     AVEEEMACGFGLCFTCTVPVIRKDGSGYDNLRACIDGPVFNPARILWDRWMGEEQRMI
                     GTPPEGFPMVTTWPG"
     gene            1732..2670
                     /locus_tag="VIH70_09790"
     CDS             1732..2670
                     /locus_tag="VIH70_09790"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY8116694.1"
                     /translation="MRPGLGVDLNGLRFTQPVLSASGCLATGHDVPGLVDLRQLGGVV
                     TRSLTFGPSKGWATPRAAETSSGLLTAVGYQNPGVQAFVEEDLPSLRDADVPVVASVA
                     GGSLGEYVNVASSLHLKPGVVALEVCVSCPDDEREGQPFYVRPERLTEVVGAVARLSR
                     VPVFAKLPPLLPGLVETAQACVRAGAFGLTLIAQVPALAVDPHRLRTRTATPVGGLSG
                     PAIKPLALAAVYQVARALPNVPVMGVGGIATAEDAIEFLLAGAWAVQVGTALLVDPSV
                     HVDIAQGILGYLREKGFASPDELRGRVRVLDAGSGP"
     gene            2667..3350
                     /gene="pyrF"
                     /locus_tag="VIH70_09795"
     CDS             2667..3350
                     /gene="pyrF"
                     /locus_tag="VIH70_09795"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY8116695.1"
                     /translation="MIPRPDNPLIVALDVSELDAAEAMARRLEGQAGMLKVGLELFAA
                     NGPVAVSRMRAFARVFLDVKLHDIPATVEGAARNCARLGAAMLTVHALGGEAMVAAAV
                     RGAARGAEDAGHAAPMVLAVTVLSSLAGEGLASAPSLAFEAVAAGADGVVVSGEDVEQ
                     VREVLGSAACLVVPGIRPAGSNGHDQVRILTPEEAVERGADYLVVGRPITGSTDPAGV
                     ARAILATVR"
     gene            3462..3776
                     /gene="mihF"
                     /locus_tag="VIH70_09800"
     CDS             3462..3776
                     /gene="mihF"
                     /locus_tag="VIH70_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEY8116696.1"
                     /translation="MPLPVLTDEQRKQALEKAAIARKRRAELKGQLKSGKLMLKDVLS
                     RQDDDTVGKMKVSTVLESLPGVGKVRARKIMERLDISASRRVRGLGAKQRESLLGEFA
                     KK"
BASE COUNT          497 a         1295 c         1452 g          597 t
ORIGIN      
        1 ggacgagcgg atctgggtcc tcgaggcgaa tccgcgcgcg tcgcgtaccg tgccgttcgt
       61 gtccaaggtc accggggtgc cgctcgccaa ggtggcgacg tccgtgatgc tgggtcggac
      121 cctggccgag ctccgggacg aagggctcgt gccggccgcg ccccggcatt atctggggct
      181 gccgcacacg gccgtgaagg cggccgtgct gccgttcgga cgcttccccg ggatcgacac
      241 gctgctcggg cccgagatgc ggtcgacggg ggaggtcatg gggatcgacg ccgatccggg
      301 acgcgcgctc gccaaggccc tggtcgcgag cggcgcctcg ctgccccgcg agggcacgat
      361 cttcctgtcg gtcgcgaacc gggacaagcg gtcgatcctg tacccggcca agcgcctggc
      421 cgatctcggg ttccgcctgc tggcgacgcg gggcacggcc gcggtgctcg aacgtgccgg
      481 gatcgccgtc tcgagggtct ccaaggtctc cgagggtgga cgctcggtcg tcgatctgat
      541 ccgggatgcc gaggtcgacc tcgtcgtcaa cacgccgttc ggtcgcggcc cgcgcaccga
      601 cggatcgctg atccgcgccg ccgccgcgca ggcgggcatc ccgtgcatca ccaccctgcc
      661 tgccgttgtc gcggcccttc gtgggatcga gtcggtgcgt ggcgggggag gggagccccg
      721 ctcgctgcag gagtaccacg cgtccgcgga tgcagcggtc gccgtccagg aggcgctcgt
      781 gttcggccac gatcccgccg aggtggcgtc ctgatgcccg gagctctcga ggccggagcg
      841 tgaggcgggt ccgcgcggag gtcctgaacg ccaagcgcgt cggcgcgtac cactcgatca
      901 cgatcgtcgc gcccgaggtt gccgaacggg tccgtcccgg gcagttcgtc tcggtcgcgg
      961 tgccccaggg ccgaaccttc ctgctgcggc gccacttctc gatccatcag gcgtcgcgtc
     1021 gcgggggatg ggccgggacg ctcgagttcg tcttcgatcc gcgggatggt gatggctcca
     1081 gttggcttgc cgaggtcaag gcgcatcagt tcctcgacgt gatcgggccc ctcggcacgg
     1141 cgttcgcgta cccggcgaag ctctcgacgt gcctgctcgt cgcggaggga catggctccg
     1201 caccgatgta cttcctgggc caggaactcc gggctcgagg caagcgcgtc gacatgctgg
     1261 tgggcgcgga gacgcaggag ctcgtcttca agccgatcga gggcaagcgc ctggctcaga
     1321 cgatcgccat cgcgacgcag gacgggagcc tgggggagac cggaggcgtg gccgacctgc
     1381 tcgcccaggt cgtcgaggcc accggcgcgc aggtgatcta cgcggcggcc gggcgacaga
     1441 cgcttcggcg gatctccgag ttctgttccg aacggcagat cccctcgcag gtggccgtag
     1501 aggaggagat ggcgtgcggc ttcggcctgt gcttcacctg caccgtgccg gtgatccgca
     1561 aggacggctc gggctacgac aacctccgag cgtgcatcga cgggccggtc ttcaatccgg
     1621 cccggatcct gtgggaccgc tggatggggg aggagcagcg catgatcggg acgccgcccg
     1681 aggggttccc catggtcacg acgtggcctg gatgaatcgg ctcgaggtcg tatgaggccg
     1741 gggctcggcg tcgatctgaa cggtctgcga ttcacccaac ccgtgctctc cgcgtcgggg
     1801 tgcctggcga ccggccacga cgtcccgggc ctcgtcgacc tgcgtcagct cggcggcgtc
     1861 gtgacccgca gcctcacgtt cgggccgtcg aagggatggg ccaccccacg cgcggccgag
     1921 acctcgtcgg ggctgctcac ggccgtcggg taccagaacc ccggcgtcca ggcgttcgtc
     1981 gaggaggatc tgccgagcct tcgcgacgcg gacgtccctg tggtcgcgtc ggttgccggc
     2041 ggaagccttg gtgagtacgt caacgtggcg agctcgttgc acctcaagcc gggtgtcgtc
     2101 gcgctcgagg tctgcgtgtc gtgcccggac gatgagcgcg agggccagcc gttctacgta
     2161 cgcccggagc ggctgaccga ggtcgtgggg gctgtggccc gactgtcccg cgtgccggtg
     2221 ttcgcgaagc tgcctccatt gctgccgggg ctcgtcgaga cggcccaggc gtgtgtccgt
     2281 gccggcgcct tcggtctcac cctgatcgcc caggtcccgg cgctggccgt cgacccccat
     2341 cgcctgcgga cgcggacggc gacgccggtc ggtgggctct cgggccccgc gatcaagccc
     2401 ctcgccctcg cggccgtata ccaggtggcg cgggcgcttc cgaacgtgcc ggtgatgggc
     2461 gtcggaggca tcgcgacggc cgaggacgcg atcgagttcc tgctggccgg tgcgtgggcg
     2521 gtccaggtcg gcacggcgct gctggtcgac ccctcggtcc acgtcgacat cgcgcagggg
     2581 atcctcgggt acctccgcga gaaggggttc gcctcgcccg acgagctgcg cggccgcgtt
     2641 cgggtcctcg acgcggggag cgggccgtga tcccgcgccc ggacaacccg ctcatcgtcg
     2701 cgctggacgt ctcggagttg gacgcggccg aggcgatggc ccggcggctc gagggccagg
     2761 ccgggatgct caaggtcggg ctcgagctgt tcgcggcgaa cggtcccgtc gccgtatcgc
     2821 ggatgcgagc gttcgcccgg gtgttcctcg acgtgaagct tcacgacatc ccggccacgg
     2881 tcgagggcgc ggcgcgcaac tgcgcccggc tcggggcggc gatgctcacg gtccatgcgc
     2941 tgggggggga ggccatggtc gcggccgcgg ttcgcggcgc cgccagaggc gccgaggacg
     3001 cggggcatgc ggccccgatg gtcttggccg tgacggtgct gtcgagcctc gcgggggagg
     3061 ggctcgcctc ggcgccgtcg ctcgccttcg aggcggtcgc ggcgggcgcg gacggcgtgg
     3121 tcgtgtccgg cgaggacgtc gagcaggtcc gcgaggtgct cggctcggcg gcgtgtctcg
     3181 tcgtccccgg catccgcccg gccggctcga atggtcacga tcaggtccgc atcctcacac
     3241 ccgaggaagc ggtcgaacgt ggcgccgact acctcgtcgt cggccggccg atcacggggt
     3301 cgacggaccc ggcgggcgtc gcgcgcgcga tcctcgcgac ggttcgctga cggcgccgcc
     3361 catcggaaac ctgcaggtag agcgcatgag tcgcgttgat gccctcgtgg ggccctggct
     3421 atactcgcgg acttccggcc cccgagcgaa ggagtggtgt gatgccccta cccgtcctga
     3481 ccgatgaaca gcgcaagcag gccctggaga aagccgcgat cgcgcggaag aggcgcgccg
     3541 agctgaaggg tcagctgaag tccggcaagc tgatgctgaa ggacgtgctg tccagacagg
     3601 acgacgacac ggtaggcaag atgaaggtct ccaccgtcct cgagtcgctc ccgggtgtgg
     3661 ggaaggtccg ggcgcgcaag atcatggagc gtctggacat ctccgccagc cgacgggtcc
     3721 ggggtctcgg cgcgaagcag cgcgagtcgc tcctgggcga gttcgcgaag aagtagccac
     3781 cgctggccgc cgggctcggg aggctgttcg tcgtcgccgg accctccggg gtcggcaagg
     3841 g
//
LOCUS       DATBRK010000329         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1934352, whole genome shotgun sequence.
ACCESSION   DATBRK010000329 DATBRK010000000
VERSION     DATBRK010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934352"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VIH70_09805"
     CDS             complement(<1..368)
                     /locus_tag="VIH70_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8116697.1"
                     /translation="MSTNGTALLDVREMSKYFGSVIALKDVSMRCEAGQVTCLLGDNG
                     AGKSTLIKTLSGVYRPDEGEYLFDGRPREFGSPRDALELGIATVYQDLAMIPLLSIWR
                     NFFLGSEPTKGWGPFRRYDVR"
     gene            complement(365..1435)
                     /locus_tag="VIH70_09810"
     CDS             complement(365..1435)
                     /locus_tag="VIH70_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8116698.1"
                     /translation="MAAETIPPAAAPADERVRQKSTLSKVLSRPEVGALAGVVIVWVL
                     FAIVASQHNFISWATTASILNRAAPLGILAVAVALLMIAGEFDLSVGSIIGFSGMVIM
                     VLVTPTTGGGFGWTLWPAVGISLLFALTTGLFNGTIVIATKLPSFIVTLGTLFAFRGV
                     TIAVSRLLTDRTQLGQLDVVAGYGLAQKIFASNLNLFGSQFNVAILWWVGLTALATWV
                     LLRTRAGNWIFGTGGAVDAARNVGVPTRRVKIALFMTTAFAAWLVAVIQVVQFTGADT
                     LRGTGQEFQAIIAAVVGGCLLTGGYGSAIGASLGALIFALVQQGIVITGVEGDWFQVF
                     IGGILLGAVIFNNFIRTRAALR"
     gene            complement(1508..>1969)
                     /locus_tag="VIH70_09815"
     CDS             complement(1508..>1969)
                     /locus_tag="VIH70_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8116699.1"
                     /translation="VALDLRCKGFSEGLGFKAEVIPVDLNDPGGITQSIASKIQSDPD
                     IDGILTLGPTASTPAIQALQQAGELGKIKLATFDLSTDVLNAIDKGDMLFAIDQAQYL
                     QGYLAIVFMTQYLATGAVALGSADRVVLTGPQFVTKDNAADVIKFTDEGVR"
BASE COUNT          347 a          680 c          640 g          302 t
ORIGIN      
        1 cgcacgtcgt accgtcggaa cggtccccat cctttcgtgg gctcggagcc gaggaagaag
       61 ttccgccaga tcgagaggag tgggatcatc gccaggtcct ggtacaccgt ggcgatcccc
      121 agctcgagcg cgtcgcgagg cgacccgaac tcccggggcc tgccgtcgaa caggtactcg
      181 ccctcgtcgg gccggtacac cccggacagg gtcttgatga gggttgactt cccggcgccg
      241 ttgtcgccga gcaggcaggt cacctggccc gcctcgcacc tcatggaaac gtccttcagc
      301 gcgatgacgc tgccgaaata cttcgacatc tcccggacgt ccaggagcgc cgtcccgttc
      361 gtgctcatcg cagcgccgcc ctcgtccgga tgaagttatt gaagatcacc gctccgagca
      421 ggatgccgcc gatgaagacc tggaaccagt cgccttccac gccggtgatg acgatgccct
      481 gctggaccag tgcgaagatc aacgccccga gcgaggcacc gatcgcggac ccgtagccgc
      541 cggtcagaag gcaccccccc acgacggcgg cgatgatggc ctggaactcc tgcccggtcc
      601 cccgaagcgt gtcggcgccg gtgaactgga cgacctggat caccgcgacc agccacgcgg
      661 cgaacgcggt ggtcatgaac agcgcgatct tgacccgccg cgtgggcacg ccgacgttcc
      721 gagcggcatc cacggcgccc ccggtgccga agatccagtt ccccgcgcga gtccggagca
      781 ggacccacgt cgcgagcgca gtgaggccga cccaccacag gatcgcgacg ttgaactggc
      841 tgccgaacag gttcaggttg ctggcgaaga tcttttgggc cagcccgtag ccggcgacga
      901 cgtccagctg gcccagctgg gtccgatccg tgagcaggcg gctcacggcg atggtgaccc
      961 ctcggaacgc gaacagcgtg ccgagggtga cgatgaacga cggaagcttc gtcgcgatca
     1021 cgatcgtgcc gttgaacagg ccggtggtca gggcgaacag caaggagatc ccgaccgccg
     1081 gccacagggt ccacccgaag ccaccgccgg tcgtcggcgt gaccaggacc atgatcacca
     1141 tcccggagaa cccgatgatc gagccgaccg acaggtcgaa ctcgccggcg atcatgagca
     1201 gcgcgacggc cacggccagg atgccgagcg gcgccgccct gttgaggatc gaggccgtgg
     1261 tggcccagct gatgaagttg tgttggctgg ccacgatagc gaagaggacc cacacgatca
     1321 ccaccccggc cagcgccccc acctcgggcc gggacaacac cttcgacagc gtgctcttct
     1381 gccggacccg ctcgtccgcc ggagcggcgg ccggcgggat cgtctccgct gccatgactc
     1441 ctctcctccg atcgcgcgag ccggtgatag ggcccgccga aacggcgggc cccatgaccg
     1501 gattggctta ccgtactccc tcgtcggtga acttgatcac gtcggccgcg ttgtccttgg
     1561 tcacgaactg aggcccggtc agcacgaccc ggtccgcgct ccccagcgcc acggcaccgg
     1621 tcgcgaggta ctgggtcatg aagacgatcg cgagataccc ctgcaggtac tgggcctgat
     1681 cgatggcgaa cagcatgtca cccttgtcga tggcattcag cacgtccgtc gacaggtcga
     1741 acgtggccag cttgatcttc cccagctcgc ccgcctgctg cagcgcctgg atggcgggcg
     1801 tggaggcggt cggtcccagc gtgaggatcc cgtcgatatc gggatctgac tggatcttgc
     1861 tcgcgatcga ctgcgtgatg ccgcccgggt cgttcaggtc gacggggatg acctcggcct
     1921 tgaagcccag cccctcgctg aagcccttgc atcgaaggtc gagggcgac
//
LOCUS       DATBRK010000330         3573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1793578, whole genome shotgun sequence.
ACCESSION   DATBRK010000330 DATBRK010000000
VERSION     DATBRK010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793578"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VIH70_09820"
     CDS             complement(<1..1209)
                     /locus_tag="VIH70_09820"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY8116700.1"
                     /translation="MRLRTLDDLAGDLAGRRVFVRLDLNVPLDDGVVTDDARIRAGLP
                     TLTELLDRGASIVAASHLGRPRGRVDEALRLRPVAELLRRLLDRVVEYVPTDGVLSPE
                     VERSCQALTIEPGRLVLLENLRFDPGEEANDRAFAQRLAGLADLYVDDAFGAVHRAHA
                     SVVALPALVREHGGETAAGRLLQREVEALSRLVADPARPYVAVLGGAKVSDKLGTIGA
                     LVERVDALLIGGAMAFTFLAADGGRVGGSLVELERFDEVRAARARADERGVVVQLPED
                     VVAAASRRVDAPRHTVPSTQVPAGELGLDIGPRTVEEFARTIADAKTIVWNGPMGVFE
                     LEPFSGGTRGVATAIAGASAYSVVGGGDSLAAVKKYGLEGGFDHLSTGGGASLEFIEG
                     RELPGIAVLEV"
     gene            complement(1216..2226)
                     /gene="gap"
                     /locus_tag="VIH70_09825"
     CDS             complement(1216..2226)
                     /gene="gap"
                     /locus_tag="VIH70_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY8116701.1"
                     /translation="MSVKVGINGFGRIGRNFFRAARKLGVDWDFVAVNDITDAPTLAH
                     LLKYDSVLGRFDGEVGTFDGGVTVDGDELRVLAVRDPVELPWKELGAQIVIESTGLFT
                     DRERANKHIEAGAEKVVISAPAKGEDLTVVLGVNDAAYDPSQHNVISNASCTTNCVAP
                     MAKVLDDAFGLEMAFMTTIHAYTNDQAILDLPHKDLRRARAAAINIIPTSTGAAKATG
                     LVLPHLQGKLDGIAMRVPVPDGSVTDLVATLKREASVDEINDAYRSAADRGPLSGGRL
                     VYTEDPIVSSDIVGSPASCTFDAASTMTMGRVAKVIGWYDNEWGYSNRLVDLVDLIAR
                     SL"
     gene            complement(2305..3297)
                     /gene="rapZ"
                     /locus_tag="VIH70_09830"
     CDS             complement(2305..3297)
                     /gene="rapZ"
                     /locus_tag="VIH70_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY8116702.1"
                     /translation="MTAQPERTGAARKPSDVPNSGSAEEPSKLSDRRKRAAKRAAVPG
                     PANQGFTIITGLSGAGRSEAARCLEDLGYFVVDNLPPALLPKMAELGSRPGGPGRVAI
                     VLDVRGGVFFGELSKALQELTELSIPSRILFLEASDGDLVNRYESTRRRHPLAPADRV
                     VEGIRKERMMMEALRGDADLIIDTSGLTPHELRDRIRDAFSDAPPEESLQVSLVSFGY
                     KYGAPRDADLVIDCRFLPNPHWVEELRPLPGTDDRVRTFVRGQATYREFLRRLRALLG
                     FMVPGFVAEGKSYLTVAIGCTGGRHRSVVVVEDLASFFLDKGLPASVDHRDLDR"
     gene            complement(3294..>3573)
                     /locus_tag="VIH70_09835"
     CDS             complement(3294..>3573)
                     /locus_tag="VIH70_09835"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY8116703.1"
                     /translation="VLQHLRDEAHRFAVTYHREKRGKRALASPLDDVAGVGPSRKKAL
                     LKRFGSVARISRASEDEIASTPGVGPDLARAIHDRLTGREPAGGRRSA"
BASE COUNT          511 a         1298 c         1244 g          520 t
ORIGIN      
        1 gacctccagc acggcgatgc cgggcagctc gcgcccctcg atgaactcga gcgaggcgcc
       61 tccgccggtc gagaggtggt cgaacccgcc ctccagccca tatttcttca cggccgcgag
      121 gctgtcgcca ccgccgacga ccgagtacgc gctcgcgccg gcgatcgccg tggctacccc
      181 ccgggtcccg ccgctgaacg gctcgagctc gaagaccccc atcggaccgt tccagacgat
      241 cgtcttggcg tcggcgatcg tcctcgcgaa ctcctccacc gtccgcggcc cgatgtcgag
      301 gccgagctcc cccgccggca cctgcgtgct gggcaccgtg tggcgagggg cgtccacgcg
      361 cctggaggcg gcggcgacca cgtcctcggg cagctgcacc acgacgccgc gctcgtccgc
      421 ccgggctcga gctgctcgca cctcgtcgaa cctctcgagc tcgaccaggc tgccccccac
      481 ccggccgccg tccgcggcga ggaacgtgaa cgccatcgcc ccgccgatca ggagcgcgtc
      541 gacgcgttcg accagggcgc cgatcgtgcc gagcttgtcc gagaccttgg cgcccccgag
      601 cacggcgacg tagggacgcg ccggatcggc caccagcctc gagagcgcct ccacctcgcg
      661 ctggagcagg cgtcccgcgg cggtctcccc gccgtgctcc cggacgagag ccggaagcgc
      721 gacgaccgat gcatgggcgc ggtgcaccgc gccgaacgcg tcgtcgacgt acaggtcggc
      781 gagcccggcc agccgctgcg cgaacgcacg gtcgttggcc tcctcgcccg ggtcgaaccg
      841 caggttctcg agcaggacga ggcggccggg ttcgatcgtg agggcctgac acgaccgctc
      901 gacttcgggg ctgaggacgc cgtccgtcgg cacgtactcg accacccggt ccaagaggcg
      961 ccggagcagc tcggcgaccg ggcggagtcg gagcgcctcg tccacgcggc ccctcggccg
     1021 gccgaggtgg gaggccgcga cgatcgacgc gccccggtcg agcagctcgg tcagcgtcgg
     1081 caggccggct cggatccgag cgtcgtcggt gaccacaccg tcgtcgagcg ggacgttgag
     1141 gtcgagccgg acgaagacgc gccggcccgc gaggtcacca gcgaggtcgt cgagcgttcg
     1201 caggcgcacc ggcgcctaca acgagcgtgc gatgaggtcg acgaggtcca cgaggcggtt
     1261 cgagtacccc cattcgttgt cgtaccaccc gatgaccttg gccaccctcc ccatcgtcat
     1321 cgtcgaggcc gcgtcgaacg tgcacgacgc cgggcttccc acgatgtcgg aggacacgat
     1381 cggatcctcc gtgtagacga ggcggccgcc cgagagcgga cccctatcgg ccgcggaccg
     1441 gtacgcgtcg ttgatctcgt cgacgctcgc ctcgcgcttc agcgtggcga ccaggtcggt
     1501 gaccgaacca tccgggaccg ggacccgcat cgcgatcccg tccagcttcc cctgcaggtg
     1561 cggcaggacg aggccggtgg ccttcgcggc ccccgtcgac gtggggatga tgttgatcgc
     1621 cgcggcacga gcccggcgca ggtccttgtg cggaaggtcg aggatcgcct ggtcgttcgt
     1681 gtacgcatgg atcgtggtca tgaacgccat ctcgaggccg aacgcgtcgt cgagcacctt
     1741 ggccatcggc gcgacgcagt tggtggtgca ggacgcgttc gagatcacgt tgtgctggga
     1801 cgggtcgtac gcggcatcgt tcacgccgag gacgacggtg aggtcctctc ccttggccgg
     1861 ggcgctgatc acgaccttct ccgcgcccgc ctcgatgtgc ttgttcgccc tctcgcgatc
     1921 ggtgaacagc ccggtcgact cgatcacgat ctgggcgccg agctccttcc acggcagctc
     1981 gaccggatcg cggaccgcca gcacgcgcag ctcgtcgccg tcgaccgtga cgccgccgtc
     2041 gaacgtcccg acctcgccgt cgaaccgccc gagcacggag tcgtacttca gcagatgcgc
     2101 gagcgtcggg gcgtcggtga tgtcgttgac cgcaacgaag tcccagtcca cgccgagctt
     2161 cctcgcggcc cggaagaagt tcctcccaat cctgccgaac ccgttgatgc cgaccttcac
     2221 gctcatggct cctccagggt aggagtccat cctagccaat cgcctgtgtg gccagccggg
     2281 aggcgttcgg gtcgggaagc gtcgtcagcg atcgaggtcg cggtggtcga ccgatgccgg
     2341 caggcccttg tcgaggaaga agctcgcgag gtcctcgacg acgaccactg aacggtgccg
     2401 accgccggtg cagccgatcg cgacggtgag gtaggacttc ccctcggcca cgaagcccgg
     2461 gaccatgaac ccgagcagcg cgcgcagccg gcggaggaac tcccggtacg tggcctgccc
     2521 gcgcacgaat gtccggacgc ggtcgtcggt gccggggagg ggccggagct cctcgaccca
     2581 gtgggggttg ggaaggaacc gacagtcgat cacgaggtcg gcgtcgcgcg gggcgccgta
     2641 cttgtagccg aacgacacca ggctgacctg gagactctcc tcgggaggcg cgtcggagaa
     2701 cgcgtcccgg atgcggtcgc ggagctcgtg gggcgtgagt cccgacgtgt cgatgatcag
     2761 gtccgcgtct ccgcggaggg cctccatcat catccgttcc ttgcggatgc cctcgaccac
     2821 ccggtcggcc ggggcgagcg ggtggcgtcg gcgcgtggat tcgtagcggt tgaccagatc
     2881 gccgtcggac gcctcgagga acaggatccg cgaggggatg ctcagctccg tgagctcctg
     2941 gagcgccttc gagagctcgc cgaagaacac cccgccgcga acgtcgagca cgatcgccac
     3001 ccggcccggt ccgccggggc gcgacccgag ctcggccatc ttcggcaaca gcgcgggcgg
     3061 caggttgtcc accacgaagt acccgaggtc ctcgaggcac cgcgccgcct ccgagcgtcc
     3121 ggcgccggag aggccggtga tgatcgtgaa cccctggttc gcagggccgg ggacggccgc
     3181 ccgcttcgcc gctcgtttcc ggcggtcgga gagcttgctc ggctcctcgg ccgagcccga
     3241 gttcggcacg tcgctcggtt tgcgggcggc gccggtgcgc tccggctggg cggtcacgcg
     3301 ctgcgcctcc ccccggccgg ctcgcggccc gtgagtcgat cgtggatcgc tctggcgaga
     3361 tccgggccga cccccggcgt cgaggcgatc tcgtcctcgc tcgccctcga gatacgtgcc
     3421 accgagccga accgcttcag cagcgccttc ttccgggacg gacccacccc ggccacgtcg
     3481 tcgagcggcg aggccagcgc gcgcttcccc cgcttctcgc ggtggtaggt cacggcgaac
     3541 cggtgagcct cgtcccgcag gtgctgcagc acg
//
LOCUS       DATBRK010000331         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1219180, whole genome shotgun sequence.
ACCESSION   DATBRK010000331 DATBRK010000000
VERSION     DATBRK010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219180"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VIH70_09840"
     CDS             <1..947
                     /locus_tag="VIH70_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8116704.1"
                     /translation="GITAIDTFYGGRERFTAAYLLDADEPAIVETGPSTSFEPVVAGL
                     ERLGIGVADLAHIAVTHIHLDHAGGVGRIAERFPNATVWVHERGARHLADPTRLVESV
                     TSIYGSDTTATLFGPVEPVAAQRIRALGDGDRIELGGRRLEALTTPGHAKHHVALADS
                     STGAVFTGDALGIHPPDAPSLRPATPPPDYDLELALGSIERIRERARGAIVLFSHFGP
                     VHEVERICDLATERFRTWTQAVGEALALGLDPDRDDALDEVMRVLRDVARTDAPTGAE
                     AELDLQAMETMSSIRLNSMGILGYWRRNREAEASGQAS"
     gene            complement(935..1624)
                     /locus_tag="VIH70_09845"
     CDS             complement(935..1624)
                     /locus_tag="VIH70_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8116705.1"
                     /translation="MPKAMRPRSGSKGQPKGRRSGLPPDVVSELRHMSRTGKADMAIA
                     RLERAVALLDRGDARGAAAEAAKAKELAPRAPAVREVLGLALYQLERYPEALSEMQAY
                     RRMSGRADQNHIIADSLRSLGRPDRAVPLAEEALASKGVPLAAKAEAVIVASSALADR
                     GKFDQALGMLRRIRTRDDVAGPEVIRVWYVTGNILEQAGRRADALREFRKILRHDPSA
                     FDAAERAAQLA"
     gene            complement(1702..1818)
                     /gene="rrf"
                     /locus_tag="VIH70_09850"
     rRNA            complement(1702..1818)
                     /gene="rrf"
                     /locus_tag="VIH70_09850"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1907..>1981)
                     /locus_tag="VIH70_09855"
     rRNA            complement(1907..>1981)
                     /locus_tag="VIH70_09855"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          294 a          744 c          658 g          285 t
ORIGIN      
        1 gagggatcac cgcgatcgac accttctacg gcggccgcga acgcttcacc gccgcctatc
       61 tcctggacgc cgacgagccc gcgatcgtcg aaaccggacc gagcacctcc ttcgagcccg
      121 tggtcgcggg gttggaacgg ctcgggatcg gagtcgccga cctcgcgcac atcgccgtca
      181 cacacatcca cctggaccac gccggcgggg tggggcggat cgccgaacgc ttcccgaacg
      241 ccaccgtctg ggtgcacgag cgcggcgctc gacatctcgc cgatcccacc cgcctcgtgg
      301 agagcgtgac gtcgatctac ggctccgaca ccaccgcgac cctgttcggg cccgtcgaac
      361 ccgttgcggc ccaacggatc cgagcgctcg gggacggcga tcgcatcgag ctcggcggcc
      421 ggcgcctcga ggcgctcacc acgccgggcc acgccaagca ccacgtggcg ctcgcggatt
      481 cctcgaccgg cgccgtcttc accggcgacg ccctcggcat ccatccaccc gatgcccctt
      541 cgctccgacc tgccacgccg cccccggact acgacctcga gctcgcgctc ggctcgatcg
      601 agcgcatccg cgaacgggca cgcggcgcga tcgtcctttt ctcgcacttc ggcccggtcc
      661 acgaggtcga gcggatctgc gacctcgcga ccgagcgctt ccgaacgtgg acccaggccg
      721 taggcgaggc gctcgcgctc ggcctcgatc cggaccggga cgacgcgctc gacgaggtca
      781 tgcgcgtact gagggacgtc gctcgcaccg acgcgccgac cggggccgag gccgagctcg
      841 acctgcaggc catggagacg atgtccagca tccgcctgaa ctcgatgggg atcctcgggt
      901 actggcggcg caatcgggag gccgaggcct ccggtcaggc gagctgagcc gcgcgctccg
      961 cggcgtcgaa cgcgctcggg tcgtgccgaa ggatcttgcg gaactcgcgg agggcgtccg
     1021 cacgccggcc ggcctgctcg aggatgttcc ccgtgacgta ccacacgcgg atcacctccg
     1081 gccccgccac gtcgtcgcgc gtccggatcc ggcggagcat cccgagcgcc tggtcgaact
     1141 tgccccggtc cgcgagcgcg ctcgaggcca cgatcaccgc ctctgccttc gcggcgagcg
     1201 gcacaccctt tgacgcgagc gcctcctccg caagggggac ggcgcggtcg ggccggccga
     1261 gcgaacgcag ggagtcggcg atgatgtggt tctggtccgc gcgcccggac atcctccggt
     1321 acgcctgcat ctcggacagc gcctccgggt accgctccag ttggtacagg gcgagcccta
     1381 ggacctcgcg gactgccggc gcccgtgggg cgagctcctt ggccttggcc gcctcggcgg
     1441 cggctcccct ggcgtcgccg cgatcgagga gcgcgacggc gcgttcgagc cgggcgatgg
     1501 ccatgtccgc cttcccggtc cgggacatgt gccgcagctc ggagacgacg tccggcggca
     1561 acccggaacg gcgacccttc ggctgcccct tggaacccga tcgtggacgc atcgctttcg
     1621 gcatgccgag agcgtaccgg gccgcacccc ggtggcctca acgagaagag ccgcccccga
     1681 aggggcggct ctttcgaagg tgtccggcgg cgacctactc tcccacccag ttgcccgggc
     1741 agtaccatcg gcgctggagg gcttaacttc cgggttcggg atgggaccgg gtgtttcccc
     1801 tccgctatga ccaccgaaac ggcgtatccc gatcctctcc atcgggaagc tagcgggaac
     1861 gcgagccccc gagcagagct cgggaactgc gatagcgagc gcgagcaaag tcaagtcctc
     1921 ggcctattag taccggtcgg ctcaacgtgt tgccacgcgt acacctccgg cctatcaacc
     1981 t
//
LOCUS       DATBRK010000332         7550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_780705, whole genome shotgun sequence.
ACCESSION   DATBRK010000332 DATBRK010000000
VERSION     DATBRK010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780705"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VIH70_09860"
     CDS             complement(<1..252)
                     /locus_tag="VIH70_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEY8116706.1"
                     /translation="MATLTCERCGLPLPDEARFCPNCGYPVGAPPPEERKFVTVIFVD
                     LVGSTLLSSQIDPERYREVLTGYYRMVAEELESLRGRAYN"
     gene            327..776
                     /locus_tag="VIH70_09865"
     CDS             327..776
                     /locus_tag="VIH70_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8116707.1"
                     /translation="MTVRPASAQHLRDLAALRRVRDRIDREYAKPLDVEALARGAHMS
                     AGHLSRQFRLAFGESPYSYLMTRRIERAMALLRRGDLSVTEVCFEVGCSSLGTFSTRF
                     TELVGVPPSAYRRQAPREMSGIPPCVAKQVTRPIRNREAPLAEPHVA"
     gene            782..1192
                     /locus_tag="VIH70_09870"
     CDS             782..1192
                     /locus_tag="VIH70_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116708.1"
                     /translation="MDITIYQTLLPHDDPDASLAFYRDTLGFEVRGDVEYGGLHWITV
                     GPPDQRGTSIVLYPPAATPGITDDERRAIAEMMAKGTFASINLATKDVDGVFERLQAG
                     DAEVVQEPTDQPYGVRDCAFRDPAGNLIRIQELR"
     gene            1257..1724
                     /locus_tag="VIH70_09875"
     CDS             1257..1724
                     /locus_tag="VIH70_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8116709.1"
                     /translation="MAEKTTAKRGASTSAKRTGTGSKFTAEERAAMKERARELKAAQN
                     KEEGERALLAKIAEMPEPDRRMAKRLHAIITAAAPDLSPKTWYGMPAYAKDDEIVCFY
                     KSADKFKSRYATFGFEEAANLDEGAMWPTSFALKKLTAADEKKIGALVKKAVS"
     gene            complement(1745..2215)
                     /locus_tag="VIH70_09880"
     CDS             complement(1745..2215)
                     /locus_tag="VIH70_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY8116710.1"
                     /translation="MLERIARPEEIRRWEGDLPVRHRYTPGVAGDAFFTSLKERGVLL
                     GSRCESCAFTYVPARLFCERCFSELSADTEVGPGGELVSFTIVFRGIEGEPLERPQTL
                     GAVRLDGADAVLIHRVESGEDPLEIGERVEVVVEPEPRRTGSILDIAGFRVVET"
     gene            complement(2215..2823)
                     /locus_tag="VIH70_09885"
     CDS             complement(2215..2823)
                     /locus_tag="VIH70_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEY8116711.1"
                     /translation="MGRDGGPPTTATQLETPPAATPATSLSDAEFRSATGAVEDEIGA
                     RYAWDAGVAIGRFLDGLRNGQIMGRECRVCRRVLVPPRMFCERDFRGTDAWVQVENTG
                     VVQTFSICNVRWDMQPLDEPELPAVIAIDGSDGGFLHTLGEVEPEDVTIGMKVEAVWK
                     PVGERTGSILDIAYFRPRRGDPGGSRSERERASDSPPGEGDR"
     gene            complement(2818..3975)
                     /locus_tag="VIH70_09890"
                     /pseudo
     CDS             complement(2818..3975)
                     /locus_tag="VIH70_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014288394.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
     gene            complement(3976..4341)
                     /locus_tag="VIH70_09895"
     CDS             complement(3976..4341)
                     /locus_tag="VIH70_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116712.1"
                     /translation="MRLRALDHVWFWVTDMDRSVRFYEQQLGLGLRVRHGDEWAELEA
                     GAIHIGLHGARSEGNAPHGGTAVFRVDDLDLAKTALEQRGVAFHEHLGEVPGIARYAS
                     FDDPDGNSMQLIEYVPEQG"
     gene            complement(4338..5507)
                     /locus_tag="VIH70_09900"
     CDS             complement(4338..5507)
                     /locus_tag="VIH70_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEY8116713.1"
                     /translation="MEPRVAVVGVGHAGFAPVTAGISYKELMFEAAQRAYHDAGVDPR
                     TEVDSFVCCSEDFLEGTSIFDEYVPDQLGAMQRPVQTVAADGLFGVATGMMLIGSGLA
                     SVVAVEAHSKVSDVRTLGAVEAFALDPVLNRPLRFPTLAVAGLDMNAFLERTRISEEH
                     CAMIAAKNRSNALDNPRAAYPAELAADDVDDSAPVAWPLRALEVAERADGCVVVVLAA
                     EDRARELTEGPVWLLGAGWSSGSPSLESRTWGETEPVTKAAESAYRQAEITDPQEEIG
                     LVEVDDTFAYKELLHLEALGLGEASELAQLLEEGELEPGGDLPVNVSGGSLGQGNLFE
                     ANGLVRLLECVEQLRDEAGERQVDEAYVAVAQSARSAPSTSAAVVVLANDEGAGA"
     gene            complement(5570..5815)
                     /locus_tag="VIH70_09905"
     CDS             complement(5570..5815)
                     /locus_tag="VIH70_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116714.1"
                     /translation="MDTLNRSVARTPPATKIEIVGYVLVAVGYVVMTLGILEIGGLPN
                     IVIGFAIAWAGCVLMFLGEERREFDEEAEFRRELEEL"
     gene            complement(5847..6539)
                     /locus_tag="VIH70_09910"
     CDS             complement(5847..6539)
                     /locus_tag="VIH70_09910"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY8116715.1"
                     /translation="MPFPRKLLIENEQLVLELRPHWIALVMPAIVTILVIAGWIAAVS
                     YGPDDGTGRDVTLWGATAIAIFVLIWFPVRKFVAWVTSYFVVTSDRVIHREGWIAKRS
                     MEIPLEAINDVRFHQGVFERMIGAGDLIISSASEFGREVFGDIRNPEHVQKTIYQQGE
                     LNKDRMYRGVGRDSAHAVPAPPAASGVTTPAMPPASASTTGELERLAELRNKGVLTED
                     EFQSQKKRILGG"
     gene            complement(6549..7301)
                     /locus_tag="VIH70_09915"
     CDS             complement(6549..7301)
                     /locus_tag="VIH70_09915"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY8116716.1"
                     /translation="MLSEDALVRSLERVGLRAPVRFDEVTASTQATALELAAAGTPEW
                     TLVAAGHQTEGRGRLGRTWLDRPGRALMFSFVLRPALDPASGGLLTLLAGTAMAQACF
                     ELADQRTACKWPNDILVAGRKAGGILAESRVEGGRFEHVVVGVGVNLGEPPPDIPEAG
                     AVEAEDDELLEAFVSAFARGYEPAHPAFAGGVVASYREVCATLGTRVRATTGGGTVVE
                     GEAVDLDDRGGLVVRTTAGDEVVRFGEVEHLE"
     gene            7412..>7550
                     /locus_tag="VIH70_09920"
     CDS             7412..>7550
                     /locus_tag="VIH70_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8116717.1"
                     /translation="MAKPRILIANRGEIAVRIIRTVRELGYTSIAVYSDADRDALHVE
                     MA"
BASE COUNT         1176 a         2776 c         2507 g         1091 t
ORIGIN      
        1 gttgtacgcg cggccgcgga gcgactcgag ttcctcggcc accatcctgt agtaaccggt
       61 gaggacctct cggtagcgtt cgggatcgat ctggctggac agcagcgtcg accccacgag
      121 gtcgacgaag atcaccgtca cgaacttgcg ctcctcgggc ggcggggcgc ccaccggata
      181 gccgcagttc gggcagaacc gagcctcgtc cggcagggga agcccgcacc gctcacacgt
      241 cagggtcgcc atgtcgaaag catacgggcg ccggatgacg cgaccttcgc ccggaggcgg
      301 ccggcgcgga cggtaccatc tcgccggtga ccgtcagacc cgcctcggcg cagcacctgc
      361 gcgacctcgc ggctctgcgc cgcgtccgcg accgcatcga ccgcgaatac gccaagccgc
      421 tggacgtcga ggcgctcgcg cgcggcgcgc acatgtcggc cgggcacctc agccgccagt
      481 tccggctcgc gttcggcgaa tccccgtaca gctacctgat gacacggcgc atcgagcgcg
      541 cgatggcgct gctgcgtcgt ggcgatctca gcgtcaccga ggtctgtttc gaggtcggct
      601 gctcgtccct gggaaccttc agcacccgct tcaccgagct ggtgggtgtg ccaccaagcg
      661 cctaccggcg ccaggcgccg cgcgagatgt cggggatacc gccttgtgtg gcgaagcagg
      721 tcaccagacc gatcaggaat cgagaagcgc cgctcgccga gccgcacgta gcatgaccgc
      781 catggacatc accatctacc agacgctcct gccgcacgac gatcctgacg cgtccctggc
      841 cttctaccgc gacacgctcg gcttcgaggt ccggggtgac gtcgaatacg gcgggctgca
      901 ctggatcacg gtcgggcccc ccgaccagcg cggcacgtcc atcgtcctgt acccgcctgc
      961 cgccacaccc ggcatcaccg acgacgagcg ccgcgccatc gccgagatga tggcgaaggg
     1021 aacgttcgcg agcatcaacc tggccaccaa ggacgtcgac ggcgtcttcg aacggctgca
     1081 ggccggcgac gccgaggtcg tccaggagcc taccgaccaa ccgtacgggg ttcgcgactg
     1141 cgccttccgc gatcccgcag gcaacctgat ccgcatccag gagctgcgct gagccgtccg
     1201 gcgatcgcgc cggagacaag cggcacatac gcatcgaagc aaggaggtgg ctcgcaatgg
     1261 ctgagaagac gaccgcgaag aggggcgcca gtacgtccgc caagcgcacc gggacgggca
     1321 gcaagttcac cgccgaggaa cgcgccgcga tgaaggagcg cgcccgggaa ctgaaggcgg
     1381 cccagaacaa ggaggagggg gagcgcgccc tgctcgcaaa gatcgccgag atgccggaac
     1441 cggatcgcag gatggccaag cgactccacg cgatcatcac ggccgccgcg ccggatctct
     1501 cgcccaagac ctggtacggg atgcccgcgt acgccaagga tgacgagatc gtttgcttct
     1561 acaagagcgc ggacaagttc aagtcgaggt acgcgacgtt cggcttcgag gaagcagcga
     1621 acctcgacga gggcgccatg tggccgacct ccttcgccct gaagaagctg acggccgccg
     1681 atgagaagaa gatcggcgcg ctggtgaaga aggcggtgag ctgagcacgg cctcgagatc
     1741 ggtcctaggt ctcgacgacc ctgaagcccg cgatatcgag gatcgatccg gttcgacgtg
     1801 gctccggctc gacgaccacc tcgacgcgtt cgccgatctc cagcggatcc tcgcccgact
     1861 cgacccgatg gatcaggacc gcgtccgcgc catcgagacg gaccgcgccc agggtctggg
     1921 ggcgctcgag cggctcgccc tcgatccccc ggaacacgat cgtgaaggac acgagctcgc
     1981 cgccgggacc gacctcggtg tcggccgaca gctccgagaa gcatcgctcg cagaacagcc
     2041 gcgccggcac gtaggtgaac gcgcagctct cgcaccgcga gccgagcagc acgccgcgct
     2101 ccttgagcga cgtgaagaac gcatcgccgg cgacaccggg cgtgtaccgg tggcggacgg
     2161 gcaggtcccc ctcccagcgt cggatctctt cgggacgcgc gatcctctcg agcatcagcg
     2221 gtcgccctct cccggggggc tatcgctcgc gcgttcgcgc tcgctccttg agccccccgg
     2281 atccccccgc cgtggtctga agtaggcgat gtcgaggatc gagccggtgc gttcgcccac
     2341 cggcttccac acggcctcca ccttcatccc gatcgtgaca tcttccggct cgacctcgcc
     2401 gagcgtgtgc aggaacccgc cgtccgatcc gtcgatcgcg atcacggcgg ggagctcggg
     2461 ctcgtcgagg ggctgcatat cccaccgcac gttgcagatc gagaacgtct gcacgacgcc
     2521 ggtgttctcg acctgcaccc aggcgtcggt gccacggaag tcgcgctcgc agaacatccg
     2581 aggtggcacc agcacccgac ggcacacgcg gcactcgcga cccatgatct gtccgttgcg
     2641 gagcccgtcg aggaaccggc cgatcgcgac gcccgcatcc caggcatagc gtgcgccgat
     2701 ctcgtcctcg acggcgccgg tggccgaacg gaactcggcg tccgagagcg acgtcgccgg
     2761 cgtcgcggcg ggcggcgtct cgagctgcgt cgccgtcgtc ggcgggccgc catcacgacc
     2821 catcaccacg accgtcccga cctgcatgag gtcgccccat gcctgggcga cgcctcgttc
     2881 gggcgagccc gggacctggc ggccgcccgc ctcgcctcga agctgccaga agagctcggc
     2941 gatcttcatg aggccggccg ccgcgatggg gttgccgacg ccgaggaggc cgccgctcgg
     3001 gcacgagggc aggtcgccct tgcggtcgag gacgccgtct cgggacgcct cgggcgcctt
     3061 acctctgtcg aacagcagca ggccctcgag gtggtgcagc tccttgtagt cgaaggggtc
     3121 gtagggctcg gcgacgtgga tctgcttgcg cggctcggtc acgccggcca tcccgtaggc
     3181 catgcgggcc gcgttcgcga cgtagtccgg gaacacgagg tcgcgattgg tccagtacgc
     3241 ggtgtcgagg ttccacccga ccccctggat ccagaccggg tcgctcgtga tctgcttcgc
     3301 ggcctcctcg ctcgccacga cgatcgcgac ggcgccgtcg ctgatcggcg agacgtccag
     3361 gcgctgcacc ggccatgcga gcgtttcgga ggcgagcacg tcttcgatcg tgatgtcggc
     3421 ctggccgagg agcgccgccg ggtggtcggc cgcgtttcgc ttgttcttca ccgcgacggc
     3481 tgcgatgtcc cgcttgtcga tgccgtgcac ggtcatgtag cggttcatct cgagcgcgaa
     3541 gatccacagg aggttcgggc cgagcgggcg ctcgaggatg ttgtcgaaga tcgtgaggaa
     3601 cgcgccctgg ggatgcggtt gcgtcgaaga catcttctcc tcggccacgg cgagcacgac
     3661 atcggccatc ccgctcgcga ccgtgtagaa cgcctggatc gccgtgaaca cgccgctgcc
     3721 cccgcccacg tacgtccgga tgaagggccg gccgaacgca cccgccccgt ccgacaggta
     3781 ctcccccttc atgtgcacgc cgtcgaaggc gtcgggggcg ctggtcaagc acaccgcgtc
     3841 gatccgatcg agcgggagct cgcaggacgc gagcgcctcg cgcgaggcct cccacgcgag
     3901 ctccttgccg gtctcctgcg cgcgacgaac gaatctcgtc atccccgcgc ccacgaccgc
     3961 gacccgccgg gccatctagc cctgctccgg gacgtactcg atcagctgca tcgaattgcc
     4021 gtcggggtcg tcgaacgatg cgtatcgcgc gatgcccggc acctcgccga ggtgctcgtg
     4081 gaacgccacg ccgcgctgct cgagcgcggt cttcgcgagg tcgaggtcgt ccacgcggaa
     4141 cacggcggtg ccaccgtgcg gcgcgttgcc ctcgctgcgt gcgccgtgga gcccgatgtg
     4201 gatcgcgccg gcctcgagct cggcccattc gtcgccgtgc cggacccgca gcccgagccc
     4261 gagctgctgc tcgtagaagc gcaccgaccg atccatgtcg gtcacccaga accaaacgtg
     4321 gtcgagggcc cggagcctca tgcacctgct ccctcgtcgt tcgcgaggac gacgacggcg
     4381 gccgacgtgg acggcgcgct gcgcgcggac tgcgcgaccg ccacgtaggc ctcgtcgacc
     4441 tggcgctcgc cggcctcgtc acggagctgc tcgacgcact ccagcagccg gacgaggccg
     4501 ttcgcctcga acaggttccc ctgcccgagc gagccgcccg agacgttcac cgggaggtcc
     4561 ccacccggct cgagctcgcc ctcctcgagc agctgcgcga gctccgacgc ctcgcccagc
     4621 ccgagcgcct ccaggtgcag cagctccttg tacgcgaagg tgtcgtccac ctcgaccagg
     4681 ccgatctctt cctgcgggtc ggtgatctcg gcctggcggt acgcggactc ggccgccttg
     4741 gtcacgggtt cggtctcgcc ccacgtccgg ctctcgagcg acgggctccc ggacgaccag
     4801 cccgcgccga gcagccacac cggcccttcg gtgagctcgc gcgcccggtc ctccgcggcg
     4861 aggacgacca cgacgcagcc gtcggcgcgt tcggccacct cgagcgccct gagcggccac
     4921 gcgaccggcg ccgagtcgtc cacgtcgtcg gccgcgagct cggccgggta cgcggcgcgc
     4981 gggttgtcga gcgcgttcga gcggttcttg gccgcgatca tggcgcagtg ctcctcggag
     5041 atgcgggtgc gttcgaggaa cgcgttcatg tccaggccgg cgaccgcgag cgtcgggaag
     5101 cgcagcggtc ggttgagcac cgggtcgagg gcgaacgcct cgaccgcgcc gagcgtccgg
     5161 acgtccgaga ccttgctgtg cgcctcgacg gccacgaccg acgcgaggcc cgagccgatc
     5221 agcatcattc ccgtcgccac gccgaacagc ccgtccgccg cgaccgtctg cacgggccgc
     5281 tgcatcgcgc cgagctggtc gggcacgtac tcgtcgaaga tcgatgtgcc ttcgaggaag
     5341 tcctccgagc agcagacgaa cgagtcgacc tcggtccgcg ggtccacgcc ggcgtcgtgg
     5401 taggcccgct gcgccgcctc gaacatcagc tccttgtacg agatccctgc ggtgaccggc
     5461 gcgaaccccg cgtggcccac gccgacgacg gcgaccctcg gctccacgtg ggcgattgtc
     5521 ccatcgagcc gcggaccccg ctcgccgagg gcgcgccggg ccgcgagcct cagagctctt
     5581 cgagctccct gcggaactcg gcctcctcgt cgaactcgcg acgctcctcc ccgaggaaca
     5641 tcagcacgca gcccgcccac gcgatcgcga aaccgatcac gatgttgggc aacccgccga
     5701 tctcgaggat ccccagcgtc atcacgacgt agccgacggc caccaggacg tacccgacga
     5761 tctcgatctt cgtcgccggt ggtgtccgcg ctaccgatcg gttcagggtg tccatgccgt
     5821 ctcgctgtcg accggatcca cggagtctac cccccgagga tcctcttctt ctggctctgg
     5881 aactcgtcct ccgtgagcac gcccttgttc cgcagctcgg ccaggcgctc gagctcgccc
     5941 gtcgtggagg cggaggccgg cggcatggcg ggcgtcgtca cacccgatgc agccgggggg
     6001 gccggcaccg cgtgtgccga gtcgcgcccg acccctcggt acatccggtc cttgttgagc
     6061 tcgccctgct ggtagatcgt cttctggacg tgctcggggt tgcggatgtc gccgaacacc
     6121 tcgcgcccga actcgctcgc cgagctgatg atcaggtcgc cggccccgat catccgctcg
     6181 aacaccccct ggtggaaccg cacgtcgttg atcgcctcca gcgggatctc catcgagcgc
     6241 ttcgcgatcc acccctcgcg atggatgacc cggtccgacg tcacgacgaa atacgacgtc
     6301 acccacgcga cgaacttgcg caccgggaac cagatcagca cgaagatcgc gatcgcggtc
     6361 gcgccccaga gcgtgacgtc gcgcccggtg ccgtcatcgg ggccgtacga caccgccgcg
     6421 atccaccccg cgatcacgag gatcgtgacg atcgccggca tcacgagggc gatccagtgc
     6481 ggccgcagct cgaggaccag ctgctcgttc tcgatcagca gcttgcgggg gaacggcatg
     6541 agcgcatcct actccaggtg ctcgacctcc ccgaaccgca cgacctcgtc gcccgccgtc
     6601 gttcgcacga ccagtccgcc gcgatcgtcc aggtccacgg cctctccctc gacgaccgtg
     6661 ccgccccccg tcgtggcccg gacgcgggtg ccgagcgtcg cgcacacctc ccggtaggac
     6721 gcgaccacgc cccccgcgaa cgcgggatgc gcgggctcgt acccgcgcgc gaacgcggac
     6781 acgaacgcct cgagcagctc gtcgtcctcg gcctcgacgg cgccggcctc ggggatgtcc
     6841 ggcggcggct cccccaggtt cacgccgacg ccgaccacga cgtgctcgaa ccggccgccc
     6901 tcgacacggg actcggccag gatcccgccc gccttccgcc cggctaccag gatgtcgttc
     6961 ggccacttgc aggcggtccg ttggtcggcc agctcgaagc aggcctgcgc catcgccgtg
     7021 ccggccagca acgtgagcag cccgccggac gcggggtcga gggcgggtcg cagcacgaac
     7081 gagaacatca gcgcccggcc cggccggtcg agccacgtgc gcccaaggcg gccgcggccc
     7141 tcggtctgat ggcccgcggc gacgagggtc cactcgggcg tgcctgcggc cgccagctcg
     7201 agggccgtcg cctgggtcga ggcagtaacc tcgtcgaacc gcacgggtgc acgcagaccc
     7261 acacgttcga gcgaccggac cagggcgtcc tcggacagca ccagacgttt gactcccgtt
     7321 cgggggtacc cgattgttcc gtgggtaccg ggttccgcgg accctcggga ggccgacggt
     7381 ccgatcgagc aagcatgacg gagcggagcg cgtggcgaaa cctcggatcc tgatcgcgaa
     7441 tcgcggcgag atcgcggtcc gcatcatccg gacggtccga gagctcggct atacgtcgat
     7501 cgccgtctac agcgacgccg accgcgacgc cctgcatgtc gagatggccg
//
LOCUS       DATBRK010000333         4247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1935761, whole genome shotgun sequence.
ACCESSION   DATBRK010000333 DATBRK010000000
VERSION     DATBRK010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935761"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="VIH70_09925"
     CDS             complement(<1..1613)
                     /locus_tag="VIH70_09925"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEY8116718.1"
                     /translation="MTSPVGIRGDRPGVAPRDTRDDLVAILVLAVLYYAAARLGLRLA
                     IVEDNITPLWPPTGLALVAFLRYGRRMWPGVAIAAFMVNLPITNFPAAVLTTAGNTLA
                     PIVAAWLLERAGFRQQIDRVRDVVALVGLGALLSMTISATIGSAALVLSSRIALGGFW
                     GAWSVWWAGDAMGILVVAPFLLCVLTWNQVEPRLGLRRLPEALVMLTLLLGASVFALW
                     NTGQLLFLLPPLVGWIAWRFQLRGAAPAVLLVSTVAAWAASRMVGWFADLSITEEMLT
                     LQLFNATIAFTAFFLSALVSERTQARERLQRAAEDLEGRVRRRTSQLVDANEHLGQQI
                     TERREAETKLLRSERELAEAQELARLGRWEWDLGSGTVSWSDDLYRIHGYEPGAFPMT
                     FEKAVELVPEEDRRRVEHNLTAALTMRRREVPDIEYRIVRPDGELRALLGRARVFFDE
                     AGSPIRMLGVVQDVTERVDYDRQHLIADTLQRALLPQDLPTVDGLELAARYQPAEVGF
                     KAGGDWYDVIPLVGGRVGLVIGDVAGHGL"
     gene            complement(1679..3688)
                     /locus_tag="VIH70_09930"
     CDS             complement(1679..3688)
                     /locus_tag="VIH70_09930"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HEY8116719.1"
                     /translation="MKRSLASLVPLVVLLGLSVPPVQAAQPGQFVLRCGYSHTLPDDP
                     IVFPGQPGASHLHDFYGNTGVNAFSTFETMLAGETNCRVPSDTAGYWAPTGFLNGVRL
                     RPGVMRIYYLAPATGTPGTIPAGLQMVGGNKDAQSPAENPHVSWSCGQTTSVSTPHRD
                     TPYDCRPWAQYGFVDGIVASIDFPSCWNGTGLRPEDVAYPEGGACPPGFGNVIPRLSE
                     RVHYGVMNPLGIDGTLAFQLSSGPPYSMHADFWNTWQQERLDQLVADCLVANVHCGSV
                     DAANTIRWVRQFGTQRYDLANAAAPDGKGGSYTAGFTNFSLDGKPYHHRFDAFLTRYD
                     AEGDKLWTRQFGTNGTDQARAISVSGTDVYVVGSTDGRFPKQTARGGTDAFVARFNAR
                     GRLTWLKQFGTHRDDEGAAVSAGTDAIFLAGTTRGPLDQQRLDGPSDAFVMRLDTHGF
                     PSWARVLGGDGEDLGLSVAQRAGQVFLAGTTEGLLHTVADQDGFIAGFDRRGRPAWSY
                     PLGASDTDAITSIVPRAEGVYFAGWTSGTFLDEPPAGGLDAVIGKLRVNGGLSWLKQF
                     GSTTDDQATALSTTGKGLYVAGSTIGELPDGTPLGESDGFLRKYLPNGTEVWTRQFGT
                     ADFDAVYGMAADPRGVVAVGTTHGAFEGQTNAGDRDAFLVRIAFS"
     gene            3918..>4247
                     /locus_tag="VIH70_09935"
     CDS             3918..>4247
                     /locus_tag="VIH70_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY8116720.1"
                     /translation="MRTLIKGGTVVTATETTKADVVIDDERVAAIGVGMPVQADVEID
                     ATDRYVMPGGVDVHTHMDLPFGGSFCSDDFETGTRAAAFGGTTTIVDFALQDYGEGLR
                     QGLDRWHE"
BASE COUNT          686 a         1511 c         1476 g          574 t
ORIGIN      
        1 tcgagcccgt gccccgcgac atcgccgatc acgagcccga cgcggccgcc gaccagcggg
       61 atcacgtcgt accagtcgcc gcccgccttg aacccgacct cggccggctg gtaccgcgcg
      121 gcgagttcga gcccgtcgac cgtcgggagg tcctggggca gcagcgcgcg ctggagcgtg
      181 tcggcgatca ggtgctggcg gtcgtagtcc acgcgttcgg tgacgtcctg gacgaccccg
      241 agcatccgaa tgggggagcc ggcctcgtcg aagaacacgc gggcgcgtcc gaggagcgct
      301 cggagctcgc cgtccgggcg gacgatccgg tactcgatgt cgggcacctc tcgccgccgc
      361 atcgtcagcg cggccgtaag gttgtgttcg acccgccggc ggtcctcctc gggcacgagc
      421 tccacggcct tctcgaacgt catggggaac gctcccggct cgtagccgtg gatccggtac
      481 agatcatcgg accagctgac cgtccccgac ccaaggtccc actcccaccg accgagcctc
      541 gctagttcct gcgcctccgc gagctcgcgt tcgctccgaa ggagcttggt ctcggcctcg
      601 cgccgttcgg tgatctgctg cccgaggtgc tcgttggcat cgaccagctg cgacgtgcgc
      661 cggcggacgc ggccctcgag gtcctcggcg gcgcgctgca gccgctcgcg ggcctgcgtg
      721 cgttcggaga cgagcgccga caggaagaac gcggtgaacg cgatcgtggc attgaacagc
      781 tgcagcgtga gcatctcctc ggtgatcgac aggtcggcga accatccgac catccggctg
      841 gcggcccacg cggccacggt cgacacgagg agcaccgccg gggccgcgcc gcggagctgg
      901 aaacgccacg cgatccaccc gacgagcggg ggaagcagga acaggagctg acccgtgttc
      961 cacagcgcga acacggaggc gcccagcagg agcgtcagca tgaccagcgc ctcggggagg
     1021 cggcggaggc cgagcctggg ttcgacctgg ttccaggtga gcacgcacag caggaacggc
     1081 gccaccacga ggatccccat cgcgtcgccg gcccaccaca cggaccacgc accccagaag
     1141 ccgccgagcg cgatgcgcga cgacagcacc agcgcggcgc tcccgatcgt cgcactgatc
     1201 gtcatgctca gcagcgcgcc gagccccacg agcgccacga cgtcgcggac gcgatcgatc
     1261 tgctgccgga agccggcccg ttcgagcagc caggccgcca cgatcggcgc gagggtgttc
     1321 ccggcggtgg tgaggacggc cgcagggaag ttcgtgatcg gcaggttgac catgaacgcc
     1381 gcgatcgcaa ccccgggcca catacgccgg ccgtaccgca ggaacgccac caacgcgaga
     1441 ccggtcggcg gccagagcgg cgtgatgttg tcctcgacga tcgcgagtcg gagtccgagc
     1501 cgggcggcgg cgtagtacag gaccgcgagc acgaggatcg cgacgaggtc atcgcgggtg
     1561 tcccgcggcg cgacgccggg tcgatcccca cggatcccga ccggggaggt catcaggcgg
     1621 ccagcttcca ccagatcacc cagcgtgcca atcccccgga gcgggggcgg ccgtgccctc
     1681 aggagaacgc gatcctgacg aggaaggcgt cccggtcgcc ggcgttcgtc tggccctcga
     1741 acgccccgtg cgtggtcccg acggcgacca cgcccctcgg gtccgcagcc atcccgtaga
     1801 ccgcgtcgaa gtccgccgtg ccgaactgcc gcgtccagac ctcggtgccg ttcggcaggt
     1861 acttgcgcag gaagccgtcg ctctcgccca gcggtgtgcc gtcggggagc tcgccgatcg
     1921 tggagccggc gacgtacaga cccttgcccg tcgtcgagag cgccgtcgcc tggtcgtcgg
     1981 tcgtgctccc gaactgcttg agccacgaca agcccccgtt cactcggagc ttgccgatca
     2041 ccgcgtccaa cccgcctgcc ggcggctcgt ccaggaaggt ccccgaggtc cacccagcga
     2101 aatacacgcc ctccgcgcgc gggacgatcg acgtgatcgc gtcggtgtcc gacgcgccga
     2161 gcgggtagct ccacgccggc cgaccgcgcc gatcgaaccc cgcgatgaac ccgtcctggt
     2221 ccgccaccgt gtgaagcagg ccctccgtcg tccccgcgag gaacacctgg cccgcgcgct
     2281 gcgcgaccga caggcccagg tcctcaccgt ccccgccgag gaccctcgcc cagctgggaa
     2341 aaccgtgggt gtcgagccgc atcacgaacg cgtcggacgg cccgtcgagc cgctgctgat
     2401 cgagcggccc gcgggtcgtg ccggcgagga agatcgcgtc cgtgccggcc gagaccgcgg
     2461 cgccctcgtc gtcgcggtgc gtgccgaact gcttcagcca cgtcagcctg ccgcgggcgt
     2521 tgaaccgggc aacgaacgcg tcggtccccc ctctcgccgt ttgcttgggg aaccgcccgt
     2581 cggtcgaacc cacgacgtac acgtcggtcc ccgagacgga gatcgcacga gcctgatcgg
     2641 tcccgttcgt gccgaactgc cgggtccaca gcttgtcgcc ctccgcgtcg taccgggtga
     2701 ggaacgcgtc gaatcgatgg tggtacggct ttccgtcgag cgagaagttc gtgaacccgg
     2761 cggtgtacga gccgcccttg ccgtccggcg cggcggcgtt cgcgaggtcg taccgctgcg
     2821 tcccgaactg ccgcacccac cggatcgtgt tcgcggcgtc caccgagccg cagtggacgt
     2881 tcgcgaccag gcagtccgcg acgagctgat cgagacgctc ctgctgccag gtgttccaga
     2941 agtccgcgtg catcgagtac ggggggccgc tcgagagctg gaacgcgagc gtcccgtcga
     3001 tcccgagcgg gttcatcacg ccgtaatgca cgcgttcgct cagcctgggg atgacgttcc
     3061 cgaaccccgg cgggcacgcg ccgccctccg ggtacgcgac gtcctccggc cggaggccgg
     3121 tgccgttcca gcagctcggg aagtcgatcg acgcgacgat cccgtcgacg aacccgtact
     3181 gggcccacgg cctgcagtcg tacggcgtgt cccggtgggg agtggacacg ctcgtggtct
     3241 gtccgcagct ccacgacacg tgcgggttct cggcgggcga ttgggcatct ttgttgccgc
     3301 ccaccatctg cagtcccgcc gggatcgtcc cgggcgtccc tgtggcggga gccaggtagt
     3361 agatgcgcat gacgccgggc cggagccgca cgccgttcag gaacccggtg ggggcccagt
     3421 agccggccgt gtcgctcggg acgcggcagt tggtctcgcc ggcgagcatc gtctcgaagg
     3481 tcgagaacgc gttcacgccc gtgttgccgt agaagtcgtg gaggtgggat gcgcccggtt
     3541 ggcccggaaa cacgatcggg tcgtccggca gcgtatgcga gtacccgcac cgcaacacga
     3601 actgaccggg ctgcgccgcc tgcaccgggg gaacggacag accgagcagg accacgagag
     3661 ggacgagcga tgccagcgag cgcttcatgg aaggtccgaa cgctacccgg gcgtccgagg
     3721 acgggcaagg gccctcccac cgccggcggt ttgcccgccg cggagcaccg tcggatgatg
     3781 cctggagcgc acgacggcca cgcaacacgg ccaaagaacc gggccaaaga accgggccga
     3841 agaaccgggc caaagaaccg ggccaaagaa ccgggccaaa gaaccgggcc aaagaaccgg
     3901 gaaggagatc gaacgccatg aggaccctca tcaagggtgg cacggtcgtg accgccaccg
     3961 agaccacgaa ggccgacgtc gtgatcgacg acgagcgggt tgccgcgatc ggcgtcggga
     4021 tgccggtcca ggccgatgtc gagatcgatg ccaccgatcg gtacgtgatg ccgggcggcg
     4081 tcgacgtcca tacccatatg gaccttccgt tcggcggcag cttctgttcg gacgacttcg
     4141 agaccgggac gcgggcggcc gcgttcggcg ggaccaccac gatcgtggac ttcgcgctcc
     4201 aggactacgg ggagggcctc cggcagggtc tcgaccgctg gcacgag
//
LOCUS       DATBRK010000334         4790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1071952, whole genome shotgun sequence.
ACCESSION   DATBRK010000334 DATBRK010000000
VERSION     DATBRK010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071952"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VIH70_09940"
     CDS             complement(<1..260)
                     /locus_tag="VIH70_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8116721.1"
                     /translation="MTGLLRTVRFLERHELRPGYDVVIVGGGVNGLSLAYHLAEVHGI
                     RDVAVLERSYLGSGASGRNTQVLRANYNTPETVPLYEASLRM"
     gene            complement(257..679)
                     /locus_tag="VIH70_09945"
     CDS             complement(257..679)
                     /locus_tag="VIH70_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116722.1"
                     /translation="MAELRTWAPTVTSVLASAEACDRVGDLPGAVRVSPCEVALVGDV
                     SEAAVAAAVVRLDADAIVLDVSDGWGAHSLDGPGSREAFARLSELELPVSGSLVGGVA
                     GVGVRILVDRDRIDLLVPSMVLAHVRERIETDCAELLR"
     gene            complement(672..3242)
                     /locus_tag="VIH70_09950"
     CDS             complement(672..3242)
                     /locus_tag="VIH70_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8116723.1"
                     /translation="MTPGPLRFEGREVPVLDGDTVASALFRAGVRTFSRSLKGHRRRG
                     LYCGTGECPNCTITVDGVPGVRSCITPAAGGMRLERGRGWPSAEHDLLHVADLAHALM
                     PVGFYLKAFIRPRFAWRMAERVIRRATGSGPLPLDAPAARAVARYARCDTIVVGAGIA
                     GLTAAIEAAAGGEGVLLCDEFAIGSRLAPGPTLIAVRALEARARATPAIEILERHAAL
                     GLYDGPTVPLATSDELVQVRADRVVVATGAAESHQLFPGNDLPGVWLGRAASAMAAVH
                     GVPVGRRVVVVASTTEGVEHLRALVAAGAGVVAALVPAAFVDEVPHEAEAIVDGELVE
                     ARGHGRLRSVIVRELGERRRIACDALVLSTGLQPRDELARMAVGEPVRLAGDAALDAD
                     EPGLGGGYLCLCEDVTLHDAEQAWAEGFRSAEILKRYTTATMGPCRGAMCGHALACFA
                     RSRPEAADPRSGARTTARPPLRPVALQTLAAPVHEVIERRTGLHDTHVGGGAALGWSG
                     GWKRPISYGDPAEEYRAVRERVGLMDVGTLGRFLIAGRDATTLAGAVFAGRVEDLAPG
                     RSRYVLALDEGGYVVDDGLLCALEDGSYLLTSTSGGAERMDARLREWAERLGLHVHVL
                     ERTSELGAILVAGPRARDLVGALTLDPIDAETLAYPGHRELTVAGVPCRAIRSGFVGE
                     LAFELHHPRSNGPKLWTSLHRAGRDLGVSPFGLEALELLRLEKGHVYLGQDTLPDDTP
                     AKLGLARAVDMTKPWFVGKAALERLAELPPTRRLAGLVLEGAADDARLRGAPLTVGAA
                     VVGRVTSAARSPVLDRAIGLGWIRRGRDGFPDELRAGNAVARVVSTPFYDREGTRVRG
                     "
     gene            complement(3239..3466)
                     /locus_tag="VIH70_09955"
     CDS             complement(3239..3466)
                     /locus_tag="VIH70_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY8116724.1"
                     /translation="MTLEIACPNCGARPHTEFAFGGEDRPHAAPDPAAEFERVFYPDN
                     LEGTQRERWYHALGCRSWFVVTRDTRTDRIG"
     gene            complement(3463..>4790)
                     /locus_tag="VIH70_09960"
     CDS             complement(3463..>4790)
                     /locus_tag="VIH70_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8116725.1"
                     /translation="EDARVLPWWSTHERTAEEIRRISARDADAFARVTERLHELARSL
                     QPFFLEEPPNLYATGWSKLREGRRLFRRFRHLSGDQLSDLVRFATGSLGEFVERHFES
                     DEVRRLYLANNVYGMHAPPYRPGTAIGLLFHMLSGGEHHIQGFNGHVIGGMGSITQAM
                     ASAVRDAGGEIRTGSEVARIDVRDGRATGVTLEDGTEIPARTVLSNADPKRTFLGLVE
                     KSQLSEDFRADVEAIAMAGPCAKVNFALVEEPRWNGMPEDAGPNQRSLATLVPTLEGA
                     QRMYDRHRWGEIPDELWVDCVTSSNVDDTLAPPGTHVMTAFVQYVPFALRSGTWDERR
                     DELGRKVVETISRHSDNVASSIRAMQIVTPLDLARTYGLTEGNIFHGDLNVEQLFFMR
                     PLPAWSRYRTPVDGLYLCGAGTHPGGGVTGAPGYVSSRAVLRDLKRRRP"
BASE COUNT          684 a         1803 c         1713 g          590 t
ORIGIN      
        1 tacatccgca ggctcgcctc gtacaggggg acggtctcgg gcgtgttgta gttggctcgg
       61 agcacctgcg tgttgcgccc cgatgctccc gagccgaggt aggagcgttc gaggacggcg
      121 acgtcgcgga tgccgtggac ctccgcgagg tggtacgcga gggagaggcc gttcacgccg
      181 cccccgacga tcaccacgtc gtagccgggc cggagctcgt gccgctcgag gaaccggacc
      241 gtccgcagca gaccggtcat cgcagcagct ccgcgcagtc ggtctcgatg cgctcgcgca
      301 cgtgcgccaa caccatcgag ggcacgagca gatcgatccg gtcccggtcg acgaggatcc
      361 gcacgcccac gccggcaacc cccccgacga gggagcccga gaccgggagc tcgagctcgg
      421 acaggcgcgc gaaggcctcg cgcgaccccg gcccgtcgag cgagtgcgcg ccccagccgt
      481 ccgagacgtc gagcacgatc gcgtccgcgt ccaggcgcac gaccgccgcc gcaacggccg
      541 cctccgagac gtcgccgacc agcgcgacct cgcagggcga gacgcggacg gcgccgggga
      601 ggtcgcccac ccggtcgcac gcctcggccg aggcgagcac gctcgtcacc gtcggcgccc
      661 aggtgcggag ctcagccacg gaccctcgtc ccctcgcggt cgtagaacgg ggtcgacacg
      721 acccgggcga ccgcgttccc cgctcggagc tcgtcgggga acccgtcccg gcctcgccgg
      781 atccacccga gtccgatcgc tcgatccagc acgggcgaac gtgcggccga cgtgacgcga
      841 ccgacgacgg cggcgccgac cgtgagcggc gcgccccgga gccgggcgtc gtccgccgcg
      901 ccctccaaga ccagcccggc gagccttcgg gtgggcggca gctcggccag gcgttcgagc
      961 gcggccttgc cgacgaacca tggcttggtc atgtcgacgg cccgggcgag cccgagcttg
     1021 gccggcgtgt cgtcgggcag ggtgtcctga ccgagataga cgtgtccctt ctccagacgg
     1081 agcagctcga gagcctcgag cccgaacggc gagaccccca ggtcacgtcc cgcccggtgc
     1141 agcgaggtcc acagcttcgg gccgttcgag cgcgggtggt gcagctcgaa ggccagctcg
     1201 ccgacgaagc cgctccggat cgcccggcac gggacgcccg cgaccgtcag ctcgcgatgg
     1261 cccgggtacg caagcgtctc ggcgtcgatc ggatccaggg tcagggcgcc caccaggtcg
     1321 cgcgcgcggg gaccggccac caggatcgcc ccgagctccg aggtccgctc gagcacgtgg
     1381 acgtgcagtc cgagccgttc ggcccactcg cgaaggcggg cgtccatccg ttcggcgccc
     1441 ccggaggtcg acgtgagcag gtacgagccg tcctccagcg cgcagagcag cccgtcgtcg
     1501 accacgtacc cgccctcgtc gagcgcgagc acgtaacgcg agcgccccgg cgcgagatcc
     1561 tcgacccttc ccgcgaagac ggcgccggcg agcgtcgtcg cgtcgcgccc ggcgatcagg
     1621 aaccggccca gcgtcccgac gtccatcagg ccgacccgct cgcgcaccgc gcggtactcc
     1681 tcggccgggt ccccgtagga gatcgggcgc ttccacccgc ccgaccatcc gagcgcggcg
     1741 ccgcccccga cgtgggtgtc gtgcagaccg gtccgcctct cgatcacctc gtggaccggc
     1801 gccgccagcg tctggagggc gaccggccga aggggtggcc gggcggtggt ccgcgcgccc
     1861 gaacgcgggt cggccgcttc gggtcggctg cgggcgaagc acgccagcgc gtggccgcac
     1921 atcgcgcccc tgcacggccc catcgtcgcg gtcgtgtagc gcttgaggat ctcggccgag
     1981 cggaagccct cggcccacgc ctgctcggcg tcgtgcagcg tgacgtcctc gcacaggcac
     2041 aggtagccgc caccgagccc gggttcgtcg gcgtccagcg ccgcgtcccc ggcgagccgc
     2101 accggttcgc ccaccgccat ccgagcgagc tcgtcgcgcg gttgcagacc cgtcgagagc
     2161 acgagcgcgt cgcacgcgat ccggcgccgt tcgccgagct cgcggacgat caccgagcgc
     2221 agtctgccgt ggccgcgcgc ctcgacgagc tcgccgtcca cgatcgcctc ggcctcgtgc
     2281 ggcacctcgt ccacgaaggc cgccggcacg agcgcggcga cgacccctgc ccccgccgcg
     2341 acgagcgccc gcagatgctc gacgccctcg gtcgtcgacg cgacgacgac cacgcgccgg
     2401 ccaacaggga cgccgtgcac cgccgccatc gccgaggctg cgcgaccgag ccacactccc
     2461 gggaggtcgt tgccggggaa gagctggtgg gactcggcgg ccccggtcgc gaccaccaca
     2521 cggtcggcgc gcacctgcac gagctcgtcc gaggtcgcga gggggacggt cggaccgtcg
     2581 tacaggccga gcgcggcatg ccgctcgagg atctcgatcg ccggggtcgc ccgggctcgg
     2641 gcctcgagcg cccggaccgc gatgagcgtc ggacccggcg cgagccggga gccgatcgcg
     2701 aactcatcgc acaggaggac gccctcgccg cccgcggcgg cctcgatcgc cgcggtcagg
     2761 ccggcgatgc cggcgccgac cacgatcgtg tcgcaccggg cgtaccgggc gaccgcgcgt
     2821 gcggcgggcg catcgagggg cagcggcccc gaacccgtcg cgcgccggat cacgcgttcg
     2881 gccatccgcc acgcgaaccg gggtcggatg aacgccttca ggtagaaccc gaccggcatc
     2941 agcgcgtgcg cgagatccgc gacgtggagg aggtcgtgtt cggcggaggg ccagcccctg
     3001 ccgcgttcga ggcgcatgcc gccggcggcg ggggtgatgc acgaccgcac gcccgggacg
     3061 ccatcgaccg tgatggtgca gttcgggcac tcgccggtcc cgcagtacag tccgcggcgg
     3121 cgatgtccct tcagcgatcg cgagaacgtt cgcactcccg cacggaacag ggccgaggcg
     3181 accgtgtcgc cgtcgagcac cgggacctcg cgcccctcga accgcaacgg gcccggcgtc
     3241 atccgatccg gtcggtccgg gtgtcgcggg tgacgacgaa ccagctccgg cagccgagcg
     3301 cgtggtacca gcgctcgcgc tgcgtaccct caaggttgtc cgggtagaag acccgctcga
     3361 actcggccgc ggggtcgggc gcggcatggg ggcgatcctc gccgccgaac gcgaactccg
     3421 tgtgcggccg ggcgccgcag ttcgggcagg cgatctcgag cgtcacggcc gccgccgctt
     3481 gaggtccctc aacaccgcgc gcgaggagac gtagccgggc gccccggtca cgccgccacc
     3541 cgggtgcgtg ccggccccgc acaggtacag cccgtcgacc ggcgtccggt accgcgacca
     3601 cgctggcagc gggcgcatga agaagagctg ctccacgttc aggtcgccgt ggaagatgtt
     3661 gccctcggtg aggccgtacg tgcgtgcgag gtcgagcggg gtgacgatct gcatcgcgcg
     3721 gatcgacgac gccacgttgt cggagtgccg ggagatcgtc tcgacgacct tgcgaccgag
     3781 ctcgtcccgc cgttcgtccc aggtgcccga ccgcagcgcg aacgggacgt actgcacgaa
     3841 cgccgtcatc acgtgcgtgc cgggcggcgc gagcgtgtcg tccacgttcg aggaggtgac
     3901 gcagtccacc cagagctcgt ctgggatctc gccccaccgg tgccggtcgt acatccgctg
     3961 cgcgccctcg agcgtcggca cgagcgtcgc gagcgagcgc tggttcgggc ccgcgtcctc
     4021 gggcatcccg ttccagcggg gctcctcgac caaggcgaag ttcaccttcg cgcacggacc
     4081 ggccatggcg atcgcctcga cgtcggcacg gaagtcctcg gagagctgcg acttctcgac
     4141 caggccgagg aaggtgcgct tcgggtcggc gttcgagagc acggtgcggg ccgggatctc
     4201 ggtgccgtcc tcgagcgtca cccccgtcgc ccgaccgtcg cgaacgtcga tccgcgcgac
     4261 ctcggagccc gtacggatct cgccgccggc atcgcggacg gccgaggcca tcgcctgggt
     4321 gatcgaaccc atcccgccga tcacgtggcc gttgaagccc tggatgtggt gctcgccgcc
     4381 ggagagcatg tggaacagca acccgatcgc cgtccccggc cgatagggcg gcgcgtgcat
     4441 cccgtagacg ttgttcgcga ggtacaggcg ccgcacctcg tcggactcga aatgccgttc
     4501 gacgaactcg ccgagcgagc cggtcgcgaa ccgcaccaga tccgagagct ggtcgcccga
     4561 caggtgccgg aatcgacgga acagtcgccg cccctcgcga agcttgctcc agccggtcgc
     4621 gtacaggttc ggcggctcct cgaggaagaa cggctggagc gagcgggcga gctcgtgcag
     4681 ccgttcggtc acgcgcgcga acgcgtccgc gtcgcgggcc gagatccgcc ggatctcctc
     4741 cgccgtgcgc tcgtgcgtcg accaccacgg cagcacccgg gcgtcctcga
//
LOCUS       DATBRK010000335         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1795295, whole genome shotgun sequence.
ACCESSION   DATBRK010000335 DATBRK010000000
VERSION     DATBRK010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795295"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..742
                     /locus_tag="VIH70_09965"
     CDS             329..742
                     /locus_tag="VIH70_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116726.1"
                     /translation="MPAARQFVLALMAAVLWVVVAAVLSPSPAWLFGGGALFLAFVYA
                     GVLRDPTVTRRGPAALVDTSFLVLELVLFLLVGGYFRLWMVVAFVLATMLASAAGLFT
                     QGRERRQIAMYTAIGFPVILVLTVLVFVKSNVTFF"
     gene            755..1057
                     /locus_tag="VIH70_09970"
     CDS             755..1057
                     /locus_tag="VIH70_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116727.1"
                     /translation="MDYPDDYPPPDPDGRPHVWLDFNVGTPHEWDLGRLASSAGSVKE
                     QQLELSEGLGVVLFEDDGDEWMLVDAIVDRFNPQRNGSWIVRIDWSTFRRIPKPGR"
     gene            complement(1173..2105)
                     /locus_tag="VIH70_09975"
     CDS             complement(1173..2105)
                     /locus_tag="VIH70_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116728.1"
                     /translation="MVALLTACDSSGKGAMAMPSRLWSIDGDGEVGVHAAIEIPPDAI
                     GMAAAAGEVWIIEPDGVTKVHPAKGIHRTIPLPGRPTTIEIDTKLGVWIGGRGPSDDE
                     LLWNIDPDTASVVRTVHLPNTPESLAIAASASPPTVWVISPAVGTILQIDATSGGIVG
                     SNYFGEDMSDIATDSSLAWVAFDQDQTVMPVPSLDLPSPFFHEGQKYSVPGADALAAT
                     EDTLWVLDAGGERVFPVDLLADHVGTPISVGSRPTTIGLDGAYLWVANNRSITRIDRT
                     SHGRSTIAFSRPLSFIATTQGPRTMVWVLADRTA"
BASE COUNT          455 a          802 c          822 g          555 t
ORIGIN      
        1 cagtgcgaat acgtttccga gaacaatccc gacgtgatca aggttctcct gcggatcaca
       61 gagaaagacg gctactggtg ggtggaatgt gccggctgcg aaagcggttg gcaggttccc
      121 ttctacgccc aggagagggt cgggtagggg gttcggcggg accttggcgt agaggggagg
      181 tcactccgcc cgcctaaagc gctaagggat ggtcctgcca atgggttggg ccgggggagc
      241 gatacctctg gcccaccgcc ctggcagcat ccgtgttggg catgcgggcc ccttcatcct
      301 cggcgtgcgt atgccgatta catagccgat gccagcagct cgacagttcg ttctggcctt
      361 gatggccgct gttctttggg tagtcgtcgc ggcggtcctg tcgccttcgc ccgcgtggct
      421 gttcggaggc ggggctctgt ttctggcgtt cgtctatgca ggcgtgctcc gcgacccgac
      481 ggttacacgg cgtggacccg ctgccctcgt cgacacgtcc ttcttggtgc tcgagctcgt
      541 cctcttcctg cttgtcggtg gctacttcag gctgtggatg gtcgtagctt tcgtcttggc
      601 gacgatgctt gcctcggcgg cgggcctctt cactcaaggg cgcgagagac gccagatagc
      661 gatgtacacg gcgattggct tcccagtgat cctggtgttg accgtgctcg tgttcgtcaa
      721 gagcaacgtg acgttcttct gaacgcctga ggtcatggac tatccggacg attatccgcc
      781 tcccgacccg gacggccgac ctcatgtctg gctcgatttc aatgtcggta cgccacatga
      841 atgggatctc ggacgcctcg ctagcagcgc gggaagcgtc aaggaacaac agcttgaact
      901 tagcgaaggc ctcggggtag tcctgttcga ggacgacggg gacgaatgga tgctcgtcga
      961 cgcgatcgtg gatcggttca atccgcaacg caacggtagc tggatcgtac gcatcgattg
     1021 gtcgacgttt cgccgcatac caaagcccgg tcgctagacg ctacttccgc tccatctccg
     1081 cgagcaagcc gaacacgacc gcccgcgccg ccttgtcctc tccaggtcga tcagcaactc
     1141 atttgggctt cctgagcggt gggtgagccg cctcacgcag ttcgatcggc taggacccaa
     1201 accatcgttc tcgggccctg ggtggttgcg atgaacgata gaggtcgtga aaacgcgatg
     1261 gtgctgcggc cgtgactcgt tcgatcgatc cgggtgatgg atcgattgtt ggccacccag
     1321 aggtacgctc cgtccaagcc gatggtggtt ggtcgcgaac cgacggagat gggcgttcca
     1381 acgtgatccg ccaagaggtc gacgggaaag acccgctcgc ctccggcgtc gagcacccat
     1441 agcgtgtcct cggttgccgc gagggcgtcg gcgcctggga cggagtactt ctgtccctca
     1501 tggaagaagg gagagggcaa gtccaacgat gggaccggca taacggtctg atcctgatcg
     1561 aacgctaccc acgcgaggga ggagtcggtc gcgatgtcac tcatgtcctc cccgaagtag
     1621 ttggacccca cgatcccccc gctcgtcgca tcaatctgaa ggatcgtccc gacggccggt
     1681 gagattaccc ataccgtcgg cggtgatgcc gacgccgcga tagccaagga ttccggtgtg
     1741 ttcggcaggt gaacggtccg gaccacgctg gccgtatcgg gatcgatgtt ccaaagcaat
     1801 tcgtcgtcgc ttggacctcg gcctccgatc cacactccga gtttggtatc gatctcgatt
     1861 gtcgtggggc ggcccggtag cgggatcgtc cgatggatac ccttcgccgg atgaactttg
     1921 gtcactccat ccggttcgat gatccaaacc tcgcccgctg cagcggccat cccgatggca
     1981 tccggcggaa tctcaatggc ggcatggaca cctacctctc cgtcgccatc gatactccag
     2041 agtctggatg gcatcgccat agcgccttta cccgacgagt cgcacgccgt cagcagagcc
     2101 accatcccgg cggcgattat cgaggggatg agaacggctt cagcgcaagc actcctctgt
     2161 gcaacaccgc cgtgttcgta ttgtgcattg gcacgttcgg aggatctgag gtgagtcgag
     2221 tttgacacga ctgccttacg ccaaagcgtg agtgtctgac acctatcgac gcatcctgct
     2281 acctccgctc catctctcgg agcagccccc agatcaccgc cttggcgtac ttgtcctcct
     2341 ccaggtcgat cagcagctcg gcaaggtgcg aggggcagcg ggtgcggtga cgcatcatgt
     2401 gaaggcagtg tgtctgccta tcaggggatc gtgacgttat cgagggcgca tcgtgtcggg
     2461 tcttcgcctc ggccctgttg ttgagaactc gagcccgacc cgcccccgcc gtaagttcct
     2521 tcgatcggcc ggtaacgaac ttggcatttg acccttgtcc ctgcggggcc gtccgcttcg
     2581 atgcggatga actcgtcagg tccctcgaac tcgcgactcc tccacgggag cgtg
//
LOCUS       DATBRK010000336         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_63066, whole genome shotgun sequence.
ACCESSION   DATBRK010000336 DATBRK010000000
VERSION     DATBRK010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63066"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..509)
                     /locus_tag="VIH70_09980"
     CDS             complement(153..509)
                     /locus_tag="VIH70_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY8116729.1"
                     /translation="MSGSTTERARAGEDAALRVYERRGFAVVARNWRCGLGELDLIIV
                     RRDLLVVCEVKTRSGPAFGGGYEAVTWAKRKKVRTLADAFLQGNAFEHARVRFDVASV
                     WLGPRGADVEIFEDAF"
     gene            complement(609..914)
                     /locus_tag="VIH70_09985"
     CDS             complement(609..914)
                     /locus_tag="VIH70_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 family protein"
                     /protein_id="HEY8116730.1"
                     /translation="MNPEEDDIERFEEERELELLREYRDIVPMFAYVVETERRFYLAN
                     TVELNQREDGWVEVVLRDAWVWDMFRPARLVSNVRVLTRHDVNVEELRQEDTMQLPE"
     gene            complement(911..1555)
                     /locus_tag="VIH70_09990"
     CDS             complement(911..1555)
                     /locus_tag="VIH70_09990"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY8116731.1"
                     /translation="MDPLVLDLGRYERGLREQGFRLIAGVDEAGRGALAGPLVAAAVI
                     LPAEFDLDGINDSKVLTALQRDAAFERIVRHALARSVCRCTPTRIDRRGLHKTNVWLL
                     RRCITTLPMAPDYVLTDGFPVKRLGIPNLSIKKGDAVTASVAAASIVAKVTRDRMMDR
                     YHRRFPAYGFDHNRGYGTDEHREAIRRHGPSPIHRYSFKGLRTGYYDGDRVEEP"
     gene            complement(1543..>1918)
                     /gene="lepB"
                     /locus_tag="VIH70_09995"
     CDS             complement(1543..>1918)
                     /gene="lepB"
                     /locus_tag="VIH70_09995"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8116732.1"
                     /translation="GRPGPDRGIVGGFVHWLSSTLGIERPEHEDFIKRVIGLPGETVE
                     LREGRLYVDGVLIHEPYLEGAVDTRDYGPVRVPKGALFVLGDNRLNSNDSRFGLGFVP
                     VDRVVGRAFAIVWPPSRVGWIH"
BASE COUNT          288 a          709 c          642 g          279 t
ORIGIN      
        1 gcccgcgccg ggcagcccgg tcagggtgag cgacgggagt ccgcgaccga cgtgcgcctc
       61 gacggcgacg agatggccgc ggacgccgac gacggtgacg ccgttgacgc gtccgtacac
      121 ggtgggccga ccctagcggc agccaccgac acctagaacg cgtcctcgaa gatctcgacg
      181 tccgcgccgc gcggaccgag ccacacgctc gcgacgtcga agcgcactcg cgcgtgctcg
      241 aacgcgttcc cttgcaggaa cgcgtccgcg agcgtccgta ccttcttgcg cttggcccac
      301 gtcaccgcct cgtagccgcc gccgaacgcc gggcccgatc tcgtcttgac ctcgcagacg
      361 accagcaggt cgcgtcgcac gatgatgagg tcgagctcgc ccaggccgca gcgccaattc
      421 cgcgcgacca cggcgaatcc ccgtcgctcg tagacgcgga gggcggcgtc ctcgccggcg
      481 cgggcgcgtt cggtcgtgga tccgctcacc ccgcgacgct agaccgcgtc gccgacacgc
      541 cgaggtcggt cccccacgcg aagggcccgg tgcaggcagg tcggtccggg cgacgccgtg
      601 ttcggtcgct actcgggaag ctgcatcgtg tcctcctgac gcagctcctc cacgttcacg
      661 tcgtggcggg tgagcacccg cacgttcgac acgaggcggg ccgggcggaa catgtcccac
      721 acccaggcgt cgcggagcac cacctcgacc cagccgtcct cgcgctggtt gagctcgacc
      781 gtgttcgcga ggtagaagcg ccgctcggtc tccacgacat aggcgaacat cggcacgatg
      841 tcgcggtact cgcggagcag ctcgagctcg cgctcctcct cgaaacgctc gatgtcgtct
      901 tcttcggggt tcacggctcc tccaccctat cgccgtcgta gtaaccggtc cgcaggccct
      961 tgaacgagta ccgatggatc ggcgacgggc cgtggcgcct gatcgcctcg cgatgctcgt
     1021 cggtcccgta gccccggttg tggtcgaacc cgtaggcggg gaacctgcgg tggtagcgat
     1081 ccatcatccg gtcccgggtc accttggcca cgatcgaggc ggccgcgacc gacgcggtga
     1141 ccgcgtcccc cttcttgatc gacaggttcg ggatcccgag ccgcttcacc gggaagccgt
     1201 ccgtcaggac gtagtcgggt gccatgggca gggtggtgat gcaccgacgg agcagccaga
     1261 cgttggtctt gtgcaggccc cggcggtcga tccgcgtcgg cgtgcaccgg cagacggaac
     1321 gagcgagggc gtggcgcacg atgcgctcga aggccgcgtc gcgctggagc gcggtcagca
     1381 ccttagagtc gttgatcccg tccaggtcga actccgccgg caggatcacg gccgcggcca
     1441 cgagcgggcc ggcgagggca ccgcgtcccg cctcgtcgac gccggcgatc aggcggaagc
     1501 cctgctcccg caggccgcgc tcgtagcgcc cgaggtcgag gactagtgga tccacccgac
     1561 cctcgacggt ggccacacga tcgcgaacgc cctcccgacc accctgtcca ccgggacgaa
     1621 cccgaggccg aaccgcgagt cgttcgagtt cagccggttg tcgcccagga cgaagagcgc
     1681 gcccttcgga acccgcaccg ggccgtagtc cctcgtgtcg accgccccct ccaggtaggg
     1741 ctcgtggatc aggaccccgt cgacgtacag tctcccctcc cgcagctcga ccgtctcgcc
     1801 ggggagcccg atcacccgct tgatgaagtc ctcgtgctcg ggccgttcga tgccgagcgt
     1861 gctcgacaac cagtggacga acccgccgac gatcccgcga tccgggccgg ggcgcccc
//
LOCUS       DATBRK010000337         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2224493, whole genome shotgun sequence.
ACCESSION   DATBRK010000337 DATBRK010000000
VERSION     DATBRK010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224493"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VIH70_10000"
     CDS             <1..394
                     /locus_tag="VIH70_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116733.1"
                     /translation="GLCPSVSWGKDYPDGFTFGPPLFSRDAIGPESCCNDPMVGITPE
                     MLQERGYDPSTEVPSAEDQIDACIPLSGDKRLQCWAELDQYLMEDVTPWIPYLFDNNV
                     VVASDRIVAWSFDQFAGLPAIDRLALAQ"
     gene            623..1393
                     /locus_tag="VIH70_10005"
     CDS             623..1393
                     /locus_tag="VIH70_10005"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY8116734.1"
                     /translation="MSDPVRDRWAAGEVALNAWSTFGGASAASVLAAAGFDAVTVDLQ
                     HGEHTLERLGEVVGEIDRAGAVPFVRLGWNDPAIVMRVLDLGARGVICPMVDSAEDAA
                     AFVRHCRYPPLGARSYGPVRSAFGTGREQVDAANAAILAFAQIETAAGWANVEGICAT
                     PGLDGVYVGPADLSLALGFEGFADLGAHDLLEALDRVVRAASEHAIVPGIHAPSIERT
                     VEMARRGFRFVGAAGDIELLRGGAAESIARTRADARTL"
     gene            complement(1390..1803)
                     /locus_tag="VIH70_10010"
     CDS             complement(1390..1803)
                     /locus_tag="VIH70_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8116735.1"
                     /translation="MRSTELATIEFGQAERATVATIGGEVDLSNADRLLDELMARVGT
                     TPWLILDLTACAYMDSAGLRMVARVDERCRTAGAGLRVVVDPGGSIDRVLEMTHMDEV
                     LTVDPLLRDALAAVREEVDGAEFGVEGADGGAIEP"
     gene            complement(1800..>2441)
                     /locus_tag="VIH70_10015"
     CDS             complement(1800..>2441)
                     /locus_tag="VIH70_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8116736.1"
                     /translation="RSVPLGLPFDLPRPQAHERLGPGSILVVYTDGLVERREEPLDRG
                     LQRLAEVASRVRAGSLTEISDGLLELVADDRHDDVALLTIGPRRPVEVFRRTFPANAH
                     ELATMRADLRTWLERSGLPTETREDVVLACTEAATNAIEHAYIGRRGDVLIEAESEDG
                     ALQVSVTDHGRWRHPRPDDSRGRGLELVRAVIGDVDVQRGEGGTTVRMRVGIP"
BASE COUNT          344 a          870 c          877 g          350 t
ORIGIN      
        1 gggcctgtgc ccgagcgtct cgtggggcaa ggactatccg gacggcttca cgttcggccc
       61 gccgctgttc agccgggacg cgatcggccc cgagtcgtgc tgcaacgacc cgatggtggg
      121 catcacgccc gagatgctgc aggagcgcgg gtacgacccc tccaccgagg tcccgagcgc
      181 cgaggaccag atcgacgcct gcatcccgct ctcgggtgac aagcggctcc agtgctgggc
      241 cgagctggac caatacctga tggaggacgt gaccccgtgg atcccgtacc tgttcgacaa
      301 caacgtggtc gttgcctccg accggatcgt cgcgtggagc ttcgaccagt tcgccggtct
      361 cccggcgatc gaccggctcg cgctcgcgca gtgaggtgag cggtgaccgc tcggtgaagt
      421 gagcggcgac cgggaccgag gacgacgtgg aaaggtccgg agctcggatc ggagagaggg
      481 tcaaagacct gagctccgga ccttccaccc gaacgtacgc ccggaaccaa cggagcgctt
      541 gcggttccct gtcgacgagt gacgggtccc tgccgagggt tcgctcggcg cgtgtgccac
      601 gatcgtgtct acgatgcgcc ccatgtccga tccggttcgc gatcggtggg ccgcaggcga
      661 ggtggccctg aacgcgtggt cgacgttcgg gggcgcgtcg gcggcgtcgg tcctggcggc
      721 ggccggcttc gacgccgtga cggtcgacct ccagcacggc gagcacacgc tcgagcgcct
      781 cggggaggtg gtcggcgaga tcgatcgcgc cggcgccgtc ccgttcgtcc gcctcggctg
      841 gaacgatccc gcgatcgtga tgcgcgtgct ggacctgggg gcgcgcggcg tgatctgccc
      901 gatggtggac agcgccgagg acgcggccgc gttcgtacgc cactgccggt acccaccgct
      961 cggcgcccgg agctacgggc cggtcagatc ggcgttcggg acaggtcgcg agcaggtcga
     1021 cgcggcgaac gccgcgatcc tagcgttcgc gcagatcgag accgcggcgg ggtgggcaaa
     1081 cgtcgaaggg atctgcgcca cgccggggct cgacggcgtg tacgtcggcc ccgccgacct
     1141 gagcctcgcg ctcgggttcg agggcttcgc cgacctcggc gcgcacgatc tgctcgaggc
     1201 gctcgatcgg gtcgtgaggg ccgcctccga gcatgccatc gtccccggca tccacgcacc
     1261 gtcgatcgag cggacggtcg agatggctcg gagggggttc cggttcgtgg gtgcggcggg
     1321 cgacatcgag ttgttgcgag gcggcgccgc cgagagcatc gcacgcaccc gggccgacgc
     1381 ccgcaccctc tagggctcga tcgctccccc gtcggcgccc tcgacgccga actcggcacc
     1441 gtcgacctcc tcgcgcaccg cagcgagcgc gtcccgcagg agcgggtcga cggtcagcac
     1501 ctcatccatg tgggtcatct cgaggacgcg atcgatcgag cctcccgggt ccaccaccac
     1561 cctgagcccc gcacccgcgg tgcggcatcg ctcgtcgacc cgagcgacca tccgcagccc
     1621 ggcgctgtcc atgtacgcgc acgcggtgag atcgaggatc agccacggcg tcgtgccgac
     1681 gcgcgccatc agctcgtcga gcagccgatc ggcgttggac aggtcgacct cgccgccgat
     1741 cgtggcgacg gtcgcgcgtt cggcctgccc gaactcgatc gtcgcgagct cggtcgacct
     1801 cacgggatcc cgacgcgcat ccggaccgtg gtgccgccct cgccccgctg cacgtcgacg
     1861 tcgccgatca ccgcccgaac gagctcgagg cctcgacccc gcgaatcgtc cgggcgcggg
     1921 tgacgccacc ggccgtggtc ggtcaccgag acctggagcg ccccgtcctc ggactcggcc
     1981 tcgatcagca cgtcgccgcg gcgaccgatg tacgcgtgct cgatggcgtt ggtcgccgcc
     2041 tccgtgcacg cgagcaccac gtcctcccgg gtctcggtgg gcagaccgga gcgttcgagc
     2101 cacgtgcgga ggtcggcacg catcgtggcg agctcgtggg cgttcgcggg gaaggtccgg
     2161 cggaacacct ccaccgggcg ccggggtccg atcgtcagga gcgcgacgtc atcgtgacga
     2221 tcgtcggcga cgagctcgag cagcccgtcg gagatctcgg tcagcgagcc cgcgcgcacg
     2281 cgcgaggcca cctcggcaag ccgctggaga ccccggtcca gcggctcctc gcggcgttcg
     2341 accaggccgt ccgtgtacac gaccaggatc gagccggggc cgagccgctc gtgcgcctgg
     2401 ggtctcggca ggtcgaacgg caggccgagc ggcaccgatc t
//
LOCUS       DATBRK010000338         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_781606, whole genome shotgun sequence.
ACCESSION   DATBRK010000338 DATBRK010000000
VERSION     DATBRK010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781606"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VIH70_10020"
     CDS             complement(<1..282)
                     /locus_tag="VIH70_10020"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116737.1"
                     /translation="MQDAIGRLHWIVIDTVDPARIAPFWCALLGVRERGWFGDDYLML
                     TADGGAPPVAFQRVPEAKSVKNRLHVDLAVDDLDDAFAKIVALGGSAVSD"
     gene            complement(356..>1636)
                     /locus_tag="VIH70_10025"
     CDS             complement(356..>1636)
                     /locus_tag="VIH70_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8116738.1"
                     /translation="RSRELFERAKANLLAGVPMPWMSEWAGPYPVFVAEARGARFIDV
                     DGREYVDLCLGDTGSMTGHGPKEAVDAIAEQAAKGITHMLPTEDAAWVGAELQRRFGL
                     PFWQFCLTATDANRFTIRLARAITGRPKILVFNYCYHGSVDETIVTIEDGVPGPRVGN
                     VGAPVDPTETTRVVEFNDVDALEAALAHEDVACVLAEPALTNIGIVLPEPGLHDALRT
                     LTREHGTYLVIDETHTICAGPGGATRAWGLDPDFLTVGKPLASGVPAACYGMSAEVAG
                     RTDTFMRSLPTTDVGGIGGTLSGNALSLAAMRATLEHVLIDSAFERMIALGERWADGV
                     SAAIREHDLPWEVQRIGCRAEYWFTPTPPRTGGDAAAADDHELARFTHLYALNRGILL
                     TPFHNMALMSPATTEAEVDAHSTVFDEMAAELTG"
BASE COUNT          228 a          591 c          573 g          244 t
ORIGIN      
        1 gtccgagacg gccgaaccgc cgagcgcgac gatcttcgcg aaggcgtcgt cgaggtcgtc
       61 gaccgcgagg tcgacgtgga gccggttctt gaccgacttg gcctccggca cccgctggaa
      121 cgcgaccggc ggcgcgccgc catcggccgt cagcatcaga tagtcgtcgc cgaaccaccc
      181 ccgttcccgg acgccgagga gcgcgcacca gaacggcgcg atcctcgcgg gatcgacggt
      241 gtcgatcacg atccagtgga gccgtccgat cgcgtcctgc atgagccgag cgtacgacgg
      301 cgagctcgcg cggcgcttgc ccaagcttgc cctgtcggcg accgcgacgg acggttcagc
      361 cggtcagctc ggccgccatc tcgtcgaaca cggtcgagtg ggcgtcgact tcggcctcgg
      421 tggtcgcggg ggacatcagg gccatgttgt ggaacggcgt gagcaggatg ccgcggttga
      481 gcgcgtacag gtgcgtgaac ctcgcgagct cgtggtcgtc ggcggccgct gcgtcgcccc
      541 cggtccgcgg cggcgtcggc gtgaaccagt actcggcccg gcacccgatc cgttgcacct
      601 cccacggcag gtcgtgctcc ctgatcgccg ccgagacgcc gtcggcccat cgctcgccga
      661 gcgcgatcat ccgctcgaac gccgagtcga tcaggacgtg ttcgagcgtg gcccgcatcg
      721 cggccagcga gagcgcgttg cccgaaagcg tcccgccgat ccccccgacg tcggtcgtgg
      781 ggagcgagcg catgaaggtg tcggtgcgtc cggcgacctc ggcgctcatc ccgtagcacg
      841 ccgcgggcac cccgctcgcg agcggcttgc cgaccgtgag gaagtcgggg tcgagccccc
      901 atgcccgcgt cgcgccgccg ggcccggcgc agatcgtgtg ggtctcgtcg atcaccaggt
      961 acgtgccgtg ttcgcgcgtc agggttcgga gcgcgtcgtg caaccccggc tcgggcagca
     1021 cgatcccgat gttcgtgagc gccggttcgg cgagcacgca ggccacgtcc tcgtgcgcga
     1081 gcgccgcctc cagcgcgtcc acgtcgttga actcgaccac cctcgtggtc tcggtcgggt
     1141 cgacgggcgc tccgacgttc ccgacgcggg ggccagggac gccgtcctcg atcgtcacga
     1201 tcgtctcgtc caccgagccg tggtagcagt agttgaaaac caggatcttc ggccggccgg
     1261 tgatcgctcg cgccagccgg atcgtgaacc ggttcgcgtc ggtggcggtc aaacagaact
     1321 gccagaaggg caggccgaag cggcgctgca gctcggcgcc cacccacgcc gcgtcctcgg
     1381 tcggcagcat gtgggtgatg cccttggccg cctgctcggc gatcgcatcg accgcctcct
     1441 tcgggccgtg gccggtcatc gacccggtgt cgcccaggca gaggtcgacg tactcgcggc
     1501 cgtcgacgtc gatgaacctg gccccgcgag cctcggccac gaagacgggg tacggcccgg
     1561 cccactcgct catccacggc atcgggaccc ccgccaggag gttggccttg gcccgctcga
     1621 acagctcacg cgagcg
//
LOCUS       DATBRK010000339         4045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_781721, whole genome shotgun sequence.
ACCESSION   DATBRK010000339 DATBRK010000000
VERSION     DATBRK010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781721"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VIH70_10030"
     CDS             <1..544
                     /locus_tag="VIH70_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY8116739.1"
                     /translation="ETAERFAAGILAHTPGLLALTAPSFNSFRRLLPQHWSSAFVCWG
                     PDNREATVRVPSTFWGLEETSTNLEFKPSDASCNPYIAFAGLIAAGLDGIERGLGLPE
                     PMLVDPATLTDAERERAGAVRYPETLGAALDALERDEVLTGAMGDLLTASHVAVRRSE
                     FEAYGAMDEAEQYRGHFEKY"
     gene            complement(564..1712)
                     /locus_tag="VIH70_10035"
     CDS             complement(564..1712)
                     /locus_tag="VIH70_10035"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8116740.1"
                     /translation="MPIVDAHTHPYRLEELLERDPGGFDTRAMFLGESFASSSRVQED
                     LWPFVDALTGSTVYGIAMHRWLAEHLGCEPDRRAVVEARAAALRADPVAYTKGLLDAA
                     GVVAVLSDEGFPQPPVPAEEFERTIGVRVHRVARLEPWILVHREGTFDDLVAGVDDEA
                     RRAAADPNCVAFKSIVAYRTGLDVGDPSEADAREAFAGWRGDEWRETRDHAKPVRDFL
                     IRRTLGVAKELGRAFHFHCGGGDPDIDLIHAKPENLFPLLVDVQDQPVVLVHSGYPWI
                     RESAYIASVLPNVYLELSELIPWGFGQVEWALEMLVGTVPAAKLLYGSDEAGEPESFW
                     ISALLARRALERVLEGFVERDYVATGDAERLGAMVLGDSCRALHGLTE"
     gene            complement(1774..3132)
                     /locus_tag="VIH70_10040"
     CDS             complement(1774..3132)
                     /locus_tag="VIH70_10040"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY8116741.1"
                     /translation="MSVARRLAAAGVEAVALAMVDPAGITRVKCIPIRRFEEVARFGV
                     GLSNVFSVFLVNDGITSSPGIEGPSGDTRLVPDPASAVALAASPGWALAAVDQRDQEG
                     DPWPPCGRTFARRMLERLSAQGLELRGTYELEFFLGNRVAPREADEPEPIPAHVGPGY
                     SAQVLADQEPFATDVIRALERQGTGVMQFHPEYSTGQFEISVPHRAGIAVADTNLVAR
                     HTIRAVARANGLAVSFAPVVFAGLVGNGDHLHLSLWDRRGRNRFAGGDGPEGMTREAE
                     AFAAGILGSLPAIVAVSCPSVASYLRLQPHRWAGAFQAWGRENREAGLRFVTGMVGSR
                     SEAANLEVKPVDGGGNPYLVLGSIVAAGLDGLGRDLRLPEPTIEDPASLSAAERRRRG
                     ISPLPSSLGAAIAELERSAVLREAMGDMLFDSFVATRKGELAAFDGMDDDAVVRAHRW
                     RY"
     gene            complement(3195..>4045)
                     /locus_tag="VIH70_10045"
     CDS             complement(3195..>4045)
                     /locus_tag="VIH70_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY8116742.1"
                     /translation="TVRNDFVPTIRRIVEEMPRVGVDVITANCEYAGSQWEINFAPGR
                     GLRGPDNAFTFKNGVKEIAKQDGYLATFMSKPFATSAGSGCHTHLSLLHGDSGENAFA
                     DEGDPNGMNDTARSFIAGLLTYARAIDAVIAPTVNCLRRRRRHTFSPTNVSWGYEDRS
                     ALVRVKGGSPASKHIEYRAPSALSNPYLVGAALIQAGLRGIEDKLTAPEPSEPGVPAE
                     EDETLEKLPTDLPGSLDALEAEPAARDFLGDEFIAAYTTMRRYELSRLADHVSDWERM
                     EYLELF"
BASE COUNT          567 a         1485 c         1431 g          562 t
ORIGIN      
        1 cgagaccgcc gaacgcttcg cggccgggat cctcgcgcac acgccgggtc ttctcgcgct
       61 caccgccccc tcgttcaact cgttccgccg gctcctgccg caacactgga gctcggcgtt
      121 cgtgtgctgg ggaccggaca accgggaggc cacggtccgc gtgccgtcca cgttctgggg
      181 gctcgaggag acgtcgacca acctcgagtt caagccgtcg gacgcgagct gcaacccgta
      241 catcgccttc gcggggctga tcgcggccgg tctggacggg atcgagcgtg gcctgggact
      301 ccccgagccg atgctggtcg accccgcgac gctcacggac gccgaacgcg aacgtgctgg
      361 agccgtgcgg taccccgaga cgctcggcgc ggccctggac gcgctcgaac gcgacgaggt
      421 gctcacgggc gcgatgggcg acctgctcac cgcgtcgcac gtcgccgtgc gccgctccga
      481 gttcgaggcc tacggggcga tggacgaggc ggagcagtac cgcggccact tcgagaagta
      541 ctgaccttcg gcccggtggt ggctcactcg gtcaggccgt gcagcgcgcg gcagctgtcg
      601 ccgagcacca tcgcgccgag cctctcggcg tcgccggtgg ccacgtagtc gcgctcgacg
      661 aacccctcca ggacccgttc gagcgcgcgt cgggccagga gcgccgagat ccagaacgac
      721 tccggctccc ccgcctcgtc ggagccgtac aggagcttgg ccgccggcac cgtccccacc
      781 agcatctcga gcgcccactc cacctgcccg aacccccagg ggatcagctc ggacagctcg
      841 aggtagacgt tcgggagcac cgaggcgatg taggcggact cgcggatcca ggggtagccg
      901 ctgtggacga gcaccaccgg ctggtcctgc acgtcgacga gcagcgggaa caggttctca
      961 ggcttcgcgt ggatcaggtc gatgtcggga tccccgcccc cgcagtggaa gtggaacgcg
     1021 cgacccagtt ccttggcgac cccgagcgtg cgccggatca ggaagtcccg gaccggcttt
     1081 gcgtggtccc gcgtctccct ccactcgtcc ccgcgccagc ccgcgaaggc ctcacgggcg
     1141 tcggcctcgc tcgggtcccc gacgtccagt cccgtccggt acgcgacgat cgacttgaag
     1201 gcgacgcagt tcggatcggc ggcggctcgc cgcgcctcgt cgtcgacgcc ggcgaccagg
     1261 tcgtcgaacg tgccctcgcg gtgcacgagg atccagggct cgagccgggc gacccggtgg
     1321 acgcgcacgc cgatcgtgcg ttcgaactcc tccgccggga ccggcggctg ggggaacccc
     1381 tcgtccgaca ggaccgcgac gacgccggca gcgtcgagca gccccttggt gtacgcgacg
     1441 ggatcggcgc gcagggccgc cgcccgcgcc tcgaccaccg cccgccgatc cggttcgcac
     1501 ccgaggtgct cggcgagcca ccggtgcatc gcgatcccat agaccgtcga cccggtcagg
     1561 gcgtccacga acggccacag gtcctcctgc acccgggagg acgacgcgaa gctctcgccg
     1621 aggaacatcg cgcgggtgtc gaacccgccg gggtcgcgtt cgagcagctc ctccagacgg
     1681 tacgggtggg tgtgcgcgtc gacgatgggc acgcccgaca gatcgatccc gaccaatgtc
     1741 ggtcccctcc ttcttctggc cctcggtcgc ctctcagtac cgccagcggt gcgcgcggac
     1801 gacggcatcg tcgtccatcc cgtcgaacgc cgcgagctcg cccttgcgcg tggcgacgaa
     1861 cgaatcgaag agcatgtccc ccatcgcctc gcgaaggacg gccgagcgct cgagctccgc
     1921 gatcgcggcc ccgagcgacg acgggagcgg cgagatcccg cgcctgcgtc gctcggccgc
     1981 ggagagggac gccggatcct cgatggtcgg ctcggggagc cgcaggtcgc gtccgagccc
     2041 gtcgaggccg gcggcgacga tcgacccgag gaccaggtac gggttgccgc ccccgtccac
     2101 gggcttgacc tccaggttcg ccgcctccga gcggcttccc accatccccg tcacgaaccg
     2161 gagcccggcc tcgcggttct cgcggcccca cgcctggaac gcgcccgccc accggtgcgg
     2221 ctggagccgc aggtagctcg cgaccgacgg gcacgagacc gcgacgatcg ccggcaggga
     2281 cccgaggatc ccggcggcga acgcctcggc ctcgcgcgtc atcccctcgg gcccgtcgcc
     2341 gccggcgaac cggttgcgcc cgcgccggtc ccacaggctg aggtgcaggt gatcgccgtt
     2401 gccgaccagg ccggcgaaca cgaccggggc gaacgagacc gcgagcccgt tcgcgcgcgc
     2461 gaccgcccgg atcgtgtgcc gggctacgag gttggtgtcg gcgaccgcga tcccggcgcg
     2521 gtgcggcacg gagatctcga actgcccggt cgagtactcg gggtggaact gcatcacgcc
     2581 ggtcccctgc cgttcgagcg cgcggatcac gtcggtcgcg aacggctcct ggtcggcgag
     2641 cacctgggcg ctgtagccgg gaccgacgtg ggcggggatc ggctccggct cgtcggcctc
     2701 gcgaggcgcg acgcggttcc cgaggaagaa ctcgagctcg tacgtgcccc ggagctcgag
     2761 cccctgcgcc gacaggcgtt cgagcatgcg tctcgcgaac gtgcgcccgc acggcggcca
     2821 ggggtccccc tcctggtcgc gctggtcgac ggcggcgagc gcccacccgg gcgaggcggc
     2881 cagcgcgacc gccgaggcgg gatcggggac gagccgcgtg tcgccgctcg ggccctcgat
     2941 gcccggcgac gaggtgatgc cgtcgttgac caggaagacc gagaagacgt tcgacagccc
     3001 gacgccgaac cgcgcgacct cctcgaaccg ccggatcggg atgcatttca cacgcgtgat
     3061 accggcgggg tcgaccatcg cgagcgcgac cgcctccacc ccggccgccg ccaggcgtcg
     3121 cgccaccgac acggccccgt cgctcgtgcg ccgggccggg gccgtgcgcg ccgttcgggg
     3181 cttggcggcg gggctcagaa cagctccagg tactccatgc gctcccagtc gctcacgtga
     3241 tcggcgagcc gggagagctc gtaccgccgc atcgtcgtgt acgccgcgat gaactcgtcg
     3301 ccgaggaagt cgcgagcggc gggctcggcc tcgagcgcgt cgagcgaccc ggggagatcg
     3361 gtcggcagct tctcgagcgt ctcgtcctcc tcggcgggga ctcccggctc ggacggttcg
     3421 ggcgccgtga gcttgtcctc gatcccgcgg agcccggcct ggatcaacgc ggcgccgacg
     3481 aggtaggggt tcgacagcgc cgagggcgcc cggtactcga tgtgcttgga ggcggggctc
     3541 ccgcccttga cccggaccag cgccgagcga tcctcgtatc cccaggaaac gttcgtcggg
     3601 ctgaacgtgt gtcgccggcg gcggcggagg cagttcacgg tgggcgcgat caccgcgtcg
     3661 atcgcgcgcg cgtacgtgag gaggccggcg atgaacgaac gcgcggtgtc gttcatgcca
     3721 ttcggatcgc cctcgtccgc gaacgcgttc tcccccgagt ccccgtgcag gagcgagagg
     3781 tgtgtgtggc agccgctgcc cgcggaggtc gcgaacggtt tgctcatgaa cgtcgcgagg
     3841 tagccgtcct gcttcgcgat ctccttgacg ccgttcttga acgtgaacgc gttgtccggt
     3901 ccgcgcaggc cacgtccggg cgcgaagttg atctcccact gcgacccggc gtactcgcag
     3961 ttggcggtga tgacgtcgac gccgaccctc ggcatctcct cgacgatccg ccggatcgtc
     4021 gggacgaagt cgttgcggac cgtgt
//
LOCUS       DATBRK010000340         9690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2225076, whole genome shotgun sequence.
ACCESSION   DATBRK010000340 DATBRK010000000
VERSION     DATBRK010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225076"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VIH70_10050"
     CDS             complement(<1..842)
                     /locus_tag="VIH70_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116743.1"
                     /translation="MAMLFRVPGVSFEELLATPEMASLARAGGAGLLVYQASLPARLG
                     GIGGSSPPKGPFQEFFLPPNASGGVEGVVEEIARIVQASPQQQVLVVVTSLEPSAGMR
                     SAKDELHPIVMAVGAPDELFPETGTAGSLTSDSTRRSGVVIDGDIRPTVSEFIQRPIQ
                     EAEVGAPIEIIPSPPPFELHERYLAQRRMYVPIGTAGAIYLAIAGLGAIVVLALGSRI
                     PRGVRRIFGWGSLSVAALATALLAAGHLPNLTYATVVPFVALVTVFGTLAFAPLEHRD
                     VLLIP"
     gene            complement(932..2203)
                     /gene="murA"
                     /locus_tag="VIH70_10055"
     CDS             complement(932..2203)
                     /gene="murA"
                     /locus_tag="VIH70_10055"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY8116744.1"
                     /translation="MDRLLVTGGTPLDGTVEIAGAKNSALKLMAAVVLSDGRSALRNV
                     PRIQDCITMVEVLEHLGVGCAWKGDALVLNTTEVRSVDTPYELVSRMRASILVLGPLL
                     ARFGNARVAMPGGCNIGSRAIDLHIRGLEKMGARFSSEHGFLVGETPGLRGAVVTLDF
                     PSVGATENVLMAAVAARGTTVIENAAREPELVDLADFLRGMGARIDGVGTPTIEIQGV
                     EGFSPVDHTVIPDRVEAGTYAIAVCSTGGRLVLSGARADHMDLVLSKLADAGADVLAT
                     DEGVAISMGSRARCVDLVTLPYPGFPTDLQPQMMALLASAEGTSIVTENVFEGRFMFV
                     DELNRMGADIRTEGHHAVIRGVERLSSAPVRALDIRAGAAMVIAALTADGVTEIEDMY
                     HVDRGYQDLEAKLTALGAVVRRERERSPALQ"
     gene            complement(2294..2689)
                     /gene="atpC"
                     /locus_tag="VIH70_10060"
     CDS             complement(2294..2689)
                     /gene="atpC"
                     /locus_tag="VIH70_10060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEY8116745.1"
                     /translation="MALDVHLVTPEREVWAGPVQMVIAHGTDGMVGILTGHAPLLIRL
                     SIAPIKIQLEDGSWMVAVVDGGFLHVTSDEDSTRVDVLASYAELSDEIDRDAAQARAE
                     RAQARLAQGDDASARAELAKALARTGLGE"
     gene            complement(2690..4117)
                     /gene="atpD"
                     /locus_tag="VIH70_10065"
     CDS             complement(2690..4117)
                     /gene="atpD"
                     /locus_tag="VIH70_10065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY8116746.1"
                     /translation="MADDALAHGRVARVIGPVVDVEFPPDGLPEIYTALRIDVNLEGK
                     TQTVTCEVAQHIGDGMVRAIALKPTDGMVRGAPVENTGSAISVPVGEGVLGHVYNVLG
                     DPLDTSPDEIHAADRWPIHRQPPPFEELEPKQVMLETGIKVIDLLEPYVQGGKIGMFG
                     GAGVGKTVIIQEMIRRQAQQHGGVSVFAGVGERTREGNDLFLEMTESGVIKNTALVFG
                     QMDEPPGVRLRVALSALTMAEYFRDVERKDVLLFVDNIFRFTQAGSEVSTLLGRMPSA
                     VGYQPTLADEMGDLQERITSTKGRSITSLQAIYVPADDITDPAPHTAFAHLDATTVLS
                     RDIASLGIYPAVDPLDSTSRILDARYVGQEHYDTARRVQEILQRYKDLQDIIAILGVD
                     ELSEEDKVIVARARRIQLFLSQPFFVAEQFTGIPGKYTPRDETIASFRALCDGEYDHL
                     PEQAFLLVGGIEEAVEAAKKMEQAA"
     gene            complement(4121..5008)
                     /locus_tag="VIH70_10070"
     CDS             complement(4121..5008)
                     /locus_tag="VIH70_10070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY8116747.1"
                     /translation="MGAKLRVVRRRIRSVQSTMKITRAMELIAASRIVKAQQRVMEAR
                     PYADLLTAAMEDVARQTGSMVHPLLEERETPSRAGLLVMTSDRGLAGAFNANLLKRAE
                     GLLRRVRSRGLEPVLYLTGKKAIGYFRFRGVTPEASWSGFSEVPRYDAAQEIGARMIE
                     DFGSGRIDELHTVYTDFRSAFTFRATDKRFLPIAPEEVVADASGVPSAEYIFEPEPAE
                     ILDDLLPEYVITKIYFALLESAASENAARQRAMAAATDNADELIKVLTRQANRARQDE
                     ITTEIMEIVGGAEALASKE"
     gene            complement(5222..6751)
                     /gene="atpA"
                     /locus_tag="VIH70_10075"
                     /pseudo
     CDS             complement(5222..6751)
                     /gene="atpA"
                     /locus_tag="VIH70_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            complement(6763..7320)
                     /gene="atpH"
                     /locus_tag="VIH70_10080"
     CDS             complement(6763..7320)
                     /gene="atpH"
                     /locus_tag="VIH70_10080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY8116748.1"
                     /translation="MADKDELVRSYAEAVFRLADAEGELESVERQLFAFAALLEREAR
                     VRDALIDPSLPPENKRRLIAETLGERANPLAVNLLGFLVELGRAREVGRIVDALAEVV
                     AATREHAVAEVRSAVPLNQTRTSELADALSKATGRPVEVRVVLDPSVIGGVVAKVGDE
                     IFDGSVRSRLLEARERLTGTTTRSG"
     gene            complement(7322..7891)
                     /locus_tag="VIH70_10085"
     CDS             complement(7322..7891)
                     /locus_tag="VIH70_10085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY8116749.1"
                     /translation="MLATIFWSLAQAEPEPANDVKDLYPHWEELLVGALAFAILFFFV
                     WKWVLPRISKLLEERRDKIQGDLERAEETRRKAEQELAEYREQLANARQESNGIIEEA
                     RKTAEQLRAEIQARAEREADATVSRAQEEIRAERDRVFQELRGEIGEIAVELAERVVG
                     ASLDAKAHQRLIDEYIDQVGKGTAANGKH"
     gene            complement(7905..8153)
                     /gene="atpE"
                     /locus_tag="VIH70_10090"
     CDS             complement(7905..8153)
                     /gene="atpE"
                     /locus_tag="VIH70_10090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY8116750.1"
                     /translation="MLGLLAQTTGDVGTGLETIGKGLVIGLAAIGPGIGLGVLIGKSV
                     EAMARQPEYAGQVRATMFIGIGLIEALALFGIAFSFII"
     gene            complement(8167..8976)
                     /gene="atpB"
                     /locus_tag="VIH70_10095"
     CDS             complement(8167..8976)
                     /gene="atpB"
                     /locus_tag="VIH70_10095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY8116751.1"
                     /translation="MTGAVGSILPTFLAAFEPPTTKDFVWGCWGPSLRAGGLEFCLNF
                     ITFLVLFGFLLMVSFFYFGLRRPRVVPGKLQSLAEMGISFVNENIAAPMLGPDSSRFL
                     PLLVTFFFAIFFWNIFEVVPGINFSSNSRIAFPLVMAIVAWVTYNAVGIGKHGFLGYL
                     RHVCFPPGAPAGLYILLVPIEFFTQIIIRPITLSVRLWANFVAGHFLLAVFFLGTIAM
                     LEGGFLAIFAPFSFAISVALVGFEIFVSGLQAFIFSILTASYIGGALAEEH"
     gene            complement(8973..9392)
                     /locus_tag="VIH70_10100"
     CDS             complement(8973..9392)
                     /locus_tag="VIH70_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116752.1"
                     /translation="MAPEREMIRRISPFALPVAVVAFILATVVDGTDAGWSAAIAIAV
                     VYLNFVVHGSSLALAATISPTVLYAVGLGGFLVRLGIIVLVIALLQQTEWFSIIAFIA
                     ALIPATIMLLVVEMKLISGRMQADLWADPNARREVTP"
     gene            complement(9382..9618)
                     /locus_tag="VIH70_10105"
     CDS             complement(9382..9618)
                     /locus_tag="VIH70_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY8116753.1"
                     /translation="MAGSGRDGAWAGMGIGWAVVSTLIGGIVVWGGLGYLADRLFGIN
                     PRILTAVGIVLGAGAGIYLVYLRFGKGEGNGGGA"
BASE COUNT         1529 a         3457 c         3230 g         1474 t
ORIGIN      
        1 gggatcagca gcacgtctcg atgctcgagc ggcgcgaacg ccagcgtccc gaacaccgtc
       61 accagcgcga cgaacggaac cacggtcgcg tacgtgagat tgggcaggtg cccggccgcg
      121 agcaatgcgg tcgcgagcgc ggcgaccgag agagaccccc acccgaagat ccgtcggacg
      181 cctcggggaa tccggctccc gagcgcaagc acgacgatcg cgccgagccc agcgatcgcg
      241 aggtagatcg cccccgccgt cccgatcggc acgtacatgc gccgctgtgc caggtagcgc
      301 tcgtggagct cgaacggtgg aggactcggg atgatctcga tcggtgcccc gacctccgcc
      361 tcttggatcg gtcgctggat gaactcggag accgtcggcc ggatgtcgcc atcgatcacg
      421 accccagatc gtcgggtgga atcggacgtc agcgagccgg ccgtccccgt ctccgggaac
      481 aattcgtccg gggcgcccac cgccatgacg atcggatgca actcgtcctt cgccgagcgc
      541 atccccgcgg acggctcgag actcgtcacg acaacgagga cttgctgctg aggcgaggcc
      601 tgcacgatcc gagcgatctc ttccacaacc ccctcgacgc cgcccgaggc attcggagga
      661 aggaagaatt cctggaacgg tcctttcggc ggcgaggatc ccccgatgcc tccgagccgt
      721 gcgggcagcg atgcctggta caccaggagt cctgcgcctc ccgcccgagc gagcgacgcc
      781 atctcggggg tcgcgagcaa ctcctcgaag gagaccccgg gaacacggaa cagcatcgcc
      841 acgggatcgc ctggatcaac ggcggcgccc gcgggcgaac agaggaccaa cgaagcggca
      901 cacgcggcga gcgcgccgag acgcgcgcgg gtcactggag agcgggtgag cgttcgcgct
      961 cgcgacgaac gacggcgccg agcgccgtca gcttggcctc gagatcttgg tacccgcggt
     1021 cgacgtggta catgtcctcg atctcggtga cgccgtcggc ggtcagggcc gcgatcacca
     1081 tcgcggcgcc ggcgcggatg tcgagcgccc gcacgggcgc ggacgagagg cgttcgacgc
     1141 cgcggatcac ggcgtggtga ccctcggtgc ggatgtccgc ccccatccgg ttgagctcgt
     1201 ccacgaacat gaaccggccc tcgaacacgt tctcggtcac gatgctcgtc ccctccgccg
     1261 aggcgaggag cgccatcatc tgcggctgga ggtcggtcgg gaaccccggg tacgggagcg
     1321 tcaccagatc gacacaccgg gcgcgcgaac ccatcgagat cgccacacct tcgtccgtcg
     1381 cgagcacgtc ggccccggcg tcggcgagct tggacaggac caggtccatg tggtcggccc
     1441 gggcgccgga gagcaccagt cgtcctcccg tcgagcacac ggcgatcgcg taggtccccg
     1501 cctccacgcg gtcggggatc acggtgtggt cgaccggcga gaacccctcg acgccctgga
     1561 tctcgatcgt cggggttccg acgccgtcga tccgcgcgcc catcccgcgc aggaagtccg
     1621 cgaggtcgac gagctcgggc tcgcgcgcgg cgttctcgat cacggtggtt ccgcgcgccg
     1681 cgacggcggc catcaggacg ttctcggtcg cgccgacgct ggggaagtcg agggtgacca
     1741 ccgcgccgcg caggcccggc gtctccccca caaggaaccc gtgctcggac gagaaccggg
     1801 cgcccatctt ctcgagcccg cggatgtgga ggtcgatcgc gcgcgacccg atgttgcacc
     1861 cgccgggcat cgcgaccctc gcgttcccga accgcgcgag cagcggcccg agcaccagga
     1921 tcgaggcccg catccggctg acgagctcgt acggcgtgtc gaccgatcgc acctcggtcg
     1981 tgttcaggac cagcgcgtcg cccttccacg cgcagccgac gccgaggtgc tcgagcacct
     2041 cgaccatcgt gatgcagtcc tggatccgcg ggacgttgcg caaggcggat cggccgtcgg
     2101 acaacacgac ggcggccatc agcttgagcg cggagttctt cgcgccggcg atctcgacgg
     2161 ttccgtcgag cggcgtccct ccggtgacga gcaaacgatc catcagcgct ccatcgtagg
     2221 tccgggcgtg ctcgggacca gcacctcggg tcccgctccg ctccgggagg gttgttcccg
     2281 gaaccccgcg aggctactcg ccgaggcccg tgcgggcgag cgctttcgcg agttcggcgc
     2341 gagcgctcgc gtcgtcaccc tgcgcgagcc gtgcctgggc ccgctcggcc cgtgcctgcg
     2401 cggcgtcgcg atcgatctcg tcggacagct cggcgtagga ggcgagcacg tccacccgcg
     2461 tcgagtcctc atcactcgtg acgtggagga agccgccgtc gaccacggca accatccagc
     2521 taccgtcctc gagctggatc ttgatcggcg cgatcgagag ccggatgagg agcggggcgt
     2581 gcccggtcag gatcccgacc atcccatcgg tgccgtgcgc gatcaccatc tgcacgggac
     2641 cggcccagac ctcgcgttcg ggcgtgacga gatggacgtc gagcgccatc tacgcggcct
     2701 gctccatctt cttcgcggcc tcgacggcct cctcgatccc gccgacgagc aggaacgcct
     2761 gctcgggcag gtggtcgtac tcgccgtcgc acagcgcgcg gaacgacgcg atcgtctcgt
     2821 cgcgcggcgt gtacttcccc gggatcccgg tgaactgctc ggccacgaag aacggctgcg
     2881 acaggaacag ttggatccgc cgcgcccgcg ccacgatcac cttgtcctcc tccgagagct
     2941 cgtccacgcc gaggatcgcg atgatgtcct gcaggtcctt gtagcgctgc aggatctcct
     3001 gcacgcgtcg agcggtgtcg tagtgctcct gcccgacgta ccgcgcgtcg aggatccggc
     3061 tggtcgagtc gagcgggtcg acggccgggt agatccccag cgacgcgatg tcgcgcgaga
     3121 gcaccgtggt cgcgtcgagg tgcgcgaacg ccgtgtgcgg cgccgggtcg gtgatgtcgt
     3181 cggccggcac gtagatcgcc tgcagcgacg tgatcgaccg gcccttcgtc gacgtgatcc
     3241 gctcctgcag gtcgcccatc tcgtcggcga gcgtcggctg gtagccgacc gcgctcggca
     3301 tgcgcccgag cagcgtcgag acctcggacc cggcctgcgt gaaccggaag atgttgtcca
     3361 cgaacagcag cacgtccttg cgctcgacgt cgcggaagta ctcggccatc gtgagcgccg
     3421 acagcgcgac ccggagccgg acgcccggcg gctcgtccat ctgcccgaac acgagcgccg
     3481 tgttcttgat gaccccggac tcggtcatct ccaggaacag gtcgttgccc tcgcgcgtgc
     3541 gctcgccgac gccggcgaac accgagacgc cgccgtgctg ttgcgcctgt cggcggatca
     3601 tctcctggat gatcacggtc ttcccgaccc cggcgccgcc gaacatcccg atcttgccgc
     3661 cctgcacgta gggctcgagc aggtcgatga ccttgatacc cgtctcgagc atcacctgct
     3721 tgggctcgag ctcctcgaac ggcggcggct ggcgatggat cggccagcgg tcggccgcgt
     3781 gaatctcgtc cggcgaggtg tcgagcggat cgccgagcac gttgtagacg tgcccgagga
     3841 cgccctcgcc caccgggacg ctgatcgcgg accctgtgtt ctcgacgggc gcgccgcgga
     3901 ccatgccgtc ggtcggcttc agcgcgatcg cccggaccat cccgtcgccg atgtgctggg
     3961 ccacctcgca ggtgaccgtc tgcgtcttcc cttccaggtt cacgtcgata cggagcgcgg
     4021 tgtagatctc gggcagcccg tccgggggga actcgacgtc gaccaccggg ccgatcaccc
     4081 gggccacccg tccgtgcgca agcgcgtcgt cagccatatc tcactccttc gaggccaggg
     4141 cctcggcccc gcccacgatc tccatgatct cggtcgtgat ctcgtcctgg cgggcccggt
     4201 tggcctggcg ggtgagcacc ttgatgagct cgtccgcgtt gtcggtcgcg gccgccatcg
     4261 cccgctgtcg ggcggcgttc tccgaggccg ctgactcgag cagcgcgaag tagatcttcg
     4321 tgatgacgta ctccggtaac aggtcgtcca ggatctcggc gggctcgggt tcgaagatgt
     4381 actcggcgct cggcaccccc gaggcgtcgg ccacgacctc ctcgggcgcg atcggcagga
     4441 accgcttgtc cgttgcccgg aacgtgaacg ccgagcggaa gtccgtgtag accgtgtgca
     4501 gctcgtcgat gcgcccggag ccgaagtcct cgatcatgcg ggccccgatc tcctgcgcgg
     4561 cgtcgtagcg cggcacctcg gagaaccccg accacgacgc ctcgggcgtc acgcctcgga
     4621 accggaagta cccgatcgcc ttcttccccg tcaggtacag gacgggttcg aggccccggg
     4681 atcgcacgcg gcggagcagg ccctcggcgc gcttgagcag gttcgcgttg aacgcgccgg
     4741 ccaggccccg gtcgctggtc atgacgagca ggccggcgcg cgacggggtc tcgcgctcct
     4801 cgagcagtgg gtggaccatc gagcccgtct gccgggccac gtcctccatc gccgcggtga
     4861 gcaggtccgc gtaggggcgg gcctccatca cccgctgctg cgccttgacg atgcgagagg
     4921 ccgcgatgag ttccatcgcg cgggtgatct tcatcgtcga ctgaaccgag cggatccgcc
     4981 gtcgaacgac ccggagcttg gcgcccatcg gctacgaccc cgggacgcgt ggcgaggtgc
     5041 ccccgggctc cgacccctcg gccagggact ccccgccctc cggcggctcc ccctctcgtt
     5101 cgggctcgtc ctcgtcgtcg gccgaggaga ggcggtccca cccgacgtcc ggcttgaccg
     5161 ggtccctcgg tgcgccccga cccacgccgg cctcgctccc ggcgcccgca gccgacggca
     5221 cgaagctctg cgtgaagtcc tcgagcgcct ccttgagcgt gtcccctgcc gcgtccgaga
     5281 gctcgcccgt gtcccggacc tggtccagga cgttggtccg agcggccagg tgctcgatga
     5341 actcgtcgac gaaccgtttg gcgtcctcga ccggcagggc gtccagatac ccgcccgtgc
     5401 cgacccagat cgcgacgacc tgccgctcga ccggcacggg ccggtactgc ggctgcttga
     5461 ggatctctac gacgcgggca ccgcgcgcga gctgcttctg cgactccttg tccagctcgg
     5521 agccgaactg cgcgaacgcc tcgagcgccc ggtactgcgc catcgcgatc cgcaacgtcc
     5581 cggcgatctt cctcatcgcc ttgatctgcg cgttcccccc cacgcgcgag accgacgtgc
     5641 cgacgttgat cgcggggcga acccccgcga agaacaggtc gggctccagg tagatctggc
     5701 cgtccgtgat cgagatgacg ttggtcggga tgtacgcgga gatgtcgttg cccttggtct
     5761 cgacgatcgg cagcgccgtc agcgaacccg cgccgagccg gtccgagagc ttggcggcgc
     5821 gctcgaggag ccgggagtgg aggtagaaga cgtcgcccgg gtaggcctcg cgacccggcg
     5881 gccgtcggag caggagggag agcgtccggt aggccgtggc ctgcttggac aggtcgtcgt
     5941 agaccgcgag cgcgtgcagg ccctcgtaca tccagtaggc gcccagggcc gccccggagt
     6001 acggggcgag gtactggaag ggcgtcgggt ccgacgcagc ggcgttcacg atcaccgtgt
     6061 agtcgagcgc gccgttgtca ctcagcgtct cgaccacctc ggccacggtc gaggccttct
     6121 ggcccaccgc gacgtagatg cagcggacct gctgcttggg gtcgccgctc gcccagttgt
     6181 cgcgctgggc gatgatcgcg tcgatcgcca cggcggtctt gccggtctga cgatcgccga
     6241 tgatcagctc ccgctgaccg cgtccgatcg gcgtcatcgc gtcgatcgcc gtgatgccgg
     6301 tctgcagcgg ctccttcacg ggctggcggt cgatgacgtt gggggcctgg agctccagga
     6361 ccctggtgtc cccggcctgg atggggccct tcccgtccaa gggggcgccc aggggatcga
     6421 tcacgcgacc gaggaacccg tcgcccacgg ggatcgacag gatcttgccg gtctgcttga
     6481 cgaggtcgcc ctcctcgatc tgggaggact cgcccaggat gatgcagccg atctcgtcct
     6541 cgtccaggtt gaacgcgagg ccgagcacgc cgccggggaa ctcgagcagc tcgttcgcca
     6601 tcgcacgggg cagccccttc acgcgcgcga tgccgtcccc cgcctcgacc acacgaccga
     6661 cctcctcgcg ctcgacggag ggcgtgaagc tctcgacgtt cttgcggagc gcctccgcga
     6721 tcttcttcgg atcgaagttc agctcgtcca ctgccatcgg gttcacccgc tccttgtggt
     6781 cgttccggtc agccgctccc gcgcctccag caggcgcgag cgcacggacc cgtcgaagat
     6841 ctcgtcgccg accttggcca cgacgccgcc gatcacgctc gggtcgagga cgaccctgac
     6901 ctcgaccgga cgtcccgtgg ccttggagag agcatcggcc agctcgctgg tacgggtctg
     6961 gttgagcggg acagccgagc ggacctcggc gaccgcgtgc tctcgcgtcg ccgcgacgac
     7021 ctcggcgagc gcatcgacga tccggccgac ctcccgggcg cgtccgagtt cgacgaggaa
     7081 cccaagcagg ttgaccgcca gggggttggc gcgctcgccg agcgtctcgg cgatcagccg
     7141 ccgcttgttc tccggcggca gcgacgggtc gatcagcgcg tcccgcacgc gcgcctcgcg
     7201 ctcgagcagc gcggcgaacg cgaacagctg gcgctcgacc gactcgagct cgccctcggc
     7261 atcggccagg cggaacaccg cctccgcgta gctgcgaacg agctcgtcct tgtccgccac
     7321 gtcaatgctt cccgttcgcc gcggttccct tgcctacctg atcgatgtac tcgtcgatca
     7381 gccgctgatg cgccttggca tccaacgacg cgccgacgac gcgctccgcc agctccacgg
     7441 cgatctcacc gatctccccc cggagctcct ggaacactcg gtcgcgctcg gcgcggatct
     7501 cctcctgggc cctcgagacc gtggcgtcgg cctcgcgctc ggcccgcgcc tggatctcgg
     7561 cgcgaagctg ctcggccgtc ttcctcgcct cctcgatgat gccgttcgac tcctgccggg
     7621 cgttggcgag ctgctcccgg tactcggcga gctcctgttc ggccttgcgg cgcgtctcct
     7681 ccgctcgttc gagatcaccc tggatcttgt cgcggcgctc ctcgaggagc ttgctgatgc
     7741 gagggagcac ccatttccac acgaagaaga acaggatcgc gaacgcgagc gccccgacga
     7801 gcaactcttc ccagtgcggg tacaggtcct tgacgtcgtt ggccggctcg ggctcggcct
     7861 gtgcgaggga ccagaagatc gtggccagca cgtcggctcc ctccctagat gatgaacgag
     7921 aacgcgatgc cgaacagcgc gagcgcctcg atcagcccga tgccgatgaa catcgtcgcg
     7981 cgcacctgcc ccgcatactc gggctgacgg gccatcgcct cgaccgattt gccgatcagg
     8041 accccgagcc cgatgccggg cccgatcgcg gccaggccga tgacgaggcc cttgccgatg
     8101 gtctccagac cggtgccgac gtccccggtc gtctgagcga gcaaccccaa catgctgcct
     8161 ccttgtctag tgctcttcgg ccagcgcccc gccgatgtag gacgcggtga ggatcgagaa
     8221 gatgaatgcc tgcaagcccg acacgaagat ctcgaacccc acgagcgcga ccgagatggc
     8281 gaacgagaac ggagcgaaga tcgcgaggaa gcccccctcg agcatcgcga tcgtgcccag
     8341 gaagaacacc gcgagcagga agtgcccggc cacgaagttg gcccagagac ggaccgacag
     8401 ggtgatcggc cggatgatga tctgtgtgaa gaactcgatc gggaccaaca ggatgtacag
     8461 gcccgccggt gcgcccggcg ggaagcacac gtgccgcagg tagccgagga acccgtgttt
     8521 gccgatcccg accgcgttgt aggtgaccca ggcgacgatc gccatcacga gcggaaacgc
     8581 gatccggctg ttggacgaga agttgatacc gggaacgacc tcgaagatgt tccagaagaa
     8641 gatcgcgaag aaaaacgtca ccagaagggg caggaaccgc gagctgtcgg ggccgagcat
     8701 cggcgcggcg atgttctcgt tcacgaacga gatccccatc tccgcgaggc tctggagctt
     8761 ccccggcacc acccgcggcc ttcgcagccc gaagtagaag aacgacacca tgagcaggaa
     8821 cccgaacaag acgaggaacg tgatgaagtt gaggcagaac tcgaggccgc ccgcgcgcag
     8881 cgacgggccc cagcaccccc agacgaagtc cttcgtcgtg ggcggctcga acgcggccag
     8941 aaacgtgggc aggatcgacc cgacggcccc cgtcacggcg tcacctctcg acgggcgttc
     9001 gggtccgccc acaggtccgc ctgcatccga ccggagatca gcttcatctc gactacgagc
     9061 agcatgatcg tggccgggat cagggccgcg atgaaggcga tgatcgagaa ccactcggtc
     9121 tgttggagca gcgcgatgac caggacgatg atgcccagtc gcacgaggaa ccctccgagt
     9181 ccgaccgcgt agagcaccgt cggcgagatc gtggcggcga gcgccagcga ggatccgtgc
     9241 acgacgaagt tgaggtagac cacggcgatc gcgatggcgg cggaccatcc cgcgtcggtg
     9301 ccgtcgacca cggttgcgag gatgaacgcg acgacggcga cgggaagcgc gaacggcgag
     9361 atgcgccgga tcatctctcg ctcaggcgcc accgccgttc ccttcccctt tgccgaatcg
     9421 caggtacacg aggtagatcc ccgctccggc ccccagcacg atccccaccg cggtgaggat
     9481 gcgcgggttg atcccgaaca gccggtcggc caggtacccg aggccccccc acacgacgat
     9541 ccccccgatc agcgtcgaga cgaccgccca cccgatcccc atgccggccc aagcgccgtc
     9601 gcgaccggaa cccgccatgg gcgcggcagt atatccgcgc tccatctgac gcgtcaaacc
     9661 ttcaaagtcc cgtttccgca ggtagatggc
//
LOCUS       DATBRK010000341        16749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2225162, whole genome shotgun sequence.
ACCESSION   DATBRK010000341 DATBRK010000000
VERSION     DATBRK010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16749
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225162"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VIH70_10110"
     CDS             <1..673
                     /locus_tag="VIH70_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8116754.1"
                     /translation="RRHLRGCAACREERVKLEEGVSALSRAVHDRQPPPELRDRVLGT
                     LGEEWEESVPLPATPVPSRGAGRERSPWRAFAVAAALILVVGSIAFGFAQGHRASLAT
                     TDAQSYRNLLVALGGKEFRIGELRPAEGSSMHGQVLIYDGDPSGDWSSWALVFAKVPG
                     YSGSATATLLSPEGDSRALRPIEFHDGDGETWLVTYADLTRFDSLTVTSPTGQVLATA
                     TLEHA"
     gene            965..1378
                     /locus_tag="VIH70_10115"
     CDS             965..1378
                     /locus_tag="VIH70_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8116755.1"
                     /translation="MAVLMSACSSSSGSGSSSGGEGDEGSSGTITLNGQTANDHGTKD
                     VSGGGKEEVEVDSFYFNPTVFTAHAGDSLTLTLSNESSTLHNFSLPEQNIDMDIPADD
                     SITVTVVFPDSGTQVFFCKYHQSSGMAGGLKVETG"
     gene            complement(2022..4298)
                     /locus_tag="VIH70_10120"
     CDS             complement(2022..4298)
                     /locus_tag="VIH70_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8116756.1"
                     /translation="MPDSRSSTAARLAGHRIRRLLLGRPVPTSGERREHLGKPAGLAV
                     LSGDALSSVAYATEAMIRVLVPVAGIAAFSLVVPIAWVIIALLVTLVFSYRQTIKAYP
                     TAGGAYVVTRDNFPSPVPQIAATGLLLDYVLTAAVSASAGVAAVYSAFPAVYPYRVVS
                     AVGIIWLIAWINLRGIRVTGRVFVPPIYAFVAAILGLIVVGVVRLGTGAIQPLPPPAS
                     IRGLTGSVGLYVMMHAYASGTTALTGVEAISNGVPLFRPIEWRNARTVLTWMGAILAV
                     LFGGTVFLASRLHPVPTPDETLLSEIARAVFGTGALGRAAYLFVQITTTSILIFAAET
                     SFSDFPRLSSFAARDGLLPAMFTRRGWRLALSAGILALACLASLITIVLGANIERLIP
                     LFAVGAFSAFTFSQAGMTVHHLRLREPGWRHSLAINGAGAVLSGSALVVILVAKFTQG
                     AWVAFVVIPLGAVALTSIRRHYTRIERRLASARASTSQVTCLEVVVVVWELDESLERA
                     VRYAWSMSGTGGLHAVHPGPPESALAAVFSSRYGLELRCIGARRAGQRIRRLVRGLKR
                     GHADRIVLVVIPEKVEPLSKQRPRSVARVRHLRRAVFRERGVAVAIVPTLPEDDSLVS
                     GRSDRHVAIVVVDGTDIVADRAMAVARLVTGEDPRLVHFDIDREETERLRASWKRDEP
                     ELELEIEPAPIRELSDSIVSFVRSTRRGHPGFVTVVRGDVVPHWWQRFLHADETREAK
                     AALLHEPGTAFIAVPYHL"
     gene            complement(4498..5940)
                     /locus_tag="VIH70_10125"
     CDS             complement(4498..5940)
                     /locus_tag="VIH70_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEY8116757.1"
                     /translation="MAERGPEGLGERERTGAEDEREWRSTALPPFQDKIGAWLDDRLR
                     LSSFTRGALGKVFPDHWSFMLGEIALYSFVVLVLTGIFLTFFYEPSSAPTTYGGSYNP
                     LRGLEVSNAYASTLRLSFDVRAGLVMRQTHHWAAVIFLGAIVVHLCRVFFTGAFRRPR
                     ELNWIIGVTMLLLAMFNGFSGYSLPDDLLSGTGLRIMYSVILSVPLVGPWMAFLLFGG
                     EFPSGQILGRLFVLHIFLVPVLLGGLLAAHLALIWHQKHTQFREPGRTERNVVGSRLW
                     PTYALKSVGLFLIIAGVLTALGGLVQINPVWLYGPFEPAQVSSPAQPDWYLGWLEGAL
                     RLFPNWEIRAFGFEIPEPFFPAVLLPGITFGLLYAWPFLERWVTKDRSEHHLLDRPRD
                     RPWRTALGAATLTFYGVLFAAASNDLLAHWFGLDIETVTTVFRALALVLPPIVGYVTL
                     RVCIGLRDAGDVRLLHMPISALRIPRARRR"
     gene            complement(5933..6778)
                     /locus_tag="VIH70_10130"
     CDS             complement(5933..6778)
                     /locus_tag="VIH70_10130"
                     /inference="COORDINATES: protein motif:HMM:NF039285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8116758.1"
                     /translation="MRRSGQGLAVGVAFGASVLASLGLAVVYVLGGHPQIEGALLLVA
                     LGGIGVALVLWAKRFLPVGDDEEAREPLPSRPEERAAADETFEAGAELIGRRRVLVRM
                     LAAAFGALGVALLFPIRSLGRAPGSTLFHTKWRAGIRAVRENGVPIRASEMVAGTVVT
                     VFPEGDIGVADSQTLLIGLEPGTNRPLPGREQWAPEDVVGYSKICTHVGCPVGLYQPA
                     EHLLFCPCHQSAFDVLNGAMPVAGPAARPLPQLPLSTDAEGYLIAQGDFPEPVGPGFW
                     DRSRG"
     gene            complement(6784..7599)
                     /locus_tag="VIH70_10135"
     CDS             complement(6784..7599)
                     /locus_tag="VIH70_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8116759.1"
                     /translation="MRRWIAVAVPVAAALALAGAACSYFTEEEASPYRPPGITSEVAT
                     LGAGRTLYERDCAWCHGSAGQGTAFGPDLSGPLDGGAYTHFMLVTGRMPLEDPDERAV
                     AGPSRYDAAQTDAIVAYVETFGGTGPGVPMPDPSAGDLTQGAELYLQNCAACHSATGA
                     GGALTSGQVVPGLLHTSAVAVAEAMLVGPGCPNTSRTCGAGEGAMPRFDLSDREMNSI
                     VRYVLYLQRPQDEGGAAIGRVGPVAEGAVALGVGLIVLLLVARWIGTRTGERG"
     gene            complement(7599..8450)
                     /locus_tag="VIH70_10140"
     CDS             complement(7599..8450)
                     /locus_tag="VIH70_10140"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY8116760.1"
                     /translation="MLAHATVAPADLWGAWPFDPLVVFALVSAIVAYARGYRRWRRRS
                     AAAAACFACGILAVAVALLTPLDAAAEALFSAHMTQHLILVVVAAPLLVAGRPVTTVM
                     VGLPAGARRTASRLRRAVAASGLSRALRTPVVAWTVFTICLWAWHTPLLYTVALENGA
                     VHAIEHVSFLLTSMLAWSVALSRRSVTPLGVLGRALFLVATALQGAVLGALLLFARAP
                     LYPVHGRGPWLWGLTPLEDQQLAGALMWIPPSVIYLAVAAGILVAAFKTPSIQARPER
                     VRAGMVT"
     gene            complement(8450..8872)
                     /locus_tag="VIH70_10145"
     CDS             complement(8450..8872)
                     /locus_tag="VIH70_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116761.1"
                     /translation="MANARSAPDAPAGSRVPPSLLSWLGVLTGPIAWMIQLLASWALA
                     EAVACAPATQATGDVLGIGVNAFLAIVNAVLLGLTALSGVGSYIELRRFRGQSDPTPG
                     DRATWLATAGVMTSILFTVLIAVSFVPAGLIAGCQEAG"
     gene            complement(8872..9504)
                     /locus_tag="VIH70_10150"
     CDS             complement(8872..9504)
                     /locus_tag="VIH70_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8116762.1"
                     /translation="MTVATHELEPTERLDEVLPSTANGSASFAWWGMVWLIATEAALF
                     ATIIVSYWYLRFRSAPVWPPDGIAKPELALPLIMSAFLWSSSVPVHMADRAIRRGDQR
                     TLRIGLLIGWILGAAFLALLLAVEYPGVLREFTPRTNAYGSLFFTLTGLHGLHVAVGL
                     SFSAWVQVRAWWGAFDERRHVTVQNFAMYWHFVDVVWFFVLLTIYVSPHL"
     gene            complement(9501..11384)
                     /gene="ctaD"
                     /locus_tag="VIH70_10155"
     CDS             complement(9501..11384)
                     /gene="ctaD"
                     /locus_tag="VIH70_10155"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY8116763.1"
                     /translation="MASYGERLEPLWREGSGVASWVGTVDHKRIGLRYLYTGFAFFVV
                     AGLEAVLIRVQLARPGLSVLSPQAYNELFSMHGVTMMFLFATPVLSGFGNYFVPLMIG
                     ARDMAFPRLNAFGYWVFLGAGIFLNSAFIVGQAPDDGWFNYVPLANANFTPGLAIDFY
                     ALGLIFIGVSTTAGAINFIVTILKLRAPGMTLNRIPIFVWGELAFALTVVFALPALTI
                     AAVFLELERRFGFHFYDTTGGGDPVLWQHLFWFFGHPEVYLVVLPALGLASAIIPTFS
                     RRTMIGYTYIVLAELFIFVISFGVWVHHMFAVGLPTIAMGFISAASFMVVIPSGTQIF
                     AWLATMVSGRLVLSTPMLFVLGFILLFVIGGLSGAMFSAVPFDQATTDSYFVVAHFHY
                     VLAGGAVFPMFAGLYHWGPKILGRKLDERLGKISFWLMFVGFNLTFFPMHIAGLLGQP
                     RRTYTYHGGLGWDLPNLLSTIGVFVMGAGIVVTFWNWLRSERRGEPAGADPWGGETLE
                     WTTSSPPPEYNFETIPTVRSREPAWDQPELHGGAQPPERGGRPLTTGHTTLSTSVADA
                     EPQAIVRMPHASPWPLFLTMALMLTFYGLLLSSWGVAVSGAASSVGALMGWLWPRGET
                     QET"
     gene            complement(11384..12295)
                     /gene="coxB"
                     /locus_tag="VIH70_10160"
     CDS             complement(11384..12295)
                     /gene="coxB"
                     /locus_tag="VIH70_10160"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY8116764.1"
                     /translation="MLSPQGDSARRINGLWWLMFWISLVVFVVVAGLMAAAIVRRRRR
                     GLEVDASEPRWGDPFVVIAGVVVPAVILTSVLVVSARDLVAMTDTRDAQLTIQVVGHM
                     WWWEVRYPDGVETANEIHIPVGETVRIEVSSVDVIHSFWVPELAPKIDMIPGRTNVLD
                     VRADRSGTYRGQCAEFCGLQHANMAFFVVAEPRAEFDRWLRNESLPAGIPSPRATAGL
                     RIFLESSCVGCHTIRGTEASGTLGPDLTHVAARHTLAAGTIANTRDDLAAWISDPQSV
                     KPGAVMPPTELSPQELSAVVEYLEGLT"
     gene            complement(12918..13502)
                     /locus_tag="VIH70_10165"
     CDS             complement(12918..13502)
                     /locus_tag="VIH70_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116765.1"
                     /translation="MVANDVPHPLCETLTVARFLGGRGNRVLVFDYRDRGNSDASDAP
                     GRLDQDVAGAVDELRSRGVARVVLLGSYAGVAAAVVAATEIDPPVDGVIGISPAAVRG
                     QWVEGPFGPIGAFQAAPRLRVPALYITVRSDRYVSLGEVRRLYRLTASPDKDLVVIPS
                     GSGGFDSIDFSSYEGRVRDAILSFVRHVAGVQPS"
     gene            13696..13848
                     /locus_tag="VIH70_10170"
     CDS             13696..13848
                     /locus_tag="VIH70_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116766.1"
                     /translation="MDAELQAARTRAATEAMTHHRTDPVGSFGTGSAPIVPPAMDRVE
                     EFTASP"
     gene            complement(13845..14462)
                     /locus_tag="VIH70_10175"
     CDS             complement(13845..14462)
                     /locus_tag="VIH70_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8116767.1"
                     /translation="MWFSASDGVRLYGIESGAGKTAVVLAHEGGADLCEWVPYIKTLN
                     RAGIRAFAFDFRGYGNSYSPDTARLALGHDLAGAVAQVRADGAKRVFLMGASMGGAAV
                     VQNSAGIRVDGLISLSGTRLLSGYGINDPAGLRSLSAPFLYVGTRDDPRAPQREALSV
                     YKRVGSSDTQIVLYPGSAHGTTLVDVPPHGDRTRALILRWIETRS"
     gene            14800..15369
                     /locus_tag="VIH70_10180"
     CDS             14800..15369
                     /locus_tag="VIH70_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY8116768.1"
                     /translation="MSLRHALLGLLAGSPMSGYELNRKFERSLLNVWPAGHSQIYPEL
                     ARLSEAGLIRQSGSGARGKKIYEITPEGLKEVRAWLRTEPDHASRNPAFLRVFFLWLM
                     EPEQAIAFLDNEALVHEAKLIEFQGIESRRTRGDDEAWAFGLALEWGIRYEQAILEWN
                     RWARQQIAARAAPTSGVSPSPSPSTGPRS"
     gene            15612..16004
                     /locus_tag="VIH70_10185"
     CDS             15612..16004
                     /locus_tag="VIH70_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY8116769.1"
                     /translation="MIQIESPALSEGGVIPTRFTCDGENVSPPLRWSGVPEDATELRI
                     SLTDRDAPRGTFTHWLVTGVDRATSKVEEGAAPVGGTEGRNDFGDTGYGGPCPPAGAP
                     HRYVFTVEALDAAGKVVGSGQLTATYGR"
     gene            16215..16637
                     /locus_tag="VIH70_10190"
     CDS             16215..16637
                     /locus_tag="VIH70_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8116770.1"
                     /translation="MGKPRKPNSARHPPVASDSHAEIADWARRVMPDLHPIVKRLDEL
                     IRETIPGLQYAVKWKRAYYGLPELGWIIEMVAYDVSVNVVFFGGAEFDPPPPLGTPER
                     TRYVKLKTLVEAQEPEMHKWIEQAGRVPGWKLNVDADA"
BASE COUNT         2974 a         5617 c         5822 g         2336 t
ORIGIN      
        1 ccggcggcac ctccgtgggt gcgccgcctg ccgtgaggag cgggtgaagc tcgaagaggg
       61 cgtctcggcc ctgtcccggg ccgtccacga tcgacagccg ccgcccgagc ttcgcgaccg
      121 cgtcctgggc acgctcggcg aggagtggga ggagagcgtg cctttgccgg cgacgcccgt
      181 cccctcccgt ggggccggcc gcgagcgctc gccctggcga gccttcgccg tcgccgccgc
      241 gctgatcctg gtggtcggct cgatcgcctt cggcttcgcc caggggcacc gcgcgtcctt
      301 ggccacgacc gacgcgcaga gctaccggaa cctgctcgtg gcgctcggtg ggaaggagtt
      361 caggatcggt gagctccgac cagccgaggg atcctcgatg cacgggcagg tgctgatcta
      421 cgacggcgac ccgtccggcg attggtcctc gtgggcgctc gtgttcgcga aggtgcccgg
      481 ctactcgggt tccgcgacgg cgacgttgct ctcgcccgag ggcgattcgc gggctcttcg
      541 accgatcgag ttccacgatg gcgacgggga gacctggttg gtcacctacg ccgacctgac
      601 tcgattcgac agcctcacgg tcacctcccc gaccgggcaa gtgttggcga ccgcgacgct
      661 cgaacacgcc tgatcccgcc ggggggttcc gatgcgtcga tccatcccct ccgttcgcct
      721 cactggcagc ggcggccggg gagctcgagc ttctcgtcag gcgcagttca ccgagcagag
      781 ttttggagag tggccgaagg aagagtctcg gaagggcttc tggcgagacc tggaaacctc
      841 ggacatatga accccgaagc cctctcgctc gtagacccct caggtgtgga cgcacggtcg
      901 aacgaaaggg gtggccatga gggatcggaa caggctgcag cttgccgtcg ccggattcat
      961 gacgatggcg gtgctgatgt ccgcctgctc atcgagttcg ggatccggct cctcctcggg
     1021 aggtgaaggg gacgagggct cgtccggaac catcacactg aacggccaaa ccgcgaatga
     1081 ccacggcacg aaggatgtgt cgggcggcgg caaggaagag gtggaggtcg acagcttcta
     1141 cttcaacccg accgtcttca cggcccacgc aggggacagt ctcacgctca ccctgagcaa
     1201 cgaatcctcg accctgcaca acttcagcct tcccgagcag aacatcgaca tggacatccc
     1261 ggccgatgat tcgatcaccg tcacggtggt cttccccgat tcggggacgc aggtcttctt
     1321 ctgcaagtac caccagtcaa gcggcatggc cgggggtctg aaggtcgaga cgggttaggg
     1381 acggcggcag gtcagcgggt tcggcacggc ggataccgcg gacgacggat ccatgcgccg
     1441 cggcggcgcc gccagacttc gccgatcgga ccgtttgccc gactgagttc gtgccggtga
     1501 cggccgcgca gcggggagat gcgattccag cctaggtgct cttgaacccc tcatcagcga
     1561 cggcgcggct cctcggtcaa cgctctgtgc ggcgccacgc ggcggtctct gcgcgggtgc
     1621 ggtatgaacg agtgatgagg gagtggccgg ctcgcgccgc cgtcgcggcg gtcgcggccg
     1681 tcgcggcgtt cctgagtctg acggcggcgg tcgcctgtga gcgcaccccg gccgccgaac
     1741 agccaccctc acccgtcgca tcgggaccag gtcggctcac agcagcgtca gcgacgcgca
     1801 cctgtccatc acgccgaggg aaggctcgaa gagggccgag ccgggggccg ggatctccgt
     1861 gaccgtttcg ggtggcacga tcaccagcgt gaccgcgatg gcctcgggtg acgcggacgc
     1921 agtgcatacc tatcggttcc tatcgctccc gttccttcag ggacgaaacg gagatcgggc
     1981 gcggatccca cggtcacttc gagcacgaac gtgccgtgcc actacaggtg atagggaacg
     2041 gcgatgaagg ctgtgccagg ctcatgaaga agtgccgctt tggcctccct agtctcgtcg
     2101 gcgtggagga agcgctgcca ccagtgaggt actacgtcgc cgcggacgac tgtgacgaag
     2161 ccggggtgtc ctcgccgggt cgatcgcacg aacgagacga tggagtccga cagctcgcgg
     2221 atcggcgcgg gctcgatctc gagctccagt tccggctcgt cgcgcttcca cgaagccctc
     2281 agtcgctccg tctcctcacg atcgatgtcg aagtgcacca accgcgggtc ctctccggtg
     2341 accagccgag caacggccat ggcccgatct gcgacgatgt cggttccgtc caccacgacg
     2401 atggcgacgt gacgatccga gcggcccgag accaatgagt cgtcctccgg cagcgtcgga
     2461 acgatcgcca ccgccacgcc gcgctctcga aagaccgctc ggcgcagatg acggacccga
     2521 gcgacgctgc gcggacgctg cttggatagg ggctcaacct tctcggggat cacgaccagg
     2581 acgatgcgat cggcgtgccc tcgtttcagc ccacggacaa gccgtcgaat ccgttgcccg
     2641 gcgcgccgtg ccccgataca tcgaagctcc aatccgtatc gtgagctgaa caccgcggca
     2701 agtgccgatt cgggtggccc gggatgcacg gcatgcagcc ccccggtccc cgacatactc
     2761 caggcatagc ggacggctcg ctccagcgac tcgtcgagtt cccagacgac gacgacgacc
     2821 tccagacagg tcacctggga cgtcgacgcc cgtgccgacg cgagcctgcg ctcgatgcgc
     2881 gtgtagtgac gtctgatcga ggtcagcgcc accgccccga gggggatcac cacgaatgcg
     2941 acccacgcgc cctgtgtgaa ctttgccacc aggatcacca ccagggccga tccgctcaag
     3001 acggcgccgg cgccgttgat cgccaggctg tgtcgccatc cgggttcgcg cagccggaga
     3061 tggtgcacgg tcatccccgc ctgcgagaag gtgaacgcgc tgaaggcccc caccgcgaac
     3121 agggggatca ggcgttcgat gttcgccccc agcacgatcg tgatcagcga ggcgaggcag
     3181 gcgagtgcga ggattccggc ggacagggcc aggcgccatc cccttcgggt gaacatcgcc
     3241 ggaaggaggc cgtccctcgc ggcgaagctg gacagacggg ggaaatcaga gaaggaggtc
     3301 tcggccgcga agatcaggat gctggtagtc gtgatctgga cgaagaggta ggcggccctt
     3361 cccagcgcac cggtcccgaa cacggctcgg gcgatctcgc tcaacagcgt ctcgtccggg
     3421 gtggggaccg gatggagccg agaggccagg aagacggtgc ccccgaacag cacggcgagg
     3481 atcgccccca tccacgtgag cacggtcctc gcgttccgcc attcgatcgg gcggaagagc
     3541 ggcacgccgt tcgagatggc ctccaccccg gtgagcgcgg tcgttccgct cgcgtacgca
     3601 tgcatcatca cgtacaggcc cacggatccc gtcagtccgc ggatggacgc gggcggaggc
     3661 aggggttgga tcgccccggt gcctagccgg accacgccca cgacgatgag accgagaatc
     3721 gccgcgacga acgcgtagat cggcgggacg aacacccgac cggtcacccg gatgccgcgg
     3781 agattgatcc atgcgatgag ccaaatgatg ccgaccgccg agacgacgcg gtacgggtat
     3841 accgccggga acgccgaata gaccgccgcg acgcccgccg aagcgctgac ggcggcggtc
     3901 agcacgtagt cgaggaggag cccggtggcc gcgatctgcg gcacgggaga ggggaagttg
     3961 tcccgggtga cgacgtacgc gcctcccgca gtgggatagg ccttgatcgt ctgccggtac
     4021 gagaagacca gcgtcacgag caacgcgatg atcacccagg cgatgggaac cacgagggag
     4081 aacgccgcga tcccggcgac cgggaccagg acccggatca tggcttcggt ggcgtaggcg
     4141 accgagctca gcgcgtcgcc cgagagcacg gccagcccgg cgggcttgcc cagatgctct
     4201 cttcgctccc ccgacgtcgg caccggccgg ccgagcagga gccggcgtat ccggtgaccc
     4261 gccagacgag cggcggtgga gctccggctg tcaggcatgg cggcggacgc ggatcacacg
     4321 gcggcatggg gatccagagg aaggtgatgc acggcgaggg cggtgcgggc gatcgcggtt
     4381 cggcaccgag gacagagcgt accgggatgg ctccgcgcgg cgcgtcacct cgcatggaag
     4441 ccccggggga gtggtccgat cgcgtctcga tcggctcggg ctcagccgcg ccacgatcta
     4501 cctgcgacgg gctcggggga ttcgcaacgc cgagatcggc atgtgcagga gccggacgtc
     4561 gcccgcatcg cggagtccga tacacacgcg caacgtcacg taccccacga tcggtggcag
     4621 gaccagcgcg agcgcacgga agaccgtggt cacggtctcg atgtccaggc cgaaccagtg
     4681 ggcgagcagg tcgttgctcg ccgccgcgaa cagcacgccg tagaacgtta gggtggccgc
     4741 gccgagcgcc gtccgccacg gtcggtcgcg cggtcggtcc agcaggtggt gctcggaccg
     4801 gtccttggtg acccagcgct cgaggaaggg ccaggcgtac agcaggccga aggtgatccc
     4861 cggaagcagc accgccggga agaacggctc ggggatctcg aagccgaacg cccggatctc
     4921 ccagttgggg aacagccgga gcgcgccctc gagccaaccg aggtaccagt ccggctgcgc
     4981 cggagagctc acctgcgcgg gctcgaacgg cccgtagagc cagacggggt tgatctggac
     5041 caggcccccg agcgcggtca ggacgccggc gatgatcagg aacaggccga cggacttcag
     5101 ggcgtacgtg ggccacaggc gcgagcccac gacgttgcgc tcggtgcgtc ccggctcgcg
     5161 gaactgcgtg tgcttctgat gccagatcag cgcgaggtgc gcagcgagca ggcccccgag
     5221 cagcacgggc acgaggaaga tgtggagcac gaacagacgg ccgaggatct ggccggaggg
     5281 gaactcgccg ccgaacagca ggaaggccat ccacggcccg acgagcggca ccgacaggat
     5341 gaccgagtac atgatgcgta ggcccgtgcc cgacaggagg tcgtcgggca gcgagtagcc
     5401 ggagaatcca ttgaacatcg cgagcaacaa catcgtgacc ccgatgatcc agttgagctc
     5461 gcgcggcctt cggaacgcgc cggtgaagaa gacccggcaa aggtggacga cgatggcgcc
     5521 gaggaagatc accgccgccc aatggtgggt ctgcctcatc accagcccgg cccgcacgtc
     5581 gaagctgaga cgcagcgtcg acgcgtaggc gttggagacc tccagaccgc ggagggggtt
     5641 gtacgagccc ccgtacgtcg tgggcgccga gctcggttcg tagaagaacg tcaggaagat
     5701 cccggtcagc acgagcacga cgaagctgta gagggcgatc tccccgagca tgaacgacca
     5761 gtggtcgggg aagactttgc cgagcgcgcc gcgggtgaag ctcgacagcc ggagccggtc
     5821 gtcgagccac gctccgatct tgtcctggaa cggtgggagc gcggtcgacc gccactcgcg
     5881 ttcgtcctcg gcgcccgttc gctcgcgctc accgagcccc tcgggcccgc gctcagccac
     5941 gggaccggtc ccagaacccg gggcccaccg gctcggggaa gtcgccctgg gcgatcaggt
     6001 agccctcggc gtcggtcgac agcggcagct gcggcaacgg ccgagccgcg gggccggcca
     6061 ccggcatcgc gccgttcagg acgtcgaagg ccgactggtg gcaggggcag aagagcaggt
     6121 gttcggccgg ctggtacagg ccgacgggac agccgacgtg cgtgcagatc ttcgagtagc
     6181 cgacgacgtc ctcgggcgcc cactgctcgc gcccgggcag cggccggttg gtgccgggct
     6241 cgagtccgat cagcagggtc tgcgagtcgg ccacgccgat gtcgccctcg gggaagaccg
     6301 tcacgacggt gccggcgacc atctcgctgg cccgaatggg gacgccgttc tctcgaacgg
     6361 ccctgatgcc ggctcgccac ttcgtgtgga acagggtcga gcctggggcg cgtcccagcg
     6421 atcggatagg gaacagcagc gcgacgccca gtgctccaaa cgcggcggcg agcatgcgga
     6481 cgagcacgcg acggcgcccg atgagctcgg cacccgcctc gaacgtctca tcggcggccg
     6541 cgcgttcctc cggcctcgac gggagcggct cgcgtgcctc ctcgtcgtcg ccgacgggaa
     6601 ggaaccgctt cgcccacagg acgagcgcca cgccgatgcc ccccagcgcc acgagcagca
     6661 gcgctccctc gatctgtgga tgcccgccga ggacgtacac cacggcgagg ccgagcgagg
     6721 cgaggacgct cgctccgaac gctaccccga cggcgaggcc ctgcccggaa cgccgcacgg
     6781 agctcaccct cgctcgcccg tcctcgtgcc gatccatcgg gcgacgagca gcaacacgat
     6841 cagtcccacg ccgagcgcga cggctccctc ggccacgggc ccgacccggc cgatcgcggc
     6901 gccgccctcg tcctgcggcc gttgcaggta caacacgtat cgaacgatcg agttcatctc
     6961 ccgatcgctc agatcgaacc ggggcatggc tccttcgccg gctccacacg tacggctcgt
     7021 gttggggcac ccgggtccga ccagcatcgc ctcggcaacc gccaccgccg aggtatggag
     7081 cagaccgggc accacctgtc ccgaggtcag cgcaccgccg gctcccgtgg ccgagtggca
     7141 cgcagcgcag ttctggaggt agagctcggc gccctgcgtg agatcgccgg ccgatggatc
     7201 cggcatggga acgccgggac ccgttccccc gaacgtctcc acgtaggcga cgatcgcgtc
     7261 ggtctgggcg gcgtcgtacc tggacgggcc cgcgaccgct cgctcgtcgg gatcctccag
     7321 cggcatccgc ccggtgacca gcatgaagtg ggtgtaggcg cctccgtcga gcgggccgct
     7381 caggtccggc ccgaacgccg tcccctgtcc ggccgagccg tggcaccagg cgcagtcccg
     7441 ctcgtacagc gtcctcccgg cacccagcgt ggcgacctcc gaggtgatcc cgggcggccg
     7501 gtacggactc gcctcctcct cggtgaagta ggagcaggcc gctcccgcga gggcgagcgc
     7561 cgcggcgacg gggacggcga ctgcgatcca tcggcgcact acgtcaccat cccggctcgg
     7621 acgcgctctg ggcgcgcctg gatgctcggg gtcttgaagg cggccaccag gatccccgcg
     7681 gccacggcga ggtagatcac ggacggtggg atccacatca acgctccggc cagctgttga
     7741 tcctccagag gcgtgagccc ccacagccac ggtccccgtc cgtgtaccgg gtacagcggc
     7801 gcccgggcga agaggagtag cgccccgagc accgcgccct gcagcgccgt cgccacgagg
     7861 aacagcgccc ggccgaggac gccgagcggg gtgaccgatc gcctggacaa cgccacggac
     7921 caggcgagca tcgatgtcag caggaagctc acgtgttcga tcgcgtggac cgcaccgttc
     7981 tcgagcgcga ccgtatacag cagtggagtg tgccacgccc ataggcagat cgtgaacacg
     8041 gtccaggcca cgacgggcgt gcggagcgct ctcgataggc cggacgccgc gaccgcgcgg
     8101 cgaaggcgcg acgccgttcg tcgagccccg gccggaaggc cgaccatcac ggtcgtgacc
     8161 ggtcgacccg cgacgaggag gggagcggcg acgaccacca ggatcaggtg ctgtgtcatg
     8221 tgcgcggaaa acagcgcctc ggcggccgca tccaatggcg tgagcagggc cacggcgacc
     8281 gcgaggatgc cgcaggcgaa gcaggcggcg gcggcggccg agcgacggcg ccaccggcgg
     8341 tatccgcgcg cgtaggcaac gatcgccgag accagcgcga acacgacgag cggatcgaag
     8401 ggccatgcgc cccagaggtc ggccggcgcg acggtggcgt gggcgagcat caccccgcct
     8461 cctggcaccc ggcgatcagc cctgccggca cgaagctcac cgcgatgagg accgtgaaca
     8521 ggatgctcgt catgacccct gcggtagcga gccacgtcgc gcgatcgccg ggcgtgggat
     8581 cgctctgccc ccggaaccgg cgcagctcga tgtaggagcc cacacccgag agcgccgtca
     8641 gtccgaggag cacggcgttc acgatcgcga ggaacgcgtt cacgccgatc cccagtacgt
     8701 cgccggtggc ctgcgtagcc ggcgcgcagg cgaccgcctc ggccaaggcc cagctcgcga
     8761 gcagctggat catccacgcg atcggcccgg tgagcacgcc gagccagctg agcagggacg
     8821 gcgggacccg agatccggcg ggtgcgtcgg gggcggatcg cgcgttcgcc atcacaggtg
     8881 cggggagaca tagatcgtca gcagcacgaa gaaccagacg acgtcgacga agtgccagta
     8941 catcgcgaag ttctgaacgg tgacgtgccg ccgctcgtcg aacgctcccc accacgcccg
     9001 cacttgcacc cacgcggaga acgacaggcc gaccgccacg tgcaggccgt gcaggccggt
     9061 cagcgtgaag aacagagagc cgtacgcgtt ggtacgcggc gtgaactccc gaaggacgcc
     9121 cggatactcg accgccagga gcagcgcgag gaacgcggct cccaggatcc agccgatcag
     9181 caggccgatc cggagcgttc gctggtctcc acggcggatc gcgcgatcgg ccatgtgcac
     9241 ggggacgctc gaggaccaga ggaaggcgct cataatcagc gggagcgcca gctcgggctt
     9301 ggcgatcccg tcgggtggcc agaccggtgc ggagcggaag cgcaggtacc agtaggacac
     9361 gatgatcgtg gcgaacaggg cggcctccgt cgcgatcagc cagaccatcc cccaccaggc
     9421 gaacgaggcg gagccgttcg cggtcgacgg gagcacctcg tccaggcgtt cggtgggctc
     9481 gagctcgtgg gtggcgacgg tcatgtctcc tgggtctccc cgcgcggcca cagccagccc
     9541 atcagcgctc cgaccgagga ggcggccccg gagacggcca cgccccacga ggacaggagc
     9601 aggccgtaga aggtcagcat cagagccatc gtgaggaaca ggggccacgg cgacgcgtgc
     9661 ggcatgcgga cgatggcctg cggctccgcg tcggcgaccg aggtcgacag ggtcgtgtgg
     9721 ccggtggtga gcggccggcc gccccgttcg ggcggttgcg ccccgccgtg gagctcgggc
     9781 tgatcccagg ccggttcgcg gctgcggacc gtggggatcg tctcgaagtt gtactcggga
     9841 ggtggggagc tggtggtcca ctccagagtc tcgcctcccc acgggtcggc cccggcgggc
     9901 tccccgcgtc gctcgctccg gagccagttc caaaacgtca ccacgatgcc ggcgcccatc
     9961 acgaagacgc cgatcgtgga gagcaggttc ggcaggtccc agccgagtcc gccgtgatag
    10021 gtgtacgtgc gtcggggctg cccgagtagc ccggcgatgt gcatcgggaa gaacgtcagg
    10081 ttgaacccga cgaacatcag ccagaagctg atcttgccga gccgctcgtc gagcttgcga
    10141 ccgaggatct tcgggcccca gtggtacagg cccgcgaaca tcgggaacac ggctccgccc
    10201 gcgagcacgt agtggaagtg cgccaccacg aagtacgagt ctgtcgtcgc ctgatcgaac
    10261 gggaccgcgc tgaacatcgc gccggacagt ccgccgatca cgaacagcag gatgaatccc
    10321 agcacgaaca gcatcggcgt cgaaaggacc agccgtcccg agaccatcgt ggcgagccac
    10381 gcgaagatct gcgtgccgct cgggatcacc accatgaagc tggccgccga gatgaagccc
    10441 atcgcgatcg tcggaaggcc gaccgcgaac atgtgatgga cccagacgcc gaagctgatc
    10501 acgaagatga acagctctgc caggacgatg tacgtgtagc cgatcatcgt ccgacgcgag
    10561 aaggtcggga tgatggcgga ggccaggccg agcgccggca acacgacgag atagacctcc
    10621 gggtggccga agaaccagaa caggtgctgc cacaggacgg ggtcgccgcc gccggtcgtg
    10681 tcgtagaagt ggaagccgaa cctgcgctcg agctccagga acacggccgc gatcgtgagc
    10741 gcgggaaggg cgaacaccac ggtgagggcg aaggcgagct cgccccacac gaagatcggg
    10801 atccgattca gcgtcatgcc cggggcgcgc agcttcaaga tcgtcacgat gaagttgatc
    10861 gcccccgcag tggtcgacac gccgatgaag atcaggccga gcgcatagaa gtcgatggcc
    10921 aggcccggcg tgaagttggc gttcgccagc gggacgtagt tgaaccatcc gtcgtccggc
    10981 gcctgtccga cgatgaaggc ggagttcagg aagatgccgg cgcccaggaa cacccagtag
    11041 ccgaacgcgt tgagcctcgg gaacgccatg tcgcgggcgc cgatcatgag agggacgaag
    11101 tagttgccga acccggacag caccggcgtg gcgaacagga acatcatggt cacgccgtgc
    11161 atcgagaaga gctcgttgta cgcctgtggc gacagcaccg agagccccgg tctggcgagc
    11221 tgcacgcgta tgagcaccgc ctcgagcccg gcgacgacga agaacgcgaa ccctgtgtac
    11281 aggtagcgca ggccgatccg cttgtggtcc acggtcccta cccaggacgc gaccccgctc
    11341 ccttcacgcc acaggggctc cagacgctcc ccgtaggagg ccatcaggtc aggccctcga
    11401 ggtactcgac gacggcggac agctcctgcg gtgacaactc ggtcgggggc atgaccgcgc
    11461 cgggcttgac cgactggggg tcggagatcc acgcggcgag gtcgtcgcgc gtgttcgcga
    11521 tcgttccggc cgcgagggta tgcctcgcgg ctacgtgggt gaggtcgggc ccgagcgtcc
    11581 cggaggcctc cgtcccccgg atcgtgtggc agcccacgca ggaggattcg aggaagatac
    11641 ggagccccgc ggttgccctc gggctcggga tgccggcggg tagggactcg ttccggagcc
    11701 accggtcgaa ttcggcgcga ggctcggcca cgacgaagaa cgccatgttc gcgtgttgca
    11761 gcccgcagaa ctcggcgcac tggcccctgt aggtccccga gcgatcggcc cggacgtcca
    11821 ggacgttcgt gcgaccgggg atcatgtcga tcttcggggc gagctccggc acccagaaac
    11881 tgtggatcac gtcgacgctt gagacctcga tgcggaccgt ctccccgacg gggatgtgga
    11941 tctcgttcgc ggtctcgacc ccgtccgggt accgaacctc ccaccaccac atatggccga
    12001 cgacctggat cgtcagctgc gcatcgcggg tatccgtcat cgcgaccagg tcccgcgccg
    12061 agaccaccag gaccgaggtg aggatgacgg cggggacgac cacgccggcg atcacgacga
    12121 acgggtcacc ccatcggggc tcggacgcgt cgacctcgag ccctcgccgg cggcgccgga
    12181 cgatcgcggc cgccatgagc ccggcgacca cgacgaacac cacgagggag atccagaaca
    12241 tcagccacca caggccgttg atgcggcggg cgctgtcgcc ctgaggcgag agcacggagg
    12301 gggcgttgct ggagcatgac gacgccaggg cgatgcaagc gatgatggcc gtcgcccgca
    12361 cccgcccagg ccgcgccccg gacgcgatcg ctccgagcag cctgcgtgcc aagtcggtaa
    12421 agactgttac ccacttcggc ccaactcacg cggcatcagg cctccagctg tcggacgagc
    12481 tggactggcc gggggtgttc tcgcatggat cgcgagcgtg acgctcgggg cgagctggca
    12541 gaggcggcgc aggtcgaccg ggcagacggg gcaggcgacc tgcaccagac gcgcgtgtcc
    12601 tggccagaat cgtcgcgcgg aagtactggc ggacgaagtt cggcatgtgg ctgctggcga
    12661 tagcgttcgg tgcgggtgcc ctggcggcgc tgctaagcgc tcggttgtga gcggctcagg
    12721 atgctacccg gccttggcga atcgcgacca gccgccgtcg ggcgaccacc ggactccggg
    12781 tgctgctgaa gttccacgtg ctgaccatcc aaggtgtgtg acctcgtgcg cgagatgagc
    12841 gcccccagca gatactgcgg gaagtcggta tccccaactg cgcaggcttc cacttcggtt
    12901 tccggacgag atgcgcgcta tgaaggttgg accccggcaa cgtgccgaac gaacgacagg
    12961 atggcgtcgc gcaccctccc ctcgtagctg ctgaagtcga tcgagtcgaa gccaccggaa
    13021 cccgagggga tgaccaccag gtccttgtcc ggcgacgcag tgaggcggta caggcggcga
    13081 acctctccga gcgagacgta ccggtcggac cggaccgtga tgtagagggc cggcacccga
    13141 aggcggggag cggcttggaa ggctccgatc ggcccgaacg ggccctcgac ccattgcccc
    13201 cgaaccgcgg ccggggatat gccgatcacg ccgtcgacgg gcggatcgat ctcggtcgcg
    13261 gcgaccaccg cggctgccac gccggcgtag gacccgagca ggaccacccg agcgaccccg
    13321 cgggacctga gctcgtcgac cgcgccggcc acgtcctgat cgaggcgacc gggagcgtcg
    13381 ctcgcgtccg agttgcctcg gtcccggtag tcgaacacca gcacacggtt cccgcggccc
    13441 ccaaggaacc gggccacggt gagcgtctcg cacagcggat ggggaacgtc gttcgctacc
    13501 acgaccccga cccggcccga cccgatgatc gcgccgtaca ggcgcgttcc gtcggctgcc
    13561 tggaaccaga tcgcacgcgc gttcgcggct ccttcgcaat cgagtgcggg tgcggtgacc
    13621 ggagccggag gtgacgattg ggtcgcgtcc ggcgaaccag acgtggctcc ggtcgataca
    13681 ccggaggatc caggggtgga cgcggaactg caggcggcaa ggacgagggc cgcgaccgag
    13741 gcgatgaccc accatcgaac cgatccggtg gggtcgttcg gcacgggttc ggccccgatc
    13801 gtcccgccgg ccatggaccg cgtcgaggag ttcacggcga gcccctagct ccgagtctcg
    13861 atccagcgca agatcagcgc acgggttcgg tcgccgtggg gtggcacgtc gaccagcgtc
    13921 gtgccgtgag ccgatcccgg atacaggacg atctgcgtgt ccgaggaacc gacacgcttg
    13981 tagacggaca aggcttccct ttgcggggcc cgcgggtcgt ccctcgtccc gacgtagagg
    14041 aacggcgccg acaagctcct caggccagcg gggtcgttga tgccgtagcc ggacaagagc
    14101 cgggtccccg acaggctgat cagcccgtcc acgcggatcc cggcgctgtt ttggaccacg
    14161 gcggcgccgc ccatcgacgc ccccatcagg aacacgcgct tcgctccgtc tgctcgaacc
    14221 tgggccacgg caccggccag atcgtgaccg agcgcaaggc gtgccgtgtc ggggctatat
    14281 gagttcccgt atccacggaa atcgaaggcg aacgcgcgga tccccgcgcg gttgagcgtc
    14341 ttgatgtagg gaacccactc gcacaggtcc gctcctccct cgtgcgcgag caccaccgcc
    14401 gtcttgccgg cccccgactc gatcccgtag aggcgaaccc cgtccgaggc gctgaaccac
    14461 atcgagcgcg cctggacccc gttcgtcgag aagcatgctt cttcgaggct cggcggaggt
    14521 tccggcgcga cggagaccgg gctctccgat gcactccgaa cggttggctt cggtggctca
    14581 ccggtccccg aggacgagca cgccgccatg agaaacgtcg ccacggccgc agcgtgcacc
    14641 agcccgcgcc cggtcaccat gatcccgcct cgccgagcgc gcccccaatc ggccgactct
    14701 ttcgcctgct ccaggggcat cgcgggaact cctttggctc tcttctactc tggacatatc
    14761 tgacttgaca tgtcaaggca gatacgtagc tttatggtca tgtcgttgcg ccacgcgctg
    14821 ctggggttgc tcgcgggttc cccgatgagc ggctacgagc tgaaccgcaa gttcgagcga
    14881 tcgctgttga acgtgtggcc ggcgggccat agccagatct atcccgagct cgctcggctc
    14941 tccgaggccg gcttgatccg gcagagtgga tccggggcgc gcgggaagaa gatctacgag
    15001 atcactcccg aggggctcaa ggaagtgcgg gcatggctgc ggaccgagcc cgaccatgcg
    15061 tcgcgcaacc cggcattcct gcgcgtgttc ttcctgtggc tgatggagcc ggagcaagcg
    15121 atcgcgttcc tcgacaacga ggcccttgtc cacgaggcca agctgatcga gttccaaggg
    15181 atcgagagcc gacggacgcg gggggacgac gaggcgtggg cgttcggtct ggcgctcgag
    15241 tggggaatcc ggtacgaaca ggcgatcctc gagtggaaca gatgggctcg tcaacagatc
    15301 gccgcccggg cggctccgac ttcgggcgtc tcccctagcc cgtcaccttc aacgggacct
    15361 cgctcgtgag gagggtgtat ccccggatga tggactcacg cagccgaatg gactccacca
    15421 gctcaccatc agtagaagca accaacccat caggccaatc agatcgggag ctgctggcaa
    15481 gtgaggccgg agtcagggcg gccgatgtcg tgtggtcggg ccccggccct cgaagcggct
    15541 cgatcccttc ttgaacggac aatccgggta gcgatcaagg tggaggtgcg gctgaccccg
    15601 aaggaggcgt gatgatccag atcgagagtc ctgccctgtc cgaaggcggc gtgattccga
    15661 cccgattcac atgcgacggt gagaacgtct cgccgccgct gcgatggtcg ggcgtccctg
    15721 aggatgcgac cgagctgcgc attagcctga cggaccggga tgctccgcgc ggaacgttca
    15781 cgcactggct cgtcaccggc gtcgaccgcg cgacctcgaa ggtcgaagag ggcgccgccc
    15841 cggtcggcgg taccgaaggg cggaacgact tcggcgacac cgggtacggc gggccgtgtc
    15901 cgcccgccgg ggctccgcac cgctatgtct tcacggtcga ggccctcgac gcggcgggga
    15961 aggttgtcgg atcgggacaa ttgacggcga cctacggacg ctgaggaccg tggaagcaac
    16021 cgagcccgag ggcgaccaga ccaccggatc gccgtgcgac gctggttcgg cgcgtctgcc
    16081 caacgaagcg tccttgcgaa cggcgagaac attcttaaga tacccagaag ctgattcact
    16141 gctccggccg ccagtcttcg gagatcgcgt ccccttatcg tctgccagaa gttagccaca
    16201 gaggagaacg tgcaatgggg aaaccacgca agccaaattc ggcccgacat ccgcctgtag
    16261 catcggatag ccacgccgaa atcgcggatt gggcccggcg cgtgatgcca gacctgcatc
    16321 ccatcgtcaa gcggctggac gaattgatcc gcgagacaat ccccggcctt caatacgccg
    16381 tcaagtggaa gagggcgtac tacgggttgc cagaactcgg gtggatcatc gagatggtcg
    16441 cctacgacgt ctccgtgaat gtcgtcttct tcggcggcgc ggaattcgac cctccgccgc
    16501 cgcttggaac ccccgaacgc acccgctacg tcaagctgaa gaccctggtg gaggcgcagg
    16561 aaccggagat gcacaagtgg atcgagcaag cggggcgcgt gccgggttgg aaattgaatg
    16621 tggacgcgga tgcttgagca gcactatgcc gggcgcctcg atcacgcgga ccgagaactc
    16681 gctcgcgctc tcgagctccg gcacgattgc ggcacgcaga ggagtttggt acgcgcgctc
    16741 cagatgctc
//
LOCUS       DATBRK010000342        13870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1652285, whole genome shotgun sequence.
ACCESSION   DATBRK010000342 DATBRK010000000
VERSION     DATBRK010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13870
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652285"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VIH70_10195"
     CDS             <1..898
                     /locus_tag="VIH70_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEY8116771.1"
                     /translation="FYTPFADALERAEEKFERYVEELDEDCPRCIEEGRTPPGRLQKR
                     LGRYGFFIGCTRFAEEDGGCKYIRNLDGQERAEPEMLDETCPDCGRPLQRKFGRFGPF
                     VGCSGYPDCTYIKKDPPKTTGVTCPQCKQGEIVERRTRFGLMYGCGRYPDCDFAVNHP
                     PVPDHPCPECGSLLIRRPKSLRCWGCGAELDDDFDVTKHGDAEAEGEARAAKSAARAA
                     RAAAKAGAKNAKKKTTAKRTAAKRSSAKRTAAKRTTTKRTTTKRTTTKRKTTEPAAVA
                     SATTEEETDTAADRARVAAAEE"
     gene            899..2908
                     /gene="tmk"
                     /locus_tag="VIH70_10200"
     CDS             899..2908
                     /gene="tmk"
                     /locus_tag="VIH70_10200"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY8116772.1"
                     /translation="MSSFADEVAALRGTRPATFKDLITHPAFSKLLAAQTVSSFGDWV
                     GFVAVTSLVVSKGGEAEAGAYAVAGVMVARTLPAILFGPIAGAIVDRVNRKTIMITAD
                     ISRGALYALMPFLGPLWAIFGVSFVIECFSLLWTPARDASLPNLVPRRQLANANSLGL
                     ITTYATLPIGGLVFAFLSGGSGLLPYFQDNPAFLALWLDAFTFGLSALLVSRIQIPRS
                     APTSFARLDLSRVGKDIMDGIRFLRENSLASSMTLGIVSAFSAVGAVLALGTVFARTT
                     LGAGDPGWGILVTSFGIGMGIGLASSNKVVEFVLREYVFVWSIIAAAGTLLVLAAMPA
                     IGQAAVIAVVLGGFCGSAWVSGYVLLQENVTDEFRGRTFGALTVLSRFGLFLSLTIFP
                     IIAGAIGVHTLTLGERTFDLSGTRISLMIAGVGALLAGLLTRSRLRRFRVGKRERLGL
                     VPKLKRPPTNGLFVAFEGVEGSGKGTQIRLAKEWLESQGIDVMVTREPGGTPIGEELR
                     GLLLAHDTGHIEPKTEALLFAASRAQHVASVIRPALAAGQVVLTDRYIDSSLAYQGSA
                     RGLGEQDVLTLNVWATQGLFPDLVLLLHLEPEAGLLRSLEEPDRIEVEGQDFHAKVSD
                     AYLRMAEEHPERFVVVDADRTPEQVHEQVVEALGRILKEREDHRD"
     gene            2901..4028
                     /locus_tag="VIH70_10205"
     CDS             2901..4028
                     /locus_tag="VIH70_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8116773.1"
                     /translation="MTEARDGAFPVLDRVPGQEHAIAFLRRAAARPHHAYVLAGPEGG
                     GKQLAARAFAAALLCKEGGCGTCRDCRLALEDRHPNEVVVEPEGRDIHVETVRAEIWH
                     PAYRTAPEPGRKVFVIREADRLNPAAADVLLKVLEEPPADAVLMLLSARPDELPETVL
                     SRCHVVSFRPLSEAFVAQTLAAEGIDPGRAGLAARLAGGNLGRARRLATGADGMSFRE
                     AARDALESASRGPAGALEAADLVLVAAERYRKDLGAQLEEELAPFLDERGRAEEAYRG
                     AIRRLEERHKRQVRRAERDYVDRVLLGVSALLRDRVAVAVGGGADVLMNPDLAPAPGD
                     VTSATGAMAAVEEARAALAEDLNLNPRLVLEQAFLRVGRAA"
     gene            4128..4508
                     /locus_tag="VIH70_10210"
     CDS             4128..4508
                     /locus_tag="VIH70_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8116774.1"
                     /translation="MTVRVLIVDDQEPFRLAARMVVDATDGFEVVGEAETGEASVEMA
                     RELGPDLVLMDVNLPGINGLDATRQILADRSDPVIVLLLSTYEEEEYAPRAAECGASA
                     YIPKAVFGPDKLESAWAAANEPAA"
     gene            complement(4505..6598)
                     /locus_tag="VIH70_10215"
     CDS             complement(4505..6598)
                     /locus_tag="VIH70_10215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8116775.1"
                     /translation="MSRGARLAWLLCGCTIVLFAPALFLGIRNGSLSEDPAFAVLAIA
                     MMVGNVSVGAFVASRVPSNPIGWLLMATGIGFVVEIASSEYAVYGLYTDPGALPFTSF
                     AAWFNQWTFLVAIGAILLLIALFPNGSAASRRWRWLPPVIVVDIALAIVTTVLTVGPI
                     DLTDVHPDPPNPTGVEALGSIVGPLQVIAGIGSLALAILAVVSLALRYRASQGEERQQ
                     IRWLAYVGSFSIGLFLLTIASGIGLQQGESTPLNDALFIAFFISIGIGIPAAAAIAVM
                     RYRLWDLDVILKKTIVATILVILLTIASLLVLIVAGGIVVGPLSESPGAALAAGIAVG
                     VLTWPLLRLSRMIADRLVYGTRATPYEVLTQFSGRMAESYATDDILPRMASILGAGTG
                     AKTVTIWLIVGNEVRPATVWPPATEIGSERPRAEATGAVSLEELTGDVFVVRHQGQQL
                     GAITATMHANDPMNPAKEKLIRDLAGQAGLVLRNVRLIEELRASRRRLVAAQDAERRR
                     LERNIHDGAQQQLVALQVKQRLVQGMIERDPAKALELMTQLQVDTTGALDDLRDLARG
                     IYPPLLADQGLGPALESQARKSPVPISVETDGVERYPQDVEAAVYFCALEALNNLAKY
                     AKASAATMSLSQSDGMLTVAVSDDGVGFVVGERSSSGTGLQGMADRLDAIGGSLEIRS
                     APGEGTTVLGRVPVG"
     gene            complement(6595..8817)
                     /locus_tag="VIH70_10220"
     CDS             complement(6595..8817)
                     /locus_tag="VIH70_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8116776.1"
                     /translation="MTQRTAKIVSASILVAVIAAFGALISLDVIGGDRLSHPPIVVVG
                     DPDTPGMAPVRADLEETLRRGGELRSSQALTDVVIAILVAGWGITGWLIVSRQPRNTA
                     GWLFCVGAATVAVAGLSTAYTIYGIRLASSRLPGQDQVALVGDYSIIAVALIPLLVLL
                     FPDGHPPSARWRWAIWTLLAGIGVAATAYALTPGPLNNFVEYGILYANPYGFGAFAEG
                     RPGNALVAIGTLMIVGASLATVVAVWQRFRRARGEERQQMRWLVAVATVAGVGIGGIL
                     LLFPLVPILGLDEGGIGDVIFPILSIPALLAIFVGIPAAYLIAIFKHGLWDLDVVIKK
                     ALVAFVLTLLLVGAGLGAIFTFGQPALWGGNRVGVAIGVVAGLLIWPLIRLARRAARR
                     VVFGRRVAPYEVMSAFAERVGETYSTEDVLPRMAQILAEGTAATGARVWLQVGGELRA
                     EARWPIGLEDGEPLPVVGDELPRIEGESAFEVRDRGQLLGALSVVMPASDPMNPSKER
                     LVRDLASQAGLVLRNVRLIQELRASRQRLVAAQDEERRKLERDIHDGAQQQLVALGVK
                     MRLLGTFVDGDLERARALVEQLQTETIDALENLRDLARGIYPPLLADQGLPAALEAQG
                     RKVAIPVSVQPDGVGRYRQEVEATVYFCVLEALQNVSKYADASHIIIGLREDDGRLVF
                     EVHDDGVGFDPARSSYGTGLQGMADRVEALGGRLSIESAPGRGTTLIGRVPIPGGAPR
                     "
     gene            complement(8891..9046)
                     /locus_tag="VIH70_10225"
     CDS             complement(8891..9046)
                     /locus_tag="VIH70_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116777.1"
                     /translation="MWLTTLLIWIVDKMLAFATADEGPGFEVSTTGAGEGMQIMRDRI
                     AGLAASS"
     gene            9252..9659
                     /locus_tag="VIH70_10230"
     CDS             9252..9659
                     /locus_tag="VIH70_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8116778.1"
                     /translation="MTKILVGTDTSASADLAVGEAARLAGQTGAELLVLHVRPGSAVR
                     DAVDPKKSADPVGYLAGLERRFPSITVRGWAEPGDPAERLVAVAEQERVETIVLGNRG
                     THGSWWRVRDSVPNLVLRHAPCSVMIVDTRAAR"
     gene            complement(9676..10347)
                     /locus_tag="VIH70_10235"
     CDS             complement(9676..10347)
                     /locus_tag="VIH70_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8116779.1"
                     /translation="MVRVVFAEDNYLVREGTAALLQTSEEVELIGTASSFDELMTAVE
                     ELKPEAVLTDIRMPPSNTTEGIDAARRIRADHPDIGVVVLSQFAEEAYAYELLKDGAA
                     GLGYLLKERVANLEELVRALNEVTKGGSVLDPKVVEALVSAKDRMAHSPLGQLTDRER
                     EVLENMAQGKNNASIAKSLFLTERAVEKHINSLFHKLGLSEETDVHRRVKAVLAFLRE
                     SDHPA"
     gene            10383..11678
                     /locus_tag="VIH70_10240"
     CDS             10383..11678
                     /locus_tag="VIH70_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8116780.1"
                     /translation="MSIRVVLGEDNVLVREGVRALLDSYDDVEVVGVAEDAPSLLAAA
                     AEHVPEVVVTDIKMPPNFQLEGIDCAHAIREKHPGTGVVVLSAHDDEAYAIALLGKGQ
                     SGLAYLLKDRIAQGDELVRAIREVAAGGSAVDPAIAERLSGRRETAEHDRELLELMSQ
                     GLGYEQMAKLLGTTQEAVDRRVTDLFTKMAAGAGSGGASAVDDMKRLHAAVVAKQAAA
                     ATLSSYVPQRVAEKLARGEVGREELEVTVLFSDIRGFSTMAERLAANQIAEIVGRHLS
                     AMAEIVVDHLGMIDKFQGDAVMAVFGAPDPIDDHAERALRCAIAMQRRQAELNAEGWG
                     TDAVDGLHIGVGVNTGQVIAGAIGGGGRLEYTVIGDAVNVAERLQSEAEGGEIIASST
                     TIASAPGVSSEPIGSRQVKGREEPVEVFRVLVTEPEVAP"
     gene            11675..13798
                     /locus_tag="VIH70_10245"
     CDS             11675..13798
                     /locus_tag="VIH70_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8116781.1"
                     /translation="MTQRTAARLAWGILAFIAVSAVVSITLSIAGQRYDVFFVAVLAI
                     FPVVGVVLASRRPSNPLGWIMLGIGVVWALPSASYGVYGLTEGRHLPGAADALALDGP
                     TWVWFIGGSGFLLLLFPDGHLPTRSWRWFAWTMGAGMAVLTLFFLVLPGNFTDYDLPH
                     VRNPMGIEALRPLKPFILALVLTVPLTVVGGAVAVIGRLRRASDDVERHQLRWLAWAG
                     GVVAVSYVLAFLSTGWTNETAIWGAVLQFLAFWSFGLIPVAIGVAVLRYRLYDIDVVI
                     RKTVVVGVLAAFIALVYVAIVAGAGAVVGASGNALASALAAAVVAIAFQPVARRARRL
                     ADRVVYGRRASPYEALSAFSERVGETYATEDLLPRMARILGEATGARRADVWLKIGER
                     FRLAASWPSDAGSLPDRSAPDGELPWLPEADASFPVRHRGELLGALALAKPVSDPLST
                     TDERLASDLAGQAGLVLSNARLTTELESRIDELHELHKRLVSAQDRERRRLERNIHDG
                     AQQQLVALSVKLRLAQSLVAKDPDQTGRILEQLQTETTETLEDLRDLARGIYPPLLAD
                     KGLVAALESQTRRSVVPVEVHPDGTGRYPQEVEAAVYFSVLEALQNVAKYAHASRAGV
                     RLLASDSVLVFEVDDDGVGFDPASTGYGTGLQGIADRLAALDGRIEIRSGPGGGTVVT
                     GTIPLGSDPARDGLPTPSVERDETR"
BASE COUNT         2071 a         4767 c         4957 g         2075 t
ORIGIN      
        1 gttctacacg ccgttcgccg acgccctcga gcgcgcggag gagaagttcg agcgttacgt
       61 ggaggagctc gacgaggact gcccacgttg catcgaggag ggacgcaccc cgcccggccg
      121 gttgcagaag cgcctcgggc ggtacggctt cttcatcggg tgcacccggt tcgccgagga
      181 ggacggcggc tgcaagtaca tccgcaacct cgatggccag gaacgggccg agccggagat
      241 gctcgacgag acctgtccgg actgcggccg cccgctgcag cgcaagttcg gtcgcttcgg
      301 accgttcgtg ggctgctccg ggtacccgga ctgcacgtac atcaagaagg acccaccgaa
      361 gaccaccggc gtcacctgtc ctcagtgcaa gcagggcgag atcgtcgaga ggcggacgag
      421 gttcgggctg atgtacgggt gcgggcggta ccccgactgc gacttcgcgg tcaaccatcc
      481 tccggtgccc gaccatccgt gccccgagtg tggttcgctc ctgatccgaa ggccgaagag
      541 cctgcgctgc tgggggtgcg gcgccgagct cgacgacgat ttcgacgtca cgaagcacgg
      601 tgatgccgag gccgagggcg aggccagggc cgcgaagtcg gcagcccggg ccgcgcgcgc
      661 ggcggccaag gccggcgcga agaacgcgaa gaagaagacg acggccaagc ggacggccgc
      721 gaagcggagc tcggccaagc ggaccgccgc caagcgcacg acgaccaagc gcacgacgac
      781 caagcgcacg acgacgaagc ggaagacgac cgagccggcc gcggtcgcga gcgcgaccac
      841 cgaggaggag acggacaccg cggcggatcg ggcccgcgtg gcggccgcgg aggagtaggt
      901 gtcgtccttc gccgacgagg tcgccgcact ccgcgggacc cggccggcga ccttcaagga
      961 cctcatcacc catcccgcct tctccaagct gctggccgcg cagaccgtca gctcgttcgg
     1021 ggactgggtc gggttcgtcg cggtgacttc tctggtggtc agcaagggcg gcgaggctga
     1081 ggccggtgcc tacgcggtcg ccggcgtgat ggtcgcccgg acgctccccg cgatcctgtt
     1141 cggccccatc gccggcgcga tcgtcgacag ggtgaatcgc aagaccatca tgatcacggc
     1201 ggacatctcg cggggggcgt tgtacgcgct catgccgttt ctcggcccgc tctgggcgat
     1261 cttcggcgtc tccttcgtga tcgaatgctt ctcgttgctg tggacgccgg cccgcgacgc
     1321 ctcgctgccg aacctcgtcc cgcgacggca actcgcgaac gcaaactcgc tcggcctgat
     1381 cacgacgtac gcgacgctcc cgatcggcgg cctggtgttc gcgtttctgt cgggaggctc
     1441 ggggctgtta ccgtatttcc aggacaaccc ggcgttcctc gcgctgtggc tcgacgcgtt
     1501 cacgttcgga ctctcggcct tgctcgtcag caggatccag atcccgcggt cggcgccgac
     1561 ctcgttcgcg aggctcgacc tgtcgcgcgt cggcaaggac atcatggacg gcatccggtt
     1621 cctgcgcgag aactccctcg cgtcctcgat gacgctcggc atcgtctcgg cgttcagcgc
     1681 cgtgggagcc gtgctcgccc tcggtaccgt gttcgcgagg acgacgctcg gcgcgggcga
     1741 cccggggtgg gggatcctcg tgacctcgtt cggtatcggg atggggatcg ggctggcgtc
     1801 ctcgaacaag gtcgtcgagt tcgtccttcg cgagtacgtc ttcgtgtggt cgatcatcgc
     1861 ggccgccgga acgctgctcg tgctcgccgc gatgcccgcc atcgggcagg ccgcggtcat
     1921 cgcggtggtg ctcggcgggt tctgcggctc ggcgtgggtg agcgggtacg tcctcctgca
     1981 ggagaacgtg accgacgagt tccgtggacg gacgttcggt gcgctcacgg tgctctcgcg
     2041 gttcggcctg ttcctgtcgc tcacgatctt cccgatcatc gccggggcca tcggtgtgca
     2101 cacgttgacg ctgggcgagc gcacgttcga cctgtcgggg acgcggatct cgctgatgat
     2161 cgcgggggtg ggagcgctgc tcgcgggcct gctgacccgg agcaggctcc ggcggttccg
     2221 ggtcggcaag cgggaacggc tcgggttggt ccccaagctg aagcgaccgc cgacgaacgg
     2281 cctgttcgtc gccttcgagg gtgtcgaggg ttcgggcaag gggacgcaga tccgccttgc
     2341 gaaggagtgg ttggagtccc agggcatcga cgtcatggtg acgagggagc ccggagggac
     2401 gcccatcggc gaggagcttc ggggcctgct gctcgcgcac gatacaggtc acatcgagcc
     2461 gaagaccgag gccctgctct tcgcggcgag ccgggcgcag cacgtggcct cggtgatccg
     2521 gcccgcactg gccgcgggac aggtcgtgct gacggaccgg tacatcgatt cgtccctggc
     2581 ctatcagggt tcggcgcgcg gactcggcga gcaggacgtc ctgacgctca acgtgtgggc
     2641 gacccagggg ctgttccccg acctcgtgct cctgctccac ttggagccgg aggcgggcct
     2701 gctgcgttcg ctcgaggaac ccgatcgcat cgaggtcgag ggtcaggact tccacgcgaa
     2761 ggtctccgac gcgtacctgc ggatggccga ggagcacccg gagcggttcg tcgtcgtgga
     2821 cgcggaccgg acgccggagc aggtccacga gcaggtggtc gaggcgctcg gcaggatctt
     2881 gaaggagcgg gaggaccatc gtgactgagg cgcgtgacgg cgcgttcccg gtgctcgacc
     2941 gcgtccccgg tcaggagcac gcgatcgcgt tcctgcgacg ggccgcggcg cgaccgcacc
     3001 acgcgtacgt gctggccggc cccgagggcg gaggcaagca gctcgccgcg cgggccttcg
     3061 cggcggcgct gctgtgcaag gagggtgggt gcgggacctg ccgggactgc cggctggcgc
     3121 tcgaggaccg tcacccgaac gaggtcgtcg tcgaacccga gggccgcgac atccacgtgg
     3181 agaccgttcg cgccgagatc tggcaccccg cgtatcggac ggcccccgag ccgggtcgca
     3241 aggtcttcgt gatccgggag gccgatcggc tgaacccggc cgcggccgac gtcctgttga
     3301 aggtgctcga ggagccgccc gcggatgcgg tgctgatgct cctctcggcc cggcccgacg
     3361 agctgcccga gaccgtcctg tcccgctgcc acgtggtctc gttccgcccg ctctcggagg
     3421 cgttcgtcgc gcagacgctc gccgccgagg ggatcgaccc cggtcgggcc ggcctcgccg
     3481 cccggctcgc cggcgggaac ctcgggcgag cccgccggct cgcgacgggc gccgacggga
     3541 tgtcgttccg cgaggcggcc cgcgatgccc tcgagagcgc ctcgcgcgga cccgccggtg
     3601 ccctcgaggc cgccgatctc gtgctggtcg cggccgagcg ctaccgcaag gacctcggcg
     3661 cgcagctgga ggaggagctg gcgccgttcc tggacgaacg gggtcgggcg gaggaggcct
     3721 accgcggagc gatccgccgg ctcgaggagc ggcacaagcg acaggtccgc cgggccgaac
     3781 gcgactacgt ggaccgcgtg ctgctcgggg tctcggcact gctgcgcgat cgcgtcgcgg
     3841 tggcggtcgg gggcggcgcc gacgtgctga tgaacccgga tctcgcgccc gcgccgggcg
     3901 acgtgacctc ggcgaccggc gcgatggccg cggtcgagga ggcccgcgcc gcgctcgccg
     3961 aggacctgaa cctcaacccg cggctcgtgc tcgagcaggc gttcctccgg gtcggccgcg
     4021 ccgcgtagcg cctgccagta cgccagttcg ggcatccgaa cagtggtgtt ggcatcactg
     4081 tcggagcgag gccccggtgg gacgctttac gtcggaggac aacggcgatg acggttcgag
     4141 tgctcatcgt ggacgaccag gagccgttcc ggctggcggc gcgcatggtg gtggacgcca
     4201 ccgacggctt cgaggtggtc ggggaggccg agaccggcga ggcctcggtc gagatggcca
     4261 gggagctcgg acccgacctc gtgctgatgg acgtgaacct accgggcatc aacgggctcg
     4321 atgccacgcg ccagatcctg gccgatcgtt cggacccggt gatcgtcctc ctgctctcca
     4381 cgtacgagga ggaggagtac gcgccccggg ccgccgagtg cggcgcgtcc gcgtacatcc
     4441 ccaaggccgt gttcgggccc gacaagctcg agtccgcgtg ggcggccgcc aacgagccgg
     4501 ccgcctaacc gaccgggacc cgacccagca ccgtcgtccc ctctccgggc gccgaccgga
     4561 tctcgagcga gccgccgatc gcgtcgagcc ggtccgccat cccctgcagg ccggtgccgc
     4621 tcgacgaccg ctctccgacg acgaacccga cgccgtcgtc ggagacggcg accgtcagca
     4681 tcccgtccga ctgcgacagc gacattgtcg ccgccgacgc cttcgcgtac ttcgcgaggt
     4741 tgttcagcgc ctcgagcgcg cagaagtaga cggcggcctc gacgtcctgc gggtaccgtt
     4801 cgacgccgtc ggtctccacc gagatcggga ccggcgactt gcgcgcctgc gactcgagcg
     4861 cgggtccgag cccctggtcc gcgagcagcg gcgggtagat cccccgcgcg aggtcgcgga
     4921 gatcgtcgag ggctcccgtc gtgtccacct ggagctgcgt catcagctcg agcgccttcg
     4981 cggggtcccg ttcgatcatg ccctgaacca ggcgctgctt gacctggagc gcaacgagct
     5041 gttgctgcgc accgtcgtgg atgttgcgtt cgagtcgacg ccgctcggcg tcctgagcgg
     5101 cgacgagcct tcggcgtgac gcccggagtt cctcgatcag ccgcacgttg cgcagcacga
     5161 ggccggcctg tcctgcgagg tcgcggatca gcttctcctt cgccgggttc atcgggtcgt
     5221 tcgcgtgcat cgtcgccgtg atcgcgccga gctgctgccc ctggtgccgg accacgaaca
     5281 catcgccggt gagctcctcg agggagacgg ccccggtcgc ctcggccctc gggcgctccg
     5341 aaccgatctc ggtcgcgggc ggccagacgg tggccggcct gacctcgttg ccgacgatca
     5401 gccagatcgt cacggtcttc gcgcccgtcc cggcgccaag gatcgacgcc atcctcggca
     5461 ggatgtcgtc ggtggcatac gactccgcca tccgacccga gaactgggtc aggacctcgt
     5521 atggcgtggc gcgcgtaccg taaacgaggc ggtccgcgat catgcgcgac agccgcagga
     5581 gcggccaggt cagcacgccc acggcgatcc ccgccgccag ggccgcgccc ggcgactcgg
     5641 acagcggccc gacgacgatc ccgccggcga cgatcaggac caggagcgag gcgatcgtca
     5701 ggaggatcac gaggatcgtt gcgacgatcg tcttcttcag gatgacgtcg agatcccaca
     5761 ggcggtaccg catgacggcg atcgccgcgg cggccgggat cccgatgccg atcgagatga
     5821 agaacgcgat gaacagtgcg tcgttcagcg gcgtggactc accttgctgc aagccgatcc
     5881 cgctcgcgat cgtcaggagg aagagaccga tcgaaaacga gccgacgtac gcgagccacc
     5941 tgatctgctg tcgttcctca ccctgcgaag cgcggtaccg gagcgccagg gacacgacgg
     6001 cgaggatcgc gagggcgagc gacccgatgc cggcgatcac ctgcaacggt ccgacgatgc
     6061 tccccagtgc ttcgacgcct gtggggttgg gcgggtcggg gtgcacatcg gtcaggtcga
     6121 tcggaccgac cgtgaggacg gtggtgacga tcgcgagcgc gatgtcgacg acgatgaccg
     6181 gcgggagcca ccgccaccgg cgcgacgcgg ccgaaccgtt ggggaacagc gcgatcagga
     6241 gcaggatggc tccgatagcg acgaggaacg tccattggtt gaaccacgcc gcgaacgagg
     6301 tgaacgggag cgctccggga tccgtgtaca gcccgtagac cgcgtactcg gacgaggcga
     6361 tctcgaccac gaatccgatc ccggtcgcca tgaggagcca gccgatcggg ttggacggga
     6421 cgcgcgaagc cacgaacgcc ccgaccgaca cgttcccgac catcatggcg atggccagga
     6481 cggcgaaagc gggatcctcc gagagcgagc cgttgcggat gcccaggaag agcgcgggcg
     6541 cgaacaggac gatggtgcag ccgcacagga gccacgcgag cctggcgccc ctgctcatcg
     6601 aggcgcccct cccgggatcg gaacgcgacc gatcagggtg gtcccacggc cgggggcgct
     6661 ttcgatgctc agccggccgc ccagcgcctc cacgcgatcc gccatgccct gcagcccggt
     6721 cccgtagctc gacctagcgg gatcgaagcc aacgccgtcg tcgtggacct cgaacaccaa
     6781 ccgcccgtcg tcctcccgca ggccgataat gatgtggctg gcatccgcgt acttactgac
     6841 gttctgcaac gcctcgagca cacagaagta gacggtcgcc tcgacctcct gcctgtaccg
     6901 gccgaccccg tcgggttgga ccgacacggg gatggcgact ttgcggccct gcgcctccag
     6961 cgcggccggc aagccctggt cggccagcag cggcgggtag ataccccgcg ccaggtctcg
     7021 aaggttctcg agcgcgtcaa tcgtctccgt ctgcaactgc tccaccaacg ctcgggccct
     7081 ctcgagatcg ccgtcgacga acgtcccgag cagccgcatc ttgacgccga gcgccaccag
     7141 ctgctgctgg gcgccgtcgt ggatgtcgcg ctcgagcttg cgtcgctcct cgtcctgcgc
     7201 ggccaccaga cgctgccgcg aggctcggag ctcctggatc agtcggacgt tgcggagcac
     7261 gagacccgcc tgagaggcga gatctcggac cagccgctcc ttcgacgggt tcatcgggtc
     7321 gctcgcgggc atcacgaccg agagcgcccc gagcagctgg ccccggtcgc ggacctcgaa
     7381 ggccgattcg ccctcgatcc gggggagctc gtcaccgacg accgggaggg gctctccatc
     7441 ctcgaggccg atgggccagc gggcctcggc acgcagttcg ccaccgacct ggagccaaac
     7501 ccgcgcgccc gttgcggcgg tcccttcggc gaggatctgc gccatccgcg gcagcacgtc
     7561 ctcagtggag tacgtctccc cgacgcgttc ggcgaacgcg ctcatcacct cgtacggagc
     7621 cacgcgccga ccgaagacca cgcgtcgcgc ggctcttcgc gcgagcctga tgagcggcca
     7681 gatcaggagc ccggcgacga cgccgatcgc cacaccgacg cggttgccgc cccatagcgc
     7741 cggctgcccg aaggtgaaga tcgcgccgag gccggcaccc accagcagga gcgtgagcac
     7801 gaacgcgacg agcgccttct tgatcacgac gtcgaggtcc cacaggccgt gcttgaagat
     7861 cgcgatcagg taggcggcgg ggatcccgac gaagatcgcg agcagcgccg ggatggacag
     7921 gatggggaag atcacgtcgc cgatcccgcc ctcgtcgagc ccgaggatcg ggacgagcgg
     7981 gaagagcagg aggatcccac cgatccctac gccggcgacc gtcgcaacgg cgaccaacca
     8041 ccgcatctgc tggcgttcct cgccgcgggc tcgccggaac cgctgccaga cggcgacgac
     8101 cgtcgcgagg ctcgcgccga cgatcatgag cgtgccgatg gcaacgagcg cgttgccggg
     8161 gcgtccctcc gcgaacgctc cgaacccgta cgggttcgcg tacaggatcc cgtactcgac
     8221 gaagttgttg agcgggccgg gcgtgagcgc gtacgcggtc gccgcgacgc cgatgccggc
     8281 gaggagggtc cagatcgccc atcgccatcg ggcgctcggt gggtgcccgt ccgggaagag
     8341 caggaccagc aacgggatca gcgcgacggc gatgatcgaa tagtctccga cgagcgcgac
     8401 ctgatcctgg cccggcaatc gactcgaggc cagccggatg ccgtagatcg tgtaggcggt
     8461 cgagagcccg gcaaccgcga cggtggcggc gccgacacag aagagccacc cggcagtgtt
     8521 ccggggctgt cgcgacacga tgagccatcc cgtgatgccc cagccggcca ccaagatggc
     8581 gatgaccacg tcggtcaagg cctgcgacga gcggagttcc ccgccgcggc ggagcgtttc
     8641 ctcgaggtcg gcgcggacag gggccatacc cggcgtgtcg gggtcgccga ccacgacgat
     8701 cgggggatgc gagaggcggt cgccgccgat gacgtccaag ctgatcaggg ccccgaatgc
     8761 ggcgattacg gcgaccagga tcgaggccga tacgatcttc gctgtccgct gggtcacacc
     8821 gatgacgcct ccatgacccg ggtgggcacc cgcccggtga tggtcgtgcc tcgccccgga
     8881 aggctctcga tcatgagctt gccgccaggc ccgcgatccg gtcccgcatg atctgcatcc
     8941 cctcgcccgc gccagtcgtc gagacctcga acccgggccc ctcgtccgcg gtcgcaaacg
     9001 ccagcatctt gtcgacgatc cagatcagga gcgtggtgag ccacacgccg tgagccagcc
     9061 ggtcgaggcc ttcatgcggc gaatcgtgac agtcgggcgt gcgggttcca aggggggtca
     9121 ccctaggaag ccagtgcacg aacctccccc ttcgggggtg ctggcatccc ccacatgttg
     9181 tggtggcgac cggctcgaac ccgccggcga ctacgcgtag cgtgagcgtc gcccgaagga
     9241 ggcgaacctc gatgacgaag atcttggtgg gaacggatac ctcggcctcg gccgatctcg
     9301 cggtgggcga ggccgcccgg cttgctggcc agaccggagc cgagttgctg gtcctccacg
     9361 ttcgacccgg gtcagcggtc cgcgacgccg tcgacccgaa gaagtctgcc gacccggtcg
     9421 gctacctcgc cggcctcgaa cggcggttcc cctccatcac cgtccgaggc tgggcggagc
     9481 cgggggaccc ggccgagcgg ctggtggcgg tcgccgaaca ggaacgcgtc gagacgatcg
     9541 tcctggggaa ccgggggacc cacgggtcgt ggtggcgcgt gcgcgacagc gtgccgaacc
     9601 tggtgctccg gcacgcgccg tgctcggtga tgatcgtaga cacccgcgcg gcccggtgag
     9661 atcgggccgg tgaggtcagg cggggtggtc gctctcgcgc aggaacgcga gcaccgcctt
     9721 gacccggcgg tggacgtcgg tctcctcgga gagtccgagc ttgtggaaga gggagttgat
     9781 gtgcttctcg acggcacgct cggtcaggaa cagcgacttg gcgatcgacg cgttgttctt
     9841 gccctgtgcc atgttctcga ggacctcgcg ctcccggtcg gtgagctgcc cgagcgggga
     9901 gtgcgccatc cggtccttgg ccgagacgag cgcctccacc accttgggat cgaggaccga
     9961 gccccctttg gtcacctcgt tgagcgcgcg gaccagctcc tcgaggttgg ccacgcgctc
    10021 cttcagcagg tacccgagcc ccgccgcccc gtccttcagc agctcgtacg cgtaggcctc
    10081 ctccgcgaac tgagagagca cgacgacgcc gatgtcggga tgatcggctc ggatgcgtcg
    10141 ggcggcgtcg atcccctcgg tcgtgttcga ggggggcatg cggatgtcgg tcaggaccgc
    10201 ctcgggcttc agctcttcga ccgccgtcat gagctcgtcg aacgagctcg cggtgccgat
    10261 cagctcgacc tcctcggagg tctggagcag tgccgcggtg ccctcccgca cgaggtagtt
    10321 atcctccgcg aagaccacgc gcaccatgcg cgcatcgtac cgtcgtcgga gaggcaggca
    10381 gcgtgagcat cagggtcgtc ctcggcgagg acaacgtcct cgtccgcgag ggcgttcgcg
    10441 cactcctgga ctcctatgac gacgtcgagg tcgtgggcgt ggccgaggac gccccgtcgc
    10501 tcctcgcggc cgcggccgag cacgttccgg aggtcgtcgt caccgacatc aagatgcccc
    10561 cgaacttcca gctcgagggg atcgactgcg cgcacgcgat ccgcgagaag cacccgggga
    10621 cgggcgtcgt cgtgctctcg gcccacgacg acgaggcgta cgcgatcgca ctcctcggca
    10681 agggccagag cgggctcgcc tacttgttga aggaccggat cgcgcagggc gacgagctcg
    10741 tccgcgcgat ccgcgaggtc gcggccggcg ggagcgcggt cgaccccgcg atcgccgaac
    10801 gcctgtctgg ccggcgcgag accgccgagc acgaccgcga gctcctcgag ctcatgtcgc
    10861 aggggctggg ctacgaacag atggcgaagc tgcttgggac cacgcaggag gctgtcgacc
    10921 ggcgggtcac ggacctgttc acgaagatgg cggccggcgc gggttccggc ggcgcgagcg
    10981 cggtggacga catgaagcga ctgcacgccg ccgtcgtcgc caagcaggcc gcggcggcca
    11041 cattgtcctc ctacgtgccg cagcgcgttg ccgagaagct cgcccggggc gaggtcggtc
    11101 gcgaggagct cgaggtgacg gtgctcttca gcgacatccg gggcttctcg acgatggccg
    11161 aacggctggc ggcgaaccag atcgccgaga tcgtcgggcg tcacctctcg gcgatggccg
    11221 agatcgtggt cgatcacctg gggatgatcg acaagttcca gggcgacgcg gtgatggccg
    11281 tgttcggagc gccggacccg atcgacgacc acgccgaacg cgcgcttcgg tgcgcgatcg
    11341 cgatgcagcg tcgccaggcc gagctcaacg ccgagggatg gggcaccgac gccgtcgacg
    11401 gcctgcacat cggggtgggt gtgaacaccg gtcaggtgat cgcgggtgcg atcggcggcg
    11461 gcggccggct cgagtacacg gtgatcggcg acgccgtgaa cgtcgccgag cggttgcaga
    11521 gcgaggcgga gggcggcgag atcatcgcga gctcgaccac gatcgcctcg gcgccgggcg
    11581 tctcgtccga gccgatcggt tcgcgacagg tgaagggccg agaagagccc gtcgaggtct
    11641 tccgcgtcct ggtgaccgaa cccgaggtcg ctccgtgacg cagcgcacgg ccgctcgact
    11701 cgcgtggggc atcctcgcgt tcatcgcggt ctctgccgtc gtctcgatca cgctctcgat
    11761 cgcgggccag cgctacgacg tcttcttcgt cgccgtcctg gcgatcttcc ccgtcgtcgg
    11821 cgtcgtgctg gcgtcccggc gcccgtcgaa cccactcggc tggatcatgc tgggcatcgg
    11881 cgtcgtgtgg gccctgccgt cggcctcgta cggcgtctac ggcctgacgg aggggcggca
    11941 tctgccggga gccgccgacg cgctcgccct ggacggtccg acgtgggtgt ggttcatcgg
    12001 cggcagcggc ttcctgctgc tgctgttccc cgacggccac ctgccgactc gatcgtggag
    12061 gtggttcgcg tggacgatgg gcgcggggat ggcggtcctc acgctgttct tcctcgtctt
    12121 gcccggcaac ttcaccgact acgacctgcc gcacgtccgg aacccgatgg gcatcgaggc
    12181 gctccgcccc ttgaagcctt tcatcctcgc gctcgtcctc acggtgccgc tcaccgtcgt
    12241 gggaggcgcc gtcgccgtga tcggaaggct gcgccgcgcg agcgacgacg tcgagcggca
    12301 tcagctccgc tggctcgcgt gggcgggcgg cgtggtcgcg gtgagctacg tcctggcgtt
    12361 cctcagcacc ggctggacca atgagaccgc gatctggggg gcggtgctcc agttcctcgc
    12421 gttctggtcg ttcggactga tccccgtcgc gatcggcgtc gcggtgctcc gatacaggct
    12481 gtacgacatc gacgtcgtga tccgcaagac ggtggtcgtt ggcgtgctcg cggccttcat
    12541 cgccctcgtc tacgtcgcca ttgtggcggg cgccggagcg gtcgtgggcg caagcgggaa
    12601 cgccctggcg tctgcgctcg cggcggcggt cgtggcgatc gcgttccagc cggtcgcgcg
    12661 ccgggcccgt cggctcgcgg atcgtgtcgt gtacggacgc cgcgcctcgc cgtacgaggc
    12721 gctgtcggcg ttctccgagc gcgtcgggga gacctacgcc accgaggacc tgctgcccag
    12781 gatggcgcga atcctgggcg aggcgaccgg cgctcgcaga gccgacgtgt ggttgaagat
    12841 cggcgagcgc ttccgcctgg ccgcgtcgtg gccttccgac gccggatcgc ttcccgaccg
    12901 gtcggcgccc gacggggagc tgccctggtt gcccgaggcg gacgcgtcgt tccccgtccg
    12961 ccatcggggc gagctcctcg gggcgctcgc gctcgccaag cccgtctcgg atcccttgtc
    13021 cacgaccgac gaacggctcg cctcggacct cgcaggccag gccgggctcg tcctcagcaa
    13081 cgcgcgactc acgacggagc tcgagagcag gatcgacgaa ctccacgagc tccacaagcg
    13141 gttggtgtcg gcacaggacc gggaacgtcg ccgcctcgaa cgcaacatcc acgacggtgc
    13201 gcaacaacag ctcgtcgccc tctccgtcaa gctccggctc gcccagtccc tcgtcgcgaa
    13261 ggaccccgac cagaccggac ggatcctcga gcagctgcag acggagacca ccgagacgct
    13321 ggaggacctg cgcgatctcg ctcgcgggat ctacccgccg ctgctggcgg acaaggggct
    13381 cgtcgcggca ctggagtcgc agacgcgtcg atccgtggtt cccgtcgagg tccatccgga
    13441 cggcaccggc cggtacccgc aggaggtcga ggccgccgtg tatttctcgg tgctcgaggc
    13501 gctccagaac gtggcgaagt acgcgcacgc atcacgcgcg ggggttcggc tgctcgcgtc
    13561 cgactcggtg ctggtgttcg aggtcgacga tgacggggtc gggttcgatc cggcttcgac
    13621 cggatacggg acggggctcc agggcatcgc cgaccgtctc gccgccctgg acggacgcat
    13681 cgagatacgg tcggggcccg gcggcggaac ggtggtgacc ggcacgatcc cgctcgggag
    13741 cgacccggcg cgagacggcc tcccgacgcc gagcgtggaa cgggacgaga cgagatgaag
    13801 tggccggcgc gctcgaggcc ctcccggtgg ccgtgggtcg ccttgggtgg gttcttctcg
    13861 ctcgcgattg
//
LOCUS       DATBRK010000343         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_498232, whole genome shotgun sequence.
ACCESSION   DATBRK010000343 DATBRK010000000
VERSION     DATBRK010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498232"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /gene="truB"
                     /locus_tag="VIH70_10250"
     CDS             complement(<1..578)
                     /gene="truB"
                     /locus_tag="VIH70_10250"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY8116782.1"
                     /translation="MTPAPEGLLLVDKPSGMTSHDAVEVVRRSLGTRKVGHAGTLDPM
                     ATGLLVLGVGRATRLLRYLGELPKTYAATGRLGEETDTLDADGEIVRTAPVNVSLAEV
                     ERACASLVGESMQAPPAYSAVRVGGRRLYEAARRGEVLEARPRPIRVDAFAVSAFDGR
                     DLEARVTCSGGTYVRVLVADVGRSLGCGAHLVR"
     gene            complement(585..1565)
                     /locus_tag="VIH70_10255"
     CDS             complement(585..1565)
                     /locus_tag="VIH70_10255"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEY8116783.1"
                     /translation="MTDQLARAAEALSEAPTIALACHVNPDADALGSMLGLSNHLRGL
                     AKQTVCSFPNEPFEPPRWASMLPGTDALVPPSAFPKDPAVMVTCDVASLDRLASLGGP
                     AVRATTLIWIDHHATNEGLGTIPIIDPSASSTSELVYGLLKVLGGQIRVETALCLYAG
                     LVTDTGRFQYESVGPETLRLAAELREFPFDHTRLVQALYEDHGVAYLKLLGVVLERVR
                     ADPDADLVWTYLTRADLDEADLEPGDADDLIDLVRTAREADVAAILKQQRDGRFKVSL
                     RSRGGHDVAAAAARFGGGGHRLASGFTSSQGLVATVESLKEALDAEPPTV"
     gene            complement(1568..1921)
                     /gene="rbfA"
                     /locus_tag="VIH70_10260"
     CDS             complement(1568..1921)
                     /gene="rbfA"
                     /locus_tag="VIH70_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY8116784.1"
                     /translation="MPEGSRPNRVSEEFREILAEEIPKLKDPRVGFVTVLGVTVTPDL
                     RLARVYYTTLGDERAHRATAAALASARGHLRKVIGSQVRMKVLPELRFEQEDAQGGPD
                     RIEQLLRELHEQEGT"
     gene            complement(1925..2218)
                     /locus_tag="VIH70_10265"
     CDS             complement(1925..2218)
                     /locus_tag="VIH70_10265"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEY8116785.1"
                     /translation="MLVALERFDLRIPACGSLKEKRHVVKTLTSGIRSTFNVSVAEVD
                     HHDLWQRTAIAVAAVAGEQYHARRVLHEVEKLVDRWGEIEVIDRELTLHYPEA"
     gene            complement(2229..>2750)
                     /locus_tag="VIH70_10270"
     CDS             complement(2229..>2750)
                     /locus_tag="VIH70_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEY8116786.1"
                     /translation="RINVIRSAAGAITESDINLALTSRAIVVGFNVRPDVQARDLAEK
                     EGVDVRLYRVIYDAIDDIRAALGGMLKPEERETQLGRAEVRAVFRVPKVGLIAGSYVL
                     EGTIIRNARARVIRDGVVAYDGRIGSLRRFKDDVREVSEGFECGIGIANFNDVKEGDI
                     IEAYEVREVAREL"
BASE COUNT          400 a         1024 c          919 g          407 t
ORIGIN      
        1 cggaccaggt gcgccccgca gccgagggac cgaccgacgt cggcgacgag cacccgcacg
       61 tacgtcccgc ccgagcacgt gacccgcgcc tccagatctc gcccgtcgaa ggccgacacc
      121 gcgaacgcgt cgacccggat cggcctcggc cgcgcctcga gcacctcgcc ccggcgcgcg
      181 gcctcgtaga gcctgcgtcc cccgaccctg acggccgaat aggcgggcgg cgcctgcatc
      241 gactcgccca cgagcgaggc gcaggcgcgt tccacctcgg cgagcgacac gttcaccggg
      301 gccgtccgca cgatctcgcc gtccgcgtcc aacgtgtccg tctcctcgcc cagccgtccg
      361 gtcgccgcgt acgtcttcgg gagctcgccg aggtagcgca gcagccgggt ggcgcgaccg
      421 acgcccagga ccagcagccc cgtggccatc gggtcgaggg tgccggcatg gccgaccttc
      481 cgggtgccga gcgaccggcg cacgacctcg accgcgtcgt gggaggtcat ccccgatggc
      541 ttgtcgacca gcaggaggcc ctccggcgcc ggcgtcatcg acggctagac ggtcggaggc
      601 tcggcgtcga gggcctcctt gagcgactcg accgtggcca ccagtccctg gctcgaggtg
      661 aagcccgacg cgagtcggtg ccccccgccc ccgaagcgcg cggcggcggc cgccacgtcg
      721 tgtccgcccc gagaacgcag gctcaccttg aaccgtccgt cgcgttgctg cttgaggatg
      781 gccgcgacgt ccgcctcgcg cgccgtgcgg accaggtcga tcaggtcgtc ggcgtccccg
      841 ggttccaggt cggcctcatc caggtcggcc cgcgtgaggt acgtccacac gaggtctgcg
      901 tcgggatccg cacggacccg ctcgagcacg acgccgagca gcttcaggta cgcgaccccg
      961 tggtcctcgt agagcgcctg caccagcctc gtgtgatcga acgggaattc gcgtagctcg
     1021 gccgccaggc ggagcgtctc cgggccgacc gactcgtact ggaaccgtcc ggtgtcggtg
     1081 acgagccccg cgtacagaca caacgccgtc tcgaccctga tctggccccc gagcaccttg
     1141 aggaggccgt agacgagctc ggaggtcgac gacgccgacg gatcgatgat cgggatcgtc
     1201 cccaggccct cgttggtggc gtggtggtcg atccagatca gcgtcgtcgc ccgcaccgcg
     1261 ggacccccca gcgacgccag ccggtcgagc gacgccacgt cgcacgtgac catcacggcc
     1321 gggtccttcg ggaacgcgga cggcgggacc agcgcatcgg tcccggggag catcgaggcc
     1381 caccgcgggg gctcgaacgg ctcgttcggg aacgagcaca ccgtctgctt tgcgaggccg
     1441 cgtaggtggt tcgagaggcc cagcatcgat ccgagcgcat ccgcatcggg gttcacgtga
     1501 cacgcgagcg cgatggtcgg agcctccgac aaggcctcgg ccgcccgcgc gagctgatcc
     1561 gtcatcgtca cgtcccctcc tgctcgtgga gctcccggag cagctgctcg atgcggtccg
     1621 gacccccctg cgcatcctcc tgctcgaacc gcagctcggg gagcaccttc atgcgcacct
     1681 gggagccgat caccttacga aggtgcccgc gggccgacgc gagcgcggcg gccgtcgctc
     1741 ggtgcgcacg ctcgtcgccc aacgtcgtgt agtacacgcg cgcgaggcgc aggtcggggg
     1801 tcaccgtgac gccaaggacc gtaacgaacc cgacgcgcgg atccttgagc ttcgggatct
     1861 cctccgcgag gatctcgcgg aactcctccg agactctgtt cggccggctc ccctcgggca
     1921 cgcctcacgc ctccgggtag tgcagcgtga gctcgcggtc gatgacctcg atctcgcccc
     1981 atcggtcgac gagcttctcg acctcgtgca ggacccggcg agcgtggtac tgctcgccgg
     2041 cgacggcggc caccgcgatc gccgtgcgct gccacaggtc gtggtgatcc acctccgcca
     2101 ccgagacgtt gaacgtcgag cggatcccgc tggtcagggt cttcacgacg tgtcgcttct
     2161 ccttgaggga accgcacgcg gggatgcgga ggtcgaaccg ctccagcgca acgagcatcg
     2221 acgtgtccct acagctctcg ggcgacctct cggacctcgt acgcctcgat gatgtcgcct
     2281 tccttgacgt cgttgaagtt cgcgatcccg ataccgcact cgaacccctc gctcacctcg
     2341 cggacgtcgt ccttgaagcg gcggagcgag ccgatccggc cgtcgtacgc gacgacccca
     2401 tcgcggatga cgcgggcccg cgcgttccgg atgatcgtgc cctccaggac gtacgagccg
     2461 gcgatcaggc cgaccttcgg gacgcggaac acggcacgga cctcggcccg tccgagctgt
     2521 gtctcgcgct cctcgggctt gagcatcccg cccagggcgg cgcggatgtc atcgatcgcg
     2581 tcgtagatca cccggtacaa ccggacgtcg acgccttcct tctcggcgag gtcgcgcgcc
     2641 tgcacgtcgg gacggacgtt gaacccgacg acgatcgccc tcgaggtcag cgccaggttg
     2701 atgtcgctct cggtgatcgc gccggcggcg ctccggatca cgttgatccg
//
LOCUS       DATBRK010000344         4982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1221578, whole genome shotgun sequence.
ACCESSION   DATBRK010000344 DATBRK010000000
VERSION     DATBRK010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221578"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VIH70_10275"
     CDS             <1..228
                     /locus_tag="VIH70_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8116787.1"
                     /translation="LRGVADRFETVAMIGHNPGIHDLAIALAGRGTDLDRMRAKFPTG
                     ALAVLAFDGPWRELAPGAARLEAFVTPKDLA"
     gene            225..851
                     /gene="pdxH"
                     /locus_tag="VIH70_10280"
     CDS             225..851
                     /gene="pdxH"
                     /locus_tag="VIH70_10280"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY8116788.1"
                     /translation="MSDVPSHDRPGLTEAELGPDPIARLVAWLDAAGAAGVRFPEAAA
                     LATADAEGRPAVRHVLIKAVDERGLTFFTNHESRKGRHLAENPNAALVLYWRELDRQV
                     SVTGPASRAGDAESLAYFRTRPREARLGAWASRQSRPVGSRRELDAAFAEVSERFSGE
                     DVPLPPHWGGYRLEPKTIEFWQGREHRLHDRFRFTREGGAWRVERLWP"
     gene            937..2280
                     /locus_tag="VIH70_10285"
     CDS             937..2280
                     /locus_tag="VIH70_10285"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8116789.1"
                     /translation="MRADELRVAIAEWHPRAVEELSNLVRIPSIAFPGYDEKPVRECA
                     EATADTLEAAGYGGVRLIELPDVDHPAVFGEVAGPAGAPTILLYAHHDIQPEGPLEEW
                     TSPPFEPEVRGGRLYGRGTSDDKCGIILHAASLRAWGGVPPVSVKVLIEGEEESSAAH
                     LPFLIDGHKELLAADIVAVADGGNWRAGVPTLETSIRGVVDCTVTVRVLDIAVHSGVH
                     GGPAPDALSALARILATVHDDEGEVAIEGLMRGTWDGLQVSEDEFREDTPLRPGVRLI
                     GSGTISDRIWAKPAVSVLGIDAPRVVEASNQLVPWASAKVSLRLAPEDDPDRAMDALT
                     GHLANRAPWGVEVDVKPGSSGRGLRVRTDGPGFAAMTRAMEGAYGKPLVQTGSGGSVP
                     LVPVLSRALPEAEILLYGASDERSQYHSIDESVDLGELERACLGEALLFGELATG"
     gene            2320..2817
                     /locus_tag="VIH70_10290"
     CDS             2320..2817
                     /locus_tag="VIH70_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEY8116790.1"
                     /translation="METASTPDLPQVPTPADSEGGPGAAPEAEHEAITQPAKLLRIAT
                     MVRELLEDTRQASLDEPGRIRLRQIYERAIGELEDVLSPDLQEELSSLAPPMQGVPTD
                     AEIRVAQAQLVGWLEGLFHGIQAALFSQQMAARAQFEELRRRGLPGTSDPQAQGPDQS
                     RGQYL"
     gene            complement(2825..3604)
                     /locus_tag="VIH70_10295"
     CDS             complement(2825..3604)
                     /locus_tag="VIH70_10295"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8116791.1"
                     /translation="MLLVFLYVPLAIIVLYAFNDSVAQAWPIEEFTTKWFGIAWRSPQ
                     VREALANSLEVATIATVFALALGSAAAFAVHRFRFFGRNGVSFLLVLPIALPGIVTAI
                     ALNTTINNFRIPFGVLTIVIGHATFCIVVVYNNVIARLRRTPTSLIEASMDLGADGWQ
                     TFRYVTFPAIRTAIVAGALLAFALSFDEIVVTNFTAGAADTIPRFIYRNLRLPHNRPI
                     VNVVALGVILVMFIPVYIAQRMAGGGDVTAAGAKAQEVAVP"
     gene            complement(3652..4554)
                     /locus_tag="VIH70_10300"
     CDS             complement(3652..4554)
                     /locus_tag="VIH70_10300"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8116792.1"
                     /translation="MVAIAKGQSDESARTRLSAFLFRHPRLKLGLALGGPLSWLLVVY
                     IGSLLFLLANAFWSLDVLTSQIVRGFSFDNFQTLWENDVYRTITIRTVGIAAAVTITD
                     IVLAFPLAFYAARLATPRVRNALLVAVVLPLWANYLVRVFAWKIMLTPNGFVNWLAEL
                     TGLGHLQLGNSNWAVWLTFVYLWLPFTLLPIYAALERVPASYLEASSDLGAKGWTTFR
                     NVLFPLILPGIAAGSIFAFSLTLGDYITPELVGTTQFIGNVIYDNVGVAGNTPLAAAF
                     ALVPIAIMAVYLLGAKRAGAFEAL"
     gene            complement(4620..>4982)
                     /locus_tag="VIH70_10305"
     CDS             complement(4620..>4982)
                     /locus_tag="VIH70_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116793.1"
                     /translation="VTGWADTWMISANAPHPNCMLKWMDWTMQPDVQAEVAVWYGAAG
                     SNTKSCDATRDLLVDFYGKGADEAVDTVRYGNCGNVEFLDSIYLWKTPVPDCGDDRGN
                     ICIDYSEWTQAWTEIQGA"
BASE COUNT          783 a         1742 c         1782 g          675 t
ORIGIN      
        1 ctgcgcggcg tggccgaccg gttcgagacc gtggcgatga tcgggcacaa ccccggcatc
       61 cacgacctcg cgatcgcgct ggccgggaga gggacggacc tggacaggat gcgagcgaag
      121 ttcccgaccg gcgcgctggc cgtgctcgca ttcgacgggc cgtggcggga gctcgcgccg
      181 ggcgctgccc ggctcgaggc gttcgtgacg cccaaggacc tcgcgtgagc gacgtcccgt
      241 cgcacgatcg gcccggcctg acggaagccg agctcggccc cgacccgatc gcccgcctcg
      301 tcgcctggct cgacgcggcg ggggccgcgg gggtccggtt cccggaggcg gccgcgctcg
      361 cgaccgccga cgcggagggg cggccggcgg tccgccacgt gctgatcaag gccgtcgacg
      421 aacgggggct cacgttcttc accaaccacg agagccgcaa gggacgccac ctcgccgaga
      481 acccgaacgc cgcgctcgtc ctctactggc gcgagctcga ccggcaggtg tccgtcacgg
      541 gtccggcgtc ccgcgcgggt gacgccgagt ccctcgcgta cttccggacg cggccgcgag
      601 aggcacggct cggcgcctgg gcctcccggc agagccgccc ggtcggctcg aggcgggagc
      661 tcgacgcggc gttcgccgag gtctcggagc ggttctcggg cgaggacgtc ccgctcccgc
      721 cccactgggg cgggtaccgg ctcgaaccca agacgatcga gttctggcag gggcgcgagc
      781 accgccttca cgaccggttc cgcttcacgc gcgagggcgg ggcctggcgc gtcgaacggc
      841 tctggccctg accactcgag ggcgccgtcg gcggctcgag gctcccccga acgggcgacg
      901 ggtcggacgc tcgcgcgcac gctatcgttc ggccccatgc gcgccgacga gctccgcgtc
      961 gcgatcgccg agtggcaccc tcgcgccgtc gaggagctgt cgaacctggt ccgcatcccg
     1021 tcgatcgcgt tccccggcta cgacgagaag ccggtgcgcg agtgcgccga ggcgaccgcc
     1081 gataccctcg aggcggcggg gtacggcggc gtgcggctga tcgagctccc cgacgtcgac
     1141 cacccggcgg tgttcggaga ggtcgcgggt cccgcgggcg cgccgacgat cctgctctac
     1201 gcgcaccacg acatccagcc cgagggaccg ctcgaggagt ggacgagccc gccgttcgag
     1261 cccgaggttc gcggcggccg tctctacgga cgcggcacgt ccgacgacaa gtgcggcatc
     1321 atcctgcacg ccgcctccct tcgggcgtgg ggcggcgtcc cccccgtgtc ggtgaaggtc
     1381 ctgatcgagg gcgaggagga gtcgagcgcc gcccacctcc ccttcctgat cgacggacac
     1441 aaggagctcc tggcggccga catcgtggcg gtcgcggacg gcggcaactg gcgcgcaggg
     1501 gtccccacgc tcgagacgag catccggggg gtcgtcgact gcacggtcac ggtgcgcgtt
     1561 ctcgacatcg ccgtgcacag cggcgtccac ggcgggccgg cgcccgacgc gttgtcggcg
     1621 ctggcgcgga tcctggccac ggtgcacgac gacgagggcg aggtggcgat cgaggggctc
     1681 atgcggggaa cgtgggacgg cctccaggtg agcgaggacg agttccggga ggacacgccg
     1741 ctgcggcccg gggtccggct catcggctca ggcacgatct ccgaccgcat ctgggccaag
     1801 ccggcggtct cggtcctcgg gatcgacgcg cctcgggtcg tcgaggcctc caaccagctc
     1861 gtcccgtggg cctcggcgaa ggtgagcctg cggctcgcgc ccgaggacga tcccgaccgc
     1921 gcgatggacg cgctgaccgg acacctcgcg aaccgcgcgc cgtggggggt cgaggtcgac
     1981 gtgaagccgg ggagctccgg acgcgggctc cgcgtgcgca ccgacggccc ggggttcgcc
     2041 gcgatgaccc gggcgatgga aggggcctac ggcaagcccc tcgttcagac ggggagcggc
     2101 gggtcggtcc cgctcgtccc cgtgctctcg cgcgccctgc ccgaggccga gatcctgctg
     2161 tacggcgcct cggacgagcg cagccagtac cactcgatcg acgagagcgt ggacctcggc
     2221 gagctggagc gcgcctgcct gggcgaggcg ctgctgttcg gcgagctcgc gacgggctag
     2281 cccgggcgac ttcccgccct cgttgttagt ctcgaaccga tggaaacggc ctcgaccccc
     2341 gacctgcccc aggtgccgac cccggcggac tcggaaggag gcccgggggc cgcgccggaa
     2401 gccgagcacg aggcgatcac gcaacccgcg aagctcctgc ggatcgcgac gatggtcagg
     2461 gagctgctcg aggacactcg tcaggcatcg ctcgacgagc ccggacggat ccggctccgt
     2521 cagatctacg aacgagcgat cggcgagctc gaggacgtgc tctcgcccga cctgcaggag
     2581 gagctctcct cgctcgcccc gccgatgcag ggcgtcccca ccgacgccga gatccgcgtc
     2641 gcgcaggccc agctcgtcgg gtggctcgag ggcctgttcc acgggatcca ggccgcactg
     2701 ttctcgcagc agatggccgc ccgcgcgcag ttcgaagagc tgcgtcgccg tgggctcccg
     2761 ggcaccagcg accctcaggc tcaaggcccg gaccaatccc gcggccagta cctctagcga
     2821 cccgtcaggg aaccgcgacc tcctgagcct tcgcccccgc ggcggtcacg tcgccgccgc
     2881 cagccatccg ctgcgcgatg tagacgggga tgaacatcac gaggatcacc ccgagggcga
     2941 cgacgttcac gatcgggcgg ttgtgcggga gccgcaggtt ccggtagatg aacctcggga
     3001 tcgtgtcggc ggccccggcg gtgaagttcg tcacgacgat ctcgtcgaac gatagggcga
     3061 aggcgagcaa ggccccggcg acgatggcgg tccggatcgc cgggaacgtg acgtaccgga
     3121 acgtctgcca gccgtcggcc cccaggtcca tcgaggcctc gatcagcgag gtaggcgtcc
     3181 ggcgaagccg tgcgatcacg ttgttgtaga cgaccacgat gcagaaggtc gcgtggccga
     3241 tcacgatcgt gagcaccccg aacgggatcc ggaagttgtt gatcgtggtg ttgagcgcga
     3301 tcgcggtcac gatccccggc agcgcgatcg gcaggaccag caggaacgac acaccgttgc
     3361 ggccgaagaa gcggaaccgg tggacggcga acgcggccgc gctcccgagg gcaagggcga
     3421 acacggtcgc gatcgtcgcg acctccagcg agttggcgag cgcctcgcgg acctgggggc
     3481 tacgccatgc gatcccgaac cacttcgtgg tgaactcctc gatcggccac gcctgcgcga
     3541 cgctgtcgtt gaacgcgtag agcacgatga tcgcgagcgg gacgtacagg aacacgagca
     3601 gcatggcggc gctcaccttg atgaggaccc tgaacccgcg ggtctccacg gctacagcgc
     3661 ctcgaacgcg ccggctcgct tggcaccgag caggtagacg gccatgatcg cgatagggac
     3721 gagcgcgaac gccgcggcga gcggcgtgtt cccggcgacg ccgacattgt cgtagatcac
     3781 gttgccgatg aactgcgtgg tccccaccag ctccggcgtg atgtagtcgc cgagcgtgag
     3841 cgagaacgcg aagatcgatc ccgccgcgat gccgggaagg atcagcggga acagcacgtt
     3901 ccggaacgtg gtccagccct tcgcgcccag atccgaggag gcctccaggt aggaggccgg
     3961 cacccgttcc agcgccgcgt agatcggcag gagcgtgaac gggagccaca ggtagacgaa
     4021 ggtcagccag accgcccagt tcgagttccc cagctggagg tggccgagcc ccgtgagctc
     4081 ggcgagccag ttcacgaacc cgttcggcgt gagcatgatc ttccacgcga acacccggac
     4141 caggtagttc gcccagagcg ggaggacgac cgccacgagc agggcgttgc ggacacgcgg
     4201 cgtggcaagt cgggccgcgt aaaacgcgag cgggaacgcg agcacgatgt ccgtgatcgt
     4261 caccgcggcg gcgatgccca ccgtccggat cgtgatcgtt cggtagacgt cgttctccca
     4321 cagggtctgg aagttgtcga acgagaaccc gcgcacgatc tgcgacgtga ggacgtccag
     4381 gctccagaac gcgttcgcga gcaggaacag cagcgagccg atgtagacga cgaggagcca
     4441 gctgagcggg ccgccgagcg cgagcccgag cttcaggcgc ggatggcgga acaggaacgc
     4501 ggacagccgt gttcgagcgc tctcgtccga ctgccccttc gcgatcgcga ccactcgacc
     4561 tccgtggatg cgacgagggc tccgagcgct cgcccgaagc cctcgtcggg ggatcctccc
     4621 tacgcgccct ggatctcggt ccaggcctgc gtccactccg agtagtcgat gcagatgttg
     4681 ccccggtcgt caccgcagtc cggcaccggc gtcttccaca ggtagatcga gtccaggaac
     4741 tcgacgttgc cgcagttgcc gtagcgcacg gtgtccacgg cctcgtctgc gcccttcccg
     4801 tagaagtcga cgagcagatc ccgtgtggcg tcgcacgact tcgtgttgct gccggccgcg
     4861 ccgtaccaga ccgcgacctc cgcctgcacg tcgggttgca tcgtccagtc catccacttc
     4921 agcatgcagt tcgggtgggg ggcgttcgcc gagatcatcc aggtgtccgc ccaacccgtg
     4981 ac
//
LOCUS       DATBRK010000345         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_207614, whole genome shotgun sequence.
ACCESSION   DATBRK010000345 DATBRK010000000
VERSION     DATBRK010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207614"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2643
                     /gene="dnaE"
                     /locus_tag="VIH70_10310"
     CDS             <1..>2643
                     /gene="dnaE"
                     /locus_tag="VIH70_10310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY8116794.1"
                     /translation="AVATNPVRYLVPEDAFLADALECMRELVPINRSNVSRRNAEGWL
                     KPATEMRALFHERPDLVTATLQIAESCAFEIGLHTVRFPDFPTPRGRNASSVLAERSW
                     RGLERRGMRPTARVKDRLHHELAMIHRLGYSAYFLTVADIVADVRAMGIRCACRGSAA
                     GSLVCYLVGISDVDAVRHDLAFERFLNPMRDELPDIDVDVESARREDVYDMVLSRYGD
                     DRCACVAMIDTYRARAAVREVGKALGLPEPEVDVVAKSFPHIGAHDLRVAIERLPELV
                     DSNLNAGQLEQLFRVAERLDGFPRHIALHPSGIVLSNDDLVTRVPLERSFQGYRVIQA
                     DKDDVELLGYLKLDVLGVRMLSSMRHALDEIARTEHVKVDLDEIPLDDEPTFELIRAS
                     DTLGCFQIESPGQRELLQKLQPTRWEDLIVDISLFRPGPVKSDMINPYLRRRAGMEAP
                     TYAHPALRPALRETFGVIVYHEQVLRTLAAMAGYDLTFADHVRRHLDREDLLPGFRAD
                     FLRRATGRGVPADVAERTWTAVAEFASFGFCKAHAAAFAVPTYQSSWLKTHYTAHFLA
                     GILTHDPGMYPRRLLLEDAREHGIPILPLDVNRSEPEYVVEAVSASSRRAEVGAPARA
                     AAAPARGEGVGPGVVERSRSQRLSTSTDHARRRRRAARTEPPTSARHTHVEPPCYGIR
                     LALQDVHGISDAEIRSIMEARAEHPFRDVGDFVRRAAVSKPVVEAIAHAGGFDGIGRG
                     TRRDRLYAAMTTVPQREGDQLALDLSVSAEDRLALPEYTDAERVRAELEVLGLDASRH
                     LVSFFEPLLADLGVVRSRDLHACRSDERVMVAGVKVASQTPAIRSGQRIIFLTLDDAT
                     GPVDVTVFESVQPKVAKTVFHSFVL"
BASE COUNT          382 a          921 c          947 g          393 t
ORIGIN      
        1 gcggtcgcga ccaacccggt ccggtacctc gtcccggagg acgcgttcct ggccgacgcg
       61 ctcgagtgca tgcgcgagct cgtcccgatc aaccggtcca acgtgtcgcg ccgcaacgcg
      121 gaggggtggc tgaagccggc caccgagatg cgggccctgt tccacgagcg acccgacctg
      181 gtgacggcga cgctgcagat cgccgagtcg tgcgcgttcg agatcgggct gcacacggtc
      241 cgcttccccg acttcccgac gccgcggggg cgcaacgcct cctcggtcct ggccgaacga
      301 agctggcgcg gtctcgaacg ccgagggatg cggccgaccg ctcgcgtgaa ggaccggctt
      361 caccacgagc tcgcgatgat ccaccgcctc gggtactcgg cgtacttcct gacggtcgcc
      421 gacatcgtcg ccgacgtgcg ggcgatgggg atccggtgcg catgccgggg gagcgccgcc
      481 ggctcgctcg tctgctacct cgtcggcatc tccgacgtcg acgcggtccg gcacgatctc
      541 gcgttcgagc ggttcctgaa cccgatgcgc gacgagctcc cggacatcga cgtggacgtc
      601 gagtcggcac gccgcgagga cgtctacgac atggtgctct cgcggtacgg cgacgatcgg
      661 tgcgcgtgcg tcgcgatgat cgacacgtac cgcgcccggg ccgccgtccg ggaggtgggc
      721 aaggcgctcg gcctgcccga gcccgaggtc gacgtggtcg ccaagtcctt cccgcacatc
      781 ggcgcccacg acctgcgggt cgcgatcgaa cgcctgcccg agctcgtgga cagcaacctg
      841 aacgcgggcc agctcgagca gctgttccgc gtggccgagc gcctggacgg cttcccgcgc
      901 cacatcgcgc tccacccctc gggcatcgtc ctgtcgaacg acgatctggt gaccagggtt
      961 ccgctggaac gctccttcca ggggtaccgc gtgatccagg ccgacaagga cgacgtcgag
     1021 ctcctcgggt acctgaagct cgacgtgctc ggcgtgcgga tgctctcgtc gatgcgccac
     1081 gccttggacg agatcgcgcg caccgagcac gtgaaggtcg acctcgacga gatcccgctc
     1141 gacgacgagc cgacgttcga gctgatcagg gcctccgaca ccctcggatg cttccagatc
     1201 gagtccccgg gccagcgcga gctcctgcag aagctccagc cgacccggtg ggaggatctg
     1261 atcgtcgaca tctcgctgtt ccggccgggg ccggtcaagt cggacatgat caacccctac
     1321 ctgcgccggc gcgccgggat ggaggcgccg acgtacgcgc acccggcgct gcgaccggcg
     1381 ctccgagaga cgttcggcgt gatcgtctac cacgagcagg tgctccggac cctcgccgcg
     1441 atggcgggct acgacctgac cttcgccgac cacgtccgcc gccacctgga tcgcgaggac
     1501 ctgctcccgg gattccgagc cgacttcctc cggcgggcga ccggacgcgg cgtccccgcc
     1561 gacgtcgccg aacgaacctg gaccgcggtg gccgagttcg cgagcttcgg gttctgcaag
     1621 gcgcacgcgg cggcgttcgc ggtcccgacg taccagtcct cctggctgaa gacgcactac
     1681 acggcgcact tcctcgcggg gatcctcacg cacgaccccg ggatgtaccc gcggcggttg
     1741 ctgctcgagg acgcgcgcga gcacgggatc ccgatcctgc cgcttgacgt gaaccggtcc
     1801 gagccggagt acgtggtcga ggccgtctcg gcgtcgtcga ggcgcgctga ggtcggggca
     1861 ccggcccggg ctgcggccgc gccggcgcgc ggggaggggg tcggtccggg cgtcgtcgag
     1921 cggagccgaa gccaacgttt gtccacgagc acggaccatg cgcgtaggcg gagacgagca
     1981 gcccggaccg agcccccgac ctcagcgcgc cacacgcacg tcgagccccc gtgctacggg
     2041 atccggctcg cgttgcagga cgtgcacggg atctcggacg cggagatccg gtcgatcatg
     2101 gaggctcgag ccgagcaccc gttccgcgac gtgggtgact tcgtgcggcg tgcggccgtg
     2161 tccaagcccg tcgtggaggc gatcgcgcat gcgggggggt tcgatgggat cgggaggggg
     2221 acgcggcggg atcggttgta tgcggcgatg acgaccgtgc cgcagcgcga gggggatcag
     2281 ctggcgctcg acctgtcggt gagcgccgag gatcggctcg cgttgcccga gtacaccgac
     2341 gcggagcggg tgcgggcaga gctcgaggtg ctcgggctgg acgcgagccg gcacctcgtg
     2401 agcttcttcg aaccgctgct cgccgatctc ggcgtggttc gttcgaggga cctgcacgcg
     2461 tgccgctcgg acgagcgcgt gatggttgcc ggcgtgaagg tggcgtcgca gacgccggcg
     2521 atccgcagcg gccagcggat catcttcctc acgctggacg acgcgacggg gccggtcgac
     2581 gtgaccgtgt tcgagagcgt gcagccgaag gtcgccaaga ccgtgttcca ctcgttcgtg
     2641 ctc
//
LOCUS       DATBRK010000346         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2662607, whole genome shotgun sequence.
ACCESSION   DATBRK010000346 DATBRK010000000
VERSION     DATBRK010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2662607"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VIH70_10315"
     CDS             complement(<1..450)
                     /locus_tag="VIH70_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY8116795.1"
                     /translation="MAPTTDFWLRPAERDWRPRPTLSGRDHTSFEVRDEERERIWWGD
                     WVAFGRSEEVAGPGDYVVRDVAGESVFVVRNAEGRLKGFYNVCSHRGTKFLDDDDRGN
                     RRRAFTCPYHAWTYDLDGRLIGTPNVREDEWFDRSSYPLHPIAVDEYA"
     gene            637..1050
                     /locus_tag="VIH70_10320"
     CDS             637..1050
                     /locus_tag="VIH70_10320"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8116796.1"
                     /translation="MDRVPEQRNLVLILARAFAAQLATAVFLLDPEGTIIYYNEAGER
                     LTGRPFIEGAGQTIDEWMMRRRPRDEEGLEVPIQDLPLGTTMLKHDPAHGIITFRTED
                     GVDRRVETASFPLFAHTEDFVGTFSIVWPIEEDRR"
     gene            1047..1889
                     /locus_tag="VIH70_10325"
     CDS             1047..1889
                     /locus_tag="VIH70_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8116797.1"
                     /translation="MKGKIWGSRGSIASPGQDMKRYGGNTSCIELRPPGCDVVILDAG
                     TGIRELGVRLVREGAERMFLLLTHLHVDHLEGLGMFEPIWRKETELHIWGPASPVASL
                     EQRIATYFSPPLFPVHLSEVPATIEYHDASESEWQIDGARFFSNAIIHPGPTVGYRIE
                     SKGRSLAYLTDHEPALGADLDRDPSEWISGFAIAHRTDLLIHDCQYTPQEYEHRVGFG
                     HSTTEHVARFAEKTEVDRLLLFHHDPMHSDEQLDQMREDVVVRWGVDDSRCVVAGEGA
                     ELVV"
     gene            1935..2597
                     /locus_tag="VIH70_10330"
     CDS             1935..2597
                     /locus_tag="VIH70_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234369.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY8116798.1"
                     /translation="MTSEHATASAFLPARLTPGSLERAARVCTGCDLHHLGTQTVFGE
                     GPVPAALMLVGEQPGDREDREGRPFVGPAGALLDRALTEAGVDRDEVYLTNAVKHFRW
                     EGMRGKRRLHRKPSMAQIGACRPWLDAEISIVRPEILVCLGATAAQAILGRSFRLTRH
                     RGELLDAEGLQSKVVATVHPASILRATDDRAREEQRSAFVDDLARAANARRAMHAHDG
                     GG"
     gene            2688..3077
                     /locus_tag="VIH70_10335"
     CDS             2688..3077
                     /locus_tag="VIH70_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116799.1"
                     /translation="MGRVPEPHTSPKAPEDRAWDWHGTEQADKSDAIIESGGWELLAD
                     AHAWFDPSRDEEGHDPPREKGAYKLPHHEIEDGRLRVVWNGVRSAMQVLLGARGGVDI
                     PESDRKDVYLHLAKHYEEFDKEPPPFH"
     gene            3124..>3532
                     /locus_tag="VIH70_10340"
     CDS             3124..>3532
                     /locus_tag="VIH70_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116800.1"
                     /translation="MHGSRITAVVVVGLVAASCTGGGEGPPEAGPARTEAGVPATSQL
                     RMRLVRATGKTQDGTLRPTDIAEPAEAVRRDLEDLYETAFLAPSLDLPALLSHFSGGA
                     RREAERDLGNLTIGPVRGELDEVVPRRATVRLTF"
BASE COUNT          573 a         1232 c         1218 g          509 t
ORIGIN      
        1 cgcgtactcg tcgaccgcga tcgggtgcaa cgggtacgag gaccggtcga accactcgtc
       61 ctcccgcacg ttcggcgtgc cgatcaggcg tccgtccagg tcgtacgtcc acgcgtggta
      121 cggacacgtg aacgccctgc gacggttgcc tcggtcgtcg tcgtccagga acttcgtccc
      181 gcgatgcgag cacacgttgt agaaaccctt caggcggccc tcggcgttgc ggacgacgaa
      241 caccgactcc cccgcgacgt cccgaacgac gtagtcgccg ggacccgcca cctcctccga
      301 tcgcccgaac gcgacccagt ccccccacca gatccgctcg cgctcctcgt cccggacctc
      361 gaagctcgtg tggtcccggc cgctcagggt gggacgaggg cgccagtcgc gttcggcggg
      421 ccggagccag aagtcggtgg tgggcgccat ggtcagttcc gcggtcctct ccaaacggcg
      481 gattgaaccg ccgttcaacg ccggtttcca gagcatacga cggaccgcga ggaaggggaa
      541 ggcggctggt ggatcgacct acccaggagc caggggaggc ggagtctccc cgatcgaacg
      601 gcccggagag ctccaccttc gtactacgct ctgtccgtgg atcgggtccc cgagcagcgc
      661 aacctcgtcc tgatcctcgc tcgggcgttc gccgcgcagc tcgcgacggc cgtgttcctg
      721 ctcgatcccg agggcacgat catctactac aacgaggcgg gcgagcggtt gaccgggcga
      781 ccgttcatcg agggggccgg ccagaccatc gatgaatgga tgatgcgaag gcggccgcgg
      841 gacgaggagg ggctcgaggt gccgatccag gatctcccgc tgggcacgac gatgctgaag
      901 cacgaccccg cgcacgggat catcacgttc cgaaccgaag acggcgtcga tcgcagggtc
      961 gagaccgcgt ccttccccct gttcgcgcac accgaggact tcgtcgggac cttctccatc
     1021 gtgtggccca tcgaggagga ccgccggtga aggggaagat ctgggggagc cgggggtcga
     1081 tcgcctcgcc gggccaggac atgaagcggt acggcgggaa cacatcgtgc atcgagctcc
     1141 ggccgcctgg ctgcgacgtc gtgatcctcg acgcgggcac cgggatccga gagctcggtg
     1201 tccggctcgt gcgcgagggc gccgaacgca tgttcctcct gctcacccac ctgcacgtcg
     1261 accatctcga gggcctcggc atgttcgagc cgatctggcg caaggagacc gagctgcaca
     1321 tctggggtcc cgcatcgccg gtcgcctcgc tcgagcagcg gatcgcgacg tacttctccc
     1381 ccccgctgtt cccggtccac ctctcggagg tgccggccac gatcgagtac cacgacgcgt
     1441 cggagtcgga gtggcagatc gacggcgcgc gcttcttctc gaacgcgatc attcacccgg
     1501 gcccgacggt cggctaccgc atcgagtcca agggtcgctc gctcgcgtac ctcaccgacc
     1561 acgaaccggc cctgggggcc gacctcgacc gcgacccgtc cgaatggatc tccgggttcg
     1621 cgatcgccca tcggaccgac cttctgatcc acgactgcca gtacacgccg caggagtacg
     1681 agcaccgggt ggggttcggg cattcgacga ccgaacatgt cgcgcggttc gccgagaaga
     1741 ccgaagtcga ccggctgctc ctgttccatc acgatccgat gcactccgac gaacagctgg
     1801 accagatgcg cgaggacgtc gtcgtccggt ggggcgtgga cgactcccgc tgcgtcgtcg
     1861 cgggcgaggg cgccgagctc gtggtgtgaa cggccgagga gcgtaaccgc ggagccctcg
     1921 ggtaacaccc tcgcatgacc tccgagcacg ccaccgcctc ggccttcctg cccgccaggc
     1981 tcacgcccgg ctccctggag cgcgcggccc gggtctgcac cggttgcgac ctccaccacc
     2041 tcgggacgca gaccgtcttc ggggagggac ccgtccccgc cgcgttgatg ctggtcggtg
     2101 agcagccggg cgatcgggag gaccgggagg gacgtccgtt cgtgggcccg gcgggagcgc
     2161 tgctcgaccg agccctgacg gaggccggag tggaccgcga cgaggtgtac ctgaccaacg
     2221 cggtcaagca cttccggtgg gaaggcatgc gcgggaagcg ccggctccat cggaagccgt
     2281 cgatggccca gatcggcgcg tgccgtcctt ggctggacgc ggagatctcg atcgtccggc
     2341 cagagatcct ggtctgcctc ggcgcgacgg cagctcaggc gatcctcggg cgttcgttcc
     2401 gcctgacccg gcaccggggg gagctgctcg atgccgaggg attgcaatcc aaggtcgtcg
     2461 cgaccgtgca ccccgcctcg atcctgcgcg ccaccgacga tcgagcacgc gaggagcagc
     2521 gctcggcgtt cgtcgacgac ctcgctcgcg cggcgaacgc ccgacgcgcg atgcacgcgc
     2581 acgacggcgg cggatgatgc gtacgacggc cgtcgaccgg gtacgaagtc tcagggacgc
     2641 aaagcttcgt gacgagcggt cgtagccggc acccggaggt gagggagatg ggtcgagtac
     2701 cggaaccgca cacgagcccg aaggcgcccg aggatcgcgc atgggactgg cacggcacgg
     2761 agcaggccga caagtccgat gcgatcatcg aatccggcgg atgggagctg ctggccgacg
     2821 cacacgcctg gttcgacccc tcacgggatg aggaggggca cgaccctccc cgcgagaagg
     2881 gcgcgtacaa gctcccccat cacgagatcg aggacggacg cctgcgcgtg gtgtggaacg
     2941 gcgtgcgttc ggcgatgcag gtgttgctgg gcgctcgagg cggggtcgac attcccgaga
     3001 gcgatcgcaa ggacgtgtat ctccacctcg cgaagcacta cgaggagttc gacaaggaac
     3061 cgcccccgtt ccactgaacg acgacgggac ccccgcctgc ggtctcgtac cctgtgcggt
     3121 cgcatgcatg ggtcgaggat caccgcggtc gtcgtcgtcg ggctcgtcgc ggcgtcctgc
     3181 acgggcggcg gcgagggacc gccggaggcc ggtcccgcac gaacggaagc aggggtgccg
     3241 gcgacctccc agctgaggat gcggctcgtc agagcgaccg ggaagaccca ggacggcacc
     3301 ctgcggccca cggacatcgc cgaacccgcc gaggccgtcc ggcgagacct cgaggacctg
     3361 tacgagaccg ccttcctcgc accttcgctc gacctgccgg cgctgctctc ccacttctcg
     3421 ggcggcgcgc gccgagaggc cgagcgcgac ctcgggaacc tgacgatcgg acccgtccgg
     3481 ggcgagctcg acgaggtggt cccccggcgc gcgaccgtcc gcctcacgtt cc
//
LOCUS       DATBRK010000347        15326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2663013, whole genome shotgun sequence.
ACCESSION   DATBRK010000347 DATBRK010000000
VERSION     DATBRK010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15326
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2663013"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1615)
                     /gene="hutH"
                     /locus_tag="VIH70_10345"
     CDS             complement(89..1615)
                     /gene="hutH"
                     /locus_tag="VIH70_10345"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY8116801.1"
                     /translation="MPIGVGEPLALEDVVEVALGAEVELGSAASVRIGAARDVIERAV
                     ASNETVYGVTTGFGSLANQRIEPDQADALQLGIVRSHATAVGPPLSREEARAMLLLRA
                     HVLALGHSGVRGEVVERMVEMLNRDLIPVVPEQGSLGASGDLAPLANLALPLVGRGEL
                     LVDGTPRPAGDVLRSAGLEPLQLRAKEGLALVNGTQGMLAIGILAVARAERLVRTADV
                     TAAMSVEAALGSDRVFDERLTALRPHPGVVASASNLRRLLEGSPIVASHRDSTHLVQD
                     AYSLRCSPQVNGATREVLRFVRGVLEVEANAVSDNPVVLPDDDEVLSGGNFHGQPVAV
                     ALDALAAAIVPLASIAERRLYRLLDPTRNNGLPPFLVAESGLNSGFMLVQYTAASLVS
                     ECKTLAHPASVDSIGSSAGQEDHVSMGMTAARHARDVVSNAEVVIALEALVAAQALDL
                     RAPLEPAEATGAARGAIRERVAFLDADRELGADITAVTDLLRTGALLESVEGRLGRLD
                     "
     gene            1682..2182
                     /locus_tag="VIH70_10350"
     CDS             1682..2182
                     /locus_tag="VIH70_10350"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY8116802.1"
                     /translation="MSGPWDDLEPSTPAEGEGRSRSQPGLGPRTPGGFTVFLRGATRR
                     CPRCGSGRLFSSWFKIRDRCPRCGLRLEREEGGFLGAMTLNYLFTAVVWLVVLVVWLA
                     MDLPDLHVPALTVTSIAIAALVPLLFWPFSKTIWASVDFLIYRTSPDYGSREAADRAS
                     GNGGRS"
     gene            complement(2184..3842)
                     /gene="hutU"
                     /locus_tag="VIH70_10355"
     CDS             complement(2184..3842)
                     /gene="hutU"
                     /locus_tag="VIH70_10355"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY8116803.1"
                     /translation="MSAREIRAPRGTELSCRSWPQEAAMRMLMNNLDPDVAERPQDLV
                     VYGGTGKAARSWEAFDAIVRTLRTLGDDETMLVQSGKPVGVFRTHEMAPRVLISNSML
                     VPKWATWDTFRELEHAGLTMYGQMTAGSWIYIGTQGILQGTYETFAEAATQRFGGSLA
                     GTVTLTGGLGGMGGAQPLAVTMNGGVALCVEIDPARIERRIKTRYLDRESDELDEALR
                     WVDEARSAHEAVSIGLVGNCAEVLPQLLARGFAPDLVTDQTSAHDPLGGYVPAGLSPE
                     EAERLRDDDPHGYERRSFESMARHVEAMDGFLDGGAVVFDYGNNLRAGAEAGGLAHER
                     AYAYPGFVPAFVRPMFCEGKGPFRWAALSGDPEDIAKTDRAVAELFPDNERLHRWLEM
                     AEERVAFQGLPARICWLGYGERAEAGLRFNEMVAGGELSAPIVIGRDHLDSGSVASPY
                     RETEAMKDGSDAIADWPILNALVNTAAGAHWVSVHHGGGVGIGYSIHAGMVVVADGSE
                     NATARLERVLTTDPGTGVMRHADAGYEEALAVAADRGIRLPWRE"
     gene            complement(3877..4308)
                     /locus_tag="VIH70_10360"
     CDS             complement(3877..4308)
                     /locus_tag="VIH70_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8116804.1"
                     /translation="MATDQPQAPFHGPAGSSLLVVDDDAFIARLLEIECSAAGYDVRT
                     AGSGDRALELARERCPDLILADVMMPNMDGFELTRRLRMDERTAGAKVILLTARGMSA
                     DRLEGFAVGADDYVIKPFDTTELLARIGEVLAGPRTTRQPA"
     gene            4435..5802
                     /locus_tag="VIH70_10365"
     CDS             4435..5802
                     /locus_tag="VIH70_10365"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEY8116805.1"
                     /translation="MDVLDTVLDAVGDTPLVRLDKVARSVQPAVLAKLEMLNPGGSVK
                     DRIGLRMIETAEREGHLKPGGTIVEPTSGNTGHGLAIAAAIRGYRCIFVMPDKMSQEK
                     ISLLRAYGAEVVICPTAVPPESPESYYRVADRLAEEIPGAYQPNQYFNPENPAAHYDT
                     TGPEIWRQTDGTIDVLVAGVGTGGTITGAARYLKEQKPDVLVVGADPEGSIYSGDVHP
                     YLTEGIGEDFWPTTFDPSLVDRYVRVSDRDAFRMARRITREEGILVGSSSGTAVVATL
                     TVAAELPAETTMVVILPDTGRNYLSKAYSDTWLLQYGLMERPEIVRVEEVLSSKHGHV
                     PPLITVNARDKVRQAIDVLHEFNISQAPVVREDGVDVAQFVGGVHENALLDRIFRDPD
                     ALQADVAEIMGPPIGLVEFDAPIELAFQTLQHGPAVLVEKAGQVLGVLTRSDLLEFLA
                     HRSRV"
     gene            5804..6652
                     /locus_tag="VIH70_10370"
     CDS             5804..6652
                     /locus_tag="VIH70_10370"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEY8116806.1"
                     /translation="MRALGIDVGVRKGLDLVLLDQDRAVLDTARHVGVDDLRKLVPDF
                     GPDVVAIDAPPAWASSGRSRLTERELRWFGIQCFNTPSDPKMAEHPFYEWMTIGFQAF
                     RVIEDAFPRYRSGAVQGSAIEVFPHATAVVLSGFLPPTGMSRHAWRRTVLGAQSVDGA
                     ALRSTDQVDAALAALTGLFALERRFSAPGDPREGKIVLPASTLPARPYRRATTPVRAR
                     EQPPLPGLAPCACGDPACTALTGREFAPGHDAKRKAALWARARQGQEAVDELQRRKWT
                     IPPEMR"
     gene            6670..7809
                     /locus_tag="VIH70_10375"
     CDS             6670..7809
                     /locus_tag="VIH70_10375"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEY8116807.1"
                     /translation="MSEGGMAFETRAIHVGQDPEAEYGSVNVPIYQTSTYAQPAVGKP
                     KAWDYARAGNPTREALQQVLASLEGGSKALCFSSGLGATTTLLLTLKPGDHVLISDDV
                     YGGTYRLLARVMDRWGLVFDSVDMTDLVACRSVVREETALVWVESPTNPFLKVVDIAA
                     VADLAHQAGARCVVDNTFATPYLQRPLELGADVVLHSMTKYLGGHSDLIGGALVSSDD
                     ELAEGLSFLVNAAGAVPGPMDCYLALRGVKTLALRMEAHCRGAKRVAEFLQTHPRVTR
                     VHYPGLPGHPGFEVAVRQMKDFGGMVSFEVDTRDEAIHVAESTELFFLAESLGGVESL
                     IELPGPMTHASVSGSPIEVPDTLIRLSVGIEHPDDLVADLAQALG"
     gene            complement(7856..8995)
                     /locus_tag="VIH70_10380"
     CDS             complement(7856..8995)
                     /locus_tag="VIH70_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8116808.1"
                     /translation="MTGEARILVVDDVPQNVRLLEAILEPRGYDVVSAADGVVALELV
                     ESARPDLVLLDVMMPQLDGYAVCRRLREQDETAVLPVIIVTSSGAEKTKAIQAGADDL
                     IEKPFSHDELLVRVRSLLRIKRYQDTIKAQAAELLELNRTLEERVRHQVLELERLRKL
                     RRFLSPQLADAIVSSGDDSILRSHRRKVAMLFADLRGWTGFVDAVEPEELMQVLGEFH
                     ATIGELVREFDATVGFLEGDGVQLFFNDPEEVPDAAMRAVRLGCALRERMVELTTRWR
                     KRGYELDFGAGIALGYATCGEIGFEGRSDYAAIGSVTNLAARLADEASAGQILIEQRL
                     FAEVEGDVEVRSVGEFTLKGFQRPVTAFDVVAIHRSAANVSVQRS"
     gene            complement(8992..9372)
                     /locus_tag="VIH70_10385"
     CDS             complement(8992..9372)
                     /locus_tag="VIH70_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8116809.1"
                     /translation="MTGERILVVEDNEKNMKLFRDVLVATGYRTLEATTGGEAIELAT
                     AQAPDLVLMDIQMPDVDGVEALRRLRADERTAAIPVLAVTAQAMQGDREHFLAEGFDG
                     YLSKPVNVRELLGTVRQHCDGRVG"
     gene            complement(9369..10898)
                     /locus_tag="VIH70_10390"
     CDS             complement(9369..10898)
                     /locus_tag="VIH70_10390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8116810.1"
                     /translation="MGTSLLLVTVGLFGQRVLGQSNDRVGSLGALQERAFAYGQLQRD
                     AWNVRALLSQTVGNDFYAVWPGADPGDTADPVAADKATLDAAVRLGAMTNADRLGFAP
                     PAEDADTLRSMRTKAELLSALMTPLIRFDEKIVRQLARVHDQGEEPPGSEHWLMLRQR
                     VDRLANDLYQQAAGLANLKTAQIDDLIARNASSFQNSRNLVIGVAVGAIVLALLLGFV
                     LAWSVIGPIQRIDARLAAIASGDFSGHVDVENRDELGGLAANVNRMSDELRRLYTELE
                     ETSRHKSEFLASMSHELRTPLNAIIGFSEVLREEMFGPVNEKQAEYLDDIISSGNHLL
                     SLINDVLDLSKVEAGQVELEVGPFSLREALERGVVMVREQATEEGVQVELSAGPEVDV
                     VEGDERRIKQVIFNLLSNAVKFTPAGGEVDVSAIRVNGEVRVSVADTGPGIALGDHER
                     VFEEFQQTDAGLERLEGTGLGLALSKRLVELHGGRIWLESELGKGSTFVFTLPAGSAS
                     P"
     gene            complement(11041..11544)
                     /locus_tag="VIH70_10395"
     CDS             complement(11041..11544)
                     /locus_tag="VIH70_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8116811.1"
                     /translation="MRKLMIGCAIGVLVVCLAACADSGTSSATEQAQQRKADMWEIDQ
                     IEKDFHQATTTKDIDLMLSLWAPNATLTVGPGQTATGIDEIRSFWLEKSAAFMPVTHW
                     VSDHPAYKLEITLAGDRGTLHFECHYVNYDSDTVDLATVADLDVARIDGRWLITNFVA
                     GSTTLAP"
     gene            complement(11690..12058)
                     /locus_tag="VIH70_10400"
     CDS             complement(11690..12058)
                     /locus_tag="VIH70_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8116812.1"
                     /translation="MPVALIIDDSDANRKLARVVLRADGFETVEAATGAEGIALAIEH
                     VPDVILMDLRLPDIDGREAARRLDENERTSRIPVVALSVMPLEESGEWLEAAGFAGWL
                     AKPIRVGTFPDQVRFYCAGG"
     gene            12076..12741
                     /locus_tag="VIH70_10405"
     CDS             12076..12741
                     /locus_tag="VIH70_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8116813.1"
                     /translation="MPLRVVLAEDHLIVREGVRRLLETQHDVEVAAACGDLDSLLAAV
                     DAEEPDVVLTDIRMPPGNTDEGIQAAKRLRETHPDVGVVVLSQYATPSFALALLEGGS
                     AGRSYLLKERVQDVEQLVSAIRTVAEGGSVIDPKVVEALVAENARGEESRLNQLTPRE
                     REVLREMAAGKSNAAIAESLFLAERSVESVIHSIFLKLGLSWETSVHKRVKAVILYLG
                     EGD"
     gene            complement(12744..13388)
                     /locus_tag="VIH70_10410"
     CDS             complement(12744..13388)
                     /locus_tag="VIH70_10410"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8116814.1"
                     /translation="MKADMLREIEALQAEVGRLRASRRRLAVTTDAERRGLERALHDG
                     VQQQLVGLAANLELAAGSVNADPAAAKKLLAEMGRDIQDAIDQSRKLADRIYPPQLEV
                     GGLVAAVRSAAANANIPARIEIARDQSYPPEVAGAIYFCCLHLLENVSAGSTVAITAR
                     SDGGALAFDIVVDGDLHADRSLLRDRIEALGGRWAIEPESGHQTRVISSLPLSG"
     gene            complement(13538..13753)
                     /locus_tag="VIH70_10415"
     CDS             complement(13538..13753)
                     /locus_tag="VIH70_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116815.1"
                     /translation="MEAIAFHLIAMFSVVCPRADGESLATPNNDATTRQTISTAVGAI
                     AFRNTNVRCMAPPFSPLSRTTVVIPRR"
     gene            13761..14984
                     /locus_tag="VIH70_10420"
     CDS             13761..14984
                     /locus_tag="VIH70_10420"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY8116816.1"
                     /translation="MAAPPTGAGQPPHAAVLTLAMVQGAVYDAVNAIDGGFQPYLAKP
                     SAAPGYSKEAATATAAFRVLVGFPAASPPLAGVVPAAQLSTLQPLYDSSLADVSDGAP
                     KNGGIAVGEASAAAMLTDRLNDGRGGSFKFGQGSEPGEWRLGPPQGPNGIVAQDPAPW
                     VGFVRPFLVPDVDMLRTDGPNSLTSDAYTAEFNEVKELGSLSSTERTADQTAAAIFWQ
                     DSGIAIWNRVYRALALNSGLDIVDSARLFAMTNLAGADASIGCWNDKAYYSFWRPITA
                     IREAANDGNPATIADPSWVPLFDPTVAVSGAPLVTPGFPDHPSGHTCVSSAIVQTLQE
                     FFGTDKVQFTAISNKCSPAPCPARTFDRFSDAIKEIIGARIWSGIHFRTADVQGALLG
                     KKVARYLEKYYFRPV"
     gene            complement(15029..>15326)
                     /locus_tag="VIH70_10425"
     CDS             complement(15029..>15326)
                     /locus_tag="VIH70_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116817.1"
                     /translation="RPDGQTVRWRMAGLDDPRREPWMPFFLAWDVPEDLYPGRARAGH
                     GVWAQGIAWVEVAGNRERLRAWLGDDELPIHVTDGEADIPRVAVGSSDREIVIG"
BASE COUNT         2344 a         5434 c         5179 g         2369 t
ORIGIN      
        1 atcccctact ggaacgcgca gtgggtgtac ggcctcgccg actacggcac ccccgtcgtg
       61 atctaccact gatcgcatcc ggacgcgatc agtccagtcg acccagtctt ccttccaccg
      121 actcgagcaa cgcacccgtg cgcaggagat ccgtcacggc ggtgatgtcc gcaccgagct
      181 cacggtcggc gtcgaggaac gcgacgcgct ctcggatggc accccgagcg gcccccgtcg
      241 cctcggccgg ctcgagcggc gcgcgcaggt cgagcgcctg tgccgccacc aacgcctcga
      301 gggcgatcac gacctcggcg ttggagacca cgtcgcgcgc gtggcgggcc gccgtcatcc
      361 ccatgctcac gtggtcctcc tgcccggcgc tcgaacctat cgaatcgacc gaggcgggat
      421 gggcaagcgt cttgcactcg gacacgagcg acgcggccgt gtactgcacc agcatgaagc
      481 cggagttcag gccgctctcg gcgaccagga acggcggcag cccgttgttc cgcgtcggat
      541 cgagcagccg gtagagcctc cgctcggcga tgctcgcgag cgggacgatc gccgccgcga
      601 gcgcgtcgag cgcgaccgcg accggttggc cgtggaagtt cccacccgag agcacctcgt
      661 cgtcgtccgg cagcacgacc gggttgtccg agaccgcgtt cgcctccacc tcgaggacgc
      721 cgcggacgaa ccggagcacc tcccgcgtcg cgccgttcac ctgcggcgag cagcgcaggg
      781 agtacgcgtc ctgcaccagg tgggtgctgt cgcggtgcga cgcgacgatc ggactcccct
      841 cgagcagccg gcggaggttc gatgcggagg cgacgacccc cgggtgcggc cggagcgccg
      901 tgagccgctc gtcgaagacc cggtccgacc cgagcgcggc ctccacgctc atcgcggccg
      961 tgacgtcggc cgtccggacg agccgttcgg cgcgcgcgac cgcgaggatc ccgatcgcaa
     1021 gcatgccctg cgtcccgttg acgagggcga gtccctcctt ggcccggagc tggagcggtt
     1081 cgagcccggc ggaccgaagg acgtcgccgg cgggccgagg tgttccgtcg acgagcagct
     1141 cgccgcggcc gacgagcggg agcgcgaggt tcgcgagcgg ggccaggtcg cccgacgcgc
     1201 cgagcgaacc ctgctcgggc acgacgggga tcaggtctcg gttcagcatc tcgaccatcc
     1261 gctcgacgac ctcgccgcgg acccccgagt gtccgagcgc gagcacgtgg gctcggagca
     1321 ggagcatcgc gcgcgcctcc tcccgcgaga gcggcggtcc gacggcggtg gcgtgcgagc
     1381 ggacgatccc gagctggagc gcgtcggcct ggtcgggctc gatgcgctgg ttcgcgagcg
     1441 agccgaaccc ggtcgtcacg ccgtagacgg tctcgttcga cgcgacggcg cgttcgatca
     1501 cgtcgcgcgc ggcgccgatc ctgaccgacg ccgcggaacc gagctcgacc tcggcgccga
     1561 gggccacctc gacgacgtcc tcgagcgcga gaggctcccc gaccccgatc ggcacggccc
     1621 aaggctacgg aggggcaccc atcggcggca agccgcgacc cggtcgtacg ctgaccccga
     1681 gatgtcgggg ccgtgggacg atctcgagcc gagcacgcca gcggagggcg agggaaggtc
     1741 gcggagccaa cccggcctgg gacctcgaac gccgggtggc ttcaccgtct tcctgcgagg
     1801 agccaccaga cgctgtcccc gctgcggctc gggacggctc ttctcgagtt ggttcaagat
     1861 ccgcgaccgg tgcccccggt gcggcctcag gctggaacgc gaggagggag ggttcctcgg
     1921 cgcgatgacc ttgaactacc tgttcacggc ggtcgtctgg ctcgtcgtgc tcgtggtgtg
     1981 gctcgccatg gatctcccgg atctgcacgt gccggcgctc accgtcacgt cgatcgcgat
     2041 cgcggcgctc gtgccgctgc tgttctggcc gttctcgaag acgatctggg cgagcgtcga
     2101 cttcctgatc taccggacga gcccggacta cggttcacgg gaggcggccg accgcgcgag
     2161 cggcaacggc ggccgttcct gagctactcg cgccagggga gccgaatgcc gcggtccgcc
     2221 gccaccgcga gcgcctcctc gtagcccgcg tccgcgtgcc gcatcacgcc ggtgcccggg
     2281 tcggtggtca ggacgcgttc gagccgcgcg gtcgcgttct cgctcccgtc ggccacgacg
     2341 accatccccg cgtggatcga gtacccgatc ccgactccgc cgccgtggtg cacgctcacc
     2401 cagtgcgcgc cggcggcggt gttgacgagc gcgttcagga tcggccagtc cgcgatcgcg
     2461 tccgaaccgt ccttcatcgc ctccgtctcg cggtacggcg aggcgacgct cccgctgtcg
     2521 aggtggtcgc gcccgatcac gatcggcgcc gagagctcgc cgcccgcgac catctcgttg
     2581 aaccgcagac ccgcctcggc ccgctcgccg tagccgagcc aacagatgcg cgccggcaac
     2641 ccctggaacg cgacccgctc ctcggccatc tcgagccagc ggtgcagccg ctcgttgtcc
     2701 gggaagagct cggcgaccgc gcggtcggtc ttcgcgatgt cctcggggtc gcccgagagc
     2761 gccgcccaac ggaacgggcc cttgccctcg cagaacatcg gccggacgaa cgccggcacg
     2821 aagcccgggt acgcgtacgc gcgctcgtgc gcgagaccgc cggcctcggc acccgcccgc
     2881 aggttgttcc cgtagtcgaa caccaccgcg ccaccgtcca ggaagccgtc catcgcctcg
     2941 acgtgccgcg ccatggactc gaacgagcgc cgttcgtacc cgtgtggatc gtcgtcgcgc
     3001 agccgctcgg cctcctcggg cgacagcccc gccggcacgt agccgcccag cgggtcgtgc
     3061 gcgctcgtct ggtcggtcac cagatccggc gcgaacccgc gcgcgaggag ctggggcagg
     3121 acctcggcgc agttgccgac gagcccgatc gagaccgcct cgtgggcgga acgagcctcg
     3181 tccacccacc gcagcgcctc gtcgagttcg tcgctctcac ggtcgaggta gcgcgtcttg
     3241 atgcgacgct cgatcctcgc cggatcgatc tcgacgcaga gggcgacgcc cccgttcatc
     3301 gtcacggcga gcggctgcgc gccgcccatc ccgccgagcc cgccggtgag cgtcacggtt
     3361 cccgcgagcg aaccgccgaa ccgctgcgtc gcggcctcgg cgaacgtctc atacgtcccc
     3421 tgcaggatcc cctgggtgcc gatgtagatc cacgagcccg ccgtcatctg cccgtacatc
     3481 gtgagtccgg cgtgctccag ctcccggaac gtgtcccacg tcgcccactt gggcacgagc
     3541 atcgagttcg agatcagcac gcgcggcgcc atctcgtgcg tccggaagac gccgaccggc
     3601 ttgccggact gcacgagcat cgtctcgtcg tcgccgagcg ttcgcagcgt ccgtacgatc
     3661 gcgtcgaacg cctcccacga ccgggcggcc ttccccgtcc cgccgtagac gaccaggtcc
     3721 tgtggacgct ccgcgacgtc ggggtcgagg ttgttcatca gcatccgcat cgcggcctcc
     3781 tgcggccacg agcggcacga gagctcggtg ccgcggggtg ctcggatctc gcgcgcgctc
     3841 acgctcgcga tgataggacg ggatgctcgc ggtggctcag gccggctgcc tggtggtcct
     3901 gggccccgcc aggacctcgc cgatgcgcgc gaggagctcg gtcgtgtcga acggcttgat
     3961 cacgtagtcg tcggcgccga ccgcgaagcc ctccagccgg tcggccgaca tccccctagc
     4021 cgtgagcagg atcaccttgg ccccggcggt gcgttcgtcc atccgcagcc ggcgcgtcag
     4081 ctcgaagccg tccatgttcg gcatcatcac gtcggcaagg atcaggtcgg ggcatcgctc
     4141 ccgggcgagc tcgagcgcgc ggtcgccgct ccccgccgtc cggacgtcgt acccggcggc
     4201 cgagcattcg atctcgagca ggcgggcgat gaacgcgtcg tcgtccacga cgagcaggga
     4261 gctccccgcc ggcccgtgga acggtgcctg gggctggtcg gttgccatca ctcagcaatc
     4321 ggcacatcgg gcgtccgggt gaagctcccg gggacctttc gagacggcgc ctcgggagaa
     4381 cgggagtggc accgttcgtg ggaggatgag gcgacacgac ccctcggagg gtgagtggac
     4441 gtactcgaca cggtgctcga cgcggtcggc gacacgccgc ttgtccgcct cgacaaggtc
     4501 gcccggagcg tgcagccggc cgtcctggcc aagctcgaga tgctcaaccc cggcgggagc
     4561 gtcaaggacc gcatcggcct gcggatgatc gagacggccg aacgcgaggg acacctcaag
     4621 ccgggaggca cgatcgtcga gccgacctcc ggcaacacgg gacacgggct cgcgatcgcc
     4681 gccgcgatcc gcgggtaccg ctgcatcttc gtgatgcccg acaagatgag ccaggagaag
     4741 atctccctcc tgcgggcgta cggcgcagag gtcgtgatct gccccaccgc cgtcccgccg
     4801 gaatccccgg agtcctacta ccgcgtcgcc gaccgcctcg ccgaggagat ccccggcgcg
     4861 taccagccga accagtactt caaccccgag aaccctgccg cgcactacga caccaccggc
     4921 cccgagatct ggcgccagac cgatgggacc atcgacgtcc tggtcgccgg ggtcggcacc
     4981 ggcgggacga tcaccggtgc cgcccgctac ctgaaggagc agaagccgga tgtgctcgtg
     5041 gtcggcgccg atcccgaggg gtcgatctac tcgggcgacg tccacccgta cctgaccgag
     5101 ggcatcggcg aggacttctg gccgacgacg ttcgacccgt cgctcgtcga ccgctacgtg
     5161 cgggtctccg atcgggacgc gttccggatg gcgcgacgga tcacgcggga ggaggggatc
     5221 ctggtgggct cgtcctcggg gacggcggtc gtcgcgacgc tcacggtggc cgccgagctc
     5281 ccggcggaga ccacgatggt cgtgatcctg cccgacacgg gccgcaacta cctctccaag
     5341 gcgtactcgg acacgtggct gctgcagtac ggcctgatgg aacgccccga gatcgtgcgc
     5401 gtcgaggagg tcctgtcgtc caagcacggg cacgtcccgc cgctcatcac ggtcaacgct
     5461 cgcgacaagg tccgccaggc catcgacgtc ctgcacgagt tcaacatctc gcaggccccc
     5521 gtggtccgcg aggacggcgt cgacgtcgcc cagttcgtcg gcggcgtgca cgagaacgcg
     5581 ctcctggacc gcattttccg cgaccccgac gccctgcagg ccgacgtcgc cgagatcatg
     5641 ggaccgccga tcggcctggt cgagttcgac gcgccgatcg agctcgcgtt ccagacgctc
     5701 cagcacggtc cagccgtcct ggtcgagaag gcggggcagg tcctcggcgt cctgaccagg
     5761 agcgacctcc tcgagttcct ggcgcaccgt tcccgcgtct gacgtgcgcg cgctggggat
     5821 cgacgtcggg gttcgcaagg ggctcgacct cgtgctgctc gaccaggacc gggcggtcct
     5881 tgacaccgcg cggcacgtcg gcgtggacga cctgcgcaag ctcgtcccgg atttcggccc
     5941 cgacgtggtg gcgatcgacg cgccgcccgc ctgggcttcc tccggccgtt cccgcctgac
     6001 cgaacgcgag ctccggtggt tcggcatcca gtgcttcaac actccctcgg atccgaagat
     6061 ggcagagcac ccgttctacg agtggatgac gatcggcttc caggcgttcc gcgtgatcga
     6121 ggatgcgttc ccccggtacc gcagcggtgc ggtgcagggt tcggcgatcg aggtgttccc
     6181 gcacgcgacc gcggtggtgc tgtcggggtt cctcccgccg acgggtatga gtcgccacgc
     6241 gtggcggcgg acggtgctgg gcgcgcagtc ggtcgacggg gcggcgctcc gatcgaccga
     6301 ccaggtcgac gccgcgctcg cggccctcac ggggctgttc gcgctcgaac gcaggttcag
     6361 cgcccccggc gatccgaggg agggcaagat cgtgctgccc gcctcgaccc tgcctgcgcg
     6421 cccgtatcgc cgtgccacca cccccgttcg cgcccgcgag cagccgccgc tcccgggact
     6481 cgcaccctgc gcctgcggcg acccggcctg caccgcgctc accggtcgcg agttcgcgcc
     6541 cgggcacgac gccaagcgaa aggcggcgct gtgggcccgc gcccggcagg ggcaggaggc
     6601 ggtcgacgag ctccagcggc ggaagtggac gatcccgccg gagatgcgat gaacgatgcg
     6661 ggagacgcga tgagcgaggg ggggatggcg ttcgagaccc gggcgatcca cgtcggccag
     6721 gaccccgagg cagagtacgg ctcggtcaac gtgcccatct accagacctc gacgtacgcg
     6781 caaccggcgg tcggcaagcc gaaggcgtgg gactacgcgc gggccgggaa cccgacgcgc
     6841 gaggcgctgc agcaggtgct cgcctcgctc gagggcggat cgaaggccct gtgcttctcg
     6901 agcggcctcg gggcgaccac gacgttgctc ctgacgttga agccgggcga ccacgtgctg
     6961 atctcggacg acgtgtatgg cgggacgtac cggttgctcg cgcgggtaat ggatcgatgg
     7021 ggcctcgtgt tcgacagcgt ggacatgacc gacctcgtcg cgtgccgttc ggtcgtacgg
     7081 gaggagacgg cgctcgtgtg ggtcgagtcc ccgacgaacc cctttctgaa ggtggtcgat
     7141 atcgccgccg tagcggatct cgcgcaccag gccggggcgc ggtgcgtcgt ggacaacacc
     7201 ttcgcgaccc cgtacctgca gcgcccgctc gagctcggcg ccgacgtggt gctgcactcg
     7261 atgacgaaat acctcggcgg gcactcggac ctgatcggcg gtgcactcgt ctcgagcgac
     7321 gacgagctgg ccgaaggcct gtcgttcctc gtgaacgcgg ccggcgccgt cccgggtccg
     7381 atggactgct acctcgcgct gcgcggcgtg aagacgctcg ccctacggat ggaggcccat
     7441 tgtcgagggg ccaaacgagt tgcggagttc ctgcagaccc atccgagggt gacccgcgtc
     7501 cactacccgg ggctccccgg ccatcccggg ttcgaggtcg cggtccggca gatgaaggac
     7561 ttcggcggca tggtcagttt cgaggtcgac acgcgcgacg aggcgatcca cgtggcggag
     7621 tccacggagc tgttcttcct cgcggagtca ctcgggggcg tcgagtcgct gatcgagctc
     7681 cccggcccga tgactcatgc gagcgtctcg ggctcgccga tcgaggttcc ggacacgctg
     7741 atccggctct cggtgggcat cgagcacccc gacgacctgg tcgccgatct ggctcaggca
     7801 ctcggctagg aagccgctcc cgtccttgcg acgcacgcct cgaggcagtg tcctgtcagg
     7861 agcgctggac ggagacgttc gcagccgatc gatggatggc gacgacgtcg aaggccgtga
     7921 caggccgctg gaaccccttg agggtgaact cgccgaccga tctcacttcg acgtcgccct
     7981 cgacctcggc aaagaggcgc tgctcgatca agatctggcc ggccgacgct tcatcggcga
     8041 gccgggctgc gaggttcgtc accgacccga tcgccgcgta gtccgagcgg ccctcgaaac
     8101 cgatctcgcc acacgtcgcg taacccagag cgatcccggc gccgaagtcg agctcgtacc
     8161 cgcgcttcct ccaccgtgtc gtcagctcca ccatccgctc gcggagcgcg caccccaatc
     8221 gcaccgcacg catggcggcg tccggaacct cctcggggtc gttgaagaag agctggaccc
     8281 cgtcgccctc gaggaatccg acggtcgcgt cgaactcccg cacgagctca ccgatcgtcg
     8341 cgtggaactc gccgaggacc tgcatcagct cctccggttc gacggcgtcg acgaacccgg
     8401 tccatccgcg gaggtcggcg aacagcatcg cgaccttccg acggtgactg cgcaggatcg
     8461 agtcgtcacc cgacgagacg atggcgtcgg cgagttgcgg cgagaggaac cgccgcagct
     8521 tccgcaaccg ctcgagctca aggacctggt gccgcacgcg ctcttcgagc gttcgattca
     8581 actcgaggag ctccgcggcc tgcgccttga tcgtgtcctg gtagcgcttg atccgaagga
     8641 gcgaccggac tcggacaagc aactcgtcgt ggctgaacgg cttctcgatg agatcgtccg
     8701 caccggcctg gatcgccttg gtcttctccg ccccgctcga tgtaacgatg atcaccggca
     8761 gcacggcggt ctcgtcctgc tcgcggagtc gccggcagac ggcgtagccg tccagctgcg
     8821 gcatcatgac gtcgaggagc acgaggtcgg gcctcgcaga ttccacgagc tcgagggcga
     8881 ccacgccgtc ggcggcggag acgacgtcgt agccgcgcgg ttcgaggatg gcctcgagca
     8941 gccgcacgtt ctggggcacg tcgtccacga cgaggatcct ggcctcgccg gtcatccgac
     9001 gcgtccatcg cagtgctgcc gcacggtgcc gagcagctcg cggacgttca ccggcttcga
     9061 gaggtatccg tcgaagccct ccgccaggaa gtgctcgcgg tctccctgca tcgcctgcgc
     9121 ggtcacggca agcaccggga tggcggccgt gcgctcgtcg gcacgcagcc ggcgaagagc
     9181 ctcgacgccg tcgacgtcgg gcatttggat atccatgagc acgagatccg gcgcttgggc
     9241 cgtcgccagc tcgatcgcct cgccgccggt cgtcgcctcg agcgtccggt agcccgtggc
     9301 gacaaggacg tcgcggaaca gcttcatgtt cttctcgttg tcctcgacca cgaggatccg
     9361 ctcaccggtc acggcgaggc cgatcccgcg ggcagcgtga agacgaacgt gcttcccttg
     9421 ccgagctcgc tctccagcca tatccgtccg ccgtggagtt cgacgagccg cttcgagagc
     9481 gcgagtccga gcccggtccc ctcgagccgc tcgagcccgg catcggtctg ctggaattcc
     9541 tcgaagaccc gctcgtgatc cccgagcgcg atgccgggac cggtgtcggc gacggacacc
     9601 ctcacctcgc cgttgacccg tatcgcgctc acgtcgacct ccccgcctgc cggtgtgaac
     9661 ttcacggcgt tggagagaag gttgaagatg acctgcttga tccgccgctc gtcgccctcg
     9721 acgacatcga cctccgggcc cgcagagagc tcgacctgaa ccccctcctc ggtagcctgc
     9781 tcgcgcacca tcacgacgcc gcgctcgagc gcctcgcgca acgagaacgg cccgacctcg
     9841 agctcgacct gcccggcctc gaccttggac agatccagga cgtcgttgat cagcgagagg
     9901 agatggttcc ccgacgagat gatgtcgtcc aggtactcgg cctgcttctc gttcaccggg
     9961 ccgaacatct cctcgcgcaa cacctccgag aagccgatga tcgcgttcag cggcgtccgc
    10021 agctcgtgcg acatgctggc caggaattcc gacttgtggc gactggtctc ctcgagctct
    10081 gtgtacaggc gccggagctc gtcgctcatc cggttgacgt tggcggccag cccccccagc
    10141 tcgtcgcggt tctcgacgtc cacgtgcccc gagaagtcgc ccgacgcgat cgcggccagc
    10201 cgagcgtcga tccgctggat cggaccgatc accgaccatg cgaggacgaa cccgagaagg
    10261 agcgcaagga cgatcgctcc tactgcgacg ccgatgacca ggttccgcga gttctggaag
    10321 gagctcgcgt tccgcgcgat caggtcatcg atctgcgcgg tcttcagatt ggccaggccg
    10381 gcggcctgtt gatacaggtc gttcgcgagc cgatccaccc gctggcgcag catcagccag
    10441 tgctcgctcc cgggcggctc ctccccttga tcgtggaccc tggcgagctg tctcacgatc
    10501 ttctcgtcga acctgatcaa cggcgtcatc aaggccgaga gaagctccgc cttcgtgcgc
    10561 atcgagcgta gtgtgtccgc gtcctccgcc ggaggtgcga agccgagacg atccgcgttg
    10621 gtcatcgccc cgaggcgaac ggccgcatcc agggtcgcct tgtccgcggc gacgggatcc
    10681 gcggtatccc ccggatcggc cccgggccac acggcgtaga agtcgttccc tacggtctgg
    10741 gagagcaagg cccgaacgtt ccaggcatcg cgctgcagtt gaccgtaggc gaaggcgcgt
    10801 tcctggagcg ccccgaggct tccgacgcga tcgttcgatt ggccgagcac acgctggccg
    10861 aacagtccga ccgtgacgag cagtagggag gtgcccatga acgcgatgag cagcttcgtc
    10921 gagaccttgg ccggaaggcg cccgacggcg cggacgagag gatcgtagcc tcggctcagc
    10981 ggtcgccggc tctcggcgac ggccgggcgc ccccgtcctc cacctgagtc ggacaaggac
    11041 ttagggggcc agtgtggtcg atcccgcaac gaagttggtg atcaaccagc gtccgtcgat
    11101 ccgcgcgacg tccagatcag cgaccgtggc caggtccact gtatcgctgt cgtaattgac
    11161 gtagtggcac tcgaagtgca gggtgccccg gtcgccagcg agcgtgatct cgagcttgta
    11221 cgcgggatgg tccgagaccc agtgcgtcac cggcatgaac gcggccgact tctcgagcca
    11281 gaacgaccgg atctcatcga tgccggtcgc ggtttgcccg ggacccacgg tcagggtcgc
    11341 gttgggcgcc cacaggctca gcatcaggtc gatgtccttc gtcgtcgtgg cctggtggaa
    11401 gtccttctcg atctggtcga tctcccacat gtcggccttg cgctgctgtg cctgctccgt
    11461 cgcagaactc gtgcccgagt ccgcgcatgc ggcaaggcag acgacgagga ccccgatcgc
    11521 gcagccgatc atgagctttc gcatctatga gctccctccg ctcaggctcg cctgggctag
    11581 tcggtccgag gatgcttcca ccgcggctcg catccgtcca gaggcagacg ctagggggag
    11641 cccgtgcccg tgcctaccgt ggtagcacgg aatcccggat gcggctccgt taccctccgg
    11701 cgcaatagaa acgcacctga tccgggaacg taccgacgcg gatcggcttt gcgagccacc
    11761 ctgcgaagcc ggcggcctcg agccattcgc cgctttcctc cagcggcatc acgctcaacg
    11821 cgactaccgg gatccgagac gttcgctcgt tctcatcgag acgccgcgcc gcctcccggc
    11881 cgtcgatgtc gggcaggcgg aggtccatga ggatgacatc gggtacgtgc tcgatggcga
    11941 gcgcgatccc ctcggcgccc gttgccgcct cgacggtctc gaatccgtcc gcgcgaagga
    12001 ccacccgagc aagcttccgg ttggcatcgc tgtcgtcgat gatcagggcg acgggcacgg
    12061 gcgtatcctc cggaaatgcc gctccgggtc gtcctcgccg aggatcacct gatcgtccgc
    12121 gagggcgttc ggcggctgct ggagacgcag cacgacgtcg aggttgcggc agcgtgcggc
    12181 gacctggact ccctgctcgc cgcggtcgac gccgaggaac cggacgtggt cctcacggac
    12241 atccgcatgc cgccgggaaa caccgacgag gggatccagg cggcgaagcg attgcgtgag
    12301 acgcatcccg acgtgggcgt cgtcgtgctc agccagtacg ccacccccag cttcgccctg
    12361 gccctgctcg agggcggcag cgccggtcgt tcctacctcc tgaaggagcg cgtccaggac
    12421 gtcgagcagc tcgtgtcggc catccggacc gtcgcggagg gcggctcggt gatcgacccg
    12481 aaggtcgtcg aagccttggt cgcggagaac gcccgtggcg aggaatcgcg cctgaatcag
    12541 ttgacgccgc gcgagcgcga ggtgctgcgg gagatggcgg ccggcaagag caacgcggcg
    12601 atcgcggaat cgctcttcct cgccgagcga tcggtcgaga gtgtgatcca ctcgatcttc
    12661 ctcaagctcg gcctttcctg ggagacgtcg gtccacaagc gggtcaaggc cgtgatcctg
    12721 tatctcgggg agggcgacta acgtcatccc gagaggggca gcgagctgat cacccgcgtc
    12781 tggtgaccgg actccggctc gatcgcccat cggcctccga gagcctcgat gcgatcgcgg
    12841 agcagggagc ggtccgcgtg gaggtcgccg tccacgacga tgtcgaacgc gagcgctccc
    12901 ccatcgctcc gtgccgtgat cgccaccgtc gagccggccg agacgttctc gagcaagtgg
    12961 aggcagcaga agtagatcgc ccccgcgacc tcgggcggat aggactggtc gcgagcgatc
    13021 tcgatccgcg cagggatgtt cgcgttagcc gccgccgagc gcaccgccgc aaccagacct
    13081 cccacctcca gctgcggggg ataaatgcgg tccgcaagct tgcgcgactg atcgatcgcg
    13141 tcctggatgt cgcggcccat ctctgcgagc agcttcttcg cggccgccgg gtcggcgtta
    13201 accgaacccg ccgcgagctc gaggttggcg gcgagcccca ccagctgctg ttgcacgccg
    13261 tcgtgcaacg cccgctcgag gccacggcgc tcggcgtcgg tggtcacggc gagccgtcta
    13321 cgcgaggcgc gtagcctccc gacctcggcc tgcaacgcct cgatctctcg cagcatgtcg
    13381 gccttcaccg tcctcagtcg atcgacctcc tacgcaggac tcaccggaag ttgaccgtta
    13441 tcggtcgggc gcgtcaagcg caataccgtg ctgccacggg ggtgctggac ccacgcattt
    13501 ggcgagccgc ccccgtagag cgagccgagc cgtcctccta gcgtcgcggg atgacgaccg
    13561 tcgttcggga gagcggcgag aagggagggg ccatgcaacg cacgttcgtg ttgcggaagg
    13621 cgatggcacc gacggcggtg ctgatcgtct gcctcgtggt tgcgtcgttg ttcggcgtcg
    13681 cgaggctttc accgtctgcg cgcgggcaga cgaccgagaa catggcgatc agatggaacg
    13741 cgatcgcctc caccgcgatc atggcggcac cgccgacggg ggcgggtcag cccccccatg
    13801 cggccgtact cactctcgcc atggtgcagg gcgccgtcta tgacgcggtc aacgccatcg
    13861 atggcggctt ccagccgtat ctggccaagc catccgcggc tccgggttac tcgaaggagg
    13921 ccgcgaccgc gaccgccgcg ttccgggtcc tcgtcggctt ccccgctgct tcgcccccgc
    13981 tggcaggtgt ggtcccggcg gcgcagcttt cgacgctgca gccgctctat gactcgtcgc
    14041 tcgctgacgt gtccgatgga gcgccgaaga atggtgggat cgcggtcggc gaagcgtccg
    14101 ctgccgccat gctgaccgac cggttgaatg acggccgtgg cggctccttc aagtttgggc
    14161 agggcagcga gcctggggag tggcggctcg ggccgccgca aggtccgaac gggatcgtcg
    14221 ctcaggatcc agctccgtgg gtgggcttcg tccggccgtt cctcgtcccg gacgtcgaca
    14281 tgctgcgcac ggacggtccg aactcgctca ccagtgatgc ctacacagcg gaattcaacg
    14341 aggtcaagga actaggttcg ctctccagca cggagcgcac ggcggatcag acggcggcag
    14401 cgatcttctg gcaggacagc gggatcgcga tctggaaccg cgtctaccgg gctctcgcgc
    14461 tgaacagtgg gctcgacatc gtcgacagcg cacgcctgtt cgcgatgacc aacctggccg
    14521 gagccgacgc gtcgatcgga tgctggaacg acaaggcgta ttacagcttc tggaggccga
    14581 tcaccgcgat ccgcgaggcg gcgaacgacg ggaacccggc gaccatagcc gatccgtctt
    14641 gggtcccgct cttcgacccg accgtcgcgg tgtccggcgc accactggtc actcccgggt
    14701 tcccagacca cccctcgggg cacacctgcg tcagcagcgc gatcgtgcag acgctccagg
    14761 agttcttcgg gaccgacaag gtccagttca cggcgatcag caacaagtgc tcccccgcgc
    14821 cgtgtccggc caggacattc gaccgcttct cggacgcgat caaggagatc atcggagcca
    14881 ggatctggag cggcatccac ttccgaacgg cggacgtaca gggcgccttg cttggcaaga
    14941 aggtcgcccg ctacctggag aagtactact tccggccggt ctgaagcatc aacggtgagg
    15001 gcctcgcgtt gtgcgaggcc ctcgctcgct acccgatcac gatctcccgg tctgagctcc
    15061 cgacggccac acgagggatg tccgcttcgc cgtcggtcac atggatcggg agctcgtcgt
    15121 ccccgagcca cgcgcggaga cgctcccggt tcccggcgac ctccacccac gcgatgccct
    15181 gcgcccagac gccgtggcca gcccgagcgc gaccggggta gaggtcctcc ggcacgtccc
    15241 acgcgaggaa gaacggcatc caaggctcgc gccgggggtc gtcgagcccg gccatccgcc
    15301 agcgaacggt ctggccgtcc gggcgc
//
LOCUS       DATBRK010000348         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_783593, whole genome shotgun sequence.
ACCESSION   DATBRK010000348 DATBRK010000000
VERSION     DATBRK010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783593"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="VIH70_10430"
     CDS             <1..1552
                     /locus_tag="VIH70_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8116818.1"
                     /translation="QIYVRSFADSDGDGTGDLQGVIEHLDHLEWLGVDGIWLSPTTPS
                     PDRDWGYDVSDYWDVHPALGELATLDRLVSEAASRDIRVILDLVPNHTSDRHPWFVDA
                     RSSREAAHRDWYVWADPRADGSPPNNWRSVFGGSAWELDPDTGQYYLHNFLPEQPDLN
                     WWNEEVRDEFDVILRFWFDRGIAGFRIDVANGLIHDPQLRDNPPATADDPPEIRRRGH
                     RQVYNLNRPEVHDIYRGWRTIADDYEPKRILVAETWVFEPRELARYYGTGDDELHLCF
                     NFSLVFSEFGAEELGAVVGSTEAALPPGSQPAWTGSNHDVGRFPTRWCDGWGERSRCA
                     LMALLALRGTPFLYYGDEIGMIDVPIPEDAQLDLVGGGGAEQPSRDAGRTPMRWSGRP
                     GGGFTREGVRPWLPIGDPRAINVEDQRGDPGSMLQLCRDLIVLRRETPDLRSGDYASL
                     NSPDGAWAWRRGDSIVVAVNLSGEAASLPLGPATIEIGTDRARDGETVEGDLGLGPWA
                     GAVLRLGA"
     gene            complement(1563..>1920)
                     /locus_tag="VIH70_10435"
     CDS             complement(1563..>1920)
                     /locus_tag="VIH70_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116819.1"
                     /translation="VFLALAFLSQHPRAPRATVWAMLLCIVVGIPVSAIAADAVTGFV
                     AGVGAGGIAAMRSDLDRAWRARALAIVIVSAWAFVTVRTVTDVALLLAPVLPFTSLGV
                     ADHLVEIRREGKVGPS"
BASE COUNT          307 a          656 c          681 g          276 t
ORIGIN      
        1 ccagatctac gtccgatcct tcgccgacag cgacggcgat ggcaccggcg acctgcaggg
       61 cgtgatcgaa cacctcgacc acctcgagtg gctcggcgtg gacgggatct ggctctcccc
      121 caccacgccg tccccggatc gggattgggg ctacgacgtc tccgactact gggacgtgca
      181 tccggcgctc ggcgagctcg cgacgctcga ccggctggtc tcggaagcgg cttcccggga
      241 catcagggtg atcctcgacc tcgtgccgaa ccacaccagc gaccggcatc cgtggttcgt
      301 cgacgctcgg tcctcgcggg aggccgcgca tcgcgactgg tatgtgtggg cggatccacg
      361 cgccgacgga tccccaccca acaactggag gtcggtcttc gggggaagcg catgggagct
      421 cgaccccgac acgggtcagt actacctcca caacttcctc cctgagcagc ccgatctcaa
      481 ctggtggaac gaggaggtac gagacgagtt cgacgtcatc ctgcggttct ggttcgaccg
      541 gggcatcgcg ggtttccgga tcgacgtcgc caacggactg atccatgacc cgcagctgcg
      601 cgacaaccct ccggcgacgg ccgacgaccc gccggagatc cgtcggcggg gtcatcggca
      661 ggtctacaac ctgaatcgtc cggaggtgca cgacatctat cggggctggc ggacgatcgc
      721 cgatgactac gagccgaaac ggatcctcgt cgccgagaca tgggtcttcg agccccggga
      781 gctcgcgcgt tactacggaa cgggtgacga cgagcttcac ctctgcttca acttctcgtt
      841 ggtcttctcc gagttcgggg ccgaggagct cggggcggtc gtgggatcca cggaggcggc
      901 gctcccaccc ggatcgcaac ccgcctggac ggggtcgaac cacgacgtcg gccggttccc
      961 gacgagatgg tgcgacggat ggggtgagcg gtcccggtgc gcgctcatgg cgctgctggc
     1021 gcttcgaggg acacccttcc tctactacgg ggacgagatc ggcatgatcg atgttccgat
     1081 accggaggac gctcagctcg acctggtcgg cggaggggga gccgaacagc cgagccgcga
     1141 cgccggacgg accccgatgc ggtggagcgg gcggccgggg ggtgggttca cgcgcgaagg
     1201 cgttcggccg tggctcccga tcggcgaccc gcgcgcgatc aacgtcgagg atcagcgggg
     1261 ggacccgggt tcgatgctcc agctgtgccg cgacctcatc gtcctgcgcc gcgagacgcc
     1321 ggacctgcgt tcgggcgact acgcctcgtt gaactcgccc gacggggcgt gggcctggcg
     1381 gcgcggcgac agcatcgtcg tcgcggtcaa cctgtcgggc gaggcggcct cgctccccct
     1441 cggcccggcc acgatcgaga tcggaaccga tcgcgcgcgc gacggcgaga cggtggaggg
     1501 cgatctcggt ctggggccgt gggcgggggc ggtgctgcgg ctgggcgcct gagcgtccgg
     1561 actcaggacg gtccgacctt cccctcgcgc cggatctcga cgagatggtc cgcgactccc
     1621 aggctcgtga acggcaggac cggcgccagc agcagggcga cgtcggtcac cgttcgaacc
     1681 gtcacgaagg cccaggccga gacgatcacg atcgcgagcg cccgtgctcg ccaggcgcga
     1741 tcgagatcgg agcgcatcgc cgcgatcccc cccgccccca cgccggcgac gaacccggtg
     1801 acggcatcgg ccgcgatcgc ggacaccggg atccccacga cgatgcacag gagcatcgcc
     1861 cagaccgtcg cccgaggggc acgcgggtgc tgggacagga acgcgagcgc gaggaagacg
//
LOCUS       DATBRK010000349         5894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_65345, whole genome shotgun sequence.
ACCESSION   DATBRK010000349 DATBRK010000000
VERSION     DATBRK010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65345"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VIH70_10440"
     CDS             complement(<1..613)
                     /locus_tag="VIH70_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEY8116820.1"
                     /translation="MTLAVRWYLRFGLSYRYVEELLGERGIEVDHVTIYRWVQRFAPE
                     FAEAARTRQHLVGDRWHVDETYLKVGGTWRYLYRAIDQFGQVIDVFLSPRRNKEAARR
                     FFAQAISRTRVSPTEVTTDRYRVYPRVLGELPPAAFHHTEAHANNPLETDHGRLKARL
                     RPMRGLKRDRTARVIVGGHAFVQNLRRGFYDLEDRCPTHGSPRR"
     gene            complement(1916..2308)
                     /locus_tag="VIH70_10445"
     CDS             complement(1916..2308)
                     /locus_tag="VIH70_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8116821.1"
                     /translation="MATLTTVEEVMSTELIAVNPSANLMEAAGAMSAGGAGSVLVIQD
                     GALVGIFTERDILRALGYSSSADLARVSSVSKWMSRDPMTIGPDTTVAEALNRMLFGG
                     FRHLPVMDGDAVVGMVSMRDLARSIAEH"
     gene            2686..2913
                     /locus_tag="VIH70_10450"
     CDS             2686..2913
                     /locus_tag="VIH70_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116822.1"
                     /translation="MEEFHKQLEGADVDLLLEMVKVFAEQLGAEADALAQCVCLRTGD
                     RAPKQISAPAFRMTLGALARYLPGLSGIRFL"
     gene            2980..3912
                     /locus_tag="VIH70_10455"
     CDS             2980..3912
                     /locus_tag="VIH70_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116823.1"
                     /translation="MTGTLATHAVIVSATLTTPRATRCLRSLSVTLLACVVLTMKLLV
                     LVTDPAGVVTLIGPVVAPAGTVAVILVDLTTLNVAAVPLKVTPVVPVKFVPVMVTVVP
                     TGPKVGVNEVIVGAPAAVVTLKPWELQSLPPGVVTQIFPVVAPLGTVAAIFVEDSTVN
                     VAETPWNVTLVAPVKFVPVIVTVVPSCPEVGEKEVIVGGAEAVTVKLWELVAVPSGVV
                     TRIGPVVAPEGTVVVILMFEFAVNVADTPLNVTLVDAMRSVPVIVTDVPTWPLVGEND
                     EIVGAAAQDAGAIAIAATMTPVTIAARASVALLG"
     gene            complement(4695..5519)
                     /locus_tag="VIH70_10460"
     CDS             complement(4695..5519)
                     /locus_tag="VIH70_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116824.1"
                     /translation="MAVAASRRWGVIVGGALAVAAVWIIATGGPFARTEAPTASGPTV
                     PPALRFMPAFPSVSDRVSLTGLPPDGAVPSTPKTGEVIAQYHEFRTTTASSSTLVGWV
                     YVYADGRVIWSSVEGWMYERRLTSVGLSSVRSRAARPRVFLEKNETSQLPEGAWADRI
                     RVYVPSRYAICYFGRRGRRLDASRTVGLFPASVEALLRGKEWTYDVAQRFAHDPAECS
                     RVTTEDARSILEILRDRGGTGEVNGGWGYRFAPSETAWDEVSLTFHPLLPQGTWVE"
BASE COUNT          989 a         1898 c         1915 g         1092 t
ORIGIN      
        1 gtcggcgagg cgagccgtgg gtcggacatc ggtcctcgag gtcgtagaag ccccggcgga
       61 ggttctggac gaaggcgtgc ccgccgacga tcaccctcgc agttcggtca cgcttcagcc
      121 ctcgcatcgg tcggaggcgc gccttgagac gcccgtggtc ggtctcgagc ggattgttcg
      181 catgggcctc cgtgtggtga aaggccgccg ggggcagctc acccaggacg cgagggtaga
      241 cccggtaccg gtcggtggtg acctctgtag gcgagacgcg agtccggctg atcgcctggg
      301 cgaagaacct ccttgcggcc tctttgttcc gccgtggcga gaggaagacg tcgatgacct
      361 gcccgaactg gtcgatggcc cggtacaggt agcgccaggt cccgccgacc ttcaggtatg
      421 tctcatcgac gtgccagcga tccccgacca ggtgctgtcg agttcgtgcg gcctcggcga
      481 actcgggagc gaagcgttgg acccatcggt agatcgtgac gtggtcgacc tcgatcccgc
      541 gctcacccag gagttcctcg acgtaccgat aggacaggcc gaaccgcagg taccagcgga
      601 ccgccagggt catccgtgac tttgcgagat cccgatcgcg agtgaacgtg accacccagc
      661 gctcgtcgtc gccgacatac gtgtagcgcg tggttgtcgc gatcggcttg cctgtcaatt
      721 cgtccgtgta agtgtcgagc gcttcgaacc gcacgcgtcg cgagtcatct gcgaccactt
      781 cgccgtcgcg cgcgagcgtg acgatcggta gttccgtgta gtcgtatcgc ctgtccgcgg
      841 tgatgagcga cgcgatcacc gtgtacggac cggcctgccc acgcgcccag taccagtcgt
      901 ggacgatctt ctgaaggccg acgttgcccc agttgtggtc gtggtatccg acgccggtgg
      961 tctcgtgcgc ctcgtcgcca atgcgatagg tggcggtaac cgcaccccgg gggaccgatg
     1021 gcagccaagc gaactccttg tcgcgttcgg tcccgtacag gatgtaaccg gtctgcggcc
     1081 gccatgcccg tacctcgccg acaagtgtga cgtcgacgcc gatctcttca acggtggcct
     1141 ggatccgata tgcgtgaagg tcgccgctga accggttttc cccgatgcgc acgtcggcgc
     1201 tcgtggtgga ggcctcccag ctttcgggcg cgtaggtgac gagcttgtgg aagctgcgtc
     1261 cgtccgctaa gtccaggtcg atccggatcg tcggcgtgag cggatcgttc ggggtagctt
     1321 ggttcttatt catgaagacg acgacgagat tggagccgtc gtcgagatgg gagtcgaagt
     1381 accaccactc gtaggttccg cgccggttgt cggtgcgagc ggcgtcctcc cacggcgcca
     1441 cagagtcgcg tgccaacttg agccgctcat agtcgcgatc ctccgatgcg atgactgcga
     1501 gtggcctgtt cttttgcggc atcggtgaac tcatgttcgt cctttcgcct gaggaagggt
     1561 aggcggtcag tgggcggtcc tcatcgccga gcaaggggcc cgtcgttgcg gctgcacgtg
     1621 cccgggttga gctctcgagt ccctcccacc atcatcatcg cgaacacagg ctcaagactg
     1681 gccatcctgg gaacctcgca tcgggagcta ctcggcgcca tcatcaaggc ccagcaggcg
     1741 gcgagaaggt ggccctatct cgcgtggtcg acgtgacagt ctgcctaacc gatggaccca
     1801 ttgatgattc cgagcagttg ctccccttgc gccgcgacgg actccagagc cgcggacgtt
     1861 cggtcgcctc gccggatctg tatccagtcc atgaggtttc cggggcggcg aggactcaat
     1921 gctcggcaat gctcctggca agatcacgca tcgagaccat ccccaccacc gcatcgccat
     1981 ccatcacggg gagatgccgg aatcccccga acagcatccg gttgagcgcc tcagccaccg
     2041 tggtgtcggg tccgatcgtc atcggatctc gggacatcca cttggagacc gatgagaccc
     2101 tggccagatc cgcgctggag gagtacccca gcgcccggag gatgtcgcgc tcagtgaaga
     2161 tgcccacaag ggcgccgtct tgaattacaa gcacggagcc cgcgcccccc gcggacatgg
     2221 caccggcggc ctccatgagg ttggcggagg gattgaccgc gatgagctct gtgctcatga
     2281 cttcttcgac ggtcgtcaac gtcgccacca ggctcgggtc ggcagctagg tacctcttcg
     2341 tacctcttca tacgtatcac cccggtcaag ttccaatcct gagagcaccg ggatctcgag
     2401 ccgcaaggcg agtcgagcct ccagggccct tttcgcgtcc cctcgcccaa aggcacaatt
     2461 cgctaacgca gggctatgtg ggtctccctc cctccctggc atgccattac tcaccccagt
     2521 cagttcatgc caagccgacg caagtcgttc agtagggtcc gcggacgtgg tgtacgaacc
     2581 acgatctgaa ggtggctgaa caggaagacc accgcgtcat cctcaacgag atctgtggga
     2641 tccccgagag gaggagcgcg gtgggtcggg aacaggattg acgtcttgga ggagttccac
     2701 aagcagctcg aaggagcaga tgtcgacctg ctgctggaga tggtgaaggt cttcgcagag
     2761 cagctggggg ccgaagccga cgcgctagct cagtgcgtgt gcctccggac gggcgaccgg
     2821 gcgcccaaac agatatcggc tcccgcgttc agaatgaccc tcggggcctt ggcccgatac
     2881 ctgccgggct tgtcggggat ccgcttcttg tagatccccg tcgaggtggt ggtggtcctg
     2941 cctaccgtct tccactggcc cgacacgcga cgctgaacct tgacgggaac gctcgccaca
     3001 cacgccgtca tcgtgtccgc aacgctgacg actccacgcg cgaccaggtg cctccgtagc
     3061 ctcagtgtca cactccttgc atgcgtagtg ctgacgatga agttgctcgt gctggtcacc
     3121 gacccggcag gcgttgtcac cttgatcggg ccggtcgttg caccagccgg gacggtcgcc
     3181 gtgatcttgg ttgacttgac caccttgaac gtcgcagcgg tgccgttgaa ggtcacgccc
     3241 gtcgtcccgg tgaagttcgt cccggtgatg gtaacggtcg ttccgaccgg cccgaaggtc
     3301 ggcgtgaacg aggtgatcgt gggggccccg gcggcggtcg tgaccttgaa gccctgggag
     3361 ctgcagtctc ttccaccagg cgtggtcacg cagatcttcc ccgtcgtcgc tccgcttggg
     3421 accgttgccg cgatcttcgt cgaggattcc acggtgaacg tggcggaaac gccgtggaac
     3481 gttaccttgg tcgccccggt gaagttcgtc ccggtaatcg tgaccgtcgt tccctcctgc
     3541 ccggaggtgg gcgagaagga ggtgatcgtc ggaggcgcgg aggccgtgac ggtgaagctc
     3601 tgggagctgg tcgccgtccc ttcgggggtg gtcacgcgga tcggccccgt ggtcgcacct
     3661 gagggaaccg tcgtagtgat actcatgttc gagttcgcgg tgaacgtggc ggacactccg
     3721 ttgaacgtca cgctcgtcga tgccatgagg tcggttccgg tgatcgtcac cgacgtgccc
     3781 acctggccgc tggtgggcga gaacgatgag atcgtgggcg cagcggccca ggacgcgggt
     3841 gcgatcgcca tcgcggcgac catgaccccg gtgacgattg cggctcgggc gagcgtcgcc
     3901 ttgctcggat gagacacggc tgcctcctct caacgcctta cagcgatgtt catacccacg
     3961 atcgtacgga gtgtgcatca gcccttttgg tctaagtccc acggcaccct taacacagcg
     4021 gtcgagccgg cagcacccgc tgaggtgaat ggcacacaaa ccaccaatcg gccctcgacc
     4081 agcgccttcg taccatcggg gctcgttctc gtgggagtta gtccgcgcga ggctgcctcg
     4141 ctggtagcca cccagatcct cgatcggtga gtcgagcttc atcaaggcgc tcgcctgctc
     4201 gccattcggc atcaacacgg cagcgccctc ggggcaggca ttcccaaaga cgaaccctca
     4261 gccgacgtgg acgagccggt ccccgtcgag gacggtgatg tcgccgtagt gcagaccgcg
     4321 agcggcgccg aggtgggaca ggagttcctg aagatggaga ggttgcggcc gcgatactcg
     4381 ccggagccgg catctgggcc ggcgcaactt gggccatcat ccggagcggg actgagtggc
     4441 gatggattgc agtggcgatt gccgtcgaaa gcggtcatcg tggcggcgcc attgtcattc
     4501 ctagactccg gccgacgatg ctgctcggcg cgggagtagc cgtattcatg acagccgccg
     4561 ggtcgtgttc caactgccac gaaagtggtg cttcgcatat aactggagcg gcgggcctcc
     4621 atccggttgc agggatttcc gctgtggggc ctatccgcgt ggtgggtgtt ggactccgat
     4681 cgaaccggtg aggctcattc gacccacgtc ccttgcggga ggagcggatg gaacgtgagc
     4741 gagacttcgt cccaggccgt ctccgagggc gcgaagcggt acccccaccc cccgttaacc
     4801 tccccggtgc ctcctctatc gcggaggatc tcgaggatcg agcgggcgtc ctccgtcgtc
     4861 accctggagc attcggcggg atcgtgggcg aagcgctgag caacgtcgta cgtccattcc
     4921 ttgcccctga ggagagcctc gaccgacgcc ggaaacaggc ccacggtgcg ggacgcgtcc
     4981 agtcgtcgtc cccgtcgacc gaaatagcag atggcgtacc tggacggcac gtacaccctg
     5041 atccggtccg cccacgcgcc ctccggcagc tgagatgtct cgttcttttc gaggaacacc
     5101 ctcggcctcg cggcacggga ccgcacgcta gagaggccaa cggacgtgag acgtcgctcg
     5161 tacatccaac cctctactga ggaccagatc acccgtccgt cggcgtacac gtagacccag
     5221 ccgacaagcg tcgaagaact tgctgtcgtt gttcgaaact cgtggtactg ggcgatgacc
     5281 tccccggtct ttggggtgct cggcacggct ccatcggggg gcaagccggt caggctcaca
     5341 cgatcggaca cagagggaaa cgcgggcatg aacctgagtg cagggggaac cgtcggtccg
     5401 gaggcggtcg gcgcttcggt gcgagcaaac ggcccacccg tcgcaatgat ccagaccgcg
     5461 gcgacggcca aggcaccacc cacgatcacc ccccaccgtc ggctggcggc gaccgccacg
     5521 gccggtggga cgctttcgtc gtcttgggtc ttgatgggca aactggacat gcggtcgccc
     5581 gacgactccg gttgctggcg aggagcccgg ataacctcgt ggggcgtcgt actctccgga
     5641 acttggtaca ggggtgcctg gcacctggga caggccctcc ccctaccccc gcccgtccag
     5701 tcacagcgca ggcagatccg ttcgcccact tctgtcttgg cctccttgcc aatcacgcgg
     5761 gcacggaccg cagattcttc ggtcggagtc tagaccgccg actcgtcgtg cggcgcgggc
     5821 ccccggtgtg ccccagtgcc cggccctggg acttgcaccc cgtttacacg acgccattgc
     5881 catcggcgcc atga
//
LOCUS       DATBRK010000350         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1222533, whole genome shotgun sequence.
ACCESSION   DATBRK010000350 DATBRK010000000
VERSION     DATBRK010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222533"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VIH70_10465"
     CDS             <1..727
                     /locus_tag="VIH70_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116825.1"
                     /translation="RWVSAVNQTGDQVVLNGGQTIQAFYMSSSGGYTENNENVWGGTP
                     IDYLRGVCDPGDYTSENPNAVWEQTFTPAEVTGHLGLRIGAVTGFTNVDRGVSGRIMS
                     VTVKGANGQARISGEKLRLSLGLPDDRLWINADRSITGDIRAKYDGLGCSPGLPTSRQ
                     VSIAGGVRQKFEEGTIYHSGSAGAHLLRGPVLDRYLIKGGPAGRLGFPTSDVHRLQNG
                     NLRASFEGGVITCDPDDSSCNVT"
     gene            complement(740..1336)
                     /locus_tag="VIH70_10470"
     CDS             complement(740..1336)
                     /locus_tag="VIH70_10470"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEY8116826.1"
                     /translation="MRYAAIAGLIALGWLLFFRTPGPGEVSAAARSAATAAGCGDLGQ
                     PVVPDPSRVHLAPGESFDYPDRPAAAGPHDSSPLPPDPHVYRIPVPETRAVHNLEHAY
                     VIIYYRPPAEDGLAQETIDRLAAIARDEGRVIMAPYPELPDDRAFALLAWNTRWMCPA
                     TISPDQAVTMTTSFIDAFRGTSVAPEAPHGLLGPLFQK"
     gene            complement(1482..2354)
                     /locus_tag="VIH70_10475"
     CDS             complement(1482..2354)
                     /locus_tag="VIH70_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116827.1"
                     /translation="MAKKRRRPQNRSRNRSAASSTRTTERPRPTAPQDVAPDGRRDPE
                     GPEHKAQARRQRPPSHAPAPGPQRSRAEKKELARAEREAARRRIARAERRRQLAWIAG
                     ITVVVAAGVFVFTNRDDQGTSAVALPGELTTEAPWDANAAKSAARATALGLPAEGTTM
                     HEHADVEIFVHGAKEPVPTDIGIDASKGTIQSIHTHDDTGLVHLESSQSREFTLGEFF
                     GVWGVRFTPSCLGAYCNEGNNRLQVFVDGKEVSGSLQDVRLDDQTVIVVTYGTPSELP
                     DPIPSSFDFSSINP"
     gene            complement(2381..>2789)
                     /locus_tag="VIH70_10480"
     CDS             complement(2381..>2789)
                     /locus_tag="VIH70_10480"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEY8116828.1"
                     /translation="SVPPASGPHNAIPYGAGVYSTPPPVDRVIHSLEHGAAIVWYAPD
                     ASGKELDRIRTFYEGQDVGSRVIVAPYDYPDEGAAGTLPAGTRMALVAWHKVQKCENV
                     NLAAAFGFTSSYAAPPFGQQPYEGEAPEAGRPF"
BASE COUNT          387 a          962 c         1014 g          426 t
ORIGIN      
        1 ccgctgggtc tccgcggtga accagacggg cgatcaggtg gtgctgaacg gagggcagac
       61 gatccaggcc ttctacatgt cctcctcggg cgggtacacc gagaacaacg agaacgtgtg
      121 gggcggcacg ccgatcgact acctccgcgg cgtgtgcgac cccggcgact acacctccga
      181 gaacccgaac gccgtctggg agcagacgtt cacccccgcg gaggtgacgg gacatctcgg
      241 gctgcgcatc ggggccgtga ccgggttcac caacgtggat cggggcgtct cgggaaggat
      301 catgtcggtc acggtcaagg gcgcgaacgg ccaggccagg atcagcggcg aaaagttgcg
      361 tctgagcctc ggtctcccgg acgaccggct ctggatcaac gcggaccgga gcatcaccgg
      421 cgacatccgg gccaagtacg acggcctcgg ctgctcgccc gggctcccga cctcgcgcca
      481 ggtctcgatc gccggtggcg tccggcagaa gttcgaggaa gggacgatct accactcggg
      541 ttcggccggc gcgcacctcc tgcggggacc ggtgctggac cgctatctga tcaagggtgg
      601 cccggcgggt cgcctcgggt tcccgacctc cgatgtccac aggctccaga acgggaacct
      661 ccgggcgagc ttcgagggcg gcgtcatcac ctgcgacccc gacgactcga gctgcaacgt
      721 cacctagaac gggtcgccgc tacttctgga acagcgggcc gaggagcccg tggggagcct
      781 ccggcgcgac cgaggtcccg cggaacgcat cgatgaacga cgtcgtcatc gtgaccgcct
      841 ggtccggcga gatcgtcgcg gggcacatcc atctcgtgtt ccaggcgagg agcgcgaacg
      901 cccgatcgtc cgggagctcg gggtaggggg ccatgatcac gcgaccctcg tcccgggcga
      961 tcgccgccag gcggtcgatc gtctcctggg cgagcccgtc ctcggcgggg gggcggtagt
     1021 agatgatcac gtaggcgtgc tcgaggttgt ggaccgcccg cgtctcgggg accgggatcc
     1081 ggtagacgtg cgggtccggc ggcaggggcg acgagtcgtg gggtccggcc gccgccggcc
     1141 ggtcggggta gtcgaacgac tccccgggcg cgaggtggac ccgcgacggg tccggcacga
     1201 cgggctgccc caggtcgccg caccccgccg ccgtggccgc cgaacgcgcc gcggccgaga
     1261 cctcgccggg accgggcgtc cggaagaaga gcagccagcc cagcgcgatg aggcctgcga
     1321 tcgccgcgta ccgcatgatg ccccacgggc ggcgctccgc gggttgaacc gttcggctgc
     1381 gcccggacga gggggtccgc ttcgtcccct tcgggtggga tcccgccgcc ggtcggggac
     1441 cggagggtcg ccgcctgcgc ttcttcgcca acgcggcgcc gctacgggtt gatcgaggag
     1501 aagtcgaacg acgacgggat cgggtcgggg agctcgctcg gggttccgta cgtgaccacg
     1561 atcaccgtct gatcgtccag ccgcacgtcc tgcaggctgc cgctgacctc cttgccgtcc
     1621 acgaacacct ggaggcgatt gttcccctcg ttgcaatagg cccccaggca gctcggcgtg
     1681 aagcggactc cccacacgcc gaagaactcg ccgagcgtga actcgcgcga ttgggacgat
     1741 tcgagatgca ccagccccgt gtcgtcgtgg gtgtggatcg actggatcgt tcccttcgag
     1801 gcatcgatcc cgatgtcggt cgggaccggt tccttggcgc cgtgcacgaa gatctccacg
     1861 tccgcgtgct cgtgcatggt cgtgccttcg gccgggaggc cgagcgcggt cgcacgcgcg
     1921 gccgacttcg cggcgttcgc gtcccagggg gcctcggtcg tgagctcgcc gggcagggcg
     1981 acggcggacg ttccctgatc gtcccggttc gtgaagacga acacgccggc cgccacgacg
     2041 acggtgatcc cggcgatcca cgcgagctgg cgccgacgct cggcgcgcgc gatcctgcgt
     2101 cgggcggcct cgcgttcggc gcgtgcgagc tccttcttct cggcccgaga gcgctgcgga
     2161 ccgggggccg gcgcgtgcga gggcgggcgc tgccgccgtg cctgggcttt gtgttccggc
     2221 ccctccgggt cccgacgccc gtcgggggcg acgtcctggg gagccgtcgg gcgcggccgc
     2281 tccgtcgttc gggtcgacga ggcagccgat cggttcctcg aacggttctg gggcctacgt
     2341 cgcttctttg ccatgtctcg acctcagcgt tctcgctcgg tcagaaaggc ctgcccgcct
     2401 cgggcgcctc gccctcgtac ggctgctgtc cgaacggcgg cgccgcgtac gacgacgtga
     2461 acccgaacgc ggcagccagg ttcacgttct cgcacttctg caccttgtgc caggcgacca
     2521 acgccatgcg ggttccggcg gggagtgttc cggcggctcc ttcgtccgga tagtcgtacg
     2581 gggcgacgat cacgcggctg cccacgtcct ggccctcgta gaacgtgcgg atccggtcga
     2641 gctccttccc cgacgcgtcg ggcgcgtacc agacgatcgc cgcgccgtgc tcgagcgagt
     2701 ggatcacgcg gtcgaccggc ggcggcgtgc tgtagacgcc ggcgccatac gggatcgcgt
     2761 tgtgcggtcc ggacgcaggc ggcacggac
//
LOCUS       DATBRK010000351         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2227214, whole genome shotgun sequence.
ACCESSION   DATBRK010000351 DATBRK010000000
VERSION     DATBRK010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2227214"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VIH70_10485"
     CDS             <1..1348
                     /locus_tag="VIH70_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY8116829.1"
                     /translation="EVLPPSRVLGAVMPEGVRFVGPPPERRPAWRFPLDAPADEDLAA
                     CVACGLCLPHCPTYRVTGEESASPRGRITAMRSVADGLAQPDEAFARFMDLCLACRAC
                     EDVCPSHVPFGRMMERARVQIEPLRTRRARFLRWLGLDLALPRKKALWLAAAMQPLAR
                     VALPRRVRALVPPRSELFRRLPRETPAAGVERGIVSLLSGCVQDRWFREVNRATIRVL
                     TRNGWRVVVPHDQACCGALAAHHGRLDTARALAERNLRAFRDADVVVVNAAGCGAHLQ
                     ELGDLIATDEARAFSTKVRDVMQLLHEEGLVRPPTGRPGFERVAYHDACHALRVQHLR
                     EQPRALLRTIEGIEVLDVPGGDVCCGAAGLYNVLEPEMSSELRRRKADAVASTGADVV
                     ASANPGCTMQIAAGLHELGSPIPVLHPIEILDRGYSAESPEAAPAAPDEPSHIPSA"
     gene            complement(1330..1713)
                     /locus_tag="VIH70_10490"
     CDS             complement(1330..1713)
                     /locus_tag="VIH70_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY8116830.1"
                     /translation="MKDLTVTLEDRPGTLADLGEALGKAGINIEGLTAVSSEGRAIVH
                     VLVEEAAAARTALEGAGIKVEGEAEPLISEVPSGQVDQPGVVGQMARAMADAGVNIQV
                     VYLATKNRAVAVTSDNAKARQALGM"
     gene            complement(1860..2240)
                     /locus_tag="VIH70_10495"
     CDS             complement(1860..2240)
                     /locus_tag="VIH70_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY8116831.1"
                     /translation="MATDLSISLPDEPGGLANAAKALGDAGVNIEGIAGLGGGGHGHV
                     HLLVEDARAARTALEGAGATLEGEREAVIVDVSSEDRPGKLAELAEAVGAAGVNLAAC
                     YVASRSRMVFSSDDAAGLRSALGM"
BASE COUNT          321 a          934 c          848 g          363 t
ORIGIN      
        1 tgaagtcctt ccgccgagcc gcgtgctcgg agcggtgatg cccgaggggg ttcgcttcgt
       61 cggcccgccg cccgagcgcc ggccggcgtg gcggttcccg ctcgacgcgc cggccgacga
      121 ggacctggcc gcctgcgtcg cgtgcgggct gtgcctaccc cactgcccca cgtaccgggt
      181 caccggagag gagtcggcgt ccccccgcgg gcggatcacg gcgatgcgtt cggttgccga
      241 cggactcgcc caaccggacg aggcgttcgc gcgcttcatg gacctctgcc tcgcgtgccg
      301 cgcgtgcgag gacgtctgcc cgtcgcacgt gccgttcggt cggatgatgg agcgcgcgcg
      361 tgtccagatc gaaccgctgc gcacgcgtcg ggccaggttc ctgaggtggc tcgggctcga
      421 ccttgccctc ccacgcaaga aggcgttgtg gctcgccgcc gcgatgcagc cgctggcgcg
      481 cgtcgcgctc ccgagacggg tccgcgcgct cgtcccgccg cggtccgagc tcttccgccg
      541 actcccccgc gagaccccgg ccgccggcgt ggagcggggt atcgtctcgc tgctgagcgg
      601 ctgcgtgcag gatcgatggt tccgcgaggt gaaccgcgcc acgatccgcg tgttgacgcg
      661 caacggctgg cgcgtcgtcg tcccgcacga ccaggcgtgt tgcggggcgc tcgcggcgca
      721 ccacggccgc ctggacacgg cgcgcgccct cgccgagcgc aacctgcgcg cgttccgcga
      781 tgccgacgtc gtcgtggtga acgccgccgg gtgcggagcg cacctgcagg agctgggcga
      841 tctgatcgcg accgacgagg cccgcgcgtt ctcgacgaag gtgcgcgacg tgatgcagct
      901 cctgcacgag gaggggctcg tgcggccgcc gacggggcga cctgggttcg agcgcgtcgc
      961 gtaccacgac gcgtgccacg cgctccgcgt gcagcatcta cgcgagcagc cgcgcgcgct
     1021 gttgcggacg atcgaaggca tcgaggtgct cgacgtgccg ggcggcgacg tctgctgcgg
     1081 ggccgccggc ctctacaacg tcctcgaacc cgagatgtcc tcggagctgc ggcgccgaaa
     1141 ggcggacgcg gtcgcgtcga ccggcgccga cgtcgtcgcg agcgcgaacc ccggttgcac
     1201 gatgcagatc gccgcgggac tccacgagct cgggtcaccg atccccgtgc tccacccgat
     1261 cgagatcctc gaccgggggt actcagccga gtcaccggag gcggccccgg ccgcccccga
     1321 cgagccatct cacatcccga gcgcctgacg cgccttcgcg ttgtcgctcg tgaccgccac
     1381 ggcgcggttc ttggtcgcca ggtacacgac ctggatgttc acgccggcat cggccatggc
     1441 ccgggccatc tggcccacga cgccgggctg gtcgacctgg ccggagggga cctccgagat
     1501 caacggctcg gcctcgccct cgaccttgat gccggcgccc tcgagcgccg tgcgcgccgc
     1561 ggccgcctcc tcgacgagga catggacgat cgctcgtccc tcgctgctga ccgcggtgag
     1621 gccctcgatg ttgatcccgg ccttgcccag cgcctcgccc agatcggcga gcgtcccggg
     1681 ccggtcctcg agggtgactg tgaggtcctt catcggcacc tcctttgttg agacccgccc
     1741 aacccgtgtt gagacccgcc caacccgtgt tgagacccac ccaacccgtg ttgagaccaa
     1801 ccaacccgtc gccttccggt ctacgctcac cgcatcccgg cgtcaatccc cgttcggagc
     1861 tacatcccca gcgccgagcg aagccccgcc gcgtcgtcgg aggagaagac catccgcgag
     1921 cgagaggcca cgtagcaggc cgcgaggttc acgccggcgg cgccgaccgc ctcggcgagc
     1981 tcggccagct tgccgggccg gtcctcgctg gagacgtcga cgatcacggc ctcgcgctcg
     2041 ccctcgagcg ttgcgcccgc tccctcgagg gcggtccgcg cggcacgggc atcctcgacc
     2101 agcagatgga cgtggccgtg cccgccgcct cccagacccg cgatgccctc gatgttcacg
     2161 ccggcatccc cgagcgcctt cgcggcattc gcgagaccgc ccggctcgtc gggcagcgag
     2221 atcgacagat cggtcgccat ccggaccctc ctttcccgtg tgaggaccgg tcctatcccg
     2281 acggaaccca cggcgtcaag ccgttagcat ccccgcgtgc gtgtggccga gcgatggttc
     2341 gagcacgagg aggtcgacga gggcgtcctg cggatccggg aatcgcacgt cgacccgttc
     2401 ctgcaggcga acatgttcct gatccgcggc cgcgaccgcg acgtcctcgt cgacaccggc
     2461 ctgggg
//
LOCUS       DATBRK010000352         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_784519, whole genome shotgun sequence.
ACCESSION   DATBRK010000352 DATBRK010000000
VERSION     DATBRK010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784519"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..478)
                     /locus_tag="VIH70_10500"
     CDS             complement(338..478)
                     /locus_tag="VIH70_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116832.1"
                     /translation="MDSSSGMKRRGGWTWRTWLGIALGVAVLVAVILIAANAGSGSGG
                     IY"
     gene            complement(725..1393)
                     /locus_tag="VIH70_10505"
     CDS             complement(725..1393)
                     /locus_tag="VIH70_10505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8116833.1"
                     /translation="MDRAQGFLFDLDGTLVDSVYQHVLAWRIALDRAGIDLSVWRIHR
                     KVGMSGGLFANALLRETGREVTDQQASELRRLHAEAYAERSTEVRPLPGARELLTYLT
                     LTGVPWAIATSGYRESAGPTLELLELPRDVPVITRDLVERAKPDPDLFLAAADALGMA
                     AENCVVVGDSVWDLLAARRARSLGIGLLSGGYGLEELERAGAYRVYDDPADLLRHLDE
                     IGVR"
     gene            1595..>2290
                     /locus_tag="VIH70_10510"
     CDS             1595..>2290
                     /locus_tag="VIH70_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8116834.1"
                     /translation="MSVLEVTDLAIEVAGTPILEGLTLSLRAGDKTGVVGRNGAGKTS
                     LLDVIAGDEPPLAGRAVVRGRLGYLRQEPRGRETHTHSALSHVLEARGLQELAERLEK
                     FRLAMEERPSDPNVARFARLEERYRAAGGYSGEAEVRRIAAGLGLSDDRLDLPVPALS
                     GGERRRLELTRILFAGSDLLLLDEPTNHLDSDAKHWLMKFLAAYRGALVVVSHDLGLL
                     DGAITRILHLDREG"
BASE COUNT          351 a          822 c          791 g          326 t
ORIGIN      
        1 ggagacgaac gatcagggcg agaaccagga cagcaacgat cagggcggga accaggagaa
       61 caacgatcgg agcgagaacc aggacagcaa cgatcagggc gggaaccaga gcacccggag
      121 accgccgacg acccgaccct ccggcttcgc ctctcggacc cggggtacgt gaccgcgcca
      181 cgaaccgggt ctttcgttag tcgcggcttc gacggtcaca tcgtccggcg cccagtcgac
      241 cgcgagcggc cgttgcggtc ttacatccgg cgtcttcccg gccggggcac cgcttctgcg
      301 gatccggcac cggtcgaatg tgtcgtgaac ctaccgatca gtagatgccg cccgaaccgc
      361 tgcctgcatt ggcggcgatc aggatcacgg ccacgagcac cgcgacaccg agggcgatcc
      421 cgagccacgt cctccaggtc caaccgccgc gtcgcttcat cccgctcgag ctgtccatca
      481 ggaacctcct tcgctcacga acgccgccga tctctacctt ttgccattcg cgcccaaacg
      541 gctccgggtt cccttaccgg ggggtgatca acgtcaccga tcctgcagcg agcccgcgga
      601 atggttcggc gggtggaggc tcggagcgtc agagccggcg ccctggagtc gcgcccgcag
      661 gatctcgcct ccggtcgggt cggagagcgc gacggcagga actcatccgc cgttcccgta
      721 ccagctagcg gacgccgatc tcgtcgaggt gacggaggag gtcggccggg tcgtcgtaga
      781 cgcggtacgc gccggcgcgc tcgagctcct cgaggccgta gccgccggac agcaggccga
      841 tcccgaggga gcgcgctcgc cgggcggcga gcaggtccca gacgctgtcg cccacgacga
      901 cgcagttctc ggcggccatg cccagcgcat cggccgcggc gaggaacagg tcgggatcgg
      961 gcttggcccg ttcgacgagg tcgcgcgtga tgaccgggac gtccctcggg agctcgagca
     1021 gctcgagtgt cggccccgcg gactcgcggt agccgctcgt cgcgatcgcc cacggcacgc
     1081 cggtcaaggt caggtacgtc aggagctctc tggcgcccgg cagcgggcgg acctccgtcg
     1141 agcgctcggc gtacgcctcg gcgtgcaggc gccggagctc gctcgcctgc tggtccgtca
     1201 cctcgcgacc ggtctctcgc agcagggcgt tcgcgaacag tccgccgctc atcccgacct
     1261 tgcggtggat ccgccagacg gacaggtcga tcccggcgcg gtccagggcg atacgccaag
     1321 ccaggacgtg ctggtagacg ctgtcgacga gggtgccgtc gaggtcgaac aggaacccct
     1381 gggcccgatc cacgtcgtca tgctatgggc ggaggtcccc ccgcgcgccg gcgttgtccc
     1441 ctccgccgtg gctcgttcgc tacaatccgc ccgtgagctg ctgaggtcga tctcccgggc
     1501 acgacctgcg ccgactggcg ctccgtgacg cacacccgac caggagacga cccatgttcg
     1561 atcgctcgac ccagccgaca cctcggagcg ctcgatgagc gtgctcgagg tcaccgacct
     1621 ggccatcgag gtcgccggca cgccgatcct cgagggcctg accctgtcgc tgcgcgccgg
     1681 cgacaagacc ggcgtcgtgg gacgcaacgg ggccggcaag acgagcctgc tcgacgtgat
     1741 cgccggggac gagcctccgc tcgccgggcg ggccgtcgtc cgcggccggc tcgggtacct
     1801 tcggcaggag ccacgtggac gcgagaccca tacgcactcc gcgctctcac atgtgcttga
     1861 ggcccgcggg ctccaggagt tggccgagcg gctcgagaag ttccgcctcg cgatggagga
     1921 gcgcccgagc gatccgaacg tcgcgcgctt cgcccggctc gaggagcggt accgagcggc
     1981 cgggggctac tctggcgagg cggaggtgcg ccggatcgcg gccgggctcg gtctgtccga
     2041 cgaccgcctg gacctgccgg tcccggctct ctccggaggg gagcgccgcc gcctcgagct
     2101 cacgaggatc ctgttcgcgg gttcggacct cctgctgctc gacgagccga cgaaccatct
     2161 cgacagcgac gcgaagcact ggctgatgaa gttcctcgcc gcgtaccgcg gcgcgctcgt
     2221 ggtcgtgagc cacgatctcg gcctgctcga cggcgcgatc acccggatcc tgcacctgga
     2281 ccgcgagggc
//
LOCUS       DATBRK010000353         7319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1509200, whole genome shotgun sequence.
ACCESSION   DATBRK010000353 DATBRK010000000
VERSION     DATBRK010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509200"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VIH70_10515"
     CDS             complement(<1..882)
                     /locus_tag="VIH70_10515"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEY8116835.1"
                     /translation="MKVEARTVHDNPSADELRRLTEAMPQSRVTEFGNVNVQTRVTSR
                     SSGSTYVVADRSSGRTITRPAFEEIARLQDEYLRDKDVIVIDGFIGNDPTFRTRARLT
                     IEQANANIAGMQQKLYFPRDDRDEPEMHVIYTPNLEAPGYREDRVIAVDLEHGVTRVL
                     NSDYFGESKKGGLRMWNAKVYDLGGLALHAGLKVVPTAAGEKVFMIIGLSGTGKTTTT
                     FTTQNDSLPVQDDFVALMPGGKPYGTENGCFAKTFSLDPDFEPNIYNAVVKPTTYLEN
                     VYQDEDGKVNFFEERYTQ"
     gene            1039..2046
                     /gene="thiL"
                     /locus_tag="VIH70_10520"
     CDS             1039..2046
                     /gene="thiL"
                     /locus_tag="VIH70_10520"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY8116836.1"
                     /translation="MELTEDELVKAVVRLLSGGEPGVVVGLGDDAAVVEPGDGQLVLT
                     TDLLAEGVHFERGSISARDLGAKAITVNVSDVAAMAASPRYALTAIAAPSDVDASWVV
                     ELVGGMRDACAEYALALVGGDTNRADLVVVSVAVVGEVAPGRAVLRSGARVGDRIVVT
                     GSLGAAAGGLALSRAATGRAAEALSQPWGRALIEALERPVARVGEAQVLARLGATAMI
                     DLSDGLSTDLSRLCEASGVGARVFLGSVPVAETLSRGAEMLDVDASALALSGGEDYEL
                     LATLPGDAVEVARGELRAAFGVDLSEIGEIVEGSAVVAVDAEDRTAPLEPSGWDHFAR
                     G"
     gene            2056..2871
                     /gene="thiD"
                     /locus_tag="VIH70_10525"
     CDS             2056..2871
                     /gene="thiD"
                     /locus_tag="VIH70_10525"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY8116837.1"
                     /translation="MDDQVRPPRPRALSIAGSDSGGGAGIQADLKTFSALGVFGMTAI
                     TAVTIQNTRGVSGYETLPPQVVADQIRAVVTDIGVDAAKTGMLASAQIVEAVAEAVAE
                     TAISNLVVDPVSVSKHGHRLLDEDAVGVLRERVLPLATLVTPNLPEAAVLAGFEVTDR
                     DLMQDAAETIQAMGPEAVLVKGGHLPGATGADDLFFDGDRMAWIAGERIETPNTHGTG
                     CTLSSAIAAHLARGAELLEAVREGKAFVTEAIRAALPLGGGIGPVDQLWGIRT"
     gene            complement(2945..3136)
                     /gene="rpmB"
                     /locus_tag="VIH70_10530"
     CDS             complement(2945..3136)
                     /gene="rpmB"
                     /locus_tag="VIH70_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY8116838.1"
                     /translation="MAAVCDICGKKPFFGMQVSHSHRRSPKRWDPNVQRVRALVNGTP
                     RRLHVCTSCLKAGKVQRAI"
     gene            3375..6821
                     /locus_tag="VIH70_10535"
     CDS             3375..6821
                     /locus_tag="VIH70_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8116839.1"
                     /translation="MTESLLPGEERKRVTVLFADLAGSTGLGEQLDPEHLNEVTDSFF
                     QKMRAELEAEGGTVEKFIGDAVMAVFGVPAVHEDDATRALRAALRMRRALDRLNRSLE
                     ESHGIRLAMRTGVNTGEVVARTVPRPGEGLVTGDVVNVAARLEQRAESGQILVGERTA
                     RAARGLRLETIGPLMLKGKEEPVVAYEVLDDEVTAPGSGRRGEPGRYRAPLVGRREEL
                     GVLKSVFERSVIERRSHLVTVFGDAGVGKSRLVEEFGAWAQALAEPALAIGGRCLPYG
                     EGVTYWPLAQILKGWAGVLDSDEPETALDRLRHAVGKLLADVPDVAAEPEELTAALAF
                     TVGLQDPARPVEEDPREARAATHEAWRIFLSSLARPRPVVVVIEDIHWADPALLELLQ
                     ELAEHSEGPLMFVCPARPELTARRPDWGGGRWNYSSLLLEPLSKGDAGDLLDLLLVDA
                     GEVPGSLRARILERAGGNPFFLEEIVLHLMEEHPGPEIEDVRIPDTVQGVLAARMDLL
                     PASEKRVLQTASVVGRTFWNGAVASILDGEDRPHDLDDILWRLEDRGLVHARMGSTIV
                     GEREFSFRHILTQEVAYESLPRRERATAHARVAGWIERQTAEREREFAELLAHHYEEA
                     YRGVLETSRPGAADRERLRERAFHHAMLAANEARRKLALEQAERFAATALSVAEGTRE
                     HADSLEALGMTYFHDYRGDIAWDHLKEAIDLRAGSSEDLGEGSSRSLAHLCAAALEIV
                     TRARGTMRHRVTREEGERYLAIGLASAGEGDDEDRVRLLVAASFFPFALKEVRLSDED
                     LEPARRAGEEAAAMADRLGRVDLRSAALDGISSLHQSIGRYGPVGEAIARRLELVPKL
                     TDPYEIGDIYAMAAWWALSTGRYREAAGFADRGVREAQAGSPAQGLYCLDFRTAANFR
                     LGDWDETLADVAVAEELLGDRRETPPGFAPQYLVIAAFIHDARGERAMADRYVQLVGW
                     LEGVEDRPDTVVALWRSRLLARQGRSQEARALLERPGIADDQRGRDEVLEAWCELVAE
                     EGAWGDAPAIVEQARSHATWAGVPPLELYAGRLQGRLNAATGDREGAADLLRIAADGF
                     AGLEAEWEAAVTRLELATVEASLGRATEARDRYEDVIPVFERLRSVRELDRANGLVAR
                     LT"
     gene            complement(6828..>7319)
                     /locus_tag="VIH70_10540"
     CDS             complement(6828..>7319)
                     /locus_tag="VIH70_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY8116840.1"
                     /translation="GLIDQLRNGGMPPGVRYVASVTGSSQVIAVLSYDELIELPAIIR
                     SLSEGDSTALPLRTSQIRKSEYREKTAFIRIQTKVGDPETLLAPIREAIGSDEADVVF
                     GDFDILACVVGDSIEDLKTRVVGSLRTIEGISRTETLIVIDYVSLSENAPDGHRGTTK
                     SMA"
BASE COUNT         1074 a         2425 c         2650 g         1170 t
ORIGIN      
        1 ctgcgtgtag cgctcctcga agaagttcac cttgccgtct tcgtcctggt agacgttctc
       61 gaggtaggtc gtcggcttga cgaccgcgtt gtagatgttg ggctcgaagt cgggatcgag
      121 gctgaacgtc ttcgcgaagc acccgttctc ggtcccgtag ggcttcccgc cgggcatcag
      181 cgcgacgaaa tcgtcctgca ccggcaacga gtcgttctgc gtcgtgaacg tcgtcgtggt
      241 cttcccggtg cccgagagcc cgatgatcat gaacaccttc tcgccggcgg ccgtcgggac
      301 gaccttcaaa ccggcgtgga gggccaggcc gccgaggtcg tagaccttcg cgttccacat
      361 ccgcaggccg cccttcttcg actcgccgaa gtagtcggag ttcaggacgc gggtcacgcc
      421 gtgctcgaga tcgacggcga tcacgcggtc ctcccggtag cccggcgcct cgaggttcgg
      481 cgtgtagatc acgtgcatct ccggctcgtc gcgatcgtcg cgcgggaagt agagcttctg
      541 ctgcatcccg gcgatgttcg cgttcgcctg ctcgatcgtg aggcgcgccc gcgtccggaa
      601 tgtcggatcg ttgccgatga agccgtcgat cacgatgacg tccttgtcgc ggaggtactc
      661 gtcctggagc cgagcgatct cctcgaacgc cggccgggtt atcgtccggc cgctcgaacg
      721 atcggcgacc acgtacgtgc tgccggagct cctcgacgtg acccgggtct ggacgttcac
      781 gttgccgaac tccgtgaccc gggactgcgg catcgcctcc gtgaggcggc ggagctcatc
      841 cgcggacggg ttgtcgtgga ccgtgcgcgc ctcgaccttc aacggcgttc tccttcgtcg
      901 ggaaggctcg cgagctcgga ccgctcggag cggcccttat cctacccaag gcgaggcggc
      961 cgaccgctgc cgcgacgccg ggcccgcggg ggccgaagga acgggcccaa ggaacggccc
     1021 aaggagccag atggacccat ggaactgacc gaggacgagc tggtcaaggc cgtggtgcgc
     1081 ctgctctccg ggggcgagcc gggcgtcgtg gtgggtcttg gcgacgacgc cgcggtcgtc
     1141 gaacccggcg acggccagct cgtgctcacg acggacctcc tcgccgaggg cgtgcacttc
     1201 gagcgcggat cgatctcggc gcgggacctc ggcgccaagg cgatcaccgt gaacgtctcg
     1261 gacgtggcgg cgatggcggc gagcccccgg tacgcgctca cggcgatcgc ggctccgagc
     1321 gacgtcgacg cgtcctgggt ggtggaactg gtcggcggga tgcgtgacgc gtgcgcggag
     1381 tacgcgctcg cgctcgtcgg cggcgacacg aaccgggcgg acctcgtcgt cgtatcggtg
     1441 gccgtggttg gggaggtcgc gccgggccgc gcggtgctgc gttcgggcgc ccgcgtgggg
     1501 gaccggatcg tggtgaccgg ctcgctcggc gcggctgcgg gcggcctcgc gctctcgcgg
     1561 gcggcgaccg gccgagcggc cgaggccctg tcgcagccgt ggggtcgcgc gctgatcgag
     1621 gcgctcgagc gacccgtcgc gcgcgtcgga gaggcgcagg tgctcgcgcg cctcggcgcg
     1681 accgcgatga tcgatctgtc cgacggcctg tcgaccgacc tgtcgcgact gtgcgaggcg
     1741 agcggcgtcg gcgcgcgcgt cttcctcggc tccgtccccg tcgcagaaac cctgtcgagg
     1801 ggcgcggaga tgctcgacgt cgacgcctcg gcgctcgcgc tctccggagg cgaggactac
     1861 gagctgctcg cgacgctccc cggggacgcc gtcgaggtcg cccgcggaga gctgcgagcg
     1921 gcgttcggcg tcgacctgtc cgagatcggc gagatcgtcg agggatcggc cgtcgtggcg
     1981 gtggacgccg aggaccggac ggcgccactc gaaccctcgg gctgggatca cttcgcccgg
     2041 gggtagcctg gtctcgtgga cgatcaggtt cgaccgccac gaccaagagc gctctcgatc
     2101 gccggttcgg actcgggcgg cggcgccggg atccaggccg acctcaagac gttcagcgcg
     2161 ctcggcgtgt tcgggatgac cgcgatcacc gccgtcacga tccagaacac gcgaggcgtc
     2221 agcgggtacg aaacgctccc accgcaggtc gtcgccgacc agatccgtgc ggtcgtcacg
     2281 gacatcgggg tcgatgcggc caagaccggg atgctggcct ccgcgcagat cgtcgaggcg
     2341 gtcgccgagg ccgtcgccga gacggcgatc tcgaacctcg tcgtggaccc ggtctcggtc
     2401 tccaagcacg gccatcgcct cctcgacgag gatgcggtcg gggtcctgcg cgagcgcgtc
     2461 ctgccgctcg ccacgctggt cacgcccaac ctgcccgagg ccgccgtcct ggccgggttc
     2521 gaggtgaccg accgcgacct gatgcaggac gccgccgaaa cgatccaggc gatggggccg
     2581 gaagcggtcc tggtcaaggg cggccacctc ccgggcgcga ccggcgccga cgacctgttc
     2641 ttcgacggcg accggatggc gtggatcgcg ggcgagcgga tcgagacgcc gaacacccac
     2701 ggcacggggt gcaccctgtc gtcggcgatc gccgcccacc tcgcgagggg cgccgagctc
     2761 ctcgaggccg ttcgggaggg caaggcgttc gtcaccgagg cgatccgggc agcgctcccg
     2821 ttgggcggcg ggatcgggcc cgtcgatcag ctctggggga tccggacgta gaacgggctc
     2881 ggacgttgag cgcgcgcggt cggtcgaagc acccgggttc ggcgcgagat cgggtgaccg
     2941 agagttagat ggcgcgctgg accttgccgg ccttcaggca gctcgtgcaa acgtgcaggc
     3001 gcctgggcgt cccgttcacg agtgcgcgga cgcgctgcac gttggggtcc caccgcttgg
     3061 gggagcggcg gtgcgagtgg gagacctgca tgccgaagaa cggcttcttc ccgcagatgt
     3121 cgcagacggc agccatggcc cagcgagggt agcagacgcg catttcgctc tggcgcgacg
     3181 gctgctcagc acgcctatga aggaatagga gctttcccta gacttgcctg gtgaggacgt
     3241 gctcgacctg cgacgcagag aatccagata cggctcggtt ctgcgtgcag tgcggggcgc
     3301 agcttgcggt ctgtgtggca tgcggcgccg gcctccctcc cgacgtgcgg ttctgtccgg
     3361 catgcgggac acaggtgacg gagtcgctct tgcctggcga ggagcgcaag cgcgtcacgg
     3421 tcctgttcgc tgaccttgct ggctcgacag gcctcggcga gcagctcgac ccggagcatc
     3481 tgaacgaagt cacggactcc ttcttccaga agatgcgggc cgagctcgaa gctgaaggtg
     3541 gcacggtgga gaagttcatc ggcgacgcgg tgatggcggt cttcggggtt cctgcggtgc
     3601 acgaggacga cgccacacga gcgctccgcg cggcgctgcg catgcggcgc gcgctcgacc
     3661 gactgaatcg ctcgctggag gaatcccacg gcatccgctt ggcgatgcga accggcgtca
     3721 acacgggtga ggtggtagcc aggaccgtac cgcggcctgg cgaggggctc gtcacgggtg
     3781 acgtcgtgaa cgtggccgcg cgcctggagc agcgggcgga atcgggtcag atcctcgtcg
     3841 gcgagcgcac cgcacgggcg gcacgcggct tgcggctcga aacgatcggt cccctcatgc
     3901 tgaagggcaa ggaagaaccg gtcgtcgcgt acgaggttct cgacgatgag gtgacggcac
     3961 cgggctccgg acgacgaggc gagcccggcc gataccgggc acccctcgtc ggtcgccgcg
     4021 aggagctggg cgtcctgaag agcgtcttcg aacgctccgt gatcgagcgc cgttcgcacc
     4081 tcgtgaccgt gttcggggat gcgggcgtcg gcaagtcccg gctcgtcgag gagttcggag
     4141 cgtgggcgca agcgcttgcc gagccggcgc tcgcgatcgg tggccgctgc ctaccctacg
     4201 gtgagggcgt cacctactgg ccgctggccc agatcttgaa gggctgggcc ggcgtcctcg
     4261 acagcgacga gcccgagacc gccctcgacc ggctccgtca tgcggtgggg aagctgttgg
     4321 ctgacgttcc cgatgtggct gccgaaccgg aggaattgac ggcggccctc gcgttcaccg
     4381 tcgggctcca agacccggcc aggccggtcg aggaggatcc gcgggaggcc cgagcggcca
     4441 cgcacgaggc ctggcggatc ttcctgtcct cgctggcgcg tccgcgcccg gtcgtggtgg
     4501 tgatcgagga catccactgg gcggatcccg cgctgctcga gctcttgcag gagctagccg
     4561 aacacagcga agggcccttg atgttcgtct gtcccgcacg gcccgagctg accgcccgcc
     4621 gccccgattg gggcggcggg cggtggaact attcgagcct cctcctggag ccgctctcca
     4681 agggcgacgc gggtgatctt ctggatctcc tcctcgtcga cgccggggaa gtgcccggct
     4741 ccttgcgggc tcgcatcctc gagcgcgccg gcgggaatcc ctttttcctg gaggagatcg
     4801 tgctccatct catggaggag catccggggc ccgagatcga ggacgtccgg atcccggaca
     4861 ccgtccaagg ggtcctcgcg gccaggatgg acctcctgcc cgcgtccgag aagcgcgtgc
     4921 tgcagacggc ctcggtcgtg ggccggacct tctggaacgg cgccgtggcc tccatcctcg
     4981 atggggagga ccgcccgcac gatctagacg acatcttgtg gcgccttgag gaccgtgggt
     5041 tggtccacgc gaggatgggg tcgacgatcg tgggcgaacg cgagttctcg ttccgccaca
     5101 tcctgacgca ggaggtggcc tacgagagtc tcccacgacg cgagcgagcg accgcccacg
     5161 ctcgggtcgc gggctggatc gagcgacaga ccgccgagcg cgagcgcgag ttcgccgaac
     5221 tgctcgcgca tcattacgag gaggcctacc gtggggtcct cgaaacgtcc cgcccggggg
     5281 cggccgacag ggagcggctg agggagcgcg cgttccacca cgcgatgctc gccgcgaacg
     5341 aggcccgaag gaaactcgcg ctcgagcagg cggagcggtt cgcggccacc gctttgtcgg
     5401 ttgccgaggg aacgcgcgag cacgcggact cgctcgaggc gctcggcatg acgtacttcc
     5461 acgactatcg cggcgacatc gcctgggacc atctcaagga ggccatcgat cttcgagcgg
     5521 gatcctccga agacctcggc gagggctcgt ctcgctcgct cgctcacctg tgcgctgccg
     5581 cgctcgagat cgtcacgagg gcccggggga cgatgcggca ccgcgtcacc cgggaggaag
     5641 gcgagcggta cctggcgatc ggtctcgcat ctgctggcga gggtgacgat gaagaccgcg
     5701 tacgcctcct ggtggcagcg tcgttcttcc ctttcgcgct caaagaggtc aggctgagcg
     5761 acgaagacct tgaaccggca cgccgggcgg gcgaggaggc cgcggcgatg gccgaccgcc
     5821 tggggcgcgt cgatctgcgc tctgctgccc tcgacgggat ctcctcgctc caccaatcga
     5881 tcgggcgcta cgggccggtg ggcgaggcga tcgctcgccg tctcgagctc gttcccaagc
     5941 tcaccgatcc gtacgagatc ggcgacatct atgcgatggc agcctggtgg gccctgagca
     6001 ccggtcgcta ccgcgaggcg gccgggttcg cggaccgagg cgtccgtgag gcgcaggccg
     6061 gctcaccggc gcaggggctg tactgcctcg acttccggac ggcggcgaac ttccgcttgg
     6121 gcgactggga cgagacgctc gccgacgtcg ccgtcgccga ggagctcctc ggcgaccggc
     6181 gagagacgcc gccggggttc gcgccccagt acctggtgat cgccgccttc atccacgacg
     6241 cgcgcggaga acgcgccatg gccgatcggt acgtgcagct ggtcggctgg ttggagggtg
     6301 tggaggatcg tcccgatacc gtcgtcgccc tttggcgatc gcgtctgctg gcccgccagg
     6361 ggcggtccca ggaagctcgc gctctgttgg aacgaccggg gatcgccgac gaccagcggg
     6421 gcagggacga ggtcctcgag gcctggtgcg agctggtcgc cgaggaagga gcatggggag
     6481 atgcgccggc gatcgtcgag caggccagga gccacgccac gtgggcgggc gtccccccgc
     6541 tcgagctcta cgcggggcgc ctgcaggggc gactgaatgc tgccaccgga gaccgtgagg
     6601 gagccgcgga cctgcttcgg atcgcggccg acgggttcgc cggcctcgag gcggagtggg
     6661 aggcggcggt cacgaggctg gagctggcga ccgtcgaggc ttcgctgggg agagcgacgg
     6721 aggcgaggga ccggtacgag gacgtcatcc ccgtcttcga gcgtcttcgg tctgttcgcg
     6781 agctcgatcg ggcgaacggt ctggttgctc gattgacgtg atgcccgtca cgccatcgac
     6841 ttcgtggtcc cccggtgacc gtcgggtgcg ttctcgctga gtgagacgta gtcgatcaca
     6901 atgagcgtct ccgttcgact gatcccctcg atcgtccgca acgagcccac gaccctggtc
     6961 ttcaagtctt cgatcgagtc cccaaccaca cacgcgagga tgtcgaaatc accaaagacc
     7021 acatccgcct cgtctgatcc gatcgcctcg cggatcggag cgagcagggt ttccgggtca
     7081 cccaccttcg tctgaatcct gatgaacgcc gtcttctccc ggtactcgct cttcctgatc
     7141 tgactcgtcc tcaatgggag cgcagtggaa tcgccttcgg agagtgacct gatgatcgcc
     7201 ggcaattcga tgagctcgtc gtagctcagc accgcgatga cctgcgatga ccccgtcacc
     7261 gacgcgacgt acctgacgcc tgggggcatc ccgccattcc tcagctgatc gatcagccc
//
LOCUS       DATBRK010000354         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_66946, whole genome shotgun sequence.
ACCESSION   DATBRK010000354 DATBRK010000000
VERSION     DATBRK010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66946"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VIH70_10545"
     CDS             complement(<1..458)
                     /locus_tag="VIH70_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEY8116841.1"
                     /translation="MSGQRTGKATAELFEQVILRRLGAKDADVLIGPRHGVDVGVVRV
                     ANGVAMALTADPVFIVPAYGWERAAWFAVHILASDAATSGLPLRWMAVDLNLPPEITD
                     EELASLWDAFHRACEDIGLAVVTGHTARYDGCAWPMVGGAVCIAAGPEDR"
     gene            complement(455..1087)
                     /gene="thiE"
                     /locus_tag="VIH70_10550"
     CDS             complement(455..1087)
                     /gene="thiE"
                     /locus_tag="VIH70_10550"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY8116842.1"
                     /translation="MDPRSLRVYVLTSSGLVPGRSHRDVAEAALAGGATAVQLRAPEL
                     IDARLEALAGELVERCHATGTLFVVNDRVAVAASVGAGAHVGQADDPGSAREILDPTA
                     VLGVSVSTTGEARAAEIAGADYIGVTVWASATKPEAEPRGLDGLRTIARATGLPVVGI
                     GGVHAGNAADVLDAGAAGICVVSAVGAAPDPVRATAELVEAVGRWEEQTG"
     regulatory      complement(1083..1197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1220..1921)
                     /locus_tag="VIH70_10555"
     CDS             complement(1220..1921)
                     /locus_tag="VIH70_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116843.1"
                     /translation="MRDPDGYPAEFERIEAAVDAGQTDLRALGFWRLLARVKADPVLA
                     HHWAEPAGRIDRKAFEAHTRFRLPVWLGNAVLVIGSLVGAAAVAFAMTTNSAGLAGWA
                     LVFAALDWSATLHVPAHWLVGRAIGMRFLAYYVRDLIPPIPGLKIDYATYLRVSPEAR
                     AWMHASGAIASKIGPFLALAFWPASGAAGWAAWAILGYGLLIIGTDVFISTRKSDWKR
                     FRREMRIARIQAANR"
     gene            2053..>3206
                     /locus_tag="VIH70_10560"
     CDS             2053..>3206
                     /locus_tag="VIH70_10560"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8116844.1"
                     /translation="MHLAFALNTLDGRYHVADRIAAGGMGEVYRARDAVLERPVAIKV
                     LHRNLAGDAGFIERFRREARAAANLNHQNIVAVHDWGSVDGIYYMVMEYVAGLSVREI
                     LHAEGLLAPAQAADVLEQTLAALQHAHRQGIVHRDVKPENLMVTRDGVVKVADFGLAR
                     AFADAQITEAGNVIGTVQYLAPEQLQGEPADPRTDLYALGVVAFELLTGRLPFTGETP
                     MAIAYQHIHGPMPRPSSANPAIPASLDGWVASVTEKQRELRPESAAEARRDLQAEAGS
                     LPAAPPIASLVPEVAVIPDGGVVEAGAARSGHPAETVTIPGRDLGRSKRKRRKGRLAF
                     GIVLALLAIGSAAWASWTYLIPHEVDVPKVVGLTVEHAQVQLDEAGLVVR"
BASE COUNT          463 a         1215 c         1115 g          413 t
ORIGIN      
        1 aaccgatcct cggggcccgc ggcgatgcag acggcgccgc cgaccatggg ccaggcacag
       61 ccgtcgtacc gcgccgtgtg acccgtcacg acggccaacc cgatgtcctc gcacgcgcga
      121 tggaacgcat cccagagcga ggcgagctcc tcgtcggtga tctcgggcgg caggttcagg
      181 tcgacggcca tccaccggag cggcaggccg ctcgtggcgg cgtcggacgc gaggatgtgg
      241 accgcgaacc acgccgcccg ctcccacccg taggccggga cgatgaagac cgggtcggcc
      301 gtgagcgcca tcgcgacgcc gttcgccacg cggacgacgc cgacgtccac gccgtgccgg
      361 ggcccgatca gcacgtcggc gtccttcgcg cccagccgcc gaaggatcac ttgctcgaac
      421 agctccgccg tcgccttccc cgtgcgctgc ccgctcatcc cgtctgttcc tcccaacggc
      481 ccacggcctc caccagttcc gccgtggcgc ggacggggtc cggcgccgcg ccgaccgccg
      541 acaccacgca gatccccgcc gccccggcgt cgagcacgtc ggcggcgttc ccggcatgga
      601 cgcccccgat ccccaccacc ggcagccccg tcgcccgggc gatcgtccgc agaccgtcca
      661 gaccccgcgg ctcggcctcg ggcttggtcg cactcgccca gacggtgacg ccgatgtagt
      721 cggcgcccgc gatctcggcc gcgcgcgcct cgccggtcgt cgagacgctg accccgagca
      781 ccgccgtcgg gtcgaggatc tcccgcgccg atcccgggtc gtcggcctga ccgacgtggg
      841 ccccggcgcc gacgctcgcg gccaccgcca cgcggtcgtt cacgacgaac aacgtgccgg
      901 tggcgtggca ccgctcgacg agctcgcccg cgagcgcttc cagacgcgca tcgatgagct
      961 cgggggcgcg gagctgcacg gcggtggcgc ccccggccaa cgcggcctcg gccacgtcgc
     1021 gatggctccg accggggacg aggccgctcg acgtcagcac gtagacgcgg agcgaacgtg
     1081 gatccaccgt agaccgcgcc tccctccgcc ggcattaccc ggatcaggtt ggttcgggtc
     1141 gccgccgcga tctggcggcc tctcagcccg gttgcccgag ctccccggtt ctcagctcag
     1201 gatagccgag gccgcgggtt cagcggttcg cggcctggat ccgggcgata cgcatctcgc
     1261 gccggaaccg cttccagtcg ctcttgcgcg tcgagatgaa gacgtccgtc ccgatgatga
     1321 ggaggccgta cccaaggatc gcccacgcag cccatcccgc ggccccgctc gcgggccaga
     1381 acgcgagcgc gaggaagggg ccgatcttgc tcgcgatcgc ccccgaggcg tgcatccagg
     1441 ccctcgcctc cggtgagacc cgcaggtacg tggcgtagtc gatcttcaga ccggggatgg
     1501 gcgggatcag gtcgcggacg tagtaagcga ggaaccgcat cccgatcgcc cggccgacca
     1561 gccagtgcgc cggcacgtgg agcgtcgcgg accagtcgag ggccgcgaag accaacgccc
     1621 aaccggcgag ccccgccgag ttcgtcgtca tcgcgaacgc caccgccgcg gcgccgacga
     1681 gcgagccgat cacgagcacc gcgttcccga gccagaccgg caggcggaag cgcgtatgcg
     1741 cctcgaacgc cttccggtcg atccgacccg ccggttcggc ccagtggtgg gcgaggaccg
     1801 ggtccgcctt caccctggcg agcaggcgcc agaagccgag cgctcgaagg tcggtctggc
     1861 cggcgtcgac ggcggcctcg atgcgctcga attcggccgg gtagccgtcc gggtccctca
     1921 cccctgctcc acggttgacg ctcgtccccg cgctaggatg ttcgctcgga cacgcccccg
     1981 ggcccaggta gtccgcccgc aacgaccgag cagcgacggg aggcgaccat ctcgaagagc
     2041 accatccccg acgtgcatct cgcgttcgcg ctgaacacgc tcgacggccg gtaccacgtc
     2101 gccgatcgta tcgccgccgg cggcatgggc gaggtgtacc gcgcccgcga cgcggtgctc
     2161 gagcgtccgg tggcgatcaa ggtcctgcac cggaacctcg cgggcgacgc gggcttcatc
     2221 gaacgcttcc ggcgggaggc acgcgccgcc gcgaacctca accaccagaa catcgtggcc
     2281 gtccacgact ggggaagcgt cgacgggatc tactacatgg tgatggagta cgtcgccggg
     2341 ctctcggtcc gcgagatcct gcacgccgag ggcctcctcg ccccggcgca ggcggccgac
     2401 gtgctcgagc agacgctcgc ggcgctccag cacgcgcacc gccagggcat cgtgcaccga
     2461 gacgtgaagc cggagaacct gatggtgacg cgcgacggcg tggtcaaagt cgcggacttc
     2521 gggctcgcgc gggcgttcgc cgatgcgcag atcaccgagg ccggcaacgt gatcgggacc
     2581 gtgcagtacc tggcgcccga gcagctccag ggcgagcccg ccgacccgcg caccgacctg
     2641 tacgcgctgg gggtcgtcgc gttcgagctg ctgacgggcc ggctcccgtt caccggcgag
     2701 accccgatgg cgatcgcgta ccagcacatc cacgggccga tgccgaggcc gtcatcggcg
     2761 aatccggcga tcccggcgtc gctcgacggc tgggtggcct cggtgaccga gaagcagcgc
     2821 gagctccgtc ccgagtcggc ggccgaggct cggcgcgatc tgcaggccga ggcgggttcg
     2881 ctgccggcgg cccccccgat cgcgtcgctc gtgcccgagg tggcggtgat ccccgacggc
     2941 ggcgtcgtag aggccggcgc ggctcgctcg ggtcacccgg ccgagaccgt cacgatcccc
     3001 ggacgcgacc tcgggcgttc gaagcggaag cgccgcaagg gccggttggc attcgggatc
     3061 gtgctcgcgc tcctcgcgat cggcagcgcg gcatgggcct cgtggacgta cctgatcccg
     3121 cacgaggtcg acgtccccaa ggtcgtcggc ctcaccgtcg agcacgccca ggtgcagctc
     3181 gacgaggcgg ggctcgtcgt tcggat
//
LOCUS       DATBRK010000355        12234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1941479, whole genome shotgun sequence.
ACCESSION   DATBRK010000355 DATBRK010000000
VERSION     DATBRK010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12234
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941479"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VIH70_10565"
     CDS             complement(<1..288)
                     /locus_tag="VIH70_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY8116845.1"
                     /translation="MAPLLLLNAEDVRNLIDLPVLFDALEVALRELSAGNASVPPRTV
                     ARTPNGFLGSMPGYLPGTLEAKLVAVFPGNHDRGLPSHQALIALFDEETGAP"
     gene            complement(348..1520)
                     /locus_tag="VIH70_10570"
     CDS             complement(348..1520)
                     /locus_tag="VIH70_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8116846.1"
                     /translation="MRGKRLRFLAAGVLALSLVAAACGNDDEGGGGSTAGGEAGGGAD
                     CASTDQVTLQLQWFIQAQFAGYYAAQDQGFYADQCLEVTIVEGGVDIVPQQQLADGAV
                     DFALAWVPKALATREAGANIVDIAQVFQRSGTLQVSFAEAGITTPADFAGKTIGNWGF
                     GNEYEIFAALAQANLDPATDVTLVQQDFDMSGLLSGDIDAAEAMIYNEYAQVLETVNP
                     DTGELYQPSDLNVISYEEVGVGMLQDAIWADGDRLESDDAYRDIAVRFVTASLQGWAY
                     CRDNVQECTDLVVAQGSKLGASHQLWQMNEINQLIWPAPDGVGVIDQEAWDRTAEIAQ
                     NTPNLEGTTVLTAPPDAGAYTNDIVNEALANLQDLGVDTTGDSYQPIEVTLNEGGA"
     gene            complement(1578..2369)
                     /locus_tag="VIH70_10575"
     CDS             complement(1578..2369)
                     /locus_tag="VIH70_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8116847.1"
                     /translation="MTRSRRHPFGLIWPPVVFGVAFLAIWETAVNVFDFQPYFLPAPS
                     AIWDAFRSNTALIWEATKVSGSNALIGLVLGTAIGVGMAFLLARFRLADDLMSPLAVA
                     LNAIPIFVLVAVFNNMYPVTSEVPRRLMATLVVYFIVLINVARGLREVGSVYLDLMRS
                     YAASDVTVLRKVRVPHAVPYLFTALRIAAPASVITAFVSEYFGGLQNGLGSRIVSNIA
                     LSNNAEAWAYVLGACLLGLAFFLTAAALERVATPGGVGARERDRA"
     gene            complement(2366..3208)
                     /locus_tag="VIH70_10580"
     CDS             complement(2366..3208)
                     /locus_tag="VIH70_10580"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8116848.1"
                     /translation="MNSAPAGGDGTSAVEVRGLTKVFNAGRPDAVEALADIDLIIAPG
                     EFVSLIGPSGCGKSTQLRLIANLTEPTTGEVLVNGKPARTARLDQDYGMAFQQSGLFE
                     WRSVAKNVELPLELKGWDKARRRARALEMLDLVKLPEFAEHFPWQLSGGMQQRVAIAR
                     ALAAHPPLLLMDEPFGALDEMTREHMQTELLRICGETSTTVVFVTHSIPEAVFLSTRV
                     VVMSPRPGRITDVVDVELGPRRSVDTRETEAFFAKVTEVREALRGIQPAGIEPDIEQM
                     AQEP"
     gene            complement(3205..4071)
                     /locus_tag="VIH70_10585"
     CDS             complement(3205..4071)
                     /locus_tag="VIH70_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8116849.1"
                     /translation="MTRFRRVWTFALALILVAVVWELYKVIGPEDGGTVFGWTILPRA
                     NDTAMPHVWEMVSRYGQPENRASDTPILVVVLRGAWFTFRIALAGLALGTAVGLGLAV
                     LMARYKVVERGLLPYLVLSQTVPLIALAPLVVSWGGRLHVGAFEWPRWLSAAVLGAFL
                     AFFPVAVGTLKGLTSTPPASLELMDSYAASWRQTLFKLRFPAALPYIVPALKLAATAS
                     VVGVVVAEISTGLAGGIGRLIIEYAREATSDPAKVFTAVFGAAALGLVMAGIVALVDL
                     VGMRNRPREAME"
     gene            complement(4068..5084)
                     /locus_tag="VIH70_10590"
     CDS             complement(4068..5084)
                     /locus_tag="VIH70_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEY8116850.1"
                     /translation="MDVGVVLQTTPPSARVIDLGTKAETYGFTHVWTFDSHILWEEPF
                     PIYTQILANTRKVTVGSMVTNPATRDWTVTASVFATLNEMFGNRTVIGIGRGDSAVRV
                     INGKPTTLATLRESVQVIRELVNGRTAEYRGSRIRFPWASRSRAEVWVAGYGPKALKL
                     IGEVGDGFILQLADPHITEWTIGAVRTAAEEAGRDPDDVMICVAAPAYVTDDVAHARD
                     QCRWFGGMVGNHVADIVARYGETSAVPQELTDYIKGREGYDYNEHGQAGNVHTEFVPD
                     EIIDRFCVLGPVEEHVRRLEELKALGVHQFAVYLQHDAKDETLQAYGEKVIPAIADTV
                     RAKS"
     gene            complement(5122..5964)
                     /locus_tag="VIH70_10595"
     CDS             complement(5122..5964)
                     /locus_tag="VIH70_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY8116851.1"
                     /translation="MSRTVKAALLQTDWTGDQESMTDKHEEAAREASAQGAEVICFQE
                     LFYGPYFCQVQDTEYYSYTEPIPDGPTTKRFQSVAKELGMVMVLPMYEFVQAGQYFNT
                     AAVVDADGTYLGKYRKQHIPQVKGFWEKFYFTPGTEGYPVFETAVGKVGVYICYDRHF
                     PEGWRILGLHGAEIVFNPSATHRGLSEYIWRLEQPAAAVANMYYVGAINRVGIEPLGE
                     NDFYGQSYFVDPEGQFVGEVGDAYKPELIVRELDLDKIREVRDRWAFYRDRRPDAYDP
                     IVAR"
     gene            complement(6152..7756)
                     /locus_tag="VIH70_10600"
     CDS             complement(6152..7756)
                     /locus_tag="VIH70_10600"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY8116852.1"
                     /translation="MRRTSTFAVALILLSATVALAPSAVSAGGAPLWVKHVRNYPGGI
                     SVGVRARLASLDAGTASTASAALQPVPGTDVQMNDDCDPALPQDETSVAVNTSNPLNA
                     VSAANDYCGDGFWIGYTTDGGDSWTSIFKDPKLSPDSLTLADRRCFGSDPSVVYSTRD
                     SAFYLSTLCFAPSNPSSEVQVWRSADGGATWTDSVDAATAITNIAPDGSIDGSVFYDK
                     ELLAVDNNVGSANYGRLYLTFIKFHMTGPSGRSDYCPVQLRTTNPSGAPNTWTWSHTA
                     VVADDPGAKGVGASANQWAMPVVDETGALDIAYTLEDCNSSLDRAFFFTRSTNAGGSF
                     SAPVRVDKAGQFADNPNLQDRLPNKKARMPISPSLVYNPLDNRMLFAYQNNVDRQSSG
                     ADISIQTSANFGATWSDTRPVAVTGSGDAAPGDQFFPWLAVNGSSGDVFAVWFDQRND
                     PTNDLIETFLGESTDGGQTWTNVLISDEAWDPDDAFFNCGCFIGDYNGMALGGSLLYP
                     VWTDGRNSAGKPLGQTDIFTDVIGLT"
     gene            7919..8560
                     /locus_tag="VIH70_10605"
     CDS             7919..8560
                     /locus_tag="VIH70_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HEY8116853.1"
                     /translation="MPARVSVVAISRAAGAEGERIGALVAERLGFRYVDEEIITRAAE
                     KQGVDPAAMADAERRKSVILRVLEGLGQSGAAGATPGGPMWVPDDATELAQSHDRRAL
                     IRETIHETAAQGDVVIVAHAAAFALAGRDDLVRVLVTAPVEIRTRRLAEARDGDQAKA
                     ASELKRSDEARAAYLKTFYGVERELPTHYDLVVNTEVLDPNEAADLIARAAGA"
     gene            complement(8571..10376)
                     /locus_tag="VIH70_10610"
     CDS             complement(8571..10376)
                     /locus_tag="VIH70_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEY8116854.1"
                     /translation="MLAAPAAASGEPGDAGASGATGATVPMVPTLPAGALPGIDVSNH
                     QGAIDWLSVAASGTRFAFAKATEGRTFIDPMYAVNKAGAALSGVVFGAYHFARPDGTA
                     NDAIIEADHFVDVAQPQPGNLIPVLDIERTGDLSQEQLTQWILAWLGRVTERVGVLPM
                     VYTSPHGWATRTGDTSAVADAGYTQLWVAHWGVSSPTVPGGNWGGNGWTFWQYGNCGA
                     IPGIDGCVDVDWYETSSFDPVTIPVPDDVPPSATIALPATPGEPISITFSEIVHRVTP
                     ENVFVWIPSTATYPDVELSCQSGKGRPVDCVAGNVRSARVRPLGPSVLGETYEAVVNP
                     AIAPVLVVDRSANPVPTTALGFATPTEVEDDSPAISYAWRTASKAGAKGGRYAVEHRA
                     GATASFAFAGGSVTWYTAVGPDRGKAVVSIDGVRRGTFDAYAARTDFGVPRTFDGLGP
                     GSHTITIRVLGRARASATDTQVVVDAFRAGSKLVSNPDLRATWGPVERGGASGGSLGA
                     SDLARSSAEVRFRGTGIEWFTYRGTDQGRAEVYVDGLRVRTFDNFAPSPTFDVARSVT
                     GLAEGAHTFRIVVLSTARPAATGKLVSIDRFSVVP"
     gene            10626..10976
                     /locus_tag="VIH70_10615"
     CDS             10626..10976
                     /locus_tag="VIH70_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY8116855.1"
                     /translation="MRPVREPDVMAAALVAVPLVAAKGDSIRLDADIGSILGYVLVGL
                     IVGLLARMLVPGRDPIGLLGTLAIGVVGAVLGGWLAGAVFEETSGVDWIASIGVAILL
                     VLLVRSGSRRRAWR"
     gene            11157..11618
                     /gene="bcp"
                     /locus_tag="VIH70_10620"
     CDS             11157..11618
                     /gene="bcp"
                     /locus_tag="VIH70_10620"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY8116856.1"
                     /translation="MTILRPGDPAPPFELLDQHGSAVRLGDYRGRKLLVYFYPEADTP
                     GCTVQSCDLRDHRQDLAGIGTEVVGISPDLPDKQLAFDEKYKLGFPLLSDPDHATAEA
                     WGAWGEKVRGARTSVGLIRSSFLVDEQGRIAGAWSPVTPQATVPNALAALA"
     gene            complement(11662..>12234)
                     /locus_tag="VIH70_10625"
     CDS             complement(11662..>12234)
                     /locus_tag="VIH70_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY8116857.1"
                     /translation="ATNFTVTAGGFSITSFNPTSGVIGTSVVITGVGFTGANAVRFNG
                     TTATFKVNTPTQITAVVPAGATTGPISVKKPGSHTATSSTNFTVIPPTIHPRTITLDL
                     CRHLVARGLVTANDGFTDCSSNAPIRLQRLRDGRWRTVDTTLTNTDGVYRERIPNRAG
                     TYRAVAIRLGLNDGADVCARDRSPRERYAG"
BASE COUNT         1851 a         4274 c         4223 g         1886 t
ORIGIN      
        1 cggcgccccg gtctcctcgt cgaacagcgc gatcagcgcc tgatgagacg ggagcccccg
       61 gtcgtggttg ccggggaaga ccgcgacgag cttggcctcg agcgtccccg gcaggtaccc
      121 cggcatcgag ccgaggaagc cgttcggggt ccgcgcgacc gtgcgcggcg ggaccgaggc
      181 gttcccggcg ctcagttcgc ggagcgcgac ctccagcgcg tcgaacagca ccggcaggtc
      241 gatgaggttg cggacgtcct cggcgttcag caggagcagc ggcgccatcc gcccgatgat
      301 aggtgggggc gggcgggcca acgacgggcc cgcctgccac ctccggtcta ggcgccgccc
      361 tcgttcagcg tgacctcgat cggctggtag gagtcgccgg tcgtgtcgac gccgagatcc
      421 tgtaggttcg cgagcgcctc gttgacgatg tcgttcgtgt aggcgccggc gtccgggggc
      481 gcggtgagca ccgtcgtgcc ctcaaggttg ggcgtgttct gcgcgatctc ggccgtgcgg
      541 tcccacgcct cctggtcgat gacgcccacg ccgtccggcg cgggccagat cagttggttg
      601 atctcgttca tctgccacag ctgatggctg gcgccgagct tggatccctg cgccaccacg
      661 aggtccgtgc actcctggac gttgtcgcgg cagtacgccc agccctgcag cgacgcggtg
      721 acgaaccgga cggcgatgtc ccggtacgcg tcgtcggact cgagccggtc gccgtccgcc
      781 cagatcgcat cctggagcat cccgaccccg acctcctcgt acgagatcac gttcaggtcg
      841 gacggctggt agagctcgcc ggtgtccggg ttcacggtct cgagcacctg cgcgtactcg
      901 ttgtagatca tcgcctcggc cgcgtcgatg tcgcccgaga gcagcccgga catgtcgaag
      961 tcctgctgca ccagcgtgac gtcggtggcc gggtcgaggt tcgcctgcgc gagcgccgcg
     1021 aagatctcgt actcgttgcc gaacccccag ttgccgatcg tcttgccggc gaagtccgcc
     1081 ggagtcgtga tccccgcctc ggcgaacgag acctgcagcg tgcccgagcg ttggaacacc
     1141 tgcgcgatgt cgacgatgtt cgcgccggcc tcacgggtcg cgagcgcctt gggcacccag
     1201 gcaagcgcga agtccaccgc accgtcggcg agctgctgct gcggcacgat gtctacgccg
     1261 ccctcgacga tcgtcacctc cagacactgg tcggcgtaga acccctggtc ctgggccgcg
     1321 tagtagccgg cgaactgcgc ctggatgaac cattgcagct ggagcgtcac ctggtcggtg
     1381 ctggcacagt ccgcaccacc gcccgcctcg ccgccggctg tcgacccgcc gccgccctcg
     1441 tcgtcgttgc cgcacgccgc cgcgaccagc gagagcgcga gcacgccggc ggcgaggaac
     1501 ctgagccgct tccccctcat ctgggccccc ttccccccgg ggccgatgcc ccgatgaagc
     1561 ctccgtgcga cccgacatca tgccctatcg cgctcccgcg cgccgacgcc gccgggcgtg
     1621 gccacccgct cgagcgccgc ggcggtgagg aagaacgcga gacccagcag gcacgccccg
     1681 aggacgtacg cccaggcctc cgcgttgttc gacagcgcga tgttgctcac gatccggctg
     1741 ccgagcccgt tctgcagccc gccgaagtac tcggagacga acgcggtgat caccgaggcc
     1801 ggcgcggcga tcctgagcgc ggtaaagagg taggggacgg cgtgcggcac ccggaccttg
     1861 cgcaggaccg tgacgtcgct cgcggcgtac gaccgcatca ggtctaggta caccgagccc
     1921 acctcccgca ggcctcgggc gacgttgatc aggacgatga agtagacgac cagggtcgcc
     1981 atcaacctgc gcggcacctc gctcgtcacc gggtacatgt tgttgaacac ggcgaccagg
     2041 acgaagatcg ggatcgcgtt cagcgccacg gcgaggggcg acatcaggtc gtcggccagc
     2101 cggaagcgcg cgagcaggaa cgccatcccg accccgatcg ccgtcccgag gacgagcccg
     2161 atcagggcgt tcgagcccga gaccttggtc gcctcccaga tcagcgccgt gttcgaccgg
     2221 aacgcgtccc agatcgccga gggggccggc aggaagtacg gctggaagtc gaagacgttc
     2281 accgctgtct cccagatcgc gaggaacgcg acgccgaaca ccaccggcgg ccagatcagg
     2341 ccgaacgggt ggcggcgcga acgggtcacg gctcctgggc catctgctcg atgtccggct
     2401 cgatgccggc cggttggatg cctcgcagcg cctcccgcac ctcggtgacc ttggcgaaga
     2461 aggcctcggt ctcgcgcgta tccacgctgc gtcgcgggcc gagctccacg tcgaccacgt
     2521 cggtgatcct ccccgggcgc ggcgacatca cgaccacccg cgtcgacagg aacaccgcct
     2581 cggggatcga gtgagtcacg aacaccaccg tcgtcgaggt ctccccgcag atccggagca
     2641 gctccgtctg catgtgctcg cgcgtcatct cgtcgagcgc cccgaacggc tcgtccatca
     2701 gcagcaacgg cgggtgggcg gcgagcgccc gcgcgatcgc cacccgttgc tgcatgccgc
     2761 cggacagctg ccacgggaag tgctcggcga actccggcag cttgaccagg tcgagcatct
     2821 cgagcgcgcg ggcgcgccgc ctggccttgt cccacccctt cagttcgagg ggaagttcca
     2881 cgttcttggc gaccgatcgc cactcgaaca gaccggactg ctggaacgcc atgccgtagt
     2941 cctggtccag cctcgcggtc cgcgccggct tgccgttgac caggacctcg cccgtcgtgg
     3001 gttcggtcag gttggcgatc aggcggagct gggtcgactt gccgcatccc gacgggccga
     3061 tcagggagac gaactcaccg ggcgcgatga tcagatcgat gtcggcgagg gcctcgacgg
     3121 cgtccgggcg tccggcgttg aacaccttgg tcaggccgcg gacctcgaca gcactcgtgc
     3181 cgtcgccgcc ggccggcgcc gagttcactc catcgcctcg cgcggccggt tgcgcatccc
     3241 cacgaggtcg accagcgcga cgatccccgc catcacgagc ccgagcgcgg ccgccccgaa
     3301 cacggccgtg aacaccttcg cgggatcgct ggtcgcctcc ctcgcgtact cgatgatcag
     3361 cctgccgatg ccaccggcca gaccggtcga gatctcggcg acgacgacgc cgacgaccga
     3421 cgcggtcgcg gccagcttga gggcagggac gatgtacggc agcgcggccg ggaacctgag
     3481 cttgaacagg gtctggcgcc aggacgccgc gtacgagtcc atgagctcga gcgacgcggg
     3541 tggcgtcgag gtcaacccct tcagcgtgcc gacggcgacg gggaagaagg cgaggaaggc
     3601 gccgagcacg gccgccgaga gccaccgcgg ccactcgaac gcgccgacgt gcagccggcc
     3661 tccccagctc acgacgagcg gcgcgagcgc gatcaacggc acggtttgcg agaggacgag
     3721 gtacgggagg agcccccgtt cgacgacctt gtaccgcgcc atcagcaccg cgagcccgag
     3781 cccgaccgcc gtcccgagcg cgaggccggc gagcgcgatc cggaacgtga accaggctcc
     3841 ccgcaacacg accaccagga tcggcgtgtc ggacgcgcgg ttctcgggct gcccgtagcg
     3901 cgagaccatc tcccatacgt ggggcatcgc ggtgtcgttc gcgcggggca ggatcgtcca
     3961 gccgaacacg gtcccgccgt cctcgggacc gatgaccttg tagagctccc acacgacggc
     4021 caccaggatc agcgcgagcg cgaacgtcca gacgcgccgg aaccgcgtca actcttcgcg
     4081 cggaccgtgt cggcgatggc cgggatgacc ttctcgccgt aggcctgcag ggtctcgtcc
     4141 ttcgcgtcgt gttgcaggta gacggcgaac tggtggacgc cgagcgcctt cagctcctcg
     4201 aggcgtcgca cgtgctcctc gaccggcccg aggacgcaga agcgatcgat gatctcgtcc
     4261 ggcacgaact ccgtgtggac gttgcccgcc tggccgtgct cgttgtagtc gtagccctcg
     4321 cgacccttga tgtagtcggt gagctcctgc ggtacggcgg aggtctcgcc gtagcgagcc
     4381 acgatgtcgg ccacgtggtt gccgaccatg ccgccgaacc accggcactg gtcgcgggcg
     4441 tgtgcgacat cgtcggtgac gtacgccggc gcggcaacgc agatcatgac gtcgtcggga
     4501 tcgcgtccgg cctcctcggc cgccgtgcgg acggccccga tcgtccactc cgtgatgtgc
     4561 gggtcggcga gctgcaggat gaacccgtcg cccacctcgc cgatcagctt gagcgccttc
     4621 ggcccgtacc ccgcgaccca tacctcggcg cggctcctcg acgcccacgg gaaccggatc
     4681 ctcgaacccc ggtactcggc cgtgcgaccg ttgacgagct cgcggatcac ctggacgctc
     4741 tcgcgcagcg tcgcgagggt ggtcggcttg ccgttgatga cccgcacggc cgagtcgccg
     4801 cgcccgatgc cgatcaccgt ccggttcccg aacatctcgt tcagcgtggc gaagacgctc
     4861 gccgtcaccg tccagtcgcg ggtcgccgga ttggtgacca tcgagccgac ggtcaccttg
     4921 cgggtgttcg cgaggatctg cgtgtagatc gggaacggct cctcccacag gatgtggctg
     4981 tcgaacgtcc acacgtgcgt gaacccgtag gtctccgcct tggtgccgag gtcgatcacg
     5041 cgggcgctcg gaggggtggt ctgcagcacc acaccgacgt ccatcgcgcc cctcctcttc
     5101 ttccctttct aggcccgtcg ctcagcgagc gacgatcggg tcgtacgcgt cgggccggcg
     5161 gtcccggtag aacgcccacc ggtcgcggac ctcgcggatc ttgtccaggt cgagctcgcg
     5221 gacgatcagc tcgggcttgt acgcgtcgcc gacctcgccg acgaactgtc cctccggatc
     5281 gacgaagtag ctctgcccgt agaagtcgtt ctcgccgagc ggctcgatgc cgacgcggtt
     5341 gatcgcgccg acgtagtaca tgttcgcgac ggcggcggcc ggctgctcga gccgccagat
     5401 gtactcgctc agtccgcgat gggtggccga cgggttgaac acgatctcgg cgccgtgcag
     5461 tccgaggatc ctccacccct ccgggaagtg ccggtcgtag cagatgtaga cgccgacctt
     5521 gccgacggcg gtctcgaaca ccgggtaccc ctcggtgccg ggtgtgaagt agaacttctc
     5581 ccagaaccct ttcacctgcg ggatgtgctg cttgcggtac ttgccgaggt aggtcccgtc
     5641 ggcgtcgacc acggccgcgg tgttgaagta ctgccccgcc tgcacgaact cgtacatcgg
     5701 gagcaccatc accatcccga gctccttcgc gacgctctgg aaccgcttgg tggtgggacc
     5761 atcggggatc ggctcggtgt agctgtagta ctcggtgtcc tggacctggc agaagtaggg
     5821 cccgtagaac agctcctgga agcagatgac ctcggcgccc tgcgccgagg cctcccgagc
     5881 ggcctcctcg tgcttgtcgg tcatggactc ctggtcgccg gtccagtccg tctggagcag
     5941 tgcggccttg acggtcctcg acatcgcgcc ctcctgaggg ttcatgccgg cgcccgacgc
     6001 gcgccgattc ccccggatgg taaccaccct ccgtccggag cgcgaggggt gaggtggccg
     6061 gacttcggtg ccgaccctca ggccgtcggc tccacgaccc accccctcgt cggtccacga
     6121 cgacgatgag gagccgcggg agcgtgttcg gctaggtcag gccgatcaca tccgtgaaga
     6181 tgtccgtttg ccccagcggc ttgcccgccg agttccgccc gtcggtccag acggggtaca
     6241 gcagcgaacc gccgagggcc atcccgttgt agtcgccgat gaagcatccg cagttgaaaa
     6301 aggcgtcatc gggatcccac gcctcgtcgc tgatcaggac gttcgtccac gtctgaccgc
     6361 cgtcggtcga ctcgccgagg aaggtctcga tcaggtcgtt cgttgggtcg ttccgctggt
     6421 cgaaccacac cgcgaacaca tcgccggacg acccgttgac cgcgagccac gggaagaact
     6481 gatcgccggg tgccgcgtcg cccgaaccgg tgacagcgac cggacgtgtg tccgaccacg
     6541 tggctccgaa gttggccgag gtctggatcg agatgtctgc tccggagctc tggcggtcga
     6601 cgttgttctg gtaggcgaac agcatccggt tgtcgagagg gttgtacacg agcgagggtg
     6661 agatcggcat ccgagccttc ttgttcggca gccggtcctg aaggttcggg ttgtcggcga
     6721 actgtcccgc cttgtccacc cggaccggag ccgagaagct cccgccggcg ttcgtcgacc
     6781 gagtgaagaa gaaggcgcgg tccaaactcg agttgcagtc ctccagggtg tacgcgatgt
     6841 cgagcgctcc cgtctcgtcg acgacgggca tcgcccactg gttggcggac gcgccgacgc
     6901 ccttggctcc ggggtcgtcc gccacgacgg cggtgtgcga ccacgtccag gtgttgggag
     6961 caccgcttgg gttcgttgtt cgcagctgga cgggacagta gtcgctccgc ccggacggcc
     7021 cggtcatgtg gaacttgatg aacgtgaggt acagacgccc gtagttcgcg ctcccgacgt
     7081 tgttgtcgac cgccagcaac tccttgtcgt agaagaccga cccatcgatc gagccgtcgg
     7141 gagcgatgtt cgtgatcgcc gtcgccgcat ccacggagtc cgtccaggtg gcgccgccgt
     7201 ccgccgagcg ccacacctgc acctcggaac tcgggttgga cggggcgaag cacagggtcg
     7261 acaggtagaa cgccgaatcg cgggtgctgt acacgaccga cggatccgag ccgaagcaac
     7321 gccggtcggc gagggtcagc gaatccggcg agagcttcgg gtctttgaag atgctcgtcc
     7381 aggagtcgcc gccgtccgtc gtgtatccga tccagaagcc gtcaccgcag tagtcgttgg
     7441 ccgccgaaac cgcgttcagc gggttgctcg tgttcaccgc caccgaggtc tcgtcctgcg
     7501 ggagcgcagg gtcgcaatcg tcgttcatct ggacatcggt tccgggcacc ggctgcaggg
     7561 ctgcggaagc ggtgctcgcc gtcccggcgt cgagcgatgc gaggcgcgct cgaactccca
     7621 ccgagatgcc cccgggatag ttccgcacgt gtttgaccca caggggggca ccaccagcgc
     7681 tcacggccga tggcgcgagg gcgacggtgg ccgagagcag gatcagggcg accgcgaacg
     7741 tcgaagtccg acgcatgaca cgcctccttc gcaggggttg tctccacccc atcgcacctc
     7801 ggcgcgctct gtcaaggccg cacgtgaact ctcatcaccc ccgatcggcg gtacggtgtc
     7861 ctcttcgatc gaggacagac cacgtacacg gcgcccaccg cgggcgcggg aggtaaccat
     7921 gccggcacgc gtgagcgtcg ttgcgatctc gcgggcggcg ggcgccgagg gcgaacggat
     7981 cggcgcgctc gtcgccgagc ggctcggctt ccggtacgtc gacgaagaga tcatcacgag
     8041 ggccgccgag aagcagggcg tcgaccccgc cgcgatggcg gatgccgaac gccgtaagag
     8101 cgtgatcctt cgggtcctgg agggcctcgg ccagagcggc gccgccggcg cgacgccggg
     8161 cgggccgatg tgggtccccg acgacgcgac cgagctcgcc cagagccacg accggcgcgc
     8221 tctgatccgc gagacgatcc acgagaccgc ggcgcagggc gacgtggtga tcgtcgcgca
     8281 cgccgcggcg ttcgcgctcg cgggccggga cgacctggtg cgcgtgctgg tcacggcgcc
     8341 ggtggagatc cggacccgac ggctggcgga ggctcgcgac ggtgaccagg cgaaggccgc
     8401 ctcggagctc aagcgctcgg acgaggcccg cgcggcgtac ctcaagacct tctacggcgt
     8461 ggaacgcgag ctcccgaccc actacgacct cgtcgtgaac accgaggtcc tcgatccgaa
     8521 cgaggcggcg gacctcatcg cgagggccgc cggcgcgtga cggatcggcg ctacgggacg
     8581 accgagaacc ggtcgatcga caccagcttg cccgtggccg cgggtcgagc ggtcgagagc
     8641 accacgatcc tgaacgtgtg cgcaccctcg gccagcccgg tgaccgagcg cgccacgtcg
     8701 aacgtcggtg acggcgcgaa gttgtcgaac gttcggaccc gcagcccgtc cacgtagacc
     8761 tcggcccgac cctggtcggt cccgcgatac gtgaaccact cgatgccggt cccacggaac
     8821 ctgacctccg cgctcgaacg cgcgaggtcg ctcgccccca ggctcccgcc ggacgccccg
     8881 ccccgctcga cgggtcccca ggtcgcgcgc aggtcggggt tggagaccag cttgctgccg
     8941 gcgcggaacg cgtcgacgac gacctgcgtg tcggtcgcgc tcgcgcgcgc gcgaccgagc
     9001 acccggatcg tgatcgtgtg tgagccgggt cccagcccgt cgaaggtccg cgggaccccg
     9061 aagtccgtcc gcgcagcgta cgcatcgaac gtgccgcgcc gaacgccgtc gatcgagacc
     9121 acggccttgc cccggtccgg ccccacggcc gtgtaccagg tgaccgatcc gcccgcgaag
     9181 gcgaacgagg cagtcgcgcc ggcccggtgc tcgacggcgt agcgaccgcc cttggcgccg
     9241 gctttggagg ccgtgcgcca cgcgtaggag atcgcggggc tatcgtcctc gacctcggtg
     9301 ggcgtcgcga accccaaggc ggtggtcggg accgggttcg cgctccgatc caccacgagc
     9361 accggcgcga tcgccgggtt cacgacggcc tcgtaggtct cacccagcac gctcggtccg
     9421 agcggccgca cgcgcgcgga acggacgttg cccgccacgc agtcgacggg acgccccttg
     9481 cccgactggc acgagagctc gacgtcgggg taggtcgcgg tgctcgggat ccacacgaac
     9541 acgttctccg gcgtcacccg gtgcacgatc tcgctgaacg tgatcgagat cggttcgccc
     9601 ggcgtggcgg ggagcgcgat cgtggcggac ggcggcacgt cgtcgggcac cgggatcgtg
     9661 acggggtcga acgagctggt ctcgtaccag tccacgtcga cgcacccgtc gatcccgggg
     9721 atggctccgc aattgccgta ctgccagaac gtccacccgt tgccgcccca gttcccaccc
     9781 ggcacggtcg gcgacgacac cccccagtgc gccacccaca gctgcgtgta gccggcgtcg
     9841 gcgacggcgc tcgtgtcgcc ggtccgcgtc gcccatccgt gcgggctcgt gtagaccatc
     9901 ggcaggacgc cgacccgctc ggtcacgcgg cccagccagg cgaggatcca ctgcgtcagc
     9961 tgctcctgcg acaggtcgcc ggtgcgttcg atgtcgagga ccgggatcag gttgccgggc
    10021 tggggttgcg cgacgtcgac gaagtggtcg gcctcgatga tggcgtcgtt cgcggtgccg
    10081 tcgggccgcg cgaagtggta cgcgccgaac accacgccgg acagggcggc gcccgccttg
    10141 ttgacggcgt acatcggatc gatgaatgtc cggccctcgg tggccttggc gaacgcgaac
    10201 cgagtgccgg aggccgcgac cgacagccag tcgatcgcgc cctgatggtt cgagacgtcg
    10261 atgcccggga gcgcccccgc ggggagcgtc ggaaccatcg gcacggtcgc gcccgtcgcc
    10321 cccgacgcgc cggcatcacc cggctcgccg ctggccgcgg ccggagcggc gagcacgagg
    10381 atcaggacga cggtcgcgaa ggcggtcgcg ggcgccgtcg atcgtcgtcg agacctcgtc
    10441 gttgttctcc ccccggtgtg cgagaccgcg gaggctatcg gatggttcca cgaacgtcga
    10501 cccgatcccg gggcccgccg caacctgacg gagttcggac gtcggatcgg agctcgggca
    10561 gtcaagattg aacgggtgcc cggccgggta caccaccttg gacgagagac gagggaggta
    10621 cacccatgag accggtcagg gaaccggacg tgatggcggc ggcgctcgtc gccgttccgc
    10681 tggtcgcggc gaagggtgac agcatccgcc tggatgccga catcggctcg atcctcggct
    10741 acgtcctcgt agggctgatc gtgggtctgc tcgctcggat gctggtgccg ggccgagacc
    10801 cgatcggcct gctgggcacg ctcgccatcg gcgtcgtggg tgccgtgctg ggtggctggc
    10861 tcgccggcgc cgtcttcgag gagacgtcgg gggtcgactg gatcgcctcg atcggcgtgg
    10921 ccatcctgct cgtcctgctc gtgcgctccg gctcacggcg cagggcctgg agatagcgga
    10981 cggggatcgg accgcgggac ccaccgcgac gctgcgagcg atggggcccg cggagatcct
    11041 gacctacctc gagatcggcg cgaagcgcac gttcgccggc gccctcgact tgcccgggtg
    11101 agctttcggc gcgagcgggg cccatcgcaa ccgcgtcaca cgggtaccgt agaagcgtga
    11161 ccatactgag gcccggcgac ccggcgccgc ccttcgagct cctggaccag cacggttccg
    11221 ccgtacgcct cggggactac cgtgggagga agctcctcgt gtacttctac cccgaggcgg
    11281 acacacccgg gtgcaccgtc cagtcgtgcg acctccggga tcaccgacag gacctcgccg
    11341 gcatcggcac ggaggtcgtc gggatctcgc ccgatctgcc cgacaagcag ctcgcgttcg
    11401 acgagaagta caagctcggg ttcccgttgc tctcggaccc cgaccacgcg acggcggagg
    11461 catggggcgc gtggggcgag aaggtccggg gcgccaggac ctcggtcggg ctcatccgct
    11521 cgtcgttcct cgtggacgag cagggccgga tcgcgggcgc ctggtcgccg gtgacgcccc
    11581 aggccacggt cccgaacgcg ctcgccgcgc tcgcctgagt tgcggtagcg aatcggtcga
    11641 tccgacccgc cgaggttgtt cttagccggc gtaacgctct cggggtgatc ggtcgcgggc
    11701 gcacacgtcg gcgccgtcgt tcagtcccag ccggatcgcg acggcgcgat acgtccccgc
    11761 gcggttcggg atgcgctccc ggtacacgcc atctgtgttc gtcagcgtcg tatcgaccgt
    11821 ccgccaacgc ccgtcacgca ggcgttggag cctgatcggt gcgttggagg aacagtccgt
    11881 gaacccgtcg ttcgccgtca ccaggcccct ggccaccaga tgtctgcaga gatcgagcgt
    11941 gatggtcctc gggtggatcg tgggcgggat cacggtgaag ttggtcgaac tcgttgcggt
    12001 gtggctgccg ggcttcttca cggagatggg gccggtcgtc gcccccgccg ggacgacggc
    12061 ggtgatctgc gtaggagtgt tcaccttgaa cgtcgccgtg gttccgttga agcggaccgc
    12121 gttggccccc gtgaagccga cgcccgtgat gaccaccgag gttccgatca cgccgctcgt
    12181 cgggttgaac gatgtgatcg agaagcctcc ggccgtcacg gtgaagttgg tcgc
//
LOCUS       DATBRK010000356         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1224927, whole genome shotgun sequence.
ACCESSION   DATBRK010000356 DATBRK010000000
VERSION     DATBRK010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224927"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2567
                     /gene="carB"
                     /locus_tag="VIH70_10630"
     CDS             <1..>2567
                     /gene="carB"
                     /locus_tag="VIH70_10630"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY8116858.1"
                     /translation="VIGQACEFDYSGTQACKVLRREGFHVSLVNSNPATIMTDPEFAD
                     RTYVEPLTPESVLRVIERERPDVLLPTLGGQTALNVAVALAESGDLERLAVGLIGAGL
                     DAIHRAEDRSGFKETMNRAGLKTPRAGTARSLDRAVELGDELGYPLIVRPSFTLGGGG
                     SGFARNRAELERVASRSLAASPVTEILVEESVAGWKEFELEVMRDGADNAVVICSIEN
                     VDPMGVHTGDSITVAPVMTLSDREYQWMRDAALACIRAVGVETGGSNIQFAVDPRTGR
                     QVLIEMNPRVSRSSALASKATGFPIAKIAALLAVGYTLDEIPNDITGVTLAAFEPTLD
                     YVVVKVPRFAFEKFPEADPELTSTMKSVGEVMAIGRTFKEALGKAWRSLERPDVDLGG
                     PGAGDGGRPLDALATPSEHRLHLVEAALRGGSSVEEVALASGIDPWFVDQIAQVAERV
                     AALDGRELGTVAAPELERAKRIGLSDRRLARLTSSTEDEVRRRRETLGVRPVFRTVDT
                     CAGEFPARTPYHYSAYEEQDEVRPAERPRVVILGAGPNRIGQGIEFDYACVHAAFALG
                     DAGFEAVMINSNPETVSTDYDTSSRLYFEPLSTEDVLAVCEAERPIGVICQFGGQTPL
                     RMARELEAAGFPVLGTPASKIDLAEDRGKFAGLLLELDIAAPEHGEANDLVQARAIAT
                     RIGYPVVVRPSYVLGGRAMEIVDGDDELERFVAQAAAAGPDHPVLIDRFLEGAIEIDV
                     DAVSDGTDVFIGAVMEHIEEAGIHSGDSSCQIPPATLSDDELDEVERITRSLALRLEV
                     RGLINLQLASKDERIWVLEANPRASRTVPFVSKVTGVPLAKVATSVMLGRTLAELRD"
BASE COUNT          374 a          859 c          965 g          369 t
ORIGIN      
        1 gtgatcgggc aggcgtgcga gttcgactac tcggggaccc aggcctgcaa ggtgctgcgg
       61 cgggaggggt tccacgtgtc gctcgtgaac tcgaacccgg ccacgatcat gaccgatccg
      121 gagttcgcgg accgcacgta cgtcgaaccc ctgacgcccg agtcggtcct gcgggtgatc
      181 gaacgcgagc gccccgacgt gctgctgccg accctcggag gtcagaccgc gctgaacgtc
      241 gcggtcgcgc tcgccgagtc cggggacctc gagcggctcg cggtcggact gatcggggcc
      301 gggctggacg cgatccaccg ggccgaggac cggagcggct tcaaggagac gatgaaccgc
      361 gccgggctga agacgccgcg cgcggggacc gcgcgctcgc tcgaccgcgc ggtcgagctc
      421 ggggacgagc tggggtaccc gctgatcgtc cggccgtcgt tcacgctcgg cggcggcggg
      481 agcgggttcg cccgcaaccg cgccgagctc gagcgcgtcg cgagccgcag cctcgcggcg
      541 agcccggtga ccgagatcct ggtggaggag tcggtcgccg ggtggaagga gttcgagctc
      601 gaggtgatgc gcgacggcgc ggacaacgcc gtcgtgatct gctcgatcga gaacgtggac
      661 ccgatgggcg tgcacaccgg cgactcgatc acggtcgcgc cggtgatgac gctgtccgat
      721 cgcgagtacc agtggatgcg ggacgccgcg ctcgcgtgca tccgcgctgt gggggtcgag
      781 accggcgggt cgaacatcca gttcgcggtc gacccgcgta ccggccgtca ggtgctgatc
      841 gagatgaacc cgcgggtctc gcgttcgtcg gcgctggcct cgaaggcgac gggcttcccg
      901 atcgccaaga tcgcggcgct cctcgccgtg ggctacacgc tggacgagat cccgaacgac
      961 atcaccggcg tgacgttggc tgcgttcgag ccgacgctgg actacgtcgt ggtgaaggtg
     1021 cccaggttcg cgttcgagaa gttccccgag gccgacccgg agctgacctc cacgatgaag
     1081 tccgtcggcg aggtgatggc gatcggccgg acgttcaagg aggcgctggg caaggcctgg
     1141 cgctcgctcg aacgtcccga tgtcgatctc ggcggaccgg gtgccggcga cggcggccgg
     1201 cccctcgacg cgctcgcaac accgagcgag caccggctgc acctcgtgga ggcggccctg
     1261 cgtgggggga gctcggtcga ggaggtcgcg ctcgcgagcg ggatcgatcc gtggttcgtc
     1321 gaccagatcg cgcaggtggc cgagcgggtc gcggcgctcg acgggcgcga gctcgggacg
     1381 gtcgcggccc ccgagctcga gcgggccaag cggatcgggc tgtccgatcg gcggctcgcg
     1441 cgcctcacgt cctcgaccga ggacgaggtc cgcaggcgcc gggagacgct cggggttcgg
     1501 cctgtgttca ggacggtcga cacgtgcgcc ggcgagttcc ccgcccgcac cccgtaccac
     1561 tactccgcct acgaggagca ggacgaggtg cgaccggccg aacgtccgcg cgtggtgatc
     1621 ctcggggcag gtcccaaccg gatcgggcag gggatcgagt tcgactacgc ctgcgtccac
     1681 gcggcgttcg cgctcgggga cgcggggttc gaggccgtga tgatcaactc gaaccccgag
     1741 accgtctcca ccgactacga cacgagctcc cgtctgtact tcgaaccgct gtcgaccgag
     1801 gacgtcctgg cggtgtgcga ggccgagcgg ccaatcggcg tgatctgcca gttcggcggg
     1861 cagacgccgc tgcggatggc gagggagctc gaggccgccg ggttcccggt cctcgggacg
     1921 ccggcgtcca agatcgacct cgccgaggat cgcgggaagt tcgccggcct cctcctcgag
     1981 ctcgacatcg ccgcgcccga gcacggcgag gcgaacgacc tcgtgcaggc ccgggcgatc
     2041 gcgacgcgga tcgggtaccc ggtcgtcgtt cgaccgtcct acgtgctcgg ggggcgtgcg
     2101 atggagatcg tcgacggcga cgacgagctc gagcgtttcg tcgcgcaggc cgccgcggcc
     2161 ggccccgatc acccggtcct gatcgaccgg ttcctcgagg gcgcgatcga gatcgacgtg
     2221 gacgcggtga gcgacggcac ggacgtgttc atcggcgccg tgatggagca catcgaggag
     2281 gcggggatcc actcgggcga ctcctcgtgc cagatccctc ctgccacgct gtccgacgac
     2341 gagctcgacg aggtcgaacg catcacgcgc agcctcgcgc ttcggctgga ggtgcgcggc
     2401 ctgatcaacc tgcagctcgc gagcaaggac gagcggatct gggtcctcga ggcgaatccg
     2461 cgcgcgtcgc gtaccgtgcc gttcgtgtcc aaggtcaccg gggtgccgct cgccaaggtg
     2521 gcgacgtccg tgatgctggg tcggaccctg gccgagctcc gggacga
//
LOCUS       DATBRK010000357         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1655841, whole genome shotgun sequence.
ACCESSION   DATBRK010000357 DATBRK010000000
VERSION     DATBRK010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655841"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1969)
                     /gene="recG"
                     /locus_tag="VIH70_10635"
     CDS             complement(<1..1969)
                     /gene="recG"
                     /locus_tag="VIH70_10635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY8116859.1"
                     /translation="MNDVRTKEPRARRLTLESPVGAIDRQLAGRRVGLRTKGPPASEV
                     LADQGIGTVRDLLHHYPRRYIDRSQVSRIGELKIGTYATVIARVKRVTKRQTRRRQSM
                     VTITLYDGSGYLDLTFFNQPWTGSMYKEGYEVAVSGIAQLYRGRLQLANQEIELLGGD
                     DRDLVHTGRITPIHRASEGITTRTIRELVWRALGRLGPIPDPMPEDLIRAEALPPFDR
                     AIRDIHFPGSQPELAAAIERLKFDELFVLELGLAFRKHRVERAEIGVAHVTGGPLLER
                     LDRTLPFEPTAAQHRAMGEIDAAMARPRPMNVLLQGDVGSGKTLVAVHAALVAIGSGH
                     QAAIMAPTEVLAGQHFRQVETLLGGSGAVPYLELASPGSANAAQGSLLDPSPEANDES
                     RVTFALLTAAVTGKDRARILGGIAAGDVDLVVGTHALVQEGVAFADLSLAVIDEQHRF
                     GVHQRMALKGKGASPDVLIMTATPIPRTLALTYYGDLDVVVLDEMPKGRQPIRTLIAR
                     TPPQRQAAYELVRAEVEAGRQAFVVCAAIDETNRNQVRAAEGEAQRLAADVFPDLSVQ
                     VLHGKLRPAEKERVMDGFRSGRTHLLISTTVIEVGVDVPNATVMLVENAERFGLAQLH
                     QLRGRIGRGTHPSTCVLFDESEEDNLEARARL"
BASE COUNT          269 a          720 c          692 g          308 t
ORIGIN      
        1 ccagccgcgc gcgcgcctcg aggttgtcct cctccgactc gtcgaacagg acgcaggtcg
       61 acgggtgcgt cccccgtccg atccggccgc ggagctggtg cagctgcgcg aggccgaagc
      121 gctcggcgtt ctcgacgagc atcacggtcg cgttcggaac gtccacgccg acctcgatca
      181 ccgtggtcga gatcagcagg tgcgtgcgtc cggagcggaa cccgtccatc acgcgttcct
      241 tctcggccgg gcgcagcttg ccgtgcagca cctggacgga cagatcgggg aagacgtcgg
      301 ccgccaggcg ttgcgcctcc ccctccgcgg cgcggacctg gttccggttc gtctcgtcga
      361 tcgccgcgca caccacgaac gcctgccgcc ccgcctcgac ctcggcccgg acgagctcgt
      421 aggcggcctg ccgttgcggc ggcgttcgag cgatcagcgt tcggatcggt tggcgcccct
      481 tcggcatctc gtcgaggacc acgacgtcca gatcgccgta gtaggtgagc gcgagcgtcc
      541 gtgggatcgg ggtcgccgtc atgatcagga cgtccggcga cgcccctttg cccttgagcg
      601 ccatccgctg gtggacgccg aaccggtgct gttcgtcgat gacggccagc gagaggtccg
      661 cgaacgccac accctcctgc acgagggcat gcgttcccac aacgaggtcg acgtccccgg
      721 cagcgatgcc gcccaggatc cgggctcggt ccttcccggt caccgcggcc gtgaggaggg
      781 cgaaggtcac gcgtgactcg tcgttcgcct ccggactcgg atccagcagc gagccctggg
      841 ccgcgttcgc gctcccgggc gacgcgagct ccaggtacgg gaccgccccc gaaccaccga
      901 ggagcgtctc gacctgacgg aaatgctgtc ccgccagcac ctcggtgggg gccatgatcg
      961 cggcctggtg gcccgacccg atcgcgacga gggctgcgtg caccgcgacg agcgtcttgc
     1021 cggaacccac gtcgccctgg agcaggacgt tcatcggtcg cgggcgcgcc atcgccgcat
     1081 cgatctcccc catcgcccgg tgctgcgcgg cggtgggctc gaacggcagc gtccggtcga
     1141 gccgctcgag cagcggtccg cccgtcacgt gcgccacgcc gatctcggcg cgctcgaccc
     1201 gatgctttcg gaacgcgagc cccaactcga gcacgaacag ctcgtcgaac ttcagtcgtt
     1261 cgatcgcggc ggccagctcg ggctgcgagc ccgggaagtg gatgtcccgg atcgcgcggt
     1321 cgaacggcgg cagcgcctcg gcgcggatca ggtcctcggg catcgggtcg gggatcggac
     1381 cgagccgtcc gagcgcgcgc cacacgagct cgcggatcgt ccgcgtcgtg atgccctcgg
     1441 acgctcggtg gatcggcgtg atccgcccgg tgtgcaccag gtcccgatcg tcgcccccga
     1501 ggagctcgat ctcctggttc gcgagctgga gccgtccccg gtacagctgg gcgatgcccg
     1561 agaccgcgac ctcgtacccc tccttgtaca tcgagcccgt ccagggctgg ttgaagaacg
     1621 tcaggtcgag gtagcccgag ccgtcgtaca gcgtgatcgt gaccatcgac tgccgacgcc
     1681 gggtctgccg cttggtgacc cgcttcacgc gcgcgatcac ggtcgcgtag gtgccgatct
     1741 tgagctcccc gatccgcgac acctgggagc ggtcgatata gcggcgcggg tagtggtgca
     1801 ggaggtcgcg gacggtcccg atcccctggt cggccaggac ctcggacgcc ggggggccct
     1861 tcgtcctcag gccgacccgg cgcccggcga gctggcggtc gatcgcgccc acggggctct
     1921 cgagcgtgag gcgccgggcg cgaggctcct tggtccggac gtcgttcacg ccgtcggttc
     1981 taccatgcg
//
LOCUS       DATBRK010000358         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1076455, whole genome shotgun sequence.
ACCESSION   DATBRK010000358 DATBRK010000000
VERSION     DATBRK010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076455"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VIH70_10640"
     CDS             complement(<1..1090)
                     /locus_tag="VIH70_10640"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8116860.1"
                     /translation="MRKLACDPTLLFHVGVPDHASGAIAATSLATPSAHLAQVRGEGP
                     WPEESVEQAVAWEDGLITYVGPADGLAGVEPEWFEGCTIVPGFVDCHTHLPFAGWRAD
                     EFEARLAGVSYRDLHGRGAGGSWSEREGASGRPPGKGGIYRSARMLAAASDDEVLAFC
                     RSLVQEMAEHGTTALELKTGYGLSVEAELRQARLARRLSEEAPQTCSVTLLACHAVPE
                     GMERADWVRVVCDELIPAAARERLVDAVDVYVEDIAFSVDDLAEVARCASDVGLPLRC
                     HADQLGPSGAAEAAVALAARSADHLNHVSATGIAALGGAPATVAVLLPTSTLFLGSSP
                     PDVRALLDAGAAVAIATDFNPGTSPVLSM"
     gene            1150..2370
                     /locus_tag="VIH70_10645"
     CDS             1150..2370
                     /locus_tag="VIH70_10645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8116861.1"
                     /translation="MSEAERFRIREVLAIPEIRAAMLGTFVIMLGYGIISPVLPNYAR
                     SFGVGYDSIGLLISSFSFARLVSDPFVGRYIDRYGERGMATLGAAWVGVSSFAAALAP
                     TFPLLVLFRALGGVGSALFFASLLSFLLRSIPSHRTGRVMSVYYASFNIGFIAGGPLG
                     GLIAKWFTLATPLYVYAGACFLAAALFWRTIRDPERHESEVRRGGIRRLPWNRAFVTV
                     LVVNGVYLWMIGAVFSTLVPLFGTSHEVGLTIGGVGIGLAIATATELACLYPAGHATD
                     RRGRRAVLIPALAGLALITAGFGFVSTPVGFMTAMALLGVASGYSGVPPAPMLSDVTP
                     EELKGSAVAVFRFVGDVGFVIGPLVAGWSAQAYGFTASFAINAIPAVVALGLVLSIRE
                     TMHARRASDESGGL"
     gene            2373..2810
                     /locus_tag="VIH70_10650"
     CDS             2373..2810
                     /locus_tag="VIH70_10650"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8116862.1"
                     /translation="MRVQAFRDLAASPERVWSSIERWEEQSRWIRDAAWVRVLTPERT
                     GVGARIEVLSRVLHVPLFTEQLEVVGWEPPRTVVMAHRSFVRGTGTWSLERVDGSTRF
                     SWTEELSLPIPVLGELALLVYRPFLRRLMRRSLARLQRLVAST"
     regulatory      2849..2988
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2978..>3301
                     /locus_tag="VIH70_10655"
     CDS             2978..>3301
                     /locus_tag="VIH70_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY8116863.1"
                     /translation="MSRVAERLEKILRREPPAWGIRPVPLERRNLGGLDLAVLWFDLS
                     VGLLVMVTGALLVPALGTGRAFLAIGVGTLIGCVPLALVGLAGEREGVPTMVLLRPVL
                     GARGSF"
BASE COUNT          394 a         1175 c         1214 g          518 t
ORIGIN      
        1 gcatcgagag caccggcgag gtgccggggt tgaagtcggt cgcgatcgca accgccgcgc
       61 cggcgtcgag cagggctcgg acgtccggcg gcgacgagcc caggaacaac gtcgacgtgg
      121 gcagcaggac cgcgacggtc gccggggcgc caccgagcgc cgcgatcccc gtcgccgaga
      181 cgtgattcag gtggtccgcg ctcctggcgg cgagcgcgac ggcggcctcg gccgcgcccg
      241 agggtccgag ctggtcggcg tggcagcgca gcgggagccc gacgtccgag gcgcaccgtg
      301 cgacctccgc gaggtcgtcg acggagaagg cgatgtcctc cacgtacaca tcgaccgcgt
      361 cgacgagacg ctcgcgcgcg gccgcaggga tcagctcgtc gcagacgacg cggacccagt
      421 cggcgcgctc catgccttcg ggcacggcgt ggcacgcgag cagggtgacc gaacacgtct
      481 gcggcgcctc ctcggacagc cgccgggcga ggcgggcctg tcggagctcg gcctcgaccg
      541 agagcccgta cccggtcttg agctcgagcg ccgtggtccc gtgctcggcc atctcctgca
      601 cgagcgaccg gcagaaggcg agcacctcgt cgtcgctcgc cgccgcgagc atgcgcgcgc
      661 tccggtagat cccgcctttc cccgggggcc taccgctcgc gccttcgcgc tcgctccatg
      721 agccccccgc ccccctgccg tgcaggtcgc ggtaggagac cccggctaaa cgcgcctcga
      781 actcgtccgc ccgccatccg gcgaacggca ggtgcgtgtg gcagtcgacg aacccaggaa
      841 cgatcgtgca gccctcgaac cactcgggct cgacgcccgc cagaccgtcg gcggggccga
      901 cgtaggtgat gaggccgtcc tcccacgcga ccgcctgctc gacggattcc tccggccacg
      961 gcccctcgcc gcggacctgc gcgaggtgcg ccgacggcgt ggcgaggctt gtcgcggcga
     1021 tcgcgccgct cgcgtggtcg ggcacgccga catgaaagag cagggtcggg tcgcacgcaa
     1081 gcttccgcat cggtcgcgcg ttcgcgctcg ttctcagccg gcgaatcccc gtccgtagac
     1141 tgtgccgcca tgagcgaggc ggagcggttc cggatccgag aggtgctcgc catccccgag
     1201 atccgagccg cgatgctcgg gaccttcgtg atcatgctcg ggtacggcat catctcgccg
     1261 gtcctgccga actacgcccg cagcttcggc gtcgggtacg actcgatcgg cctgctgatc
     1321 tcgtcgttct cgttcgcgcg cctggtgtcg gacccgttcg tcggccgcta catcgaccgg
     1381 tacggggagc gcgggatggc gacgctcggc gcggcgtggg tcggggtctc gtcgttcgcg
     1441 gcggcgctcg cgcccacgtt cccgttgctc gtgctcttcc gagcgctcgg gggggtgggc
     1501 tcggcgctgt tcttcgcctc gctgctctcc ttcctcttgc gatcgatccc gtcgcaccgg
     1561 acgggacgcg tgatgagcgt gtactacgcg tcgttcaaca tcggcttcat cgccggcggt
     1621 ccgctgggcg gcctgatcgc gaagtggttc accctcgcga caccgctcta cgtctacgcg
     1681 ggcgcatgct tcctcgcggc cgcgctgttc tggcggacga tccgcgaccc cgaacgtcac
     1741 gagtccgagg tccgtcgcgg cggcatccgc cggctcccgt ggaaccgggc gttcgtgacc
     1801 gtgctcgtcg tgaacggcgt ctacctctgg atgatcggcg ccgtcttcag caccttggtc
     1861 ccgctgttcg ggacgagcca cgaggtcggg ctcacgatcg gcggcgtggg gatcgggctc
     1921 gcgatcgcca cggcgaccga gctcgcgtgc ctgtaccccg ccggtcacgc gaccgaccgc
     1981 cgcggccggc gcgccgtcct gatccccgcg ctcgcggggc tcgcgctgat cacggcgggg
     2041 ttcgggttcg tctcgacgcc cgtcgggttc atgaccgcga tggcgctcct cggcgtcgcg
     2101 agcggctact cgggtgtccc gccggcgccg atgctgtcgg acgtgacgcc ggaggagctc
     2161 aaggggtcgg cggtcgccgt cttccggttc gtgggcgacg tcgggttcgt gatcgggccg
     2221 ctggtcgccg ggtggtcggc gcaggcgtac gggttcaccg catcgttcgc gatcaacgcg
     2281 atcccagcgg tcgtcgcgct ggggctcgtg ctctcgatcc gcgagacgat gcacgcgcgg
     2341 cgcgcgagcg acgagagcgg gggcctgtag cgatgcgggt ccaggcgttc cgggacctcg
     2401 ccgcgtcccc cgagcgggtc tggtcgtcga tcgaacggtg ggaggagcag tcgcggtgga
     2461 tccgcgacgc ggcctgggtt cgcgtgctca cgccggaacg cacgggcgtc ggcgcccgca
     2521 tcgaggtcct gagccgcgtg ctgcacgtcc cgctgttcac cgagcagctc gaggtcgtcg
     2581 gatgggagcc gccgcgaacg gtggtgatgg cgcataggag cttcgtccgc ggcaccggga
     2641 cctggtcgct cgaacgcgtg gacggctcga ccaggttcag ctggaccgag gagctctcgc
     2701 tgccgatccc ggtcctgggc gagctggccc tgctcgtcta ccggccgttc ctgcgccgcc
     2761 tcatgcgccg cagcctcgcg aggcttcagc gcctggtcgc gtcgacgtag gcgcgcaggt
     2821 ccggcgggtg cgcgggcggt agcattcgaa cagagtgcag gggagtccgg ggaaaccggg
     2881 ctgagaggcc ggctcgggtt cgggcacgcg aagcgcgcgg accgccgccg gcgacccttg
     2941 gaacctgatc cgggtcacac cggcggaggg agcggacgtg tcgcgcgtcg ccgaacgtct
     3001 cgagaagatc ctccggcgcg agccacccgc gtggggcatc cgccccgtgc cgctcgagcg
     3061 gcgcaacctc ggcggtctcg atctcgccgt cctgtggttc gacctgtcgg tggggcttct
     3121 cgtgatggtc accggggcgc tcctggtccc ggcgctcggg accgggcgcg cattcctcgc
     3181 gatcggggtg ggcacgctga tcgggtgcgt cccgctcgcc ctggtcgggc tcgccggcga
     3241 gcgcgagggc gtccccacga tggtcctgct ccgcccggta ttgggcgcgc gaggctcgtt
     3301 c
//
LOCUS       DATBRK010000359         9326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_502814, whole genome shotgun sequence.
ACCESSION   DATBRK010000359 DATBRK010000000
VERSION     DATBRK010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502814"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VIH70_10660"
     CDS             <1..1134
                     /locus_tag="VIH70_10660"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY8116864.1"
                     /translation="QQGLLLQPRTLEGPTGARARFDGRDVINLASNNYLGLASHPRMN
                     AAASKAASELGAGTGAVRTIAGSMSLHRELELRFAAFKHAEDALMFQSGFTANAGTVA
                     AILDKQDVIVSDRLNHASIIDGARLSRAEIKVFQHKDADHADALLRESKAEGRRQLLV
                     TDGVFSMDGDIAPLPALVEVAERHGAIMMIDDAHGSGVLGRGGAGTVDHFGLHGRVDV
                     QVGTLSKAVGVLGGFIAGPHELIEWLQNRGRPYLFSTSAPPAAVAACIEALDIIKDEP
                     ERLDRMWSNTRSFKAGLHELGFDTGASETPITPVITGDEERTQAFARRLFEEGVFCPA
                     IVFPTVAKGSARVRTIVTADHTEKDLGEALEAFGRVGEELGLR"
     gene            complement(1145..1831)
                     /locus_tag="VIH70_10665"
     CDS             complement(1145..1831)
                     /locus_tag="VIH70_10665"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8116865.1"
                     /translation="MNVRTKTEVSEETRHREPLTRDRIIDAALRVMDAEGLEAVTMRR
                     IGRELGVEAMSLYNHVQDKDDILEGVTERVMNEFEFPGSTDDWADDARAMSHEWRRLL
                     GLHPSVCQLLAERHKPLDGLATFRAMDSALGVLRRAGLSARDAAQAFNALGGYILGFV
                     MMEQGLMLGNDDEHEREHDLAVDALQSSGLLNVMECFPFFADCTTDQQFDFGLDLMIR
                     GIRAGVEGIA"
     gene            1997..4330
                     /locus_tag="VIH70_10670"
     CDS             1997..4330
                     /locus_tag="VIH70_10670"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY8116866.1"
                     /translation="MRLNPESIARASSRHPWRTVGIWAVVLILGFGASGALLSDALTT
                     DFDFTNNPEAIRAQKLLEQKQLEQDVSPETFVLVGGDGATTDPQFASTVNSALDDLRA
                     LDPSVVLQVPTGYPLSEQDASNPQVAALGPIDSKDGTAVLFTAILTGDSDTTNAHVEE
                     LNTIRERYSTDGTKMYALGEPTSTDDFKKISEEDLQKGEGIGVLVAIIVLLVVFGSLL
                     AGVTPIIMGIFAIGIAFGLIGLLGQVWRFSFFVPNLISMMGLAVGIDYSLFIVSRYRE
                     ERSRGRDNLEAIGRSGATANRAVFFSGVTVVLALAGMLLVPTTIFRGLAGGAILVVLI
                     SLALSMTLLPALMALFADHLVKPGWLFGRGRTLEHGRPGGFWDTVTRAVMARPWAWLL
                     VAASFMLLLSVPYWAQSNPKDDARGMREGFSGITTIPQGIQTRDAFDVLIAKFPTTGT
                     QGSTADVVIPGDGNDPTVQTGIQTLEDAATADPSFGEPLAPQTSADGTVTLVRFPFAG
                     EATDNSSEAAVDAITQMRETYVPQAFGQDSGVLVGGDTATAVDFFDVTAFYTPIIILL
                     VLGLSFILLTVVFRSIVVPAKAILMNLLSVGAAYGLIVLIFQKGGPSFAHSIANALNF
                     IQVDAIEAWLPLFLFSILFGLSMDYQVFLITRIREEYDKTGDNSEAVAFGLRTTGGII
                     TGAAIIMVAVFAAFASGRIAGIQQMGFGLAVAVFLDATIVRSILVPSAMKLLGHRNWY
                     LPGWLQWLPKLNVEGAVEEQQPVLLPQAEPVGVRDDA"
     gene            4401..5336
                     /locus_tag="VIH70_10675"
     CDS             4401..5336
                     /locus_tag="VIH70_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY8116867.1"
                     /translation="MEEAHGLELRGERDALREALGRIGAWSFALEAMPAPEERDAAAE
                     IESSGYRAIWIPEAVDSREAFAHAGWLLANTERAIVATGIANIWARDATAMANGWRML
                     SDAYPARFLLGIGVSHQGSVARRGALYERPLSAMREYLDAMDRAPSTAPEPEFAPRLV
                     LAALGPRMLELAAEHAHGVHPYFVPVEHTAFARQRLGPGPVLAVEQTVVLESDPSQAR
                     NVARGFALDYLQTENYARNLRRMGWTDADLRGQGSDALIDAVVAWGDVDRIAVRVRQH
                     LDAGADHVCVQVVGEDELDPCLPALRELAPALLEL"
     gene            complement(5338..6003)
                     /locus_tag="VIH70_10680"
     CDS             complement(5338..6003)
                     /locus_tag="VIH70_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEY8116868.1"
                     /translation="MLRTLEEMGDPSRLEGMARYGIRTDRAVGVPVTELRRLARGLYP
                     DHELAAALWTSGPHEARILASLVDDPALVSEAQMDAWVSDLDSWDVCDAVCGNLFDRT
                     PFAMNKAVEWTGREPEFERRAGFVVMACAAVHRKDLPDRAFAALLPTIRVASTDDRTY
                     VKKAVSWALRQIGKRSLGLNAKAIRTAEQIGRIDARPSRWVARDALRELRSDAVHARL
                     LRR"
     gene            complement(6057..6332)
                     /locus_tag="VIH70_10685"
     CDS             complement(6057..6332)
                     /locus_tag="VIH70_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116869.1"
                     /translation="MGVFILILLLLAAMAGVLGAVLKATLVIVLSLVLAVVLLAWIGA
                     WYARRRMRAFQRDFQIRVDRSRRRSEAYDIARQPPADPPAGRLGDGR"
     gene            complement(6367..7236)
                     /locus_tag="VIH70_10690"
     CDS             complement(6367..7236)
                     /locus_tag="VIH70_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8116870.1"
                     /translation="MPNVRDAARFQEVRTDGVRLNVRRWPGGPNEALLIHGLASSSHI
                     WDLVAPRLGRLGIEPVAYDQRGHGRSSKPSSGYGFGRTAADAAAVIRATGLRRPIVVG
                     HSWGANVALELAMRHPRLVSGAILLDGGFSSMRDRFDWRTAKAVLSPPAFGGIPVEAF
                     VAGIPEHTGLARTPEIETVVLSLVRVDGLGRVHPRLARANHLRILHALWAQDTDGLLR
                     RVRVPTLVMAARTRERAPDQAGFARAKVEAARRVRAIGPPVAFEWIEGIHDLPLQRPE
                     AVARRIARFAGDA"
     gene            7332..7601
                     /locus_tag="VIH70_10695"
     CDS             7332..7601
                     /locus_tag="VIH70_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116871.1"
                     /translation="MTPKAGRRDELVAMMSSPPTGAAANGYRGGYVLKADEGDDVVVA
                     VMYDDKDAYVAMVHDPQTEANFGKIMELLEAEPSWTDGEWIGPEA"
     gene            7691..9139
                     /locus_tag="VIH70_10700"
     CDS             7691..9139
                     /locus_tag="VIH70_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8116872.1"
                     /translation="MADALQNFIDGKWVDTRDGERFDVFDPATGEVIATAPDSKAVDV
                     EAAIDAARRTFDDGTWWPGTPARERGRILLRAADIVRRERERLAGMESLDSGKPIGDA
                     REDIDEIAFMFEYFGGWATKIDGDVQHLSPEAMFMVWKEPMGVAVGITPWNYPMMMAT
                     QKLAPALAAGCTFVLKPPEQTPLTCLELPKILEEAGLPAGVFHVLTGFGETAGAPLVA
                     SPKVDKVAFTGSREVGKIIMRSGADTLKRVTLELGGKSPNIVFGDADRATAFEGSANG
                     VFWNQGEICSAGTRVFVERPLYDDAVNAFVDRASSIVLGEGSDEATEMGPLVSKEQQE
                     RVERFIEIGSKEATLAKQGSRPSDARLAGGYFVPPTVFADVDNDATIAREEIFGPVMS
                     VIPFASVDEVVAMSNDNEYGLAAAVWTNDVKKAMLTARALRAGVVWINDSQPAPTEMP
                     WGGYKQSGIGRELGKHGVEDFLESKSVYLNLG"
BASE COUNT         1435 a         3179 c         3298 g         1414 t
ORIGIN      
        1 cagcagggac tcctgctgca gccgaggacc ctcgagggac ccacgggcgc ccgggctcgg
       61 ttcgacgggc gggacgtgat caacctcgcc tcgaacaact acctcgggct ggcgagccac
      121 ccccggatga acgccgcggc ctccaaggcc gcgagcgagc tcggcgccgg aacgggtgcc
      181 gtgcggacga tcgcgggatc gatgagcctg caccgcgagc tcgagcttcg gttcgccgcc
      241 ttcaagcacg ccgaggacgc tctgatgttc cagtcgggct tcacggcgaa cgccgggacc
      301 gtcgccgcca tcctggacaa gcaggacgtg atcgtgagcg accgcctcaa ccacgcgtcg
      361 atcatcgacg gcgcgaggct ctcgcgcgcc gagatcaagg tgttccagca caaggatgcc
      421 gaccacgcgg acgccctgct cagggagagc aaggccgagg gtcggcgaca gctgctggtg
      481 acggacggcg tgttctcgat ggacggcgac atcgcgcccc tgcccgcgct cgtcgaggtc
      541 gccgagcgcc acggggcgat catgatgatc gacgatgcgc acgggtcggg cgtcctcggc
      601 aggggcggcg ccgggacggt cgaccacttc ggtctccacg gccgggtcga cgtgcaggtc
      661 ggcacgctct cgaaggcggt cggcgtgctg ggtggcttca tcgccgggcc gcacgagctg
      721 atcgagtggc tgcagaaccg cggccggccc tacctgttct cgacgagcgc accaccggcg
      781 gcggtcgcgg cgtgtatcga ggcgctcgac atcatcaagg acgagcccga gcggctcgat
      841 cgcatgtggt cgaacacgcg atcgttcaag gccgggctgc acgagctcgg gttcgacacc
      901 ggtgcgagcg agacgccgat caccccggtg atcaccgggg acgaggagcg gacgcaagcg
      961 ttcgcgcggc ggttgttcga ggagggggtg ttctgccccg cgatcgtgtt ccccacggtc
     1021 gcgaagggct cggcccgcgt gcggacgatc gtgaccgccg accacacgga gaaggacctg
     1081 ggcgaggccc tcgaggcgtt cggtcgggtc ggcgaggagc ttgggcttcg ttagcgggac
     1141 ggccctaggc gatgccttcg accccggccc ggatcccgcg gatcatcaga tccagcccga
     1201 agtcgaactg ctggtcggtc gtgcagtccg cgaagaacgg gaagcactcc atcacgttga
     1261 ggagcccgga gctctgcagc gcgtcgacgg cgagatcgtg ctcccgctca tgctcgtcgt
     1321 cgttgccgag catcagcccc tgctccatca tcacgaagcc caggatgtag ccgcccagcg
     1381 cgttgaaggc ctgcgcggcg tcgcgggccg acaggccggc gcgacgcagc accccgagcg
     1441 cggagtccat cgcccggaac gtcgcgagac cgtcgagtgg cttgtggcgc tccgcaagca
     1501 gctggcagac gctcggatgt aggccgagga gccgccgcca ctcgtgagac atcgctcgcg
     1561 cgtcgtcggc ccagtcgtcg gtggatccgg ggaactcgaa ctcgttcatc acgcgttcgg
     1621 tgacgccctc gaggatgtcg tccttgtcct ggacgtggtt gtacagcgac atcgcctcga
     1681 cgccgagctc ccgcccgatc ctgcgcatcg tgacggcctc gagaccctcc gcgtccatga
     1741 cgcgcagcgc cgcatcgatg atccggtctc gggtgagcgg ctcgcggtgc cgcgtctcct
     1801 cgctgacctc ggtcttcgtc cgcacgttca tccaccgtcc atactcgtca gagaaacttt
     1861 ccgatccggg aagcgacgac cggttcccgg ggttgacacc ttacatcgta cgcctatact
     1921 ccgtacgttg taagcatatg gtgtaagtcg gaggaaggca agattccgac actcggcagg
     1981 aagcgaggga gacaggatga ggctcaaccc ggagtcgatc gcacgcgcga gcagccgtca
     2041 cccgtggcgg accgttggga tctgggcggt ggtcctgatc ctcgggttcg gagcgtccgg
     2101 ggccttgttg agcgacgcgc tcaccacgga cttcgatttc acgaacaatc ccgaggcgat
     2161 ccgggcgcag aagctcctcg agcagaagca gctcgagcag gatgtctcgc cggagacctt
     2221 cgttctggtc ggaggcgacg gcgccacgac cgacccgcag ttcgccagca cggtgaactc
     2281 agcccttgac gatctgcgag cgctcgatcc gtcggtggtg cttcaggtcc cgaccggcta
     2341 cccgttgtcc gaacaagacg cgtcgaaccc gcaggtcgcc gccctcggac cgatcgattc
     2401 gaaggacggc acggcagtgc tgttcacggc gatcctcacc ggggactcgg atacgacgaa
     2461 cgcacacgtc gaggaactga acacgatccg cgagcggtac agcaccgatg gcaccaagat
     2521 gtatgcgttg ggcgagccga cctcgaccga cgacttcaag aagatctccg aggaggacct
     2581 ccagaagggc gaggggatcg gagtcctcgt cgcgatcatc gtcctgctgg tcgtgttcgg
     2641 ctcgctcctg gccggcgtga ccccgatcat catggggatc ttcgcgatcg gcatcgcgtt
     2701 cgggctgatc ggcctgctcg ggcaggtgtg gaggttcagc ttcttcgtgc cgaaccttat
     2761 ctcgatgatg ggtcttgcgg tgggcatcga ttactcgttg ttcatcgtgt cccggtaccg
     2821 ggaggaacgg tcgcgggggc gtgacaacct cgaggccatc ggacgctccg gggcgacggc
     2881 gaaccgggcc gtgttcttca gcggggtcac ggtcgtcctg gcgcttgcgg gcatgctcct
     2941 cgtgccgacc acgatcttcc gcgggctggc cggcggcgcg atcctggtcg tgctgatctc
     3001 gctcgcgctg tcgatgacgt tgcttcccgc gctgatggcc ctgttcgcgg accacctggt
     3061 gaagcccggg tggctcttcg gtcgcggtcg cacgctggag cacggtcggc ccggtggatt
     3121 ctgggacacc gtgacgcgcg ccgtgatggc tcggccgtgg gcgtggctgc tcgtggccgc
     3181 cagcttcatg ctgctgctgt cggtcccgta ctgggcacag tcgaacccga aggacgacgc
     3241 tcgcggcatg cgcgaggggt tctcgggcat caccacgatc ccgcaaggga tccagacgag
     3301 ggatgccttc gacgtcctca tcgcgaagtt ccccacgacg ggcacgcagg gttccaccgc
     3361 cgacgtcgtg atccccggcg atgggaacga tccgaccgtc caaacgggga tccagacgtt
     3421 ggaagacgcc gccaccgctg acccgtcgtt cggtgagccg ctggcgccgc agacgagcgc
     3481 ggacgggacc gtcacgctcg tgcgcttccc gttcgccggg gaggcgaccg acaactcgag
     3541 cgaggcggcg gtcgacgcca tcactcagat gcgagagacg tacgtgccgc aggcgttcgg
     3601 ccaggactcc ggcgtcctcg tgggtggcga taccgcaacg gcggttgact tcttcgacgt
     3661 caccgcgttc tacactccga tcatcatcct gctggtgctc gggctgtcgt tcatcctgct
     3721 caccgtcgtg ttccgatcga tcgtcgtgcc ggccaaggcg atcctgatga acctgctctc
     3781 ggtgggcgct gcatatggcc tcatcgtctt gatcttccag aagggtgggc cgtcgttcgc
     3841 tcactcgatc gcgaacgcct tgaacttcat ccaggtcgac gcgatcgagg catggctccc
     3901 gctgttcctg ttctcgatcc tgttcggcct ctcgatggac taccaggtct tcctgatcac
     3961 ccggatccgg gaggagtacg acaagaccgg ggacaactcc gaggcggtcg ccttcggtct
     4021 gcggaccacg ggcgggatca tcacgggtgc cgcgatcatc atggtggccg tgttcgccgc
     4081 cttcgccagc gggcggatcg ccggcatcca gcagatgggg ttcggcctcg cggtcgccgt
     4141 cttcctcgac gcgacgatcg tgcgatcgat cctcgtcccg tcggcgatga agctgctcgg
     4201 ccaccgcaac tggtacctgc ctgggtggct gcagtggctg ccgaagctca acgtcgaggg
     4261 ggccgtggaa gagcagcagc ccgtgctgct ccctcaggcc gagcccgtcg gcgtgaggga
     4321 cgacgcctga tcttcccagc gtcgccaagg aaggggcccc ccatgcgggg gccccttcct
     4381 cgttgatagg gtccggcgcc atggaggaag cccacggcct cgaacttcgc ggtgagcgcg
     4441 acgccctccg ggaggcgctc gggcggatcg gggcgtggag cttcgcgctg gaggcgatgc
     4501 cggcgcccga ggagcgggac gccgcggccg agatcgagtc gtccggctac cgagcgatct
     4561 ggatcccgga ggcggtggac agccgcgagg cgttcgccca cgcgggctgg ctcctggcga
     4621 acaccgagcg cgcgatcgtc gccaccggga tcgcgaacat ctgggctcga gacgcgaccg
     4681 cgatggccaa cggctggcgc atgctctcgg acgcgtaccc ggcgaggttc ctcctcggca
     4741 tcggggtgag ccaccagggc tcggtcgcgc gccgcggggc cttgtacgag cgccctctct
     4801 cggccatgcg tgagtacctg gacgcgatgg accgggcccc gtccacggcg cccgaacccg
     4861 agttcgcccc gcgtctggtg ctggcggcgc tcgggccgcg gatgctcgag ctcgcggccg
     4921 agcacgcgca cggcgtgcat ccgtacttcg tgccggtcga gcacacggcg ttcgcgcggc
     4981 agcggctcgg ccccggcccg gtgctggcgg tcgagcagac ggtggtgctc gagagcgatc
     5041 cgtcgcaggc tcgaaacgtc gcccgcgggt tcgccctgga ctacctgcag acagagaact
     5101 acgcgcggaa cctccggcgc atgggctgga cggacgccga cctgcgcggc caggggagcg
     5161 acgcgctgat cgacgcggtg gtggcgtggg gcgacgttga tcggatcgcg gtgcgcgtcc
     5221 gccagcacct ggacgccggg gccgaccatg tctgcgtcca ggtggtgggc gaggacgagc
     5281 tcgacccgtg cctgccggcg ctccgcgagc tcgcgcccgc gttgctcgag ctctaggtca
     5341 gcgccgcaga agccgcgcat gcacggcgtc gctgcgcagc tcgcggagcg catcgcgagc
     5401 gacccaccga gacgggcggg catcgatccg cccgatctgc tccgcggtcc ggatcgcctt
     5461 ggcgttcagc ccgaggctcc gcttgccgat ctgccgcaac gcccacgaga ccgccttctt
     5521 cacgtacgtg cggtcgtccg tggacgccac gcggatcgtg ggcaggagcg ccgcgaacgc
     5581 ccggtcgggg aggtccttgc ggtggaccgc ggcgcacgcc atcaccacga acccggcccg
     5641 ccgctcgaac tccggctcgc gcccggtcca ctccaccgcc ttgttcatcg cgaacggcgt
     5701 gcgatcgaac aggttgccgc acactgcgtc gcacacgtcc cacgagtcga ggtccgagac
     5761 ccaggcgtcc atctgcgctt cggacacgag ggcggggtcg tcgaccagcg acgcgaggat
     5821 gcgcgcctca tgggggcccg acgtccacag ggcggccgcg agctcgtggt cggggtacag
     5881 gccgcgcgcg agacgccgga gctcggtgac ggggacgccg accgcgcggt cggtccggat
     5941 cccgtagcgc gccatcccct ccaggcggga cgggtcgccc atctcctcga gcgttcgaag
     6001 cacgtcgcgg gggtttcgga cgctcttcga tgccgacggg atcgtcgcgc cccgcgctac
     6061 cggccgtcgc ccagccgccc cgcgggggga tcggcgggcg gctggcgggc gatgtcgtag
     6121 gcctcgctcc ggcgacggga ccgatcgacc cggatctgga agtcgcgctg gaacgcgcgc
     6181 atccgccgcc tcgcgtacca ggcgccgatc caggccagca gcacgacggc cagcaccagc
     6241 gagaggacga tcaccagggt ggccttcagg acggcgccca gcacgccggc catcgccgcg
     6301 agcagcagca ggatcaggat gaagaccccc atgacctccc atcctacgag cgccgcgatc
     6361 gaacgttcag gcgtcgccgg cgaaccgggc gatgcgccgg gccacggcct ccggccgctg
     6421 gagcggcagg tcgtggatcc cctcgatcca ctcgaacgcc accggcgggc cgatcgcccg
     6481 gacgcgacgc gccgcctcca ccttcgcgcg ggcgaacccg gcctggtcgg gggccctctc
     6541 gcgcgttcgc gcagccatca cgagcgtcgg gacacggacc cggcgcagca gcccgtccgt
     6601 gtcctgcgcc cacagggcgt ggaggatcct caggtggttc gcgcgcgcga ggcgcgggtg
     6661 cacgcgcccg aggccatcca ctcggaccag cgacaggacg accgtctcga tctccggtgt
     6721 tcgcgccagg ccggtgtgct cggggatccc ggccacgaac gcctcgaccg ggatcccgcc
     6781 gaaggcgggc ggcgacagca ccgccttggc ggtccgccag tcgaaccggt cccgcatcga
     6841 ggagaagccg ccgtccagca ggatcgcccc ggacaccaga cgagggtggc gcatggcgag
     6901 ctcgagggcg acgttcgcgc cccatgagtg accgaccacg atcgggcgcc gcaggcccgt
     6961 ggcgcggatg accgcggccg cgtcggcggc cgtgcgcccg aacccgtaac ccgacgacgg
     7021 cttgctcgaa cgcccgtgcc cgcgctggtc gtacgccacc ggctcgatcc cgaggcggcc
     7081 gagcctcggc gcgaccagat cccagatgtg cgacgaggac gcgaggccgt ggatcagcag
     7141 ggcctcgttc gggccgcccg gccaccgccg gacgttcagt cggacgccgt cggtccgaac
     7201 ctcctggaaa cgtgcggcgt cccgtacgtt cggcacgcgg gtgagcctac cggccaccga
     7261 acccgccgcc gtatactccg ccgcgttcga ggggcgaagg gagaccactc gtgtacggaa
     7321 cactcggacg gatgacaccg aaggccggca ggcgcgacga gctcgtggcg atgatgagca
     7381 gccccccgac cggcgcggcc gcgaacggct accgaggcgg ctacgtcctc aaggccgacg
     7441 aaggcgacga cgtcgtggtg gccgtgatgt acgacgacaa ggacgcctac gtcgcgatgg
     7501 tccacgatcc ccagaccgag gcgaacttcg gcaagatcat ggagctgctt gaggccgagc
     7561 cgagttggac cgacggcgag tggatcggac ccgaggcctg accaccgggg ttcccaaccc
     7621 cggtcacgcc tccgagcggc gcgcgatagg ctgaacgctc gttcaggaaa acctccggga
     7681 ggcgagcgcc gtggccgacg cgttgcagaa cttcatcgat gggaagtggg tcgacactcg
     7741 cgacggcgag cgcttcgacg tcttcgaccc cgcgaccggc gaggtgatcg cgaccgcccc
     7801 ggactcgaag gccgtcgacg tggaggctgc gatcgatgcc gcccggcgga cgttcgacga
     7861 cggcacgtgg tggcccggca cgcccgcccg cgagcggggt cggatcctgc tccgcgccgc
     7921 cgacatcgtg cggcgcgaac gcgagcgcct ggccgggatg gagtcgctcg actcgggcaa
     7981 gccgatcggc gacgcgcgcg aggacatcga cgagatcgcg ttcatgttcg agtacttcgg
     8041 ggggtgggcg acgaagatcg acggcgacgt ccagcacctg tccccggagg cgatgttcat
     8101 ggtgtggaag gagccgatgg gggtcgccgt cggcatcacc ccatggaact accccatgat
     8161 gatggcgacc cagaagctcg cccctgcgct cgcggccggc tgcacgttcg tcctcaagcc
     8221 tcccgagcag accccgctca cctgcctcga gctgccgaag atcctcgagg aggccgggtt
     8281 gcccgcggga gtgttccatg tcctcaccgg gttcggcgag actgccggtg cgccgctcgt
     8341 cgcgagcccg aaggtggaca aggtggcgtt caccggttcc cgcgaggtcg gcaagatcat
     8401 catgcgcagc ggagccgaca cgctgaagcg cgtgaccctc gagctggggg gcaagtcgcc
     8461 caacatcgtg ttcggggacg cggatcgcgc gaccgccttc gagggctcgg cgaacggcgt
     8521 gttctggaac cagggcgaga tctgttcggc gggcacgcgc gtgttcgtcg agcggccgct
     8581 gtacgacgat gcggtgaacg cgttcgtcga ccgggcctcg tcgatcgtgc tgggtgaggg
     8641 gtccgacgag gcgaccgaga tgggtccgct cgtgagcaag gagcagcagg agcgagtcga
     8701 gcggttcatc gagatcgggt cgaaggaggc gacgctcgcc aaacagggtt cgcgcccgtc
     8761 cgacgcgcgg ttggcgggcg gctacttcgt cccgccgacc gtgttcgccg acgtcgacaa
     8821 cgacgcgacg atcgcgcgcg aggagatctt cggtccggtg atgtcggtga tccccttcgc
     8881 ctcggtcgac gaggtcgtgg cgatgtccaa cgacaacgag tacgggctcg cagcggccgt
     8941 gtggaccaac gacgtgaaga aggcgatgct cacggcccgg gcgctccgcg ccggcgtggt
     9001 gtggatcaac gactcgcagc cggcgcccac cgagatgccg tggggcggct acaagcagtc
     9061 ggggatcggt cgcgagctcg gcaagcacgg cgtcgaggac ttcctcgaga gcaagtcggt
     9121 ctacctcaac ctcggctgac gtagggaccc tcgagccgga cggctcctcc ggcggtcgcc
     9181 ggggccctcg ggtatgctcc gcggtcatgg ccggtggaga ggtcgtcctc gtcgatctgg
     9241 tgaagcggtt cgccgacgtg acggcggtcg cggggatcaa cctgtcgatg ccctcgggcg
     9301 agttcttctc gctgctcggc ccctcc
//
LOCUS       DATBRK010000360         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2089052, whole genome shotgun sequence.
ACCESSION   DATBRK010000360 DATBRK010000000
VERSION     DATBRK010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089052"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VIH70_10705"
     CDS             <1..1182
                     /locus_tag="VIH70_10705"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8116873.1"
                     /translation="LDQGKPMRAEAFDEVEELIAYFEMAAADAVRVEGLIPPSVTQGN
                     RVLVYRVPRGVAGVISPWNWPYTMPGEIVAPALASGNTVVLAPAPTTSVCAVALAEVI
                     AEADLPAGAFNLVTGPGPVVGDEVASNPGVSTVGFIGSIATGHRVAERAAGKELLLEM
                     GGNGPLVVLEGADLDAAVEATLTACFLNAGQSCTAGERVLVHERVHDEYVGRLTTAIA
                     ERIRLGDPFDDATTMGPLNNAPVADKTEKHVSEAAELGAAIVTGGKRAPQFGSDLFFE
                     ATVVDGVTDEMEIAREETFGPVVPVQTIRHEDDAIALVDRSEYGLLSAIFTADIGRGL
                     RFAERVRTGWVNVNEGTNYWESHLPFGGRAGSASGVGRVGGRFSIERLTELKTIVVHL
                     G"
     gene            1192..>1805
                     /locus_tag="VIH70_10710"
     CDS             1192..>1805
                     /locus_tag="VIH70_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8116874.1"
                     /translation="MVRSKGRPTRLFFAADLHGSQPTFRKFLGAAAFYETDALVFGGD
                     LMGKALVPIVRSNGVFRAHFQGRDHEFDEDGLAEFTRSVELPGFYWHVFEPDEYEAVK
                     DDELAQLGWFHRLASERLAEWISLAEQRLAGTGVRMYLTGGNDDEPAMLEVLDRHDGP
                     NVIACEDRVVELDDEHTMITVGLSTVTPWDTPREASEEVIAAAI"
BASE COUNT          272 a          607 c          672 g          254 t
ORIGIN      
        1 ctcgaccagg gcaagccgat gcgggcggag gcgttcgacg aggtcgagga gctgatcgcg
       61 tacttcgaga tggcggcggc cgacgccgtc cgggtcgagg gcctcatccc gccctcggtg
      121 acccagggca accgtgtgct cgtctaccgg gtgccgcgcg gagttgccgg cgtgatcagc
      181 ccgtggaact ggccctacac gatgccgggc gagatcgtcg cgcccgccct cgcgagcggg
      241 aacacggtgg tgctcgcgcc ggcgcccacc acgagcgtgt gcgcggtggc gctcgcggag
      301 gtgatcgccg aggccgacct acccgccgga gcgttcaatc tggtgacggg ccccggcccc
      361 gtggtcggcg acgaggtggc ctcgaacccg ggcgtctcga cggtcgggtt catcggctcg
      421 atcgccaccg gccaccgcgt ggccgaacgc gccgcgggca aggagctgct gctcgagatg
      481 ggcggcaacg ggccgctggt cgtgctcgag ggcgccgacc tcgacgccgc ggtcgaggcc
      541 acgctcacgg cctgcttcct gaacgccggt cagagctgca ccgccggtga acgggtcctg
      601 gtgcacgaac gcgtgcacga cgagtacgtc ggcaggctca cgaccgcgat cgccgagcgc
      661 atccggcttg gcgatccgtt cgacgacgcc accacgatgg gaccgctcaa caacgcgccc
      721 gtggccgaca agaccgagaa gcacgtgagc gaggccgccg agctcggcgc cgcgatcgtg
      781 accggcggca agcgcgcgcc gcagttcggc agcgacctgt tcttcgaggc cacggtcgtc
      841 gacggcgtga ccgacgagat ggagatcgcg cgcgaggaga cgttcgggcc cgtcgtgccg
      901 gtccagacga tccgccacga ggacgacgcg atcgcgctcg tcgaccgctc cgagtacggg
      961 ctgctctcgg cgatcttcac ggccgatatc ggacgcggcc tgcggttcgc cgagcgcgtc
     1021 cggaccggct gggtcaacgt gaacgagggc acgaactact gggagtccca cctgccgttc
     1081 ggtgggcggg cgggttctgc gagcggggtc gggcgggtcg gcggccgctt ctcgatcgaa
     1141 cggctgaccg agctcaagac gatcgtggtg cacctgggat aggaggccgg cgtggtccgg
     1201 tcaaaggggc ggccgaccag actcttcttc gcggcggacc tgcacgggtc gcagcccacg
     1261 ttccgcaagt tcctcggcgc ggccgcgttc tacgagaccg acgcgctggt gttcggcggc
     1321 gacctcatgg gcaaggcgct ggtgccgatc gtgcgctcga acggtgtgtt ccgggcgcac
     1381 ttccagggcc gcgatcacga gttcgacgag gacggcctgg ccgaattcac gaggagcgtc
     1441 gagctgcccg gcttctactg gcacgtgttc gagcccgacg agtacgaggc cgtcaaggac
     1501 gacgagctcg cgcagctcgg gtggttccac cgcctggcgt ccgaacggct ggccgagtgg
     1561 atctcgctcg ccgagcagcg tctggccggc accggcgtcc ggatgtacct gaccggtggc
     1621 aacgacgacg agccggcgat gctcgaggtc ctcgaccgcc acgacggccc gaacgtgatc
     1681 gcctgcgagg atcgcgtggt cgagctcgac gacgagcaca cgatgatcac ggtcggcctg
     1741 tccacggtga cgccgtggga cacgccgcgc gaggcgagcg aggaggtcat cgcggcggcg
     1801 atcga
//
LOCUS       DATBRK010000361         4382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_930132, whole genome shotgun sequence.
ACCESSION   DATBRK010000361 DATBRK010000000
VERSION     DATBRK010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930132"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VIH70_10715"
     CDS             <1..601
                     /locus_tag="VIH70_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEY8116875.1"
                     /translation="HLVVSGGLFHGIQLWVNLPKAEKWAPPRYQDLRATEVSLLSSPD
                     AGALVRVIAGEVAGHSGPGQTYTPMTMLHASLAPGAALELPWREDFNALAYVLAGAGS
                     AGVERVGVSTGQLVAFGPGDAIRLEASKGQESRSPELEVLLLGGRPIKEPVAWYGPFV
                     MNTKAELLQAFEDYQAGRLGTIPAAHHTPDHIVESGTDE"
     gene            718..1575
                     /locus_tag="VIH70_10720"
     CDS             718..1575
                     /locus_tag="VIH70_10720"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019746154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY8116876.1"
                     /translation="MSTSVRRTVAVISGLVLFVAGAATSAALRRNASSAERAPIQLPA
                     LDRAVFLSHVNDPARTPLFPGDPAFSIHTVFTVPDDGFYLELVREGAHTGTHYSAPCH
                     FHDGAVCMDDLSPSDLVLPAVVVDVRDEVSNDPDHVVRVAELRAWEDVFGEMPAGAAV
                     LLLTGCSDFWARGDRGGGANYFNCGSGRPGVHQPGFSRNAVRWLIRTGVLTETGALGT
                     DTFGPDPSSDAAFLPTWLTLRKHRLTIENLTNLDALPPTGAWVVLGSPRNANGSGAPG
                     TVFGLIPSG"
     gene            complement(1610..2524)
                     /locus_tag="VIH70_10725"
     CDS             complement(1610..2524)
                     /locus_tag="VIH70_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116877.1"
                     /translation="MADLLIGTREGLFALAGGEPRPEASFRAREVAALAVREGAAWAV
                     AGHNAIVRRAPDGTWTDVARSELQIACLADTSDGVLVGTEEAHLLRLFGDDGTLAPVE
                     AFETVEGRESWYTPWGGPPAVRSIAQDLAGRLHANVHVGGIPRSVDAGASWAPTIDID
                     ADVHQVIAHPTQPDVVLAAGAVGLAVSVDGGSSWRIEREGLHATYARAVAVAGDRVLV
                     SVSNGPRGGRGAVYRGALEPGATFERCTEGLPDWFDGNIDSGWLDARGSQVAFGTGDG
                     EVFVSQDAGERWTRAATDLPSIRAVRLL"
     gene            complement(2533..2994)
                     /locus_tag="VIH70_10730"
     CDS             complement(2533..2994)
                     /locus_tag="VIH70_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116878.1"
                     /translation="MDVRAIPEGMHTITPHIVVRDAARAAEWYASAFGAEERDRLPLP
                     GGKLMYVELWFGDSAVMVADEFPEAGVVSPQSIGGTPVVLHLFTDEVEALWERALGAG
                     AEVLHPLTDQFWGDRQGQLTDPFGHRWGLAQHVRDVPRDEIARAAASAFGG"
     gene            complement(3061..3408)
                     /locus_tag="VIH70_10735"
     CDS             complement(3061..3408)
                     /locus_tag="VIH70_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116879.1"
                     /translation="MSDWREQEVRNESRSRDRNEWIAETRGAVALDHETEELFVCECS
                     DPACRARITLTRIEYEAVRGYPTRFAIATDHENPEVDQLVSEGDRFTVVQKIAGAAAR
                     IARQTDHRHRDAE"
     gene            complement(3425..3709)
                     /locus_tag="VIH70_10740"
     CDS             complement(3425..3709)
                     /locus_tag="VIH70_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8116880.1"
                     /translation="MRVPSEDFSVLIEESNGALHLTLGGWLDAEAAPTLLAAVTRARP
                     RDVILEVHSLESIDGAGWLGVIACEHRVATWGGRLRIDNGIRKILDLASS"
     gene            3924..>4382
                     /locus_tag="VIH70_10745"
     CDS             3924..>4382
                     /locus_tag="VIH70_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116881.1"
                     /translation="MDEGGPERLRILVSDGQGHRLDEVLRSVTELGHEVVGQTPLGEV
                     AAASASVHPDAAIVIVGESTERALDLIGRIVTEAECPVIAILDVEDEGFIRKAAKRGI
                     FAYITLGRDASALQSSLDVVLSRFAEYQGLEGAFARRAITERAKGILMERH"
BASE COUNT          614 a         1554 c         1577 g          637 t
ORIGIN      
        1 gcacctggtg gtctcgggcg gcctgttcca cgggatccag ctctgggtca acctgccgaa
       61 ggccgagaag tgggcgccgc cgcggtacca ggacctgcgc gcgacggagg tctcgctgct
      121 ctcgtcgccg gacgcgggcg cgctcgtgcg ggtgatcgcc ggcgaggtgg ccggtcactc
      181 tggtccgggt cagacctaca caccgatgac gatgctgcac gcctcgctcg cgccgggcgc
      241 cgcgctcgaa ctcccctggc gcgaggactt caatgcgctg gcctacgtgc tcgcgggagc
      301 gggttccgcc ggcgtcgaga gggtcggcgt ctcgaccgga cagctggtgg cgttcggacc
      361 gggcgacgcg atccgcctgg aggcgtcgaa gggtcaggaa tcgcgctcgc cggagctcga
      421 ggtgctcctg ctcggcggcc gcccgatcaa ggagccggtc gcctggtacg gcccgttcgt
      481 gatgaacacg aaggccgagc tgctccaggc gttcgaggac taccaggcgg gcaggctcgg
      541 cacgatcccg gccgcgcacc acacacccga ccacatcgtc gagtccggca ccgacgagta
      601 ggcaccgccg gccgaccagg cgccacttcc ctccgctcgc gcccccgcgc catcggcgct
      661 agcgtccgct gggaccggca agcgtgggta tgtgccggcg ggcgaaggag ggacgcggtg
      721 tcgacatccg ttcggaggac cgtcgcggtg atctcggggc tcgtgctgtt cgtggctggg
      781 gcggccacgt ccgccgcgtt gcggcgaaac gcctcgagcg cggagcgcgc accgatccag
      841 ctccccgcgc tcgatcgcgc cgtgttccta agccacgtca acgaccctgc gaggacgccg
      901 ctgttcccgg gtgacccggc gttctcgatc cacactgtgt tcacggtccc cgacgacggc
      961 ttctacctcg agctggtgcg cgagggcgcc cacaccggga cgcactacag cgcgccgtgc
     1021 catttccacg acggggccgt gtgcatggac gacctctctc cctccgatct ggtgctcccg
     1081 gcggtcgtgg tggacgtccg cgacgaggtc tcgaacgacc cggatcacgt cgtgcgcgtg
     1141 gcggagctgc gggcgtggga ggacgtgttc ggtgagatgc cggcgggcgc ggccgtcctg
     1201 ctgctcaccg ggtgctcgga cttctgggcg cgcggcgatc gcggcggcgg ggcgaactac
     1261 ttcaactgcg ggagcgggag gcccggcgtc caccaaccgg ggttctctcg gaacgcggtc
     1321 cggtggctga tccggaccgg cgtcctgacg gagaccggcg cgctcgggac cgacacgttc
     1381 gggcccgacc cgtcgtccga cgccgccttc ctgccgacgt ggctcaccct gcgcaagcac
     1441 cgcctcacga tcgagaacct gacgaacctg gacgcactgc cgccgacggg tgcgtgggtc
     1501 gtcctcggga gcccgcgcaa cgcgaacggt tcgggggcgc cgggaacggt gttcggcctg
     1561 atcccgagcg gctagcccgg ggtccggggt ccgggggtcg cgggtcgact cagaggagac
     1621 ggacggcccg gatcgacggg agatcggtgg cggcgcgcgt ccagcgttcg cccgcgtcct
     1681 gcgagacgaa gacctcgccg tcgcccgtgc cgaacgcgac ctgggatcct cgcgcgtcga
     1741 gccaaccgga gtcgatgttg ccgtcgaacc agtcgggcag cccctcggtg cagcgctcga
     1801 acgtggcgcc cggttcgagc gcgcccctgt agaccgcgcc acggcctcct cggggaccgt
     1861 tcgagaccga gaccaggaca cggtcaccgg cgaccgcgac ggctcgcgca taggtggcgt
     1921 gcaggccctc gcgttcgatc cgccagctcg aacctccgtc gacgctcacg gcgagcccga
     1981 cggcgcccgc ggcgagcacg acgtcgggtt gggtcgggtg ggcgatgacc tggtggacgt
     2041 ccgcgtcgat gtcgatcgtc ggcgcccagc tcgcccccgc gtcgaccgag cgggggatgc
     2101 cgcccacgtg gacgttcgcg tggagccgcc ccgcgaggtc ctgggcgatc gaacggacgg
     2161 ccggcggacc accccagggc gtgtaccagc tctcccggcc ctccaccgtt tcgaacgcct
     2221 cgaccggcgc gagggtgccg tcgtcgccga agagtcggag caggtgggcc tcctccgtgc
     2281 cgacgagtac cccgtccgag gtgtcggcca ggcaggcgat ctggagctcg ctccgagcga
     2341 cgtccgtcca ggtcccgtcg ggtgcgcgcc gcacgatcgc gttgtgcccg gccaccgccc
     2401 aggcggcgcc ctcacggacg gccagcgcgg cgacctcccg ggctcggaag ctcgcctcgg
     2461 ggcgcggctc cccgccggcg agggcgaaca gcccctcgcg tgtgccgatc agcaggtccg
     2521 ccacgggatc gcctacccgc cgaacgccga ggccgcggcg cgagcgatct cgtcgcgcgg
     2581 aacgtcccgc acgtgttggg ccagacccca ccggtggccg aagggatcgg ttagctgtcc
     2641 ctgccggtcg ccccagaact gatccgtcag cggatggagc acctcggcgc ccgcgccgag
     2701 ggcgcgctcc cagagcgcct cgacctcatc cgtgaacaga tggagcacga ccggcgtccc
     2761 gccgatgctc tgcggtgaga cgacgccggc ctcggggaat tcgtcggcaa ccatcaccgc
     2821 cgaatcaccg aaccacagct ccacgtacat cagtttgcca ccgggcagtg ggagtcggtc
     2881 gcgttcctcg gcaccgaagg cggatgcgta ccactcggcg gcgcgagcgg catcccgcac
     2941 gacgatgtgc ggtgtgatgg tgtgcatccc ctcggggatc gccctgacgt ccacgtcggc
     3001 ctcctgtcga tcgggcaaca acgaggctag cgtgcctacg ggtcagggtg cgggcgtgtg
     3061 ctactcggcg tcgcggtgcc gatggtccgt ctggcgggcg atccgcgccg cggcgccggc
     3121 gatcttctgg acgacggtga agcggtcgcc ctcggagacg agctggtcga cctcggggtt
     3181 ctcgtggtcg gtagcgatcg cgaagcgcgt cgggtacccg cggacggcct cgtattcgat
     3241 gcgcgtcagg gtgatgcgtg ctcggcaggc cgggtcgctg cactcgcaca cgaacagctc
     3301 ttcggtctcg tggtccagcg cgaccgcccc gcgcgtctcg gcgatccact cgttccggtc
     3361 gcgcgaacgc gattcgttgc ggacctcctg ctcccgccag tcgctcacca tcaccatcca
     3421 gccatcagga ggacgcgagg tcgaggatct tgcggatgcc gttgtcgatc cgaagcctgc
     3481 cgccccaggt cgcgacacgg tgctcgcatg cgatcacgcc gagccaaccc gccccgtcga
     3541 tcgattcgag cgaatggact tcgaggatca cgtctcgcgg cctggctcgg gtgaccgccg
     3601 ccagtagcgt gggggccgcc tcggcgtcga gccatccccc cagcgtcagg tgcagggcgc
     3661 cgttcgactc ctcgatcagc accgagaagt cctcggaggg gaccctcatc gcgactccct
     3721 tcgtggagga acggtcctcc tagagggagc gagtctctgg gctccgatga cgtgccgccc
     3781 ggcgacggct cacggccggg atctggggcc gagaccctca cggtacgcgc tagcggcgcg
     3841 agaagcaatg gccaccaggc acggccggaa gcctggtagg cacggcatcg gctgcgatag
     3901 gctcgaacgt tggagggact gccatggacg aaggaggccc cgagcggctc cggatcctcg
     3961 tctcggacgg gcaggggcac cgcctcgatg aggtcctgcg gagcgtgacc gagctcggcc
     4021 acgaggtcgt ggggcagacg ccgctcggcg aggtcgcggc ggcgagcgcg tccgtccatc
     4081 ccgacgccgc gatcgtcatc gtcggggaga gcacggagcg cgcgctcgac ctcatcggac
     4141 ggatcgtcac cgaggccgag tgtccggtga tcgcgatcct cgacgtcgaa gacgagggct
     4201 tcatccgcaa ggccgccaag cgcgggatct tcgcctacat cacgctcggc cgggacgcgt
     4261 cggcgctgca gagctcgctc gacgtcgtgc tgtcgcggtt cgccgagtac cagggtctcg
     4321 agggcgcgtt cgcccgccgc gcgatcaccg agcgagccaa gggcatcctg atggagcggc
     4381 ac
//
LOCUS       DATBRK010000362        11396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2668066, whole genome shotgun sequence.
ACCESSION   DATBRK010000362 DATBRK010000000
VERSION     DATBRK010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2668066"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VIH70_10750"
     CDS             <1..502
                     /locus_tag="VIH70_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEY8116882.1"
                     /translation="SPNLVADPRPSGGSLFRIHRDVRFSKDKSPYKTHAGAHFQLGGK
                     GVHGPGYYLHLEPGECFVAGGMWMPEPEILQAIRQRIADEPAEWKKVRGKLDHSEETL
                     KRPPRGFDAEHPMIEDIKRKSFTSSVRLTDKQVTGTSFMTTFLRDCERISPLMAFLAS
                     AAGVPW"
     gene            632..1000
                     /locus_tag="VIH70_10755"
     CDS             632..1000
                     /locus_tag="VIH70_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8116883.1"
                     /translation="MNLNSVLIGSEDPAPLVAYYTKLFGAPAGSEGGYTWWQLGTGGF
                     TVSQHTEVKGKNAHPGRVIWNIESNDVKGDFERLKAAGAIVVQEPYAPDAGDAADAPE
                     MSVATFADPDDNYFQLVSPM"
     gene            complement(1024..1698)
                     /locus_tag="VIH70_10760"
     CDS             complement(1024..1698)
                     /locus_tag="VIH70_10760"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY8116884.1"
                     /translation="MEGTEASDPQGGVETAHGAAGIRVLVSFAAGVAAFGVAWLATPW
                     QAAVLIGWNVSATVFIAWLWFTVAGMDGEATARHAAIEDLSRSTADLILIIASVASLV
                     GVGLALLEASDTEGLEKAMIVGNASVSVILSWATVHTVFTLRYARLYYGAGGGIDFNG
                     DRAPTYTDFAYLAFTIGMTYQVSDTSIASKAIRRTALRHAYMSYLFGTVIVAMTINVV
                     AGLFNR"
     gene            1766..2386
                     /locus_tag="VIH70_10765"
     CDS             1766..2386
                     /locus_tag="VIH70_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEY8116885.1"
                     /translation="MPFGRYTVLDDGEPVGTEEFRCAPGPMGWRSFSQVQTSVPSPRA
                     ETLDFAVDAGWRLARVRIDTGEHDLLLEPAQGGDVLRGFRDRRPIEIGFGPDVHLDYF
                     TPTTNAITTHRLSGTADIDVVYLEPVTLQSSRARQRYELVGDERVQTPVGAFDATRWR
                     FTALGTDQGTDWTSDLWVAGDIVVRYERLFELEWLDPGASGPRPIA"
     gene            2388..3107
                     /locus_tag="VIH70_10770"
     CDS             2388..3107
                     /locus_tag="VIH70_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116886.1"
                     /translation="MGRRWVTAAGTIRAIAESDPDVAARIFGSPDAIALGGWPGATTG
                     RSWASSARFAEDVANDTIPDDLQVAMYDPERWDATPLDERLDPHASIEAFGTLARSKG
                     YTVLVTPHPNLVSVPGSAFAPKDGEARESAYMRSGIAEVAAANADVIETQAQTLQGDP
                     DRYRAFVAETVHIARATNRDIQVLSGLSTHPGYPATVAMLRNAWMSVRDVVDGHYLSL
                     ARLRLPEVATAFLAETVTTDP"
     gene            complement(3091..4248)
                     /locus_tag="VIH70_10775"
     CDS             complement(3091..4248)
                     /locus_tag="VIH70_10775"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEY8116887.1"
                     /translation="MTDVEVGAPSQDGELDIYARKRTKPVWRVIQILLALAVVAISFL
                     YAIPKIANYSEVWTEIRDMTPLELMFLLAATALNLVTYWWASMVSIPNLGLWQAAVNN
                     QTSTTVANTMPGGGYVSIAVSYEMYRAWGFSKTDVGLSVAVTSVLNIFAKLALPAIAL
                     LLIIVTRRASGPLIGASIVGILILIGAVVLFGLLMWKKRFAKAIGSRLGRAATWVARR
                     FRRSRTFDWGEAAVRFRRNTIDFLVERWFRLTSTTIISHLSLYLVLLLALRFVGVSDA
                     EVNWAQVLGVFAFARLLTAVPITPGGLGIVEVAYIGGLILAGRGHADVPTDVFHAQVT
                     AAVLVFRALTYGIQIPLGAITYAIWRTNTSWRKPAPIAAPVPRPSPVRGPS"
     gene            4759..5058
                     /locus_tag="VIH70_10780"
     CDS             4759..5058
                     /locus_tag="VIH70_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116888.1"
                     /translation="MVTIAVDAVPAGARRRPASRRSKGTGIGGTSVARHPFVLPDVRD
                     LGSDGEDGEEHAPDQDDDPFGLDHDSLANAATLALVEAQTFHQRKGTHRGGSPSS"
     gene            complement(5114..5419)
                     /locus_tag="VIH70_10785"
     CDS             complement(5114..5419)
                     /locus_tag="VIH70_10785"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8116889.1"
                     /translation="MKHVRIATYEIKKGSFQELADVAQDGMLREFRNQPGFIRYGLAD
                     SGDKTCVSLSLWETRKDAESASRISETWDREHIADRAELRTNQVGDLAFYEGVPAKV"
     gene            5884..6096
                     /locus_tag="VIH70_10790"
     CDS             5884..6096
                     /locus_tag="VIH70_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116890.1"
                     /translation="MRPRDPEATTNFFRTELGKTLRVDETDEGAISVDILKEGVWSEA
                     PRGMIGLRLSPGSRGLTATEILALPT"
     gene            complement(6125..9664)
                     /locus_tag="VIH70_10795"
     CDS             complement(6125..9664)
                     /locus_tag="VIH70_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8116891.1"
                     /translation="MPPPRTCPDCSAANPAEARFCLNCGAKLDDFTAIGPDNHLEPYL
                     PMELLAKLEAARAGRAMEGERRVVTMLFCDVKGSTAMAETLDPEDWAEIMNGAFERLI
                     APVYRYEGTLARLMGDAIFAFFGAPIAHEDDPQRAVQAGLDIIAGIAPYRERISAERG
                     LDLDVRVGINTGSVMVGQVGSDLRLEYTAMGDAVNVAARMEQTADPGTVQITAETHRL
                     VEALFEVVARGRIEVKGKAEPVSVFRVLGRKAGVTEARSLRGSPLVGREREMDQLRDA
                     IEEAQSGGGRIVSLIGEAGLGKSRLIQETLAEWTRRRPEDSRIGGEVHRLWESWQCVS
                     YDRTRPYGQYRRVLARLAGIEDTDAPDEVRRKLVATMEPGSEDEWLEPHMRVWRSLFG
                     VTEPDEEPLEGQAFRDAIMELVPAATRAFGADEPRLLVFEDLHWCDEASMDVLIETAR
                     LVDDLPCLFLFAYRPDRRAQSWRLKQWLETEYPHRSTELALEPLSDDDSGRLIDALIP
                     GGDPEVRTQILERTDGNPLFVEEVAVAMLEHDELAIPDTLQALFTTRLDAVDEDCKHT
                     LQLASVIGRSFSEPVLRAVAGEADLTSSLRTLERMGLISETARTPDREYAFHHSLTQD
                     ATYGTILLRRRRELHERVGEVFEERYANRLDEFAPVLAHHFREAGDDERMLRYATLAG
                     DNAARLYANAESAAQYATAIEAAKRRGATAEAIGHLYPSRGRVLELSGRYDEAVEAYE
                     EMATLARDAGNRGGVLSAEMALTTIHATPTPVFDSSKGRVLAERTIALARELGDRSAE
                     SKALWNLMILNVFGGGDADEAVEAGERSLEIARELNAREQIAFTLNDLWRPYAAVGDV
                     AAARACLEEARPIWREIGNLPMLCENLIGTAALLTLAGEPQEALALYDEAYATSGEIG
                     NPWGQAYALFNAYLVDVEQGNVGRAMARMRECIQRSEEAGFLIPQSAARADLGVQYAR
                     LGQFERATELADRGLEIAKGRTPLAIPMAMVSKAEILFLAGESEQAESMIAEANLGRL
                     PGPVRGAAGAHVDILRGQLAAARGDYVRAVEIADAVTAWLHRHELRQFLPAALLLKSR
                     ALIASERQEEADPLLREARSRAEDMGFRRLLWEIDWELSRVAADRGDPAEAAALRAEA
                     ASTVSAITVTIDDQELRASFLALPDVRAALSER"
     gene            complement(9803..10234)
                     /locus_tag="VIH70_10800"
     CDS             complement(9803..10234)
                     /locus_tag="VIH70_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEY8116892.1"
                     /translation="MATKQARVSWKGDLENGTGTIEQVGSGAIGSLAVSWAARTGDEG
                     GMTSPEELIAAAHASCFSMAFSARLAKAGTPPDRLEVSAAVTFVPGTGITTSALDVTG
                     VVPGISQEDFVTLAEDAKENCPVSSALKGNVEMSVMARLAS"
     gene            10365..10694
                     /locus_tag="VIH70_10805"
     CDS             10365..10694
                     /locus_tag="VIH70_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HEY8116893.1"
                     /translation="MPQTAWSAKRERQYEHIKQGLEERGRDEDTAEEIAARTVNKERA
                     RSGEARTSSPTSTRDISSGRRGGLRSHRGPGGRTKAQLYREARDKHVEGRSKMTKAQL
                     QRAVDRD"
     gene            10707..10898
                     /locus_tag="VIH70_10810"
     CDS             10707..10898
                     /locus_tag="VIH70_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116894.1"
                     /translation="MPSVQGLSKTQANYRKAENPQIRCGECKFMFPRLSIGGCRFVRG
                     VIHNDDTCDEFKPRDATGP"
     gene            complement(10926..>11396)
                     /locus_tag="VIH70_10815"
     CDS             complement(10926..>11396)
                     /locus_tag="VIH70_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8116895.1"
                     /translation="EDAETISAYRSDPDVNRQQGWDRTDPAGVLADIVEMSTRVPGEP
                     GGWVQFTVEEREGGRIVGDVGFAVADGEPNVIKVGYTISPEFQGRGYATEAIRALVDY
                     SFETLGAEIVRAHASAENASSIRVAEKVGMGLVERVEYREGDEVWLGVRYEVRR"
BASE COUNT         1790 a         4088 c         3833 g         1685 t
ORIGIN      
        1 cagccccaac ctcgtcgccg atccgcggcc gtcgggaggt tcgctgttcc ggatccaccg
       61 cgacgtccgc ttctccaagg acaagagccc gtacaagacg cacgcgggcg cgcacttcca
      121 gctcggcggc aagggcgtcc atgggcccgg ctactacctg cacctcgagc cgggcgagtg
      181 cttcgtcgcc ggcgggatgt ggatgccgga gccggagatc ctccaggcga tccggcagcg
      241 gatcgcggac gagccggccg agtggaagaa ggtccggggc aagctcgacc actcggagga
      301 gacgctcaag cgcccgccgc gtgggttcga cgcggaacac ccgatgatcg aggacatcaa
      361 gcgcaagagc ttcacctcct cggtccggct caccgacaag caggtgaccg gcacgagttt
      421 catgacgacg ttcctgcgcg actgcgaacg gatctcgccg ctgatggcgt tcctcgcgtc
      481 ggccgccggc gtgccctggt agagcggtcc gcctcggccg tccgcctcag ccgtgtggca
      541 ccgatgactt ccgccgtccg gctccgtctg ggtttcgagt ccatcgaacg gggatagctc
      601 ctcagggaga tcccatgagg aaggaggccg catgaacctc aacagcgtcc tgatcgggag
      661 cgaggatccc gcgccgctgg tggcgtacta cacgaagctc ttcggggccc ccgcaggcag
      721 cgagggcgga tacacgtggt ggcagctcgg gaccggcggg ttcaccgtct cccagcacac
      781 cgaggtcaag ggcaagaacg ctcatccggg tcgggtcatc tggaacatcg aatccaacga
      841 cgtgaagggc gacttcgaac gcctgaaggc cgcgggcgcg atcgtcgtcc aggagcccta
      901 tgcgcccgac gccggcgacg cggccgacgc gcccgagatg tcggtcgcca cgttcgccga
      961 ccccgacgac aactacttcc aactcgtcag cccgatgtag cgggtggccg cgctcgagtg
     1021 cggctaccga ttgaacaacc cggccacgac gttgatcgtc atcgcgacga tgacggtccc
     1081 gaacaggtac gacatgtagg cgtggcgcag ggccgttcgc cggatcgcct tcgacgcgat
     1141 cgacgtgtcc gagacctggt aggtcatccc gatcgtgaac gcgaggtagg cgaagtcggt
     1201 gtaggtgggg gcccggtccc cgttgaagtc gatcccccct cccgcgccgt agtacagcct
     1261 cgcgtaccgc agcgtgaaca ccgtgtggac ggtcgcccag gacaggatga cgctcaccga
     1321 ggcgttcccg acgatcatgg ccttctcgag gccctcggtg tcggaggcct cgagtagcgc
     1381 gaggcccacc ccgaccaggc tcgccacgct cgcgatgatc aggatcaggt cggcggtgga
     1441 ccgcgagagg tcctcgatcg ccgcgtgcct cgccgtggcc tcgccgtcca tcccggccac
     1501 cgtgaaccag agccaggcga tgaagacggt cgccgagaca ttccatccga tcagcaccgc
     1561 cgcctgccac ggcgtcgcga gccaggcgac gccgaacgcc gcgacgcccg ccgcgaacga
     1621 caccaacacc cggatgcccg ccgcgccgtg cgcggtctcg acgccaccct gcggatcgct
     1681 cgcctccgtc ccctccaccg ggcccacgat acgcggggcc cttcccacac gcaccggagc
     1741 gggaacacgc cgatacgctg ggtcgatgcc gttcggtcgc tacacggtgc tggacgacgg
     1801 ggaaccggtc gggaccgagg agttccggtg cgcccccggt ccgatgggct ggcgttcgtt
     1861 cagccaggtc cagacctcgg tcccatcacc ccgcgccgag acgctggact tcgcggtgga
     1921 cgccgggtgg cggctggcgc gcgtccggat cgacaccggg gagcacgacc tgctgctcga
     1981 accggcgcag ggaggcgacg tcctccgggg cttccgggac cgccggccga tcgagatcgg
     2041 gttcggcccc gacgtccacc tcgactactt cacgcccacc acgaacgcga tcacgacgca
     2101 ccgcctctcg ggcacggcgg acatcgacgt tgtctatctc gaaccggtca cgcttcagtc
     2161 gagccgcgcc cgtcagcgct acgagctcgt gggggacgag cgcgtgcaga cgccggtcgg
     2221 cgccttcgac gcgacccggt ggcgcttcac cgcgctcgga acggaccaag gaacggactg
     2281 gacgtcggac ctgtgggtcg cgggtgacat cgtcgtgcgc tacgagcgcc tgttcgagct
     2341 cgagtggctc gacccgggtg cctcgggtcc acgcccgatc gcctgacatg ggacggcgct
     2401 gggtcacggc cgccggcacg atccgggcga tcgccgagag cgatccggac gtcgccgctc
     2461 ggatcttcgg ctcccccgat gcgatcgcgc tgggcggctg gccgggagcg acgaccggtc
     2521 gctcctgggc gagctccgcg cggttcgccg aggacgtggc gaacgacacg atcccggacg
     2581 acctccaggt cgcgatgtac gacccggagc gctgggacgc gacaccgctg gacgaacggc
     2641 tcgacccgca cgcgtcgatc gaggccttcg gtacgctcgc gcgttcgaag ggatacacgg
     2701 tcctagtcac gccccacccg aacctcgtct cggtccccgg aagcgcgttc gcgccgaagg
     2761 acggcgaggc gcgcgagtcg gcgtacatgc gctccggcat cgccgaggtg gcggccgcga
     2821 acgcggacgt gatcgagacg caggcccaga cgctccaggg tgatccggac cgctaccgag
     2881 cgttcgtcgc cgagacggtg catatcgcgc gcgcgaccaa tcgggacatc caggtgctct
     2941 ccggtctgtc gacgcatccc gggtacccgg cgacggtcgc gatgttgcgg aacgcctgga
     3001 tgtccgtccg cgacgtcgtc gacgggcact acctgtcgct cgcgcgcctg cgcctgcccg
     3061 aggtggccac tgcgttcctc gcagagacgg tcacgacgga cccctgaccg gcgaaggtcg
     3121 gggcaccggg gccgcgatcg gcgccggctt gcgccagctc gtgttcgtac gccagatcgc
     3181 gtaggtgatc gccccgagcg ggatctggat cccgtaggtg agcgcccgga agaccagcac
     3241 ggccgcggtc acctgggcgt ggaagacgtc ggtcggcacg tccgcatgac cccggccggc
     3301 caggatcagc ccgccgatgt acgcgacctc gacgatcccg agcccgcccg gcgtgatcgg
     3361 caccgcggtg agcaaccgag cgaacgcgaa cacgcccagc acctgcgccc agttgacctc
     3421 ggcgtccgac acgccgacga accgaagcgc gagcagcagc acgagataca acgagaggtg
     3481 gctgatgatc gtggtgctcg tcagccggaa ccagcgttcg acgagaaagt cgatcgtgtt
     3541 ccgccggaac cgcaccgccg cctcgcccca gtcgaacgtt cgcgatcggc ggaaccggcg
     3601 cgcaacccac gtcgccgctc gcccgaggcg ggacccgatc gccttcgcga accgcttctt
     3661 ccacatcagc agcccgaaca gcaccaccgc cccgatcagg atgaggatcc cgacgatcga
     3721 ggccccgatc agcggcccgc tcgcgcggcg ggtcacgatg atcaagagca gcgcgatcgc
     3781 cgggagcgcg agcttcgcga agatgttgag gacggaggtc acggcgaccg agaggccgac
     3841 gtccgtcttc gagaagcccc acgcccggta catctcgtag ctgacggcga tagagacgta
     3901 gccgcccccc ggcatcgtgt tcgcgaccgt cgtcgacgtc tggttgttga ccgcggcctg
     3961 ccacagcccc aggttcggga tcgacaccat gctcgcccac cagtaagtga cgaggttcag
     4021 cgccgtcgcc gcgagcagga acatgagctc gagcggcgtc atgtccctga tctcggtcca
     4081 gacctcggag tagttcgcga tcttggggat cgcgtacagg aagctgatcg cgacgaccgc
     4141 gagggcgagc aggatctgga tgacgcgcca gaccggcttc gtgcgcttgc gcgcgtagat
     4201 gtcgagctcg ccgtcctgcg acggtgcgcc gacctcgacg tcggtcacct cgaggcccgg
     4261 aggcgtcgag gttcctcggt cgcgcgtcct atgcgtaccg gccgcacccg aggcaggtgt
     4321 accagcccgc ccgatcctgc agcgcgaacg tcgcctggca tccgcagtgc ctgcacgtgc
     4381 gcccgacgtt cgaagtcaat caccgcgagc cagggaccca gcatcgggct ccccttcctg
     4441 gaggacgcgc gccctccggc ataggagcga gtctctgggc tccgatgcgc ccggtcggca
     4501 ggcgccagcg ggccggggga tctgaggtcc gcgcaacgta ccctacgact cgttgtccga
     4561 gcacgcaatg ggacgaccgc ccgatgccgc gcaccagagt cggtatgacg gcgtctccgt
     4621 cctacggttg tggttccacc actggtggat cctcccaaga cgggcgcccg cgcgaggacc
     4681 ttcgcgacct tgcctttccc gggtccctga gaccgaggag gcagccctgg tccactggcc
     4741 gaagaccatc gtggcaacgt ggtgacgatc gcggtcgacg cggtgcccgc cggagccaga
     4801 cgccggccgg ccagccgccg atccaaggga acgggaatcg gcggaacgag cgtagcccgt
     4861 catcccttcg tgcttccgga tgtgcgcgat ctcggatcgg atggagaaga cggcgaagag
     4921 catgcgcccg atcaggacga cgatcccttc ggtctcgatc atgactccct cgcgaacgct
     4981 gcgaccttgg cgctggtcga ggctcaaacc tttcaccaaa gaaaggggac ccaccgaggt
     5041 ggatcccctt cttcctgacg gccgccgctc gtggatcgca cgagctggcc gggttcaacg
     5101 accgggcggt cgatcagacc ttggcgggaa cgccttcgta gaaggcgagg tcgccgacct
     5161 gattcgtcct cagctcggcc cggtccgcga tgtgctcgcg gtcccaggtc tcggagatgc
     5221 gggacgcgct ctcggcgtcc ttgcgtgtct cccacaggct gagcgagacg caggtcttgt
     5281 cgcctgagtc ggccaggccg taccggatga aaccgggctg gttccggaac tcgcgcagca
     5341 tcccgtcctg ggcgacgtcg gcgagctcct ggaacgagcc cttcttgatc tcatacgtag
     5401 cgatacgaac gtgcttcatg cgtgttctcc tttgctcgtg tgcccctgac caaccagggc
     5461 caacgtgagc gagatccgag cggatacgct agggcatcag gctttcgctg gagccagcga
     5521 gatgggacag gatcggtgga gacgatcccc gtgcgtcctc ttctcccgac aggtacgggg
     5581 gtcgcgtctc cctggagtgt acgtctttcc ctcgtgcaca cgaccgcggg tttcgcacga
     5641 tctggaaccc gaaccttcgt ccggaccgct ctcagagtga gatgatgatg gcaccacacc
     5701 cacagggacc gtttgcgtcg aggccgtgag cagcccctcc gatcgaggag gcacgatgac
     5761 gacgctcacg gaccctgtcc acgtcgacgt tccggagcga gctgcggtcc aaccggcggc
     5821 tcccctagat cctccacgcg aaacggaaca accggacacg accgccgaac ggcgggctgg
     5881 gtgatgcggc cccgagaccc cgaagcgacg acgaacttct tccgaaccga gctcggcaag
     5941 acgctgcgcg tcgacgagac cgacgaggga gcgatcagcg tcgacatcct caaggaaggc
     6001 gtgtggtccg aggcgccgcg cgggatgatc gggctccgct tgtcgccggg cagccggggg
     6061 ctgaccgcca ccgagatcct cgcgctcccg acctagacac acgatcgatc gccgccgttc
     6121 gacatcagcg ctcggagagc gcggccctta cgtcggggag agcgaggaag ctcgcgcgga
     6181 gctcctggtc gtcgatcgtc acggtgatcg cggagacggt cgacgctgcc tccgcccgca
     6241 gggccgcagc ttcggcgggg tcgccacgat cggccgcgac acggctcagc tcccagtcga
     6301 tctcccacag caggcgtcga aagcccatgt cctcggcgcg ggacctcgcc tcccgaagca
     6361 gggggtccgc ttcctcctgg cgctcggagg cgatgagcgc ccgcgatttg agcagcaggg
     6421 ccgcggggag gaactgacgc agctcgtgcc gatgcagcca cgcggtcacg gcgtcggcga
     6481 tctccacagc ccggacgtag tctccccgtg ccgcagcaag ctgccctcgg aggatgtcga
     6541 cgtgcgcgcc cgcggcgcct cggaccgggc cgggcagccg cccgaggttc gcctcggcga
     6601 tcatggactc cgcctgctcg gactcgcccg cgaggaacag gatctcggcc ttcgacacca
     6661 tcgccatggg gatggcgagg ggcgtccgac ccttcgcgat ctcgagtccc cggtccgcga
     6721 gctctgtcgc ccgctcgaac tgcccgagcc gcgcgtactg gacgcccaga tcggcgcgcg
     6781 ccgccgactg cgggatcagg aatccggcct cctcggagcg ctggatgcac tcacgcatcc
     6841 tggccatggc ccggcctacg ttgccctgtt ccacgtccac gaggtacgcg ttgaacaggg
     6901 cgtaggcctg cccccacggg ttgccgatct ccccggacgt tgcgtaggcc tcgtcgtaga
     6961 gggccagggc ttcctgcggc tcaccggcga gcgtcaggag cgcggcggtg ccgatgaggt
     7021 tctcgcacag catgggcagg ttgccgatct cccgccagat cggcctcgcc tcctccagac
     7081 aggcgcgggc cgcggcgacg tcaccaacgg cggcgtacgg acgccacagg tcgttcaggg
     7141 tgaaggcgat ctgctcccgg gcgttcagct cgcgcgcgat ctccagcgac cgctcccccg
     7201 cttcgacggc ctcgtcggcg tcgccgccgc cgaagacgtt caggatcatc aggttccaca
     7261 gggccttcga ctccgccgag cggtcgccga gctcgcgcgc gagcgcgatc gtgcgctcgg
     7321 ccagcacccg ccccttggag gagtcaaaca ccggcgtcgg cgtcgcgtgg atcgtggtga
     7381 gggccatctc cgcgctgagc acgcctccgc ggttccccgc gtcccgcgcg agcgtcgcca
     7441 tctcctcata ggcctcgacc gcctcgtcgt agcgaccgga gagctcgagc acccggccgc
     7501 gactcggata caggtggccg atggcctcgg ccgtcgcccc gcgccgcttg gccgcctcga
     7561 tcgccgtcgc gtactgggcg gcggactcgg cgttcgcgta gagccgcgcg gcgttgtcgc
     7621 cagcgagcgt cgcgtaccgc agcatccgct cgtcgtcccc cgcctcccgg aagtggtggg
     7681 ccagcaccgg cgcgaactcg tcgaggcggt tggcgtagcg ctcctcgaag acctcgccca
     7741 cacgttcatg cagctcgcgc cggcgccgga gcaggatcgt gccgtaggtc gcgtcctgtg
     7801 tgaggctgtg gtggaacgcg tactcgcggt cgggggtccg ggcggtctcg gagatcaggc
     7861 ccatgcgctc gagcgttcgg agcgaggagg tgaggtccgc ctcaccggcc acggcccgca
     7921 ggacaggctc ggagaacgag cggccgatca ccgaggcgag ctgcagcgtg tgcttgcagt
     7981 cctcgtcgac cgcgtcaagc cgcgtggtga acagcgcctg cagcgtgtcc gggatcgcga
     8041 gctcgtcgtg ttcgagcatg gcgaccgcga cctcctcgac gaacagcggg ttgccgtcgg
     8101 tgcgctcgag gatctgggtt cggacctcgg ggtccccgcc ggggatcagc gcgtcgatca
     8161 gccggccgct gtcgtcgtcc gagagcggct cgagcgcgag ctccgtcgat cggtgcgggt
     8221 actcggtctc gagccactgc ttgagccgcc acgactgcgc ccggcggtcc gggcggtagg
     8281 cgaacaggaa caggcacggc aggtcgtcca cgagccgcgc ggtctcgatc agcacgtcca
     8341 tcgaggcctc gtcgcaccag tgcaggtcct cgaacaccag gagccgcggc tcgtcggcgc
     8401 cgaacgcccg cgtcgcggcc ggcaccaact ccatgatcgc gtcgcggaac gcctgcccct
     8461 ccagcggctc ctcatccggc tcggtgacgc cgaacaagga ccgccacacg cgcatgtgcg
     8521 gctcgagcca ctcatcctcc gaaccgggct ccatcgtcgc gaccagcttc cggcggacct
     8581 cgtccggcgc gtcggtgtcc tcgatcccgg cgagccgcgc cagcacgcgc cggtactgcc
     8641 cgtacgggcg cgtcctgtcg tacgagacgc actgccagct ctcccagagg cggtggacct
     8701 cgccgccgat gcgcgagtcc tcgggccgtc ggcgggtcca ctcggccagc gtctcctgga
     8761 tcagccggga ctttccgagc cccgcctcgc cgatcagcga aacgatccgc ccgccccccg
     8821 actgcgcctc ctcgatcgcg tcccggagct ggtccatctc gcgttcgcgt ccgaccagcg
     8881 gtgagccgcg gaggctccgt gcctccgtca ccccggcctt cctcccgagg acccggaaca
     8941 ccgacacggg ctcggccttg cccttgacct caatccggcc ccgcgccacg acctcgaaga
     9001 gcgcttcaac cagccggtgc gtctcggcgg tgatctgcac ggtgccgggg tcggccgtct
     9061 gctccatgcg cgcggccacg ttcaccgcgt cgcccatcgc cgtgtactcg aggcggagat
     9121 ccgagccaac ctgcccgacc atcaccgagc ccgtgttgat gccgacccga acgtcgagat
     9181 ccagcccgcg ctcggcgctg atccgttccc tgtagggggc gataccggcg atgatgtcga
     9241 gccccgcttg gaccgcgcgc tgggggtcgt cctcgtgcgc gatcggcgcg ccgaagaacg
     9301 cgaagatcgc gtcgcccatc agacgggcga gcgtgccctc gtaccggtag acgggggcga
     9361 tcagccgttc gaacgccccg ttcatgatct ccgcccagtc ctcggggtcc agggtctcgg
     9421 ccatcgcggt cgaacccttg acgtcgcaga acagcatggt gacgacccgc cgctcaccct
     9481 ccatggcccg tcccgcgcgt gcggcctcga gcttggcgag cagctccatg ggcaggtagg
     9541 gctcgaggtg gttatcgggg ccgatcgcgg tgaagtcgtc aagcttcgca ccgcagttca
     9601 ggcagaagcg ggcctccgcc ggattggcgg ccgagcagtc ggggcaggtc cttggcggcg
     9661 gcatgggttc gggcgataac gatagccgcg gtacacatcg ccccggctcg agtgcgtgcc
     9721 ggtgtgacca ggcgtgggtg ctctcgcccc agccaaccgt ccggttcgtg atcgcaggga
     9781 accgaccgtg gccgccccct cgctacgacg cgagccgcgc catgacgctc atctccacgt
     9841 tgcccttgag cgcgctcgag accgggcagt tctccttagc gtcctcggcg agggtgacga
     9901 agtcctcctg cgagatcccc ggcacgacgc ccgtcacgtc gagcgccgac gtcgtgatcc
     9961 cggtccccgg cacgaacgtg accgcggccg agacctcgag ccggtccggc ggcgtcccgg
    10021 ccttcgccag ccgcgccgag aacgccatcg agaagcacga ggcatgggcc gccgcgatga
    10081 gttcctcggg gctggtcatc ccgccctcgt cgccggtgcg tgccgcccac gagaccgcga
    10141 gcgacccgat cgccccgctg cccacctgct cgatcgtgcc ggtcccgttc tccaggtcgc
    10201 ccttccaact cacgcgtgcc tgtttggtcg ccatcgcctc ctcctttccc accagctaat
    10261 cgcgaccgag ccgcttcggc aacccggggc gtgaagtcgt gcaggagtgg gtagctcgac
    10321 gctagaaggg gatggcacga cgcgtcagca cggaggagga tgtcatgccg cagacggcgt
    10381 ggagcgccaa gcgcgaacga cagtacgagc acatcaaaca gggcctcgag gagcgaggtc
    10441 gcgacgagga cacggcggag gagatcgccg cccgaacggt caacaaggaa cgggcccgct
    10501 ccggcgaggc gcggacctcg agcccgacct cgacccggga catctcgtcc ggccggcgcg
    10561 gtggcctgcg gtcgcaccgc gggcccggcg gtcgcacgaa ggcgcagcta taccgggagg
    10621 cgagggacaa gcacgtcgag ggacgatcga agatgacgaa ggcgcagctc cagcgcgccg
    10681 tggaccgcga ctgaggaggc gacctcgtgc cgagtgtgca ggggctctcg aagacgcagg
    10741 cgaactaccg caaggccgag aacccgcaga tccgctgcgg cgagtgcaag ttcatgttcc
    10801 cgcgcctgtc gatcggcggg tgccgcttcg tccgcggcgt gatccacaac gacgacacct
    10861 gcgacgagtt caagccgcgc gacgccaccg ggccctgacg gtcgttcggg acgtctgccc
    10921 gagtctcagc gacggacctc gtaccgcacc ccgagccaga cctcgtcgcc ctcgcggtac
    10981 tcgacgcgtt cgacgagccc catcccgacc ttctccgcga cccggatcga ggaggcgttc
    11041 tccgcgctgg cgtgtgcgcg gacgatctcg gcgccgagcg tctcgaagga gtaatccacg
    11101 agcgcgcgga ttgcctcggt cgcgtacccg cgcccctgga actcgggcga gatcgtgtac
    11161 ccgaccttga tcacgttcgg ttcgccgtcg gcgaccgcga acccgacatc gcccacgatc
    11221 ctgccgccct cgcgctcctc gaccgtgaac tgcacccagc cgccgggctc cccggggacc
    11281 cgcgtgctca tctcgacgat gtcggccagg acaccggcgg gatcggtgcg gtcccacccc
    11341 tgctgccggt tcacgtccgg gtcgctgcgg tacgccgaga tcgtctcggc atcctc
//
LOCUS       DATBRK010000363         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_213161, whole genome shotgun sequence.
ACCESSION   DATBRK010000363 DATBRK010000000
VERSION     DATBRK010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213161"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VIH70_10820"
     CDS             complement(<1..648)
                     /locus_tag="VIH70_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8116896.1"
                     /translation="MPALAHVPVQRGPTARLRFLAFDVEPRRIVFDVLTFRAVVSQGM
                     SKRKEATPVEHLILRMAADHPFVTTGEIAAAARVTRQAAHYHAKRMVANGLLTREGAG
                     RGSRYLLASDLARAYRLEGLEEHVVWSELEGSIPTEQTSSKAVRIATYAFTEMLNNAI
                     DHSGGTSVFVRAWFEGAELGFEVGDDGVGAFRSIRERMDLPDEWAALQELVKGKAT"
     gene            complement(597..>1502)
                     /locus_tag="VIH70_10825"
     CDS             complement(597..>1502)
                     /locus_tag="VIH70_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY8116897.1"
                     /translation="AQRRTLDGLIGTGERPVFAADAVPRVRDRIEEAARAFEPPEPLW
                     LGKSNLSDLHRCPGLFDAVRAGERAPFAFSARTAAGRLAHKAIELEVAGREERDPHDL
                     VEQAAERLTEDGAFSAYWVGLDALHRDENLMDAAKTLDLFRSTMPPLRQMRRELAPST
                     EWHVRVELLGGTVVLSGQLDLVLGSGRAAGSARATRLAIDLKTGRAWPEHAEDMRFYA
                     LLLGLRFGIPPYRVATLYLDSGEWQAEDVDERVLDRAADRVIEAIRAAAASAAGRPGA
                     LTPGPYCTWCPRSLTCPSSAARQPA"
BASE COUNT          229 a          545 c          502 g          226 t
ORIGIN      
        1 cgtcgccttg cccttcacga gctcctgcag ggccgcccac tcgtcgggaa gatccatgcg
       61 ttcccggatc gaccggaagg cccccacgcc gtcgtcgccg acctcgaacc cgagctccgc
      121 gccttcgaac caagcccgca caaagacgct cgtaccaccc gaatggtcga tggcattgtt
      181 gagcatctcc gtgaaggcat aggtcgcgat ccgtacggct ttcgatgagg tctgttcggt
      241 ggggatcgag ccctcgagct cgctccacac cacgtgctcc tccagtccct cgaggcgata
      301 ggcgcgggcg agatcgctcg cgagcagata cctcgagccg cggccggcac cctctcgggt
      361 gagtagcccg ttcgcgacca tacgcttcgc gtgatagtgg gcagcctgtc gggtgaccct
      421 tgcggcggcg gcgatctccc ccgtggtgac gaaagggtgg tcggctgcca tccgtaggat
      481 cagatgctcc accggggtgg cctctttacg ttttgacatt ccttgggaga ctaccgccct
      541 gaatgtcaaa acgtcaaaaa cgattcgccg cggctcaacg tcaaaagcta agaacctcag
      601 gcgggctgtc gggccgcgct ggacgggcac gtgagcgagc gcgggcacca cgtgcagtag
      661 gggccgggcg tgagcgcccc cgggcggccg gcggcggagg ccgccgccgc gcggatcgcc
      721 tcgatcacgc gatccgcggc gcggtccagg acgcgttcgt cgacgtcctc cgcctgccac
      781 tcgcccgaat ccaggtacag cgttgcgacg cggtacggcg ggatcccgaa ccgcagcccg
      841 agcaacagcg cgtagaagcg catgtcctcg gcgtgctcgg gccacgcgcg tcccgtcttc
      901 aggtcgatcg cgagccgcgt ggcccgggcc gatccggccg cgcgacccga accgaggacc
      961 aggtcgagct gccccgacaa cacgaccgtc cccccgagga gctcgacgcg cacgtgccac
     1021 tcggtggagg gcgcgagctc gcgccgcatc tgtctgagcg gcggcatcgt gctgcggaac
     1081 agatcgaggg tcttcgcggc gtccatcagg ttctcgtctc tatgcagcgc gtcgagcccg
     1141 acccagtacg cggagaaggc gccgtcctcg gtcagccgct cggccgcctg ttcgacgaga
     1201 tcgtgcgggt cgcgctcctc gcgacccgcc acctcgagct cgatcgcctt gtgcgcgagc
     1261 cggcccgccg ccgtgcgggc cgagaacgcg aacggtgcgc gctcgcccgc ccggaccgcg
     1321 tcgaacagac ccgggcagcg gtggaggtcg ctcagattcg acttgccgag ccacagcggc
     1381 tccggcggct cgaacgcgcg ggccgcctcc tcgatcctgt cgcgcacccg aggcaccgcg
     1441 tcggccgcga acaccggccg ttcgccggtt ccgatcaggc catcgagcgt ccggcgctgg
     1501 gc
//
LOCUS       DATBRK010000364         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1944132, whole genome shotgun sequence.
ACCESSION   DATBRK010000364 DATBRK010000000
VERSION     DATBRK010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944132"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2050
                     /locus_tag="VIH70_10830"
     CDS             <1..2050
                     /locus_tag="VIH70_10830"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEY8116898.1"
                     /translation="IQTLRAVGRSLEFVGQQARVRVAPTPALPPLMLAAVTAVWTAAF
                     ASHALAIRAGSPLLAILPPIALAGFADTVLEDGARPFYAIVLLAAALAVVFADGVRRV
                     RQWGPVWSGSRTRRLGTSVRGSRPVAFAVIAAAVLVPGLLPGFRSDPLVDFSTAGDEG
                     AGLDPFVSIHAQLTDDAPVRDLFEVQTSDPQYWRLLTLDEFDGQDWRSSDPDGSAGQI
                     VSVPTLLPQAPGFSFPGDTPPAQNFTFRILTDFDNAHALPIAQTPVEITAGDLGDVRW
                     DPTRGQAFTDGGLEAGLEYGISSRIVVPTAEQLDQVQDLTALQYGRWTELPADLDPRI
                     EEIANDWTRGEDSDYDKVLAIQRHFHTDGFRYDADVEVAEDADALLSFLTQTKAGFCQ
                     QYATTMAVLVRELGLPARIAIGYQAGTARDDGTYLVQSKNAHAWVEVFFEGYGWLQFE
                     PTPGHGIHPNAEVGTYLNPARGTSNPEDQPGGATGATGLGGGSGAECVAAANLSPRER
                     QLLCQVERKPQRGPGDFVPLPSVGAAGTPDGTGYSVPYRLIFLGLLLLLGVLLVVVPI
                     AKSAWRRRLLRRSRDPREHVLAAYRVFDGEAADLGLGRREGETLDEHRARLAATIAFT
                     DGYLGRLTTQATRAAYAADAPTPEEARSAVQDAHASIRELRRDAGLIRRIVGAYRPGV
                     "
BASE COUNT          282 a          779 c          777 g          305 t
ORIGIN      
        1 gatccagacg ctccgggccg tggggcgctc gctcgagttc gtcgggcagc aggcgcgcgt
       61 gcgcgtggcg cccacgcccg cgctccctcc gctgatgctc gcggcggtga cggccgtatg
      121 gaccgcggcc ttcgcgtcgc acgcgctcgc gatccgcgcc gggagcccgc tcctggccat
      181 cctgccgccg atcgcgctcg ccgggttcgc ggacacggtg ctcgaggacg gggcccgtcc
      241 cttctatgcg atcgtgctgc tcgccgccgc gcttgcggtg gtgttcgcgg acggcgtgcg
      301 tcgggtgcgt cagtgggggc cggtgtggtc gggttccagg acccgacgcc tcggtacctc
      361 ggtccggggc tcgagaccgg tcgcgttcgc ggtgatcgcg gccgcggtgc tcgtcccggg
      421 tctgctcccg gggttccgtt cggatccgct cgtcgacttc tcgaccgccg gcgacgaggg
      481 cgcgggcctg gatccgttcg tctcgatcca cgcgcagctc acggacgacg cgccggtccg
      541 ggacctgttc gaggtccaga catccgaccc gcaatactgg cgcctgctca cgctggacga
      601 gttcgacggg caggattggc ggagcagcga cccggacggc tcggcggggc agatcgtatc
      661 cgtcccgacg ctcctgccgc aggcgccggg cttcagcttc ccgggggaca cccctcccgc
      721 gcagaacttc acgttccgga tcctgacgga cttcgacaac gcccacgcgc tgccgattgc
      781 gcagacgccg gtggagatca cagcggggga cctcggcgac gtgaggtggg atccgacccg
      841 cggccaggcg ttcacggacg gcgggctcga ggccgggctc gagtacggga tcagctcccg
      901 catcgtcgtg ccgaccgccg agcagctcga ccaggtccag gacctcaccg cgctccagta
      961 cggacggtgg accgaactcc ccgccgacct cgatccccgg atcgaggaga tcgccaacga
     1021 ctggacgcgg ggcgaggact cggactacga caaggtgctc gcgatccagc ggcacttcca
     1081 caccgacggg ttccgctacg acgccgacgt cgaggtcgcg gaggacgccg acgccctgct
     1141 gtcgttcctg acgcaaacca aggccggctt ctgccagcag tacgccacga cgatggcggt
     1201 gctcgtccgc gagctcggtc tgccggcgcg gatcgcgatc gggtaccagg ccggcaccgc
     1261 acgggacgac ggcacgtacc tcgtgcaatc caagaacgcc cacgcctggg tcgaggtctt
     1321 cttcgagggg tatgggtggc tccagttcga gcccaccccc gggcacggca tccaccccaa
     1381 cgccgaggtc ggcacctacc tgaaccccgc cagggggacg tcgaaccccg aggaccagcc
     1441 cgggggcgcg accggtgcga ccggcctcgg cggcgggagc ggagcggaat gcgtcgcggc
     1501 ggccaacctt agcccgcggg aacgccagct cctgtgtcag gtcgaacgaa agccccaacg
     1561 cgggccgggc gacttcgtcc ccctgccgtc ggtcggtgcg gccgggacgc cggacgggac
     1621 gggttattcg gtgccgtacc ggttgatctt cctcgggctc ctcctcctgc tcggcgtgct
     1681 cctcgtggtg gtgccgatcg cgaagtccgc gtggcgccgc cggctcctgc gtcgctcgcg
     1741 ggacccgcgc gagcacgtcc tggccgcgta ccgggtgttc gacggcgagg ccgccgacct
     1801 cgggttgggc cgccgcgagg gggagaccct cgacgagcac cgcgcgcggc tggccgcgac
     1861 gatcgcgttc accgacgggt acctcgggcg gctgacgacc caggcgaccc gagccgcgta
     1921 cgccgcggac gccccgacac ccgaggaggc aaggtcggcg gtccaggacg cccacgcgtc
     1981 gatccgggag ctccgcaggg atgcgggtct gatccggcgg atcgtgggcg cctaccggcc
     2041 cggcgtctag gaggggctca tcggccggtc tgcgctcggg cggcggcctc gcggagcgcc
     2101 cggacgaaac ggtcgcgatc ctctcggaac tggccgacct tgc
//
LOCUS       DATBRK010000365         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2523904, whole genome shotgun sequence.
ACCESSION   DATBRK010000365 DATBRK010000000
VERSION     DATBRK010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2523904"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..687
                     /locus_tag="VIH70_10835"
     CDS             58..687
                     /locus_tag="VIH70_10835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY8116899.1"
                     /translation="MDTVVMVLGFVAEVIAWSLVARGRNVWTTMTPVLATMGVVALVV
                     GPIAWSPEVAPGVSLAAGLGLGVLLYVATRVAMVIMARWGTFRRHALEMYRKQGGLSL
                     GVALQLSIALSVPGEELFWRGLFQGQLASTLEGRTALAAVLSWIAFVLANLPSANLAI
                     AAGALVGGAVWVALAWWTGGVLASLACHIVWTGMMVAFPVLRAPADTIT"
     gene            684..1640
                     /locus_tag="VIH70_10840"
     CDS             684..1640
                     /locus_tag="VIH70_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8116900.1"
                     /translation="MSEVVPAPIRRILEEGAFCHVAALTPKGPHLTPLVFASAAGRVW
                     VTTSRGSVKARAWRRDPAVAGLVRSGDRAAAFTGTVTTYDLLETGTWGRSIAQGPLLA
                     LAGFRFTQKNARFFAGYAVDAHQVPLAWSPPGRVFAEIRLERTAILESGRVASAWGDW
                     GETLEGVERFRADRAGASPLAGVPEGIRDGLGESGEGALAVGTGEGPVVLPVRWVIDG
                     GVVYAAAPSETLALAAPLRAPAVALQVDLPSSWRARAMRGAMIRGHGEVGVVSAVDSG
                     GGSATRIARLAGVELSDAALVGIRPATVVWWRGWSSGTVRAT"
     gene            1637..2113
                     /locus_tag="VIH70_10845"
     CDS             1637..2113
                     /locus_tag="VIH70_10845"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8116901.1"
                     /translation="MTRVEFDVVVDAPPEAVWEVISDPRRLPHWDRHIVRVEGVPAGG
                     LREGMRYTTEMRFFAFRSTIDGEVLEWDPPRHSTIRLTGLLEATVTSTIEPLAGDRSL
                     LEHVVDYRLRGGPLGEIAARSLGRMGGAHLALRHGTLAQKRDIEVRYRADRATDGD"
     gene            complement(2110..>2346)
                     /locus_tag="VIH70_10850"
     CDS             complement(2110..>2346)
                     /locus_tag="VIH70_10850"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY8116902.1"
                     /translation="SGISFRTVLVAILAVILIVFAIANFNSVKVNLLLFDTRARLVTV
                     IVVAAGLGFVIGYFVGRPGRLQRKYLKQRDEGLD"
BASE COUNT          287 a          777 c          922 g          360 t
ORIGIN      
        1 gtgatcacgg cgctcaccgc ggagttcgcc gcgaaggtaa cgttcctgcc ctgagccatg
       61 gataccgtcg tgatggtcct cgggttcgtc gcggaggtca tcgcctggtc gctggtcgcc
      121 cgcgggcgga acgtgtggac cacgatgacg cccgtgctcg ccacgatggg cgtggtcgcg
      181 ctggtcgtgg gtccgatcgc ctggtcgccc gaggtggctc ccggcgtctc gctggcggcc
      241 gggctcgggc tcggcgtgct gctgtacgtg gcgacccgcg tcgcgatggt gatcatggcg
      301 cggtggggca cgttccggcg gcatgcgctc gagatgtacc gcaagcaggg cgggctctcg
      361 ctcggggtcg cgctccagct ctcgatcgcg ctgtccgtgc cgggggagga gctgttctgg
      421 cgcgggctct ttcagggcca gctcgcatcg acgctcgagg gtcgaaccgc gctcgcggcg
      481 gtgctctcgt ggatcgcgtt cgtgctcgcg aacctcccga gcgcgaacct cgcgatcgcg
      541 gccggtgcgc tggtcggcgg cgcggtctgg gtcgccctcg cgtggtggac gggcggcgtg
      601 ctggcgagcc tggcctgcca catcgtgtgg acgggcatga tggtcgcgtt ccccgtactg
      661 cgggcccccg ccgacacgat cacgtgagcg aggtcgtccc cgcgccgatc cgacggatcc
      721 tcgaggaggg cgcgttctgc cacgtcgcgg cgctcacgcc gaaggggccc cacctgaccc
      781 cgttggtgtt cgcctcggcc gccgggcggg tgtgggtgac cacgtcgagg gggtcggtca
      841 aggcgcgggc gtggcgacga gatccggcgg tggccgggct ggtccgctcc ggggaccggg
      901 ccgcggcctt caccgggacc gtcacgacct acgacctgct cgagacgggc acgtgggggc
      961 gctccatcgc gcagggaccg ctgctcgcgc tcgcggggtt ccgcttcacg cagaagaacg
     1021 cccggttctt cgcgggctac gcggtggacg cccaccaggt cccgctcgcc tggtcgccac
     1081 cgggacgagt cttcgcggag atccggctcg agcgcacggc gatcctcgag tccggccgcg
     1141 tggcgagcgc ctggggagac tggggcgaga cgctcgaggg tgtcgagcgc ttccgcgccg
     1201 atcgggccgg tgcgtcgccg ttggctggcg tgcccgaggg gatccgggac ggcctgggcg
     1261 agagcgggga gggcgccctc gcggtcggca cgggcgaggg gccggtcgtg ctcccggtgc
     1321 ggtgggtgat cgacggcggt gtggtgtacg ccgcggcgcc atcggagacg cttgccctcg
     1381 cggcgccgtt gcgtgctccc gcggtcgcgt tgcaggtcga cctgccgtcg tcgtggcgcg
     1441 cgcgggcgat gaggggggcc atgatccggg gccacgggga ggtcggcgtc gtgtccgcgg
     1501 tcgactcggg cggtggatcg gccacccgga tcgcgaggct ggcgggcgtc gagctctcgg
     1561 atgcggcgct ggtcgggatc cgacccgcga ccgtcgtgtg gtggcggggt tggtcgtccg
     1621 ggacggtgag ggccacgtga ccagggtcga gttcgacgtg gtcgtcgacg ccccgcccga
     1681 ggccgtctgg gaggtcatct cggatccccg gaggctgccc cactgggacc gacacatcgt
     1741 gcgcgtcgag ggggttccgg ctggcggcct ccgcgagggc atgcgctaca cgaccgagat
     1801 gcgcttcttc gcgttccgca gcacgatcga cggcgaggtc ctcgaatggg acccgccgag
     1861 acactcgacg atccggctca ccggattgct cgaggccacg gtcacctcga cgatcgagcc
     1921 gctcgcgggc gatcggagcc tgctcgaaca cgtcgtcgac taccggctcc ggggcggccc
     1981 actcggcgag atcgcggcgc ggagtctcgg gcggatgggc ggggcccacc tggcgctgcg
     2041 gcacgggacg ctggcgcaga agcgcgacat cgaggtgcgc taccgcgcgg accgggcgac
     2101 ggacggcgac tagtcgagac cctcgtcgcg ctgcttcagg tacttgcgct gcaggcgtcc
     2161 cggccggccc acgaagtagc cgatgacgaa cccgagcccg gcggcgacga cgatcaccgt
     2221 gaccaggcga gcgcgtgtat cgaacagcag caagttgacc ttgaccgaat tgaagttcgc
     2281 gatcgcgaag acgatcagga tgaccgcgag gatcgcgacg agcaccgtgc ggaacgagat
     2341 ccccga
//
LOCUS       DATBRK010000366         4474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1659068, whole genome shotgun sequence.
ACCESSION   DATBRK010000366 DATBRK010000000
VERSION     DATBRK010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659068"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VIH70_10855"
     CDS             <1..180
                     /locus_tag="VIH70_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8116903.1"
                     /translation="AGLWKSKYDWPYDVRDGVFRHPGSEGKVPVFEVAPTKAFAYDRG
                     GNDSATRYRFRLSSD"
     gene            673..1374
                     /locus_tag="VIH70_10860"
     CDS             673..1374
                     /locus_tag="VIH70_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007233078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8116904.1"
                     /translation="MTGQAPAAPAHPVAAPLVYSWSAGKDSAFGLWTLLREPAYEVRA
                     LLTTITETYDRVSISGVRESLLDAQAEMLNLPLIKVRIPPACPNDLYEHRMASALAGR
                     EFEGIDHVAFSDLFLEDVRAYREDRLARADKHCVFPLWHRDTPQLAQDIIAQGFRAIV
                     VCLDPRVMDPSFAGRAFDLALLADLPDDVDPCGENGEFHTFVWDAPMYQQLIPCTTGE
                     VVTRDGFVYCDVASR"
     gene            complement(1690..1911)
                     /locus_tag="VIH70_10865"
     CDS             complement(1690..1911)
                     /locus_tag="VIH70_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8116905.1"
                     /translation="MDMVTAYLDGVVSEDQARRINHHLDGCQGCRAALHQFRTVIRLA
                     GRLTPADAASIDPLIRDRLTATLRAPRRH"
     gene            complement(1949..3514)
                     /locus_tag="VIH70_10870"
     CDS             complement(1949..3514)
                     /locus_tag="VIH70_10870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8116906.1"
                     /translation="MRAERHLLNRRLGPLYAAVFFQNLALWVPIEKLFMTTIGFNAAS
                     VGVMASVYALVVPVLEVPSGVLADRWSRRGVLILAAIAAILSVLIGGLSQNVATYMIS
                     AGFLGVFFALQSGTLESVVYDTVLEETGSSDAFEKTMGRVRLVESVALVASALAGGAI
                     AAVAPLRATYLLTAPLLATSCVVLLRFREPRLHKAEDEESLRRQLGTTYRTILARGQL
                     RAVVALTVAGSVLMQGMLEFGPLWLVALSVPAFLYGPQWAGLTSALGLGALLGAQPWI
                     TRRWVVGLVAVAIVACCAVLALSHFALLVVAVQVLLTLLVVAVSIPVLRRLHDAVPSA
                     IRAGVASGVGTLTWLTFVPFALAFGAVSERAGVNEAGWLLVAIGIAAAVLIIVVLPRS
                     PAPRAEAAPLVPAPALEMEPIFPVDRFLPPDDPEWPGHWAYPPSSWDGLSLSVDGAEA
                     LEQARVAIAGMPPTLRRVIVLRDVQGAMPSVVRRSLDLRPEEERALLQQARGLVRLQL
                     EHYFEERESDDEG"
     gene            3654..3971
                     /locus_tag="VIH70_10875"
     CDS             3654..3971
                     /locus_tag="VIH70_10875"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8116907.1"
                     /translation="MVNDAFKALAHPIRRVIVERLSNGPATVGEATAGLGVSKPAISR
                     HVKVLEDAGVVMRRIQGRTHRLGLNPGPLAEAVDWIDHQRAVWERMFDAVDEYLEARK
                     ESP"
     gene            3968..4426
                     /locus_tag="VIH70_10880"
     CDS             3968..4426
                     /locus_tag="VIH70_10880"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8116908.1"
                     /translation="MTDARPPEGVVLRLERTFNAPAQAVFDAWTSPGVLRRWWPAGPD
                     WDTPVAEVDVRVGGKLRLVMRRPDGQAFGGGGEYLEIRPPERLVFTWRWDEDEGNEGL
                     QVVEIELNERPDGTTAVVLTNTGLRDEEARRTHEEGWQASFDNLDRVLAS"
BASE COUNT          811 a         1523 c         1433 g          707 t
ORIGIN      
        1 gccgggctct ggaaatcgaa gtacgactgg ccctacgacg tgcgtgacgg cgtcttccgc
       61 cacccaggaa gcgagggcaa ggtgccggtg ttcgaggtgg ctcccaccaa ggccttcgca
      121 tacgacaggg gcggcaatga cagcgcgacc cgctaccgat ttcgcctcag tagtgactag
      181 ctggaagcct accggtggtt ccatacgttg gccgcctcat cagccggccg ggattcgacc
      241 ggcagggtcg gactcgatcg accttcagcc cggcttcgga ctctgacccg tcacggcacg
      301 ccgggatggt cactggtcgc ggctaacctc cgcctggcgc gcggcgcaca tcctcacgac
      361 ctggaggagc acccgatgaa caaggggcga gaggaggatc cgtctggggc atcgagcacc
      421 gccagcgatg tcgcggtgac gagagcctta cctcatctaa cgaacgtcag cgtcgctctc
      481 agcatccggc cctccatgac ccgctcccgt gccatccctc gcccgtccag ctgaccggta
      541 cagtccgatc atgctcagag ccgggcccgg gcgggtggaa aggaccggga agggcgccct
      601 atctctcgct tcatcccagg cgccctccga cttgcccaga ccctcgtcct caccggctga
      661 ccgaggacgc ccatgaccgg tcaggcgcca gccgctccgg ctcatcccgt cgcagccccg
      721 ctcgtgtatt cctggagcgc cgggaaagac tccgcgttcg gactatggac gcttcttcgt
      781 gagcccgcct acgaggtgcg ggctctcctc accaccatca ccgagaccta cgatcgcgtc
      841 agcatctccg gcgtgcgcga atcgctcctg gacgcccagg ccgagatgct caacttgcca
      901 ctcatcaagg tccggatccc gcccgcctgc ccaaacgatc tctacgagca tcggatggcc
      961 tccgccctcg ccggtcgaga attcgagggg atcgatcatg tcgccttctc cgacctgttc
     1021 ctcgaagacg tccgtgccta tcgcgaagac cgcctcgccc gagccgacaa gcactgcgtc
     1081 ttcccactgt ggcaccgtga taccccccag ctcgctcagg acatcatcgc ccagggattc
     1141 cgagccatcg tcgtttgtct cgacccgcga gtcatggatc cctcgttcgc cggcagggcc
     1201 ttcgatctcg ccctcctcgc cgacctcccc gacgacgtgg acccatgcgg cgagaacggt
     1261 gagttccaca ccttcgtctg ggacgctccg atgtaccaac aactcatccc ctgcaccact
     1321 ggcgaagtcg tcactcgcga tggcttcgtc tactgcgacg tggcttcgcg atgacgcttt
     1381 ggtgggtgaa ggatcggccg gagctccctc aagcatcagg tcaggagccg cctcgccacc
     1441 ctggaaggcg tccgcaaatc ccgggactcg attggaacga gcactcagga tctgggcacc
     1501 gaatcgctga tctgggcacc gaatcgctgg cggcatcgcc atctcacatc gactcgtcgc
     1561 ccccgagtcg ctcgttcgga tcgtatccgg ccgccacgag ggaaccgacc gtggggaaca
     1621 gcggctgcgg aaggtcatcc cagtcgaacc tgggccaccg ccgggtctcc ggcttgggtc
     1681 ggagacgcct tagtgacgtc gaggtgctcg caaggttgcc gtaaggcgat cgcggatcag
     1741 cgggtcgatg cttgccgcat cagccggggt caggcgaccg gcgaggcgga tgaccgtccg
     1801 gaactggtgg agggctgccc ggcagccctg gcatccatcg agatggtggt tgatgcgtct
     1861 ggcctggtcc tcgctgacca cgccgtcgag gtaggcggtc accatgtcca cgacctcgat
     1921 gcaccgcaaa tcgtccctac gggaaccgct atccttcgtc atcgctttct ctctcctcga
     1981 agtaatgctc caattgcaag cgaacgaggc cccgagcctg ctgaagcaag gctcgctcct
     2041 cctcggggcg aagatccagc gatcgtcgca ccacactcgg catcgctcct tggacgtcgc
     2101 gaaggacgat cactcggcgg agggttggcg gcatccccgc gatggcgacg cgcgcttgtt
     2161 ccaaagcctc ggcaccgtcg acggagaggc taaggccgtc ccaggaggaa ggggggtacg
     2221 cccagtggcc tggccattcc ggatcgtccg ggggaaggaa ccgatcgacg gggaatatgg
     2281 gctccatctc cagtgccggg gcgggaacga ggggtgcggc ctccgctcgc ggagccgggg
     2341 agcgagggag cacgacgatg atcagcaccg cggccgcaat tccgatcgcc accaagagcc
     2401 agccggcctc gttcacgccg gcgcgctcgc tgaccgctcc gaacgcaagt gcgaatggaa
     2461 cgaacgtcaa ccaggtgagc gtgccgacgc ctgaagccac gccggcgcgg atcgctgacg
     2521 gcacggcatc gtgcagcctt cgcaataccg gaatgctgac cgcgacgacc agcaacgtca
     2581 gcaacacctg cacggccacg accagcaggg cgaaatgact cagggccagg acggcgcagc
     2641 atgcgacgat cgcgaccgcc acgagcccca ctacccatcg ccgcgtgatc cagggctgcg
     2701 ctcccaggag cgcgcccaac ccgagtgccg aggtgaggcc cgcccactgg ggaccgtaga
     2761 ggaaggctgg tacggagagg gcgacgagcc agagtgggcc gaactccagc atgccctgca
     2821 tcaggaccga tcccgccacg gtgagcgcca ccaccgcccg gagctgtccg cgagccagga
     2881 tcgtccggta cgtcgtcccc agctgtcggc gaagcgactc ctcgtcttcc gctttgtgca
     2941 accgtggctc ccggaatcga agcagcacca cacacgaagt cgccaacaac ggagcggtca
     3001 agaggtaggt cgcccgcagc ggcgcgacgg cggcgatcgc tccaccggcc agtgcgctcg
     3061 cgaccagcgc gacgctctcc acgagccgga cacgacccat cgtcttctcg aacgcgtcgc
     3121 tactccccgt ctcctcgagc accgtgtcgt acacgacgga ttcgagcgtg cccgactgca
     3181 gggcgaagaa cacgccgagg aagcccgcgg agatcatgta ggtcgcgacg ttctggctga
     3241 gaccaccgat caggacgctg aggatcgccg cgatcgccgc gaggatcagc acgccacgtc
     3301 gactccaccg atccgccagt acccccgatg gcacctctag caccggaacg acgagcgcgt
     3361 agacggatgc catgaccccg accgaggccg cgttgaagcc gatcgtggtc atgaacagct
     3421 tctcgatggg gacccacagg gccaggttct ggaagaagac tgccgcgtag aggggaccga
     3481 ggcgccggtt cagcagatgc ctctctgccc gcatcgaggt cgctggtgta gtcgccgtag
     3541 cctgctcctc ccagccctct cgtcgagccg acgtcacgag gggtcgacct catcggagtc
     3601 tccgtcggcc ggggccgtcg tcgcgatccc cctgtggtga taaagttagc ataatggtta
     3661 acgatgcctt caaggcgctc gcccacccca tccggcgtgt gatcgtggag cggttgtcca
     3721 acggtccggc aaccgtcggc gaggcgacgg ctggcctcgg ggtgtctaag ccggctatct
     3781 cgaggcacgt gaaggtgttg gaagacgccg gggtcgtgat gcgcaggatc cagggccgga
     3841 ctcaccgact gggcctcaac cccggccctc tggccgaagc ggtcgattgg atcgatcacc
     3901 agcgggcggt ctgggagcgc atgttcgacg cggttgacga gtacctcgaa gcgagaaagg
     3961 agagcccatg acggacgcga gaccgccgga gggagtcgtc cttcgactgg agcggacgtt
     4021 caacgctccc gcgcaggctg tcttcgacgc gtggactagt cccggggtgc tgaggcgctg
     4081 gtggcccgcc ggaccggact gggacacacc ggtcgccgag gtggatgttc gggttggggg
     4141 caagctacgg ctcgtcatgc gcaggccgga cggccaggcg ttcggcggtg gcggcgagta
     4201 cctcgagatc agaccgcccg agcgcctggt cttcacctgg agatgggatg aagacgaagg
     4261 gaacgagggc ctccaggtcg tcgagatcga gctcaacgag aggcccgacg gaaccacggc
     4321 cgtggtcctt acgaatactg gcctgaggga cgaggaggcc aggagaacgc atgaagaagg
     4381 ctggcaggcg tccttcgaca atctcgaccg cgtcctggcg tcgtgaactc tccgatcccc
     4441 agccgttttc cgtgcgctcg ttaccccgac gacg
//
LOCUS       DATBRK010000367         8638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2670128, whole genome shotgun sequence.
ACCESSION   DATBRK010000367 DATBRK010000000
VERSION     DATBRK010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670128"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VIH70_10885"
     CDS             complement(<1..193)
                     /locus_tag="VIH70_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116909.1"
                     /translation="MHGPPRADRAGGGAGGASGGGRGPMKVTGAQALFRSLEGEGVDL
                     AFGIPGGAILPAYDPLIDSK"
     gene            complement(118..459)
                     /locus_tag="VIH70_10890"
     CDS             complement(118..459)
                     /locus_tag="VIH70_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116910.1"
                     /translation="MVGMRYEFDRGGRTVANVLWEGPGQVTVDVTDAADRPQFDRFFG
                     EEVAYLDAGFDFGGGMDEAGMTTRRRDWTPWEFERATRNLAHRLGCTVRRVQTGPVEE
                     REERRAEVAAP"
     gene            775..1689
                     /locus_tag="VIH70_10895"
     CDS             775..1689
                     /locus_tag="VIH70_10895"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8116911.1"
                     /translation="MGPDPQLDAVIAAVPEWRGHEPEITPIEAGRTNRNYRVEVGSGT
                     FFLRLSHEDTALLGIDRAAEYEAALAAAAAGVGPEVVAHLPEHGCLITAWVPGEPLAE
                     GDMERGSVLTDVVRVVSTIHAGPALPTTFDAFRIVEEYRRVSEEHDVAIPHAYDPAHA
                     QAERIEAAFARAPVPPRPCHNDLLADNFLKGDDGFWLVDYEYSGMGDPFFDLGNLSIN
                     NGLSEASQERLLRLMFGEPTDAHRARLRLMRIMSDYREAMWGVIQQGLSTLDIDYVAY
                     ADRHFDRLSRSMADERFERWLDQAQEAI"
     gene            1691..4201
                     /locus_tag="VIH70_10900"
     CDS             1691..4201
                     /locus_tag="VIH70_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8116912.1"
                     /translation="MPTDAPTDAPTDAPTNAPTDAPTHAPINAKDDRARVVIVGGGVA
                     GTSIAYHLTKLGWHDVLLLDRSELTSGSTFHSAGLVAQLRNSVSHTRMMMDGVELYPR
                     LEEETGVDPGWHEVGTLHLASSPYRLEALARQAGWAKSFGLPVEIVSAREALERFPLL
                     DVSNVLGAQFVPTDGHLDPTGLTMAFAEGAKRGGARIRTGVRVHEVVVRDGRVTGVVT
                     DHGAIEAEVVVNAAGIWAHELGRLAGVEIPVVPMEHQYLITRPIDGVTAAFPTLRDPD
                     NLVYVRGEVGGLVIGGYERNPDPWHVDTPIPQDFNHRLLAEKWERFEPIAEGAFNLIP
                     ALRTTEINRFINGPEAFTPDDDFILGETEVRGFFVAAGFCAHGITGGAGVGRYTAEWI
                     VDGEPSMDLSRMDVRRFGAHYRSRRYALARAHEIYAKHYDVKYPGEDYRAGRPLKVSP
                     TYARLEALGAEFGEKAGWERANWFGSNEDLAYAPLRPRGWAGEHWSTAIAAEHVATRE
                     RAGLFDETSFAKIEVSGPHACAFLRRICANDVDVADGRVVYTQMLNRRGGIQCDLTVT
                     RLAPERFRIVTGTASGSLDLAWIARHLERGGGVEIRDVTSSLACLGLWGPRARDILSS
                     VCSDDLSNAAFPFMTSREITVGDAPCRALRVTYVGELGWELYTPSEFGASLWDTLFEA
                     GTPHGMVPAGYRAIDSLRLEKGYRAWGSDVTPDDSPLEAGLAFAVASDKDFLGKQAFE
                     RLHADGVRRRLACMVLTDPRSSTLGNEPVRAGGDVVARVTSGGIGYTLGASIAYAYLP
                     SELAATGTAVDVEVFGEWIAAEVADEPLYDPKGERIRA"
     gene            complement(4191..4550)
                     /locus_tag="VIH70_10905"
     CDS             complement(4191..4550)
                     /locus_tag="VIH70_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116913.1"
                     /translation="MKVVTTPRAAEYIREAGGSVWVWLDPHRGLVGSHVWLEAHCEPP
                     RSSRRTKFTRASRRPHRFRQLEQDGLVVHYDFGNMAAPEELHFDRKGWRRGTYRLEAY
                     WNGSVFVGDDVPPPVRP"
     gene            complement(4619..6241)
                     /locus_tag="VIH70_10910"
     CDS             complement(4619..6241)
                     /locus_tag="VIH70_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8116914.1"
                     /translation="MSMSDQAARDTADLEKFGYKQELNRGLGTFSSFAVAFSYISPST
                     GIFTLFGLGMAAMGAYLFWAWPVVALFQFVVALNFAELSSHFPVAGSVYQWTKYLAGR
                     GYAWFTGWFYLIAGILTVASVCATLPLALLPMLNTAFGWSLNTDLGSADQAVVAIVTL
                     VLITVLNIYGVRLVAIVNNTGVIFEILGMVVFAIILAIAHNDQGLGVIVNADKTGNIF
                     EFPAPFSISFFLVAMFMSLYVIYGFDTASTLAEETHNPRQEAPKAVLGSVAGAFVIGA
                     IFLWGLLIAVPNMSEAVAGFFGPAVIIDSVLSDTLTVIYLLVVSAAIFVCCMSILTST
                     IRLAFGMARDDQLPFSKTMAKVSPRLHTPVATCVIIGALSAIPFIQFSGPTVIAVGAT
                     ASIYLSYLLGNLAVMRARTKGWPKSRAPFSLGGWGKVVNVVAIVWGVAMLLNFLTPAS
                     VNSAFDPNASGANYLRIFANPKAIQTDWYVEGDQLVDFKVDFLNKIPIIWLVFAVVLI
                     VGAIYYFAVQRKKPYEAVAAPTDEDLEGIIPV"
     gene            complement(6270..7070)
                     /locus_tag="VIH70_10915"
     CDS             complement(6270..7070)
                     /locus_tag="VIH70_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY8116915.1"
                     /translation="MEQPLPAERHRRIHELLREERVVRVSALSERLGVSEVTIRRDLE
                     VLERRGMLERTHGGAVATQWVRSEPAYREAASTNPLEKRLIGATAAALVEPGDALYLN
                     GGTTTLQVFRHLRAPGLKVATNHVGIALEAADRDVELLLLGGHYRAPSNSVVGPFATE
                     ALRRTHATKAFVGVEGISVGSGLTTPVAAEAEIARVMIEQTRGRVLVVADHSKIGTVA
                     DFVIAPLEEVDALVVDEGCSEAYRQRLTEAGIEVIVAAERASAASGGR"
     gene            7540..8109
                     /locus_tag="VIH70_10920"
     CDS             7540..8109
                     /locus_tag="VIH70_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8116916.1"
                     /translation="MANLQVGILEDDEVLRRGIVAILSEQGCSCISLDAARDLSPGAA
                     DAKATLDVAVICSKSARDLELSCPVILLTSPKLSGQASRHVTPKVMATLPHDDLTGDQ
                     LVASVRAAAAGLRVEDANSGAASEPILDLRRIEVLRLLATGATTRAIARELHLSERTV
                     KTLVHDIQLSLGTTTRAQAAAEAVRLGII"
     gene            complement(8261..>8638)
                     /locus_tag="VIH70_10925"
     CDS             complement(8261..>8638)
                     /locus_tag="VIH70_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116917.1"
                     /translation="FSGLGSVPAIATLEVTGTVDYDCANPQGHLSPGQNPVAAQPGLA
                     QQDLGNADHNGRGTINDLTATATAPDPAPSAQEVGCGGGGSTQWTVVNPVVTVTGAHL
                     TITQNGVIVFCRNYTEDGTGTAC"
BASE COUNT         1432 a         2977 c         2901 g         1328 t
ORIGIN      
        1 ccttcgagtc gatcagcggg tcgtacgcgg gcaggatcgc cccgccgggg atcccgaacg
       61 cgaggtcgac gccctcaccc tcgagcgagc ggaacagcgc ctgcgcgccc gtcaccttca
      121 tggggccgcg acctccgccc gacgctcctc ccgctcctcc accggcccgg tctgcacgcg
      181 gcggaccgtg cagcccagcc ggtgtgccag gtttcgggtc gcccgctcga actcccacgg
      241 cgtccagtcg cgtcgacgcg tcgtcatgcc cgcctcgtcc atcccgccgc cgaaatcgaa
      301 cccggcgtcc aggtacgcga cctcctcccc gaagaaccgg tcgaactgcg gccggtccgc
      361 ggcgtcggtg acgtcgaccg tcacctgtcc cgggccctcc cacagcacgt tcgcgaccgt
      421 cctgcctcct cgatcgaact cgtagcgcat cccgaccacc cttctcctcg tccgtctcga
      481 tcgatgcgaa cccttggtgc cgaccggggt acaaaaagcc cctcgctggg cgaggggcat
      541 ctggcgcggg tcccgggcct gtctcggccc ggccgcgcct acgcgataag tacgagggct
      601 cgcggtccgg tcttctccgt gatggacatc gttgggccag tgtacggagg tgggttcccc
      661 tgtcaaccca ccggatgggt gacccgagca ggaacggtcg gccgacaggg gcgtagtcgt
      721 tcggaaccgc tcagccttga ccggaaacga tcaccccggc tagcgtctcg cgccgtgggc
      781 cccgaccccc agctcgatgc ggtgatcgcg gccgtgccgg agtggcgggg gcacgagccg
      841 gagatcaccc ccatcgaggc ggggcggacc aaccgcaact accgggtcga ggtgggttcg
      901 gggacgttct tcctccggct gtcgcacgag gacaccgcgc tgctcgggat cgaccgcgcg
      961 gccgagtacg aggcggcgct cgccgccgcc gccgccgggg tggggcccga ggtcgtcgcc
     1021 cacctgcccg aacacggatg cctgatcacg gcctgggtcc ccggggaacc gctcgccgag
     1081 ggcgacatgg aacgggggtc ggtactcacc gacgtcgtgc gcgtcgtctc gacgatccac
     1141 gcgggacccg cgctcccgac gacgttcgac gcgtttcgca tcgtcgagga gtaccggcgc
     1201 gtgtcggagg agcacgacgt cgcgatcccc cacgcctacg atccggcgca cgcccaggcg
     1261 gaacggatcg aggcggcgtt cgctcgcgca cccgtccccc cgcgtccgtg tcacaacgac
     1321 ctgctcgcag acaatttcct gaaaggggac gacgggttct ggctggtgga ctacgagtac
     1381 tcggggatgg gcgacccgtt cttcgacctg ggcaacctct cgatcaacaa cgggctctcg
     1441 gaggcgtcgc aggaacggct gctccggctg atgttcggcg agccgaccga cgcgcatcgc
     1501 gcccgcctgc ggctgatgcg gatcatgtcc gactaccgcg aggcgatgtg gggcgtgatc
     1561 cagcagggcc tgtcgacgct cgacatcgat tacgtcgcct acgcggacag gcacttcgac
     1621 cggctctcgc gttcgatggc ggacgaacgg ttcgagcggt ggctggacca ggcccaagag
     1681 gcgatctgac gtgccgaccg atgcgccgac cgatgcgccg accgatgcgc caacaaatgc
     1741 gccgaccgat gcgccgactc atgcgccaat taatgcgaag gacgatcgcg cccgggtcgt
     1801 gatcgtcgga ggcggcgtcg cggggaccag catcgcctac cacctgacga agctcggatg
     1861 gcacgacgtc ctgctgctcg accgctccga actgacgagc ggctccacgt tccactcggc
     1921 gggcctcgtc gcccagctcc ggaactccgt tagccacacg cggatgatga tggacggcgt
     1981 cgagctgtac ccgaggctcg aggaggagac cggcgtcgat cccggatggc acgaggtcgg
     2041 gacgctgcac ctcgcgtcct ccccgtaccg actcgaggcg ctggcccggc aggccggctg
     2101 ggccaagagc ttcggcctgc ccgtcgagat cgtctcggcc agggaggcgc tcgaacggtt
     2161 cccgttgctc gacgtctcga acgtgctggg ggcgcagttc gtcccgacgg acggccacct
     2221 ggatcccacc gggctgacga tggccttcgc cgagggcgcc aagcgcggtg gcgcgaggat
     2281 ccgcaccggc gtccgcgtgc acgaggtcgt ggttcgcgac ggccgggtga ccggcgtcgt
     2341 caccgaccac ggcgcgatcg aggccgaggt cgtcgtgaac gccgccggga tctgggccca
     2401 cgagctgggg cggctcgccg gtgtcgagat ccccgtggtg ccgatggagc accagtacct
     2461 catcacgagg ccgatcgacg gcgtcacggc ggccttcccg accctgcgcg accccgacaa
     2521 cctcgtgtac gtgcgagggg aggtcggcgg tctggtgatc ggcggctacg agcgcaaccc
     2581 ggacccctgg cacgtcgaca cgccgatccc gcaggacttc aaccaccgcc tgctcgcgga
     2641 gaagtgggag cggttcgagc cgatcgcgga gggtgcgttc aatctgatcc ccgccctgcg
     2701 caccaccgag atcaaccgct tcatcaacgg ccccgaggcg ttcacgccgg acgacgactt
     2761 catcctggga gagaccgagg tccgcggttt cttcgtggcg gcgggcttct gcgcccacgg
     2821 catcaccggg ggagccggcg tcgggcggta cacggccgaa tggatcgtcg acggtgagcc
     2881 gtcgatggat ctgtcgagga tggatgtgcg gcggttcggg gcgcactacc ggagccggag
     2941 gtacgcgctc gcgcgcgccc acgagatcta cgcgaagcac tacgacgtga agtacccggg
     3001 ggaggactac cgggcgggcc ggccgctcaa ggtctcgccg acctacgcgc gcctcgaggc
     3061 gctcggggcg gagttcggcg agaaggccgg ctgggagcgc gcgaactggt tcggttcgaa
     3121 cgaggatctc gcgtacgcgc cgctgcggcc gcgggggtgg gccggcgagc actggtcgac
     3181 cgcgatcgcg gccgagcacg tggcgacccg cgagcgcgcc gggctgttcg acgagaccag
     3241 cttcgccaag atcgaggtct ccggtccgca cgcgtgcgcg ttcctccggc gcatctgcgc
     3301 gaacgacgtc gacgtcgccg acgggcgcgt cgtctacacg cagatgctga accgccgggg
     3361 cggcatccag tgcgacctca cggtgaccag gctggcgccc gaacgcttcc ggatcgtgac
     3421 cgggacggct tcgggcagcc tcgacctcgc gtggatcgct cggcacctcg agcggggtgg
     3481 cggcgtcgag atccgcgacg tcacctcgag cctcgcgtgc ctcgggctgt gggggccccg
     3541 ggcgcgcgac atcctgtcgt cggtctgctc ggacgacctg tccaacgccg cgttcccctt
     3601 catgacctcc cgcgagatca ccgtcggcga cgcgccctgc cgcgcgctcc gcgtcaccta
     3661 cgtaggcgag ctcggctggg agctctacac gccctcggag ttcggcgcgt cgttgtggga
     3721 caccctgttc gaggccggca cgccacacgg gatggtcccg gccgggtatc gggcgatcga
     3781 ctccctccgc ctggagaagg gctaccgggc ctggggctcg gatgtgaccc ccgacgactc
     3841 gccgctcgag gccgggctcg cgttcgccgt cgcgtcggac aaggacttcc tcggcaagca
     3901 ggcgttcgag cgtctgcacg cagatggcgt ccggcgcagg ctcgcctgta tggtgctgac
     3961 cgacccgcgc tcctcgacgc tcggcaacga gccggtacgt gccgggggcg acgtcgtcgc
     4021 ccgcgtgacg tcgggcggga tcggctacac cctcggcgcc agcatcgcct acgcctacct
     4081 accgtcggag ctcgcggcga ccgggacggc tgtcgacgtg gaggtgttcg gcgagtggat
     4141 cgcggcggag gtcgccgacg agccgctgta cgaccccaag ggcgaacgca tcagggcctg
     4201 accgggggcg ggacgtcgtc gccgacgaag acggagccgt tccagtacgc ctcgagcctg
     4261 tacgttccgc ggcgccagcc cttgcgatcg aagtgcagct cctccggagc ggccatgttg
     4321 ccgaagtcgt agtggacgac gagcccgtcc tgctcgagct gtcggaagcg gtgtggccga
     4381 cgcgatgcgc gcgtgaactt cgtgcgtcgg ctggatcggg gcggttcgca gtgggcctcg
     4441 agccaaacgt gcgagccgac gagtccgcgg tgagggtcga gccagaccca cacggaacca
     4501 ccggcctcac ggatgtattc ggcggccctg ggcgtcgtca cgaccttcac ggtcacgatg
     4561 atggcacgag acgaacaagg gagggcggta tcggtccgcc ctcccttcga atgtcggctc
     4621 agaccgggat gatgccttcg aggtcttcgt cggtgggagc cgccaccgcc tcgtacggct
     4681 tcttgcgttg caccgcgaag tagtagatcg ctccgacgat gaggaccacc gcgaacacga
     4741 gccagatgat cgggatcttg ttcaggaagt cgaccttgaa gtcgacgagc tgatccccct
     4801 cgacgtacca gtcggtctgg atcgccttcg ggttggcgaa gatccgcagg tagttcgccc
     4861 cggaggcgtt cgggtcgaac gcgctgttca ccgaagccgg cgtgaggaag ttcaggagca
     4921 tcgccacgcc ccacacgatc gcgacgacgt ttacgacctt gccccatcct ccgagcgaga
     4981 agggcgccct gctcttcggc caacccttcg tccgagcccg catgaccgcg aggttcccga
     5041 gcaggtaact caggtagatg gacgcggtcg cgccgacggc gatgaccgtg ggccccgaga
     5101 actggatgaa ggggatcgcc gagagcgccc cgatgatcac gcaggtcgca accggcgtgt
     5161 gcaacctcgg gctgaccttg gccatcgtct tggagaacgg cagctggtcg tcgcgcgcca
     5221 tgccgaacgc caaccgaatc gtggatgtca ggatcgacat gcagcacacg aagatcgccg
     5281 ccgacacgac gagcaggtag atgaccgtca gcgtgtccga cagcacggaa tcgatgatga
     5341 ccgcgggtcc gaagaagcct gcgaccgcct cgctcatgtt cgggacggcg atgagcagcc
     5401 cccacaggaa gatggcgccg atcacgaagg cgcctgcgac cgaaccgagc acggccttgg
     5461 gcgcctcctg ccgcggattg tgggtctcct cggcgagcgt cgaggcagtg tcgaacccgt
     5521 agatcacgta caggctcatg aacatcgcca ccaggaagaa ggagatgctg aacggtgccg
     5581 ggaactcgaa gatgttgccc gtcttgtcgg cgttgacgat gacgccgagc ccctgatcgt
     5641 tatgggcgat cgccaggatg atcgcgaaca cgaccatccc gaggatctcg aagatcaccc
     5701 cggtgttgtt cacgatggcc acgagcctga cgccgtagat gttcaggacc gtgatgagca
     5761 cgagcgtgac gatcgcgacg acggcctgat cggcgctgcc gagatcggta ttcaggctcc
     5821 agccgaacgc cgtgttcagc atcggcagga gcgcgagcgg gagcgtcgcg cacacggagg
     5881 ccaccgtgag gatgcccgcg atcaggtaga accagccggt gaaccacgcg tagcctcgcc
     5941 ctgccaggta cttggtccac tgatagacgg agcccgcgac tgggaagtgg ctcgagagtt
     6001 cggcgaagtt gagagccacg acgaactgga acagtgcgac gaccggccat gcccagaaca
     6061 ggtaggcgcc catcgccgcc atcccgaggc cgaacagtgt gaagatgccc gtcgacggcg
     6121 agatgtagct gaacgccacc gcgaacgagc tgaacgtccc aagcccgcga ttcagctcct
     6181 gcttgtagcc gaacttctcg agatcggcgg tatccctcgc cgcctggtcg ctcatcgaca
     6241 tcggctcccc ccttctccac cgacccggat caccggccgc ccgaggcggc cgaagcccgt
     6301 tcggccgcaa cgatgacctc gatccccgct tcggtcaaac gctgccggta cgcttcggaa
     6361 caaccctcgt cgaccaccag ggcgtcgacc tcctcgagcg gcgcgatcac gaagtcggcc
     6421 accgtcccga tcttcgagtg gtcggcgacc acgagcaccc ggccccgggt ctgctcgatc
     6481 atcacccgcg cgatctcggc ctcggccgcg accggcgtgg tcaggcccga gccgacgctg
     6541 atgccctcga cgccgacgaa cgccttcgtc gcgtgggtcc gtcggagcgc ctcggtggcg
     6601 aacggtccga cgaccgagtt gctgggcgcc cggtagtggc cgccgagcag gagcagctcc
     6661 acatcccggt cggcggcctc gagcgcgatg cccacgtggt tcgtcgcgac cttcaggcca
     6721 ggcgcacgca ggtgccgaaa gacctggagc gtggtcgtac ccccgttcag gtacagggcg
     6781 tccccgggct cgacaagagc ggccgcggtg gccccgatca ggcgcttctc gagcgggttc
     6841 gtcgaggcgg cttcgcggta ggccggctcg gaccgaaccc actgggtggc gacagcgccg
     6901 ccgtgcgtgc gctcgagcat ccctcgtcgt tccagcacct cgaggtcgcg ccggatcgtt
     6961 acctctgaga cgccgagccg ctccgacagt gccgagacgc gcacgacccg ctcctcccgc
     7021 aggagctcgt ggatccggcg gtgccgttcc gccggaagcg gctgctccac gtcgtaccga
     7081 ccccttccgc tcggcgaccg gatcgagcga gtctgtcccc actggatccg cgtgcgcaac
     7141 tccagcgggc ccgataaccg aaacggttct cgcgcgccgg tccgaccggc cctgcccacg
     7201 gaccgaaccc ggtccaacgg tcgaggcaac caacacgagg cctcatatgg tcgaggagac
     7261 gagccttgcg actgtcccga ctgcggtcgg agaggaatgg gtcacaaggg cgcggatgcg
     7321 catcaagacc cgcatccgtg gacgctacct ggttggttcc cgccgatccg cgagagacgc
     7381 tcacggagca caagctcatc cgttcgacgt gtgacgagga tcacatccaa tggatgacaa
     7441 gaatcacatc caatggatga caagaatcac atccaacgaa cgccctacgg ttgctaccgt
     7501 acgtcggcgg atgtcctcct gaaaggaaga acaccgtacg tggcgaacct acaggtgggc
     7561 atcctggagg acgacgaggt actgcgtcgc ggaatcgtcg cgatcctcag cgaacagggc
     7621 tgttcgtgca tctctctcga tgctgcacga gacctgtcgc ctggcgcagc agacgccaaa
     7681 gccaccctgg acgtcgccgt gatctgctcg aaatccgcac gcgatctcga actctcgtgt
     7741 cccgtcatcc tgctcacctc acccaagctc tccggtcagg cgagtcgcca cgtcaccccg
     7801 aaggtgatgg caacgcttcc ccacgatgac ctaaccggtg accaactcgt cgcaagcgtc
     7861 agggccgccg cagccggtct gcgggtcgaa gacgccaact cgggcgcggc ctccgaaccg
     7921 atactggatc tccggcggat cgaggtcttg aggctcctgg cgaccggagc gaccacccgg
     7981 gcgatcgccc gggaacttca tttgtccgag cgaaccgtga agacgctcgt tcacgacatc
     8041 cagctctccc tcggcacgac cacgagggct caggccgccg cggaggccgt tcgcctcggc
     8101 atcatctgag ctgtccctcg gggtgccgaa cgtcccgcag tcaggcagcc gggaggtggg
     8161 agaggaccat ttctagacca gtgaggacca gaagcggcgc caacgaagaa cggcggcgat
     8221 cccgaagggg atcgccgccg tcccgtgcgc tgcgtgtctg ctagcaagcg gtgccggtgc
     8281 cgtcttcagt gtagttccgg cagaagacga tcactccgtt ctgtgtgatc gtgaggtgcg
     8341 cgcccgtaac ggtcacaacc ggattcacga ctgtccactg ggtgctccct ccacctccac
     8401 atccgacctc ctgtgccgag ggagccggat cgggggccgt cgccgttgca gtcagatcat
     8461 tgatcgtgcc gcggccgttg tggtcagcgt tgccgagatc ttgctgagcg agcccgggct
     8521 gcgccgccac aggattctgg ccgggggaca gatgaccctg cgggttcgcg caatcatagt
     8581 caaccgttcc cgtgacctcc agggtggcga tcgctgggac acttcccagt ccgctgaa
//
LOCUS       DATBRK010000368         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1369853, whole genome shotgun sequence.
ACCESSION   DATBRK010000368 DATBRK010000000
VERSION     DATBRK010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369853"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VIH70_10930"
     CDS             <1..736
                     /locus_tag="VIH70_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY8116918.1"
                     /translation="ASATPQIKAALRKLVRALPEPFRAEAEAAASAVVIDPASWDRTH
                     VPAPAHLDELQRAVVEGVQVRIVYAAPDRPETERTVHPLGLVAKASVWYLIAATDAGM
                     RTFRLSRVRAVTLTKDPVERPEGFDLQTHWRSVVAELDERRVTFRATARVEAEAIPWL
                     RSSLGNRLSVREENAGDLVEIEIRSWSARSLAAELAGFGRWVEVLHPIEVRQELARIG
                     DALRERYGERTPGATRSRGRPLSATP"
     gene            complement(663..2180)
                     /locus_tag="VIH70_10935"
     CDS             complement(663..2180)
                     /locus_tag="VIH70_10935"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY8116919.1"
                     /translation="MRSQRLLSVLLFTTSTLVFAPAAGTAPAAPATCGPDASAGTLGV
                     GDPYYPRFGNGGYDVQHYDLAIRYGPRTNHLRGVATITALTTQSLSCFSLDLVGLTVH
                     AITVNGDPATWSRTHHELMITPAVPLASGADLSVLVTYDGFPKGFVIPSLGAVSGFIR
                     TADGVIVAGEPEAATAWFPVNDHPSDRASYTFRITVPTGFKVVANGVRESTDAQGDWT
                     SYVWQAHDPMASYLATFGVGEWTMRFRTTSSGLPVIDAIDPDVLPRVRASVRREPEIL
                     DFLESRFGPYPFESVGGIFPDTGRLAFALETQTRPVYSRYFFPNGDSVIVHELAHQWF
                     GDLVAVDRWQHIWLNEGFATYAEWLWSGHEGFGTPGRTFEAIWRAIPGDSPFWHVVIG
                     DPGVDHLFDGAVYVRGAMTLQALRNRVGDDAFWSTLHLWTEQHAFGTGRTDGFIALAE
                     QVSGQDLSDLFDVWLFTPGRPPASAVTGSGALRASPKAVDRATAWLRAFARRTAP"
BASE COUNT          353 a          800 c          865 g          314 t
ORIGIN      
        1 ggcgtcggcg accccgcaga tcaaggcggc gctgcgcaag ctggtccgtg ccctgcccga
       61 gccgttccgc gccgaggccg aggcggcggc gtccgcggtg gtgatcgacc cggcgagctg
      121 ggaccgaacg cacgttccgg cacccgcgca cctcgacgag ttgcaacgag cggtcgtcga
      181 aggggtccag gtccggatcg tctatgcggc ccccgaccgg cccgagaccg agcggaccgt
      241 ccacccgctc ggcctggtgg cgaaggcgtc ggtctggtac ctgatcgcgg ccaccgacgc
      301 ggggatgcgc accttccgcc tgagccgggt gcgcgcggtg acgctcacga aggatcccgt
      361 ggaacggccg gagggcttcg acctccagac gcactggcga tcggtcgtcg ccgagctcga
      421 cgagcggagg gtgacgttcc gcgccacggc gcgcgtcgag gccgaggcga tcccgtggct
      481 gcggtcatcc ctcgggaacc gtctgtcggt gcgcgaggag aacgccggcg acctcgtcga
      541 gatcgagatc aggagctggt cggcccggag cctggcggcc gagctcgcgg ggttcggcag
      601 atgggtggag gtcctgcacc cgatcgaggt tcggcaggag ctcgcgcgca tcggcgacgc
      661 gctcagggag cggtacggcg agcgaacgcc cggagccacg cggtcgcgcg gtcgaccgct
      721 ttcggcgacg ccctgagggc gcccgagccc gtgaccgccg aggcgggcgg gcggccgggc
      781 gtgaacaacc acacgtcgaa caggtcgctc aggtcctgac cggagacctg ctcggccagc
      841 gcgatgaacc cgtcggtcct gcccgtgccg aacgcgtgct gctcggtcca caggtgcagg
      901 gtcgaccaga acgcgtcgtc gccgacccgg ttgcggagcg cttgcagcgt catcgcgccg
      961 cggacgtaca ccgccccgtc gaacaggtgg tcgacgccgg ggtccccgat cacgacgtgc
     1021 cagaaggggg agtcgcccgg gatcgcacgc cagatcgcct cgaacgtccg ccccggggtc
     1081 ccgaacccct cgtgcccgct ccagagccat tccgcgtacg tggcgaagcc ctcgttgagc
     1141 cagatgtgct gccagcggtc gacggccacc aggtcgccga accactggtg cgcgagctcg
     1201 tgcacgatga ccgaatcccc gttcgggaag aagtagcgcg agtaaacggg cctcgtctgc
     1261 gtctcgagcg cgaaggcgag ccggccggta tcggggaaga tcccgcccac cgactcgaac
     1321 gggtaggggc cgaaccggct ctcgaggaag tcgaggatct cgggttcgcg acgaacggag
     1381 gctcgcacgc gcggcaggac gtcggggtcg atcgcgtcga tcacgggcag gccgctcgac
     1441 gtcgtgcgga accgcatcgt ccattcgccc acgccgaacg tcgcgaggta gctcgccatc
     1501 gggtcgtgcg cctgccagac gtagctcgtc cagtccccct gggcgtccgt cgactcgcgc
     1561 accccgttgg cgaccacctt gaacccggtc ggcaccgtga tccggaaggt gtacgaggcg
     1621 cggtcgctgg gatggtcgtt gaccgggaac cacgcggtcg ccgcctcggg ttcccccgcg
     1681 acgatgaccc cgtcggcggt ccggatgaac ccggacacgg cgccgaggct cgggatgacg
     1741 aaccccttcg ggaacccgtc gtaggtcacc aggacggaca ggtccgcgcc ggaggcgagc
     1801 ggtacggccg gcgtgatcat cagttcgtga tgcgtccgcg accacgtcgc cgggtcgccg
     1861 ttcacggtga tcgcgtgcac ggtcagacct accagatcca ggctgaagca gctcaggctc
     1921 tgcgtggtca gggccgtgat cgtcgccacc ccgcggaggt ggttcgtccg gggcccgtat
     1981 cggatcgcga ggtcgtagtg ctgcacgtcg tacccgccgt tgccgaaccg cgggtagtac
     2041 gggtcgccga cgcccagcgt tccggcggac gcatcgggac cgcaggtggc cggcgcggcc
     2101 ggcgcggtcc cggccgccgg cgcgaacacc agcgtcgatg tcgtaaagag caggacggac
     2161 aggaggcgtt gcgagcgcat gcaggcctcc gatccctcga tcgcgggcgg acgcgacgca
     2221 acgtatcacc ctgtgggttc gtccaggggc tcggcggagc cggccggacc ggagggttac
     2281 gcggggtacc gcggcaggat cagcggcggg ctcggcggct gcgagaagtc ga
//
LOCUS       DATBRK010000369         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1945811, whole genome shotgun sequence.
ACCESSION   DATBRK010000369 DATBRK010000000
VERSION     DATBRK010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1945811"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VIH70_10940"
     CDS             <1..399
                     /locus_tag="VIH70_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8116920.1"
                     /translation="TLAIGAAVFFGLGLFAMGRLSEDIPIAWIIFPSRTIAVLLVTVP
                     LAVAGRLRMTREAAPLVLASGVLEVAGLVAFTIGARHGIAVAAVLGSQFAAIATVAAY
                     FLFRERLARVQLAGVAVIAVGVAVLTALQA"
     gene            434..1957
                     /locus_tag="VIH70_10945"
     CDS             434..1957
                     /locus_tag="VIH70_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8116921.1"
                     /translation="MPDAFADHPEPELIDLRDEAVSRPALERLGKAIWDEALRYLYED
                     AMARPLRTMPYREMRRTFYGPSGEPAPAPETAAKADEVLGEFRERVAPYVFNTQHPGS
                     YSYFTPPPLTMSIAGETLCQWVNQGVDVWVAGPVGALVEEEVTTWLRALIGFGEGSWA
                     VLTSGGVMANIMALTVARDIHLARVLGLEDPPRAGALEGTRVYASDQAHFSIARALDV
                     LGFPPETLRILPSDERFRLHAAPLADAVAEDRAAGLVPFAIAAVAGSTNTGSVDLTGE
                     LADLAERERLWLHVDAAYGGGAAFSQRIAPRVPDLERADSVTIDPHKWFFQAYDIGAL
                     VVKRRDDLRETFHRTPEYYRHPNPENAPLNWFEYSIEGTRRFRALKLWLSWKHLGTSG
                     FARLIEHNVDLAVYLVARCREAPDFEVVPDEPELSVVCFRHLPEGLEGAALDAYQDRL
                     QVALEASGEAWVSTTKIRGATYLRAGIVNYLSSEADIDRMLDALRRLSAQAALAPRT"
     gene            2088..>2383
                     /locus_tag="VIH70_10950"
     CDS             2088..>2383
                     /locus_tag="VIH70_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8116922.1"
                     /translation="MPRTGTTHVLADRYELGPVLGQGGMARVHQGLDRQLGRRVAIKV
                     LAPPFDRDGEFVERFQREARAAAGLSHPNIVAVFDSGSDDGTHFIVTELVEGETL"
BASE COUNT          312 a          873 c          847 g          351 t
ORIGIN      
        1 acgctggcga tcggcgcggc cgtgttcttc ggactgggcc tgttcgcgat gggccggctg
       61 tccgaggaca tcccgatcgc ctggatcatc ttcccgtcca ggacgatcgc cgtgctcctc
      121 gtcacggtcc cgctcgcggt cgccgggcgg ttgcgcatga cgcgcgaggc cgcgccgctg
      181 gtgctcgcct ccggcgtgct cgaggtcgcc ggtctggtcg cgttcacgat cggcgcgcgc
      241 cacgggatcg cggtcgcggc ggtgctcggg tcgcagttcg cggcgatcgc gaccgtcgcg
      301 gcgtacttcc tgttccggga gcggctggct cgcgtccaac tcgccggtgt tgcggtcatc
      361 gccgtcgggg tcgcggtcct gaccgccctc caggcgtagg ttcgcggcct cctgggctgc
      421 tagcctcccc gccgtgccgg acgccttcgc cgaccatccc gaacccgagc tcatcgacct
      481 gcgcgacgag gcggtctcgc gtccggcgct cgaacgcctc ggcaaggcga tctgggacga
      541 ggcgctccgc tacctgtacg aggatgcgat ggcgcggccg ctccgaacga tgccctaccg
      601 cgagatgcgc cggaccttct acggcccctc gggcgagccg gcgccggctc ccgagaccgc
      661 cgccaaggcc gacgaggtgc tcggcgagtt ccgagagcgc gtcgccccgt acgtcttcaa
      721 cacgcagcac ccgggctcgt acagctactt cacgccccca ccgctcacga tgtcgatcgc
      781 gggcgagacc ctctgccagt gggtgaacca gggcgtcgac gtctgggtcg ccggccccgt
      841 gggggcgctc gtcgaggagg aggtcacgac gtggctgcgg gcgctgatcg ggttcggcga
      901 gggctcgtgg gcggtgctca cgtcgggcgg cgtgatggcg aacatcatgg cgctcaccgt
      961 cgcgcgggac atccacctcg cacgggtcct cggcctcgag gatccgccgc gcgcgggcgc
     1021 gctcgagggc acgcgcgtgt acgcgagcga ccaggcgcac ttctcgatcg cccgagcgct
     1081 cgacgtgctg gggttcccgc ccgaaacgct gcgcatcctg ccgtcggacg agcggttccg
     1141 gctgcacgcc gcgccgctcg ccgacgcggt ggccgaggac cgcgcggccg ggctcgtgcc
     1201 gttcgcgatc gcggcggtcg ccggctcgac gaacaccggc agcgtcgacc taaccggtga
     1261 gctcgccgac ctcgccgaac gcgagcgtct ctggctccac gtcgacgcgg cgtacggcgg
     1321 cggcgcggcg ttctcgcagc gcatcgcgcc gcgcgtcccg gacctggagc gggccgacag
     1381 cgtcacgatc gacccgcaca agtggttctt ccaggcgtac gacatcggcg cgctcgtcgt
     1441 gaagcggcgc gacgacctgc gggagacgtt ccaccgcacc ccggagtact accgccaccc
     1501 gaaccccgag aacgcgccgc tcaactggtt cgagtactcg atcgagggca cccgccggtt
     1561 ccgggcgctg aagctctggc tctcgtggaa gcacctgggg acgtcggggt tcgcccggct
     1621 gatcgaacac aacgtcgacc tcgccgtgta cctcgtcgcg cggtgccgtg aggccccgga
     1681 cttcgaggtc gtcccggacg agcccgagct ctcggtcgtg tgcttccgcc atcttcccga
     1741 gggcctcgag ggcgccgcgc tcgacgcgta ccaggaccgg ctgcaggtcg cgctcgaggc
     1801 gagcggcgag gcgtgggtct ccacgacgaa gatccggggc gccacgtacc tgcgcgcggg
     1861 catcgtcaac tacctgtcga gcgaggccga catcgaccgg atgctcgatg cgctccggcg
     1921 cctgtccgcg caagcggccc tcgcgccccg aacgtagact ccgccggatg tcgaacggcc
     1981 aagaagtacc tcttccggtt cggggcgcac ggaggcacgg gcggggcgcc ggttgacccc
     2041 tgagacacca ggcgtacggg ccgggccccg tgggtaaacc caggtccatg ccacggaccg
     2101 ggacgacgca cgtcctcgcc gaccggtacg agctcggccc cgtgctcggc cagggcggga
     2161 tggcacgggt ccatcagggc ctcgaccggc agctcggtcg ccgggtggcg atcaaggtcc
     2221 tggcgccccc gttcgaccgc gacggtgaat tcgtcgagcg gttccagcgg gaggcccgcg
     2281 ccgccgccgg gctgagccac ccgaacatcg tcgccgtgtt cgacagcggc tcggacgacg
     2341 gcacccactt catcgtcacc gagctcgtcg agggagagac gct
//
LOCUS       DATBRK010000370         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1080082, whole genome shotgun sequence.
ACCESSION   DATBRK010000370 DATBRK010000000
VERSION     DATBRK010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080082"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VIH70_10955"
     CDS             <1..655
                     /locus_tag="VIH70_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8116923.1"
                     /translation="TVIDAYEAFINELDGDPIIMGHSLGGAITQVLLNRGLGSVGVGV
                     SAATVKGIRDLPWSTLRAAGPALNPFKRGDPVPISEKEFHYAFANTMTEEESKPLYER
                     YAVPAASDVLADIALANLHRNPPTEVDFAKQGRIPLLFIGFSEDHVVPPKATMHNERK
                     YHDSVSITEYHEFEGRPHFPGAPGWEEVADFAIDWAERHAGLTVAEHAAAKDPVSGG"
     gene            803..1726
                     /gene="mdh"
                     /locus_tag="VIH70_10960"
     CDS             803..1726
                     /gene="mdh"
                     /locus_tag="VIH70_10960"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY8116924.1"
                     /translation="MRVTVVGSGFVGQTTAMRLVERGFAEVVLIDIVEGLPQGLALDM
                     RQSAPVVGFEPDVIGTNNYADTAGSDVVVITAGFPREPGMSRMDLLGKNAGIVREVVE
                     RFVPGSPNAIIIVVTNPLDEMTFLASDVSGFPRERVMGMAGVLDSARLRAFIAEELGV
                     SPLVVEAMTLGSHGESMVPLPRHATVDGKPLTELVDGATLERLYAQTRDAGAEIVALL
                     KKGSAYYAPSASAARMVEAIAGRNDEPLPVCAWCTGQYGIDGVYVGVPVTLSGAGVSE
                     IVELDLNDDELAALRAAAEGIRAKCADLASL"
BASE COUNT          293 a          591 c          631 g          276 t
ORIGIN      
        1 gaccgtcatc gacgcgtacg aggcattcat caacgaactg gatggcgacc cgatcatcat
       61 gggtcactcc ctgggcggcg cgatcaccca ggtgctgttg aaccgcggtc tcggaagcgt
      121 tggcgtcggg gtgtccgcgg cgaccgtcaa ggggatccgc gacctgccgt ggtcgacctt
      181 gagggcagcc gggccggcgc tgaacccatt caagcgcggc gatcccgtgc cgatttccga
      241 gaaggagttc cactacgcct tcgcgaacac gatgaccgag gaggagtcca agccgctcta
      301 cgagcgctac gccgtgccgg ccgcctctga cgtgctcgcc gacatcgcgc tcgcgaacct
      361 gcatcgcaac ccaccgacgg aggtggactt cgccaaacag ggtcgtatcc ccctgctgtt
      421 catcgggttc agcgaggacc acgtcgtccc tccgaaggcc acgatgcaca acgaacgcaa
      481 gtaccacgat tccgtatcga tcaccgaata ccacgagttc gagggtcggc cgcacttccc
      541 gggagctcct ggctgggaag aggtcgcgga cttcgcgatc gactgggcgg agcgacacgc
      601 gggactcacg gtggccgagc acgcagcggc gaaggatccc gtctccgggg gatgaccggt
      661 gacatgagga atcggagtcg ctccgagtcg ccgctatgaa tgaacacgcg ttccgctcgc
      721 aaccggccgt gtagcggaac ggcccgcggg gtcgggagcc tcggcgatag gatttggtct
      781 ccccgaggcg aggagtccat ccatgcgtgt caccgtcgtc ggaagcgggt tcgtcggcca
      841 gaccaccgcg atgcggctgg tcgaacgcgg cttcgcggag gtcgtgctga tcgacatcgt
      901 cgagggcctc cctcaggggc ttgcgctcga catgcgtcag tccgcccccg tcgtcgggtt
      961 cgagccggat gtgatcggca ccaacaacta cgcggacacg gccgggtccg acgtcgtggt
     1021 catcacggcc ggcttcccgc gagagccggg gatgagccgc atggatctgc tcggcaagaa
     1081 tgccggcatc gtgcgcgagg tggtcgagcg cttcgtcccc ggctcgccga acgcgatcat
     1141 catcgtcgtg acgaatccgc tcgacgagat gacgttcctc gcgtccgacg tctccggatt
     1201 tcccagggaa cgggtcatgg ggatggcggg agtgctcgac tcggcgcgcc tgcgggcgtt
     1261 catcgcggag gagctcggcg tgtcgccgct cgtggtggag gcgatgacgc tcgggtcgca
     1321 cggcgagtcg atggtgccgt tgccccgcca cgcgaccgtc gacgggaagc cgctcaccga
     1381 gctggtcgac ggcgcgacgc tcgagcgcct gtacgcgcag acgcgcgacg ccggcgccga
     1441 gatcgtggcg ctactgaaga aggggagcgc gtactacgcc ccgtcggcct cggccgcacg
     1501 gatggtcgag gcgatcgccg gcaggaacga cgagccgctg cccgtctgcg cgtggtgcac
     1561 gggccagtac gggatcgacg gggtctacgt cggcgtgccc gtgacgctct cgggagcggg
     1621 cgtgtccgag atcgtcgagc tcgacctgaa cgacgacgag ctggcggcgc tccgagcggc
     1681 ggccgaaggt atccgcgcga agtgcgcgga cctcgcgtcg ctctagcgca ctcgcccgtc
     1741 ggggggtcgc tcgcgacgca cccaccggag cccgaggacg ccgcccgcaa t
//
LOCUS       DATBRK010000371         5074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_216324, whole genome shotgun sequence.
ACCESSION   DATBRK010000371 DATBRK010000000
VERSION     DATBRK010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216324"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /gene="lepB"
                     /locus_tag="VIH70_10965"
     CDS             complement(<1..425)
                     /gene="lepB"
                     /locus_tag="VIH70_10965"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8116925.1"
                     /translation="MTEGPSGSEEPAGGVTLSEPRPAEQEERGSFLRELPVLFVVALA
                     VAIVLKTLVVQAFFIPSSSMEPTLEPGDRVLVQKVVYGPDRGDVIVFSDPQGRPGPDR
                     GIVGGFVHWLSSTLGIERPEHEDFIKRVIGLPGETVELR"
     gene            complement(708..1046)
                     /gene="rplS"
                     /locus_tag="VIH70_10970"
                     /pseudo
     CDS             complement(708..1046)
                     /gene="rplS"
                     /locus_tag="VIH70_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(1087..1761)
                     /gene="trmD"
                     /locus_tag="VIH70_10975"
     CDS             complement(1087..1761)
                     /gene="trmD"
                     /locus_tag="VIH70_10975"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY8116926.1"
                     /translation="MRIDVVTIFPGIFESPLRESLLGRAIGAGLLDVRVHDLRDSTSD
                     KHRQVDDESYGGGPGMVLKPEPVFAAVEALGTGERRTLLLSPAGRRLDQALARELASE
                     AWLVLICGRYEGVDERVVEGLPAEEVSIGDYVVAGGEVPALVVLEAVTRLVPGVIGRE
                     ESHEQDSFGDEGVLDHPHYTRPAEFRGMRVPETLLSGNHAEIERWRREAALEKTRRNR
                     PDLLER"
     gene            complement(1812..2321)
                     /gene="rimM"
                     /locus_tag="VIH70_10980"
     CDS             complement(1812..2321)
                     /gene="rimM"
                     /locus_tag="VIH70_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY8116927.1"
                     /translation="MDEPTVVVGRITRAHGVTGEVAVQVISEVSGRFDDGATVWLEDG
                     RTLTVESSRPHKDRLLVRFRGVKDRAQAEALQKALLLVPESASPRLPEGSWWDHVIVG
                     CAVQTDTGRALGTIRDVIHTAANDVWSAVDGEGVETLVPVLRDVILDVDTDAKKVVVR
                     EIPGLTAPG"
     gene            complement(2351..2668)
                     /locus_tag="VIH70_10985"
     CDS             complement(2351..2668)
                     /locus_tag="VIH70_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY8116928.1"
                     /translation="MAEDAPDNGPENGPENMPENMPENMPENMKDLLDYLARELVDDP
                     DAVRVEQVDDERGVLLTLRVAQDDMGKVIGRGGRTARAIRIVMKAAGMRAGIHHTGVE
                     IVD"
     gene            complement(2673..2963)
                     /gene="rpsP"
                     /locus_tag="VIH70_10990"
     CDS             complement(2673..2963)
                     /gene="rpsP"
                     /locus_tag="VIH70_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY8116929.1"
                     /translation="MSVRIRLKRLGAKKRPFYRVVVADQRSPRDGRFIENIGRYQPMD
                     DPSVIEIDEERALHWLRVGAQPSNTVRVLMTKVGIWERFASEKKDAKAARAS"
     gene            complement(2991..4331)
                     /gene="ffh"
                     /locus_tag="VIH70_10995"
     CDS             complement(2991..4331)
                     /gene="ffh"
                     /locus_tag="VIH70_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY8116930.1"
                     /translation="MFDTLTGRLDGIFKKLRSRGKLHPNQVDGALAEMRVALLEADVS
                     AEVADALLARVRDRALSEEVMKSLTPAQQVIKVVRDELQSTMGGTQVAFTLPSVRPAV
                     VMMAGVQGSGKTTACAKIALHLKSKGKRPLLVAADLWRPAAVEQLFTLGREIGVDVVS
                     DGKDAVRVAKAGVKHAGREGYDVAIADTAGRLHVDEEMMREARRVKDAIDPHHVVMAC
                     DAMTGQDAVIQARAFMREVDTTGFVLTKIDGDARGGAALSITAVTGRPVFFAGTGERP
                     EDLEPFYPDRMASRIMGMGDVLSLIDKAQDTLDREQAAETAEKMLSARFTLEDFLVQM
                     QALRKLGPLQDLLAMLPGVPGGKNALKDLEVDEGQLGRAQAIIQSMTREERQNPAIIG
                     GSRRSRIARGSGVTTAEVNALLKEFEQARKMMKSVLGGKGLPRVPGMPNLPGIG"
     gene            complement(4344..4739)
                     /locus_tag="VIH70_11000"
     CDS             complement(4344..4739)
                     /locus_tag="VIH70_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8116931.1"
                     /translation="MARAEILIVEDHPTMREAMRLILENAGFDTREASDGKTALSMAR
                     ERPPDVMFLDLNIPGTGGADVLAELKGDEATRNVRVIIITATGEEGREFVLSLGADEY
                     FTKPFPPTELLRTVERVLEGPPETSPAGS"
     gene            complement(4743..>5074)
                     /locus_tag="VIH70_11005"
     CDS             complement(4743..>5074)
                     /locus_tag="VIH70_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY8116932.1"
                     /translation="LHTKQPLMEELKKVRRVIEKAAGRPPDETLLVLDATTGQNGIAQ
                     ARAFAEAVEVTGVALTKLDGTAKGGVVLAVRDQLGVPVKVVGTGEGVGDLEPFDSEAF
                     AARLLGG"
BASE COUNT          713 a         1871 c         1674 g          816 t
ORIGIN      
        1 tcccgcagct cgaccgtctc gccggggagc ccgatcaccc gcttgatgaa gtcctcgtgc
       61 tcgggccgtt cgatgccgag cgtgctcgac aaccagtgga cgaacccgcc gacgatcccg
      121 cgatccgggc cggggcgccc ctgcggatcc gagaacacga tgacgtcgcc gcggtcggga
      181 ccgtacacga ccttctggac gaggacgcgg tcccccggct cgagcgtggg ctccatcgag
      241 ctcgagggga tgaagaaggc ctggacgacg agggtcttga ggacgatcgc gacggccagc
      301 gcgacgacga acaggaccgg gagctcgcgc aggaacgacc cgcgttcctc ctgctcggcg
      361 ggccgaggtt ccgagagcgt gaccccgccg gcgggctcct cggagcccga cgggccctcg
      421 gtcaccgact agccctggat ctctgtctcg gaggtctcgg tctgggccac cacgttcgat
      481 gcctcggccg cgtttgcctc ctgcgccgag ccgtcggccg ccggagcgtc gacggcgtcg
      541 gtctcggcgt ccggggcttc cgtctcgggc gcgccgacgt cggcggtctc cgacgaatcg
      601 gcctcatcgt cggctccatc ggtggagccg tcaccggcgg cggcgctggc ggccgcggcg
      661 cgctcggcct cggccgccgc gatcgccgcg agctcctcgt cgccgagccg gcgctcgcgg
      721 atccgtgcct tcttgccgcg acgctcgcgg aggtagtaga gcttggcccg gcgtacgtga
      781 ccgcgcgaga cgacctcgag cttgccgatc gacggcgagt gcagcgggaa cgtccgctca
      841 acgcccacgc cgaacgagat cttgcggacc gtgaacgtct cctggaggcc gccgccttga
      901 cgccggatca cgacgccctg gaagacctgg atgcgctcgc ggcccgcctc gaccacccga
      961 acgtgcacct tcacggtgtc gcccggcctg aactcgggca ggtccgtgcg gagatagggg
     1021 gcgtcgacga gatcggtctt gttcatggcg ggacgctcat cgtaacaggc gccacggagc
     1081 gccaggtcag cgttcgagca ggtcgggccg gtttcggcgg gtcttctcga gagcggcctc
     1141 gcggcgccag cgctcgatct ccgcgtgatt gcccgagagc agcgtctccg ggacccgcat
     1201 cccgcggaac tcggccggcc gcgtgtagtg ggggtggtca aggacgccct cgtcgccgaa
     1261 cgaatcctgc tcgtgggact cctcgcggcc gatgaccccg ggcacgagcc gcgtcaccgc
     1321 ctcgagcacc accagggccg ggacctcccc gcccgcgacg acgtagtcgc cgatcgagac
     1381 ctcctcggcc ggcaagccct cgacgacgcg ctcgtcgacc ccttcgtagc gcccgcagat
     1441 cagcaccagc caggcttcgc tcgcgagctc gcgcgcgagc gcctgatcca gcctgcgtcc
     1501 ggccggcgac agcaggagcg tgcgtcgttc gcccgtaccg agcgcctcga ccgcggcgaa
     1561 caccggctcg ggcttcagca ccatccccgg tccgccgccg tacgactcgt cgtccacctg
     1621 gcgatgcttg tcggacgtgc tgtcacgtag gtcgtgcacg cgcacgtcca ggaggccggc
     1681 cccgatcgcc cggccgagca ggctctcgcg cagcgggctc tcgaagatgc cgggaaagat
     1741 cgtgacgacg tcgatccgca cggccgcagg ctagcgcgac cgaggccgtg cgcgacgcgg
     1801 ggtggcgatg ctcaccccgg cgccgtcagc cccgggatct cgcggacgac gaccttcttc
     1861 gcgtccgtgt ccacgtcgag gatcacatcc ctgagcacag ggacgagcgt ctcgacgccc
     1921 tcgccgtcga ccgcggacca gacgtcgttc gccgcggtgt ggatcacgtc gcggatggtc
     1981 ccgagcgcgc ggcccgtgtc agtctggacg gcgcaaccga cgatcacgtg gtcccaccac
     2041 gagccctcgg ggagccgggg agacgccgac tcggggacga gcagcagggc cttctggagc
     2101 gcctcggcct gggcgcgatc cttgacgcct cgaaaccgaa ccaggagtcg atccttgtgg
     2161 ggccgggacg actcgacggt cagcgttcgg ccgtcctcga gccacaccgt cgccccgtcg
     2221 tcgaaccgcc ctgagacctc ggagatcacc tggacggcga cctcaccggt gacgccgtgc
     2281 gcccgtgtga tccggccgac cacgacggtc ggctcgtcca tcatccgctc ctcgccgttc
     2341 gcgggggcgg tcagtcgacg atctcgacgc cggtgtgatg gatccccgcg cgcatcccgg
     2401 cggccttcat cacgatcctg atcgcgcgag ccgtgcggcc gccgcgaccg atgaccttgc
     2461 ccatgtcgtc ctgcgcgacc cggagggtga gcagcacacc gcgttcgtcg tcgacctgct
     2521 cgacccggac ggcgtccgga tcgtcgacca actcgcgcgc gaggtagtcg agcaggtcct
     2581 tcatgttctc cggcatgttc tccggcatgt tctccggcat gttctctggc ccgttctctg
     2641 ggccgttgtc tggcgcgtcc tctgccaagg gctcaggagg ctcgcgcggc cttcgcgtcc
     2701 ttcttctcgg acgcgaagcg ctcccagatg ccgaccttgg tcatcaacac cctcaccgtg
     2761 ttcgagggct gcgcgccgac ccggagccaa tgcagcgcac gctcctcatc gatctcgatc
     2821 accgaggggt cgtccatcgg ctgatacctg ccgatgttct cgatgaacct gccgtcgcgg
     2881 gggctccgct ggtcggcgac gacgacccgg tagaaggggc gcttcttggc ccccaaccgc
     2941 ttcaacctga tacgaaccga catgttccct gactcctcac ggatggatcc ctacccgata
     3001 cccgggaggt tcggcatccc cgggacccgg ggcaggcctt tgccgcccag cacgctcttc
     3061 atcatcttcc tcgcctgctc gaactccttg agcagcgcgt tgacctccgc ggtcgtcacg
     3121 cccgatccgc gcgcgatccg cgagcgccga gacccgccga tgatagcggg attctgccgc
     3181 tcttccctgg tcatcgattg gatgatggcc tgcgcccggc cgagctggcc ctcgtcgacc
     3241 tcgaggtcct tgagcgcgtt cttgcccccg gggacgcccg gcagcatcgc gaggagatcc
     3301 tgcaaggggc cgagtttgcg gagcgcctgc atctggacga ggaagtcctc gagcgtgaac
     3361 cgggccgaga gcatcttctc cgcggtctcg gcggcctgct cccgatccaa agtgtcctgc
     3421 gccttgtcga tcaaggacag cacgtcgccc atccccatga tccgcgacgc catccggtcg
     3481 gggtagaacg gctcgaggtc ctcgggccgc tcccccgtcc ccgcgaagaa gactggcctg
     3541 cccgtcaccg ccgtgatcga gagcgccgcg ccaccgcgcg cgtccccgtc gatcttcgtg
     3601 agcacgaacc cggtggtgtc cacctcgcgc atgaaggcgc gagcctggat caccgcgtcc
     3661 tgaccggtca tcgcgtcgca cgccatgacg acgtgatgag gatcgatcgc gtccttcacg
     3721 cgccgggcct cgcgcatcat ctcctcgtcg acgtgcaggc gcccggccgt gtcggcgatc
     3781 gcgacgtcgt agccctcgcg accggcgtgc ttgaccccgg ccttcgcgac cctgaccgcg
     3841 tccttgccgt ccgagaccac gtccacgccg atctcgcggc cgagggtgaa cagctgttcc
     3901 acggcggccg gacgccacag gtcggcggcg acgagcagcg ggcgcttgcc cttggacttg
     3961 aggtggagcg cgatcttcgc gcaggcggtc gtctttcccg agccctgcac gccagccatc
     4021 atcacgacgg ctggtctgac ggacggcaag gtgaacgcca cctgggtgcc acccatcgtg
     4081 ctctggagct cgtcgcgtac gaccttgatc acctgctggg ccggcgtgag gctcttcatg
     4141 acctcctcgg acagcgcgcg atcgcggaca cgggccagca gggcgtcggc gacctcggcg
     4201 gaaacgtcgg cctcgagcag cgccacgcgc atctccgcga gcgcgccatc cacctggttt
     4261 gggtggagct tgccgcggga gcgcagcttc ttgaagatgc cgtcgagacg gccggtgagg
     4321 gtgtcgaaca tcgaggacct aggttacgac ccggccgggg aggtctcggg aggtccttcg
     4381 agcacccgtt cgacggttcg gagcaactcg gtgggtggga agggcttggt gaagtactcg
     4441 tcggcgccga gcgacaggac gaactcacgt ccttcctcgc cggtcgcggt gatgatgatc
     4501 acgcggacgt tgcgggtcgc ctcgtcgccc ttgagctcgg cgagcacgtc cgcgccgccc
     4561 gtcccgggga tgttgaggtc gaggaacatg acgtccggcg gccgttcgcg cgccatcgac
     4621 agcgccgtct tgccgtcgga cgcctcgcgc gtatcgaacc ccgcgttctc caggatcagc
     4681 cgcatcgcct cgcgcatcgt cgggtgatcc tcgacgatca ggatctccgc ccgcgccacg
     4741 tctcagcctc ccaggaggcg ggccgcgaac gcctcgctgt cgaagggctc cagatccccg
     4801 acgccctcac cggtgccgac gaccttgacc ggcaccccga gctggtcccg caccgccagg
     4861 acgactcccc ccttcgccgt gccgtcgagc ttcgtcagcg ccacccccgt cacctccacc
     4921 gcctcggcga aggcccgggc ctgcgcgatc ccattctgac ccgtcgtggc gtccagcacg
     4981 agcagcgtct cgtcgggcgg tcgccctgcg gccttctcga tcacccggcg gaccttcttc
     5041 agctcctcca tcaggggctg cttcgtgtgc agcc
//
LOCUS       DATBRK010000372         6928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2093048, whole genome shotgun sequence.
ACCESSION   DATBRK010000372 DATBRK010000000
VERSION     DATBRK010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093048"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VIH70_11010"
     CDS             <1..229
                     /locus_tag="VIH70_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116933.1"
                     /translation="NLRGERKGIDFGSQIRSYVLAPYRLVKDHRTNVEIGDVDRVLDG
                     DLEELIEAELRRRAEVGENPKRNGRLLGPSQ"
     gene            468..2144
                     /locus_tag="VIH70_11015"
     CDS             468..2144
                     /locus_tag="VIH70_11015"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY8116934.1"
                     /translation="MLDGQVAVQRGSGAFETGQDGASLHEGDTVRTGPDGRASIDYFD
                     GSLTRLDFDTSFTLVTLETLRDTAASIIESSQAEGNSYHRVPELTDAQSRFEVQTPTA
                     TASVHGTGYALLVDEGSTTIAVVEGVVTAGGSTGSVEIPAGTMAVVGTDGVVGPIRAI
                     PSELLDSDWLKFNRCPDHDAPACAEGDGGGPEGDGGGPEGPTEPPEGSGQGEQGSVPP
                     TSTGGAGGEGTTGAGGQSGTSGGGAGGTTPPRNQPPQAGFSATPRLGPAPLRVGFSYA
                     SHDPDGDPLSRHWSFGDGSAQSGGQTPSHTYDDPGRYTVTLTVSDPKGKRDATSKVIR
                     VASAEATLDHIIISPSNATIRPGDSRSYTAEAFDTDGGSMGDVTVDTDFSIAPKGSCK
                     DNTCTAKRPGAHTVTGTYHGESDQAKLTVEEAPPPPPSCPAYALAFHARPPANQSAGH
                     QFNAQIRVDVLGGSSDGPLSIDLSLQGGTFSGGETSATWTGQGVVNFNHLTIDGPGSY
                     AISASASCATSPDPAPVTITDGSNGSGKKGGSAIGLVLALPWMRRLRRAP"
     gene            complement(2047..4566)
                     /locus_tag="VIH70_11020"
     CDS             complement(2047..4566)
                     /locus_tag="VIH70_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116935.1"
                     /translation="METATRPPRDPRRADEPPAPSTARRVLNAVAGPTLIVGLVLIAL
                     RGIAFLPNLSDQHPDVLSFWLPRSCMLGRAIADGHVPLWNPFEMSGTWFAADPQSGWL
                     SLPMMASSWVFGCGGGLRALIVLNPILAGLGLFWFLRKEGLGRIAATAGGLSLSMAVS
                     ASILAVSLPFAGTLAWTPFILVGASGFFARAGWRRLAWLALAAFAWGQVATSHLSHGL
                     VMATGLVVAYVVARAIRETRRGTLHPGAAVGWSVAFLAFLPLANLAIVVPHFAVLARS
                     SLADGYGAIEGTVARARATTEDVPIPATGIWGGWPLALASTPGGYVGAAVLLFVPFAV
                     RDSARRYLVVALGAVAALAYLLTITLLVGAGCFRSFALSLPFGDVYLHNPSRLRYLAF
                     LIVPALGAIGVQWVMDQRPAFGEAMRWIGAGLAVLLVFPLLMGARVGRLTIFAIASVA
                     VIGVVWALAHRRRWAPVALCAILAVELLAGAIWSSMYRGGTVYLGLETGLHAALVAAP
                     LRWPDVDVHEYLSPGPIARALQRSGASDGRYLAWIRPDAYFNKGYLFTRKEPDWPALL
                     IGRSVLFEIPDTIGYSPVQPPGYWDYVHATNRFPVFYNAAVLQVPTLTDLRLLGARYL
                     IVPQGIPRPLPPGVSGRTIETEGAYRLVEIDDAEPRASVVPSWTVVSGERAALDAALE
                     PGFDPAAVAVLETDPGSTPGAAGIGGTAAYLEVAPEDVRIEAEASVPSILLVRNVWDE
                     GWSATLDGRSVPVLRTDGFLQGIALPPGHHDVRLTYREPAIGTGLALSAIAWLGFAVA
                     LVFVIVRGRLRARDGAGASMGAPAPDRSPNPPSSRSRSSRR"
     gene            4660..5337
                     /gene="ftsE"
                     /locus_tag="VIH70_11025"
     CDS             4660..5337
                     /gene="ftsE"
                     /locus_tag="VIH70_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY8116936.1"
                     /translation="MISLQHVTKVYRNGTTALEDVTVEVEKGEFVFIVGQSGSGKSTF
                     IRLLLKEEEASKGDIYVAGKNLAKLTTWKVPHLRRNIGTVFQDFKLLQDKTVFENVAF
                     ALEVIGKPKHVIDQRVPEILELVGLGDKLNNFPDELSGGEQQRVSIARACVNRPLVLL
                     ADEPTGNLDPSTSVEIMRLLDKVNRIGTTVVMATHDQAIVDAMRRRVIELDKGKVVRD
                     QARGVYS"
     gene            5337..6212
                     /gene="ftsX"
                     /locus_tag="VIH70_11030"
     CDS             5337..6212
                     /gene="ftsX"
                     /locus_tag="VIH70_11030"
                     /inference="COORDINATES: protein motif:HMM:NF038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY8116937.1"
                     /translation="MPLVEYLFRETTSGLRRNGLVAFAAISTTFIALFLFGLSLLISR
                     QVSLMIEATTGNVEVAVYLTDPVNPSTVSSLTSTLTDLPVVANVDYEDKQEACDRFRT
                     LFAEQKALVNNVDCDALPASLRVKMEDPEQYSQVAAVLKGQPGIDEIVDQSAFLDRLF
                     AVTRVFRVGVLLVAFVMLVSASILIANTVRMGLFARRKEIGIMKLVGATNWRIRLPFL
                     VEGLFESLIGAIAAILFLFAIKVVFIEPLNESVQFIPWIVNKDLIAIIPILLVAGAIV
                     ALLAGLVGMRRFLDV"
     gene            6221..6697
                     /gene="smpB"
                     /locus_tag="VIH70_11035"
     CDS             6221..6697
                     /gene="smpB"
                     /locus_tag="VIH70_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018729677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY8116938.1"
                     /translation="MSPKGDPEGQKTVVSNRRARHDYEILERYETGIVLTGAEVKSLR
                     GGRGSLAEAFARVRGDEVWLEGMHIPPYEQAMDKARWDPIRARKLLLHRKEIERLIGK
                     TAEKGLALIPLRVYFAHGLAKVELGLGRGRRQFEKRQAIAEREHRREIEREVGRRR"
     gene            6742..>6928
                     /gene="ssrA"
                     /locus_tag="VIH70_11040"
     tmRNA           6742..>6928
                     /gene="ssrA"
                     /locus_tag="VIH70_11040"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1181 a         2379 c         2447 g          921 t
ORIGIN      
        1 gaacctccgg ggcgaacgca aggggatcga cttcggctcg cagatccgct cctacgtcct
       61 ggcgccgtac cggctcgtca aggaccaccg gaccaacgtc gagatcggcg acgtcgatcg
      121 ggtcctggac ggcgatctcg aggagctgat cgaggccgag ctccgccgaa gggccgaggt
      181 tggggaaaac cccaagcgga acggccggct cctcggacca tcgcaatagg tccttggccg
      241 tcgccgtccc gagtgaggcc gagatgcgcc cggcatgctt gtcgttgggt ggcggagccg
      301 agagaatcgg ttcgtctgcg ggaggagcct gagcgctgtc cgagaagacc aaccgtggcc
      361 tcgaggtctc gatcaccacg gttttcgttc tggcgctgct gtcggtcgcc gcggtcctga
      421 tcttctccgg tgaacccgcg tccgccaaac agttcgcgac gctccgcatc ctcgatggcc
      481 aggtcgcggt ccagcgcggc agcggcgcct tcgagacggg gcaggacggc gcgtcgctcc
      541 acgagggcga tacggttcgg accggccccg atgggcgggc ttcgatcgac tacttcgacg
      601 ggagcctcac ccgcctcgac ttcgacacgt cattcaccct ggtgaccctc gagaccctgc
      661 gcgatacggc ggcctcgatc atcgagagca gtcaggccga aggcaattcc taccaccgcg
      721 tgccggagct caccgacgcg cagagccgct tcgaggtcca gaccccgacc gcgaccgcgt
      781 cggtccacgg cacgggatac gcgttgctgg tggatgaggg atccacgacg atcgccgtgg
      841 tcgagggagt ggtcactgcc gggggatcca ccgggtccgt cgagatcccc gcgggcacga
      901 tggcggtcgt gggaaccgac ggcgtcgttg ggccgatccg ggcgatccct tcggagctgc
      961 tcgacagcga ctggctcaag ttcaaccgat gccccgatca cgacgccccg gcgtgtgccg
     1021 agggcgacgg aggcgggccg gaaggcgacg gaggcgggcc cgagggaccg accgaaccgc
     1081 cggaggggtc cggccagggg gagcaggggt cggtcccgcc cacatccacc gggggcgccg
     1141 gcggtgaggg caccacgggc gccggcgggc agagcggcac gtccggcggt ggggccggtg
     1201 gtacgacgcc accgcgcaac cagcccccgc aggcggggtt ctcggcaacc ccacggctcg
     1261 gaccggctcc cctgcgtgtc gggttctcgt atgcgtcgca cgaccccgac ggcgatccgc
     1321 tgtcgcggca ttggagcttc ggtgacgggt cggcacagtc gggcggccag actccttcgc
     1381 acacgtacga cgatcccggc cggtacaccg tgacgctcac cgtgtcggac ccgaagggca
     1441 agcgggacgc gacgagcaag gtcatccggg tcgcatcggc ggaggcgacc ctcgatcaca
     1501 tcatcatctc cccctcgaac gcgacgatcc ggccaggaga ctcccgctcg tacacggcgg
     1561 aggcgttcga taccgatgga ggctcgatgg gcgacgtcac ggtcgacacg gacttctcga
     1621 tcgcgcccaa ggggtcgtgc aaggacaaca cgtgcacggc aaaacggccc ggagcgcaca
     1681 cggtgacggg gacctaccac ggcgaaagcg accaggcgaa gctcacggtc gaggaggcac
     1741 caccgccacc accgtcgtgc cccgcctatg cgctggcatt ccacgctcgt cccccggcga
     1801 accagtcggc ggggcaccag ttcaacgcgc agatccgcgt cgacgtcctc ggcggatcga
     1861 gcgacggacc gctgagcatc gacctgtccc tgcagggcgg cacgttcagt ggcggcgaga
     1921 cctccgcgac gtggaccggg cagggggtcg tgaacttcaa tcacctcacg atcgacgggc
     1981 cggggagcta cgcgatctcg gcctcggcaa gctgcgcgac ctcgccggac cccgcacccg
     2041 tcacgatcac cgacggctcg aacgggagcg ggaagaaggg gggttcggcg atcggtctgg
     2101 tgctggcgct cccatggatg cgccggctcc gtcgcgcgcc ctgagccgac ccctcacgat
     2161 cacgaacacc agcgcgaccg cgaacccgag ccaggcgatt gccgagagcg ccaggccggt
     2221 cccgatcgcc ggctcccggt acgtgagccg gacgtcgtgg tggcccggcg ggagcgcgat
     2281 cccctggagg aacccgtcgg tgcgcagcac cggcaccgaa cgcccgtcga gcgtcgccga
     2341 ccacccctcg tcccagacgt tgcggacgag caggatcgac gggacggagg cttccgcctc
     2401 gatccgcacg tcctcggggg ccacctcgag atacgcggcg gtgcccccga tgcccgccgc
     2461 gcccggggtc gagcccggat cggtctcgag caccgcgacc gcggccggat cgaaccccgg
     2521 ctccagggcg gcgtcgaggg ccgcccgctc gccgctcacc accgtccacg agggaaccac
     2581 ggatgcccgc ggctcggcgt cgtcgatctc gacgaggcgg taggcgccct cggtctcgat
     2641 cgtccgaccc gacacgcccg gcggcagcgg cctcgggatc ccctgcggca cgatcaggta
     2701 ccgggccccg aggagccgga ggtcggtgag cgtcggcacc tggagcaccg cggcgttgta
     2761 gaagacgggg aacctgttcg tggcgtggac gtagtcccag tagccgggcg gctggacggg
     2821 ggagtagccg atcgtgtccg gaatctcgaa cagcacggat cgtccgatca gcaacgccgg
     2881 ccagtcgggc tccttccggg tgaacaggta gcccttgttg aagtacgcgt cggggcggat
     2941 ccacgcgagg tagcgcccgt cggaggcccc ggagcgttgc agcgctcgag cgatcgggcc
     3001 gggcgagagg tactcgtgca cgtcgacgtc gggccaccgg agcggcgcgg cgaccagcgc
     3061 cgcgtggaga ccggtctcga ggccgaggta caccgtgccg ccccggtaca tcgacgacca
     3121 gatcgcgccg gccaggagct cgacggccag gatcgcgcag agcgcgaccg gggcccaccg
     3181 gcggcgatgc gccagcgccc acacgacgcc gatcaccgcg acggacgcga tcgcgaagat
     3241 cgtgagccgc ccgacccgcg cgcccatcag caacgggaag accaggagga cggcgagtcc
     3301 cgcgccgatc cagcgcatcg cctccccgaa cgccggccgt tgatccatga cccactggac
     3361 cccgatcgcg ccgagggccg ggacgatcag gaacgcgagg tagcgcaggc ggctcgggtt
     3421 gtgcaggtag acgtcgccga acggcagcga cagcgcgaac gaacggaagc accccgcgcc
     3481 caccagcagc gtgatcgtca acaggtacgc gagcgccgct accgcgccca gggcgacgac
     3541 gaggtaccgc cgcgccgagt ctcggaccgc gaacggcacg aacagcagga cggcggcccc
     3601 cacgtacccg cccggcgtcg aagcgagcgc gagcggccag cctccccaga tgccggtcgc
     3661 cgggatcggg acgtcctccg tcgtcgcccg cgcgcgcgca accgtgcctt cgatcgcgcc
     3721 gtacccgtcg gccaggctcg accgcgcgag gaccgcgaag tgcgggacca cgatcgcgag
     3781 gttcgcgagc ggaaggaagg ccaggaacgc caccgaccac ccgaccgcgg cacccgggtg
     3841 gagcgtgccc cgacgcgtct cgcgtatcgc gcgcgcgacc acgtacgcga cgaccaggcc
     3901 cgtggccatc accagcccgt gcgagaggtg ggaggtcgcg acctgacccc acgcgaacgc
     3961 cgcgagcgcg agccacgcga gccgccgcca acccgcgcgg gcgaagaacc cggacgcgcc
     4021 gaccaggatg aacggcgtcc atgcgagcgt gccggcgaac ggaagactga ccgctaggat
     4081 cgacgcggag accgccatcg agagcgagag cccgccggcg gtggccgcga tccggccgag
     4141 cccctccttc cgcaggaacc agaacagccc gaggccggcc aggatcgggt tcaggacgat
     4201 cagcgcacgg agcccaccgc cgcagccgaa cacccacgac gaggccatca tcggcaacga
     4261 gagccacccg ctctgcgggt ctgccgcgaa ccaggtccct gacatctcga acgggttcca
     4321 tagcggcacg tggccgtcgg cgatcgcgcg cccgagcatg cacgaacggg ggagccagaa
     4381 ggacaggacg tcgggatgct ggtccgacag gttcggcagg aacgcgatgc cccgcaacgc
     4441 gatcaggacc aaccccacga tgagggtggg accggcgacc gcgttcagga cacggcgagc
     4501 cgtcgagggc gcgggaggct cgtcggcgcg cctggggtcg cgcggcgggc gcgtcgccgt
     4561 ctccatcggc tcaggctatc ggggccgctt cgttcctgtt ggccaggggg tctgttagac
     4621 tccgcgccgg ttcggccccc cggatcaatc taggacccca tgatcagtct tcagcacgtc
     4681 acgaaggtgt accggaacgg gacgacggcg ctcgaggacg tcaccgtcga ggtggagaag
     4741 ggtgagttcg tcttcatcgt cggccaatcg ggctccggca agagcacgtt catccgactc
     4801 ctgctgaagg aggaggaggc gtccaagggc gacatctacg tcgccggcaa gaacctggcc
     4861 aagctgacca cctggaaggt gccgcacctg cgccggaaca tcgggacggt gttccaagac
     4921 ttcaagctcc tgcaggacaa gaccgtgttc gagaacgtcg cgttcgcgct cgaggtgatc
     4981 ggcaagccca agcacgtgat cgaccagcgt gtgcccgaga tcctggagct cgtcgggctc
     5041 ggcgacaagc tcaacaactt ccccgacgag ctctccggcg gcgagcagca gcgcgtgtcg
     5101 atcgcccgag cctgcgtgaa ccgcccgctg gtgctcctgg ccgacgagcc gaccgggaac
     5161 ctcgacccct cgacgtcggt cgagatcatg cgcctgctcg acaaggtgaa ccgcatcggg
     5221 acgacggtcg tcatggccac gcacgaccag gcgatcgtgg acgcgatgcg ccgccgggtg
     5281 atcgagctcg acaagggcaa ggtcgtccgc gaccaggctc gcggggtgta cagctagtgc
     5341 cgctcgtcga gtatctgttc cgcgagacca cctcgggtct gcgccggaac gggctcgtcg
     5401 cgttcgcggc gatctcgacc acgttcatcg cgctgtttct gttcgggctg tcgctgctga
     5461 tctcgcggca ggtgagcctg atgatcgagg cgacgaccgg caacgtcgag gtggccgtgt
     5521 acctcaccga cccggtgaac ccctcgaccg tgagctcgct cacgagcacg ctcaccgacc
     5581 tccccgtggt ggcgaacgtc gactacgagg acaagcagga ggcgtgcgac cgcttccgga
     5641 ccctgttcgc ggagcagaag gcgctggtga acaacgtcga ctgcgatgcg ctgcccgcct
     5701 cgctccgggt caagatggag gatcccgagc agtactccca ggtcgccgcc gtgctgaagg
     5761 ggcaaccggg catcgacgag atcgtcgacc agagcgcgtt ccttgaccgg ctgttcgccg
     5821 tcacacgggt gttccgggtc ggcgtgctgc tcgtcgcgtt cgtgatgctc gtgtcggcgt
     5881 cgatcctgat cgcgaacacg gtccggatgg ggctgttcgc gcgccgcaag gagatcggga
     5941 tcatgaagct cgtgggcgcg accaactggc ggatccggct cccgttcctc gtcgaggggc
     6001 tgttcgagag cctcatcgga gcgatcgcgg cgatcctgtt cctgttcgcg atcaaggtcg
     6061 tgttcatcga gcccctgaac gagtccgtgc agttcatccc gtggatcgtg aacaaggacc
     6121 tgatcgcgat catcccgatc ctgctcgtcg cgggagcgat cgtggccctg ctcgccggcc
     6181 tcgtcgggat gcgccgcttc ctcgacgtct aaccttcggc atgagtccga agggcgaccc
     6241 ggagggtcag aaaaccgtcg tctccaaccg ccgggcccgg cacgactacg agatcctcga
     6301 acgctacgag acgggcatcg tgctcaccgg ggccgaggtg aagtcgctcc gcggcggacg
     6361 cggttcgctc gccgaggcgt tcgcgcgcgt cagaggcgac gaggtgtggc tggaggggat
     6421 gcacatcccg ccgtacgagc aggcgatgga caaggcccgc tgggacccga tccgggcgcg
     6481 gaagctcctg ctgcatcgca aggagatcga gcgcctgatc ggcaagacgg ccgagaaggg
     6541 gctcgcgctg ataccgctgc gggtctactt cgcgcacggg ctggccaagg tggagctcgg
     6601 gctcgggcgg ggcaggaggc agttcgagaa gcgccaggcg atcgctgagc gggagcaccg
     6661 gcgcgagatc gagcgcgagg tcgggcggcg gcgctagccg ggccggggcg ggcccgtata
     6721 ctcgttggta caaccgaata tgggggtgca ctggcttcga cctgagcgag aaccgctcag
     6781 gggaagcggg ccgaggtccc cggatgcctc gttaaaagcc gggaccaaca caagcgccga
     6841 taacgattac gcgctcgctg cctaggtagc taggtagccg tcccctcccg gtcgcctgcg
     6901 acgggaggat gggcgtcgtg agcaggct
//
LOCUS       DATBRK010000373         4215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1806844, whole genome shotgun sequence.
ACCESSION   DATBRK010000373 DATBRK010000000
VERSION     DATBRK010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806844"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1027
                     /locus_tag="VIH70_11045"
     CDS             236..1027
                     /locus_tag="VIH70_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8116939.1"
                     /translation="MDDWRSYDSVAETYARIHAPRLAEPARDLVELTGVDAGWRVLDV
                     GTGTGVAAFAAAGRGANVVGIDEAAGMLKVARSLNGSLPVANALAIDLPFPDGAFDAL
                     LANFVLAHFTKYKTALFDMKRVLRPGGRLGLTAWADAEDDLARTWLELVGTVVPSEVL
                     QAAMDERLPWRERFRQRLPLEEALMEAGIRHVRTEVRRYRFQYSLDDYVEGLSTWATG
                     RFVRFMLGEAAFAEFLGRARVAFGERFADPVNDFREVLFATGTKP"
     gene            complement(1076..2584)
                     /locus_tag="VIH70_11050"
     CDS             complement(1076..2584)
                     /locus_tag="VIH70_11050"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY8116940.1"
                     /translation="MPTFAFSTDRATELAVDVLVLPVFEGPQAGPGIKDVKGLDLLGQ
                     FSATGAKGKRGESLLVPTGGGRTPAAKAVLLVGVGPKNAATPDACRRAIGLVARQLAR
                     RRTVATTLPQIVGAKDKLEAIQATVEGVLLGSYRFDRYKSKSDDADRRLGRVTVLGAG
                     KADAKAAKEAREAIRRGEVIAESQMWARDLVNTPALDLPPAEIAREAQAMAKQVGLTS
                     KVWTEAELKKGGFGGVLGVGQGSVRPPRLIELKYSGGGKGSPIALTGKGIAFDSGGLS
                     IKDATGMEWMKADMGGAASILATMRAIALLKPKINVIAAIPSAENMPSGSAIRPGDVL
                     THRGGTTSEVLNTDAEGRLVLADSLAFLAEKKPTVIIDTATLTGACMIALGTDIWGAM
                     GNDRDLIRDVLAAGEAAGEPGWELPMHEPYRKQIDSDIADIKNIGKRFGGAITAALFL
                     REFVGEVPWVHLDIAGPAFAERPGDYWPKGATGSPVRALVRYVLTKADGARS"
     gene            2700..3038
                     /locus_tag="VIH70_11055"
     CDS             2700..3038
                     /locus_tag="VIH70_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116941.1"
                     /translation="MDARDLRDLVRFDEDGPHHEELFESEHLWSEVVCLERNQSLGTI
                     EDRDSDALVLVVTGRVVVQVNLGRKRLEQWETTLVPAGSELTITNATGEPAVVLLVAA
                     PPPPKRPISE"
     gene            complement(3028..>4215)
                     /locus_tag="VIH70_11060"
     CDS             complement(3028..>4215)
                     /locus_tag="VIH70_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY8116942.1"
                     /translation="TGRAFLAIGVGTLIGCVPLALVGLAGEREGVPTMVLLRPVLGAR
                     GSFMPTGLNVVQLVGWTAVEFWAMGGVANVASARLFGFESRVFWLAVVAVVCTALAIG
                     GPVLVVRRWLERFGIYVLLGSAVWITVEILRGSGAFWGSAGGGGLPFWLAVDLVIVMP
                     VSWLPLAADYNRFARLGSRATLGTYAGYAAGNAWFYALGVLLVLATGANADALGIGSA
                     IVGLAGGAVVLVALLVGESDNAFADIYSAAVSTQNVASKAPQRGLVLAVGALGFLLAL
                     AFTADRYELFLFLIGSVFVPLTGVFLADFFVRNHGRYGQAAVFEPTGVRWLAFVPWVV
                     GFVVYQWCVPTGPQGWVDAVERVFDAVGLPFPLFDSGLGASLPSFVVAFALTLLLPRR
                     ATR"
BASE COUNT          647 a         1554 c         1471 g          543 t
ORIGIN      
        1 agcagcacgc cgccggtgag ggtgaagaag ccgcgcctga cctcggtcca cagcggcgtg
       61 aggaacagca gcacgagccc gcccgagacg gtctcggcca ggacgagcgc ggtgacgccc
      121 gcgggcccgg acacgccgag agggtacccg cgggacgacg gcctcccgtt gcgacggaac
      181 ggtcggggac gacgtggtcc tccgaaccgc ggggacgccg gtaggctgga cggccatgga
      241 cgactggcgg tcctacgaca gcgtcgcgga gacctacgcg cggatccacg cgccgcgcct
      301 ggccgagccc gcgcgcgacc tcgtcgagct cacgggggtc gatgccgggt ggcgcgtgct
      361 cgacgtcgga acggggaccg gcgtggcggc gttcgcggcc gccggcaggg gcgcgaacgt
      421 cgtggggatc gacgaggcgg ccgggatgct gaaggtcgcg aggtcgctga acggctcgct
      481 gccggtcgcg aacgcgctcg cgatcgacct ccccttcccc gacggcgcgt tcgacgccct
      541 gctcgcgaac ttcgtgctgg cgcacttcac caagtacaag acggcgctgt tcgacatgaa
      601 gcgggtcctc cgcccggggg ggcgcctggg acttacggcc tgggccgacg ccgaggacga
      661 cctggccagg acgtggctcg agctcgtggg gacggtcgtg ccgtccgagg tcctgcaggc
      721 cgcgatggac gagcggctcc cgtggcgcga gcgcttccgc caacgcctgc cgctcgagga
      781 ggcactgatg gaggcaggga tccgccacgt ccgcaccgag gtgcgccgct accggttcca
      841 gtactccctc gatgactacg tcgaggggct ctccacgtgg gcaaccggcc gattcgtccg
      901 cttcatgctg ggcgaggccg cgttcgcgga gttcctcgga cgcgcgcgcg tggcgttcgg
      961 agaacgcttc gccgacccgg tgaacgactt ccgcgaggtc ctgttcgcga ccgggaccaa
     1021 gccctagacc aagacctcgg gaggaccggc gccgagcacc ccgagcgccg tcgcgtcacg
     1081 acctcgcgcc gtccgccttc gtcagcacgt accggacgag cgcgcggacg gggcttccgg
     1141 tcgcgccctt gggccagtag tcgccggggc gttcggcgaa cgcgggaccg gcgatgtcca
     1201 aatgcaccca cggcacctcg ccgacgaact cgcgcaggaa caacgccgcc gtgatcgccc
     1261 cgccgaagcg cttgccgatg ttcttgatgt ccgcgatgtc cgagtcgatc tgcttgcggt
     1321 agggctcgtg catcgggagc tcccacccgg gctcccccgc ggcctcgccc gcggcgagca
     1381 cgtcgcggat caggtcgcgg tcgttgccca tcgcgcccca gatgtcggtc ccgagggcga
     1441 tcatgcacgc gccggtgagc gtcgccgtgt cgatgatgac cgttggcttc ttctcggcca
     1501 ggaaggcgag cgagtccgcc agcaccagcc ggccctcggc gtcggtgttc aggacctcgg
     1561 acgtggtgcc gccgcggtgc gtgagcacgt cgccgggccg gatcgcggaa ccgctcggca
     1621 tgttctcggc cgaggggatc gcggcgatca cgttgatctt cggcttgagc aacgcgatcg
     1681 ctcgcatcgt cgccaggatc gacgccgcgc cgcccatgtc ggccttcatc cactccatcc
     1741 cggtcgcgtc cttgatcgag agccctcccg agtcgaacgc gatgcccttg ccggtcagcg
     1801 cgatcggcga gcccttcccg cccccggagt acttgagctc gatcagccgc ggaggccgga
     1861 cgctgccctg acccacgccg agcacgccgc cgaacccgcc cttcttcagc tctgcctcgg
     1921 tccagacctt gctggtgagc ccgacctgct tggccatcgc ctgggcctcc ctcgcgatct
     1981 ccgccggcgg gagatcgagc gcgggcgtgt tcaccaggtc gcgcgcccac atctgtgact
     2041 cggcgatcac ctcgcctcgc cggatcgcct ccctcgcctc cttggccgcc ttcgcgtcgg
     2101 ccttgccggc gccgaggacc gtgaccctgc ccaggcgtcg atccgcgtcg tccgacttgg
     2161 acttgtagcg gtcgaaccgg taggagccga ggagcacgcc ctcgacggtc gcctggatcg
     2221 cctcgagctt gtccttcgcg ccgacgatct gcggcagcgt cgtcgcgacc gtcctgcgac
     2281 gggcgagctg tctcgcgacc aggccgatcg cccgccggca cgcgtcgggg gtcgccgcgt
     2341 tcttcggccc gacgccgacg agcaggacgg ccttcgcggc cggtgtccgg cccccgccgg
     2401 tcggcaccag gagggactcg ccgcgcttgc ccttggcccc cgtcgcggag aactgcccga
     2461 gcaggtcgag ccccttcacg tccttgatgc ccggcccggc ctggggccct tcgaagaccg
     2521 gcaggacgag cacatcgacg gcgagctccg tggctcggtc ggtcgagaag gcgaacgtgg
     2581 gcacgatggg ctccttgacg ggtcggggac gacagcgcgg aagtctaccg atggcggatg
     2641 gcgatcgcgg atggcccgcg gccgacggca cgtgggtcgg ctcgataggc tgggccgccg
     2701 tggacgccag agaccttcgc gacctcgtcc gcttcgacga ggacgggccg catcacgagg
     2761 agctgttcga gtccgagcac ctgtggtccg aggtcgtctg tctcgagcgg aaccagtcgc
     2821 tggggacgat cgaggaccgg gactcggatg cgctcgtgct cgtcgtgacc ggccgggtcg
     2881 tcgtgcaggt gaacctgggc cgcaagcgcc tcgagcagtg ggagacgacg ctggtcccgg
     2941 cgggctcgga gctgacgatc acgaacgcga ccggcgaacc cgccgtggtc ctgctcgtcg
     3001 ccgcgccccc gccgccgaag cgcccgatca gcgagtagcc cgccggggca gcagcagcgt
     3061 cagggcgaag gcgaccacga agctcggcag gctcgccccc agccccgaat cgaacagcgg
     3121 gaacggcaac cccacggcat cgaacacccg ctcgaccgcg tcgacccagc cctgcggccc
     3181 ggtgggcacg caccattggt agacgacgaa cccgacgacc cacggcacga aggcgagcca
     3241 ccgcacgccg gtcggctcga acaccgccgc ctgcccgtac cgcccgtggt tgcgcacgaa
     3301 gaagtcggcg aggaagacgc cggtgagcgg cacgaagacc gacccgatca ggaacaggaa
     3361 cagctcgtag cgatcggcgg tgaatgcgag cgccagcaga aacccgagcg cacccaccgc
     3421 gagcaccagc ccgcgctgag gcgccttgct cgcgacgttc tgcgtcgaga ccgcggccga
     3481 gtagatgtcg gcgaacgcgt tgtcgctctc cccgaccagc agcgccacca gcaccacggc
     3541 gccgccggcc aggcccacga tcgccgagcc gatccccagc gcgtccgcgt tcgcgccggt
     3601 cgcgagcacc aacagcacgc cgagcgcgta gaaccaggcg ttgcccgcgg cgtagccggc
     3661 gtacgtgccc agtgtcgcgc gcgagccgag tcgcgcgaag cggttgtagt ccgcggcgag
     3721 cgggagccag gagaccggca tcacgatcac caggtccacg gcgagccaga acgggagccc
     3781 gccccctccg gccgagcccc agaacgcgcc cgagccccgc aggatttcga cggtgatcca
     3841 caccgccgag ccgagcagca cgtagatccc gaagcgctcg agccaccgac gcacgaccag
     3901 cacggggccg ccgatcgcga gcgcggtgca cacgacggcc acgacggcga gccagaacac
     3961 ccgcgactcg aacccgaaca gccgcgccga ggcgacattc gcgaccccgc ccatcgccca
     4021 gaactcgacg gcggtccacc cgacgagctg cacgacgttc agccccgtgg gcatgaacga
     4081 gcctcgcgcg cccaataccg ggcggagcag gaccatcgtg gggacgccct cgcgctcgcc
     4141 ggcgagcccg accagggcga gcgggacgca cccgatcagc gtgcccaccc cgatcgcgag
     4201 gaatgcgcgc ccggt
//
LOCUS       DATBRK010000374         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_934654, whole genome shotgun sequence.
ACCESSION   DATBRK010000374 DATBRK010000000
VERSION     DATBRK010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934654"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /gene="glmS"
                     /locus_tag="VIH70_11065"
     CDS             <1..1506
                     /gene="glmS"
                     /locus_tag="VIH70_11065"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY8116943.1"
                     /translation="RARLEKDGHTLTSETDTELVAHLIEGRLRDGASLVEAIRGTVRE
                     LEGAYSLVVLSSAEPDVLVGVKVSSPLVVGLADGETILASDIPAVLERTRTVIPVDEG
                     RIVEVRAGGVRFTDFEGNVVTPEPITVDWDAGQAEKSGFDTFMLKEIHEQPAAIRDTL
                     VGRVGEQGLLALDELHTDTDVLREIDKVFVVACGTAFHSGLVAKYAIEHWTRVPVEID
                     IASEFRYRDPVLGPNTLTLAVSQSGETIDTLEAARHASRQGSKVIAVTNTIGSSLARE
                     ADAVMYTHAGPEICVAATKTFATQMAALHLVALYLAQIRGAMFPVEVGDILTRMRELP
                     GQVRRTLSLSKQILGIAHEIHEARDVLFIGRHTGYPAALEGALKLKELSYVHAEGYPA
                     GELKHGPIALVEEGVPVVAVATECHVYPKVLSNIQEVRARGARVIAIASEGDEEISRH
                     ADDVLFVPRVPELFAPVVVTPPLQLLAYHVAKLRGCDVDQPRNLAKSVTVE"
     gene            1543..1917
                     /locus_tag="VIH70_11070"
     CDS             1543..1917
                     /locus_tag="VIH70_11070"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY8116944.1"
                     /translation="MEILGLGVDICEIERMERALGRHAAMRERVFTPEERAYCDARAR
                     PAESYAGRFAAREAVIKALGGYRGRRWQDISVTRAPSGAPSVRLEGNAKARADRLGIS
                     DVLITFTHERSSAVAFAVAVSG"
BASE COUNT          300 a          703 c          791 g          294 t
ORIGIN      
        1 cgggcccggc tcgagaagga cggtcacacg ctcacgtccg aaaccgacac ggagctggtg
       61 gcgcacctga tcgagggtcg cctccgcgac ggcgcgtcgc tcgtcgaggc gatccggggg
      121 accgtccgcg agctggaggg ggcctactcg ctcgtcgtcc tttcctccgc ggagccggac
      181 gtgctcgtcg gcgtgaaggt ctcatcgccg ctggtcgttg ggctcgccga cggggagacg
      241 atcctcgcct ccgacatccc ggccgtcctg gagcgcacca ggaccgtgat cccggtggac
      301 gagggccgga tcgtggaggt gcgtgccggg ggcgtgaggt tcacggactt cgagggcaac
      361 gtcgtcacgc ccgagccgat cacggtcgac tgggacgccg ggcaggcgga gaagagcggc
      421 ttcgacacct tcatgctgaa ggagatccac gagcagcccg cggcgatccg ggacacgttg
      481 gtcggccgcg tcggcgagca gggtctgctc gcgctggacg agctgcacac cgacaccgac
      541 gtcctgcgcg agatcgacaa ggtgttcgtc gtggcgtgcg ggacggcgtt ccactccggg
      601 ctggtcgcca agtacgcgat cgagcactgg acgcgggtgc cggtcgagat cgacatcgcc
      661 tcggagttcc gctaccgcga tcccgtgctc gggccgaaca cgctcacgct cgccgtgagc
      721 cagtctgggg agacgatcga cacgctcgag gcggcgcggc acgcgtcgcg ccagggctcg
      781 aaggtgatcg ccgtgaccaa cacgatcggc tcgtcgctcg cgcgggaggc cgacgcggtg
      841 atgtacacgc acgcgggacc cgagatctgc gtggcggcga cgaagacctt cgccacgcag
      901 atggccgcgc tgcacctggt ggcgctgtac ctcgcccaga tccgcggcgc gatgttcccg
      961 gtcgaggtcg gcgacatcct gacccggatg cgcgagctcc cgggccaggt tcgacgcacg
     1021 ctttcgctgt cgaagcagat cctcggcatc gcccacgaga tccacgaggc ccgcgatgtc
     1081 ctgttcatcg gacggcacac cggctacccc gccgcgctcg agggggcgct gaagctgaag
     1141 gagctgtcgt acgtgcacgc ggaggggtac cccgccggcg agctcaagca cgggccgatc
     1201 gcgctcgtcg aggagggcgt cccggtcgtc gcggtcgcaa ccgagtgcca cgtctatccg
     1261 aaggtcctgt cgaacatcca ggaggttcgc gcgcgcggcg cccgcgtgat cgcgatcgcg
     1321 agcgagggcg acgaggagat ctcgcgccac gccgacgacg tgctgttcgt cccgcgggtg
     1381 cccgagctgt tcgccccggt ggtggtgacg ccgccgttgc agctcctggc ctaccacgtc
     1441 gccaagctcc gggggtgcga cgtcgaccag cctcggaacc tcgccaagag cgtcacggtc
     1501 gagtagccga gcggggggcc cctcggcggc tacgctagcg gcgtggagat cctcggcctc
     1561 ggggtcgaca tctgcgagat cgagcggatg gagcgcgccc tggggcggca cgcggcgatg
     1621 cgcgagcggg tgttcacccc ggaggagcgg gcctactgcg acgcgagggc tcgtccggcg
     1681 gagtcctacg cgggacggtt cgcggcccgg gaggccgtga tcaaggcgct cggcggctac
     1741 cggggcaggc gctggcagga catctccgtc acccgggcgc cttccggcgc gccctcggtc
     1801 cgcctggagg gcaacgcgaa ggccagggcg gaccgtctcg ggatctcgga cgtgctgatc
     1861 acgttcaccc acgagaggtc gagcgcggtg gcgttcgcgg tggcggtgtc cggatgaggt
     1921 tcgtcctgac ccccgcggag gcggccgagc tcgatcgcgc gacccaacgg cgagggatcc
     1981 ccgcggacgc gctgatggag gcggccggcc gcgaggtcgc cgacgccgcc gtccggctga
     2041 tcggcggcgc gtacgggcgg cgcgcggtgg tggtgtgcgg caagggca
//
LOCUS       DATBRK010000375         8267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_74310, whole genome shotgun sequence.
ACCESSION   DATBRK010000375 DATBRK010000000
VERSION     DATBRK010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74310"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VIH70_11075"
     CDS             <1..325
                     /locus_tag="VIH70_11075"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HEY8116945.1"
                     /translation="TGRTLAEVFDAFAPEDEQGFSTSRVPIRLFEIGTGFVSRSEARR
                     VAHRLERFRRVELDFSGVDELGQGFADELFRVWPSRHPEVRLEPTNMSAVVARVVDRA
                     RAERA"
     gene            395..1636
                     /gene="ccrA"
                     /locus_tag="VIH70_11080"
     CDS             395..1636
                     /gene="ccrA"
                     /locus_tag="VIH70_11080"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEY8116946.1"
                     /translation="MAKDVYDVGDIPPVGEVPPRMHAQLVRPERYGEPEDAIRDEVID
                     VPSIGPDEALVLVMAAGVNFNNVWAARGVPVDVTTTQARWGEPTDFHIVGSDAAGVVY
                     AVGERVTNVRVGDRVVVHAGQWDLEDPWVVEGGDPGLAPSFRVWGYDTCWGSFAQFTK
                     VQAHQCLPKAESLTWEEAASPTLTGATAYRMLHGWPPNTVKRGDVVLVWGASGGLGSL
                     AVQLVALAGARAVAVVSSADRGAFAVDQGAVGFIDRTAFDHWGTPPAWNAPEWRTWFE
                     GAKAFGKAIWEVVGERASPNLVFEHPGQDTIPTSIFVCERGGMVVICAGTTGYDTMVD
                     VRYLWYLQKRYQGSHLFNDEQATAFNDLVREGRVRTTLGHTYDYGESGHAHQLMEQGA
                     LPEGNVAVLINAPERGRTDLP"
     gene            complement(1633..2061)
                     /gene="mce"
                     /locus_tag="VIH70_11085"
     CDS             complement(1633..2061)
                     /gene="mce"
                     /locus_tag="VIH70_11085"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEY8116947.1"
                     /translation="MLTQIDHVGIAVADLEEEVERYRRTFGLEPIHRERMRDQGVEEV
                     LFAVGESYVQLLGALGSDTQVGRFLGRRGPGLHHLGYRVANVTLALERLRAEGVGLID
                     ETPRAGSRGTTIAFVHPRSLGGVLVELVQEPSPAAAGPRG"
     gene            complement(2061..2456)
                     /locus_tag="VIH70_11090"
     CDS             complement(2061..2456)
                     /locus_tag="VIH70_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116948.1"
                     /translation="MARKKLQPVTKEDARAIGAMLRGLRRAAGYRAVQDAAAAGGFPA
                     ARQTIYAYERGGLTPSLQQFLEITEFYALHPSRGDGTKPEDDLRAQAVASVTRALTLP
                     AYHVKQAHELMERLQPRLAEGPRRKRRSS"
     gene            2623..4449
                     /locus_tag="VIH70_11095"
     CDS             2623..4449
                     /locus_tag="VIH70_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8116949.1"
                     /translation="MKQTSRSTASVLHGSDAAAQGRADVNKVRERTRRRRLTRLIVGL
                     ALFDIYLWYRYLTDNPFKMPTLGPEAIIFAPIVLIMIMIVAMMAMPLFSGRSPHQIVR
                     PEEIEVGLDEVRGLDNQVDEVGRTLDVFLGYATFREQLGGNPRRGMLFEGPPGTGKTY
                     LAKAMAKQAGVPFLFSAAPAFQSMWFGMTNAKIRSFFRRLRKLARKEGGAIGFIEEID
                     AIGGERGGMNASPDGTGLGRVASSFMGPGGSGMVNELLIQMQSFDQPPFGPRVRARMI
                     EWVNGFRKPERRIPAAKPMYSNILLIAATNRADNLDPALLRPGRFDRRLYFDLPSKSG
                     RRELIDFFLDRKTHHQQLDDDHTREDLAHETFGYTPVMIEHLLDEALLMALRGGRTAM
                     NVQDIHEAKLTEELGFKQPVTYTETDRVSVATHEAGHATVAYFLGKGRRLEVLSIIKR
                     RMALGLLAQGDEEERFTRTRTEIESGVAIALGGLVAEEVFFGESGTGPASDLQHATTL
                     AAQMVGSFGMAGSLVSFDAIAEGPIGSKNLVGKVLADAEGKARVEEILEEQKGRVAKL
                     LEENRDVVIGLRDSLIARDELVGDEIMQQIELVLDSRNPSAR"
     gene            4598..5098
                     /locus_tag="VIH70_11100"
     CDS             4598..5098
                     /locus_tag="VIH70_11100"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8116950.1"
                     /translation="MTSPNDVLRSMFEHHLWATETLIDHLDRLPAEQLDASVPGTYGS
                     MIDTLTHMIDGDTRYLRRLRDPTPPPAEDRVGIALAQLRSEMQEHRRRWDEALADLEA
                     GTLHASILGREDYPDTDPAESMLLIQAIHHGNDHRTQICSILGALELPLPEIDGWDFW
                     ADRHRT"
     gene            5105..5611
                     /locus_tag="VIH70_11105"
     CDS             5105..5611
                     /locus_tag="VIH70_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8116951.1"
                     /translation="MDPGDELVDVVDEQNEVVGVAPRRRVRSENLWHRCTYVFVLNAA
                     AHLYVDRRTDTKDVYPGFYDVGAGGVNAGGESYEACARRELEEELGITAEPTLRFRHR
                     YEGPSGRVWGGAFDVLWDGPIVWQPSEVVWGAFVPFDEADAMIAREPFCPDGLEVFQR
                     CRREVRPS"
     gene            complement(5637..6440)
                     /locus_tag="VIH70_11110"
     CDS             complement(5637..6440)
                     /locus_tag="VIH70_11110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8116952.1"
                     /translation="MQAETAPTSIGGTYSTAALDDVYDKLVKAGMSRDVATRRVYRPF
                     VVMGPAAWTDSWHAPRFAGGFHLHEGQDVFCRDGAPVLAATAGRVEFDIDTLGGRIAR
                     LFLPDGGYWYYAHLSDWNSKVDSGSPVDVGDVIGYCGTSGDAVGGPPHLHFGHYLPSG
                     QAEDPMFDLIGWLEAAERRAGVSERAAEAARSTTVIVPTYPRELGAQPTVIVSSPVVT
                     AGLSTARRSAAPSSSDVLALAVALAARLALVPGRRAARRVGSLIGRGTD"
     gene            complement(7211..8077)
                     /locus_tag="VIH70_11115"
     CDS             complement(7211..8077)
                     /locus_tag="VIH70_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8116953.1"
                     /translation="MPRFGVSIIPSASGRSDPVAQARRAEEIGFDVVSIWDHPHGENP
                     SLETWTLMTWIAARTSRIHVASNVLGLPFRLPALTAKMAETLDRLSDGRLILGLGGGG
                     NDEEFAGFGAPVRTPSEKVEALEEGVAMIRGVWAESPFTYHGRHYRSEAAMLEPKPSH
                     RIPIWLGTYGRKALELTGRVADGWIPSMGPAPPSQVKPKMELVRRAAEHAGRDPDSLE
                     YAYNVSVRIGGPPADDPERRVTGEPDEIVERGLELLDLGFTVLNFWVGGRRDEQQELL
                     ATEVVTPLRQLG"
     gene            8167..>8267
                     /locus_tag="VIH70_11120"
     CDS             8167..>8267
                     /locus_tag="VIH70_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116954.1"
                     /translation="MAPSVIVSGARTPIGKFRGAFAGMSAVDLGAIA"
BASE COUNT         1214 a         2790 c         3008 g         1255 t
ORIGIN      
        1 aaccgggagg acgctcgccg aggtcttcga tgcgttcgcg cccgaagacg aacaggggtt
       61 ctccacctcg cgggttccga tcagactctt cgagatcggg accggcttcg tctctcgatc
      121 ggaggctcgt cgagtcgccc atcggctgga gaggtttcgc cgggtcgaac tcgacttcag
      181 cggcgtcgac gagttggggc aggggttcgc cgacgagctg ttccgcgtgt ggccgtcgcg
      241 ccatcccgag gtccgactcg aacccacgaa catgtcggcg gtggttgcac gagtcgtcga
      301 ccgcgcacgc gccgagcgcg cctgatacct ccttgtcgcg aacgccgacg ctcgcctatc
      361 gtcggccgca cccacgatct cgaacggagc gagcatggcc aaggacgtct acgacgtcgg
      421 cgacatcccg ccggtgggcg aggttccgcc ccggatgcac gcgcagctgg tccggcccga
      481 acggtacggc gaacccgagg acgcgatccg cgacgaggtg atcgacgtcc cctcgatcgg
      541 tcccgacgag gcgctcgtgc tggtcatggc ggcgggtgtc aacttcaaca acgtctgggc
      601 ggctcgcggc gtgcccgtcg acgtgaccac gacgcaggcg cgttggggcg aaccgaccga
      661 tttccacatc gtcggctcgg acgcggcggg agtcgtgtac gcggtcggcg agcgcgtgac
      721 caacgtccgc gtgggcgacc gggtcgtcgt ccacgcgggc cagtgggacc tcgaggaccc
      781 gtgggtagtc gaaggcgggg atcccggtct cgcgccgtcg ttccgcgtgt gggggtacga
      841 cacctgttgg ggctcgttcg cgcagttcac gaaggtgcag gcccatcagt gcctgcccaa
      901 ggccgagtcg ctcacctggg aggaggcggc ctcgccgacg ctcaccggcg cgacggcgta
      961 tcggatgctc cacggctggc cgccgaacac cgtcaagcgg ggcgacgtcg tgctcgtctg
     1021 gggagcctcg ggcggcctcg ggtcgctggc ggtccagctc gtggcgctcg cgggcgccag
     1081 ggcggtcgcc gtcgtgtcgt cggccgacag gggcgcgttc gccgtggatc agggcgcggt
     1141 cggtttcatc gaccggaccg ccttcgacca ctgggggacg ccgcccgcct ggaacgcgcc
     1201 cgagtggagg acatggttcg agggcgcgaa ggcgttcggg aaggcgatct gggaggtcgt
     1261 cggcgaacgg gcgagcccca acctcgtgtt cgagcacccg gggcaggaca cgatcccgac
     1321 ctcgatcttc gtgtgcgagc ggggcggcat ggtcgtgatc tgcgcgggga cgaccggcta
     1381 cgacacgatg gtggacgtcc ggtacctgtg gtacctgcag aagcgatacc agggctcgca
     1441 cctgttcaac gacgagcagg cgacggcgtt caacgacctc gtgcgcgagg ggcgcgtgcg
     1501 gacgacgctc ggacacacgt acgactacgg cgagtccggg cacgcccacc agctgatgga
     1561 gcagggcgcc ctccccgagg gcaacgtcgc ggtgctgatc aacgcgccgg agcgcggccg
     1621 gaccgatctt ccttagcccc gcgggcccgc ggcggccggc gaaggctcct ggacgagctc
     1681 gaccagcacc ccgcccaagc tcctcgggtg gacgaacgcg atcgtcgtgc cccgagaccc
     1741 cgctcgtggc gtctcgtcga tcagcccgac gccttcggcc cgaaggcgct cgagcgccag
     1801 ggtcacgttc gcgacgcggt acccgaggtg atgaaggccc gggccgcgcc ggcccaggaa
     1861 cctccccacc tgcgtgtcgg acccgagcgc cccgagcagc tgcacgtacg actcgcccac
     1921 cgcgaacagg acctcctcga caccctggtc ccgcatccgt tcgcggtgga tgggctcgag
     1981 gccgaacgtg cgccggtagc gctcgacctc ctcctcgaga tccgcgaccg cgatcccgac
     2041 gtggtcgatc tgggtcagca tcacgagctc cggcgcttgc gacgcgggcc ctcggcgagc
     2101 cgcggctgga gccgttccat cagttcgtgc gcctgcttca cgtggtaggc gggcagcgtg
     2161 agcgcccggg tcacggaggc gaccgcctgg gctcgcaggt cgtcctcggg cttggtcccg
     2221 tcgccgcgtg atggatgcaa cgcgtagaac tcggtgatct cgaggaactg ctggagcgaa
     2281 ggtgtcaggc cgccgcgttc gtacgcgtag atcgtctggc gcgcggcggg gaaaccgccg
     2341 gcggccgccg cgtcttggac cgcccggtag ccggcggccc gacggaggcc cctgagcatc
     2401 gcgccgatcg cccgcgcgtc ctccttggtc acgggctgga gcttcttcct cgccatcggc
     2461 acgcacgata gcgggtggcg gatccgcggg cagcgcccat cctcgggcac gtcacgtgga
     2521 gccgcctttc ggcgtccccc tctaggccga aggtccgatg tgccgtcccg acgtcgtgca
     2581 taggatgggg ctcgccgtcg gacgaccagg tgaggggctg acgtgaagca gacgagccga
     2641 tcgaccgcat ccgtactgca cggttcggac gccgcggcgc agggccgggc ggacgtcaac
     2701 aaggttcgag agaggacccg tcgccggcga ttgacgcggc tcatcgtcgg gctcgcgctg
     2761 ttcgacatct acctgtggta ccgctacctc accgacaacc ccttcaagat gcccacgctg
     2821 ggccccgagg cgatcatctt cgctccgatt gtcctgatca tgatcatgat cgtcgcgatg
     2881 atggcgatgc cgctgttcag tggccgatcc ccgcaccaga tcgtgcggcc ggaggagatc
     2941 gaggtcgggc tcgacgaggt ccgcgggctc gacaaccagg tcgacgaggt aggacgtacg
     3001 ctcgacgtgt tcctcgggta cgcgacgttc cgcgaacagc tgggcggcaa tccccgacgg
     3061 gggatgttgt tcgagggccc tccaggtacc ggcaagacgt atctggccaa ggcgatggcc
     3121 aagcaggcgg gcgtcccgtt cctgttcagc gcggcgccgg ccttccagtc gatgtggttc
     3181 gggatgacga acgcgaagat ccgctccttc ttccgtcgcc tgcgcaagct cgcgcgcaag
     3241 gagggcggtg cgatcggctt catcgaggag atcgacgcga tcggcggcga gcgcggcggg
     3301 atgaacgcga gccccgacgg gaccggtctc ggccgtgtgg cgtcgtcgtt catgggtccc
     3361 ggcggctcgg gcatggtgaa cgagctgctg atccagatgc agtcgttcga ccagccgccc
     3421 ttcggcccgc gggtcagggc gcggatgatc gagtgggtga acgggttccg caagcccgag
     3481 cggcggatcc ccgcggccaa gccgatgtac agcaacatcc tgctgatcgc ggcgacgaac
     3541 cgagccgaca acctcgaccc ggcgctgctc cggccggggc gcttcgaccg ccggctctac
     3601 ttcgacctcc cgtcgaagtc cggccgtcgc gagctgatcg acttcttcct cgatcgcaag
     3661 acccatcacc agcagctcga cgacgaccac acgcgcgagg acctggcgca cgagacgttc
     3721 ggctacaccc cggtcatgat cgaacacctg ctggacgagg ccctgctgat ggcgctccgc
     3781 ggcgggcgaa cggcgatgaa cgtgcaggac atccacgagg ccaagctgac ggaggagctc
     3841 ggcttcaagc agcccgtcac ctacaccgag accgatcgcg tctcggtcgc cacccacgag
     3901 gcggggcacg ccacggtcgc ctacttcctc ggcaagggcc ggcggctcga ggtgctctcg
     3961 atcatcaagc ggcggatggc gctcgggctg ctcgcgcagg gggacgagga ggagcggttc
     4021 acccgcaccc gcaccgagat cgagtccggc gtcgcgatcg cgctcggtgg gctggtcgcc
     4081 gaggaggtct tcttcggcga gagcggaacc ggtcccgcct ccgacctcca gcacgcgacg
     4141 acgctcgccg cgcagatggt cgggtccttc gggatggcgg gctcgctcgt gtcgttcgac
     4201 gcgatcgccg agggtccgat cggatccaag aacctcgtgg gcaaggtgct tgccgacgcc
     4261 gagggcaagg cccgggtcga ggagatcctc gaggagcaga agggccgggt cgcaaagctg
     4321 ctcgaggaga accgcgacgt ggtgatcggg ctgcgggact cgctgatcgc gcgtgacgag
     4381 ttggtcggcg acgagatcat gcagcagatc gagctcgtgc tcgattctcg gaacccgtcc
     4441 gcaaggtagg ttggggcgcc gtccgcgctg ctcgccttcg cctacgcgcg gggttcgtgc
     4501 tcgtggaaca ccgttgggct tcggcgtcgc tcgacgacgc gctgaccgca cccctcccac
     4561 ctcgaccacc cggccccgcc gcttgtaggg tcgcgggatg acttctccta acgacgtgct
     4621 gcgttcgatg ttcgagcacc atctgtgggc gaccgagacg ctgatcgacc acctcgaccg
     4681 actccccgcc gaacagctcg atgcctcggt ccccggaacg tatggatcga tgatcgacac
     4741 gctgacccac atgatcgacg gcgacacgcg ctacctccgg cggttgcggg atccaacgcc
     4801 gcctccggcc gaggatcggg tcgggatcgc gctcgcgcag ctccgctccg agatgcagga
     4861 gcatcggcgc cggtgggacg aggcgctggc cgacctggag gcggggacgc tgcatgcctc
     4921 gatcctcggg cgggaggact accccgatac ggatcctgcc gagtcgatgc tgctgatcca
     4981 ggcgatccac cacgggaacg accaccggac gcagatctgc tcgatacttg gtgcgctcga
     5041 gctcccgctg cccgagatcg acggctggga cttctgggcc gaccggcatc gcacctaggg
     5101 tccgatggat ccgggggacg agctcgtcga cgtggtcgac gagcagaacg aggtcgtggg
     5161 cgtggcgccg cgccggaggg tccgttccga gaacctctgg catcgctgca cgtacgtgtt
     5221 cgtgctgaac gcggcggcgc acctctacgt agatcgacgg acggacacca aggacgtcta
     5281 ccccggcttc tacgacgtcg gcgccggcgg agtgaacgcg ggcggtgagt cctatgaggc
     5341 gtgcgcccgc cgcgagctcg aggaggagct gggcatcacc gccgaaccga ccttgcgatt
     5401 ccgccatcgg tacgaggggc cgagcggccg ggtctggggc ggcgcgttcg acgtcctgtg
     5461 ggacggcccg atcgtctggc aaccgagcga ggtcgtgtgg ggggcgttcg tcccgttcga
     5521 cgaggcggac gcgatgatcg cgcgcgagcc gttctgcccg gacgggctcg aggtcttcca
     5581 gcggtgtcgg cgggaggtcc ggccgagctg acgcgggggc gaacggccgg acgtcctcaa
     5641 tcggttccgc gaccgatgag cgacccgacc cggcgggccg ctcgacggcc cgggacgagc
     5701 gccaggcggg cggcgagggc caccgcgagt gcgagcacgt cgctggacga cggagcggcg
     5761 gatcggcggg cggtcgagag cccggccgtc acgaccgggc tcgagacgat caccgtcggt
     5821 tgcgctccga gctcgcgggg ataggtcgga acgatcacgg tcgtcgacct cgccgcctcg
     5881 gcggctcgct cggagacgcc ggcacggcgc tccgccgcct cgagccaccc gatgaggtcg
     5941 aacatcggat cctcggcctg ccccgacggt aggtagtgtc cgaaatgcag gtgcggcggc
     6001 ccgccgaccg cgtctcccga ggtcccgcag tatccgatca cgtcgccgac gtcgaccggg
     6061 gagccgctgt cgaccttgga gttccagtcg gacaggtgcg cgtagtacca gtagcccccg
     6121 tcgggcagga acagccgcgc gatcctgccg cccagcgtgt cgatgtcgaa ctcgacgcgc
     6181 cccgcggtcg cggccaggac gggcgccccg tcccggcaga acacgtcctg gccctcgtgg
     6241 agatggaacc cgccggcgaa ccgcggcgcg tgccacgaat cggtccaggc ggccgggccc
     6301 atgacgacga acggccggta cacgcgccgg gtcgcgacgt ccctgctcat ccccgccttg
     6361 acgagcttgt cgtacacgtc gtcgagcgcc gccgtcgagt acgtgccgcc gatcgacgtc
     6421 ggtgccgttt cggcctgcat ggcgagggtc cgctcgtgcg ccgaacgggc atcgatctca
     6481 tccggcgagg gctgccccgc cggtggcggg gtcagccgct gctctccggg gatcgggagc
     6541 gtcggtgacg acagcggagg ctccgtcgcg gtgggcgccg gcccgggata cgtctcgggg
     6601 ggaggcgtgg gcgtcggcgt cgcgctcgtg tccgtgggcg atggatcgga gggcgcggtc
     6661 gtgttcgtgg gcggtggatc ggaggccggg cccgcatctg tgggcgatgg atcggggggc
     6721 gcgatcgtgt ctgtgggcga cggatcggag ggggggcacg gctcggtcgg cgagggcgtc
     6781 ggcgtcggga tgggctcggt cggcgagggc gtcgccgtcg ggctcggctc ggtcggtgag
     6841 ggcgaggggc tttccgtcga cgcacacgtg gggggcgagg tggtctcggt gggcgtcgga
     6901 tcgatctgtg cgaccgccgg gccccccacg gaaaggccga tggcggccgt cgccgcgagc
     6961 actgcgagga cgccggcacg acgcctgacg gcgatcgctc gtcgccgcgc cgcggtcacg
     7021 cggcgccggt gcggcccgag cacctacccg tcatctgttc ggtcacgctc ctcgacccta
     7081 tcgcgtttgc ccgggcaacc tcggggcccc gtacgactcg gcgaggaacg agccgggacg
     7141 ccgaggtgaa tggccgacga accgggtccc tctcggacgg cccggaccac ctggggtggc
     7201 ggcgggaagt tcagccgagt tgacgcaggg gcgtcacgac ttccgttgcg agaagttcct
     7261 gttgctcgtc gcgccgcccg ccgacccaga agttgaggac cgtgaagccg aggtcgagga
     7321 gctcgagccc gcgctcgacg atctcgtcgg gttctccggt gacccgtcgc tcgggatcgt
     7381 cggccggcgg tccgccgatc cgaacgctca cgttgtaggc gtactcgagc gagtccgggt
     7441 cgcgaccggc gtgctcggcc gctcgtcgca cgagctccat cttgggcttg acctgcgacg
     7501 ggggcgccgg gcccatcgag gggatccagc cgtcggccac acgcccggtg agctcgagcg
     7561 ccttcctccc gtacgtcccg agccagatcg ggatgcggtg ggagggtttg ggctcgagca
     7621 tcgccgcctc gctgcggtag tgacggccgt ggtacgtgaa gggcgactcg gcccagacgc
     7681 cgcggatcat cgcgactcct tcctcgagcg cctcgacctt ctcggaagga gttcgcacgg
     7741 gagcgccgaa cccggcgaat tcctcgtcgt tgcctcctcc tccgagaccg aggatcagtc
     7801 ggccgtccga gagccggtcg agcgtctcgg ccatcttcgc cgtgagcgcg ggcaggcgga
     7861 acggaaggcc gaggacgttc gaggcgacgt ggatcctgct cgttcgggcg gcgatccacg
     7921 tcatcaacgt ccacgtctcc aacgaggggt tctccccgtg agggtggtcc catatcgaga
     7981 cgacgtcgaa accgatctcc tcggcccgtc gtgcctgggc gacgggatcg gagcggcccg
     8041 acgccgaagg gatgatcgag acgccgaacc gcggcatcgg aacctcctgg agctccggct
     8101 cgccggcccc ggatgcgggg gcccgcgtag actgagccca ccgtaaccaa cacggaaggc
     8161 atgggtttgg ctccgtccgt catcgtttcg ggtgctcgaa cgccgatcgg gaagttccgc
     8221 ggcgccttcg ccgggatgtc ggcggtggac ctcggcgcga tcgcgat
//
LOCUS       DATBRK010000376         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1231552, whole genome shotgun sequence.
ACCESSION   DATBRK010000376 DATBRK010000000
VERSION     DATBRK010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231552"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VIH70_11125"
     CDS             <1..514
                     /locus_tag="VIH70_11125"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY8116955.1"
                     /translation="VDVGYTTVDTPVGTLFVASTGRGLVRLAFPEEPFEHVLDELADT
                     LSPRLLEDRTRTDAARRQLEAYFEGRVHRFRTAVDWSLTPPGFSRRVLEVTAAIPFGS
                     VSTYGDVAKRAGSPRAARAAGNALHDNPIPIVVPCHRVVPSGGGVGKYGGQEWRKEFL
                     LDLEGALAGP"
     gene            554..1114
                     /locus_tag="VIH70_11130"
     CDS             554..1114
                     /locus_tag="VIH70_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116956.1"
                     /translation="MSSLFERSAETLDRSRSGDAFGPTPGAAPEPEPTALASIALDDD
                     AARRWLTGAQRPDGSFGLTAGPVENDAPTGLAALALPPGDARERALDHLVATPARTLP
                     PDPVVPHDPNVPGWGWALGTAGWVEPTARALLAFRRLRPGATTQIDGAVGFLADRECV
                     DAGGTSATDPSTTSTSRRSVRRPRPA"
     gene            1153..1377
                     /locus_tag="VIH70_11135"
     CDS             1153..1377
                     /locus_tag="VIH70_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116957.1"
                     /translation="MREVDALRGLCQIERGPLTLAMSLAAFRLLDLADAEEVEHAVDR
                     TLDGPPPDVISLARIALASGPGLSELKVTG"
     gene            1383..>2384
                     /locus_tag="VIH70_11140"
     CDS             1383..>2384
                     /locus_tag="VIH70_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEY8116958.1"
                     /translation="MDRRTFLRRAGIVVAGSAAVGLGAGAAIPSLLHRFDRPWDGDSF
                     APPGRPRVLVARAASYDVDLESIVEEGLAEVGGSLAGRSVLLKPNLVEYDPGTVINTD
                     PRLVAATMLAARRLGASSVLVAEGPGHRRDTPFVVSASGLGERLSEVGARFVDLNLAP
                     VTRVPLHSRFTDLGELWLPAPVVEADVVISMPKMKTHHWAGATLSMKNCFGCMPGRIY
                     GWPKNVLHWAGLQEAIVDVASAVRPELQIVDGIVGMEGNGPIDGTPVQAGVLVFGTDP
                     VATDSTAALVMGLDPAGIWHVNEAGRFLGQADPELIVQVGEDPERSMTSFDVLPVFED
                     "
BASE COUNT          290 a          874 c          869 g          351 t
ORIGIN      
        1 cgtggacgtc gggtacacga cggtcgacac gccggtcggg acgctgttcg tcgcgagcac
       61 gggccggggg ctggtgcggc tcgcgttccc cgaggagccg ttcgagcacg tcctggacga
      121 gctcgccgac acgctctccc cacggctgct cgaggatcgc acgcgcacgg acgccgcgcg
      181 tcgacagctc gaggcctact tcgagggtcg ggtgcaccgg ttccggacgg cggtcgactg
      241 gtcgctcacg ccgccggggt tctcccggcg cgtcctcgag gtgacggccg cgatcccgtt
      301 cgggtcggtc tccacctacg gggacgtcgc caagcgcgcg ggcagccctc gcgcggcccg
      361 ggcggccggg aacgccctgc acgacaaccc gatcccgatc gtcgtgccgt gccaccgcgt
      421 ggtgccctcg ggcggcgggg tgggcaagta cggcggccag gagtggcgca aggagttcct
      481 gctggacctc gagggtgcgc tcgcgggacc ctaggtcctt tggccgagcc tcgggggcgc
      541 cgatactcct gccgtgagct ccctgttcga acgttcggcc gagaccctgg accgctcgag
      601 gagcggcgac gcgttcggtc cgacgcccgg cgccgcgccc gagcccgagc cgacggccct
      661 ggcctcgatc gcgctggacg acgacgccgc gcgccgctgg ctgacgggcg cgcagcggcc
      721 ggacggttcg ttcggcctga ccgcggggcc cgtcgagaac gacgccccta ccgggctcgc
      781 ggcgctcgcc ctcccaccgg gcgacgcacg cgaacgagcg ctcgaccacc tcgtcgccac
      841 cccggcgcgc acgctgcccc ccgaccccgt ggtcccgcac gacccgaacg tcccgggctg
      901 gggctgggcg ctcgggaccg ccgggtgggt ggagccgacg gcacgagccc tcctcgcgtt
      961 ccgcaggctg cggcccggcg cgacgaccca gatcgacggc gccgtcgggt tcctggccga
     1021 ccgcgaatgt gtcgacgcgg gtggaacttc ggcaaccgat ccgtctacta cgtcgacctc
     1081 ccgccgttcg gtcagacgac cgcggccggc ctgatcgggc tgcacgccct cgcgtcgggc
     1141 gacggactcg tcttgcggga agttgatgcg ctccgcggtc tgtgtcagat cgagcgcggt
     1201 cccctcacgc tggcgatgtc gctcgccgcg ttccgcctcc tcgacctggc cgacgccgag
     1261 gaggtcgagc acgccgtcga tcgcacgctg gacggtccgc cgccggacgt gatctcgctt
     1321 gcgcggatcg ccctggcgtc ggggccgggg ctctcggagc tgaaggtgac cggatgacgc
     1381 cgatggaccg ccgcacgttc ctcaggaggg ccggcatcgt cgtcgccggc tcggcggccg
     1441 tgggactggg tgccggcgcg gcgatcccga gcctgctcca tcggttcgac cgtccctggg
     1501 acggcgactc cttcgcgccg cccgggcggc cgagggtcct cgtggcgcga gccgcctcgt
     1561 acgacgtcga cctggagagc atcgtcgagg agggcctggc cgaggtcggc ggttccctcg
     1621 ccggccgttc ggtgttgctc aagcccaacc tcgtcgagta cgacccgggc accgtcatca
     1681 acacggaccc caggctcgtc gccgccacca tgctggcggc gcgacgcctc ggcgcgtcct
     1741 cggtgctcgt ggccgagggt ccggggcacc ggcgcgacac ccccttcgtg gtctcggcct
     1801 ccggtctggg cgaacgcctc tccgaggtcg gggctcggtt cgtggacctg aacctcgccc
     1861 cggtcacccg cgtcccgctc cactcgcggt tcaccgacct gggcgagctg tggctgcccg
     1921 cccccgtcgt cgaggccgac gtcgtgatct cgatgccgaa gatgaagacg caccactggg
     1981 cgggggccac gctgtcgatg aagaactgct tcgggtgcat gccgggtcgc atctacggct
     2041 ggccgaagaa cgtgctgcac tgggccggcc tgcaggaggc gatcgtcgac gtggcctcgg
     2101 ccgtacgtcc cgagctgcag atcgtcgacg ggatcgtcgg catggaaggc aacggcccga
     2161 tcgacgggac gcccgtgcag gccggcgtgc tcgtgttcgg gacggatcct gtggccaccg
     2221 actcgaccgc ggcgctggtg atgggcctgg acccggcggg gatctggcac gtcaacgagg
     2281 ccggcaggtt cctcggccag gccgatcccg agctgatcgt gcaggtcggc gaggatcccg
     2341 aacgctcgat gacctcgttc gacgtgctgc cggtcttcga ggac
//
LOCUS       DATBRK010000377         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1232131, whole genome shotgun sequence.
ACCESSION   DATBRK010000377 DATBRK010000000
VERSION     DATBRK010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232131"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VIH70_11145"
     CDS             <1..250
                     /locus_tag="VIH70_11145"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEY8116959.1"
                     /translation="ADVPSQIATAAEIRAPHRLTHAAQDLAARFHRFYAECPVLSDDA
                     ELTQARLWLCRGTKRTIANLLGMRGVSAPEAMERSDVT"
     gene            314..1606
                     /locus_tag="VIH70_11150"
     CDS             314..1606
                     /locus_tag="VIH70_11150"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY8116960.1"
                     /translation="MSERVLRVGMLGCGTVGAAVVRLLDRHRDDLERRAGCRLEVHKV
                     AVRDPAKPRDVGPLHPAFVDDPMAVVDDPDVDIVCELIGGLEPAGSLVLAAFERDKPV
                     VTANKELLATRGRELFDASDAKGLDLYFEAAVGGGIPLIRPLKESLTGERLRRVMGIV
                     NGTTNFILTKMSEEGRGFADVLADAKRLGYAESDPSADVDGHDAAAKCAILASIAFNA
                     RVVASDVYREGIANVTIEDIEYARRLGYVVKLLAIAEMGEDERIAARVHAAMIPESHP
                     LASVRDAHNAVFVEGPNVGELMFYGRGAGGEATATAVVGDLVSVARNQLAGARGLGCT
                     CFYERTIRPMAEMTGQYYILLRVEDRPGVLAEIASVFGRSEVSIKSVWQEGFGDDAQL
                     VFITHRAREGAFQGAIDVLRGLPGVEEVRSVLRVEAEE"
     gene            1609..2661
                     /gene="thrC"
                     /locus_tag="VIH70_11155"
     CDS             1609..2661
                     /gene="thrC"
                     /locus_tag="VIH70_11155"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY8116961.1"
                     /translation="MARTWRGVIEEFRDRLPVGPATPVVTLGEGGTPLVRSESLSVET
                     GCEVFLKCEGANPTGSFKDRGMTVAISKAVEEGAKAVVCASTGNTSASAAAYAGKAGL
                     TCAVLVPRGKIAPGKMAATLVHGARVLEVEGNFDASFELARGLAERYPVTLVNSVNPY
                     RLQGQKTCAFEIADALGRAPDLHCVPVGNAGNISSHWLGYSEYLADGTIHEPPRLFGF
                     QASGAAPLVLGRPVEDPHTIATAIRIGNPASWDLAIAAASESEGAILSVTDRDILDAY
                     RRIAREGLFAEPASAASVAGLLRLAGEDRLPRGGTVVCVLTGHGLKDPEWAISGGAAP
                     TTVPAEADAVAAELGL"
     gene            2664..3524
                     /gene="thrB"
                     /locus_tag="VIH70_11160"
     CDS             2664..3524
                     /gene="thrB"
                     /locus_tag="VIH70_11160"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY8116962.1"
                     /translation="MRLTATVPATSANLGPGFDCFGLALDLCNEVSLDTDAEPGVTWE
                     GEGADELPTDGTDLVSRAVAETAARFERIVPPASIHCRNRIPLARGLGSSAAAAVAGV
                     ALADAVLGLGLDRTAILEISAGLEGHPDNAAAAVFGGFTIVVDHTVVARFDPDPELRP
                     ALFVPAGIAVSTEAARRALPDSVEREDAVSNAGHAALVAVALLHEPDLLHVAMRDRLH
                     EDVRLGLVPEMRQVIDRLRAAGLAACVSGSGPTLLAFERHDVVTPEPAEGWRIVRVPV
                     RAAGVDIRQD"
     gene            3557..>4759
                     /locus_tag="VIH70_11165"
     CDS             3557..>4759
                     /locus_tag="VIH70_11165"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEY8116963.1"
                     /translation="MTEPDPGLAPAPAADQAANVPLAVVAPTRVPKPEQGAAPVLVTD
                     LASKTLAVKEGQTFLYSDLEGNLDHGGHEGLGLYSHDTRFLSHFRLTVNGRELVLLSS
                     SAERGYQSHVDLTNPDLFEDDVLCAPQQTINVRRVRAISGDLFERIRIKNYNPFDVTI
                     EVGLTFGADFADIFDVRGMTHRHSGGSIPPTVDGSTIEFVHEGRDHVRRITRIRFGID
                     PGRVDVEGAFATAFFRLHLDPYETRLVTVSVEPILGSEPTDAAPDFDHAVHELRRSYE
                     EWERESTRIVTDNELFNQLLDRSLRDLRALYTRVGEGAILAAGIPWYVTIFGRDSLIA
                     SHQLLTVNTRPARDALELLAARQGTEENDWRDEQPGKILHEVRDGELARAGIMPHSPY
                     YGSVDSTPW"
BASE COUNT          670 a         1697 c         1728 g          664 t
ORIGIN      
        1 cgcggacgtg ccgtcccaga tcgcgaccgc cgccgagatc cgggcgccgc accggctcac
       61 ccacgccgcc caggatctcg cggcccgctt ccatcgcttc tacgcggagt gcccggtcct
      121 gtccgatgac gcggagctga cgcaggcccg cctgtggctc tgccgcggca cgaagcggac
      181 gatcgcgaac ctgctcggca tgcgcggcgt gtcggcgccc gaggcgatgg agcgaagcga
      241 cgtgacgtga ggttcgcgcc gggccggggc gacatactga ggcgggccaa gatcgagggg
      301 cgacaggggt gggatgagcg agcgggtcct gcgggtcggg atgctgggct gcggcaccgt
      361 cggcgccgcg gtcgtccggc tgctcgatcg tcatcgcgac gacctcgagc gccgcgccgg
      421 atgcaggctg gaggtgcaca aggtggccgt ccgcgacccg gcgaagccgc gggacgtcgg
      481 gcccctgcac ccggcgttcg tcgacgaccc gatggccgtg gtcgacgatc ccgacgtcga
      541 catcgtctgc gagctgatcg gcgggctcga acccgcgggg tcgctggtcc tcgcggcgtt
      601 cgaacgcgac aagcccgtcg tcaccgccaa caaggagctg ctcgcgacgc gcgggcgcga
      661 gctcttcgac gcctccgacg ccaagggcct ggacctgtac ttcgaggcgg ccgtgggggg
      721 cgggatcccg ctgatccgcc cgctgaagga gtcgctcacc ggcgagcggc tgcgccgcgt
      781 gatggggatc gtgaacggca cgaccaactt catcctgacg aagatgtcgg aggagggacg
      841 tggtttcgcc gacgtcctcg ccgacgcgaa gcggctcggc tacgccgagt ccgacccgag
      901 cgcggacgtc gacgggcacg acgcggccgc caagtgcgcg atcctcgcct cgatcgcgtt
      961 caacgcccgg gtcgtcgcga gcgacgtgta ccgcgagggc atcgcgaacg tgaccatcga
     1021 ggacatcgag tacgcgcgcc ggctcggcta cgtggtgaag ctgctcgcga tcgccgagat
     1081 gggtgaggac gagcggatcg ccgcccgcgt gcatgccgcg atgatccccg agtcgcaccc
     1141 gctcgcgtcg gtccgggacg cccacaacgc ggtcttcgtc gagggcccga acgtcggcga
     1201 gctgatgttc tacggccgcg gcgccggcgg ggaggcgacg gcaaccgccg tagtgggcga
     1261 cctcgtgagc gtcgcgcgga accagctcgc cggtgcccgg ggtctgggct gcacgtgctt
     1321 ctacgagcgg acgatccgtc cgatggccga gatgacgggc cagtactaca tcctgctgcg
     1381 cgtcgaggat cgccccggcg tcctggccga gatcgcgagc gtgttcggcc gcagcgaggt
     1441 ctcgatcaag agcgtgtggc aggaggggtt cggcgacgac gcgcagctcg tgttcatcac
     1501 ccaccgagcc cgcgagggcg cgttccaggg ggcgatcgac gtgctgcgcg gactgccggg
     1561 ggtcgaagag gtccgcagcg tcctccgggt cgaggccgag gagtgacggt ggcccggacc
     1621 tggcgcggcg tgatcgagga gttccgcgac cggctgccgg tcggcccggc gacgccggtc
     1681 gtgacgctcg gcgagggcgg gacgccgctc gtccgctcgg agtccttgtc ggtcgagacc
     1741 gggtgcgagg tcttcctgaa gtgcgagggc gcgaacccga ccggctcgtt caaggatcgt
     1801 gggatgaccg tcgcgatcag caaggcggtc gaggagggcg ccaaggccgt cgtgtgcgcc
     1861 tccacgggga acacgagcgc gagcgccgcc gcctacgcgg gcaaggcggg gctgacctgc
     1921 gccgtcctcg tccccagggg caagatcgcg cccgggaaga tggccgcgac cctcgtgcac
     1981 ggcgcgcgtg ttctggaggt ggaggggaac ttcgacgcct cgttcgagct cgcccgcggc
     2041 cttgccgagc gctacccggt gaccctcgtg aactcggtca acccctaccg cctgcaggga
     2101 cagaagacgt gcgcgttcga gatcgcggat gcgctcggtc gtgcgcccga cctccactgc
     2161 gtcccggtcg gcaacgccgg caacatctcg agccactggc tgggctacag cgagtacctg
     2221 gccgacggga cgatccacga gccaccgcgg ctgttcgggt tccaggcgag cggcgccgcg
     2281 cccctcgtcc tcggtcgccc cgtcgaggac ccgcacacga tcgccaccgc catccggatc
     2341 gggaaccccg cgtcgtggga cctggcgatc gcggccgcgt ctgagtcgga gggcgcgatc
     2401 ctgtcggtga ccgaccgcga catcctggac gcctaccggc ggatcgcgcg cgagggactg
     2461 ttcgccgagc ctgcctcggc ggcgagcgtc gcggggctgc tccggctcgc gggcgaggac
     2521 cggctcccgc ggggcgggac cgtcgtctgc gtgctcacgg gtcacgggct gaaggacccg
     2581 gaatgggcga tcagcggggg ggccgcgccg accacggtcc cggccgaggc ggacgccgtc
     2641 gcggccgagc tcgggctgta gccgtgcgcc tcaccgcgac ggtcccggcg acgtccgcga
     2701 acctgggacc cggattcgac tgcttcgggc tcgcgctcga tctctgcaac gaggtctcgc
     2761 tggacacgga cgccgagccc ggcgtgacct gggagggcga gggcgcggac gagctgccga
     2821 ccgacgggac cgacctcgtg tcgcgcgccg tggccgagac cgccgcgcgc ttcgaacgca
     2881 tcgtcccccc cgcctcgatc cactgccgca accggatccc gctcgcacgt ggcctggggt
     2941 cctcggccgc agccgcggtc gccggcgtgg cgctggccga cgcggtgctc ggactcggtc
     3001 tggaccggac cgcgatcctc gagatctccg cggggctgga gggccatccc gacaacgccg
     3061 ccgccgccgt gttcggcggc ttcacgatcg tcgtggacca caccgtcgtg gcccgcttcg
     3121 accccgaccc cgagctgcgg ccggcgctgt tcgtcccggc aggcatcgcg gtctcgaccg
     3181 aggcggccag gcgcgcgctc ccggactcgg tcgagcgcga ggacgccgtg tccaacgcgg
     3241 gacacgccgc cctcgtggcg gtcgccctcc tgcacgagcc cgacctcctc cacgtggcca
     3301 tgcgcgatcg cctccacgaa gacgtccggc tggggctcgt cccggagatg cgccaggtca
     3361 tcgaccgcct gcgcgccgcc gggctggcgg cgtgcgtctc gggttcgggg ccgaccctgc
     3421 tcgcgttcga gcgccacgac gtcgtcacgc cggagccggc cgaggggtgg cggatcgtgc
     3481 gtgttcccgt gcgagccgcc ggggtcgaca tccgccagga ctgaaggcgc gtatcacctg
     3541 cgacgatggt tgaacgatga cagaacccga tcctgggctc gccccggcgc ccgctgccga
     3601 ccaggcggcg aacgtcccgc tcgccgtcgt cgcgcccacc cgggtcccga agcccgaaca
     3661 gggcgcggcc ccggtcctgg tcacggatct cgcgagcaag accctcgcgg tcaaggaggg
     3721 ccagaccttc ctgtactccg acctcgaggg caacctcgac cacggcggtc acgaaggtct
     3781 cggcctgtac tcgcacgaca cgcggttcct gtcgcacttc cggctgaccg tgaacggacg
     3841 cgagctcgtg ctgctgtcct cgtcggccga acgcggctac cagtcgcacg tcgacctgac
     3901 gaaccccgac ctgttcgagg acgacgtgct ctgtgcgccg cagcagacga tcaacgtccg
     3961 gcgcgtccgc gcgatctccg gagatctgtt cgagcgtatc cggatcaaga actacaaccc
     4021 cttcgacgtc accatcgagg tcgggctgac gttcggcgcc gacttcgccg acatcttcga
     4081 cgtgcgcggc atgacccatc gccactcggg cgggtcgatc ccgccgacgg tggacggctc
     4141 cacgatcgag ttcgtgcacg aggggcgcga ccacgtccgg cggatcactc ggatccgctt
     4201 cggcatcgac cccggccgcg tggatgtcga gggggcgttc gccacggcgt tcttccggct
     4261 ccacctcgat ccgtacgaga cccggctcgt gaccgtctcg gtcgagccga tcctggggag
     4321 cgagccgacc gacgcggccc cggacttcga ccacgcggtg cacgagctcc gccgctccta
     4381 cgaggagtgg gagcgcgagt cgacgcggat cgtcaccgac aacgaactgt tcaaccagct
     4441 cctcgaccgg agcttgcgcg atctgcgcgc gctgtacacg cgggtggggg agggcgcgat
     4501 cctcgccgcg gggatcccgt ggtacgtgac gatcttcggc cgcgactccc tgatcgcgtc
     4561 gcaccagctg ctgacggtga acacgcgacc agcccgcgac gcgctcgagc tcctggcggc
     4621 gcgtcagggc acggaggaga acgactggcg tgacgagcag ccaggcaaga tcctgcacga
     4681 ggtccgcgac ggcgagctcg cgcgcgcggg gatcatgccg cactcgccgt actacgggtc
     4741 cgtcgactcg acgccgtgg
//
LOCUS       DATBRK010000378         6598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2528930, whole genome shotgun sequence.
ACCESSION   DATBRK010000378 DATBRK010000000
VERSION     DATBRK010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2528930"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VIH70_11170"
     CDS             complement(<1..1101)
                     /locus_tag="VIH70_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEY8116964.1"
                     /translation="MPSSTSQQLDVDALIVGAGPGGSTAAYHLARQGLDVVMVEKAAF
                     PREKVCGDGLTPRAVVQLRRLGIDTDDPRFERHEGLRIYSRRVTLELPWPDLEAFPNF
                     GLMMTRAELDELLARHAEKAGARLMERTEAVAPTQQDGWVTGARIRGQDDRETTVRAR
                     FVIAADGASSRFASQAGVRRDSGKPLGIAARRYYRVERRPGPWLEVWMDLREADDTIL
                     PGYGWLFPLADGSINVGAGLLNTFRDFKDLSAQRVFDAFWRMLPAEWNVNESSADGRV
                     LSGPLPMGFSRTPPATPGMLVVGDAAGLVNPFNGEGIAYAMEAGELAAELIHEALVKD
                     RPGMAHLYPTELRERYGRYYRIGNVFVRAIGHP"
     gene            complement(1226..1669)
                     /locus_tag="VIH70_11175"
     CDS             complement(1226..1669)
                     /locus_tag="VIH70_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8116965.1"
                     /translation="MRVSHAELGAARRMFASLPSAAVATMNADGSPHVVPLWFVWPED
                     AIFVSTRQEGRTWANVVRDPRLAIAIDLGRAWVEVAGVEILGRAEPLSAESAAMRKPI
                     SAWHEKYRPLLAGEGFARFAEEVRGLGFLRVIPDTVNAWDHARGR"
     gene            complement(1669..2547)
                     /locus_tag="VIH70_11180"
     CDS             complement(1669..2547)
                     /locus_tag="VIH70_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8116966.1"
                     /translation="MPPKDRTAPAASPPAIDAVRGPVDRELVRFLGDRRAELAGMDPS
                     AVALVDELLRLLGAGGKRVRPALCFWAFRAAGGRKDDPIVRVCGALELLHTSALVHDD
                     LMDRDVERRGVASAHVRFAEGAPAGVDPDGFGTSAAILVGDLALVLSERMIRTSGFGA
                     VRLEPAMTRFDRMRVEMAAGQFLDVSGEGDRGRVRALKTGSYTAEGPVMIGAALAEAG
                     PQAEGPLRVFGRLVGEAFQLRDDVADGDAANDAADEVDELVLRAERALGDAPLRREGV
                     DALIEIAELLRAPGTA"
     gene            2632..4440
                     /locus_tag="VIH70_11185"
     CDS             2632..4440
                     /locus_tag="VIH70_11185"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY8116967.1"
                     /translation="MTRFQKLTIATTASTVLLIAAGGLVRATGSGLGCPGWPTCFGRW
                     IPPLETHAIIEYSHRLLATIAVVLICLQAVVAWRQYRRVRQILVPSLAAVGLVFVQAA
                     LGGVVVRGELEATLVTLHFATAMTLLAVLVNITANSSCYVRLPLKGPSIAGSDPRFAR
                     LTLWTAAAAFALLIVGASVRAEAAGLAFRDWPLMDGRLVPTLGGVATLMFVHRVLAAL
                     VLLLVIWVAIRARTMTHRSKDLVILSSVTLLLYVAQIFVGAANVWSSLSAASVTAHVA
                     LSALIWGVLVAIATVSRRFAGHAREAEEGDARSNGKRSNLRETTAAYFRLTKPRIIVL
                     LLITTVPAMVLAQRGVPSLGLIVATLVGGTIAAGSANAINCYLDRDIDEVMRRTRTRP
                     LPAHQVEPDGALAFGFVLGAISFFFLSIAVNVLAASLALSAIAFYVFVYTMWLKRTSV
                     QNIVIGGAAGAVPALVGWAAVAGTVGPAAWVLFAIVFVWTPPHFWALAMRYSGDYRAA
                     GIPMLPVVRGPQETRKQILLYSLVLFGTSLLLVPVASMGLVYTGAAVLLGGRFVWHAL
                     RLWRGTSPAESMRLFRFSIVYLALLFAAVAADALVG"
     gene            4524..4715
                     /locus_tag="VIH70_11190"
     CDS             4524..4715
                     /locus_tag="VIH70_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116968.1"
                     /translation="MIGFMVVLFLVVVVGSFPATWLLMLFLGNVGVNVSFWGALPAGI
                     LMTFFVAGTGGLSRYRSAS"
     gene            complement(4814..>6598)
                     /locus_tag="VIH70_11195"
     CDS             complement(4814..>6598)
                     /locus_tag="VIH70_11195"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY8116969.1"
                     /translation="SAALVHLAFGSPGGLPSMEQVRAALEELGVAAVDLRVAELQPKG
                     VALLRAETPQGRPLVVKVYGRDAWDGQLLTSTWSYLWYRDEAPTLTISRRQQVEHEAF
                     ITLLAERSRVPVLPVIAAGVAGARDGVLVLEAEGRPIQQLAPDDVTDALLADLWRAVT
                     VMQEAGIAHGALDGQRLFVAGDGSARIGDFQAAAASAQPPAMLADRARLLVTTALLTR
                     DDRWAAAALAGIGADGLTDILPYIQPAALSRFTRRSLKDAGHDLDAIRDRAAAAAGTE
                     PPKLEPLRRVTVGSLLTVALLLLAAYFVFTAIANVGIDQVVEEFTKADTAWLWAGLVV
                     VPIIPVAQAFGTLGATTLPLRLGPVIALEYAIQFIALAVPSSAARVAVNVRFFQRQGA
                     PAAQALTIGLIDSVFGFVVQVILLITIVVSGLVTLDISLDGLNIDPSGKLLVLVGIVV
                     VAAVVVALTVPKIRRPIAARIAEARPALAVIRSPIKLAGLLGGNFVAQFLLALVLGAT
                     VRAFGESATMAELLLTNTLVSLFAGVMPIPGGIGVSEAAIAFCLTAIGIPSSTAAAIA
                     LVFRLLTFYLPPIWGGFGMRWLRRQAYL"
BASE COUNT          935 a         2424 c         2266 g          973 t
ORIGIN      
        1 aggatgcccg atcgccctga cgaacacgtt gccgatccgg tagtagcgcc cgtaccgctc
       61 gcggagctcg gtcgggtaca ggtgcgccat gccgggccgg tccttcacca gcgcctcgtg
      121 gatcagctcc gccgcgagct cgcccgcctc catcgcatag gcgatcccct cgccgttgaa
      181 cgggttgacc aatccggcgg cgtcacccac gacgagcatg ccgggggtcg ccggcggcgt
      241 ccgagagaag cccatcggga gcggccccga cagcacccgc ccgtcggccg agctctcgtt
      301 gacgttccac tcggcgggaa gcatgcgcca gaacgcgtcg aacacccgtt gcgccgacag
      361 gtccttgaag tcacggaacg tgttcagcag gcccgcgccc acgttgatcg agccgtcggc
      421 gagcgggaac agccagccgt atccgggcag gatcgtgtcg tcggcctcgc gaaggtccat
      481 ccagacctcg agccatggtc ccgggcgacg ctcgacgcgg taataccgcc gcgcggcgat
      541 cccgaggggc ttgcccgaat cgcggcgcac gccggcctgc gacgcgaacc gcgacgaggc
      601 gccgtccgcc gcgatcacga accgggcgcg gaccgtcgtc tcgcggtcgt cctggcccct
      661 gatccgagcc ccggtcaccc aaccgtcctg ctgggtcggg gcgacggcct ccgtccgctc
      721 catcaggcgg gcgccggcct tctcggcgtg gcgcgccagg agctcgtcga gctcggcccg
      781 cgtcatcatc aggccgaagt tcgggaaggc ctcgaggtcc ggccacggga gctcgagggt
      841 cacgcgccgc gagtagatcc gcagcccctc gtgccgctcg aaccgcgggt cgtcggtgtc
      901 gatgcccagg cgccgcagct ggacgaccgc gcggggcgtg aggccgtcgc cacagacctt
      961 ctccctgggg aacgcggcct tctcgaccat gaccacgtcc aggccctggc gggcgaggtg
     1021 gtaggccgcg gtggagcctc ccggacccgc gccgacgatc agggcgtcga cgtcaagctg
     1081 ctggctcgtc gaactcggca cgaggtcccc cacctgcccg tgaaaaactt ccttgtgaaa
     1141 actttcacaa aggatgctaa ccgacttgtg aagctccgca caaaccctat gcccgggtct
     1201 tcggcggcgg caagcgagcg catggtcagc gcccgcgggc gtggtcccag gcgtttacgg
     1261 tgtccggaat cacgcggagg aacccgagcc cccggacctc ctcggcgaac cgcgcgaacc
     1321 cctcgccggc gaggaggggc cggtacttct cgtgccaggc cgagatcggc ttgcgcatcg
     1381 ccgcgctctc ggccgaaagc ggttcggccc tgccgaggat ctcgacaccc gcgacctcga
     1441 cccacgcccg gcccaggtcg atcgcgatcg cgagccgggg atcgcggacg acgttggccc
     1501 acgtccggcc ctcctgccgg gtcgagacga agatcgcgtc ctcgggccag acgaaccaga
     1561 gcggcacgac gtggggggat ccgtcggcgt tcatggtcgc gacggcagcc gagggcaggc
     1621 tcgcgaacat gcggcgcgcc gccccgagct ctgcgtgcga cacgcgcatc aggccgtccc
     1681 cggggcgcga aggagctccg cgatctcgat cagcgcgtcg accccctcgc ggcggagcgg
     1741 cgcgtcaccg agcgcccgct cggcgcgcaa cacgagttcg tcgacctcgt cggcggcgtc
     1801 gttcgcggcg tcgccgtcgg ccacgtcgtc ccggagctgg aacgcctccc ccaccagccg
     1861 cccgaacacg cgcaggggac cctcggcctg cggtccggcc tccgcgagcg cggccccgat
     1921 catcaccgga ccctcggcgg tgtacgaccc tgtcttcagc gcgcggaccc ggccccgatc
     1981 cccttcgccc gacacgtcca ggaactgacc cgccgccatc tcgacgcgca tgcgatcgaa
     2041 gcgggtcatc gcgggttcga gccggacggc cccgaacccg ctcgttcgga tcatccgctc
     2101 cgacagcacc agcgcgagat cgccgaccag gatcgcggcc gaggtcccga acccgtcggg
     2161 gtccacaccg gccggcgccc cttcggcgaa ccggacgtgg gccgaggcca cgccgcggcg
     2221 ctcgacgtcc cggtccatca ggtcgtcgtg cacgagcgcg gatgtgtgca ggagctcgag
     2281 cgccccgcag acccgaacga tcggatcgtc ctttcggccc cccgccgctc ggaacgccca
     2341 gaagcacagc gctggccgga cccgcttgcc gccggcgccg agcaggcgga gcagctcgtc
     2401 gaccagcgcg accgcggacg gatccatccc cgcgagctcc gcgcggcggt cgccgaggaa
     2461 ccgaacgagc tcccggtcca ccggcccccg tacggcgtcg atcgccggcg gcgacgcggc
     2521 gggcgcggtg cggtccttcg gcggcaccca ggcagtatgc caacgcacgg ggtcgtcacc
     2581 ccgcccggcg ggaggggtgc tgctatcgtt cggacgagcc cggctcgaac gatgacgcgc
     2641 ttccagaagc tgacgatcgc cacgaccgct tcgacggtcc ttctcatcgc ggccgggggg
     2701 ctcgttcgag cgacgggttc ggggctcggc tgcccggggt ggcccacgtg cttcggacgg
     2761 tggatcccgc cgctggagac ccacgcgatc atcgagtact cccaccggct gctcgcgacg
     2821 atcgcggtgg tcctgatctg cctgcaggcc gtcgtggcgt ggcggcagta ccggcgcgtc
     2881 cgtcagatcc tggtgccgag cctggccgcg gtcggcctcg tcttcgtgca ggcggcgctc
     2941 ggcggcgtcg tcgtgagggg cgagctcgag gcgacgctcg tgacgctgca cttcgcgact
     3001 gcgatgacgc tgctcgcggt cctggtgaac atcaccgcga actcctcctg ctacgtgagg
     3061 ctgccgctca aggggccgtc gatcgcgggc tcggatccga ggttcgcccg gctgacgctc
     3121 tggacggccg cggccgcgtt cgccctgctg atcgtgggcg cctcggtccg cgccgaggcg
     3181 gccggcctcg cgttccggga ctggccgctg atggacggac ggctcgtgcc gacgctcggc
     3241 ggcgtcgcga ccctgatgtt cgtgcaccgt gtgctcgccg ctctggtcct gctgctcgtg
     3301 atctgggtcg cgatccgggc ccggacgatg acgcaccgct cgaaggatct cgtgatcctg
     3361 tcatcggtca cgttgctgct ctacgtcgcg cagatcttcg tgggcgcggc caacgtgtgg
     3421 tcgagcctgt cggccgcgtc tgtcaccgcg cacgtcgccc tgtccgcgtt gatctggggc
     3481 gtcctggtcg cgatcgccac cgtcagccgc cgcttcgcgg gtcacgcgcg tgaggccgag
     3541 gagggcgacg cgcgctccaa cggcaagcgc tcgaacctgc gcgagacgac cgcggcgtac
     3601 ttcaggctca cgaagccgcg catcatcgtc ctcctgttga tcacgacggt cccggcgatg
     3661 gtgctcgccc agcggggagt gccgtcgctc ggtctgatcg tcgcgacgct ggtgggcggc
     3721 acgatcgccg cgggctcggc gaacgcgatc aactgctacc tcgaccgcga catcgacgag
     3781 gtgatgcgcc ggacccgcac gcggccgctc cccgcccacc aggtcgagcc ggacggcgcg
     3841 ctcgcgttcg ggttcgtgct gggcgcgatc tccttcttct tcctctcgat cgcggtgaac
     3901 gtgctcgcgg ccagcctcgc gctgtcggcg atcgcgttct acgtgttcgt gtacaccatg
     3961 tggctcaagc gcacgagcgt gcagaacatc gtgatcggcg gggccgccgg agcggtcccg
     4021 gcgctggtcg gctgggcggc ggtcgccggg accgttggtc cggcggcgtg ggtgctgttc
     4081 gcgatcgtct tcgtctggac accgccgcac ttctgggccc tcgcgatgcg ctacagcggg
     4141 gactaccgcg cggcgggcat cccgatgctg ccggtggtcc gggggccgca ggagactcgc
     4201 aaacagatcc tgctctactc cctggtgctg ttcggcacgt ccctgctgct cgtgccggtc
     4261 gcctcgatgg gcctcgtcta cacgggcgcg gccgtcctgc tcggcggccg gttcgtgtgg
     4321 catgcgctcc gactgtggcg cgggacctcg ccggccgagt cgatgcgtct gttccgcttc
     4381 tcgatcgtct acctcgccct gctgttcgcc gcggtcgccg ccgacgcgct cgtcggctaa
     4441 tgggactcta gacccgcccc acctacccgc acggaccgtc acgccgtgcg acgatctcgc
     4501 caccagcgct gaggaggtcc aagatgatcg ggttcatggt cgtgctgttc ctcgtcgtcg
     4561 tcgttggatc gttcccggcg acctggctcc tgatgctgtt cctcggcaac gtcggcgtga
     4621 acgtgagctt ttggggagcc ctgcccgcag ggatcctgat gacgttcttc gtcgccggga
     4681 ccggtggcct cagccggtac cgctcggctt cctaggcttc ggactccgac cgaccggtcg
     4741 ggcttcgccg tgcccaggcg caacgggacc gcgccctcga gctcgatctc gagagacgag
     4801 ctcagctgta gtgctacagg tacgcctgtc tgcggagcca gcgcatcccg aacccgcccc
     4861 agatcggcgg taggtagaag gtcagaagac ggaacacgag cgcgatcgcc gcggccgtcg
     4921 agctcgggat cccgatggct gtgaggcaga aggcgatcgc cgcctcgctc acgccgatcc
     4981 cgcccgggat cggcatcacc ccggcgaaca ggctgacgag cgtgttcgtg agcaggagct
     5041 cggccatcgt cgcgctctcg ccgaaggctc gtacggtcgc tcccagcacg agagccagca
     5101 ggaactgcgc aacgaagttg ccgccgagca gcccggccag cttgatcggc gagcggatca
     5161 cggccagcgc cggccgagcc tcggcgatcc tggccgcgat cggccggcgg atcttcggca
     5221 ccgtgagggc gaccactacc gcggcgacca ccacgatccc gacgagcacg aggagctttc
     5281 cgctcgggtc gatgttcagg ccgtcgagcg agatgtcgag ggtcacgagc cccgagacca
     5341 cgatcgtgat cagcaagatc acctggacga cgaacccgaa cacgctgtcg atcagtccga
     5401 tcgtcagtgc ctgggccgcc ggcgccccct gccgctggaa gaagcggacg ttcacggcga
     5461 cccgcgcggc cgagctcggc acggcgagcg cgatgaactg gatcgcgtac tcgagcgcga
     5521 tgaccggacc aaggcgcagg ggcagcgtgg tcgcgccgag ggtcccgaac gcctgggcca
     5581 ccgggatgat cggaaccacg accaggcccg cccacaacca tgcggtatcg gccttcgtga
     5641 actcctccac gacctggtcg atgccgacgt tcgcgatcgc cgtgaagacg aagtacgcgg
     5701 ccagcagcag gagcgcgacc gtcagcagcg agccgaccgt gaccctcctg agcggctcga
     5761 gcttcggcgg ctccgttccc gccgcggccg cggctcggtc tcggatcgcg tccaggtcgt
     5821 gcccggcgtc cttgagcgat cgccgggtga agcggctcaa ggcggcgggt tggatatacg
     5881 gcaggatgtc ggtcagccca tctgcgccga tcccagcgag ggcggccgcc gcccagcgat
     5941 cgtcgcgggt gagcaacgcc gtcgtcacga gcaatcgcgc ccggtcggcg agcatcgccg
     6001 gcggctgggc gctcgcggcc gcggcctgga agtccccgat ccgggccgac ccgtcaccgg
     6061 cgacgaacag gcgctgcccg tccagggcac cgtgcgcgat gccggcctcc tgcatcacgg
     6121 tcaccgcgcg ccacaggtcg gcgagcaacg catcggtcac gtcgtccggc gcaagctgct
     6181 ggatcggtcg tccctcggcc tccaggacga gcaccccgtc acgggcgccc gcgacgcccg
     6241 cggcgatgac cggcagcacc ggcacccgcg accgctcggc gagcagcgtg atgaacgcct
     6301 cgtgctccac ctgctgtcgg cgactgatcg tgagcgtcgg cgcctcgtcc cggtaccaca
     6361 gatacgacca cgtcgacgtg agcagctgac cgtcccaggc atcgcgccca tagaccttga
     6421 cgacgagcgg ccgtccctgc ggcgtttcgg cgcgcaggag cgcgacgccc ttgggttgaa
     6481 gctcggccac acgcagatcg accgccgcca ccccgagctc ctcgagcgcc gcacggacct
     6541 gctccatcga gggcaggccc cccggggatc cgaacgccag gtgcaccaac gccgccga
//
LOCUS       DATBRK010000379         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1232701, whole genome shotgun sequence.
ACCESSION   DATBRK010000379 DATBRK010000000
VERSION     DATBRK010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232701"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VIH70_11200"
     CDS             <1..1035
                     /locus_tag="VIH70_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY8116970.1"
                     /translation="HNAITDVDGVGVGHATVVRDQPSVARTGVTAIWPHPGDPWRERV
                     YAGTAIVNGYGELIGIDQINEWGLLHSPVVITSSLAIGLAYDTTAKWIAEREPAQGRS
                     DVIMPVVTECDDSFLNDARAFPLSPADVVAALDGASRGEVAEGCVGAGTGMQCFDFKG
                     GIGTASRVVADAAGDLTVGVLVCTNFGARPDLTIAGVPVGLALTDLMPEGHAAGSCIV
                     VVATDAPMLPHQLQRLALRSGLGLARCGSIGANGSGELMLAFSTAQTVPRAAPGSRIR
                     VEALLDGAFWHEPSAFDPIFTATIEATEEAVVNALFAAETTAGRDGNVLHALPRDRVL
                     EMLDRAGRTE"
     gene            1242..2789
                     /locus_tag="VIH70_11205"
     CDS             1242..2789
                     /locus_tag="VIH70_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8116971.1"
                     /translation="MAARADQKAMISEKVAPVLVARTLGPFDLVVIFVAIVLFINNAA
                     GVQFAGPSVFIIWIVAFATFLITGAFVTAQLGRMFPEEGSLYVWTHKVLGPFWGFFAG
                     FVAWWPGPISLVFIGILFANFLQQFALFAKPSCEFPCIFTENWQIGIVVLAVIWFSAA
                     MSYLKMRVTQNYVNVLFWFYAAAIFLIGFAGVWWLLQGNGSSTDFGAGWNPFKGDELA
                     LGLPANLTFFSFAILALLGIETPLNMGAEVTGGEKSIRTFLLWGCAIVMVAYLWATWG
                     NMIVIEAGGANGTTGGAETVGLALGRWTGAAVSLVLAWVILTVAVVYNYSFGRLLFVS
                     GMEKRLPHQLGKVNRNRVPANAITLQSVIASILAIMIFFVLGSGETDPYKYFYALYAG
                     VTIVWCISTALLFLDIFFAKRAEPERFERERRISVGWLYICGAIGFIVNVLAVLFIFV
                     GSWYPTGFPTLAEWNWWMFGITAVSVLSGVVIYIVSQSARRGRTDEELIELGEAEREI
                     AEDMPRA"
     gene            2811..>3421
                     /locus_tag="VIH70_11210"
     CDS             2811..>3421
                     /locus_tag="VIH70_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY8116972.1"
                     /translation="MTPDEAVAVCRQAGVRLVRFLYCDNGGIVRAKAAHVERLADRMR
                     TGIGLTVAMQAMSSLDQLQPVEGMGPVGEVRLVPDPSTLRILPYAPNSAAMLVDHVRL
                     DGEPYEAGPRSFLKRMDTALTERGMVLRCSVENEFSLATRADGGFAPIDESLCFSTVG
                     MTASQEVTDAIVAALEAQSIQLEQYYVELGHGQQELSVVHLPVL"
BASE COUNT          490 a         1122 c         1202 g          607 t
ORIGIN      
        1 cacaacgcca tcaccgacgt ggacggcgtg ggcgtcggcc acgcaacggt cgtccgtgac
       61 caaccctcgg ttgctcgcac cggcgtcacc gcgatctggc cgcacccggg ggacccttgg
      121 cgggagcgcg tgtacgccgg caccgcgatc gtgaacggct acggcgagct gatcggcatc
      181 gatcagatca acgagtgggg gctcctccac tcgcccgtcg tgatcacctc gagcctcgcg
      241 atcggtctgg cctacgacac gacggccaag tggatcgccg aacgcgaacc ggctcagggt
      301 cgctccgacg tgatcatgcc ggtggtcacc gagtgcgacg actcgttcct caacgacgct
      361 cgggcgttcc cgctctcgcc tgcggacgtc gtcgccgcgc tcgacggtgc ttcgcgaggc
      421 gaggtggcgg aggggtgcgt cggggccgga accgggatgc aatgcttcga cttcaagggc
      481 ggcatcggca cggcgtcacg agtggtggcc gacgcggccg gcgacctgac ggtcggtgtg
      541 ctcgtctgca ccaacttcgg cgcccgcccc gacctgacga tcgcgggcgt gccggtcggc
      601 ctcgcgctca cggatctgat gcccgagggc cacgcggcgg gctcgtgcat cgtggtcgtc
      661 gcgaccgacg cgccgatgct cccgcaccag ctccagcgac tcgccctccg ctcggggttg
      721 gggctcgctc gctgtgggtc gatcggcgcc aacggctcgg gcgagctcat gctggcgttc
      781 tcgacggctc agaccgttcc acgcgcggcg ccagggtcga ggatccgcgt cgaggcgctc
      841 ctggacggcg cgttctggca cgagccgagc gcgttcgatc cgatcttcac ggcgacgatc
      901 gaggcgacgg aggaggcggt cgtgaacgcg ctcttcgcgg ccgagaccac cgcggggcgc
      961 gacgggaacg tcctccacgc gctcccccgc gaccgggtcc tggagatgct ggatcgcgcc
     1021 ggaaggaccg agtgaggcgg ttgcggctgg cgaagatccc ccgagttgcc tccgggcaca
     1081 cctccggggc cgttcggccg caggggtttc gagcgaatat cagccgaaag tcggggttgc
     1141 gcacgccgac cggccgatgc aaggatgggc cgacgacggc tggtgggggg cgcgcccgca
     1201 ccctgtaggt cgtgctcgcc ccccggacgg gagggtgacg catggcagca cgggccgacc
     1261 agaaggccat gatctccgag aaggtcgcgc cggtccttgt ggcgcgaacg ctcgggccat
     1321 tcgacctagt cgtgatcttc gtcgcgatcg tcctgttcat caacaacgcg gccggggtgc
     1381 agttcgccgg cccgtcggtg ttcatcatct ggatcgtcgc cttcgcgacg ttcctgatca
     1441 ccggggcgtt cgtgaccgcc cagctggggc ggatgttccc cgaggagggt tcgctctacg
     1501 tttggaccca caaggtgctg ggcccgttct gggggttctt cgccggcttc gtcgcctggt
     1561 ggcccgggcc gatctcccta gtgttcatcg gcatcctgtt cgcgaacttc ctccagcagt
     1621 tcgctctgtt cgcgaagcca tcttgcgagt tcccgtgcat cttcaccgag aactggcaga
     1681 tcggtatcgt cgtcctcgcc gtgatctggt tcagcgcggc gatgtcctac ctcaagatgc
     1741 gtgtcacgca gaactacgtg aacgtgctct tctggttcta cgcggccgcg atcttcctca
     1801 tcgggttcgc aggcgtgtgg tggctgctgc aggggaacgg gtcctcgacg gacttcgggg
     1861 ccgggtggaa ccccttcaag ggcgatgagc tcgcgttggg tctgcccgcg aacctcacgt
     1921 tcttctcgtt cgcgatcctc gcgctcctcg ggatcgagac gcccctgaac atgggcgccg
     1981 aggtgacggg tggcgagaag tcgatccgca cgttcctgct ttggggctgt gcgatcgtca
     2041 tggtggcgta cctctgggcg acctggggca acatgatcgt gatcgaggcc ggcggggcga
     2101 acgggaccac cggaggcgcc gagacggtcg ggctcgccct cgggaggtgg accggcgccg
     2161 ccgtctcgct ggtgctggct tgggtcatcc tgacggtggc cgtcgtctac aactactcgt
     2221 tcgggcgcct gctgttcgtg tcaggcatgg agaagcggct cccccaccag ttgggcaagg
     2281 tcaaccggaa cagggtgccg gcgaacgcga tcacgctcca gtcagtgatc gcgagcatcc
     2341 tggcaatcat gatcttcttc gtcctgggct cgggcgagac cgacccgtac aaatacttct
     2401 acgcgctgta cgccggcgtc acgatcgtgt ggtgcatctc gaccgcgttg ctgttccttg
     2461 acatcttctt cgccaagcgc gccgagccgg agcggttcga gcgcgagcgc aggatctcgg
     2521 tcggctggct ctacatctgc ggcgcgatcg gattcatcgt caacgtcctg gcggtgctct
     2581 tcatcttcgt gggctcgtgg taccccacgg ggttccccac gctggccgag tggaactggt
     2641 ggatgttcgg catcacggcc gtctcggtcc tgtcaggcgt ggtgatctat atcgtctcgc
     2701 agtccgcaag gaggggcagg accgacgagg agctgatcga gctcggggag gccgagcggg
     2761 agatcgcgga ggacatgccg agggcctgac gagtctcgat ccgacgccgc atgacgccgg
     2821 acgaggcggt cgccgtctgc aggcaggcgg gggtccgcct cgtccggttc ttgtattgcg
     2881 acaacggagg catcgtccgg gccaaggccg cgcacgtgga gcggctggcg gaccggatgc
     2941 ggaccgggat cggcctgacc gtcgcgatgc aggccatgag ctccctcgac cagctccaac
     3001 cggtggaggg gatgggcccg gtgggcgagg tccgtctggt ccccgatccc tcgacgctgc
     3061 gaatcctgcc ctacgcgccg aactcggcgg ccatgctcgt cgaccacgtc cgtctggacg
     3121 gcgagcccta cgaggccggc ccgcggtcct tcctcaaacg catggacacc gcactcaccg
     3181 aacgcgggat ggtcctgcgg tgctcggtgg agaacgagtt cagcctggcg acgcgcgccg
     3241 acggggggtt cgcgccgatc gacgagagcc tctgcttctc gacggtgggg atgaccgcgt
     3301 cgcaggaggt gacggacgcg atcgtcgccg cgctcgaggc gcaatcgatc cagctcgagc
     3361 agtactacgt ggagctcggg cacggccagc aggagctctc ggtcgtgcac ctcccggtgc
     3421 t
//
LOCUS       DATBRK010000380         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_937747, whole genome shotgun sequence.
ACCESSION   DATBRK010000380 DATBRK010000000
VERSION     DATBRK010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937747"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1643
                     /locus_tag="VIH70_11215"
     CDS             147..1643
                     /locus_tag="VIH70_11215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY8116973.1"
                     /translation="MTAVKMPQLGETVIEGTILKWLKQEGDQVALDEPLFEISTDKVD
                     TEVPSSAAGTLTKILVPEGETVAVGTELAEISQGGAGGAEAPGAASQPAAEYSGGAET
                     SSSNAGGDDAPSAPAPPSAAAPAAPAPPAAPAPTPAASPAAASTDTVPSQMAASTQTG
                     LPDRGPRSQILSPLVRKLANEHRVDLSQVSGTGTGGRITKNDVLGFVATQGAAAPATS
                     APAAVSAAQPAQPAPAAASTAEPVQPAPPSAPAAAPAVAQPAPAAMGGGEELVPVSHI
                     RRTIAQHMKASLEISARAWNAVEINMENVARVRERTKDAFKAREGFGLTYMPFVTRAV
                     TEALLAHPMVNSELRGDEIAVKHFVNMGIAVSYDEGLIVPVLKGSDTMNLLGIARGIN
                     DLATRARAKKLLPDEVHGGTFTITNPGPFGSLISVPIINQPQTGILGFDTVEKRPVVI
                     DDTIAIRHMVYISMSWDHRVIDGALASQFLARVKQNLETWDFAEDLAL"
     gene            1648..>2167
                     /gene="lipB"
                     /locus_tag="VIH70_11220"
     CDS             1648..>2167
                     /gene="lipB"
                     /locus_tag="VIH70_11220"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY8116974.1"
                     /translation="MARLIRPAGPVDYDEANERMHALAERRLAGEGEDTLILLEHPPV
                     YTAGRRWKPDHIVWTEDRIRAAGADLRFIDRGGSLTFHGPGQLVGYPVFDLGARPDAM
                     RYVRDLEEVVISAAADVGVALHRSDVQTGVWSGSRKVCAIGVRLMRMRVSLHGFALNC
                     TTDLSWYDAIVPC"
BASE COUNT          338 a          749 c          777 g          303 t
ORIGIN      
        1 gtcgctcgac gcaccggtcg tccggctctc gccgcctcac gtcccggtgc cgttctctcc
       61 gccgctcgag gacgcatacc tgccgcaggt gtccgacatc gaggacgccg tccgcaagct
      121 gtccgcatgg tgaccccgga gtcaggatga ccgccgtcaa gatgccgcag ctcggcgaga
      181 ccgtgatcga gggaacgatc ctgaagtggc tgaaacagga gggcgaccag gtcgcgctcg
      241 acgaaccgct gttcgagatc tcgaccgaca aggtcgacac cgaggtgccg tcgtcggcgg
      301 cgggcacgct gacgaagatc ctggtaccgg agggcgagac ggtggcggtc gggaccgagc
      361 tcgcggagat ctcccaaggt ggggcgggtg gtgccgaagc tccgggagct gcgtcgcagc
      421 ccgctgcgga atactccggg ggagccgaga cctcgtcttc gaacgcgggg ggcgacgatg
      481 ctccctccgc tccggcacca ccctccgctg cggcaccggc cgcgccggca ccacccgctg
      541 cgccggctcc gacgcccgcc gcgtccccgg cagccgcttc gacggatacc gtgccttcgc
      601 agatggcggc ctcgacgcag accgggctgc cggaccgggg gccccggtcg cagatcctgt
      661 cgccgctggt tcggaagttg gcgaacgagc accgtgtgga tctctcgcag gtgagtggga
      721 ccgggacggg ggggcggatc acgaagaacg acgtgttggg cttcgtggcc acgcaggggg
      781 cggcggcacc ggcgacgagc gcgcctgcgg ccgtctcggc cgcccagccg gcgcaacccg
      841 cgcctgcggc tgcctcgacg gccgagccgg tgcaacccgc gcccccgagc gcgcccgccg
      901 ccgcgccggc ggtcgcgcag cctgcgcccg cggccatggg gggtggggag gagctcgttc
      961 cggtctcgca catccggcgg acgatcgcgc agcacatgaa ggcctcgctc gagatctcgg
     1021 cacgcgcctg gaacgcggtc gagatcaaca tggagaacgt ggcccgcgtg cgcgaacgca
     1081 cgaaggacgc gttcaaggcg cgcgagggct tcggcctcac gtacatgccg ttcgtgacgc
     1141 gggcggtcac cgaggcgctg ttggcccacc cgatggtgaa cagcgagctc cgcggcgacg
     1201 agatcgcggt caagcacttc gtcaacatgg ggatcgccgt gagctacgac gaggggctca
     1261 tcgtcccggt cctgaagggc tccgacacga tgaacctgct ggggatcgcg cgagggatca
     1321 acgatctggc cacgcgggcg cgggccaaga agctcctgcc cgacgaggtc catggcggca
     1381 cgttcacgat cacgaacccg ggaccgttcg ggtcgttgat ctcggtcccg atcatcaacc
     1441 agccccagac cggcatcctc gggttcgaca ccgtcgagaa gcgtccggtc gtgatcgacg
     1501 acacgatcgc gatccggcac atggtctaca tctcgatgtc gtgggaccac cgcgtgatcg
     1561 acggcgcgct cgcgtcgcag ttcctcgcca gggtcaaaca gaacctggag acgtgggact
     1621 tcgccgagga cctggcgctc tagcccgatg gcccgcctga tccgcccggc cggacccgtc
     1681 gactacgacg aggcgaacga gcggatgcac gcgctcgccg aacgccggct cgccggcgag
     1741 ggcgaggaca cgctgatcct gctcgaacac ccgcccgtgt acacggcggg gcggcgctgg
     1801 aagcccgacc acatcgtgtg gaccgaggac cgtatccgcg cggccggcgc cgatctccgg
     1861 ttcatcgacc gcggcgggtc gctcacgttc cacgggccgg ggcagctcgt cggctacccc
     1921 gtgttcgacc tgggggctag accggacgcg atgcggtatg tgcgcgacct cgaggaggtc
     1981 gtgatcagcg ccgcggccga cgtcggcgtc gcattgcacc gcagcgacgt ccagacgggc
     2041 gtgtggtcgg gctcgaggaa ggtgtgcgcg atcggcgtcc ggctcatgag gatgcgcgtg
     2101 tcgctgcacg gattcgcgct gaactgcacg accgacctgt cctggtacga cgcgatcgtg
     2161 ccctgcg
//
LOCUS       DATBRK010000381         7536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_937823, whole genome shotgun sequence.
ACCESSION   DATBRK010000381 DATBRK010000000
VERSION     DATBRK010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937823"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /gene="rho"
                     /locus_tag="VIH70_11225"
     CDS             <1..1618
                     /gene="rho"
                     /locus_tag="VIH70_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY8116975.1"
                     /translation="TTDKRVLEGKVAAELHQIAADLGIEGHKGLKKADLVDRIIAHTN
                     GERADGSSVAAAVSDASMASSDTTMASSDSTTSPTSGRAYVDMRDTSGNGDTRQRPQP
                     DQRDQRQRDDRREGARPPGGRPDAGQQQRDREGGPRRRPSREERRRQREERRQREIEM
                     REEELQTAPTATGLLDVLPEGYGFLRTSGYLPGQQDIYVSLSQVRRFMLRKGDTVTGK
                     VRQPKDSEKYFALLQIDSVNGMDPEEAKQRPNFDKLTPLFPNERFHLETGPTDVTGRI
                     MDMVSPIGKGQRGMVVSPPKAGKTTVLKNIASGIIQSSPETHVMVLLVDERPEEVTDW
                     QRTVQAAEVVYSTFDKPPDQHIQVTELVLERAKRLAEMGQDVAIILDSITRLARAYNL
                     AAPASGKILSGGIDSTALYPPRRFFGAARNIEEGGSVTIIASALVETGSRMDEGIFEE
                     FKGTGNMELRLDRQLAEKRLFPAINVEASSTRKEELLMPTEELALVWRLRRVLHALEP
                     GAALELLTDKIRATKSNEQFLREIAKAPAS"
     gene            1689..1991
                     /gene="rpmE"
                     /locus_tag="VIH70_11230"
     CDS             1689..1991
                     /gene="rpmE"
                     /locus_tag="VIH70_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004017962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY8116976.1"
                     /translation="MKPDIHPEYVVATVHCSCGNTFTTRSTRPELRVEICSNCHPFYT
                     GKQKLVDTGGRVERFQRRYAKQQADQAAAVKDREASRRPTVSVPPQPAEPPADEQG"
     gene            2002..2904
                     /locus_tag="VIH70_11235"
     CDS             2002..2904
                     /locus_tag="VIH70_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEY8116977.1"
                     /translation="MAQEATTPPAHFYGGQAVVEGVMMRGRDVWAVAVRRPAGDLHIE
                     SHDIDSVAKRHPVLAKPFIRGVIALGQALGIGFRALSVSARESVPENERLSSRQMGLS
                     MAIAAVIFIGLFVVLPAVAFRWVGRSVDSSIMVNALEGIFRVALFLGYLLLIGRAKDI
                     RRVFQYHGAEHKTIAAYEHEEPLVPERVDDYSTLHVRCGTNFLLLVMVITIFVFALFG
                     SPGLWWRIGSRIVAIPIIAGIAFELLRLGARFPRSRVIHALMVPGLWLQKITTKPPDR
                     AQIEVAIASFNEVLRRESELTPGT"
     gene            3007..3882
                     /locus_tag="VIH70_11240"
     CDS             3007..3882
                     /locus_tag="VIH70_11240"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEY8116978.1"
                     /translation="MSATNLDLPVLISAEQIRRREFVTIRRGYDPDQVRAYLGQLADQ
                     IELMRVLLRDAQAEAQTVRQTTARPRQDPYQQLGERVASVIREADHVAETITSEAHRD
                     AERVTREARAEADRIRTDGQAKAEEARSRAEAAVRTAREEADRTIAGLSTRRDALVDQ
                     IASMQERLIGVARDLESAMDVQFTIPEIPAMRDLLDEAAEGTPADEHATSNATAPPAE
                     PRDEAAERTGVGATAARQPTTEVAKAGDHDAGILDPFYEEPWEGADAMELDIPALDLD
                     WGEVDDEGEDHPPHA"
     gene            3934..5034
                     /gene="prfA"
                     /locus_tag="VIH70_11245"
     CDS             3934..5034
                     /gene="prfA"
                     /locus_tag="VIH70_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY8116979.1"
                     /translation="MELSDRLAELEQRYERLQGEMGSPDVAKDPDRLRRLGKDLSELG
                     EIVAPFREYRAALQQAEEATNLAKEESDPEMTAFLRGEAETAEARAAELRTHLEALLV
                     PKDPNDGKDVVVEIRAGAGGDEAALWAGTLYEMYQHQAERQRWKTEVLSASPSDLGGF
                     KEIVLEVKGRDAYSRLKHESGVHRVQRVPVTESSGRIHTSTATVAVLPAAEDVEVDIR
                     PEDLKIDVFRSAGPGGQSVNTTDSAVRITHKPTGIVVGSQEERSQLQNREKAMRYLRA
                     RLLHRAQEEAAAKEAAARRSQVGTGERAEKIRTYNFPENRVTDHRVKLTVHRLPDVLA
                     GDLDAFVDALHAAERADQLARGEDEGDVKREP"
     gene            5037..5867
                     /gene="prmC"
                     /locus_tag="VIH70_11250"
     CDS             5037..5867
                     /gene="prmC"
                     /locus_tag="VIH70_11250"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY8116980.1"
                     /translation="MRPAEVLRRASGYLDRHGVESPDATAEILLMHVLGTDRAGLYAR
                     SAGLDTREARMFGRAICQRCAGTPVQHLTGDQPFRGITLEVRPGVFVPRPETELLVEH
                     ALAEIGGRDDAVVVDAGTGTGAIALAVKDERPDATVFATDLSPEAVELARANAARLGL
                     AVTVLEGDLLDPLPAVLRGKVDVVVSNPPYVPAEDVEDLPAEVRADPVLALVGGIDVY
                     ERLGEQASRWLRGGGVLAVEIGAMLGDPVVRALSTAFSKVRVEPDLTGRDRIVLARAP
                     "
     gene            5864..6502
                     /locus_tag="VIH70_11255"
     CDS             5864..6502
                     /locus_tag="VIH70_11255"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY8116981.1"
                     /translation="MTSSLDALETAARAALDGSLIVLPTDTVYGIGTRPDDPSATARL
                     FEAKGRPRELDLPVLVPSTEDARRIAAFDERVEALAIRFWAGPLTIVLPRTGASRGWD
                     LGGDAGTIGVRMPHHPLTLALLARTGPLAVSSANRSGEPTPATCDGVEATFADAVEVY
                     LCAPEPIAGPASTVVDLAHGRPSVVRAGAVSERAVAEALRELRPPGGSEGPV"
     gene            6515..7102
                     /locus_tag="VIH70_11260"
     CDS             6515..7102
                     /locus_tag="VIH70_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116982.1"
                     /translation="MARVLVVCTGNVCRSPIAEGLLRAAFAERMAHDAPEVASAGTMG
                     WTGSGAHPSSIRAGAERGVDISGHRAREVSEEDAARADLIVAIAPEHAEAFAGDAGSR
                     TFTLKELVRLLEALPEVEDASLGRLSERIAEADRLRRDGFEGDPRDEGIPDPLGMPLD
                     AFRAVAAELDEWCSRLADGLVGQPHVRTATGSTGS"
     gene            7117..>7536
                     /gene="rpiB"
                     /locus_tag="VIH70_11265"
     CDS             7117..>7536
                     /gene="rpiB"
                     /locus_tag="VIH70_11265"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEY8116983.1"
                     /translation="MGSDHAGYPLKEALKAYLTDHGHEVLDVGTDSEEMVDYPDFCAA
                     AARAVVDGRADRGIVLGGSGQGEQLAANKVKGARAALCNDPHLAELSRRHNDANVLAM
                     GGRIVAVQLAREILQLWLETPFEGGRHERRLRQVAEIE"
BASE COUNT         1193 a         2599 c         2762 g          982 t
ORIGIN      
        1 caccaccgat aagcgtgtcc tggagggaaa ggtcgccgcg gagctgcatc agatcgcggc
       61 ggacctgggt atcgaggggc acaagggatt gaagaaggcc gacctggtcg acaggatcat
      121 cgcgcacacc aacggcgagc gtgccgatgg gtcgtccgtc gctgccgcgg tgtccgacgc
      181 ctcgatggcg tcgagcgaca cgacgatggc gtcgagcgac tcgacgacga gcccgacgag
      241 cggccgggcg tacgtcgaca tgcgggacac ctccggcaac ggcgacacgc gccagcgccc
      301 gcagccggat cagcgcgacc aacgtcaacg cgatgaccgc cgggaagggg cccggccacc
      361 gggtggccgt cccgacgccg ggcaacagca gcgcgatcgc gagggaggtc cacgccgacg
      421 tccgtcacgc gaggagcgcc gccgccagcg cgaggagcga cgccagcgag agatcgagat
      481 gcgggaggag gagcttcaga ccgcgccgac cgcgaccggg ctgctcgacg tcctgcccga
      541 gggctacggg ttcctgcgca cctccggcta cctgccgggg cagcaggaca tctacgtgtc
      601 cctctcgcag gtccgtcggt tcatgctgcg caagggggat accgtcaccg gaaaggtgcg
      661 gcagcccaag gacagcgaga agtacttcgc gctgctgcag atcgactcgg tgaacggcat
      721 ggacccggag gaggcgaagc agcggccgaa cttcgacaag ctgacgccgc tgttccccaa
      781 tgagcgcttc catctggaga ccggtccgac ggacgtgacg ggacggatca tggacatggt
      841 gtcgccgatc gggaaggggc agcgcggcat ggtggtctca ccgccgaagg cgggcaagac
      901 caccgtgctg aagaacatcg cgagcggcat catccagtcg agtccggaga cccacgtgat
      961 ggtgcttctc gtcgacgaga ggcccgagga ggtcaccgac tggcaacgga cggtgcaggc
     1021 ggccgaggtc gtgtactcga cgttcgacaa gccgccggac cagcacatcc aggtgacgga
     1081 gctcgtcctg gaacgggcca agcgcctggc cgagatgggg caggacgtcg cgatcatcct
     1141 cgactcgatc acccggctcg cgcgcgcgta caacctcgcc gcgccggcct cgggcaagat
     1201 cctgtccggc gggatcgact cgaccgccct gtacccgccc cggcggttct tcggggccgc
     1261 ccggaacatc gaggagggcg ggagcgttac catcatcgcg agcgccctcg tcgagacggg
     1321 gtcccggatg gacgagggga tcttcgagga gttcaagggc accggcaaca tggagctccg
     1381 actcgatcga cagctcgccg agaagcgcct gttccccgcg atcaacgtgg aggcgagcag
     1441 cacccggaag gaggagctgt tgatgccgac cgaggagctc gcgctcgtgt ggcgcctccg
     1501 gcgggtgctc catgcgctcg agccgggggc cgcgctcgag ctgctcaccg acaagatccg
     1561 ggccacgaag tccaacgagc agttcctacg ggagatcgcg aaggccccgg cctcgtaagc
     1621 tgggtgccgt cggacgggtc ccatcggacg ccgtccaccc accctgaaga caaggagagc
     1681 caccgaagat gaagccggac atccatcccg agtacgtggt cgcgaccgtc cactgttcct
     1741 gcgggaacac gttcacgacc cggagcaccc ggcccgagct gcgcgtggag atatgctcga
     1801 actgtcaccc gttctacacg gggaagcaga agctggttga cacgggcgga cgggtcgaac
     1861 ggttccagcg ccgctacgcg aagcagcagg ccgaccaggc cgcggcggtc aaggaccgag
     1921 aggcctcgcg caggcccacc gtctccgtcc ccccgcagcc tgccgagccc ccggccgacg
     1981 agcagggctg accctgcccg gatggcccag gaagcgacca ccccaccggc ccacttctac
     2041 gggggtcagg ccgtcgtcga aggggtgatg atgcgtggcc gcgacgtctg ggccgtcgcc
     2101 gtccgcaggc ccgccggcga tcttcacatc gagtcgcacg acatcgactc ggttgccaag
     2161 cgacacccgg tgctggcgaa gccgttcatc cgcggtgtga tcgcgctcgg gcaggccctg
     2221 gggatcgggt tccgcgcgct gtcggtctcg gcgcgggagt ccgtgcccga gaacgagcgc
     2281 ctctcgtcgc gccagatggg gctgtcgatg gcgatcgccg cggtgatctt catcgggctg
     2341 ttcgtcgtcc tgccggcggt cgcgttccga tgggtcgggc ggagcgtcga ctcgagcatc
     2401 atggtgaacg cgctcgaggg catcttccgc gtggcgctgt tcctcggcta cctgttgctg
     2461 atcggccgcg ccaaggacat ccgtcgcgtc ttccagtacc acggagccga gcacaagacc
     2521 atcgccgcgt acgagcacga ggagccgctg gtccccgagc gcgtcgacga ctactcgacg
     2581 ctgcacgtgc gatgcgggac gaacttcctc ctgctcgtga tggtgatcac gatcttcgtg
     2641 ttcgcgttgt tcggcagccc cggcctgtgg tggcgcatcg ggtcgcggat cgtcgcgatc
     2701 ccgatcatcg ccgggatcgc gttcgagctg ttgcggctcg gcgcccggtt cccgcgatcg
     2761 agggtgatcc acgcgctgat ggtgccgggg ctgtggctgc agaagatcac caccaagccc
     2821 cccgaccgtg cccagatcga ggtcgcgatc gcctcgttca acgaggtctt gcgtcgcgag
     2881 tcggagctga cgcccggcac ctgagagccc ccgagcgtag ccgcgccacg agcggccggg
     2941 atgcttgcat cgtgtgctca cgggtgtagc ctccggagcc cagcgagcgt tcgaggaggc
     3001 ttccccatgt ccgcgacgaa cctcgacctc cccgtcctga tctccgccga gcagatccgg
     3061 cgacgggagt tcgtgacgat caggcgaggg tacgaccccg accaggtccg ggcgtacctc
     3121 gggcagctcg ccgatcagat cgagctcatg cgcgtgctgc tccgcgacgc gcaggccgag
     3181 gcccagaccg tgcgccaaac gaccgcgcgt ccgcgccagg atccctacca gcagctcggg
     3241 gaacgggtcg cgagcgtgat ccgcgaggcc gaccacgtcg ccgagacgat cacgagcgag
     3301 gctcaccgcg acgcggaacg ggtgacgcgc gaggcccgcg ccgaagcgga ccggatccgg
     3361 accgacggcc aggccaaggc cgaggaggcg cgatcgcgtg cggaggccgc cgtgcggacc
     3421 gcgcgcgagg aggccgatcg gacgatcgcc gggctgtcaa ctcgccgcga cgcgctcgtg
     3481 gatcagatcg cctccatgca ggagcggctg atcggcgtcg ccagggatct ggagagcgcg
     3541 atggacgttc agttcacgat cccggagatc ccggctatga gggacctgct ggacgaggcc
     3601 gccgagggaa caccggcgga cgagcacgcc acctcgaacg cgaccgcgcc accggccgag
     3661 cccagagacg aagccgccga gcggaccggg gtgggggcaa ccgccgcacg ccaaccgacg
     3721 accgaggtgg ccaaggccgg ggaccacgac gcgggcatcc tcgacccctt ctacgaggag
     3781 ccgtgggagg gggccgacgc gatggagctc gacatccccg cgctcgatct ggactggggc
     3841 gaggtcgacg acgagggcga ggaccacccc ccgcacgcct gaggacccgc tcccgccgtc
     3901 gtagcccgcg tcacaggcct ggataatccg ggcatggaac tctccgaccg gctcgcggag
     3961 ctcgagcagc gctacgaacg tctccaaggc gagatggggt cgcccgacgt ggcgaaggac
     4021 cccgaccgcc tgcgcagact gggcaaggac ctgtccgagc tcggcgagat cgtcgctccg
     4081 ttccgggagt accgagcggc cctccagcag gccgaggagg cgaccaacct cgccaaggag
     4141 gagtcggacc cggagatgac ggcgttcctg cggggggagg ccgagaccgc ggaggcccgc
     4201 gccgccgagc tccgcaccca cctggaggcg ctcctcgtcc caaaggatcc caacgacggc
     4261 aaggacgtcg tcgtcgagat ccgcgcgggg gcgggcggcg acgaggcggc gctgtgggcc
     4321 gggaccctgt acgagatgta ccagcaccag gcggagcgtc agcgctggaa gaccgaggtg
     4381 ctctcggcct cgccgtcgga tctcggcggg ttcaaggaga tcgtgctcga ggtcaagggc
     4441 agggacgcgt actcgcggct gaagcacgag tccggcgtcc accgcgtcca gcgcgtgccc
     4501 gtgacggagt cctccgggcg catccatacg tcgacggcga ccgtggcggt gctgccggcg
     4561 gccgaggacg tcgaggtcga catccggccg gaggacctga agatcgacgt gttccgttcg
     4621 gccgggccgg ggggacagtc ggtgaacacg accgattcgg ccgtgcggat cacccacaag
     4681 ccgaccggga tcgtggtcgg gagccaggag gaacggtcgc agctgcagaa ccgcgagaag
     4741 gcgatgcggt acctgcgcgc ccgcctcctc catcgcgcgc aggaggaggc cgccgccaag
     4801 gaggccgcgg cccgacgctc gcaggtcggt accggcgagc gcgccgagaa gatccgcacg
     4861 tacaacttcc ccgagaaccg cgtgaccgat caccgcgtca agctgacggt ccaccgcctg
     4921 ccggacgtgc tggcagggga cctggacgcc ttcgtcgatg cgctgcacgc cgccgagcgc
     4981 gcggaccagc tggcccgcgg cgaggacgag ggcgacgtga aacgggaacc ctgaccgtgc
     5041 gtcccgcgga ggtcctgcga cgcgcgagcg gctacctgga tcggcacggg gtcgagagcc
     5101 ccgacgcaac ggccgagatc ctgctgatgc acgtgctcgg caccgaccgc gcggggctgt
     5161 acgcgcgttc ggccgggctc gacacgcgcg aggcccggat gttcggccgc gcgatctgtc
     5221 agcggtgcgc gggcacgccg gtgcagcacc tgaccggcga ccaaccgttc cgagggatca
     5281 cgctcgaggt ccggcccggc gtgttcgtcc ctcggcccga gaccgagctc ctcgtcgagc
     5341 acgcgctcgc cgagatcggc ggccgcgatg acgccgtagt cgtcgacgcg ggcacgggca
     5401 ccggcgcgat cgcgctcgcc gtcaaggacg agcgacccga cgcgacggtg ttcgcgacgg
     5461 acctgtcccc cgaggccgtc gagctcgccc gggcgaacgc ggcgcggctg gggctcgccg
     5521 tgaccgtcct ggagggcgac ctgctcgacc cgctgcctgc cgtcctgcgg ggaaaggtgg
     5581 acgtcgtcgt gagcaaccct ccctacgtac cggcggaaga cgtcgaggac cttccggccg
     5641 aggtgcgggc cgatccggtc ctcgcgctgg tcggcgggat cgacgtctac gagcggctgg
     5701 gcgagcaggc ctcccgatgg ttgcgcggcg gcggcgtcct ggcggtcgag atcggcgcca
     5761 tgctcggcga ccccgtggtg cgcgcgctct cgaccgcgtt ctcgaaggtc cgcgtcgagc
     5821 ccgatctcac gggccgcgac cggatcgtcc tcgcccgggc gccgtgacat cgtcgctcga
     5881 cgccctcgag acggccgcgc gggcggcgct cgacggctcg ctcatcgtcc tgccgacgga
     5941 cacggtgtac ggcatcggga ctcggcccga cgatccctcg gccaccgcgc ggctgttcga
     6001 ggcgaagggg cgcccgcgcg agctcgacct gccggtgctc gtgccctcga cggaggacgc
     6061 ccggcggatc gcggcgttcg acgagcgagt cgaggcgctc gccatccggt tctgggccgg
     6121 cccgctcacg atcgtgctgc cgaggacggg ggcgtcgcgc ggttgggacc tcggcggcga
     6181 cgccgggacg atcggggttc ggatgccgca ccacccgctg accctggctc tgctcgcgcg
     6241 caccggtccg ctcgcggtca gcagcgcgaa ccgcagcgga gagccgacgc cggcgacgtg
     6301 cgacggtgtc gaggcgacgt tcgccgacgc cgtcgaggtg tacctctgcg caccggagcc
     6361 gatcgcgggc ccggcctcga cggtggtcga cctcgcccac gggaggccgt ccgtcgtgcg
     6421 cgccggcgcg gtgtcggaac gagccgtcgc cgaggcgttg cgtgagctcc ggccgcccgg
     6481 gggctccgag ggcccggtgt agactcgcgg ccccatggca agggtcctgg tggtgtgcac
     6541 gggcaacgtg tgtcggtcgc cgatcgctga ggggctcctg cgcgccgcgt tcgcggagcg
     6601 gatggcgcac gatgcgcccg aggtcgcatc cgcggggacg atgggatgga ccggctcggg
     6661 cgcgcacccg agctcgatcc gcgcgggcgc cgaacgcggg gtcgacatct cgggtcatcg
     6721 cgcgcgcgag gtctccgagg aggacgccgc ccgggcggat ctgatcgtcg cgattgcccc
     6781 cgagcacgcc gaggcgttcg ccggcgatgc gggctcgcgc accttcacgc tcaaggagct
     6841 cgtccggctc ctcgaggcgt tgcccgaggt ggaggacgcg tcgctcggac gcctgtccga
     6901 gcgtatcgcc gaggcggacc ggctccgtcg ggacggcttc gagggcgacc cgagggacga
     6961 gggaatcccg gacccgctgg gcatgcccct ggacgcgttc cgggccgtcg ccgccgagct
     7021 cgacgagtgg tgctcgcggc tggccgacgg gctcgtaggc cagccgcatg tacgcacggc
     7081 caccgggtcg acgggatcct agacgtgcgg atcgcgatgg gatccgacca cgcggggtat
     7141 ccgctcaagg aggcgctcaa ggcctatctg accgaccacg gccacgaggt gctcgacgtg
     7201 gggaccgact ccgaggagat ggtggactac ccggacttct gcgcggccgc cgcgcgcgcg
     7261 gtcgtcgacg ggcgcgccga ccgcgggatc gtgctcggcg gttcggggca gggggagcag
     7321 ctcgcggcca acaaggtcaa gggtgcccgg gccgcgctct gcaacgaccc gcacctcgcg
     7381 gagctgtccc gccgacacaa cgacgcgaac gtgctcgcga tgggaggccg catcgtggcc
     7441 gtgcagcttg cacgggagat cctccagctc tggctcgaga cgccgttcga gggcggacgt
     7501 cacgagcgac ggctccggca ggtcgccgag atcgaa
//
LOCUS       DATBRK010000382         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1811270, whole genome shotgun sequence.
ACCESSION   DATBRK010000382 DATBRK010000000
VERSION     DATBRK010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811270"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VIH70_11270"
     CDS             <1..1156
                     /locus_tag="VIH70_11270"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEY8116984.1"
                     /translation="TSSATTGGAIREAGSMNPVVLLDEVDKLGSDYRGDPSAALLEVL
                     DPAQNHTFRDHYLEVDLDLSDVLFLATANVLDTIPGPLLDRMEMLRLDGYTEDEKVTI
                     ARDHLLARQEERNGLRSDEVEVTDEALRQVVGDYTREAGVRNLERELGKVLRKVATRV
                     ASGQAEVPVRVDAADVREYLGRPRFFFDAAERTSVPGVATGLAVTGTGGDVLFIEATR
                     LEGDGKDPLMLTGQLGDVMKESGQIALSYVRSHARELGIDASNLGGRFHVHVPAGAVP
                     KDGPSAGVTMVTAIASLLSDTPVKSTVGMTGEVTLQGRVLPIGGVKQKVLAAHRAGLK
                     EVILPKRNEGDLDDVPEQVREEIAFHPVETIDEVLSIALEREDAGAAAAA"
     gene            1252..>2951
                     /locus_tag="VIH70_11275"
     CDS             1252..>2951
                     /locus_tag="VIH70_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019667509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY8116985.1"
                     /translation="MAGAERTMRFAEGSPFPPIAEYAFLSDRQVQALVAPSGNVEWLC
                     LPRPDSPSVFGAMLDRLAGHFRLGPAEVTVPAGRRYVPGTLVLETTWQTRTGWMVIRD
                     ALLVGPWYDDTQRAVDYQRPPGDHEAEHVLLRTVRCVNGHVELLMDCEPSPDYARAGA
                     SWDYEADGYSHALARIAPDDLPLRITSDLRMGFEGKRAHANTRMRDGDERFVALSWSD
                     HEPPRSYEDAADRMWRTQEYWREWMNHGGFPDHPWRIHLQRSALTLKGLTYAPTGALM
                     AAATTSLPESPGGERNWDYRYSWIRDSTFALWGLYTLGFDSEADGFFEFVADRCQDGS
                     DLQVMYGLGGERDLTETILPNLTGYENAQPVRIGNGAYSQDQHDVWGALLDSVYLNAK
                     SRDRISERVWPIVVAQVEKAIEHWREPDRGIWEVRGEPRHFTSSKMFCWVALDRGARL
                     AGRRGEDALQEKWRAVADEIHADICANGVDERGVFTQFYGTIALDASLLLMPLLRFLP
                     KDDERVRATVLAIADELTVDGLVLRYRVEQTDDGLSGEEGTFTICSFWLVSALVEIGE
                     L"
BASE COUNT          454 a         1010 c         1064 g          423 t
ORIGIN      
        1 gacaagctcg gcaacgactg gcggggcgat ccgtgaggcc ggttcgatga acccggtggt
       61 gctgctggac gaggtcgaca agctcggctc cgactaccgg ggcgacccgt cggctgcgtt
      121 gctggaggtg ctcgacccgg ctcagaacca caccttccgc gaccactacc tggaggtcga
      181 cctcgacctg tcggacgtgc tgttcctggc cacggccaac gtgctcgaca cgatccccgg
      241 cccgctgctc gaccggatgg agatgctccg cctcgacggc tacaccgagg acgagaaggt
      301 cacgatcgcg cgcgaccacc tgctcgcgcg gcaggaggag cgcaacggcc tgcgttcgga
      361 cgaggtcgag gtcaccgacg aggcgctccg gcaggtcgtc ggcgactaca cccgggaggc
      421 aggcgtcagg aacctcgagc gtgagctcgg caaggtgctc cgcaaggtgg cgacgcgcgt
      481 cgcgagcggc caggccgagg tccccgttcg ggtcgacgct gccgacgtcc gcgagtacct
      541 cggacgcccg aggttcttct tcgacgccgc cgagcgcacg agcgtgcccg gggtcgcgac
      601 cgggctcgcg gtcaccggca ccggcggcga cgtgctgttc atcgaggcca cgcggctcga
      661 gggcgacggg aaggacccgc tgatgctcac gggccagctc ggcgacgtga tgaaggagtc
      721 gggccagatc gcgctgagct acgtgcggtc gcacgcgcgc gagctcggga tcgacgcgtc
      781 gaacctgggc ggccggttcc acgtgcacgt gcccgcgggc gcggtgccca aggacggccc
      841 gagcgccggc gtcacgatgg tgacggccat cgcctcgttg ctctcggaca cgccggtgaa
      901 gagcacggtt gggatgaccg gcgaggtgac gctgcagggc cgggtcctgc cgatcggcgg
      961 tgtcaagcag aaggtgctcg ccgcgcatcg cgccgggctc aaggaggtca tcctgcccaa
     1021 gcggaacgag ggcgacctgg acgacgtccc cgagcaggtg cgcgaggaga tcgcgttcca
     1081 tcccgtcgaa acgatcgacg aggtgctctc gatcgccctc gaacgcgagg acgcgggagc
     1141 cgcggcggcg gcctaggctg ccgagcaggc tgggggtcga gccgccggcg cgatcccagc
     1201 ccagctcggg tgatcggtaa cgcccgctcg tgggtcgcta ctatccgcac gatggccggc
     1261 gccgagcgca cgatgcggtt cgcggagggg agcccgttcc ccccgatcgc cgagtacgcg
     1321 ttcctgtccg accgccaggt gcaggcgctc gtggcgccga gcgggaacgt cgagtggctc
     1381 tgcctcccgc ggcccgacag cccctccgtg ttcggggcga tgctcgatcg cttggcgggc
     1441 catttccggc tgggtccggc cgaggtgacc gtccccgccg gccgccgcta cgtcccgggg
     1501 acgctcgtcc tcgagacgac gtggcagacg cggaccgggt ggatggtgat ccgggacgcc
     1561 ctcctcgtgg gtccctggta cgacgacacc cagcgggcgg tcgactacca acggcccccc
     1621 ggcgatcacg aggccgaaca cgtcctgctg cgcacggtcc ggtgcgtgaa cggccacgtc
     1681 gagctcctga tggactgcga gccctcgccc gactacgcgc gggcgggcgc gagctgggac
     1741 tacgaggccg atggctacag ccacgcgctc gcgcggatcg cgccggacga cctgccgctc
     1801 cggatcacca gcgatctgcg gatgggcttc gagggcaagc gcgcgcacgc gaacacgagg
     1861 atgcgcgacg gcgacgagcg gttcgtcgca ctgtcgtggt ccgatcacga accgccgcgg
     1921 tcctacgagg acgccgccga tcggatgtgg cggacgcagg agtactggcg cgagtggatg
     1981 aaccacggtg ggttccccga ccatccctgg cggatccacc tacagcgaag tgcgctcacg
     2041 ctcaagggcc tcacctacgc gcccaccggc gcgttgatgg cggcggccac cacctcgctg
     2101 cccgagtcgc cgggcggcga acggaactgg gactaccggt actcgtggat ccgggattcg
     2161 acgttcgcgc tgtggggcct gtacacgctc ggcttcgatt cggaggcgga tgggttcttc
     2221 gagttcgtcg ccgatcggtg ccaggacggc agcgacctcc aggttatgta cgggctcgga
     2281 ggcgaacgcg acctcaccga aacgatcctg ccgaacctga cggggtacga gaacgcgcag
     2341 cccgtgcgga tcggcaacgg cgcgtactcc caggaccagc acgacgtgtg gggcgcgctg
     2401 ctcgactccg tctacctgaa cgcgaagtcc cgcgaccgca tctccgaacg cgtctggccg
     2461 atcgtcgtcg cgcaggtcga gaaggcgatc gagcactggc gcgaacccga ccgcggcatc
     2521 tgggaggtcc gcggcgagcc gagacacttc acctcctcga agatgttctg ctgggtcgcg
     2581 ctcgaccgag gcgcacgcct cgcgggccgt cgtggcgagg acgcgctcca ggagaagtgg
     2641 cgcgccgtgg cggacgagat ccacgccgac atctgcgcga acggcgtcga cgagcgcggc
     2701 gtgttcacgc agttctacgg cacgatcgcg ctcgacgcct cgctgttgct catgccgctc
     2761 ctgcggttcc ttcccaagga cgacgaacgc gtccgtgcca ccgtcctcgc gatcgccgac
     2821 gagctcacgg tggacggact cgtcctgcgc taccgggtcg agcagaccga cgacgggctc
     2881 tcgggcgagg aggggacgtt cacgatctgc tcgttctggt tggtctcggc gctcgtcgag
     2941 atcggcgagc t
//
LOCUS       DATBRK010000383         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_938551, whole genome shotgun sequence.
ACCESSION   DATBRK010000383 DATBRK010000000
VERSION     DATBRK010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938551"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VIH70_11280"
     CDS             <1..253
                     /locus_tag="VIH70_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8116986.1"
                     /translation="DAIHATLDACPPELAGDIMKTGILLTGGGAMLNGLDERLRDQVL
                     MPIHVAEEPLSCVVEGAGACLDQFGAFRKLFLEDVIRDS"
     gene            617..1306
                     /locus_tag="VIH70_11285"
     CDS             617..1306
                     /locus_tag="VIH70_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEY8116987.1"
                     /translation="MSDIPAGTTPPPPPPPPASPPGPSYPVRFSVDYPDRPLDRGSTA
                     LRIFFVIPIAIVLGAVSGGTWQWSTGNQTTVVAAGAGGLLFLGPLLMILFRQKYPRWW
                     FDWNLELQRFGNRVGTYLALMEDRYPSTTEHQSVHLEYPYPDAARELNRWLPLVKWFL
                     AIPHYVVLAFLWVATVVVVIVAWFAIVFTGRYPRGVFDFVEGVIRWHNRVIAYAFVLV
                     TDVYPPFRLAP"
     gene            complement(1565..1915)
                     /locus_tag="VIH70_11290"
     CDS             complement(1565..1915)
                     /locus_tag="VIH70_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8116988.1"
                     /translation="MDPARARQLLAAERERIERDIAGLVHPDEGAAAEADEFDAGDLG
                     RELLQDELDEGRREDLQEQLAAVERAEQRLSAGTYGLSLQSGEPIPDERLEAVPTAEL
                     TVAEADEEARRLGR"
     gene            complement(2502..>2931)
                     /locus_tag="VIH70_11295"
     CDS             complement(2502..>2931)
                     /locus_tag="VIH70_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8116989.1"
                     /translation="QLAREAGAHVIGTGRAAGRQTALDFGAHEFVDLDNDTLEDVGGV
                     DLVFDVLGGDIQKRSAGLVRAGGTLVTVVGPPEARPGDGLAIDFVVESDRAQLSEIVQ
                     RVRDGRLRTNIGNVATLDDAVAALNPTERRKGKTIISVRP"
BASE COUNT          436 a          987 c          988 g          520 t
ORIGIN      
        1 cgatgccatc catgccaccc tggatgcctg tccgcccgag cttgcggggg acatcatgaa
       61 aaccgggatc ctgctcaccg ggggtggggc aatgctcaat ggtttggatg agcggctccg
      121 cgaccaggtc ctgatgccga ttcacgtggc cgaggagccg ctgtcgtgcg tggtggaggg
      181 tgccggagcc tgtctcgatc agtttggcgc gtttcgcaag ctcttcctcg aagatgtgat
      241 ccgggacagc taaagccccc atggcgccac gcagcagcat cacgaaagtg ccggcggtag
      301 tggttctgaa gagacatgga cggcccgaag cttgtcggca ggcggcgagg agaagacgcg
      361 gagtctgtgt ggagtcggca tcgacccagc tgcagcgacg cccggatgca ctggcgaccg
      421 ggtggccgcg ccgtgccgat ggatccgcgg cggcgggttg cgccgggaga gctaagtcga
      481 gcgagcccgc ccgagggctt cttccagcgt ctggatgaag gcgcctggcc tcgcaggatc
      541 ctctcggcgg cccctgggga cagctagtct tcgcggttga cggcgcaacc gaccggtttc
      601 agagagaggg gactccatga gtgacatccc agccggaaca accccgcctc cgccaccacc
      661 accgcccgcg tccccgccgg gacccagcta tccggttcgg ttctccgtgg actatccgga
      721 tcggcctctc gatcgaggga gtactgccct tcgcatcttc ttcgtcatcc cgatcgccat
      781 cgtgctcggg gccgtctccg gaggaacgtg gcagtggtcg acagggaacc agacgacggt
      841 cgttgccgcc ggagctggcg gtctgctgtt cctcgggccc ctgttgatga tcctcttccg
      901 ccagaagtac ccccggtggt ggttcgactg gaacctggag ctgcagcggt tcggcaaccg
      961 cgtcgggacc tatctggctc tgatggagga tcggtacccg tccacgacgg aacaccagtc
     1021 ggtgcacctc gagtacccct accccgatgc cgcccgcgag ctcaaccggt ggttgcccct
     1081 ggtgaagtgg ttcctcgcta tcccccatta cgtcgtgctc gcgttcctgt gggtcgcgac
     1141 cgtcgtcgtg gtcatcgtgg catggttcgc catcgtgttc actggcaggt atccacgggg
     1201 cgtgttcgac ttcgtggagg gggtgatccg gtggcacaac cgggtcatcg catatgcctt
     1261 cgtcctggtg accgatgtgt accctccgtt ccgactggct ccgtgaccag gtccttcgtg
     1321 gcgcgacccc tcagcggatc gcaggatcga gcaaccatga ggagtccgtc gcctcggacc
     1381 ggcgcgcagg aggaggaggg aggactccac gattttcccc actccctcgg tacgggacga
     1441 aagcgacgac accgtcctcc cgccggcgct gtacacgcat cgagcggagg acaacggggg
     1501 ccggcgcccg agatagagta ggtctggatc gagacagcga ttcaaacgcg cagcgtcgcc
     1561 ttcctcagcg gcccagtcgc cgagcttcct cgtcggcctc ggccacagtc agctcggccg
     1621 tcggcaccgc ctcgaggcgc tcgtcgggga tcggctctcc actctggagc gaaaggccgt
     1681 aggttccggc cgaaaggcgc tgctcggccc gctcgaccgc ggcgagttgt tcttgcagat
     1741 cctcccgccg gccctcgtca agttcgtctt gcagcagctc tcgcccgagg tcacctgcgt
     1801 cgaactcatc cgcttcggcc gcggcgccct cgtccggatg aacgagcccg gcgatatcgc
     1861 gctcgatccg ctcccgttcg gcggcgagaa gctgacgggc tcgagccgga tccacggcct
     1921 cagcgtaacg tgtcggccac cgcagaggcg ggccgcggag ggacacagcg gtcgcccggt
     1981 ggttgaggca ctctcttcga accttggcca gccaaggtag gggtcgcggg ttcgagtccc
     2041 tcgtccgctc gagcggacga gcacgacgtg cccgaacgcc ggccgcggaa gggacctcgc
     2101 tcccatcgtt tgccgtctcc gataccggtg ggaggacata tcatgggaca gaggatcttc
     2161 gtcgacggcg gccccacctg ttcgcggact tccacgagcc gtggtcgtcc gagtgacgat
     2221 cccggcgagc tgatcggcgt caactcaggc atgcgagcat cgggccgggc gcaacctcgg
     2281 cgcctcggcc ctgtggggcc tcgcgatcgg atcgtcccgc gcggtcggcg cggtcgtcgc
     2341 agggcgaacc tcgctgccgc caggagtcgc cgagttcgtc acggcgttcg gcggcggcgt
     2401 cctgctcgcg gcaatggcgc tggggtctcc cgcgccctcg ccaagcggga cgacgagacc
     2461 ccccgttacg tcgtaacggg gtctctgctg tctgggtgcc cttatggacg aacgctgatg
     2521 atcgtcttcc ccttgcgtcg ctcggtcggg ttgagggccg caacggcgtc gtcgagggtc
     2581 gcgacgttgc cgatgttcgt ccgcagtcgt ccgtcccgca cccgctggac gatctcactc
     2641 agttgggcgc gatctgactc gacaacaaag tcgatcgcca ggccgtcccc gggccgcgcc
     2701 tcgggcggcc cgacaacggt caccagtgtt cctccggctc gaaccagacc cgcggaccgc
     2761 ttctggatgt cgccgccgag gacgtcgaag accaggtcga ctccgccgac gtcttccagg
     2821 gtgtcgttgt cgaggtcgac gaactcgtgc gcgccgaagt cgagtgcggt ctgccggccg
     2881 gcggcgcgcc cggtgccgat gacgtgtgcg ccggcctctc gtgcgagctg g
//
LOCUS       DATBRK010000384         6193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1812345, whole genome shotgun sequence.
ACCESSION   DATBRK010000384 DATBRK010000000
VERSION     DATBRK010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812345"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..979)
                     /locus_tag="VIH70_11300"
     CDS             complement(5..979)
                     /locus_tag="VIH70_11300"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY8116990.1"
                     /translation="MNGHLLSIHDVSAEEIVRILDTAESFREVGTRVIKKVPALRGRT
                     VVNLFYENSTRTRISFELAAKRLSADVINFSTSGSSVSKGESLKDTALTLQAMGADAI
                     VIRHSSSGAPLTLTRWVKGSVLNAGDGTHEHPTQALLDLYTMRDRLGRLEGLRVAIVG
                     DVLHSRVARSLSFALVKMGAEITLVGPPTLIPPDAPAWGVRVSHDIGEVLPELDVCYM
                     LRVQRERQRQQYFPSVREYARLFGLSRERAGMLPDGALVMHPGPMNRGVEIDSDVADL
                     PRAVIEEQVTNGIAVRMSLLYLLLGGSGSQAADEARAVRLPEEVATGA"
     gene            complement(976..1536)
                     /gene="pyrR"
                     /locus_tag="VIH70_11305"
     CDS             complement(976..1536)
                     /gene="pyrR"
                     /locus_tag="VIH70_11305"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY8116991.1"
                     /translation="MPAARLMDEAEVRRSITRIAHEIIERNKGADGFVLVGIAARGDD
                     LARRLAGEIGRIEGRTVPVGALDITFYRDDIGIRAEAPEVHETRIDFDINGWTVVLVD
                     DVLFTGRTIRSALDALVDFGRPTAIQLAVLVDRGHRELPIRADFVGKNVPTRKDEDVR
                     VALREVDGEDAVLVQDAVTSHSGERA"
     gene            1750..1953
                     /gene="tatA"
                     /locus_tag="VIH70_11310"
     CDS             1750..1953
                     /gene="tatA"
                     /locus_tag="VIH70_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY8116992.1"
                     /translation="MNEWVIVGIVALIVFLFGAKKLPDLARSLGKSSSEFKKGMSEGA
                     SDPEAGTSDDAEKRAGGTTQTPE"
     gene            complement(1961..2377)
                     /gene="nusB"
                     /locus_tag="VIH70_11315"
     CDS             complement(1961..2377)
                     /gene="nusB"
                     /locus_tag="VIH70_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY8116993.1"
                     /translation="MSSRRQERRLAIEILFQADVTGKDGRSALAGWVESGRTVPAFTR
                     ELVEGVSDHLAELDRTIGAHSEGWTVDRMASVDRTVLRAAAYELAFDRAVPVGVAIDE
                     AVRAAKDLSTDDSGRFVNGILGKIASELDPRAEGPS"
     gene            complement(2382..2939)
                     /gene="efp"
                     /locus_tag="VIH70_11320"
     CDS             complement(2382..2939)
                     /gene="efp"
                     /locus_tag="VIH70_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY8116994.1"
                     /translation="MSTNDLKNGMTLELDGTLFQVVEFQHVKPGKGGAFVRTKLRNVR
                     TGSVVERTFNAGVKVGLAIVERKDMQYLYRDGESLTFMDMSSYEQIPVPAGIVGTGAS
                     WLSEGATAQVAMHQGTPIGVELPASVTLAITRTQPAVKGDTKTNAMKPAVLETGAEIQ
                     VPMFVEEGERVKVDTRSGAYMERVK"
     gene            complement(2942..4024)
                     /locus_tag="VIH70_11325"
     CDS             complement(2942..4024)
                     /locus_tag="VIH70_11325"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY8116995.1"
                     /translation="MDHGLRRSHLGARLTELGVEGLVVTRLPNVRYLTGFTGSNAQVV
                     VAVDGSAVLLTDGRYGAQSSHEVPDLPRTVYRQGYPQPLVDACRTLGIDRAGFERHAL
                     TYEGWEQLTDRAEGIELVPVGPEVEQLRRTKDPEETALIDRAQGCADRAFETVVLGGG
                     LREGVTERAFALVLETAMVEAGAEDRAFDSIVAFGDHAAEPHHHLTDRGLARGDMVKL
                     DFGARVDGYHSDITRTVAFGEPDPRLQEIRDVVAAAQRAGIEAVRPGISTIEVDAAAR
                     SVIVDAGHGDAFPHGLGHGVGLEIHEAPSLRWDGGEELVEGMVVTIEPGVYIPGLGGV
                     RIEDMVEVTAHGRREIPRATKELMVL"
     gene            complement(4009..4443)
                     /locus_tag="VIH70_11330"
     CDS             complement(4009..4443)
                     /locus_tag="VIH70_11330"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY8116996.1"
                     /translation="MRVLFLFGPNLGALGRREPERYGTQTLEEIMDEVAERGDRLGHE
                     LTWRQTDHEGELIGWLNGAAAEGFQAVVANPGALSHTSYALRDAVEGCGLAVIEVHMT
                     NIYAREEFRRHSVISAACRATIAGAGAGGYHVALEALPWITG"
     gene            complement(4446..5549)
                     /gene="aroB"
                     /locus_tag="VIH70_11335"
     CDS             complement(4446..5549)
                     /gene="aroB"
                     /locus_tag="VIH70_11335"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY8116997.1"
                     /translation="MERVSVPMPGRAYEVCIGTGVLAHADELVPALAGAERAFVIADA
                     PVAERYLAALSDGLAARGLGVVHLGVPEGEEAKSLQVMNALERQLATQVAHRDDPIVA
                     LGGGAVGDLAGFVAATYMRGVPFVQVPTTLTAQVDAAIGGKTGVNIPEGKNLVGAFHQ
                     PIAVLADVGTLATLPDRAFLSGLAEVAKYALTLDLELLEMLEHDPSPLLEREPALLEE
                     VVTRCVRAKAAVVAVDERDEGRRLVLNYGHTLGHALERLDAFAGRTHGEAISPGMVFA
                     ARLSEALGRGPSGLAARHVRLLASLGLETDGGLPPPEEVLSAIRMDKKYRGGARFVLL
                     EDVGRPFVEEAVPEALLRTTLDRSGSGAQAMGG"
     gene            complement(5537..6037)
                     /locus_tag="VIH70_11340"
     CDS             complement(5537..6037)
                     /locus_tag="VIH70_11340"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY8116998.1"
                     /translation="MIVYLVGMPGSGKSTVGPELARRLGVPFVELDERIEHAAGSSVR
                     EIFERDGEARFRELEAAALVEAASRDPSVVSCGGGVVLEPANRVSLRATGEVVFLSVP
                     LEVLAERVRPGADRPLIRSEGDLERVFRDREPLYREFAAHVVDASGPVEDVAAAIEKE
                     LLRWSA"
     gene            complement(6069..>6193)
                     /locus_tag="VIH70_11345"
     CDS             complement(6069..>6193)
                     /locus_tag="VIH70_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY8116999.1"
                     /translation="AVVGEATVAFVLAQAVLEKFGGDALPETRRNLDAFVAGLR"
BASE COUNT          883 a         2311 c         2105 g          894 t
ORIGIN      
        1 cggttcaggc acccgtggcc acctcctccg gcagccggac ggcgcgggcc tcgtcggccg
       61 cctgggagcc ggaccctccg aggaggaggt acaggagcga catccgaacg gcgatcccgt
      121 tcgtcacctg ctcctcgatg accgcccgag gcagatcggc gacgtccgag tcgatctcga
      181 caccacggtt catcgggccc gggtgcatca cgagcgcgcc gtccggtagc atcccggccc
      241 gctcgcgcga caggccgaac aggcgcgcgt actcgcggac actggggaag tactgctggc
      301 gctgacgctc gcgttgcacg cggagcatgt agcagacatc gagctcgggc agcacctccc
      361 cgatgtcgtg gctcacccgc acgccccacg cgggggcgtc cggcgggatc agcgtcggcg
      421 gaccgacgag cgtgatctcg gcgcccatct tgacgagcgc gaacgagagc gaccgcgcca
      481 cgcgcgagtg cagcacgtct ccgacgatcg ccacgcggag gccctcgagc cgcccgagcc
      541 ggtcgcgcat cgtgtacagg tcgagcaggg cctgggtcgg gtgctcgtgg gtgccgtcgc
      601 ccgcgttcag cacgctgccc ttcacccagc gggtgagcgt gagcggcgcg ccgctggaag
      661 aatggcggat cacgatcgcg tcggcgccca tggcctgcag cgtgagcgcg gtgtccttca
      721 ggctctcgcc cttggacacg ctcgagccgc tcgtcgagaa gttgatcacg tcggcggaga
      781 gccgcttggc cgcgagctcg aagctgatcc gggtccgcgt cgagttctca tagaacaggt
      841 tcacgacggt ccggccacgg agcgccggga ccttcttgat gaccctcgtg cccacctcgc
      901 ggaacgactc ggccgtgtcg aggatccgca cgatctcctc ggcgctcacg tcgtggatcg
      961 acagcaggtg tccgttcatg ctcgctcccc cgagtgactg gtcacggcgt cctggacgag
     1021 gacggcgtcc tcgccgtcga cctcccgcag ggccacccgc acgtcctcgt ccttgcgggt
     1081 cggcacgttc ttgccgacga agtcggcgcg gatcggcagc tcgcggtgtc cccggtcgac
     1141 gagcaccgcg agctggatcg cggtcgggcg cccgaagtcc acgagcgcgt cgagcgccga
     1201 gcggatcgtc cgccccgtga acaggacgtc gtcgaccagg acgacggtcc acccgttgat
     1261 gtcgaagtcg atccgggtct cgtgcacctc gggcgcctcg gctcggatac cgatgtcgtc
     1321 ccggtagaag gtgatgtcga gcgcgccgac ggggacggtc cgcccctcga tccggccgat
     1381 ctcccccgcc aggcgccgcg cgaggtcgtc gccgcgggcg gcgatcccca ccaggacgaa
     1441 cccgtcggcg cccttgttgc gctcgatgat ctcgtgcgcg atgcgcgtga tggaccggcg
     1501 cacctcggcc tcgtccatca gcctcgcggc cggcatcagg ccgccccggg ggccgtcaag
     1561 aaggaacgcc cggccgcgcg tgcggcaggg cgtcgtggtc gagcacacac cgtcttcggc
     1621 tcccttcccg gcctctcggg accggactta aagggcggaa gttcgcggcg aggataccac
     1681 ggccccggtg cggacctccg agcgccgcgg gcgtacactg gcacccgaca ccgaagggcg
     1741 ggagtccgca tgaacgagtg ggtcatcgtc ggcatcgtgg cactgatcgt cttcctcttc
     1801 ggagcaaaga agctccccga tctcgcccgg tcgctgggca agtcctcgag cgagttcaag
     1861 aaggggatgt ccgaaggcgc ctcggatccg gaggccggca cctcggacga cgcggagaag
     1921 cgcgccgggg gcacgacgca gacacccgag tagcctcccc tcagctcggc ccctcggccc
     1981 gcgggtcgag ctcgctcgcg atcttgccga ggatcccgtt cacgaaccgc cccgagtcgt
     2041 ccgtggacag gtccttggcc gcccgcaccg cctcgtcgat cgccacgccg accggcaccg
     2101 ctcgatcgaa cgccagctcg taggcggccg cccgaaggac cgtacggtcg accgatgcca
     2161 tccggtcgac ggtccacccc tccgagtgtg ccccgatcgt ccggtcgagc tccgcaaggt
     2221 gatcggacac gccctcgacg agctcgcggg tgaacgccgg caccgtccgt ccggactcga
     2281 cccagccggc cagggccgac cgtccgtcct tccccgtcac gtccgcctgg aacaggatct
     2341 cgatcgcgag gcgccgctcc tgcctgcgcg agctcaagca gtcacttgac ccgttccatg
     2401 taggcgcccg acctcgtgtc gaccttcacg cgctccccct cctcgacgaa catcggcacc
     2461 tggatctcgg cgcccgtctc gaggacggcg ggcttcatgg cgttggtctt ggtgtcgccc
     2521 ttcacggcgg gttgggtcct cgtgatcgcc agcgtgacgg aggccgggag ctcgaccccg
     2581 atcggggtgc cctggtgcat ggccacctgc gcggtcgctc cctccgagag ccagctcgcg
     2641 ccggtcccga cgatcccggc cgggaccggg atctgctcgt acgacgacat gtccatgaac
     2701 gtcagggact cgccgtcccg atagaggtac tgcatgtcct tgcgttcgac gatcgcgagg
     2761 ccaaccttga cgccggcgtt gaaggtgcgc tcgacaaccg agccggtcct cacgttgcgg
     2821 agcttcgtgc ggacgaacgc tcctcccttg cccggcttga cgtgctggaa ctcgaccacc
     2881 tggaacagcg tgccgtcgag ctcgagcgtc atgccgttct tcaggtcgtt ggtggacacg
     2941 ctcacagcac catcagctcc ttggtggctc gcgggatctc gcggcgtccg tgggcggtca
     3001 cctcgaccat gtcctcgatc cggacgcctc cgagcccggg aatgtacacg ccgggctcga
     3061 tcgtgacgac catcccctcg accagttcct cccccccgtc ccagcgcagc gacggcgcct
     3121 cgtggatctc gaggccgacc ccgtgaccga gcccgtgcgg gaacgcatca ccgtgtcccg
     3181 cgtcgacgat caccgagcgc gccgcggcgt ccacctcgat cgtggagatc ccggggcgca
     3241 ccgcctcgat ccccgcccgc tgcgccgcgg cgaccacgtc tcggatctcc tgcaagcgcg
     3301 ggtcgggctc tccgaacgcc acggtccgcg tgatgtcgga gtggtacccg tccacacgcg
     3361 cgccgaagtc gagcttgacc atgtcgccgc gggcgagccc ccggtcggtg aggtggtggt
     3421 ggggttcggc cgcgtgatcg ccgaacgcga cgatcgagtc gaacgcgcga tcctcggcgc
     3481 cggcctcgac catcgcggtc tcgagcacca gcgcgaacgc gcgctcggtc acgccctcgc
     3541 gcaggccgcc gcccaggacc accgtttcga acgcacggtc cgcgcagccc tgtgcgcggt
     3601 cgatcagcgc ggtctcctcg ggatccttgg ttcggcgaag ctgttcgacc tcggggccga
     3661 ccggcacgag ctcgatgccc tcggcccggt ccgtgagctg ctcccatcct tcgtacgtga
     3721 gcgcgtggcg ttcgaacccc gcccgatcga tcccgagggt ccgacaggcg tcgaccagcg
     3781 gttgcgggta cccctgccgg tagacggtcc gcggcaggtc gggcacctcg tggctcgact
     3841 gcgcaccgta tcggccgtcg gtcagcaaca cggccgagcc gtccaccgcg accacgacct
     3901 gtgcgttgga cccggtgaac cccgtgaggt agcgaacgtt gggcagccgc gtgacgacca
     3961 gcccctcgac cccgagctcg gtgaggcgtg ccccgaggtg cgaacgtctc agcccgtgat
     4021 ccacgggagc gcctccagcg cgacgtggta gccgcccgcg cctgcaccgg cgatcgtcgc
     4081 gcggcacgcc gccgagatca cggagtgccg gcggaactcc tcgcgcgcgt agatgttcgt
     4141 catgtggacc tcgatcaccg ccagcccgca gccctcgacc gcgtctcgca gggcgtacga
     4201 ggtgtgcgag agcgcgccgg ggttggcgac gacggcctgg aacccctcgg ccgcggcgcc
     4261 gttcagccac ccgatcagct cgccctcgtg atcggtctga cgccacgtga gctcgtggcc
     4321 gagcctgtcg cctcgttcgg ccacctcgtc catgatctcc tcgagcgtct gcgtgccgta
     4381 ccgttcgggc tcccggcgtc cgagtgcccc gaggttcggg ccgaacagga acagcacccg
     4441 catcctcaac cccccatcgc ctgcgcgccc gagccggacc ggtcgagcgt ggtacgcagc
     4501 agcgcttccg ggaccgcttc ttccacgaac gggcggccga cgtcctccaa cagtacgaag
     4561 cgcgccccgc cgcggtactt cttgtccatc cggatggccg agaggacctc ctcggggggc
     4621 gggagcccgc cgtcggtctc gagtcccagg gacgcgagca accggacgtg ccgcgccgcg
     4681 aggcccgacg ggccacggcc gagcgcctcg gacagccggg cggcgaacac catccccggg
     4741 gagatcgcct cgccgtgggt ccggccggcg aacgcgtcga ggcgctcgag cgcatggccg
     4801 agcgtgtggc cgtagttcag gactaggcgg cggccttcgt cgcgttcgtc caccgcgacc
     4861 acggccgcct tggcgcgcac gcaccgggtc accacctcct ccaggagcgc cggttcccgc
     4921 tcgagcaggg gtgagggatc gtgttcgagc atctcgagca gctcgagatc gagggtgagc
     4981 gcgtacttcg ccacctccgc gagcccgctc aggaacgccc gatcgggcag ggtggcgagg
     5041 gtccccacat cggccagcac cgcgatgggt tggtggaagg cgcccacgag gttcttgccc
     5101 tccgggatgt tgacgccggt cttgccgccg atcgccgcgt ccacctgggc ggtgagcgtc
     5161 gtgggcacct gcacgaacgg gacgccgcgc atgtacgtgg ccgcgacgaa cccggcgagg
     5221 tcccccaccg ctccgccccc gagcgcgacg atcgggtcgt cccggtgcgc cacctgggtg
     5281 gcgagctggc gttcgagcgc gttcatgacc tgcagcgact tggcctcctc gccctcgggc
     5341 acgccgaggt gcaccacgcc cagaccgcgc gcggcgagac cgtccgacag ggcggcgagg
     5401 tagcgctcgg cgacgggagc gtcggcgatc acgaacgcgc gctcggcgcc cgcgagcgcc
     5461 gggacgagct cgtcggcgtg cgcgagcacg cccgtcccga tgcagacctc gtacgcccgc
     5521 cccggcatcg gaacgctcac gcgctccatc gcaggagctc cttctcgatc gccgccgcca
     5581 cgtcttccac gggtccgctc gcgtcgacga cgtgggcggc gaactcgcgg tacagcggct
     5641 ctcggtcacg gaacacgcgc tcgaggtcgc cttcggagcg gatcagcggg cggtcggccc
     5701 cgggacggac ccgctccgcg agcacctcga gtgggaccga gaggaacacc acttcgccgg
     5761 tcgcccgcag cgagacgcgg ttggccggtt cgagcacgac cccgccgccg cacgagacga
     5821 ccgacgggtc gcgcgacgca gcctcgacga gggccgccgc ctcgagctcg cggaagcggg
     5881 cctcgccgtc gcgttcgaag atctcgcgga ccgaggagcc cgcggcatgc tcgatgcgct
     5941 cgtccagctc gacgaagggg acgccgagcc gccgcgcgag ctccgggccc acggtcgact
     6001 tgcccgaccc cggcatcccg acgaggtaga cgatcacgat ccgcccgccg cggcggcgcg
     6061 tgtggcgttc accgcagccc ggccacgaac gcgtcgaggt tgcgccgcgt ctcggggaga
     6121 gcgtcgccgc cgaacttctc gaggaccgcc tgcgccagca cgaacgcgac cgtcgcctcg
     6181 ccgaccaccg cgc
//
LOCUS       DATBRK010000385         7069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1377424, whole genome shotgun sequence.
ACCESSION   DATBRK010000385 DATBRK010000000
VERSION     DATBRK010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377424"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /gene="dnaK"
                     /locus_tag="VIH70_11350"
     CDS             <1..1383
                     /gene="dnaK"
                     /locus_tag="VIH70_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY8117000.1"
                     /translation="DKQHDQTILVFDLGGGTFDVSLLEIGEGVFEVKSTHGDTQLGGD
                     DWDQRVIDWMVKEFKNNYGIDLGVDRMALQRLKEAAEKAKIELSQVSEATINLPFVSA
                     GADGPLHLEMKLTRSEFERMTEDLVRRTREPFELAVKDWGKNVEAIDHIILVGGSTRM
                     PMIQELVRELTGGKEPHKGVNPDEVVAVGAAIQAGVLKGDVKDILLLDVTPLTLGVET
                     KGGIFTRLIERNTTIPTRKSEIFTTADDNQTNVEIHVLQGEAETVYSPGVRSLGKFML
                     TGLPPAPRGMPQIEVAFDIDANGIVNVSAKDMATGKEQAMTITGGTALSKEDIDRMVQ
                     DAEKFAAEDHTRREAAEARNQADQLTYQVDRSVEEWGDKVPEPEREELKKLNEELKES
                     LKGEDVEKIKAGTEAVMQTFQRVGQAMYQQTPQGQQTAAAAAGGAPGSEPEGENDVVE
                     GEIVDEGGER"
     gene            1490..2128
                     /locus_tag="VIH70_11355"
     CDS             1490..2128
                     /locus_tag="VIH70_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY8117001.1"
                     /translation="MTERDDRDEPRPKVRVVDKRRYRDGGSAEAAPAPAEEPSGAGST
                     GATTGSAPRPQRSAGSAGGPDELAQARTEAAEYRDHLQRLQAEFENYRKRMTRSQERL
                     VDAEVQRLVQGLLEVLDEFDLALIAAERQPDFEAFRKGVELVYAKLAETLRAEGLERI
                     DAQGKAFDPNEHEALMQTGDGEGDPRVAEIFRQGYKLRGTVIRPASVRVERT"
     gene            2142..3281
                     /gene="dnaJ"
                     /locus_tag="VIH70_11360"
     CDS             2142..3281
                     /gene="dnaJ"
                     /locus_tag="VIH70_11360"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY8117002.1"
                     /translation="MNGDVRREWFDKDYYKVLGVAKNASAAEIKKAYRKLAQQHHPDA
                     NPGDTQAEDRFKEISAAYDVLGDEDKRTQYDQVRDMASSGFGSGGFPGGGGGFEGGFP
                     GGVRFEDLGDLGDLFGGLFGGGRRGGGARPARGADLETTVTVSFDDAMTGTTRPVRIS
                     GPAPCETCHGSGAAPGTSPTICPRCGGSGAVAVNQGLFQMSQPCPVCHGSGRVVQTPC
                     PTCGGTGSRRRTRSFQVKVPAGVQDGARIRLGGRGEPGPAGAQPGDLYVQVRVQPHAF
                     FGRSGHDLTLELPVTYAEAALGADVRVPTLNGPVTMKVPAGTPNGKTFRLKGKGAPKR
                     GGHGDLLVTTRIEVPRKLSRPEKDLLKQLQQTEKESPRRRLGVEA"
     gene            3295..3732
                     /locus_tag="VIH70_11365"
     CDS             3295..3732
                     /locus_tag="VIH70_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010842656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8117003.1"
                     /translation="MSETRGRGSERGTDGAEERAVYIISVAAELSGAHPQTLRMYERR
                     GLLAPKRTSGNTRRYSERDIARIQMIQELTQRDGVSLAGVKLFIEMREQLAEMRRRTE
                     ELERELLEQMRRRRSGGEIVPLRSLRSWPWEGENTDDAEDAAT"
     gene            3729..4295
                     /locus_tag="VIH70_11370"
     CDS             3729..4295
                     /locus_tag="VIH70_11370"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8117004.1"
                     /translation="MIELSGDRVRLRGFRPDEVDLALDRMQSIPASELDAEERRERRE
                     RIEHSGERGESEIVLAIEADGRVVGDVQGRCPRFAMPRGVWELGIELWDEADRGRGFG
                     TQTVALLSAYLFEREDAIRVQATTDVDNAPMRGVLERLGFAFEGVLRGFMPDPGGPPR
                     DYAMYGLTEAAWNDEKRDGRRDRWTPTS"
     gene            4277..6889
                     /gene="clpB"
                     /locus_tag="VIH70_11375"
     CDS             4277..6889
                     /gene="clpB"
                     /locus_tag="VIH70_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY8117005.1"
                     /translation="MDANKLTMKSQAALEAARQQAVARSHQAIEPEHLLFALLSDPEG
                     IVFPVLHGMGVAPKPLRDQADASLDRLPKVYAPRQAGDVEARLGPSLGTALDAAFREA
                     EAMGDDYVSTEHLLLGLLEGTSNAARILRDAGVERDALLSALAQVRGRRRVTDPNPED
                     KVQVLERYGRDLTELARKGSLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTA
                     IVEGLAQRIVSGDVPESLKDKRVVALDLGAMVAGSKYRGEFEERLKAVLTEIAESEGQ
                     VVTFIDELHTIVGAGAAEGAMDAGNMLKPMLARGELRAIGATTLDEYREHIEKDPALE
                     RRFQPVLVGEPSVEDAIAILRGLKERYEVHHGVRIQDPALVAAAVLSDRYVTGRFLPD
                     KAIDLIDEAASRLRMEIDSVPVEIDEVERRIMQLEIERAALRKESDQASRERLDRLEQ
                     ELADLQERSAAMRGHWQQEKERIDRIRALKGELESTRGEFERAERDADLERAAELRFG
                     RLVELGKELERENLALEEIQRDRKMLNEEVTEEDVAQVVSMWTGIPVARLMEGEMAKL
                     LHLEEALHHRVVGQDEAVSAVANAIRRSRAGLSDPTRPIGSFLFLGPTGVGKTELARA
                     LAEYLFDDERAMVRIDMSEYQERHTVSRLVGAPPGYIGFEEGGQLTEAVRRRPYSVLL
                     LDEIEKAHTDVFNVLLQLLEDGRLTDGHGRTVDFRNAVVIMTSNLGGAIFRETEISAD
                     KRREAVLEDVQGFFRPEFVNRIDEIVVFEPLTPEQIGAIVEIQIRLLAERLAGRSLGL
                     ELTEAARDLIAARGYDPAFGARPLKRLIQREIQDPLAVKLLSGEIGEGDTVLVDADGE
                     SLTFAGTSAAICGP"
BASE COUNT         1180 a         2327 c         2640 g          922 t
ORIGIN      
        1 gacaagcagc acgaccagac gatcctcgtg ttcgacctgg ggggcgggac gttcgacgtc
       61 tcgctgctcg agatcggcga gggcgtcttc gaggtcaagt ccacccacgg cgacacccag
      121 ctgggcggcg acgactggga ccagcgagtg atcgactgga tggtcaagga gttcaagaac
      181 aactacggga tcgacctcgg ggtcgaccgc atggcgctcc agcgcctcaa ggaggccgcc
      241 gagaaggcga agatcgagct ctcgcaggtg agcgaggcca cgatcaacct gccgttcgtc
      301 tcggccggcg cggacggccc gctccacctg gagatgaagc tcacgcgcag cgagttcgag
      361 cgcatgaccg aggatctggt ccggcgcacc cgcgagccct tcgagctcgc ggtgaaggac
      421 tggggcaaga acgtggaggc gatcgaccac atcatcctgg tcggcgggtc tacgcggatg
      481 ccgatgatcc aggagctcgt cagggagctc accggtggca aggagccgca caagggcgtg
      541 aacccggacg aggtcgtggc cgtcggcgcc gcgatccagg ccggcgtcct caagggcgac
      601 gtgaaggaca tcctgctcct ggacgtgacg ccgcttaccc tcggcgtcga gaccaagggt
      661 ggcatcttca cccgcctcat cgagcgcaac acgacgatcc cgacccgcaa gtcggagatc
      721 ttcacgactg ccgacgacaa ccagaccaac gtcgagatcc acgtcctgca gggcgaggcg
      781 gagaccgtct actccccggg ggtgcgctcg ctcggcaagt tcatgctcac ggggctcccg
      841 cccgcaccgc gcgggatgcc gcagatcgag gtcgccttcg acatcgacgc caacggcatt
      901 gtcaacgtgt cagccaagga catggccacc ggcaaggagc aggcgatgac gatcaccggc
      961 ggcaccgcgc tgtccaagga ggacatcgac cggatggtcc aggacgcgga gaagttcgcc
     1021 gccgaggacc acacccgccg agaggccgcc gaggcgcgca accaggccga tcagctcacg
     1081 taccaggtcg accgttcggt cgaggagtgg ggcgacaagg tgcccgagcc cgagcgggag
     1141 gagctcaaga agctcaacga ggagctgaag gagtccctca agggcgagga cgtcgagaag
     1201 atcaaggccg gtacagaggc cgtgatgcag acgttccagc gggtcggcca ggcgatgtac
     1261 cagcagacgc cgcagggaca gcagacggcg gccgccgcgg ccggcggcgc gccggggtct
     1321 gagcccgagg gcgagaacga cgtggtggaa ggcgagatcg tggacgaagg aggagagcga
     1381 tgagggatcg cgtgctcacg gtcgaggtgc ccaggatcga gaaggccaag ccgaagggga
     1441 tccagatcag ggccggccga tcgaaggccg atcggaccaa ggcggacgcg tgaccgagcg
     1501 cgacgaccgc gacgagccgc gaccgaaggt ccgtgtggtg gacaagcggc ggtaccgcga
     1561 cggcggctcg gccgaggccg cgcccgcgcc ggccgaggag ccgagcgggg cgggttcgac
     1621 cggcgcgacg acggggagcg cccctcgtcc ccagcggtcg gcggggtcgg ccggcggtcc
     1681 ggacgagctc gcgcaggccc ggacggaggc cgccgagtac cgcgatcacc tccagcggct
     1741 gcaggcggag ttcgagaact accgcaagcg catgacccgc tcccaggagc ggctcgttga
     1801 cgccgaggtc cagcggctcg tgcaggggct cttggaggtg ttggacgagt tcgacctcgc
     1861 gttgatcgcg gccgagcggc agccggattt cgaggccttc cgcaagggcg tcgagctcgt
     1921 ctacgcgaag ctcgccgaga cgctgcgtgc cgagggcctc gagcggatcg acgcccaggg
     1981 caaggccttc gatcccaacg agcacgaggc cctgatgcag accggcgacg gcgagggcga
     2041 tccgcgcgtc gcggagatct tccggcaggg gtacaagctg cgcggcaccg tgatccgtcc
     2101 ggcgagcgtc cgcgtcgagc ggacctaggt cggaggcggc cgtgaacggc gacgtccgac
     2161 gcgagtggtt cgacaaggac tactacaagg tgctcggcgt cgcgaagaat gcgagcgccg
     2221 ccgagatcaa gaaggcctac cgcaagctcg cgcagcagca ccaccccgac gccaaccccg
     2281 gggacacgca ggccgaggac cggttcaagg agatctcggc ggcgtacgac gtgctgggcg
     2341 acgaggacaa gcgcacgcag tacgaccagg ttcgcgacat ggcttcgtcc gggttcggct
     2401 cgggcgggtt ccccggcggt ggcggcgggt tcgagggcgg gttccccggc ggggtccggt
     2461 tcgaggacct cggcgatctc ggggacctgt tcggcgggct gttcgggggc ggccgtcgcg
     2521 gcggcggcgc ccgtcccgct cgcggggcgg atctggagac gaccgtgacg gtctcgttcg
     2581 acgacgcgat gaccggaaca acgaggccgg ttcggatctc cggcccggcg ccgtgcgaga
     2641 cctgtcacgg aagcggcgcc gcgccgggca cgagcccgac gatctgtccg cgatgcggcg
     2701 ggtcgggcgc cgtcgcggtc aaccaggggt tgttccagat gagccagccc tgtcccgtct
     2761 gtcacggctc ggggcgcgtc gtgcagacgc cgtgcccgac gtgtggcggg accgggagcc
     2821 gccggcggac gcggagcttc caggtcaagg tcccggccgg cgttcaagac ggcgcccgga
     2881 tcaggcttgg cggccgaggc gagccaggtc ccgccggcgc gcagcccggg gacctgtacg
     2941 tgcaggtccg cgtgcagccc cacgcgttct tcgggcgttc ggggcacgac ctgacgctcg
     3001 agctgcccgt cacctacgcg gaggcggccc tgggcgccga cgttcgcgtc ccgacgctga
     3061 acggtccggt gaccatgaag gtgcccgcgg gcacccccaa cgggaagacc ttccgcctga
     3121 agggcaaggg cgcgcccaag cgagggggcc acggggacct gctcgtcacc acccgcatcg
     3181 aggtccctcg caagctgtcc agacccgaga aggatctgct gaagcagctg caacagaccg
     3241 agaaggagtc gccgcgccgg cgtctggggg tggaagcatg aggaggcact cgtcgtgagc
     3301 gagacacgcg gtcgcgggtc ggaacggggg accgatggtg cggaggagcg agccgtctac
     3361 atcatcagcg tggcggccga gctctcgggc gcgcatccgc agacgcttag gatgtacgaa
     3421 cgccggggcc tcctcgcgcc caagcgcacg tcgggcaaca cgcgtcgcta ctccgaacgc
     3481 gacatcgcgc gcatccagat gatccaggag ctcacgcagc gcgacggcgt gagcctggcg
     3541 ggcgtcaagc tgttcatcga gatgcgcgag cagctcgcgg agatgcgccg gcgcaccgag
     3601 gagctcgagc gcgagctgct cgagcagatg cgccgccgcc gctccggcgg cgagatcgtc
     3661 ccgctgcgaa gccttcggtc gtggccgtgg gagggagaga acaccgacga cgcggaggac
     3721 gccgccacgt gatcgagctc agcggcgacc gagtgcgcct gcgcggtttc cgtcccgacg
     3781 aggtcgacct cgcgctggac cggatgcagt cgatccccgc gtccgagctg gacgccgagg
     3841 agcggcgcga gcggcgcgag cgcatcgagc attcgggcga gcgcggtgag tcggagatcg
     3901 tgctggcgat cgaggcggac ggccgcgtgg tcggcgacgt ccagggacga tgcccccgct
     3961 tcgccatgcc ccgcggggtg tgggagctcg ggatcgagct gtgggacgag gccgatcggg
     4021 gccgagggtt cgggacgcag acggtggcgc tgctgtccgc ctacctgttc gagcgcgagg
     4081 acgcgatccg cgtgcaggcg accaccgacg tggacaacgc gccgatgcgc ggggtgctcg
     4141 agcggctggg cttcgcgttc gagggcgtgc tccgcggttt catgccggat cccggggggc
     4201 ccccgcgcga ctacgcgatg tatggcctga ccgaggccgc ctggaacgac gagaagaggg
     4261 acggccggag ggaccgatgg acgccaacaa gctgacgatg aagtcgcagg ccgcgctcga
     4321 ggcggccaga cagcaggccg tggcccgcag ccaccaggcg atcgagcccg agcacctgct
     4381 gttcgcgctc ctgtcggacc ccgagggcat cgtgttcccg gtcctgcacg gcatgggcgt
     4441 ggcacccaag ccgctgcgcg accaggccga cgcgtcgctc gaccggctgc cgaaggtcta
     4501 cgcgccgagg caagcgggag acgtggaggc ccggctcggc ccctcgctgg gaacggcgct
     4561 cgacgcggcg ttccgcgagg ccgaggcgat gggcgacgac tacgtctcga ccgagcacct
     4621 gctcctgggg ctgctcgagg ggacgtcgaa cgcggcgcgg atcctccgcg acgcgggggt
     4681 cgagcgcgac gcgctgctgt ccgcgctcgc gcaggtgcgc ggccggcgcc gcgtcaccga
     4741 cccgaacccc gaggacaagg tccaggtgct cgagcgctac ggccgcgacc tgacggagct
     4801 ggcccgcaag gggtcgctcg acccggtgat cggccgcgac gacgagatcc ggcgggtgat
     4861 ccaggtgctc tcccgccgga cgaagaacaa cccggtcctg atcggcgaac ccggcgtggg
     4921 caagaccgcc atcgtggagg gcctggcgca gcggatcgtc tcgggcgacg tgcccgagtc
     4981 cctcaaggac aagcgcgtgg tcgcgctcga tctcggcgcg atggtggccg gctcgaagta
     5041 ccggggcgag ttcgaggagc ggctgaaggc cgtcctgacc gagatcgccg agtccgaggg
     5101 gcaggtcgtc acgttcatcg acgagctgca cacgatcgtg ggcgccggcg cggccgaggg
     5161 cgcgatggac gccggcaaca tgctgaagcc gatgctcgcg cgcggcgagc ttcgcgcgat
     5221 cggcgccacc acactggacg agtaccgcga gcacatcgag aaggacccgg cgctcgaacg
     5281 ccggttccag cccgtgctgg tcggcgaacc gagcgtcgag gacgcgatcg ccatcctgcg
     5341 ggggctgaag gagcgctacg aggtccacca cggcgtgcgg atccaggacc cggcgctggt
     5401 cgccgccgcc gtcctgtcgg accggtacgt gaccggccgg ttcctgcccg acaaggcgat
     5461 cgatctgatc gacgaggcgg cgagccggct gcggatggag atcgattcgg tcccggtcga
     5521 gatcgacgag gtggagcgcc gcatcatgca gctggagatc gagcgcgccg ccctgcgcaa
     5581 ggagagcgac caggcgtccc gtgagcggct cgaccggctc gagcaggagc tcgccgacct
     5641 gcaggaacgc tcggccgcga tgcgcggcca ctggcagcag gagaaggagc ggatcgaccg
     5701 gatccgggcg ctcaagggcg agctcgagtc cacgcgcggc gagttcgagc gcgccgagcg
     5761 cgacgccgat ctcgagcggg ccgccgagct ccgtttcggc cggctcgtcg agctcgggaa
     5821 ggagctcgag cgcgagaacc tggccctgga ggagatccag cgcgatcgga agatgctgaa
     5881 cgaggaggtc accgaggaag acgtcgcgca ggtggtctcg atgtggaccg gcatcccggt
     5941 cgcccgcctg atggagggcg agatggccaa gctcctgcac ctggaggagg cgctccatca
     6001 ccgcgtcgtc gggcaggacg aggccgtctc cgccgtcgcg aacgcgatcc ggcgctcccg
     6061 agccgggctc tcggatccga cgcggccgat cggctcgttc ctgttcctgg gcccgaccgg
     6121 cgtgggtaag accgagcttg ctcgcgcgct cgccgagtac ctgttcgacg acgagcgcgc
     6181 catggtccgc atcgacatga gcgagtacca ggagcgtcac accgtgtcgc gcctggtcgg
     6241 tgcgccgccc ggctatatcg ggttcgagga aggtggccag ctcaccgagg ccgtccgtcg
     6301 gcgtccctat tccgtcctgc tgctcgacga gatcgagaag gcgcacaccg acgtgttcaa
     6361 cgtcctgttg cagctgctcg aggacgggcg tctcaccgac gggcacgggc gaacggtcga
     6421 cttccgcaat gcggtggtga tcatgacctc caacctcggt ggcgccatct tccgggagac
     6481 cgagatctcg gccgacaagc gtcgcgaggc ggtcctggag gacgtgcagg gcttcttccg
     6541 tccggagttc gtgaacagga tcgacgagat cgtcgtgttc gagccgctca cgcctgaaca
     6601 gatcggtgcg atcgtggaga tccagatccg tctgctcgcg gagcgcctcg ccgggcggtc
     6661 gctgggcctc gagctcaccg aggcggcccg cgacctcatc gcggcccgtg gctacgatcc
     6721 cgcgttcggg gctcggccgc tgaagcggct catccagcga gagatccagg acccgctcgc
     6781 cgtgaagctg ctctccggcg agatcggcga gggagacacg gtgctcgtgg acgccgatgg
     6841 ggagtccctg acgttcgccg gtacctccgc cgccatctgc ggcccctagc gggtgagccg
     6901 gcggcgagcg cctcgggtcg tcgccgtacg agcgaggggc taggcttccc gtcgacctct
     6961 gcagaaggga acgaccgtgg gttggaccat cgcgatcatc ggcgggatcg tcctgctgat
     7021 cctgatcttc ggcgtcgtct cgtacaaccg attcgtctcc cagcgtcag
//
LOCUS       DATBRK010000386         4619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2677186, whole genome shotgun sequence.
ACCESSION   DATBRK010000386 DATBRK010000000
VERSION     DATBRK010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677186"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VIH70_11380"
     CDS             <1..344
                     /locus_tag="VIH70_11380"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006460772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEY8117006.1"
                     /translation="EDAESRGVEVFIAAAGMAAALPGVVAAHTTRPVLGVPMESKLAG
                     GLDSLLSMVQMPGGIPVGVLAVGKAGAVNAAILAAQILGRSHPELQEAVWKQREAKAA
                     EILANPDPSEA"
     gene            complement(362..2152)
                     /locus_tag="VIH70_11385"
     CDS             complement(362..2152)
                     /locus_tag="VIH70_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8117007.1"
                     /translation="MARVGSLTYHLSSTPRGAPIENPAAEPAPALSEIRRDLVVSARA
                     LVASFRNPDLARAQLALVAFGICEWASFIALMVFAFEQGGAAAIGIISLVQLIPAAVI
                     APLGSFLGDRFPREKVFRLAEASMSAACVLAAVAVLLDAPSILIYAAACTVGWLLTLV
                     RPTHGALLPWLARDPTELTTAYTAAGLIESLCVLLGPLLATAFFALGTALGVAGPGLV
                     YAALAVLLGIGALLLSGVHSQAPPPDPQGRTSVVWSEMSAGFRYVWSDPRPRALVGTL
                     GLWTFVLGFIDTLIVVLALDVLGIGQTGVGALNAALGIGGVIGAAVAVVAGSRERLFP
                     AYRAGSFLYGVPVAAIGAVPPLAPVLLGLAGSGGVLYDVSGRTMLQRLIPDQKLTRSL
                     GVLESVYMGSEGLGAFAAAALVTLAGPRWTFAIAGVIVPSVCFVLRRRLRAVDVGARV
                     PADDLALLRRTSLFEPLPAAAVERLARNSVPVVVDAGTTIIREGDVGDRVYVIAAGEA
                     DVSANGSHVAMLGPGEHVGEIALLRDVPRTATVVATTDVRLLSLERDVFLRTMTGHEP
                     AHAAAHSAAEDRLRELAVPEPPGTIAPPEG"
     gene            2215..3123
                     /locus_tag="VIH70_11390"
     CDS             2215..3123
                     /locus_tag="VIH70_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY8117008.1"
                     /translation="MATAVWTGTLSFGLVTLPVRLYPATQPKDVRFHLYDPEGHRIRY
                     QRVVETGAEPPSDWNELRPSDSARDEIATPMREERPATRGPVETPVAWEDVVRGVETD
                     EGELVTVPREELERIRPERSRFIDIEDFVELEQIDPVYLDKSYYAIPMTPEATKPYVL
                     FHHAMREGGRIGIGRFVLRTKPHLVAIRPMEKVLAVETMFFGDEVRDPADLAPDLDGV
                     EVDERELDLAVTLIETLKTEWDPDAYADTYRKELLGMLAERTPTTRPAEEPAPAGGSA
                     VEELMAALKESVAAAQRDRTRKKATG"
     gene            complement(3131..3838)
                     /locus_tag="VIH70_11395"
     CDS             complement(3131..3838)
                     /locus_tag="VIH70_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8117009.1"
                     /translation="MTLKIRTDDQISDVDGGWFRARWHYSFDTYLDPEYTSFGTMRVF
                     NDDRLVPGAVWPMHPHRDIEGLTYVVEGLFRHEDDAGGAPGPLPAGSVQRMTLGRGAY
                     HSEQNASETEPMRFIQIWIMPAERGLPPGVEQKVFTTEDRTDTLLKAISGDDGDAVLV
                     HQDAHVFVSRLSAGRSVEHPLAPGRGVYLYVIEGDVGVNGERMETGSAAQITDEERAE
                     VAASSASELILVDVALG"
BASE COUNT          759 a         1630 c         1604 g          626 t
ORIGIN      
        1 tcgaggacgc cgagtcccgc ggcgtggagg tcttcatcgc ggccgcgggc atggcggcgg
       61 ccctgcccgg cgtcgtggcc gcccacacca cgcgacccgt gctcggcgtc ccgatggagt
      121 cgaagctcgc gggcgggctc gactcgctgc tctcgatggt gcagatgccg ggcgggatcc
      181 cggtcggcgt gctcgccgtc ggcaaggcgg gcgcggtgaa cgcggcgatc ctggccgccc
      241 agatcctcgg tcgatcgcat ccggagctcc aggaggccgt ttggaagcag cgcgaggcca
      301 aggccgccga gatcctggcc aaccccgacc cctccgaagc ctagaccgat ggcttcctga
      361 ctcagccttc cggtggcgca atggtgcccg gcggctcggg aacggcgagc tcgcgcagcc
      421 ggtcctcggc ggccgaatgg gccgcggcgt gcgccggctc gtgcccggtc atcgtgcgca
      481 ggaacacgtc gcgttcgagc gagagcagcc gaacgtcggt cgtcgcgacg acggtggcgg
      541 tgcgggggac atctcggagc agggcgatct cgcccacgtg ctcgcccggc cccagcatgg
      601 cgacatggct cccgttcgcg gacacgtcgg cctcgcccgc cgcgatcacg tagacgcggt
      661 cgccgacatc gccctcgcgg atgatcgtgg tcccggcatc gacgacgacc gggacgctgt
      721 tgcgtgcgag gcgttcgacc gccgccgcgg gaagcggttc gaacagcgag gtccgtcgga
      781 gcagcgcgag gtcgtcggca gggacccgag cgccgacgtc gaccgcccgc aggcgccgac
      841 ggaggacgaa gcacaccgat ggcacgatca cgccggcgat cgcgaacgtc catcgcggtc
      901 ccgccagcgt gacgagcgcc gcagccgcga acgcgccgag cccttcgctc cccatgtaga
      961 ccgactcgag cacgcccagg gatcgcgtga gcttctggtc cgggatcagg cgttgcagca
     1021 tggtccgacc cgacacgtcg tacaggacgc cgcccgagcc ggccaggccg agcaggaccg
     1081 gtgcgagcgg gggaacggcg ccgatcgccg ccaccggaac gccgtagagg aacgaccccg
     1141 ctcggtaggc cgggaacagg cgttcccggc tccccgcgac caccgcgacc gcggcgccga
     1201 tcaccccgcc gatgccgagc gcggcgttga gggcgccgac gcccgtctgg ccgatcccga
     1261 gcacgtcgag cgccagcaca acgatcaggg tgtcgatgaa cccgaggacg aaggtccaca
     1321 gcccgagggt cccgaccagc gccctcggcc ggggatcgga ccacacgtac cggaaccccg
     1381 cgctcatctc gctccacacc accgacgtcc ggccctgcgg atccggaggt ggcgcctggc
     1441 tgtgtacccc ggacagcagc agggcgccga tgccgaggag cacggcgagc gccgcgtaca
     1501 cgagccccgg ccccgcgacg ccgagggcgg tgccgagcgc gaagaaggcc gttgcgagca
     1561 gcggaccgag caggacgcac aggctctcga tcaggccggc ggccgtgtac gcggtcgtga
     1621 gctcggtcgg gtcgcgcgcc agccagggca gcagggcgcc atgcgtcggc cgcaccagcg
     1681 taaggagcca gccgacggtg caggcggcgg cgtagatcag gatcgagggc gcatcgagca
     1741 ggaccgcaac ggcggccagg acgcatgcgg cgctcatcga cgcttcggcg agcctgaaga
     1801 ccttctctcg cgggaaccgg tcaccgagga acgagccgag cggcgcgatc accgccgcgg
     1861 ggatcagctg gaccagggag atgatcccga tcgccgccgc gccaccttgc tcgaacgcga
     1921 acaccatcag ggcgatgaag ctcgcccact cgcagatgcc gaacgcgacg agcgcgagct
     1981 gcgcgcgagc gaggtcgggg ttgcggaacg aggccaccag ggctcgagcc gagacgacca
     2041 ggtcgcgcct gatctcggac agagcagggg ccggctcggc ggcgggattt tcgatcggtg
     2101 ctcctcgtgg cgtgctcgat aggtggtacg tgagcgaccc gacccgggcc accgggacac
     2161 ggtacgcccg ggaccggcct tccgcctcac ggcggctctc gatacccttg acccatggcc
     2221 accgccgtgt ggacgggtac cctgtcgttc gggctggtga cgctccccgt gcggctgtat
     2281 cccgcgacgc agcccaagga cgttcgcttc cacctgtacg atcccgaggg ccatcgcatc
     2341 cgctaccagc gcgtggtcga gacgggggcg gaacctccgt cggactggaa cgagctccgc
     2401 ccctccgaca gcgcccggga cgagatcgcc acccccatgc gggaggagcg cccggccaca
     2461 agggggcccg tcgagacgcc ggtcgcgtgg gaagacgtcg tgcgcggcgt cgagaccgac
     2521 gagggcgaac tcgtgacggt gccgcgggag gagctcgagc ggatccgtcc cgagcggagc
     2581 cgattcatcg acatcgagga tttcgtcgag ctcgagcaga tcgacccggt ctacctcgac
     2641 aagagctact acgcgatccc gatgacgccc gaggcgacca agccctacgt tctgttccat
     2701 cacgcgatgc gggagggggg gcggatcggg atcgggaggt tcgtgctgcg aaccaagccg
     2761 cacctcgtgg cgatccgccc gatggagaag gtgctggccg tcgagacgat gttcttcggc
     2821 gacgaggtcc gagacccggc cgatctcgcc cccgacctgg acggcgtcga ggtcgacgag
     2881 cgggagctgg acctcgcggt cacgctcatc gagaccctca agacggagtg ggatcccgac
     2941 gcctacgccg atacctaccg gaaggagctt ctcggcatgc tcgccgaacg cacgccgacg
     3001 acgcgcccag ccgaagagcc ggcaccggcc ggggggtccg cggtggagga gctgatggcc
     3061 gccctcaagg aaagcgtcgc cgcggcccaa cgcgaccgga cccgcaagaa ggccaccggt
     3121 taagccgggc ctacccgagc gccacgtcga ccaagatcag ctcgctcgcc gagcttgccg
     3181 cgacctccgc gcgctcctcg tccgtgatct gggctgccga ccccgtctcc atccgctcgc
     3241 cgttcacccc gacgtctccc tcgatcacgt agaggtacac cccgcgtccg ggcgcgagcg
     3301 ggtgctcgac cgagcgaccc gcgctcaggc gggagacgaa cacgtgcgcg tcctggtgca
     3361 cgagcacggc gtcgccgtcg tcaccgctga tcgccttcag gagggtgtcg gtccggtcct
     3421 cggtcgtgaa gaccttctgc tcgacgccgg gtggcagacc acgttcggcg ggcatgatcc
     3481 agatctggat gaaccgcatc ggttcggtct cggaggcgtt ctgctccgag tggtacgccc
     3541 cgcgcccgag ggtcatgcgc tggaccgagc ccgccggaag cggtcccggt gccccgcccg
     3601 cgtcgtcctc gtggcggaac agcccttcga ccacgtaggt cagcccctcg atgtcgcggt
     3661 gagggtgcat cggccacacg gcgcccggca cgagccgatc gtcgttgaac acccgcatcg
     3721 tgccgaacga cgtgtactcg gggtcgaggt acgtgtcgaa cgagtagtgc cagcgggcac
     3781 ggaaccaccc gccgtccacg tcggagatct ggtcatccgt gcggatcttc agcgtcatcg
     3841 gccggctccc gacgccaacc tacccggatt cacatccgcg cgcgtggcct agagcggggg
     3901 cccttgagcc ggggaatgag cagtcggatc cacgggcctc caccgctgcg ccacaccgtg
     3961 aagccctcgg gcgttggggt gcctgcccac accatcgata cctcgggcgg caaagagacg
     4021 gacatctctg gcggcggcac atcgagcatg aggccaagct gagcgtcgaa ctgtcgccac
     4081 tcacccacgc gaatagatgc cgcaccccag ctaccttggc gagcttcgtg cgctcaactt
     4141 cgaaccgagg tccgtccagg gattcctgga tcaactcgtg cgtatgcccc gcccaggcca
     4201 ccccgccgac gctcggcggc atgacgaaca cctttccagg tccctcccgg ctcgactgcg
     4261 cgaagtcgac cgggagctga tcgatagctc ggtcatatac cccaggtggg cgccatttcg
     4321 gcctttgcca gaattcgccc tctgcaggct cgagttcggc taacgccaca acgagatcgc
     4381 gcttcatctc gctgaggcgc ggaccctgta cggccagcgt cagcgagagc cacataccat
     4441 cggcgggtga gttggggggc gtcgatgatg ccttgctccc caatcgccgc gtagagggcg
     4501 cggagttgtg gatcggcgag ctgtgtcacc tcaagccctc caacaacgtc gcggtctcgg
     4561 atcgaagtcg tgggtggcgt caggtgctcc gggaacatca cggtgatgtg tctggacac
//
LOCUS       DATBRK010000387         6244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1521213, whole genome shotgun sequence.
ACCESSION   DATBRK010000387 DATBRK010000000
VERSION     DATBRK010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521213"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VIH70_11400"
     CDS             <1..1132
                     /locus_tag="VIH70_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8117010.1"
                     /translation="FGYSSYDFLSDGRIVCTYRQRGVHHLALLDPATRELLDLDLPYE
                     TFEPAVSVSGQRLAFIAGGPATPTRVVSLDFSTRAVEVLRESDVVEFDPRYLSVPAPI
                     EFPTTDGQAAFAHYYPPTNPMAVAPAGERPPLLVNVHGGPTSEADPELHLEIQFFTSR
                     GFAYAEVNYRGSTGYGRGFRESLYGRWGVVDVDDAVAAARHLVEGGLADPDRLIISGG
                     SAGGWTTLCALTFRDAFATGTSYFGVSDLEPFAETTHKFEARYLDVLVGPWPEAAELW
                     AQRSPVRHAELLSSPLLILQGDEDEVVPPAQAEVIVRALQGRGLPYAYLVFEGEQHGF
                     RKMESISRGLSAELTFYGKILGFEPADRLPPLEIVNLPSGGE"
     gene            complement(1310..1480)
                     /locus_tag="VIH70_11405"
     CDS             complement(1310..1480)
                     /locus_tag="VIH70_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117011.1"
                     /translation="MLSGEMARYQIGDRVREAEAERLARSTRRSRAAESRSVTRRIGR
                     AALAAAAWPIKH"
     gene            1571..2314
                     /locus_tag="VIH70_11410"
     CDS             1571..2314
                     /locus_tag="VIH70_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117012.1"
                     /translation="MTSVTLRRGAAGSKMRSVIRRLAFGAPPLLAALVAVVVSAGPAS
                     AQSTSPSPASGPSVEGEVSGGLAVGSTLTIRVDGTMPGGWEALHLLEATITSGNRELD
                     RLRFDIEDNKLTVGDQDLIVGTGATATSEYLRVSGANVIVTTGGANLSFEVDAHVVKT
                     IPEGARFVLGVVDDFGESAEVTRSLAAPEGGGFTWGTVVALIAAALFAGGFVGNILAN
                     KRRPPPRASVYDAVQRRVETDRAAKGRST"
     gene            2311..4464
                     /locus_tag="VIH70_11415"
     CDS             2311..4464
                     /locus_tag="VIH70_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8117013.1"
                     /translation="MSRQDVYRLLAEDIASNGAGEHADVWETITRLVDPTELRPKLAD
                     DIEIRVFDQRWGEGYVMIANPRDLLHFRLAPSDLGLLELMDGTRTVKEIVVERFEGSG
                     EMELSTVIDLVRSLYRGNFLEQRHADVPEMVTRAMDPVTDRRRKARQFGKTLSIEWEG
                     AHRLVRWLYDHGLKWVFKRAVLVALIAIAFVGLGAFVDILRHEQFVIAGRSLAIGFVV
                     LIVLDYVMVFCHELGHALVIVHNGRRIRSAGFQIYFGSPAFFVESSDGLMMDRHQQVI
                     ESFAGPFAQLVLAGIASLIARSYPEWILSETMYKFAVLNYLVVIMNLIPLLELDGYYI
                     LADLIEIPDLRERSLVFMRRDLFHKLRTREHFSKRDAGLALYGVLGIAFTIFSLYTAY
                     YYWQTVFGDLVSRLWAEGPVTRLLLLVLAAFIAGPLLRGLIKLLRAIGREIRAVWRRL
                     RFRLETGWRVEASELIDALAMFEDIPVDALNEIAGRVRLRGLGDGQAVFRQGDQPGAF
                     YVVRKGVLEVVEEDTESGGERRLRDLGRGESFGELGLVTAAPRAATVRAVGAAEVFEV
                     DKGVFDHLLADKLHLPDFEPTLQQVAELRGLSCFANLSTAQLWELLEAGAWVSFVPGD
                     TIMRQGDVGDSFYAISSGQVDIVKDERLVNTIGRGSYFGEVALLMEVPRTATVMARTP
                     VRAFRLDRGGFDRLVGDAFRRGTLISHAVTEELTH"
     gene            4471..4686
                     /locus_tag="VIH70_11420"
     CDS             4471..4686
                     /locus_tag="VIH70_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117014.1"
                     /translation="MDCWHCKRSAVGVCRFCGRGICEDHVETHPYILELFRTGDVPKV
                     LIVEDALFCGACTPRPDPLDLPELDSM"
     gene            4828..4956
                     /locus_tag="VIH70_11425"
     CDS             4828..4956
                     /locus_tag="VIH70_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117015.1"
                     /translation="MADKKISPKAPAAKDKKESGKAEAATRVSRVKRVKRVKRVKK"
     gene            5064..5771
                     /locus_tag="VIH70_11430"
     CDS             5064..5771
                     /locus_tag="VIH70_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8117016.1"
                     /translation="MRTTELSKALLEGMARTPLFEGASDQTISRLAAQGVTRRFRRGT
                     YLFLQGDDAPDVYFLVSGRVEISSLSSNGHRQLHTTLDVPQFFGELGVLGEQPRSAAA
                     LALEDTEAWRVSGPVLLDFLIEEPTAARALLRALARQVLSQEAFVEDLLYLDLKGRVA
                     KRLLQLVSPSLDELPSDGAVVPSIVTHADLASLCGGSRENVTRILSDFQRRGIVERDG
                     KRFVLVKISGLAKLAGL"
     gene            5803..6096
                     /locus_tag="VIH70_11435"
     CDS             5803..6096
                     /locus_tag="VIH70_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117017.1"
                     /translation="MSEATAAHDWLEGLAFVLGLAFLALAVLCVWGIVTAIANIGDAQ
                     APPGEPVTVGFLEATRGFALVLFALFGFTAFGVGWFLAGDTVKKWFARIKARS"
BASE COUNT          894 a         2107 c         2193 g         1050 t
ORIGIN      
        1 cttcggctac tcgtcgtacg acttcctctc cgacgggagg atcgtgtgca cgtaccgaca
       61 gcgaggcgtc catcacctcg cgctgctcga tccggcgacg cgggagctcc tggacctcga
      121 cctgccgtac gagaccttcg agccggcggt cagcgtctcg gggcagcggc tcgccttcat
      181 cgccggcggt ccggccacgc cgacgcgggt cgtctcgctc gacttctcga cgcgcgccgt
      241 cgaggtcctg cgcgagagcg acgtcgtcga gttcgatccc cggtacctgt ccgtcccggc
      301 gccgatcgag ttcccgacca ccgacgggca ggccgcgttc gcgcactatt atccgccgac
      361 gaacccgatg gccgtcgctc ccgcggggga acgtccgccg ttgctcgtca acgtccacgg
      421 tggaccgacg tcggaggcgg acccggagtt gcacctcgag atccagttct tcaccagccg
      481 tgggttcgcc tacgccgagg tgaactaccg ggggagcacc ggctacgggc ggggcttccg
      541 cgagagcctg tacggccgtt ggggcgtcgt cgacgtcgac gacgcggtgg ccgccgcccg
      601 gcacctggtc gagggcggtc tcgccgaccc cgatcgcctc atcatctccg gtgggagcgc
      661 cgggggatgg acgacgctat gtgcgctcac cttccgggac gcgttcgcga cggggacgag
      721 ctacttcggc gtgtccgatc tcgagccgtt cgcagagacc acccacaagt tcgaggcgag
      781 atacctcgac gtcctggtcg gtccttggcc cgaggcggcc gagttgtggg cgcaacgctc
      841 ccccgttcgc cacgccgagc tcctgtcaag cccgctgctg atcctgcagg gtgacgagga
      901 cgaggtcgtg ccgcccgccc aggcggaggt catcgtccgg gccctccagg gcaggggtct
      961 gccctacgcg tacctcgtgt tcgagggcga gcaacacggc ttccgcaaga tggagagcat
     1021 ctcgcggggt ctgtcggccg agctcacctt ctacgggaag atcctgggat tcgaacccgc
     1081 cgatcgactc cccccgctcg agatcgtgaa cctgccgtcc ggtggggaat gaccgtttgc
     1141 ggcgatcggg gtacgtcgca ccgtccgcgc gtacgagagg gggcgcgggg ggcccggtcg
     1201 aagacccgac cccccgcttt cgagaggggg cgcgtgcctc tcgtagcgaa tggaacgtat
     1261 cgccggcccg tgaaggccgc gcggcgcgcg gatgtcgggg ccgtgaacgc tagtgcttga
     1321 tcggccaggc ggcggccgcc agggcggcac gaccgatgcg gcgggtgacg ctccgcgact
     1381 ccgcggccct gctcctgcgg gtcgaccggg ccagccgctc ggcctcggcc tctcgaacgc
     1441 ggtcgccgat ctggtaccgg gccatctctc cgctcagcat ctccgcacct cctcgacgtt
     1501 gcggtccatc tctcgcgaac ggtatgcgcc gtccgcggct ccgagcggtg acgccacgca
     1561 cctcgcgggg gtgacctccg tcacgttgcg ccggggggcc gccgggtcga agatgcgctc
     1621 cgtgatccgt cggcttgcgt tcggagcgcc gccgctgctc gccgccctcg tcgcggtggt
     1681 cgtttccgcg gggcccgcct cggcgcagtc cacctcgccg agcccggcga gcggtccgag
     1741 cgtcgagggg gaggtctcgg gcggcctcgc ggtgggctcg acgctcacga tccgcgtcga
     1801 cgggaccatg ccgggtggct gggaggcgct ccacctcctc gaggcgacga tcacgtccgg
     1861 gaaccgggag ctcgatcggc tgcggttcga catcgaggac aacaagctga cggtcggcga
     1921 ccaggatctg atcgtcggca ccggcgcgac ggcgaccagc gagtacctgc gggtgagcgg
     1981 cgccaacgtc atcgtgacca ccggaggcgc caacctctcc ttcgaggtcg acgcccacgt
     2041 cgtgaagacg atcccggagg gcgcacggtt cgtcctgggc gtcgtcgacg acttcggcga
     2101 gtcggccgag gtcaccaggt cgctggcggc ccccgagggc ggggggttca cctgggggac
     2161 cgtggtcgcg ctcatcgcgg ccgcgctgtt cgccggtggg ttcgtgggca acatcctcgc
     2221 gaacaagcgg cgcccgccgc cgcgcgcctc ggtctacgac gcggtccaac gcagggtcga
     2281 gaccgatcgc gccgccaagg gccgctcgac gtgagccggc aggacgtcta ccgcctcctc
     2341 gccgaggaca tcgcttcgaa cggcgcggga gagcacgccg acgtctggga gacgatcacg
     2401 cgccttgtcg atcccaccga gctccgtccc aagctcgcgg acgacatcga gatccgggta
     2461 ttcgatcagc ggtggggcga ggggtacgtc atgatcgcga accctcgcga tctcctccac
     2521 ttccggctcg ctccctcgga tctcggcctg ctcgagctga tggacgggac ccgcaccgtc
     2581 aaggagatcg tggtcgagcg gttcgagggg tccggcgaga tggagctgtc caccgtgatc
     2641 gacctcgtcc gctcgctgta ccggggcaac ttcctcgaac agcggcacgc ggacgtcccc
     2701 gaaatggtga cgcgggcgat ggacccggtc accgaccggc gccgcaaggc gcggcagttc
     2761 ggcaagacgc tctcgatcga gtgggagggc gcccatcggc tggtgcggtg gctctacgac
     2821 cacggcctga agtgggtctt caagcgggcg gtcctcgttg cgctgatcgc catcgcgttc
     2881 gtcgggctgg gtgcgttcgt cgacatcctg cggcacgagc agttcgtgat cgccggtcgt
     2941 tccctcgcga tcggcttcgt cgtgctgatc gtcctggact acgtgatggt gttctgtcac
     3001 gagctcgggc acgctctcgt catcgtgcac aacggccgac ggatccgcag cgcgggattc
     3061 cagatctact tcgggtcccc ggcgttcttc gtcgagagct ccgacgggct gatgatggat
     3121 cggcaccagc aggtcatcga gtcgttcgca ggcccgttcg cgcagctcgt cctggccggc
     3181 atcgcgtcgc tcatcgcacg gtcgtacccg gagtggatcc tgtcggagac gatgtacaag
     3241 ttcgcggtcc tgaactacct ggtcgtcatc atgaatctga tcccgctgct cgagctcgac
     3301 ggctactaca tcctggccga cctgatcgag atccccgatc tgcgcgaacg ctcgctcgtg
     3361 ttcatgcgtc gcgacctgtt ccacaagctt cggacgcgcg agcacttctc caagcgggac
     3421 gcgggcctcg ccctctacgg ggtgctcggc atcgcgttca cgatcttctc cctctacacg
     3481 gcctactact actggcagac cgtgttcggg gacctcgtga gccggctgtg ggccgagggg
     3541 ccggtgacca ggctgctcct gctcgtcctc gccgcgttca tcgccgggcc actgctccgg
     3601 ggtctgatca agctcctgcg ggcgatcggc agggagatcc gcgcggtgtg gcgccggctc
     3661 cggttccggc tggagaccgg gtggcgggtc gaggcctccg agttgatcga cgcgcttgcg
     3721 atgttcgagg acatcccggt cgacgccttg aacgagatcg ccgggcgcgt ccggcttcgt
     3781 gggctgggcg acggacaggc ggtgttccgc cagggggacc aacccggcgc cttctacgtc
     3841 gtgcgcaagg gggtcctgga ggtggtcgag gaagacaccg agagtggcgg tgagcgccgg
     3901 ttgagagacc tcggccgggg cgagtccttc ggagagctgg ggctggtgac cgcggcgccg
     3961 cgagccgcca ccgtccgcgc cgtcggggcc gccgaggtgt tcgaggtcga caagggcgtg
     4021 ttcgaccatc tgctcgccga caagttgcat ctgcccgact tcgagccgac cctgcaacag
     4081 gtcgccgagt tgcgaggcct gagctgcttc gccaacctgt ccaccgcgca gctctgggag
     4141 ctcctggagg caggggcgtg ggtgagcttc gttcccggcg acacgatcat gcgccagggc
     4201 gacgtcgggg attcgttcta tgcgatatcg tccggccagg tcgacatcgt caaggacgag
     4261 agactggtga atacgatcgg ccgcggatca tatttcggcg aggtcgccct tctgatggag
     4321 gtgccccgaa cggcaaccgt gatggcgcgg actcccgtcc gtgcgttccg cctcgatcgg
     4381 ggagggttcg atcgcctggt gggggacgcg ttccgtcgtg ggaccctgat ctctcatgcg
     4441 gtcaccgaag agctgaccca ctgagccggt atggactgct ggcactgcaa gcggagcgcg
     4501 gtcggggtct gccgtttctg cggccggggg atctgcgagg accacgtcga gacgcatccg
     4561 tacatcctcg agctcttccg gaccggcgac gtcccgaagg tgttgatcgt cgaggacgcg
     4621 cttttctgcg gggcctgcac cccccgcccc gacccccttg acctgcccga actcgactcc
     4681 atgtgacgca cgtcaccggt ttcgatgtga cggaggtcac ctggcttcga gtgacgcccg
     4741 acacacgcgc gacgtgacgc cgcgacgatg atgcggggag acaacgaaag ccctagaggg
     4801 ccagagcgga ggggggaggt gacgatcgtg gcggacaaga agatctcacc gaaggcgccg
     4861 gctgccaagg acaagaagga gtcgggcaag gcggaggctg ccacccgtgt ttcccgggtc
     4921 aagcgggtca agcgggtcaa gcgggtcaag aagtaggaat ccagaacttg gcatgaggcc
     4981 gggtcgcgat cggcgccccg gcctcatcgc gttcggggac aacccccgcg accgggagca
     5041 cagatgtgac tacgcttgca gccgtgcgga cgacggagct cagtaaggcg ctgctggagg
     5101 ggatggctcg gactcccctg ttcgagggcg cgtcggacca gacgatctcg cgactcgcgg
     5161 ctcagggcgt gacgcggagg ttccgtcgcg ggacgtatct gttcctccag ggtgacgacg
     5221 cgccggacgt ctacttcctc gtcagcggcc gcgtcgagat cagctcactg tcgtcgaacg
     5281 gtcaccgtca gctccacacg acgctggacg tcccccagtt cttcggggag ctcggggtgc
     5341 ttggtgagca gccgcgctcg gcggccgccc tcgcgctcga ggacacggag gcatggcggg
     5401 tgagcggccc ggtactcctg gacttcctga tcgaggagcc gacggccgct cgtgccttgc
     5461 tccgggccct cgcccggcag gtgctgtcgc aggaggcgtt cgtcgaagac ctgctgtacc
     5521 tggacctgaa ggggcgggtc gccaagcgcc tcctgcaact ggtctcccca tcgctggacg
     5581 agctcccatc cgacggcgcc gtcgtgccgt cgatcgtgac gcacgccgac ctggcgagcc
     5641 tgtgcggcgg gagccgagag aacgtcacga ggatcctgtc cgacttccag cgccgcggca
     5701 tcgtcgagcg tgacggcaag cggttcgtgc tcgtgaagat ctcggggctc gccaagctcg
     5761 ccggcctgtg aaccgatgac ctaccgcacc ttccgccgcc ggatgagcga ggcgaccgcc
     5821 gcccacgact ggctcgaggg gctcgccttc gtgctgggcc tggcgttcct ggccctcgcc
     5881 gtcctgtgcg tgtgggggat cgtcaccgcg atcgcgaaca tcggcgatgc gcaggcgccc
     5941 cccggcgagc ccgtcacggt cgggttcctc gaggcgaccc ggggcttcgc gctcgtgctg
     6001 ttcgcgctgt tcggcttcac cgcgttcggc gtcgggtggt tcctcgcggg tgacaccgtg
     6061 aagaaatggt tcgcgcgcat caaggcgcga agctgagggt ggccggctca gcccccgatc
     6121 gcggacatcg aacgctccgg ctgctcgaag tccgggaggt tgatccggtg cccggaccac
     6181 ttccgccaca cggtgcgacg gatcaggtcc accagctcgt cgtcggtggc gccctcgcgg
     6241 agcg
//
LOCUS       DATBRK010000388         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1953958, whole genome shotgun sequence.
ACCESSION   DATBRK010000388 DATBRK010000000
VERSION     DATBRK010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953958"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1050
                     /locus_tag="VIH70_11440"
     CDS             43..1050
                     /locus_tag="VIH70_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8117018.1"
                     /translation="MRIGLALPHYDFSIPGVSPIRFEALAEVAVRAERLGFDSVWVSD
                     HLFASLRRYGGGDERYGSLEPLSTLAALAPLTERVRLGTLVLSAGFRHPAIVAKTVTA
                     IDRLSDGRVELGIGAGWFADEYRAFGYAFGSVGERFAVLEETVGYLDALFGDEPASFE
                     GERFALRGGYNHPRPVQEPRPPLLVGGKGGPRMLRLAARFADGWNTVWRWTPEAYGER
                     AAAAREACEREGRDPATLRLSLGLFTVVGEDEADLVRRYEVMRERLPPGVVDAAPLEE
                     LRRDGLVGTPADVLERLERFAALGVGELVVGPAPVWFALPDPSMLDLLAERVLPAAKA
                     L"
     gene            1050..1268
                     /locus_tag="VIH70_11445"
     CDS             1050..1268
                     /locus_tag="VIH70_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117019.1"
                     /translation="MEPLDAAIAVLREEGQPLHWTRIQDLALRRGYLDPFEVPEVRRT
                     LLAALADGVRSGVLEKEGTGVYAVSPDR"
     gene            complement(1340..2332)
                     /gene="glpX"
                     /locus_tag="VIH70_11450"
     CDS             complement(1340..2332)
                     /gene="glpX"
                     /locus_tag="VIH70_11450"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY8117020.1"
                     /translation="MEERPDRNIALELVRVTEAAALAAGRWVGRGDKVAADQAAVDAM
                     RLMIDSVSMDGVVVIGEGEKDEAPMLYNGESVGTGEGPACDVAVDPIDGTRLTAVGQP
                     NALSVIALAERGSMFFPGVAVYMEKVATGPEAADAIDVTASPEENVRLVAKAKGIRPE
                     EVGVVILDRERHAEMITRVREVGARVHLITDGDVAGAIAAATTRTSGLDLLVGIGGTP
                     EGVVAAAALKCLGGAMQGRLFPRDDDERRRLLDTGYDIERVLTTDDLVAGQDVFFAAT
                     GITDGSLLRGVKYWPDGATTFSMVMRNRSGTVRMIEAEHRFEKLERFSRIAYRR"
     gene            complement(2407..3417)
                     /locus_tag="VIH70_11455"
     CDS             complement(2407..3417)
                     /locus_tag="VIH70_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8117021.1"
                     /translation="MTEPVLVTGGTGVVGRGLLRRLVADGRNVRALARSNASADAVGA
                     LGAEAVRGDVLDLAWLTEAMRGCSTVFHIAGVNAMCLRDPIDMVRTNVEGSATMVRAA
                     AAADVGRVVYTSSSSAIGEPRHTVGREDSPHRGSYLSAYERSKHVAERRVFELAGALD
                     VPVVSVNPSSVQGPGRATGSARLLLEVVSGRLPVLVDTTISLVDIEDCARGHVLAETR
                     GTPGERYLLSGASLTTREAVDLLDRIWGLPRKVRFAPGWVATVGGWIAWAGARALRRD
                     ATVCPEAVRTLLHGHRYDGSRAARELGLRYTPVETTLRRTLEWCEERGLVPPARERRP
                     HT"
     gene            complement(3414..>3864)
                     /gene="pgsA"
                     /locus_tag="VIH70_11460"
     CDS             complement(3414..>3864)
                     /gene="pgsA"
                     /locus_tag="VIH70_11460"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY8117022.1"
                     /translation="ALFAVAALTDFFDGLLARRWAQTSVFGNFLDTTADKLLVAGALI
                     GLLVVGRVSPWIAVIIIGREILIIGLKGAVASTGDLVRPSVWGKAKANVQFLAIFLAI
                     LRPGDPIGTLYLDQWAMIAAAVITVLSGLEYVVRFRGSITSGRGPAA"
BASE COUNT          523 a         1388 c         1398 g          555 t
ORIGIN      
        1 gcggacgtgc tgcgtcacct ctagcgatgc gggcctgacc ggatgcggat cggcctggcg
       61 ctcccgcact acgacttctc gatcccgggg gtgtcgccga tccggttcga ggcgctcgcg
      121 gaggtcgccg tccgggccga gcggctcggg ttcgacagcg tgtgggtgtc ggatcacctg
      181 ttcgcgtcgc tgcgtcgcta cgggggcggg gacgagcgtt acgggtcgct cgaaccgctc
      241 tcgacgctcg cggcgctcgc gccgctgacc gaacgggtgc ggctggggac gctggtgctg
      301 tcggcggggt tccgccaccc cgcgatcgtc gcgaagacgg tcacggcgat cgaccgcctc
      361 tcggacggcc gcgtcgagct ggggatcggg gccggatggt tcgccgacga gtaccgggcg
      421 ttcggctacg cgttcggatc ggtcggggag cggttcgcgg tcctcgagga gaccgtgggc
      481 tacctggatg cgttgttcgg ggacgagccg gcgtcgttcg agggggagcg gttcgcgctc
      541 cgtggggggt acaaccaccc caggccggtg caggaaccgc ggccgccgct gctcgtgggc
      601 ggcaagggtg gtccgcggat gctgcggctc gccgcgcggt tcgccgacgg ctggaacacg
      661 gtctggcgat ggacgcccga ggcctacggg gagcgggcgg ccgccgcgcg cgaagcgtgc
      721 gagcgtgaag ggcgcgatcc cgcgacgctg cggttgtccc tcggcctgtt caccgtcgtc
      781 ggcgaggacg aggcggacct cgtccgccga tacgaggtga tgcgggaacg gttaccaccg
      841 ggcgtcgtcg acgcggcgcc gctcgaggag ctgcgacggg acgggctggt cggcaccccc
      901 gccgacgtgc tcgaacgcct cgagcggttc gccgcgctcg gcgtgggcga gctggtcgtg
      961 ggtcccgcgc cggtctggtt cgcgcttccg gatccgtcga tgctcgacct gctggccgaa
     1021 cgcgtgctcc ctgcggccaa ggcgctctga tggaaccgct cgacgcggcg atcgcggtgc
     1081 tgcgggagga ggggcagccc ctgcactgga cgaggatcca ggacctggcg ctgcgtcgtg
     1141 gatacctcga cccgttcgag gtccccgagg tccgcaggac gctgctcgcg gcgctcgccg
     1201 acggcgtacg atcgggcgtg ctcgagaagg aaggcaccgg cgtgtacgcg gtctcgcccg
     1261 accgatgagc tcgaagacgc tcgcgctcgc cgcgtcgcca tcgggcgcga ggtcgcgggt
     1321 ggcgcggcgg aagacctgat cagcgccggt acgcgatcct ggagaaccgt tcgagcttct
     1381 cgaaccggtg ctccgcctcg atcatccgga ccgtgcccga ccggttgcgc atcaccatcg
     1441 agaacgtggt cgcgccgtcg ggccagtact tgacgccgcg cagcagcgac ccgtcggtga
     1501 tcccggtggc cgcgaagaac acgtcctgcc cggcgacgag gtcgtcggtc gtgaggacgc
     1561 gttcgatgtc gtaccccgtg tcgagcagcc gtcgccgctc gtcatcgtcg cgcgggaaca
     1621 ggcgcccctg catcgctcct ccgaggcact tcaaggccgc ggcggcgacg acgccctcgg
     1681 gcgtcccgcc gatcccgacc aggagatcga gtcccgaggt gcgtgtcgtg gcggccgcga
     1741 tcgcgccggc gacgtcgccg tcggtgatca ggtggacgcg cgcgccgacc tcgcggacgc
     1801 gggtgatcat ctcggcgtgg cgttcgcggt ccaagatcac gacgccgacc tcctcggggc
     1861 ggatgccctt ggccttggcg accaggcgaa cgttctcctc gggcgaggcg gtgacgtcga
     1921 tcgcgtcggc cgcctccggg ccggtcgcga ccttctccat gtagacggct acgccgggga
     1981 agaacatcga accgcgttcg gccagcgcga tcaccgagag cgcgttcggc tgtccgacgg
     2041 cggtcaggcg cgtcccgtcg atcgggtcga cggcgacgtc gcacgccggt ccctcgccgg
     2101 tcccgaccga ctcgccgttg tagagcatcg gggcctcgtc cttctcgccc tccccgatca
     2161 cgacgacgcc gtccatcgag accgagtcga tcatcaggcg catcgcgtcg accgcggctt
     2221 gatccgccgc gaccttgtcg ccccggccca cccatctgcc ggcggcgagc gccgcggcct
     2281 cggtgacccg gacgagctcc agtgcgatgt tgcggtccgg acgctcctcc atcgcgcgat
     2341 ccccccggtt cgagcccgtc gtccgttcgt ccctcgggtc gtccctcggg tcctgccgag
     2401 gccatcctac gtgtgcggcc gccgctcgcg cgcgggcggg accaggcctc gttcctcgca
     2461 ccattcgagc gtgcgtcgca gcgtcgtttc gaccggcgtg tagcgcaggc cgagctcgcg
     2521 cgccgccctc gagccgtcgt accggtggcc gtggagcaac gtccggacgg cctcgggaca
     2581 cacggtcgcg tcgcgacgga gtgccctcgc acccgcccac gcgatccatc cgcccaccgt
     2641 cgcgacccag cccggcgcga accgcacctt tcgggggagc ccccagatcc ggtcgagcag
     2701 gtcgaccgcc tcgcgcgtcg tcaggctcgc cccagacagc aggtaccgct caccgggcgt
     2761 tccgcgcgtc tcggccagaa cgtggcccct cgcgcagtcc tcgatgtcga cgagcgagat
     2821 cgtcgtgtcg acgagcacgg gaagccgtcc gctcacgacc tcgagcagga ggcgcgccga
     2881 gccggtcgcc cgcccgggcc cctggacgga agatgggttc accgacacca ccgggacgtc
     2941 gagcgccccc gcgagctcga agacgcgtcg ctcggccacg tgcttggagc gctcgtaggc
     3001 ggagaggtac gagcctcgat gaggcgagtc ctctcgcccc accgtgtgcc ggggctcgcc
     3061 gatcgccgac gaggacgatg tgtagacgac gcgtccgacg tcggcggcgg cggcggcgcg
     3121 gaccatcgtc gccgagccct cgacgttggt gcggaccatg tcgatcggat cccgcaggca
     3181 catcgcgttg acgcccgcga tgtggaagac ggtcgagcag ccgcgcatcg cctcggtcag
     3241 ccacgcgaga tccagcacgt cgccccggac cgcctcggca ccgagcgcgc cgaccgcgtc
     3301 cgcggaggcg ttcgaccgcg ccagtgcgcg gacgtttcgg ccgtcggcga cgaggcgccg
     3361 caacagaccc cgcccgacga caccggtccc gccggtgacc aggaccggtt cggtcacgct
     3421 gcggggccgc ggcccgacgt gatcgacccg cggaaccgca cgacgtactc gagaccggag
     3481 agcacggtga tgacggccgc cgcgatcatc gcccactgat ccaggtacag cgtgccgatc
     3541 ggatcgccgg gacgcaggat cgcgaggaag atcgcgagga actgcacgtt ggccttcgcc
     3601 ttgccccaga ccgacgggcg gacgaggtct ccggtcgacg cgaccgctcc cttcaggccg
     3661 atgatcagga tctcgcggcc gatgatgatg acggcgatcc acggcgacac ccgtccgacc
     3721 accagcaggc cgatcagcgc gcccgcgacc agcaacttgt ccgccgtcgt gtcgaggaag
     3781 ttgccgaaca cgctcgtctg cgcccagcgg cgggccagca gcccgtcgaa gaagtcggtg
     3841 agcgcggcga ccgcgaacag cgcc
//
LOCUS       DATBRK010000389        11710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2099888, whole genome shotgun sequence.
ACCESSION   DATBRK010000389 DATBRK010000000
VERSION     DATBRK010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11710
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099888"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VIH70_11465"
     CDS             complement(<1..555)
                     /locus_tag="VIH70_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020556171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY8117023.1"
                     /translation="MDAWQPPSSLPPAEGPLVDTFGRVADDLRVSVTDRCNFRCTYCM
                     PAEGLVWLPKHEILTFEELTRLLELFVHRLGIRSIKVTGGEPTVRADLPELIRMFRSS
                     APEIDLSMTTNGVLLERLAAPLAEAGLDRVTVSLDSLLRHRFEQMTRRDALDRVLAGI
                     AAAEEAGLVPIKINCVVIAGTNDDE"
     gene            complement(569..841)
                     /locus_tag="VIH70_11470"
     CDS             complement(569..841)
                     /locus_tag="VIH70_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HEY8117024.1"
                     /translation="MDAWIDRLAAALDEDPLTDAETTRLLGSARDVAHRVERKITPLA
                     AFLLGAATGTRMAEGVSRDDALGSALGILEGLLPSEPPEGRTTSGE"
     gene            909..2144
                     /locus_tag="VIH70_11475"
     CDS             909..2144
                     /locus_tag="VIH70_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HEY8117025.1"
                     /translation="MSGIRAAVVTVSDGVSQGTRADGSGDAAEALLRDAGFDVEARTA
                     LPDERPAIEVALRDLADVRGFALVVTTGGTGFGPRDVTPEATLAVLEREAPGLAELMR
                     RDGLRQTPMAALSRAVAGSRGATLIVNLPGSPKGVRECLGALLPVLPHAVELLGGRTG
                     AHPTGHADGNEAKRPAPAVVGSVEAKAVKVHGSPPCAVGNSMRIVPGGEVHGTLGCAE
                     FDEAAVRDAAEILASGEPGTRTYHHELGDIEVYLEPSRRAPALVVVSATDVARELARL
                     APGLGFAPILVETRPERLTDADANSMPVARDLGEVKIDAETRLVFTDHDAPGIAAALA
                     GALRSPARFIGVMGSRRHVGPYVEELRSMGFGDEELARIRSPLGLDLGGRRPAEVALS
                     IAAGLVADEHDRAAGWLDR"
     gene            2141..2956
                     /locus_tag="VIH70_11480"
     CDS             2141..2956
                     /locus_tag="VIH70_11480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8117026.1"
                     /translation="MSTARRSRPVTPVGASRPSAPAQPAYIRRNRANWNREADDYQRE
                     HGPQLNRFDRPAWGTWGIPESELGILGDVAGRRVLELGCGGGQWSIVLARRGARAVGL
                     DLSIRQLAHARRLAGGASQPVPFVNANAERAPFADGSFDIVFCDHGAMTFADPYLTVP
                     EVARLLRPGGAFAFNIASPLIWLCWGDGEQPPGPELVRSYFGLRRATWETVEFQLAYG
                     EWIRLFRSSGFEVLDLVELRPPARPRTTYPDFAPVGWARRWPGENIWKLRKLG"
     gene            2953..4377
                     /locus_tag="VIH70_11485"
     CDS             2953..4377
                     /locus_tag="VIH70_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY8117027.1"
                     /translation="MSARGVASSPAPAGPGVRRGLAVGLAVLALAWSVAALPEVRGTE
                     DPRPNVIVIVTDDQSDDSIPNSYEVMPFLQRRALDPRDHWIVFENGYVNTPLCCPSRA
                     TMLTGRFSHHTAVRDNEDAHLFDDSSTLATWLHDAGYHTGLVGKYLNLYPFDGPAFVP
                     AGWERWWGREHGPVTSLYYDYSMFEQDHVVRYGDTDADYATDVLADQATEFVREAPAD
                     RPYFLWFAPTAPHPPWVAAPRDEGAFSGLAVPTPPSVGEPDVSDKPAWVRALPPMGDA
                     QRSAQREHRRASYAALLAVDDAIRRIMNAVRARGDLARTVVVFTSDNGLAFGEHRWTR
                     KSCPYQACLGVPFMIRIPGVPHRTERAIVSVADIAPTIAELSGASTPTAFDGVSLVRL
                     LSTGSREGLPGEVFAEWVGDRAIPAWWEIRTQRFAYIELGTGERELYALRDDPFELVN
                     VVDAPAFEGDVSRLAATLEAYRSA"
     gene            4374..5888
                     /locus_tag="VIH70_11490"
     CDS             4374..5888
                     /locus_tag="VIH70_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY8117028.1"
                     /translation="MRHGAAIALGALLLVAPAEPAGRASAATARVPAVSETRAPPDIV
                     LIVTDDQRWDTLWAMPILSERLAGAGVTFPDAFVVNPLCCPSRASILTGDYSHTHLVY
                     RQIPPFGRFEWFHDGSTLATWLHDAGYRTGLFGKYLDGYQHAAVTGYVPPGWDRWVAF
                     VHSAPVDYTLTIDGSLRGFGHGPQDHATEVLADEAVAFVEGSTGPMFLELATSAPHEP
                     AIPSPGDEDAFPDLPPARPPSFDEADVSDKPAWVRDLPQLTASQEAAIDAFRADQYRS
                     LLGVDRAVGRVLDALERTGRLENTLIVFTSDNGILHGEHRWTKKEAPYEGSIRVPLVM
                     RWDAAGWTPGTRLPGVLALNIDLAPTIADAAGVPHPPTDGRSLLPLLGGERGSWRSDF
                     LIEHMEGTNPIPTFCAVRSERWTYVRYSTGEEELYDLVADPFELDNLAGVAAMRPVLD
                     DRRARLRELCSPVPPGFGDRSGTSVPIALAVLGGLVLVESLASRRTRHPRRAPR"
     gene            5956..7566
                     /locus_tag="VIH70_11495"
     CDS             5956..7566
                     /locus_tag="VIH70_11495"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8117029.1"
                     /translation="MPIPRRRLRFDRRRAARRRGLAAFLGVVVVAIAALGWASSRGDE
                     DGASTGPSGGPSPATGSNGGTGAGGSSGNGGGTAGNGGGIIVPGETPIEHVVFIVKEN
                     RTFNNYFATYPGAVGATEGGTIRCTQDGCHDGPVVRLTRGPDIYPHDLTHCFRCGLTA
                     INGGKMNGFNRMNGPIPQSEIADLYGADETNYSYLDRKDIPNYWAYADRFVLADHFFT
                     PMYGPTLPEHLYAVAAQSNFIVDNKSTTDHEGNYCDDPTERATRFVPHEARQHQDQIM
                     RLERAITENGSNVYDIADFWGSIRLCFDIPVLPDQLEREGISWKYYASENAWMNVMQM
                     IRHVRYGPMWRNVREPSDFVRDVKRGEMPAVSWVIPPESYNEHPGAGKSTCAGENWTV
                     HQVNAIMRSEFWRSTAIVVVWDDFGGFYDPVEPPQYDVMGLGPRTPALIISPYTRQGS
                     NPDGGVVDDTTYEFSSVLAFIERLFGLEPMTDRDGRADPLSGAFDFEHPNFDKLVLPL
                     RDDCPYGTSTSEFAAGWPMLRTIGKPGD"
     gene            complement(7563..8906)
                     /locus_tag="VIH70_11500"
     CDS             complement(7563..8906)
                     /locus_tag="VIH70_11500"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8117030.1"
                     /translation="MPKTLLRLSAVAFLSALLGSSCADDAPEWTPLPPLQYGEAPAAV
                     NADAVETATPIKHVVFVIKENRTFDNMFGLFPGANGVTVGMDRGEPRALTPAVDRLDM
                     DIRHCYDCALQAWNEGKMDGFATISETADEYAYTQFRPEDLPNYWHWASDYVLGDRFF
                     ASAQGPSFPNHLYTIAAQSGGTHENVYQDIDELRERHRNTGLFKAWGCDSMQDGYAVV
                     VDSEGVEKKVPPCFDFLTEGDLLTEARIPWAYYAATQYQNGYLWSAYDAIRHVRENEA
                     SWQRHMFPVDSFAQHARSGLLPPVTWVTPRFELSEHPEYSMCHGENWTTEIVNAVMDS
                     PEWDSTAIFITWDDYGGFYDHVPPPQVDDFGFGIRVPLLVISPYAKRGFVSHELGEFS
                     SVLRFIEDNWGLTQLTRRDRDATPLLSAFDFGQAPRPPDPLPLRTDCVGPEFPDD"
     gene            9015..9494
                     /locus_tag="VIH70_11505"
     CDS             9015..9494
                     /locus_tag="VIH70_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117031.1"
                     /translation="MRRFVMVLWAVMVGILAAGLTTAFAGTRIVQEETVVFGEHTLRG
                     RNIDLVGRANDFRPGDRYIFRSELTDAQDAVAGHLFVDCSVQFAKQDSCSQIYEIPAR
                     GTVTVEGLIPVSQLEVGGTWVLAITGGTGEFENVRGSVTVVIVDDRGNSEHSLHLLP"
     gene            complement(9516..10931)
                     /locus_tag="VIH70_11510"
     CDS             complement(9516..10931)
                     /locus_tag="VIH70_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEY8117032.1"
                     /translation="MDEHGDPGARRPRHDAGHAHAAEQPFEYPLHREYVEPDWTRLPG
                     FRDVTKEQWESAQWQRAHTIKNLVELKRALGDLLSDHLYADIERDQRERATMSMLLPP
                     QMVNTMDETDLYADPVRRYMAPALSDRAEEFPSHPMAARDSLHEADMWAVEGLTHRYP
                     TKVLAELIPTCPQYCGHCTRMDLVGNDTTQVLKYKFETKQNERWDAMLDYLRRTPSVR
                     DVVVSGGDMANVPIRRLQEWLFGLFDIENVRDVRIATKGLMGIPQHFLQDDVLAGYDA
                     IAKRAHERGVEVAIHTHVNAAQQVTPLVGAAVRRLLDMGYRDVRNQGVLLRGVNTTPR
                     QILELSFMLLDHAKVMPYYFYLCDMIPNSEHWRLAVWEAQELQFAIMGYLPGFATPRF
                     VCDVPLVGKRWVHMVESYDRVKGISYWRKNYRTGIEYDDPEALTREYPYYDPITTLPE
                     EGKRYWREFQAGRRGKELQPA"
     gene            complement(11105..11395)
                     /locus_tag="VIH70_11515"
     CDS             complement(11105..11395)
                     /locus_tag="VIH70_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117033.1"
                     /translation="MADYKEVRVERLFLKVPNTEKLRKGAAGRHKHLLADGWREMERR
                     REIDHIWVRYERTGPAPLRLRMPRPEKDQGRFERRPRMGGRDGGRGGPRGRR"
BASE COUNT         1688 a         4052 c         4149 g         1821 t
ORIGIN      
        1 ctcgtcgtcg ttggtccccg cgatcacgac gcagttgatc ttgatcggga ccaggcccgc
       61 ctcctcggcg gccgcgatgc ccgcgagcac ccggtcgagc gcgtcgcgac gggtcatctg
      121 ctcgaaccgg tgccgcaaca acgagtcgag cgagaccgtc acgcggtcca gtcccgcctc
      181 ggcgagcggc gcggcgagcc gctcgagcag cacgccgttc gtcgtcatcg acaggtcgat
      241 ttcgggcgcg gagcttcgga acatccggat cagctcgggg aggtcggctc gcacggtggg
      301 ttcgccaccg gtgaccttga tcgagcggat ccccagccgg tggacgaaca gctcgaggag
      361 cctcgtgagc tcctcgaagg tcaagatctc gtgcttgggg agccagacca gtccctcggc
      421 gggcatgcag taggtgcacc ggaagttgca tcgatcggtc accgagaccc ggaggtcgtc
      481 cgcgacccgg ccgaacgtgt cgaccagggg gccctccgcc ggggggaggg atgacggcgg
      541 ctgccaggca tccatgcgga cctccagcct actcccctga ggtcgttcgg ccctcgggag
      601 gctcggacgg caggagcccc tcgaggatcc cgagcgccga tcccagggcg tcgtccctcg
      661 agacgccttc ggccatcctc gtgccggtcg ccgcgccgag gaggaacgcc gcgagcggcg
      721 tgatcttgcg ttccacgcgg tgcgcgacgt cccgggccga gccgagcaag cgggtggtct
      781 cggcgtcggt cagggggtcc tcgtcgagcg ccgccgcgag tcggtcgatc cacgcgtcca
      841 tcggtgctcc cttctgtccg ggcaaccgta gtacggacga gcgcgggcgc gtctggcagg
      901 atgggtgcgt gagcggcatc cgcgccgcgg tcgtgaccgt ctccgacggc gtctcccagg
      961 ggacgcgagc cgacggctcg ggcgacgccg ccgaggcctt gctgcgtgac gcggggttcg
     1021 acgtcgaggc tcggaccgcc ctgcccgacg agcgcccggc gatcgaggtc gccctgcgcg
     1081 acctcgcgga cgttcgcggg ttcgccctcg tcgtcaccac cggcgggacg gggttcggcc
     1141 cgcgtgacgt gacgcccgag gccacgctcg cggtgctcga acgcgaggcc cccggtctgg
     1201 ccgagctgat gcgccgcgac ggcctgcgac agacgccgat ggcggccctg tcgagagccg
     1261 tggcgggctc gcgcggcgcg acgctgatcg tgaacctccc cggcagcccg aagggcgtcc
     1321 gcgagtgcct cggggccctg ctgccggtgt tgccgcatgc cgtcgagctg ctcggcggtc
     1381 gaacgggggc gcatccgacg ggtcacgccg acgggaacga ggcgaagcgg cccgcgcccg
     1441 cggtggtcgg ctccgtggag gccaaggcgg tcaaggtgca cggctcgccg ccgtgcgcgg
     1501 tcggcaactc gatgcggatc gtccccgggg gtgaggtgca cgggacgctc gggtgcgccg
     1561 agttcgacga ggcggccgtc cgcgacgccg ccgagatcct cgcgtcgggc gaacccggga
     1621 cccgcacgta ccaccacgag ctcggcgata tcgaggtgta cctcgaaccg tcgcggcgcg
     1681 cgccggccct cgtggtcgtc tcggccaccg acgtggcccg cgagctggcg cggctcgcgc
     1741 ccggcctcgg gttcgcgccc atcctggtcg agacgcgtcc ggaacgcctc accgatgccg
     1801 atgcgaactc gatgccggtc gcgcgcgacc tcggcgaggt gaagatcgac gccgagaccc
     1861 gcctggtctt caccgaccac gacgcgccgg ggatcgcggc ggcgctcgcc ggcgccctgc
     1921 gttcgccggc gcggttcatc ggcgtgatgg ggagccgccg ccacgtgggc ccgtacgtcg
     1981 aggagctgcg ctcgatggga ttcggcgacg aggagctcgc gcgtatccga tcgccgctcg
     2041 gcctcgacct cggcggacgc cggccggccg aggtcgccct gtcgatcgcc gcgggactgg
     2101 tcgccgacga gcacgatcgc gctgccggat ggctcgaccg gtgagcaccg ctcgccggag
     2161 cagacccgtc acgcccgtgg gcgcctcccg tccgagcgcc ccagcccagc ccgcctacat
     2221 ccgccgcaac cgcgcgaact ggaaccgcga ggcggacgac taccagcgcg agcacggccc
     2281 gcagctcaac cgcttcgacc gtccggcgtg gggaacctgg ggaatcccgg agtccgagct
     2341 cggcatcctc ggcgacgtcg ccgggcggcg agtgctcgag ctcgggtgcg gcggcggcca
     2401 gtggtcgatc gtgctggcgc gacggggagc tcgcgcggtg gggctggatc tgtcgatccg
     2461 ccagctcgcc cacgcccgcc ggctcgccgg tggggcctcg cagccggtgc cgttcgtgaa
     2521 cgccaacgcc gagcgcgcgc cgttcgcgga cgggagcttc gacatcgtgt tctgcgatca
     2581 cggggccatg acgttcgccg atccgtacct gacggtgccc gaggtcgcgc ggctcctgcg
     2641 tccgggcggt gcgttcgcct tcaacatcgc gagcccgctg atctggctgt gctggggcga
     2701 cggcgagcaa ccgccgggtc ccgagctggt ccgcagctac ttcggcctgc gccgggccac
     2761 gtgggagacg gtcgagttcc agctggccta cggcgagtgg atccggctgt tccggtcgag
     2821 cggcttcgag gtactggacc tggtcgagct ccggccaccc gcccgtcccc ggaccacgta
     2881 tccggacttc gcgccggtcg ggtgggcccg tcggtggccc ggcgagaaca tctggaagct
     2941 ccggaagctc ggatgagcgc gcggggggtc gcgtcgagcc ccgcaccagc gggtcccggc
     3001 gtccgccgcg gcctggcggt cgggctcgcc gtcctcgccc tggcgtggag cgtggccgcg
     3061 ctcccggagg ttcgtggcac ggaggacccc cgaccgaacg tgatcgtgat cgtgacggac
     3121 gatcagtccg acgactcgat cccgaactcg tacgaggtga tgccgttcct tcaacgccgg
     3181 gcgctcgatc cgagggacca ctggatcgtg ttcgagaacg ggtacgtcaa caccccgctg
     3241 tgctgcccgt cgcgggcgac gatgctcacc ggacggttct cgcatcacac cgcggtccgg
     3301 gacaacgagg acgcacacct gttcgacgac agctcgacgc tcgcgacgtg gctgcacgat
     3361 gcgggctacc acaccgggct ggtcggcaag tacctgaacc tgtacccgtt cgacgggccg
     3421 gcgttcgttc cggccgggtg ggagcgctgg tgggggcgtg agcacggtcc ggtgacgagc
     3481 ctgtactacg actactcgat gttcgagcag gaccacgtcg tccggtacgg ggacaccgac
     3541 gccgactacg cgaccgacgt cctcgccgat caggcgaccg agttcgtccg cgaggcaccg
     3601 gccgatcgac cctatttcct ctggttcgcc ccgacggcgc cacacccccc ctgggtcgcg
     3661 gcgccccggg acgagggggc gttctcgggg ctcgcggtgc ccacgccgcc gtccgtgggg
     3721 gagcccgacg tctccgacaa gccggcctgg gtccgcgctc tccccccgat gggcgacgcg
     3781 cagcgatcgg cgcagcgtga gcaccgcagg gcgtcgtacg cggcgctcct cgcggtcgac
     3841 gacgcgatcc gccggatcat gaatgcggtc cgcgcccgcg gtgacctcgc ccgcacggtc
     3901 gtggtcttca cctcggacaa cgggctcgcg ttcggggaac atcgctggac gaggaagagc
     3961 tgcccctacc aggcctgcct tggcgtcccc ttcatgatcc ggatacccgg cgtcccgcat
     4021 cggaccgagc gagcgatcgt ctccgtcgcc gacatcgccc cgacgatcgc cgagctctcg
     4081 ggcgcgtcga ccccgacggc gttcgacggc gtcagcctcg tgcggctgct ctcgaccggt
     4141 agccgggagg ggttgccggg cgaggtgttc gccgaatggg tcggtgaccg ggcgatcccc
     4201 gcgtggtggg agatccgcac tcagcggttc gcctacatcg agctcgggac gggagagcgt
     4261 gagctctacg cgctccggga cgatcccttc gagctcgtga acgtggtgga tgctccggca
     4321 ttcgagggcg acgtgtcgcg actggccgcg accctcgagg cgtaccggag cgcgtgaggc
     4381 acggcgccgc gatcgccctc ggggcgttgc tgctcgtcgc cccggccgag cccgccggcc
     4441 gggcctcggc ggcgaccgct cgcgtgccgg ccgtctccga gacgagggcg ccgcccgaca
     4501 tcgtcctgat cgtgaccgac gatcaacgat gggacaccct gtgggcgatg ccaatcctgt
     4561 cggagcggct ggccggcgcc ggcgtgacgt tccccgacgc cttcgtcgtc aacccgctct
     4621 gctgccccag ccgcgcgagc atcctgaccg gcgactactc ccacacccac ctcgtctatc
     4681 ggcagatacc gccgttcggc cggttcgagt ggttccacga cggctcgacg cttgcgacgt
     4741 ggctgcacga cgcgggctac cggaccgggc tgttcggcaa atacctcgac gggtatcagc
     4801 acgcggcggt gaccgggtac gtcccgcccg ggtgggaccg atgggtggcc ttcgtgcatt
     4861 cggcaccggt ggactacacc ctgacgatcg acggctcgct gcgcgggttc gggcacggtc
     4921 cgcaggacca cgcgaccgag gtgctcgccg acgaggcggt cgcgttcgtg gagggctcga
     4981 ccgggccgat gttcctggag ctcgccacgt cggcgccgca cgagcccgcg atcccgtccc
     5041 ccggcgatga ggacgccttc cccgacctgc ccccggcgcg gccgccgtcg ttcgacgagg
     5101 ccgacgtgtc ggacaagccc gcgtgggtcc gcgacctgcc acagctgacg gcctcgcagg
     5161 aggcggcgat cgacgccttt cgggcggacc agtaccggtc cctgctcggc gtggaccgcg
     5221 cggtcggtcg ggtcctcgac gcgctcgagc gcaccgggcg gctggagaac acgctgatcg
     5281 tgttcacctc ggacaacggg atcctgcacg gggagcatcg gtggaccaag aaggaggcgc
     5341 cgtacgaggg atcgatccgc gtcccgctcg tgatgcgttg ggacgcggcc gggtggacgc
     5401 ccgggaccag gctgcccggc gtcctcgccc tgaacatcga cctcgcgccg acgatcgccg
     5461 acgcggccgg cgtcccccat cccccgaccg acggccggag cctcttgccg ttgctcgggg
     5521 gtgaacgggg ctcgtggcgc tcggatttcc tgatcgagca catggagggc acgaacccga
     5581 tccccacgtt ctgcgccgtg cgttcggagc gctggacgta cgtgcggtac tcgacgggcg
     5641 aagaggagct ctacgacctc gtcgccgacc cgttcgagct cgacaacctg gccggcgtcg
     5701 cggcgatgcg gccggtgctc gacgaccgac gagcccgcct gcgcgagctc tgctcgccgg
     5761 tcccgccggg gttcggggat cgatccggaa ccagcgtccc gatcgcgctg gcggtgctgg
     5821 gcgggctcgt gctggtcgag tcgctggcgt cgcggagaac gcgccaccct cggagggcgc
     5881 ctcgataggc taggcggcca tgagcgcacc atccggaccg cggccgtgag tgcaccgtcc
     5941 ggacccggtt cgcgcgtgcc gatcccacgg aggcggctgc ggttcgaccg acggcgggct
     6001 gcgcggcggc gtggcctcgc ggcgttcctc ggcgtcgtcg tggtggcgat cgcggcgttg
     6061 ggatgggcgt cgagccgcgg agacgaggac ggggcatcga ccggaccgtc gggcgggccg
     6121 tcgcccgcga ccggttcgaa cggtgggacg ggcgccgggg ggtcctccgg caacggtggc
     6181 gggaccgcgg gcaacggcgg cgggatcatc gtccccggcg agacgccgat cgagcacgtc
     6241 gtgttcatcg tcaaggagaa ccgcacgttc aacaactact tcgcgaccta cccgggtgcg
     6301 gtcggcgcga ccgagggcgg cacgatcagg tgcacgcagg acgggtgcca cgacggcccg
     6361 gtcgtccggc tcaccagggg tcccgacatc tatccccacg atctgacgca ctgcttccga
     6421 tgcggcctca ccgcgatcaa cggtggcaag atgaacggct tcaaccggat gaacggcccg
     6481 atcccacaat cggagatcgc cgacctgtac ggcgcggacg agacgaacta ctcctacctc
     6541 gatcgcaaag acatcccgaa ctactgggcc tatgccgatc gtttcgtgct ggccgaccac
     6601 ttcttcacgc cgatgtacgg tcccacgctg cctgaacacc tgtacgcggt ggcggcccag
     6661 tcgaacttca tcgtcgacaa caagtcgacc accgatcacg aggggaacta ctgcgacgac
     6721 ccgaccgaga gggcgacgcg gttcgtcccg cacgaggccc ggcagcacca ggaccagatc
     6781 atgcggctcg aacgcgcgat caccgagaac gggtccaacg tctatgacat cgccgatttc
     6841 tgggggagca tccgcctgtg cttcgacatc cccgtgctgc ccgaccagct cgagcgggag
     6901 ggcatctcgt ggaagtacta cgcgagcgag aacgcgtgga tgaacgtcat gcagatgatc
     6961 cgccacgtcc ggtacgggcc gatgtggcgc aacgttcggg agccctcgga cttcgtcagg
     7021 gatgtgaagc ggggcgagat gcccgcggtc tcgtgggtca tcccgcccga gtcctacaac
     7081 gagcatcccg gcgccgggaa gagcacgtgc gccggagaga actggaccgt ccaccaggtc
     7141 aacgcgatca tgcggagcga gttctggcga tcgacggcga tcgtcgtggt ctgggacgac
     7201 ttcggtgggt tctacgaccc cgtcgagccg ccgcagtacg acgtcatggg gctcggtccg
     7261 cggacgccgg ccttgatcat cagcccgtac acgaggcagg gttcgaaccc cgacggcggc
     7321 gtggtggacg acacgacgta cgagttctcc tccgtgctgg ccttcatcga gcggctgttc
     7381 gggctcgagc cgatgaccga ccgcgacggg cgggccgacc cgctctcggg ggcgttcgac
     7441 ttcgagcacc cgaacttcga caagctggtg ctgccgctgc gggacgactg tccgtacggc
     7501 acctccacgt cggaattcgc cgcgggctgg ccgatgctgc gaacgatcgg caagcccggc
     7561 gactaatcgt cggggaactc cggcccgacg cagtcggtgc gcagggggag cggatccggc
     7621 ggtcgcgggg cctgcccgaa gtcgaacgcc gagaggagcg gcgtcgcgtc gcggtcgcgc
     7681 cgcgtgagct gggtgagccc ccagttgtcc tcgatgaacc tcagcacgct ggagaactcg
     7741 ccgagctcgt ggctcacgaa cccccgtttc gcgtaggggc tgatcaccag aagcgggacc
     7801 cggatcccga agccgaagtc gtcgacctgc ggcggaggca cgtggtcgta gaaccccccg
     7861 tagtcgtccc aggtgatgaa gatcgcggtc gagtcccact cggggctgtc catcaccgcg
     7921 ttcacgatct cggtcgtcca gttctcgccg tgacacatgc tgtactcggg gtgctccgag
     7981 agctcgaacc gcggggtcac ccacgtgacg ggcggcagca gccccgagcg cgcatgctgc
     8041 gcgaaggagt ccaccgggaa catgtgccgt tgccaggacg cctcgttctc cctgacgtgg
     8101 cggatcgcgt cgtacgcgga ccacaggtaa ccgttctggt actgggtcgc cgcgtagtac
     8161 gcccacggga tcctcgcctc ggtgagcagg tcgccctcgg tcaggaagtc gaagcacggc
     8221 ggcaccttct tctcgacgcc ctcgctgtcg acgacgaccg cgtacccgtc ctgcatcgag
     8281 tcgcaccccc aggccttgaa caggccggtg ttccggtgac gttcgcggag ctcgtcgatg
     8341 tcctggtaca cgttctcgtg cgtcccgccc gactgggcgg cgatggtgta gaggtggttg
     8401 gggaacgacg gcccttgcgc gctcgcgaag aacctgtctc ccaggacgta gtccgaggcc
     8461 cagtgccagt agttcggcag atcctcgggc cggaactgcg tgtacgcgta ctcgtccgcc
     8521 gtctcgctga tcgtggcgaa cccgtccatc ttgccctcgt tccaggcctg gagcgcgcag
     8581 tcgtagcagt ggcggatgtc catgtcgagg cggtcgaccg ccggcgtgag cgcccggggt
     8641 tcgccccgat ccatcccgac cgtcacaccg ttcgcgccgg ggaacagccc gaacatgttg
     8701 tcgaacgtcc ggttctcctt gatcacgaac acgacgtgct tgatcggcgt cgccgtctcg
     8761 accgcgtccg cgttcacggc cgccggagcc tccccgtact ggaggggtgg caacggggtc
     8821 cattcgggcg cgtcgtcggc gcacgagctc cccagcagcg ccgacaggaa ggcgacggcc
     8881 gacaagcgca ggagcgtctt gggcatcggt cgatgaccct atcgcagctc cgtgccgtga
     8941 gatcgttgaa ggatgcgccc ggtgacggga tgatgctcgt ccgtggtgga ggccgaggag
     9001 cgaaggagga cgtcgtgaga cggttcgtca tggtgctctg ggctgtcatg gtggggatcc
     9061 tcgcggccgg gctgacgacc gccttcgcgg ggacgcggat cgtgcaggaa gagacggtgg
     9121 tcttcggcga gcacacgctg cggggaagga acatcgatct cgtagggaga gccaacgact
     9181 tccggcccgg tgaccgctac atcttccgga gcgagctcac cgacgcccag gatgcggtcg
     9241 cgggccacct gttcgtcgat tgctcggtgc agttcgcgaa gcaggactcg tgctcgcaga
     9301 tctacgagat ccccgcccgt ggaacggtga cggtcgaggg cctgatccct gtctcgcagc
     9361 tcgaggtcgg cgggacctgg gtgctcgcca tcacgggagg caccggagag ttcgagaacg
     9421 tgcggggctc ggtgacggtc gtgatcgtgg acgacagggg caacagcgag cactcgctgc
     9481 atcttctgcc gtagtcgaag cgcccggacg cgacgtcagg ccggctgcag ctccttgcct
     9541 cgccgacctg cctggaactc gcgccagtaa cgcttgccct cctcggggag cgtggtgatc
     9601 gggtcgtagt acgggtactc gcgcgtgagc gcctcggggt cgtcgtactc gatccccgtc
     9661 cggtagttct tccgccagta cgatatcccc ttcacccgat cgtacgactc gaccatgtgc
     9721 acccagcgct tcccgacgag gggcacgtcg cagacgaacc taggggtcgc gaacccgggc
     9781 agatacccca tgatcgcgaa ctgcagctcc tgggcctccc acaccgcgag ccgccagtgc
     9841 tcgctgttcg ggatcatgtc gcacaggtag aagtagtacg gcatgacctt cgcgtgatcg
     9901 agcagcatga aggacagctc gaggatctgc ctgggggtcg tgttgacgcc gcgcaggagc
     9961 actccctggt tccgcacgtc gcggtagccc atgtccagga gcctgcggac cgccgcaccg
    10021 acgagcggcg tcacctgctg ggcggcgttc acgtgggtgt ggatcgcgac ctcgacccct
    10081 cgctcgtgcg cccgcttcgc gatggcgtcg tacccggcca gcacgtcgtc ctgcaagaag
    10141 tgctggggga tgcccataag ccccttggtc gcgatccgca cgtcgcgcac gttctcgatg
    10201 tcgaacagcc cgaacagcca ctcctgcagg cgcctgatcg ggacgttggc catgtcgccg
    10261 cccgagacga cgacgtcgcg gacgctcggc gtcctgcgca ggtagtcgag catcgcgtcc
    10321 cagcgttcgt tctgcttggt ctcgaacttg tacttcagga cctgggtcgt gtcgttgccg
    10381 accagatcca tccgcgtgca gtgcccgcag tactgcggac aggtcgggat cagttcggcg
    10441 agcaccttgg tcgggtagcg gtgcgtgagc ccctcgaccg cccacatgtc ggcctcgtgc
    10501 agcgagtccc gggcggccat cgggtgggaa gggaactcct cggcccggtc cgacagcgcc
    10561 ggggccatgt aacgacggac cgggtccgcg tacagatcgg tctcgtccat cgtgttgacc
    10621 atctggggcg ggagcagcat ggacatcgtc gcgcgctccc gttggtcgcg ctcgatgtcc
    10681 gcgtacaagt ggtcgctcag caggtcgccg agcgctcgct tgagctcgac caggttcttg
    10741 atcgtgtggg ctcgctgcca ctgggccgac tcccactgct ccttcgtgac gtcacggaac
    10801 ccgggcaggc gcgtccagtc gggttcgacg tactcgcggt gcagcgggta ctcgaacggc
    10861 tgctcggccg cgtgagcgtg gccggcgtcg tgcctcggac gtctcgcgcc tggatcgccg
    10921 tgttcgtcca tcgctcggct ccggtttccg cgacgaccgg tcgtcgcctt gtgtgacggt
    10981 gtttctaccc cgagccggat gaaccgactc tgccggtcat gatacccgcc gttcctccgg
    11041 cgtagctgcg acatcgccga aggatcttcg gctgagggaa ggattggaga agggccgtga
    11101 ccgatcagcg acggccgcgg gggccgcctc ggccgccgtc gcgtccgccc atgcggggac
    11161 gtcgctcgaa ccgcccctgg tccttctccg gccggggcat ccgtagccgg aggggcgccg
    11221 gaccggtgcg ctcgtaccgg acccagatgt gatcgatctc gcgccggcgc tccatctcgc
    11281 gccagccgtc ggcgagcagg tgcttgtgcc gaccggcggc gcccttacga agcttctccg
    11341 tgttcggcac cttgaggaac aggcgctcga cgcggacttc cttgtagtcg gccatggcgg
    11401 gaagggtagc agggcgacgc gcacgacccg agcggacgac gcggccgcgc acgggaaccg
    11461 tagactccgc gacgaacgat cgcccggcag accggccgtt ggaagagcgg ccggctgatc
    11521 cgaaggaggt tcgatgggca ccgtgaacga gtggaacgag ctggccgcac agctcggcgt
    11581 ggactcgatc aggagctcga cggcggcggg gtcgggtcat cccacgtcct cgctctcggc
    11641 ggcccacctg gtcgccgtgc tctggtccga ccacctccgc tacgacgtcg ccgacccgag
    11701 gagcctggcg
//
LOCUS       DATBRK010000390         7423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1088153, whole genome shotgun sequence.
ACCESSION   DATBRK010000390 DATBRK010000000
VERSION     DATBRK010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088153"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VIH70_11520"
     CDS             <1..1114
                     /locus_tag="VIH70_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY8117034.1"
                     /translation="EMTAFEERPHSPYYGCADATPLYVVLLDEYERWTGDRSLVRQRE
                     HEARAALAWIDDYADLLGNGYVWYRRRNEETGLENQCWKDSWDSISYRDGRIPGFPRA
                     TCELQGYAYDAKMRGARLARSVWNDPAFADRLEQEAADLKRRFNRDFWVADGGYFALA
                     LDPEGHRVDSLASNIGHLLWSGIVDKSKAKAVARNLLGPRLFSGWGVRTLAEGEGRYN
                     PIGYHVGTVWPFDNSFIAWGLRRYGFKEEAATIAEGILDAAGFFDGRLPEAFGGYERS
                     FTKYPVQYPTACSPQAWSTGAPLLVLRTMLGLEPIGDHLVVDPALPTSIGRLELLDIP
                     GRWGRMDAFGRGRVEVGSHTSRGRRNTRSRSKTRGG"
     gene            1168..2559
                     /gene="zwf"
                     /locus_tag="VIH70_11525"
     CDS             1168..2559
                     /gene="zwf"
                     /locus_tag="VIH70_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY8117035.1"
                     /translation="MTEARPADVLVIFGITGDLAKVMTFRSLYRLERRGLLDCPIVGV
                     AVDDWSVDNLKERARTSIVGTGEPLDDAIFDRLAARFSYVAGDFSDAETYKHVADTIS
                     EARAPVFYLEIPPFLFGTVIKGLHEAGLTDAARVVVEKPFGHDRESATVLAEEIHQYI
                     DESQLFRIDHYLGKMGLVEILHLRFANSILEPVWSRNFLSHVQITMAESFGVEDRGHF
                     YDPVGALRDVVVNHLMQVVAASAMEAPTRGDPTTIKDAQVNLFRAVEEADPAHYVRGQ
                     YEGYRAIDGVPDDSTTETFAALRLDIESWRWAGVPFFIRAGKRLPITQTELRLVFKHP
                     PKLGFAAFDRLPEANQLVVRLDPTTGIRLVVEAHRADAEGAAPIFLDVDFAVEGGEGP
                     TPYEVLLHAAMIGDSTRFTRQDGVEETWRVFQPLLDSPPAVQPYAQGSWGPSATDELL
                     EGLGTWHEPWVSQ"
     gene            2607..4457
                     /locus_tag="VIH70_11530"
     CDS             2607..4457
                     /locus_tag="VIH70_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY8117036.1"
                     /translation="MPSPFPPIADYAFLSDCHTGALIAPDGAVDWLCVPRFDSPSIFG
                     SMLDRQAGAFRFGPFGINVPTQIAYEPGTNVLVSTWKTPGGWVIVRDALTMGARTGED
                     KVTPHTRPPADDDADHMLVRTVQCLDGQVEIELVCEPIFDYGQVPATWSLGDGNDGVA
                     DATGGDQMFRLRTDLSLGIEGDRVRARHHLNAGERAFCALSWAHELETPSDVDDAEAR
                     IAATVRFWRTWLGRARIPDHRWREPIQRSALAIKGLTYMPTGATVAALTTSLPETPGG
                     ERNWDYRFTWVRDSTFTLQALHFLNLDWEAEEFMQFVADLEPNADGGLQIMYGIDGRR
                     DLSETTLDELSGYAGAHPVRIGNGAFDQRQNDVFGAALDSILLHTRRSQRMPRRLWPI
                     VQTQSTCATEIWREPDQGIWEARGKPQHYVSSKLMCWVAMDRAAKLAAIGGDEGLRNT
                     WQGTADEIKADILDHGVDDRGVLRQHYDTDALDASVLLAALFGFLPPEDDRLRATVLA
                     IADELTENGYVLRYKTDETDDGLSGKEGTFLICSFWLVSALAIIREWQRARDLMERLL
                     RIASPLGLFAEEFDVETGRHLGNFPQAFSHLALIEAAGRIILAERVEEIS"
     gene            4454..6001
                     /locus_tag="VIH70_11535"
     CDS             4454..6001
                     /locus_tag="VIH70_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY8117037.1"
                     /translation="MSDYDVIIIGTGAGGGTLARHLAPSGKRILLLERGGWLAREPQN
                     WSTADVFIDGRYISPDTWYDAKGKTFQPQVHYFVGGATKLYGAALYRLREKDFGELKY
                     PDGISPAWPIGYSDMEPYYTLAEQHYQVRGARGEDPTEPPSSAPYPFAALPHEPRIQQ
                     LSDDLAAAGLHPFHAPCGVLREEDAPQYSVCVRCGNCDGFPCPLHAKADAEVMGVRPA
                     LESPNVTLFTDAPAVRLNTNPEGTSVTEVVIELDGSTETVTGDIVVVSCGAANSAKLL
                     LASANDQHPKGLANGSDQVGRNYMFHYSQAVLAVSKEPNPTVFQKTLGVNDFYFGDEE
                     YEYPMGNIQMVGKSQAEMYKGEKPIETKLAPTFALRDIATHAVDFWLSTEDLPLPENR
                     VTLEKDGNVRLAYEPSNEESGKRLYHKLKSMLGHLGLHPDHLIPRNAYLKTTIPVAGV
                     AHQAGTCRMGIDPAMSVVDANCKAHELDNLYIVDTSVFPSIGAVNPALTAMANALRVG
                     DHLLERF"
     gene            complement(6009..6956)
                     /locus_tag="VIH70_11540"
     CDS             complement(6009..6956)
                     /locus_tag="VIH70_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEY8117038.1"
                     /translation="MSSESGKDVQPHPPSGEQHEVRHGDQVVVVAEVGATLRAYSAGG
                     LDVLDGFGVDEPSSAGRGQVLAPWPNRLDGGRYEFDGRRGAAAIDEPELGNAIHGLVR
                     WLPWQLASKRDDYVALECVLHPQPAYPWRLELGLQYRLVGDGLEVVALAMNASAEAAP
                     FGIGFHPYPTLGIPVDDVRLTILASRRLTTDDRALPIGEVDLAGTEFDFGVGRSVGAT
                     RLDTCFTGLARGSDGRSRARLESAEGGRGVEVWAEEAFGYLQVYTGDTLEPASRRRHA
                     VAIEPMTCPPNAFASGVDVIRLEPGARWSGVWGMAPRQG"
     gene            complement(6959..>7423)
                     /locus_tag="VIH70_11545"
     CDS             complement(6959..>7423)
                     /locus_tag="VIH70_11545"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY8117039.1"
                     /translation="GDLQARVRRWVPRFIALFTLTAAISAVWIAAGDYGVTDTFTDDV
                     WTIVFPAAALIAYLLMIVMVRRGRDFAAFFGSSAVLFLSLATISAGLYPNMLKSSTNP
                     AFSMTVSNASAEHYTLTVMLVVAIVGIPFVLLYTAGVQYLFSGKVKLTPSSY"
BASE COUNT         1246 a         2531 c         2541 g         1105 t
ORIGIN      
        1 cgagatgacg gccttcgagg agcgcccgca ctctccctac tacggttgcg cggacgccac
       61 gcccctgtac gtggtgctgc tggacgagta cgagcggtgg accggcgatc gctcgctggt
      121 ccggcagcgc gaacacgagg caagggccgc gctcgcctgg atcgacgact acgccgatct
      181 cctcggcaac ggctacgtct ggtaccggcg ccgcaacgag gagacggggc tcgagaacca
      241 gtgttggaag gactcgtggg actcgatctc ttaccgggac ggtcggatac cgggcttccc
      301 ccgggcgacg tgcgagctcc agggctacgc ctacgacgcg aagatgcgag gcgcccgtct
      361 cgcacgatcg gtctggaacg acccggcgtt cgccgaccgt ctcgaacagg aggcggccga
      421 tctcaaacgg cgcttcaacc gggacttctg ggtcgcggac ggggggtact tcgcgctcgc
      481 gctcgacccc gaggggcacc gggtcgactc cctggcgtcg aacatcggcc acctgctgtg
      541 gagcggcatc gtcgacaagt ccaaggccaa ggccgtcgcc cggaacctcc tcggtccgag
      601 gctgttctcc gggtggggcg tgcggacgct cgcggagggc gagggccggt acaacccgat
      661 cggctaccac gtggggacgg tgtggccctt cgacaactcc ttcatcgcct ggggcctacg
      721 ccggtacggg ttcaaggagg aggccgcgac gatcgcggag ggcatcctcg acgcggcagg
      781 gttcttcgat gggcgcctgc ccgaggcgtt cggcggttac gaacgctcgt tcacgaagta
      841 cccggtccag tacccgacgg cgtgcagccc ccaggcctgg tcgacgggcg cgccgctcct
      901 tgtccttcgg acgatgctgg ggctcgaacc gatcggcgac catctggtcg tcgatcccgc
      961 gctccctacg agcatcgggc gccttgagct gctcgacatc ccgggccgat ggggccggat
     1021 ggacgcgttc ggacgcgggc gcgtcgaggt cgggagccac acgtcgcgtg ggcgccggaa
     1081 cacgagatcg cgatcgaaga cccgcggcgg ctgagcgagc caagggactg agccggagga
     1141 ggcaacgaca tgacaggaag cactcgagtg acggaggcaa gacccgccga cgtgctcgtc
     1201 atcttcggca tcacgggcga cctggccaag gtgatgacgt ttcgctccct ctaccggttg
     1261 gagcgtcgcg gtctgctcga ctgcccgatc gtgggcgttg ccgttgacga ctggtccgtc
     1321 gacaacctga aggaacgcgc ccggacgtcg atcgtcggga cgggcgagcc cctggacgac
     1381 gcgatcttcg atcgcctcgc cgctcggttc tcctacgtcg cgggcgactt ctccgacgcg
     1441 gagacgtaca agcacgtcgc cgacacgatc tccgaggccc gggcgccggt cttctacctg
     1501 gagatcccgc cgttcctctt cggcacggtg atcaagggac tccacgaggc gggtctgacc
     1561 gacgctgccc gggtcgtggt cgaaaagccc ttcggccacg atcgcgagtc cgcgaccgtg
     1621 ctcgccgagg agatccacca gtacatcgac gagtcgcagc tgttccggat cgaccactac
     1681 ctcgggaaga tgggcctggt cgagatcctg cacctgcggt tcgcgaactc gatcctcgaa
     1741 ccggtctgga gccgcaactt cctgtcgcac gtgcagatca cgatggccga gtccttcggc
     1801 gtcgaggatc gcggccactt ctacgacccc gtcggcgcgc tccgcgacgt cgtggtgaac
     1861 cacctgatgc aggtggtcgc ggcgtccgcg atggaggcgc cgacgcgcgg agacccgacg
     1921 acgatcaagg acgcgcaggt caacctcttc cgcgccgtgg aggaggccga tccggcgcac
     1981 tacgtccgtg gacagtacga gggatatcgg gcgatcgacg gggtgccgga cgactccacg
     2041 accgagacgt tcgcggcgtt gcggctcgac atcgagagct ggcgctgggc gggtgttccc
     2101 ttcttcatcc gggccggcaa gcgcctgccg atcacccaga cggagctccg cttggtattc
     2161 aagcatccac cgaagctcgg cttcgcggcc ttcgaccgtc ttcccgaggc caaccaactc
     2221 gtggtcaggc tggacccgac gaccgggatc aggctggtgg tcgaggccca ccgagccgac
     2281 gcggagggcg ccgcaccgat cttcctggac gtggacttcg ccgtcgaggg cggggagggt
     2341 ccgacgccct acgaggtact cctgcatgcc gcgatgatcg gagacagcac gcggttcacc
     2401 cgccaggacg gggtcgagga gacctggcgc gtgttccagc cgttgctgga ttcgccaccg
     2461 gccgttcagc cctacgcgca gggttcgtgg gggccctcgg ccaccgatga actcctcgag
     2521 ggcttaggca catggcacga gccatgggtg agccagtgag ccggcgcgcc gaaccgacga
     2581 cgagtggcgt ggcgcagagc gcggcgatgc catcgccctt cccgccgatc gccgactacg
     2641 ccttcctgtc cgactgtcac acgggggccc tgatcgcgcc ggatggcgcg gtggactggc
     2701 tctgcgtccc gcggttcgac tcgccgagca tcttcggcag catgctcgac cgtcaggcgg
     2761 gggcgttccg gttcggtccg ttcgggatca acgtgcccac gcagatcgcg tacgaaccgg
     2821 ggaccaacgt gctcgtgagc acgtggaaga ccccgggcgg ctgggtgatc gtgcgcgacg
     2881 cgctcacgat gggagcgcgc accggcgagg acaaggtgac gccacacact cgaccgccgg
     2941 ccgacgacga cgccgatcac atgctggttc ggacggtgca gtgcctggac gggcaggtcg
     3001 agatcgaact cgtgtgcgag ccgatcttcg actacgggca ggtgccggcg acgtggagcc
     3061 tcggggacgg gaacgacggg gtcgccgacg ccacgggcgg cgatcagatg ttcaggttgc
     3121 ggacggacct gtcgctgggc atcgagggcg accgcgtgcg agcccgtcac cacctgaacg
     3181 ccggggaacg cgcgttctgc gccctgtcgt gggcccacga gctggagacc ccgagcgacg
     3241 tcgacgacgc agaggcccgg atcgcggcga ccgttcggtt ctggcggacc tggctcggcc
     3301 gcgctcggat cccggaccac cgatggcggg aaccgatcca gcgttcggcg ttggcgatca
     3361 agggcctcac gtacatgccg acgggggcga ccgtggcggc gctgacgacg tcgctgcccg
     3421 agacccccgg cggggaacgc aactgggact accggttcac ctgggtccgg gattcgacgt
     3481 tcacccttca ggcactgcac ttcctcaacc tcgattggga ggcggaggag ttcatgcagt
     3541 tcgtagccga tctcgagccg aacgcggacg gcgggctgca gatcatgtac gggatcgacg
     3601 gccgtcgcga cctgagcgaa acgaccctgg acgaactgtc cgggtacgcg ggtgcccatc
     3661 ccgttcggat cgggaacggg gcgttcgatc aacggcagaa cgacgtcttc ggcgccgccc
     3721 tcgactcgat cctcctgcac acccgccgga gccagcggat gccgcggcgc ctgtggccga
     3781 tcgtgcagac ccagtccacg tgcgccaccg agatctggcg ggagcccgat caggggatct
     3841 gggaggcacg cgggaagccg cagcactacg tctcgtccaa gctgatgtgc tgggtggcca
     3901 tggatcgcgc ggccaagctg gccgcgatcg gcggcgacga gggactccgg aacacctggc
     3961 aggggacggc ggacgagatc aaggccgaca tcctcgatca cggcgtcgac gatcgaggtg
     4021 tcctgcgcca gcactacgac accgacgcgc tggacgcgtc ggtcctcctc gcggcgctgt
     4081 tcggcttcct cccgcccgag gacgatcgcc tgcgggcgac ggtgctggcg atcgccgacg
     4141 aactcaccga gaacggctac gtgctccggt acaagacgga cgagaccgac gacggcctgt
     4201 cggggaagga gggaacgttc ctgatctgtt cgttctggct cgtttccgcg ctggcgatca
     4261 tccgagagtg gcaacgcgcc cgggacctga tggagcgcct gcttcgcatc gcctctccct
     4321 tgggcctgtt cgccgaggag ttcgacgtcg agacgggacg gcatctcggc aacttcccgc
     4381 aggcgttctc gcacctggcg ctgatcgagg ccgccggcag aatcatcctg gccgagcgtg
     4441 ttgaggagat ctcatgagcg actacgacgt gatcatcatc ggcaccgggg cgggcggtgg
     4501 gaccctcgcc cgacacctgg ccccgtcggg caagcgcatc ctgctgctcg agcgcggagg
     4561 ctggctggcg cgcgagccgc agaactggag cacggcggac gtcttcatcg acggccggta
     4621 catctcgccc gacacgtggt acgacgccaa gggaaagacc ttccagccgc aggtccacta
     4681 cttcgtgggc ggggcgacca agctctacgg cgcggcgctg tatcgcttgc gggagaagga
     4741 cttcggagag ctcaagtatc ccgatgggat ctcccccgcg tggccgatcg gctactcgga
     4801 catggagccg tactacacgc tggccgagca gcactaccag gtccggggtg cacggggcga
     4861 ggacccgacc gaaccgccct cgagcgcgcc ctaccccttc gcggcccttc cccacgagcc
     4921 caggatccag caactgtccg acgatctcgc ggccgccggt ctccacccgt tccacgcgcc
     4981 gtgcggggtg ctgcgcgaag aggacgcgcc ccagtacagc gtctgcgtcc gatgcggcaa
     5041 ctgcgatggc ttcccgtgtc cgctgcacgc gaaggccgac gccgaggtga tgggcgtgcg
     5101 cccggccctg gagtcgccga acgtcaccct gttcaccgac gccccggcgg tgcgcctgaa
     5161 cacgaacccc gagggcacgt ccgtaaccga ggtcgtgatc gaactcgacg gctccaccga
     5221 gaccgtgacc ggcgacatcg tcgtggtctc gtgcggtgcg gccaactcgg ccaagctgtt
     5281 gctggcgtcg gcgaacgacc agcacccgaa gggtctcgcc aacggctcgg accaggtggg
     5341 ccgcaactac atgttccact acagccaggc ggtgttggcc gtgtccaagg aacccaaccc
     5401 caccgtgttc cagaagaccc tcggggtgaa cgacttctac ttcggggacg aagagtacga
     5461 gtacccgatg ggcaacatcc agatggtcgg caagtcacag gccgagatgt acaagggcga
     5521 gaaaccgatc gagaccaagc tcgcgccgac gttcgcgctc agggacatcg ccacacatgc
     5581 cgtcgacttc tggctgtcga ccgaggacct tccgctcccg gagaatcgcg tgacgttgga
     5641 gaaggacggc aacgtccggc tcgcgtacga acccagcaac gaggagtccg ggaagcgcct
     5701 gtaccacaag ctgaagtcga tgctgggtca cctcggtctg cacccggacc acctgatccc
     5761 acggaacgcc tatctgaaga cgaccatccc cgtggccggc gtcgctcatc aggcgggcac
     5821 gtgccggatg gggatcgatc ccgcgatgtc ggtcgtcgat gcgaactgca aggcgcacga
     5881 gcttgacaac ctgtacatcg tcgacacgag cgtcttcccg agcatcggcg cggtgaaccc
     5941 cgccctgacc gcgatggcga acgcgctccg ggtgggcgac catctgctcg aacgtttcta
     6001 ggtcggccct atccctggcg cggcgccatc ccccacaccc ccgaccacct agcgcccggc
     6061 tcgagccgga tcacgtcgac gccggacgcg aacgcgttgg gtgggcacgt catcggttcg
     6121 atcgcgacgg catgccgccg gcgcgaggcc ggctcgaggg tgtctcccgt gtagacctgg
     6181 aggtagccga acgcctcctc cgcccagacc tcgacgccgc gtccgccctc ggccgactcg
     6241 agccgggcgc gcgaccggcc gtcggacccg cgagcgaggc cggtgaagca agtgtcgagc
     6301 cgtgtggccc cgacggaacg accgacgccg aagtcgaact ccgtgccggc gaggtccacc
     6361 tcgccgatcg gcagggctcg gtcgtccgtg gtgagcctgc gcgaggcaag gatcgtgagc
     6421 cgcacgtcgt cgaccgggat ccccagcgtc gggtaggggt ggaacccgat cccgaacggc
     6481 gcagcctcgg cggaggcgtt catcgccaac gcgacgacct cgagcccgtc ccccacgagc
     6541 cgatactgga ggcccagctc gagccgccat gggtacgcgg gctgggggtg caggacgcac
     6601 tccagcgcga catagtcgtc gcgcttggag gccagctgcc aggggagcca acgcaccagg
     6661 ccgtggatcg cgttgccgag ctcgggctcg tcgatcgcgg ccgccccacg gcgtccgtcg
     6721 aactcgtatc gcccgccgtc gagacggttc ggccacggcg ccagcacctg gccgcgtccg
     6781 gccgatgagg gctcgtcgac gccgaacccg tccaggacat cgagaccgcc cgccgagtac
     6841 gcccggagcg tcgcgccgac ctcggccacg acgacgacct gatcgccgtg gcggacctcg
     6901 tgctgttcgc ccgaaggcgg gtgaggctgc acgtccttcc cgctctcgct cgacaccgtc
     6961 agtagctgct gggcgtgagc ttgaccttcc cgctgaacag gtactgcacc cccgccgtgt
     7021 acaggagcac gaacgggatc ccgacgatcg ccaccaccag catcaccgtg agcgtgtagt
     7081 gctcggcgga ggcgttcgag accgtcatgc tgaaggccgg gttcgtcgac gacttcagca
     7141 tattgggata caggccggcc gagatcgtcg cgagcgagag gaagagcacc gccgagctgc
     7201 cgaagaacgc ggcgaagtcc cgcccgcgcc gaaccatcac gatcatcagc aggtaggcga
     7261 tcagcgcggc cgccggaaac acgatcgtcc agacgtcgtc cgtgaacgta tccgtcacgc
     7321 cgtagtcccc ggccgcgatc cacacggccg agatcgccgc cgtcagggtg aacagggcga
     7381 tgaaccgggg gacccatcgt cgcacgcgcg cctggagatc gcc
//
LOCUS       DATBRK010000391         5786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1088280, whole genome shotgun sequence.
ACCESSION   DATBRK010000391 DATBRK010000000
VERSION     DATBRK010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088280"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VIH70_11550"
     CDS             complement(<1..399)
                     /locus_tag="VIH70_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY8117040.1"
                     /translation="MSAETYVPSDPELRSILGDARTIAVVGLSSNPERASYEVAEYLQ
                     SKGYRIVPVNPNETEVLGERAYPTLADIPSEIEIDVVDVFRRAEQTPAIAEEAVARGA
                     KVLWLQEGIANDDARRIGEAGGLSVVMGVCM"
     gene            complement(396..869)
                     /locus_tag="VIH70_11555"
     CDS             complement(396..869)
                     /locus_tag="VIH70_11555"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY8117041.1"
                     /translation="MLLPLVEGDRVVFTRRTEHLSRHPGEISFPGGLEHEEDADLIAT
                     ALRETEEELGLPRERVEVLGALEPVHTFVSAILIVPFVGVVSGTPAFAPSEDEIAEVL
                     SYPVGDLSAAETTVEWPRDGHVYRGFAYPMSDGNTIWGATAKILNEMIELLRGRP"
     gene            968..2158
                     /locus_tag="VIH70_11560"
     CDS             968..2158
                     /locus_tag="VIH70_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY8117042.1"
                     /translation="MVEEAVVSAVVDQVDPEEVVRFARRLVSTPSENPGDTEEAVAAV
                     AVEILEGLGDSPEIVRGEEGRPSVVARFGNGERPALAWNGHLDVVPAGDPSTWRHPPF
                     GAELVDGSLVGRGSADMKGGIASAFGAVSAIRRSGVELAGRLDLHLAADEELAGLHGT
                     TVLLERGLLDQDAAIVGEPTELNLALAERGGAWITATARGTAAHGSTPRLGVSAITSM
                     ARFLLRIEEVLPDIEHPLVGRPTVNAAMIQGGSAPNVVADRCVVEIDRRIIPGETEPG
                     KVLEPFDRLAEAIRSEHPEADLSFAIRLWTDAAETTGDPTIADLCRAAVREETGRPPL
                     DTGMTGITDARFYLNDRSIPTIILGPGSLGVAHTANESVEIAQLVAAARVYARVFTRF
                     LDAS"
     gene            2196..2540
                     /locus_tag="VIH70_11565"
     CDS             2196..2540
                     /locus_tag="VIH70_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY8117043.1"
                     /translation="MEQTATVITVTDRAAGKIRQLAAKEGRTEPVLRVRVVAGGCSGF
                     SYELSFADAPEGDDHVVPGADGVRVLVDRRSAPIVEGSTLEFNDALLGGGLKMLNPRA
                     KHECACGDSFSV"
     gene            complement(2564..2956)
                     /locus_tag="VIH70_11570"
     CDS             complement(2564..2956)
                     /locus_tag="VIH70_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8117044.1"
                     /translation="MTKARWVRFVAGLFVLSLVVAACSSDDEAGGTTGTTGGATGATQ
                     TGSGGGGTTVTISGFAFHPDTITVSGPTEVTITNEDSTTHTFTLDDGSVDETVEPGAT
                     ETVTVDVSASTGFHCNIHPQMTGQIEVS"
     gene            3065..4198
                     /locus_tag="VIH70_11575"
     CDS             3065..4198
                     /locus_tag="VIH70_11575"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEY8117045.1"
                     /translation="MPSSADKTQWRDLYNEVVLTGLCTGCTACIVACPFHVLGYEDDE
                     PVQLQEDGPDACVHGDRGCSLCTLACPRFREWESEIDQLLFGQTRKPEDVIGQYQDIV
                     LARATMPEALMQGQDGGVVSALLIWGLRTGEIDGALTSKLSDDREWDAEPTVVTDAEG
                     VLATAGSRYTYSANPLALIKAAELGLSKVALVGMSCQASVTGSMTARRVNKWAKKINW
                     TFGLLCSKSFTYDGLMVEIAQKRLGLELDDLVRVNVKGKLLFYTKDGEEVTYSLKQAH
                     EFTRPGCLRCPDFAAEHADISFGGLGQSDGWTLTIIRTDKGRDLWDRAAADGVVEWRP
                     GSEDPAAISLMSKLAGKSRARWGETPWGAPGEMPEPEPASESG"
     gene            complement(4258..4587)
                     /gene="fdxA"
                     /locus_tag="VIH70_11580"
     CDS             complement(4258..4587)
                     /gene="fdxA"
                     /locus_tag="VIH70_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018159792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY8117046.1"
                     /translation="MTYTICEPCIDIKDKGCVDECPVDCIYEGPRMLYIHPDECVDCG
                     ACEPVCPVTAIFYEDDVPAEWKQFTPINAEWFNDNVSGVGSPGGAAQVGPSNVDHPVV
                     AAWPTVG"
     gene            complement(4705..5643)
                     /locus_tag="VIH70_11585"
     CDS             complement(4705..5643)
                     /locus_tag="VIH70_11585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8117047.1"
                     /translation="MAARRQPSGTGFALRDALPWTEIARIVREVEPAGYTAVFLPEIT
                     GRDALVTLGMLAGETRRLLLGTGVLPMRSRTALLTAMGAATVHERSGGRLILGLGTGE
                     VERGALDELRETVRRVRALLRGETLEEGESVTLSLRPGREVPIWVSALGPKAMRLAGE
                     IADGVILNWCPPERVSFARARIAEGAEAAERDPVSIAVAVYVRAWVGGDEAEAISALR
                     AMAGRYASYRAYRRQFEQVGLGPQAAVAGQAHRAGRSEDVPEVLVRSVCAVGDGARDR
                     LDAFRQAGADLPIVYPVATADPAASIESTLRALAPA"
BASE COUNT          837 a         2090 c         2017 g          842 t
ORIGIN      
        1 catgcacaca cccatgacga ccgacaggcc gccggcctcg ccgatccggc gcgcgtcgtc
       61 gttggcgatc ccctcctgca gccacagcac cttcgcgccg cgcgcgaccg cctcctcggc
      121 gatcgccggc gtttgctcgg cgcgacggaa cacgtcgacg acgtcgatct cgatctcgct
      181 cgggatgtcg gccagcgtcg ggtaggcgcg ctcgccgagc acctcggtct cgttcgggtt
      241 cacgggtacg atccggtacc ccttcgactg caggtactcc gcgacctcgt aggaggcgcg
      301 ctcggggttc gacgacaggc ccacgacggc gatcgtgcgc gcgtcgccca ggatcgaacg
      361 gagctctggg tcgctcggca cgtaggtctc ggcgctcacg gccgccctcg aaggagctcg
      421 atcatctcgt tcaggatctt cgcggtcgcg ccccagatcg tgttgccgtc gctcatcggg
      481 tacgcgaacc cacggtacac gtgtccgtcc ctcggccact cgaccgtggt ctctgccgcc
      541 gagagatcgc ccacaggata ggagagcacc tcggcgatct cgtcctcgct cggcgcgaac
      601 gccggcgtcc ccgacaccac gccgacgaac ggcacgatca ggatggccga gacgaacgtg
      661 tgcacgggct cgagcgcccc gagcacctcg acgcgttcgc gcggcaggcc gagctcctcc
      721 tcggtctcgc gcagcgccgt ggcgatcagg tcggcgtcct cctcgtgctc gagcccccct
      781 gggaacgaga tctcgccggg gtggcgcgac aggtgctcgg ttcgccgggt gaagaccacc
      841 cggtccccct cgaccagcgg gagcagcacc gccgcgagcc gatcacccgg agccgggacc
      901 gcgtggggtg aacgtcggag gctgccttcc aggcgcgtgc gaacgtcgct ccggtacatt
      961 cgccgccatg gtagaggagg ccgtcgtttc ggccgtggtc gaccaggtcg acccggagga
     1021 ggtcgtccgc ttcgcgcgcc ggctcgtctc gaccccgagc gagaaccccg gggacaccga
     1081 ggaggcggtg gccgcggtcg cggtggagat cctcgaaggg ctcggcgact cgcccgagat
     1141 cgtgcgcggc gaggaggggc gtccgagcgt ggtcgcccgg ttcgggaacg gcgagcggcc
     1201 cgcgctcgcg tggaacggtc acctcgacgt cgtccccgcc ggcgacccct cgacgtggcg
     1261 gcatccgccg ttcggcgccg agctggtcga cggctcgctg gtcggacgag ggtccgccga
     1321 catgaagggc ggcatcgcga gcgccttcgg cgccgtctcg gcgatccgtc gttcgggcgt
     1381 cgagctcgcc ggcaggctgg acctgcacct cgcggccgac gaggagctcg cgggccttca
     1441 cgggacgacg gtcctgctcg aacgcggcct cctcgatcag gacgcggcca tcgtcggcga
     1501 gccgaccgag ctgaacctcg cactcgcgga gcgcggcggt gcctggatca ccgccaccgc
     1561 ccggggaacg gcggcccacg ggtccacccc gcggctcggc gtgagcgcta tcacctcgat
     1621 ggcgcgcttc ctgctccgga tcgaggaggt gctgcccgac atcgaacatc cgctcgtggg
     1681 acggccgacg gtgaatgcgg cgatgatcca gggcgggagc gcacccaacg tcgtcgcgga
     1741 ccgttgcgtg gtcgagatcg atcgccggat catccccggc gagaccgagc ccgggaaggt
     1801 gctcgaaccg ttcgacaggc tcgccgaagc gatccgcagc gagcaccccg aggccgatct
     1861 gtcgttcgcg atccggctgt ggaccgacgc ggccgagacg accggcgacc cgacgatcgc
     1921 cgacctctgc cgcgccgcgg tccgggagga gaccggtcgc ccgccgctcg acaccgggat
     1981 gaccggcatc acggacgccc gcttctatct gaacgaccgg agcatcccga cgatcatcct
     2041 cggtcccggc tccctgggcg tcgcccacac ggcgaacgag tcggtggaga tcgcccagct
     2101 ggtcgccgcg gcccgcgtct acgcccgggt gttcacgcga ttcctcgatg cttcgtgacg
     2161 gatcggtgac gcaggcacgg ggtatcctgg gagccatgga gcagaccgcc acggtcatca
     2221 cggtgacgga tcgggccgcc ggcaagatcc ggcagctcgc cgccaaggag gggcggaccg
     2281 agccggtcct ccgcgtccgt gtggtcgccg gcgggtgctc cggcttctcc tacgagctgt
     2341 ccttcgccga cgcccccgag ggggacgacc acgtggtccc aggggcggac ggcgttcggg
     2401 tcctggtcga ccgccggagc gcgccgatcg tcgagggctc gaccctcgag ttcaacgacg
     2461 ccctcctcgg tggcggcctc aagatgctga acccgcgcgc gaagcacgag tgcgcctgcg
     2521 gcgactcgtt ctccgtctga gctccgactg gcgggatccc gcgctacgag acctcgatct
     2581 gccccgtcat ctgcgggtgg atgttgcagt ggaagcccgt cgacgcggac acgtcgaccg
     2641 tgacggtctc cgttgcgccc ggctcgaccg tctcgtccac gctgccgtcg tcgagcgtga
     2701 acgtgtgggt ggtgctgtcc tcgttcgtga tcgtcacctc ggtcgggccg gaaaccgtga
     2761 tcgtgtccgg gtggaacgcg aacccggaga tcgtgacggt cgtcccgccg ccgccggaac
     2821 cggtctgcgt ggcgccggtg gcgcccccgg tcgtgccggt cgtgccgccg gcctcgtcgt
     2881 cactgctaca cgcggccacg acgagcgaga gcacgaagag cccggccacg aaccggaccc
     2941 acctcgcctt cgtcatcctc cacctccgtc gtcggacgtc cggccaccct agcccacgag
     3001 ccccgaaaac gccggggctc gttgccgccg gggtttcgga cgtctgctcc tatacttcgc
     3061 cctgatgccg agcagcgccg acaagacgca gtggcgcgac ctctacaacg aggtcgtgct
     3121 cacggggctg tgcaccgggt gcaccgcgtg catcgtggcg tgtccgttcc acgtcctcgg
     3181 ctacgaggac gacgaacccg tccagctgca ggaggacggt ccggacgcgt gcgtccacgg
     3241 cgaccgaggg tgctcgctct gcacccttgc ctgccctcgc ttccgcgagt gggagtccga
     3301 gatcgaccag ctcctgttcg gccagacgcg caagccggag gacgtgatcg gccagtacca
     3361 ggacatcgtc cttgcgcgcg ccacgatgcc cgaggcgctc atgcagggcc aggacggcgg
     3421 ggtcgtctcg gcgctactga tctggggcct gcgcacgggc gagatcgacg gagccctcac
     3481 gtccaagctc tcggacgatc gcgagtggga cgccgagccc acggtcgtca cggacgcgga
     3541 gggggtgctc gcgacggccg ggtcccgata cacctactcg gcgaacccgc tcgcgctcat
     3601 caaggccgcc gagctgggcc tgtcgaaggt ggcgctcgtg gggatgtcgt gccaggccag
     3661 cgtgacgggt tcgatgacgg ccaggcgcgt caacaagtgg gcgaagaaga tcaactggac
     3721 gttcgggctc ctgtgctcga agagcttcac ctacgacggc ctgatggtcg agatcgccca
     3781 gaaacggctg gggctcgagc tcgacgatct cgtccgggtg aacgtgaagg gcaagctgct
     3841 cttctacacg aaggatggcg aggaggtcac gtactcgctg aagcaggcgc acgagttcac
     3901 gcgcccggga tgccttcggt gcccggactt cgccgcggag cacgccgaca tctcgttcgg
     3961 cgggctcggg cagagcgacg gctggacgct gaccatcatc cgtacggaca agggtcgcga
     4021 tctgtgggat cgcgcggcgg cggatggggt ggtggaatgg cggccggggt cggaggaccc
     4081 cgccgcgatc tccctgatgt cgaagctggc cggcaagtcg cgggctcgtt ggggcgagac
     4141 cccctggggt gccccgggcg agatgcccga acccgaaccc gcctccgaga gcggctaggc
     4201 gcgccgatcc ggcggcatct cggctaggag cggccgacgc gtgcgccccg agatccgtca
     4261 gccgacggtg ggccaggcgg cgaccaccgg atggtccacg ttgctggggc cgacctgggc
     4321 cgctccgccg ggggacccga cgccgctgac gttgtcgttg aaccactcgg cgttgatcgg
     4381 ggtgaactgc ttccattccg ccggcacgtc gtcctcatag aagatcgcgg tcaccgggca
     4441 caccggctcg cacgcgccgc agtccacgca ctcgtcggga tggatgtaaa gcatccgggg
     4501 tccctcgtag atgcaatcga ccgggcactc gtccacgcag cccttgtcct tgatgtcgat
     4561 gcacggctcg cagatcgtgt acgtcatggc agcgcttccc ccttcgctga gccttcgccg
     4621 ggttttcccc ttttccacgg tgccgacctt gtaaaagcta gtctacggct cacgggctcg
     4681 tgcgcaagtc gggcgtggga tccctcaggc cggggcgagc gcgcggagcg tcgactcgat
     4741 cgaggcggcg gggtcggcgg tcgcgaccgg gtagacgatc ggcaggtccg cccccgcctg
     4801 cctgaacgcg tcgagacgat cccgagcccc gtcgcccacg gcgcacaccg accggaccag
     4861 cacctcgggc acgtcctcgg atctcccggc ccggtgcgcc tgtccggcga ccgccgcctg
     4921 gggcccgaga cccacctgct cgaactgacg ccggtaggcc cggtaggagg cgtaccgacc
     4981 cgccatcgcg cgcaaggcgg agatcgcctc ggcctcgtcg ccccccaccc acgcccgcac
     5041 gtacaccgcg acggcgatgg acaccgggtc tcgctccgcc gcctcggcac cctcggcgat
     5101 gcgcgcgcgg gcgaacgaaa cccgctcggg gggacaccag ttgaggatga cgccgtcggc
     5161 gatctcgccc gcgagccgca tcgccttggg cccgagcgcg gagacccaga tcgggacctc
     5221 gcgccccggc cgcagcgaga gggtcaccga ctcaccctcc tcgagcgtct cgccccggag
     5281 cagggcacgg acccgtcgca ccgtctcacg gagctcgtcg agcgcacccc gttcgacctc
     5341 ccccgttccc agaccgagga tcagccggcc ccccgagcgc tcatgcaccg tggccgcgcc
     5401 catcgcggtg agcagcgcgg tccgggagcg catcgggagc acacccgtgc ccaggagcag
     5461 gcggcgcgtc tccccggcga gcatcccgag cgtgaccagg gcgtcgcggc ccgtgatctc
     5521 cgggaggaac accgccgtgt atccggcggg ttcgacctcg cgcacgatcc gtgcgatctc
     5581 cgtccagggc aaggcgtcgc ggagcgcgaa ccccgttccg ctcggttgtc gacgcgccgc
     5641 cacggctggc atcgtcgcac gcatggaacc cgtgcgtttc gggacggagg acggcgtgtc
     5701 gctcgagggg gagatccggc ggcccgagac gccgccgtcc ggctcggccg tgatctgcca
     5761 cgcgcacccc cgtcacggcg ggagca
//
LOCUS       DATBRK010000392         6210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2535535, whole genome shotgun sequence.
ACCESSION   DATBRK010000392 DATBRK010000000
VERSION     DATBRK010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535535"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VIH70_11590"
     CDS             <1..405
                     /locus_tag="VIH70_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8117048.1"
                     /translation="FSEIIRRGSEAAQGLPADIVYAGFSLGVMPAQSLTQTRQGARGA
                     ILFHGALPTSEFEGPWPEGVPLQIHMMDEDPWAGEDRPAAEALVEQIEGAELFIYPGS
                     GHLFADPSSDDYDEQAAGLLKERTLAFLERVG"
     gene            complement(483..1268)
                     /gene="panB"
                     /locus_tag="VIH70_11595"
     CDS             complement(483..1268)
                     /gene="panB"
                     /locus_tag="VIH70_11595"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY8117049.1"
                     /translation="MTITIRDLQAWKAGGKRFSMLTAYDYPTAKIFDEAGIPVLLVGD
                     SVGNTILGYENTLPVTMDEMLHHTRAVARGVTNALVVGDMPFLSYQVGVEEGVRNAGR
                     FLKEAGAGAVKIEGARVELVHTLVELGIPVMAHVGLTPQSVHAIGGYRVQGRGEEAAR
                     VTDQALALEKAGAFAIVLEAMPSELGAELTTTLQIPTIGIGAGPGTDAQVLVSPDLLG
                     LSERVPRFARAYVDLRGEIAKAATEFAADVAAGAFPDEEHSYT"
     gene            1432..1758
                     /gene="rpsF"
                     /locus_tag="VIH70_11600"
     CDS             1432..1758
                     /gene="rpsF"
                     /locus_tag="VIH70_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY8117050.1"
                     /translation="MGAVQTRGGGELRQYEGMLILPAEADESVVSGALDRITRVVGEA
                     GGQVGNVDRWGRRRLAFEIERQAEGYYVVVEFTADPGVLTDLERTLRLADEVLRFKIV
                     VRGEAA"
     gene            1829..2272
                     /gene="ssb"
                     /locus_tag="VIH70_11605"
     CDS             1829..2272
                     /gene="ssb"
                     /locus_tag="VIH70_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8117051.1"
                     /translation="MASENQVTIVGNLTDDPELRYTPNGAAVVKFRVAVNRRYKDESG
                     NWKDGETSYFSVNAWRSLAENSAETLTRGTRVVVTGRLQMRSWETQEGDKRTVVEIEA
                     DELGPSLRWATAKVERQGRSGGSSDWTPATVGAPMADEETPLDAT"
     gene            2262..2534
                     /gene="rpsR"
                     /locus_tag="VIH70_11610"
     CDS             2262..2534
                     /gene="rpsR"
                     /locus_tag="VIH70_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY8117052.1"
                     /translation="MPPKTKKRPAKREKDDKGWQKKQKRKICIFCKDRIEYVDYKDTS
                     TLRRFVSERGKIRSRRVTGNCSQHQRDVATAVKNAREMALLPYSTR"
     gene            2531..2983
                     /gene="rplI"
                     /locus_tag="VIH70_11615"
     CDS             2531..2983
                     /gene="rplI"
                     /locus_tag="VIH70_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY8117053.1"
                     /translation="MKIILQKPVDKLGVPGDIVDVADGYARNYLMPRGLAVKATKGGV
                     KHVDSLRRAHSTRVDAAKAEAEQVAQRLTASPLTVAAHAGEEGRLFGSVTPSDVAEAI
                     QAQTGVRVDRHDVTMDEPIRSVGVHEVKVHLFAEVDPVISVEVTASEE"
     gene            complement(2994..3278)
                     /locus_tag="VIH70_11620"
     CDS             complement(2994..3278)
                     /locus_tag="VIH70_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117054.1"
                     /translation="MSATAGRIRAAGTLRRAEMPRAEIRAVLAADDPEIVRRYLELHR
                     ERLEERMAAHRRELEILACSLTEHLEARGRRAERRRRGRRPDLGPTAEVG"
     gene            3483..4361
                     /locus_tag="VIH70_11625"
     CDS             3483..4361
                     /locus_tag="VIH70_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEY8117055.1"
                     /translation="MSDPYEEFRPLMFSIAYRMLGSASEAEDIVQEAFLRFHRESTAG
                     TVIESPKAYLSTVTTRLAIDQLRSARVRRERYVGTWLPEPLVTDTESDVARHAEAADS
                     LSMAFLLLLESLSPVERAVFLLREVFDYGYDEIADVVGKSEDNCRQIAVRARRRVESH
                     KPRFEASRERREELARRFFAAVTEGDTDGLLALLAADVVAYGDGGGKAPAFPKPVYGR
                     DHVARLLFRAAEQGLKRGVIGARYVEVTGQPGAIFVDPDGRPLAVVTLDIADDLVQTV
                     RAVSNPEKLRHLTTSP"
     gene            4841..6190
                     /gene="dnaB"
                     /locus_tag="VIH70_11630"
     CDS             4841..6190
                     /gene="dnaB"
                     /locus_tag="VIH70_11630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY8117056.1"
                     /translation="MRAADDVRRIRPERVPPHNLEAEESVLGSMMLSPDAIADVIEIV
                     RPVDFYRTPNAKIFDTLREIYGRGDPVDAITAVEELKRRGTLEEVGGHLAISDLVDQV
                     PTPSSAASYARIVAETSLLRRLIGAAADIMDMAYSAPEEPEHVADVAEQRIYEVARHG
                     EKEQVASLHELVDEAMTDLERIQNRESAFAGVPTGFRDVDTLLSGMQSGNLIVIAARP
                     GVGKSSFVTNVARNVAVDAGLPVAMFSLEMSRWEIGMRLLCGEARVPWDSIRNKRVGA
                     EDWSRIAQAADSLHEAPLFIVDSGNVTLVDIRAKARRLSARRAGLGMIIVDYLQLMSH
                     TRRVDNRQQEIAEISRGLKMLAKELEIPVIAVSQLNRDPERRQDKRPQLSDLRESGAI
                     EQDADVVMFIHREDADPAKKGLADLIVAKHRNGPTDTVPLTFLPHLTQFRNHATAVT"
BASE COUNT          985 a         2141 c         2179 g          905 t
ORIGIN      
        1 ttctcggaga tcatccgccg cggttcggag gcggcccaag ggctcccggc cgacatcgtc
       61 tacgcgggct tctcgctcgg ggtgatgccg gcgcagtcgc tgacgcagac tcgccagggt
      121 gcgcgggggg cgatcctgtt ccacggggcg ttgccgacct ccgagttcga gggcccctgg
      181 cccgagggcg tgccgctcca gatccacatg atggatgagg acccatgggc gggcgaggat
      241 cgacccgcgg ccgaggcctt ggtcgagcag atcgagggcg cggagctgtt catctatccg
      301 ggttcgggtc acctgttcgc cgacccgagc tcggacgact acgacgagca ggccgccggg
      361 ctattgaagg agcgcacgct cgccttcctg gagcgggtcg gctagttcga gatcgcgcgt
      421 tcgcctcgct ccgagcctct gttggccccg ccggacgagc tccggcgtga gcgcgagatc
      481 cgctacgtgt aggagtgctc ctcgtcgggg aacgccccgg cggcgacgtc ggcggcgaac
      541 tcggtcgcgg ccttcgcgat ctcgccgcgc aggtcgacgt aggcgcgcgc gaacctcggc
      601 acgcgttccg agagcccgag caggtcgggg gacacgagca cctgcgcgtc ggtccccggt
      661 cccgcgccga tgccgatcgt cgggatctgc agggtcgtgg tgagctccgc gccgagctcg
      721 gacggcatgg cctccagcac gatcgcgaac gcgcccgcct tctcgagcgc gagcgcctga
      781 tcggtcaccc gcgcggcttc ctcgccgcgg ccctgcacgc ggtacccgcc aatcgcgtgc
      841 accgactgcg gcgtcaggcc gacgtgcgcc atcacgggga tcccgagctc cacgagcgtg
      901 tgcaccagct cgacccgcgc gccctcgatc ttgacggcgc cggcgcccgc ctccttcagg
      961 aagcggccgg cgttgcggac gccctcctcg acgccgacct ggtacgacag gaacggcatg
     1021 tcgcccacta ccagcgcgtt ggtgacgccg cgcgcgaccg cgcgcgtgtg gtgcagcatc
     1081 tcgtccatgg tcacgggcag cgtgttctcg tagccgagga tcgtgttgcc gacggagtcg
     1141 ccgacgagca acacggggat cccggcctcg tcgaagatct tggcggtggg gtagtcgtag
     1201 gccgtgagca tcgagaagcg cttgcccccg gccttccacg cttgcaggtc acggatcgtg
     1261 atggtcatcg ctcgcgcctc cttcgtgagt gcacggcagt cgctccgagc agtctacccg
     1321 ggggcgccgg cgggacagcg ggccgcgtcc gtgcgtgtca ccggcgggtg ctagggtcgg
     1381 ccgaccagcg agccgctaga ctgggcgcca gccacacatc ctgctccccc ggtgggggcc
     1441 gtccagacca gaggaggtgg agaactgagg cagtacgaag gcatgctcat cctgcccgcg
     1501 gaggccgacg agtcggtcgt gtccggggcg ctggaccgca tcacccgcgt cgtcggcgag
     1561 gcgggtggcc aggtcggcaa cgtcgatcgc tggggccgcc ggcgtctggc attcgagatc
     1621 gaacgacagg ccgagggcta ctacgtcgtg gtcgagttca cggcggatcc cggggtcctg
     1681 accgatctcg aacggacgct gcggctggcc gacgaggtcc tgcggttcaa gatcgtcgtt
     1741 cgcggcgagg ccgcgtaacg aagagcgact tccccctccc cggtacgcca tccgacgggg
     1801 agcggtcacg gaacggaagg tgatggagat ggcgagcgag aaccaggtca ccatcgtggg
     1861 aaacctcacg gacgatcccg agctgcgcta cacgccgaac ggggccgccg tcgtgaagtt
     1921 ccgcgtggcg gtcaaccgcc gctacaagga cgagtcgggc aactggaagg acggcgagac
     1981 gtcgtacttc tcggtcaacg cctggcggag cctggccgag aacagtgccg agaccctcac
     2041 ccgaggcacg cgcgtcgtcg tgaccggccg gctgcagatg cgctcctggg agacccagga
     2101 aggcgacaag cgcacggtgg tcgagatcga ggccgacgag ctcggcccga gcctgcgttg
     2161 ggcgaccgcg aaggtcgagc gccagggtcg ctcgggaggt tcgagcgact ggactcccgc
     2221 cacggtcggc gctcccatgg cagacgagga gacaccgctg gatgccacct aagacgaaga
     2281 agcgacccgc caagcgggag aaggacgaca agggctggca gaagaagcag aagcgcaaga
     2341 tctgcatctt ctgcaaggac cggatcgagt acgtcgacta caaggacacc tcgacgctgc
     2401 ggcggttcgt gtccgaacgc ggcaagatcc gctcgcgccg ggtgacgggc aactgctcgc
     2461 agcaccagcg cgacgtcgct accgcggtca agaacgcccg cgagatggcc ctgctgccct
     2521 attcgacacg atgaagatca tcctgcaaaa gcccgtcgac aagctcggcg tcccggggga
     2581 catcgtggac gtggccgatg gctacgcgcg caactacctg atgccccgcg ggctcgccgt
     2641 gaaggcgacc aagggcggcg tgaagcacgt cgacagcctc aggcgcgctc acagcacgcg
     2701 cgtcgacgcg gccaaggccg aggccgaaca ggtcgcccag cgcctcacgg cttcgcccct
     2761 cacggtcgcg gcgcacgccg gggaagaggg ccggctgttc ggcagcgtca cgccgagcga
     2821 tgtcgccgag gcgatccagg cgcagaccgg cgttcgggtc gaccggcacg acgtcacgat
     2881 ggacgagccc atccgctcgg tgggagtgca cgaggtcaag gtgcaccttt tcgccgaggt
     2941 cgaccccgtg atctcggtcg aggtcacggc gtccgaggag taggaccgcg gagtcacccg
     3001 acctcggccg tggggccgag gtcgggccgc cgcccccgtc tgcgccgctc ggcgcgtcgg
     3061 ccccgcgcct ccaggtgttc ggtcagggag cacgccagga tctcgagctc ccgacgatgg
     3121 gccgccatcc gctcctcgag gcgctctcgg tgcagctcga ggtatcggcg gacgatctcg
     3181 gggtcgtcgg ccgcgagtac ggcgcggatc tcggccctcg gcatctcggc gcgacggagc
     3241 gtgcccgcgg ctcggatccg gccggcggtg gcgctcatcg gccgtctcgg cgcgccggcc
     3301 ggagcatcgg ggcaccgacg acgaacatgg gtctccatcc gcgactcctt tgcgcgtgtt
     3361 cacccatcgg gacgagacga cccggccgcg tgtgacaccg gcaggaggtg gagggatgtc
     3421 acaacggggc cggccgtctc gtctcctgag atgatgacgt ctgggcacct ggggaggcac
     3481 gtgtgagtga cccgtacgag gagttccggc ccctgatgtt ctcgatcgcc taccggatgc
     3541 ttgggagcgc gagcgaggcg gaagacatcg tgcaggaggc gttcctgcgg ttccaccgcg
     3601 aatcgaccgc cggcaccgtg atcgagtcac cgaaggccta tctgtccacc gtcacgacac
     3661 ggctcgcgat cgaccagctg cggtccgctc gtgtgcgccg ggagcgctac gtcggcacgt
     3721 ggctgcccga accgctcgtg accgacaccg agtccgatgt cgctcggcac gccgaggccg
     3781 ccgactcgct ctcgatggcg ttcctcctcc tcctcgagag cctctccccg gtcgagcgag
     3841 cggtcttcct gctccgcgag gtgttcgact acggctacga cgagatcgcc gacgtggtag
     3901 gcaagagcga ggacaactgc cgacagatcg ccgtccgcgc ccgacgccgg gtcgagtcgc
     3961 acaagccgcg gttcgaggcc tcacgcgagc gaagagagga gctcgcgcgc cggttcttcg
     4021 ccgccgtcac cgagggcgac accgatggcc tcctcgcgtt gctggcggcc gacgtggtcg
     4081 cgtacggcga cggtggcggc aaggcgcccg ccttcccgaa gcccgtctac ggccgcgacc
     4141 acgtcgcccg tctgctgttc cgagccgcgg agcaaggcct gaagcggggc gtgatcgggg
     4201 cccgatacgt cgaggtcacc gggcagcccg gggcgatctt cgtcgatccc gacggtcggc
     4261 cgttggcggt ggtcacgctc gacatcgcgg acgatctggt ccagacggtc cgggccgtct
     4321 ccaacccgga gaagcttcgc cacctgacca cgtccccgtg atcagctcgg acggtgaggc
     4381 ggtcgcggcg cggggatcga ggagtcgaac gaggcgcaat cgctcccggc gaacctggga
     4441 gagctcgagc cgacccgctt gttgatctcg aagatcctcc ggcacgaccc tgccacgcgg
     4501 gcgagatcaa ccacttccga ggcttgctcg tcgaggacgg ccgctggcgg ttgggctgac
     4561 agatcccgtc gcggtccccg acctcggccc gcccctggac gccggccacc ggccggagga
     4621 gaatcacacg cgtgtgatcc cgatgtcggc ggggaacgat acacacctat ccacagggtt
     4681 ttccccctgg ggaagcgctg gtggagggca cagtgacaag gaatcttcag gcacgaccag
     4741 cggatcgggt ttccccacgg gttttccgct gggaggtggg gctccacggg gctagcgttg
     4801 cccgccggtt ccatcggcgt tcgagggggg cgcagggtcg gtgagggcag cggacgacgt
     4861 ccggcggatc agaccggagc gggtgccccc gcacaacctc gaggccgagg agtccgtcct
     4921 cggctccatg atgctgtcgc ccgacgcgat cgccgacgtc atcgagatcg tccgccccgt
     4981 cgacttctac cggaccccga acgccaagat cttcgacacc ctccgcgaga tctacggccg
     5041 gggcgacccg gtcgacgcga tcaccgcggt ggaggagctg aagcgccggg ggacgctcga
     5101 ggaagtgggc ggccacctcg cgatctccga tctcgtcgac caggtcccca cgccctcgtc
     5161 cgccgcgtcc tacgcccgga tcgtcgccga gacctcgctg ctccgccggc tgatcggcgc
     5221 ggccgccgac atcatggaca tggcctactc ggcccccgag gagcccgagc acgtcgccga
     5281 cgtggccgag cagcggatct acgaggtcgc tcggcacggc gagaaggagc aggtcgcctc
     5341 cctgcacgag ctcgtcgacg aagcgatgac ggatctcgaa cggatccaga accgcgagtc
     5401 cgcgttcgcg ggtgtgccga ccgggttccg cgacgtcgac accctcctgt cggggatgca
     5461 gtcgggaaac ctgatcgtga tcgcggcccg cccgggcgtg ggcaagtcct cgttcgtgac
     5521 caacgtcgcc cgcaacgtcg cggtcgacgc cggtctgccg gtcgcgatgt tctccctcga
     5581 gatgtcgcgg tgggagatcg gcatgcggtt gctctgcggt gaggccaggg tgccgtggga
     5641 cagcatccgc aacaagcgcg tgggcgcgga ggactggtcg cggatcgccc aggccgctga
     5701 cagcctgcac gaggcaccgc tgttcatcgt cgactcgggc aacgtcacgc tcgtcgacat
     5761 ccgcgccaag gcccgcaggt tgtccgcccg gagggcgggc ctcggcatga tcatcgtcga
     5821 ctacctccag ctgatgtccc acactcgccg cgtcgacaac cgccagcagg agatcgccga
     5881 gatcagccgt ggtctgaaga tgctcgccaa ggagctcgag atcccggtga tcgccgtctc
     5941 gcagctgaac cgcgaccccg aacgccgtca ggacaagcgc ccgcagcttt cggacctgcg
     6001 ggagtccggc gcgatcgagc aggacgccga cgtggtgatg ttcatccacc gcgaggacgc
     6061 ggaccctgcc aagaagggcc tggccgacct gatcgttgcc aagcatcgca acggtccgac
     6121 cgacacggta ccgctcacct tcctgccgca cctgacgcag ttccgcaacc acgccaccgc
     6181 ggtgacgtga ccgtcgaccg ccgccgcgtc
//
LOCUS       DATBRK010000393         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1238058, whole genome shotgun sequence.
ACCESSION   DATBRK010000393 DATBRK010000000
VERSION     DATBRK010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238058"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VIH70_11635"
     CDS             complement(<1..425)
                     /locus_tag="VIH70_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8117057.1"
                     /translation="MSSRRPLIETAHGTRREAFGPQEWGLLAAIAAMWGSSFLFIEIG
                     LEHFGPGVVAFGRVAVGALTLSLLRGSHRPVERSDLPRVALLGAVWMAAPLLLFPIGQ
                     QWIDSSLAGMLNGAVPIFAAVTAAILLRRLPASRQLIGIA"
     gene            complement(523..1599)
                     /locus_tag="VIH70_11640"
     CDS             complement(523..1599)
                     /locus_tag="VIH70_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY8117058.1"
                     /translation="MHRITLIPGDGAGPELVDAARVAVEATGVEIEWDVQPAGVSVIE
                     AEGTPLPPRVIESIRSNRVALKGPLATPAGFRSVNVGLRVTLGLYAAVRPCTLYPGVR
                     SRYERADVVVVRELTEGSYTGIELEQGAPQTAELIAFIERRTGKRVREDSGLSIKAIS
                     DTASRRIARFAFEYARANGRRKVTAGHKANIMKFSDGLFLEAAREVAREYPDVAFEDR
                     IIDNLAMQLVQRPEDHDVLLLPNLYGDILSDLCAGIVGGLGVAPGVNLGDAVAVFEAT
                     HGTASRYRGLHRANPMALMLCGSMLLRHIGETGAGDRLEAAVAAVIAEGRTVTYDLRP
                     TRDDPDAASTTDVRDAVVENLLSR"
     gene            1676..>2270
                     /locus_tag="VIH70_11645"
     CDS             1676..>2270
                     /locus_tag="VIH70_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY8117059.1"
                     /translation="MTDRGLREIVAAQTRLSDIDGRAGRLWYVGYDINDLAGRTSYEE
                     VVHLLHELRLPNQMELDALRDFMVEEREVSKFLRDDLMPTLAQQTSPMSMLRTSVSAA
                     SAYDPDGWDDSVDADRRKALRLIAKTPTLLAMYHRMRTGQEIVPPNTKLSHAANFLHM
                     LLGLEPSQEDAEVIDTTFVLYADHTMNASTFTARIVAS"
BASE COUNT          344 a          840 c          756 g          330 t
ORIGIN      
        1 gcgatcccga tcagttgccg gctcgcaggc agccggcgca gcaggatcgc ggccgtgacc
       61 gccgcgaaga tcggaaccgc tccgttgagc atgcccgcga gcgacgagtc gatccactgc
      121 tgcccgatcg ggaacagcag cagcggtgcg gccatccaga ccgcgccgag cagcgccacc
      181 cgtggcaggt cgctgcgttc gacgggccga tgggacccgc gcagcagcga gagcgtgagc
      241 gcgccgacgg ccacccgccc gaacgccacc acccccggcc cgaagtgctc gaggccgatc
      301 tcgatgaaca ggaacgacga gccccacatc gccgcgatcg cggcaaggag cccccattcc
      361 tgaggcccga acgcctcccg cctggtcccg tgggcggtct cgatcagcgg gcgtctgctg
      421 ctcatgtcgg ccctccgccc tccgctctgg ccgcccccgg gcagcctcct gctcgtgcga
      481 accctccggg gcccgtcagc cttcccaacg cgccacgagt gctcatcgtg agagcaggtt
      541 ctccacgacc gcgtcccgga catcggtggt gctcgcggcg tccgggtcgt cgcgcgtcgg
      601 ccgcaggtcg taggtgaccg tgcgaccctc ggcgatgacc gccgcgaccg cagcctcgag
      661 ccggtccccc gcccccgtct cgccgatgtg ccgcagcagc atcgatccgc acagcatcag
      721 cgccatcggg ttcgcgcggt gcaacccgcg gtagcgcgag gcggtcccgt gggtcgcctc
      781 gaacaccgcc acggcgtcgc cgaggttgac gcccggggcc acgccgagcc cgccgacgat
      841 ccccgcgcac aggtccgaga ggatgtcgcc gtagaggttc ggcaggagca ggacgtcgtg
      901 gtcctcggga cgctgcacga gctgcatcgc gaggttgtcg atgatccggt cctcgaacgc
      961 cacgtccgga tactcgcgtg ccacctcgcg ggcggcctcc aggaacaggc cgtcggagaa
     1021 cttcatgatg ttggctttgt gccccgccgt gaccttccgg cggccgttcg ctctcgcgta
     1081 ctcgaaggcg aaccgcgcga tcctgcggct cgcggtgtcg ctgatcgcct tgatcgacag
     1141 gcccgagtcc tcccgaacgc gcttgccggt cctccgctcg atgaaggcga tcagctccgc
     1201 cgtctgcggt gccccctgct cgagctcgat cccggtgtac gagccctccg tgagctcccg
     1261 aacgaccacg acgtccgcac gttcgtaccg ggaccgcacg ccggggtaca gcgtgcatgg
     1321 gcggaccgcc gcgtacaggc ccagggtgac ccggagcccg acgttcacgc tccggaagcc
     1381 ggccggcgtg gcgagcggac ccttcagcgc gacgcggttg ctccggatcg actcgatcac
     1441 ccgaggggga agcggcgtcc cctcggcctc gatgaccgag accccggcgg gctggacgtc
     1501 ccactcgatc tccacgccgg tggcctcgac cgcgacgcgg gcggcgtcca cgagctcggg
     1561 tccggccccg tcgccgggga tcagcgttat ccggtgcacc gtcacgcggg gattgtgatg
     1621 ggatgatggg cgaagcgacc atcatgaccc ttggcccgaa ggcggggagc gtccggtgac
     1681 cgacagaggc cttcgcgaga tcgtggcagc ccagacccgg ctgtccgata tcgacgggcg
     1741 ggcgggccgc ctgtggtacg tgggctacga catcaacgac ctcgccggtc ggacctccta
     1801 cgaggaggtc gtgcacctgc tccacgagct ccgcctgccg aaccagatgg agctggacgc
     1861 gcttcgcgac ttcatggtcg aggagcgcga ggtctcgaag ttcctgcggg acgacctcat
     1921 gcccacgctg gcccagcaga cctcgccgat gtcgatgctt cgtaccagcg tgtcggccgc
     1981 ctcggcctac gaccccgacg ggtgggatga ctcggtggac gcggatcggc gcaaggcgct
     2041 ccgcctgatc gcgaagaccc cgaccctgct cgcgatgtac caccggatgc ggacgggtca
     2101 ggagatcgtc ccgcccaaca ccaagctctc gcacgcggcg aacttcctcc acatgctgct
     2161 gggcctcgag ccgtcccagg aggacgccga ggtgatcgac acgacgttcg tgctgtacgc
     2221 cgatcacacg atgaacgcct ccaccttcac ggctcggatc gtcgcctcga
//
LOCUS       DATBRK010000394         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_800314, whole genome shotgun sequence.
ACCESSION   DATBRK010000394 DATBRK010000000
VERSION     DATBRK010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800314"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(390..1697)
                     /gene="mshA"
                     /locus_tag="VIH70_11650"
     CDS             complement(390..1697)
                     /gene="mshA"
                     /locus_tag="VIH70_11650"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HEY8117060.1"
                     /translation="MGLGNGRVTIERATDGPPVAIDAPRIAVVSLHTSPLDQPGSGDS
                     GGMNVFIRAAAERLSAGGLQVDVFTRTRDERRPEVEELGNGSRLINVQAGPSAPVPKD
                     ELPRFLPEFLGGVLRRARAEGSDYDLVHTHYWLSGWVGRSAREIWGVPLVASFHTLGK
                     VKNYSLSRGERPEPADRLSGEELVIADADRLIAATPVEAAQLVGLYRAEPDRIRIVPP
                     GVDHGLFFPRDRSRAKARLHLADLRLLLYVGRLQAHKGPDVAVRAVAEAIARDPAGTR
                     DVVLAIVGGPSGTDHGAEVARLMDLASVLGVGERVILFPPQPQSRLADFYAAAEAVLV
                     PSRSESFGLVALEAQACSTPVVASAVGGLRYVVEDGVTGHLVEGHDPGDHAERILAVL
                     GDPGERRRMGEAGVVRSLRFSWDATAAGMRSVYRELLDARRPA"
     gene            complement(1741..2724)
                     /locus_tag="VIH70_11655"
     CDS             complement(1741..2724)
                     /locus_tag="VIH70_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY8117061.1"
                     /translation="MPTAAPLVRVVRSGLVESVHLGHAVVCDPRGRLLAALGDPDRLV
                     FSRSSMKPLQAAVSLRRIAEPLPDDLVAVMCASHNGEPVHVRGVRRLLRTGGLSERDL
                     GCPPDLPMEPEARRAAAGPRRITHNCSGKHAGMLLACERSGAELASYLEPSHPLQREI
                     LRAVRSATGVQRPLVGVDGCGAPVHGLPLRGMATLFARLARPERLGRLGERAARAVAA
                     MRSAPYLVAGRGRSDTRLMDAVPGLVCKVGAEGLHCAAVLDAGIGVAVRIDDGSDRAA
                     APALVRTLDLLGVLDDGQRARLASVAAPPVLGGGRPVGRFEVGFRLPRRSG"
     gene            complement(2771..>3528)
                     /locus_tag="VIH70_11660"
     CDS             complement(2771..>3528)
                     /locus_tag="VIH70_11660"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEY8117062.1"
                     /translation="LPGHGPGVQTDPELPSVQEQLIVFARELGELYRLERSRNAELET
                     VLESLQDTYLATMKSLAIVIEAKDQTTRGHLDRTQVFGLALARRVDPELARSPTLGYG
                     FFLHDIGKVGIPEHILCKAGPLSIDEWTVMRNHPIIGAQIVAPIAFLSDTVELIRHHH
                     EWFDGTGYPDGLASEAIPLGARVFAVADSFDAMTSDRPYRGSIGPERALAELQGGAGT
                     QFDPEIVRVFVEMVERDPPEGDEDFAHALRHAH"
BASE COUNT          543 a         1247 c         1283 g          455 t
ORIGIN      
        1 tccccacccg cacagctagt tagctgtccc aaccgtacga gcaggtgcgc ccagtcggcc
       61 ttacggcttt cccaggggtc ccggcctcgg ttccctgatt cggccgctcc ggctgctcgg
      121 agcgagcgaa atgtaccggc ggcgctgagg ccggtcaacc cgacgaacgc gcaggtcacg
      181 gtacatatgc gcaggtagat ggcgtgatgg aaagagtgac aagaacatgt cccgaacgcg
      241 aacgtccctc gacgcatcgt tcggaaccct tgcggtgcag agcgttccac ggtgcccctt
      301 gaggcggatg ccggtcggag ccgtggcgcg cgcgcgcgaa tctccacgct gcacccggtt
      361 cgagcgcgcg acggcgtcgc cgctctcgtt caagcggggc ggcgggcgtc gaggagctcg
      421 cgatacaccg agcgcatgcc ggccgcggtg gcgtcccacg aaaagcggag cgagcgaacg
      481 acgccggcct cgcccatccg acggcgctcg cccggatcac cgaggaccgc gaggatccgc
      541 tccgcatggt cgccggggtc gtggccctcg acgaggtgcc cggtgacgcc gtcctcgacg
      601 acgtaccgga gaccgccgac cgccgacgcc actacggggg tcgagcaggc ctgcgcctcg
      661 agggccacga gcccgaacga ttccgacctc gaggggacca ggaccgcctc ggcggccgcg
      721 tagaagtccg ccaggcgcga ttgcggttgg ggcgggaaca ggatcacgcg ctcccccacg
      781 cccagcaccg acgcgaggtc catcagccgg gccacctcgg caccgtgatc ggtgccgctc
      841 ggaccgccga cgatggcaag cacgacgtcg cgcgtcccgg ccgggtcacg ggcgatcgcc
      901 tcggcgaccg ccctgacggc gacatcgggg cccttgtggg cctgcaggcg tcccacgtac
      961 agcagcagcc gcagatccgc gaggtggagc cgcgccttgg cccggctccg atctcgaggg
     1021 aagaacaggc cgtgatcgac gcccgggggc acgatccgga tgcggtcggg ctcggcccgg
     1081 tacaggccga cgagctgcgc cgcctcgacc ggcgtggccg cgatgagccg gtccgcgtcc
     1141 gcgatcacca gctcctcgcc cgacagacgg tccgcaggct cgggccgctc gccgcgcgac
     1201 agggagtagt tcttcacctt gccgagggtg tggaacgagg ccaccagcgg gacgccccag
     1261 atctcgcggg cgctgcgccc cacccagccc gagagccagt aatgcgtgtg gacgaggtcg
     1321 tagtccgagc cctcggcccg agcccgccgg agcacacccc cgaggaactc cgggaggaac
     1381 ctcgggagct cgtccttcgg caccggggca ctcggcccgg cctgcacgtt gatcagccgg
     1441 gagccgttcc cgagctcctc gacctcggga cgacgctcgt cgcgagtgcg cgtgaagacg
     1501 tccacctgca ggccgcccgc cgacaagcgt tcggccgccg cccggatgaa gacgttcatc
     1561 ccgcctgaat cacccgaacc cggctggtcg agcggggatg tgtgcaggct gaccacggcg
     1621 atcctcggcg cgtcgatggc caccgggggc ccgtccgtcg cgcgctcgat ggtgactcga
     1681 ccgttgccca agcccatgca ggaatcgtac gggagggcgc tccgaatgca gggaaggggc
     1741 tcagccggac cggcggggca gccggaaccc gacctcgaac cggcccacgg gccggccgcc
     1801 accgagcacg ggaggtgccg ctacggacgc cagccgggcg cgctgtccat cgtcgagaac
     1861 tccgagcagg tcgagcgtcc ggaccaacgc cggggcggcg gcgcgatcgc ttccgtcgtc
     1921 gatccgcacg gcgaccccga tcccggcgtc gaggacggcc gcgcagtgca gaccctcggc
     1981 ccctaccttg cagacgaggc cggggaccgc atccatcagc cgtgtgtccg aacggccgcg
     2041 ccccgcgaca aggtacgggg ccgaccgcat cgccgcgacg gcgcgggcgg cgcgctcgcc
     2101 cagtcggccg agccgctcgg gcctcgcgag ccgtgcgaac agcgtcgcca tacctcgcag
     2161 cggcagcccg tggaccgggg cgccgcaccc gtcgaccccg accaacggtc gttggacgcc
     2221 ggtggccgaa cgcaccgcgc gcaggatctc gcgctggagc ggatgggacg gttcgagata
     2281 gctcgcgagc tcggcgcccg agcgttcgca cgcgaggagc atgccggcgt gcttgcccga
     2341 gcagttgtgg gtgatccgtc ggggaccggc agccgcccga cgcgcctcgg gttccatcgg
     2401 caggtccggg gggcacccca ggtcgcgttc ggacaacccg ccggtccgaa ggagtcgccg
     2461 gacgccccga acgtggactg gctcgccgtt gtgcgacgcg cacatgacgg cgaccaggtc
     2521 gtcggggagc ggctcggcga tccggcgcag ggacacggcc gcctgcaggg gtttcatcga
     2581 cgagcgtgag aacacgagac ggtccgggtc gccgagggcg gccagcaggc ggcctcgcgg
     2641 atcgcacacg accgcgtggc cgaggtggac gctctcgacg agacccgaac gcacgacccg
     2701 gacgagcggt gcggcggtcg gcacacgcgc agcctagcga gccgagcgcg gttcggcggg
     2761 atccgatggg tcagtgggcg tgcctgaggg cgtgcgcgaa gtcctcgtcg ccctcgggcg
     2821 gatcgcgctc caccatctcg acgaagacgc gaacgatctc ggggtcgaac tgggtcccgg
     2881 cgccgccctg gagctcggcg agcgcgcgtt cggggccgat cgagccgcgg tacggacggt
     2941 cgctggtcat cgcgtcgaac gagtcggcca cggcgaacac gcgcgcgccg agcgggatcg
     3001 cttcggacgc cagcccgtcg gggtagccgg ttccgtcgaa ccactcgtgg tggtggcgga
     3061 tcagctcgac ggtgtcggaa aggaacgcga tcggcgcgac gatctgggca ccgatgatcg
     3121 ggtggttgcg catcaccgtc cactcgtcga tcgagagcgg acccgccttg cacaagatgt
     3181 gttcggggat cccgaccttg ccgatgtcgt ggaggaagaa cccgtacccc agggtcggcg
     3241 agcgcgccag ctcggggtcg acccgccggg cgagcgcaag cccgaacacc tgggttcgat
     3301 caagatggcc gcgggtggtc tggtccttgg cttcgatcac gatcgcgagg ctcttcatcg
     3361 tggcgaggta ggtgtcctgc aggctctcca ggaccgtctc gagctcggcg ttgcgggatc
     3421 gttcgaggcg atagagctcc cccagctccc gggcgaagac gatgagctgc tcctggacgc
     3481 tcggcagttc ggggtccgtc tggaccccgg ggccgtgccc gggaaggt
//
LOCUS       DATBRK010000395         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1381169, whole genome shotgun sequence.
ACCESSION   DATBRK010000395 DATBRK010000000
VERSION     DATBRK010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381169"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /gene="pilM"
                     /locus_tag="VIH70_11665"
     CDS             complement(<1..547)
                     /gene="pilM"
                     /locus_tag="VIH70_11665"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY8117063.1"
                     /translation="MARTRIGVDVGSTAVRVAEVAAGDIPVIVRAAQVPLPPGVVEAG
                     EVRQPEAVAEALRELWSKSGVKSKQVRLGVGNQRVVVREVVLPWLPEKELRDTLGFQV
                     QEFIPMASDEAVLDFDFMGEMDQGGRRMMRILLVAAHKPMVNALVEAALAARLDPQGI
                     DLSPFAVIRAVGTGDLGLDLNS"
     gene            complement(547..771)
                     /locus_tag="VIH70_11670"
     CDS             complement(547..771)
                     /locus_tag="VIH70_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8117064.1"
                     /translation="MAKRSQSGWVGRQAGDRLLTVAEVAATMRVSNMTVYRLIKNGDL
                     PALRVGKNYRIRESDVDVYLSDRSVEVEGA"
     gene            complement(781..972)
                     /locus_tag="VIH70_11675"
     CDS             complement(781..972)
                     /locus_tag="VIH70_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016189267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8117065.1"
                     /translation="MSKRRPFVGDRLLTVGEVAATMRVSNMTVYRLIKSGQLAAIRVG
                     KNYRIRESDVDRYLSERSV"
     gene            complement(1209..>1663)
                     /locus_tag="VIH70_11680"
     CDS             complement(1209..>1663)
                     /locus_tag="VIH70_11680"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY8117066.1"
                     /translation="GLMVAISVIDIRHRRIPNRLVYPAFVIAAAVIIVGGLAGGGLDA
                     LDGGIGLFAYGLGLMIIALISPKGMGMGDVKLAGLIGLVLGSIGLDLVAVAAGMGILL
                     GGAGAIVALLTGAGRKSAIPFGPFLAAGAVIAIFVGRPIADAYLNLLT"
BASE COUNT          297 a          614 c          498 g          254 t
ORIGIN      
        1 tcgagttcag atccaggccc aggtcccccg tccccacggc cctgatcacc gcgaacgggg
       61 acaggtcgat gccctgggga tcgagcctgg ccgccagggc ggcctcgacc agggcattga
      121 ccatcggctt gtgcgccgcc accaggagga tccgcatcat ccgccgtccc ccttgatcca
      181 tctcgcccat gaagtcgaag tccaggacgg cctcgtccga ggccatcggg atgaactcct
      241 ggacctggaa ccccagcgtg tcccgcagct ccttctcggg cagccagggg agaacgacct
      301 cacggaccac cacgcgctgg ttcccgacgc cgaggcggac ctgcttgctc ttcaccccgg
      361 acttggacca gagctcgcgg agcgcctcgg ccacggcctc gggctggcgg acctcgccgg
      421 cctccacgac accgggcggg agcggcacct gggccgcccg aacgatcacc gggatgtccc
      481 cggcggcgac ctcggcgacg cgcaccgcgg tcgacccgac gtccacgccg atcctggtcc
      541 tggccactac gcgccctcca cctcgacgga ccgatcggag aggtagacgt ccacgtccga
      601 ctcgcggatg cggtagttct tccccactcg caaggccggc aggtcgccgt tcttgatgag
      661 ccggtagacg gtcatgttcg agacgcgcat ggtggccgcg acctcggcga cggtcagcaa
      721 gcggtctccc gcctgtcgac ctacccatcc gctctgagac cgtttggcca tcgttcccac
      781 ctacaccgat cgctccgaca gataccgatc gacgtccgac tcacggatcc ggtagttctt
      841 tcccacgcgg atcgccgcca gctgaccgct cttgatcagc cggtacacgg tcatgttcga
      901 cacccgcatc gtcgccgcga cctcgcccac cgtgaggagc cggtcaccca cgaagggcct
      961 ccgtttgctc atctagcgca tcccccacat ctgcctcatg ttcaacagtt tccacatccg
     1021 cctcatccat cccatcccgc ccgcaggggt gcgcaccgta acaagtgcaa catctatcgt
     1081 caatcacccc atgtgcctac tttcggcacg gtaggctaaa tcacatagat gtaattagct
     1141 cttcttccca agagtcacag gagtcacagg accggggtgc cgcgcgacga ggccggcggg
     1201 cgtgtggctc aggtgagcag gttcagatag gcgtcggcga tcgggcgacc tacgaagatg
     1261 gcgatcacgg ccccggcggc gaggaacggt ccgaacggga tcgccgactt cctgcccgcg
     1321 ccggtcagca gggcgacgat ggcccccgct cccccgagca ggatccccat gccggccgcg
     1381 accgcgacga ggtcgaggcc gatcgagccg aggaccaagc cgatcaggcc ggcgagcttc
     1441 acgtcaccca tccccatgcc cttcggcgag atcagggcga tgatcatcaa gccgagccca
     1501 taggcgaaca gcccgatccc cccgtccagg gcgtccagtc caccaccggc cagaccgccg
     1561 acgatgatga ccgcggccgc gatcacgaac gccgggtaga ccagacggtt cgggatgcgg
     1621 cggtgacgga tgtcgatcac ggagatggca accatgagcc cgg
//
LOCUS       DATBRK010000396         7221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_944551, whole genome shotgun sequence.
ACCESSION   DATBRK010000396 DATBRK010000000
VERSION     DATBRK010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944551"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VIH70_11685"
     CDS             complement(<1..440)
                     /locus_tag="VIH70_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8117067.1"
                     /translation="MAFEAHPVTKERWPDLVELFDRPIVRTCFCMFYRKVGAGTGPGQ
                     QNRRAMKALVDRGSVPGLIGYEDGVPVAWVSLGPREDYAKLRRSPVMKPVDDRPVWSI
                     VCFFVDSKARGRGLSQRMLEAAVDYARSRGARLVEAYPVDKDQR"
     gene            572..2380
                     /locus_tag="VIH70_11690"
     CDS             572..2380
                     /locus_tag="VIH70_11690"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY8117068.1"
                     /translation="MRLPRGSFRFAVGFALATTGLIVTALPVAAATDAEVTVGSSNPF
                     SGNKQNEPTVAVDPNHHNLLVAGANDNIDLEDCNAGADSSCPFTAGVGTTGIQYSIDG
                     GLTWQQPTFTGLSARNCTGVNDPPGAVPTDAGVPQEGGPIGTLPWYFENGLVSDGDPA
                     VAWGPSPGPNGFDWSNGSRLYFANLTSNVASKHSEEGFKGVEALGVSRIDVTSAVAAT
                     QAARLADEARWMPPSLIPSGTSGFSDKEQIWSDTAASSEFFGNVYVCYGDFSGGPSAG
                     SNSARLKVARSTDGGTAWSQVVVEKNTDAASGNWGLASGATGCTMRTDSKGTVYLFWH
                     GFNMKTKEEGIFLSRSFDGGATFEGRRRLFLVHPTGVFDPVLGRNTMDGIAGARADLS
                     TAPSVDIANGSPTGSGATDRIVLTWIDGQTLNDEHVRFSTSVDGGDTWTAPVNVETSA
                     SDRGLYAAAAVTPDGTDVWLVYNAFTTPYRTTTSAARRLVGVVAHANVTGGLVGPFGE
                     MHRSTPGDARGSTQNDLTGEFLGDYVYATATNDFGAFVWNDVRTATDCPPVDAWRIAL
                     RTKDKKDDPPRPEPNNDCNASFGNSSIFGAAIADPT"
     gene            complement(2397..3593)
                     /locus_tag="VIH70_11695"
     CDS             complement(2397..3593)
                     /locus_tag="VIH70_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8117069.1"
                     /translation="MTGYSREDAAERVGVEPSYLVRLVDLGIIVPEESDRFSPGDVRR
                     VLLARSLEDAGISLEEVAAAIRRGALSLGFLDAASYERFAALAAETFRQVSDRTGIPL
                     DLLMVIREAIGMAQPSPDDRLREDELAIVPFIELQLAEGFRPVAIERLLRVQGDGTRR
                     ITEQEAAWWTSEVIEPAIAAGKGPEEIGNAELADRAAVGSEQALLAMYHAQQAHAWTA
                     NIIEGFEVLMANAGIRSRLERPPAICFLDITGYTRLTQERGDDAAADLSATLARLVQR
                     SSVQRGGRPIKWLGDGVMLYFGDPGAGVNAALEMLDGLAAAGLPPAHVGLHAGPVLFQ
                     EGDYFGQTVNLSARIADYARQGEVLVSQAVAEASPDRGITFADIGPVELKGVAGTVHL
                     LRAQLA"
     gene            3796..4974
                     /locus_tag="VIH70_11700"
     CDS             3796..4974
                     /locus_tag="VIH70_11700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY8117070.1"
                     /translation="MSEPSEHRERAFAWKPVLVTAAAVVLLLLAVAGRYGYHRDELYF
                     LACGRRLAWGFVDQPPLTPALARLSEILFPDSLVGIRLWPALMVGGLVAVTSLTAQEL
                     GAGRTGQIAVAVCAACTPGFLLAGHLLSTETFDLVAWAVTTLLVIMIVRRGEPRLWLG
                     VGVVVGVGLENKWSIMFLVVGLLIGLLATPERRVLWTPWFAAGVAVALVMWIPNLAWQ
                     ADHGWPQIGMIRVIQSDSMDLGSTIAWIPLQVVITGFVPAIVWIAGLLRVLRDPETRP
                     YRFVGIAYVALAVVFAVSAGDKSYYVAGLYASLYGAGAVPLERWLSRHPRGIARPTAL
                     AALALTTFLLLPVAVPVLPVTALADSSINDLDPEMGEQVGWPELVHQVARVWDRIPVA
                     "
     gene            5050..5304
                     /locus_tag="VIH70_11705"
     CDS             5050..5304
                     /locus_tag="VIH70_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117071.1"
                     /translation="MPQPYSAHNNYWWWGPPPWDTRVAIIVGWWSDAYAGRFFGSVER
                     AGTIRNAFGVDNDEEGAPIWLASDARRSLPAMWPEMRSYG"
     gene            5440..5775
                     /locus_tag="VIH70_11710"
     CDS             5440..5775
                     /locus_tag="VIH70_11710"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8117072.1"
                     /translation="MEPHPGVFVSEASTDDWEPDPEVPGSEMHELVHADGVWAGLTRL
                     TSVDGPVPWTPERREVALILEGSVRIEIADGDTLDLGVGDLFSLPPGVATTWHVTTPF
                     QEMWVLASD"
     gene            5852..6382
                     /locus_tag="VIH70_11715"
     CDS             5852..6382
                     /locus_tag="VIH70_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HEY8117073.1"
                     /translation="MDWAVLTYRVPQDPSRHRVAIWRELRRAGAVSLQQATWALPLHP
                     ATEATIDRVLALVHRAEGEAFVFTASPRDQAMAGSIEESFLEGREAEWHEFVRECDKF
                     DAEIAHEIASEKFTPAELDEEEQNLERLQRWYRALRSRDVLGAPSAPDAELRLKSSSE
                     RLEEFAQQVFERGGGA"
     gene            6382..6618
                     /locus_tag="VIH70_11720"
     CDS             6382..6618
                     /locus_tag="VIH70_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117074.1"
                     /translation="MRQTTSRIRDIVKRTPEPEVITRGTWESLRTHAARRAHEALDEV
                     PGVLRRLKTFLLLGSITMIAFAAGMLVVLWHAVR"
     gene            6748..7041
                     /locus_tag="VIH70_11725"
     CDS             6748..7041
                     /locus_tag="VIH70_11725"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEY8117075.1"
                     /translation="MLLLFKDLVRDERVPRGAKLWLGFAAIWLASPIDLIPEFIPVLG
                     PLDDAVVAAAVLRHLVRKAGSEIVYEHWRGEPSTISRVLRLFGADSPSREGEG"
     gene            complement(7038..>7221)
                     /locus_tag="VIH70_11730"
     CDS             complement(7038..>7221)
                     /locus_tag="VIH70_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117076.1"
                     /translation="AASHIVSLRHAEHDPGVTAERLVSEARIVCGVRAGGLRISLHAY
                     NDGDDVDRLVEALRRL"
BASE COUNT         1131 a         2449 c         2456 g         1185 t
ORIGIN      
        1 ctgcgttggt ccttgtccac cggatacgcc tccacgaggc gggcgccgcg agaccgcgca
       61 tagtccacgg cggcctcgag catccgttgt gataggcctc gacctcgagc cttgctgtcg
      121 acgaagaagc agacgatgga ccacaccggc cggtcgtcca ccggcttcat caccggggac
      181 cggcggagct tggcgtagtc ctccctgggc cccagcgaca cccatgcgac cggaacaccg
      241 tcctcgtatc cgatcaaccc gggcacggag ccccgatcca cgagtgcctt catcgcgcgc
      301 cggttctgct ggccggggcc ggtgccggcc ccgaccttcc tatagaacat gcagaagcag
      361 gtgcgaacga tgggccgatc gaacagctcg accaggtcgg gccaccgttc cttcgtgacc
      421 gggtgtgctt cgaacgccat cggggatcaa tctaccgggc gtcaggaccc accgacgatc
      481 gcctgaacgc cgcccgtccg ttgccccttt cctgggacgt gtgcaagggt gatgacgaac
      541 tcgctaccgc acccggaagg agatggcgcg ggtgagactt cctcgcggat cgttccgctt
      601 cgcagtcgga ttcgcgctcg cgacgacggg gctgatcgtc accgcgctgc cggtcgcggc
      661 ggcgaccgac gcggaggtca ccgtcgggag ctcgaacccc ttcagcggga acaagcagaa
      721 cgagcccacg gtcgccgtcg atcccaacca tcacaacctg ctggttgccg gcgcgaacga
      781 caacatcgac ctcgaggact gcaacgccgg cgcggacagc agctgcccgt tcacggcggg
      841 tgtcggaacc acggggatcc agtactccat cgacggcggg ctcacctggc agcagccgac
      901 cttcaccggc ctcagtgccc ggaactgcac cggcgtgaac gacccacccg gcgccgttcc
      961 gacggacgcc ggcgtgcccc aggagggagg gccgatcggg accctgccct ggtacttcga
     1021 gaacggtttg gtctccgacg gcgacccggc cgtggcctgg ggaccgtcac ccggtccgaa
     1081 cggcttcgac tggtcgaacg gctcccgtct gtacttcgcg aacctgacct cgaacgtcgc
     1141 ctccaagcat tcggaggaag ggttcaaggg cgtcgaggcc ctcggcgtct cgaggatcga
     1201 cgtgacctcg gcggtcgccg cgacgcaggc ggcacggctc gccgacgagg cacggtggat
     1261 gccgccgtcg ctgatcccct ccgggacctc cggcttctcc gacaaggagc agatctggtc
     1321 cgacaccgct gcgagcagcg agttcttcgg caacgtgtac gtgtgctacg gcgacttcag
     1381 cggcgggccg agcgccggct cgaactccgc tcgtctgaag gttgctcggt ccacggacgg
     1441 cggtaccgcc tggtcgcagg tagtcgtcga gaagaacacc gacgcggcgt cgggcaattg
     1501 ggggcttgcg tcgggcgcca ccggttgcac gatgcggacc gacagcaagg gcacggtcta
     1561 cctcttctgg cacgggttca acatgaagac caaggaagaa gggatcttcc tctcccgctc
     1621 gttcgacgga ggcgccacct tcgagggccg ccggcgcctg ttcctcgttc acccgaccgg
     1681 cgtcttcgac cccgtgctcg gccgcaacac gatggacggg atcgcggggg cacgcgccga
     1741 cctctcgacc gcgccgagcg tcgacatcgc caacggctca cccaccggat cgggtgctac
     1801 cgaccggatc gtgctgactt ggatcgacgg ccagaccctc aacgacgaac acgtgaggtt
     1861 ctccacctca gtcgacgggg gggacacctg gacggcaccg gtcaacgtcg agactagcgc
     1921 ctccgaccgt gggctctacg ccgccgccgc ggtcacgccg gacgggaccg acgtgtggct
     1981 cgtgtacaac gccttcacca ccccgtaccg gacgacgacg tcggcagccc ggcggctcgt
     2041 cggtgtggtc gcgcacgcca acgtgaccgg cggcctcgtc ggaccgttcg gcgagatgca
     2101 ccgaagcaca cctggtgacg ctcgtggatc cactcagaac gatctgacgg gggagttcct
     2161 cggtgactac gtctacgcga cggcgacgaa cgacttcggc gcgttcgtgt ggaacgacgt
     2221 gcggacggcc acggactgcc cgcccgtgga tgcctggcga atcgcgctca gaaccaagga
     2281 caagaaggac gacccgccca ggcccgaacc gaacaacgac tgcaacgcga gcttcggtaa
     2341 ctcgagcatc ttcggagcgg cgatcgccga cccgacatag ggcggggggt cgatgattaa
     2401 gcgagctggg cccgcagcag atgcaccgtt ccggctacgc ccttgagctc caccggaccg
     2461 atgtcggcga acgtgatccc cctatccggc gaggcctcgg cgactgcctg gctgaccagt
     2521 acctcgccct ggcgcgcgta gtcggcgatg cgcgccgaca ggttcacggt ctggccgaag
     2581 taatcgccct cctggaacag gaccggcccc gcgtgcaggc cgacgtgggc cggcggcaac
     2641 ccggcggcgg cgagaccgtc cagcatctcg agggcggcat tcactcccgc gccggggtct
     2701 ccgaagtaga gcatcacgcc gtcacccagc catttgatcg gcctgccacc gcgctgcacg
     2761 gaactgcgct gcaccagccg tgcgagcgtc gccgacaggt cggcggccgc atcgtcgccg
     2821 cgttcctggg tcagccgcgt gtagccggtg atatccagga agcagatcgc cggtggtcgc
     2881 tcgagacggc tgcggatccc tgcgttcgcc atcagcacct cgaagccctc gatgatgttg
     2941 gcggtccagg cgtgcgcctg ctgcgcgtga tacatggcca ggagggcttg ctccgaaccc
     3001 acggcagccc ggtcggcgag ctcggcgttc ccgatctcct ccgggccctt gcccgccgcg
     3061 atggccggct cgatcacctc ggaggtccac caggccgcct cctgttcggt gatccgccgg
     3121 gtgccgtccc cctggacacg gagcagacgc tcgatcgcga ccggccggaa tccctcggcc
     3181 agctggagct cgatgaacgg aacgatcgcc agctcgtcct cgcgaagccg gtcatcggga
     3241 gacggctggg ccatgccgat cgcttcacgg atgaccatca gcaggtcgag cggaatcccg
     3301 gtccgatcgc ttacctgccg gaacgtctcg gcggcaaggg cggcgaaccg ttcgtagctg
     3361 gccgcgtcga ggaagccgag cgacagggca ccacgccgga tggcggcggc cacctcttcg
     3421 agcgagatcc cggcatcctc gagcgatctg gcaagcagca cgcggcgcac gtcgcccggc
     3481 gagaaacgat ccgactcctc tgggacgatg atgccgagat cgaccagtcg aacgaggtag
     3541 gagggctcga cccccacccg ttcggcagcg tcttcgcgtg agtacccggt cacgttcggc
     3601 aagcctactg cggctgcgat ccttcacctg ccaccggcca ggctgactcg gaagcgatac
     3661 ccgatggcgg gcgagtcgaa tggttaccgt ggcgatcgcg atcgtccgcg agccgatacg
     3721 ggcagcgacc tctcgaaggc gtcctgggcc gtgactcgac agcgatcgcg tcgcggcata
     3781 tcaacagcac tcgtgatgag cgagccgtcg gagcatcggg agcgcgcatt cgcgtggaag
     3841 ccggtcctgg tgaccgcggc cgccgtcgtg ctgctgctgc tcgcggtcgc cgggcggtac
     3901 ggttatcacc gcgacgagct ctacttcctg gcgtgcggcc gccgtctggc gtgggggttc
     3961 gtcgaccagc cgccgctcac accggcgctc gcccgcctgt ccgagatcct gttcccggac
     4021 tccctcgtcg ggatccgcct gtggccggcg ctcatggtcg ggggactcgt cgcggtcacc
     4081 tcgctcaccg cgcaagagct cggggccggc cgaaccggtc aaatcgccgt cgcggtctgc
     4141 gctgcatgca ctcctgggtt cctcctcgcg gggcatctgc tgagcaccga gacgttcgat
     4201 ctggtcgcgt gggccgtcac gaccctgctc gtgatcatga tcgtgcgccg cggggaacca
     4261 cgcctctggt tgggcgtcgg tgtagtcgtc ggcgtcggcc tggagaacaa gtggtcgatc
     4321 atgttcctcg tggtcggcct gctgatcggt ctgctggcga cgcccgagcg ccgcgtgctg
     4381 tggacgccat ggttcgccgc cggcgtcgcg gtggccctgg tgatgtggat cccgaacctt
     4441 gcatggcagg cggaccacgg ttggccgcag atcggaatga tccgcgtgat ccagtccgac
     4501 agcatggacc tgggcagcac gatcgcgtgg atcccgctgc aggtggtgat cacgggtttc
     4561 gtcccggcga tcgtgtggat cgccggcctg ctccgcgtcc tccgcgaccc cgaaacgcgt
     4621 ccgtaccgat tcgtcggcat cgcctacgtg gcgctggccg tcgtgttcgc cgtctctgcc
     4681 ggtgacaagt cctactacgt ggccgggttg tacgcgtccc tctacggtgc gggggccgtt
     4741 cccctcgagc gatggctctc gcggcaccca cgcgggatcg cccgacccac ggcgttggcg
     4801 gcgctcgcgc tcacgacgtt cctgctgttg ccggtcgctg taccggtgct cccggtgacg
     4861 gccctggcgg actcctcgat caacgatctc gatcccgaga tgggggagca ggtcggctgg
     4921 cccgagctcg tccaccaggt cgctcgggtg tgggatcgga tccccgtagc ctagcggtcg
     4981 tcggcgatca tcctgacgac gaactacggc gaggcaggcg cgatcgagcg gttcggaccg
     5041 ggcctcgggt tgccccagcc gtacagcgcc cacaacaact actggtggtg gggcccgccg
     5101 ccctgggaca cgcgcgtcgc catcatcgtg ggctggtggt cggacgcgta tgccggccgg
     5161 ttcttcgggt cggtcgagcg tgccgggacg atccgtaacg cgttcggggt cgacaacgac
     5221 gaggaaggtg cgccgatatg gctcgcatcc gatgcacgac ggtcgctgcc cgcgatgtgg
     5281 ccggagatgc ggagttacgg ctgagcgcat cctccccgaa cacgggtgga gataacgacc
     5341 cttcggtgtt gttgcttcat ccctacgtgg gccgccggtc gctcatgacg ggttggcgtg
     5401 ctccctcagg tgacgtgatg attcgaagga gggcgaagga tggagccgca ccctggtgtg
     5461 ttcgtctcgg aggcctcgac cgacgactgg gagcccgacc ccgaggtgcc cggcagcgag
     5521 atgcacgagc tggtgcacgc cgacggcgta tgggccgggc tgacgcgttt gacgagcgtt
     5581 gatggcccgg tgccgtggac tcccgagcgt cgagaggtcg cgctgatcct ggaggggtcc
     5641 gtgcggatcg agatcgcgga tggtgacacg ctggatctcg gggtggggga tctgttctcg
     5701 cttccgcccg gcgtcgcgac cacgtggcac gtcacgacac cgttccagga gatgtgggtg
     5761 ctcgcttcgg actgactccc ccggcccgga cccgctcgac ggccgtgttg ggtaacactt
     5821 gttacgaata tgatgtaaca ttcgctacac catggactgg gcggtcttga cctaccgggt
     5881 gccgcaggat ccgtcacggc accgcgtcgc catctggcgc gagctccgtc gagccggcgc
     5941 cgtgtcgctg cagcaagcga cgtgggccct cccgctccat ccggccacgg aggcgacgat
     6001 cgatcgtgtg ctggccctcg tgcatcgtgc cgagggagag gcgttcgtct tcacggccag
     6061 cccgcgggac caggcgatgg cgggcagcat cgaggaatcg ttcctcgagg gccgagaggc
     6121 ggaatggcat gagttcgtca gggaatgcga caagttcgac gccgagatcg cccatgagat
     6181 cgcatcggag aagttcacgc cggccgagct cgatgaggag gaacagaacc tcgagcggct
     6241 gcaacgctgg taccgagcgt tgcgttcgag agatgttctc ggcgcgccct cggctcccga
     6301 tgcggagctc cggctcaagt ccagctccga gcggctcgag gagttcgcgc aacaggtgtt
     6361 cgagcgggga ggaggagcat gatgcggcag actacgagcc ggatccgaga catcgtgaag
     6421 cgcacgccgg agccggaggt gatcacccgt ggcacctggg agagcctgcg cacgcatgcc
     6481 gcacgccgcg cccacgaggc gctcgatgag gtgccgggag tgctccggcg cttgaagacc
     6541 ttcttgctgc tgggatcgat cacgatgatc gcgttcgcgg ccgggatgct cgtcgtcctt
     6601 tggcacgcgg ttcggtgagg acctgacctg cacccgctcc tggtcggcct cgcgatcgct
     6661 ctcggcctgt gggccgcggc gatcgtcacg ctcgtcgcga tcggtcgcat cgcggtcgcg
     6721 cgcgagctca tcggcctgat ccccaacatg ctgctcctgt tcaaggacct cgtccgcgat
     6781 gaacgggtcc ctcgaggcgc gaagctgtgg ctcgggttcg ccgccatctg gctcgcatca
     6841 ccgatcgacc tgatccccga gttcatcccc gtcttgggcc cgctggacga cgcggtggtc
     6901 gccgccgctg tgctccgcca cctcgtacgc aaggctggga gcgagatcgt gtacgagcat
     6961 tggcgcggag aaccatccac gatctcgcgg gtcctccggc tcttcggtgc tgactcgccg
     7021 agccgagaag gcgagggcta gagacgcctc agggcctcga cgagccgatc gacgtcgtcg
     7081 ccgtcgttgt aggcgtggag cgagatcctg agtccgcccg cgcgaacgcc gcagacgatc
     7141 cgggcctccg aaacaagacg ctccgcggtc actcccggat cgtgttcggc gtgccgaagg
     7201 gacacgatgt gggatgccgc c
//
LOCUS       DATBRK010000397         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_666159, whole genome shotgun sequence.
ACCESSION   DATBRK010000397 DATBRK010000000
VERSION     DATBRK010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666159"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VIH70_11735"
     CDS             <1..325
                     /locus_tag="VIH70_11735"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEY8117077.1"
                     /translation="RKTGRDWMTKALGQMNPGSTAKAKLSRYNFAGMGPWMLGKVAED
                     YKTPHPTELLELAREMGVRLIPCQMTMDLMGIGTEDLIDGLQEPIGAATALLEMKESA
                     IQLFI"
     gene            417..881
                     /locus_tag="VIH70_11740"
     CDS             417..881
                     /locus_tag="VIH70_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8117078.1"
                     /translation="MTKIAPIDAPALAARLRLGVTRLARRLRQEAEAGVTPSMLSALS
                     SADREGPLTMRELCRAEQVQPPTMTRIVAALVEAGLVVRDPDPADGRVAWVRVTPEGR
                     RLLERSRRRKEAYLAKALRSLDPVELRTLEAAAEILERLTDVPSATRSGART"
     gene            878..2140
                     /locus_tag="VIH70_11745"
     CDS             878..2140
                     /locus_tag="VIH70_11745"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8117079.1"
                     /translation="MTPVRVTFHRTFHSLGHSANFRRFFIGQAVSVTGTWMQMVAAAW
                     LVLQLTGSGVALGIDTALAFGPILLFGPLGGSLADRYDKRRILLATQVTFGALALMLW
                     GIVATGVVQLWMVYALSFLQGVTTSIDQPARQSFFAEMVRDRDLPNAISLNSAVMTGT
                     RIVGPALAGVLIAGVGLPWCFFINAWSYLAVVVALLSMHADQLRAQRAPREAGAIREG
                     LRYAWRTGELRRPLLLMSVLFLFSFNYSVLMPLFAKRTFGGNAGTLALLFSVAGVGSL
                     AGALVMAGRPRPGERRLALAAVGVGVVTILVALAPSLDVAVVSMLPLGVASIVFFVTA
                     NSTLQLTSRPDMRGRVMALYGIVFLGTTPFGAPMAGWVGEHLGPRIAFAGGGVIALAA
                     GLVGLYLLSRRAAADRFAVEPVAAAETA"
     gene            complement(2184..>2789)
                     /locus_tag="VIH70_11750"
     CDS             complement(2184..>2789)
                     /locus_tag="VIH70_11750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8117080.1"
                     /translation="VTELLARRLPRGRVVALDGSSAMLGTARRRLAPFGDRITFVHAE
                     LLDPLPLAEPVDAVLSTATFHWVPDHDALFANLAAVLRPGGRLVAQCGGDGNLAGVVR
                     VLDELGAAPFTGKVFATPERTEKRLRAAGFARAECWLHAEPTPFESLEALETFLHTVV
                     LRDHVRSMTDAEADAFAHAVAARLPRLELDYVRLNIDALRS"
BASE COUNT          360 a         1005 c          999 g          425 t
ORIGIN      
        1 ccgcaagacc gggagggact ggatgaccaa ggcgctcggc cagatgaacc ccggttcgac
       61 ggccaaggcc aagctctccc ggtacaactt cgccggcatg ggcccctgga tgctcggcaa
      121 ggtggccgag gactacaaga cgccgcaccc gaccgagctg ctcgagctcg cgcgcgagat
      181 gggcgtccgc ctgatcccct gccagatgac gatggacctg atggggatcg gcaccgagga
      241 cctgatcgac gggctgcagg agcccatcgg cgccgccacc gcgctgctcg agatgaagga
      301 gagcgcgatc cagctgttca tctagcgggc cgttcacgcc tcggccggtc tcgcaggact
      361 ggccacaccc gtgtcgtgcg cgacgctaat tagcgttgcg tagtatcatg atgggcgtga
      421 ccaagatcgc tcccatcgac gcacccgccc tcgccgcccg gcttcgcctc ggcgtcacgc
      481 ggctcgcgcg gcggctgcgg caggaggccg aggcgggagt caccccatcg atgctctcgg
      541 cactctcgtc cgccgaccgc gagggccccc tcacgatgcg cgagctctgc cgggccgagc
      601 aggtccagcc cccgacgatg acccggatcg tcgccgccct cgtcgaggcc gggctcgtcg
      661 tccgcgaccc cgacccggca gacgggcgcg tggcctgggt gagggtgacg ccggagggac
      721 gtcggctgct cgagcggagc cggcgccgca aggaggccta cctggccaag gcgctccgct
      781 cgctcgatcc tgtggagctc cggaccctcg aggcggccgc cgagatcctc gagcgactga
      841 ccgacgtgcc gtcagccacg cggtccgggg cacgcacgtg acccccgtcc gcgtgacctt
      901 ccaccggacg tttcactcgc tcgggcactc ggcgaacttc cgccggttct tcatcggcca
      961 ggcggtctcg gtgaccggca cgtggatgca gatggtggcc gccgcctggc tggtgctcca
     1021 gctcaccggc agcggggtgg cgcttggaat cgacaccgcg ctcgcgttcg ggccgatcct
     1081 gctgttcgga ccgctcgggg ggtcgctcgc cgaccgctac gacaagcgac gcatcctgct
     1141 cgcgacgcag gttaccttcg gcgcgctcgc gttgatgctc tgggggatcg tggcgaccgg
     1201 ggtcgtccag ctctggatgg tctatgccct ctcgttcctg cagggcgtca ccacctcgat
     1261 cgaccagccg gcccggcaga gcttcttcgc cgagatggtc cgggaccggg acctgccgaa
     1321 cgccatcagc ctcaacagcg cggtgatgac gggcaccagg atcgtcggac ccgcactcgc
     1381 gggcgtgctc atcgccggcg tcggcctgcc gtggtgcttc ttcatcaacg cctggtcgta
     1441 cctcgcggtg gtcgtcgcgc tgctctcgat gcacgccgat cagcttcgcg cccagcgggc
     1501 cccgcgtgag gccggcgcca tccgcgaggg gctccgctac gcgtggcgga cgggcgagct
     1561 ccggcggccg ctcctgctca tgtcggtgct ctttctgttc agcttcaact actcggtcct
     1621 gatgcccctg ttcgcgaagc gcacgttcgg cggcaacgcc gggacgctcg cactgctgtt
     1681 ctcggtggcc ggcgtcggct ccctcgcggg cgcgctcgtc atggccggcc gcccccgacc
     1741 cggcgaacgc cggctggcgc tcgcggctgt gggcgtgggt gtggtgacga tcctggtcgc
     1801 cctcgcgccg agcctggacg tcgcggtggt ctcgatgctg ccgctcggcg tggcgtccat
     1861 cgtgttcttc gtgacggcga actccacgtt gcagctgacc tcgcgtcccg acatgcgcgg
     1921 acgcgtcatg gcgctgtacg ggatcgtgtt cctggggacg acgccgttcg gcgcgccgat
     1981 ggcggggtgg gtcggggagc acctgggccc gcggatcgcg ttcgccggcg gtggcgtgat
     2041 cgcgctcgcg gccgggctgg tggggctgta cctcctgtcc cgccgggcgg ccgcagatcg
     2101 attcgcggtg gagccggtgg ccgccgcgga gaccgcctga gtcgtggtcg cggggaaccc
     2161 gtcgccggcg ggacccgccc gcgtcaggag cggagggcgt cgatgttcag ccgtacgtag
     2221 tccagctcga gccgcggcaa tcgggccgcc acggcgtgcg cgaacgcatc ggcctccgcg
     2281 tcggtcatcg accggacatg gtcgcgcagc acgaccgtgt gcaggaacgt ctcgagcgcc
     2341 tcgaggctct cgaacggggt gggctcggcg tgcagccagc actcggcgcg ggcgaacccg
     2401 gccgcccgca ggcgcttctc ggtgcgctcg ggcgtcgcga acaccttgcc ggtgaacggt
     2461 gctgcgccga gctcatcgag cacgcgcacc acgccggcga ggttcccgtc gccgccgcac
     2521 tgcgcgacga gccggccccc gggccgcagg accgccgcca ggttcgcgaa cagcgcgtcg
     2581 tgatcgggga cccagtggaa ggtcgcggtc gacagcacgg catcgacggg ttcggcgagc
     2641 ggcagcgggt ccagcagctc ggcgtgcacg aacgtgatcc ggtcgccgaa cggcgccagc
     2701 cgtcgacgtg ccgtccccag catcgcgctc gagccgtcca acgccacgac ccggccgcgg
     2761 gggagccgcc tggccaggag ctcggtcac
//
LOCUS       DATBRK010000398        14382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_229829, whole genome shotgun sequence.
ACCESSION   DATBRK010000398 DATBRK010000000
VERSION     DATBRK010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14382
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229829"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VIH70_11755"
     CDS             <1..286
                     /locus_tag="VIH70_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117081.1"
                     /translation="LTLIGALLGERWDRAEAIIRPFAWAIAGALVVGLVWFVWNRIRQ
                     IRREEAARVPRPPPPDVPGRAVPSTREPDADVSPLEGTTPAGIAEPVDET"
     gene            complement(307..795)
                     /locus_tag="VIH70_11760"
     CDS             complement(307..795)
                     /locus_tag="VIH70_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8117082.1"
                     /translation="MNIRPATHEDDGFLREMLYEAAAWNPDWPRERMIAALADPMLER
                     YHRDWGREGDAGVIAELDRQPVGAAWYRLFTAEEPGYGFVDEKTPELGIAVVPLHRRK
                     GIGETLLRALMVQAREEGYQALSLSVAVHNRSRMMYERAGFEPVREEAEGESWVMRAE
                     LG"
     gene            823..1611
                     /locus_tag="VIH70_11765"
     CDS             823..1611
                     /locus_tag="VIH70_11765"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY8117083.1"
                     /translation="MATLAQSRPLALLRAIGARSRVDDVSTHAAALAYQLFLSTLALS
                     LVGLALIGLAEDLLPFELPEGTGEQFANLTRTSATLGAVSLLALLWTASGLSRRASRA
                     LAVIFRTGPEGAVRGGVRALGTTLALIVLVGALPVVTGVVAAIRVSSGLEIPFRVFGF
                     AATAAMEFGLFLLAYTVLTPGSIGWRSHLPGALVMTTGWELFKLAGGLLLAYYVSRAR
                     LLYGTIGSVVGLLLFLRLASGLFVFGAEASAVLLDRGSGPSPTD"
     gene            1676..3304
                     /locus_tag="VIH70_11770"
     CDS             1676..3304
                     /locus_tag="VIH70_11770"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8117084.1"
                     /translation="MTQRLGTFRTVLADRNLRRVGLAFLGFNMTEFATWIAILVYAFE
                     RGGAAEMGAVSAILLVPSALVAPFAAYAGDRFRRDRVLAIDYAVQGLAMGATAAILYA
                     DAPAGLVYAAATIASISITFTRPAQNSLLPSLTPTPADLTAANVVFGVVEGVGKMLGP
                     IIAGILLGTSGPAAVFAVFAALTLLGALLVAKLRVDAGAVTPEARIDAADVFHETLRG
                     FHTLRRAAEPRLVVLLLSAGVIVVGALDVLFVATAIDLLGIGASGAGYLSAAFGAGAI
                     VGAASTVALVGRRRLTPSLAGGALLFGAPIGLVAVAPSAVGAPVLFATAGAGRSVGDV
                     SGRTLLQRISPNDVLSRVFGVLEGLMMLALVIGSVGAAVLIEAWGVETALVVVGAFLP
                     VVVLLSSGRLLSIDRSAEAPDAEILRLLRQIPFFAPLPAPAMERVVADVVARDAAAGD
                     VLIREGDRGDLFYVIVDGTVEITRGGEHVSEQGPGAYVGEIALLRDVPRTASVTAKTP
                     LRLLALEREPFLLAVTGHPVSHEAARAVATARSR"
     gene            complement(3301..4014)
                     /locus_tag="VIH70_11775"
     CDS             complement(3301..4014)
                     /locus_tag="VIH70_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HEY8117085.1"
                     /translation="MREAMGWALLGASSLIIGALVAFAVPIPRKVLGFILAFGSGVLT
                     SAVAYELVEEAVTVSANSYAVGLGFAGGALVFFVGDVAIDRWMKGSSRVGGLGIVLGA
                     VLDGVPESVVIGASLLTGETASAAVIVAVFLSNIPESISASSDLVRGGWSRWRVLLLW
                     VVVVVASTLAAGLGYVALEGASGSWIAAIQAFAAGAILTMLADEMMPEAFEYTDHTKT
                     VGLAVALGFALSAALSFAD"
     gene            complement(4021..4566)
                     /locus_tag="VIH70_11780"
     CDS             complement(4021..4566)
                     /locus_tag="VIH70_11780"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HEY8117086.1"
                     /translation="MDRDAAVALCREKCLKETTVRHLISVEGVMRALAGRFEQDEDLW
                     GLTGLFHDLDQDHTGDDAANHARLAAEWLREAAVDERVINGVLAHAYEGYRTDLMSRT
                     VVHADAVAGLLVAAALVRPERSAGMKVSSVKKKLKEKAFAPGVNRDEVTGVEASIGLP
                     LDEFLSISIDGLQSVAPEIGL"
     gene            complement(4566..6902)
                     /locus_tag="VIH70_11785"
     CDS             complement(4566..6902)
                     /locus_tag="VIH70_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8117087.1"
                     /translation="MTTQTRPGSSVLKRAFVGRAMASSRLEHTLLPKILALPVFSSDP
                     MSSVAYATEEIMIVLLAASASGKSYVFPISIAIGTLLAIVVISYRQTVRAYPSGGGAY
                     IVSKENIGTGAGLVAAAALLVDYVMTVVVSVVAGVYAITSAAPSLVGARVWLSVAFVL
                     FITLANLRGVRESGTLFAIPTYGFIAIVLVMIALGVVKCLGGCPAGDAIPPAPEVATT
                     AGTISLFVILHAFSSGSTALTGVEAISNGVPAFRRPQAKNAAETLAVMGVIAITMFLG
                     ISWLATHIEHVTVSEERTVIAQIGHTVFGGGIGFYTLSVFTAGILILAANTAYQDFPR
                     LSAILARDRFMPRQFMNRGDRLVFSNGVIGLAFFACVIIIAFNANLTQLIQLYVVGVF
                     TSFTLSQTGMVRHWLKEKHKGPAAQPGWRRSIVINTVGAITTGIVLVVITSTKFVHGA
                     WLSILAMALLVPTFLMIHRHYEQVKAQLRRGRVRPGDFGVNHMVLVIRDLDAAAAEAV
                     GVVRSIRPTELRVVHPCDGEIPPELQERWRSFSMGAPRLEPLVLGKDSILDALRRYVR
                     GIERSPDDFVNVVVPEVVSGSMLGYLLRAAGVVRLKAGLLREKNIVVTDVPVLVQDGQ
                     PVGVDALPLVPERTVVLAFVSGVNDATIRAANYADTLGASETRAIYFDLDPEAAHRMQ
                     EEWGAAGMRFPLDIVETPFRDLTGPMLDEVRRYTRRAGTLVLVVVPEFIVSKWRHLVL
                     HNQNALFVKRLFLFEPNVVLSSVPYVLGDDREPAAPAQ"
     gene            complement(6970..7647)
                     /locus_tag="VIH70_11790"
     CDS             complement(6970..7647)
                     /locus_tag="VIH70_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8117088.1"
                     /translation="MTRVLVVDDEPQIRRAIRTSLEAHGYEVRTVGTGEEAVVAAAED
                     DPELVLLDLGLPDLDGTDVIRRVRSFSEVPIIVLSVRDRQADKVAALDAGADDYVTKP
                     FGVQEVLARLRAALRRSQAGEPSPPVLHFGELEVDLGRRLVTVGSSPVHLTKTEYALL
                     EAFVTNPGKLLTHQWLLRKVWGPGYAQESHYLRVYVRSLRRKLGDEASAPRLILTEPG
                     VGYRWIG"
     gene            complement(7644..9119)
                     /locus_tag="VIH70_11795"
     CDS             complement(7644..9119)
                     /locus_tag="VIH70_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8117089.1"
                     /translation="MSERLMLVPGGGSRVRAVVVSLVAPALALGLALIVQPERELGAV
                     SLFLLAVVAASVAGGIWAGIGSSLVGFLALNYFFTEPLHTFRVHNRDDVVALAVFLVV
                     ALLVGWVVSRAVSDRDRATRREREARLLNLFATKALSGEPLERVLNDLAAALVDALRL
                     ASCSITARAAGRSYDVRRTRPDTIAGATVDVPIRSGEIAFGTLTASRAAPEEFVADDR
                     RLIEAAASQVAVYLERARLDGEIAAARLDVERSQARAALFSSVTHDLRTPLASIKTAV
                     TSLLQVDVRFDPAQARELLQTVLEETDRLNRLVGNILGLAQVRSGALEPVKEPTALDE
                     IVESVLHRLEPTLGGVNVRTIFRDAPDVPVDPVLIDQVLSNLLENAVRFSPPHGEVTI
                     SVAPWRSGVQVRVADQGPGIARADRERVFEAFTQLGAAAPDATGGSGLGLAISRAIVL
                     AHGGRIWIEGTPAGGTAVAFELPVRGAAPVPQEVPARDGRS"
     gene            9223..11652
                     /locus_tag="VIH70_11800"
     CDS             9223..11652
                     /locus_tag="VIH70_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8117090.1"
                     /translation="MAKTDAPTADPSATPVVAGAPAREEQLQPRGRGHLLKRLVLGAA
                     LPTAQLKHERLGKPTALAVFASDNLSSVAYATEEILKVAVPAVGVLAFSVVMPITFGI
                     LAVLAILLFSYRQTIKAYPSAGGAYIVTKDNFGLLTAQLAGVALLTDYVLTVSVSVAA
                     GVAAITSAAPGLYTYRVWLSVAFIWVIAWGNLRGVRESGRMFAVPTYFFITMMFLLLG
                     VGLAKSFAGDLHPLPIPADVAETTGAISLFLILHAFASGGAAVTGVEAISNGVPAFKP
                     PEWLNARTTLMWMGSLLGVMFLGLSFLAMKLQVAPTETKTVISEVGRAVFGSGALGTA
                     LFLGLQVGTTLILILAANTSFADFPRLANFHAKDNFMPRPLTKRGHRLVFSNGIIGLA
                     IASTALVVLFQADVHKLIPLYAIGVFTSFTLSQAGMARRHLRLREPGWRHGLFINGLG
                     AITTAVVTVVIAATKFADGAWAVMLFVPIMVWILVRMNHLYEGEHVELDEGLRPFEHE
                     VRQHPVAVLLVDDLDRKTMHALQYAKTIRSRATYALHVEEDPSKTEQLRRRWAELGIQ
                     VPLLVRRRRGDATKAIAAYASHLAVEGDVTVIVPAPSRMGRLERLRRGRTGARLARAL
                     AAHPRVRVTLVRDHPHDGGSEGSIRMLPRAAHLAVVLVDRPDRAAMQAVRYALTLGAD
                     EVTAVHAAVDPEMQDELIARWMDLRIPIALDLVECWDRDVARSVERYVVDLTGPRTEI
                     TVVLPRRDFARARQRLLHDRTSRSIARALGRYEHVDIAVVPYFLGRSGTRQGPKESVQ
                     DPTAASAGRAG"
     gene            11664..12323
                     /locus_tag="VIH70_11805"
     CDS             11664..12323
                     /locus_tag="VIH70_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY8117091.1"
                     /translation="MRVIIMGCGRVGILLTQELSKAGHEITVIDKNAAAFARLPPGFE
                     ARTIVGLGFDRDVLEDAGIKEADAFLAVSSGDNSNILSARVARETYHVPKVIARIYDP
                     MRADIYERLNIPTVSTTRWGVKQMILMLTHPGEEIKETLAAGDLFRMRVEIPTHLVGK
                     PVSSINLEGKILVAGVDRGGKGFIPVASSTFQEGDYAAVIVQKDALETLEELMQPIGD
                     H"
     gene            12325..13002
                     /locus_tag="VIH70_11810"
     CDS             12325..13002
                     /locus_tag="VIH70_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY8117092.1"
                     /translation="MRVVISGAGAVGRHLAADLANRGHSVTLIEQDPAVLEIAREWAP
                     QVDHMIGDACEPWVLEKAQLNNADVVVASTGDDEDNLVTALLAKQEFGVPRVLARVNH
                     PDNEWLFTEQWGVDAAVSPPHILTAMVEEEVTVGDLVRLLPLERGGISIVELRIPADS
                     PSAGRPLYELRLPPDATIVAIIREGHVVIPQPETVITDGDEVLALTVPDAESALRSAV
                     LGEGEGP"
     gene            complement(13043..13612)
                     /locus_tag="VIH70_11815"
     CDS             complement(13043..13612)
                     /locus_tag="VIH70_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117093.1"
                     /translation="MRRGQQHHAWKAWRNTIIAIAAVIAIGSLASANPSLRPPRGPHE
                     SIVIVPTGAGAPTGGTGHETKTADEGVSPSEGVDFAACEGLTGLDIAICRHEALLVVH
                     PDNQGLQNSLTHLLEAKAEHDAKADEDHGGHVPSEGGSSSSSASDHGQSGESHGSAGG
                     NSDAGGNGNGSAGGDGNGNAGGSGNGKDH"
     gene            complement(13675..14043)
                     /locus_tag="VIH70_11820"
     CDS             complement(13675..14043)
                     /locus_tag="VIH70_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY8117094.1"
                     /translation="MGLLDPFRRFLRRMAESDELRYAEEIRTWAESVPGSVRIAEAST
                     RSRVKLAGVVRRITVRPLEGHESLEAILSDGTGEVTVMWMGRRTIRGLSLGTRLVVEG
                     LLADQRGEKRMVNPSFEFRA"
     gene            complement(14082..>14382)
                     /locus_tag="VIH70_11825"
     CDS             complement(14082..>14382)
                     /locus_tag="VIH70_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY8117095.1"
                     /translation="EEGHYGGKGSESHKAAVIGDTVGDPFKDTAGPALNPLIKVMNLV
                     ALLFAPVIVEQTDNEVVRVVVVVLAVAVVAAAIWVSKSRRSELMVEASEPAVRAG"
BASE COUNT         2165 a         5122 c         4928 g         2167 t
ORIGIN      
        1 gctgaccttg atcggcgccc tgctcggcga gcggtgggac cgggccgagg cgatcatccg
       61 gccgttcgcc tgggcgatcg ccggggccct cgtggtcggg ctcgtgtggt tcgtgtggaa
      121 ccggatccgc cagatccggc gcgaggaggc ggcgagggtc cctcgtccgc cgccgccgga
      181 cgtccccggg cgggcggtcc cctcaacgcg cgaaccggac gcagacgtgt cgccgctcga
      241 ggggacgacg cccgccggca tcgcggagcc ggtcgacgag acctgagccc tcgaccgacg
      301 gcccgatcag ccgagctcgg cgcgcatcac ccaggactcg ccctcggcct cctcgcggac
      361 gggttcgaac cccgcgcgct cgtacatcat ccgcgaccgg ttgtgcaccg cgaccgagag
      421 cgagagcgcc tggtacccct cctcgcgcgc ctggaccatc agggctcgca ggagggtctc
      481 gccgatcccc ttgcgacggt ggagcgggac gaccgcgatg ccgagctccg gcgtcttctc
      541 gtcgacgaac ccgtaccccg gctcctcggc ggtgaacagc cggtaccagg ccgcgcccac
      601 cggttgccgg tcgagctcgg cgatgacccc ggcgtcgccc tcgcggcccc agtcacggtg
      661 gtaccgctcg agcatcgggt cggcgagcgc ggcgatcatc cgctcgcgcg gccaatcggg
      721 gttccacgcc gcggcctcgt acagcatctc gcgaaggaag ccgtcgtcct cgtgcgtcgc
      781 gggccggatg ttcacgaagc cccccgtgcc atgcttccgc ggatggccac cctagcgcag
      841 tcgcggccgc tggcgctgct gcgagccatc ggcgcgcgct cccgcgtgga cgacgtgagc
      901 acccacgcgg ccgcgctggc gtatcagctg ttcctgtcga ccctcgcgct ctcgctcgtc
      961 ggcctcgcgt tgatcggact cgcggaggac ctgctgccgt tcgagctccc ggaggggacg
     1021 ggggagcagt tcgccaacct cacccgcacg agcgccaccc tcggtgctgt cagcctgctt
     1081 gcgctcctgt ggacagcgtc ggggttgtcg cgtcgtgcga gccgtgcgct ggcggtcatc
     1141 ttccgcaccg gcccggaggg agcggtgcgg ggaggcgtcc gcgcgctcgg gacgacgctt
     1201 gccctcatcg tgctcgtcgg tgcgctgccc gtcgtcaccg gggtcgtcgc cgcgatccgg
     1261 gtctcgtcgg gcctcgagat cccgttccgc gtgttcgggt tcgcggcgac cgcggcgatg
     1321 gagttcggcc tgttcctcct cgcgtacacg gtgctcacgc ccggctcgat cggctggagg
     1381 tcgcatctgc cgggggcgct cgtgatgacc accgggtggg agctgttcaa gctcgcgggc
     1441 ggactgctcc tcgcctacta cgtctcgcgg gcgaggctgc tgtacgggac gatcggatcc
     1501 gtggtcgggc tcctgttgtt cctgcggctc gcgagcggcc tgttcgtgtt cggcgccgag
     1561 gcgtcggcgg tcctgctcga ccgcgggtcc ggtccgtcgc cgaccgactg agcccggaag
     1621 gcgagcccag tcggccgatt cgaccgcgcc gaacggcggt gcgaccatcc gaccgatgac
     1681 ccagcgcctc ggcaccttcc ggaccgtcct ggccgaccgc aacctgcgaa gggtcgggct
     1741 cgcgttcctc ggtttcaaca tgaccgagtt cgccacgtgg atcgcgatcc tggtctacgc
     1801 gttcgagcgc ggcggggccg ccgagatggg tgcggtctcg gccatcctgc tcgtgccgtc
     1861 ggccctcgtc gctccgttcg ccgcgtacgc gggcgatcgg ttccggcgcg accgggtgct
     1921 cgcgatcgac tacgcggtcc aggggctcgc gatgggggcg acggcagcca tcctgtacgc
     1981 cgacgccccg gccgggctcg tctacgcggc cgcgacgatc gcctccatca gcatcacctt
     2041 cacccgcccg gcacagaact cgctgctgcc gtcgctcacg ccgacaccag cggacctcac
     2101 cgccgcgaac gtcgtcttcg gcgtggtcga gggcgtcggg aagatgctgg gcccgatcat
     2161 cgcgggcatc ctgctgggga cctcgggacc ggcggccgtc ttcgcggtct tcgcggccct
     2221 gacgctgctc ggggcgctgc tcgtggcgaa gctgcgcgtg gacgccggag cggtcacgcc
     2281 cgaggcgcgg atcgacgccg ccgatgtctt ccacgagacg ctccgcgggt tccacaccct
     2341 ccgtcgagcc gccgagcctc gcctcgtcgt gttgctcctg tccgccggcg tcatcgtcgt
     2401 cggcgcgctc gacgtgctgt tcgtggcgac cgccatcgac ctgctcggga tcggtgcgag
     2461 cggggccggc tacctgagcg cggccttcgg cgccggcgcg atcgtggggg ccgcctcgac
     2521 ggtcgcgctc gtcggacgtc gccgcctcac cccgtcgctg gcaggcgggg ccctgttgtt
     2581 cggggcgccg atcggcctgg tcgccgtcgc cccgtcggcg gtcggcgccc cggtcttgtt
     2641 cgccacggcc ggcgccggtc gaagcgtcgg cgacgtttcg gggcggacct tgctgcagcg
     2701 gatctctccc aacgacgtcc tgtcgagggt cttcggcgtc ctcgagggcc tcatgatgct
     2761 cgccctggtc atcgggtccg taggcgccgc cgtcctgatc gaggcctggg gtgtcgagac
     2821 cgcgctcgtt gtggtcggag ccttcctgcc ggtcgtcgtc ctcctgtcgt cgggacgcct
     2881 gctctcgatc gaccgatccg ccgaggcgcc cgacgccgag atcctccggt tgctccggca
     2941 gatccccttc ttcgcgccgc tcccggcccc cgcgatggag cgggtggtgg ccgatgtcgt
     3001 cgcgcgggac gcggcggccg gcgacgtgct gatccgggag ggcgatcgcg gcgacctctt
     3061 ctacgtgatt gtcgatggca cggtcgagat cacgcgcggg ggcgagcacg tctccgagca
     3121 gggaccgggt gcgtacgtcg gcgagatcgc cctgctgcgc gacgtcccga ggacggcctc
     3181 ggtcaccgcg aagacgccgt tgcgcctcct cgcgctcgaa cgcgagccgt tcctgctcgc
     3241 cgtcactggc catcccgtga gccacgaggc ggcgcgcgcg gtggccacag ctcggtcgcg
     3301 ctagtccgcg aacgataggg cggccgacag cgcgaacccg agcgccaccg ccaaccccac
     3361 cgtcttggtg tggtccgtgt actcgaacgc ctccggcatc atctcgtccg cgagcatcgt
     3421 caggatcgcg ccggccgcga acgcctggat cgccgcgatc cacgagcccg aggcaccctc
     3481 gagcgccaca tacccgagac cggccgcgag cgtgctcgcc acgacgacga ccacccagag
     3541 cagcagcacc ctccaccgcg accatccccc gcggacgaga tcgctcgagg ccgagatcga
     3601 ctcggggatg ttggacagga acaccgccac gatcacggcc gcgctcgcgg tctcgccggt
     3661 gagcagtgag gcgccgatga cgacggactc ggggacgccg tcgaggacgg cacccagtac
     3721 gatccccagg ccgcccactc tcgacgaacc cttcatccat cgatcgatcg cgacgtcgcc
     3781 gacgaagaag acgagagccc cccctgcgaa gccgagcccg accgcgtacg agttggccga
     3841 gacggtgacc gcctcctcga cgagctcgta ggcgacggcg ctggtcagca cccccgaccc
     3901 gaacgccagg atgaacccca gcaccttccg agggatcggg accgcgaacg cgacgagcgc
     3961 gccgatgatc agcgacgagg cgccgaggag cgcccacccc atcgcctcgc gcacgtgccg
     4021 ctacagaccg atctcggggg ccactgactg caggccgtcg atcgagatcg agaggaactc
     4081 gtcgagcggg agcccgatgc ttgcctcgac gccggtgacc tcgtcccggt tcacgccggg
     4141 tgcgaacgcc ttctccttga gcttcttctt cacggaggag accttcatgc ccgcgctccg
     4201 ctccggccgg accagtgccg cggccaccag cagcccggcc acggcgtcgg cgtgcacgac
     4261 ggtcctggac atgaggtcgg tgcggtaccc ctcgtacgcg tgcgcgagca cgccgttgat
     4321 gacgcgttcg tcgacggcgg cctcgcggag ccactcggct gcgagccggg cgtggttggc
     4381 cgcgtcgtcg ccggtgtggt cctggtcgag gtcgtggaac aggccggtga ggccccacag
     4441 gtcctcgtcc tgctcgaagc gcccggcgag cgcgcgcatc acgccctcga ccgagatcag
     4501 gtgccggacc gtggtctcct tcaggcactt ctcgcggcag agcgcgacgg cggcatctcg
     4561 atccatcact gcgccggcgc cgccggctcc cggtcatccc ccagcacgta ggggacgctc
     4621 gacaggacca cgttcggctc gaagaggaac agtcgtttca cgaacaatgc gttctggttg
     4681 tgaaggacca gatgccgcca cttcgagacg atgaactcgg ggaccaccac caggacgagc
     4741 gtccccgcgc gcctggtgta ccgccggacc tcgtcgagca ttgggccggt gagatcacgg
     4801 aacggcgtct cgacgatgtc cagcgggaac cgcatcccgg ccgcgcccca ctcctcctgc
     4861 atgcggtgcg cggcttcggg gtcgaggtcg aagtagatag cccgcgtctc ggaggcaccc
     4921 agcgtatccg cgtagttggc ggcccggatg gttgcgtcgt tcacgcccga gacgaacgcg
     4981 agcacgacgg tccgttcggg taccagcgga agcgcatcga ccccgacggg ctgtccatcc
     5041 tgcaccagca cggggacatc ggtgacgacg atgttcttct cacggagcag ccccgccttc
     5101 agccgcacca cccccgccgc acgtagcagg tagccgagca tggatccgct caccacctcg
     5161 gggacgacga cgttcacgaa gtcgtcgggg ctgcgctcga tgccacggac gtagcgccgg
     5221 agcgcgtcca ggatgctgtc cttcccgagg acgagcggct ccagtcgggg ggctcccatc
     5281 gagaacgagc gccatcgctc ttggagctcg ggcgggatct cgccgtcgca cgggtggacg
     5341 acacggagct cggtgggtcg gatcgagcgg acgacaccga ccgcctcggc ggccgcggcg
     5401 tcgagatctc ggatcaccag caccatgtgg ttgaccccga agtcccccgg tcggaccctc
     5461 ccgcggcgga gctgtgcctt cacctgctcg tagtgccggt ggatcatcag gaaggtgggg
     5521 acgagcaggg ccatcgcgag gatcgacagc cacgccccgt ggacgaactt cgttgacgtg
     5581 atcacgacga ggacgatccc ggtcgtgatc gccccgaccg tgttgatcac gatcgaacgg
     5641 cgccagccgg gctgggcggc gggacccttg tgcttctcct tgagccagtg gcggaccatg
     5701 cccgtctgcg acaacgtgaa cgaggtgaac actccgacca cgtagagctg gatgagctgg
     5761 gtcaggttcg cgttgaacgc gatgatgatg acgcaggcga agaacgccag gccgatcacg
     5821 ccgttggaga agacgaggcg gtcgccccgg ttcatgaact ggcggggcat gaaccggtcg
     5881 cgggccaaga tcgccgacag ccgcgggaag tcctggtagg cggtgttcgc cgccaggatg
     5941 aggatcccgg cggtgaagac cgacaacgta tagaagccga tcccgccacc gaacaccgta
     6001 tgaccaatct gcgcgatcac ggtgcgctcc tcgctcaccg tgacgtgctc gatatgcgtc
     6061 gccagccagg agatccccag gaacatcgtg atcgcgatca cgcccatgac ggcgagagtc
     6121 tcggccgcgt tcttggcctg cgggcgccgg aacgccggga caccgttgga gatcgcctcc
     6181 acgccggtga gcgcggtcga gcccgaggag aacgcgtgca ggatcacgaa gagcgagatc
     6241 gttcccgccg tggtcgcgac ctcgggcgcg ggtgggatcg catcgcccgc gggacacccg
     6301 cccaggcact tgacgacgcc gagcgcgatc atcaccagaa cgatcgcgat gaacccgtag
     6361 gtagggatcg cgaacagggt gcccgattcc cgcacgccgc ggaggttcgc gagggtgatg
     6421 aacaggacga acgcgacgga gagccacacc cgagctccga cgaggctcgg cgccgcggac
     6481 gtgatggcgt agacgccggc gacgaccgag acgacgaccg tcatcacgta gtcgaccagc
     6541 agcgccgcgg ccgccaccag gccggccccc gtcccgatgt tctccttgct gacgatgtag
     6601 gcgccgccgc cgctcgggta ggcgcgaacg gtctgccgat acgagatcac cacgatggcc
     6661 agcaaggtcc cgatcgcgat cgagatcggg aacacgtagc tcttgccgct cgcgctcgct
     6721 gcgagcagga cgatcatgat ctcctccgtc gcgtaggcga cggaggacat cgggtcggag
     6781 ctgaagaccg gcagggccag gatcttgggg agcagggtgt gttccaggcg gctgctcgcc
     6841 atcgctcgcc cgacgaaggc tcgcttcagc accgagctcc cagggcgggt ctgggtggtc
     6901 acgggtcgag atgctacgac ccgaacccgg tcggcggggc gtggttcccc actcgatctc
     6961 gcggcgcgct cacccgatcc accggtagcc gacacccggt tcggtcagga tcagccgagg
     7021 cgccgaagcc tcgtccccca gcttgcggcg caacgaacgc acgtacacgc gcaggtagtg
     7081 actctcctgc gcataccccg gtccccacac tttccgcagg agccactggt gggtgagaag
     7141 tttcccggga ttcgtcacga acgcctcgag cagggcgtac tcggtcttcg tgaggtggac
     7201 gggcgagctc cccaccgtaa cgagtcggcg accgaggtcg acctcgagct cgccgaaatg
     7261 cagcaccggc ggtgagggtt cgccggcctg gctcctgcgc agcgccgcac gcagacgcgc
     7321 gaggacctcc tgcactccga agggcttggt gacgtagtcg tcggcccccg catcgagcgc
     7381 ggccaccttg tcggcctgtc ggtcccggac cgagagcacg atgatcggaa cctcggagaa
     7441 ggaccgaacg cgccggatca cgtccgtgcc gtcgaggtcg gggagaccga gatcgagcag
     7501 gacgagctcg ggatcgtcct ccgccgccgc aacgacggcc tcctccccgg tcccgacggt
     7561 gcgcacctcg tagccgtggg cctccaggct ggtccgaatc gcgcgccgga tctgcggctc
     7621 gtcgtccacg acgagcaccc gcgtcacgag cgtccgtccc gtgctggcac ctcctgcggc
     7681 acgggcgccg caccgcggac cggaagctcg aacgccaccg cggtgccgcc ggccggcgtc
     7741 ccctcgatcc agatgcggcc cccgtgcgcg agcacgatcg cacgagagat cgcgaggccg
     7801 agaccgctcc cgcccgtggc gtcgggggcc gccgcaccga gctgcgtgaa cgcctcgaac
     7861 acccgctcac ggtctgcgcg ggcgatgccg ggcccctgat cggccacgcg cacctgcacg
     7921 ccggaacgcc acggcgccac cgagatcgtg acctcgccgt gcggggggga gaagcggacg
     7981 gcgttctcga gcaggttcga aagcacctgg tcgatcagga ccgggtcgac cgggacgtcg
     8041 ggggcgtcgc ggaagatcgt ccggacgttg accccgccga gcgtgggctc caggcggtgg
     8101 agcacggact cgacgatctc gtccagcgcc gtgggctcct tcacgggttc gagcgctccc
     8161 gagcgcacct gggccaggcc caggatgttg ccgaccaagc ggttgagccg gtcggtctcc
     8221 tcgagcaccg tctgcaggag ctctcgcgcc tgcgcggggt cgaaccgaac gtccacctgc
     8281 aacaggctgg tcaccgcggt cttgatcgac gccagcggcg tgcggaggtc gtgggtcacc
     8341 gaggagaaca acgccgctcg cgcctggctc cgctccacgt ccagccgagc ggcggcgatc
     8401 tccccatcga ggcgggcacg ctccaggtag acggcgacct gactcgcggc ggcctcgatc
     8461 agccggcgat cgtcggccac gaactcctcg ggagccgcac gcgaggccgt gagggttccg
     8521 aacgcgatct cgccgctgcg gatcgggaca tcgaccgtcg cgccggcgat cgtgtccggc
     8581 cgcgtgcgac gaacgtcgta cgaccggccg gccgccctgg cggtgatgga acagcttgcc
     8641 aaccgaagcg catcgaccaa cgccgccgcg aggtcgttca gcacccgttc gagcggctcg
     8701 ccggacaacg ccttggtcgc gaacaggttg agcaggcgcg cctcccgttc gcgacgggtc
     8761 gcgcgatcgc ggtcggacac ggcgcgggaa accacccatc cgacgagcag cgctaccacg
     8821 aggaagaccg caagggcgac cacgtcgtcg cggttgtgca cgcggaaggt gtggagcggc
     8881 tccgtgaaga agtagttcag agccaggaag ccgaccaacg agctcccgat ccccgcccag
     8941 atccctccgg ccacggacgc ggccaccacg gcgagcagga agagcgagac ggcgccgagc
     9001 tcccgctcgg gctgcacgat cagggcgaga ccaagcgcga gggcgggagc cacgagtgag
     9061 accacgacgg ctcggacccg cgacccccca ccgggcacca gcatcaaccg ctcgctcacg
     9121 ccgccacgct acggcttcgc tacggcttct tcacggggaa gggcccggct ttcacgagcc
     9181 gtttacgcgt cacggacgac cctctgagtg gaaggaggca ccgtggctaa gacagacgct
     9241 ccaactgcag acccctcggc gacaccggtg gtggccgggg cgcctgcacg cgaagagcag
     9301 ctccagccgc gcgggcgagg gcacctgttg aagcgcctgg tcctcggggc cgcgctcccc
     9361 acggcgcagc tgaagcacga gcggctcggc aagcccaccg ccctcgcggt cttcgcgagc
     9421 gacaacctct cctcggtcgc ttacgcgacc gaggagatcc taaaggtcgc ggtcccggcg
     9481 gtcggggtcc tggccttttc ggtcgtgatg ccgatcacgt tcggcatcct ggccgtcctc
     9541 gcgatcctcc tgttctccta ccgccagacg atcaaggcgt acccgagcgc cggaggcgcc
     9601 tacatcgtca cgaaggacaa cttcggactc ctgacagcgc agctggccgg cgtcgccctg
     9661 ctgaccgact acgtccttac ggtctcggtc tcggtggcgg cgggcgtggc cgcgatcacc
     9721 tccgccgcac cggggctgta cacgtatcgg gtctggctgt ccgtcgcgtt catctgggtc
     9781 atcgcctggg gcaacctgcg cggagtgcgg gagtccgggc ggatgttcgc cgttccgacc
     9841 tacttcttca tcacgatgat gtttctgctc ctgggcgtcg gcctggccaa gtcgttcgcc
     9901 ggcgacctcc atccgctgcc catccccgcc gacgtggccg agaccacggg cgcgatcagc
     9961 ctcttcctga tcctgcacgc gttcgcctcc ggaggagcgg ccgtgacggg cgtcgaggcg
    10021 atctccaacg gggtccccgc cttcaaaccc cccgaatggc tgaatgcacg gacgacgctg
    10081 atgtggatgg ggagcctgct cggggtgatg ttcctcggcc tctcgttcct cgcgatgaag
    10141 ctccaggtcg caccgacgga gacaaagacc gtcatctccg aggtcggcag ggcggtgttc
    10201 gggagcggcg cgctggggac ggcgttgttc ctcgggctcc aggtcgggac gaccctcatc
    10261 ctgatcctgg cggccaacac ctccttcgcc gacttcccgc gcctcgcgaa cttccacgcg
    10321 aaggacaact tcatgccacg cccactgacc aagcgcggac atcgcctcgt cttctcgaac
    10381 ggaatcatcg gtctcgcgat cgcgtcgacg gcgctcgtcg tcctgttcca ggccgacgtg
    10441 cacaaactga tcccgctgta cgcgatcggc gtgttcacct cgttcacgct gtcgcaagca
    10501 ggcatggcca ggcgccacct gcgcctacgg gagcccggat ggcggcacgg cctgttcatc
    10561 aacggacttg gggcgatcac gacggccgtg gtcacggtcg tgatcgcggc aacgaagttc
    10621 gccgacggcg cctgggcggt gatgctgttc gtccccatca tggtctggat cctcgtgcgc
    10681 atgaaccacc tctacgaggg ggagcacgtg gagctcgacg agggcctccg gccgttcgag
    10741 cacgaggttc ggcaacaccc ggtggccgtg ctgctcgtcg acgacctcga ccggaagacc
    10801 atgcacgcgc tccagtacgc gaagacgatc cgttcgaggg ccacctacgc gctgcacgtc
    10861 gaggaggatc cctcgaagac cgagcagctc cgtcgtcggt gggccgagct cgggatccag
    10921 gttcccctgc tcgtccgccg gcgccgtggc gatgccacga aagcgatcgc cgcgtacgcg
    10981 tcccacctcg ccgtggaagg cgacgtcacg gtcatcgtgc cggcgccttc gcggatgggt
    11041 cgcctcgagc ggcttcgccg tggacggacg ggcgcccgcc tggctcgcgc gctcgccgca
    11101 catccgcggg tgcgcgtcac cctcgtccgc gaccatccac acgacggcgg cagcgagggt
    11161 tcgatcagga tgctgccacg agccgcgcac ctcgccgtcg tgctcgtgga ccggcccgac
    11221 cgcgccgcga tgcaggcggt tcggtacgcg ctcaccctgg gcgccgacga ggtcaccgcg
    11281 gtgcacgcgg ccgtcgatcc ggagatgcag gacgagctca tcgctcgctg gatggacctc
    11341 cgcattccga tcgcgctgga cctggtcgaa tgctgggacc gcgacgtcgc gaggtcggtc
    11401 gagcgatacg tggtcgacct cacgggacct cgcacggaga tcacggtcgt cctgccgcgc
    11461 cgggacttcg cccgggcacg tcagcgcctc ctgcacgacc ggacgagtag gagcatcgcc
    11521 cgtgcgctcg ggcgctacga gcacgtggac atcgccgtcg tcccctactt cctggggagg
    11581 tcgggcaccc ggcagggccc gaaggagtcg gtgcaggacc cgacggctgc gagtgctggt
    11641 cgagcaggct aaggtaacca gccgtgcgcg tgatcatcat gggctgtggc cgtgtcggca
    11701 tcctgctcac gcaggagctc tcgaaggccg ggcacgagat caccgtgatc gacaagaacg
    11761 ccgcggcctt cgcgcgactc ccccctgggt tcgaggcccg aacgatcgtg gggctcggat
    11821 tcgaccggga cgtgctcgaa gacgccggca tcaaggaggc ggatgcgttc ctggcggtct
    11881 cgagcggaga caactccaac atcctgtcgg cccgggtcgc acgcgagacc taccacgttc
    11941 ccaaggtgat cgcccggatc tacgacccga tgcgggcgga catctacgag cggctgaaca
    12001 tcccgacggt ctccaccacc cgatggggcg tgaagcagat gatcctcatg ctcacccacc
    12061 ccggcgagga gatcaaagag accctcgcgg ccggcgacct gtttcggatg cgcgtggaga
    12121 tccccacaca cctcgtcggc aagccggtct cctcgatcaa cctggagggc aagatcctcg
    12181 tcgccggcgt cgaccgcggc ggcaaggggt tcatcccggt cgcgtcgagc acgttccagg
    12241 agggcgacta cgcggccgtg atcgtgcaga aggacgcgct cgagacgctc gaggagctca
    12301 tgcaaccgat cggggatcac tgaggtgcgc gtcgtgatca gcggtgccgg cgcggtcggg
    12361 cggcacctcg cggccgatct ggccaaccgc ggtcacagcg tcacgctgat cgaacaggac
    12421 ccggcggtgc tcgagatcgc cagggagtgg gccccgcagg tcgatcacat gatcggggac
    12481 gcgtgcgaac cgtgggtgct cgagaaggct cagctcaaca acgccgacgt cgtcgttgcc
    12541 tcgaccgggg acgacgagga caacctcgtg accgcgctgc tcgcgaagca ggagttcggc
    12601 gtccctcggg tgctcgcgcg cgtgaaccac cccgacaacg aatggctgtt cacggagcaa
    12661 tggggcgtgg acgcagcggt ctcgccgccg cacatcctca cggcgatggt ggaggaggag
    12721 gtcacggtcg gcgacctcgt ccgtctgctg ccgctcgaac gcggtggaat ctcgatcgtc
    12781 gagctcagga tccccgccga ttcaccgtcg gccgggcgcc cgctgtacga gctgcggctg
    12841 ccacccgacg ccacgatcgt ggcgatcatc cgcgagggac acgtcgtgat cccgcagccg
    12901 gagaccgtga tcaccgacgg cgacgaggtg ctcgcgctca ccgtgccgga cgcggagagc
    12961 gcgctccggt cggccgtgct tggagagggc gaggggccct aggcaacact cgaaggggct
    13021 cgaagaaaca ggttcaacgg gctcagtggt ccttgccgtt gccgctgccg ccggcgttgc
    13081 cattgccgtc tccgccggcg ctgccattgc cgtttccgcc ggcgtcgcta ttgccgccgg
    13141 cgctcccgtg ggactccccg ctctgaccgt gatcgctggc cgaggacgac gaggagccgc
    13201 cctcggacgg aacgtggcca ccgtgatcct cgtcggcctt cgcatcgtgc tcggccttgg
    13261 cctccagcag gtgcgtgagc gagttctgca ggccctggtt gtccgggtgg acgacgagca
    13321 gcgcctcgtg gcggcagatg gcgatgtcga gcccggtcag gccctcgcag gccgcaaagt
    13381 cgacgccttc cgacggcgac acgccctcgt cggcggtctt ggtctcgtgg ccggtccctc
    13441 cggtcggagc accggccccc gtggggacga tgacgatgga ctcatgggga ccccggggcg
    13501 gccgcaggga cgggttggcc gacgcgaggg aaccgatcgc gatcaccgcg gcgatcgcga
    13561 tgatcgtgtt ccgccaggcc ttccacgcat ggtgctgttg accccgccgc atgtaagagg
    13621 tatcggcggg cggcctcgtg gaacttgacg ggccgctcga cctccccagg tcgcctacgc
    13681 acggaactcg aagctcgggt tcaccatccg cttctcgccg cgctggtcgg cgagcaggcc
    13741 ctcgaccacg agacgagtgc cgaggctcag cccgcggatc gtccgacggc ccatccacat
    13801 caccgtgacc tctccggtcc cgtcggacag gatcgcctcg agcgactcgt ggccctcgag
    13861 cggccggacc gtgatccgtc gaaccacgcc ggcgagcttg acgcgcgagc gtgtggacgc
    13921 ctcggcgatc cgaacgctgc cggggacgct ctccgcccac gtccggatct cctccgcgta
    13981 gcggagctcg tcgctctccg ccatccggcg gaggaaccgc cggaacggat cgaggagtcc
    14041 catcggcgtg gccgtccctc cgttcccgag atgcggggag cctatcccgc gcgcaccgcg
    14101 ggctcggacg cctcgaccat gagctcggac ctgcgggact tggagaccca gatggcggcc
    14161 gcgaccaccg cgaccgcgag cacgacgacg acgacgcgga ccacctcgtt gtcggtctgc
    14221 tcgacgatca ccggcgcgaa cagcagcgcg accaggttca tcaccttgat gagcgggttg
    14281 agcgccggcc cggccgtgtc cttgaacgga tcgccgaccg tgtcgccgat caccgcggcc
    14341 ttgtgcgact cgctgccctt gcccccgtag tggccctcct cg
//
LOCUS       DATBRK010000399         9026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1385045, whole genome shotgun sequence.
ACCESSION   DATBRK010000399 DATBRK010000000
VERSION     DATBRK010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385045"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VIH70_11830"
     CDS             complement(<1..460)
                     /locus_tag="VIH70_11830"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY8117096.1"
                     /translation="MSWFPIAIALVLGLAFGSLMAVAIYRVPAGESLVRPRSRCPDCG
                     APIRIADNIPVISWLMLRGRCRACGARISPVYPLTELASGGLFVAVALVYEDPWRTVL
                     LAPFAGLMVAISVIDIRHRRIPNRLVYPAFVIAAAVIIVGGLAGGGLDALD"
     gene            complement(496..2049)
                     /locus_tag="VIH70_11835"
     CDS             complement(496..2049)
                     /locus_tag="VIH70_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117097.1"
                     /translation="MLAHIRDDERGVAMVVAVMVVFVVMLLATVVFAQAVHNSTASGY
                     DRKRLQSVDAAEAGLNYFFNYLEQTPPCASQPSGSCSSASLSARCGTVTAGICASNPL
                     AVPQPIGVDVEPGTAAFTITPTWYDAAGNLFNGAISNVSYPRAVRVVSEGTTNGTTPR
                     RMETYLTIAPTFGGFAGAVVTNTSLNLVNSFTISGNSINDGDIYVTTGNASMTSGNQV
                     IRGNVYVPFGSLSVSAQAHIYGTAWARDSVTINHPQALIDQAAISSTSSLTVSNGNVS
                     GVGSYCTTVSGTSRIQGGTVAQCQGPPPTPSFPRLTYDDAVAPNADPAWRANCGSSGT
                     LDCYYLKQFTDCTTARAYVEGTGAGSFGGGAGVPSGYSGVVVRILATCTYSPSNNVTV
                     SVGKDLAIITDGTLNFSQQSTWNATGGTNKVFLIRPWPADSNLGFCSSYGNITVGNNT
                     NFGATLNVGLYTPCTATMSNQNAFFGQVVGGTVVIGNNWSMNYRPIVIPGALVAGFTE
                     DVVYIREVA"
     gene            complement(2059..2769)
                     /locus_tag="VIH70_11840"
     CDS             complement(2059..2769)
                     /locus_tag="VIH70_11840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8117098.1"
                     /translation="MRPRRPRERRLPRALASLRRLRRTDEGMTLVELVVAMSILGVVL
                     LVFTSVLASVQRGVVRQDSLSQTLDSARLAIQQLDRELRSGNVLYDPALENAPVGTPG
                     RIASCTGCLPGYTLRVYTQTNADTRGPTPYRCVLWKIEDQQLMTRWWPPLEPTSASGW
                     RIVAEGIVNRDLSPATVAFVLDPDPLKGGRTLNVVYAANADLEHHPTQTAKVQASLTG
                     RNTSYGYPANVCQSTPTG"
     gene            complement(2747..4579)
                     /locus_tag="VIH70_11845"
     CDS             complement(2747..4579)
                     /locus_tag="VIH70_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117099.1"
                     /translation="MRSDERGFSLLETVIAITVIFGALLTLSYAATIGFRYESLARQK
                     QTATGIADQIMEQTRGLAWDRITAGHLTSDLAGDPNLVTSCSGDAAGVYRLLSCTAQT
                     GRPGSGEVVVAGASSCASGSPDCVYPLVRHTGQITQNNIAFTWSTYDTNSCPTVTSAG
                     CAATTPYRVTVIVTWTGGAAAPNKIVQVQSLFWSPTGCRSTSTHPFAAPCQPFFLGVS
                     SVAQGNINIAGTVAGTTFESGDLSTSGVESSVQQEQLSQAEGSFRQSGVRLVDSLGAE
                     TAAGDIAASSAADTDPGTGASTWSEVTCGTTVVCAGGSVSTGSSNTVVLTAPSGETAR
                     SDSTTAAGAANVCPPPPDTAQTDGKPCSGSSIQQGGTLSAVMTLNGAATPLGSVTLAR
                     ILAAAGSPNKTFSDRVQYSATGLCSPITGSDGCLEQTTTRAVGTVNVGGLPANVLTTI
                     PWAGLNAWNGYYFSIVGYTDSATAAVGTNSTTSTAGGNVPAPTASIGVGGTVYCWNGL
                     GYTSVPASSATAVACAPFDFTWPNGGHLVRIQMSGTTTPAVITRSPQAAATSQTDVTA
                     QITPPGATITYVVSVDGATVADLTIAVNLNTLEARGTYAAAPTP"
     gene            complement(4839..5306)
                     /locus_tag="VIH70_11850"
     CDS             complement(4839..5306)
                     /locus_tag="VIH70_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8117100.1"
                     /translation="MLERFWQRRKEDEGFTLIELMVVVLIIGILIAIALPTFLGARTR
                     AQDRAAQSDLRNGIAAAKTYFTDGDVYTGFDTTKAEAIEPSLVWTAAGTPTAGQTVIQ
                     AVAASSVVLTALSGSGTYFCIADNSATGGGTFYNSSTTYASVDTLAECNLAKW"
     gene            complement(5508..6719)
                     /locus_tag="VIH70_11855"
     CDS             complement(5508..6719)
                     /locus_tag="VIH70_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8117101.1"
                     /translation="MPATFDYKVRDKTGALVSGKLVGDSEALVMRRLREMGLTPIRVK
                     QASTGLKMEIHLRPGRVKLKQVAVFCRQFATMVNSGLPILRALSILTDQTESKELSKI
                     LFAVRASVENGSSLSAAMAEHPKAFNNLFISMVKAGETGGVLDDVLLSLADQIEREVE
                     LRRQIKSAMTYPIVVVALVTLILAAMLLFVVPQFETIYSSLGGTLPLPTRMLLGVSRA
                     VRSYWYVVVLGAVVGSFLLRRYKKTDAGRARVDAVKIRIPIFGPLFHKVALARFASTL
                     GMLLRSGVPILQALDNVTETVNNRVIGDAVDDVKTSVREGESIAKPLGRHKVFPPMVV
                     QMMAVGEETGAVDTMLDKVAEFYNSEVTGTVDALTSLIEPLLIAVIGGAVGAAVIALY
                     MPMFNVINLLQ"
     gene            complement(6743..7903)
                     /locus_tag="VIH70_11860"
     CDS             complement(6743..7903)
                     /locus_tag="VIH70_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY8117102.1"
                     /translation="MSPEGREDEVQIPIPELLEAMVERGASDLHITVGTPPTIRQFGD
                     LVRLDGYPVLDKRGIQGMIYAILPQKMRERLEQELELDMSYSLPGRARFRVNVYYQRD
                     SLGAVFRLIPYEIRTVEELDLPSVVADLARSPRGFVVVTGPTGSGKSTTLASMVDIVN
                     RERQAHIMTVEDPIEFLHKHNSCVVNQREVGADTQSFAQALKHVLRQDPDVILVGEMR
                     DLASMSTAITAAETGHLVFATLHTQDAPQTIDRIIDMFPPHQQQQVRVQLATTLQGVV
                     TQQLLQTVDGRSRVVAVEVLICTPAVRNLIREGKVHQIYSIMQAGGRFGMQTMDQSLA
                     DLVKSGKVSQQLAYERCHDAEELKRLIGTGGGGFDGGYGAPAAAGVVLGGNY"
     gene            complement(7904..>9026)
                     /locus_tag="VIH70_11865"
     CDS             complement(7904..>9026)
                     /locus_tag="VIH70_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY8117103.1"
                     /translation="QAAADRASDVHIEPTETDVRVRFRVDGVLHEVMHSPKSIQGGLI
                     SRLKVMGDLNIAEKRIPQDGRVSLRINNRSLDLRLATLPTVHGEKIVIRILDKSNALL
                     QLSELGFLPQAFESYERSFRKPYGAILVTGPTGSGKSTTLYATLNIVNSIDRHIVTVE
                     DPVEYRLPGVNQMQVNPKAGLTFASALRSILRADPDIILIGEIRDRETAMIAVESALT
                     GHLVLSSLHTNDAPSAITRLIEMQVETFLVGSAIDCVVAQRLARKLCEKCKQAFAPEE
                     SELIEAGFAESRIPDLGLFFRASGCQSCSNTGYRGRIGLYEVMPMTEEIERLTVERAS
                     SETVKRVAIEQGMYTLRDDGLYKAASGLTSIEEIARVVK"
BASE COUNT         1460 a         2994 c         3030 g         1542 t
ORIGIN      
        1 cgtccagggc gtccagtcca ccaccggcca gaccgccgac gatgatgacc gcggccgcga
       61 tcacgaacgc cgggtagacc agacggttcg ggatgcggcg gtgacggatg tcgatcacgg
      121 agatggcaac catgagcccg gcgaagggag cgaggaggac cgttcgccac gggtcctcgt
      181 acacgagcgc gaccgccacg aagaggcctc cgctggccag ctcggtgagt gggtagacgg
      241 gcgagatccg agcaccgcac gcacggcacc ggcccctcag catcagccat gagatcacgg
      301 ggatgttgtc ggcgatgcgg atcggcgcgc cgcagtccgg acaccgagaa cgaggacgca
      361 cgagcgactc ccccgcgggc acccggtaga tcgccacggc catgaggctc ccgaacgcca
      421 accccagcac gagggcgatc gcgataggaa accagctcat gacggcgaac cctccctcct
      481 cgacggcatc gacggctacg cgacctcgcg gatgtacaca acatcctcgg tgaaaccggc
      541 cacgagagcg ccggggatca cgatcggtct gtagttcatg gaccagttgt tcccgatcac
      601 cacggtgccg cccaccacct ggccgaagaa cgcgttctgg ttgctcatcg tggccgtgca
      661 cggcgtgtac aggccgacgt tgagcgtggc gccgaagttc gtgttgttcc ccaccgtgat
      721 gttgccgtag ctcgagcaga agccgagatt gctgtcggcg ggccagggac ggatcaggaa
      781 caccttgttg gtcccacccg tcgcgttcca ggtggactgc tgtgagaagt tcagggtccc
      841 gtcggtgatg atcgccagat ccttgcccac gctgacggtc acgttgttgc tgggcgaata
      901 cgtgcaggta gcgaggatgc gaacgaccac gccgctgtag ccgctcggca cgcccgcccc
      961 accgccgaag ctccccgcac cggtcccctc cacgtaggcg cgggcggtcg tgcagtcggt
     1021 gaactgtttc aggtagtagc agtcgagagt tccactcgaa ccgcagttcg cccgccaggc
     1081 ggggtccgcg ttcggggcga cggcgtcgtc gtaggtgagg cgcgggaacg acggggtcgg
     1141 cggtggccct tgacactgag cgaccgtccc tccctggatc ctcgatgtcc ccgacaccgt
     1201 cgtgcaatag gagccgaccc cgctgacgtt gccgttcgag acggtgagcg aggaggtcga
     1261 ggagatcgcg gcctgatcga tcaatgcctg cgggtgattg atcgtcaccg aatccctcgc
     1321 ccatgccgtg ccgtagatgt gggcctgcgc cgagacgctc agcgaaccga atgggacgta
     1381 gacgttgccc ctgatgacct ggttccccga tgtcatcgag gcgttcccgg tcgttacgta
     1441 gatgtctccg tcgttgatgc tattgccgct gatcgtgaag ctgttcacga ggttgagcga
     1501 cgtgttcgtc acgaccgcac ccgcgaaacc gccgaacgtg ggtgcgatgg tcaggtacgt
     1561 ctccatcctc cgcggggtcg tcccgttggt cgtgccttcc gagacgaccc tgacggcccg
     1621 cggatacgag acgttggaga tcgcgccgtt gaagaggttg ccggcggcgt cgtaccacgt
     1681 aggtgtgatc gtgaaggcgg cggtccccgg ctctacgtcg acgccgatgg gctgcggcac
     1741 cgcgagcggg ttggaggcgc agatgcccgc cgtgacggtc ccacaccgtg ccgaaaggga
     1801 tgccgaactg caactgccgc tcggctggga agcgcacgga ggtgtctgct ccaggtagtt
     1861 gaagaagtag ttgaggcccg cctccgccgc gtcgaccgac tgcagacgct tgcggtcgta
     1921 cccgctcgcc gtgctgttgt ggacggcctg cgcgaacacg accgtggcca ggagcatcac
     1981 gacgaacacc accatgacgg cgaccaccat ggcgacccct cgctcgtcgt ctcggatgtg
     2041 ggccagcatc ggatcctcct agccggtggg tgtcgattgg cagacgttgg cgggatatcc
     2101 gtacgacgtg ttgcggcccg tgagggacgc ctgaaccttc gcggtctggg tcgggtggtg
     2161 ctcgagatcg gcgttggccg cgtacacgac gttgagcgtt cggccgccct tgagcggatc
     2221 ggggtccagg acgaaggcca ccgtcgccgg gctcagatcg cgattcacga tgccctccgc
     2281 gactatccgc catccgctcg cggaagtcgg ctccaggggg ggccaccatc gagtcatgag
     2341 ctgttggtcc tcgatcttcc acagaacgca ccggtacggt gtcgggcccc gtgtgtcggc
     2401 gttcgtctgc gtgtagacgc gcagggtgta cccgggcaga cacccggtgc acgaggcgat
     2461 ccttcccggc gtcccgacgg gggcgttctc gagcgctggg tcgtacagga cgttgcccga
     2521 gcgcagttcg cgatccagtt gctggatggc gagccgggcg ctgtcgaggg tctggctgag
     2581 cgagtcctgc cggaccacgc cgcgctgcac cgaggcaagc acggaggtga acaccaacag
     2641 cacgacgccc aggatggaca tggcgacgac cagttcgacg agcgtcatgc cctcgtcggt
     2701 ccggcgaagg cgccgcaggg aagcgagcgc tcgagggagc ctccgttcac ggggtcggcg
     2761 cggccgcata cgttcccctt gcctcgagcg tgttcaggtt cacggcgatg gtcagatccg
     2821 cgaccgtggc cccatccacc gacaccacgt aggtgatggt ggctccgggg ggcgtgatct
     2881 gcgccgtcac gtcggtctgc gacgtggcgg ccgcctgggg cgaccgggtg atcaccgccg
     2941 gggtggtcgt ccccgacatc tggatccgga ccaggtgacc gccgttcggc cacgtgaagt
     3001 cgaagggcgc gcaggccacg gccgtcgccg aggacgccgg caccgatgtg tagccgagcc
     3061 cgttccagca gtagacggta ccgcccacgc cgatggacgc cgtcggggcc ggcacgttgc
     3121 cgccggccgt cgaggtggtg ctgttcgttc cgaccgcagc cgtcgcgctg tccgtgtagc
     3181 ccacgatcga gaagtagtag ccgttccacg cgttcagacc cgcccacggg atggtggtca
     3241 gaacgttcgc ggggagtccg ccgacgttca cggtgccaac ggcgcgggtc gtggtctgct
     3301 cgaggcatcc gtcggagccc gtgatcggcg agcacagacc ggtcgcgctg tactggactc
     3361 ggtcggagaa cgtcttgttc ggacttcctg cggccgcgag gatgcgtgcg agcgtgacgc
     3421 tgccgagcgg tgtcgccgct ccgttcagcg tcatgaccgc cgagagcgtg ccgccttgct
     3481 ggatgcttga ccccgagcat ggcttgccgt ccgtctgagc cgtgtccggc ggcggaggac
     3541 agacattcgc ggcgccagcc gccgtcgtgc tgtcgcttcg ggccgtctcg ccgctcgggg
     3601 cggtcagcac cacggtgttc gacgagccgg tcgagaccga cccgcccgcg cacacgaccg
     3661 tcgtcccaca ggtcacctcg gaccaggtgc tcgcccccgt ccccggatcc gtgtccgcgg
     3721 cggacgacgc cgcgatgtct ccggccgccg tttccgctcc gagggagtcc accagacgaa
     3781 cccccgactg acggaagctg ccctcggcct gcgacagctg ctcctgctgc acgctggatt
     3841 ccacgcccga cgtggagaga tcgccgctct cgaaggtggt cccggcaacg gttcccgcga
     3901 tgttgatgtt gccctgtgcg acgctcgaga caccgaggaa gaacggctga cacggtgcgg
     3961 cgaacgggtg ggtcgatgtg cttcggcacc cggtgggcga ccagaacagg ctctggacct
     4021 gcacgatctt gttcggagcc gcggccccac cggtccacgt cacgatcacg gtcacgcgat
     4081 agggcgttgt cgcggcgcag cccgcgctcg tgacggtggg gcagctgttc gtgtcgtagg
     4141 tgctccaggt gaacgcgatg ttgttctgcg tgatctgccc cgtgtggcgg accagcgggt
     4201 acacgcagtc gggcgagccg ctggcacacg aggacgcgcc ggcgacaacc acctcgccgg
     4261 agcccggcct cccggtctgc gccgtgcagg acaggagtcg atagacgccg gcggcatcac
     4321 cggagcacga ggtcacgagg ttcgggtctc ccgcgaggtc agaagtgaga tgcccggccg
     4381 tgatccgatc ccacgcgagt ccccgcgtct gctccatgat ctgatcggcg atcccggtcg
     4441 cggtttgctt ctgacgtgcc agcgactcgt accggaaccc gatcgtcgcc gcgtaggaga
     4501 gcgtcagcag ggcaccgaag atcaccgtga tggcgatgac ggtctcgagc aggctgaagc
     4561 cgcgctcgtc ggagcgcaaa cggcagagga tgcctcgcgc ccccgggcgg cgatgccaga
     4621 acggattccc tgtagtcatc gatggccttc ttgtaggggt gtcggccatt cggtggagtc
     4681 gcttgaggcg ccgccggacg cctaccagcc cttatcgcag ccggcggtga actccacgtc
     4741 ggcccagtcg ccctgcgtcg atcggtcgcc gaacggagat cgaacgtcga agggggcggc
     4801 tccacggccg cccccttctt tgcattacct tggttcgcct accacttcgc cagattgcac
     4861 tccgccagag tgtcgacgct ggcgtacgtg gtgctggagt tgtagaacgt cccgccgccc
     4921 gtcgccgagt tgtcggcgat gcagaagtac gtccccgaac cgctgagcgc ggtcaagacg
     4981 acactgcttg cggccacggc ctggatcacg gtctgccccg ccgtcggcgt ccccgcagcg
     5041 gtccacacga gggacggttc gatggcctct gccttcgtcg tgtcgaagcc cgtgtacacg
     5101 tcgccgtccg tgaagtaggt cttggcggcg gcgatgccgt tccgcaagtc cgactgcgcg
     5161 gctcggtcct gggccctggt cctggcgccg aggaaggtcg gcagagcgat ggcgatcagg
     5221 atgccgatga tcagaacgac caccatcaac tcgatgaggg tgaagccctc gtcctccttc
     5281 cgacgctgcc agaaacgctc gagcatggag tcacctccgc ttcttgattg catgcgcgtc
     5341 ccgatgacgc gcatctacct ggggtttcgg tcgcatggag gggcggctta accttccaga
     5401 aggcgtctgg gggggggaat gactcgaacg aactagtcac ttggaaccgg gccgccgcga
     5461 acgtcgacga atgccgcgtt ccgccgtcgt cgatccgacc ttctcgacta ctgcagcagg
     5521 ttgatcacgt tgaacatcgg catgtacagg gcgatgacgg ccgcgcccac cgcaccgccg
     5581 atcacggcga tcaggagcgg ctcgatcagc gacgtcaggg cgtcgacggt tcccgtcacc
     5641 tcgctgttgt agaactcggc gaccttgtcg agcatcgtgt cgacggcgcc cgtctcctcg
     5701 ccgaccgcca tcatctggac gaccatgggc gggaagacct tgtgccgccc gagcggcttg
     5761 gcgatcgact cgccctcccg aacgctggtc ttcacgtcgt cgaccgcgtc cccgatcaca
     5821 cggttgttca ccgtttcggt cacgttgtcg agtgcctgga ggatcggcac gccggaccgg
     5881 agaagcatcc cgagcgtcga ggcgaatcgg gccaacgcga ccttgtggaa caggggaccg
     5941 aagatcggga tcctgatctt caccgcgtcg acgcgagccc gtccggcgtc ggtcttcttg
     6001 tacctgcgga gcaggaagga ccccaccacc gccccgagca cgacgacgta ccagtagctg
     6061 cgcaccgccc tggagacgcc cagcagcatc ctggtcggaa ggggaagcgt gcccccgagg
     6121 ctcgaataga tcgtctcgaa ctgcgggacc acgaagagca gcatcgcggc gaggatcagc
     6181 gtcacgaggg ccacgacgac gatcgggtac gtcatcgccg acttgatctg acggcgcagc
     6241 tcgacctccc tctcgatctg atcggcgagg gacaggagca cgtcgtcgag gacgccaccg
     6301 gtctcgccgg ccttgaccat cgagatgaac aggttgttga acgccttggg atgctcggcc
     6361 atcgcggccg agagcgagga gccgttctcg acgctcgccc ggaccgcgaa caggatcttg
     6421 gagagctcct tgctctcggt ctggtcggtg aggatcgaga gcgctcgcag gatcggcagt
     6481 cccgaattca ccatcgtcgc gaactgcctg cagaacaccg cgacctgctt cagcttgacg
     6541 cgacccggcc gcagatggat ctccatcttg agaccggtgg aggcctgctt cacgcggatg
     6601 ggcgttaggc ccatctcgcg gagcctcctc atcacgagcg cctcgctgtc gcctaccagc
     6661 ttccccgaga cgagggcgcc ggtcttgtcg cgaaccttgt aatcgaacgt tgccggcatc
     6721 gctccgtcct acccgctctc gatcagtagt tcccgccgag caccacaccg gccgcggcgg
     6781 gcgcaccgta gccaccgtcg aaaccgccgc caccggtgcc gatcaggcgc ttcagctctt
     6841 cggcgtcgtg acagcgttcg tacgcgagct gctggctcac cttcccgctc ttgaccaggt
     6901 ccgcgagtga ctggtccatc gtctgcatcc cgaaacgacc accggcctgc atgatcgagt
     6961 agatctggtg caccttcccc tcccggatga ggttccggac cgcgggcgtg cagatgagaa
     7021 cctcgaccgc caccaccctc gaacgcccgt ccacggtctg cagcagctgc tgggtcacga
     7081 ccccctggag ggtcgtggcg agctgcaccc gcacctgctg ctgctgatgc ggcgggaaca
     7141 tgtcgatgat gcggtcgatc gtctgcggcg cgtcttgggt gtggagcgtg gcgaagacca
     7201 ggtgtccggt ctcggcggca gtgatcgcgg tcgacatcga cgcgaggtca cgcatctctc
     7261 cgacgaggat gacgtcgggg tcctgacgca ggacgtgctt gagggcctgc gcgaacgact
     7321 gcgtgtcggc cccgacctcc cgctggttca ccacgcagct gttgtgcttg tggaggaact
     7381 cgatcgggtc ctcgacggtc atgatgtgtg cctgccgctc gcggttcacg atgtcgacca
     7441 tcgaggccag cgtcgtcgac ttgcccgaac cggtcggccc cgtgacgacg acgaacccgc
     7501 gcggcgacct cgcgaggtcg gccacgacgc tcggcaggtc cagctcctcc acggtgcgga
     7561 tctcgtacgg gatcaggcgg aacacggccc ccagggaatc gcgctggtag tagacgttca
     7621 cgcggaagcg cgcacgtccg gggagcgagt acgacatatc gagctcgagc tcctgctcga
     7681 gacgctcgcg catcttctgc ggcaggatcg cgtagatcat gccctggatg ccgcgcttgt
     7741 cgaggacggg gtacccgtcc aggcgaacga gatcgccgaa ctgacggatg gtcggcgggg
     7801 ttcccaccgt gatgtggagg tccgaggccc cgcgttccac catcgcttcg agcagctcgg
     7861 ggatcgggat ctggacctcg tcctctcgcc cctccggact caatcacttc accacccttg
     7921 cgatctcttc gatggaggtc agacccgacg cggccttgta gagcccgtcg tcgcggagcg
     7981 tgtacatccc ctgctcgatc gcgacccgct tcacggtctc cgacgacgcg cgctcgacgg
     8041 tgaggcgttc gatctcctcg gtcatgggca tgacctcgta caggccgatc cgccctcggt
     8101 acccggtgtt cgagcacgat tggcacccgg acgctcggaa gaacaatccg aggtcgggga
     8161 tccgggactc ggcgaacccc gcctcgatga gctcggactc ctcgggcgcg aacgcctgct
     8221 tgcacttctc gcagagcttg cgcgcgagcc gctgggcgac cacgcagtcg atggccgagc
     8281 ccaccaggaa cgtctcgacc tgcatctcga tcaaccgcgt gatcgccgac ggcgcgtcgt
     8341 tcgtgtggag cgaggagagt acgaggtgac ccgtgagcgc ggattcgacc gcgatcatcg
     8401 ccgtctcccg gtcgcggatc tcaccgatca ggatgatgtc ggggtccgcg cgaaggatcg
     8461 atcggagagc ggacgcgaac gtgagccccg ccttcgggtt cacctgcatc tggttgacgc
     8521 cggggagccg gtactcgacc ggatcctcga cggtcacgat gtgccgatcg atgctgttca
     8581 cgatgttgag cgtcgcgtac agcgtcgtcg acttgcccga cccggtgggg cccgtcacga
     8641 ggatcgcgcc gtacggcttc cggaacgaac gctcgtagct ctcgaacgcc tgggggagga
     8701 agccgagctc cgagagctgg agcagggcgt tgctcttgtc caggatcctg atcacgatct
     8761 tctcgccgtg gacggtcggc agggtggcca gccgcaggtc caggctccga ttgttgatcc
     8821 gcagcgagac gcgcccgtcc tgcgggatgc gcttctcggc gatgttcagg tcgcccatga
     8881 ccttcaggcg gctgatcagc ccgccctgga tgctcttggg cgagtgcatg acctcgtgca
     8941 ggacgccgtc cacgcggaac cgcacacgga cgtcggtctc cgtcggctcg atgtgcacgt
     9001 ccgacgctcg gtcggccgcg gcctgc
//
LOCUS       DATBRK010000400         6121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_520745, whole genome shotgun sequence.
ACCESSION   DATBRK010000400 DATBRK010000000
VERSION     DATBRK010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520745"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VIH70_11870"
     CDS             complement(<1..250)
                     /locus_tag="VIH70_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8117104.1"
                     /translation="MSADQMTADRPFSLVLAGVGGSERSVEAARQAARLASAARAQLI
                     VAYVIEHRPYEEGDAERALDAASAAARAAGADPEARVVT"
     gene            331..777
                     /locus_tag="VIH70_11875"
     CDS             331..777
                     /locus_tag="VIH70_11875"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY8117105.1"
                     /translation="MLRGDETSEVSVVGPGTRIEGTVVAAGSLRVEGEVKGKITAERE
                     VTLSPQGRVEANIQAGSIVLAGQVKGNLAAKGNVSLPADSRLDGNIRAHNVDVGGVVM
                     GDIVGQGRVALGPRARVEGQITSTTLAIADGAVFIGSSTMDEQTPK"
     gene            complement(794..1054)
                     /locus_tag="VIH70_11880"
     CDS             complement(794..1054)
                     /locus_tag="VIH70_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8117106.1"
                     /translation="MTPQALTCREVIDLLTDYVEDALPPEERRRVEAHLATCDGCTAY
                     LEQVRETIRLTGMLTEEQVPDEDKQRLLAAFRDWTRSGSPGG"
     gene            complement(1051..1710)
                     /locus_tag="VIH70_11885"
     CDS             complement(1051..1710)
                     /locus_tag="VIH70_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8117107.1"
                     /translation="MSAPGVVASREDLELVERLRAGDELAFMMLVEQHQAAMLRIARM
                     YVSSRAVAEDVVAEAWLGVVRGIDRFEGRSSIRTWIYRIVANIAKTRGAAEGRVVPFS
                     ALAGDDETDPGVESSWFYDANGRNPGGWTTFPEDWRGIPEDRLVGNETLRHIGRAIQT
                     LPPLQAEVIRLRDVLGWSADEVRNALDLSEVNQRVLLHRARTRVRRELDAYLRTGWED
                     A"
     gene            1765..3804
                     /locus_tag="VIH70_11890"
     CDS             1765..3804
                     /locus_tag="VIH70_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY8117108.1"
                     /translation="MGDDREPIALGIDIGGTFTDLAAVDRRDGSLILAKVATTPSLLQ
                     QGVLSALERSELDPDDVGAFVHGSTVVINAVTERRGARVALVTTEGFRDVLEIGRANR
                     PDLYNLSYRKPVPFVPRRLRFEVRERISHTGEALVPLDEPGLESVAERLLALDVEAVA
                     ICFLHAWTNPAHERRAAELLSKLLRVEVVASHEVSAQWREYERTSTTVLSAYVKPTVA
                     SYLHELGSGLRAAGVDAPLYAMRSSGGVSTFERAVEAPITLLESGPVAGVTAAAELAR
                     RLGVEDVLSLDIGGTTAKTSTVRGGRVPIETLHHIERTPTSAGYPVQAPVVQIVEIGA
                     GGGSIAWVDEAGGLHVGPRSAGAEPGPACYGRGGDEPTLTDANLIAGRLDPDYFLGGA
                     MPLDTDAAHRGLARLGERMGVDAETAARGVLRYVVAQMSHALRLVTLRRGHDPRDFTF
                     VAFGGAGPLHAALLARELGVARTVIPPAPGHFSALGMLLGDVRADAVRTHVGALDPVA
                     IDPLFDELEAEAASELEREAGKHRTERFARLRYVGQEHTLDVALGEGPIDGDLLTRLR
                     ERFDAASEETYAFRLTTPVELVEARVSVSAARDEPIEWLAEPTPAPELRPRDVDLDEH
                     GGVQRADVVERRSLEAGARLAGPCIVEEPATTVLVLPGQSVTVDELSNLVIEEAS"
     gene            3801..>6121
                     /locus_tag="VIH70_11895"
     CDS             3801..>6121
                     /locus_tag="VIH70_11895"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY8117109.1"
                     /translation="MTDRFTMDVLREAFFAVTDEMFESLKRTSQSPVIYEVLDFAVGL
                     TDARGELVSQGNGIAGFLGPLGEATRDTLVRVPDLVPGDVVATNDPFAGGGGHLSDVA
                     MVRPIFVDGGLVAFAAAKGHWTEVGGKDPGSWTADSVEIFQEGLQLPFVRVVRAGELD
                     RDLVAVIGANSRLPDMTIGDLTAFAACLEVAERRVLELSQRYGVDDVTSAMAAAHERS
                     EALSRAAVERLPRGVFEAEDLIDEDGLGNGPFPIRVRVEIDGERVVADFTGTHPQVPG
                     PVNCTRSGLVSGIRTVFKAITDPNEPGSDAWFRPFEVVCPPGTIFTARRPAPVALYFE
                     ATEAATDLVWKALAPAMPELLTAGSFVSVCATAIACTHPDTGEPALLVEPQAGGWGAS
                     VLKDGEHGLVSVGDGETYVIPAEVCEQRYGIRVERFGFDVVEAGAGRRRGGRGLVREY
                     RMLTDGSLTVGFGRHLSPPWGLDGGHEGSPNYVGVVRADGSRERFGKVARYPLREGDL
                     VRLVTGTGGGAGNPRERERELVREDLEDGIVTEREARDVYAAVPVDGGARQHPTAMLD
                     LLRAAPLIDGHNDLLWELRQGRDPADPTLMTDLPRMAAGGVGGQFWSVYVPSDLPADR
                     AVTMTFEQIEALLDIVRTNPDRLELARSADDVERIAAAGRVASMIGVEGGHAVGGSLG
                     TLRVLAEFGMGYLTLTHNDDTRWADSATGEHSHGGLTRFGEEVVREVNRCGWLVDLSH
                     TSDDTMRQAIEVSAAPVIFSHSSARALCDVPRNV"
BASE COUNT          859 a         2160 c         2207 g          895 t
ORIGIN      
        1 tggtcacgac ccgcgcctcg gggtcggcgc cggcggcgcg cgcggcggcg ctcgcggcat
       61 cgagggcgcg ttccgcgtcc ccctcctcgt acgggcggtg ctcgatgacg taggcgacga
      121 tcagctgcgc gcgagcggcc gaggcgaggc gtgcggcctg gcgcgcggcc tcgacggaac
      181 gttccgaccc gccgacgccg gcgaggacga gagagaacgg ccgatcggcg gtcatctggt
      241 cggcagacac gacggcattg tcgccggggc cgtcccgccg gggcaaccat cgcgttcggc
      301 ttatacgctt gcggtccaag gaggtgctgc atgttgcgtg gagacgagac ctccgaggtc
      361 tcggtggtcg gtccgggaac tcggatcgag ggcaccgtcg tggccgccgg ctccctcagg
      421 gtcgagggag aggtcaaggg aaagatcacc gccgaacggg aggtgacgct ctccccccag
      481 ggtcgggtgg aggcgaacat acaggccggg agcatcgtcc tcgccgggca ggtcaagggc
      541 aacctcgccg ccaagggcaa cgtgtccctg ccggccgaca gccggctcga cggcaacatc
      601 cgcgcccaca acgtcgacgt gggaggggtg gtcatgggcg acatcgtcgg ccagggcagg
      661 gtggcgctcg gcccacgcgc tcgcgtcgag ggtcaaatca cttcgacgac gctggcgatc
      721 gccgacggag ccgtcttcat cgggagctcc accatggacg agcagacgcc caagtagggc
      781 tcagctcgcg ccgtcatccc ccggggctgc cggaccgggt ccagtcccgg aacgcggcga
      841 ggagccgttg cttgtcctcg tcggggacct gctcctcggt cagcatccct gtcagccgga
      901 tcgtctcccg cacctgctcg aggtaggccg tgcacccgtc gcaggtcgcg aggtgcgcct
      961 cgacccggcg acgctcctcc ggcgggaggg cgtcctcgac gtagtcggtg agcaggtcga
     1021 tcacctcgcg acaggtcagg gcctgcgggg tcatgcgtcc tcccatcccg tcctcaggta
     1081 ggcgtccaac tcccggcgca cgcgcgttcg cgctcgatgc aggagcaccc gctgattgac
     1141 ctccgagagg tccagggcgt tgcgcacttc gtcggcgctc cacccgagga cgtcacgaag
     1201 ccggatcacc tccgcctgca acggcggcag ggtctggatc gcccgcccga tgtggcgaag
     1261 ggtctcgttg cccacgagcc ggtcttcggg gatccctcgc cagtcctcgg gaaaggtcgt
     1321 ccatccgccc gggttgcggc cgttcgcgtc gtagaaccac gaggactcga cccccggatc
     1381 cgtctcgtcg tcgccggcga gcgcggagaa ggggacgacg cggccctcgg ccgctcccct
     1441 cgtcttggcg atgttcgcga cgatccggta gatccacgtt cggatcgacg agcgtccctc
     1501 gaagcggtcg atgcccctga ccacaccgag ccaggcctcc gccacgacgt cctcggcgac
     1561 ggctcgggac gacacgtaca tccgcgcgat ccgcagcatg gccgcctggt gctgctcgac
     1621 gagcatcatg aacgcgagct cgtcgccggc ccgaaggcgt tcgacgagct cgaggtcctc
     1681 ccgggaggcc acgacgccgg gggcgctcac gacgtgaagg gtacgaccgt gcgccgaggc
     1741 gcggccgtga cgtataacgc cgggatggga gacgaccgcg agcccatcgc gctcgggatc
     1801 gacatcggcg gcaccttcac ggacctggcc gcggtcgacc ggagggacgg ttcgctgatc
     1861 ctggcgaagg tcgccacgac gccgtcgctc ctccagcagg gcgtcctgtc ggcgctcgag
     1921 cgctccgagc tcgacccgga cgacgtggga gcgttcgttc acggcagcac cgtcgtgatc
     1981 aacgccgtca ccgaacggcg cggagcccga gtggcgttgg tgacgacgga ggggttccgc
     2041 gacgtgctcg agatcggccg cgcgaaccgc cccgacctgt acaacctctc gtaccgcaag
     2101 ccggtgccgt tcgtgccacg tcgactgcgg ttcgaggtgc gcgagcggat ctcgcacacg
     2161 ggcgaggcgc tcgtgccgct cgacgagccc ggtctcgagt ccgtcgccga acggctgctc
     2221 gcgctcgacg tcgaggcggt cgcgatctgc ttcctccacg cgtggacgaa cccggcgcac
     2281 gagcgccggg ccgccgagct cctgtcgaag ctcctgcgcg tcgaggtcgt ggcctcgcac
     2341 gaggtgagcg ctcagtggcg cgagtacgag cgaacgagca cgaccgtcct ttccgcatac
     2401 gtgaagccga cggtcgcgtc ttatctgcac gagctcggct cgggactccg agccgccggc
     2461 gtcgacgcac ctctgtacgc gatgcggtcg agcggcggcg tctcgacgtt cgagcgagcg
     2521 gtcgaggcgc ccatcacgct gctggagagc ggcccggtcg ccggggtgac cgccgcggcg
     2581 gagctcgccc ggcggctggg ggtcgaagac gtgctgagcc tcgacatcgg cggcacgacc
     2641 gcgaagacgt cgaccgtgcg tggtggacgc gtgccgatcg agacgctgca tcacatcgag
     2701 cggaccccga cgagcgccgg ctatccggtc caggctccgg tcgtccagat cgtcgagatc
     2761 ggggccgggg gcggctcgat cgcctgggtg gacgaggcgg gcggtctcca cgtcggtcct
     2821 cggagcgcgg gcgccgagcc cggtccggcg tgctacggcc gcggcggcga cgagcccacc
     2881 ctcaccgatg ccaacctcat cgccggacgt ctcgatcccg attacttcct cggcggcgcg
     2941 atgccgctcg acaccgacgc ggcgcatcga ggcctggctc ggctgggcga gcggatgggg
     3001 gtggatgccg agacggcggc ccgaggcgtc cttcgctacg tcgtcgcgca gatgtcgcac
     3061 gcgctccgcc tcgtcacgct gcggcgcggt cacgaccctc gcgacttcac gttcgtggcg
     3121 ttcggcggcg cggggccgct gcacgccgcg ctgctcgcgc gagagctcgg cgttgcgcgg
     3181 acggtgatcc ctcctgcccc gggccacttc tccgcgctcg ggatgctgct cggcgacgtg
     3241 cgggcggacg cagttcgaac acatgtcggt gccctcgatc cggtggcgat cgaccccttg
     3301 ttcgacgagc tcgaggcgga ggccgcgtcc gagctcgaac gcgaggccgg caaacaccgc
     3361 accgaacggt tcgcccgact ccgatacgtc ggccaggagc acacgctcga cgtggcgctc
     3421 ggcgaagggc cgatcgacgg cgacctcctc acccgattgc gggagcgctt cgatgccgcc
     3481 tccgaggaga cgtacgcctt ccgtctgacc acacccgtcg agctcgtcga ggctcgcgtc
     3541 agcgtctcgg cggcgcgcga cgagcccatc gagtggctgg ccgaacccac gccggctccc
     3601 gagctccggc cccgggacgt cgacctcgac gagcacggag gcgtgcagcg agcagatgtg
     3661 gtggaacgtc gatcgctcga ggccggcgct cgtctcgccg gtccctgcat cgtcgaagag
     3721 cccgccacca ccgtgctcgt cctgcccgga cagtcggtga ccgtggacga gctctcgaac
     3781 ctcgtgatcg aggaggcgtc gtgaccgacc ggttcacgat ggacgtcctt cgcgaggcgt
     3841 tcttcgccgt gaccgacgag atgttcgagt ccctcaagcg aacgtcgcag agccccgtga
     3901 tctacgaggt cctggatttc gccgtcgggc tcaccgacgc ccgcggcgag ctcgtctcgc
     3961 agggcaacgg catcgcgggg ttcctcgggc cgctcggcga ggcaacgcgc gacacgctcg
     4021 tacgggttcc ggacctcgtc cccggcgacg tcgtcgcgac gaacgacccc ttcgccggcg
     4081 gcggcggcca tctgtccgac gtcgcgatgg tccggccgat cttcgtggac ggcgggctcg
     4141 tcgcgttcgc ggcggcgaag ggtcactgga cagaggtcgg tggcaaggac cccgggtcgt
     4201 ggaccgccga ctcggtggag atcttccagg aggggctgca gctgccgttc gtccgcgtcg
     4261 ttcgcgccgg cgagctcgac cgcgacctcg tcgccgtgat cggcgcgaac agccgcctgc
     4321 cggacatgac gatcggcgat ctgactgcgt tcgcggcctg tctcgaggtc gccgagcgcc
     4381 gggtgctcga gctcagccaa cggtacggcg tcgacgacgt cacgtcggcg atggccgccg
     4441 cgcacgaacg gagcgaggcg ctttcgaggg ccgccgtcga gcgtcttccc cgcggcgtgt
     4501 tcgaggccga ggacctcatc gacgaggacg gcctgggcaa cggcccgttc ccgatccgcg
     4561 tgcgcgtcga gatcgacggc gagcgcgtcg tcgcggactt cacgggcacc catccccagg
     4621 tgccggggcc cgtgaactgc acgcggagcg ggctcgtctc cgggatccga accgtgttca
     4681 aggcgatcac ggatcccaac gaacccggct ccgacgcgtg gttccggccc ttcgaggtgg
     4741 tctgcccgcc cggcacgatc ttcacggccc ggcggccggc gcccgtcgcc ctgtacttcg
     4801 aggcgaccga ggccgcgacc gatctcgtct ggaaggcgct cgcgccggcg atgccggagc
     4861 tcctcacggc gggcagcttc gtctccgtct gcgcgacggc gatcgcgtgt acacacccgg
     4921 acaccggcga acccgcgctg ctcgtcgagc cgcaggcggg cggctggggc gcgagcgtgc
     4981 tcaaggacgg tgagcacggc ctcgtctcgg tcggagacgg cgagacgtac gtcatcccgg
     5041 ccgaggtgtg cgagcagcgg tacgggatcc gggtcgagcg gttcgggttc gacgtggtcg
     5101 aggccggggc gggccggcga cgcggcgggc gcggcctggt gcgcgagtac cgcatgttga
     5161 ccgacggcag cctcaccgtc ggcttcggcc gtcacctgtc tcccccctgg ggcctcgacg
     5221 gcggccacga aggctcgccg aactacgtcg gggtcgtgcg cgcggacggc tctcgtgagc
     5281 ggttcggcaa ggtcgcgcgc tacccgctgc gggagggtga cctcgtccgg ctcgtcaccg
     5341 gaacgggcgg cggcgccggt aacccccgcg agcgcgaacg cgaactcgtt cgcgaagatc
     5401 tcgaggacgg catcgtgacc gaacgcgagg cgcgcgacgt gtacgccgct gtgcctgtcg
     5461 atgggggggc ccgccagcat ccgaccgcca tgcttgatct ccttcgagcc gctcccctga
     5521 tcgacggcca caacgacctg ttgtgggagc ttcggcaggg ccgcgatccg gccgacccga
     5581 ccctcatgac cgacctgccg cgcatggccg caggcggcgt aggcggccag ttttggtcgg
     5641 tgtacgtgcc ctcggacctt ccggcggatc gcgccgtgac catgacgttc gagcagatcg
     5701 aggcgctcct cgacattgtc cgcacaaacc ccgaccggct cgagctcgcc cggtcggccg
     5761 acgacgtcga gcggatcgcc gccgccggac gggtcgcgtc gatgatcggt gtcgagggcg
     5821 gccacgcggt cggcggctcg ctcgggacgc tccgggtcct ggccgagttc ggcatgggct
     5881 acctgacgct cacgcacaac gacgacacgc ggtgggccga ctcggccacc ggcgagcact
     5941 cgcacggcgg gctgaccagg ttcggggagg aggtcgtccg cgaggtgaac cggtgcggct
     6001 ggctggtgga cctcagccac acctcggacg acacgatgcg ccaggcgatc gaggtgagcg
     6061 cggcgccggt gatcttctcc cactcctcgg cccgggccct gtgcgacgtg ccgaggaacg
     6121 t
//
LOCUS       DATBRK010000401         5607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_946832, whole genome shotgun sequence.
ACCESSION   DATBRK010000401 DATBRK010000000
VERSION     DATBRK010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946832"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VIH70_11900"
     CDS             complement(<1..207)
                     /locus_tag="VIH70_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003563948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8117110.1"
                     /translation="MTLQRMDNVGIVVESLDAAISFFAELGLELEGRAMIEGDWSGRV
                     TGLRDQRVEIAMMRTPDGHGRIELS"
     gene            complement(769..1167)
                     /locus_tag="VIH70_11905"
     CDS             complement(769..1167)
                     /locus_tag="VIH70_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117111.1"
                     /translation="MFSIRSMTGLVAGALALSLVLVPAASAVPQDPNSTPVSQDCEGS
                     TGVRIFLHPGAGKALWEVTAEATPDPNYLIKVLEHDEYVNGQYIGHFTYSFGNKTGLG
                     DTFRCTFYETFTDPDGNFIEVFGTSYKVAV"
     gene            1669..>5607
                     /locus_tag="VIH70_11910"
     CDS             1669..>5607
                     /locus_tag="VIH70_11910"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY8117112.1"
                     /translation="MRFGVLGPLEVAGEDGLVPLGGPKQRIVLAHLVLGANKVVSAEH
                     LIDALWGEGLPEDPKSTLQVYVSRLRSALGGDAVEGQARGYVLRAARDDVDALRFEDL
                     LAAARGNGSDPRETDKILTEALELWRGPALADLSGEPSLTGEIARLEELRLQVLEETN
                     DARLEFGRHVQVVAELETLTRTHPLRERLWRELMLALYRSDRQAEALAAFERARTVLA
                     EELGIDPSRDLQLLHERILRQDPELDLKGEPLRGYRLLEQVGEGAFGVVYRATQPQIG
                     REVAIKAVAPELANHPDFIRRFEREAQIVAKLEHPHIVPLYDYWREPDAAYLVMRFLR
                     GGSVEDLLRSGPLDPQRASSILDQVAAALSAAHRQGIVHRDVKPGNVLLDELGNAYLT
                     DFGVALEAGSPERSSGTMIRGTPGYLSPEQVRLDPASPRSDIYALGIVAYEMLTGAPP
                     FPESSLTALLDHHVRDSIPSVQEVRPDLPAALDRVIGRATAKDAEDRFSDALELASAV
                     RAALQGGAPTVTVLAGEIRNPYKGLRAFLEADAADFFGREGVTKRLLRRLEEDRPGSR
                     FLAVVGPSGSGKSSVVRAGLVPALRRGSLPGSERWYVIDMLPGAHPLRELESALLGLA
                     VAPPPSLLDELDGDTRGLLRAVDRILPDPDAELLIVLDQLEEVFTLIEDEDERFHILE
                     SLRAATTEAGSRVRVVATLRADFFDQPLSVRGFGDLLAQRTEAVTPMSPEELERAIVA
                     PAERAGLAVEPRLLAAMIADVVDRPGALPLLQFTLTELAERREDGVLTLVGYRRLGGV
                     SGALARRAEQMFEGMNEGARDACRQVFLRLVTLGEGSEDTRRRVRRSELQPLTDARTL
                     DGVLDAFGRHRLLSFDRDPATREPTVEIAHEALLGVWDRLHGWIDEARDDVRTQRRLA
                     AAVSEWESAERDPSYLLRGVQLDQAHEWARTTTLVLSDADRAYLAEGVEVQRAVSERE
                     RALERRSMRRLRSLMALGVAAALVATTLTVVAVSQRRRAEGEARVARARELAAAATAN
                     LSVDPERSILLALAAVGATWDVDRTVVPQAEEALHRALQESRVVLTVPQGREIAVSAD
                     GTRFATTGQDGTAIVWEMDTGKRLLTLRGHEGEVNGVAFSPDGSLLATTGDDRTVRLW
                     DAASGRQVHVLRGHRDLVLGVAFSPDGSLLATSSQDGTVRIWDVAAGTQHLVLRGPPG
                     EKFNNFSGLTPAFSPDGSRVASGGWFSTPIWDLATGKISMLLARQPVEAFAVAFSPDG
                     TRVATAVHRDAQTWDAETGEPLTAFSGHTGDILAVAYSPDGTRIATGADDG"
BASE COUNT          839 a         1934 c         1914 g          920 t
ORIGIN      
        1 cgagagttcg atccggccgt ggccgtccgg cgtacgcatc atggcgatct cgacgcgctg
       61 gtcgcgcagt ccggtgacgc gccctgacca gtctccttcg atcatggctc gcccttcgag
      121 ttcgaggccc agctcggcga aaaacgagat agcggcatca agcgattcca ccacgatgcc
      181 gacgttgtcc atccgttgaa gcgtcacgcg gcccactcta gtcctgttgc cacttcgaca
      241 cgcccaccgg acgagcgcac cggctgacgg gtgcgccgtc ggcacgatgg cttcgttgtt
      301 cgttcctctc ccgctgacct gcgcgagcgt cctctagcct gagcgtactt cgacctgagg
      361 aggaccagcg ccctgacctg gagctgtgcg cttgtagtcc caacgggatt tgaacccgtg
      421 tctccacctt gagagtcggc gtctccacct gcgatgacgt gaggatccgc ctcgtcacgt
      481 gcggattcga tgctccagtc gttcccgtca tttctaaacg tttcccgggc gctcgcggga
      541 cgaatgcggg acgaccgtcg cgctcgatag agccaggatc gcgatgccgg tgaccgctac
      601 gcaaggactg gccctgtttg agagccggga tttcggcgcc agttggacgc cgcaacgatg
      661 cccgtggagg gagcgcaacc gcgcaggacc tcacacgcac ctcggcccgg ctgacgcgga
      721 gaggccggtc cgcggaccga cctctccccc cgaccggtaa gacgccgact acaccgccac
      781 cttgtaactc gtcccgaaga cctcgatgaa gtttccgtcg gggtcggtga aggtctcgta
      841 gaacgtgcat ctgaacgtgt ccccgaggcc ggtcttgttg ccgaagctgt acgtgaagtg
      901 accgatgtac tggccgttca cgtattcgtc gtgttcgagg accttgatca gatagttcgg
      961 atcgggggtc gcctccgccg tgacctccca gagggccttg ccggcacccg ggtgaaggaa
     1021 gatcctcacc ccggttgagc cctcgcagtc ctgcgagacg ggcgtcgagt tcgggtcctg
     1081 cgggacggcg gaggcggcag ggaccaggac gagtgagagc gcgagcgccc ccgccaccaa
     1141 gcccgtcatc gaacggatcg agaacatgcg ccgaggcgtg cggcgctcac gagtagcacg
     1201 atccatctgc attcccctct ctgatcgtcg gtaccacatt gcatcgaccg actacgcgca
     1261 aggcggtggg tcggtacctg gacttgcgaa cgagaacgcc ccggcggatg cgacgagcgc
     1321 cacgatcccg gtcgcgaaga gcatgcgtac ctgttgacgc ctcatagcgt cctctttggt
     1381 ccttcgtctc agcggtccgt tggtgtcagc ggttcgatcg tgcggttcac tggttcccac
     1441 ctccttcgcc ccctcgctat cccgcggtcc cacgaggtaa ggtgcgccgc ctcgctttca
     1501 atccgccttc agatcgcctt cggtcgcctt tcagggttgg ggtcgagcat ccgcagccac
     1561 ccctgaaacc ggattgaaag gacagctcga caggctcccc tcgtgatcga acgcgccacg
     1621 tctcgccgag tggtgtcgcg cacaggcggg cgggagtacc gtcggcccat gcgtttcggc
     1681 gtcctgggac cactcgaggt agccggggag gacggcctcg tccccctcgg gggtccgaag
     1741 cagcggatcg tcctcgctca cctggtcctg ggcgccaaca aggtcgtgtc cgccgagcac
     1801 ctgatcgacg cgctgtgggg agaggggctg ccggaggacc cgaagtcgac tctgcaggtg
     1861 tacgtctcac ggctccgatc cgcgctcggg ggcgatgccg tcgaggggca ggcgcgcggt
     1921 tatgtcctgc gggccgctcg tgatgatgtg gacgccctac gattcgagga cctgctggcc
     1981 gcagcacgag ggaacggctc cgacccgcgc gagaccgaca agatcctgac cgaagccctc
     2041 gagctctggc gcggccccgc cctcgccgac ctgagcggcg agccctccct tacgggcgag
     2101 atcgcgcgcc tggaggagct ccgtctgcag gtgctcgagg agacgaacga cgctcgcctc
     2161 gagttcggcc gtcacgttca ggtcgttgcg gagctcgaaa ccctgacgcg gacccatcca
     2221 ctccgcgaac ggctgtggcg cgagctgatg ctcgccctgt accgatcgga ccgacaggcg
     2281 gaggccttgg cggcgttcga gcgggcgcgg accgtgctgg cggaggagct cggcatcgat
     2341 ccgtctcgcg atctccagct cctgcacgag cggatccttc gccaggaccc tgagctcgat
     2401 ctcaagggcg aaccgctgcg gggctatcgg ctcctggagc aggtcggcga gggtgcgttc
     2461 ggcgtggtct accgcgccac ccagccgcag atcggacgcg aggtcgcgat caaggcggtc
     2521 gcgcccgagc tcgcgaacca tccggacttc attcgtcgct tcgagcgcga ggcgcagatc
     2581 gtcgccaagc tcgagcaccc gcacatcgtc ccgctctacg actactggcg agagccggat
     2641 gccgcctatc tcgtcatgcg gttccttcga ggcggcagcg tcgaggacct gcttcggtcg
     2701 ggaccgctcg acccccagcg agcgtcttcg atcctggacc aggtggccgc ggcgctctcg
     2761 gcggcgcatc ggcagggcat cgtgcaccgg gacgtgaagc ccgggaacgt cctgctcgac
     2821 gagttgggca acgcctacct cacggacttc ggggtcgcgc tcgaggcggg atcacccgag
     2881 cgttcgtccg ggacgatgat ccgcggcacc ccaggctatc tctcgcccga gcaggtcagg
     2941 ctcgatcccg cgtctcctcg atccgacatc tatgcgctcg ggatcgtggc gtacgagatg
     3001 ctgacgggcg cgccgccctt ccccgagtcg tcgctgaccg ccctcctgga tcaccacgtg
     3061 agagattcga tcccatcggt ccaggaggtg cgccccgatc ttccggccgc gctcgatcgc
     3121 gtgatcgggc gcgcgaccgc caaggacgcg gaggatcgat tctccgacgc cctcgagctt
     3181 gcatcggcgg tccgcgcggc gctccagggg ggcgcaccga ccgtcaccgt gctggccggc
     3241 gagatccgca acccctacaa ggggctgcgg gcgttcctcg aggccgacgc ggccgacttc
     3301 ttcggacgcg aaggggtcac gaaacgattg ctccgacggc tcgaggagga tcgacccggc
     3361 tcgcggttcc tcgccgtcgt cgggccgtcg ggttcgggga agtcatcggt ggtccgagcc
     3421 ggtctcgtgc cggcgctccg tcgcgggtcg ctgcccggct ccgagcgctg gtacgtgatc
     3481 gacatgctcc ccggcgctca tccgctccgt gagctcgaat cggcgctgct ggggctggcc
     3541 gtcgcgccgc ctccgtcttt gctggacgag ctcgacggcg acacgcgagg tctcctccga
     3601 gcggtcgacc gcatccttcc cgacccggat gcggaactcc tgatcgtcct cgaccagctc
     3661 gaggaggtgt tcaccctgat cgaggacgag gacgagcggt tccacatcct ggagagcctg
     3721 cgagcggcga ccacggaggc cggcagcagg gtcagggtcg tcgcgacgct ccgcgcggac
     3781 ttcttcgacc agccgctctc ggtccgtggt ttcggcgacc tcctcgccca acgcaccgag
     3841 gcggtcacac cgatgtctcc cgaggagctc gagcgggcga tcgtcgcccc ggcggagcgg
     3901 gcggggctcg cggtcgagcc gcgactgctc gccgcgatga tcgcggacgt cgtggaccgg
     3961 ccgggcgcgc tcccgttgct ccagttcacg ctgaccgagc tcgccgaacg ccgggaggac
     4021 ggcgtgctca cgctcgtcgg ttaccgacgg ctcggcggcg tgtccggcgc cctggcacgc
     4081 cgtgccgagc aaatgttcga ggggatgaac gagggtgccc gggacgcgtg ccggcaggtg
     4141 ttcctccggc tcgtcacgct gggggagggg agcgaggaca cgcgacgccg cgtccgccga
     4201 tcggagctgc agccgctcac ggacgcccgg acgttggacg gcgtgctcga cgcgttcggc
     4261 cgccatcgct tgctctcgtt cgatcgtgat cccgcgaccc gcgagccaac cgtcgagatc
     4321 gcccacgagg cgctgctggg ggtgtgggat cggttgcacg gatggatcga cgaagcccga
     4381 gacgatgtcc gcacgcagcg ccggctcgcg gcggccgtct ccgaatggga atcggccgaa
     4441 cgcgacccga gttacctgct ccgcggggtt cagctcgacc aggctcacga atgggcccga
     4501 accacgacgc tcgtgctctc ggatgcggac agggcctacc tcgccgaggg cgtggaggtg
     4561 cagcgggccg tatccgagcg cgagcgtgcg ctggagcgtc gatcgatgcg gaggctgcgt
     4621 tcgctcatgg cgctgggcgt cgccgcggcg ctcgtcgcga ccacactcac ggtggtcgcg
     4681 gtgagccaac gtagacgagc ggagggcgag gcacgggtgg ctcgggcgcg cgagctcgct
     4741 gccgcggcca ccgccaacct gagcgtcgac cccgagcgca gcatcctgct ggccttggcg
     4801 gccgtcggcg ccacgtggga cgtcgatcgt acggtcgttc ctcaagccga ggaggctctt
     4861 caccgggccc tacaggagtc ccgagtcgtc ctcaccgtgc cgcaagggcg cgagatcgcc
     4921 gtgagcgccg atggcacccg gttcgccacg acaggccagg acgggaccgc gatcgtgtgg
     4981 gaaatggaca ccggcaagcg cctccttacc ctccgcgggc acgagggtga ggtcaacggc
     5041 gtcgccttca gccccgacgg gtctctgttg gccaccaccg gcgacgaccg gacggtgcgc
     5101 ttgtgggatg cggccagcgg ccggcaggtc cacgtgctgc gagggcatcg ggatctggtc
     5161 ttgggcgtcg cgttcagccc cgacgggtcg ctgctagcca cctccagcca ggacggcacc
     5221 gttcggatct gggacgtcgc ggcgggtacg cagcacctcg tgctgagggg tccccccggc
     5281 gagaagttca acaacttttc aggcctcaca cctgccttta gccccgacgg ttccagggtg
     5341 gcgagcgggg ggtggttcag cacgcccatc tgggacctcg cgacggggaa gatctcgatg
     5401 ctccttgccc gccagccagt tgaggccttt gcggtcgcgt tcagccccga cggcacacgc
     5461 gtcgccaccg ccgtccaccg tgacgcgcag acgtgggacg cggagaccgg ggagcccctc
     5521 accgcgttct ccggccacac gggggacatc ctcgcggtcg cctacagccc cgacggcacg
     5581 cggatcgcta ccggcgcgga cgacggg
//
LOCUS       DATBRK010000402         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1530006, whole genome shotgun sequence.
ACCESSION   DATBRK010000402 DATBRK010000000
VERSION     DATBRK010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530006"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VIH70_11915"
     CDS             complement(<1..701)
                     /locus_tag="VIH70_11915"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8117113.1"
                     /translation="MTTQRQPKQLGQILLEQGLISEEHLQRALDEHRNTPKSLGRVLI
                     DLGYIRERDLVRALAEQVGLEFVDLTEYRIDAAAAALLPEALCRRYRALPIGEEDGKL
                     LVAMSDPANVYALDDIRTITGRDVRPVVATANDVEQSIQKFSGMGEQVEALATEAADA
                     IDIDGGVAVEAAVEEAPIVKLVQAIMTQAAADRASDVHIEPTETDVRVRFRVDGVLHE
                     VMHSPKSIQGGLISRL"
     gene            complement(735..1604)
                     /locus_tag="VIH70_11920"
     CDS             complement(735..1604)
                     /locus_tag="VIH70_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117114.1"
                     /translation="MEDFNALAILLAQGMADVRGCLILSRDGLVLGAYPAESERTTTR
                     AWIRFATVGDPERGFVQFGTETWCYVRRAPYAGFAVAGPEERAGLVIDHMEQVLLAAE
                     ESRSRQEGLRASEAATVAPRSVPPSYPELEHPPRDPLVIDAPAPVVERASSGAPPDPP
                     PPFSGPDPEPIDEPVLDHAETSDRSADPFAELGGGANEPDADPPRDPKPPVPLPEPQR
                     WPRLDPRPDAEPELEPVDEADEGPGAGPETGPGAGFEPEDHAEEEDDDVDRFSLAREF
                     GRLLQGGEDPTDG"
     gene            complement(1696..2586)
                     /locus_tag="VIH70_11925"
     CDS             complement(1696..2586)
                     /locus_tag="VIH70_11925"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY8117115.1"
                     /translation="MSDVPDRPGRVRGSTRSVGVLGWPVEHSLSPAIHNAAFVALGLD
                     WVYVPLPVAPAELPAALRGLGALGFAGANVTMPHKTRTAELIEDLSEDARLLRAANTI
                     VVSADGLAGHNSDAVGFERFLRDDAGFDASGRSALLFGAGGGARACALAAARSGLASL
                     SVAVREGSRADDLRAALEGSATEVRVVGFDDASRFEVDLIVNATPLGVHGEALPLPPL
                     QPGVVGVDLLYRPGVTPFRRAVRDAGGAAFGGLGLLLQQAAISFELWTGQSAPLPVMS
                     AAALGELAEPGIAAPAATRD"
     gene            complement(2579..>3626)
                     /gene="mltG"
                     /locus_tag="VIH70_11930"
     CDS             complement(2579..>3626)
                     /gene="mltG"
                     /locus_tag="VIH70_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY8117116.1"
                     /translation="DDDARPRHRSERRANWGFRIFLIVFLVLAGLAIAGNRYYGWCSE
                     ASGPRDPVAFRVRKGASGSEVVGDLHDRGVLRCGLVSTWLLRRSGLEDSLRAGSFELT
                     TNMTPDEAFEVLTTPPEPVPTARLTIPEGYRLTQIADRVEHDLGIPAKRFLKATEQGD
                     WSLPPYLPEDATSIEGFLFPETYFVRKDADADMVIRRLLDQFGTEAESLPWANAEQLG
                     VTPYEVVIIASMIEREAALDRERPIIAGVIYNRLRLGMTLGIDATLLYDDPTPDGQLS
                     YSDLEYDSPYNTRINGGLPPTPIASPGRASLEAALQPANTPYLFYVLCGEDGHHAFGK
                     TNAEHEANRVRCDE"
BASE COUNT          502 a         1314 c         1296 g          514 t
ORIGIN      
        1 aggcggctga tcagcccgcc ctggatgctc ttgggcgagt gcatgacctc gtgcaggacg
       61 ccgtccacgc ggaaccgcac acggacgtcg gtctccgtcg gctcgatgtg cacgtccgac
      121 gctcggtcgg ccgcggcctg cgtcatgatc gcctgcacga gcttgacgat cggggcttcc
      181 tcgaccgccg cctcgaccgc gacgccgccg tcgatgtcga tcgcatcggc ggcctccgtc
      241 gcgagcgcct cgacctgctc tcccatcccc gagaacttct ggatcgactg ctcgacgtcg
      301 ttcgccgtgg cgaccaccgg acgaacgtcc cggccggtga tcgtccggat gtcgtcgagc
      361 gcgtacacgt tcgccgggtc cgacatggcc accaggagct tgccgtcctc ttccccgatc
      421 gggagggcgc ggtagcggcg gcacagcgcc tccggcagca gggccgccgc cgcggcgtcg
      481 atccggtact cggtcaggtc gacgaactcg aggcccacct gctcggcgag cgctcgaacg
      541 aggtcccgtt cgcggatgta gccgaggtcg atcagcaccc ggccgagcga cttgggcgtg
      601 ttgcgatgct catcgagggc gcgctggaga tgctcctcgg agatgaggcc ctgctcgagc
      661 aggatctgcc cgagctgctt cggttgtcgc tgggtggtca ccatgtggag ctaggccctt
      721 tggtccctgg tcgcctaacc atcggtaggg tcttcgcccc cctgaagcag ccggccgaac
      781 tcccgcgcga gggagaatcg gtcgacgtcg tcgtcctcct cttcggcgtg gtcctcgggc
      841 tcgaacccgg ccccggggcc cgtctccggg cccgctcccg gcccctcgtc cgcctcgtcg
      901 acgggttcga gctccggctc cgcgtcgggt cgcgggtcga ggcggggcca gcgctgcggt
      961 tcggggagcg ggacgggcgg cttcgggtcc cggggcgggt cggcatccgg ctcgttcgcg
     1021 ccgcctccga gctcggcgaa ggggtcggcc gaccgatcgg aggtctccgc gtggtcgagg
     1081 acgggttcgt cgatcggctc cgggtccggg ccggagaacg gcgggggcgg atcgggcggg
     1141 gcgccgctcg aggcgcgctc gacgaccggt gcgggggcgt cgatcaccag gggatcccgc
     1201 ggggggtgct cgagctccgg atacgatggc ggcacggatc gaggggccac cgtcgcggcc
     1261 tcgctcgcac gcagcccctc ctgccgcgac cggctctcct cggccgccag cagcacctgc
     1321 tccatgtggt cgatcaccag gccggcgcgc tcttcgggcc cggcgacggc gaacccggcg
     1381 tacggggccc ggcggacgta gcaccacgtc tccgtgccga actggacgaa gccgcgttcg
     1441 gggtcgccca ccgtggcgaa ccggatccag gcgcgcgtag tggtgcgctc cgactcggcc
     1501 ggatacgccc cgaggaccaa cccgtcgcgg gacaggatca ggcacccgcg aacgtcggcc
     1561 atgccctgcg cgagcaagat cgcgagcgcg ttgaagtctt ccatatggac ggcgacctcc
     1621 tttcggaacc atcgggaggc acgagtgggc gcttgaggga cacccccgtc cggcggaggc
     1681 cgaacggtcc ggttctcagt ctctagtcgc cgccggcgcc gcgataccag gctccgcgag
     1741 ctcgccgagg gcggccgccg acatcaccgg aagcggcgcc gactgacccg tccagagctc
     1801 gaacgagatc gccgcctgct ggagcaacag cccgagaccc ccgaacgccg cgccgcccgc
     1861 gtcgcgcacg gcacgccgga acggggtcac gcccggccgg tagagcaggt cgaccccgac
     1921 gaccccgggc tgcagcggcg ggagcgggag cgcctcaccg tggacgccga gcggcgtcgc
     1981 gttcacgatc aggtcgacct cgaaccggga ggcgtcgtcg aacccgacca cccgcacctc
     2041 ggtggccgag ccctcgagcg cggcgcgcag gtcgtcggcc cgcgagccct cgcgcacggc
     2101 cacggacagg ctcgcgagcc cgcttcgagc ggcggcgagc gcacacgcgc gcgcgcctcc
     2161 tcccgccccg aacagcagcg cggaccggcc ggacgcgtcg aaccccgcgt cgtcgcggag
     2221 gaaccgctcg aacccgaccg cgtccgagtt gtgacccgcg agcccgtccg cgctcacgac
     2281 gatcgtgttg gccgcccgca gcaggcgcgc atcctcggac aagtcctcga tcagctcggc
     2341 ggtccgcgtc ttgtgcggca tcgtcacgtt cgcgccggcg aacccgagcg cgccgagccc
     2401 gcggagcgcg gccggaagct ccgcgggcgc gaccgggagc gggacgtaga cccagtccaa
     2461 gccgagcgcg acgaaggcgg cgttgtggat cgccggcgac aggctgtgct cgacgggcca
     2521 cccgagcacg ccgaccgatc gcgtcgaacc ccggacgcgc cccggccggt cggggacgtc
     2581 actcatcgca gcggacccgg ttggcctcgt gctcggcgtt ggtcttcccg aacgcgtggt
     2641 gcccgtcctc gccgcacagc acgtagaaga ggtagggcgt gttcgccggc tgcagggccg
     2701 cctcgagcga ggcccggccg gggctggcga tcggcgtcgg cggcagccca ccgttgatcc
     2761 gcgtgttgta ggggctgtcg tactccaggt cggagtagct gagctggcca tccggcgtcg
     2821 ggtcgtcgta caggagcgtc gcgtcgatgc cgagcgtcat ccccagcctc aatcggttgt
     2881 agatcacgcc agcgatgatc gggcgctcgc gatccagcgc cgcctcccgc tcgatcatcg
     2941 acgcgatgat caccacctcg tacggcgtca ccccgagctg ctccgcgttg gcccacggca
     3001 atgactccgc ctcggtgccg aactggtcga gcagcctccg gatgaccatg tcggcgtcgg
     3061 cgtccttgcg aacgaaatag gtctcgggga acaggaagcc ctcgatgctc gtggcgtcct
     3121 ccgggaggta gggaggcaag gaccaatccc cctgttccgt cgccttcaga aaccgcttgg
     3181 cggggatccc caggtcgtgc tcgacccgat cggcgatctg cgtcaggcgg tatccctcgg
     3241 ggatcgtgag gcgggccgtc gggacgggtt cgggcggggt cgtgaggacc tcgaacgcct
     3301 cgtcgggcgt catgttcgtg gtcagctcga agctccccgc gcgaagcgag tcctctaggc
     3361 cgctcctgcg gaggagccac gtcgagacca ggccacaccg gaggaccccc cggtcgtgga
     3421 ggtcgccgac gacctccgac ccgctcgccc cctttcggac ccggaacgcg accggatccc
     3481 tcgggcccga cgcctccgag caccagccgt agtagcggtt gccggcgatg gccaggccgg
     3541 cgagcaccag gaagacgatc aggaagatgc ggaagcccca gttggcccgc cgttcggatc
     3601 ggtggcgcgg gcgagcgtcg tcgtcg
//
LOCUS       DATBRK010000403         7447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_234139, whole genome shotgun sequence.
ACCESSION   DATBRK010000403 DATBRK010000000
VERSION     DATBRK010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234139"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1814)
                     /locus_tag="VIH70_11935"
     CDS             complement(<1..1814)
                     /locus_tag="VIH70_11935"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8117117.1"
                     /translation="MRPRTASRLAWSVGIFSIALMIGALVLMFGYRDATLPATVSEFR
                     WNFPNVFNEVVNLGLPVIGVVLASRRPENRIGWLFLAAALLIGLYSFGLSYAVRAVFV
                     APGSLPLGQGIAWVGTWMLVIAIGLLPFLFLLFPTGHLPSPRWRPAGWLMGGSFALST
                     LGTLALWTQSRNEPFGPSTPGHRTLLLLLALPVAGSLLLSIVAVVVRFLGSSGDERLQ
                     LKWLATAAVLLVGTFILPLPTYACLLCSPPAVVYVTQNLAVVFGWVSIAIAVLKYRLY
                     EIDVVINKAVVYGTLAVFITLVYVGLVAGVGTLVGNTRNPLLAAIAAAVIAVAFQPIR
                     QRAGRLANRVVYGKRATPYEVLSDFAEQMTGAYPIEDVLPHIARMLTEGTGAIRSDVW
                     LLVGAELRVAGSWPLAEVAPVPVIANGSIDVPGAVAVRVRYQGEVLGALSLQKPPGDP
                     ITSTELKLLDDVASQAGLVLRNARLIGELRASRQRIVSAQDAAARRLERNIHDGAQQQ
                     LVALAVKTRLADSLVGRDEAKAHDVLSQIQTEAQEALENLRDLARGIYPPILADQGLA
                     VALDAQAKRSPVPVAVEAVGISRYRSETEAAVYFSVLEA"
     gene            complement(1955..2263)
                     /locus_tag="VIH70_11940"
     CDS             complement(1955..2263)
                     /locus_tag="VIH70_11940"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8117118.1"
                     /translation="MPIFVYLRFCLAETADRQDFEGDLRAMAELAETQPGYLWSDMGP
                     SMVEPSVYVVVSEWYGIDHVRAWEHEATHEEIQHKWEAHFREPLVHRRFTSWERPPAT
                     "
     gene            complement(2392..3561)
                     /locus_tag="VIH70_11945"
     CDS             complement(2392..3561)
                     /locus_tag="VIH70_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117119.1"
                     /translation="MAETDPTPTGGTSVVDAKTVAAERRAARAESRPRWLRVALKLAT
                     FAAALFLFVLAIQLMKEGAKALAPTLEDSPLFSNAVSTLGFGWLGAYIVLSGSPIAAV
                     ALSLFVGGATTELQTFTMLSGSRLGASFVVLLVGFLYALRNRGRNRGESIGMGVLALS
                     LTAIVYVPGMLIGYAILKSGVLGGIHWSASGDVLSVVDVIWGPAVDVITRLPGWTLLP
                     IGLGLILLAFRLLDNVLPQLDGERHGGDKGSWMKRPWPMFGLGCLVATLVLSVSVALT
                     LLVPLASRGYITRRESIPYIMGANITTLADTLVAAMILGNPVAVQIVLAEAIAVSIVS
                     LFYLAVLYGPLTRGIMALDEWVVSTNRRLWLFVAILFVLPALFLTSGIWIGPIAH"
     gene            complement(3667..4518)
                     /locus_tag="VIH70_11950"
     CDS             complement(3667..4518)
                     /locus_tag="VIH70_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8117120.1"
                     /translation="MAADQMTADRPFSLVLAGVDGSERAVEAARQAARLASAAGAHLI
                     VAYVIEDRPYEEGDAERALEAASAVARTAGADPEARTLTGDDAGDALVEEAERDGVDL
                     LCVGPDSELLGGAIRFGHVAGKILRDATCSVLIARPAGPAFPRQIACAIDGSDASVLT
                     ALAAAGVARATGAELRLVHVVPVFRGRNEEWTLGPQDQSPPELEPSVKAVSSLGLEPV
                     REMAMGRPERTLVHVAERDEVDLVVVGHRGVSGMTRRLLGSVSEHVGHHAPCSVFIVR
                     PSVAGRA"
     gene            4612..5058
                     /locus_tag="VIH70_11955"
     CDS             4612..5058
                     /locus_tag="VIH70_11955"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY8117121.1"
                     /translation="MFRGDDTPEVSVVGPGTRIEGTVVAAGSLRVEGEVKGKITAERE
                     VTLSPQGRVEANIQAGSIVLAGQVKGNLAAKGDVSLPADSRLDGNIRGHNVDVGGVVM
                     GDIVGKGRVALGPRARVEGQITSTSLAIAEGAVFIGSSTMGEETRT"
     gene            complement(5055..5303)
                     /locus_tag="VIH70_11960"
     CDS             complement(5055..5303)
                     /locus_tag="VIH70_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY8117122.1"
                     /translation="MTARPLTCREVIILLTDYVEDALPEQERRRVEAHLASCDGCTTY
                     LEQMRETIRLTGMLTEEQIPDAERERLLDAFRGWTRPR"
     gene            complement(5300..5959)
                     /locus_tag="VIH70_11965"
     CDS             complement(5300..5959)
                     /locus_tag="VIH70_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8117123.1"
                     /translation="MSAPGVVASKEDLAIVERLRAGDELAFMMLVEQHTAAMLRIARM
                     YVSSRAVAEDVVAEAWLGVVRGIERFEGRSSLRTWIYRIVANIAKTRGAAEGRSVPFS
                     SLAGDDQTDRGVEPSWFFDANGRNPGGWTTFPEDWRGIPEARLIGNETLRHIGRAIDH
                     LPPLQAEVIRLRDVLGWSADEVRDALDLSEVNQRVLLHRARTRVRRELDEYLRTGSED
                     A"
     gene            complement(5974..7026)
                     /locus_tag="VIH70_11970"
     CDS             complement(5974..7026)
                     /locus_tag="VIH70_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY8117124.1"
                     /translation="MIVIGIDPHKQTHTAVAIDRATGEVLEELTVRARRPGFERLVAW
                     ARNLHDERLFAIEDGRHVSGHLERYLIGRGEHSVRVPPRLMGEARRVDRVRGKSDPID
                     ATAVARAALAHPDLPTATLAGPEREIGLLAAHREQMVNERTAIQSKLRWLLHDVDPDL
                     APGPRTLDRFCTLDRLGARLEQMAPTADVRICLELLDRCRQITVRTNELEREIASLVE
                     AAAPSLLQIPGCAALTAAKVVAETARVERFPTEGHFARYAGTAPVPASSGATHRHRFN
                     RRGNRQLNAALHRIAVTQLRIHQPAKDYVAKKRSEGKSKTEAIRALKRRLSRVVFKTM
                     TSTSRPTDDLIPVVAA"
BASE COUNT         1248 a         2600 c         2562 g         1037 t
ORIGIN      
        1 gcctcgagga cgctgaagta cacggctgcc tccgtctccg agcggtaccg ggagatcccg
       61 accgcttcga cggccacggg aaccggggat cgcttggctt gcgcgtccag ggcgacggcg
      121 agtccctggt cggcaaggat cggtggatag atgcctcggg caaggtcgcg aaggttctcg
      181 agggcctctt gggcctcggt ctggatctgc gagagcacgt cgtgcgcctt cgcctcgtcg
      241 cggccaacca gggagtcggc cagacgcgtc ttcaccgcca gggccacaag ttgctgctgt
      301 gcgccgtcat ggatgttgcg ttcaaggcga cgcgccgccg cgtcctgggc cgacacgatc
      361 cgctgacggg aagcccgaag ctccccgatc aggcgtgcgt ttcgaaggac cagccccgcc
      421 tgtgatgcga cgtcgtcgag gagcttcagc tcggtcgagg tgatcggatc gcccggcggc
      481 ttctgcaggg agagcgctcc cagcacctct ccctgatacc ggacccgcac ggccaccgct
      541 cccggaacgt cgatcgaacc gttcgcaatg acgggaacgg gcgcgacctc tgcgagcggc
      601 caggagcccg ccacccgaag ctccgcaccc acgaggagcc acacatcgga ccggatcgcg
      661 cccgtgccct cggtcagcat ccgagcgata tggggaagga cgtcttcgat gggataggcg
      721 ccggtcatct gctctgcgaa gtcggacaac acctcgtacg gagtcgcccg cttgccgtag
      781 acgacgcggt ttgccaggcg tccggcgcgc tgccggatgg gctggaaggc gacggcgatc
      841 acggccgcgg cgatcgcggc cagcaagggg ttccgcgtgt tgccgacgag cgtccccacg
      901 ccggccacca ggccgacgta cacgagggtg atgaaaacgg ccaacgttcc gtagaccacc
      961 gccttgttga tgacgacgtc gatctcgtag aggcggtact tgagcacggc gatcgcgatc
     1021 gacacccagc cgaacacgac ggccaggttc tgcgtcacgt agacgaccgc gggagggctg
     1081 cagaggaggc aggcgtacgt ggggagcgga aggatgaacg tgccgacgag gagcaccgcc
     1141 gcggtcgcca gccacttgag ctggaggcgc tcgtcccccg atgaccccag gaagcgaacg
     1201 acgaccgcga cgatcgagag caggagcgaa ccggcgaccg gaagcgccag cagaagcagc
     1261 agcgttcgat ggcccggtgt cgatgggccg aagggctcat tccgggactg ggtccacaag
     1321 gcgagggtgc ccagagtgga cagcgcgaac gagccgccca tcaaccagcc tgccggacgc
     1381 cagcgcggcg aggggagatg acccgtgggg aacaagagga agaggaaggg aagcaggccg
     1441 atcgcgatga ccagcatcca ggtcccgacc cacgcgatcc cttgtcccag gggtagcgat
     1501 ccgggggcga cgaagacggc tcggaccgcg taggagaggc cgaacgagta gagcccaatc
     1561 aacaacgcag cggccaggaa caaccaccca atccggttct ccggtcgcct ggacgcgagg
     1621 acgaccccga tcacggggag gccgaggttg accacttcgt tgaagacgtt cgggaagttc
     1681 caacgaaatt cggagaccgt ggctggcagc gtcgcgtcgc ggtagccgaa catgaggacg
     1741 agcgcgccga tcatcagcgc gatcgagaag atcccgaccg accaagcgag cctggacgcg
     1801 gtcctaggtc tcatcctcga ggtcgattgt gccgccgatg cgctcggaaa cggaagggtg
     1861 gaggcgcgac cgaccatgca ggggcaagcc actcgcccgg tcgagtggtg ccacgaggat
     1921 tcaccctcgg acgccgacgg ccctttcctc cgtcctacgt cgccggcggt cgctcccaag
     1981 acgtgaagcg gcggtgaacg aggggctcgc ggaagtgcgc ctcccactta tgctggatct
     2041 cttcatgcgt ggcctcgtgc tcccaggcac ggacgtgatc gatcccgtac cactcggaca
     2101 cgaccacgta caccgacggt tcgaccatcg agggccccat atcggaccag aggtacccgg
     2161 gttgcgtctc agccagctcg gccatggccc tcaggtcgcc ctcgaagtcc tgtcgatcgg
     2221 ccgtctcggc aagacagaag cgcagataca cgaagatcgg cacggggcag cctcctcagc
     2281 ttcctacctc aagcctcgaa ttctcgggaa cccggagcct catgatcggc ctgctgacaa
     2341 agcgagcggt cgggcagacc gagaccggtt cgagcccccg agcggcaacc gtcagtgggc
     2401 gatcgggccg atccagatgc cggacgtgag gaacaacgcc ggcaacacga acaggatcgc
     2461 gacgaagagc cacagccgcc ggttcgtcga caccacccac tcgtccagcg ccatgatccc
     2521 gcgcgtgagc ggtccgtaca gcaccgcgag gtagaacagc gacacgatgc tcacggcgat
     2581 cgcctcggcg aggacgatct gcacggcgac cgggttcccg aggatcatcg cggccaccag
     2641 cgtgtcggcg agcgtcgtga tgttcgcgcc catgatgtag gggatcgact cgcgccgcgt
     2701 gatgtagccg cggctcgcga gcggcaccag cagcgtgagc gccaccgaga ccgagagcac
     2761 cagcgtcgcg accaggcacc cgaggccgaa catcggccag ggccgcttca tccacgaccc
     2821 cttgtctccg ccgtgccgct cgccgtccag ctgcgggagc acgttgtcca gcaaccggaa
     2881 cgccagcagg atcagcccca gcccgatcgg cagcagcgtc cagcccggca gcctcgtgat
     2941 gacgtcgacc gcggggcccc agatcacgtc gacgaccgac agcacgtcgc cggaggcgct
     3001 ccagtggatc cctccgagca ccccggactt caggatggca tagccgatca gcatgccggg
     3061 cacgtacacg atggcagtga gcgagagcgc gagcaccccc atcccgatcg actcgccgcg
     3121 gttgcggccg cggttgcgca gcgcgtacag gaacccgacc agcaggacca cgaacgacgc
     3181 gcccagccgc gaacccgaga gcatcgtgaa cgtctgcagc tccgtggtcg cgccgcccac
     3241 gaacaggctg agcgcgaccg ccgcgatcgg ggagcctgac agcacgatgt acgcgcccag
     3301 ccacccgaac ccgagcgtgg agaccgcgtt gctgaagagc gggctgtcct cgagtgtggg
     3361 cgcgagcgcc ttcgcgccct ccttcatcag ctggatcgcc aacacgaaca ggaacagcgc
     3421 ggccgcgaac gtcgccagct tgagcgcgac gcgaagccac ctgggccggc tctcggcgcg
     3481 tgccgcccgg cgttcggccg cgacggtctt cgcgtcgacg acggatgtcc cgccggtcgg
     3541 tgtggggtcg gtttcggcca tggctggttc ggtatggccc gtcccgggcg ggcggtctgg
     3601 tcggcgggaa gggcgcaacc ctactcgccg cctcgtggag gccacaaggg tcgaaaggtc
     3661 cgatgttcac gcacggcccg caaccgaggg ccgcacgatg aacaccgagc agggagcgtg
     3721 gtggcccacg tgctcgctca cgctgccgag cagccggcgc gtcatcccgc tcacgccccg
     3781 atgacccacg accacgaggt cgacctcgtc gcgctccgcc acgtgcacca gggtccgctc
     3841 gggtcgaccc atcgccatct cgcggaccgg ctcgaggccg aggctcgaca cggccttcac
     3901 ggagggttcg agctccggcg ggctctgatc ctgcggtccg agcgtccact cctcgttgcg
     3961 cccccggaag accggcacga cgtgcaccag tcggagctca gcgccggtgg cccgggcgac
     4021 gccggcggcg gcgagcgccg tgagtaccga tgcatccgac ccgtcgatcg cgcaggcgat
     4081 ctgccggggg aacgccggtc ccgccggccg cgcgatcagg accgagcagg tggcgtcgcg
     4141 caggatcttt ccggcaacat ggccgaaccg gatcgcgccc ccgagcagct ccgagtcggg
     4201 cccgacgcac agcaggtcga cgccgtcgcg ttcggcctcc tcgaccaggg cgtcgccggc
     4261 atcatcgccg gtcagggtcc gcgcctccgg gtcggcgccg gcggtccgcg cgaccgcgct
     4321 ggcggcctcg agggcccgct cggcgtcccc ctcctcgtac gggcgatcct cgatgacgta
     4381 ggcgacgatc aggtgcgcgc cggcggccga ggcgaggcga gcggcctggc gcgcggcttc
     4441 cacggcgcgt tccgacccgt cgacgcccgc gaggacgagc gagaacggcc ggtcggcggt
     4501 catctggtcg gcggccacga cggcattgtc tcccagggcc ggcccgccgg gccaaccgtg
     4561 ccgcgacggg gtctcgcgag gacggatacg ctgcggtcca ggaggtgctg catgttccgt
     4621 ggagacgaca cccccgaggt ctcggtggtc ggtccgggca cccggatcga gggcaccgtc
     4681 gtggcggccg gctccctcag ggtcgaggga gaggtcaagg gcaagatcac cgccgaacgg
     4741 gaggtgacgc tctcccccca gggccgggtg gaggcgaaca tccaggcggg gagcatcgtc
     4801 ctggccgggc aggtcaaggg caacctcgcc gccaagggcg acgtatccct tccggccgac
     4861 agccggctcg acgggaacat ccgaggccac aacgtcgacg tgggcggtgt ggtcatgggc
     4921 gacatcgtcg gcaagggcag ggtggcgctc ggcccacgcg ctcgcgtcga gggtcagatc
     4981 acctcgacgt cactcgcgat cgccgaggga gccgtcttca tcgggagctc caccatgggc
     5041 gaggagacgc gcacctagcg gggccgggtc cagcctcgga acgcgtcgag gagccgctcc
     5101 ctctccgcgt cggggatctg ttcctcggtc agcatcccgg tcagccggat cgtctcccgc
     5161 atctgctcga ggtaggtcgt gcaaccgtcg cagctcgcga ggtgcgcctc gacccgccgt
     5221 cgctcctgct cgggaagtgc gtcctcgacg tagtcggtaa gcaggatgat cacctcgcga
     5281 caggtcaggg gtcgcgcggt cacgcgtcct ccgatccggt cctcaggtac tcgtccagct
     5341 ctcggcgcac acgcgtgcgc gcgcgatgca ggaggacccg ctggttgacc tcggagaggt
     5401 ccagggcgtc gcgcacctcg tcggcgctcc acccgaggac gtcacgcagc cggatcacct
     5461 ccgcctgcaa cggcggcagg tggtcgatcg cacgcccgat gtgtcgcagg gtctcgttgc
     5521 cgatgagccg ggcctcgggg atcccgcgcc agtcctccgg gaaggtcgtc catccgccag
     5581 ggttgcggcc gttcgcgtcg aagaaccacg agggctcgac cccccggtcc gtctggtcgt
     5641 cgcccgcgag cgaggagaac gggacggagc ggccctccgc cgctcccctg gtcttggcga
     5701 tgttcgcgac gatccggtag atccacgttc gcagcgagga gcggccctcg aagcgctcga
     5761 tgcccctgac cacgccgagc catgcctccg cgacgacgtc ctcggcgacg gcgcgcgacg
     5821 acacgtacat ccgcgcgatg cgcagcatgg ccgccgtgtg ctgctcgacg agcatcatga
     5881 acgcgagctc gtcgccggct cgaaggcgct cgacgatcgc caggtcctcc ttggaggcga
     5941 cgacgccggg ggcgctcacg agttgctcct atgtcaagcc gcgacgactg gaatcaagtc
     6001 gtcggtcggc cggctcgtcg atgtcatcgt cttgaacacc accctggaga gtcgtcgctt
     6061 gagggctcgg atggcctcgg tcttgctctt gccctccgag cgcttcttgg caacgtagtc
     6121 cttggccggt tggtggatcc ggagctgggt gacggcgatc cgatgcaggg ccgcgttcag
     6181 ctgccggttg cctcgccggt tgaaccgatg ccggtgcgtg gcacccgagg aggccgggac
     6241 cggtgccgtg cccgcgtaac gagcgaaatg cccctcggtc gggaaccgct cgacccgggc
     6301 ggtctcggcc acgaccttgg ccgccgtcag cgccgcgcac cctgggatct gaagcagcga
     6361 gggcgcggct gcctcgacga gcgatgcgat ctctcgctcg agctcgttcg tccgcacggt
     6421 gatctgtcgg catcggtcga gcagctccaa gcagatccgc acgtcggcgg tgggcgccat
     6481 ctgctcgagc cgtgccccga ggcgatcgag cgtgcagaac cggtccagcg tgcgagggcc
     6541 cggagccaga tcggggtcga catcgtgcag gagccaccga agcttggact gaatggcggt
     6601 gcgctcatta accatctgct cccggtgcgc cgcaagcagt ccgatctccc gctccggacc
     6661 cgctagggtc gccgtgggca ggtcggggtg cgcaagcgcc gcccgcgcca ccgcggtcgc
     6721 atcgatcgga tcggacttgc cgcggacccg atcgactctg cgagcctcac ccatgagccg
     6781 aggaggcacc cgcaccgagt gctccccgcg accgatcagg taccgctcca ggtgcccgga
     6841 gacatgccgg ccgtcctcga tcgcgaacag gcgctcgtcg tgaaggttcc gtgcccacgc
     6901 cacgagccgc tcgaagccgg ggcggcgggc gcgcaccgtg agttcctcga gcacctcgcc
     6961 ggtcgctcgg tcgatcgcga cagccgtgtg ggtctgctta tgcgggtcga tcccgattac
     7021 gatcacctcc ggtccccttt ctggacgcca catcccgatg gcagggacca ccggcggaca
     7081 cgcctcagtg gggcggatgc acgctcctat gaagtcacgc cggcaggcct cggccggcgg
     7141 cgggcggcag aacccgtgaa ggtcatcccg ctcgcggggt gacacgaagg ctatgagcca
     7201 gcccgccgtc ggccgaaggc tatcccgagc cgaacgcggg atctcactgc ccgctcaccc
     7261 tgacactgag ttggaggcta cggcgtcgaa ccgtcgcccg cgttcgacca cgccagcctc
     7321 atcaaaacgc ccctcggccg tagcggcgag ttgcacgcga agcgccatcg ccatcacgcc
     7381 ggcatctcgc agcgcagccg cccactcctg cgccgcagcc tagcccgccg agacgcggtg
     7441 agcgagg
//
LOCUS       DATBRK010000404         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1965592, whole genome shotgun sequence.
ACCESSION   DATBRK010000404 DATBRK010000000
VERSION     DATBRK010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965592"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VIH70_11975"
     CDS             complement(<1..860)
                     /locus_tag="VIH70_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY8117125.1"
                     /translation="MIEPGRQDVARPMAGDPDGERPVHLSTAAGRGGIVDEFNGWLSL
                     LVERDGSDLHVKVGTPPKMRLSGALLPLDREPLSHDEMEAIGTAIVPSERVERFEAAG
                     EVDFAHSLPGVGRFRINVFRQRGSVSAVARTLRFGGPSFDEMGLPEVIRRLADEQRGL
                     VLVTGPTGSGKTTTLAAMIEHLNHTRQSHIVTIEDPIEVLFRDDVASINQREVGSDTE
                     SFLSALRAALRQDPDVILIGEMRDTETVRTSLQAAETGHLVLSTLHTVDATETVNRVV
                     DFFPPEQQGQ"
     gene            complement(952..>1660)
                     /locus_tag="VIH70_11980"
     CDS             complement(952..>1660)
                     /locus_tag="VIH70_11980"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8117126.1"
                     /translation="ADRQEPPVPAREPTPLEIHKALADDTRFRLYRYLGLSGRPVSVR
                     ELSTRLSLHPNTLRPHLRRLEEAGLVRREVRKGATVGRPQTMYVAVGQIERENRDFRL
                     LAEILAGMVSGARARTRATERAREWGRYLAARGVPKPGTRAAARHNLAVLQEAMASAG
                     FDPRFRRASDGLVEVTLRDCPFRELLDDHRDLVCSIHRGLVEGMLGALEPPLALEAFT
                     PLSERAICRVEVREPRA"
BASE COUNT          226 a          584 c          612 g          238 t
ORIGIN      
        1 atctgtccct gctgctcggg cgggaagaag tcgacgacgc ggttcacggt ctcggtcgcg
       61 tccacggtgt ggagcgtcga caagaccagg tggccggtct cggccgcctg cagcgacgtc
      121 cgcacggtct cggtgtcccg catctcgccg atcaggatca cgtcggggtc ctgccgcagg
      181 gcggctcgga gcgccgagag gaagctctcg gtgtcgctgc cgacctcgcg ctggttgatc
      241 gacgccacgt cgtcgcggaa caggacctcg atcgggtcct cgatcgtcac gatgtggctc
      301 tgccgggtgt ggttgaggtg ttcgatcatc gcggccaggg tcgtggtctt gcccgagccg
      361 gtcggaccgg tcacgaggac gaggccgcgc tgctcgtccg cgagccgacg gatcacctcg
      421 ggtaggccca tctcgtcgaa gctcggcccg ccgaaccgga gcgtccgggc cacggccgac
      481 acggagccac gctggcggaa cacgttgatg cggaagcgtc cgacgccggg aagcgagtgg
      541 gcgaagtcga cctcgcccgc ggcctcgaac cgctcgaccc gttcggacgg cacgatggcg
      601 gtgccgatcg cctccatctc gtcgtgagac aggggctctc ggtcgagcgg gagcagggcg
      661 cccgacagac gcatcttcgg cggcgtgccc accttgacgt gcaggtcgga gccgtcgcgc
      721 tcgaccagca gcgagagcca gccgttgaac tcgtccacga tgccgccgcg gcctgcggcc
      781 gtggacaggt ggaccggacg ttcgccgtcg gggtcgccgg ccatcggccg ggcgacgtcc
      841 tgccgcccgg gctcgatcac ctgatgaact cctgagcgtg gccgggcatg gtccctgcgt
      901 ctatcggccg ccaccggccg tcccctgagc cctccggctc aagcctccgg ctcaagctcg
      961 gggttccctg acctccaccc ggcagatcgc ccgctcggac aggggcgtga acgcctcgag
     1021 ggccaggggc ggctccagcg ccccgagcat gccctcgacg agcccgcggt ggatcgagca
     1081 gacgaggtcg cgatggtcgt ccagcaactc ccggaacggg cagtctcgaa gcgtgacctc
     1141 gacgagcccg tcgctcgccc tgcggaagcg cgggtcgaac ccggcggagg ccatcgcctc
     1201 ctggagcacc gccaggttgt gccgggcggc ggcgcgagtg ccaggcttcg ggacgccgcg
     1261 cgcggcgagg taccggcccc actctcgtgc gcgttcggtg gcacgggtcc gggcccgcgc
     1321 gcccgagacc atgccggcca ggatctccgc gagaaggcgg aagtcgcggt tttcgcgttc
     1381 gatctgcccg accgcgacgt acatcgtctg cgggcgcccg accgtcgcgc ccttgcggac
     1441 ctcgcgccgg accaggccgg cctcctcgag tcggcgcagg tgcgggcgca gcgtgttcgg
     1501 gtgcagggac aggcgcgtcg agagctcgcg gaccgagacc ggacggcccg acagcccgag
     1561 gtagcgatac agccggaacc gcgtgtcgtc ggcgagcgcc ttgtggatct cgagcggggt
     1621 cggctcacgg gcgggcacgg gcggctcctg gcgatcggcg
//
LOCUS       DATBRK010000405         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_235622, whole genome shotgun sequence.
ACCESSION   DATBRK010000405 DATBRK010000000
VERSION     DATBRK010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235622"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VIH70_11985"
     CDS             complement(<1..618)
                     /locus_tag="VIH70_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8117127.1"
                     /translation="MSVPAERPGGLIRRPHATTGLWSWFTTIDHKKIGVLYGITAFAF
                     FLIGGSEALLIRVQLARPDQSFLEPAVYNELFTMHGITMIFLVVMPLSAAFFNYLLPL
                     MIGARDVAFPRLNAFSYWAFLFAGLFIYSSWFLGGAPNGGWFGYAPNSVIDPTRGMTF
                     YAIGLLIAGIASTVSAINLAVTILNMRAPGMTLFRMPVFVWMGLVV"
     gene            complement(640..1767)
                     /locus_tag="VIH70_11990"
     CDS             complement(640..1767)
                     /locus_tag="VIH70_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY8117128.1"
                     /translation="MRRRTVVVLGLVALALVAAACAPNATQSALEPKGQYAQKSFDLF
                     VPVFWVAVAIFVIVEGLLVLFVFRYRHRKNRTPRIPAQVHGNTRLEIAWTILPTLILA
                     GVAVPTIATIWDLAAEPTGDVLEVNVLAHQWWWEFDYPGQKILTANELHIPTGRPVYL
                     TLCAVGFGYGTPVPSQGGPPAPNACQTGPPDGQPPAGIGAAVIHSFWVPQLAGTQDVV
                     PDLTNHLTIQADEPGTYQGQCKEFCGLSHAYMRFRVVAHTPADFERWVAEQKAPASPD
                     VSGPAVAGKEIFQQTCTACHAVAGLETANGQPALANGAPNLTHLMSRECFRGCTLETN
                     DENLARWIANPRAVEAGSFMPDYGLTPDQVDQVVAFLNTLE"
     gene            complement(1895..2641)
                     /locus_tag="VIH70_11995"
     CDS             complement(1895..2641)
                     /locus_tag="VIH70_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY8117129.1"
                     /translation="MTGVLADWVQDGLGSWWAPGLAFVAGVISFASPCVFPLVPGYLS
                     FVAGGEVREERQPVVPILLFIAGFAAVFSALGAFTGAMTRLLRSTAGIRISGAIIIAF
                     GVLMLLYALRLGSPSLFLERRPLLERARPGRAWAFPLGVAFGAGWTPCIGPVLAGVLA
                     IAGSQGGSLRGALLLFVYSMGLGLPFLLVGVGIRKLMGALAFVKRNYQWIAGVSGVVM
                     IAIGVLVATNLWTRMLSPVFRAIDDFSPPI"
     gene            2889..3239
                     /locus_tag="VIH70_12000"
     CDS             2889..3239
                     /locus_tag="VIH70_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117130.1"
                     /translation="MKVPKVLVKGKERAEPIVKNFEWTWTTAVLFSLGFAFFSVITMA
                     VIPSAWLYFAEQKLGWGGLSGTSDWHPMARDFVAANLAVGPFVTVVVIGAIMQNWRRK
                     LRGGTADRPTGGYR"
     gene            3254..3652
                     /locus_tag="VIH70_12005"
     CDS             3254..3652
                     /locus_tag="VIH70_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY8117131.1"
                     /translation="MPFTKTEVDVHTDYVVETVDPDWLAKGVKSPKHLMLDPDNCILC
                     RACEDVCPWNCIFMMSTDIVQDAEDDAVGAEVHTAYSVFVVDDNACTRCSVCVDRCPT
                     DTLYYARLPEASRGQRTMASVPESQRAEAG"
     gene            3750..4451
                     /locus_tag="VIH70_12010"
     CDS             3750..4451
                     /locus_tag="VIH70_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY8117132.1"
                     /translation="MFRPGSIFRRGYRDTSRDRALATMNNVLYHLHPVKMKRHGLKLT
                     YTFCLGGLSFFLFILLTITGIYLMFFYRPAAELGSAAAYTDMQNIRTSVFFGDLVRNL
                     HRWGAHLMVFSVTLHMARVFYTGSYKPPREFNWVVGVLLLFLTLGLSFTGYLLPWDQL
                     AVWAVTVGTSLAGYSPLIAKQANFLLLGGVVVGPDTLLRWYVLHVLALPFVLVIFLSV
                     HFWRIRKDGGISGPM"
BASE COUNT          746 a         1536 c         1515 g          658 t
ORIGIN      
        1 cacgaccagc cccatccaga cgaacaccgg catccggaac agcgtcatgc ccggcgcccg
       61 catgttcagg atcgtcacgg cgaggttgat ggccgagacc gtcgacgcga tcccggcgat
      121 caggagcccg atcgcgtaga acgtcatccc ccgcgtgggg tcgatcacgg agttcggcgc
      181 gtaaccgaac caccccccgt tcggcgcccc gcccaggaac cagctcgagt agatgaacag
      241 cccggcgaac aggaacgccc agtagctgaa ggcgttcagg cgcgggaacg cgacgtcgcg
      301 cgcgccgatc atgagcggca gcaggtagtt gaagaacgcc gccgacagcg gcatcaccac
      361 caggaagatc atcgtgatgc cgtgcatcgt gaacagctcg ttgtacacgg cgggctcgag
      421 gaagctctgg tcgggccgcg cgagctggac gcggatcagg agtgcctcgg acccgccgat
      481 caggaagaac gcgaaggcgg tgatcccgta caggaccccg atcttcttgt ggtcgatggt
      541 cgtgaaccag ctccacaacc ccgtcgtcgc gtgcggtcgc cggatcagcc cgccgggccg
      601 ctccgcgggg acgctcacgc tcgccatggg gtcctcacct cactccagcg tattgaggaa
      661 cgcgacgacc tgatcgacct ggtcgggcgt caggccgtag tccggcatga acgaacccgc
      721 ctcgaccgcg cgcgggttcg cgatccaccg tgccaggttc tcgtcgttgg tctcgagcgt
      781 gcagccacgg aagcactccc ggctcatcag gtgcgtgagg ttcggcgcgc cgttggcgag
      841 cgcgggctga ccgttggccg tctcgagccc ggcgacggcg tggcaggcgg tgcacgtctg
      901 ctggaagatc tccttcccgg cgacggcggg acccgacacg tccggcgacg ccggcgcctt
      961 ctgctcggcc acccagcgct cgaaatccgc gggcgtgtgc gcgaccaccc ggaacctcat
     1021 gtacgcgtga gacaggccgc agaactcctt gcactgtccc tggtaggtac cgggctcgtc
     1081 ggcctggatc gtcaggtggt tcgtcaggtc cggcacgacg tcctgggtgc ccgcgagctg
     1141 cggcacccag aaggagtgga tcaccgcggc gccgatcccg gccggcggct ggccgtccgg
     1201 cggcccggtc tggcacgcgt tcggcgcggg tgggccgccc tggctcggga cgggtgttcc
     1261 gtagccgaac cccacggcgc acagggtcag gtagacgggc cgccccgtgg ggatatgcag
     1321 ctcgttcgcg gtcaggatct tctggcccgg ataatcgaac tcccaccacc actggtgcgc
     1381 gaggacgttg acctcgagca cgtccccggt cggttcggcc gccaggtccc agatcgtcgc
     1441 gatcgtcggc acggccacgc cggccaggat cagcgtcggc aggatcgtcc acgcgatctc
     1501 gagccgggtg ttgccgtgga cctgcgcggg gatgcgtggg gttcggttct tacggtgtcg
     1561 gtaccggaag acaaacagga ccagcaggcc ctcgacgatg acgaagatcg cgaccgcgac
     1621 ccagaacacc gggacgaaca ggtcgaagga cttctgcgcg tactgaccct tgggctccag
     1681 cgccgattgg gtggcgttcg gcgcacaggc cgcggccacg agggccaggg ccaccaggcc
     1741 caggaccacg accgttctcc tgcgcaccgt tccggtctac ctccgcgtgg acctcggacg
     1801 ggcaccgagg cccggcgcac tgtagcaccg ttccccgcgc tcgcggacgc acggctccgc
     1861 gctgtgagta cccacgatcg gcacgccgcg cacctcagat cggcgggctg aaatcgtcga
     1921 tcgcgcggaa cacgggactc agcatccgcg tccacaggtt cgtcgcgacc agcacgccga
     1981 tcgcgatcat caccacaccc gagaccccgg cgatccactg gtaattgcgc ttgacgaacg
     2041 cgagggcgcc catgagcttg cggatcccca ccccaaccaa caggaacgga aggcccagcc
     2101 ccatcgagta gacgaacagg aggagggcgc cgcggagcga gccgccctgc gagcccgcga
     2161 tcgccagcac gcccgcgagg accgggccga tgcacggcgt ccaccccgct ccgaacgcga
     2221 cgccgagcgg gaacgcccac gcgcggcccg gcctggctcg ttcgagcagc ggccggcgtt
     2281 cgaggaacag ggacggcgac ccgagacgga gcgcgtacag cagcatcagg accccgaacg
     2341 cgatgatgat cgcgcccgag atccggatgc ccgccgtcga ccgcagcagg cgcgtcatcg
     2401 cgccggtgaa ggcgccgagc gccgagaaca ccgcggcgaa gccggcgatg aacagcaaga
     2461 tcggcaccac cggctggcgt tcctcgcgca cctcgccgcc ggccacgaac gagaggtagc
     2521 cggggaccag cgggaacacg cacggcgacg cgaacgagat caccccggcg acgaacgcga
     2581 gacccggggc ccaccacgac cccagaccgt cctggaccca gtcggcgagc accccggtca
     2641 ccgcgccagt ctagggtcgg cgcccgcgcg agggttcggc gccgcaggcg cggtgggttg
     2701 cggtcccatg tccggtcctg cgatagtgac gacggccttg tgaaccgatt cacaagcagc
     2761 gagccgggag cgcgaccgcg gggggtcgcg gagcagcgtg actataatcc gacccggtcc
     2821 aggcacgaac gaggcccggc gcacgaagac gtggggaacg agaggacggt cgtggcgacg
     2881 gaggccgtat gaaggtcccg aaggtgctcg tcaaggggaa ggaacgcgcg gagccgatcg
     2941 tcaagaactt cgaatggacc tggacgaccg cggtcctgtt ctccctgggg ttcgccttct
     3001 tcagcgtcat cacgatggcc gtgatcccct cggcatggct ctacttcgcc gagcagaagc
     3061 tcgggtgggg cggcctgtcg gggacctcgg actggcatcc gatggcccgg gacttcgtcg
     3121 ccgcgaacct cgcggtcggc ccgttcgtca cggtggtcgt gatcggcgcg atcatgcaga
     3181 actggcggcg caagctccgg ggcggcaccg ccgaccgtcc caccggcggg taccgatgag
     3241 ggagtcctag gcgatgccgt tcaccaagac cgaggtcgac gtccacaccg actacgtcgt
     3301 cgagaccgtc gaccccgatt ggctcgcgaa gggcgtcaag tcgcccaagc acctgatgct
     3361 cgacccggac aactgcatcc tgtgtcgagc gtgcgaggac gtctgcccgt ggaactgcat
     3421 cttcatgatg tcgaccgaca tcgtccagga cgcggaggat gacgccgtcg gggccgaggt
     3481 ccacacggcg tactcggtgt tcgtcgtcga cgacaacgcg tgcacgcggt gctcggtgtg
     3541 cgtcgaccgg tgccccaccg acacgctgta ctacgcgcgg ctgccggagg cctcgcgcgg
     3601 gcagcgaacg atggcctcgg tacccgagtc gcagcgcgcc gaggccggct aggcggggga
     3661 aggatgaggg ggacgacgtg gcgacgctga agctgccgac gcccggcgag gccctcgacc
     3721 gcgtccgcga gaccgaagtc tggagatcga tgttccgccc cggctcgatc ttccgtcgcg
     3781 gctaccgaga cacctcccgc gaccgcgccc tcgcgacgat gaacaacgtg ctctatcacc
     3841 tgcacccggt gaagatgaag cggcacgggc tgaagctcac ctacacgttc tgcctgggtg
     3901 gcctctcgtt cttcctgttc atcctgctga cgatcacggg catctacctg atgttcttct
     3961 atcgccccgc cgccgagctc ggttcggccg cggcctacac ggacatgcag aacatccgca
     4021 cctcggtgtt cttcggcgac ctggttcgca acctgcaccg ttggggcgcg cacctgatgg
     4081 tgttcagcgt gacccttcac atggcccggg tcttctacac cggctcgtac aagccgcccc
     4141 gagagttcaa ctgggtggtg ggcgtgctgc tgctgttcct cacgctgggg ctgtcgttca
     4201 ccggctacct gctgccgtgg gaccagctgg cggtgtgggc ggtcacggtg gggacctcgc
     4261 tcgccggcta ctcgccgctg atcgcgaagc aggccaactt cctgctgctc ggcggggtgg
     4321 tcgtgggtcc ggacaccctg ctcaggtggt acgtcttgca cgtgctcgct ctgccgttcg
     4381 tcctggtgat cttcctctcg gtccacttct ggcggatccg caaggacggc ggcatctcgg
     4441 gaccgatgta gcgga
//
LOCUS       DATBRK010000406         7665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1533471, whole genome shotgun sequence.
ACCESSION   DATBRK010000406 DATBRK010000000
VERSION     DATBRK010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7665
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533471"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..808
                     /locus_tag="VIH70_12015"
     CDS             44..808
                     /locus_tag="VIH70_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8117133.1"
                     /translation="MSATNEIAPAHARTGVAARAFGLTKTYGEGDATVRALDDVSVEF
                     ERARFSAIMGPSGSGKSTLLHCMAGLDKPTSGQVLIGDVDLTTLSEKDLTLLRRKSVG
                     FVFQAYNLVPTLTASENITLPLDIAGEEPDRAWFDEVVDTVRIRDRLSHRPAELSGGQ
                     QQRVAGARALVSRPEIVFADEPTGNLDSRASQEILTFLRSAVKDHGQTIVMVTHDASA
                     ASFADRIVFLGDGRVVDEMVEPTTERILDRLKSLEG"
     gene            811..3345
                     /locus_tag="VIH70_12020"
     CDS             811..3345
                     /locus_tag="VIH70_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8117134.1"
                     /translation="MFRAAIRSLLARKVRLLLTGLSVVLGVGFMAGTFVLTDTMTRAF
                     NDLFDNAYSSIDVLVRSENAFTAQTSSLEEREPMPESVLDQVRDVPGVALAEGDVLGY
                     AQIVDPATGKVIGTFGPPTAASAWNQLNGFRLKPGGSPPNGPDQVVIDAGTAESHDIQ
                     VGDRVEIVFEGPPGEFDVVGLAGYGDANSLGGATWALFDLPTAQRVLGKEGELDSVSV
                     VAQQGASGVELQRRIAEVLPKGVEAVTAATVTSEAQDQISTGLGFLRTAFLVFAFVAL
                     FVGAFIIFNTFAIIVAQRTRELALFRALGASGRQVMTSVLVEATVIGIVSSLLGVLAG
                     IGIAVLLKGALSATGLKIPSSGTVILTRTIVVSVVVGTLVTVIAALVPARRAARVAPI
                     EALREAQDRPGRSLRFRLISGLVVLALGVVPLVYGLFGTPKNALQLVGLGVALTFVGV
                     AMLTPLIARPIAGTIGLPIRKTGVPGKLGGENSMRNPRRTAATASALMIGLGLVVFVA
                     VFGASAKASTSEVLDRTLKADFILTSPTLTGFSTAAAEDLRGIQDVETVSQVRQGEVH
                     IKEGTSFVTGLDPTTFPEVSELGVNDGSLGELQSANTIAVFDDTAADNGWRVGDTITV
                     EFPATGKVDLDVVALYSENGLIGDYAVSLDTFDANVAQQLDLFVLVRATEGADLSAVR
                     ADIETALKPYPNVEVLDQAEFRDKYATFINQILNLVTALLLFAVIIALFGVMNTLYLS
                     IYERTRELGLLRAVGLTRRQTRSMVRWEAAIISVMGALLGVVIGIAFGWALQQALEPK
                     GFTQLGIPGGQLAFYVVFAALLGVFFAIFPARRAAKLNVLDAISYE"
     gene            3406..5334
                     /gene="thrS"
                     /locus_tag="VIH70_12025"
     CDS             3406..5334
                     /gene="thrS"
                     /locus_tag="VIH70_12025"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY8117135.1"
                     /translation="MPKIELAGGSTTDLPEGEPVGTVLPPEAIAARVDGELVDLSFVP
                     VTDGKVEPVLPDDPDGLHVLRHSAAHVLAQAVCDLWPGTRYAIGPPIEDGFYYDLELP
                     GQVSEGDLARIEDRMREIVDADQPFVREEIPRAEALERFVDQPYKREIIESLEEGEVP
                     AGDTVTIYRNDGWADLCLGPHVPSTGRLKAFKLMKVAGAYWRGDEARPMLTRIYGTAW
                     STPKELRSYLHRLEEAEKRDHRRLGRELDLFSFPEELGPGLAVWHPKGGVFRKQLEDY
                     VRDLHLERGYDPVVTPNLARRELWDTSGHTEKYAELMYPEMRHEGDPAYFVKPMNCPL
                     HVLVYKSQIRSYRELPIRLSELGGVYRYERLGVVHGLLRARGFTQDDSHIICREDQLV
                     DEILGVFELTLEIHRTFGFTEPVVTLSTLPGESIVTPEMAERATGALRDALERSGLEF
                     RVAEGEGSFYGPKVDFHFRDAIGRLWQLTTVQCDFGLPERFQMEYVGEDNRRHRPVMV
                     HRAILGSIERFSAVLIEHYGGAFPLWLSPEQVRLVPVADRHLEHARELASLLRALGLR
                     PAVDESGESVGKKVRAAQLAKAPYVLVIGDKEIESGELTVRDREGIETKAVPFDDFAA
                     ALVDEAASKRLSQSGFGG"
     gene            5357..5851
                     /locus_tag="VIH70_12030"
     CDS             5357..5851
                     /locus_tag="VIH70_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY8117136.1"
                     /translation="MDRIWSPWRMAYIQAAKQQDDDAGCIFCDLPAEGDDERTKILVR
                     GDLAFVILNSFPYNPGHLMVAPFRHVGDLGELETAEFAEVDALLVRSIRALEAETEPH
                     GFNLGMNLGRVAGAGIPNHVHWHVVPRWNGDTNFMPVVGETRVLPELLEETYARLLPR
                     FESA"
     gene            5848..6681
                     /locus_tag="VIH70_12035"
     CDS             5848..6681
                     /locus_tag="VIH70_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8117137.1"
                     /translation="MSEGLTRAESGVIEFDGGTLAWESAGEGPGVVFLHPGLWDGRVW
                     DEQFGVFSRTYRVLRFDFRGYGHSSRPEPGRAYSHVDDLAAVMDAARIERAALVGCSM
                     GGSTAIDFAIVHPSRVWALVLAGSGLNAFDDRLTPQEEAELERLSAPVAEAMEAGDIE
                     RAERARLRIWAPLGTEDEAGRRIREIAFDNIHEMTMDESARRDISPPAIERLEQIKAP
                     TLVLPADKDPLVYRRLSAILAERIPDARLVQIPETDHVVNMRRPDEFDRVVLGFLDEV
                     L"
     gene            6730..7092
                     /locus_tag="VIH70_12040"
     CDS             6730..7092
                     /locus_tag="VIH70_12040"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY8117138.1"
                     /translation="MALGAFGSHALGSKLPPDRSAAFETGVRYQMWHALALFTVVIVD
                     AWTPSAESPAFYLTERGGAPLLVAAGWFFVTGVVLFSGSLYALAVTGRRAWGAFTPFG
                     GACLLLGWALLLFAIVLK"
     gene            complement(7099..>7665)
                     /gene="fusA"
                     /locus_tag="VIH70_12045"
     CDS             complement(7099..>7665)
                     /gene="fusA"
                     /locus_tag="VIH70_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8117139.1"
                     /translation="IEPLPRGEGFEFVDKIFGGAIPNTFIGSVEKGIVKTMQSGVISG
                     NPMVDIRCTLVDGKFHSVDSSDMAFQLAGALALKEAAAAAGVVLLEPIVEVDVVVPES
                     HTGDIMGDLNAKRAKIQGVESAGAGKQRVKALVPQAEVARYSIDLRSMTGGRAAFTMH
                     FSHYEEVPSHLADRIIAAAQQEKEEARK"
BASE COUNT         1138 a         2553 c         2728 g         1246 t
ORIGIN      
        1 ggttcggtaa cgttttcgga cgatcgtcct gagggggagc acgatgtccg caaccaacga
       61 gatcgcgccg gcccatgccc gcacgggcgt ggcggctcgc gcgttcggcc tgaccaagac
      121 gtacggcgaa ggcgatgcga ccgtccgggc gctcgacgac gtctccgtgg agttcgagcg
      181 cgcccggttc tcggcgatca tgggtccgtc ggggtcggga aaatcgaccc tgcttcactg
      241 catggctggc ctcgacaagc caacctccgg gcaggtcctg atcggcgacg tcgatctgac
      301 cacactctcc gagaaggacc tgaccctgct gcggcgcaag agcgtcgggt tcgtgttcca
      361 ggcctacaac ctggtgccca cgctcacggc gtccgagaac atcaccctcc cgctggatat
      421 cgccggtgag gagccggaca gggcgtggtt cgacgaggtg gtcgataccg tgaggatccg
      481 cgaccggctc agccaccggc cggccgagct ctcgggcggc cagcagcagc gggtggccgg
      541 cgctcgggcc ctcgtcagcc ggccggagat cgtgttcgcg gacgagccga cgggcaacct
      601 ggactcgagg gcgagccagg agatcctgac gttcctgcgg agcgccgtga aggaccacgg
      661 gcagacgatc gtgatggtga cgcacgacgc ctcggcggcc agcttcgccg accggatcgt
      721 gttcctgggc gacggcaggg tcgtcgacga gatggtggag ccgaccacag aacggatcct
      781 ggaccgactg aagtcgttgg agggctgagc gtgttccgcg cggcgatccg cagcctcctc
      841 gcccggaagg ttcgtctgct cctgacgggc ctgtcggtgg tgttgggcgt gggcttcatg
      901 gccgggacct tcgtcctcac cgacacgatg accagggcgt tcaacgacct gttcgacaac
      961 gcgtactcgt cgatcgacgt gctggtgcgc agcgagaacg cgttcaccgc gcagaccagc
     1021 agcctcgagg agcgcgagcc gatgcccgaa tcggtgctgg accaggtccg cgatgtcccc
     1081 ggcgtcgcgc tggccgaggg agacgtcctc ggatacgccc agatcgtcga tccggcgaca
     1141 ggcaaggtga tcgggacgtt cgggcccccg acggccgcgt ccgcgtggaa ccagctgaac
     1201 gggttcaggc tcaagccggg cgggtccccg ccgaacggcc ccgaccaggt cgtgatcgac
     1261 gcggggacgg ccgagtcgca cgacatccag gtcggcgacc gcgtcgagat cgtgttcgag
     1321 ggtccgcccg gcgagttcga cgtggtaggc ctcgccgggt acggcgacgc gaacagcctg
     1381 ggcggggcga cctgggcgct gttcgacctg ccgaccgcgc agcgggtgct gggcaaggag
     1441 ggcgagctcg actccgtctc ggtcgtggca cagcaggggg cctcgggtgt cgagctccag
     1501 cgtcggatcg cggaggtgct gccgaagggc gtcgaggccg tcacggccgc caccgtgacg
     1561 tcggaggcgc aggatcagat cagcaccggc ctggggttcc tccgcacggc cttcctggtc
     1621 ttcgcgttcg tcgccctgtt cgtcggcgcc ttcatcatct tcaacacgtt cgcgatcatc
     1681 gtcgcccagc gaacgcgcga gctggccctg ttccgcgcgc tcggtgccag cgggcgccag
     1741 gtgatgacct cggtgctcgt cgaggcaacg gtgatcggca tcgtgtcctc gctgctcggc
     1801 gtgctggccg gcatcgggat cgcggtcctc ctcaagggcg ccctgagcgc gaccgggctc
     1861 aagatcccgt cgtccgggac ggtgatcctg acccggacga tcgtcgtctc ggtggtggtc
     1921 ggaacactcg tcacggtgat cgcggctctg gtcccggcgc gacgcgccgc ccgggtcgcg
     1981 ccgatcgagg cgctgcgcga ggcgcaggac cggcccggtc gctcgctgcg gttccgcctg
     2041 atctccggcc tggtcgtgct ggcactcggg gtcgtgccgc tcgtgtacgg gctgttcggc
     2101 acgccgaaga acgcgctcca gctcgtgggc ctgggcgtcg cgctgacgtt cgttggcgtc
     2161 gcgatgctca cgccgctgat cgcgcgtccg atcgccggca ccatcggcct gccgatccgc
     2221 aagaccggag tgcccggcaa gctgggcggc gagaactcga tgcggaaccc gcgccggacg
     2281 gcggcgacgg cctccgccct gatgatcggg ctcggcctgg tcgtgttcgt cgcggtgttc
     2341 ggagcatcgg ccaaggcgtc caccagcgag gtcctcgacc gaacgctgaa ggccgacttc
     2401 atcttgacct cgccgaccct taccggcttc agcaccgccg ccgcggagga cctgcggggg
     2461 atccaggacg tggagacggt gtcgcaggtc cggcagggcg aggtgcacat caaggaaggg
     2521 acctcgttcg tcacgggtct ggaccccacg accttccccg aggtatccga gctcggggtg
     2581 aacgacggct cgctcgggga gctccagagc gcgaacacga tcgccgtgtt cgacgacacc
     2641 gccgcggata acggctggcg cgtgggggac acgatcacgg tcgagttccc cgcgaccggg
     2701 aaggtggacc tggacgtcgt cgcgctctat tcggagaacg ggctgatcgg cgactacgcg
     2761 gtctcgctcg acacgttcga cgccaacgtc gcccagcagc tcgacctctt cgtgttggtc
     2821 agggccaccg agggcgccga cctgtctgcc gtccgggcgg acatcgagac cgcgctgaag
     2881 ccgtacccga acgtcgaggt gctggaccag gcggagttcc gcgacaagta cgcgacgttc
     2941 atcaaccaga tcctgaacct ggtcacggcg ctgctgctgt tcgcggtgat catcgccctg
     3001 ttcggggtga tgaacacgct gtacctgtcg atctacgagc ggacccggga gctcgggttg
     3061 ctgcgggccg tgggcctgac ccggcggcag acccgctcga tggtccgttg ggaggccgcg
     3121 atcatctcgg tcatgggagc cctgctcggc gtcgtgatcg gtatcgcgtt cgggtgggcc
     3181 ctgcagcagg ccctcgagcc caaggggttc acccagctcg ggatccccgg tggtcagctg
     3241 gcgttctacg tggtgttcgc ggccctgctc ggagtgttct tcgcgatctt cccggcccga
     3301 cgcgcggcca agctcaacgt gctcgacgcg atctcgtacg agtaggacga gcccgcggcc
     3361 cgaacgggcg agcggcgctc gagcggtgga tatcatggcc gtcccatgcc gaagatcgag
     3421 ctcgccggcg gttcgaccac cgacctcccc gagggggaac cggtcggcac cgtcctgccg
     3481 cccgaggcga tcgcggcccg cgtggacggc gagctggtcg acctctcctt cgtgcccgtt
     3541 accgacggga aggtagagcc ggtcctgccg gacgatcctg acggcctgca cgtcctgcgg
     3601 cattcggcgg cgcacgtgtt ggcgcaggcc gtctgcgacc tgtggcccgg cactcgctac
     3661 gcgatcggcc cgccgatcga ggacggcttc tactacgacc tcgagctccc ggggcaggtc
     3721 tccgagggcg acctcgcgag gatcgaggac cggatgcgcg agatcgtcga cgccgaccag
     3781 ccgttcgtgc gcgaggagat cccgcgcgcg gaagctctgg agcgcttcgt cgaccagccg
     3841 tacaagcggg agatcatcga gtcgctcgag gaaggcgagg tcccggccgg cgacacggtc
     3901 accatctatc gcaacgacgg atgggcggac ctgtgcctgg gtccccacgt gccctccact
     3961 gggcggctca aggcgttcaa gctgatgaag gtcgcgggcg cctactggcg cggggacgag
     4021 gcgcggccga tgctcacccg gatctacggg acggcgtggt ccacgccgaa ggagctgcgc
     4081 tcgtacctgc accgcctgga ggaggccgag aagcgggatc accgccgcct cgggcgcgag
     4141 ctcgacctgt tctccttccc cgaggagctc ggcccgggcc tcgccgtctg gcatccgaag
     4201 ggcggggtgt tccgcaagca gctcgaggac tacgtgcgcg acctgcacct cgagcgcggc
     4261 tacgaccccg tcgtcacgcc gaacctcgcg cgccgcgagc tgtgggacac ctcggggcac
     4321 acggagaagt acgcggaact gatgtacccg gagatgcggc acgagggcga ccccgcctac
     4381 ttcgtcaagc cgatgaactg ccccctccac gtgctcgtgt acaagtcgca gatccggtcg
     4441 taccgcgagc tgccgatccg gctgtcggag ctcgggggtg tctaccggta cgagcggctc
     4501 ggcgtcgtgc acgggctcct gcgggcgcgc gggttcaccc aggacgactc gcacatcatc
     4561 tgtcgcgagg atcagctggt ggacgagatc ctcggcgtgt tcgagctcac gctcgagatc
     4621 catcgcacct tcgggttcac cgaaccggtg gtcacgctct ccacgctgcc gggcgagtcg
     4681 atcgtgacgc ccgagatggc ggagcgcgcg accggcgccc tgcgcgatgc gctcgagcgc
     4741 agcgggctcg agttccgggt cgccgagggc gagggatcgt tctacgggcc caaggtggac
     4801 ttccacttcc gggacgcgat cgggcgcctg tggcagctca ccaccgtgca gtgcgacttc
     4861 ggcctgcccg aacggttcca gatggagtac gtcggcgagg acaaccggcg tcaccgcccg
     4921 gtgatggtcc atcgcgcgat cctcgggtcg atcgagcgct tcagcgcggt gctgatcgag
     4981 cactacgggg gcgcgttccc gctctggctc tcgccggagc aggtgcgcct ggtgccggtc
     5041 gccgaccgcc acctcgagca cgcgcgggag ctcgcctcgc tcctgcgggc actcgggctg
     5101 cgccccgccg tggacgagag cggggagagc gtcggcaaga aggtgcgcgc ggcgcagctc
     5161 gcgaaggccc cctacgtgct cgtgatcggc gacaaggaga tcgagtccgg cgagctcacc
     5221 gtgcgcgatc gggagggcat cgagacaaag gccgtgccgt tcgacgactt cgcggccgcg
     5281 ctcgtcgacg aggcggcatc caagcgcctg tcgcagtcgg ggttcggcgg ctgaggcgag
     5341 gacgccgggg gacggcgtgg atcgcatctg gtcgccgtgg cggatggcgt acatccaggc
     5401 agccaagcag caggacgacg acgccgggtg catcttctgc gacctgccgg ccgaggggga
     5461 cgacgagcgg accaagatcc tcgtccgcgg cgatctcgcg ttcgtgatcc tgaactcgtt
     5521 cccgtacaac ccggggcatc tgatggtcgc cccatttcgg cacgtcggcg accttggcga
     5581 gctcgagacc gcggagttcg ccgaggtcga cgcgctgctc gtgcgctcga tccgcgcgct
     5641 cgaggcggag acggagccgc acggcttcaa cctcgggatg aacctcgggc gagtggccgg
     5701 agccggcatc ccgaaccacg tgcactggca cgtcgtgccc cgctggaacg gcgataccaa
     5761 cttcatgccg gtcgtcggcg agacccgcgt cctgcccgag ctgctcgagg agacgtacgc
     5821 gcggctcctc ccgcgcttcg agagcgcgtg agcgagggtt tgacgcgcgc ggagagcggc
     5881 gtgatcgagt tcgacggggg aaccctcgca tgggagtccg ctggggaggg ccccggcgtg
     5941 gtgttcctgc atccggggct gtgggacggc cgggtctggg acgagcagtt cggcgtgttc
     6001 tcgaggacct accgggtcct acgcttcgac ttccgcggct acggccactc cagccgcccg
     6061 gagcccggga gggcctattc acacgtcgac gacctcgcgg ccgtcatgga tgccgcgaga
     6121 atcgagcgag cggcgctcgt cgggtgctcg atggggggat caacggccat cgatttcgcc
     6181 atcgtgcatc cctcgcgagt atgggctctc gtgctcgcgg gctcgggttt gaacgcgttc
     6241 gacgatcgac tgaccccaca agaggaggcg gagctcgaac gtctgagcgc gccggtcgcc
     6301 gaggcgatgg aggcgggcga catcgagcgg gcggagcgag cacggcttcg catctgggcc
     6361 ccgctcggaa cggaggacga ggccgggagg cggatcaggg agatcgcgtt cgacaacatt
     6421 cacgagatga cgatggacga gtccgcgagg cgcgatatct cgcctccggc gatcgagcga
     6481 ctcgagcaga tcaaggctcc cacgctcgtc ctgcccgccg acaaggatcc gctcgtgtac
     6541 cgaaggttga gtgcgatcct cgccgagcgc atccccgatg ctcggctcgt acagatcccg
     6601 gaaacggacc atgtcgtcaa catgcgacga cccgacgagt tcgatcgggt cgtcctcggg
     6661 ttcctggacg aggtgctctg agcgacggag acgttcattg tcctcggcgg caccttcggc
     6721 ctgatcgggg tggcactcgg cgcgttcggc tcacacgccc tggggtcgaa gctacccccc
     6781 gaccgatccg cggcgttcga gacgggtgtg cggtatcaga tgtggcacgc gctcgcgctg
     6841 ttcacggtcg tgatcgtgga tgcgtggacg ccgagcgcgg aatcccccgc cttctacttg
     6901 accgagcgtg gtggggcacc gctcctggtt gcagccggat ggttcttcgt gaccggggtg
     6961 gtgctgttct caggcagtct gtacgcgctc gcggtgaccg gccgtcgagc gtggggagcg
     7021 ttcaccccgt tcgggggagc ctgtctgctg ttgggttggg cgttgctcct gttcgcgatc
     7081 gtcctgaagt gaggagtcct acttcctcgc ctcttccttc tcctgctgcg ccgcggcgat
     7141 gatcctgtcc gcgaggtgcg acggcacctc ctcgtagtgg gagaagtgca tcgtgaacgc
     7201 cgcccgcccc ccggtcatcg aacgcagatc gatcgagtag cgcgcgacct ctgcctgcgg
     7261 aacgagcgcc ttgacgcgtt gcttgccggc gcccgccgat tcgacgccct ggatcttggc
     7321 gcgcttggcg ttgaggtcgc ccatgatgtc gccggtgtgg gattcgggga cgacgacgtc
     7381 gacctcgacg atcggctcga gcaggaccac cccggccgcg gccgccgcct ccttcagcgc
     7441 gagtgcaccc gccagctgga acgccatgtc ggacgagtcc acgctgtgga acttgccgtc
     7501 gacgagcgtg caccggatgt cgaccatggg gttcccgctg atcacgcccg actgcatcgt
     7561 cttgacgatg cccttctcga ccgagccgat gaacgtgttc gggatcgcgc ccccgaagat
     7621 cttgtcgacg aactcgaacc cctcgccgcg ggggaggggc tcgat
//
LOCUS       DATBRK010000407         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2689535, whole genome shotgun sequence.
ACCESSION   DATBRK010000407 DATBRK010000000
VERSION     DATBRK010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2689535"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VIH70_12050"
     CDS             <1..543
                     /locus_tag="VIH70_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8117140.1"
                     /translation="LVFSPLVIPYLVFGISLLLLFTLADKILTQSFGFYFGLGLHAVV
                     IGHVVVALPYTILTIMPLLERLSVSLDEAAKDLGASPRETFRRVTLPLLMPALISAFL
                     VAFTLSFDEFAIASFLAGTQQTWPVYLFAQLRVPSLLPQLIAVSSVVFIASMLVVVSA
                     EIGRRIAERRYGREYAARLV"
     gene            578..1993
                     /locus_tag="VIH70_12055"
     CDS             578..1993
                     /locus_tag="VIH70_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8117141.1"
                     /translation="MPLPATIAELRGTGFPDRSVKQELRENLLGRLGSGVSLFPQVVG
                     FEDSVLPVLERGLLAGHDLVMLGERGQAKTRLIRQIVDLLDELTPAIAGCEVNDHPYH
                     PICAACRALLAEDGDAAAIRWIGREDRYAEKLATPDTSVADLIGDVDPIRVAEGRYLA
                     DELTIHYGMIPRTNRGIVAINELPDLPERIQVALFNILEERDVQIRGYRIRLPLDLLL
                     VATANPDDYTHRGRIVSPLKDRFGTQVRTHYPESLEDEIAIMEQESRAWESDVPVRVP
                     VYLEEILAAFTAELRRSPHVNHRSGVSVRYSIGNLETLSAAAIRRAARTGEPEAVPRV
                     CDLPAVVEAGLGRVEFDTIEEGREEEILSRALKTAELQVFRRRLSGFDFQPILSRFAQ
                     GFEAETSDLLPASELLAQFGELPGLSKLLQRLGVEEESPGVAASALEFALEGLHLSRR
                     LNKDLEARAGRSRYRSAQPDE"
     gene            2033..>2305
                     /locus_tag="VIH70_12060"
     CDS             2033..>2305
                     /locus_tag="VIH70_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117142.1"
                     /translation="MTEVHRYVGFVVVGIFAIGWLFGLVLWISRREAGDWFWRWLVAA
                     QTVAILQALIGVILLIAGKRPTTGLHYVYGFGPLVVFAIGHVLGREE"
BASE COUNT          311 a          805 c          803 g          386 t
ORIGIN      
        1 ctggtcttct cgccgctcgt gatcccgtac ctggtcttcg ggatctcgct gctcctgctg
       61 ttcacgctcg ccgacaagat cctgacccag tcgttcggct tctacttcgg cctggggctc
      121 cacgcggtcg tgatcggtca cgtggtcgtc gcgctgccgt acacgatcct cacgatcatg
      181 ccgctcctgg aacgcctcag cgtgtcgctc gacgaggcgg cgaaggacct gggggcgagc
      241 ccccgcgaga ccttccgccg cgtcacgctc ccgctcctga tgccggcgct catctctgcg
      301 ttcctcgtgg cgttcaccct gtcgttcgac gagttcgcga tcgcctcctt cctggccggc
      361 acgcaacaga cctggcccgt ctacctgttc gcccagctcc gggtcccgag cctgctcccc
      421 cagctgatcg cggtctcgtc ggtggtgttc atcgcctcga tgctggtcgt ggtctcggcg
      481 gagatcggcc gccgaatcgc cgaacgccgg tacggtcgcg agtacgccgc gcgcctggtc
      541 tgaccgccct atccgaggga gccgttaggc tggggtcgtg ccgctgcccg cgacgatcgc
      601 cgagctccga gggaccgggt tcccggatcg gagcgtcaag caggagctcc gcgagaacct
      661 gctcgggcgg ctcggctcgg gcgtctccct gttcccgcag gtcgtggggt tcgaggactc
      721 ggtgctgccg gttctcgaac gcggactcct ggcgggccac gacctcgtga tgctcggcga
      781 gcgggggcag gccaagacgc gtctgatccg ccagatcgtc gacctcctgg acgagttgac
      841 cccggccatc gcggggtgcg aggtgaacga ccacccgtac cacccgatct gcgcggcctg
      901 ccgggccctg ctcgccgagg acggggatgc ggcggcgatc cgctggatcg ggcgcgagga
      961 ccgctacgcg gagaagctcg cgacgccgga cacgagcgtc gccgacctga tcggcgacgt
     1021 cgatccgatc agggtcgccg aggggcggta cctcgcggac gagctcacga tccactacgg
     1081 gatgatcccg cggaccaacc gagggatcgt cgccatcaat gagctccccg acctgcccga
     1141 acgcatccag gtcgcgctgt tcaacatcct ggaggaacgt gacgtgcaga tccgcggcta
     1201 tcggatccgg ctcccgctcg acctcctgct cgtggccacg gcgaaccccg acgactacac
     1261 gcaccgaggc cggatcgtct cgccgctcaa ggaccggttc ggcacgcagg tacggacgca
     1321 ctatccggag tcgctcgagg acgagatcgc gatcatggaa caagagtcgc gcgcctggga
     1381 gagcgacgtc cccgtccggg tcccggtgta cctcgaggag atcctcgcgg cgttcacggc
     1441 cgagctccgg cgttcgccgc acgtcaacca ccggagcggt gtgagcgtgc ggtactcgat
     1501 cggcaacctc gagacgctct cggcagccgc gatccggcgg gcggcgcgga ccggcgagcc
     1561 cgaggccgtc ccgcgggtgt gcgacctccc ggccgtcgtc gaggcgggtc tcgggcgggt
     1621 cgagttcgac acgatcgagg agggccgcga ggaggagatc ctgtcccggg ccctcaagac
     1681 ggccgagctc caggtgttcc gccggcggct gtcgggcttc gactttcagc cgatcctgtc
     1741 gcggttcgcg caggggttcg aggccgagac ctcggacctg ctgccggcga gcgagctgct
     1801 ggcgcagttc ggcgagctcc ccgggttgtc caagctgctg cagcgtctgg gcgtcgagga
     1861 ggagtcgccc ggcgtcgcgg cctccgcgct ggagttcgcg ctcgagggcc tgcacctatc
     1921 gcgccggctc aacaaggacc tggaggcccg ggcgggccgc agccgatacc ggtcggccca
     1981 acccgacgag tagcgacgcc ccgcggctcg caccccgtag gattcggccg ccgtgaccga
     2041 ggtccaccgc tacgtcgggt tcgtcgtcgt cgggatcttc gcgatcgggt ggctcttcgg
     2101 cctggtcctg tggatctcgc gccgggaagc gggggactgg ttctggcgtt ggctcgtcgc
     2161 ggcgcagacc gtggccatcc ttcaggcgct gatcggcgtg atcctgctga tcgcgggaaa
     2221 gcgacccacc acggggctgc actacgtcta cgggttcggg ccgctggtgg tcttcgcgat
     2281 cggccacgtc ctggggaggg aagag
//
LOCUS       DATBRK010000408         3436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1533565, whole genome shotgun sequence.
ACCESSION   DATBRK010000408 DATBRK010000000
VERSION     DATBRK010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533565"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VIH70_12065"
     CDS             complement(<1..260)
                     /locus_tag="VIH70_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY8117143.1"
                     /translation="MTKYVYDFSEGNRDMKDLLGGKGANLAEMTNMGLPVPPGFTITT
                     EACLTFLAQGGAPPEMLEEAGEHLRRLEDAMGKTLGDPDDPLL"
     gene            complement(346..756)
                     /locus_tag="VIH70_12070"
     CDS             complement(346..756)
                     /locus_tag="VIH70_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117144.1"
                     /translation="MDSILVQTTRSLRALIGNATDGAPAKVIDQAYLALLDQCAAAGR
                     EALRTPSDPIARFDARAAAVLSVAYRPVLDVLCSGRSLRSDDVELAQKSSGWLNLAYE
                     ALLDGDHETVERCMRMASVFVDAGEDSHDTAPPA"
     gene            complement(938..2281)
                     /locus_tag="VIH70_12075"
     CDS             complement(938..2281)
                     /locus_tag="VIH70_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117145.1"
                     /translation="MRLRGAALAAVLGLTLGACTGGADVPGDPASAPETSVEAVPASP
                     EVPSPAPGGPDSAAAAAKRLCEVTQPRPGAGSDVPAEGPTPAVIAQVMRQVAQIRGFD
                     YSERVVAEPVSQAEIGRDFRKFSDITYPEGQYARRSIAWDTIGVIPDGTSLRDAYESY
                     GSSQVIGYYDTVTGELKFTGSDSPSPLERITLAHELTHAIDDQRFGLERLDALGAECK
                     DEDASAAIALVEGNATFFMLRWARTFLTLAEQVQVGIEAAAQDTSTEGVPPFVARLQA
                     WPYDQGMRFIGALEARGGLDEVDEAFVDLPTSTEQIIHPERYPNDAPTPVDVPDLSAE
                     LGPGWEDLDVMSIGEAWLQIALGLRLDGSEAGAAAAGWDGGTYRAWSNGTETAVELST
                     VWDSARDAEEFAAAMTEWVDQGDGTATVLEPEGSAVTVLFASDAAALDVLETAIG"
     gene            complement(2278..2472)
                     /locus_tag="VIH70_12080"
     CDS             complement(2278..2472)
                     /locus_tag="VIH70_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117146.1"
                     /translation="MDEPGRGWVSLYAAAFALMLAAAIALMASSLGSLRSIGLLWISS
                     ALSLLAAGLAVASVLVPRRG"
     gene            complement(2476..>3436)
                     /locus_tag="VIH70_12085"
     CDS             complement(2476..>3436)
                     /locus_tag="VIH70_12085"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY8117147.1"
                     /translation="PRQFNLMFKTHMGPIEDESSESYLRPETAQGMFVDFAIVQQTSR
                     KKVPFGIAQIGKSFRNEITPGNFIFRTREFEQMEMEYFVEPGTDDEWFEYWVNERLQW
                     YVDLGIRKEKLRLRPHDPGELAHYAKAASDVEYEFPFGWQEVEGIANRTDFDLKAHQQ
                     ASGEDLTYFDQERDRRYLPYVVEPAAGVDRVLLTALVDAYREEDAPTAKGDTEKRTYL
                     ALHRDLAPTKVAVLPLSRNERLAPDARGVFDLVKRLWMCDYDDAGSIGRRYRRQDEVG
                     TPFCVTVDFDTLEDRQVTVRERDSMAQDRIPVENLVDYLAERL"
BASE COUNT          492 a         1214 c         1199 g          531 t
ORIGIN      
        1 agcagcgggt cgtcggggtc gccgagcgtc ttgcccatgg cgtcctcgag acggcgaagg
       61 tgctcccccg cctcctcgag catctcgggc ggggcacccc cctgcgcgag gaacgtcagg
      121 cacgcctcgg tggtgatcgt gaagccgggc gggaccggca gtcccatgtt ggtcatctcg
      181 gcgaggttgg cccccttgcc gccgaggagg tccttcatgt cccggttgcc ttcggagaag
      241 tcgtagacgt acttggtcat cagcgatgcc tccgccgcga gatgggttcg gaccggacca
      301 gtctacggac gtgacggagg caaccgcgcg gcgggcgagc ccgggctagg cgggcggagc
      361 ggtgtcgtgg gagtcctcgc ccgcgtccac gaacacggac gccatgcgca tgcagcgctc
      421 gacggtttca tggtcgccgt ccaggagcgc ctcgtaggcg aggttcagcc agccggagct
      481 cttctgcgcg agctcgacgt cgtccgagcg gagcgaacgg cccgagcaca ggacgtcgag
      541 caccggccgg taggccacgc tgaggaccgc ggcggcccgg gcgtcgaacc gggcgatggg
      601 gtcggacggg gtccggagcg cctcgcgccc ggcggcggcg cattggtcga gcagagcgag
      661 gtacgcctgg tcgatcacct tggccggggc gccgtccgtg gcgttgccga tcagggcccg
      721 gaggcttcgc gtggtctgta ccaggatgct gtccatgagc ggagcctagt gtcgtccccc
      781 gcccggcgtc cgcaccatcg gccgggcgac cccgaaatga ggccgatagg cctaggacgt
      841 tcggcgcatc tggaccggat ggcagatccg cctgaggcgc ggatccaccg gagcggtatg
      901 cctcgatgcg catgcctcgg tggcgaacga gtgagcctca gccgatcgcg gtctcgagca
      961 cgtccagcgc cgccgcgtcg ctcgcgaaca gcaccgtgac ggcgctgccc tccggttcga
     1021 ggaccgtggc ggtgccgtcc ccctggtcga cccactcggt catcgcggcc gcgaactcct
     1081 ccgcgtcacg agccgaatcc cagaccgtcg acagctcgac ggccgtctcg gtcccgttcg
     1141 accacgcgcg gtacgtcccg ccgtcccatc ccgcggccgc ggccccggcc tccgagccgt
     1201 cgagccgcag gccgagcgcg atctgcagcc acgcctcgcc gatcgacatc acgtcgaggt
     1261 cctcccatcc cggtcccagc tccgccgaga ggtccggcac gtcgaccggg gtcggcgcgt
     1321 cgttcggata gcgctcgggg tggatgatct gctcggtcga tgtggggagg tcgacgaacg
     1381 cctcgtccac ttcatcgagg ccgccccgtg cctcgagcgc cccgatgaaa cgcatcccct
     1441 ggtcgtacgg ccatgcctgg agccgggcca cgaacggcgg cacgccctcc gtcgacgtgt
     1501 cctgggccgc ggcctcgatc cccacctgca cctgttcggc caacgtgagg aacgtccgcg
     1561 cccaccgcag catgaagaac gtcgcgttgc cctcgaccaa cgcgatcgcg gcggacgcgt
     1621 cctcgtcctt gcactcggcg ccgagcgcgt cgagccgctc cagcccgaac cgctggtcgt
     1681 cgatcgcgtg cgtcagctcg tgggcgagcg tgatgcgctc gaggggtgag ggcgagtcgc
     1741 tccccgtgaa cttcagctcg cccgtcacgg tgtcgtaata gccgatcacc tggctcgagc
     1801 cgtagctctc gtaggcgtcg cggaggctgg tgccgtcggg gatcaccccg atggtgtccc
     1861 acgcgatgct ccgtcgtgcg tactgcccct ccgggtaggt gatgtcggag aacttccgga
     1921 aatcacggcc gatctccgcc tgcgacaccg gctcggccac gacccgctcc gagtagtcga
     1981 acccgcggat ctgcgccacc tgccgcatca cctgcgcgat cacggcgggc gtcggcccct
     2041 cggcggggac gtccgatccg gcgccgggcc tgggttgggt cacctcgcaa agacgcttcg
     2101 cggcggcggc ggccgaatcc ggtccgccgg gcgccggcga gggaacctcc ggggaagccg
     2161 gcaccgcctc gaccgaggtc tcgggggcgg acgcggggtc gccggggacg tcggctccgc
     2221 cggtacaggc cccgagcgtg agcccgagga cggccgccag ggcggctccg cgaagcctca
     2281 tccgcgcctc ggcaccagga cgctcgcgac ggccaggccg gcggcgagga gcgagagggc
     2341 gctcgagatc cataggagtc ctatcgaccg cagggagccg agcgatgagg ccatgagcgc
     2401 gatcgccgcc gcgagcatca gcgcgaaggc ggccgcatac agactcaccc agcccctccc
     2461 cggctcgtcc atcgtctaga ggcgttcggc gaggtagtcc acgaggttct cgacggggat
     2521 ccggtcctgc gccatcgagt cccgttcgcg gacggtgacc tggcggtcct ccagcgtgtc
     2581 gaagtccacg gtcacgcaga acggcgtgcc cacctcgtcc tggcggcggt accggcgacc
     2641 gatcgagccg gcatcgtcgt agtcgcacat ccacaggcgc ttgacgaggt cgaacacgcc
     2701 cctcgcatcc ggcgcgagcc gttcgttgcg cgagagcggc agcacggcga ccttggtcgg
     2761 cgccagatcc cggtgcaggg cgaggtacgt gcgcttctcg gtgtcgccct tggcggtcgg
     2821 cgcgtcctct tcccggtagg cgtccacgag cgcggtgagc agcacgcgat cgacaccggc
     2881 ggcgggctcg accacgtagg ggaggtagcg ccgatcccgc tcctggtcga agtaggtcag
     2941 gtcctcgccg ctcgcctgct gatgcgcctt gaggtcgaag tcggtgcggt tcgcgatgcc
     3001 ctcgacctcc tgccacccga acgggaactc gtactccacg tcggaggcgg ccttcgcgta
     3061 gtgcgccagc tcgccgggat cgtgcgggcg caggcggagc ttctccttgc ggatcccgag
     3121 gtcgacgtac cactgcagcc gctcgttcac ccagtactcg aaccattcgt cgtcggtgcc
     3181 gggctcgacg aagtactcca tctccatctg ctcgaactcg cgcgtacgga agatgaagtt
     3241 gcccggcgtg atctcgttgc ggaacgactt gccgatctgc gcgatcccga acggaacctt
     3301 cttgcgcgag gtctgctgga cgatcgcgaa gtcgacgaac atcccctgcg ccgtctcggg
     3361 tcggaggtag gactcgctcg actcgtcctc gatggggccc atgtgcgtct tgaacatcag
     3421 gttgaactgc cggggc
//
LOCUS       DATBRK010000409         4331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1967857, whole genome shotgun sequence.
ACCESSION   DATBRK010000409 DATBRK010000000
VERSION     DATBRK010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967857"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VIH70_12090"
     CDS             <1..322
                     /locus_tag="VIH70_12090"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY8117148.1"
                     /translation="IVSLEAIFLSIFVLMTQSRESRIGELREELTLQVNLRMEEEITK
                     TLHLVAGLYARLGLKMADDPELRAMLEPLDPKKIENDLAEQISASMPEFQFLKKKKTP
                     AAKV"
     gene            complement(346..3075)
                     /gene="acnA"
                     /locus_tag="VIH70_12095"
     CDS             complement(346..3075)
                     /gene="acnA"
                     /locus_tag="VIH70_12095"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY8117149.1"
                     /translation="MSDPFAARTRLETGDGQVELYRLAEVAELDRLPHTVKILLENLL
                     RRAGSRDVSEDDVRALASWPREAPRADLAFMPSRVLMQDFTGVPAVVDLAAMRSAMER
                     AGGDPSRVSPLVDVDLIIDHSVQVDLFRSPEAYEANIEWEYRRNGERYALLRWAQQAF
                     DGIRVVPPGAGICHQVNLEHLGRVVTVQDGVALPDTLVGTDSHTTMVNGLGVLGWGVG
                     GIEAEAAMLGQPMFLPTPLVVGVRVSGALPAGTTATDLVLTLTQMLREHGVVGRFVEF
                     FGDGCSTLELADRATLSNMCPEYGATSAFWPVDGETLRYLELTGRGDRIDLVERYTKE
                     QGLFRRDGDAEPTFTEIVELDLGAIEPSVAGPKRPQDRVPLSRVWGSFVGSFRDRLEP
                     DPDSNEVGRLLEEGGNPEVGLALDETVEPGTEEPVPLDHGIVRHGSIVIAAITSCTNT
                     SNPTVMLAAGLLAKKAVEAGLDTKPWVKTSLAPGSRVVTEYLDAAGLTPYLDKLGFSL
                     VGFGCTTCIGNSGPLPDDVANAVTENDLAVVAVLSGNRNFEGRIHPLVRASYLASPPL
                     VVAYALAGHVDIDLSSDPLGTSTDGRVVSLRDLWPSAEEVRAAIAASVSSEQFDREYG
                     RIFDGDDRWRALPTPEGPVYAWDPNSTYVREPPFFADLDPAPAEPSDLAGARCLVKVG
                     DSITTDHISPAGSIKPDSPAGRYLLDHGVEQRDFNSYGARRGNHEVMMRGTFANIRLR
                     NELAPGTEGGWTQRSPGDKALSIYDAAMEYQKAGTQLVIIAGKEYGTGSSRDWAAKGT
                     LLLGVRAVIAESFERIHRSNLVGMGVLPLEFVPGETRQTVPLTGFETFSIEGMTEDMT
                     PRAKLTVRARAADGSEKTFKVVSRIDTPEEMSYYRHGGILPYVLRQLVGRERQ"
     gene            complement(3088..3588)
                     /locus_tag="VIH70_12100"
     CDS             complement(3088..3588)
                     /locus_tag="VIH70_12100"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY8117150.1"
                     /translation="MGHLEPRRVASSQVTLVQLMEITDANIAGLVHGGVVMKLVDTAA
                     GLAAFKHAGGMCVTVSMDEMSFLAPVHVGDTVTVHASVNDVGTTSLEIGVRVESEELE
                     TGTRTHTSSAYLVFVALDDEGRPRPVPPLIAETEEEQRRQREAKVRRETRIGHREAIA
                     ELRRGV"
     gene            3629..>4331
                     /locus_tag="VIH70_12105"
     CDS             3629..>4331
                     /locus_tag="VIH70_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117151.1"
                     /translation="MRRILPLVAILAIAAAACSGADEPVGPPSATTAPMTAPATTQAT
                     PSPTSTTEPASPSSGPAEDPDVRLPEGMPAIVDDADDLALIASGDLTPLLPPGASPAT
                     RLSLRDPIDQIVLTWSDGGPLRRRNGLIVWQRSEDGSWYAVYAFTDPVRLGVFGIRVY
                     AGDVTDDAIADVLSFEDVGGSGACGTYRVIASTVGDASEIFRRDACDTQIAISGGDLR
                     IREAVFHAGDPHCCPS"
BASE COUNT          670 a         1544 c         1446 g          671 t
ORIGIN      
        1 gatcgtctcg ctcgaggcga tcttcctgtc gattttcgtg ctgatgacac agagccgcga
       61 gtcacgcatc ggcgagctgc gtgaggagct gacgctgcag gtcaatctcc gcatggagga
      121 ggagatcacc aaaaccctcc acctcgtcgc cggtctctat gcgcggctcg gtttgaagat
      181 ggctgacgat cccgagctgc gagcgatgct ggagccgctc gatccgaaga agatcgagaa
      241 cgatctcgcc gagcagatca gcgcgtcgat gcccgagttt cagttcctca agaagaaaaa
      301 gactccagcg gccaaagtct gaagagagcg cgctgaggcg cgctcctact ggcgttcccg
      361 acctaccagc tggcgcagga cgtacggcag aattccaccg tgccggtagt agctcatctc
      421 ctccggcgtg tcgatgcgcg aaaccacctt gaacgtcttc tcgcttccgt ccgcggcgcg
      481 cgcgcgcacg gtgagttttg cgcgtggggt catgtcctcc gtcattccct cgatcgagaa
      541 cgtctcgaag ccagtgaggg gaacggtctg gcgcgtctca cccgggacga actcgagcgg
      601 cagcaccccc atcccgacca ggttcgagcg gtgaatcctc tcgaacgact cggcgatcac
      661 cgcacgaaca cccagcagca gcgttccctt cgccgcccag tcgcgtgacg aacccgttcc
      721 atattccttg ccggcaatga tgacgagctg agtgccggcc ttctggtact ccatcgccgc
      781 gtcgtaaatc gacagcgcct tgtcgccagg tgagcgctgg gtccagccgc cctcggtccc
      841 cggcgcgagc tcgttgcgca ggcgaatgtt ggcgaacgta ccgcgcatca tcacctcgtg
      901 attcccgcgc cgcgcgccgt aggagttgaa gtcgcgctgc tcgacgccgt ggtcgagcag
      961 gtaccgtccc gcgggcgaat ccggtttgat cgagcctgcc ggcgagatgt gatcggtcgt
     1021 gatggagtca cccaccttga cgaggcatcg cgcgccggcg aggtccgagg gttcggcagg
     1081 tgcggggtcg aggtctgcga agaacggcgg ctcccgcacg taggtcgagt tcggatccca
     1141 cgcgtacacg ggaccctcgg gcgtggggag cgcgcgccac cggtcgtcgc cgtcgaagat
     1201 gcgcccgtac tcgcgatcga actgttccga cgagaccgac gccgcgatcg cagcccgcac
     1261 ctcctcggcc gacggccaca ggtcgcggag cgagaccacc ctgccgtccg tcgaggtccc
     1321 cagggggtcg ctcgagaggt cgatgtcgac gtgtcccgcg agcgcgtagg cgaccacgag
     1381 cggcggcgag gcgaggtagc tcgctcggac cagcggatgg atccgcccct cgaagttgcg
     1441 gttccccgag agcaccgcga ccacggcgag gtcgttctcc gtcaccgcgt tcgccacgtc
     1501 gtcgggcaac ggtccggagt tcccgatgca ggtcgtgcac ccgaacccca cgagcgagaa
     1561 gccgagcttg tccagatacg gcgtcagccc ggctgcgtcg aggtattcgg tcaccacgcg
     1621 cgaacccggg gcgagcgacg tcttgaccca cggcttggtg tccagccccg cctcgacggc
     1681 cttcttcgcg agcaggccgg cggcgagcat cacggtgggg ttcgacgtgt tcgtgcagct
     1741 cgtgatcgcg gcgatcacga tcgaaccatg gcggacgatg ccgtggtcga gcgggaccgg
     1801 ctcctcggtc cccggttcca cggtctcgtc gagagcgagc ccgacctccg ggttgccgcc
     1861 ctcctcgagg agccgaccga cctcgttcga gtcggggtcg ggttcgagcc gatcgcggaa
     1921 cgaccccacg aacgagcccc agacgcgcga gagcgggacg cggtcctgcg gtcgtttcgg
     1981 tccggccacc gagggttcga tcgcgccgag gtcgagctcg acgatctcgg tgaaggtggg
     2041 ctccgcatcc ccgtcacgcc ggaacaggcc ctgctccttc gtgtagcgct cgacgaggtc
     2101 gatccggtcg ccgcgacccg tgagctccag gtagcgcagc gtctcgccgt cgacgggcca
     2161 gaacgcggag gtggcaccgt actccgggca catgttcgac agcgtggcgc gatccgcgag
     2221 ctcgagggtc gagcagccgt caccgaagaa ctcgacgaac cgtccgacca cgccgtgctc
     2281 gcggagcatc tgggtgagcg tgaggaccag gtcggtcgcc gtggtgccgg ccgggagagc
     2341 gcccgagacc cggaccccca cgacgagcgg cgtcggcagg aacatcggct ggccgagcat
     2401 cgccgcctct gcctcgatcc cgcccacgcc ccacccgagg acccccagcc cgttcaccat
     2461 cgtcgtgtgg gaatccgtcc cgaccagcgt gtccgggagc gccacgccgt cctgcaccgt
     2521 gaccacccgc ccgaggtgct cgaggttcac ctggtggcag atccccgcac cgggcgggac
     2581 cacgcgaatg ccgtcgaacg cctgctgcgc ccatcgcagc aacgcgtacc gttccccgtt
     2641 gcgccggtac tcccactcga tgttcgcctc gtacgcctcc ggcgagcgga acaggtcgac
     2701 ctgcaccgag tgatcgatga tcagatccac gtccaccagc ggggagaccc tggaggggtc
     2761 gccgccggcg cgctccatcg ccgagcgcat cgccgccagg tcgacgaccg cgggcacgcc
     2821 cgtgaagtcc tgcatcagca cccgggaggg catgaaggca aggtcggcgc gcggggcctc
     2881 ccgcggccac gacgcgagcg cccggacgtc atcctcggag acgtcgcggc tgccggcgcg
     2941 gcggagcagg ttctcgagca ggatcttgac ggtgtggggg agccggtcga gctcggcgac
     3001 ctccgcgagc cggtagagct cgacctggcc gtcgccggtc tccagtcggg tccgggcagc
     3061 gaacgggtcg ctcacggttc catgctctca gacgccccgg cgaagctcgg cgatcgcctc
     3121 acggtgcccg atcctggtct cgcgccggac cttggcctcg cgctgacggc gttgctcctc
     3181 ctcggtctcg gcgatcagcg gcgggaccgg cctcggcctg ccctcgtcgt cgagcgcgac
     3241 gaacacgagg tacgcgctgg aggtatgggt cctcgtgccc gtctcgagct cctcggattc
     3301 gacgcggacg ccgatctcga gcgaggtcgt ccccacgtcg ttgaccgacg cgtgcacggt
     3361 cacggtgtcc cccacgtgaa ccggcgcgag gaacgacatc tcgtccatcg agaccgtcac
     3421 gcacatgccg ccggcgtgtt tgaacgcggc gaggcccgcg gccgtgtcga cgagcttcat
     3481 cacgacgccc ccgtgtacaa gcccggcgat gttggcgtcg gtgatctcca tcagctggac
     3541 gagcgtcacc tggctcgagg ccacgcgtcg gggttcgaga tgccccacgt gcggattcta
     3601 ggcggcgacg cgccggtagc atgcgcgcgt gcgtcgcatc ctccccctcg tcgcgatcct
     3661 cgccatcgcc gcggcggcct gttccggtgc ggatgaaccg gtcggaccac cgtccgcgac
     3721 gaccgctccg atgaccgctc ccgcgaccac gcaggccacg ccgtcaccca cttccacgac
     3781 cgaacccgcg agcccgagct ccggtcccgc cgaggatccc gacgtccggc tccccgaagg
     3841 catgccggcg atcgtggacg acgccgacga cctcgcgctg atcgcgtcgg gggacctcac
     3901 cccgctcctg cctcccggag cgagccccgc gacccgcctc tcgcttcgag acccgatcga
     3961 ccagatcgtc ctgacctggt cggacggcgg ccctctccgc aggcggaacg ggctcatcgt
     4021 gtggcagcga tccgaggacg ggagctggta cgccgtgtac gccttcaccg atccggtgcg
     4081 cctcggtgtg ttcgggatcc gcgtctacgc cggcgacgtg acggacgacg cgatcgccga
     4141 cgtcctgagc ttcgaggacg tcgggggcag cggtgcctgc gggacgtacc gggtgatcgc
     4201 ctccaccgta ggcgacgcga gcgagatctt ccgccgcgac gcgtgcgaca cccagatcgc
     4261 gatctcgggc ggtgacctcc ggatccgcga ggccgtcttc cacgccggcg acccgcattg
     4321 ctgcccgagc g
//
LOCUS       DATBRK010000410        16909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1968970, whole genome shotgun sequence.
ACCESSION   DATBRK010000410 DATBRK010000000
VERSION     DATBRK010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16909
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968970"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VIH70_12110"
     CDS             <1..665
                     /locus_tag="VIH70_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HEY8117152.1"
                     /translation="RVAFIPRHGVKHQFPPHTINYRANLWAMKELGVQRIVGPCAAGS
                     LQMGVKIGDFVVCDQIVDRTRSRPHTFYDGPVTTHISFADPYCATMRAIIVGKTRELG
                     IGVHDGGTMVVIEGPRFSTRAESAWFNSEGWQTINMTGYPEGLLARELEICYANVSLI
                     TDYDVGVPGVPPVTHEEVVRVFGQNNAHLRELLFSVIPALPTERDCECATALANARFE
                     VE"
     gene            667..843
                     /locus_tag="VIH70_12115"
     CDS             667..843
                     /locus_tag="VIH70_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117153.1"
                     /translation="MSEKVLVTIELRFTTDDDPEALGERIREATSSIVGRQALEDFRV
                     RALPLTPPKGPRPR"
     gene            941..1591
                     /gene="cpaB"
                     /locus_tag="VIH70_12120"
     CDS             941..1591
                     /gene="cpaB"
                     /locus_tag="VIH70_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY8117154.1"
                     /translation="MFLRRWSLRSKVMALLAVVAGAGSFTLVRGYAAELEALRPVPGS
                     DVPVVVADRDLERGAVIDEGSVRLERMPPEYAPPGALRSIAGAVGRTLGSDLAEGEAV
                     TRTRVGGPGGPVASLVASGLRAFVVGSGLPSGVLEPGDRVDVIATFGGPHPYTDTVGS
                     GLQILSIVEQDAGTFQAGGATGPSLVLLVSPETAEQLARATAFGQLTVTIAPVDAV"
     gene            1683..2504
                     /locus_tag="VIH70_12125"
     CDS             1683..2504
                     /locus_tag="VIH70_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY8117155.1"
                     /translation="MLGITQGVTEFAPVSSSGHLIIVPWLFDWPILTDPSLNKTFDVA
                     LHLGTLVGALVYFRHDVARFAAAWFGSLSARRIETTDERLAWALVVGTVPAVIVGATL
                     ESFIEDELGQPWLIAVMLVVFGVVLYGVDRIASSRRHLDDLGVRDGLVLGVAQALALQ
                     PGVSRSGVTMTAARVLTFDRETAARFSFLLSLPVIAGAVLYKGLDLAADGIRPGMGGP
                     FVAGIAASAVSGFLVIAFLLRYLRFHDFALFLWIRVAVAALIFVVIATGTRPATV"
     gene            2736..4247
                     /locus_tag="VIH70_12130"
     CDS             2736..4247
                     /locus_tag="VIH70_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8117156.1"
                     /translation="MGLVVGGVALGTAQLVAGIVNPGAFPILTVGQSVIDATPEWLKG
                     AAIRAFGPSDKSVLLAGIGVVLALAAIALGIASLYRLWVGVVGLAAFGAIGVAAALAR
                     PVAMPRDVLPAIAATAVGLPTLVAIRSCVRFDATGRRPGEATASLPGVIDRRRFLLIA
                     TAGFVGALAAGGAGSFFARRFRADASRAAVGMPTPASRAAAIDGAELHVPGLAPFITP
                     NERFYRVDTALFAPAVTAEDWSLRIHGMVRREITLDYEQLLSRALIERDITLTCVSNV
                     VGGPYIGNARWVGAPLRELLTEAGPLPGADQIVSRSVDGFTIGTPTAVAMDGRDAMLA
                     VAMNGEPLPIVHGFPVRMIVPGLYGYVSATKWIVDIELTRFDAYDPYWVQRGWAEQAP
                     IKTMSRIDTPSPNRQLPAGEIPIAGVAWAQDVGIDRVDVAVDDGPWLMAELAAEDTID
                     TWRQWLYRWEATSGEHLLRVRAIDRTGAPQTPVRTDPFPDGATGHHTISVVVR"
     gene            complement(4263..5597)
                     /locus_tag="VIH70_12135"
     CDS             complement(4263..5597)
                     /locus_tag="VIH70_12135"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY8117157.1"
                     /translation="MPDLLDLCAAAVEAAEGGEHVEAFGEERSTSSVEAHRGEIEGLT
                     FAESRGVGVRVISDGRLGFAHAADPSVDEVREAVSRARENAAVASPDPFNALPAAEPI
                     EPLEGLFSDALAGMTTDQKVPIALGMERAATTMDPRVRKTESVRYGDAVGAAAIASTG
                     GVRVEYRATGCWVVVVTLAEQDGDTQTGFSYKIARTVEDLDHERVVAEAVERAARMLG
                     ATKPTTARVPVLLDPVAASSFLGVLSDGLSAESVQKQRSLFAPLLGERVAADVLTLVD
                     DGRLLDGPGAAPFDAEGVPTRRTELLSAGVLEGFLHNTYTALRGDTRSTGNAVRGYRS
                     SPGVGTTNLFVEPGDRSLEQLLREAEGGVLVQDVTGVHSGANPISGEFSVGATGLRIS
                     GGALGEPMREMTIASTIPEMLRSVIALGSELRWFSSIGVPHVLLGEMTLAGV"
     gene            complement(5590..6975)
                     /locus_tag="VIH70_12140"
     CDS             complement(5590..6975)
                     /locus_tag="VIH70_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY8117158.1"
                     /translation="MLDRDLAAEVLRTARARGGGFAELYVEERLGISVRLDDGKVEEV
                     TTGLDRGAGVRVGHGASFGYAYSNRLDRDALLHAAGAAAASVNEGGPGSVVDLRTLEP
                     PVTHAALVDAASVPTERKVGWVREADEAARALGAEIHQVTATYGDSIQRLLIATSDGR
                     WVEEVRPRVRLTVSAVAGRDGTIQTGFHGPAGLAGAEFIEANPPWATAEVAARRAITM
                     LDAEPAPAGETSVVLAPGMGGVLFHEAVGHPLEADIVDKEASVYRGRIGERLASEILS
                     GVDDATVPNAWGSFSFDDEGTPAQRTVLFEGGELRGWLYDRLRAEKDGVPSSGNGRRQ
                     SYAHPPIPRMTNTYILSGSSTPDRILSDTERGVFVTALAGGQTNPATGDFVFGCAEAY
                     LIERGQATKPIRGANLIGRAIEVMSNVDAIAADFDIWPGMCGKDGQGVPVGSGSPTLR
                     ISRMTVGGTGA"
     gene            7241..8929
                     /locus_tag="VIH70_12145"
     CDS             7241..8929
                     /locus_tag="VIH70_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117159.1"
                     /translation="MGPDAPVYLWWSRLAGAEGLSAIAHRPGAPALSLVLEGTLAVSV
                     VQATAALEVVLGVAVGLASAALVRRRAGAVGSALAGLLAGTFAVHLAAGYLANLVMAA
                     AFLAAGALLDDPRRRAAVLAAVVLAGGGLAHPPFLLLCVAILLVAAAVAWRGDRREAL
                     RIAGAAAGGAAALGIGLLAVRPGTPPLEVDTSKDAFLRRAGLTSELRAAYLDRFVHRW
                     TRYVEWVSVPLAAIGFTAPSGNAGRILRSWFVLTFVGAGFALATGWLPADRFVTFGFA
                     IPILAALGLVRLWRRLERRRALAVVVTAALTLAMLAGSAIAWNRQDPFISEEEVRALV
                     LANKAVSRLEPGTPLAFLVNEPDDTVSFLATRAGNVIRAGVPPDRIRDVVVVVPPLDA
                     RTGSGERGALERLTARDLAAAEDREGRSAAVIVLTPFDEVDRPADAIMIGSALVTGDG
                     FPLADPLEPASAARIGWAAIATLALLGLAGYGWARIGLADTITAAAAAPAIGAAVLIL
                     VAAALDAVGVPLGRVAGALAASALAGGGGYLIRFVLERRAGSGAAPQVEEQPAE"
     gene            complement(8828..10351)
                     /locus_tag="VIH70_12150"
     CDS             complement(8828..10351)
                     /locus_tag="VIH70_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117160.1"
                     /translation="MERRRTGVDRTASRVALAASVASFVFVALVAATPGSPFTPILPS
                     EPAGPFRWLAELVGLDGVHGSALAAVGVVAVASAAAAFVLVLRECRRGTISARTAIGL
                     AVAYHVALLFLPLLFSRDVYSYAYYGRIAAAYHANPYVATPADFPHDALSSFVGPKWG
                     DTPAVYGALWTQVSALVTRAAGDVGAMISAFRVIAIAASLGTVFVVAGLVRRVRPERE
                     AFAVALVGLNPVVLFQSAASGHNDLLVALAVAAGLALVFSGRELWATAALAIGTLVKV
                     TAAVPLLVWWVAVAARRPRGERLRTLAPHLGLAAAFALVAAVPFANTEDPTLGAVELG
                     SHEGWLAPSRFFRRVVDAVSGDVLGVVPRIAFAVIGVAAVVVVATAIWRRRPEIGSAL
                     VGASWGWGLLCLMLLGPVLLPWYVTWALPLGWLLPRVPRTVLIGTGLALTVSQWTSEP
                     AGFGSAYDANLLVGHYVITPVVIGLLGWLLLDLWRRTRSGAPLEDEPDQVPAAAGQR"
     gene            complement(10388..11335)
                     /locus_tag="VIH70_12155"
     CDS             complement(10388..11335)
                     /locus_tag="VIH70_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8117161.1"
                     /translation="MRRSLVALVAVAIALPACAGDGAGGAMPSDPANAATTVAPATGP
                     SGTVSPSPVADAPTAAIPEPDAWIPGRADPLAVRLEATWEARREAVRRWVRTGEPDAW
                     PPPEDLELLVLYEQRIYRVLSAHDRLAVAVLDRLDGPLAAEARSNVRAGGALYDHFSP
                     LKTLPDFRTRAPEPADLLLRYFEKGERRFGVDWELLAAVMLIETRMGRIASSSSAGAQ
                     GPMQFLPSTWEVYGLGGDIRDEHDAVLGAANYLSAFGSPGNDRGALSHYNPVAAYVTA
                     VIGYANAMSRDPDLFYAYYNWQVFVRTRHGDVRLTGPGL"
     gene            complement(11332..12930)
                     /locus_tag="VIH70_12160"
     CDS             complement(11332..12930)
                     /locus_tag="VIH70_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8117162.1"
                     /translation="MGFIELADVAYALPGGWTLFEGVSFRVPEGEHAGLVGANGIGKS
                     TLLRLIVGDDRPTAGSIQTDGRVGLMRQFIGSQVRPTTVRDFLLAYAEPAVSDAAIRV
                     AKAERWMHDHPGEQAQMRYADALANWGEAGGYEAEVLFDQCTHAAFGYGYPECAERKI
                     ETLSGGERKRLALEVILRSRFDTVMLDEPDNALDIQGKRWLEDAIAASPKTVLFVSHD
                     RTVLDRAATRIVTLEGRAAWTHHGGFSGYAAARDARVDRLDQDHRRYQAEHRRLSDMV
                     KEMRRKSAYNDKWASKARSAQHRLDRFEDREKPPEKAEVQNVRMRIEGGRTGKVALRV
                     TGLSIAGIVGPFDAEVWFGERIGVIGPNGTGKTHFMRLLAGEEVSHEGGWKLGARVEP
                     ALFAQLHERGDVADLPIVEVLRKRGMTMTESMAALRRYEIDQVGRNPFQLLSGGQQAR
                     FQLLLMEVDSPTMLLLDEPTDNLDVASADALQDAVDRYQGTVVAVTHDRWFMRLMDRF
                     LFFDGDGSVRGLLESPYEDVAAVG"
     gene            complement(12969..14015)
                     /gene="rlmN"
                     /locus_tag="VIH70_12165"
     CDS             complement(12969..14015)
                     /gene="rlmN"
                     /locus_tag="VIH70_12165"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY8117163.1"
                     /translation="MTTAYDLTLDQLTERLGDWGEPPFRARQVHRQLWQRAATYDRMA
                     DVPGRLRERLEAELPIGVEVLEERQADRGATRKALLRIGGRHVIETVLMGYRDRVTVC
                     LSTQTGCAMGCGFCATGQMGLRNNLTAGEIAAQAVWARREAAALPASTPRRLSNAVFM
                     GMGEPLSNERHTFGALGLLTGEMGMGDRHVTVSTVGVAPAIRRLAEAYPQIGLAVSLH
                     AADDRLRNRLVPVNELWPLEELVDAIEAWRRVTRRRPSIEWAMIDRTNDADEQAERLA
                     PIALRLRAHVNLIPLNPTPGWPSKPSPASRIRAFASILDDAGVAVTIRDTRGREIDAA
                     CGQLSLEHERAAAR"
     gene            complement(14043..14573)
                     /locus_tag="VIH70_12170"
     CDS             complement(14043..14573)
                     /locus_tag="VIH70_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117164.1"
                     /translation="MLATSIYMVLFRVIHVMLGIAWGGALFLLVVFLQPTAKAIGPAA
                     GPFMRELLGVRHLTDWLLRIAGATIVAGGFLFWHDIQVYGGLGDFLGSAFGLWLTIGA
                     LAAIVAVAIGGALTKPALERSLAVGAQIAQAGDQPPPGLVQELAALQARGRSLAILNL
                     ALVSVAAFAMSTARYW"
     gene            14653..15564
                     /gene="mmuM"
                     /locus_tag="VIH70_12175"
     CDS             14653..15564
                     /gene="mmuM"
                     /locus_tag="VIH70_12175"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEY8117165.1"
                     /translation="MTADPFARVRETHGTLVLDGGLATELVRRGADLRDPLWSAKVLV
                     EDPDAIRRVHEAYFEAGADVAISASYQATFEGLAGRGIERTQAATLMRRSVELARDAA
                     SGDRFVAASVGPYGAMLGNGAEYTGDYDKDEDELVRFHVPRLEALAEAGPDAFAVETI
                     PSFVEAGALARALERVPEIPAWVSFSCRDGGHVCDGTPIASAVEIVAPAPSVIAVGVN
                     CTSPLHVERLVETISATTDKPVVCYPNRGSFWDRMRKTWTDPPRQDARPPLRPLAWRE
                     AGASLIGGCCGTTPEDIAAIAAALGRA"
     gene            complement(15572..16147)
                     /locus_tag="VIH70_12180"
     CDS             complement(15572..16147)
                     /locus_tag="VIH70_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8117166.1"
                     /translation="MTAEVHILSEGYLGDGGDRVGSTVGFARDGGVLVVIDPGLVPGR
                     EAILRPLAELELAPDQITDVVLSHHHPDHTLNTALFPDARVHDHWAWYRDDLWVDRPA
                     EGFELSSSIRLIETPGHSPQDVSTLVDTDDGLVVFTHLWWTATVPTEDPFAPDPSVLH
                     ENRARVLALRRLRTIVPGHGAPFEPGPDTPL"
     gene            complement(16270..>16909)
                     /locus_tag="VIH70_12185"
     CDS             complement(16270..>16909)
                     /locus_tag="VIH70_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8117167.1"
                     /translation="AVREARDRGMPAFAETCPQYLYLSLDDLGRPGFEGAKFVCSPPL
                     RPADHQEELWKGLRGDDLQIVSTDHAPFNYVGQKDLGKDDFSKIPNGLPSVEDRFTLL
                     YGGVEAGHIGLNRFVEIVATAPAKMFGLYPRKGTIAPGSDADVVVFDPRVEREISAKT
                     HHMNVDYSAYEGRKVRGLPEVVLQRGQVLVQDGTFHGKPGQGRFLPRSRISV"
BASE COUNT         2319 a         6218 c         6010 g         2362 t
ORIGIN      
        1 atcgggtcgc cttcatcccg cggcacggcg tcaagcacca gttcccaccg cacacgatca
       61 actaccgcgc caacctgtgg gccatgaagg agctcggcgt gcagcggatc gtcgggccgt
      121 gcgcggcggg atcgctgcag atgggcgtga agatcggcga cttcgtcgtg tgcgaccaga
      181 tcgtcgatcg gacgcgttcg cgcccgcaca ccttctacga cggccccgtg accacgcaca
      241 tctcgttcgc cgacccgtac tgcgcgacga tgcgggcgat catcgtgggc aagacgcgcg
      301 agctcggcat cggcgtgcac gacggcggga cgatggtcgt gatcgagggt ccccggttct
      361 cgacgcgggc cgagtccgcc tggttcaact cggagggctg gcagacgatc aacatgaccg
      421 ggtaccccga gggcctgctc gcccgcgagc tcgagatctg ctacgccaac gtctcgctga
      481 tcaccgacta cgacgtcggc gtgcccggcg tgccgcccgt tacccacgag gaggtcgtcc
      541 gggtgttcgg acagaacaac gctcacctgc gcgagctgct gttcagcgtc atcccggcgc
      601 tgcccaccga acgcgactgc gagtgcgcga cggcgctcgc gaacgcacgg ttcgaggtgg
      661 agtgacatga gcgagaaggt cctggtgacg atcgagctgc ggttcacgac cgacgacgac
      721 cccgaggcgc tcggcgagcg catccgcgag gcgacctcgt cgatcgtggg gaggcaggcg
      781 ctggaggact tccgcgtccg cgcgctgccg ctcaccccgc cgaagggacc tcgcccacgc
      841 tgacgcaatg gtctgacgcg acgggtgtga cccggttcac ggcgcgacac ggctccatcg
      901 cgtagcgtct cgaaccccgc tctcggacag gaggcccggc gtgttcctgc gacggtggtc
      961 gctgcgctcc aaggtgatgg cgctcctcgc cgtcgtggct ggcgccggct cgttcacgct
     1021 cgtccgcggc tacgcggcgg agctcgaggc gctccgcccg gttcccggga gcgacgttcc
     1081 ggtcgtggtc gcggaccgag acctcgagcg cggggccgtg atcgacgagg gatcggtccg
     1141 cctggagcgg atgccgcccg aatacgcacc gcccggcgcc ctccgctcga tcgcgggcgc
     1201 ggtggggcgg acgctcggct ccgatctcgc cgagggcgag gccgtcacca gaacgcgggt
     1261 cggcggcccg ggcgggccgg tggcctcgct cgtcgcgagc ggcctccgcg cgttcgtggt
     1321 gggttcgggt ctgccgtccg gcgtgctcga gcccggcgac cgcgtcgacg tgatcgcgac
     1381 gttcggcgga ccgcacccgt acaccgacac ggtcggcagc gggctccaga tcctgtcgat
     1441 cgtcgagcag gacgcgggga ccttccaggc cggcggcgcc accggcccgt ccctcgtcct
     1501 gctcgtcagt ccggagaccg ccgaacaact cgcgcgcgcg acggcgttcg gacagctcac
     1561 cgtcacgatc gcgccggtcg acgccgtctg aggatcggcc gagatcgcgg ctcggcgcgg
     1621 ctaggattgc cgcgtcctcc gcgacgtcga ggagaccacc cctgaccatc ctgcaggcga
     1681 tcgtgctcgg catcacgcag ggcgtgaccg agttcgcgcc ggtctccagc tcgggacacc
     1741 tgatcatcgt gccgtggctc ttcgactggc cgatcctcac cgatccgagc ctgaacaaga
     1801 ccttcgacgt cgcgctgcac ctcgggacgc tcgtcggcgc gctcgtctac ttccgacacg
     1861 acgtcgctcg cttcgcggcc gcgtggtttg gctcgctctc ggcccgcagg atcgagacga
     1921 ccgacgaacg cctggcctgg gcgctcgtcg tcgggacggt cccggccgtc atcgtcgggg
     1981 caacgctcga gagcttcatc gaggacgagc tcgggcagcc gtggctgatc gccgtgatgc
     2041 tcgtggtgtt cggtgtcgtg ctgtatgggg tggaccggat cgcgtcatcc cgcaggcacc
     2101 tggacgatct cggcgtccgt gacgggctcg tgctcggcgt cgcccaggcc ctcgcgctcc
     2161 agccgggcgt gtcgcgttcg ggggtcacga tgacggcggc ccgcgtgctc acgttcgacc
     2221 gcgagaccgc cgcccggttc tcgttcctgc tctcgctgcc ggtgatcgcg ggggcggtgc
     2281 tctacaaggg tcttgacctg gccgccgacg ggatccggcc cggcatgggt gggccgttcg
     2341 tcgcggggat cgccgcctcc gccgtgagtg gcttcctggt gatcgctttc ctgctccgct
     2401 atctgcgctt ccacgacttc gcgttgttcc tgtggatccg ggtagcggtc gcggcgctga
     2461 tcttcgtggt gatcgcgacc gggacgcgtc cggcgaccgt ctgagctcgg actcgcgttc
     2521 ggaagaacgg tgtggtgagc gggaggccac ggaagcgaac cgttgttcat cggcgcgtaa
     2581 ggaatcgatc tgctctcgtg cctcatgctg agtgaatgac cgccacggat ccgtcatcag
     2641 acacgcctcg tgggcccgat ccgaccactg gagatcggcc ccccgatccc tccgccttgc
     2701 gacggcgacg ccgggcggcg ggcatcggct cgctggtggg attggtcgtg ggcggggtcg
     2761 ccttggggac ggcccagctg gtcgccggca tcgtgaaccc gggagcgttc ccgatcctga
     2821 ccgtcggcca gagcgtgatc gacgcgactc cggagtggct gaagggcgcc gcgatccgtg
     2881 cgttcggacc gagcgacaag tccgtcctgc tcgcagggat cggcgtcgtc ctcgccctcg
     2941 ccgcgatcgc cctcgggatc gcatccctgt accgcctctg ggtgggggtg gtcgggctgg
     3001 cggcgttcgg cgcgatcggg gtggccgcgg cgctcgcgag gcccgtggcc atgccaagag
     3061 acgtcctgcc cgcgatcgcc gcgacggccg tgggtctgcc caccctcgtg gcgatccgaa
     3121 gctgcgtgag gttcgatgcg acggggcgac gacccgggga ggcgacggcg agcctccccg
     3181 gcgtgatcga tcgtcggcgg ttccttctga tcgccacggc cggcttcgtc ggtgcactcg
     3241 ccgccggcgg ggcggggagc ttcttcgcgc gccgcttccg ggcagacgca tcgagggctg
     3301 cggtcggcat gcccacgccg gcgagtcgcg cggcggcgat cgacggcgcc gagcttcacg
     3361 tgccgggact ggcgccgttc atcaccccca acgagcgctt ctatcgggtc gatacggccc
     3421 tgttcgctcc cgcggtgacg gccgaggact ggtccctccg catccacggg atggtgcggc
     3481 gggagatcac cctcgactac gaacagctct tgtcgcgtgc cctcatcgag cgagacatca
     3541 cgctgacctg tgtctcgaac gtcgtcgggg gtccctacat cggcaacgcg cgctgggtcg
     3601 gggcacccct tcgagagctg ctcaccgagg cgggaccgtt gcccggcgcg gaccagatcg
     3661 tgagccggtc ggtggacggg ttcacgatcg gcacgccgac ggcggtcgcg atggacggtc
     3721 gggacgcgat gctcgccgtc gcgatgaacg gcgagccgct gcccatcgtc cacggtttcc
     3781 cggtccggat gatcgtgccc ggtctgtacg ggtacgtctc cgcgaccaag tggatcgtcg
     3841 acatcgagct cacgaggttc gacgcctacg acccgtactg ggtgcagcgc ggctgggcag
     3901 agcaggcgcc gatcaagacg atgtcgagga tcgacacgcc gtcgccgaac cggcaactgc
     3961 ccgccgggga gatcccgatc gccggagtcg catgggcgca agacgtgggg atcgaccgcg
     4021 tcgacgtggc cgtcgacgac ggtccgtggc tgatggcgga gctagccgcc gaggacacca
     4081 tcgatacctg gcgccagtgg ttgtaccgat gggaggcgac gtcgggtgag cacctcctgc
     4141 gggttcgggc gatcgaccgg accggcgccc cccagacacc cgtccgtacc gacccgttcc
     4201 cggacggggc caccggacac cacacgatct ccgtggtcgt ccgatgaagg caccagggcg
     4261 gatcacacgc cggcgagcgt catctcgccg aggagcacgt gcggcacccc gatcgaggag
     4321 aaccatcgga gctcgctccc gagcgcgatc acgctccgga gcatctcggg gatcgtgctc
     4381 gcgatcgtca tctcgcgcat cggctcgccg agcgcgccac ccgagatgcg cagacccgtc
     4441 gccccgaccg agaactcgcc gctgatcggg ttcgcgcccg agtgcacgcc ggtcacgtcc
     4501 tgcacgagca cgccgccctc cgcctcgcgc aggagctgct cgagcgaccg gtcgccgggc
     4561 tcgacgaaca ggttggttgt gccgacgccc ggggacgagc ggtagccgcg caccgcgttg
     4621 ccggtcgaac gcgtgtcgcc gcgcaacgcc gtgtacgtgt tgtggaggaa cccttcgagc
     4681 acgccggccg acaggagctc ggtccggcgg gtcggcaccc cctcggcgtc gaacggcgcc
     4741 gcacccggcc cgtcgagcaa ccgcccgtcg tcgacgagcg tgaggacgtc ggccgcgacg
     4801 cgctcgccga gcagcggggc gaacagcgac cgctgcttct gcacggactc ggccgacaac
     4861 ccgtcagaca gcacgccgag gaacgacgac gcggcgacgg gatcgagcag caccggcact
     4921 cgggcggtcg tcggcttggt cgcgccgagc atccgcgccg cgcgctcgac ggcctccgcg
     4981 accacgcgct cgtggtcgag gtcctccacc gtgcgggcga tcttgtagga gaagccggtc
     5041 tgcgtgtcgc cgtcctgctc ggcgagcgtg accaccacga cccagcagcc ggtggcgcgg
     5101 tactcgaccc gcacgccccc ggtggacgcg atcgcggccg cgcccaccgc atcgccgtac
     5161 cgcaccgact cggtcttgcg gacccgagga tccatcgtgg tcgccgcccg ctccatcccg
     5221 agcgcgatcg gcaccttctg atcggtcgtc atgccggcca gggcatcgga gaacaggccc
     5281 tccagcggtt cgatcggttc ggcggccggc agcgcgttga acggatcggg cgaggcgacc
     5341 gccgcgttct ctcgcgcccg cgagaccgcc tcccgcacct cgtcgacgga tgggtcggcc
     5401 gcgtgcgcga acccgagacg accgtccgag atcactcgga cgcccacacc gcgcgactcg
     5461 gcgaacgtga gcccctcgat ctcgccgcga tgcgcctcca ccgacgacgt cgaacgctcc
     5521 tcgccgaacg cctcgacgtg ctcgccgccc tcggccgcct cgaccgcggc ggcgcacagg
     5581 tccaacaggt caggcaccgg tgcccccgac cgtcatccgg gagatccgca gcgtcggcga
     5641 gccgctcccg acggggacgc cctgcccgtc cttgccgcac atccccggcc agatgtcgaa
     5701 gtcggcggcg atcgcgtcca cgttcgacat cacctcgatc gcccggccga tcaggttcgc
     5761 gccgcggatc ggtttcgtgg cctggccccg ctcgatcaga taggcctccg cgcacccgaa
     5821 cacgaagtcg ccggtggcgg ggttcgtctg cccgcccgcg agcgcggtga cgaagacccc
     5881 gcgctcggtg tcggacagta tccggtcggg cgtggacgat ccgctcagga tgtaggtgtt
     5941 cgtcatgcgc gggatcggtg ggtgggcgta cgactggcgg cgcccgttcc cgctcgaagg
     6001 cacgccgtcc ttctccgccc ggagccggtc gtagagccac ccgcgcagct cgcccccctc
     6061 gaacagcacc gtccgctgcg ccggcgtgcc ctcgtcgtcg aacgagaacg agccccacgc
     6121 gttcggcacc gtggcgtcgt cgaccccgct caggatctcg gaggcgagcc gctccccgat
     6181 ccggccgcgg tacacgctcg cctccttgtc cacgatgtcc gcctcgaggg gatgccccac
     6241 cgcctcgtgg aagagcacgc cgcccatccc gggtgcgagc accaccgagg tctcgcccgc
     6301 aggggccggt tccgcgtcga gcatcgtgat cgcccggcgc gcggccacct cggcggtcgc
     6361 ccacggcggg ttggcctcga tgaactccgc gccggccagg cccgccggac cgtgaaaccc
     6421 ggtctggatc gtgccgtcgc gccccgcgac cgccgacacg gtcagtcgga cgcgtggccg
     6481 gacctcctcg acccaccgtc cgtcgctggt cgcgatcagc aggcgctgga tcgagtcgcc
     6541 gtaggtcgcg gtcacctggt ggatctcggc gccgagcgcg cgagccgcct cgtcggcctc
     6601 gcggacccac ccgaccttgc gttcggtcgg gaccgacgcg gcgtcgacga gcgccgcgtg
     6661 cgtgaccggc ggctcgagcg tgcgcaggtc gacgaccgag cccggcccgc cctcgttcac
     6721 cgacgcggcc gcagccccgg cggcgtgcag cagcgcgtct cggtcgaggc ggttcgagta
     6781 cgcgtacccg aacgacgcgc cgtgccccac ccggacgccg gcgccgcgat cgagcccggt
     6841 ggtgacctcc tcgaccttgc cgtcgtccag gcgcaccgag atgcccaggc gctcctccac
     6901 gtacagctcc gcgaacccgc cgccgcgggc gcgagcggtg cggaggacct ccgcggcgag
     6961 atcgcggtcg agcacggcgc gatgctagca gcggcgggtc gagcgacggc cccgtgatac
     7021 gttccggcga ccgagcccac ggacgtcacg acgggtgacg ccggatcccc tgcggaggat
     7081 tggatgagga acgcgcgacc gaccggttgg ttcgacgcgc aggggtagcg ccacggatcg
     7141 cagggcgaac gggaccgacg cgttcctggt cggggccctc gccctggtcc tgctcgggtg
     7201 gttcctctgg ccgttcgccg cgcgaggcgc cgggttcccg gtgggtcccg acgcgccggt
     7261 ctacctttgg tggtcccggc tcgcgggcgc cgaagggctc tcggcgatcg cccaccggcc
     7321 cggcgcgccg gcgctgtcac tcgtcctgga gggaaccctc gcggtgtcgg tcgtccaggc
     7381 gaccgcggcc ctggaggtcg tcctgggcgt cgcggtcggc ctcgcgtcgg cagccctggt
     7441 gcgccggcgg gcgggcgccg tcggatcggc gctcgcgggg ctgctggcag ggacgttcgc
     7501 cgtccacctc gcggccgggt acctcgccaa cctcgtcatg gcggcggcgt tcctcgcggc
     7561 gggcgcgctg ctggacgacc cccgacgccg ggcggccgtg ctcgccgcgg tcgtgctcgc
     7621 gggcggtggc ctcgcccacc cgccgttcct cctgctgtgc gtcgcgatcc tgttggttgc
     7681 ggcagccgtc gcgtggcgcg gcgatcggcg cgaggcgctc cggatcgcgg gggccgccgc
     7741 cggcggcgcc gcagcgctcg ggatcgggtt gcttgccgtg cggccgggga cgccgccgct
     7801 ggaggtcgac acgtcgaagg acgccttcct gcgtcgtgcg ggcctcacgt ccgagctgcg
     7861 cgccgcgtac ctcgaccgct tcgtccaccg ctggacccgc tacgtggagt gggtctcggt
     7921 gccgctcgcc gcgatcggct tcacggcacc gagcggcaac gcgggccgga tcctgcgctc
     7981 gtggttcgtc ctcaccttcg tcggcgcggg tttcgccctc gcgacggggt ggctccccgc
     8041 cgatcggttc gtgacgttcg ggttcgcgat cccgatcctg gccgccctcg ggctcgtccg
     8101 cctgtggcgc cggctcgaac ggcgtcgagc gctcgccgtc gtggtgaccg cggccctcac
     8161 gctcgcgatg ctcgcgggct cggcgatcgc gtggaaccgc caggacccgt tcatctccga
     8221 ggaggaggtt cgagcgctcg tgctcgcgaa caaggccgtc tcacggctcg agccggggac
     8281 cccgctcgcg ttcctcgtca acgagcccga cgacaccgtg agcttcctcg cgacccgagc
     8341 gggcaacgtc atcagggcgg gcgtgccgcc ggatcggatc cgtgacgtcg tcgtcgtcgt
     8401 gccaccgctc gacgcccgaa cggggagcgg cgaacgcggc gcgctcgaac gcctcacggc
     8461 acgcgacctg gccgccgccg aggatcggga aggccggagc gccgcggtga tcgtgctgac
     8521 cccgttcgac gaggtggacc ggccggcgga cgcgatcatg atcggctccg cgctcgtcac
     8581 cggcgacggg ttcccgctcg ccgatccgct cgaacccgcg tcggccgcgc gcatcgggtg
     8641 ggcggcgatc gcgacgctcg cgctgttggg gttggccggc tacggctggg cccggatcgg
     8701 gctcgcggac acgatcacgg ccgccgcggc cgccccggcg atcggcgcgg cggtgctgat
     8761 cctggtcgcg gccgcgctcg acgcggtcgg cgtcccgctc gggagggtcg ccggggcgct
     8821 cgccgcctca gcgctggccg gcggcggcgg gtacctgatc cggttcgtcc tcgagcggcg
     8881 cgccggatcg ggtgcggcgc cacaggtcga ggagcagcca gccgagtagc ccgatcacga
     8941 ccggcgtgat cacgtagtgc cccaccagga ggttcgcgtc gtacgcggaa ccgaacccgg
     9001 ccggctccga ggtccattgc gacacggtga gcgcgagccc ggtgccgatc agcacggtgc
     9061 gggggacgcg cggcagcagc cagccgagcg gcagcgccca cgtgacgtac cacgggagca
     9121 ggaccggccc gagcagcatc aggcagagca gaccccaccc ccacgacgcc ccgacgagcg
     9181 ccgagccgat ctcgggccgc cgacgccaga tggccgtggc caccacgacg accgccgcca
     9241 ccccgatcac cgcgaacgcg atccgcggca cgaccccgag gacgtcgccc gagaccgcgt
     9301 cgaccacccg ccggaagaac ctggacggcg cgagccagcc ctcgtgtgaa ccgagctcga
     9361 cggcgcccag ggtcggatcc tcggtgttcg cgaacggcac cgcggcgacg agcgcgaacg
     9421 ccgcggccag cccgaggtgc ggcgccaggg ttcggaggcg ttcgccgcgc ggccgccggg
     9481 cggcgaccgc gacccaccac accaggagcg gcaccgcggc cgtgaccttg acgagcgtcc
     9541 cgatcgcgag cgcggcggtc gcccacagct cgcgacccga gaagaccagc gcgagcccgg
     9601 ccgcgaccgc cagcgccacc agcaggtcgt tgtgcccgct cgccgcgctc tggaacagca
     9661 cgaccgggtt cagcccgacc agcgcgaccg cgaacgcctc gcgctcgggc cggaccctgc
     9721 ggaccagacc cgccaccacg aacacggtcc cgaggctggc ggcgatcgcg atcacccgga
     9781 acgccgagat catggcgccg acgtcgccgg cggcgcgggt cacgagcgcc gagacctggg
     9841 tccacagcgc cccgtacacc gccggcgtgt cgccccactt cgggccgacg aagctggaga
     9901 gggcgtcgtg agggaagtcg gccggcgtcg cgacgtacgg gttcgcgtgg tacgcggcgg
     9961 cgatcctgcc gtagtaggcg tacgagtaga cgtcgcgcga gaacaggagc ggcaggaaca
    10021 gcagcgcgac gtggtaggcg accgcgagcc cgatcgccgt tcgcgccgag atcgtcccgc
    10081 ggcggcactc acggaggacg aggacgaacg cggccgccgc cgacgccacc gcgaccacgc
    10141 cgaccgccgc gagcgcgctc ccgtgcacac cgtcgagccc cacgagctcg gccagccagc
    10201 ggaacggacc agcgggctcg gaaggaagga tcggcgtgaa cggcgacccc ggcgttgccg
    10261 cgacgagcgc cacgaacacg aacgacgcga ccgaggccgc gagcgcgacg cgagacgccg
    10321 tccggtcgac gcctgttcgc cgacgttcca tcgggcgagc gtagctcgcc gccggggtcg
    10381 cggcgggcta cagccccgga ccggtgagcc gcacgtcccc gtgcctggtg cgcacgaaca
    10441 cctgccagtt gtagtacgcg tagaacaggt ccgggtcgcg cgacatcgcg ttcgcgtacc
    10501 cgatgaccgc cgtgacgtac gccgccaccg ggttgtagtg ggacagcgcc ccgcggtcgt
    10561 tgcccgggga gccgaaggcg ctcaggtagt tggcggcgcc gaggaccgcg tcgtgctcgt
    10621 ctcggatgtc gccacccagg ccgtagacct cccaggtcga cgggaggaac tgcatcggcc
    10681 cctgcgcgcc ggccgagctg ctcgaggcga tccgacccat ccgcgtctcg atcagcatca
    10741 cggccgcgag cagctcccag tccaccccga accgccgttc gcccttctcg aagtaccgca
    10801 ggaggaggtc ggcgggttcg ggtgcccggg tccggaagtc gggcagggtc ttcaacggcg
    10861 agaagtggtc gtacagggcg ccgcccgccc ggacgttcga cctcgcctcg gcggccaggg
    10921 gaccgtcgag ccggtccagg accgcgaccg ccaggcggtc gtgggccgac aacacgcggt
    10981 agatccgctg ctcgtacagg acgaggagct cgaggtcctc cggcggcggc caggcatccg
    11041 gctcgccggt gcgaacccat cgacggacgg cctcgcgccg ggcctcccac gtggcctcga
    11101 gccggacggc cagcggatcg gcccggccgg ggatccaggc gtcgggctcg gggatcgccg
    11161 cggtcggggc atcggcgacc ggggagggcg acacggttcc gctcggaccc gtcgccggcg
    11221 ccaccgtcgt cgccgcgttc gccggatcgg agggcatcgc acctccggca ccgtcgccgg
    11281 cgcacgccgg cagcgcgatc gccacggcga cgagggcgac gagcgaccgc ctcatccgac
    11341 cgcggccacg tcctcgtacg gcgactcgag cagcccgcgc accgagccgt cgccgtcgaa
    11401 gaacaggaag cggtccatca gccgcatgaa ccagcggtcg tgggtgacgg cgacgaccgt
    11461 cccctgatag cggtcgacgg cgtcctggag cgcgtccgcc gaggccacgt cgaggttgtc
    11521 ggtcggctcg tcgagcagca gcatcgtggg cgagtccacc tccatgagca ggagctggaa
    11581 ccgggcctgc tggccgccgg acaggagctg gaacgggttt cgacccacct ggtcgatctc
    11641 gtagcggcgg agcgccgcca tcgactcggt catcgtcatc ccccgcttgc ggagcacctc
    11701 gacgatcggc agatcggcca catcgccgcg ctcgtgcagc tgcgcgaaca gggcgggctc
    11761 gacccgcgcg ccgagcttcc atccgccctc gtgcgagacc tcctcgcccg ccagcagccg
    11821 catgaagtgc gtctttccgg tcccgttggg accgatcacc ccgatccgct cgccgaacca
    11881 gacctcggcg tcgaacggcc cgacgatccc ggcgatcgac agcccggtca cccggagcgc
    11941 gaccttgccc gtgcgcccgc cctcgatgcg catccggacg ttctgcacct cggccttctc
    12001 gggcggcttc tcgcgatcct cgaaccggtc gagccggtgc tgcgccgacc gggccttcga
    12061 ggcccacttg tcgttgtacg cggacttgcg ccgcatctcc ttgaccatgt ccgagagccg
    12121 ccggtgctcg gcctggtagc gcctgtgatc ctggtcgagc cggtcgaccc gggcgtcgcg
    12181 cgccgcggcg tagcccgaga agccgccgtg gtgcgtccac gcggcgcgcc cctcgagcgt
    12241 gacgatcctg gtcgcggcgc ggtcgagcac ggtccgatcg tggctcacga acaggacggt
    12301 cttggggctg gccgcgatcg cgtcctcgag ccagcgcttg ccctggatgt cgagcgcgtt
    12361 gtcgggctcg tcgagcatca cggtgtcgaa ccgcgaccgc aggatcacct cgagcgcgag
    12421 gcgcttgcgc tcgccgccgg agagcgtctc gatcttgcgt tcggcgcact cggggtagcc
    12481 atatccgaac gcggcgtgcg tgcactggtc gaacagcacc tcggcctcgt acccgccggc
    12541 ctctccccag ttcgcgagcg cgtccgcgta ccgcatctgc gcctgttcgc ccgggtgatc
    12601 gtgcatccag cgctcggcct tggcgacacg gatcgcggcg tccgagacag ctggttccgc
    12661 gtacgcgagg aggaagtcgc ggaccgtggt cgggcgcacc tgcgagccga tgaactgccg
    12721 catcagcccc acccgaccgt cggtctggat cgagcccgcg gtcgggcggt cgtcgcccac
    12781 gatcaagcgg agcagcgtcg acttgccgat gccgttggcg ccgacgaggc cggcgtgctc
    12841 gccctcgggc acgcggaagc tcaccccctc gaaaagggtc cagcctcccg ggagggcgta
    12901 cgcgacgtcg gcgagctcga tgaaccccat gggcggtcca cgctagcagc cgccggaggg
    12961 cgttccgttc agcgtgctgc ggcgcgctcg tgttcgagcg agagctggcc gcacgccgcg
    13021 tcgatctcgc gtccgcgcgt gtcgcggatc gtcacggcga cgccggcgtc gtccaggatc
    13081 gaggcgaatg cgcggatccg cgaggccggg ctcggcttgg acggccaccc gggcgtcggg
    13141 ttcagcggga tcaggttgac gtgcgcccgc aggcggagcg cgatcggcgc gaggcgctcg
    13201 gcctgctcgt ccgcgtcgtt ggtccgatcg atcatcgccc actcgatcga cgggcgtcgg
    13261 cgcgtcacgc gacgccacgc ctcgatcgcg tcgaccagct cctcgagcgg ccacagctcg
    13321 ttgacgggga cgagccggtt gcgaaggcga tcgtcggccg cgtgcaggct gaccgccagc
    13381 ccgatctgtg ggtacgcctc ggcgaggcgg cggatcgccg gcgcgacgcc cacggtcgag
    13441 accgtgacgt gccggtcccc catccccatc tcgcccgtga gcagccccag ggcgccgaag
    13501 gtgtggcgct cgttcgacag cggctcgccc atccccatga agacggcgtt cgacaggcgg
    13561 cgaggggtgg acgcggggag tgcggccgcc tcccgcctgg cccagaccgc ctgcgccgcg
    13621 atctcgccgg cggtgaggtt gttgcgaagt cccatctgtc ccgtcgcgca gaacccacac
    13681 cccatcgcgc acccggtctg cgtcgagagg cacaccgtca cgcggtcccg atagcccatc
    13741 agcacggtct cgatcacgtg gcggccaccg atccgcagca gcgccttccg ggtcgcgccc
    13801 cggtcagcct gacgctcctc gagcacctcg accccgatcg ggagctcggc ctcgagccgc
    13861 tcgcgcaggc gcccggggac gtcggccatg cggtcgtacg tcgccgccct ctgccagagc
    13921 tgccggtgga cctgcctggc ccggaacggc ggctcgcccc agtcgccgag gcgctcggtg
    13981 agctggtcga gggtgaggtc gtaggcggtc gtcacgagga cgagcctacg aggatgcggc
    14041 tcctaccagt agcgagcggt cgacatcgcg aacgccgcga ccgacaccag cgccaggttg
    14101 aggatggcga ggctccgccc cctggcctgg agcgccgcca gctcctgcac cagccccgga
    14161 ggcggctggt cgccggcctg cgcgatctgg gcgccgaccg cgagcgaacg ctcgagcgcc
    14221 ggcttggtca gcgccccgcc gatcgcgacc gcgacgatcg ccgcgagcgc tccgatcgtg
    14281 agccagagcc cgaacgcgct gccgaggaag tcccccagac ctccgtacac ctggatgtcg
    14341 tgccaaaaga ggaatccgcc cgccacgatc gtcgccccgg cgatccgaag gagccagtcg
    14401 gtcaggtgac gcacgcccaa cagctcgcgc atgaacggac ctgccgcggg gccgatcgcc
    14461 ttggccgtcg gttgcaggaa gaccaccagc aggaacagcg ccccacccca cgcgatcccc
    14521 agcatcacgt ggatcacccg gaacaggacc atgtagatgc tcgtcgcgag cacgcccgca
    14581 cctctttcct cgtcggcaac ggtgcatcct cgccccggag ccggcgccgg tcaaccgcgt
    14641 aggctgggcc gcgtgaccgc cgaccccttc gcgcgtgtcc gggagacgca cgggaccctc
    14701 gtgctcgacg gcgggctcgc caccgagctc gtgcgtcgcg gcgccgacct gagggacccg
    14761 ctctggtccg cgaaggtgct cgtcgaggac cccgacgcga tccgccgcgt gcacgaggcg
    14821 tacttcgagg ccggggcgga cgtcgcgatc tcggccagct accaggcgac gttcgagggg
    14881 ctcgccggcc gcgggatcga acgcacccag gcggcaacgc tcatgcgtcg gagcgtcgag
    14941 ctggcgcgcg atgccgcgag cggagatcgg ttcgtcgcgg cgtcggtcgg cccgtacggg
    15001 gcgatgctcg gcaacggcgc cgagtacacg ggcgactacg acaaggacga ggacgagctc
    15061 gtccggttcc acgtgccccg gttggaggcg ctcgccgagg cggggccgga cgcgttcgcc
    15121 gtggagacga tcccctcgtt cgtcgaggcg ggagcgctcg cccgcgcgct cgagcgggtg
    15181 cccgagatcc cggcgtgggt cagcttcagc tgccgcgacg gagggcacgt ctgcgacggc
    15241 accccgatcg catcggccgt cgagatcgtg gcgcccgcgc cgagcgtgat cgccgtcggc
    15301 gtcaactgca cctcgccgct gcacgtcgag cggctcgtcg agacgatctc ggccacgacc
    15361 gacaagccgg tggtgtgcta tccgaaccgc ggatcgttct gggaccggat gcgcaagacg
    15421 tggaccgacc caccccgaca ggacgcgcgt ccgccgctcc ggccgctcgc gtggagggag
    15481 gccggcgcgt cgctgatcgg cgggtgctgc gggacgacac ccgaggacat cgccgcgatc
    15541 gcggcggcgc tcggccgcgc ctgagcgttc gctaaagggg cgtgtccggc cccggctcga
    15601 acggcgcccc atgccccgga acgatcgtgc gcagccgccg gagcgcgagc acgcgggcgc
    15661 gattctcgtg gagcacgctc gggtcgggcg cgaacggatc ttccgtgggg accgtcgcgg
    15721 tccaccacag atgcgtgaac accacgagcc cgtcgtccgt gtccacgagc gtggagacgt
    15781 cctgcggcga atgtcctggc gtctcgatca ggcggatcga cgaggacagc tcgaaccctt
    15841 ccgcgggcct gtcgacccac aggtcgtcgc ggtaccacgc ccagtggtcg tggaccctcg
    15901 cgtcggggaa cagcgccgtg ttcagcgtgt gatcgggatg gtggtgcgag agcaccacgt
    15961 cggtgatctg atcgggtgcg agctcgagct cggcgagcgg tcgcaggatc gcctcacgcc
    16021 ccggcacgag accgggatcg atcaccacga gcaccccgcc gtcccgcgcg aacccgaccg
    16081 tcgagcccac ccggtcgccg ccgtcgccga ggtagccctc gctcaggatg tggacctcgg
    16141 ccgtcatggg ctcacgccaa ctccaggtcg ggctgcgatg tcccacggta cgccatccgt
    16201 tcgggccgaa cagccgtggc ctcggcgccg ccggcccacg cggatccggc cggctctgac
    16261 cgcgcgcggt cagaccgaga tccgactcct cggcaggaac cgcccctggc cgggcttgcc
    16321 gtggaaggtc ccgtcctgca cgagcacctg cccccgctgg agcacgacct cgggcagacc
    16381 gcgcaccttc cggccctcgt acgccgagta gtcgacgttc atgtggtggg tcttcgccga
    16441 gatctcgcgc tcgacccgcg gatcgaacac cacgacgtcc gcgtccgaac ccggcgcgat
    16501 cgtgcccttg cgcgggtaca ggccgaacat cttggccggc gccgtcgcca cgatctccac
    16561 gaagcggttc aacccgatgt ggccggcctc gacgccgcca tacaacagcg tgaagcggtc
    16621 ctccaccgac gggagaccgt tcgggatctt ggagaagtcg tccttgccga gatccttctg
    16681 ccccacgtag ttgaagggcg cgtggtcggt cgagacgatc tgcaggtcgt cgccgcgcag
    16741 gcccttccac agctcctcct ggtggtcggc cggccggagc ggcggcgagc acacgaactt
    16801 cgcgccctcg aaccccggcc gacccaggtc gtcgagggac aggtacaggt actgcggaca
    16861 ggtctcggcg aacgcgggca tgccccggtc ccgcgcctcg cgaaccgcg
//
LOCUS       DATBRK010000411         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_376658, whole genome shotgun sequence.
ACCESSION   DATBRK010000411 DATBRK010000000
VERSION     DATBRK010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376658"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VIH70_12190"
     CDS             <1..343
                     /locus_tag="VIH70_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8117168.1"
                     /translation="LRDLQKTDDQWARAKGFDGSCPLGPWIETDLDPNDVRVEARVNG
                     EVRQAGQTSEMVFGVATLIEYVTSFMTLLPGDVILTGTPEGVGPLSAGDLVEVEVDGV
                     GTLANPVEAGT"
     gene            366..1784
                     /gene="gltX"
                     /locus_tag="VIH70_12195"
     CDS             366..1784
                     /gene="gltX"
                     /locus_tag="VIH70_12195"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY8117169.1"
                     /translation="MTAGVRTRFAPAPSGSIHVGNARTGLFSWAFARHHAGAFVLRIE
                     DTDASRVTEEAFHSVMDSLRWLGIDWDEGPDIGGPHEPYRQSRRLGIYREMTQRLLSQ
                     DDAYPCYCTEEELEERREAALARGEAPGYDGKCRDPEVRRAFEAEGRTAAIRFRMPER
                     EHVIDDLVKGEVRWPPGQLRDFVIVRSDGTPVFLLAVAVDDMLMAITHVIRGDDLLAS
                     APRNAAVIEALGGTPPAYAHVPQVNGGDGKPLSKRHGSTSVEAFREHGILPEALMNYL
                     ALLGWSKDAETTFLSKQELIDAFELERVSRNPARFDVEKLEWMNNHYVQSLDDDDLAS
                     RCLHFLSVAGLSPDLELLRRAMPLVRKRMKRLSDSVQLLRFLFTDDIAADDEASGVIS
                     KAPDGYLKEVADGLADVRPWEADGIKARLDELAAAAGLNRTKGFQPVRAAVTGSTVSP
                     PLPESLELLGRERTLERLRAVP"
     gene            1781..2491
                     /locus_tag="VIH70_12200"
     CDS             1781..2491
                     /locus_tag="VIH70_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEY8117170.1"
                     /translation="MRRAALLLVSALLLAAPESAAIAVGVPDPGDVKPQIVWKKIPFG
                     ATRKRQMARYSERHYGERTYELTDPRVVVEHYTDGTSFDSAWNHFAANGTHLGEKPGV
                     CAHFLIDTDGTIYQLVNLRIRCRHAIGMNWTAIGIEHVGTSAGAILGDHDMMRGSLRL
                     TVWLMSRYGISWGNVIGHAETLQSPFHHELYADWRCLTHADFNRSEMRIYRRRLTRLA
                     ERLGVPVGAGPRWVDSGC"
BASE COUNT          375 a          849 c          920 g          359 t
ORIGIN      
        1 cctccgcgac ctgcagaaga ccgacgatca gtgggcgcgc gcgaaggggt tcgacgggtc
       61 gtgcccgctg gggccgtgga tcgagaccga cctggacccg aacgacgtgc gggtcgaggc
      121 gcgcgtgaac ggcgaggtcc gtcaggcggg acagacgagc gagatggtgt tcggcgtcgc
      181 gacgttgatc gagtacgtca cgtcgttcat gacgctgctg ccgggcgacg tgatcctcac
      241 ggggaccccg gagggagtcg gcccgctctc ggccggcgat ctggtcgagg tcgaggtcga
      301 cggtgtgggc acgctcgcca acccggtgga ggcgggcacg tgaccggccg cgtggagcgc
      361 cccggatgac cgcgggcgtg cggacgcggt tcgcgccggc gccgtccggg tcgatccacg
      421 tcgggaacgc tcggacgggg ctgttctcgt gggcgttcgc gcgccatcac gcgggcgcgt
      481 tcgtccttcg gatcgaggac acggacgcct cgcgggtcac cgaggaggcg ttccactccg
      541 tgatggactc gctccggtgg ctcgggatcg actgggacga ggggcccgac atcggcgggc
      601 cgcacgagcc gtaccggcag tcgcggcgcc tcggcatcta ccgcgagatg acgcagcggc
      661 tcctgtccca ggacgacgcg tacccgtgct actgcacgga ggaggagctc gaggagcggc
      721 gcgaggccgc gctcgcgcgc ggtgaggcgc ccggctacga cggcaagtgc cgtgacccgg
      781 aggtgcgcag ggccttcgag gccgagggcc gcaccgcggc gatccgtttc cggatgccgg
      841 agcgcgagca cgtgatcgac gacctcgtga agggcgaggt ccggtggccg ccgggtcagc
      901 tccgcgattt cgtgatcgtg cgttcggacg gcaccccggt gttcctgctc gcggtcgcgg
      961 tcgacgacat gctgatggcg atcacgcacg tgatccgcgg cgacgacctg ctcgcgagcg
     1021 cacctcgcaa cgccgcggtg atcgaggcgc tcgggggcac gccccccgcg tacgcgcacg
     1081 tcccgcaggt gaacggcggg gacggcaagc cgctgtccaa gcgccacggt tccacgagcg
     1141 tcgaggcgtt ccgcgaacat gggatcctgc cagaggcgct catgaactac ctcgcgctcc
     1201 tcggctggtc caaggacgcg gagacgacgt tcctgtccaa gcaggaactg atcgacgcgt
     1261 tcgagctcga gcgcgtctcg aggaacccgg cccggttcga cgtcgagaag ctcgagtgga
     1321 tgaacaacca ctacgtccag tccctggatg acgacgacct cgcgtccagg tgcctgcact
     1381 tcctctcggt cgccggcctg tcgcccgacc tcgagctcct gcgccgcgcg atgccgctgg
     1441 taaggaagcg gatgaagagg ctgtcggact cggtgcagct gctccggttc ctgttcaccg
     1501 acgacatcgc cgcggacgat gaggcctcgg gggtgatctc gaaggcgccg gacggctacc
     1561 tgaaggaggt cgccgacggg ctcgccgacg ttcggccgtg ggaggccgat gggatcaagg
     1621 caaggctcga cgagctcgcc gccgcggccg gcctgaaccg cacgaagggg ttccaacccg
     1681 tccgggccgc ggtgaccggg tcgacggtct cgccgccgct gcccgagtcc ctcgagctcc
     1741 tcggccgcga gcgcaccctc gagcgcctcc gggcggtccc atgaggcgcg cggccctcct
     1801 cctggtctcg gcgttgctcc tggccgcgcc cgagtcggcc gcgatcgcgg tcggggtccc
     1861 ggacccgggc gacgtgaagc cccagatcgt gtggaagaag atcccgttcg gcgcgacccg
     1921 caagcgacag atggcccggt actccgagcg ccactacggc gaacgcacgt acgagctcac
     1981 ggatccccgg gtggtcgtcg agcactacac cgacgggacc tcgttcgact ccgcgtggaa
     2041 ccacttcgcc gcgaacggca cgcacctggg cgagaagccg ggggtctgcg cgcacttcct
     2101 gatcgacacc gacgggacga tctaccagct cgtgaacctg cggatccggt gccgccacgc
     2161 gatcgggatg aactggaccg cgatcgggat cgagcacgtc gggacgagcg ccggggcgat
     2221 cctcggcgac cacgacatga tgcgggggtc gctccgtctg acggtgtggc tgatgtcgcg
     2281 ctacgggatc tcctggggca acgtgatcgg gcacgccgag acgctccaga gcccgttcca
     2341 ccacgagctg tacgcggact ggcgatgcct gacccacgcc gacttcaatc gatcggagat
     2401 gcggatctac cgccgcaggc tcacgcgcct cgcggaacgg ctgggggtgc ccgtcggcgc
     2461 cgggccgcgc tgggtcgact cgggctgcta ggcgttggcg agc
//
LOCUS       DATBRK010000412         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_675224, whole genome shotgun sequence.
ACCESSION   DATBRK010000412 DATBRK010000000
VERSION     DATBRK010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675224"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..421)
                     /locus_tag="VIH70_12205"
     CDS             complement(140..421)
                     /locus_tag="VIH70_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018223277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY8117171.1"
                     /translation="MGLVTVRVVPKAGRASVGLDDRGIVVRVRAAPEGGRATEEARRA
                     LAEALGVPGSAVSLHRGTRSRAKVFEIAGVGQPEAELRLRTTEADSLPP"
     gene            733..>2054
                     /locus_tag="VIH70_12210"
     CDS             733..>2054
                     /locus_tag="VIH70_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY8117172.1"
                     /translation="MFEPVDRKANFPELERGVLGFWREAEVFHRQLEQRRGEPLWIFY
                     EGPPTANGQPAIHHTESRTFKDVYPRFRAMTGHFVPRKAGWDCHGLPVELEVEKEIGV
                     KSKRDIEAFGVAEFNRLCRESVQRYVGEFERQTERLGFWIDTDDAYWTMDTEYIESVW
                     WSLKHLHGRGLLFQDDRITTYCPRCGTPLSDAEVAMGYAEVEDPSVFILFRIVEATDP
                     PLVGASMLGWTTTPWTLISNTGLAVKDDASYVVVELDGERLVVAEALRDAVLPDAPVV
                     AGPLPGSAFVGIRYEPLYPNVEGAHRVVAADFVSLEDGTGVVHLAPAFGSEDLEIGRR
                     EGWPTFKPLDGEGRFTDEAPALVRGAFFKDADEAIVGDLRSRGLLLRAGTIVHAYPLC
                     WRCDTPLIYIARGSWYVGTTAVKDRLLAVNEEVDWYPSHIKHGRYGDWL"
BASE COUNT          299 a          696 c          757 g          302 t
ORIGIN      
        1 aacgaggtct cctcgatggt cgtcagttgc acctgcagct cagcccgttc ggcctccagg
       61 cgctggcgca gctcgtccag ttccttcttg ctgtgggtga tcttcccggc catggtcgga
      121 ggaacctagc atcggcggct cagggcggca atgagtcggc ctccgttgtc cgaaggcgca
      181 actccgcctc gggttggccg acgccggcga tctcgaagac cttggcccgg gagcgggtcc
      241 cgcggtgcag ggagaccgcg gaccccggaa cgcccagcgc ctcggcgagc gcccgccggg
      301 cctcctccgt ggcccggccg ccctcgggcg cggcgcggac gcgcaccacg atcccccgat
      361 cgtcgagtcc gaccgaggct cggccggcct tcgggaccac gcgcaccgtg accagaccca
      421 tcccggggtg tagcatcgcc cccgaaggcg atgacgggaa cgagtacgag ggatcccgag
      481 cctgagcgag ccggggacgg tgtgagcccg gcggcgaggc ccctccgaac atcctcctcg
      541 agccgccgcc ccaaccgggc gcgcgccacg cgcccgcagt agacgcggcc gggtccgccc
      601 gtgacagcgg tgggctgtgg atgcagccga tgagcggccg ggcctgcggg cccggcaacg
      661 cggggtggta ccgcggggcc cacgcctcgt ccctgcaacg gggacggggc gttcggcgtt
      721 tcggaggtgg cgatgttcga gccggtcgat cggaaggcga acttccccga gctcgaacgc
      781 ggggtgctcg ggttctggcg cgaggccgag gtgttccacc gccagctcga gcagcgccgc
      841 ggcgagccac tgtggatctt ctacgagggg ccgcccaccg cgaacgggca gccggcgatc
      901 catcacacgg agtcgcgcac cttcaaggac gtgtatcccc gcttccgggc gatgacgggg
      961 cacttcgtcc cgcgcaaggc ggggtgggac tgccacgggc tgccggtcga gctcgaggtc
     1021 gagaaggaga tcggcgtcaa gagcaagcgg gacatcgagg cgttcggggt cgcggagttc
     1081 aaccggctct gccgcgagtc ggtccagcgg tacgtgggcg agttcgaacg ccagacggaa
     1141 cgcctcggct tctggatcga caccgacgac gcgtactgga cgatggacac cgagtacatc
     1201 gagagcgtct ggtggtcgct caagcacctc cacggaaggg gattgctgtt ccaggacgat
     1261 cggatcacga cctactgccc gcgatgcggc acgcccctgt ccgacgcgga ggtcgcgatg
     1321 ggctacgccg aggtggagga cccgagcgtc ttcatcctgt tccgcatcgt cgaggcgacg
     1381 gacccaccgc tcgtcggagc ctcgatgctc gggtggacga ccacgccgtg gacgctgatc
     1441 tccaacacgg ggctcgcggt gaaggacgac gcctcgtacg tggtggtcga gctggatggg
     1501 gagcggctgg tcgtggccga ggcgctgcgc gacgccgtcc tgccggatgc gccggtcgtg
     1561 gccggcccgt tgcccgggag cgcgttcgtc gggatccggt acgagccgct gtacccgaac
     1621 gtcgagggcg cccatcgcgt cgtggcggcc gacttcgtct ccctcgagga cggcaccggc
     1681 gtcgtccacc tggcgcccgc cttcggctcc gaggacctcg agatcggcag acgggagggg
     1741 tggccgacgt tcaagccgct cgacggcgag ggccggttca cggacgaggc tccggcgctc
     1801 gttcgcggcg ccttcttcaa ggacgcggat gaggccatcg tcggcgacct gcggtcgcgt
     1861 gggctgctgc tgcgggcggg gacgatcgtc cacgcctatc ccctgtgctg gcgatgcgac
     1921 acgccgctga tctacatcgc caggggctcc tggtacgtgg ggacgaccgc ggtcaaggac
     1981 cggctgctcg cggtgaacga ggaggtcgac tggtatccgt cgcacatcaa gcacgggcgc
     2041 tacggcgact ggct
//
LOCUS       DATBRK010000413        11529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1393390, whole genome shotgun sequence.
ACCESSION   DATBRK010000413 DATBRK010000000
VERSION     DATBRK010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11529
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393390"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VIH70_12215"
     CDS             complement(<1..247)
                     /locus_tag="VIH70_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEY8117173.1"
                     /translation="MRTVFRGARWPGDVAVRDGRIEAVGAVAEEPGDEVIRCDGDLIT
                     AGLVNTHHHLYQWMTRGRCVGCDLFGWLVELYPVWGRL"
     gene            290..1621
                     /gene="allB"
                     /locus_tag="VIH70_12220"
     CDS             290..1621
                     /gene="allB"
                     /locus_tag="VIH70_12220"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEY8117174.1"
                     /translation="MHGPDVLIRGRRVVTPGGVREACINVVDGRIERVASFDDAPSGV
                     PLVDAGDRTVMPGLVDTHVHVNEPGRTEWEGFETATRAAAAGGVTTLLDMPLNSIPPT
                     TTIAALAEKRAAADGKVHVDVGFWGGAVPGDLGRLRELHDAGVFGFKCFLCPSGVEEF
                     SSLDLDQLAVAMGEVAAFDGLLIVHAELPEPIERATDGLAGSDPFAYRTYLALRPASA
                     EEQAIAAVAEASAATGCRTHVLHLSAASCVDLLGDVTAETCPHYLTFAAEEIADGATE
                     YKCAPPIRDAENRERLWEGLRDGRISAVVSDHSPCTADLKTGSFFQAWGGIASVELGL
                     RAVWTQARARGFGLEDVARWMSEGPAAIAGLERKGAIAEGRDADLVLFEPDAASVVDP
                     IELHHRNPITPYAGRRLDGRVAATYVRGVEVYADGRFRDEPAGRLLSRGAA"
     gene            1618..3111
                     /gene="alc"
                     /locus_tag="VIH70_12225"
     CDS             1618..3111
                     /gene="alc"
                     /locus_tag="VIH70_12225"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEY8117175.1"
                     /translation="MSDFTTLPDLAARRLGGMVLVANDEFFAPKENLVKPEAPAFDPD
                     RYTDRGKEMDGWETRRRREPGHDWCIVRLGVPGIVRGVVVDTSFFRGNYPDRCSVEGA
                     VVEEDDVTGADWFDLVPESALEGDAVNRFDVASPLRVSHLRLNIVPDGGVARLRVHGE
                     PLPDLRTLTDAGGRADVAASLSGGVVLDASDLFFSSPHNLIAPGDSHGMHDGWETRRR
                     RGEGHDWVAIRLATETEIERIEVDTSNFKGNYPDTCSLEVRVSPVAGSEPEAEPAWNE
                     VLSHAKLGPHRRFTFPIDPPVAATQVRLNIHPDGGVARLRVHGPVTDYGWRSFGVRWL
                     NAATPEAFEEDVLGCCGSRAWASGLRAARPFATFDAMLQAADDVWGGLPADDRREAFA
                     AHPRIGERGGSVWARGEQAGAAEASDEILRALEAGNHEYEERFGHVFLINASGKGAEE
                     MLEALRERLGNDPDAELAEAAEQQRQITRIRLEKLVRPVAPLVRAGG"
     gene            3119..3460
                     /gene="uraH"
                     /locus_tag="VIH70_12230"
     CDS             3119..3460
                     /gene="uraH"
                     /locus_tag="VIH70_12230"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY8117176.1"
                     /translation="MAAITTHVLDTSAGRPATGVAVELEVLAAGGWEPVGAGLTDEQG
                     RVSGLLPAGADPMAATYRMRFDTGAYFGHQGVETFFPAVTVEFTLRDPGEHHHVPLLL
                     SPFGYSTYRGS"
     gene            3460..4323
                     /gene="pucL"
                     /locus_tag="VIH70_12235"
     CDS             3460..4323
                     /gene="pucL"
                     /locus_tag="VIH70_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEY8117177.1"
                     /translation="MGFELGPNRYGKAGIHLATVVRGEDRHEFSEREIEVRLEGDFDG
                     VHTVGDNAAVLPTDTMRSSAYALAFEHPEEALEAFALRYTEYLLHASPAASRAEAWMS
                     ERPWERVVIDGQPHPHAFTRGAFRWTTHVVRDRDDVRLSGGLDELYLLKTTGSAFSGF
                     LKDRYTILPETDDRILATKLDARWRYTRTDLGFAAERDACRDALVRAFAHHDESRSVQ
                     HTLWHMGTAVLEAAPNVEDVTLSMPNLHHVAVDLSYCGLNNDGKVFVVTDKPSGRIEG
                     TVRRTEDVVSG"
     gene            4320..5573
                     /locus_tag="VIH70_12240"
     CDS             4320..5573
                     /locus_tag="VIH70_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY8117178.1"
                     /translation="MSRSFPDDAWRDLLERARRAAAEHRRRFPGDLGAGRPLETLYVP
                     ADRLTATTAVEFGAEALRLLDAHAPDPASFASAFGVDAAVAKRARARVRAKLEHAPVE
                     DLRVDFEDGYGARPDDEEDRHAEVTARAVRAGRADGTAPPSFGLRVKSFAAGAEARSL
                     RTLDTFLTTLTEGAAGLPDGFVVTFPKVTTAEHVALFAEALERLEVGLGLPERSLRFE
                     VQVETPRSVIGPDGSVAAPGILEAAGGRLTAMHFGVFDYTASLGLMPWEQRLDHPAND
                     FARHALQVALAGTGVWVSDGSSNVVPATDRTEDVHAAWRVHADHVRHSLRHGFYQGWD
                     LHPAHLVSRYAAVYGWLLPHLDDAASHARAWRRRVEAPGVLDEPATVGSLLRYLRFAV
                     SSGAVDEAEVLEASGLARDELLGSG"
     gene            5584..7176
                     /gene="aceB"
                     /locus_tag="VIH70_12245"
     CDS             5584..7176
                     /gene="aceB"
                     /locus_tag="VIH70_12245"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEY8117179.1"
                     /translation="MTHPEGVEVLGPAEPRFDEILTDEALAFVAGLQREFGGRRDELL
                     EARAERQVRLDKGGRPDFLEATRTVRQADWRVAPAPPDLRDRRVEITGPTDRKMVINA
                     LNSGASCFMADLEDANSPTWSNMVGGQVNLADAVRRTIELTGPEGREYRLTEHPATLL
                     VRPRGWHLPERHMVVDGALVSGSLFDFGLAFFHNAREQLERGTGPYFYLPKLEGHLEA
                     RLWNDVFLWAQDRLAIARGTIRATVLIETILAAFEMDEILHELRDHSAGLNAGRWDYI
                     FSVIKKFRRHPDFVLPDRARISMTVPFMRAYTELLVRTCHRRGAHAMGGMAAYIPSRR
                     DPEANELALAKVAEDKVREANDGFDGTWVAHPDLVGTAAAEFDRVLGDRPNQVDRQRD
                     DVGVSADQLLDVRVPGGEITQAGLRANVNVGLRYLMSWLSGVGAAAIDNLMEDAATAE
                     ISRSQIWQWVEHGAELSTGQPVTAEVAHAAITTELEDLRERLGSAEFDEPRFEQARTV
                     FERVALSPDFVEFLTLPAYELL"
     gene            7261..7767
                     /locus_tag="VIH70_12250"
     CDS             7261..7767
                     /locus_tag="VIH70_12250"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY8117180.1"
                     /translation="MRRAIIVALLAALALGVVATSAPAQPSSFRAVLSGAGEVPARDT
                     PARGVAIFNLNADGTELGFQLVVANIENVFAAHIHCGVAGVSGPIGVTLFMGAPGGGR
                     TDGVLAAGSVTAPDAGNACGWATLADVVAAVEAGNAYVNAHTNDGVAPPNTGPGDFPG
                     GEIRGQVF"
     gene            complement(7775..8683)
                     /locus_tag="VIH70_12255"
     CDS             complement(7775..8683)
                     /locus_tag="VIH70_12255"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY8117181.1"
                     /translation="MTPLEIPDEVVRMNLSANGAAGRAWLGSLPGLVEELAGRWSLTV
                     GPAFAGGAVGFVAPAERRGGERLVLKVSFVDEETQHEPDALRHWDGDGAVRLVDAEPA
                     RGALLLERLEPGTPLADHPDRSEAIGIACSLLRRLWRPVPGDHPFSLVRDLASRLADE
                     LPGRFERQGRPFDERLLRTAVDLCRDLAGSSEEAVLANRDFHLWNVLAAEREPWLVID
                     PKPLVGERAFDTAHLVRTLLPERLDLGSVDAIVGRLANELELEPTHVRRWAFVRSLDD
                     ALWGLSTGFTDVGWDVRCADLIAERF"
     gene            complement(8680..10131)
                     /locus_tag="VIH70_12260"
     CDS             complement(8680..10131)
                     /locus_tag="VIH70_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8117182.1"
                     /translation="MAARCDELVKTYRTASGEVRALKGLSAEVPASSLLAVVGPSGSG
                     KSSLLRLIAGLDRPTSGTLMVEGTSVHDASVRTLRRFRRATVGYLFQRPSDNFLPHLT
                     VGEHLRLARGLTHRPPRIDQEALLSTLGIEERVDHLPSELSGGEQQRAAIAEVLMGGA
                     TIVVADEPTAELDSTSASRVMDTIVELARAGVTFVVATHDRSVMRRADAALELDHGMR
                     RSSTTAPAEPADRSSAAAFRRAPAAAEPDRADRWRPPMTTTAPERERPFVLDANGLSK
                     AYRRGDEVVHALDDVSLTLRAGELVGLVGRSGSGKTTLLNVIAGWEHADAGTIQRSGG
                     RSATPGWDELAVVPQKLGLFDELSVRENLEYPARLHGRLEELDARVDELMEDLGIAHL
                     AVRYPKETSLGEQQRTAVARALVLSPTLLLADEPTGHQDAVSERRVLEAFGRAAAEGG
                     SCLIATHNVGLAPRLDRVLEMADGRLGAPPSTP"
     gene            complement(10160..>11529)
                     /locus_tag="VIH70_12265"
     CDS             complement(10160..>11529)
                     /locus_tag="VIH70_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117183.1"
                     /translation="VGAFPWELVLIGLSLLILQRLRSGGAVVVDRSLGTETPSLLLFA
                     FPLVFLAGFVALVARAMVLVLRWARGRSGSWGTPAYLAVHRLAARSRLTLLLIAAAGL
                     CLGLFVQAQTMARSMRTTVDAKAGVYVGSDVEARIDSVNATPETFPLPVTRVVRRLQA
                     GELRPGVPFDLLAIDPKTFASTAFWDPGFSPEPLETLLDRISKPSGGGLPVLMASAPN
                     AFPDSITLDRQTLPIEIVGRATAFPGMTSIRPLVVVDAQRLLDAFGDAPDPLSGPSAS
                     NELWIRGTPRAARAALAELDYTPSLVLTADEVKDISYIAAVIDTFLVMNGLGVLAALL
                     VVAAMLMYLQARQRAEIVSYGLSLRMGMRSSGHLVAIAMEVGAMLVIAYVAGTVLAIL
                     AARLTVPLLDPIESIPPDPITVVPVALVALTAPALLLVALAGGWLTERRARSADLGQV
                     MRLAD"
BASE COUNT         1661 a         4073 c         4245 g         1550 t
ORIGIN      
        1 cgagacgccc ccagaccggg tagagctcga cgagccagcc gaacaggtcg cacccgacgc
       61 accggccgcg cgtcatccac tggtagaggt ggtggtgggt gttcacgagc cccgcggtga
      121 tcaggtcgcc gtcgcaccgg atcacctcgt cgccgggctc ctcggccacc gctcccaccg
      181 cctcgatccg accgtcgcgg acggcgacgt cgcccggcca ccgcgcccct cggaagacgg
      241 ttctcacggc ggtgagcgta gacgttcggg gccgtgccac gatgcgatcg tgcacgggcc
      301 cgacgtcctg atccgaggcc gccgcgtcgt gacgccgggc ggtgttcgcg aggcgtgcat
      361 caacgtcgtg gacggacgca tcgagcgcgt cgcgtcgttc gacgacgcgc cgtcgggtgt
      421 cccgctcgtg gacgccggcg accgcacggt gatgcccgga ctggtcgata cgcacgtgca
      481 cgtcaacgag cccggacgga ccgagtggga ggggttcgag accgcgacgc gagcggccgc
      541 ggcgggcggc gtgacgacgc tgctcgacat gccgctcaac agcatcccgc cgacgacgac
      601 gatcgccgcc ctcgccgaga agcgggccgc cgccgacggg aaggtccacg tcgacgtcgg
      661 gttctggggt ggggcggtcc cgggggatct cgggcggttg cgcgagctcc acgacgccgg
      721 cgtgttcggg ttcaagtgct tcctctgtcc gtccggggtc gaggagttct cgtcgctcga
      781 tctcgatcag ctcgcggtgg cgatggggga ggtcgcggcg ttcgacgggc tgctgatcgt
      841 gcacgccgag ctgcccgagc cgatcgagcg cgcgacggat gggctcgccg gctccgaccc
      901 gttcgcgtac cgcacctacc tcgcgttgcg gccggcctcg gcggaggagc aggcgatcgc
      961 ggcggtcgcc gaggcaagtg cggcgacggg gtgccggaca cacgtgctcc atctctcggc
     1021 ggcgtcgtgt gtggatcttc tcggcgacgt gacggccgag acgtgccccc actacctgac
     1081 cttcgcggcc gaggagatcg cggacggcgc gaccgagtac aaatgcgcgc ctccgatccg
     1141 cgacgccgag aaccgggagc gcctgtggga ggggctccgg gacgggcgga tctcggcggt
     1201 ggtgtccgat cactcgccct gcaccgccga cctcaagacg ggctccttct tccaggcgtg
     1261 gggcggcatc gcctccgtgg agctcgggct gcgggccgtg tggacgcagg cccgggctcg
     1321 ggggttcggg ctcgaggacg tggcccgctg gatgagcgag gggccggccg cgatcgccgg
     1381 gctcgagcgc aagggggcga tcgccgaggg cagggacgcg gacctggtcc tgttcgagcc
     1441 agacgcggcc tccgtcgtcg accccatcga gctccaccac cgcaacccca tcacgcccta
     1501 cgcgggccgc cggctcgacg gacgcgtcgc cgccacgtac gttcgagggg tggaggtcta
     1561 cgcggacggg cggttccggg acgaaccggc cggccgcctg ctgtcgaggg gagcggcgtg
     1621 agcgacttca cgacgctgcc cgacctcgca gctcggcgcc tgggcgggat ggtgctcgtc
     1681 gcgaacgacg agttcttcgc gccgaaggag aacctcgtga agccggaggc gccggccttc
     1741 gaccccgacc gctacacgga tcgcggcaag gagatggacg gctgggagac gcgccgccga
     1801 cgcgagccgg gccacgactg gtgcatcgtc cgcctcggcg tccccgggat cgtccgcggc
     1861 gtggtcgtcg acacgagctt cttccgcggc aactacccgg accgctgctc ggtcgagggc
     1921 gcggtggtcg aggaggacga cgtcacgggc gcggactggt tcgacctggt gcccgagtcg
     1981 gcgctcgagg gcgacgcggt caaccggttc gacgtcgcct cgccacttcg ggtgagccac
     2041 ctgcggctca acatcgtccc cgacggcggg gtcgcgaggc tccgcgtcca cggcgagccc
     2101 ctcccggatc tgcgaacgct caccgacgcc ggcggtcgcg cggacgtcgc ggcgtcgttg
     2161 tccggtgggg tcgtgctcga cgcgagcgat ctcttcttct catcgcccca caacctgatc
     2221 gcgccgggcg actcccacgg catgcacgac gggtgggaga cgcgacgccg ccgaggcgag
     2281 ggccacgact gggtggcgat ccgcctcgcg accgagaccg agatcgagcg catcgaggtc
     2341 gacacctcga acttcaaggg caactacccc gacacgtgct cgctcgaggt tcgtgtgtct
     2401 cccgtcgccg gctccgaacc ggaggccgag ccggcctgga acgaggtcct ctcgcacgcc
     2461 aagctgggcc cgcatcgacg cttcacgttc ccgatcgacc cgccggtggc ggccacacag
     2521 gttcggctga acatccatcc cgacggaggt gtggcccggc tccgggtaca cgggccggtc
     2581 accgactacg gctggcgctc gttcggcgtc cgatggctca acgcggcgac gcccgaggcg
     2641 ttcgaggagg acgtgctcgg ctgctgcggc tcgcgcgcct gggcgagcgg gctccgggcg
     2701 gcgaggccgt tcgccacgtt cgacgcgatg ctgcaggccg ccgacgacgt gtggggcgga
     2761 ctcccggccg atgatcggcg tgaggcgttc gcggcgcacc cgcggatcgg ggagcgaggc
     2821 gggtccgtct gggctcgggg cgagcaggcc ggcgccgccg aggcgagcga cgagatcctc
     2881 cgcgcgctcg aggccggcaa ccacgagtac gaggagcggt tcgggcacgt gttcctgatc
     2941 aacgcctccg gcaagggcgc cgaggagatg ctcgaggcgc ttcgcgagcg gctcgggaac
     3001 gaccccgacg ccgagctcgc cgaggccgcc gaacagcaac ggcagatcac ccggatccgg
     3061 ctcgagaagc tcgtccggcc cgtggcgccg ctcgttcgcg cgggaggatg atgcgccggt
     3121 ggcggcgatc acgacccacg tgctcgacac ctcggccggc cggcccgcca ccggcgtggc
     3181 ggtcgagctc gaggtcctcg ccgccggcgg ctgggagccg gtcggggcgg gtctgaccga
     3241 cgaacagggc cgggtgagcg gcctgctccc cgcgggcgcc gacccgatgg cggcgacgta
     3301 ccggatgcgg ttcgacacgg gcgcgtactt tggccaccaa ggcgtggaga ccttcttccc
     3361 cgcggtgacg gtggagttca ccctgcgcga tcccggggag caccatcacg ttccgttgct
     3421 gctcagcccg ttcggctact ccacctaccg ggggagctga tggggttcga gctcgggccc
     3481 aaccggtacg gcaaggccgg gatccatctc gcgaccgtgg tgcggggcga ggatcgtcac
     3541 gagttctccg agcgcgagat cgaggtccgc ctggagggcg acttcgatgg ggtccacacc
     3601 gtaggcgaca acgccgcggt gctcccgacc gacacgatgc ggagctcggc gtacgcgctc
     3661 gcgttcgagc atcccgagga ggcgctcgag gctttcgcgc tccggtacac ggagtacctg
     3721 ctgcacgcgt cccccgctgc gagccgggcc gaggcgtgga tgtccgaacg gccctgggag
     3781 cgcgtcgtga tcgacgggca gcctcacccc cacgcgttca cgcgcggagc cttccgatgg
     3841 acgacgcacg tcgtccgcga ccgagacgac gtccggctgt ccggcgggct cgacgagctc
     3901 tacctgttga agacgaccgg gtcggcgttc agcggcttcc tcaaggaccg gtacacgatc
     3961 ctgccggaga ccgacgaccg gatcctcgcg accaagctcg acgcgcgctg gcggtacacg
     4021 cgaacggacc tcggcttcgc cgccgagcgg gacgcgtgcc gggacgcgct cgtccgcgcg
     4081 ttcgcccacc acgacgagag ccgctcggtg cagcacaccc tctggcacat gggcaccgcg
     4141 gtgctcgagg cggcgccgaa cgtcgaggac gtgacgttgt cgatgccgaa cctccaccac
     4201 gtcgcggtcg acctctcgta ctgcgggctc aacaacgacg ggaaggtctt cgtcgtgacc
     4261 gacaagccat cgggtcggat cgaggggacg gtgcgacgga ccgaggacgt cgtctcgggg
     4321 tgagtcgttc gttccccgac gacgcgtggc gggacctcct cgagcgcgcc cgtcgggccg
     4381 cggccgagca ccggcgccgg ttccccggcg acctcggcgc cggccggccg ctcgagacgc
     4441 tctacgtccc cgccgaccgc ctgacggcga cgacggcggt cgagttcggg gccgaggcgc
     4501 tgcggctgct cgacgcgcac gcgcccgacc cggcgtcgtt cgcgtcggcg ttcggcgtgg
     4561 acgccgcggt cgcgaagcgg gcgcgtgcgc gcgtgcgcgc gaagctggag cacgcgccgg
     4621 tcgaggacct tcgcgtcgac ttcgaggacg gctacggcgc gcgacccgac gatgaggagg
     4681 accggcacgc ggaggtgacg gcccgagccg ttcgggcggg gcgcgcggac gggaccgccc
     4741 cgccatcgtt cggcctgcgg gtgaagtcgt tcgccgccgg cgccgaggcg cggagtctcc
     4801 ggacgctcga caccttcctc acgacgctca ccgagggcgc cgccggcctc cccgacggct
     4861 tcgtcgtcac gttcccgaag gtcacgaccg ccgagcacgt cgcgctcttc gccgaggcgc
     4921 tcgaacgcct tgaggtcggg ctcggtctgc ccgagcgctc gctgcgtttc gaggtgcagg
     4981 tcgagacgcc gcgatccgtg atcggcccag acggctcggt cgcggcgccc ggcatcctcg
     5041 aggcggcggg cgggcggctg acggcgatgc acttcggggt gttcgactac accgcgtccc
     5101 tcggactgat gccgtgggag cagcggctcg atcacccggc caacgacttc gccagacacg
     5161 cgctgcaggt ggcgctagcg gggaccggcg tgtgggtcag cgacgggtcg agcaacgtcg
     5221 tgccggcgac cgatcgcacc gaggacgtcc acgcggcatg gcgtgtgcac gccgaccacg
     5281 tccggcactc gctccgtcac ggcttctacc aggggtggga tctgcatccc gcgcacctgg
     5341 tgagtcgcta cgcggccgtc tacggatggc tgctcccgca cctggacgac gccgcgtccc
     5401 atgctcgtgc gtggcgccga cgggtcgaag cccccggcgt gctcgacgag ccggcgacgg
     5461 tcggcagcct gctccggtac ctgcggttcg cggtctcctc cggcgccgtc gacgaggccg
     5521 aggtcctcga agcctccggg ctcgcgcgtg acgagctgct cgggagcgga taggagggcc
     5581 ggtgtgaccc atcccgaggg cgtcgaggtc ctgggtcccg cggagccccg gttcgacgag
     5641 atcctgaccg acgaggcgct cgcgttcgtc gccggcctgc agcgcgagtt cggcgggcgt
     5701 cgcgacgagc tgctcgaggc ccgcgccgaa cgccaggtcc ggctcgacaa gggggggcgg
     5761 ccggacttcc tcgaggcgac gcgtacggtc cgccaggcgg actggcgggt cgcacccgcg
     5821 ccgcccgacc ttcgggaccg cagggtcgag atcaccgggc ccaccgaccg caagatggtg
     5881 atcaacgcgc tcaacagcgg cgccagttgc ttcatggcgg acctcgagga cgcgaactct
     5941 ccaacgtggt cgaacatggt cggcggccag gtgaacctcg cggacgccgt ccgacgcaca
     6001 atcgagctca ccgggccgga aggacgcgag taccggctga cggagcatcc ggcgacgctg
     6061 ctcgtccgtc cccgcggctg gcacctgccc gaacgtcaca tggtcgtcga cggggcactc
     6121 gtgtcgggga gcttgttcga cttcgggctc gcgttcttcc acaacgcgcg cgaacagctc
     6181 gagcgcggca ccgggccgta cttctacctg cccaagctcg agggccatct cgaggcgcgc
     6241 ctgtggaacg acgtcttcct ctgggcgcag gaccgcctgg cgatcgcgcg cggcacgatc
     6301 cgcgcgaccg tcctgatcga gacgatcctg gccgcgttcg agatggacga gatcctgcac
     6361 gagctgcgcg accactcggc gggtctgaac gccgggcgct gggactacat cttcagcgtc
     6421 atcaagaagt tccgtcgcca tccggacttc gtcctgcccg accgagctcg gatctcgatg
     6481 accgtcccgt tcatgcgcgc gtacaccgag ctgttggtgc gcacgtgtca ccggcgcggc
     6541 gcccacgcga tgggcgggat ggccgcctac atccccagcc gccgcgaccc cgaggcgaac
     6601 gagctcgcgc tcgccaaggt cgccgaggac aaggtccgcg aggcgaacga cgggttcgac
     6661 ggcacgtggg tggcccaccc ggacctggtc gggaccgcgg cggccgagtt cgaccgggtc
     6721 ctcggcgatc gcccgaacca ggtcgaccgg cagcgggacg acgtgggggt ctccgcggac
     6781 cagctgctgg acgtgcgcgt cccgggcggc gagatcaccc aggcgggtct gcgcgcgaac
     6841 gtgaacgtcg ggctgaggta cctcatgtca tggctctcgg gcgtgggcgc cgcggcgatc
     6901 gacaacctga tggaggacgc ggcgacggcc gagatctcgc gctcgcagat ctggcagtgg
     6961 gtcgagcacg gcgccgagct ctcgacgggt cagccggtca cggccgaggt ggcccacgcc
     7021 gcgatcacga cagagctcga ggacctgcgc gagcggctcg ggagcgccga gttcgacgag
     7081 cccaggttcg agcaggcgcg gacggtgttc gaacgggtgg cgctctcgcc ggacttcgtg
     7141 gagttcctga cgctgccggc ctacgagctg ctctgatgcc tcatggttca aaccggtcgc
     7201 gatggggaaa ggcgcccgtt cgaggtcgcg ctacccatac gcgaccggaa agggggagcc
     7261 atgaggcgag cgatcatcgt cgcgttgctg gccgcgctgg cgctgggtgt ggtcgccact
     7321 tcggcgccgg cgcagccgtc cagctttcgg gcggtgctct ccggcgccgg cgaggtcccg
     7381 gctcgggaca cccccgccag gggggtcgcc atcttcaact tgaacgctga cgggaccgag
     7441 ctggggttcc aactggtcgt ggcgaacatc gagaacgtct tcgccgcgca catccactgc
     7501 ggggtcgccg gcgtgagcgg tccgatcggg gtcaccctgt tcatgggtgc cccgggtgga
     7561 gggcgtaccg acggcgttct cgccgccggc agcgtgacgg ctcccgacgc cggcaacgca
     7621 tgcggctggg caaccctcgc cgatgtcgtc gccgcggtcg aggcggggaa cgcatatgtc
     7681 aacgcgcaca cgaacgacgg ggtcgcaccc ccgaacaccg gacccggcga cttccctgga
     7741 ggagagatcc gaggtcaggt cttctgagga ggacctagaa ccgttcggcg atcaggtcgg
     7801 cgcaccggac gtcccacccg acgtcggtga agccggtcga aaggccccac agggcgtcgt
     7861 cgagcgagcg gacgaacgcc cagcggcgga cgtgggtcgg ctcaagctcc agctcgttcg
     7921 cgagccggcc cacgatcgcg tcgacggagc cgaggtcgag tcgctccggc agcagcgtcc
     7981 gcacgaggtg agccgtgtcg aacgctcgct cgcccacgag cggcttggga tcgatcacga
     8041 gccagggttc gcgctcggcg gcgagcacgt tccacagatg gaagtcccgg ttggcgagga
     8101 cggcctcctc ggacgagccc gcgaggtcgc ggcagagatc gaccgccgtt cggaggagcc
     8161 gttcgtcgaa gggccgaccc tgccgttcga accgcccggg gagctcgtcg gccaaccgcg
     8221 acgccagatc gcggacgagc gagaacgggt gatcgccagg aaccggccgc cacagacgcc
     8281 ggagcagcga gcaggcgatc ccgatcgcct cggaacgatc ggggtggtcc gcgagcgggg
     8341 tccctggctc cagccgttcg agcaggagcg cgccgcgagc gggttcggcg tccaccagcc
     8401 gcacggcgcc gtcgccgtcc caatgccgca gggcgtcggg ttcgtgctgc gtctcctcgt
     8461 caacgaagct gaccttgagc acgagacgtt cgccgccgcg acgctcggcc ggcgcgacga
     8521 accccacggc tccgcccgcg aacgcgggac cgaccgtcag cgaccatcga ccggcgagct
     8581 cctcgaccag accggggagc gatccaagcc aggctcggcc ggccgccccg ttcgcggaca
     8641 ggttcatccg aaccacctcg tcggggatct cgagcggggt cacggagtgg acggtggtgc
     8701 gccgaggcgg ccgtcggcca tctcgagtac ccggtccagc cgcggcgcga gccccacgtt
     8761 gtgcgtcgcg atcaggcagc tcccgccctc ggctgccgcg cggccgaacg cctcgagcac
     8821 gcgccgctcc gaaacggcgt cctggtgccc cgtcggctcg tccgcgagaa gcagggtcgg
     8881 cgacagcacg agcgcgcggg cgaccgcggt gcgttgctgc tcgccgaggg aggtctcctt
     8941 cgggtagcgc acggccaggt gcgcgatccc cagatcctcc atcagctcgt ccactcgagc
     9001 gtcgagctcc tcgaggcgac cgtggaggcg ggcggggtac tcgaggttct cgcggacgct
     9061 gagctcgtcg aagagcccga gcttctgcgg gaccaccgcg agctcgtccc agcccggggt
     9121 cgcggatcgc ccgccgctcc gctggatcgt tccggcgtcc gcgtgctccc agccggcgat
     9181 cacgttgagc aacgtcgtct tgcccgaacc cgaccgcccg accagaccga cgagctcgcc
     9241 cgcgcggagc gtgaggctca cgtcgtcgag cgcgtggacg acctcgtccc cgcgccggta
     9301 ggccttcgag agcccgttcg cgtccaggac gaacgggcgc tcccgctcgg gggcggtggt
     9361 ggtcatcggc ggccgccatc ggtcggcgcg gtcgggttcg gccgctgccg gggcccggcg
     9421 gaacgcggcg gcgctcgaac gatcggccgg ttcggcgggg gcggtcgtcg acgaccggcg
     9481 catcccgtgg tcgagctcga gcgcggcatc ggcccgccgc atcaccgacc ggtcgtgggt
     9541 cgcgacgacg aacgtcaccc cggcgcgcgc gagctcgacg atcgtgtcca tcacgcggct
     9601 cgcggaggtg ctgtcgagct cggccgtcgg ctcgtccgcc accacgatcg tcgcgccgcc
     9661 catcaggacc tcggcgatcg ccgcccgctg ctgctcgcct cccgagagct ccgagggcag
     9721 gtggtcgaca cgctcctcga tcccgagggt cgagagcagc gcctcctggt cgatccgggg
     9781 cgggcggtgg gtgagcccgc gcgcgagccg aaggtgctcg ccaacggtga ggtgggggag
     9841 gaagttgtcc gacggccgtt ggaacaggta gccgacggtc gcccggcgga accgtcggag
     9901 cgtgcgcacg gaggcgtcgt gcacgctggt cccctcgacc atcagggtgc cgctcgtcgg
     9961 ccggtcgagg cccgcgatca ggcggagcag cgaggactta ccactgcccg acggcccgac
    10021 cacagcgagg agcgaggatg cggggacctc cgcggagagc cccttcagcg cgcgcacctc
    10081 gccgctcgcg gtccggtacg tcttcacgag ctcgtcacag cgcgcggcca cctccggctt
    10141 cctgccagtc tcgggcgcct cagtccgcaa gccgcatcac ctggccgagg tcggcggaac
    10201 gggcacgccg ttcggtgagc catccaccgg cgagcgctac gagcagcagg gccggcgccg
    10261 tgagcgcgac gagggcgacc gggacgaccg tgatcgggtc cggcgggatc gactcgatcg
    10321 ggtccaggag tgggaccgtg agccgtgccg cgaggatcgc gaggacggtg ccggccacgt
    10381 aggcgatcac gagcatcgct ccgacctcca tcgcgatcgc gacgaggtgg cccgaggacc
    10441 gcatccccat ccgcaacgac agcccgtacg agacgatctc ggcgcgctgg cgggcctgga
    10501 ggtacatgag catcgccgca accacgagca gcgccgccag gaccccgagc ccgttcatca
    10561 cgaggaaggt gtcgatcacg gcggcgatgt acgagatgtc cttcacctcg tccgccgtca
    10621 ggacgagcga gggggtgtag tcgagctcgg ccagcgcggc gcgcgccgcg cgaggggtcc
    10681 cgcggatcca gagctcgttg ctcgcgctcg ggccggatag ggggtcgggt gcgtccccga
    10741 acgcgtcgag gaggcgctgc gcgtcgacca cgacgagcgg ccggatcgac gtcatgccgg
    10801 ggaacgcggt cgcgcgcccg acgatctcga tcggtagtgt ctgccggtcg agcgtgatcg
    10861 agtcagggaa ggcgttcggc gccgaggcca tgagcaccgg gagcccgccc ccggacggct
    10921 tcgagatccg gtccaggagc gtctcgagcg gctcggggga gaagccgggg tcccagaacg
    10981 cggtcgaggc gaacgtcttc gggtcgatcg ccagcaggtc gaacgggacg ccgggccgga
    11041 gctcgcccgc ttggaggcgg cggaccaccc gggtgaccgg cagcgggaac gtctccggcg
    11101 tcgcgttcac gctgtcgatc cgagcctcca cgtcgcttcc gacgtacaca cccgccttgg
    11161 cgtcgaccgt ggtccgcatc gagcgcgcca tggtctgcgc ctgcacgaac agcccgaggc
    11221 agagccccgc ggccgcgatg aggagcaacg tcagccgcga gcgcgccgcg agccggtgca
    11281 ccgcgaggta ggcgggggtg ccccagctgc cgctccggcc gcgcgcccac cgaaggacca
    11341 agaccatcgc gcgcgccacc agcgcgacga agcccgcgag gaacacgagc gggaacgcga
    11401 acagcagcag gctcggcgtc tccgtcccga gcgagcgatc gaccacgacc gcgcccccgc
    11461 tccgcagccg ttgcaggatc agcagcgaga ggccgatcag gaccagctcc cacgggaagg
    11521 cacccacga
//
LOCUS       DATBRK010000414         3733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_811817, whole genome shotgun sequence.
ACCESSION   DATBRK010000414 DATBRK010000000
VERSION     DATBRK010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811817"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /gene="eno"
                     /locus_tag="VIH70_12270"
     CDS             <1..172
                     /gene="eno"
                     /locus_tag="VIH70_12270"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010643206.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY8117184.1"
                     /translation="TTIADIAVATNAGQIKAGAPTRGERTAKYNRLLRIEEELGEQAR
                     YPGGAAIRRGAT"
     gene            298..546
                     /locus_tag="VIH70_12275"
     CDS             298..546
                     /locus_tag="VIH70_12275"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY8117185.1"
                     /translation="MAVLAVMAIVPIGRYLDQRSAIVDIERRAAELDAENADLRVEIS
                     RLHDPAELEKLARACLGMVGPGEVAFVIPGRTADRFAC"
     gene            complement(629..2689)
                     /locus_tag="VIH70_12280"
     CDS             complement(629..2689)
                     /locus_tag="VIH70_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117186.1"
                     /translation="MRRGLLLSAVLAIALSSVAVAPVAHAAFPGQNGRIAYVTMAGDH
                     RVIATVDGQGGDPQPLIDLGSGRDAINPVWSKDGLTIAFAGQESPGGKFVIYVANADG
                     TGTPQQITSDYGEPGIWSSDTDPTWSPDGKQIAFVRTLGDGTSTRSAIWRVELATGST
                     TPIYFGSEFDQAEPAWSPDGTRIAFTGKLTGCAQDPCRWGILIWDLERGQFTNFLRLP
                     FGGDGYDWHHPDWSPDGTLIVASFGYDEVPIMDGMSLQLFDVSSGYPVDSWWPCHLTS
                     EPSFSPDGQWLLLTATPVNNETGEREDPILCALRMDRTSGGPLEGTPPRSDAAWGIVP
                     GSSPPPPPPPPPTDTSAPTIEFRPDPSDSEWLDPAWSGSVSIVATDDQSLPSVACTDN
                     GSELSLISGQVGSGTKAVATLADGVHDLVCTATDAAGNSTTASATYRVDLTPPEIVGP
                     SIAPMVARVGDTVSLSANVTDEGSGLQGVRFEAVGSRGDVLDDGPMLESGSGFSFAES
                     LVPVGPDLSQVVVSAVDGLGLIGESSAPFVTYDPLAGSVDGTGWIVPGGPTSEPGDVL
                     PRLDGVQKASFSFTAKYKTPSSVAPAGTLTFSYGSRFKLQSRELLWLAVRDEGTAFLG
                     GNASIQGMDGEYPFVAVFFDGAGGFSGDQFELRVYEPGTEIASPTPLFAASGDVGGQI
                     QIRS"
     gene            3095..>3733
                     /locus_tag="VIH70_12285"
     CDS             3095..>3733
                     /locus_tag="VIH70_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117187.1"
                     /translation="MRLSLSRLTAPLALFALLAGACSSSSAGGSSSPTSSPLSASSLA
                     PVHGTYLPSVDPANFVKTIDNPYWPLQPGTTYTYEGVRGSTPQTDVEVVTHQTKVILG
                     ITSTVVRDTVSEHGAPVERTFDWYAQDKQGNVWYMGEDSFELKGGRFVKASDSWESGV
                     DGAQPGIIMPANPQPGEAYRQEYYPPGEALDEARVLDLNGSVRVPYGSFNALL"
BASE COUNT          628 a         1250 c         1248 g          607 t
ORIGIN      
        1 cacgacgatc gccgacatcg ccgtggcgac caacgccggg cagatcaaag cgggcgcgcc
       61 cacgcggggc gagcggaccg ccaagtacaa ccggttgctg cggatcgagg aggagctcgg
      121 cgagcaggcg cgctacccgg gaggcgctgc gatccggcgg ggtgccacgt gagcccatcg
      181 cccggacgcg gtcccggcac gagtgcgacg aggacgcgga ggcggccgtc gccacgccca
      241 cggctcgagc gcgatcggcc gaggctcacg ggccgcgcgg ccgccctgat cgtcgccgtg
      301 gcggtcctgg ccgtgatggc catcgtcccg atcgggcggt acctcgacca gcgatcggcc
      361 atcgtcgaca tcgagcgtcg agccgcggag ctcgacgccg agaacgccga tctgcgtgtc
      421 gagatctcgc gactgcacga ccccgcggag ctcgagaagc tcgcgcgggc gtgcctgggg
      481 atggtgggcc cgggcgaggt cgcgttcgtc atacccggtc ggaccgccga tcgattcgcc
      541 tgctgatcgc gaatggcagt cgtgctcgac cttcgtagct gtcaggcgga gctcggtcca
      601 accaccagaa acagccagtt acctaccgct agctcctgat ctgaatctgc ccgccaacgt
      661 ccccggatgc cgcgaagagc ggtgtcggtg aggcgatctc cgtcccgggt tcatagactc
      721 ggagctcgaa ctgatcgccg ctgaatccac cagctccgtc aaagaacacg gccacgaacg
      781 gatactcccc gtccatcccc tggatcgagg cgttgccacc aagaaaggcc gtgccttcgt
      841 cccgaacggc gagccacaac agctccctgc tctggagctt gaaccggctg ccgtaggaga
      901 acgtgagggt ccccgccggg gcaaccgatg acggcgtctt gtacttggcg gtgaagctga
      961 acgacgcctt ttggacccca tcgagtcggg gcaggacgtc gcccggctca gaggtaggcc
     1021 cgccgggcac gatccatccc gtaccgtcga ccgagcccgc caacgggtca taggtcacga
     1081 agggagcgga tgactccccg attagcccga ggccgtcgac ggcggatacc acgacttgcg
     1141 aaaggtcggg acctacgggc accaaggact ccgcgaagga gaatcccgac cccgactcca
     1201 acatcgggcc gtcatccagg acgtcgccac gggatcccac cgcttcgaag cggacgccct
     1261 gtaggccgga gccctcatcg gtcacgttcg cggagaggga gacggtgtct ccgactctgg
     1321 ccaccatcgg cgcgatcgaa ggtccgacga tctcgggcgg ggttagatcg acccggtagg
     1381 ttgccgatgc tgtcgtcgag ttgcccgccg cgtccgttgc tgtgcacacc aagtcgtgga
     1441 cgccgtcggc cagcgtcgct acggccttcg tacccgatcc cacctggccg ctgatgagac
     1501 tcagttcgga cccgttgtcc gtgcaggcga cggacggaag ggactggtcg tcggtcgcga
     1561 cgatcgatac cgaccccgac caagcaggat ccagccattc actgtccgaa gggtcgggcc
     1621 ggaactcgat ggttggtgca gacgtgtccg tcggtggcgg tggcggtggc ggtggcgggg
     1681 aggagccggg gacgatgccc catgcggcgt cgctccgcgg tggggttccc tcgagcggac
     1741 ccccgctcgt ccggtccatc cgaagcgcgc agaggatcgg gtcctcgcgc tcgccggtct
     1801 cattgttcac cggcgtcgcc gtgagcagaa gccattggcc gtccggcgag aaggagggct
     1861 cgctcgtaag gtggcacggc caccaactat cgaccggata tccggagctc acgtcgaaca
     1921 gttggagcga catcccgtcc atgatcggga cctcgtcata gccgaacgaa gccacgatca
     1981 gcgttccgtc cggagaccag tccggatggt gccagtcgta gccgtcgccg ccgaacggca
     2041 acctcaggaa gttggtgaac tgacctcgtt cgagatccca gatgaggatg ccccaccggc
     2101 acgggtcctg cgcgcaaccg gtgagcttcc cggtgaaggc gatcctcgtt ccatccggtg
     2161 accacgctgg ctcggcctgg tcgaattcgc tgccgaaata gatcggcgtc gtggagccgg
     2221 tcgccagctc gaccctccag atggccgagc gggtcgaggt cccgtcaccg agcgtgcgga
     2281 cgaacgcgat ctgcttgccg tcgggggacc acgtcggatc ggtatccgag ctccagatgc
     2341 ccggctcccc atagtcgctc gtgatctgtt gtggcgtgcc cgtcccatcc gcgttcgcga
     2401 catagatgac gaactttcct ccaggcgact cttgcccggc gaacgcgatc gtgaggccat
     2461 ccttcgacca cacggggttg attgcatccc gaccactgcc gaggtcgatc aacggctgag
     2521 ggtccccacc ctgcccgtcc accgtggcga tcacgcgatg gtcgcccgcc atcgtcacgt
     2581 aggcgatccg cccgttttgc ccggggaacg cggcgtgcgc gaccggcgcg accgccacgc
     2641 tcgagagtgc gatggccaac accgcgctca acaacagacc tcgtctcatg gggaaggctc
     2701 ccgtggcagg gtcccccaga gtaggagcgg gaaagccagc cggacaatcg gtcaaacggc
     2761 gactgggtct cttacgacct ttggccgtgt gtaggtagct ctcgcccttg cgcgccacga
     2821 cctgtgttcg gatgtgacga acctgtgaac agatctctgc gtccgagccc tagccgagcc
     2881 agcggctaat gcccgtagga ggtggtgatc atgatcggat cgctcgcggc ctacggggat
     2941 ggagggggag gctccggcgg tgcctattgg gtggtcgttg tcgtcgcggc ggcggtcgtc
     3001 atcgccgcgg gctcatggct gttcgttcgc atgcggcgtc gtgcatccga tcgaacggac
     3061 acgagtcctc gttcacctca gccgaggtga ggccgtgcgc ctctccctgt cgaggctgac
     3121 ggcgccgctg gcgctcttcg cccttctcgc cggcgcgtgc agctcgtcga gtgcaggggg
     3181 ctcgtcgtca ccgacctcct ccccgctgtc cgcgtccagt ctggcaccgg tccacgggac
     3241 ctacctaccg tcggtcgatc ccgcgaactt cgtcaagacg atcgacaatc cctactggcc
     3301 gctccagccg ggaacgacct acacctatga gggcgttcgc ggctccacgc cgcagaccga
     3361 cgtcgaggtc gtgacgcacc agacgaaggt gatcctcggc atcacgtcca cggtcgttcg
     3421 ggacacggtc tccgagcacg gtgctccggt ggaacggacc ttcgattggt acgcccagga
     3481 caagcagggc aacgtctggt acatgggtga ggactccttc gagctgaagg gcggtcgttt
     3541 cgtcaaggcc agcgactcat gggagtcggg ggtcgacggc gcgcagccgg ggatcatcat
     3601 gccggccaac ccgcagccgg gcgaagccta tcggcaggag tactacccgc cgggcgaggc
     3661 gctcgacgag gcgcgtgtcc tcgacctgaa cggaagcgtc cgggtgccgt acgggagctt
     3721 caatgcgttg ctc
//
LOCUS       DATBRK010000415         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2402693, whole genome shotgun sequence.
ACCESSION   DATBRK010000415 DATBRK010000000
VERSION     DATBRK010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402693"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VIH70_12290"
     CDS             <1..843
                     /locus_tag="VIH70_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY8117188.1"
                     /translation="ITAAAAIQERAIPLGTQLTVPWTMPVGDYIIHDSQRHAPMRMTL
                     GDIVAESSNIGAVMVADRLGESALATYLARFGLGRATGVGFPGESNGIMLPLDQWNDT
                     NLATMAYGQGIAATPLQMVSVYSTIANDGRWVQPKLVRGTIGPDGSLEPNDAAPTRRV
                     VSAETARMVTRMLAYAVQDGTGTNAQIPGYQVAGKTGTARIPTGDGSGYLDGQYISSF
                     IGYLPAGDPEVVVAAILDRPAAVYGGLAAAPLFKQVALAAIARLDIAPAHRVPLPPHA
                     LPVG"
     gene            894..2387
                     /locus_tag="VIH70_12295"
     CDS             894..2387
                     /locus_tag="VIH70_12295"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY8117189.1"
                     /translation="MAPPHSRTLDDLVRELPEAELRGSADVLVSSVCYRSDDARPGAL
                     FFCVPGSTSDGHAFAGEAVSRGSTGIVVDRWLDLDVSQVKVPVVREAMGPISAEFYGR
                     PADGMTVVGVTGTNGKTTTTFLLEAILRADGRTPGVIGTTGVRIDGRPEPFPRTTPEA
                     PDLQGLVATMADEGVTAVAMEVSSHGLHQHRVDGIRYSVAVFTNLTQDHLDYHPSMEE
                     YFLAKSRLFTPLMSDRAVVNLDDERGRDLAGSGLPTITYGLAPESDVRATDVESTAEG
                     IAFLADGVPISSALRGSFNVQNALAAFATARSLGIEDRVAADAISSVRVVPGRVESVE
                     VGQGFLVLVDYAHTPDSVANVLRAARSLTDGRLIVVLGAGGDRDRVKRPLMGRAATAN
                     ADLAVITSDNPRSEDPLDIIAEITPGAERGAEEGGGRFVVEPDRRTAIRLAMQEASPG
                     DVVVVAGKGHETYQELADRRIPFDDRAVAEDELRSIGKAARPLETAP"
BASE COUNT          361 a          869 c          862 g          346 t
ORIGIN      
        1 atcacggccg cggccgcgat ccaggagcgg gcgatccccc tcggcacgca gctcacggtc
       61 ccgtggacga tgccggtcgg cgactacatc atccacgact cacagcggca cgcaccgatg
      121 cggatgaccc tcggggacat cgtggccgag tccagcaaca tcggcgcggt catggtcgcg
      181 gaccgtctcg gcgagtccgc cctcgcgacc tacctcgcgc ggttcgggct cgggcgcgca
      241 acgggcgtcg ggttcccggg ggagtccaac ggcatcatgc tcccgctcga tcagtggaac
      301 gacaccaacc ttgccacgat ggcgtacggc caggggatcg cggcaacgcc gctccagatg
      361 gtctcggtgt actcgacgat cgcgaacgac ggtcgctggg tgcagcccaa gctggtgcgc
      421 gggacgatcg gccccgacgg gtcgctcgaa ccgaacgatg ccgctcccac gaggagggtc
      481 gtctcggccg agacggcgag gatggtcacg cggatgctcg cctatgcggt gcaggacggc
      541 accggcacga acgcgcagat ccccgggtac caggtcgccg gcaagaccgg aacggcgagg
      601 atcccgaccg gcgacggctc cggctacctc gatggccagt acatctcgtc gttcatcggc
      661 tacctcccgg cgggggaccc ggaggttgtc gtcgcagcga tcctggatcg tccggcggcc
      721 gtgtacggcg ggctcgccgc ggcgccgctc ttcaagcagg tcgccctcgc cgcgatcgcc
      781 cgcctcgata tcgcgcccgc ccaccgggtg cccctgccgc cgcacgccct gccggtcgga
      841 tgatcgcccg cacgcgggag ggcgccgtca cgcaggcgat aggctcagga accgtggccc
      901 ctccccactc ccgaacgctc gatgacctcg tccgggaact ccccgaggcc gagctccgag
      961 gcagcgcgga cgtgctcgtc tcgagcgtct gctaccgctc ggacgacgcc cgcccaggcg
     1021 ccctgttctt ctgcgtcccc gggagcacgt ccgacggtca tgccttcgcg ggcgaagcgg
     1081 tctcgcgcgg gtcgacgggg atcgtcgtcg accgctggct cgatctcgac gtgtcgcagg
     1141 tgaaggtgcc ggtcgtgcgc gaggcgatgg gtccgatctc ggccgagttc tacgggcggc
     1201 cggcggatgg gatgaccgtc gtcggcgtga ccggaacgaa cgggaagacc acgacgacct
     1261 tcctgctcga ggcgatcctt cgggcggacg gccggacccc gggcgtgatc ggtaccaccg
     1321 gtgtgcggat cgacggccgg cccgagccct tcccccgaac gacccccgag gcgccggacc
     1381 tgcagggact cgtggccacg atggccgacg agggcgtcac cgccgtcgcg atggaggtct
     1441 cctcgcacgg cctgcatcag caccgcgtcg acggcatccg gtactccgtc gcggtgttca
     1501 ccaacctcac acaggatcac ctggattatc acccgtcgat ggaggagtac ttcctcgcga
     1561 agtcgcggct gttcacgccg ttgatgagcg atcgcgccgt cgtgaacctc gacgacgagc
     1621 ggggccgcga tctggcaggc tcgggtctgc cgacgatcac gtacgggctc gcgccggagt
     1681 cggacgtgcg agccacggat gtggagtcga cggccgaggg gatcgccttc ctcgccgacg
     1741 gcgtcccgat cagctcggcc ctgcgcgggt cgttcaacgt ccagaacgct ctggccgcct
     1801 tcgccaccgc gcgatcgctc gggatcgagg accgcgtcgc ggccgacgcg atctcatcgg
     1861 tgcgcgtcgt ccccggacgg gtggagtcgg tcgaggtggg gcaggggttc ctcgtgttgg
     1921 tcgactacgc ccatacgccc gacagcgtgg cgaacgtgtt gcgcgcggcc cggtcgctca
     1981 cggacggccg gctgatcgtg gtgctcgggg ccggcggcga tcgcgaccgt gtcaagcgac
     2041 cgctcatggg ccgagcggcc acggccaacg ccgacctcgc ggtcatcacg agcgacaacc
     2101 cccgcagcga ggacccgctc gacatcatcg ccgagatcac gccgggcgcc gaacgcgggg
     2161 ccgaggaggg aggtggccgt ttcgtcgtcg agccggatcg gcgcaccgcg atccggctcg
     2221 cgatgcagga ggcctcaccc ggcgacgtcg tcgtggtcgc cggcaaggga cacgagacct
     2281 accaagagct cgcggaccgg aggatcccct tcgacgaccg agcggtcgcc gaggacgagc
     2341 tgcgatcgat cgggaaggcc gcccgtccgc tggagacggc gccgtgatcg agcgtcggct
     2401 ctcggagatc gcgtccgccg tccgcggcac gctccgcg
//
LOCUS       DATBRK010000416         3271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_94834, whole genome shotgun sequence.
ACCESSION   DATBRK010000416 DATBRK010000000
VERSION     DATBRK010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94834"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..396
                     /locus_tag="VIH70_12300"
     CDS             103..396
                     /locus_tag="VIH70_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY8117190.1"
                     /translation="MTAAENALLSLLCIVLTVYTILLFVRVIISWSYLFGFRPPYSGP
                     LRTILDLLDDVTEPVLRPLRALIPPIRAGGVGLDLSILIAFVILAVLRIALNC"
     gene            414..1238
                     /locus_tag="VIH70_12305"
     CDS             414..1238
                     /locus_tag="VIH70_12305"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEY8117191.1"
                     /translation="MRRKKGTDDAPATGARITPEEVQQVEFRLAFRGYNERDVDAFLD
                     RITEGLSSYLEELERLRLAVGQPSRSFAGSTGDAAAMVARARAEADEIVRRAHEQAAG
                     IRAAGGSGPGDARGAVAPFLNREREFLQSLGGLVQGHAEEIKQMVVAIRERAEAPSVP
                     DTARSVATPTSVPGVGPSAEAGAAGERASASSPSQVSPAAPPEDLEPASAAEIRERLR
                     ASEPPVEIGPADDDNLPSQASPIVVESGTEPFFSTEGGPATERRERSLRELFWGED"
     gene            complement(1291..>3271)
                     /locus_tag="VIH70_12310"
     CDS             complement(1291..>3271)
                     /locus_tag="VIH70_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY8117192.1"
                     /translation="CDTPLIYIARGSWYVGTTAVKDRLLAVNEEVDWYPSHIKHGRYG
                     DWLENNVDWALSRERYWGTPLPIWRCDQEHDTAIGSLTELSELAGRDVTGIDPHRPAI
                     DEVSVACPSCGGDSTRVPEVIDTWYDSGAMPYAQWGYNPELGRGLERFRERFPADFIA
                     EAIDQTRGWFYTLMAEGVLLFDATTYSTVVCLGHLVDRDGRKMSKSLGNTVDAFEVMD
                     RQGADALRWFLLTGGSPWSSRRVGMEMFDDVVRQFLLPLWNVYAFFVTYANASGFDPG
                     EGYDVPVAQRPLLDRWVLSQLSRTETAAREGLEEYDATGAGRRIADFVDDLSNWYVRR
                     ARRRFWDPAGQGGRDVRAAFLTLHACLVSLSRLLAPFTPFVAETLWRNLAAGRDGAPD
                     SVHLSDYPTADERLVDPTLDEAMAAVRQIVELGRRVRTETKVRVRQPLLEAVVHYAGD
                     HAALEPLLDLVGEELNVKQVLFAETAAQLGRWRAKPNFKVLGPRLGRGVKDVAAALEW
                     DDGSLAAALAHGDPVWVDLGSEQVELAPSDVDLVQETVEGWGVASDGGITVALELELT
                     PELRAEGLARELVRAIQDVRKTAELDVGDRIVLGLQIEGPTAEALDAAIGAHRAWMGA
                     ETLAVEIVDGTAPDATFEQTFEIEDARVRVSLRRA"
BASE COUNT          459 a         1205 c         1130 g          477 t
ORIGIN      
        1 cgcccgcgaa cgtggacgtc tccgccgagg accgcgagcg catcgccggc ggcgacctct
       61 acaaccagtt ctgacgccgc tcgatcgttc gacccgtccg cgatgaccgc cgccgagaac
      121 gcgctgctca gcctcctgtg catcgtactc acggtgtaca cgatcctgct gttcgtccgc
      181 gtgatcatct cgtggtcgta cttgttcggg ttccgcccgc cgtactccgg gccgcttcgg
      241 acgatcctgg atctgctcga cgacgtcacc gagccggtcc tgcgccccct ccgggcgctg
      301 atcccgccga tccgggcggg cggcgtcggg ctcgacctct cgatcctcat cgcgttcgtt
      361 atccttgcgg tcctgcggat cgcgctgaac tgctgacgga gggggacggc tgcatgcgga
      421 ggaagaaggg aacggacgac gccccggcga ccggcgcccg catcacgccg gaggaggtgc
      481 agcaggtgga gttccgcctg gctttccggg ggtacaacga gcgcgacgtc gacgcgttcc
      541 tggaccggat caccgagggc ctgtcctcct acctcgagga gctcgagcgc ctgcggctgg
      601 ccgttggaca gccgtcccgg tccttcgcgg gctccaccgg cgacgccgcc gccatggtgg
      661 cccgggcgcg cgccgaggcc gacgagatcg ttcggcgtgc ccacgagcag gccgccggga
      721 tccgggccgc gggcggctcc ggcccgggcg acgctcgggg ggccgtggcc ccgttcctga
      781 accgtgaacg ggagttcctc cagagcctcg gtggtctcgt ccaggggcac gcggaagaga
      841 tcaagcagat ggtggtcgcg atccgcgagc gggccgaggc gccctcggtg cccgacaccg
      901 cacgttccgt cgccacgccg acgtcggtgc cgggggtcgg tccctcggcc gaggcgggcg
      961 ccgcgggcga acgagcatcg gcctccagcc cttcccaggt gtccccggcg gcaccccccg
     1021 aggacctcga gccggcctcg gccgccgaga tccgggagcg gctccgggcg agcgagccgc
     1081 cggtcgagat cgggccggcg gacgacgaca acctcccgtc gcaggcgtcg ccgatcgtcg
     1141 tcgagtccgg gaccgagccc ttcttctcga cggagggggg gcccgcgacc gagcgacgcg
     1201 aacgctcgct gcgcgagctc ttctggggcg aggactaagc cgaacgcccg gcctcggctc
     1261 gagctctgag ccgagcttct cggctcccgc tcacgcccgc cggaggctga ccctcaccct
     1321 ggcgtcctcg atctcgaagg tctgctcgaa cgtcgcatcg ggcgccgtcc cgtcgacgat
     1381 ctccacggcg agggtctccg cgcccatcca cgctcggtgc gcgccgatcg ccgcgtcgag
     1441 cgcctcggcg gtcggaccct cgatttggag gccgagcacg atccgatcgc cgacgtcgag
     1501 ctcggcggtc ttgcgcacgt cttggatcgc tcgcaccagc tcgcgggcga ggccctcggc
     1561 ccggagctcg ggcgtcagct cgagctcgag cgcgacggtg atgccgccgt cgctcgcaac
     1621 cccccaaccc tcgacggtct cctggacgag gtcgacgtcc gagggcgcga gctcgacctg
     1681 ttcggacccc aggtcgaccc acacgggatc gccgtgggcg agcgcggcgg ccagcgaccc
     1741 gtcgtcccac tccagggccg ccgccacgtc cttgacccca cgtccaagcc ggggcccgag
     1801 gaccttgaag ttcggcttgg ctcgccaccg gccgagttgc gcggcggtct ccgcgaacag
     1861 cacctgcttc acgttcagct cctcgccgac cagatcgagc aacggctcca gggccgcgtg
     1921 gtccccggcg tagtgcacga cggcctcgag caacggctgc cgcacgcgca cctttgtctc
     1981 ggtccggacg cggcggccga gctcgacgat ctggcggacc gcggccatag cctcgtcgag
     2041 cgtcggatcc accagtcgct cgtcggcggt cgggtagtcc gacagatgca ccgagtccgg
     2101 cgcgccgtcg cgcccggcgg ccaggttgcg ccacagggtc tccgcgacga acggtgtgaa
     2161 gggcgcgagc agccgcgaga ggctcacgag gcacgcgtgc agcgtgagga aggccgctcg
     2221 gacgtcgcgg cctccctggc cggcggggtc ccagaatcgc cggcgggcgc gccgcacgta
     2281 ccagttcgac aggtcgtcca cgaagtcggc gatccgccgg ccggcgccgg tcgcgtcgta
     2341 ctcctccagg ccctcgcggg cggcggtctc ggtccgcgag agctgcgaca ggacccatcg
     2401 gtccaggagc ggccgctgcg cgacgggcac gtcatagccc tcgcccgggt cgaacccgct
     2461 cgcgttcgca tacgtgacga agaacgcgta aacgttccac agcgggagca ggaactgccg
     2521 gacgacgtcg tcgaacatct ccatgccgac gcggcgagac gaccacggcg agccccccgt
     2581 gagcaggaac cagcggagcg cgtccgctcc ctgccgatcc atgacctcga acgcgtccac
     2641 ggtgttgccg agcgacttcg acatcttgcg cccgtccctg tcgacgaggt gcccgaggca
     2701 caccacggtg ctgtaggtcg tggcatcgaa caggagcacg ccctcggcca tcagcgtgta
     2761 gaaccaaccg cgggtctggt cgatcgcctc ggcgatgaag tcggcgggga agcgctcgcg
     2821 gaaccgttcg agcccgcgcc ccagctcggg gttgtagccc cactgtgcgt agggcatcgc
     2881 gcccgagtcg taccaggtgt cgatcacctc cgggaccctg gtcgagtcgc caccgcagga
     2941 ggggcacgcg accgacacct cgtcgatcgc cggacgatgc gggtcgatcc cggtcacgtc
     3001 gcggcctgca agctcggaga gttccgtgag cgacccgatc gcggtgtcgt gttcctggtc
     3061 gcatcgccag atcggcagcg gggtgcccca gtagcgctcg cgcgagagcg cccagtccac
     3121 gttgttctcc agccagtcgc cgtagcgccc gtgcttgatg tgcgacggat accagtcgac
     3181 ctcctcgttc accgcgagca gccggtcctt gaccgcggtc gtccccacgt accaggagcc
     3241 cctggcgatg tagatcagcg gcgtgtcgca t
//
LOCUS       DATBRK010000417         6405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2258487, whole genome shotgun sequence.
ACCESSION   DATBRK010000417 DATBRK010000000
VERSION     DATBRK010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258487"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1055)
                     /locus_tag="VIH70_12315"
     CDS             complement(9..1055)
                     /locus_tag="VIH70_12315"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY8117193.1"
                     /translation="MATRAVEAGAAGHGAEPRGKRTDQGEATYLVAIAEALWEEMERD
                     ERVFLLGEDIGVYGGAFKVTEGFIERFGPTRVMDTPIVEETIVGTALGAAMEGLRPVA
                     EFQYADFMSSGYDEITTAMARYHYRSGVPVPVVLRGPSGGGVRASSFHSTNPEPWLSQ
                     APGLKVICPSFPSDAKGLLKAAIRDDNPCVFLEHKWIYRRIKEIVSDDPDHLVPIGKA
                     DVKREGTDVSVIAHGMMVHLALEAAETLANEGIRVEVLDLRTVQPLDVEAILATVTKT
                     SRAVVLQESQRYLGTGAEVASLIAEEAFESLDAPVVRLSPPHVPVPFSPPLEDAYLPQ
                     VSDIEDAVRKLSAW"
     gene            complement(1055..2119)
                     /locus_tag="VIH70_12320"
     CDS             complement(1055..2119)
                     /locus_tag="VIH70_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY8117194.1"
                     /translation="MATKVERQKLAADDWRVGLSDERLRDLLYMLKLCRYFDERIEAL
                     YRQGRLPGAIYSGRGQEGTHVGVATALRKDDSLFPTHRDLSAQLAKGLDLDRVMAQFW
                     GRIDGYTRGRDGNSHVGDWQGNRTWTVMSHLPIAYPVACGAALAYKMRGEPRVALAIC
                     GDGATSNGRWHEALNLSAIHQLPVVWVVNNNQFAYSTPNPLEFPVPTIAERAGAYAMP
                     GFRVDGQDVLDVYRIAHDAVERAREGGGPTLIESVSLRWRGHAGHDPAKYVPKELLDE
                     YMRDKDPVRRFEELVRSRGIVSEQDVTDLEERIRAAFEAGYEHALSSPFPEPGDVTKG
                     LYTDDGYWAAEPGRGGGTES"
     gene            complement(2122..3498)
                     /gene="lpdA"
                     /locus_tag="VIH70_12325"
     CDS             complement(2122..3498)
                     /gene="lpdA"
                     /locus_tag="VIH70_12325"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY8117195.1"
                     /translation="MSDAYDIVVIGSGTGGYSCALRAAQLGKRVAIVERDERLGGTCL
                     LRGCIPTKALLQSAAVMDTVNRSEEWGIKASGEPDWTQVQAFQTKIVDKLVSGLTGLV
                     KARKIDVIRGSAVLGAGPGVQVGDRTITATDVVIATGSRPKLLPGLQVTPRIITSDEA
                     LWHAEIPSSAVIIGAGAIGLEFASFYRSMGADVTLVEALPRVAPLEDEDLSKEIARAF
                     RKRGIAAHAGASVTGVSDTGDHVEVAFEAGGAEQAVTADVCLVAVGRGPVTDELGLED
                     AGVELDRGFVKVDGQLQTTASHVWAIGDVAATPLQLAHVSFTEGYAVAERIAGVPVPE
                     IDYAQIPRVTYCSPEIASVGITEAQAIERGHDVDVERFNFQGVGKANIVGEGGLVKIV
                     AAKEGPVLGVHLVGPHVTDLVSEAMLAVAWEAVPAEVAALIHPHPTLSEGVGEAFLAL
                     AGKPLHGA"
     gene            3560..4669
                     /gene="gcvT"
                     /locus_tag="VIH70_12330"
     CDS             3560..4669
                     /gene="gcvT"
                     /locus_tag="VIH70_12330"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY8117196.1"
                     /translation="MTDTPKPDPETRHGPLEDLHRALGAKLGPFGGWLMPIEYGGVLS
                     EHRAVRERVGLFDLSHLGKVDVVGAPAFEALQGLLTNDLRKVDVGRAQYHHMLNERGG
                     VEEDLFLYRLAGDRWFLVPNAANKDKVVAAVRSRGAEVVDHDGWCFLGVQGPRSHAVV
                     SEIWRSAVELPFRGVLVVDWHGTEVVLARTGYTGERGFELFADAGIAAELWDAIVTAG
                     RPFDIEPCGLGARDVLRLEMGYPLYGQDLGPDRTTLEAGLGWAVSLDKGPFVGREALE
                     RQREQGLPSLLRGVATADRRHIPRAHQPVIAGGRTAGEVTSGTFSPLLGHGIALAYLS
                     PPDAFEPGVAVEIDIRGRAAPATIVTTPFADRSPR"
     gene            complement(4704..5426)
                     /locus_tag="VIH70_12335"
     CDS             complement(4704..5426)
                     /locus_tag="VIH70_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117197.1"
                     /translation="MRNLIGSLVIALAAVACGEAVATPPPGADTSGLLIHQSPSPFQQ
                     VSAGPVSALIPDAWEPRLAGPIDDPRQGIVAGPQPQEWRSDRPPLEGLAAMWIDGTAI
                     GVPSDYYYLAATGPALDVLTGSEQCRATRRFVVARHLPAFAQGAPDSPGDYVAHGLGT
                     CTVGNRPTRWAYFVAAPGYGPLRQVGIPSSGLYVVVAVMPASPRSSFLLNRLLRRTEF
                     NGSSVADMLEAAAPVPTPGDLA"
     gene            complement(5491..6231)
                     /locus_tag="VIH70_12340"
     CDS             complement(5491..6231)
                     /locus_tag="VIH70_12340"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEY8117198.1"
                     /translation="MRERIRPVLAALSFLSAVPVGRAVELGPRDLRRSAALLPVVGGV
                     VGALVAVVAWGSAQVLPTFVAAVLGIAAGVAVTAALHLDGLGDVADGIGASLAGSDPA
                     RAMRDPRLGTFGVAAVVLDLLLKTSTLSALMVTGFPWPVVAAGTLSRVVPVAMAWRLP
                     YAGGGAGTWTAGIGARTVAIASAVALAIAIPSAAIATPGMIVAVVAASILIGSWSNRR
                     LGGVSGDVFGASIELGETFALVAALAAR"
     gene            complement(6224..>6405)
                     /locus_tag="VIH70_12345"
     CDS             complement(6224..>6405)
                     /locus_tag="VIH70_12345"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014318018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEY8117199.1"
                     /translation="GLEPLLDLGLRLGEGTGAALALPLVRSAIAILTDMATFDAAGVD
                     GPVPAVEPRSAAGDA"
BASE COUNT          917 a         2328 c         2275 g          885 t
ORIGIN      
        1 ctccggggtc accatgcgga cagcttgcgg acggcgtcct cgatgtcgga cacctgcggc
       61 aggtatgcgt cctcgagcgg cggagagaac ggcaccggga cgtgaggcgg cgagagccgg
      121 acgaccggtg cgtcgagcga ctcgaacgcc tcctcggcga tcaacgaggc gacctccgcg
      181 cccgtgccga ggtagcgctg gctctcctgg agcaccaccg cacggctcgt cttggtcacc
      241 gtggccagga tcgcctccac gtccagcggt tgaacggtcc gcaggtcgag cacctcgacc
      301 cggatgccct cgttcgccag ggtctcggcc gcctcgagcg cgaggtgcac catcatcccg
      361 tgcgcgatca ccgagacgtc cgtgccctcg cgcttgacgt cggccttgcc gatcgggacg
      421 aggtggtccg ggtcgtccga gacgatctcc ttgatccgac gatagatcca cttgtgctcc
      481 aggaacacgc aggggttgtc gtcgcggatc gccgccttca gcagtccctt ggcgtccgag
      541 gggaacgatg ggcagatgac cttcagaccg ggcgcttgcg agagccacgg ctcggggttc
      601 gtcgagtgga agctcgaggc cctgaccccg ccgcccgagg gcccgcgcag caccaccggg
      661 accggcaccc cgctccgata gtgatagcgg gccatcgcgg tcgtgatctc gtcgtagccg
      721 ctcgacatga agtcggcgta ctggaactcg gcgaccggac gcagaccctc catcgccgcg
      781 ccgagcgcgg tcccgacgat cgtctcctcg acgatcgggg tgtccatcac gcgcgtcggg
      841 ccgaaccgct cgatgaatcc ctcggtcacc ttgaacgcgc cgccgtagac gccgatgtcc
      901 tcgccgagca ggaacacccg ctcgtcccgc tccatctcct cccacagcgc ctcggcgatg
      961 gcgacgagat aggtggcctc gccctggtcg gtccgcttgc cccgcggttc ggcaccgtgg
     1021 ccggcggcac ccgcctcgac ggctcgcgtc gccatcagga ctcggtcccg ccgccccggc
     1081 cgggctcggc ggcccagtag ccgtcgtcgg tgtacaggcc cttggtgacg tcgcccggct
     1141 cggggaacgg tgaggacaac gcgtgctcgt agccggcctc gaacgccgcc cggatccgct
     1201 cctcgaggtc ggtcacgtcc tgctcgctca cgatgccgcg cgaccggacc agctcctcga
     1261 agcgcctcac gggatccttg tcgcgcatgt actcatcgag cagctccttg gggacgtact
     1321 tggccgggtc gtggccggcg tgcccccgcc agcgcagcga gacgctctcg atcagcgtcg
     1381 ggccgccacc ctcgcgggct cgttcgaccg cgtcgtgcgc gatccggtac acgtcgagca
     1441 cgtcctgccc gtcgacgcgg aagcccggca tcgcgtacgc gccggcacgc tcggcgatcg
     1501 tcgggaccgg gaactcgagc gggttgggcg tcgagtacgc gaactggttg ttgttgacga
     1561 cccagacgac cggcagctgg tggatcgccg agaggttcag cgcctcgtgc cagcggccgt
     1621 tcgaggtggc cccgtcgccg cagatcgcca acgcgacccg cggctccccg cgcatcttgt
     1681 aggcgagcgc cgccccgcag gcgaccgggt aggcgatagg aaggtgggac atgaccgtcc
     1741 aggtccggtt cccctgccag tcgcccacgt ggctgttgcc gtcgcggccc cgcgtgtagc
     1801 cgtcgatccg cccccagaac tgcgccatca cgcggtccag gtccaggccc ttggcgagct
     1861 gcgccgagag gtcgcggtgc gtcgggaaca gcgagtcgtc cttgcgcagg gcggtggcga
     1921 ccccgacgtg ggtcccctcc tgaccgcgac ccgagtagat ggcgcccggg aggcgcccct
     1981 gccggtacag ggcctctatc cgctcgtcga agtaccggca gagcttcagc atgtacaaca
     2041 ggtccctgag ccgctcatcc gacagcccga cgcgccagtc gtccgccgcg agcttctgtc
     2101 tctccacctt cgtcgccatg ttcacgctcc gtgcaggggc tttccggcga gcgccaggaa
     2161 ggcctcaccg acgccctcgg acagcgtggg gtgcggatgg atcagggcgg cgacctcggc
     2221 gggaacggcc tcccacgcga ccgcgagcat ggcctcggag acgaggtcgg tgacatgcgg
     2281 ccccaccaag tggaccccga gcaccgggcc ctccttggcg gcgacgatct tgacgagccc
     2341 accctcgccc acgatgttgg ccttgccgac cccctggaag ttgaaccgct cgacgtccac
     2401 gtcgtggccg cgctcgatcg cctgcgcctc ggtgatgccg acggacgcga tctcgggcga
     2461 gcagtaggtc acgcgcggga tctgcgcgta gtcgatctcg ggcaccggca cgccggcgat
     2521 gcgctccgcg acggcgtagc cctcggtgaa cgagacgtgg gcgagctgca gcggggttgc
     2581 cgcgacgtcg cctatcgccc agacgtgcga ggcggtggtc tgcagctggc cgtcgacctt
     2641 gacgaacccg cggtccagct ccaccccggc gtcctcgagc ccgagttcgt cggtgaccgg
     2701 tccccggccg acggcgacca ggcagacgtc ggccgtgacg gcctgctccg cgccacccgc
     2761 ctcgaaggcg acctcgacgt ggtcgcccgt gtccgacacg ccggtcacgc tcgcgccggc
     2821 gtgagccgcg atcccgcgct tacggaacgc gcgcgcgatc tccttcgaca ggtcctcgtc
     2881 ctccagcggc gcgacccgcg ggagcgcctc gaccaacgtt acgtcggcgc ccatcgaacg
     2941 gtagaagctc gcgaactcga gcccgatggc ccccgccccg atgatcacgg ccgacgaggg
     3001 gatctcggcg tgccacagcg cctcgtcgct cgtgatgatc cgaggcgtga cctgcagccc
     3061 cggcaggagc ttggggcgcg acccggtggc gatgaccacg tcggtggcgg tgatcgtccg
     3121 atcgcccacc tggacccccg gccccgcccc caggaccgcg gacccgcgga tcacgtcgat
     3181 cttccgggcc ttcaccagtc cggtcagccc ggatacgagc ttgtcgacga tcttggtctg
     3241 gaacgcctgg acctgcgtcc agtccggctc gccggaggcc ttgatgcccc attcctcgga
     3301 acgattgacg gtgtccatga ccgctgcaga ctgcaggagc gccttggtgg ggatgcagcc
     3361 gcgcagcagg caggtgccgc ccagtcgctc atcgcgctcg acgatcgcga cccgcttgcc
     3421 caactgtgcc gcccgcaggg cgcaggagta gcctcccgtc cccgagccga tcacgacgat
     3481 gtcgtaggcg tcggacatcc gaacggccat gctaccgcgg ttccgtgcga cgactcctcg
     3541 accgtaggat gacgcggccg tgaccgacac acccaagccc gatccggaga cgcggcacgg
     3601 gcccctcgag gatctccacc gagccctggg agccaagctg ggtccgttcg gcgggtggtt
     3661 gatgccgatc gagtacggag gcgtcctgtc cgagcaccgc gcggtccgag agcgcgtcgg
     3721 gctgttcgac ctcagccacc tcggcaaggt cgacgtcgtg ggcgccccgg cgttcgaggc
     3781 gttgcaggga ctgttgacca acgacctgcg gaaggtcgac gtcggccggg cgcagtacca
     3841 ccacatgctg aacgagcgcg ggggtgtcga agaggacctg ttcctttacc gcctcgccgg
     3901 cgatcggtgg ttcctggtcc cgaacgcggc caacaaggac aaggtcgtcg cggcggtgcg
     3961 gtcccggggg gccgaggtcg tcgaccacga cggctggtgc ttcctcggcg tccagggacc
     4021 gaggtcgcac gcggtggtga gcgagatctg gcggagcgcg gtcgagctcc ccttccgcgg
     4081 ggtcttggtc gtcgactggc acgggaccga ggtcgtgctc gcgcggacgg gttacacggg
     4141 agagcgcggg ttcgagctgt tcgccgacgc cgggatcgcc gccgagctct gggacgcgat
     4201 cgtcacggcg gggcggccgt tcgacatcga gccgtgcggg ctcggggcgc gcgacgtgtt
     4261 gcgtctggag atgggctacc cgctctacgg tcaggacctg ggcccggacc ggacgacgct
     4321 cgaggccggg ttggggtggg cggtgtcgct cgacaagggt ccgttcgtgg gacgcgaggc
     4381 gctcgagcgc cagcgcgagc aggggctgcc gagtctcctg cgaggcgtcg cgaccgccga
     4441 ccgccgccac atcccgcgcg cgcaccagcc ggtgatcgcc ggcgggcgca ccgccgggga
     4501 ggtgacgagc gggaccttct cgccgctgct cggtcacggg atcgcgctcg cgtacctctc
     4561 gccgccggac gcgttcgagc cgggcgtcgc cgtcgagatc gacatccgcg gccgcgcggc
     4621 accggcgacc atcgtcacga cgccgttcgc ggatcggtcc ccgcgctgag cgggttcgtc
     4681 ggtgaacgcg cgaccgttgc ggttcaggcc aggtcgccgg gcgtgggaac cggtgcggcc
     4741 gcctcgagca tgtcggccac cgacgacccg ttgaattcgg tgcgccgcag cagccggttg
     4801 agcaggaacg acgagcgggg ggacgcgggc atcacggcga ccacgacgta caggcccgag
     4861 ctggggatcc cgacctggcg caagggcccg tagcccggcg cggccacgaa gtaggcccac
     4921 cgggtcggtc ggttgccgac cgtgcaggtg ccgagaccgt gggccacgta atcgcctggc
     4981 gagtcgggcg ccccctgcgc gaacgccggc aggtgcctgg ccacgacgaa ccggcgggtc
     5041 gcgcggcact gttccgaacc cgtgaggacg tccagcgcgg gtccggtggc cgcgaggtag
     5101 tagtagtccg acggcacgcc gatggcggtc ccgtcgatcc acatcgcggc gagcccctcg
     5161 agcggtggcc ggtcggacct ccactcctgc ggttgcggtc cggcgacgat tccctggcgg
     5221 gggtcgtcga tgggcccggc caggcgcggc tcccacgcat cggggatcag cgcggagacc
     5281 ggtcccgccg acacctgttg gaacgggctc ggggactggt ggatcagcag gcccgaggtg
     5341 tccgcacccg gtggcggcgt cgccacagcc tcgccgcacg ccacagcggc gagcgcgatc
     5401 acgagactcc cgatgaggtt ccgcactccg ccgatcccgt ttcgcagcat agcccgggcc
     5461 ttcgcgctcc cgccacccgc gctcgcgccc tcagcgagcc gcgagggcgg ctacgagcgc
     5521 gaacgtctcg ccgagctcga tcgacgcccc gaacacgtcg ccgctcacgc cgcccagtcg
     5581 ccggttcgac cacgagccga tgaggatgga cgcggccacg acggcgacga tcatgcccgg
     5641 cgtggcgatg gcggcgctgg ggatggcgat ggcgagggcg acggccgacg cgatggcgac
     5701 cgtgcgggca ccgatcccgg cggtccacgt cccggctccg ccgcccgcgt agggaagccg
     5761 ccacgccatg gcgaccggca ccacgcgcga gagcgtcccc gcggcgacca ccggccacgg
     5821 gaacccggtg accatgagcg cggacagcgt cgaagtcttc agcagcaggt cgagcacgac
     5881 cgcggcgacg ccgaaggtcc ccaaccgcgg gtcgcgcatc gcgcgcgccg gatcgcttcc
     5941 ggccagcgag gccccgatcc cgtccgcgac gtcaccgagc ccgtcgagat ggagcgcggc
     6001 ggtcaccgcg acgccggccg cgatgccgag caccgcggcg acgaacgtcg gcagtacctg
     6061 ggccgaaccc caggcgacga ccgcgacgag cgcgccgacc accccgccga ccaccggcaa
     6121 cagcgccgcg ctgcggcgga ggtcgcgtgg gccaagctcg acggcgcgac cgaccgggac
     6181 cgcgctcagg aacgagagcg ccgcgaggac gggcctgatc cgctcacgca tcgccggcgg
     6241 ccgacctggg ttcgaccgcg ggtacgggtc cgtcgacgcc ggcggcgtcg aacgtggcca
     6301 tgtcggtcag gatcgcgatc gccgagcgca cgagcgggag cgcgagcgcc gcgccggtgc
     6361 cctcgcccag gcgcagcccg aggtcgagca ggggttcgag cccga
//
LOCUS       DATBRK010000418         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_380142, whole genome shotgun sequence.
ACCESSION   DATBRK010000418 DATBRK010000000
VERSION     DATBRK010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380142"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VIH70_12350"
     CDS             <1..349
                     /locus_tag="VIH70_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117200.1"
                     /translation="WTTSNGWQIGQQYFMLKSRPTAEQLAENAKSKPQKAEKKGFMAS
                     MMERAEQQRKLRGDSPPKPGSGKPGNPKPPAPKGGTGGSTGSGPDKPGSNPKQSGSGG
                     NGRGGDPKKRPKR"
     gene            315..983
                     /gene="jag"
                     /locus_tag="VIH70_12355"
     CDS             315..983
                     /gene="jag"
                     /locus_tag="VIH70_12355"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEY8117201.1"
                     /translation="MEEIRRSAPSVEEAVEAALGELGLTEQEADVQVVQEPRSGVFGV
                     GAQEAIVVVRPRAKPEGDLSEDDLDEQAEIATEFLEGMLERMGITATVEPAFEDGTMY
                     VDVIGPEPDDEDMGLLIGRHGQTLEAIQELARVAVGQRTGLPARVVVDIEDYRKRQIE
                     RLSARVREIALRVARTGRPETLEPMNPFLRKMVHTAVAGIDGVGSSSEGEGAERRVVI
                     HRAR"
     gene            1037..1645
                     /locus_tag="VIH70_12360"
     CDS             1037..1645
                     /locus_tag="VIH70_12360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEY8117202.1"
                     /translation="MSDIETLRAQAGSLGVALDRAQASRLVRFEELLLARAIPLGAVS
                     RSDATRIRERHILDSLRAVPVVEGADLAADLGSGAGLPGVVVAIALPRLSMLLVERRP
                     RRAALLELLVEELGVSNTTVFAGPVAEMPGRVGVALARAFAPLDEAWAQARGILGPGG
                     RLVYFAGAESAIPAAPEGSAILDVLRTPVLESSGALVIMTGQ"
     gene            1822..3168
                     /locus_tag="VIH70_12365"
     CDS             1822..3168
                     /locus_tag="VIH70_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8117203.1"
                     /translation="MANQKGGVGKSTTAVSLGASLAELGYRVLVVDLDPQGNASTGLG
                     IRHEEREVTVYDVLADEAPIERAIVPTPVDNLFAIPSTIDLAGAEIELVSQFSRELRL
                     KKAIEPLKRDAYDFVFLDCPPSLGLLTVNALAAAEELIVPIQCEYYALEGLGQLLRNV
                     RLVQQNVNTDLRLTGIVMTMFDPRTKLSEQVVAEVRRYFGDRVYRTIIPRTVRLSEAP
                     GFGQPITVYDPHSKGAESYRDLAQEVASRLPDERPLPNVDDVPSVVIPRPEAQPGRAP
                     RPLVGPEDLPAPSHSEPVAASPGDEDLAAMPDAGGDPDADPTGGASDEPRPGAAEPVA
                     DEPPRADEPPRANESPTSDESPRADEPPRDDVPPRDDVPPRAIDLPEGEDVVHPPVES
                     GPTVDQGSRDPIDEPMDLNGDRTQAEPEDEMQLDPGGSTDPGGGKRRRGLFRRGGT"
     gene            3168..>3406
                     /locus_tag="VIH70_12370"
     CDS             3168..>3406
                     /locus_tag="VIH70_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEY8117204.1"
                     /translation="MKRGGLGRGLSALLPGAVEEGFGLLEIPVEAVAPNPRQPRTTFE
                     DDALEALALSIREVGVLQPIVVRKAGNGFELIAGER"
BASE COUNT          545 a         1198 c         1211 g          452 t
ORIGIN      
        1 ctggacgacc tcgaacgggt ggcagatcgg tcagcagtac ttcatgctca agagccgccc
       61 gacggccgag cagctggccg agaacgccaa gtccaagcct cagaaggccg agaagaaggg
      121 gttcatggcc tcgatgatgg agcgcgccga gcagcagcga aagctccgag gcgactcgcc
      181 cccgaagccg ggcagcggga agcccgggaa ccccaaacca cccgcgccca agggggggac
      241 cggcggctcg accgggtccg gtcccgataa gccggggtcc aaccccaagc aatccgggtc
      301 cggaggcaac ggccgtggag gagatccgaa gaagcgcccc aagcgttgag gaggccgtcg
      361 aggccgccct cggcgagctc ggcctcaccg aacaggaggc cgacgtccag gtcgtccagg
      421 agcctcggtc cggcgtgttc ggggtcggcg cccaggaggc catcgtggtc gtccgtccgc
      481 gcgccaagcc cgaaggcgat ctgagcgagg acgacctcga cgaacaggcc gagatcgcga
      541 ccgagttcct cgagggcatg ctcgaacgga tgggcatcac cgcgaccgtc gaaccggcgt
      601 tcgaggacgg cacgatgtac gtggatgtga tcggccccga acccgacgac gaggacatgg
      661 gcctgttgat cggccggcat ggccagacgc tcgaggcgat ccaagagctc gcccgcgtag
      721 ccgtcggcca gcggaccggg ctcccggctc gggtggtcgt cgacatcgag gactaccgca
      781 agcgccagat cgagcgactc tccgcgcggg tgcgcgagat cgccctcagg gtcgcccgga
      841 ccggtcgccc ggagacgctc gagccgatga accccttcct ccgtaagatg gtccacacgg
      901 cggtggccgg gatcgatggg gtgggcagct ccagcgaggg cgagggtgcg gaacggcgcg
      961 tcgtcatcca tcgagcgagg tagccccggc gcccggacgt tggcgggctc aaggtgtcgt
     1021 ttcacgtgaa acaccgatga gtgacatcga gacgctccga gcacaggccg ggagcctcgg
     1081 cgtcgcgctc gatcgggcgc aggcatcacg cctggttcgg ttcgaggagc tgctcctcgc
     1141 ccgggccatc ccgctcggcg ccgtctctcg ttcggacgcg acccggatca gggagcgcca
     1201 catcctggat agtctgcgag cggtcccggt ggtcgagggc gccgacctgg ccgcggacct
     1261 gggctcgggc gccgggctcc ccggcgtcgt cgtggccatc gcgctccccc ggctttcgat
     1321 gctgctggtc gagcgccgtc cgcggcgagc cgccctcctc gagcttttgg tggaggagtt
     1381 gggcgtgtcg aacacgaccg tcttcgccgg tccggtcgcg gagatgccgg gccgggtggg
     1441 cgtcgccctg gcgcgggcct tcgcgccgct ggacgaggcc tgggcccagg ctcgtgggat
     1501 cttgggaccc ggggggcggc tcgtctactt cgcgggagcc gagagcgcga tcccggcggc
     1561 gcccgagggc tccgcgatcc tggatgtcct gcgcacgccc gtgcttgaaa gctcgggcgc
     1621 cctggttatc atgactggtc agtgacggag cccacttcct ctcgggcagc tcgttcgtcc
     1681 ttcaagccga agaccgaatc cacacgagcg accgaggctt cagggacgca atcgcctgcc
     1741 gcgcaacgcc gtacggaggc cgccggctcg gcggcgtcga acccggacgc ccggcgccca
     1801 ggtcgggcgc ggatcatcgc ggtggccaac cagaagggcg gcgtgggcaa gagcaccacg
     1861 gcggtcagtc tgggcgcgtc cctcgccgag ctcggctaca gggtcctggt cgtcgacctc
     1921 gacccccagg gcaacgcctc gaccgggctg gggatccgtc acgaggagcg tgaggtcacc
     1981 gtctacgacg tgctggcgga cgaggcgccg atcgagaggg cgatcgtccc gacgccggtc
     2041 gacaacctgt tcgcgatccc ctccacgatc gacctcgccg gcgcggagat cgagctcgtg
     2101 agccagttct cacgcgagct gcgcctgaag aaggccatcg agccgctcaa gcgcgacgcc
     2161 tacgacttcg tgttcctgga ctgcccgccc tcgctcggcc tgctgaccgt gaacgcgctc
     2221 gccgcggccg aggagctcat cgtgccgatc cagtgcgagt actacgcgct ggagggactg
     2281 ggccagctcc tgcgcaacgt gcggctggtg cagcagaacg tcaacaccga cctgcggctg
     2341 accgggatcg tgatgacgat gttcgacccc aggaccaagc tctccgaaca ggtcgtcgcc
     2401 gaggtccgtc gctacttcgg ggaccgcgtg taccggacga tcatcccccg gacggtcagg
     2461 ctctccgagg caccgggctt cgggcagccg atcacggtct acgacccgca ttcgaagggc
     2521 gccgagagct accgggacct cgcccaggag gtcgcctccc gcctccccga cgagagaccg
     2581 ctcccgaacg tcgacgacgt ccccagcgtc gtgatccccc ggcccgaggc acagcccggg
     2641 agggccccgc gccccctggt cggccccgag gatctgcccg ccccgagcca ttccgagccg
     2701 gtcgcggcct cccccggcga cgaggacctg gccgcgatgc ccgacgccgg aggcgatccc
     2761 gacgccgacc cgaccggggg cgcctccgac gagcctcggc ccggggccgc cgagcctgtg
     2821 gccgacgagc cacctcgagc cgacgagcca cctcgagcca acgagtcgcc tacatccgac
     2881 gagtcacctc gagccgacga gccgcctcga gacgacgtcc cgcctcgaga cgacgtcccg
     2941 cctcgagcca tcgacctccc cgagggcgag gacgtggtac accctcccgt ggagtcgggt
     3001 ccgaccgtcg accaggggtc cagggatccg atcgatgagc cgatggatct gaacggcgac
     3061 cgaacgcaag ctgaaccgga ggacgagatg cagctcgacc cagggggttc gaccgatccg
     3121 ggcggcggga agcgccgccg cggtctcttc cgcagaggag gaacgtgatg aagcgaggag
     3181 gtctcggccg ggggctgtcg gcgctgcttc cgggcgccgt cgaggaggga ttcggtctgc
     3241 tcgagatccc ggtcgaggcg gtcgccccga acccccggca gcccaggacc acgttcgagg
     3301 acgacgcgct cgaggcgctc gcgctctcga tccgcgaggt cggcgtcctg cagccgatcg
     3361 tcgtgcgcaa ggccgggaac ggcttcgagc tgatcgcggg ggagcg
//
LOCUS       DATBRK010000419         6056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_679812, whole genome shotgun sequence.
ACCESSION   DATBRK010000419 DATBRK010000000
VERSION     DATBRK010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679812"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VIH70_12375"
     CDS             complement(<1..1043)
                     /locus_tag="VIH70_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY8117205.1"
                     /translation="MATIRAKEGGGMFGQRKKRRSAVAARRRLASLFVVAFSLLMIVL
                     VLAPAAGAVTQCTFAGTTATVTIDSGNSAVVGVNTAPTPDEIIFDNDTTLAGALQCGI
                     ANVTNTNLIDVDGSTGSETFVVALSGGAFTPNNDFNINLGTGTDRVTIVGSGGADAIG
                     FASATSITLGGGTNITLASGSVEAFTVNAGLGDDTVSGGSFATAITINGDAGNDILTG
                     GSGVDTINGGAANDTINGGTGTANDALTGGDGTDRLTYAGVTVALTVNLATTTAQNTG
                     GGGTDTISTFENLTGGSSADTLTGSSVANSISGGDGDDTINAGDGNDSLQGDAGDDTL
                     IGGAGDDTINGGDG"
     gene            1506..1634
                     /locus_tag="VIH70_12380"
     CDS             1506..1634
                     /locus_tag="VIH70_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117206.1"
                     /translation="MNTHFPLFRIAFAAAAAVLIFLMVHAALEGISGTANVLAGLQ"
     gene            complement(1723..1799)
                     /locus_tag="VIH70_12385"
     tRNA            complement(1723..1799)
                     /locus_tag="VIH70_12385"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1763..1765),aa:Ala,seq:cgc)
     gene            complement(1840..2385)
                     /locus_tag="VIH70_12390"
     CDS             complement(1840..2385)
                     /locus_tag="VIH70_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117207.1"
                     /translation="MAVRKETIKQQFNEALPAVLEPGERVLGGAYGVSGPNPLWAQGL
                     LGLAGFLIFGMRYYYVALTDRRLIFMNASFWTSRPKGFAWSDPRDSITIGDLQTDNKL
                     WNWGRISSPTKQNLRMNFHAFWRPELKAMGDLLADRIVGPRLAPGAAGDPTPSAGPGA
                     SAPPGPPPPPAAPPPLPPSEG"
     gene            complement(2388..3029)
                     /locus_tag="VIH70_12395"
     CDS             complement(2388..3029)
                     /locus_tag="VIH70_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEY8117208.1"
                     /translation="MYLDGVSRDLRGRFDARVQARERALPASRRAIRSCANAIRAIHR
                     GEFELAHRLMDEARAAIDEGLAAVAEHPDIRFAGYLQDAQKEYTEARLTEVIVDGERE
                     VPTPDALGVEDAPYLNGMAEAIGEARRRVLDLLRAGRVDEGDAVLAAMEDVYGVLVTM
                     DYPDAITGNLRRATDVARSLLEKTRGDLAVARVSQDLHDALDRHAREVLGKGD"
     gene            complement(3040..3615)
                     /locus_tag="VIH70_12400"
     CDS             complement(3040..3615)
                     /locus_tag="VIH70_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117209.1"
                     /translation="MGWLLLAALVVLWAAFLLPSWANPSKTDRSMKDFERNMDMLAET
                     ESPGRWIVTPRKGTTFIGLRARAQARARERRRRVLVVLIESLVLTALIGLVPPLRAMW
                     YATATLLVLLASYVWALLTMKARADPHDPVRSRETAATAPASPRPARQRYAADASNRT
                     ARRSFNGLPAFGDDLANIVVRPAAEVDAARV"
     gene            complement(3772..4227)
                     /gene="moaC"
                     /locus_tag="VIH70_12405"
     CDS             complement(3772..4227)
                     /gene="moaC"
                     /locus_tag="VIH70_12405"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY8117210.1"
                     /translation="MEREAVARIVEVGGKRATDREAVAECVVSMTREACAHLVGGTPK
                     GNPLEAARIAGVMGVKRTPDLLPFCHPVAVTGAEVLVEPDEASGTIRVRTTVRAHDRT
                     GVEMEALTAAAVAALSLYDTAKAFDRAARIDGLRLLEKRGGKSGHYSAE"
     gene            complement(4233..5495)
                     /gene="glp"
                     /locus_tag="VIH70_12410"
     CDS             complement(4233..5495)
                     /gene="glp"
                     /locus_tag="VIH70_12410"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY8117211.1"
                     /translation="MATPGHVHDHDSGEGLVPFDEARERVLSTIKPLQPLALPLTEAY
                     GCVLAESLVAERDIPDFASSAMDGFAVRSSEIASATTTAPVELRIVGRAMIGERPEGT
                     VGGGEAFAIATGAPVPAGADTIAPIETCEVVGGNVRVLAPSIGGKHIRPSGEDVRSGE
                     TLVQAGRRLQAPELGLLATAGFAHPLVHPRPRVVVLSTGDELIPPSRKPDYGQVRDSN
                     AYTLFAALRETGAVPTIAGIVPDDVNAFKEVVFSHLTQADAFISSGGVSVGERDVVKA
                     AFFRRGEVDFYRVAMQPGMPQGFGEIEGKPYFGLPGNPVSVFVSFETFIRPALMKMMG
                     RRQLFRPEVTARLEEDVTGPIGKTQFARVRVRRTKEGWVATPTGGRGSNLIATVAKAN
                     GLAIVPPGTQTASAGSQVKVVLFRSMED"
     gene            complement(5546..>6056)
                     /locus_tag="VIH70_12415"
     CDS             complement(5546..>6056)
                     /locus_tag="VIH70_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8117212.1"
                     /translation="FAVLLGDDLIVEHTRLLQEMLGVHEKYGRSVLGAMEVSRDEISL
                     YGCIEPELVENEHLARVRSIVEKPTPEEAPSNLAAIGRYVLTPEIFECLRRTQPGVGG
                     ELQLTDAINLLAQEQAVYAYVFEGGRFDVGNKLDYLKATIELAITREDIGKDFRDWLS
                     GFVERKKLI"
BASE COUNT          921 a         2148 c         2013 g          974 t
ORIGIN      
        1 ccgtcgccac cgttgatggt gtcatcacca gcaccaccga tcagagtgtc gtcgccggca
       61 tcaccctgca gactgtcgtt cccgtcacca gcgttgatgg tgtcgtcgcc gtcgccaccg
      121 ctgatcgagt tggcgacgct acttccggtc aaggtgtcgg cactcgatcc gccggtgagg
      181 ttctcgaagg tcgagatcgt gtcggttccg cccccgccgg tgttctgcgc ggtcgtggtg
      241 gcgaggttga cggtcagcgc gaccgtgaca cccgcatagg tcagcctgtc cgtgccgtcg
      301 ccaccggtca gtgcgtcgtt cgccgtgccc gtaccgccgt tgatcgtgtc gttcgccgcg
      361 cctccgttga tggtgtccac gccactcccg ccggtgagga tgtcgtttcc cgcatcgccg
      421 ttgatggtga tggccgtcgc gaaggaccca ccgctcacgg tgtcgtcacc tagcccggcg
      481 ttcacggtga atgcctcgac cgatcccgaa gcgagcgtga tgtttgtgcc cccgccgagc
      541 gtgatgctcg tcgcggatgc gaagccaatc gcgtcggcgc cgccggaccc gacgatcgtg
      601 accctgtcgg tgcccgtccc caggttgatg ttgaagtcgt tgttcggtgt gaaggcaccg
      661 ccagacaagg caaccacgaa ggtttcgctg ccggtactcc catccacgtc gatcagattc
      721 gtgttcgtca cgttcgcaat gccgcattgg agtgcgcccg caagcgtcgt gtcattgtcg
      781 aagatgatct cgtcgggtgt gggcgcggtg ttcacgccga cgaccgcgga gttccccgaa
      841 tcgatcgtca ccgtcgcagt ggtacccgcg aacgtgcatt gcgttacggc gccggccgct
      901 ggtgccaaga ccagcacgat catcagtagc gagaaggcga cgacgaagag cgaagccagc
      961 cgccgcctcg ctgcgaccgc agagcgcctc ttcttccgtt gaccgaacat ccctccccct
     1021 tccttcgcgc ggatcgtggc cacgaggagc ctccctcgag gccagaacgg cgaccacagg
     1081 tcgacaacct cgaatgtccc gcaacagaag agaacgggag tcaggcccga aggttgccct
     1141 ctggtccgtt cactgccgcc ctctgcgcgg cctcggtcgc gaggacctcg tgttcacctc
     1201 gctcgcggcg atcggggcgc tgccgtggac gccggtgagg acgccatcgc gacaagctcc
     1261 gggccgtacc cgttacctcc cgttgggcca aggatcacgg atgcggcgga gcatcttcgg
     1321 gtcgccgcct gttgcatcca caaggacccg ggacggcctc gtcgaaccct acgggcgacg
     1381 actattggcc tttcggcgaa cgcgtttggc ccaacggctt atcgcctgca acgccgccga
     1441 tccgtaccct ttgggggacg aaccgtatcc cccaaaaggg gccccttacc gaggaggtag
     1501 cccacgtgaa cactcacttc cctctcttcc ggatcgcgtt cgccgccgcc gcggccgtct
     1561 tgatcttcct catggtccac gcggcgctgg aaggaatctc cggcacggcc aacgtgctgg
     1621 ccgggttgca gtagcgctgc cggattggat cgggtttttt gtcaggccac cgagcgctca
     1681 gagacaccag ggcgataacc atgattgacc tgggagttct cttggtggag gtgaggggat
     1741 ttgaacccct gacccctgcc gtgcgaaggc agtgctctac cggactgagc tacacccccg
     1801 gaaccgcgcc agggtaacag aggttcccgc gcgggccgct caaccctccg acggcgggag
     1861 aggcggcggc gcggccggtg gcgggggcgg tcccggcgga gcggacgccc ccggaccggc
     1921 ggacggggtc ggatcgccgg cggctcccgg ggcgaggcgc ggcccgacga tccggtcggc
     1981 cagcaggtcg cccatcgcct tgagctccgg acgccagaac gcgtggaagt tcatccgcag
     2041 gttctgcttg gtcgggctcg agatcctgcc ccagttccaa agcttgttgt ccgtctgcag
     2101 gtcgccgatc gtgatcgagt cccgcgggtc ggaccaggcg aaccccttcg ggcgcgaggt
     2161 ccagaaggaa gcgttcatga agatcaggcg ccgatcggtg agcgccacgt agtagtagcg
     2221 catgccgaag atcaggaacc cggcgaggcc gagcaagccc tgggcccaca gcggattcgg
     2281 tcccgagacg ccgtaggcgc cgccgaggac ccgctcgccc ggctcgagca ccgccgggag
     2341 cgcctcgttg aactgctgct tgatcgtctc cttgcgaacg gccacgatca gtctcctttc
     2401 cccagcacct cgcgcgcgtg ccggtcgagc gcgtcgtgga ggtcctgcga gacgcgcgca
     2461 accgcgaggt cgccccgcgt cttctcgagc aggctgcgcg cgacgtcggt cgcgcgacgc
     2521 aggttcccgg tgatcgcatc ggggtagtcc atcgtcacga ggaccccgta cacgtcctcc
     2581 atcgccgcga ggaccgcgtc gccctcgtcg acgcgcccgg cgcgcaacag gtcgaggact
     2641 cgccggcgcg cctcgccgat cgcctcggcc atcccgttca ggtacggcgc gtcctcgaca
     2701 ccgagggcgt cgggcgtcgg gacctccctc tcaccgtcca cgatcacctc ggtgagccgg
     2761 gcttccgtgt actccttctg cgcgtcctgc aggtagccgg cgaaccggat gtccggatgc
     2821 tcggcgaccg cggcgagtcc ctcgtcgatc gccgccctcg cctcatccat gagtcggtgc
     2881 gcgagctcga actcgccgcg gtggatcgct cggatcgcgt tcgcgcagga gcggatcgct
     2941 cgacgagacg ccgggagcgc gcgctcgcga gcctgcacgc gcgcgtcgaa gcgccctcga
     3001 agatcccggg agacgccgtc cagatacacg ggacggcgtt cagacgcgag ccgcgtcgac
     3061 ctcggccgcg ggcctgacca cgatgttcgc gaggtcgtcg ccgaacgcgg ggaggccgtt
     3121 gaacgaccgg cgtgccgtgc ggttggaggc gtcggccgcg taccgctgcc gagccgggcg
     3181 cggactcgcg ggtgcggtcg ccgcggtctc gcgcgaacga accgggtcgt gcgggtcggc
     3241 ccgcgccttc atcgtcagca gcgcccacac gtagctcgcg agcagcacga gcaaggtcgc
     3301 ggtcgcgtac cacatggcgc gcagcggcgg gacgagaccg atcagcgcgg tgagcaccag
     3361 gctctcgatc agcaccacga gcacgcggcg tcggcgttcg cgcgccctcg cctgcgcacg
     3421 cgcacgcaag ccgatgaacg tcgtcccctt gcggggcgtg acgatccacc ggcccggcga
     3481 ctcggtctcc gccagcatgt ccatgttccg ctcgaagtcc ttcatgctcc tgtccgtctt
     3541 gcttgggttc gcccacgatg ggaggaggaa cgcggcccac aggacgacca gcgccgcgag
     3601 cagcagccaa cccacctacc taacccccgt gtgttcggat ccccggtccg gcacaagatg
     3661 ttgggctggg tcagcaaggt agcatcgagg ctaagcaggt acaaacacct cgcgcaagtt
     3721 gctccgaagg tcatgcatcg acacatctcg cgcgatcgtc tcgcgagcga ctcactccgc
     3781 cgagtagtga ccgctcttgc cgccgcgctt ctcgagcaga cgcaggccgt cgatgcgcgc
     3841 cgcgcgatcg aacgccttcg ccgtgtcgta gaggctgagc gcggccaccg cggccgcggt
     3901 gagcgcctcc atctcgacgc cggtgcgatc gtgcgcgcgt acggtcgtgc gaacgcggat
     3961 cgtcccactc gcctcgtcgg gctcgacgag cacctccgcg ccggtcaccg cgaccggatg
     4021 gcagaacggc aacaggtcgg gggtccgctt gacgcccatc acaccggcga tccgagcggc
     4081 ctcgagcggg ttgcccttcg gcgtaccccc gaccaggtgc gcgcaggctt cgcgcgtcat
     4141 cgagacgacg cattcggcga cggcctcgcg gtcggtggct cgcttgcctc cgacctcgac
     4201 gatgcgcgcc acggcctcac gctccatcga catcagtcct ccatcgaacg gaacaggacg
     4261 accttcacct ggcttccggc gctcgcggtc tgggtcccgg gcgggacgat cgcgaggccg
     4321 ttcgccttcg cgacggttgc gatcaggttc gagccgcggc caccggtcgg cgtggccacc
     4381 cagccctcct tggtccggcg aacgcgcacg cgcgcgaact gcgtcttgcc gatcggcccg
     4441 gtgacgtcct cctcgagcct ggcggtcacc tcgggccgga agagctgccg gcgtcccatc
     4501 atcttcatga gcgccggccg gatgaaggtc tcgaagctga cgaagacgct caccgggttg
     4561 cccggcaggc cgaagtacgg cttgccctcg atctcgccga acccctgggg catccccggc
     4621 tgcatcgcga cccggtagaa gtcgacctcc ccccggcgga agaacgcggc cttcacgacg
     4681 tcccgctccc cgaccgacac cccgcccgag gagatgaacg catcggcctg cgtgaggtgc
     4741 gagaacacga cctccttgaa cgcgttgacg tcgtccggaa cgatgccggc gatcgtcggc
     4801 accgcgccgg tctcgcgcag cgccgcgaac agcgtgtacg cgttcgagtc acggacctga
     4861 ccgtagtcgg gcttccgcga cggagggatc agctcgtccc cggtcgagag cacgacgaca
     4921 cgcgggcgtg gatgcacgag cgggtgcgcg aagccggcgg tcgcgagcag cccaagctcc
     4981 ggcgcttgga ggcggcgccc cgcctgcacg agcgtctcgc cggatcgaac gtcctcgccg
     5041 ctcggacgga tgtgcttgcc cccgatcgac ggggcgagga cgcgaacgtt gccgcccacg
     5101 acctcgcagg tctcgatcgg cgcgatcgtg tcggcgcccg cggggacggg cgcgccggtc
     5161 gcgatcgcga acgcctcgcc gccgcccacc gtgccctcgg gtcgttcccc gatcatggcg
     5221 cgaccgacga tgcgcagctc gacgggcgcg gtcgtcgtcg ccgacgcgat ctccgaagag
     5281 cgcaccgcga acccgtccat cgccgaggac gcgaagtcgg ggatgtcgcg ctcggcgacg
     5341 aggctctcgg ccagcacaca gccgtaggcc tcggtcagcg gcagcgcgag cggttgaaga
     5401 ggcttgatcg tcgacaggac gcgttcgcga gcctcgtcga acgggacgag cccctcgccg
     5461 ctgtcgtgat cgtggacgtg accgggcgtc gccatccggg ccagtctatc ggcgggcccc
     5521 agggagccag atcaggacgc cggattcaga tcagcttctt gcgctcgacg aagccggaga
     5581 gccagtcccg aaagtccttg ccgatgtcct cccgggtgat cgcgagctcg atggtggcct
     5641 tcaggtagtc gagcttgttg ccgacgtcga aacgcccgcc ctcgaacacg taggcgtaga
     5701 cggcctgctc ctgggctagc aggttgatcg cgtccgtgag ctgcagctcg ccgccgacgc
     5761 ccggctgcgt ccgccgcagg cactcgaaga tctcgggcgt caggacgtac cggccgatgg
     5821 cggccagatt cgatggcgcc tcctcgggcg tgggcttctc gacgatcgag cgcacgcgtg
     5881 caaggtgctc gttctccacg agctcgggct cgatgcagcc gtacagcgag atctcgtccc
     5941 ggctcacctc catcgcgccg agcacgctcc gcccgtactt ctcgtgcacg ccgagcatct
     6001 cctgcagcag gcgcgtgtgc tccacgatca ggtcgtcgcc gagcagcacc gcgaac
//
LOCUS       DATBRK010000420         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_241981, whole genome shotgun sequence.
ACCESSION   DATBRK010000420 DATBRK010000000
VERSION     DATBRK010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241981"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VIH70_12420"
     CDS             complement(<1..146)
                     /locus_tag="VIH70_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8117213.1"
                     /translation="MRDVTTRNYQSIGEVLVSVKTEFPDITISKIRFLESEGLIEPER
                     TPSG"
     gene            complement(143..598)
                     /locus_tag="VIH70_12425"
     CDS             complement(143..598)
                     /locus_tag="VIH70_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8117214.1"
                     /translation="MYCTRCGHPNRDEARYCANCGAVLQEETTIGLNPVEIETEGPED
                     FPFPEDELKAGQALLLVKHGPNAGSTFMIEGDVTSTGRNPESDVFLDDISVSRKHAEI
                     RRHPDGFYIHDLGSMNGTYVNRERVEVTKLAGHDEVQIGRFRLVFFVGE"
     gene            complement(1099..1491)
                     /gene="gcvH"
                     /locus_tag="VIH70_12430"
     CDS             complement(1099..1491)
                     /gene="gcvH"
                     /locus_tag="VIH70_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY8117215.1"
                     /translation="MEFPEDLRYTTEHEWAREEGSNRVRVGITDFAQDALGDVVYVDV
                     PQTGATVSANQPFTEVESTKSVSDVYAPVSGTVVERNPLLDERPELINEQPYGDGWLV
                     VLEVADAAPLSELMDAAAYRAFVEQQAD"
     gene            complement(1597..2199)
                     /locus_tag="VIH70_12435"
     CDS             complement(1597..2199)
                     /locus_tag="VIH70_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY8117216.1"
                     /translation="MSEPAGSDRVLTIPNLISSLRIALIPAFVVLIVDPDTTRVGLVL
                     FVVVVATDWIDGALARATGQVSDLGKVLDPVADRLAIAAGLIALVVVDAFPLWAALLI
                     LVRDVAVLIAGLVLLAKRRARIEVRYLGKVATFALMTAIACIAWGSLGYPLAPAALAC
                     GWAIYAAGILEYYAATLLYVGDLRRAWRAPLLERSADHAT"
     gene            complement(2223..2297)
                     /locus_tag="VIH70_12440"
     tRNA            complement(2223..2297)
                     /locus_tag="VIH70_12440"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2260..2262),aa:Pro,seq:ggg)
     gene            complement(2351..>2872)
                     /locus_tag="VIH70_12445"
     CDS             complement(2351..>2872)
                     /locus_tag="VIH70_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8117217.1"
                     /translation="ADVAVLVLDASQGFTGEDKRIAARVMEVGRGLVVVANKWDLIEE
                     RDRTFKELTELLSPYARAPVLRTSAIGGTGVHRLPAALSQVHDRWNHRAPTARVNEVV
                     AAAQAEQQAPGGIRYRYATQVAAGPPRFVVFGAGPPAASYQRFLEGRLRRALHLEGVP
                     IGLRFRTGRRRAR"
BASE COUNT          445 a          995 c          999 g          433 t
ORIGIN      
        1 tagcccgacg gcgtccgctc gggttcgatg aggccctcgg actcgaggaa ccggatcttg
       61 ctgatcgtga tgtcggggaa ctccgtcttg accgacacga gcacctcgcc gatcgactgg
      121 tagttgcggg tcgtcacgtc cctcactccc ccacgaagaa cacgagccgg aaccggccga
      181 tctggacctc gtcgtgaccg gcgagcttgg tgacctccac gcgctcgcgg ttcacgtacg
      241 tgccgttcat gctcccgaga tcgtggatgt agaacccgtc gggatggcga cggatctcgg
      301 cgtgcttgcg actcacgctg atgtcgtcga ggaagacgtc gctctcgggg ttgcgccccg
      361 tgctcgttac gtccccctcg atcatgaagg tcgatcccgc gttgggcccg tgcttcacga
      421 gcaggagagc ctgcccggcc ttgagctcgt cctccgggaa ggggaagtcc tccggacctt
      481 ccgtttcgat ctcgaccggg ttgaggccga tggtggtctc ctcctgcagg acagcaccac
      541 agttcgcgca gtagcgcgcc tcgtccctgt tcggatgtcc acaccgagtg cagtacacgc
      601 tcgacccccc gttgccctcg tctcacctcg acggtggggt cccgaggctc aacctcgacc
      661 tcgagactcg ctcgaaacta ccgtcccgcc cacgaagggt caaccgcacc ccgcgcgggc
      721 tcgaaggcag ctcgacggtt cgggactcga tcgagtgcaa cggtcgacgg gagcaagacc
      781 caagcgtcga gggtcgaccg gggacagggt ccacggtggc ccgggggcaa cctgtagtgg
      841 tcgcacgctg cacctcgagg acggctcggt gcgaccggga tcggtggtcg aggttcgggt
      901 gtcctgggag gtctcggcct cgaccggcgt ccgaggtcga cctccagcca cgaggggttc
      961 ccgttcaggc tgggatcgaa ggtgcggaca cgacacgcga tccgaggcgg atctcgagga
     1021 ggctggtcgg gcctcgaccg tcggtgacag gtggacgtcg ggggcggctc gacgccgacg
     1081 tcgggcacgc ctgcgggctc agtcggcctg ttgctcgacg aacgctcgat aggcggcggc
     1141 gtccatgagc tcactcaggg gggccgcgtc ggcgacctcg aggacgacga gccagccgtc
     1201 gccgtagggc tgttcgttga tgagctcggg acgctcatcc aggagcgggt tccgctcgac
     1261 gaccgtcccg gagacgggcg cgtacacgtc cgagaccgac ttggtcgact cgacctcggt
     1321 gaacggctgg ttcgcgctca cggtcgctcc cgtttgcgga acgtcgacgt agacgacgtc
     1381 gccgagcgcg tcctgcgcga agtcggtgat gccgacgcgg acccgattcg agccctcctc
     1441 gcgcgcccac tcgtgctccg tcgtgtaccg caggtcctcg gggaactcca tgcgtctctc
     1501 cttgcggtgt ccctctgccg aaagtccttc gctcgtcaca tcaccaccgc gtggggctgg
     1561 aaggtagcac gtcggacggg atggaccggc accagctcat gtcgcgtggt cggccgagcg
     1621 ttcgaggaga ggcgcccgcc aggcacgtcg gaggtcgccc acgtacagga gggttgccgc
     1681 gtagtactcg aggatccccg cggcgtagat cgcccacccg cacgcgagcg ccgcgggcgc
     1741 gagtgggtag ccgaggctcc cccacgcgat gcaggcgatc gccgtcatca acgcgaacgt
     1801 cgcgaccttg ccgagatagc gcacctcgat ccgcgctcgg cgcttcgcga gcaacacgag
     1861 cccggcgatc agcaccgcca cgtcgcggac caggatcagg agcgccgccc acaggggaaa
     1921 ggcgtcgacc accacgaggg cgatcaggcc ggcggcgatc gccagccggt cggcgaccgg
     1981 gtccaggacc ttgccgaggt cggagacctg ccccgtggca cgggcgagcg ccccatcgat
     2041 ccagtcggtg gcgaccacga ccacgaacag cacgagcccc acgcgcgtgg tgtcggggtc
     2101 cacgatcagg acgacgaagg cggggatcag agcgatgcgg agcgaggaga tcaggttcgg
     2161 gatcgtcagg acgcggtcgc tcccggccgg ctcgctcatg gcgcggcgaa cctaccactg
     2221 cgtcgggccg gcgggattcg aacccgcgac ctcctgaccc ccagtcagac gcgctaacca
     2281 agctgcgcca cggcccgtgc ccggcgagtc taccggtagg cgagccgagc cggtgcgggc
     2341 ccagggagcg ttaacgtgcg cggcggcggc ccgtgcggaa tcgaagtccg atcggtacgc
     2401 cctcgaggtg gagcgcccgg cgcagtcgcc cctccaggaa tcgctggtag ctcgccgccg
     2461 gggggccggc gccgaacacg acgaaccggg gcggcccggc ggccacctgc gtggcgtacc
     2521 ggtaacggat cccgcccggg gcctgctgtt cggcctgcgc ggcggcaacc acctcgttca
     2581 cccttgccgt gggcgcccgg tggttccagc gatcgtggac ctgcgacagc gcggccggca
     2641 gccggtgaac gccggtgccc ccgatcgcgg aggtgcgaag caccggcgct cgcgcgtacg
     2701 gggagagcag ctcggtcagt tccttgaagg tccggtcgcg ctcctcgatc aggtcccact
     2761 tgttcgcgac cacgaccagg ccgcgaccga cctccatcac gcgggcggcg atccgcttgt
     2821 cctcgccggt gaacccctgc gaggcgtcga gcaccagcac agccacgtcc gc
//
LOCUS       DATBRK010000421         5530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2405580, whole genome shotgun sequence.
ACCESSION   DATBRK010000421 DATBRK010000000
VERSION     DATBRK010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405580"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..624
                     /locus_tag="VIH70_12450"
     CDS             256..624
                     /locus_tag="VIH70_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY8117218.1"
                     /translation="MEIRLRYFEGCPHWKIAQDRLREALLAEDMADVELILERLETPE
                     DAERLRFIGSPTILLDGRDPFAGATLAIGLTCRVYQTPEGLAGSPTPEQLRAALRGGL
                     IQSRNERTPLQSKTDGGDYP"
     gene            624..863
                     /locus_tag="VIH70_12455"
     CDS             624..863
                     /locus_tag="VIH70_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY8117219.1"
                     /translation="MYGPWDGGWGFGMMFMSLLFLALIVVGVVFVVRSFSHGGQAPPR
                     SDGSRALDILDERFARGEIDQQEYEERRRALTGGR"
     gene            868..1443
                     /locus_tag="VIH70_12460"
     CDS             868..1443
                     /locus_tag="VIH70_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117220.1"
                     /translation="MRQRTLGVVLIALGLVGMLGIGWAFASGSDGYLAAPGWVPFGGI
                     RGGPTCTPPTLPGQAVDVVLSDMGGMMSGGTMSGARMMNVAASRSSIPAGDASFRVWN
                     AGMMVHELVVLPLPPDGVVGTRTVGSDGRVSEEGSLGEASRTCGEGAGEGIAPGVAGW
                     VILHLDPGRYELICNLPGHYAAGMFTELDVR"
     gene            complement(1484..2008)
                     /locus_tag="VIH70_12465"
     CDS             complement(1484..2008)
                     /locus_tag="VIH70_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8117221.1"
                     /translation="MGTGTILVVDDERKIRDLVRSSLELEGDSVLLADTGERALESAS
                     RSDPYLVVLDLGLSDLAGEEVARTLRTGSNVPIIVLTAKAAEDDRVTGLRLGADDYLA
                     KPFSLRELVACGFRGRSRAGRIAQRPRAPQVDRPRALTCSHLMAGRHAVPPQSVVGWP
                     HGMLIAIGFFVGAC"
     gene            complement(2010..2273)
                     /locus_tag="VIH70_12470"
     CDS             complement(2010..2273)
                     /locus_tag="VIH70_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117222.1"
                     /translation="MGRRAIVLFWGAVAAAVFFLGSAWATAGRLGPGPHALANDVVMA
                     VSVIGLGATTLVAGRIVFVVGRAQRQARARPSDHPVGPSGEEA"
     gene            complement(2273..3358)
                     /locus_tag="VIH70_12475"
     CDS             complement(2273..3358)
                     /locus_tag="VIH70_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY8117223.1"
                     /translation="MSERGASVATTLNEESAPSRWRRAWDALDERLGLKGLQYPIPEH
                     ANKLAYSLGGLTLITFVLMVATGIYLTQFYNPDPALVHESVRRIITSVSFGKFIRGFH
                     YWGAMAMIVLIGLHLLRVFVSGSFKRPREANWLFGVVLAGITATLFFTGTVLKWDQES
                     LEALEHNVEIGKLLGRFGFWFSPTFGGVPFLTRLYVVHIAVLPFLFTIVVAVHLMLVK
                     RLKMAPSPFRKGPEPEPTRPFTKHLAELGALALVLIGILTILAVLAPPPHGPAPIEGI
                     EITKPPWPMLWVYPIEDWVGVVGILWATLAIFLALFLVPFVDRTPERSPRKRLPMMIA
                     AGVVVVTIIGLIIYAAVQPVASHIGMS"
     gene            complement(3355..3840)
                     /locus_tag="VIH70_12480"
     CDS             complement(3355..3840)
                     /locus_tag="VIH70_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117224.1"
                     /translation="MAVGSFAILAPPALAHEGEEAVPAITDVQEAIAILAEHPGVFPT
                     AEVAEHAMDKVGDALESNDSRGVLLDLVTEAKDALDAGRADDALTLLERSIGACPGAP
                     VIEPQDAPRTPPPLVSPCPSVAHIQALDRTPVRGTQEPVLIAVGAVLILAGLGLARRI
                     R"
     gene            complement(3886..4155)
                     /locus_tag="VIH70_12485"
     CDS             complement(3886..4155)
                     /locus_tag="VIH70_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY8117225.1"
                     /translation="MGDNHDGILKRLRRIEGQVSGIRKMQEDGRYCIDVLDQISAARA
                     GLEAVALLILEDHVNGCVREAIEEGKGEEKAVELLEAVGRFARSV"
     gene            complement(4404..>5530)
                     /locus_tag="VIH70_12490"
     CDS             complement(4404..>5530)
                     /locus_tag="VIH70_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY8117226.1"
                     /translation="FSTAILYAITVVVITCPDALGLATPTAIMVGTGLGAKRGILFKN
                     AVALETSARIQVVVMDKTGTLTKGEPEVTDVVVEGMPESEVLRLVAAVERESEHPLAE
                     AVVRYADDRAVTRVQAESFDNVPGHGALAKVGGHLVAVGNPRLMEREGVDMGPLESKR
                     QDLASGGRTAVIVAVDGQAAAVIGIADAPRPTSAEAISDLRDADVEVVMLTGDNEATA
                     RRIGDQLGVQTVIAEVLPGDKASTIARLQEDGRKVAMVGDGVNDAPALAQADLGIAIG
                     AGTDVAIETADVVLMRSDPLDVPTALAIGRGTLRKMRQNLGWAIGYNSVAIPIAAGVF
                     EPAFGLVLRPEIAALSMSGSSLLVAVNALLLKRLRLPETE"
BASE COUNT          906 a         1906 c         1847 g          871 t
ORIGIN      
        1 cctcgcgacg gtccgctcgg tgatccccgc ttgagccaac gttgcggctc cgccgcgttg
       61 cggagatcct gcttctgatg agcgtcaagc ccgctggtta ggagatcgtg tgaggcgcgg
      121 gactgcgtgc ggcgaccttc cgaccgatcc tgctcacgac cctccaccag actttggtgc
      181 ccgagagcct cggacacctg atccgttctc gggtgcgggc gccgctgccc gccccgagcc
      241 gaacggcttc gcgtgatgga gatccgattg cggtacttcg aggggtgccc gcactggaag
      301 atcgcgcagg atcggctccg cgaggcgctg ctcgcggagg acatggcgga cgtcgagctc
      361 atcctcgagc ggctcgagac tcccgaggac gccgaacggc tccggttcat cggctcaccg
      421 accatcctct tggatggtcg agacccgttc gctggagcga ctctggccat cggtctgacg
      481 tgccgcgtct accagacacc ggagggtctc gcggggtcac ccacacccga gcagcttcga
      541 gcggccctgc ggggcggcct gatccagtca cgcaacgagc ggaccccgct acagtcgaag
      601 acagacggag gtgattaccc gtgatgtacg gaccgtggga cggcggttgg ggcttcggca
      661 tgatgttcat gtcgctcctg ttcctcgcac tcatcgtggt cggcgtcgtg ttcgtcgtcc
      721 ggtccttctc gcacggcgga caggcaccgc cccggtcgga cggcagccga gcgctcgaca
      781 tcttggacga gcgattcgct cgcggtgaga tcgaccagca ggagtacgag gagcggcgac
      841 gagccctgac cggcgggaga tgatcccgtg aggcagcgga cgctcggcgt ggttctcatc
      901 gccctcggtc tggttggcat gctcggaatc ggctgggcgt ttgcctcggg gtccgatggt
      961 tatctcgccg cgcccggctg ggtgcccttc ggcggcattc gcggaggccc gacctgcact
     1021 cctccgaccc tcccgggaca ggccgtcgac gtcgtgcttt ccgacatggg cggcatgatg
     1081 agcggcggca cgatgagcgg cgcgcggatg atgaacgtcg ccgccagccg ctcgtcgatc
     1141 cccgcgggag acgcgtcgtt ccgcgtctgg aacgcgggga tgatggtcca tgagctcgtg
     1201 gtcctacccc tcccgccgga cggggtggtg ggcacccgaa ccgtcggctc cgacggtcgg
     1261 gtgagcgagg agggaagcct cggtgaggcg tcgaggacct gcggcgaagg agccggcgag
     1321 gggatcgccc ctggcgtcgc aggctgggtg atcctgcatc tcgatccggg acgctacgag
     1381 ctgatctgca acctgcccgg gcactacgcc gcgggcatgt tcaccgagct cgatgtccgg
     1441 tagcggcatc gacgggtctc cagccgctcg ggtcgagcgg gcatcagcac gcgccaacga
     1501 agaacccgat cgcgatcagc atgccgtgcg gccagcccac cacggactga ggtggaaccg
     1561 cgtgacgacc ggccatgaga tgtgaacaag tcagggcacg tggccgatcg acttgtggag
     1621 ctcgtggtcg ctgtgcgata cggccggctc gcgatcgacc gcgaaaccca caggcgacga
     1681 gctcccgcag actaaagggc ttcgcgaggt agtcgtcggc gccgaggcga agaccggtca
     1741 cgcggtcgtc ctcggcggcc ttagcggtga gcacgatgat cggcacgttc gagcccgtgc
     1801 gaagcgtccg cgcgacctcc tcacccgcca ggtccgaaag cccaaggtcg agcacgacga
     1861 ggtacggatc tgagcgggag gcggattcga gcgctcgctc gccggtatcc gccaggagca
     1921 ccgagtcgcc ctccagctcg agggaggagc ggaccaggtc gcggatcttc cgctcgtcgt
     1981 cgaccaccag gatggttccg gtacccacgt caggcctcct cgccgctcgg ccccaccgga
     2041 tgatcggagg gcctcgccct ggcctgccgt tgggcccggc cgacgacgaa gacgatccgg
     2101 ccagcgacca acgtggtcgc ccccagccct atcaccgaga cggccatgac cacgtcgttc
     2161 gcgagcgcgt gggggcctgg tccgagtcgc cccgccgtgg cccacgccga gccgaggaag
     2221 aacacggccg ccgccacggc cccccagaac agcacgatcg cccggcgtcc catcagctca
     2281 tcccgatgtg cgaggcgacc ggttgcacgg ccgcgtagat gatgaggccg atgatggtga
     2341 ccacgaccac gccggcagcg atcatcatcg ggagacgctt gcgaggactc cgctccggtg
     2401 tgcgatcgac gaacggcacg aggaacaggg cgaggaagat cgcgagcgtg gcccacagga
     2461 tccccacgac accgacccag tcctcgatcg ggtagaccca cagcatcggc cacggcggtt
     2521 tggtgatctc gatcccctcg atcggcgccg ggccgtgcgg cggcggcgca agcacggcga
     2581 ggatcgtgag gatgccgatc agcaccaagg ccaacgctcc gagctccgca aggtgcttgg
     2641 tgaacggccg agtgggctca ggctcggggc ccttccggaa cggggagggc gccatcttca
     2701 ggcgcttgac cagcatcagg tggaccgcca ccacgatggt gaacaggaac ggcagcaccg
     2761 cgatgtgcac cacgtacagg cgggtgagga acgggactcc cccgaacgtt ggggagaacc
     2821 agaacccgaa ccgaccgagg agcttgccga tctcgacgtt gtgttcgagc gcctccagcg
     2881 actcctggtc ccacttcagc accgtccccg tgaagaacaa ggtggcggtg atgccagcga
     2941 ggacgacacc gaagagccag ttggcctcgc gtggacgctt gaacgatccg gacacgaaca
     3001 cccggagcag gtgcaggccg atcaggacga tcatcgccat cgcgccccag tagtggaatc
     3061 cacggatgaa cttcccgaag gagaccgagg tgatgatccg ccggacgctc tcatgcacca
     3121 gagccggatc cgggttgtag aactgggtca ggtagatccc cgtcgccacc atcaagacga
     3181 aggtgatcag cgtgaggccg ccaagggagt acgccagctt gttggcgtgc tctgggatcg
     3241 ggtattggag acccttcaga ccgagccgct cgtccaaggc gtcccaggcg cgccgccacc
     3301 tgctcggagc tgactcttcg ttcagcgtcg ttgccaccga tgccccccgt tcgctcatcg
     3361 gattctcctc gcgaggccga gcccggccag gatcaggaca gctccgacgg cgatcagcac
     3421 cggctcctgt gtgcccctga cgggggtccg atccagggct tggatgtggg cgaccgacgg
     3481 acagggggaa acaagcggtg gcggcgtgcg cggtgcgtcc tgcggctcga tcactggggc
     3541 cccagggcag gcaccgatcg atcgctcgag cagcgtcagg gcatcatcgg ccctgccggc
     3601 atccagcgcg tccttggcct cggtcaccag gtccagcagc acaccgcggc tgtcgttcga
     3661 ctcgagggcg tctccgacct tatccatggc gtgctccgcg acctccgcag tgggaaagac
     3721 ccccggatgt tcggcgagga tggcgatcgc ctcctggacg tcggtgatgg ccggaacggc
     3781 ctcctccccc tcgtgggcca atgccggggg cgcaaggatc gcaaacgatc cgaccgccac
     3841 gatgacgcca aagggaccgt cgccatgacg gtgtgccgac tcatctcaca cgctccttgc
     3901 gaacctgccg acggcttcca ggagctcgac ggccttctct tcgcccttgc cttcctcgat
     3961 cgcctcgcgg acgcacccgt tcacgtggtc ctccagaatc agcagagcga ccgcctcgag
     4021 cccggctcgt gcggccgaga tctggtcgag cacgtcgatg cagtagcggc cgtcctcctg
     4081 catcttgcgg atgccggaga cctgcccctc gatgcggcgg agccgcttca ggatgccgtc
     4141 gtggttgtct cccatggctg ccgccttcga tcgatcctcg ccggctctcg gatttgccgt
     4201 accccggttg ggcatagcct cgtcgccgaa cgcgcgcctg ccatgggccc aaggtcccgt
     4261 tccaggcggg acgaaaggaa caccccgcct cggggagata gttcgccgcg ccagcagccg
     4321 tacaccagct gacgctcgtc gtccgaaagg accgggctcg ctcggagcgg gcgagatccg
     4381 ttcgacgctc atcctgaagc ctcctactcc gtctcgggca gccggagccg cttcagcagc
     4441 agcgcattca ccgcgaccag gagactggat cccgacatcg acaacgcggc gatctccggc
     4501 cggaggacca acccgaaggc gggctcgaag acgcccgcgg cgatcgggat cgccaccgag
     4561 ttgtagccga tcgcccatcc gaggttctga cgcatcttgc ggagggtccc ccgcccgatc
     4621 gcgagagcgg tggggacgtc cagcggatcg gagcgcatga gcaccacgtc ggcggtctcg
     4681 atggcgacgt cggtgccggc gccgatcgcg atgccgagat cggcctgggc gagcgccggc
     4741 gcgtcgttca cgccgtcgcc caccatcgcg acctttctgc cgtcctcctg tagtcgagcg
     4801 atcgtgctcg ccttgtcgcc gggcagcacc tccgcgatta cggtctgcac gccgagctgg
     4861 tccccgatcc gccgcgcggt ggcttcgttg tcgccggtga gcatgaccac ttcgacatca
     4921 gcatcccgga ggtcggagat agcctccgca gacgtcggac gtggcgcgtc ggcgatcccg
     4981 atcacggcgg cagcctggcc gtccacggcc acgatcacgg ccgtgcgacc tcccgacgcc
     5041 aagtcttggc gtttgctctc cagcggtccc atgtcgacgc cctctcgctc catcaggcga
     5101 gggttgccga cggccaccag gtgacctccg accttcgcga gggcaccgtg gccggggacg
     5161 ttgtcgaagc tctccgcctg cacacgagtc accgcgcgat cgtccgcgta ccggaccacg
     5221 gcctctgcga gcgggtgttc ggactcgcgc tcgaccgcgg ccacgagtcg gaggacctcg
     5281 ctctcgggca tcccctcgac caccacgtcg gtcacctcgg gctcaccctt cgtgagcgtt
     5341 cccgtcttgt ccatcacgac gacctggatc cgggcggacg tctccaacgc gaccgcgttc
     5401 ttgaacagga tgccccgctt cgcgcccaaa ccggtcccga ccatgatcgc ggtaggcgtg
     5461 gccagcccca aggcgtcggg acaggtgatc acgaccaccg tgatcgcgta caggatcgcg
     5521 gtgctgaacg
//
LOCUS       DATBRK010000422         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_532755, whole genome shotgun sequence.
ACCESSION   DATBRK010000422 DATBRK010000000
VERSION     DATBRK010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532755"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VIH70_12495"
     CDS             complement(<1..222)
                     /locus_tag="VIH70_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8117227.1"
                     /translation="MPPSAWVLFAGTFVNRLGTFVLPFITLYLTSRHFSVAQAGLAVA
                     MYGIGGFAAQILGGLLTDRIGRRNTIGFSM"
     gene            complement(275..1012)
                     /locus_tag="VIH70_12500"
     CDS             complement(275..1012)
                     /locus_tag="VIH70_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8117228.1"
                     /translation="MAERTPRIEPVSEPARRAPAESSRAAPASERTRVRRLPERGVYD
                     RDAIDAIVDEALICHLAYVQDGEPRVIPTIHARVGDTLYVHGSNASRTLRSTKAGAPV
                     AAEVTLLDGLVLARSAFHHSMNYRSVVVYGRAREVTDPTEKFEAQRALVEHVVQGRSS
                     DARLPNDRELNQTTILAIPMDEASAKVRTGPPKDEDEDLELRVWAGVLPFTTVPGEPE
                     PDPLLPDGIDPPVYVSDYRRNGTSDRP"
     gene            complement(1061..1921)
                     /locus_tag="VIH70_12505"
     CDS             complement(1061..1921)
                     /locus_tag="VIH70_12505"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY8117229.1"
                     /translation="MPLYLLSEPTEPLVAPAIVAALDGWIDAAGASTAAAERLADGAA
                     PLATFDGDLLFDFRSRRPVLDVVDGRLSELTWPELTLRHRRLGSRDVLVLTGAEPDFR
                     WQELGRDVLSVVRRLGVTQWVSLGAIPTAVPHTRPVPVLATASDDGLLQEDEQVGPAG
                     LLRVPSAALSAIEKAVADDGVPTVGFYAQVPHYVGGPYAAAAIAILEHLGRHLGVEPP
                     LDGLPDEAMAQRERLDQIVQADDDSRTYVERLESVVDNQELPTGDELVGEIERFLQQR
                     GEGGSTGPMT"
BASE COUNT          277 a          715 c          737 g          277 t
ORIGIN      
        1 catcgagaac ccgatcgtgt tgcgccggcc gatccggtcc gtgagcaggc cgccgaggat
       61 ctgcgccgcg aagccaccga tcccgtacat cgccacggcg aggcccgcct gcgccacgga
      121 gaagtgccgc gatgtcaggt agagcgtgat gaacggaagg acgaaggtac cgagccggtt
      181 gacgaacgta ccggcgaaca gtacccacgc ggatggaggc aacgaccgca gattctcccg
      241 aagcgtgggt cgggggccgc ccatccgcgt cggcctacgg tcggtccgac gtcccgttac
      301 gccggtagtc gctcacgtac acggggggat cgatcccgtc cggcaggagc gggtcgggct
      361 cgggctcgcc ggggacggtc gtgaacggga ggacgccggc ccacacccgc agctcgaggt
      421 cctcgtcctc gtccttgggc ggcccggtgc gcaccttcgc cgacgcctcg tccatcggga
      481 tcgcgaggat cgtcgtctgg ttgagctcgc gatcgttcgg cagacgcgcg tcggacgagc
      541 ggccctggac gacgtgctcg acgagcgcgc gctgcgcctc gaacttctcg gtcgggtcgg
      601 tgacctcgcg cgcgcgtccg tagaccacga ccgatcggta gttcatcgag tggtggaagg
      661 cgctcctggc caggacgagc ccgtcgagca gcgtcacctc cgcggcgacc ggcgcgcccg
      721 ccttggtcga acggagcgtc cggctcgcgt tcgagccgtg gacgtagagg gtgtcgccga
      781 cccgggcgtg gatcgtcggg atcacccgcg gctcgccgtc ctgcacgtac gcgaggtggc
      841 agatcagggc ctcgtccacg atcgcgtcga tcgcgtcgcg gtcgtacacg ccgcgctcgg
      901 ggagccggcg gacgcgcgtg cgctcggagg cgggcgccgc gcgtgagctc tcggcgggcg
      961 cgcgccgtgc gggctcggaa accggttcga tccgtggggt ccgctccgcc atggcccgga
     1021 tggtaccggc cgcatcgacc ggcgtccgac tcgacggtgg ttacgtcatc ggaccggtgg
     1081 aacccccctc gccgcgttgc tggaggaagc gctcgatctc gcccacgagc tcgtcgccgg
     1141 tcggcagctc ctggttgtcc acgaccgact ccaggcgctc gacgtaggtc cttgagtcgt
     1201 cgtcggcctg gacgatctgg tccagtcgct ctcgctgcgc catcgcctcg tcggggagcc
     1261 cgtcgagcgg cggctcgacg ccgaggtgcc gcccgaggtg ttcgaggatc gcgatcgccg
     1321 cggccgcgta cgggccgccc acgtagtgcg gcacctgcgc gtagaagccg acggtcggca
     1381 cgccgtcgtc cgcgaccgcc ttctcgatcg ccgacaacgc ggcggagggg acccggagca
     1441 ggccggccgg cccgacctgc tcgtcctcct gcagcagccc gtcgtccgac gcggtggcca
     1501 gcacggggac cggacgcgtg tggggcacag ccgtcgggat cgcgccgagg ctcacccact
     1561 gcgtcacgcc gagccgccgc accaccgaga gcacgtcgcg cccgagctcc tgccaccgga
     1621 agtctggctc ggcgccggtg agcaccagga cgtcccggct cccgaggcgc cggtgacgca
     1681 gggtcagctc cggccaggtg agctcgctca gccggccgtc gaccacgtcg agcaccggcc
     1741 ttcgcgaacg gaagtcgaag agcaggtcgc cgtcgaacgt cgcgagcggc gccgcgccgt
     1801 cggcgagccg ctcggccgcc gcggtcgagg cccccgccgc gtcgatccag ccatcgaggg
     1861 ccgcgacgat cgcgggggcg acgagcggct cggtcggttc gctcagcagg tacagcggca
     1921 tggatcgagg atagccctcg gtcgcctcag ggacggacga gcagcccctg gaggccgcgg
     1981 atcacgtagt tcggcttcca gcgggg
//
LOCUS       DATBRK010000423         7913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1255617, whole genome shotgun sequence.
ACCESSION   DATBRK010000423 DATBRK010000000
VERSION     DATBRK010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255617"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..644)
                     /locus_tag="VIH70_12510"
     CDS             complement(303..644)
                     /locus_tag="VIH70_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117230.1"
                     /translation="MGKARTLLSCSACGQQLGQWVGRCPGCGAWGTVEQRNPGTASVP
                     VSLASLQHEARGEPRASTGIPGIDRVLGGGLVSATVALLAGEPGIAQFHPSYYEEPVM
                     RSRPPGTTKRV"
     gene            complement(691..1065)
                     /locus_tag="VIH70_12515"
     CDS             complement(691..1065)
                     /locus_tag="VIH70_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117231.1"
                     /translation="MTEGEVCWAVETSKDGERWRFFGKAWKKPGEPVLLHGVPRFVRF
                     RPVEEKRWREPLDRTGDHPMTLLNMEEGVRQELWPREEHVGLPVLLPGGEEGRLVSFK
                     HFDDGNRWDWVVEFRGMRQPHQ"
     gene            complement(1187..3667)
                     /locus_tag="VIH70_12520"
     CDS             complement(1187..3667)
                     /locus_tag="VIH70_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY8117232.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLNISLEAVRQQVEEIIGQGQAAPTGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLQKLGADLNRVRQTVIQLLSGYTGGKEQAPGEQAGQG
                     QGSMVLDQFGRNLTQLARELKLDPVIGREKEIERVMQVLSRRTKNNPVLIGEPGVGKT
                     AIVEGLAQDIVKGEVPETLKGKQIYTLDLGALVAGSRYRGDFEERLKKVLKEIRTRGD
                     IVLFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTRDEYRKHLEKDAALE
                     RRFQPIYVEEPTVAHSIEILKGLRDRYEAHHRVSYTDDALVAAANLSDRYISDRYLPD
                     KAIDLIDEAGSRMRIRRMTAPPNVREVDEKIAEVRLRKESAIDAQDFERAAALRDEEN
                     QLRQERERREREWKSGEMDILSEVDEEEIAEVLSSWTGIPVFKLTEEETEKLLRMEDE
                     LHKRIISQDDSIKAVSRAIRRTRAGLKDPKRPSGSFIFLGPSGVGKTELSKALAEFLF
                     GDEDALVQLDMSEYMEKHTVSRLIGSPPGYVGYEEGGQLTEAVRRKPFSVVLFDEIEK
                     AHPDVFNTLLQILEDGHLTDAQGHKVDFKNTIIIMTSNLGTRDIQKGPAIGFAARPDE
                     KLTYEKMKERVVEELKRSFRPEFLNRIDEVIVFHSLSREDVKNIVDLMMKRVREQLKS
                     KDLDIELTDAVKELLAEKGYDPALGARPLRRTIQRMVEDPLSEKLLYREFRAGQTVIV
                     DVQDGEITFEAPLSPETPPVELAGSSEA"
     gene            4082..4450
                     /locus_tag="VIH70_12525"
     CDS             4082..4450
                     /locus_tag="VIH70_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8117233.1"
                     /translation="MRVLVVDDHPAFRRALSSALRMVKGIEVAGEAGGGREATDRARK
                     DEPDLVLMDLSMPDFDGIEAMRRIHVDRPGLPVVILTAHADPGVEKEAREAGASGFLA
                     KGTALEDIVIVLHEASEGAA"
     gene            4565..6106
                     /locus_tag="VIH70_12530"
     CDS             4565..6106
                     /locus_tag="VIH70_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8117234.1"
                     /translation="MRRARTRAATTEDLPSTIAAEARIVGLRDFTTPSLEAVEHRRIQ
                     LWILTSIMLVSISIGVVVLSIWPISRGSAISSGPLRWGIVLLSVGFCVYAIEKERHLQ
                     KLSRLLTDERVLTAALSNRLRELSLLLQAGKAMNAVLELDAVLDVILHSAQELLAGAS
                     GSIMLVDGDSLVARCVRNNPDALGRRVAMDEGIAGRVARTMEPLLINGRPSSKEFPGL
                     DTRTLEVSSAMSVPIVHRERLLGVLNVNAEAGGTFSAYDLRALSLFAEQGAVAIANAR
                     LFDAERAHVVELVELDRMKSEFLGLVTHELRTPLTVVLAAAQTGKQPNSLIETSELFE
                     IIERNAKDLASLVEELLFAARLEQGEAAAEPVRVDLADLVRTVARDFGVTDRPVEVEV
                     DEGLSTMSDPDAIRRILVNLLDNAHKYGAPPIHVTLEPGEDCVVLSVSDAGPGLPVEE
                     RERLFERFHRADTTGKPGLGLGLPIVRGLAAACGGHVWAEDAPGGGAVFRVALPRETA
                     SLETV"
     gene            complement(6103..7632)
                     /gene="lysS"
                     /locus_tag="VIH70_12535"
     CDS             complement(6103..7632)
                     /gene="lysS"
                     /locus_tag="VIH70_12535"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY8117235.1"
                     /translation="MTDEASEEATGRERVVLRTRRESRDRLGERAFALSLEQALGVPE
                     PDTASSIRDEFGALEPDALDEARRTVAGRIVLKRDMGKLTFLVLRDRSGDIQLVVNQE
                     TDPESLALLSEVDLGDIVSATGRVGTTRKGELSIFVQRLAMLTKALRPLPEKWHGLRD
                     PELQQRQRYLHLATDPDARRRLVDARAQTLGAIRTYLDQRGYVEVETPVLQTVAGGAM
                     ARPFVTHHRDLDMDLYMRISLELHLKRLLVGGLERVYEIGRNFRNEGVGWKYNPEFTM
                     LELYRAYADYETMMDVTRDLVQASALAVVGSLEVSFRGHEIDLGGGWRRVTVLGSVSE
                     AVGEEVTLERSDLAQLAERHDVPIDPSWPGGKIALELFEKLVEPGLIQPTFVCDFPRD
                     VSPLARPHRDDPRLTEHVDPVIGGMELGTGYSELTDPDEQRAKFLLQLQAREAGELET
                     HPFDEDFLQALEHGMPPAGGLGIGIDRLLMLLTDAASIREVILFPLHRPPDRATGDPE
                     G"
     gene            complement(7625..>7913)
                     /locus_tag="VIH70_12540"
     CDS             complement(7625..>7913)
                     /locus_tag="VIH70_12540"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY8117236.1"
                     /translation="TARLPQVELQAPRSPIGRNTVDAIQSGLIFGTAAETDGMIERIR
                     KELGGSATVVATGGLAAQVVPHCQFVDEVDPWLTLEGLRLVFERNVGPADD"
BASE COUNT         1192 a         2745 c         2636 g         1340 t
ORIGIN      
        1 gggtcacacg ccaatcggca gggaagtgct cttagacgcg ggtccgggtg cccgagtcgg
       61 agtcgctatc aatagaactc taccgagccg ctattctcgg agtgcctcaa gaagccgata
      121 gctggttcac acatcgtcca gcaacgtcca aattgagctt atgtgaaccg aggagtgggg
      181 cgaagagctc ctcgatttga gctttcagat ggaccacggg tcccagtgtc gccagtgtgg
      241 aggagaaagg ttgcctccgg aggcctggcc cgctaatgaa atagtcggtt tgcagactgg
      301 agctagacgc gcttcgtcgt gcccggcggc cggctcctca taaccggctc ctcgtaatag
      361 gacgggtgaa actgggcgat gccgggctcg ccggccagca gggcgacggt cgcggaaacc
      421 agtccaccgc cgaggacccg gtcaatgccg gggatgccgg tggatgccct gggctcgccc
      481 ctcgcctcgt gctggagcga cgcgagcgag acgggaacgg aagccgtgcc ggggttccgt
      541 tgctcgaccg tcccccatgc gccgcagcca gggcagcgtc cgacccactg gcccagttgc
      601 tggccgcacg ccgagcacga caggagcgtc cgcgccttgc ccacgccggc acgctagcaa
      661 cggggtctga caggccacag cgaccgacac ctactgatgt ggctggcgca tcccccggaa
      721 ctcgacgacc cagtcccacc ggttgccgtc gtcgaaatgc ttgaacgaga ccaggcgccc
      781 ctcctcgccc cccgggagca gcaccggcag gcccacgtgt tcctcgcggg gccacagctc
      841 ctgcctcacg ccttcctcca tgttgagcaa cgtcatgggg tgatcgccgg tgcgatccag
      901 cggttcccgc caccgcttct cctcgaccgg ccggaaccgg acgaaccggg gcacgccgtg
      961 caggaggacc ggctcaccgg gcttcttcca ggccttgccg aagaaccgcc atcgctcgcc
     1021 gtccttcgac gtctcgacgg cccagcagac ctcgccttcc gtcacgggag tgaggctatc
     1081 cctccgcggg gacccgggga aaccgagagg cccagcaacc gtgtggttgc tgggccaacc
     1141 ggtcttgaag taaggcggcg ggagccgcct tacgtttctg accgccctac gcctccgagg
     1201 atccggccag ctcgaccggc ggggtctcgg gcgagagcgg agcctcgaac gtgatctcgc
     1261 cgtcctggac gtccacgatc accgtctgtc cggcccggaa ctcccggtac agcagcttct
     1321 ccgacagtgg gtcctcgacc atccgctgga tcgtccgccg gagcggtcgc gcgccgagcg
     1381 ccgggtcgta gcccttctct gcgagcagct ccttgaccgc gtcggtgagc tcgatgtcga
     1441 gatccttgga cttcagctgc tcacggaccc gcttcatcat caggtcgacg atgttcttca
     1501 cgtcctcgcg ggacagcgag tggaacacga tgacctcgtc gatgcggttg aggaactccg
     1561 gacggaagga acgcttgagc tcctccacga cccgctcctt catcttctcg taggtcaact
     1621 tctcgtcggg acgtgcggcg aacccgatgg ccgggccctt ctggatgtcc cgggttccca
     1681 ggttcgaggt catgatgatg atcgtgttct tgaagtccac cttgtggccc tgcgcgtccg
     1741 tgaggtgccc gtcttccagg atctggagca aggtgttgaa cacgtcggga tgcgccttct
     1801 cgatctcgtc gaacaggacg accgagaacg gctttcgccg gacggcctcc gtcagctggc
     1861 cgccctcctc gtaccccacg tagcctggcg gcgagccgat cagccgactg actgtgtgct
     1921 tctccatgta ctccgacatg tccagctgca ccagggcgtc ctcgtcgccg aacaggaact
     1981 cggccagcgc cttggaaagc tcggtcttcc cgacgcccga cggccccagg aagatgaacg
     2041 agcccgaggg gcgcttcggg tccttcagcc ccgcccgggt ccggcgtatg gccctggaca
     2101 cggccttgat cgagtcgtcc tggctgatga tccgcttgtg cagctcgtcc tccatgcgga
     2161 gcagcttctc ggtctcctcc tcggtgagct tgaacaccgg gatgccggtc caggacgaca
     2221 gaacctcggc gatctcctcc tcgtccactt cggacaggat gtccatctct ccggacttcc
     2281 actcgcgctc ccgccgctcc cgctcctggc gcagctggtt ctcctcgtcg cgaagcgccg
     2341 cggcgcgctc gaagtcctgg gcgtcgatgg ccgattcctt acgcaggcgg acctccgcga
     2401 tcttctcgtc gacctcgcga acgttcggtg gcgcggtcat ccgtcggatc cgcatgcgtg
     2461 agccggcctc gtcgatgagg tcgatggcct tgtccggcag atagcggtcg gagatgtacc
     2521 gatccgagag gttggccgcc gccacaagcg catcgtcggt gtacgacacg cggtgatgcg
     2581 cctcgtagcg atcccggagg cccttcagga tctcgatgga atgggcgacc gtcggctcct
     2641 cgacgtagat cggctggaac cgccgctcca gggccgcgtc cttctccaga tgcttgcggt
     2701 actcgtcgcg ggtggtggcc ccgatggtct ggagctcgcc ccgggcgagc atcggcttca
     2761 ggatgctggc cgcgtcgatc gcgccctcgg ccgcgccggc ccccaccagg gtgtgcagct
     2821 cgtcgatgaa cagcacgatg tccccgcggg tccgaatctc cttcaggacc ttcttcagcc
     2881 gctcctcgaa gtcgccgcgg tagcgcgatc cggcaaccaa cgccccgagg tcgagggtgt
     2941 agatctgctt gcccttcagg gtctcgggga cctcgccctt cacgatgtcc tgggcgaggc
     3001 cctcgacgat ggcggtcttg cccaccccgg gttcgccgat caggaccggg ttgttcttgg
     3061 tccgccggga caggacctgc atgacccgct cgatctcctt ctcccggccg atgacggggt
     3121 cgagcttcag ttcccgggcg agttgggtga ggttcctgcc gaactggtcc aggaccatcg
     3181 agccctggcc ctggcctgcc tgctcacccg gggcctgctc cttgccgccg gtgtatcccg
     3241 acaggagctg gatgaccgtc tggcggaccc ggttgaggtc cgcgccgagc ttctggagga
     3301 cctgggcggc cacgccctcg ccctcccgga tcaggccgag caggatgtgc tcggtcccga
     3361 tgtagttgtg tcccagctgc agggcttcgc ggagcgacag ctcgaggacc ttcttggccc
     3421 tcggggtgaa cgggatgtga cccgtcggcg cggcctggcc ctggccgatg atctcctcga
     3481 cctgctggcg gacggcctcc agagagatgt tcagagactc gagggccttg gccgcgaccc
     3541 cctccccctc gtggatcagg ccgagcagga tgtgctcggt cccgatgtaa ttgtggttga
     3601 gcatcctcgc ctcttcctgg gcgaggacca cgacgcggcg ggctcggtcc gtaaaacgct
     3661 cgaacatctc aatcccccgg ggccgaagcc ccacctatcg aacggcctcg ggaatcagcg
     3721 gaccccgtcc tacccccgag gcaggtcgag cgcttcggtg tggcacgatc tcgtgcgccc
     3781 gccgaagcgc cccacgcagg ggagtatacc cccgacccca cgtgcaaccg cccgtgcctc
     3841 ccgttcgttc ccatcgttcc cacgatcgtc ctcaacgatc tcttcggcca tatcccgtga
     3901 tcggatgagc gccgaagacg atgtcggggc cgatctaggc cccctgcctg ggccatagcc
     3961 cgaatgggct atacaagttc agccatttgg ggcgttgtcc ccgtcgggag tctcgggcag
     4021 gatgctgcca acggaaacct ctggtcttcc ctgaagatcc ttcctcggtg aggagaagac
     4081 ggtgcgggtg ttggtggttg atgaccaccc ggcgttccgc cgggccctct cgtccgcgtt
     4141 gaggatggtg aagggaatcg aggtcgccgg cgaggccggc gggggacggg aagcgaccga
     4201 tcgcgctcgc aaggacgaac ccgacctggt gctgatggac ctgtccatgc ccgacttcga
     4261 cgggatcgag gcgatgcgca ggatccatgt ggatcgaccc gggctacccg tcgtgatcct
     4321 gaccgcgcac gccgaccccg gcgtggagaa ggaggccagg gaggcggggg cctcggggtt
     4381 cctcgcgaag ggcaccgccc tggaagacat cgtcatcgtg ctccacgagg catccgaggg
     4441 cgccgcctag gcatcagggg tcggccgccc gaacgacgca ccccctgcgc gccgttgcgg
     4501 acaccagatc tcgcaacggc tcgcactttc aagggcgcct gaggcctgcc gatacacgtg
     4561 gtggatgaga cgagctcgaa cgcgcgccgc gacgaccgag gaccttccca gcacgattgc
     4621 cgccgaggcc cggatcgtcg gtctccgaga cttcaccacg ccctccctcg aggcggtcga
     4681 acaccgtcgg atccagctgt ggatcctcac atcgatcatg ctggtctcga tcagcatcgg
     4741 ggtcgtggtg ctgtcgatct ggcccatctc gcgtggttcg gcgatctcct ccggccccct
     4801 gcgatggggg atcgtcctgc tgtcggtggg cttctgcgtc tacgccatcg agaaggaacg
     4861 gcacctccag aagctgtcga ggctcctcac ggacgaacgg gtcctcaccg ccgccctctc
     4921 gaaccgcctt cgcgagctct cgcttctgct gcaggcgggc aaggcgatga acgcggtcct
     4981 cgagctcgac gccgtgttgg acgtgatcct gcacagcgcg caggagctgc tggccggggc
     5041 gagcggctcg atcatgctcg tggacggcga ttcgctcgtc gcccgctgcg tccgtaacaa
     5101 cccggatgcg ctgggccggc gcgtggccat ggacgagggg atcgccggcc gcgtggcgcg
     5161 aaccatggag ccgctcctga tcaacggacg accgagttcg aaggagttcc ccgggctcga
     5221 cacgcggacg ctggaggtct cgagcgcgat gtcggtcccg atcgtgcatc gcgaacggct
     5281 cctcggcgtg ctcaacgtga acgcggaggc gggcggcacg ttcagcgcgt acgaccttcg
     5341 cgcactgagc ctgttcgccg agcagggcgc ggtggcgatc gcgaacgcga ggctcttcga
     5401 cgccgagcgc gcccacgtcg tcgagctcgt cgagctcgac cggatgaagt ccgagttcct
     5461 cgggctcgtc acccacgagc tccgaacgcc gctcacggtt gtgctcgcgg ccgcgcagac
     5521 cggcaagcag ccgaactcgt tgatcgagac gtccgagctg ttcgagatca tcgaacgcaa
     5581 cgcgaaggac ctcgcatcgc tcgtcgagga gctgctgttc gccgcgcggc tcgagcaggg
     5641 cgaggcggcg gccgaaccgg tccgcgtcga tctcgccgat ctcgttcgaa cggtcgcgcg
     5701 cgacttcggc gtcacggatc gaccggtgga ggtcgaggtc gacgaggggc tctcgacgat
     5761 gagcgacccc gacgcgatcc ggcggatcct ggtgaacctg ctggacaacg cccacaagta
     5821 cggggcccca cccatccacg tgacgctcga gccgggcgag gactgcgtgg tcctcagcgt
     5881 ctccgacgcc ggtccgggcc tgcccgtcga ggaacgcgag cggctgttcg agcggttcca
     5941 ccgcgccgat acgaccggca agccgggcct cgggctgggc ctgccgatcg tccgcgggtt
     6001 ggccgccgcg tgcgggggcc acgtctgggc ggaggacgcg ccgggcggcg gtgccgtctt
     6061 ccgggtcgca ctgccgcgcg agacagccag cctcgaaacc gtctagccct ccgggtctcc
     6121 cgtcgcccgg tccggcgggc ggtgcagcgg gaacaggatg acctcacgga tgctcgccgc
     6181 gtcggtgagc agcatgagga gccggtcgat cccgatcccg agccccccgg caggcggcat
     6241 cccgtgttcg agcgcctgga ggaagtcctc gtcgaacgga tgggtctcca gctcgccggc
     6301 ctcgcgcgcc tgcagctgga gcaggaactt cgcgcgctgc tcgtcggggt cggtgagctc
     6361 cgagtacccc gtgccgagct ccatcccgcc gatcaccggg tcgacgtgtt cggtgaggcg
     6421 ggggtcgtcg cggtggggcc gcgccagcgg tgacacgtcg cgcgggaagt cgcagacgaa
     6481 cgtgggctgg atcaggccgg gctcgacgag cttctcgaac agctcgagcg cgatcttgcc
     6541 cccgggccag gacgggtcga tggggacgtc gtgccgctcc gcgagctggg cgaggtccga
     6601 gcgctccagc gtgacctcct ccccgacggc ctccgagacg ctccccagga ccgtcacgcg
     6661 gcgccacccc ccgccgagat cgatctcgtg cccacggaac gagacctcga gcgagccgac
     6721 taccgcgagc gcgctcgcct gcacgagatc gcgcgtgacg tccatcatcg tctcgtagtc
     6781 ggcgtaggcc cgatagagct cgagcatcgt gaactcgggg ttgtatttcc accccacgcc
     6841 ctcgttgcgg aagttccgcc cgatctcgta cacgcgctcg aggccgccca ccagcagccg
     6901 cttcaggtgc agctcgagcg agatccgcat gtacagatcc atgtcgaggt ctcggtggtg
     6961 cgtgacgaag ggcctggcca tcgccccccc cgcgaccgtc tgcaggaccg gcgtctcgac
     7021 ctcgacgtac ccgcgctggt ccaggtaggt tcggatcgcg ccaagggtct gggcacgcgc
     7081 gtcgaccagc cgcctcctcg cgtcggggtc ggtcgcgagg tgcagatagc gttgtcgctg
     7141 ctggagctcg gggtccctga ggccgtgcca cttctcgggc agggggcgga gcgccttggt
     7201 gagcatcgcg agccgctgga cgaagatcga cagctcgccc ttgcgcgtgg tcccgaccct
     7261 gccggtcgcg ctcacgatgt cgccgagatc gacctccgac agcagcgcga ggctctccgg
     7321 gtcggtctcc tggttgacga cgagctggat gtcaccggac cggtcgcgca ggaccaggaa
     7381 cgtgagcttg cccatgtcgc gcttcagcac gatgcgaccg gccaccgtcc ggcgagcctc
     7441 gtcgagcgcg tccggctcga gcgcgccgaa ctcgtcccgg atgctcgagg ccgtgtccgg
     7501 ttcgggcacg ccgagcgcct gctcgagact cagggcgaac gcacgctcgc cgagccgatc
     7561 gcggctctcg cgtcgcgtcc gaaggaccac gcgttcgcgc ccggtggcct cctcggaggc
     7621 ctcgtcagtc atcggcgggc ccgacgttgc gctcgaagac gagacggagg ccttcgagcg
     7681 tgagccacgg gtcgacctcg tcgacgaatt ggcagtgcgg gaccacctgc gcggccaggc
     7741 ctccggtcgc gacgaccgtg gcgctgccgc ctagctcctt gcggatgcgc tcgatcatcc
     7801 cgtcggtctc ggccgcggtc ccgaagatca gaccggactg gatcgcgtcg acggtgttcc
     7861 gcccgatcgg cgacctcggc gcctggagct cgacctgtgg cagccgcgcc gtc
//
LOCUS       DATBRK010000424         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1401310, whole genome shotgun sequence.
ACCESSION   DATBRK010000424 DATBRK010000000
VERSION     DATBRK010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401310"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VIH70_12545"
     CDS             <1..443
                     /locus_tag="VIH70_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006346451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117237.1"
                     /translation="LANIALSVLDEHFAEAWQQTMGTNAQRRTRKARGLARYRLVRYA
                     DDCAPRTLKEVRCRRRYRRMRCCTRDEGAGRLGWWPFAVALQGEAANHREVRRSRAGV
                     LSLRRWRAVSASGVDHEGERKRTTDEASKAYGWHRNRGRVVAPR"
     gene            762..>1704
                     /gene="ltrA"
                     /locus_tag="VIH70_12550"
     CDS             762..>1704
                     /gene="ltrA"
                     /locus_tag="VIH70_12550"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEY8117238.1"
                     /translation="MSEPKPKSFEISKWAVWEAYRRVKANKGAAGVDEQSIAEFEQDL
                     YGNLYKLWNRLSSGSYFPPPVRAVEIPKRDGSSRTLGVPTVADRIAQTVVRSYLEPLV
                     EPVFHEDSYGYRPGRSAHDAIGVCRGRCWWNDWVLDMDIRKFFDSVPWDLTLKAVAHH
                     TDQRWILLYVERWLKAPLQTEDGRLVKRDRGTPQGSAISPLLANMFLHYAFDAWMARE
                     FPTVQFERYCDDIIVHARTEGHARFLWAAIAKRLAECGLQLNERKTRIVYCKDDHRRG
                     SYEHASFDFLGYTFRPRLSKSKLGKHFLNFSPAVSGEA"
BASE COUNT          303 a          484 c          631 g          286 t
ORIGIN      
        1 tgctggccaa catcgccctg tcggtcctcg acgagcactt cgccgaggca tggcagcaga
       61 cgatgggcac caacgcccag cgccgcacgc gcaaagccag gggactggcc cgctatcgcc
      121 tggtccgcta cgcggacgac tgtgcgccga gaacgctgaa ggaggttcga tgtagaaggc
      181 gataccgacg gatgcggtgc tgcacgagag atgaaggagc cggccgattg ggctggtggc
      241 ccttcgcggt cgccctgcag ggggaggccg caaaccaccg cgaggtgcgc cggtcaagag
      301 ccggggtgct gagcttgcgg agatggcgcg ctgtcagcgc gtcaggtgtg gatcacgaag
      361 gcgagcgcaa gcgaaccact gatgaggcgt cgaaagcgta tggatggcat cgaaaccggg
      421 gtcgagttgt tgccccgcga tgagcctggc gggtgcctgc tgactggcca ggtggtgtcc
      481 ggcgtgaagg tggcgcgagc gtggtccggg ctctcgtgcg gaactcggga acctgtcgcc
      541 ccgcgcccgc gggcggccag tggcgcaagt tctggcctgc ggctgctggt ggaggcgaga
      601 accccgagca gcgggaactg cgagggggag agtagtgatg cggggcacag gggcggaccg
      661 tctcgtagta gcgacgaagg ccctgtaatg gggctggagc gaaggggacg ggtcgtcctg
      721 gtgagcttgg cggtcaacca cctgcgggcg ggaggagccg ggtgagcgag ccaaagccga
      781 agtcgtttga gatctccaag tgggccgttt gggaggccta tcggagggtc aaggcgaaca
      841 agggcgcggc cggcgtggac gagcagtcga tcgcggagtt cgagcaggac ctttacggga
      901 acctgtacaa gctctggaac cggctctcgt cggggagcta tttcccgccg ccggtgcgcg
      961 cggtggaaat accgaagcga gacggcagct ctagaacgct aggggtgccg accgtcgcgg
     1021 ataggatcgc ccagaccgtc gtgcgctcat acctggagcc gttggtggag ccggtgttcc
     1081 acgaggactc ctatgggtat cggccggggc ggtcggcgca tgacgcgatt ggggtctgtc
     1141 gcgggcgctg ctggtggaac gactgggtgc tcgatatgga catccggaag ttcttcgata
     1201 gtgtcccgtg ggacctcacg ctcaaggcgg tagcccacca caccgaccag cgctggatcc
     1261 tgctgtacgt ggagcggtgg ctcaaagcgc cgcttcagac cgaggacggc aggctggtca
     1321 agcgggatcg cgggaccccg caggggtcgg cgatttcacc gttgctcgcc aacatgttct
     1381 tgcactatgc gttcgatgcg tggatggctc gggaattccc gaccgtccag ttcgagcgtt
     1441 attgcgatga catcattgtt cacgcccgaa ccgaaggtca cgcccgcttt ctgtgggcgg
     1501 cgatcgcgaa acggttggcg gagtgcggac ttcagctcaa cgagcgtaag acccgcatcg
     1561 tgtactgcaa ggacgatcac cggcgcggct catacgagca cgcgtcgttt gacttcctcg
     1621 ggtacacgtt ccgcccccgg ctgtcgaaga gcaagctcgg gaagcacttc ctgaacttct
     1681 ctccggcagt gtcgggcgag gcga
//
LOCUS       DATBRK010000425        11859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_684487, whole genome shotgun sequence.
ACCESSION   DATBRK010000425 DATBRK010000000
VERSION     DATBRK010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11859
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684487"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1028
                     /locus_tag="VIH70_12555"
     CDS             228..1028
                     /locus_tag="VIH70_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY8117239.1"
                     /translation="MPDDRPPIICSTISVFSKPIDAAFPLFAQIGFDGIELMVTNDPD
                     TQDASRVRALSEDHALPVLAIHAPFLLMSRRVWGRDPVGKIDRAIELALGVGAPLVVV
                     HPPYRWQSAYRRWLDERLPAEAIDRGVRVAVENMFPLRVGGRSVARFHAPQSLDELDG
                     FPDVTLDTSHLAVSGVDPVEAAASLGDRLAHVHLSNNAGKGWDSHLPLDQGVLDLGRF
                     LEALAARGFRGAISLEIDLRGHLDDDQALRRILSRNRELCEAGLTLPA"
     gene            1058..1210
                     /locus_tag="VIH70_12560"
     CDS             1058..1210
                     /locus_tag="VIH70_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117240.1"
                     /translation="MRRFTLITIVVLFALIVGAAVYQIALANHDQAPFPGPVEGTPFP
                     SLTPPP"
     gene            complement(1215..1964)
                     /locus_tag="VIH70_12565"
     CDS             complement(1215..1964)
                     /locus_tag="VIH70_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8117241.1"
                     /translation="MRAAVPVRARPIAFLLLGAIALAACGEPPPPDLSGSRAVTFESR
                     DGVTLEGRLFGDGSTGVVLSHMRPADQRSWFAFANRLADEGYLVLTYDFRGYCPGGEG
                     GCSQGDLQISAIWQDVLGAVDFVRSQGATTVALVGASMGGTASLVAAGEEGTDVAVVV
                     TLSAPTSIEGLDADAALLQRVDANKLFIAGVGDAAAAASAEQLYEIAPPPKAAPEIVP
                     SDDHGTDLLTGSRGEAVQRVIETYLASFAPA"
     gene            complement(1976..2077)
                     /locus_tag="VIH70_12570"
     CDS             complement(1976..2077)
                     /locus_tag="VIH70_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007465623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEY8117242.1"
                     /translation="MPSLVKKRRKKMRKKKHKKLLKKTRWQRRQQGR"
     gene            complement(2089..2979)
                     /locus_tag="VIH70_12575"
     CDS             complement(2089..2979)
                     /locus_tag="VIH70_12575"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HEY8117243.1"
                     /translation="MNEHEALVDQAVRTKHTVLLIGGMDTGKTSLSRELLAAALAAGR
                     PAALLDADVGQKTVGPPATISLKIVRSPEDLEPEPLATPEALYFVGSTSAQGHLLPVV
                     GGVAALHEQAKQLGADFIVVDSSGLVSGITGQTLKYHKVEALRPDLVVGLQRGEELLP
                     LLGVIQRFFATEVVPLGVHPRVVPTTVDLRAENRERSMRRYFSGELHRFRVKPTVFMP
                     ALPPLFEPADLDRLLVGLSDGAGAYPGIGYLEHAPGDGVIRLISPVAEGPKALRLGSV
                     RLDASFRAKRVDLRNLFGSE"
     gene            complement(3219..4046)
                     /locus_tag="VIH70_12580"
     CDS             complement(3219..4046)
                     /locus_tag="VIH70_12580"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEY8117244.1"
                     /translation="MDVIRSHAARLPALDDRWTPGAVGREELLEGLRSGAVAGPEISH
                     PLDNVLRNILLLHEGDPDKQFGMTGLQTLEPDEILELVGRAAGFEPDPNATTGPVPVD
                     PERVLDACGDAGDRFAEAILRRERVILATGHPVGLAHLYIEVGRLLRAGGATIVEPAD
                     GVTWREVDRPHPWQIRYLDGVAILTDKASARHTHSGDAMERMLDDLTPDLVFADHGFA
                     GAAIERGVETVSIADVNDPALLVARAQGRTRTVIVMDDNVAPQAYWPCFQAIAARLP"
     gene            4096..4884
                     /locus_tag="VIH70_12585"
     CDS             4096..4884
                     /locus_tag="VIH70_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8117245.1"
                     /translation="MELRGSVVVVTGASSGFGELAALRFGRAGARVVLAARRLERLDA
                     LAERIASQGGLALAVRCDVTELEQLTALRDRVGTTFERCDVLVNNAGIRGGGPFETTD
                     IECLNRVIDVNLRGVVLGTKLFLPMMLERGRGHIVNVASLAGRFATPGAAIYGATKHA
                     VVAFSEALYHELGPKGVLVTSVNPGFSPTEGFPATGPRLVRLDPDDVAKLIVDVVRKG
                     TAPEISIPRALSALQAFRVLTPPLYRWGLGTISRHHGSSGSAEG"
     gene            complement(4972..5148)
                     /locus_tag="VIH70_12590"
     CDS             complement(4972..5148)
                     /locus_tag="VIH70_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117246.1"
                     /translation="MSEFGRSGPQLPHDEASQKEHLKEVEEDLHHEHEAERVAKAHPK
                     RERPWWRFWAKASG"
     gene            complement(5167..6315)
                     /locus_tag="VIH70_12595"
     CDS             complement(5167..6315)
                     /locus_tag="VIH70_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEY8117247.1"
                     /translation="MSGIAGLVLCPEARIYDHGPQHPLRPQRVLYTWSLIQACGLLER
                     PNVSRLTCRSADDAEIGLVHSGAYIEATRKAGHGAAGDWGRYGFGPGDNPIFPNMHEA
                     GALVAGASLVAAEAVRDGSVLHAFNAAGGLHHAMAARASGFCVYDDPAVAIAWLLDHG
                     VHPIAYVDVDVHHGDGVQAIFYEDPRVLTVSLHQSGATLFPGTGFVDERGAGAAEGTK
                     VNVPLEPGVGDRAWLAAFAEIVPPAVRAFGPKVLVTQLGCDTHRTDPLAQLRLSTAAY
                     RDTAAALHALAHEVTGGRWLATGGGGYQWATVVPRAWTLAFAEMSDAELPDALPEPWI
                     ERVELEIGGEVPATFSEPPLEHDALDGGTERTIERVKRTCFPYFGLRT"
     gene            complement(6312..6494)
                     /locus_tag="VIH70_12600"
     CDS             complement(6312..6494)
                     /locus_tag="VIH70_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117248.1"
                     /translation="MDTEGSRGEGDLPDLRGEPEPDWVEDIRRGRKERAKRLKSLLGQ
                     APPADAAEGEEPPRTA"
     gene            complement(6497..7267)
                     /locus_tag="VIH70_12605"
     CDS             complement(6497..7267)
                     /locus_tag="VIH70_12605"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007981305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8117249.1"
                     /translation="MSEDGYTKNEMMIAASARTLAGERVCFVGVGLPNIVCNLAQRTV
                     SPELQLVYEAGVFGARPERLPLSIGDPTLVSGSMAVTSMFELFAFYLQAGLIDAAFLG
                     GAQIDRFGNLNTTVIGDYAAPKVRLPGSGGACEIAIHARRILVIMRQAKRSFVADLDF
                     RTSPGHSGDPAHDRARGWFGSGPTSVVTDLATFGFDEETGEMTLLTVHPGVSIDDVRA
                     NMGWDPRISGSLGETPSPTEDELRLIRTQLDPQGMYRS"
     gene            complement(7284..8180)
                     /locus_tag="VIH70_12610"
     CDS             complement(7284..8180)
                     /locus_tag="VIH70_12610"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020634725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8117250.1"
                     /translation="MADAVGELVRDGDTVAIEGFTHLICFAAGHEIIRQRKRDLTLAR
                     MTPDVIYDQMIGAGVARKLIFSWLGNPGVGSLHAVRRRIEQADPEPLEIEEYSHFGMV
                     CRYVAGASNLPFFPIRSYYESDIPSVNPNIKPMRSPYQDGTEVYVVPPLRPDVTIVHA
                     QRATATGDTQIWGLLGCQKEAAFAAERVIVVCEEIVDEAVVRRDPNRTVIPGVVVDAV
                     VEQPFACHPSYAQGYYDRDNAFYLEWEGISKDPEALERWLKEWVHGLQGHAEYVARLG
                     DRWEGLRAGERLSGEVNYGDYS"
     gene            complement(8231..8605)
                     /locus_tag="VIH70_12615"
     CDS             complement(8231..8605)
                     /locus_tag="VIH70_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8117251.1"
                     /translation="MALSVATLTWDCRSPEVVARFWAGVLGYEVVEIDDEDALIRDPR
                     GKGWPILFQSVPESKAVKNRLHLDLRPATSMADEKARVSELGASEFRYVNEGGSFWTV
                     MLDPEGNEFCVLRGPEDGWSPE"
     gene            complement(8620..9906)
                     /locus_tag="VIH70_12620"
     CDS             complement(8620..9906)
                     /locus_tag="VIH70_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8117252.1"
                     /translation="MSTAETVDLAEAQAPRLVTEIPGPEARARIERDRKVTSPSLPRA
                     YDLVPSRGAGSVIEDVDGNRFLDVNAGIAVTSTGHAHPKVVEAIQLQAEQLLHYSASD
                     FYLPIYSDVCEELDRIAPFSGGARSFLTNSGTEAVEGAIKLARYATGRQYVVAMIGSF
                     HGRSYGSVSLTASKAKYHAGFGPLLPGVVHTHFGDFEHLERVVFARLIPPDEVAAIVV
                     EPVLGEGGYVFPPEGWFAYLRELCDRHGILLVADEVQSGMGRTGKMWAIEHESIEPDV
                     LLAGKGIASGMPLGAIVARDRLMTWDVGAHGSTFGGNPVACAAALATLELIEGVLLDN
                     ARDVGELLVKGLRDIQARRPEIVDVRGRGLMIGIEFADGQTMHAVEQAAFRRGLLVLG
                     AGDSVIRMSPPLVFRPDQAETALQVFDDAVADVAGG"
     gene            complement(9903..11468)
                     /locus_tag="VIH70_12625"
     CDS             complement(9903..11468)
                     /locus_tag="VIH70_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8117253.1"
                     /translation="MAEEGFRITYATMAADNEDMHEAYDRGVEVARSWLGQRHPLFVN
                     GEARGGSGYKAAHSPIDGTLIGEFASGTREDVRDAVAAAKGAFHDWADRPWQERVAII
                     RKAADIMSDQRNELSALMAMEVGKNRLEALGDVEESADLLRWNANEVEQNEGFRKPMQ
                     SLGSPGEYYDVLRPYGVWAVISPFNFPMALAAGPSSAALVAGNTVVFKPAHMGVFTGL
                     KLYEVYMAAGVPKGVFHFLSGPGSVVGDEIVNHPDVAGITFTGSYEVGMGIYKGFAKD
                     FPKPVICEMGGKNPTIVTDKADLDKATDGVLRSAFGFGGQKCSANSRVYVHRSVYDEF
                     VRQLRKKTEDVKIGDPLDRDVYLGPVIDESAVTTFEEASNEAKKNGTVVTGGERLTEG
                     ELAKGTFVQPTIAEVPLDSWIWKKELFVPFVAVAPYDDLDEAIRLSNDTEYGLTAGFY
                     SEDRAEIDRWLSSIEAGVVYVNRRAGATTGAWPGVQPFGGWKGSGTSGKAGGGPYYVQ
                     QYLREQSRTVIEE"
     gene            11624..>11859
                     /locus_tag="VIH70_12630"
     CDS             11624..>11859
                     /locus_tag="VIH70_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117254.1"
                     /translation="MRLVTFDRRGHRRLGALREGNVVDLPSLLGHPAFPETMERLVRS
                     NGGTVLDAARAALERDEADDFVVERPRLLAALLPT"
BASE COUNT         1705 a         4304 c         4044 g         1806 t
ORIGIN      
        1 tcccccgagg tccatccgtt cgcgacgccg aacgcgacgg cgagggacgc ccccgcgtac
       61 atcaggagcg cgagcgccca gaccagctcg tacggccgac ggctgccggc gtaccggcgc
      121 acgagcgccg cgccgaacag cacggcgacg agcgccgccg cgagggggaa cacccacatg
      181 ggccgcagca tagccgtggc ggggaggacc gctgttagca tcgcggcgtg cccgacgatc
      241 gtccccccat catctgctcg acgatctcgg tcttctccaa gccgatcgat gcggcgttcc
      301 cgttgttcgc gcagatcggc ttcgacggga tcgagctgat ggtcacgaac gatcccgaca
      361 cccaggatgc gtcgcgcgtt cgagcgctct ccgaggacca cgcgctcccg gttctggcga
      421 tccacgcccc gttcctcctg atgtcccgaa gggtctgggg ccgcgacccc gtcggcaaga
      481 tcgaccgagc gatcgagctc gcgctcggcg tgggtgcgcc gctcgtcgtg gtgcacccgc
      541 cctatcggtg gcaatcggcc tatcggcgct ggctcgacga acggctcccc gcggaagcga
      601 tcgaccgcgg cgtgcgggtc gccgtcgaga acatgttccc gttgcgcgtc gggggccgga
      661 gcgtggctcg gttccacgcg ccgcagtcgc tcgacgagct cgatggcttc ccggacgtga
      721 cgctggacac gagccacctc gcggtctcgg gggtcgaccc cgtcgaggcg gcggccagtc
      781 tcggcgaccg gctggcccat gtccacctgt cgaacaacgc gggcaagggc tgggactcgc
      841 acctgcccct ggaccagggc gtcctggacc taggccggtt cctcgaggcg ctcgcggcgc
      901 gcgggttccg cggagcgatc tcgctcgaga tcgacctccg cggccacctc gacgacgacc
      961 aggccttgcg ccgcatcctt tcccgtaacc gcgagctctg cgaggcgggg ctcacgctcc
     1021 ccgcctgacc cggacccgcc actgctagcc tccggcgatg cgccgcttca cgttgatcac
     1081 gatcgtcgtg ctgttcgcgt tgatcgtcgg cgcggccgtc taccagatcg cgctcgcgaa
     1141 ccacgaccag gccccgttcc ccggtccggt cgagggcacg ccgttcccga gcctcacccc
     1201 gcccccgtga agtttcacgc cggcgcgaac gacgcgaggt aggtctcgat gacgcgttgg
     1261 accgcttctc cgcgcgagcc cgtcagcagg tcggtgccgt ggtcgtcgga ggggacgatc
     1321 tcgggggccg ctttcggcgg cggggcgatc tcgtagagct gctcggccga tgccgcggcg
     1381 gccgcgtccc cgacacccgc gatgaacagc ttgtttgcgt ccacgcgttg gagcagcgcg
     1441 gcgtcggcgt cgagcccctc gatcgatgtc ggtgccgaga gggtcaccac cacggccacg
     1501 tccgtcccct cctccccggc cgcgaccagc gaagccgtgc cgcccatgct cgcgccaacc
     1561 agcgcgacgg tcgtcgcccc ctgggaccgg acgaagtcca ccgcgcccag gacgtcctgc
     1621 cagatcgccg agatctggag atctccctgc gagcagccgc cctcgccgcc ggggcagtac
     1681 ccgcggaagt cgtacgtgag gacgaggtag ccctcgtcgg cgagtcggtt cgcgaacgcg
     1741 aaccacgagc gctgatcggc gggtcgcatg tgcgacagca cgacgccggt cgagccgtcg
     1801 ccgaacagac gcccctccag cgtcacgccg tcgcgggact cgaacgtgac cgcccgcgaa
     1861 cccgagagat cgggaggcgg cggttcgccg cacgccgcga gcgcgatcgc tcccagcagc
     1921 aggaacgcga tcggacgggc ccggaccggg accgccgcgc gcaccgacgc gtctcctagc
     1981 ggccttgctg acggcgctgc cagcgggtct tcttcagcag cttcttgtgc ttcttcttcc
     2041 gcatcttctt gcggcgcttc ttgaccaagg acggcacgca gcgaactcct attccgatcc
     2101 gaacaggttc cggaggtcca cgcgcttggc gcggaaggac gcatccagac ggaccgaccc
     2161 cagtctaagc gccttgggac cctctgccac gggcgagatg agccggatca caccgtcgcc
     2221 cggcgcgtgc tcgaggtagc cgatccccgg gtaggcgccg gcgccgtcgg acaggccgac
     2281 caggagccgg tcgaggtccg cgggttcgaa cagcggtggg agcgccggca tgaacacggt
     2341 gggcttgacc cggaagcgat ggagctcgcc cgagaagtac cggcgcatcg atcgctctcg
     2401 gttctcggcc cgcaggtcca ccgtcgtcgg gacgaccctc ggatggaccc cgagcggcac
     2461 cacctcggtc gcgaagaacc gctggatcac accgagcagc ggcagcagct cctccccacg
     2521 ttgcagcccc acgaccaggt cgggccggag cgcttccacc ttgtggtact tgagcgtctg
     2581 cccggtgatc ccggacacga gcccggacga gtcgacgacg atgaaatcgg ccccgagctg
     2641 cttggcctgt tcgtggaggg cggcgacccc cccgaccacc ggcaggaggt ggccctgcgc
     2701 cgaggtcgag ccgacgaagt atagcgcctc cggcgtcgcg agcggttcgg gttcgaggtc
     2761 ctccgggctc cgtacgatct tgagcgagat cgtcgccggt ggccccacgg tcttctgccc
     2821 gacatcggcg tccaggagcg ccgcgggccg gcccgcggcg agcgcagccg ccaggagctc
     2881 gcgcgacagg ctggtcttcc cggtatccat gccgccgatc aggagcaccg tgtgcttggt
     2941 tcgcaccgcc tggtcgacga gcgcctcatg ctcgttcaca tccgcctccg ggttctccca
     3001 cccggaggcc ggtcgcacgc gtgcgtgcgt ctatcccgcc ggggagaccc cccgcccgga
     3061 cagccgtctc tcggtggtct ggatggtcgt catggagccc gcggctccgc tcacgcgttc
     3121 gaaccgtgcc tctggagtgg gtgtgatcgt caagatggcc ttcatcctat ccggctcagc
     3181 gctcgaacgc gcgcgccggg gtctcgacgc aggggagatc aggggaggcg ggcggcgatc
     3241 gcctggaagc agggccagta ggcctgcggg gccacgttgt cgtccatcac gatcactgtg
     3301 cgggtcctgc cctgggcccg ggcgacgagg agggcggggt cgttcacgtc ggcgatcgac
     3361 accgtctcga cgccgcgttc gatcgccgcc cccgcgaacc cgtggtcggc gaacaccaga
     3421 tcgggggtca ggtcgtccag catgcgctcc atcgcgtccc cgctgtgggt gtgccgcgcg
     3481 ctggccttgt ccgtcaggat cgcgacgccg tccaggtacc ggatctgcca cggatggggg
     3541 cggtcgacct cgcgccacgt gaccccgtcg gcgggttcga cgatcgtcgc gccgccggcg
     3601 cggagcagcc gcccgacctc gatgtacagg tgcgcgagtc ccaccgggtg ccccgtcgcg
     3661 aggatcacgc gttcgcgtcg caggatcgcc tcggcgaacc ggtcgccggc gtcgccgcac
     3721 gcgtccagga cgcgttcggg gtcgaccggg acggggcccg tggtggcgtt cggatccggc
     3781 tcgaacccgg cggcccggcc cacgagctcg aggatctcgt ccggctcgag cgtctggagc
     3841 ccggtcatcc cgaactgctt gtccgggtcg ccctcgtgga gcagcaggat gttgcgcagc
     3901 acgttgtcga gcggatggct gatctcgggc ccggccacgg cgcccgaacg caggccctcg
     3961 agcagctcct cgcgcccgac cgcgccgggt gtccagcggt cgtccagggc cggcaggcga
     4021 gcggcgtgcg atcggataac gtccatcgat cccatcgtac gaggagcgcg gcgatgaccc
     4081 gagaggaggc ctgaggtgga gctccgaggt tcggtcgtgg tggtcaccgg ggcgtcgtcg
     4141 ggcttcggcg agctcgccgc gctccggttc ggccgcgcgg gcgcccgagt cgtgctcgcc
     4201 gcgcgacggc tcgaacgcct cgacgcgctc gccgaacgga tcgcctcgca gggcggcttg
     4261 gccctcgcgg tccgatgcga cgtgacggag ctcgagcagc tcaccgcgct gcgcgaccgc
     4321 gtcgggacga cgttcgaacg ctgcgacgtc ctggtcaaca acgcgggcat ccgcggcggc
     4381 gggccgttcg agaccacgga catcgagtgc ctcaaccgcg tgatcgacgt gaacctgcgg
     4441 ggcgtcgtcc tcggcaccaa gctcttcctc ccgatgatgc tcgaacgcgg gcgcggtcac
     4501 atcgtgaacg tcgcgtcgct cgccggacgg ttcgcgacgc ccggggccgc gatctacggc
     4561 gcgaccaagc atgccgtggt cgcgttcagc gaggcgctgt accacgagct cggcccgaag
     4621 ggggtcctgg tcacgagcgt gaacccgggc ttctctccga cggaggggtt cccggcgacc
     4681 ggtccgaggc tcgtccggct ggacccggac gacgtcgcga agctgatcgt cgacgtcgtc
     4741 cgcaagggaa cggcgccgga gatctcgatc ccccgggcgc tctccgcgct ccaggcattc
     4801 cgcgtcctga ccccgcccct gtaccgctgg ggcctgggca ccatctcccg gcaccacggg
     4861 tcgagcggct ccgccgaggg ctgagcgcac gtagagcggg gccgagacca gggcgcgacg
     4921 catcgagctg acgatccagc aactggtcga gcggccgtcg ggagactcgg cctagccgga
     4981 agccttcgcc cagaacctcc accacgggcg ctccctcttc gggtgcgcct tcgcgacgcg
     5041 ctcggcctca tgctcgtggt ggaggtcctc ctcgacctcc ttcaggtgct ccttctgaga
     5101 cgcctcgtcg tgcgggagct gtggcccgct ccgtccgaac tcgctcatgc gagaaggata
     5161 ggcgcctcac gtacgcagtc cgaagtaggg gaagcacgtg cgcttgaccc gctcgatcgt
     5221 ccgctcggtc ccgccgtcga gcgcgtcgtg ttcgaggggc ggctcggaga acgtggcggg
     5281 gacctcgcca ccgatctcga gctcgacccg ctcgatccac ggctcgggca gggcgtcggg
     5341 gagctcggcg tcggacatct cggcgaacgc gagcgtccag gcgcgcggga cgacggtcgc
     5401 ccactggtag ccaccgccgc cggtcgcgag ccatcgtcct ccggtcacct cgtgcgcgag
     5461 cgcatggagc gccgccgcgg tgtcccgata cgcggcggtc gagagccgga gctgggccag
     5521 cgggtcggtc cgatgcgtgt cgcacccgag ctgcgtgacg agcaccttcg gcccgaacgc
     5581 ccggacggcg ggcggcacga tctcggcaaa ggccgcgagc caggcgcggt cgccgacccc
     5641 cggctcgaga ggcacgttga ccttggttcc ctcggcggcg cccgcgccgc gttcgtcgac
     5701 gaagcccgta cccgggaaca gcgtcgcgcc cgattgatgg agcgagacgg tcagcacccg
     5761 cggatcctcg tagaagatcg cctggacgcc gtcgccgtga tggacgtcga cgtccacgta
     5821 cgcgatcggg tgcacgccgt ggtcgagcag ccacgcgatc gcgaccgcgg gatcgtcgta
     5881 cacgcagaat cccgacgcgc gagccgccat cgcgtggtgc agccccccgg cggcgttgaa
     5941 cgcgtgaagt accgagccgt cacggacggc ctcggccgcg acgagcgacg cgcccgccac
     6001 gagcgcgccg gcctcgtgca tgttcgggaa gatcgggttg tcgccgggcc cgaacccgta
     6061 tcggccccag tcccccgccg cgccgtgccc cgccttcctc gtcgcctcga tgtaggcgcc
     6121 cgagtgcacg aggccgatct cggcgtcgtc cgcggagcga cacgtgagcc gggaaacgtt
     6181 cggccgctcg aggagcccgc acgcctggat cagcgaccac gtatagagga cccgctgcgg
     6241 gcgcaacgga tgctgggggc cgtgatcgta gatgcgcgcc tcggggcaca gaacgaggcc
     6301 cgcgatgccg ctcacgcggt ccgcgggggt tcttcgcctt cggccgcgtc cgccggcggg
     6361 gcctggccga ggagcgactt gagtcgtttc gctcgctcct tgcgaccgcg ccggatgtcc
     6421 tcgacccaat cgggttcggg ctccccacgc agatcgggca ggtctccttc gccgcgggaa
     6481 ccttcggtgt ccatcgctag gacctgtaca tgccttgggg atcgagttgc gtgcggatca
     6541 accgcagctc gtcctcggtc ggagacgggg tctcgcccag ggacccggag atccgcggat
     6601 cccaccccat gttcgcgcgg acgtcgtcga tgctcacgcc cggatgcacc gtgagcagcg
     6661 tcatctcgcc ggtctcctcg tcgaagccga acgtcgcgag atcggtcacc acgctggtgg
     6721 gcccgctccc gaaccacccg cgcgcgcgat cgtgcgccgg atcgcccgag tgcccgggcg
     6781 aggtccggaa gtccaggtcc gcgacgaacg agcgtttcgc ctggcgcatg atgaccagga
     6841 tcctgcgcgc gtggatcgcg atctcgcacg cgccgccgga gccggggagc cggaccttcg
     6901 gcgcggcgta gtcgccgatc acggtggtgt tcaggttgcc gaaccggtcg atctgggcac
     6961 cgccgaggaa cgcggcgtcg atcaggccgg cctggaggta gaacgcgaac agctcgaaca
     7021 tcgacgtcac ggccatcgaa ccggatacga gcgtcggatc gccgatcgag agcgggagcc
     7081 gttcggggcg ggcgccgaac acgccggcct cgtacaccag ctggagctcg ggggacaccg
     7141 tgcgttgcgc gaggttgcag acgatgttcg gcaacccgac cccgacgaag cagacgcgct
     7201 cgccggcgag ggtgcgcgcg ctcgcggcga tcatcatctc gttcttggtg tagccgtcct
     7261 cgctcaccgg ggcgctccgc cgatcaggag tagtcaccgt agttcacctc gccggacagg
     7321 cgctccccgg cccgcagtcc ctcccatcga tcgcccagcc gggccacgta ctcggcgtga
     7381 ccctgcagac cgtggaccca ctccttcaac caacgttcga gcgcctccgg gtccttggag
     7441 atcccctccc actccaggta gaaggcgttg tcgcggtcgt agtagccctg ggcatagctc
     7501 gggtggcagg cgaacggctg ctcgaccacg gcgtcgacca cgacccccgg gatgacggtc
     7561 cggttcgggt cgcgacggac gaccgcctcg tccacgatct cctcgcacac cacgatcacg
     7621 cgttcggcgg cgaacgcggc ctccttctga catccgagca acccccagat ctgcgtgtcg
     7681 ccggtggccg tggcccgctg cgcgtggacg atcgtgacgt cgggccgcag cgggggcacc
     7741 acgtagacct cggtcccgtc ctggtacggg gaccgcatcg gcttgatgtt cgggttcacc
     7801 gaggggatgt cgctctcgta gtacgagcgg atcgggaaga acgggaggtt cgaggcgccc
     7861 gccacgtacc ggcacaccat gccgaagtgc gagtactcct cgatctccag gggttcgggg
     7921 tcggcctgct cgatccggcg ccgaacggcg tgcagcgagc cgacgccggg gttgccgagc
     7981 caggagaaga tcagcttccg ggcgaccccg gcgccgatca tctggtcgta gatcacgtcc
     8041 ggcgtcatac gcgcgagcgt gaggtcgcgc ttgcgctggc ggatgatctc gtggccggcg
     8101 gcgaagcaga tcaggtgggt gaagccctcg atcgcgaccg tgtcgccgtc gcggacgagc
     8161 tcgccgaccg cgtcggccat cgtggcttgc ttactcgtcc ccgcccggcc gctcgtcccc
     8221 gcccaaacgg tcactcgggg ctccagccgt cctccgggcc gcgcaggacg cagaactcgt
     8281 tgccctcggg gtcgagcatc acggtccaga agctcccgcc ctcgttgacg tagcggaact
     8341 cgctggcgcc gagctcggag acgcgggcct tctcgtccgc catcgacgtc gccggccgaa
     8401 ggtccaggtg gaggcggttc ttcacggcct tgctctcggg gacggattgg aacaggatcg
     8461 gccagccctt ccccctcggg tcgcggatca gcgcgtcctc gtcgtcgatc tcgaccacct
     8521 cgtaaccgag gaccccggcc cagaaccgcg cgaccacctc aggcgatcgg cagtcccacg
     8581 tcaaggtggc gaccgacaag gccatcgaac gcccggccgc tagccgcccg cgacgtcggc
     8641 cacggcgtcg tcgaagacct gcagggcggt ctcggcctga tcgggtcgga acaccaatgg
     8701 gggcgacatc ctgatcaccg agtccccggc gccaagtacc agcagccccc gccggaacgc
     8761 ggcctgctcg accgcgtgca tcgtctgccc gtccgcgaac tcgatgccga tcatcaggcc
     8821 ccggccgcgg acgtcgacga tctcgggccg gcgtgcctgg atgtcgcgca ggcccttcac
     8881 caagagctcg ccgacgtccc gcgcgttgtc gagcagcaca ccctcgatca gctcgagggt
     8941 cgcgagcgcg gcggcgcatg cgaccgggtt gcccccgaag gtcgagccgt gcgcgcccac
     9001 gtcccacgtc atcaggcgat cgcgagcgac gatcgcgccg agcggcatcc cgctcgcgat
     9061 ccccttgccc gccagcagga cgtcgggctc gatgctctcg tgctcgatcg cccacatctt
     9121 gcccgtccgg cccatccccg actggacctc gtcggccacg agcaggatcc cgtgccggtc
     9181 gcacagctcg cgcaggtacg cgaaccagcc ctccggcggg aagacgtacc cgccctcgcc
     9241 caggacgggc tcgaccacga tggccgccac ctcgtcgggc gggatcaggc gcgcgaacac
     9301 cacgcgctcc agatgctcga agtcgccgaa gtgcgtgtgc accacgcccg ggagcagcgg
     9361 tccgaacccc gcgtggtact tcgccttgct ggcggtgagc gagacgctcc cgtacgaacg
     9421 gccgtggaac gacccgatca tcgcgaccac gtactgcctc ccggtcgcgt agcgcgcgag
     9481 cttgatcgcg ccctcgaccg cctcggtacc cgagttcgtg aggaagctgc gggccccgcc
     9541 ggagaaaggc gcgatccggt cgagctcctc gcacacgtcc gagtagatcg gcaggtagaa
     9601 gtcgctcgcc gaatagtgca ggagctgctc ggcttggagc tggatcgcct caaccacctt
     9661 ggggtgcgca tggcccgtcg aggtcacggc gatccccgcg ttcacgtcga ggaagcggtt
     9721 cccgtcgacg tcctcgatca ccgagccggc gccgcgactg ggtacgaggt cgtaggcacg
     9781 cgggagcgag gggctcgtga ccttgcggtc gcgttcgatc cgggcgcggg cctcggggcc
     9841 cgggatctcg gtgaccagcc gcggcgcctg cgcctcggcc aggtccacgg tctccgccgt
     9901 gctcactcct cgatcaccgt gcgcgactgc tcccgcaggt actgctgcac gtagtagggg
     9961 ccgcctccgg ccttgccgct ggtacccgag cccttccagc cgccgaaggg ctgcacgccc
    10021 ggccacgcgc ccgtggtcgc tcccgccctc ctgttcacgt acacgacgcc ggcctcgatc
    10081 gagctcaacc accggtcgat ctcggctcgg tcctcgctgt agaagccggc cgtcaggccg
    10141 tactccgtgt cgttggacag acggatcgcc tcgtcgaggt cgtcgtacgg cgcgaccgcg
    10201 acgaacggca cgaacagctc cttcttccag atccacgagt cgagcgggac ctcggcgatg
    10261 gtgggctgga cgaacgttcc cttcgcgagc tcgccctcgg tgagccgctc gccgcccgtg
    10321 accaccgtgc cgttcttttt cgcctcgttc gacgcctcct cgaaggtcgt gaccgcgctc
    10381 tcgtcgatca ccgggccgag gtagacgtct cggtcgagcg ggtcgccgat cttcacgtcc
    10441 tcggtcttct tcctgagctg gcggacgaac tcgtcgtaga cgctccggtg cacgtagaca
    10501 cgcgagttgg ccgagcactt ctgcccgccg aacccgaacg ccgaccgcag caccccgtcg
    10561 gtcgccttgt ccagatcggc cttgtcggtc acgatcgtcg ggttcttccc gcccatctcg
    10621 cagatcacgg gcttggggaa gtccttcgcg aaccccttgt agatccccat ccctacctcg
    10681 tacgagccgg tgaaggtgat ccccgccaca tccgggtggt tgacgatctc gtcgccgacc
    10741 acgctgcccg gacccgagag gaagtggaag acgcccttcg ggacgccggc cgccatgtag
    10801 acctcgtaca gcttcagccc ggtgaacacg cccatgtggg cgggcttgaa caccacggtg
    10861 ttgcccgcga ccagcgccgc ggacgacggg cccgccgcga gcgccatcgg gaagttgaac
    10921 gggctgatca cggcccacac gccgtacggc cgaaggacgt cgtagtactc gcccggcgag
    10981 ccgagcgact gcatcggctt gcggaagccc tcgttctgct cgacctcgtt ggcgttccac
    11041 cgcagcaggt ccgcggactc ctccacgtcg ccgagcgcct cgagccggtt cttgccgacc
    11101 tccatcgcca tcagcgccga cagctcgttg cgctgatcgg acatgatgtc ggcggccttg
    11161 cggatgatcg ccacccgctc ctgccatggg cggtcggccc agtcgtggaa cgcgcccttg
    11221 gcggccgcga cggcgtcgcg gacgtcctcg cgcgtgcccg aggcaaactc gccgatcagc
    11281 gtgccgtcga tcggtgaatg ggcggccttg tacccggagc cgccgcgtgc ctcgccgttt
    11341 acgaagagcg gatgccgctg cccgagccat gaccgggcca cctcgacgcc ccggtcgtag
    11401 gcctcgtgca tgtcctcgtt gtcggccgcc atcgtcgcgt aggtgatgcg gaacccttcc
    11461 tctgccatgg agtgcctcct atgcgcggaa acggcgcgtg acctggtcga tctgggtctc
    11521 aatcgtagtc gccggagccg gtttccccca agtcggcggc cgcggcgaac tgcccgatcg
    11581 ataccgagat tcggcgcccg gccgggcgta tcatcggcct cccttgaggc tcgtgacctt
    11641 cgaccggcgg gggcaccggc gactgggagc gctccgggaa gggaacgtcg tggatctgcc
    11701 gagcctgctc ggccaccccg cgttcccgga gacgatggag cggctcgtca ggagcaacgg
    11761 gggaacggtg ctcgatgccg cccgggcagc cctcgagcgc gacgaggcag atgacttcgt
    11821 cgtcgaacga ccacgtctgc tcgcggcgct cctgccgac
//
LOCUS       DATBRK010000426         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1111679, whole genome shotgun sequence.
ACCESSION   DATBRK010000426 DATBRK010000000
VERSION     DATBRK010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111679"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="VIH70_12635"
     CDS             complement(<1..123)
                     /locus_tag="VIH70_12635"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY8117255.1"
                     /translation="MAVIKTIDLVGVSSESWRDAAHQALSEASKTIRGIESMDIL"
     gene            complement(132..1037)
                     /locus_tag="VIH70_12640"
     CDS             complement(132..1037)
                     /locus_tag="VIH70_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEY8117256.1"
                     /translation="MALGYDKPLHILAFDHRGSFQKKFFGVTGEPNEEETARISDAKR
                     VIYEGARRALDEGVEAEAAGVLVDEQFGADIARDAKTAGFRLAMPVEKSGQEEFDFQY
                     DEDFGSHIEAFDPNFSKVLVRYNTEGDRAMNERQAGRLGRLGDWLHEHGRLFLFELLV
                     PPTPEQIAASGGDERRWDSEDRPMLMKAAIEELQDAGVEPDIWKIEGIDRREDCEVIS
                     ATTRREGRDGVACVVLGRGADDAAVDHWLRMGSGVPGYIGFAIGRSIWWDPLKGFVDG
                     NLEREEAAKQIAANYRRFIGVYQGR"
     gene            complement(1078..1725)
                     /locus_tag="VIH70_12645"
     CDS             complement(1078..1725)
                     /locus_tag="VIH70_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8117257.1"
                     /translation="MGLDGVVTWFRQASFRWQGDGPVVYVDPWGVQDDVPADVILITH
                     AHFDHLQPSEVERLRTSSTTIVAPRDVADELTGDVTAIEPGGSVDAGGLKIQAVPAYN
                     TAEERLDMHPQKNGWVGYVFEFGGRTFYHAGDTDALPELESLDPDVAIVPIGGTYTMD
                     WQEAAGFVKAMEPRTAVPMHYGFVVCSPSHAGRFRKGAQPVPVEILEPTNPFEQP"
     gene            1824..>2284
                     /locus_tag="VIH70_12650"
     CDS             1824..>2284
                     /locus_tag="VIH70_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8117258.1"
                     /translation="MTLLRRNRNFRRLYLASVISLGGDWFLLIALFGLMLDLTGQAIA
                     VAFTIAAQDLTYFLASPFAGVLADRIDRRRLMIGCDLARAVLVLGFLLVRTDDLTWLV
                     YVLLAATAVFAAAFEPASMAAMPNLVDERDLSIANALSGSLWGTMLAVGGAL"
BASE COUNT          314 a          852 c          708 g          410 t
ORIGIN      
        1 gaggatgtcc atcgactcga tcccgcggat cgtcttcgac gcttccgaga gcgcctgatg
       61 ggcggcgtcg cgccacgact cggacgagac cccgaccaga tcgatcgtct tgatgaccgc
      121 catcgccact cctacctccc ctgatagacg ccgatgaaac gccggtagtt ggcggcgatc
      181 tgcttcgcgg cctcctcgcg ctcgaggttg ccgtcgacga accccttcag cggatcccac
      241 cagatcgagc ggccgatcgc gaacccgatg taccccggca cgcccgaacc catccgcagc
      301 cagtgatcga ccgcggcgtc gtcggccccg cggccgagca ccacgcacgc cacgccgtcg
      361 cgcccctcgc gccgtgtcgt tgccgagatc acctcgcagt cctctcgccg atcgatgccc
      421 tcgatcttcc agatgtcggg ctccacgccg gcgtcttgga gctcctcgat cgccgccttc
      481 atcagcatgg gccgatcctc cgagtcccag cgccgctcgt cgccgcccga ggccgcgatc
      541 tgctcggggg tcggcgggac gagaagctcg aacaggaaca gccgcccgtg ttcgtgcaac
      601 cagtcgccga gcctgcccag ccggccggcc tggcgctcgt tcatcgcccg gtcgccctcg
      661 gtgttgtagc ggaccagcac cttggagaag ttcgggtcga acgcctcgat gtgcgagccg
      721 aagtcctcgt cgtactggaa gtcgaactcc tcctgcccgc tcttctcgac cggcatcgcc
      781 agccggaacc ccgcggtctt cgcgtcgcgg gcgatatcgg ctccgaactg ctcatcgacc
      841 agcaccccgg cggcctccgc ctcgacccct tcgtccagcg cccgtcgggc accctcgtag
      901 atcacgcgct tggcgtcgga gatccgcgca gtctcctcct cgttgggttc gccggtgacg
      961 ccgaagaact tcttctggaa cgagcctcga tggtcgaacg ccaggatgtg cagcggcttg
     1021 tcgtatccga gcgccatctc gtcactctcc tctgtgtctc ggcacgtctt ctcgacccta
     1081 cggttgctcg aacggattcg tgggctccag gatctcgacc ggcacgggtt gcgcgccctt
     1141 cctgaacctc cccgcgtgcg acggcgagca cacgacgaag ccgtagtgca tcgggacggc
     1201 cgtccgcggc tccatcgcct tgacgaaccc ggccgcctcc tgccagtcca tcgtgtaggt
     1261 cccgccgatc ggcacgatcg cgacgtccgg gtcgaggctc tcgagctccg gcagcgcgtc
     1321 ggtgtccccg gcgtgataga aggtccgccc gccgaactcg aacacgtacc ccacccagcc
     1381 gttcttctgc ggatgcatgt ccagacgttc ctcggcggtg ttgtacgcag ggaccgcctg
     1441 gatcttgagc ccgccggcgt cgacggatcc acccggctcg atcgccgtga cgtcgccggt
     1501 cagctcgtcg gccacgtctc gcggggccac gatcgtggtc gacgacgtgc gcaggcgttc
     1561 gacctcactc ggctggagat ggtcgaagtg cgcgtgcgtg atgaggatga cgtccgccgg
     1621 cacgtcgtcc tgcacgcccc agggatcgac gtacacgacg ggaccgtcgc cctgccatcg
     1681 gaacgacgcc tgccggaacc acgtgacgac cccgtcgagc cccatccgtc ggcctccttc
     1741 ggtcgtgtcc tgcgccagta gtcttgcaaa tcgacgtgga agcgcgcgaa ccggtcgaga
     1801 ccctaggcgg ctttcgggcc tccgtgaccc tgctccggcg caaccggaac ttccgccgcc
     1861 tgtacctcgc ctcggtgatc tcgctgggtg gagactggtt cctcctgatc gccctgttcg
     1921 ggctgatgct cgacctcacc gggcaggcga tcgccgtggc gttcacgatc gccgcccagg
     1981 acctgaccta cttcctcgcc tcgccgttcg ccggggtgct ggcggaccgg atcgaccggc
     2041 ggcggctcat gatcggttgc gacctcgccc gggcggtcct cgtcctcggc ttcctcctgg
     2101 tacggaccga cgatctcacc tggctggtct acgtcctcct ggccgcgacc gcggtgttcg
     2161 cggccgcgtt cgagccggcc tcgatggcgg cgatgccgaa cctggtcgac gagcgcgacc
     2221 tctcgatcgc gaacgccctg tcggggtcgc tgtggggcac gatgcttgcg gtcggcggtg
     2281 ccct
//
LOCUS       DATBRK010000427        10438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2698463, whole genome shotgun sequence.
ACCESSION   DATBRK010000427 DATBRK010000000
VERSION     DATBRK010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10438
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2698463"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VIH70_12655"
     CDS             <1..1060
                     /locus_tag="VIH70_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEY8117259.1"
                     /translation="GISGQVYLFKNNTDSAGNSYGCHENYLVARQGEFARMADLLIPF
                     FVTRQIWCGAGKVLHGPRGAVFCIAQRAEHIWEGVSSATTRSRPIINTRDEPHADAER
                     FRRLHVIVGDSNMSEFSSWMKVGVTDLVLRMLEANTVMRDLSLENPIRAIREISHDVT
                     CRKKVRLANGRELSAIEVQEEYYERTERFLERRGPDPHSKQLLQEWREALDALILQEP
                     ERLVRKVDWVAKQSLIENYRQRHQLLLSHPRVALLDLAYHDVNRTRGLYYVLERAGKM
                     ERAVAEKEIQRAMREPPQTTRAKLRGDFIRHAKQKRRDYTVDWVHLKLNDQAQRTVLC
                     KDPFRSVDERVEQLIAHM"
     gene            complement(1118..2260)
                     /locus_tag="VIH70_12660"
     CDS             complement(1118..2260)
                     /locus_tag="VIH70_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEY8117260.1"
                     /translation="MSPGPPTGSVRLRHATVRRIRSERPGVLELDVVVDDDPADPPPV
                     AEPGVAVAFTQLVGSVEVGDRVLVNTTGVALGLGTGGFHLVVAVDDQTATELGHPGRV
                     MKARYTPLQAAVESVEETDRETLERSEGLGGRPVVCAPLHSMIGPIAAGARAAGAERI
                     AYVMSDGAALPGRLSNLVPRLRVAGLLDGFVTSGQAFGGELEAVTIWTGVLAAAELVG
                     ADVVIVADGPGNLGTDTTWGVSALASGHALNATEALGGRPVAALRVSFADGRERHRGV
                     SHHSLTILDRVCRVAADVAVPVLEGRERDLVWDSLRERRLEERHQLVEVDGRPALDEL
                     ERAGVEIDSMGRTASEDPAFFLAAGAAGILAGRMAAGSRRWRRGAG"
     gene            2284..3225
                     /locus_tag="VIH70_12665"
     CDS             2284..3225
                     /locus_tag="VIH70_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY8117261.1"
                     /translation="MHPLERLVNLVALLLESRTPLTFDQIRSKLAEGYGHQDASSAKR
                     MFERDKDVLRDIGVPIDVVSTDAWDVEHGYTIDKDRYYLPDIAFTPEELSALFVAVRS
                     AGDVSAEEAVRKLLSGADRGILAGLGPPALAGETDPNLSRAAEAVAEGRRVRFAYRTS
                     RGERSDRTVDAFGLAVRGGHWYLVGLDLDRAEIRSFRLSRISDELEIAGEGSEPPVGF
                     RAVDHVQAGPWGPGDAETTATVAFAPEVAWWATKGIAGADTVRIREDGWVEVRIAWSP
                     GEGLMSWILSFGPDAEVAEPPELRSEILGRLEEILAS"
     gene            3215..4207
                     /locus_tag="VIH70_12670"
     CDS             3215..4207
                     /locus_tag="VIH70_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY8117262.1"
                     /translation="MPPEAGRRSRATRKAPEKASERLRRLLTVVPYLVRNQGTRVAEA
                     ADLFGMSEPELLADLDLLFVSGLPPYGPGDLIDVDIQDGRIWIGMADYFARPLRLTRT
                     EALAIYLRGTVLAGAPGLAEAPALSSALGKLADALGPETLGDLPERVDSAGDGRTPDT
                     LGELRRAASERERLRIEYYAASGAETTDREIDPEEVFFAIGNWYVAAWDHRSNSERLF
                     RADRIHAAAATGKRFEPRGLRGAGRPLYTPSEEDVRVIVRLRPEARWVAEYYETSREV
                     ELGDGRLEVELPAGRFEWLERLLLRLWPDAEVVVPPGLHERVRELAERTRERYR"
     gene            4397..4639
                     /locus_tag="VIH70_12675"
     CDS             4397..4639
                     /locus_tag="VIH70_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117263.1"
                     /translation="MEPVEKRADRKIVALLVSAGVDVSGRSPAGTAEMFEPDVDLPQD
                     PRGPLPPGPAFSIDDVIDFHFKLENERYIEQFLTAQ"
     gene            4679..4900
                     /locus_tag="VIH70_12680"
     CDS             4679..4900
                     /locus_tag="VIH70_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117264.1"
                     /translation="MTTTALVWLVVGLVSLVAVLAVLIALIRHLLVLGRAARRFQDEV
                     GPLTQEIGELADAASGRGRGVPRGPRSGR"
     gene            4974..5549
                     /locus_tag="VIH70_12685"
     CDS             4974..5549
                     /locus_tag="VIH70_12685"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY8117265.1"
                     /translation="MLNIGPQELLIILVIALIVVGPQRLPALGRSIGRGLRELRKAQD
                     EVRRTIQVNLDDEPPNGSEARPTGHPRPTGSADGRTADPGVAMGAGSDSTPSASEPLP
                     PDEIREISRSLGRSLAELRRAKEEVRRSFRIDLVHPAKPPEQERPRPSEPSTAPADAG
                     GSSATPASSPAASTPEPSPDGRSVTPDRPID"
     gene            5560..6345
                     /gene="tatC"
                     /locus_tag="VIH70_12690"
     CDS             5560..6345
                     /gene="tatC"
                     /locus_tag="VIH70_12690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY8117266.1"
                     /translation="MMKLIPERLWRRSEPARDGTMTLVDHLSELRYRLLVMIAAIAVG
                     AIFGWFLFDRVIDLLLNPYCDYWRTVPLRLRATPDCALFFSGALEPLLIKFKLVAFLG
                     LFLALPVVLYQLWAFIVPGLTRRERKMAIPFVVSSVLLFALGALVAYWTLPKALNFLL
                     GFAGQGFAPLLTGDRYLTFVMLVALSFGLAFEFPIVLIFLLLAGAVTTRQLRDWRRGA
                     ILGIAVFAAIITPSSDPYTMLAMTIPMVLFYEGAIIVGRLLKR"
     gene            6357..7100
                     /locus_tag="VIH70_12695"
     CDS             6357..7100
                     /locus_tag="VIH70_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117267.1"
                     /translation="MAIKGKTKGRGAKAVTRGPKPVYQPVKKRLFAKREFWLVILSIL
                     GVMVVVGLVAGFIAERTSNAQDELEQRMRATMSSFQGQVDSVLTTIGQARPPAGYKVF
                     TDLSTAVDSLVAETPDAPVDPKALTQPATEAATSARTALEALQSIDETALIRGKGFSE
                     DFVLYVINAKTNFVGAMTLYGEAAKLLELAADAQGTERAELAARTKAILKSATDMFDR
                     GYGDYVEAQTKAGVFAPSAPGLPAPTGAS"
     gene            7097..7831
                     /locus_tag="VIH70_12700"
     CDS             7097..7831
                     /locus_tag="VIH70_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8117268.1"
                     /translation="MTDGLLLLHAWPVDARMWEPQLAALPDGLAVAAPHLPGFGGSEP
                     AGDVLTMGEAAERALDALGERGIDRAVVCGLSMGGYVAFELWRRARDRVIGFVLANTR
                     AVADPAEAAAARRALAERLRSEGNVLATEPPALLSDRASQDLRARVRGWIAEQPPDSI
                     AAASLGMAERPDSTPDLATIDVPTLVLTSTADRLIAPEISAEMAGLIPQGRLETIEGV
                     GHLSNLEAPEAFDAALLEHLAACGLR"
     gene            7884..>10438
                     /locus_tag="VIH70_12705"
     CDS             7884..>10438
                     /locus_tag="VIH70_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8117269.1"
                     /translation="MIDPARFAARYPFELDAFQVRAIEALHDGESVLVAAPTGSGKTV
                     VAEFAVDRALAADRKCFYTTPLKALSNQKFGDLVARLGAAKVGLLTGDNAINGDAPIV
                     VMTTEVLRNMLYERSEALDGLLTVVMDEVHYLQDPYRGGVWEEVLIHLPLSISVVCLS
                     ATISNAEEFGEWIGTLRGVTRVVIEEKRPVPLEHHYLVGRELHPMHVEQNGQAAPNPY
                     VVSLDRQEVRYKTYYRRGSGTAQHQRIPRPREGHRRVYVPRREEVVEVLADQSLLPAI
                     YFVFSRAGCDRSVEWLMGAGIRLTMSEEASRIRTFAEMRVAWMDEEDLKTLGFYDFQD
                     ALAAGIAAHHAGMLPVFKETVEELFEAGLVKIVFATETLSLGINMPARSVVIEDLWKF
                     QGERHELLTPGEYTQLTGRAGRRGIDELGHAVVVVQRNVAFERVASLASTRTYELTSS
                     FRPSYNMAVNLVRNYTRDEAHHLLNSSFAQFLADRGVVTLERELERDRAQVAGFRERM
                     RCHLGDFAEYWALREKAMAVRDEFRKGRERARTESIREGLAALRPGDVIFVPRARRRG
                     LAVVLSAREGRPTVLAQDRTFFRLGLRDFQEPPTVLTKVPLPRSGSVRSARYRRDLAA
                     RLVALDVRPPRKPQGRVDARVEREAARYERLAEAHPCHACPDRPEHERWATRASQVEH
                     QIRGLDRRIRTRTETLARQFERVIAVLEELGYLRSFAIEPKGRTLARIYGEGDILMAE
                     ALADGLLEGLDPSEVAAVVSTVVYESRERTPRAAEMPTPASVERYAAFRRIWRRVREA
                     EDRHQVELCRELEDGFAAPIHRWAQGESLEDVLRETDMSPGDFVRTCKQVLDLL"
BASE COUNT         1528 a         3660 c         3724 g         1526 t
ORIGIN      
        1 gggcatcagc gggcaggtct acctgttcaa gaacaacacc gactcggcgg gcaactcgta
       61 cgggtgccac gagaactacc tggtggcccg gcagggcgag ttcgcgcgca tggccgacct
      121 gctgatcccg ttcttcgtga cgcggcagat ctggtgcggc gcgggcaagg tcctgcacgg
      181 cccccgcggc gccgtcttct gcatcgccca gcgcgccgag cacatctggg agggcgtctc
      241 gtcggcgacc actcgctcga gaccgatcat caacacgcgc gacgagcctc acgcggacgc
      301 cgagcggttc cgccggctgc acgtcatcgt cggggactcg aacatgtcgg agttcagctc
      361 ctggatgaag gtgggcgtca ccgacctggt ccttcggatg ctcgaggcga acacggtgat
      421 gcgcgacctc tcgctcgaga acccgatccg ggcgatccgc gagatcagcc acgacgtgac
      481 ctgtcgcaag aaggtccgcc tggccaacgg gcgggagctt tcggcgatcg aggtgcagga
      541 ggagtactac gagcgcaccg aacgtttcct ggagcgacgc ggtcccgatc cgcactccaa
      601 gcagctgctg caggagtggc gcgaggcgct ggacgccctg atcctgcagg agccggagcg
      661 gctggtgcgc aaggtcgatt gggtcgccaa gcagagcctg atcgagaact atcgccaacg
      721 ccaccagctg ctgctctccc acccgcgcgt cgcgctcctg gacctcgcgt accacgacgt
      781 caaccgcaca cgcggcctgt actacgtgct cgagcgcgcg ggcaagatgg aacgcgccgt
      841 ggcggagaag gagatccagc gggccatgcg ggaacccccg cagacgacgc gggccaagtt
      901 gcgaggggac ttcatccgtc acgcgaagca gaagcgtcgc gactacacgg tggactgggt
      961 ccacctcaag ctgaacgacc aggcccagcg aacggtgctc tgcaaggacc cgttccgcag
     1021 cgtggacgag cgcgtcgagc agctgatcgc ccacatgtga cgaggtccat cggccgagcg
     1081 ccgacggacc gacacgcgac ggcgggtgag gtcggcttca gccggcgccc cgtcgccaac
     1141 ggcgcgatcc cgccgccatc ctgcccgcaa ggatgcccgc ggcacccgcc gcgaggaaga
     1201 acgccgggtc ctccgacgcc gtccggccca tcgagtcgat ctcgaccccc gcccgctcga
     1261 gctcgtcgag cgcaggccgc ccgtccacct cgaccagctg gtggcgctcc tcgaggcgtc
     1321 gctcgcgcag cgagtcccac acgaggtcgc gctcgcgtcc ctcgaggacc gggacggcga
     1381 cgtcggccgc cacccggcac acccggtcca ggatcgtgag ggagtggtgc gacaccccgc
     1441 ggtggcgttc gcggccatcg gcgaacgaga cgcgcagcgc cgccacgggg cgcccgccga
     1501 gcgcctccgt cgcgttgagg gcgtggccgc tcgcgagcgc gctcacgccc cacgtcgtgt
     1561 cggtgcccag gttgcccggc ccgtcggcca cgatcacgac gtccgcgccg acgagctccg
     1621 cggcggcgag cacgccggtc cagatcgtca cggcctcgag ctcaccgccg aaggcctggc
     1681 cgctcgtcac gaaaccgtcc aggagccccg cgacccgcag gcgcggaacg aggttcgaga
     1741 gccgacccgg aagcgcggcc ccatcggaca tcacgtaggc gatccgttcc gcgccggccg
     1801 cccgcgcccc ggcggcgatc ggtccgatca tcgaatgcaa cggcgcgcac acgaccggcc
     1861 tgccgcccag gccctcggag cgttcgagcg tctcgcgatc ggtctcctcg acgctctcca
     1921 ccgccgcctg caacggcgtg taccgagcct tcatcacccg tcccggatgg ccgagctcgg
     1981 tggcggtctg gtcgtcgacc gccacgacca ggtggaagcc gcccgtcccg agaccgagcg
     2041 cgacccccgt ggtgttcacc aggacgcgat cgccgacctc cacgctgccg acgagctgcg
     2101 tgaacgccac cgcgaccccg ggctcggcga cgggcggcgg gtcggccgga tcgtcgtcca
     2161 cgaccacgtc gagctcgagc acgcccggac gctcgctccg gatgcgccgg acggtggcgt
     2221 gacgcaggcg cacgctccct gtcggggggc ctggcgacat ggcggaagta tgatgccgcg
     2281 gccatgcacc cgctcgaacg cctggtgaac ctcgtcgccc tgctgctcga gtcccggacg
     2341 ccgctcacgt tcgaccagat ccggagcaag ctggcggagg gatacgggca tcaggacgcg
     2401 tcgtcggcca agcgcatgtt cgagcgcgac aaggacgtcc tgcgcgacat cggggtgccg
     2461 atcgacgtgg tctcgaccga cgcgtgggac gtcgagcacg gatacacgat cgacaaggac
     2521 cgctactacc tccccgacat cgccttcacg ccggaggagc tctcggcgct gttcgtcgcg
     2581 gtgcgttcgg cgggcgacgt gtcggcggag gaggccgtcc gcaagctgct ctcgggcgcc
     2641 gaccgcggga tcctggccgg cctcgggccc ccggcgctcg ccggcgagac cgacccgaac
     2701 ctgtcccggg ccgccgaggc ggtcgcggag ggacgtcgcg tgcgattcgc gtaccgaacg
     2761 tcccgaggcg agcgctcgga ccgaaccgtg gatgcgttcg ggctggcggt ccgcggggga
     2821 cactggtacc tcgtcggcct ggatctggac cgggcggaga tccgttcgtt ccgcctctca
     2881 cggatctcgg acgagctcga gatcgcgggg gaggggagcg agccccccgt cggtttccgc
     2941 gcggtcgacc acgtccaggc cgggccgtgg ggtccgggcg atgccgaaac gaccgcgacc
     3001 gtcgcgttcg cgccggaggt cgcgtggtgg gcgacgaagg ggattgcggg ggcggacacg
     3061 gtccgaatcc gggaggacgg ctgggtcgag gtgcgcatcg cctggtcgcc gggggagggg
     3121 ctgatgtcat ggatcctgtc gttcggaccc gacgccgagg tcgcggagcc ccccgaactg
     3181 cgatccgaga tcctcggacg gctggaggag atccttgcct cctgaggcag gtcggcgttc
     3241 gcgcgcgacc cgcaaggccc ccgagaaggc gtccgaacgg ctccggcggc tgctcacggt
     3301 ggtcccgtac ctggttcgca accagggaac gagggtcgcg gaggccgcgg acctgttcgg
     3361 gatgtccgaa cccgagctgc tggccgacct ggacctgttg ttcgtctcgg ggctgccgcc
     3421 ctacgggccg ggagacctga tcgacgtcga catccaggac ggccgcatct ggatcgggat
     3481 ggccgactac ttcgcacggc ccctacggct cacgcggacc gaggcgctcg cgatctacct
     3541 gcgcgggacc gtgctggcgg gcgccccggg tctggcggag gcgcccgccc tgtcctcggc
     3601 actcgggaag ctggcggacg cgctgggccc cgagacgctc ggggacctgc ccgagcgggt
     3661 ggactcggcg ggagacggac gaactccgga cacgctcggc gagctgcgcc gtgcggcctc
     3721 ggagcgtgag cgcctgcgga tcgagtacta cgcggcctcc ggggccgaga ccaccgatcg
     3781 cgagatcgac cccgaggagg tcttcttcgc gatcgggaac tggtacgtgg ccgcgtggga
     3841 ccaccgctcg aactcagagc gcctgttccg tgccgaccgg atccacgccg cggcggccac
     3901 cgggaagcgg ttcgagccgc ggggcctccg gggggcgggc cggcccctgt acacgccttc
     3961 ggaggaggac gttcgcgtga tcgtccgact tcggcccgag gcgcgatggg tggccgagta
     4021 ctacgagacc tcgcgcgagg tggagctcgg ggacggccgc ctggaggtcg agctccccgc
     4081 cggcaggttc gaatggctcg agcgcctctt gctccgcctg tggccggacg ccgaggtggt
     4141 cgttcccccc gggctgcacg aacgcgtccg cgagctggcc gaacggaccc gcgaacggta
     4201 ccgatagagc gtcgtcgctt cctcggcccg ggggctcaag cgcggcgcac cgtcgtccga
     4261 tattgcgggt gttatgacga cgatccggac gacatgcccc cgctgcggag aggtcgacat
     4321 ggggcccgag tcgattctgc ttcaggtcat gcgaggcggg cgagagggca cctatcgttt
     4381 cgactgcccc acctgcatgg agcccgtcga gaagcgcgcg gaccgcaaga tcgtcgcgct
     4441 gctcgtctcg gccggcgtcg acgtctccgg gcgatccccc gctgggaccg ccgagatgtt
     4501 cgagccggac gtcgatctcc cccaggaccc gcgagggccg ttgcccccgg gacccgcgtt
     4561 ctcgatcgac gacgtgatcg acttccactt caagctcgag aacgagcgtt acatcgagca
     4621 gttcctcacc gcgcagtaaa ccccgctcgg cgggaccact ccgctagcat cgatgccgat
     4681 gaccacgacc gcgctcgtct ggctcgtcgt cgggctcgtg tcgctcgtcg ccgtcctcgc
     4741 agtgttgatc gcgctgatcc ggcacttgtt ggtgctcggc cgcgcggctc gccggttcca
     4801 agacgaggtc gggcccctca cgcaagagat cggcgagctc gcggacgccg cctccggccg
     4861 gggccgaggg gtgcctcggg gacccaggtc cggccgctga accgaggcgg cagtacgatg
     4921 gcgctcgcga gggcttctcg cccccggatc gcggacctgg agcacaggac gagatgctga
     4981 acatcggtcc acaggagctc ttgatcatcc tcgtgatcgc cctgatcgtg gtcgggccgc
     5041 agcgcctgcc cgcgctcgga aggtcgatcg ggcggggtct gcgcgaactc cgcaaggcgc
     5101 aggacgaggt caggcggacg atccaggtca acctcgacga cgagcctccg aacggctctg
     5161 aggcgcgtcc gaccggtcac ccgaggccca cgggttccgc cgacgggcgc accgccgacc
     5221 cgggcgtggc gatgggcgcg gggtccgact ccacaccctc tgcctccgag ccgcttcccc
     5281 ccgacgagat ccgggagatc tcgcgttcgt tggggcggag ccttgcggag ctccggcgcg
     5341 cgaaggagga ggtccggcgc tcgttccgga tcgatctcgt ccacccggcc aaaccccccg
     5401 aacaggaacg accccgacca tccgagccct cgacggcgcc ggccgatgcg ggtggatcgt
     5461 cggcgacgcc ggcctcgagc cccgccgcat cgacgccgga accgtccccg gacggacgat
     5521 cggtcacgcc cgaccgcccg atcgactgac cccaggtcga tgatgaagct gatcccggaa
     5581 cgcctctggc ggcgctccga gcctgcccgc gatggcacga tgacgctcgt cgaccacctg
     5641 tcggagttgc ggtaccggct cctcgtcatg atcgcggcga tcgcggtggg ggcgatcttc
     5701 ggatggttcc tgttcgatcg cgtgatcgat ctgctgctta acccgtactg cgactactgg
     5761 cggacggtgc ccctccgcct ccgggcgacg cccgactgcg ccctgttctt ctccggcgcg
     5821 ctcgagccgt tgctgatcaa gttcaagctc gtcgcgttcc tggggctgtt cctggcgctg
     5881 ccggtggtcc tgtatcagct gtgggcgttc atcgtgcccg gcctgacacg gcgcgaacgg
     5941 aagatggcga tcccgttcgt ggtgtcctcg gtgctgctgt tcgcgctcgg ggcgctggtc
     6001 gcgtactgga cgctcccgaa ggccctgaac ttcctcctcg gcttcgccgg ccaggggttc
     6061 gcgcccctgc tcaccggaga tcggtacctg acgttcgtga tgctcgtcgc gctgtcgttc
     6121 ggcctcgcgt tcgagttccc gatcgtgctg atcttcctgc tgctcgccgg cgccgtcacg
     6181 acccggcagc tgcgggactg gcggcggggg gcgatcctgg gcatcgcggt cttcgccgcg
     6241 atcatcacgc cgagctcgga cccctacacg atgctcgcga tgacgatccc gatggtgctg
     6301 ttctacgaag gcgctatcat cgtgggccgt ctcctgaagc gctaggtgga tccagcatgg
     6361 cgatcaaggg caagaccaaa gggcggggcg cgaaggccgt tacccgcggg cccaagcccg
     6421 tctatcagcc cgtcaagaaa cgcctgttcg ccaagcgcga gttctggctg gtgatcctca
     6481 gcatcctggg tgtcatggtg gtcgtcggac tcgtcgccgg gttcatcgcg gagcgcacct
     6541 cgaatgcaca ggacgagctc gagcagcgga tgcgggcaac gatgagctcc ttccagggcc
     6601 aggtcgactc cgtgctcacc acgatcggcc aggcgcggcc accggccggg tacaaggtgt
     6661 tcacggacct ctcgacggcc gtggacagcc tcgtggcgga gacgccggac gcgccggtcg
     6721 acccgaaggc gctcacgcag ccggcgaccg aggccgcgac ctccgccagg accgcgctgg
     6781 aggccctgca gtcgatcgac gagacggcgc tgatccgggg caagggcttc tccgaggact
     6841 tcgtcctgta cgtgatcaac gcgaagacca acttcgtcgg ggcgatgacc ctgtacggcg
     6901 aggccgcgaa gctgctcgag ctcgcggccg acgctcaggg caccgaacgc gccgagctcg
     6961 cggctcgcac caaggcgatc ctgaagtccg cgaccgacat gttcgaccgc ggctatgggg
     7021 attacgtcga ggcgcagacc aaggcgggcg tgttcgcccc gtcggcgccg ggactccccg
     7081 caccgaccgg cgcttcgtga ccgacggtct cctcctgctc cacgcctggc ccgtcgacgc
     7141 caggatgtgg gagccgcagc tcgccgcact cccggacggc ctggccgtgg cggcgcccca
     7201 ccttccgggg ttcgggggct cggagccggc cggtgacgtc ctgacgatgg gcgaggcggc
     7261 cgaacgagcg ctcgatgccc tgggggagcg aggcatcgac agggcggtcg tgtgcggcct
     7321 ctcgatgggc gggtacgtgg cgttcgagct gtggcggcgg gcccgcgacc gcgtgatcgg
     7381 gttcgtgctg gccaacaccc gcgcggtggc cgatcctgcg gaggcggcgg ccgcccggcg
     7441 cgcgctggcc gaacgcctgc ggtcggaggg caacgtcctc gccacggaac caccggcgct
     7501 gctgtccgat cgtgcatcgc aggacctgcg ggcgcgggtc cgcggctgga tcgcggagca
     7561 gccgccggac tcgatcgccg cggcgtcgct cgggatggcc gagcgcccgg actcgacccc
     7621 cgacctcgcg acgatcgacg tgccgaccct cgtgctgacc tcgaccgcgg atcgcttgat
     7681 cgcccccgag atctcggccg agatggcggg cctgatcccg caggggcgcc tcgagacgat
     7741 cgagggtgtc gggcacctgt cgaacctcga ggcgcccgag gccttcgacg ccgcgttgct
     7801 cgagcacctc gccgcctgcg ggctccgctg acctcgaacg gtccgcgccg ggctccgggc
     7861 ggggcgcccg tagactcggt cccatgatcg atcccgcgcg cttcgccgcc cgctacccgt
     7921 tcgagctcga cgcgttccag gtacgcgcga tcgaggcgct ccacgacggc gagtccgtcc
     7981 tcgtggcggc gccgaccggc tcgggcaaga cggtggtggc cgagttcgcg gtggaccgcg
     8041 cgctcgcagc cgaccggaag tgtttctaca cgacgccgct caaggcgctc tcgaaccaga
     8101 agttcgggga cctggtcgcg cggttggggg cggcgaaggt ggggctgctc acgggagaca
     8161 acgcgatcaa cggagacgcg ccgatcgtcg tcatgacaac ggaggtgctg cgcaacatgc
     8221 tgtacgagcg tagcgaggcg ctcgacggcc tcctgacggt cgtgatggac gaggtgcact
     8281 acctccagga cccgtaccgg ggtggggtgt gggaggaggt gctgatccac ctcccgctgt
     8341 cgatctcggt cgtgtgcctg tcggccacga tctccaacgc ggaggagttc ggcgaatgga
     8401 tcggcacgct ccgcggcgtg acgcgcgtgg tgatcgagga gaagcgccct gtgccactcg
     8461 agcaccacta cctggtcggt cgtgagctgc atccgatgca cgtcgagcag aacgggcagg
     8521 ctgcacccaa cccgtacgtc gtctcgctcg accgccagga ggtccggtac aagacgtatt
     8581 accgacgggg ttcgggcacg gcgcagcacc agcggatccc gaggccgcgc gagggccatc
     8641 gtcgcgtcta cgtcccccga cgtgaggagg ttgtcgaggt cctcgccgac cagagcctgc
     8701 ttccggcgat ctacttcgtg ttcagccgag ccggctgcga ccggagcgtc gagtggctga
     8761 tgggcgccgg gatccggctc acgatgagcg aggaggcgtc ccggatccgg acgttcgccg
     8821 agatgcgcgt cgcctggatg gacgaggagg acctcaagac cctcggcttc tacgacttcc
     8881 aggacgccct ggcggccggg atcgcggcgc accacgccgg catgctcccg gtgttcaagg
     8941 agacggtcga ggagctgttc gaggccgggc tggtcaagat cgtgttcgcc accgagacgc
     9001 tgtcgctcgg gatcaacatg ccggccaggt cggtggtgat cgaggacctg tggaagttcc
     9061 agggcgagcg gcacgagttg ctcacaccgg gcgagtacac gcagctgacc gggcgggcgg
     9121 gacgccgggg gatcgacgag ctcgggcacg ccgtggtcgt cgtccagcgc aacgtcgcgt
     9181 tcgaacgtgt ggcctcgctc gcgtcgaccc ggacctacga gctcacgtcg tcgttccggc
     9241 cctcgtacaa catggccgtg aacctggtcc gcaactacac gcgggacgag gcgcaccacc
     9301 tgctgaactc ctcgttcgcg cagttcctcg cggaccgcgg cgtggtgacc ctcgagcgcg
     9361 agctcgaacg cgatcgggct caggtcgccg ggttccgcga gcggatgcgg tgccacctgg
     9421 gcgacttcgc cgagtactgg gcgctccgcg agaaggcgat ggccgtccgg gacgagttcc
     9481 gcaagggccg cgaacgggcg cgcaccgagt cgatccggga gggcctcgcg gccctccgtc
     9541 cgggcgacgt gatcttcgtg cctcgcgcgc gccgccgggg gctcgccgtc gtgctgtccg
     9601 cccgagaggg gaggcccacg gtgctcgcgc aggaccgcac gttcttccgg ctggggttgc
     9661 gggacttcca ggagccgccc acggtcctca cgaaggttcc gctgccgcgg tccggaagcg
     9721 ttcggagcgc ccgctaccgc cgtgacctgg cggcgcggct cgtcgcgctg gacgtccgcc
     9781 ccccacgcaa gcctcagggc cgcgtcgatg cgcgggtgga gcgcgaggcg gccaggtacg
     9841 agcgccttgc ggaggcgcac ccgtgccacg cgtgtcccga ccggcccgag cacgagcggt
     9901 gggcgaccag ggcctcgcag gtcgaacacc agatccgcgg gctcgaccgc cgcatccgaa
     9961 cccgaaccga gacgctcgcc cggcagttcg aacgcgtgat cgcggtgctc gaggagctcg
    10021 ggtacctacg ttcgttcgcg atcgagccca aggggcggac gctggcgcgg atctacggcg
    10081 agggcgacat cctcatggcc gaggcgctcg cagacgggct cctcgagggc ctggacccct
    10141 ccgaggtggc cgcggtggtc tcgaccgtcg tgtacgagtc gcgtgaacgc acaccccggg
    10201 ccgccgagat gcccacgccc gcctcggtcg agcggtacgc ggcgttccgg aggatctggc
    10261 gccgggtccg cgaagccgag gaccgccacc aggtggagct ctgccgcgag ctcgaggacg
    10321 ggttcgcggc gccgatccat cggtgggcgc agggggagtc gctcgaggac gtcctgcgcg
    10381 agacggacat gtcgccgggg gacttcgtgc gcacctgcaa gcaggtgctc gacctgct
//
LOCUS       DATBRK010000428         4281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_103589, whole genome shotgun sequence.
ACCESSION   DATBRK010000428 DATBRK010000000
VERSION     DATBRK010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103589"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /gene="hemB"
                     /locus_tag="VIH70_12710"
     CDS             <1..674
                     /gene="hemB"
                     /locus_tag="VIH70_12710"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY8117270.1"
                     /translation="RVLREELGDDHVVIADLCLDEYTDHGHCGVLDEHEQVDNDATLE
                     RYRRIATAQAEAGAHLVGPSGMMDGQVAAIRDELDAGGFEGVGILAYAAKFASAFYGP
                     FREAAECAPRFGDRTGYQMDPPNADEAIREIEADIEQGADVVMVKPALPYLDVIRRAK
                     DRTRFPMAAYNVSGEYAMLKAAAQSGWLDERGAVLEVLTSIRRAGADLILTYHARDAA
                     DWLST"
     gene            772..1011
                     /locus_tag="VIH70_12715"
     CDS             772..1011
                     /locus_tag="VIH70_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY8117271.1"
                     /translation="MRVHISLDEELVRELDELVGSRGRSPFIADAVRARVDQERRWRT
                     IRAAIGSIADHGHPWDDDVAKWVHDSRREDPGRVG"
     gene            1011..1406
                     /locus_tag="VIH70_12720"
     CDS             1011..1406
                     /locus_tag="VIH70_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8117272.1"
                     /translation="MTFVLLDTTVLIDVLEERPLTTERFLDLQPRGDVPHVCAITVEE
                     VSAWLRPKERERASTMFEGLSMAPLGIAEGRLAGWWRRRYRRSGRTLTQADALIAAAA
                     VGIGATLVTGNPKDFPMEVLVVEHWPAGE"
     gene            1436..2710
                     /gene="hemL"
                     /locus_tag="VIH70_12725"
     CDS             1436..2710
                     /gene="hemL"
                     /locus_tag="VIH70_12725"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY8117273.1"
                     /translation="MTRSEDLFERAQRAIPGGVNSPVRAFGAVGGTPRFVVRGEGAYV
                     VDADGNRYIDLVQSWGALLFGHARSEIVEAARAAAEHGTTFGAPTEAEVELAERITAA
                     MPSIEMVRLVSSGTEAAMSAIRLARGFTGRDLLVKFEGCYHGHSDALLSSGAGSGVAT
                     LGIPGSPGVTEGAARDTITLTYNDVETLDEAVGRHGEAIAAVIVEPVAANMGVVPPGP
                     GFLERLREVCNASGALLVFDEVITGFRIAFGGAQGVYAVTPDLTILGKVMGGGFPCAA
                     FGGRREVMERLAPVGPVYQAGTLSGNPVAVAAGIAALGLAEELDPYPALTETATALAD
                     GLTERIDARGEPVTVNRAGSMLSLFFSTGPVRDFADAKASDHQHYARFFHHMLDNGVW
                     LPPSGYELWTLGTEHGPAEVEHVLGAASSFEG"
     gene            complement(2770..>4281)
                     /locus_tag="VIH70_12730"
     CDS             complement(2770..>4281)
                     /locus_tag="VIH70_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8117274.1"
                     /translation="NADFASEALGESALAGVARNRIVGYAEPALREKVDEVESLVSAN
                     GVGPTIEAAADAEPLADLQWDMAMIHATVDESYAVGTGNHDVLVGIIDTGIDASHPDL
                     APNFNADLSRNFTTDIPLIDGPCNQEPDHSCQDPPDVDEGGHGSHVAGTVGAALNGLG
                     IAGVAPDVSLVNLRAGQDSGFFFLFETLAALTYAADNGIDVVNMSFFTDPWLFNCRAN
                     ELDTPTEQQEQATIIDATNAALDYAHANGVTLIAAEGNEHTDLGNPTDDAISPDYPPG
                     TEKVRDNITNDCLIIPTEGNHVLSISSLGSTGRKAYYSNYGLEQTVVSAPGGDRRQFF
                     GTPQYNTAGLRVLSTYPAELAMEEKLITRNFKPRTPLAVVDCGGGNPSPSTCGVYVYL
                     QGTSMASPHAVGVAALIIAAIGSGTGADFGADPDEVEAMLRSTATNADVFFTAFSGDD
                     WRDFCPVPPTLFHYDDTTLPVDPPDWDALCEGDADFNGFYGDGVVDAEEAASL"
BASE COUNT          656 a         1419 c         1513 g          693 t
ORIGIN      
        1 tccgcgtgct gcgggaggag ctcggcgacg accacgttgt gatcgccgac ctgtgcctgg
       61 acgaatacac ggaccacggc cactgcggcg tgctcgacga gcacgagcag gtcgacaacg
      121 acgcgacgct cgagcgctac cggcggatcg cgaccgcgca ggccgaggcc ggggcccatc
      181 tcgtcggacc gagcggcatg atggacggcc aggtggccgc gatccgcgac gagctcgacg
      241 cgggcgggtt cgaaggcgtg gggatcctgg cgtacgcggc gaagttcgcc tccgcgttct
      301 acggcccgtt ccgcgaggcc gccgagtgcg ctccgcgctt cggcgaccgc accggctacc
      361 agatggaccc gccgaacgcc gacgaggcga tccgcgagat cgaggccgac atcgagcagg
      421 gcgccgacgt cgtgatggtc aagccggcgc ttccctatct cgacgtgatc cgccgtgcca
      481 aggaccggac gcggttcccg atggccgcgt acaacgtctc gggcgagtac gcgatgctga
      541 aggcggccgc ccagagcggg tggctcgacg aacgcggcgc ggtgctcgag gtgctcacgt
      601 cgatccggcg cgcgggcgcc gacctgatcc tcacgtacca cgcgagggac gccgccgact
      661 ggctgtcgac ctgaggaccg tcagcccagg tcgtcctcga tccgatatcc gcgatccggc
      721 gaccgagtcg gacgctctgc gtggaaggtt agtgtgccat cgtgtgtgcc gatgcgtgtg
      781 catatcagcc tcgatgaaga gctcgtccgt gagctcgacg agctggtcgg ctcacgaggt
      841 cgaagcccgt tcatcgccga cgcggtgagg gcgagggtcg atcaggaacg gcggtggcga
      901 acgatccgtg cggcgatcgg gtccatcgcc gatcacggtc acccctggga cgacgacgtc
      961 gcgaagtggg ttcacgattc gcggcgtgag gatccgggac gcgtcggctg atgaccttcg
     1021 tcctgctcga tacgacggtc ctcatcgatg tcctcgagga gcgaccgctc accaccgagc
     1081 ggttcctcga cctacaacct cgaggcgacg ttcctcatgt ctgtgccatc acggtcgagg
     1141 aggtgtctgc gtggctgcgt cccaaagaac gcgagcgtgc ctccacgatg ttcgagggtc
     1201 tgtcgatggc acctctcggg atcgccgaag gacggctcgc cggatggtgg cgacgccgat
     1261 atcgaaggtc tggtcgcacg ctgacgcaag cggatgccct gatcgcggcg gctgcggtag
     1321 ggatcggggc gacgctcgtc acgggcaatc ccaaggactt cccgatggaa gtcctcgtcg
     1381 tggaacactg gcctgccggc gagtgatccg accgcctcgc taggcttggc ccgagatgac
     1441 tcggtccgag gacttgttcg agcgcgccca gcgcgcgatc cccggcgggg tgaactcgcc
     1501 cgttcgcgcg ttcggcgcag tgggcgggac cccgcggttc gtggttcggg gcgagggcgc
     1561 gtacgtcgtc gacgccgacg gcaaccggta catcgacctc gtccaatcct ggggcgcgct
     1621 cctgttcggt cacgcgcggt ccgagatcgt ggaggcggcg cgtgcggccg cggagcacgg
     1681 gaccacgttc ggcgccccca ccgaagccga ggtcgagctc gccgaacgca tcacggcggc
     1741 gatgccctcg atcgagatgg tccggctcgt ctcgagcggg accgaggccg ccatgagcgc
     1801 gatccgcctg gcgcgggggt ttaccgggcg cgatctcctg gtgaagttcg aaggctgtta
     1861 ccacggacac tcggatgcgc tgctctcgtc cggcgccggg agcggcgtgg cgacgctcgg
     1921 gatccccggc tcgccgggcg tgaccgaggg agcggcgcgc gacacgatca ccctgaccta
     1981 caacgacgtc gagacgttgg acgaggccgt cgggcgccac ggcgaagcga tcgcggcggt
     2041 catcgtcgaa cccgtcgcgg ccaacatggg cgtcgtgccg cccgggcccg ggttcctcga
     2101 acgcctgcgg gaggtctgca acgcgagcgg cgcgctgctc gtgttcgacg aggtgatcac
     2161 cgggttccgg atcgcgttcg gcggcgcgca gggcgtgtac gcggtgacgc cggacctcac
     2221 gatcctcggc aaggtcatgg gtggtgggtt cccgtgcgcg gcgttcggcg gccgccgtga
     2281 ggtgatggag cgtctcgcgc ccgtcggccc cgtctaccag gccggcacgc tgtccgggaa
     2341 ccccgtcgcg gtcgcggccg gcatcgcggc actcgggctg gccgaggagc tcgacccata
     2401 tccggcactc acggagaccg ccaccgccct cgccgacggg ctgaccgagc gcatcgacgc
     2461 ccgcggcgag ccggtcacgg tgaaccgggc cggatcgatg ctcagcctct tcttctcaac
     2521 ggggcctgtg cgtgacttcg cggatgcgaa ggcctcggac caccaacact atgcccgctt
     2581 cttccaccac atgctcgaca acggggtgtg gctgcccccg agcggctacg agctgtggac
     2641 cctgggaacc gagcacggcc cggcggaggt cgagcacgtt ctgggcgccg cgtcctcgtt
     2701 cgaagggtga gacggagacc gccccggggg ttggccgggg cggtctcgaa acgaacgtgc
     2761 aggatcgggc tacaagctcg ccgcctcctc ggcgtccacg acaccgtccc cgtagaagcc
     2821 gttgaagtcc gcatcgcctt cgcagagggc gtcccagtcc ggcggatcca ccggcagcgt
     2881 tgtgtcgtcg tagtggaaca gggtcggcgg caccgggcag aagtctcgcc agtcatcgcc
     2941 actgaacgcc gtgaagaaga catcggcgtt cgtcgcggtg ctccggagca tggcctccac
     3001 ttcatcggga tcggcgccga agtcggctcc cgtaccactt ccgatcgcgg cgatgatcag
     3061 cgcggcgaca ccgacggcat gaggcgatgc catcgaggtc ccctgcaagt acacgtagac
     3121 gccgcaggtg ctcgggctgg gattgccccc gccacaatcg acgaccgcga gcggcgtcct
     3181 gggtttgaag ttccgcgtga tcagcttctc ttccatcgcg agctccgccg ggtacgtcga
     3241 caggacacgg agcccggcgg tgttgtactg aggggtaccg aagaactggc gacggtcgcc
     3301 gcccggcgcg gacacgaccg tctgctcgag cccgtagttg gagtagtacg ccttgcggcc
     3361 cgtcgagccg agcgagctga tcgagagcac gtggttgccc tcggtgggga tgatgaggca
     3421 gtcattcgtg atgttgtccc gaaccttttc ggtacccggc gggtagtcgg gactgatcgc
     3481 atcgtcggtt gggttcccca gatccgtgtg ctcgttcccc tcggctgcga tcaacgtcac
     3541 gccgttggcg tgcgcgtagt ccaacgcggc gttggtcgca tcgatgatcg tcgcctgctc
     3601 ctgctgctcc gtcggcgtat ccagctcgtt ggcgcggcag ttgaacagcc aggggtccgt
     3661 gaagaaactc atgttgacca cgtcgatgcc gttgtcggcc gcgtacgtga gcgcggcgag
     3721 ggtctcgaag aggaagaaga aaccggaatc ctgtccggcg cggaggttga ccaggctcac
     3781 gtccggcgcg accccggcga tacccaggcc gttgagtgcc gcgccgacgg tgccggcgac
     3841 gtgtgagccg tgaccgccct catcgacgtc gggcggatcc tgacacgaat gatcaggttc
     3901 ctggttgcac ggcccatcga tcagagggat gtcggtggtg aagttccggc tgaggtccgc
     3961 gttgaagttc ggcgccagat cgggatgcga cgcatcgatg ccggtgtcga tgatccccac
     4021 cagcacgtcg tggttcccgg tccccaccgc gtacgactca tcgacggtgg cgtggatcat
     4081 ggccatatcc cattggaggt cggcgagcgg ctccgcatcg gccgcggcct cgatcgtcgg
     4141 ccccaccccg ttcgccgaga cgagggactc gacctcgtcg accttctccc ggagcgccgg
     4201 ttcggcgtac ccgacgatcc ggttgcgcgc gacccccgcc agggccgact cgcccagagc
     4261 ctcggacgcg aagtcggcgt t
//
LOCUS       DATBRK010000429         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2263941, whole genome shotgun sequence.
ACCESSION   DATBRK010000429 DATBRK010000000
VERSION     DATBRK010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2263941"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..571
                     /locus_tag="VIH70_12735"
     CDS             251..571
                     /locus_tag="VIH70_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8117275.1"
                     /translation="MMTVNVVVLAGTVAADPVERRMPSGDEVTELRLSVPEAGKRLLP
                     LPVAAWHKDVGKRALKPIAKGDDILVYGQLVRRFYRSGAGARSLTEVVAAGIKKLEPA
                     EAAD"
     gene            complement(581..1762)
                     /locus_tag="VIH70_12740"
     CDS             complement(581..1762)
                     /locus_tag="VIH70_12740"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY8117276.1"
                     /translation="MSLVSPFVGLLFDGSIVGSHDLVTTPPYDVISDDERRHYLDASR
                     YNVIRLVLGPDDAGDGGTVDKYRQAASKLQTWRERRALVPTERPFLYPYEMRFSLHGR
                     RRAIRGMVCAVQLEDPGGSIVPHERTMPGPIEDRLLLMRAVRTNLSSIHAVFRGPSEP
                     FAGFLDDAMARGPAASTTDEAGVEHRLWVSDPNPEVARWLAPESLMIADGHHRYSMAL
                     RYRDEMRAEHGPGPWDRVMMFIADAALQQPPVLPFHRIQTGGPHEATGTRVRDLAEVL
                     ESVSDEELVYGSASLEDGLLVHRIAELAGAPPTVCALHERVLGQVDAELRFTPDAVEA
                     EDAVRKREAIAAFFLPPTDAARIRDVIDRGERLPRKSTFFWPKPRTGLVLRPLDLDAT
                     A"
     gene            1768..2154
                     /locus_tag="VIH70_12745"
     CDS             1768..2154
                     /locus_tag="VIH70_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117277.1"
                     /translation="MYSSSPRSDPPGEGAYELFSRGMGFLRDGHPHQAAMLLGRAKLI
                     EPDKASIREALGRALYMSGRTVRARREFAKVVQIEPASDYAHFALALACERTGQRTRA
                     IGHLKLAIAMRPGTGDYERALERLAG"
     gene            2154..2930
                     /locus_tag="VIH70_12750"
     CDS             2154..2930
                     /locus_tag="VIH70_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEY8117278.1"
                     /translation="MLADRYGCILFDLDGVLYRGEDAVPQAPPTLAELRRRGVRPVFL
                     TNNSSRTPRQVADKLRTIGIEADPSEVVTSALATAELLAERGGGRAFVIGQDGVREAL
                     TDAGIRILQGEPEEADLVVVGFDGGATYGSLKRASLLVQRGARLVATNADGSYPAADG
                     LWPGAGALLCVITTTTGAEPEIVGKPFHPLFEAGRRRGGGGRPLVVGDRLDTDIEGAA
                     RLGWDTMLVLTGVSSREDVERTGIRPTVIAEDVSALLEGR"
     gene            3057..3428
                     /locus_tag="VIH70_12755"
     CDS             3057..3428
                     /locus_tag="VIH70_12755"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY8117279.1"
                     /translation="MAVGLEQVRQYMEAAIGKLSPTKAQELAKSLSKSQGRDQVSKAA
                     KDLLAWSNKNKERLTSMVQTEVKSQLKTIGVASRDEVDALRKRVRELERGQGKKSTRK
                     RSTAKRSTAKPATSSPPSEQA"
     gene            3440..>3548
                     /locus_tag="VIH70_12760"
     CDS             3440..>3548
                     /locus_tag="VIH70_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY8117280.1"
                     /translation="MARRRLDAELVRRGLVATRSQAQSAVRDGLVLVSGR"
BASE COUNT          535 a         1212 c         1322 g          479 t
ORIGIN      
        1 gccgttccaa ccccgcgacc acgggctcga aggaggtgct cggtccggtt tcgacgatcg
       61 cgggctcgtc ggcgtccagg agataggcgg cggtgaagcg ttcgcggccg ccgtagaagg
      121 tgtcgatcgc ggtgatccct cgcgcgacct cgaacacgac gggctccacg cggcgagtct
      181 acgtggcgcg tcctcgggcg gtggcgtgtc gtcgggcggt gctacggtcc gacgcgaagg
      241 ggggagttgg atgatgacgg tcaacgtcgt ggtcctcgcg ggcaccgtgg ccgccgatcc
      301 ggtcgaacgc cgcatgccgt cgggtgacga ggtgaccgag ctccgactct ccgttccgga
      361 agcggggaag cgtcttctcc ctctccccgt cgcggcctgg cacaaggacg tcggaaagcg
      421 cgccctgaag ccgatcgcca agggcgacga catcctggtc tacggtcagc tcgtccgccg
      481 cttctaccgg agcggagccg gggctcgcag cctcaccgaa gtggtcgccg cgggcatcaa
      541 gaaactcgag ccggccgagg cggccgactg agatccaggg tcaggcggtc gcgtcgaggt
      601 cgagcggccg gagcacgagg cccgtgcgcg gcttgggcca gaagaacgtc gacttgcggg
      661 gcagccgttc gccccgatcg atcacgtcgc ggatccgcgc cgcgtcggtc gggggcagga
      721 agaacgcggc gatcgcctcc cgcttgcgaa cggcgtcctc tgcctcgacc gcgtcgggcg
      781 tgaaccgcag ctccgcgtcg acctgcccca ggacccgctc gtggagcgcg cacaccgtgg
      841 gaggcgcgcc ggcgagctcc gcgatgcggt ggacgaggag cccgtcctcg agggacgcgg
      901 agccgtagac gagctcctcg tcgctcacgc tctcgaggac ctcggcgaga tcgcggaccc
      961 gggtgccggt cgcctcgtgc gggccgccgg tctggatccg gtggaacggg agcacggggg
     1021 gctgctggag cgccgcgtcc gcgatgaaca tcatcacgcg gtcccacgga cccgggccgt
     1081 gctccgcccg catctcgtcc cggtaccgca gggccatcga gtagcggtga tggccgtcgg
     1141 cgatcatcag ggactcgggc gcgagccacc tcgcgacctc cgggttcggg tcgctcaccc
     1201 agagcctgtg ctcgacgccg gcctcgtcgg tggtggacgc ggcgggtccc cgcgccatcg
     1261 cgtcgtccag gaacccggcg aacggttcgc tcggaccgcg gaacacggcg tggatgctcg
     1321 acaggttcgt ccggaccgcg cgcatcaaca ggaggcggtc ctcgatcggc cccggcatcg
     1381 tgcgctcgtg cggcacgatc gagccgcccg ggtcctcgag ctgcaccgcg cacaccatgc
     1441 ctctgatcgc gcggcgccgg ccgtgcagcg agaaccgcat ctcgtacggg tagaggaacg
     1501 ggcgctcggt cgggacgagc gcacgccgct cgcgccacgt ctggagcttc gacgccgcct
     1561 gcctatactt gtcgaccgtt ccgccatcgc cggcgtcgtc cggtccgagg acgaggcgga
     1621 tcacgttgta acggctcgcg tccaggtagt gccgccgctc atcgtcggag atgacgtcgt
     1681 agggcggggt cgtcaccagg tcgtgcgagc cgacgatcga gccgtcgaac agcaggccga
     1741 cgaagggcga gaccagggac atgcaccatg tatagcagca gccctcgcag cgacccgccc
     1801 ggggagggtg cctacgagct cttctcgcgc ggcatggggt tcctccgcga cggacatccc
     1861 caccaggccg ccatgctcct ggggcgcgcc aagctgatcg agcccgacaa ggcgtcgatc
     1921 cgtgaggccc tgggccgggc gctctacatg tccggccgga ccgttcgcgc gcggcgcgag
     1981 ttcgccaagg tcgtgcagat cgagcccgcg agcgactacg cgcacttcgc gctggcgctc
     2041 gcgtgcgaac gcacggggca gcgcacgagg gcgatcgggc acctgaagct cgcgatcgcc
     2101 atgcgcccag ggacgggaga ctacgagcgc gccctcgaac gcctcgcggg ctgatgctcg
     2161 ccgaccgtta cggctgcatc ctgttcgacc tcgacggtgt cctgtatcgg ggggaggacg
     2221 cggtgcccca ggcgccgccc accttggccg agctccggcg acgcggcgtc cgccccgtct
     2281 tcctgaccaa caactcctcc cggacgccgc gccaggtggc ggacaagctg cgcacgatcg
     2341 ggatcgaggc ggacccgagc gaggtcgtga cctcagcgct cgcgaccgcc gagctcctcg
     2401 cggaacgggg aggggggcgc gcgttcgtga tcggccagga cggcgttcgt gaggcgttga
     2461 ccgacgccgg catccggatc ctccagggcg agcctgagga ggccgacctg gtcgtggtcg
     2521 gcttcgacgg cggcgccacg tacgggtcgc tcaagcgggc gtcgctgctg gtccagcggg
     2581 gggcgcgtct ggtcgccacg aacgcggacg gctcctaccc cgcggccgac ggcctgtggc
     2641 ctggtgccgg cgcgctcctg tgcgtcatca cgacgacgac gggcgccgag cccgagatcg
     2701 tgggcaagcc gttccacccg ctcttcgagg ccggtcggcg gcggggggga ggcggccggc
     2761 cgctcgtcgt cggggaccgg ctcgacacgg acatcgaggg agcggcgagg ctcgggtggg
     2821 acacgatgct cgtgctcacg ggcgtctcga gtcgcgagga cgtggagcgc acgggtatcc
     2881 gacccacggt gatcgcggag gacgtttccg cgctgctcga gggccgctag ctcgagggcc
     2941 acaagggagg cgggcggccg cggcgctatc ctgggtcgtg gcgccgggag gggcgcccac
     3001 gtgaccgaac acccgggatc gagaacgcca ggatcgaaga cgaaggagtg atcgagatgg
     3061 cggttgggct tgaacaggtc cgccagtaca tggaggcggc gatcgggaag ctgtcgccca
     3121 ccaaggccca ggagctcgcg aagtcgctga gcaagagcca gggccgcgac caggtgagca
     3181 aggccgcgaa ggacctcctg gcgtggtcga acaagaacaa ggaacgactc acctcgatgg
     3241 tgcagaccga ggtcaagtcg cagctgaaaa cgatcggcgt cgcgtcccgc gacgaggtgg
     3301 atgcgctgcg aaagcgcgtg cgcgagctcg agcgggggca gggcaagaag tcgacgcgca
     3361 agcgctctac ggccaagcgg tccaccgcga agcccgccac gtccagccct ccaagcgagc
     3421 aggcctgacg ctgcgcgccg tggcgcgccg gcgactcgac gccgagctcg tgcgccgggg
     3481 cctcgtcgcc acccgcagcc aggcgcagag cgccgtccgc gatgggttgg tcctggtctc
     3541 gggccggt
//
LOCUS       DATBRK010000430         4956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_105142, whole genome shotgun sequence.
ACCESSION   DATBRK010000430 DATBRK010000000
VERSION     DATBRK010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105142"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VIH70_12765"
     CDS             <1..1395
                     /locus_tag="VIH70_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8117281.1"
                     /translation="FGYEVSIITPDYFVPFPDGTSLTLWGDVERDAAAIGRFSERDAA
                     AYVTFDRYFDRVARLLKDLLFVVPPNMNLRDLPKWAATAGRFRKWSGRDLHETVRLFT
                     MSAADFLDEWFEDERVKGALATQAIIGAWCGPMSPGSAYVLMHHWIGEVDGHAGAWGW
                     AKGGMGGISAAMARAAEAAGAEIRTDAEVDRVAINASGRAVGVALADGSLVRAQRVVS
                     CAHPVTTYQSLIGEERLPGDVVRDVKRYRTRSGSVKLNVALSELPAFPAWDQEGDPHR
                     GLVAVSPSIEYLERAWDDAKYGRMSEHPYVEVVFPTAHEPEGLAPMGKHLMLAFSQYG
                     PYELTEGSWDEGGRDGYAARVLKALGEFAPSLESAVEHLEVLTPRDIEERFGLIGGNI
                     MQGELTPDQLFSFRPIPGHGDYRTPVAGMYLCGSGTHPGGGVMGVPGRNAASVILRDH
                     KRGEFIGRIKNLAR"
     gene            1396..2787
                     /locus_tag="VIH70_12770"
     CDS             1396..2787
                     /locus_tag="VIH70_12770"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8117282.1"
                     /translation="MLTQLAQAVREGRISAVELVQESIARIERLDPALNAVSAVWPEE
                     ALAEASALDRRGAEGPLAGLPLLVKDNTDVAGKVTNFGSRTMLERPPSERSEITVERM
                     VAAGAIVLGRTNIPEFAFQGWTDNDVFGATRNPWGLEWSPGGSSGGSGAALAAGLVPL
                     ATGTDGGGSIRTPAAFCGLAGLKPTAGMIGRRPIPSWLEVSTQGPLAPGVEDAKLLLH
                     VMRGPARGDPSAAPAWQYGEGMPRRVVATTRSRDFGPLPPEVDEPFRAALAAIEGDLG
                     FAVEEIPTSSLFPTAGVTGGESRDDWYVTVTVEEVQFLGRSWVEAHLSDFSSIFRSEM
                     ERALGFTLDDYLAARRRRFVYTEDLDRLLGDDTVLVLPTHGYEGWLADGTLPGTGRPA
                     GPEGYNAGEFNLSGHPALSLPAGTCSNGLPFGLEIVGPRYRDDLVLEFGAAFEGVRPW
                     PTVAPGYQRFAVP"
     gene            complement(2818..4236)
                     /locus_tag="VIH70_12775"
     CDS             complement(2818..4236)
                     /locus_tag="VIH70_12775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8117283.1"
                     /translation="MTDLDVPRPARPSWWLEDALALPEFAGPDTPPLRGDTAADVAIL
                     GGGYTGMWTAWFLKEREPALDVVLLERDICGGGPSGRNGGFVNGLYDEADLLLERHGE
                     GGRRTIEAAARSIDEVGAWCEHTGVDAWYEPSGDLGVSTNPAHDASVRETVSEAGRLG
                     LADVYRPLSQDEVRERFDSPVVRTGFRVAHAATVHPARLARGLRGALVERGVRIFEGT
                     PVVRFDARRPAAETPSGVVRAAHAVVALNAWARALPDFRRSLLLRGTYIVMSAPAPER
                     LEAMRWTGGEGVYDLRTSLHYLRPTRDGRIAFGGSSYRVTGQGADSAGYGYDTRSAAE
                     LVRDFRRWFPAFDGVPLETSWGGPIDVAGLHLPFFGTLPGGVTHYGLGFTGNGVGPCH
                     LGGKILSGLALGVQDEATTLPISDGDRMRFPPEPLFSIGERVVGRAIVRRDDRLDRGR
                     PPGFVTDRLARLPRRLGYMLGP"
     gene            complement(4233..>4956)
                     /locus_tag="VIH70_12780"
     CDS             complement(4233..>4956)
                     /locus_tag="VIH70_12780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8117284.1"
                     /translation="GGSVRAADAVVGLGAWATWWKAFKPRLTLRGSYMVVTAPAPDRI
                     QALGWTGGEAIRDLRSSIHYARTTPDGRIALGLGGLQPNLARRIDHRYDYEPRYARRV
                     ADDLYRMFPSFEGVPIECAWGGPINVSGFTMPFFGTLGRGNVHYGLGFTGNGVGPSHL
                     GGKILAALALHAEDGFTRLGVVTRKPKRFPPEPFRSPGAFLVNAAVRRSDDREDAGRR
                     VGALTRAIARIPRRLGYNLGPR"
BASE COUNT          698 a         1745 c         1828 g          685 t
ORIGIN      
        1 ttcgggtacg aggtctcgat catcacgccc gactacttcg tgccgttccc ggacgggacc
       61 tcgctcacgc tgtggggcga cgtcgagcgc gacgccgccg cgatcggtcg gttcagcgag
      121 cgagacgcgg ccgcgtacgt gacgttcgac cgctacttcg atcgtgtggc gcggttgctg
      181 aaggacctgc tgttcgtcgt gccgccgaac atgaacctgc gcgatctccc gaaatgggcg
      241 gccacggccg ggcgtttccg caagtggagc ggtcgcgatc tccacgagac cgtccggctg
      301 ttcacgatga gcgcggcgga cttcctcgac gagtggttcg aggacgaacg ggtcaagggc
      361 gcgctcgcga cccaggcgat catcggcgcg tggtgtgggc cgatgtcgcc gggctccgcc
      421 tacgtcctga tgcatcactg gatcggcgag gtggacggtc acgccggcgc gtgggggtgg
      481 gcgaagggcg gtatgggcgg gatctcggcg gcgatggcgc gggccgccga agccgcgggc
      541 gccgagatcc ggaccgacgc cgaggtcgac cgggtcgcga tcaatgcgag cggccgggcc
      601 gtgggggtgg cgctcgcgga cggttcgctg gtccgagcgc agcgcgtcgt ctcgtgcgcg
      661 caccctgtca cgacgtacca gtcgctgatc ggcgaggagc gactgccggg cgacgtcgtc
      721 cgcgacgtga agcggtaccg gactcgctcg ggctcggtca agctgaacgt cgcgctctcc
      781 gagctgccgg cgttccccgc gtgggaccag gagggcgacc cgcacagggg ccttgtcgcc
      841 gtctcaccgt cgatcgaata cctcgagcgc gcgtgggacg atgcgaagta cggccggatg
      901 agcgagcacc cctacgtcga ggtcgtgttc cccacggcgc acgaacccga ggggctcgcc
      961 ccgatgggca agcacctgat gctcgcgttc agtcagtacg gcccctacga gctgaccgag
     1021 gggtcgtggg acgagggcgg ccgcgacggc tacgcggccc gggttctgaa ggctctgggc
     1081 gagttcgcgc cctcgctcga gtcggcggtc gagcacctcg aggtgctcac gccgcgggac
     1141 atcgaggaac ggttcggcct gatcggcggg aacatcatgc agggcgagct cacgccggac
     1201 caactgttca gcttccgccc gatccccggg cacggcgact atcggacgcc cgtcgcaggg
     1261 atgtacctgt gcggttcggg cacgcacccg ggcggcggcg tgatgggcgt cccggggcgg
     1321 aacgccgcgt cggtgatcct gcgggaccac aagcgcggcg agttcatcgg gcgcatcaag
     1381 aacctcgctc gctagatgct gacgcagctc gcccaggccg tccgcgaagg ccggatctcg
     1441 gcggtcgagc tggtgcagga gtcgatcgca cggatcgaac gcctcgatcc ggcgctgaac
     1501 gcggtctcgg cggtctggcc cgaggaggcg ctcgccgagg cgagcgcgct cgaccggcgc
     1561 ggcgccgagg gcccgctcgc cgggctgccg ctgctcgtca aggacaacac cgacgtggcc
     1621 ggcaaggtca cgaacttcgg ctcgcggacg atgctcgaac gcccaccgtc cgagcggagc
     1681 gagatcaccg tggagcggat ggtcgcggcc ggcgcgatcg tgctcggcag gacgaacatc
     1741 cccgagttcg cgttccaggg gtggaccgac aacgacgtct tcggagcgac gcgcaacccg
     1801 tgggggctcg agtggtcgcc gggtggctcg tcgggaggct ccggcgccgc cctggccgcc
     1861 ggcctggttc cgctcgcgac cgggacagac ggcgggggtt cgatccgaac gccggcggcg
     1921 ttctgtggtc tcgcgggcct caagcccacg gcgggcatga tcgggaggcg accgatcccg
     1981 tcatggctcg aggtctcgac acagggaccg ctcgcgccgg gtgtggagga cgcgaagctg
     2041 ctgctgcacg tgatgcgcgg cccggcgcgg ggcgatccgt cggcggcacc ggcctggcag
     2101 tacggcgagg ggatgccgag gcgcgtggtg gcgacgacgc gctcgaggga cttcggcccg
     2161 ctgccgccgg aggtcgacga gcccttccgc gcggcgctcg cggccatcga gggcgacctt
     2221 ggtttcgccg tcgaggagat ccccacgtcg tcgctgttcc ccacggcggg cgtcaccggc
     2281 ggtgagtccc gcgacgactg gtacgtcacc gtgaccgtcg aggaggtgca gttcctcggt
     2341 cgctcgtggg tcgaggcgca tctgtccgac ttcagctcca tcttccggtc cgagatggag
     2401 cgcgcgttgg ggttcacgct cgacgactac ctcgcggcac gccgtcggcg cttcgtctac
     2461 acggaggacc tcgaccgcct gctcggcgac gacacggtgc tcgtgctgcc gacccacggg
     2521 tacgagggct ggctcgcgga cgggacgctg cccggcacgg ggcgacccgc cggtcccgag
     2581 ggctacaacg ccggcgagtt caacctgagc ggtcaccccg cgctcagcct gccggcgggg
     2641 acgtgctcga acggtctccc cttcggtctc gagatcgtcg gcccccgcta ccgggacgac
     2701 ctggtgctgg agttcggcgc ggcgttcgag ggcgtccggc cgtggccgac ggtcgcgccg
     2761 gggtaccagc gcttcgccgt tccatagagc cgttccctgg agagataccg atggggttca
     2821 aggccccagc atgtacccca ggcgcctggg caggcgcgcg agccgatcgg tcacgaaccc
     2881 cgggggccgt ccccggtcca ggcgatcgtc gcgacgaacg atcgcgcgcc cgacgacgcg
     2941 ttccccgatc gagaagagcg gttccggcgg gaaccgcatc cggtcgccgt ccgagatcgg
     3001 gagcgtcgtc gcctcgtcct gaactccgag cgcgaggccc gagaggatct tgccgccgag
     3061 gtgacacggg ccgaccccgt tcccggtgaa ccccagcccg tagtgcgtga ccccgcccgg
     3121 cagcgtgccg aagaacggca ggtgcaggcc cgcgacgtcg atcgggccgc cccatgaggt
     3181 ctcgagcggc acgccgtcga acgccgggaa ccaccggcgg aaatcgcgca cgagctcggc
     3241 ggccgagcgt gtgtcgtagc cgtagccggc cgagtcggca ccttggccgg tgacgcggta
     3301 gctcgagccg ccgaacgcga tccgtccgtc gcgcgtcggc cgcaggtagt ggagggaggt
     3361 ccgcaggtcg tacacgccct cgccgcccgt ccagcgcatc gcctcgaggc gctcgggtgc
     3421 gggggcgctc atcacgatgt acgtgccgcg caggagcagc gatcgccgga agtccggcag
     3481 cgcccgcgcc cacgcgttga gggcgacgac cgcgtgtgcg gcgcggacca cgccggacgg
     3541 cgtttctgcc gccggccggc gggcgtcgaa ccgcacgacg ggtgtgccct cgaagatcct
     3601 gacgccgcgc tcgacgagcg ctccccgaag cccccgcgcg agccgggccg ggtgcacggt
     3661 cgcggcatgg gcgacccgga agcccgtccg aacgacgggc gagtcgaagc gctcgcggac
     3721 ctcgtcctgg gacaacggcc gatacacgtc ggcgagcccc agacgccccg cctccgacac
     3781 ggtctctcgg accgaggcgt cgtgcgccgg gttcgtcgaa actcccaggt cgcccgacgg
     3841 ctcgtaccag gcatcgaccc ctgtgtgctc gcaccacgcg ccgacctcgt cgatcgaccg
     3901 cgccgccgcc tcgatcgtcc gcctcccacc ttcgccgtga cgctcgagca gcaggtcggc
     3961 ctcgtcgtac agcccgttga cgaacccgcc gttgcggccg ctcggcccac cgccgcagat
     4021 gtcgcgctcg aggagcacca cgtccagtgc cggctcgcgt tccttgagga accacgcggt
     4081 ccacatcccc gtgtacccgc cgccgaggat ggcgacgtcg gcggcggtgt ctccccggag
     4141 cgggggcgtg tcgggtccgg cgaactcggg cagcgcgagc gcgtcctcga gccaccagct
     4201 cggccgggcg ggccggggga cgtcgagatc cgtcaccggg gtccgaggtt gtagccgagc
     4261 cgccgaggga tccgagcgat cgcccgagtg agggcgccca cgcgacgtcc cgcgtcctct
     4321 cggtcgtcgc tgcgacggac ggcggcgttg acgaggaaag cccccggcga gcggaagggt
     4381 tcgggcggga accgtttggg cttgcgcgtc acgaccccca ggcgcgtgaa gccgtcctcc
     4441 gcatgcaggg cgagggcggc gaggatcttg cctcccaggt gggacggccc gacgccgttg
     4501 ccggtgaacc cgaggccgta gtgcacgttg ccgcgaccga gcgtgccgaa gaacggcatc
     4561 gtgaacccgc tgacgttgat cgggccaccc cacgcgcact cgatcggcac gccctcgaag
     4621 ctcgggaaca tccgatacag gtcgtcggcc acccgccgcg cgtagcgcgg ctcgtagtcg
     4681 taccgatggt cgatccgacg cgcgaggttc ggctgcaacc cgccgagccc gagtgcgatc
     4741 cggccgtcag gcgtggtccg cgcgtagtgg atcgacgagc gcaggtcgcg gatcgcctcg
     4801 ccgcccgtcc acccgagcgc ctggatccgg tccggggccg gcgcggtgac gaccatgtag
     4861 gacccccgga gcgtgagccg cggcttgaag gccttccacc aggtcgccca cgcgccgaga
     4921 ccgaccacgg cgtcggcggc ccgcaccgag ccaccc
//
LOCUS       DATBRK010000431         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_384874, whole genome shotgun sequence.
ACCESSION   DATBRK010000431 DATBRK010000000
VERSION     DATBRK010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384874"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VIH70_12785"
     CDS             <1..184
                     /locus_tag="VIH70_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY8117285.1"
                     /translation="EIAETGPGVSKLTVTHELEGAPRLALFVGGEMESMGAGGGWSEV
                     FSDLKTLLETGRGMRS"
     gene            complement(244..660)
                     /locus_tag="VIH70_12790"
     CDS             complement(244..660)
                     /locus_tag="VIH70_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010837725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8117286.1"
                     /translation="MEQRVSLITLGVRDLERARTFYEGLGWSAHDDENNDVVFFQAGG
                     MVVALWDRGSLAADSAVTDGGGWGGITLAYNVRAPAEVDAVIEEARAAGARIGREPGE
                     TFWGGYSGVFVDPDGHPWEVAHNPGWTVNDDGTITL"
     gene            785..1975
                     /gene="dinB"
                     /locus_tag="VIH70_12795"
     CDS             785..1975
                     /gene="dinB"
                     /locus_tag="VIH70_12795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY8117287.1"
                     /translation="MADEATILHADADSFYASVEQRDDPFLRGRPVIVGAGVVLAASY
                     EAKAFGVRTPMGGALARRLCPQAVVVRPRMDAYSEASKALFEVFEDTTPFVEGLSIDE
                     AFLDVGGLRRISGTPTEIAVRLRREVLEKVGLPVTVGVARTKFLAKVASGVAKPDGLL
                     VVPTDGELAFLHPLPVERLWGVGPVTATKLRERSIETVAQVAALGESALVRMLGRATG
                     RHLHALAHNRDPRPVQVGRRRRSIGTQRALGRGSRSPDRLDTDLVALVDRLARRLRKA
                     YRVCRTVTLRLRFDDFSRATRSHTLSQATAHTPTILAVARGLLASALPLIERQGITLI
                     GITFGNLSDDRAIQLALPFERGGPSAVDETLDHVRDRFGSGAITRAVLLGRDHGPSVP
                     LLPD"
BASE COUNT          352 a          900 c          845 g          401 t
ORIGIN      
        1 cgagatcgcc gagaccggcc ccggcgtgag caagctcacc gtgacccacg agctcgaggg
       61 tgcgccacgt ctcgcgctgt tcgtcggcgg cgagatggag agcatgggcg ccggcggcgg
      121 atggagcgag gtcttcagcg acctcaagac gctgctcgag accggccgag ggatgcggtc
      181 ctagccgggc tcggtggagg ccccgcgggt cgacggatcc gcggggcctc ccacgatcgg
      241 agctcagagc gtgatcgtgc cgtcgtcgtt cacggtccag cccgggttgt gcgcgacctc
      301 ccaggggtgg ccgtcgggat ccacgaagac tcccgagtag ccgccccaga acgtctcccc
      361 cggctcccgc ccgatccgag ccccggccgc gcgcgcctcc tcgatcaccg catcgacctc
      421 cgccggcgct cggacgttgt aggcgagcgt gatccctccc caacctcctc cgtcggtcac
      481 cgcgctgtcc gccgccaggc tccctcgatc ccacagggcc accaccatgc cgccagcctg
      541 gaagaacacc acgtcgttgt tctcgtcgtc gtgggcggac catccgagcc cctcatagaa
      601 ggttcgtgcc cgctcgaggt ctcggacccc gagcgtgatc aggctgacac gttgctccat
      661 gccgatctcc tctccgaggc ggagccgttc ggaccacgat gccagatgcg accgacacgg
      721 cggtaggact gctgccacgg gtcgaggccg gctcgcgtca ctacgggatg cgaccatccg
      781 aaacgtggcc gacgaggcga cgatcctgca cgcggacgcc gactccttct atgcctcggt
      841 cgagcagcgg gacgacccct tcctgcgggg ccggccggtc atcgtcggtg ccggggtcgt
      901 gctcgcggca agctacgagg caaaggcgtt cggcgtccga acgccgatgg gtggcgcgct
      961 cgctcgcagg ctctgcccgc aggccgtcgt ggttcgcccg cgcatggacg cgtacagcga
     1021 agccagcaag gcgctcttcg aggtgttcga ggacacgacg ccgttcgtcg aggggctgtc
     1081 gatcgacgag gccttcctcg acgtgggagg gctgcgtcgg atctcgggca cgcccaccga
     1141 gatcgccgtc cggctccggc gcgaggtcct cgagaaggtg gggctgcccg tcacggtggg
     1201 cgtggctcgc acgaagttcc tcgccaaggt cgcgagcggc gtcgccaagc ccgacgggct
     1261 gctggtggtc cccaccgacg gcgagctggc gttcctgcac ccgctgcccg tcgaacgcct
     1321 ctggggtgtc ggaccggtca ccgcgacgaa gcttcgcgag cggtccatcg agaccgtggc
     1381 ccaggtcgcg gcgctcggcg aatcggcgct cgttcggatg ctcggccggg cgacggggcg
     1441 ccatctgcac gccctcgccc acaaccgaga tccccggccg gtccaggtcg gacgtcgccg
     1501 ccgatcgatc ggcacacagc gggcgctcgg ccgcgggtcg agatcacccg accggctcga
     1561 cacggacctc gtcgctctgg tcgatcggct cgcgcgcagg ctgcggaagg cttaccgcgt
     1621 gtgccgcacc gtcacgctcc ggctacggtt cgacgacttc tcgcgggcga cgcgttcgca
     1681 cacgctgtcg caggcgaccg cgcacacccc gacgatcctc gccgtggcga gggggctgct
     1741 cgcctcggcc ctccccttga tcgagcgtca ggggatcacc ctgatcggga tcacgttcgg
     1801 caacctgagc gacgaccgag cgatccagct cgcactgccg ttcgagcgcg gaggcccctc
     1861 cgccgtggac gagacgctcg accacgttcg ggaccgattc gggtcggggg ccatcacccg
     1921 cgcggtgctg ctcggtcgcg accacgggcc gtcggttccc ttgctcccgg actgagaccg
     1981 ttgctccttc cggcacgatc gtggttcccg tcagatccga attcagatcc gaaagacgaa
     2041 gtcgtcgagc cgagagtcga cgactttcga ccgaacgacg ttttggccct tcggcagatg
     2101 gcgatttccc ctatctggct tacggtcgtt gcatgcaggc gttcaccgag cgggctgacc
     2161 ggagcggcgc aactcgggtc ctttgccggg cctgtacaca ctcggagttc gcgcacggag
     2221 acaacgacga tcggcggtgt ctctactcgg attgcggttg cagccgtttt cggccctgcc
     2281 tcatagaccg ggctcggcgc gggtcgatcc ccccatcggc atccgcgccc gacagaccgg
     2341 ggggttgtag gagcggaagc tcggcgcatt tgcggctcct ccaccccccc ggctgtctcc
     2401 actcctcgac gttcccgcgg cggccgctca cctggatgac gcgcgcgcgt agatcgcccg
     2461 aacggtgtcg atcgcgtcgg cgtcctcggg gcgcttgt
//
LOCUS       DATBRK010000432         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2124588, whole genome shotgun sequence.
ACCESSION   DATBRK010000432 DATBRK010000000
VERSION     DATBRK010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124588"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..>1701
                     /locus_tag="VIH70_12800"
     CDS             272..>1701
                     /locus_tag="VIH70_12800"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8117288.1"
                     /translation="MQDRPGVWLIGAVLALPLAGLALLLAVPSTDVMWEHHPSHFLLV
                     LAVALINVALALVASEAAHRRADARLFLVSLALITSSGFLALHALATPGVLLDHPNSG
                     FAVATPIGLLLASGFAAGSAVELSRADAARILRRRTPIRGALALLLIAWAIGSLTGVP
                     FLDRPLEEEAPLAFRLLTPVGIGLYAFAAWRYLRIFRMRRRLLPLSVAVAFLLLAEAL
                     IAVAFARSWHVSWWEWHVLMAVAFAIVIVSVRTEYRREGSVTAAFGGLYLEQTLERID
                     RRSSGAIEQLMPAVRSRESLAPVLERLRREGFGSEEIVLLERAAREMARVDDLFRPYV
                     GPALADRLELEPSIAELGGREAEVTVLFADLAGFTSFSDGRSPMDVVQMLNAYWATAV
                     PIVTEREGGLIERFAGDAILVVFNALEDQPDHALRAVRAATAMQRAVTETSDDHPDWP
                     RFRIGINSGPAALGNVGAGAHRSFTAV"
BASE COUNT          204 a          608 c          594 g          295 t
ORIGIN      
        1 cttcccgcgc tcgggggttc ccaacaggac gtcgctgctt cctcgcgcca tctcgcctcc
       61 gtgttcgtcg aaccagcgat ccgtaggcga gaggcgagtc ggtccgcagg cgaggcccgt
      121 tcgatcgctc gtcgaggatc ctcgggcggc gcccgagata cataccctac tatccggagg
      181 cggacgtgcg ctccggacgt tcccgcctcg tcgcgcggac gcggccacag cacgggcgcg
      241 gcgtggcacc gcttcgatcc atggagggga cgtgcaggac cgcccggggg tctggctcat
      301 cggcgcggtg ctcgcgctcc cgctcgccgg cctcgccctg ctcctcgccg tcccgagcac
      361 cgacgtcatg tgggagcatc acccgtcgca cttcctgctc gtcctggcgg tcgcgctgat
      421 caacgtcgcg ctcgcgctgg tcgcgagcga ggcggcgcac cgacgagccg atgcgcgtct
      481 gttcctggtc tcgctggcgt tgatcaccag ctccggcttc ctcgcgttgc acgcgctcgc
      541 gacaccgggc gtcctgctcg atcatccgaa ctccgggttc gccgtcgcga cgccgatcgg
      601 cctgctgctc gcctcggggt tcgccgccgg gtccgccgtc gagctcagcc gcgcggacgc
      661 ggcaaggatc cttcggcgca gaacgccgat ccgaggcgcc ctcgcgctcc tgttgatcgc
      721 ctgggcgatc gggtcgctca ccggcgtgcc gttcctcgat cgaccactcg aggaggaggc
      781 gccgctcgcg ttccggctgc tcacgccggt ggggatcggg ttgtacgcgt tcgcggcgtg
      841 gcggtacctg cggatcttcc ggatgcggcg gcggttgctg ccgctgtcgg tcgcggtcgc
      901 cttcctgctg ctcgccgagg ccctgatcgc ggtcgccttc gcgcggagct ggcacgtgtc
      961 gtggtgggaa tggcacgtcc tgatggccgt cgccttcgcg atcgtgatcg tctcggtccg
     1021 caccgaatac cgtcgcgagg gttcggtcac cgcagccttc ggagggctct acctggagca
     1081 aacgctcgaa cgcatcgatc ggcgctcgtc cggcgcgatc gagcaactca tgccggcggt
     1141 ccgatcccgc gagtcgctcg ctcccgtcct cgagcggctc cggcgtgagg ggttcggatc
     1201 cgaggagatc gtcctgctcg agcgcgccgc gcgcgagatg gcgcgtgtcg acgatctgtt
     1261 ccgtccgtac gtcggcccag cgctcgcgga ccggctcgag ctggagccct cgatcgccga
     1321 gctcggaggg cgcgaggcgg aggtgacggt cctgttcgcc gatctcgcgg gcttcaccag
     1381 cttttcggac ggacgttccc cgatggacgt cgtccagatg ctcaacgcgt attgggcgac
     1441 cgccgttccg atcgtcaccg agcgtgaggg cgggctgatc gaaaggttcg ccggcgacgc
     1501 gatcctcgtc gtgttcaacg cgctggagga ccagcccgat cacgcgcttc gcgccgtgcg
     1561 cgccgcgacg gcgatgcagc gagcagtgac cgagacctcc gacgaccacc cggactggcc
     1621 gaggttccgg atcggcatca actcagggcc ggcggcgctc ggcaacgtgg gcgcgggcgc
     1681 gcaccggagc ttcaccgcgg t
//
LOCUS       DATBRK010000433         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_688987, whole genome shotgun sequence.
ACCESSION   DATBRK010000433 DATBRK010000000
VERSION     DATBRK010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688987"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VIH70_12805"
     CDS             complement(<1..753)
                     /locus_tag="VIH70_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8117289.1"
                     /translation="MTDVRELRELAEKRYDDFVDALREMVNVDCGSYTPEGVNAIADL
                     CQARFERGGWAVERIRHEPTAGQGRLGDLVIGRLEGGGRARVLMIGHTDTVFDAGTVA
                     ERPFRIEGDRAFGPGVTDMKGGLLTGFFAVEVLQAAGFDGFGTITYICNPDEEIGSPW
                     SRDTILAEAGRADAALVLEGARENGDVVSSRKGVLDVRLEIEGRAAHAGVEPERGRSA
                     ILEAAHKTVALQALNGRWPGVTVNVGMVHGGTR"
     gene            complement(785..>2928)
                     /locus_tag="VIH70_12810"
     CDS             complement(785..>2928)
                     /locus_tag="VIH70_12810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8117290.1"
                     /translation="APHLAEDEMFRERFIRESRIAAGLDHPNVVTIYDAGEVEDGLYI
                     SMRYVEGSDLGKILQTVGALEPSRTILILSQVASGLDAAHVQGLVHRDVKPGNVLVEG
                     PGSVLERAYLSDFGISKRTTTGSGLTRTGQFVGTVDYVAPEQITGEPIDGRTDVYSLG
                     CVLYQCLSGRVPFRADTEVATIYRQLNDAPPALEDFADADGLNRVIAKALSKSKEDRY
                     HSCGGLIEAIRGPLVGAAMTPTVVGPRIERDVPTPASEPVTDVLVKPTDHDGHPWGRV
                     AWLAAAGVILSAGIVGAVLLAGRGDGGGGGTDLGGGNGLRTNGQSTTTTPPATAPADN
                     LRLNWNLIKAPDLPGDQVMYDVAPTREGLVAVGHADGASSFDHDAAVWSSSDTERWDQ
                     SESAPLGGPGDQRAMAVTEFSSDDPEGDVLVVGGWDDLVPAIWWSTNGGARLTHSSSP
                     DLVANGGAIRDFVAFGSTLLAAGATGPLGTQDAAVWRSTDGRNWTMVDDADLVASGDQ
                     QIWSVHNAGGRLVGVGYTTEDGWYDAAVWTSRDGLDWARVDPAMFAETGAQLMKAVAG
                     GTDGMPLVAVGCQDDISKCDLGADSDAAVWISADGETWEKVGLEKEGVVGVGIQTMFG
                     LTLQGDTFIASGTASAKTGDLDAAVWTSTDGRTWNPRLHPRFLIDDLGGPPDDQAIRA
                     LTPFHRRSLSFVAVGVTEDASDQDSAVWGGT"
BASE COUNT          439 a         1073 c          971 g          445 t
ORIGIN      
        1 gcgcgtgccg ccgtgcacca tgcccacgtt cacggtcacg cccggccagc ggccgttgag
       61 cgcctggagg gccaccgtct tgtgggcggc ctcgaggatc gcgctgcggc cgcgttcggg
      121 ctcgacgccc gcgtgggcgg cgcgcccctc gatctcgagc cggacgtcga gcacgccctt
      181 gcgcgaggag acgacgtcgc cgttctcgcg ggcgccctcg aggaccaggg ccgcgtcggc
      241 ccgtcccgcc tcggccagga tcgtgtcgcg cgaccagggc gacccgatct cctcgtccgg
      301 gttgcagatg taggtgatcg tcccgaaccc gtcgaacccg gccgcctgga ggacctcgac
      361 cgcgaagaag ccggtcaaca ggccgccctt catgtcggtc acgcccggac cgaacgcgcg
      421 gtcgccctcg atgcggaagg gacgttcggc gacggtcccg gcgtcgaaca cggtgtcggt
      481 gtggccgatc atcaggaccc gagcgcgacc gccgccctcg agccgtccga tcacgaggtc
      541 gccgagccgg ccctggccgg cggtgggttc gtgcctgatc cgctcgacgg cccacccacc
      601 gcgctcgaac ctggcctgac acaggtcggc gatcgcgttc acgccctcgg gtgtgtagga
      661 gccgcagtcg acgttcacca tctcgcggag cgcgtcgacg aaatcgtcgt atcgcttctc
      721 ggcgagctct cggagttcac ggacgtccgt catcgcgcgg agtataggtt cgagcccacc
      781 ggcatcacgt cccgccccaa accgcgctgt cctggtcgct ggcatcctcg gtgacgccga
      841 cggcgacgaa cgacagactc cggcggtgga agggtgtcag tgccctgatc gcctggtcgt
      901 ccggaggccc ccccagatcg tcgatgagga acctggggtg cagccgcggg ttccacgtcc
      961 ggccgtcggt cgacgtccac acggcagcat ccagatcccc ggtcttcgcc gaggcggtgc
     1021 ccgaggcgat gaaggtgtcg ccctgcaggg tgagaccgaa catcgtctgg ataccgacac
     1081 cgaccacgcc ctccttctcg agcccgacct tctcccatgt ctcgccgtcc gccgagatcc
     1141 acaccgcggc atcggagtcc gcgcccaggt cgcacttgga gatgtcgtcc tggcaaccga
     1201 cggcgaccag cggcatccca tccgtgccgc ccgcgacggc cttcatcagc tgcgcgccgg
     1261 tctcggcaaa catcgcgggg tcgacccgcg cccaatcgag accatctctc gaggtccaga
     1321 ccgccgcgtc ataccatccg tcctcggtgg tgtacccgac accgacgagc cggccaccgg
     1381 cgttgtgcac cgaccagatc tgttggtctc ccgaagccac gagatccgcg tcgtcgacca
     1441 tcgtccagtt ccgaccatcg gtcgacctcc acacggcggc gtcctgcgtg ccgagaggac
     1501 ccgtcgctcc ggcggcgagg agcgtcgaac cgaacgccac gaaatcgcgg atcgcccccc
     1561 cgttcgcgac cagatccggc gaactcgagt gtgtcagcct cgccccgccg ttggtcgacc
     1621 accagatggc cgggaccagg tcgtcccagc ccccgacgac gaggacgtcg ccttcggggt
     1681 cgtcgctcga gaactcggtc acggccatgg cgcgctgatc gcccggacca ccgagcggcg
     1741 cggactcgga ttggtcccac cgctccgtgt cggacgagga ccacacggcg gcgtcgtggt
     1801 cgaaggagga ggcaccgtcg gcgtggccga ccgccacgag gccctcgcgg gtcggtgcca
     1861 cgtcgtacat cacctgatcg ccggggaggt ccggcgcctt gatcaggttc cagttgagtc
     1921 tcaggttgtc ggcaggcgcc gtcgcgggag gggtcgtggt cgtcgattgg ccgttcgtac
     1981 gcaggccgtt gccgccaccg agatccgtgc cgccgccacc accatcgccg cggcccgcga
     2041 gcaggaccgc cccgacgatc cccgcggaaa ggatgacgcc ggcggcggcg agccaggcga
     2101 cgcggcccca ggggtggccg tcgtggtccg tcggcttgac cagcacgtcc gtgacgggtt
     2161 cggaagccgg ggtcggaacg tcccgctcga tgcgcggccc caccaccgtc ggcgtcatgg
     2221 cggctccgac gaggggaccc cggatggctt cgatcagccc gccgcacgag tggtaccggt
     2281 cctccttcga cttcgacaac gccttggcga tcacgcggtt caggccgtcc gcgtccgcga
     2341 agtcctcgag cgcgggaggc gcgtcgttca actgccggta gatcgtggcg acctcggtgt
     2401 ccgcgcggaa ggggacccgc ccggagaggc actggtacaa cacgcagccg agcgagtaga
     2461 cgtcggttcg cccgtcgatc ggctcgcccg tgatctgctc cggcgccacg taatcgaccg
     2521 tgcccacgaa ctgtcccgtc cgcgtcaacc cggagcccgt cgtggtgcgc ttggagatgc
     2581 cgaaatccga gaggtacgca cgctctagca cgcttccggg gccttccacc agcacgttgc
     2641 ccggcttcac gtcccgatgg acgaggcctt gcacgtgcgc cgcatcgagc ccgctggcga
     2701 cctgcgagag gatcaggatc gtccgcgacg gctcgagggc acccacggtc tgcaggatct
     2761 tgcccagatc gctcccctcg acgtaccgca tcgagatata gagaccgtcc tccacctcgc
     2821 cggcgtcgta gatcgtcacg acgttgggat gatcgagccc tgcggcgatc cgcgattcgc
     2881 ggatgaatcg ctcacggaac atctcgtcct cggccaggtg cggagcca
//
LOCUS       DATBRK010000434         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2703063, whole genome shotgun sequence.
ACCESSION   DATBRK010000434 DATBRK010000000
VERSION     DATBRK010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703063"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VIH70_12815"
     CDS             <1..319
                     /locus_tag="VIH70_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8117291.1"
                     /translation="EAKNRRRSVGRRERLSVRLAQDRGSGDAAPSPEAAALGREEREA
                     LLGAIERLSEPERLVVTYRYLLELSEAETARALAIRPGTVKSRLSRALARLRDQLEGS
                     GDG"
     gene            312..1079
                     /locus_tag="VIH70_12820"
     CDS             312..1079
                     /locus_tag="VIH70_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117292.1"
                     /translation="MGDRPMTTEQVASALTELSSELAYPATPSMRSAVTARLENERAA
                     GARPVFPRRALWSRRRVLVLAAIGLLAALALAAAARFAIGAIEIRVQRGVTPSASAPP
                     VTPDTLGDPLPVRDAIAIAGFEPSLPAGPAPDEAYMVDSLFGDPALLFAWRPSTTYPA
                     VPGTKWGLVLMAFQGDDETVVKSVQAFEDVHPARVDGAPAAWIPVPHVLELETERGFQ
                     SFSVRGNVLIWQVDDVTYRMETSLDRASATAVAQSAG"
     gene            1141..1704
                     /locus_tag="VIH70_12825"
     CDS             1141..1704
                     /locus_tag="VIH70_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY8117293.1"
                     /translation="MARRNAIVMTAAILAALVVPAVPAAAGGGCHSGVTQGTGDTVEL
                     VDACFTPTTLQIQPGGSVTFVNTDPMVHNVGGNLWGHFDDLDPGQAFSATFDEPGVYP
                     YACWYHPGMTGAIVVGDGTGVGNGESVTVASFQQPEPSPVVEIRTVTRQASSTPVVTG
                     WVVGSLLGIGIGLGIATLVRRRSRPAA"
     gene            complement(1708..>2170)
                     /locus_tag="VIH70_12830"
     CDS             complement(1708..>2170)
                     /locus_tag="VIH70_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8117294.1"
                     /translation="RDTETVRTSLQAAETGHLVLSTLHTVDATETVNRVVDFFPPEQQ
                     GQMRLTLAGALKGIICQRLVSARDGGMIPCHEILVNTGRVAERISDSDKTAEIHEVIA
                     EGGYYGMCTFDQSLLQLVKDEKVTVEAALSASSNPHDFTLQLQQARIPLPS"
BASE COUNT          285 a          783 c          789 g          313 t
ORIGIN      
        1 cgaggcgaag aaccggcggc ggtcggtggg ccggcgcgag cggctctcgg ttcggctcgc
       61 gcaggaccgc ggctcggggg acgcggcccc gtcccccgag gccgcggccc tggggcgcga
      121 ggaacgggag gcgttgctcg gggccatcga gcgcctctcg gagccggagc ggctcgtcgt
      181 cacgtaccgc tacctgctcg agctctcgga ggccgagacc gcccgagcgc tggcgatccg
      241 gcccggaacc gtgaagtcgc ggctctcgcg cgccctcgcg cgactgcgcg accagctgga
      301 gggatccggg gatgggtgat cgaccgatga cgacggagca ggtggcctcg gcgctcacgg
      361 agctgagctc cgagctcgcg taccccgcga ccccatcgat gcggagcgcg gtcaccgccc
      421 gactggagaa cgagcgcgcg gccggggcca ggcccgtgtt cccccgtcgc gcgctctgga
      481 gccggcggcg agtgctcgtg ctcgcggcga tcgggctgct ggcggcgctc gcgctcgccg
      541 ccgccgctcg gttcgcgatc ggcgcgatcg agatccgcgt ccagcgcggc gtcacgccct
      601 cggcgtcggc cccgccggtg acgccagaca cgctcggcga ccccctcccc gtccgggacg
      661 cgatcgccat cgcggggttc gagccgtcgc tgcccgccgg acccgccccc gacgaggcct
      721 atatggtcga cagcctgttc ggcgaccccg cgctgctctt cgcctggcgg ccgagcacca
      781 cgtacccggc ggtgcccgga acgaagtggg gcctcgtgct gatggcgttc cagggtgacg
      841 acgagacggt cgtcaagagc gtgcaggcgt tcgaggacgt tcaccccgcc agggtggacg
      901 gcgcccccgc tgcgtggatc cccgtgccgc acgtgctcga gctcgagacc gagcgcggct
      961 tccagtcgtt ctcggtccgc ggcaacgtcc tgatctggca ggtggacgac gtgacctacc
     1021 ggatggagac ctcgctcgac cgcgcctcgg cgaccgcggt cgcccagtcg gccggctgag
     1081 ggaaccgacc ccggaccggc ggtgtacagg ttccggaacc cgagcgggag aggagacgtc
     1141 atggcccggc gcaacgcgat cgtgatgacc gccgccatcc tcgccgccct ggtcgtcccc
     1201 gccgtgccag ccgccgccgg cggcggatgc cacagcggcg tgacgcaggg cacgggagac
     1261 acggtggagc tggtcgacgc gtgcttcacc ccgaccacct tgcagatcca gccggggggc
     1321 tccgtcacct tcgtgaacac cgacccgatg gtccacaacg tgggcggcaa cctgtggggt
     1381 cacttcgacg acctcgaccc gggtcaggcc ttctcggcca cgttcgacga gccgggcgtc
     1441 tacccgtacg cgtgctggta ccacccgggc atgaccggtg ccatcgtggt cggagacggc
     1501 acgggcgtcg gcaacggcga gtccgtcacg gtcgcgtcgt tccagcagcc cgagccttcg
     1561 ccggtggtcg agatccgaac cgtcacccgg caagcgagct cgaccccggt cgtgacggga
     1621 tgggtggtcg gctcgctgct cgggatcggg atcggcctcg gcatcgccac gctggtccgg
     1681 cgccggtcgc ggccggccgc ctgaccgcta gctcggcagc gggatcctcg cctgctggag
     1741 ctgcagggtg aagtcgtggg ggttcgacga ggcggagagc gccgcctcga ccgtgacctt
     1801 ctcgtccttg acgagctgga gcaggctctg gtcgaacgtg cacatcccgt agtacccgcc
     1861 ctcggcgatc acctcgtgga tctccgcggt cttgtcggag tcggagatcc gttcggcgac
     1921 ccgtccggtg ttcaccagga tctcgtggca ggggatcatc ccgccgtcgc gggcggagac
     1981 cagccgctga cagatgatcc ccttcagcgc cccggccagc gtgagccgca tctgtccctg
     2041 ctgctcgggc gggaagaagt cgacgacgcg gttcacggtc tcggtcgcgt ccacggtgtg
     2101 gagcgtcgac aagaccaggt ggccggtctc ggccgcctgc agcgacgtcc gcacggtctc
     2161 ggtgtcccgc
//
LOCUS       DATBRK010000435         3832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_387307, whole genome shotgun sequence.
ACCESSION   DATBRK010000435 DATBRK010000000
VERSION     DATBRK010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387307"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VIH70_12835"
     CDS             complement(<1..183)
                     /locus_tag="VIH70_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117295.1"
                     /translation="MAALVLVALASCTGAQSEEGLTPPSASPSAGNVSSSPDSSAEFF
                     GRITDCEGLRVPRVELD"
     gene            complement(898..1188)
                     /locus_tag="VIH70_12840"
     CDS             complement(898..1188)
                     /locus_tag="VIH70_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY8117296.1"
                     /translation="MATQTRGYTATKQQLLDRLARIEGQVRGVAKMVEEDRYCIDVVT
                     QINAARAALDKVGLGLLDGHVRHCLIGGHGGPTDPDEQAQELMGAVGRMVSR"
     gene            1314..>3832
                     /locus_tag="VIH70_12845"
     CDS             1314..>3832
                     /locus_tag="VIH70_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY8117297.1"
                     /translation="MTEAEILVTAGGAALIAALGWFFFGPKQARRAEVRGNVQEVQID
                     VKGGYSPNLIRVHQGVPLRLVFDRHENSECSSRVVFPDFGVSKSLAAFGRTTVELMPA
                     QAGAFGFACGMNMLHGTLIVEPDGAVPEAPEQPSIETHRHEAARAVGVGPTHVVGDTQ
                     RVEFALRADGVACPTCVTTIEGALDGLPGVDRVQANYGAERLTVDFDAAQIGIGEMRS
                     AVEGIGYRVEERTAPGSAETEDREAIERRAEIRDLSRRVIVGAILTAPVVVAVMATNV
                     FDAPWVPDWLVNRWVQLAFIAPVMFYTGWPIHRTGWLTLRHRTADMNALITIGTTAAF
                     TYSLFVTVVPSLVPEELREVYYEAVGVILTLILLGRLLEARAKAGTGEAIRTLIGLQA
                     KIARVVRNGEEREIPIGEVEVGDEILVRPGEKIPVDGEVTGGHSAVDESMVTGEPIPV
                     SKDVGDEVIGATINQTGAFRLRATKVGRDTMLAQIIKLVEAAQASKAPIQRLADLVAS
                     YFVPAAVFIAIGTFVIWFDFGPEPALTFALVSAVSVLIIACPCALGLATPLSIMVGTG
                     KGAENGVLIRSAEALETAHKLDTIVLDKTGTITKGAPALTDVAVNDGFAEEELLRLVA
                     SAERSSEHPLASAIVAGAEVRRIALVEPSEFDSVTGQGVHATVEGRSVLVGRKRLLES
                     AGVDPSALESTASRVAEEGKTAIYAAVDGRAAGVVAVADTLKADSPAAVGALRDLGLE
                     VVMITGDNARTAEAVARQVGIERVLAEVLPQDKSLEVRRLQDEGKLVAMVGDGINDAP
                     ALAQSDIGIAIGTGTDVAIEASDVTLISGELRGIVSALALSR"
BASE COUNT          616 a         1284 c         1321 g          611 t
ORIGIN      
        1 gtcaagctcg acccggggca cgcggagacc ctcgcagtcg gtgatccgtc caaagaactc
       61 ggccgacgag tccgggctag acgagacgtt accggccgaa ggagacgccg agggtggggt
      121 gaggccttcc tcgctctgcg cccccgtaca cgaggcgagc gcgaccagaa ccagcgccgc
      181 cacacacgaa gtcctcatgc ggaagagacg tcatgagcag gtcgagaggt cccccacagg
      241 gtccatcatg gatcctcccc agtcttcagc cgatcgcgcc agcgcggtcg cgcggggcct
      301 cccgctcgtt tcgacagtcc agctcatggc accaagctcc ccgctcactc ctcgaactca
      361 ccacccacat tcagaccagg tggaagcgcg taaagcatca gccggagccg atccggaaaa
      421 catcacccga agtcgcaaca cgcgcctgac ctgggctccc cgagagcttc tcatttgctc
      481 gcccttcctc gccgacacga ttcgtgtccc gcgtgtgcct tgcggtttag acggccacgt
      541 ctcggcactt ggttcgccag caggggttgc cgtggcggcg gaggcagaat cgtcactgcc
      601 ggccggatgc ggagacgcct tggtccctcg cgatcgtcaa ggtggacgca agggctttga
      661 ggccgaaagc cgacgaagcg cagggcgcag gaccgcaaag cagctcacgg aactgtttga
      721 cgaaggccag ccacccctct gagcttcgca gtcggctccg cgaaacgcgg tttgactcgg
      781 gcccaacgag cctacggccc gggccgaagg cccgctggaa cccccggttc cggcggtttc
      841 ccttttcccc ggggaaagtg gactcgggag atcgcctggc ggacgccttt ccggtgccta
      901 acgggagacc attcggccca cggcccccat cagctcttga gcctgttcgt cgggatcggt
      961 cgggccgccg tgcccgccga tcaggcagtg acggacgtgg ccgtccagca gcccgaggcc
     1021 gaccttgtcg agcgctgccc tcgcggcgtt gatctgggtc acgacatcga tgcaatagcg
     1081 gtcttcctcg accatcttgg cgacgccccg gacctgaccc tcgatcctgg ccagacgatc
     1141 caacagctgc tgcttcgtcg ccgtgtatcc gcgtgtctgg gttgccacag ttgcctcctg
     1201 ctcccttgag atttcgttga cggatgatac ccctccaggg tataggtatc aaggggaacg
     1261 gcgtcactcg atgatcggct ggtgccgcga ggcggccgaa ggggcgaacc gacgtgacgg
     1321 aagcggagat cttggtgacg gccggtggtg cggcgttgat cgcggcgctg ggctggttct
     1381 tcttcggacc gaagcaggcg cgacgagccg aggtccgcgg caatgtccag gaagtgcaga
     1441 tcgatgtcaa gggcggctac tcgcccaatc tgatccgcgt gcaccagggg gttccgctgc
     1501 gcttggtgtt cgaccgccac gagaacagcg agtgctcctc gcgagtcgtg ttccccgatt
     1561 tcggagtcag caagtcactg gccgcgttcg gcaggaccac cgtggagctc atgcccgctc
     1621 aggcgggggc gttcggcttc gcctgcggga tgaacatgct gcacggcacc ctgatcgtgg
     1681 agcccgacgg ggcggtgccg gaggctcccg agcaaccgtc gatcgagacc caccgtcacg
     1741 aggccgcgcg tgccgtcggc gtcggtccga cgcacgtcgt gggcgacacg cagcgggtgg
     1801 aattcgcgct ccgcgccgac ggggtcgcct gcccgacctg cgtgacgacc atcgaagggg
     1861 cgctcgatgg gctgcctggc gtggaccgcg tccaggccaa ctacggcgcc gagcggctaa
     1921 cggtcgactt cgacgccgct cagatcggga tcggggagat gcgctcggcc gtcgagggca
     1981 tcggctaccg cgtcgaggag cggaccgcgc ccggctcggc cgagaccgag gatcgggagg
     2041 ccatcgaacg gcgcgccgag atccgagatc tgtcgcgacg cgtgatcgtg ggagcgatcc
     2101 tgacggcgcc cgtcgtcgtt gctgtcatgg caaccaacgt cttcgatgca ccctgggtac
     2161 ccgactggct cgtgaaccgg tgggtccagc tcgcgttcat cgctccggtg atgttctaca
     2221 cggggtggcc gatccatcga acgggttggc tcacgcttcg ccaccgcacg gcggacatga
     2281 atgccctcat cacgatcggc acgaccgcgg ccttcaccta cagcctgttc gtgaccgttg
     2341 tgccatctct ggtgccagag gagctccgag aggtctatta cgaggccgtc ggcgtgatcc
     2401 tcacgttgat cctcctcggt cggctgctcg aggcccgcgc gaaggcaggc acgggtgagg
     2461 cgatccgcac gctgatcggt ctgcaggcca agatcgcgag ggtcgtccgc aacggtgagg
     2521 agcgggagat tccgatcggg gaggtcgagg tcggagacga gatcctcgtc cgtcccggcg
     2581 agaagatccc ggtggacggc gaggtgaccg gcgggcactc ggcggtggat gagtcgatgg
     2641 tgaccggcga gccgatcccg gtctccaagg acgtgggcga cgaggtgatc ggcgcgacga
     2701 tcaaccagac cggcgcgttc cggttgcgcg ccaccaaggt cgggcgcgac acgatgctcg
     2761 cccagatcat caagctcgtc gaggccgcac aggcgtcgaa ggcgccgatc cagcgtctcg
     2821 ccgacctggt cgcgagctac ttcgtccccg cggccgtgtt catcgcgatc gggaccttcg
     2881 tcatctggtt cgacttcggc cccgagccgg cgctcacgtt cgccctcgtg agcgcggtct
     2941 cggtgctcat catcgcgtgc ccctgcgcgc tcgggctcgc gacaccgctc tcgatcatgg
     3001 tcggtaccgg caagggcgcg gagaacgggg tcctgatccg gtcggccgag gcgctggaga
     3061 ccgcccacaa gctggacacg atcgttctcg acaagaccgg gacgatcacg aagggcgcgc
     3121 cggccttgac cgatgtcgcc gtcaacgacg gcttcgccga ggaggaactg ctccggctcg
     3181 tggcttccgc agagcgctcc tccgagcatc cgctcgcctc ggcgatcgtg gccggcgcag
     3241 aagtgcggcg gatcgccctc gtcgaaccga gcgagttcga ctcggtcacc ggccagggtg
     3301 tccacgcgac cgtcgagggg cgcagcgtgc tcgtcgggcg gaagcgactg ctcgagtccg
     3361 ccggagtcga cccgtcggcc ctcgagtcga cggcgtcgcg agtcgccgag gaggggaaga
     3421 cggcgatcta cgccgcggtc gacggacgcg ccgcgggcgt cgtggcggtc gcggacacgc
     3481 tcaaggcgga ctcacctgcc gcggttggcg cactccgcga cctcgggctc gaggtcgtca
     3541 tgatcaccgg cgacaacgcc cgcaccgccg aggcggtcgc gcgccaggtc gggatcgagc
     3601 gcgtcctcgc cgaggtgctc ccgcaggaca agtcgctcga ggtgcggcgg ctccaggacg
     3661 agggcaagct ggtggcgatg gtcggcgacg ggatcaacga cgcgccggcg ctcgctcagt
     3721 ccgacatcgg catcgcgatc gggaccggga ccgacgtcgc gatcgaggcc tcggacgtca
     3781 cgctgatctc cggcgagctg cgggggatcg tctcggcgct cgcactcagc cg
//
LOCUS       DATBRK010000436         6058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2126832, whole genome shotgun sequence.
ACCESSION   DATBRK010000436 DATBRK010000000
VERSION     DATBRK010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2126832"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1146)
                     /locus_tag="VIH70_12850"
     CDS             complement(28..1146)
                     /locus_tag="VIH70_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8117298.1"
                     /translation="MNRRTIPGLESPDPLLEREIRGRLDEVEAALEKAVRADSDMLAE
                     TAQHLLAAGGKRFRPMLVLLSGYFGDPSDPRLVPGSVSIELVHQATLYHDDVIDEADE
                     RHAVQSANARWSNTVAILTGDYLFAKASEISTDLGTDICALLARTIATLCDGQIREVE
                     AAGRVEQSEADYLDIIRRKTGALIATSCRLGGMLSDAPAEALDVLEQYGEALGLAFQL
                     SDDIMDLTASQETLGKEPGQDLREGVYTLPVLHALGGPRGGELREVLEDGPPSGERLD
                     RALEIVRAPGHLARARSAVVSEVDRAHALTERLPAGPARTALGQLAEFLATRCDARGS
                     GGSGSERGRASGSPPEQGPGGSRSERGRASGSPPERKS"
     gene            complement(1187..2632)
                     /locus_tag="VIH70_12855"
     CDS             complement(1187..2632)
                     /locus_tag="VIH70_12855"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY8117299.1"
                     /translation="MPTPAVDLGPVLPELILVGVGVVLLLAGVAVRKASPTTLLVLCL
                     GGIAAAAAATVRLWDWDGGPTVLAGSISTDRFGVVVRVIILGVAALGAVLGHHYLERS
                     GENRAEFSPLLLFATAGMTLFAVSADLIMAFLALEILSLSLYVLTGFSTRLGSIEGAM
                     KYFLLGSFSSAFFLFGIAMAYGASGSTRLSAIAGALAGRTGSVALAVTAMGLLAVGFA
                     FKVSAVPFHMWTPDVYQGAPTAVTGFMSAGTKVAAFAAFIRVFNISFQPITWDWQPVV
                     GILAAVSIVAGSLLAIAQTDIKRLLAYSSISHAGFVLLGLTAAGEEGIGAALFYLVAY
                     AATVIGAFGVVMVVSARGERLTSLSAYAGLARRSPVAAALLTLFLLSLAGIPPTVGFV
                     AKVAVFRSATEAGYWFLVLIGVLASVVAAFVYLRVIVLMFMREPEEDTHPDESWLPRV
                     ALAVPAVLVVVLGVFPGLIVGLLEKAAVLRW"
     gene            complement(2632..4221)
                     /locus_tag="VIH70_12860"
     CDS             complement(2632..4221)
                     /locus_tag="VIH70_12860"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY8117300.1"
                     /translation="MPWLTVVTFLPLVGIPVLALWRGASDLHARAVALVVSLATFLVA
                     LGTLGAFDPSVAGYQLVERADWLPSAGVSYLVGVDGFSIWLVVLTAFMTPVAIVSSWG
                     VSHRVRLFMALTLLLETAIVGSFLALDLLLFFVFFEALLVPMFLVIGMWGSSRRVYAS
                     LKFFLYTMAGSAFLLLGIVYLWFQTSQQLVHASFDLRQMERLSLATTTQRWLFLAFFV
                     AFAVKVPIFPLHTWLPDAHTEAPTNGSIVLAALLLKAGPYGLLRFNLDLFPEASRYFA
                     DAIGILAVVGIVYGAIVAMMQTDVKRLVAYSSVSHMGFVILGIFTFTMQGTSGAVMQM
                     VNHGLSTGLLFFVIGALYERTHTRDLSEMGGLATVTPWIAAAFLVATLSSIGLPGLNN
                     FVGEFLVILGSFGADRVLGALAVTGVVLAAVYMLWAYQRAFQGPGAERHRGLADLVPR
                     ELAAVVPVVAVMLVLGVAPNLVLDRINPSSAGVVAWVRSVNVDQPGLPGGLRGKLPPP
                     DDAAVSATAAGGGAGGAGGGP"
     gene            complement(4224..>6058)
                     /gene="nuoL"
                     /locus_tag="VIH70_12865"
     CDS             complement(4224..>6058)
                     /gene="nuoL"
                     /locus_tag="VIH70_12865"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY8117301.1"
                     /translation="GAAASLVLGRRLGKAAGWLATGLVGLGFVLAVATLLDLLGLPAD
                     QRVHVADLFDWISVGTFSVDVALRVDPLSITMTLVVTGVGALIHLYAIGYMDHDPRFG
                     TFFAYLNLFVFFMLMLVLADNYLLLYLGWEGVGLCSYLLIGFWFERPSASSAATKAFV
                     TTRIGDAAMLLGIALIWLHVGSLDFGAVFGRGAGELTEGAATAIALLLLAGAIGKSAQ
                     LPLHVWLPDAMEGPTPVSALIHAATMVTAGVYLVVRSHVLFELSGTALTVVLVVGLVS
                     ALYAALASIGQFDLKRALAYSTMSQIGFMFFAAGMRFYTGAMLLLVCHAFYKALLFLT
                     AGNVLHGLRDETDMRRMGGLRADMPLSAAWFAIGALSLAGIPPLAGFFAKDQIVGFAA
                     TSGRESAWVLASVGAFLSALYIARPLFMTFFGSRRGEEHAHEAPAPMQAPLAVLAFLT
                     VVGGLFLGLTAEGGRLARFLEPVVGAVEHGEAALSDAALIAISIVLALAAVAVAFWIW
                     ASGRVDWQAFPQRQPELAGWLSNAFYLNTLYGWLVQHPGKAFGRGLIVVDDRVIDGAV
                     NEVAEDVVEASRVAPRIQRGFVRSYALAFLLGAVALLLFLGLRA"
BASE COUNT         1101 a         2174 c         2098 g          685 t
ORIGIN      
        1 ttctagcaag cgtcccccgc ctccctctca gctcttccgc tccggggggc taccgctcgc
       61 gcgtccgcgc tcgctccttg agccccccgg cccctgctcg ggggggctac cgctcgcgcg
      121 tccgcgctcg cttcccgagc cccccgaccc ccgcgcgtcg cagcgcgtcg ccaggaactc
      181 ggccagctgc ccgagcgcgg tccgggccgg accggccggg agccgttcgg tcagtgcgtg
      241 ggcgcggtcg acctcggaga ccacggccga acgcgcgcgc gcgaggtgcc ctggggctcg
      301 gacgatctcg agcgcgcggt ccagacgctc cccgctcggg ggtccgtcct ccaggacctc
      361 acgcagttcg cccccccggg gcccccccag cgcgtgcagg accgggagcg tgtagacgcc
      421 ctcgcgcagg tcctgtcccg gctccttgcc gagggtctcc tggctggccg tcagatccat
      481 gatgtcgtcg gagagctgga aggccagacc gagcgcctcg ccgtactgct cgagcacgtc
      541 gagggcctcg gccggagcgt cggacagcat cccgcccagc cggcacgagg tggcgatcag
      601 cgcgccggtc ttccggcgga tgatgtcgag gtaatcggcc tcggactgct cgacccgtcc
      661 ggcggcctcg acctcgcgga tctggccgtc gcacaacgtc gcgatcgtcc gggccagcag
      721 cgcgcagatg tcggtcccga gatcggtcga gatctccgag gccttggcga acaggtagtc
      781 gccggtgagg atcgcgacgg tgttgctcca ccgagcgttc gccgactgca ccgcgtgtcg
      841 ctcgtcggcc tcgtcgatca cgtcgtcgtg gtacagcgtc gcctggtgca cgagctcgat
      901 cgagaccgag cccgggacca gcctcgggtc cgagggatcg ccgaagtagc cggagagcag
      961 gacgagcatc ggccggaacc gtttcccgcc cgccgcgagc aggtgctgcg cggtctcggc
     1021 cagcatgtcc gaatcggctc gcacggcctt ctccagcgca gcctcgacct cgtccaggcg
     1081 tccccggatc tcacgttcga gcaaaggatc gggggactcg aggcccggga tcgtgcggcg
     1141 gttcatcgcc ccgaccctac cgtcatccgc agaccggtgt ggcgcgtcac catcgcagca
     1201 ccgccgcctt ttcgaggagg cccacgatca ggcccgggaa cacgccgagg accacgacga
     1261 ggaccgccgg caccgcgagg gcgacgcgcg gcaaccacga ctcgtccgga tgtgtgtcct
     1321 cctccggctc gcgcatgaac atcagcacga tgacccgcag gtagacgaag gccgccacga
     1381 cgctcgcgag caccccgatc aacaccagga accagtaccc ggcctcggtc gcggaccgga
     1441 agaccgccac cttggccacg aagccgaccg tcggggggat ccccgcgagc gagagcagga
     1501 acagcgtgag cagggcggcc gccacgggac tccgccgggc gagtccggcg taggccgaga
     1561 gcgaggtgag gcgctcgccc cgggccgaga ccaccatgac caccccgaag gccccgatca
     1621 ccgtcgccgc gtacgccacg aggtagaaca aggccgcgcc gatcccctcc tcccccgccg
     1681 ccgtgagtcc gagcaggacg aaccctgcgt ggctgatgct cgagtacgcg aggaggcgct
     1741 tgatgtcggt ctgcgcgatc gcgaggagac tccccgccac gatcgagacc gccgccagga
     1801 tccccacgac gggctgccag tcccaggtga tcggctggaa cgagatgttg aacacgcgga
     1861 taaaggcggc gaacgccgcg accttggtcc cggcggacat gaagccggtc accgcggtcg
     1921 gcgctccctg gtagacgtcg ggcgtccaca tgtggaacgg caccgccgac accttgaacg
     1981 cgaaccccac cgcgagcagc cccatcgcgg tcacggcgag cgcgaccgaa cctgttcggc
     2041 cggccagcgc gccggcgatc gccgagagcc gtgtggaacc cgacgcgccg taggccatcg
     2101 cgatcccgaa caggaagaag gccgaggaga acgagccgag caggaagtac ttcatcgcgc
     2161 cctcgatcga gccgagcctg gtggagaagc cggtcagcac gtacagggac agcgacagga
     2221 tctccagcgc gaggaacgcc atgatcaggt ccgccgagac ggcgaacaac gtcatcccgg
     2281 cggtcgcgaa cagcagcaag ggggagaact cggccctgtt ctcccccgag cgctcgaggt
     2341 agtggtgccc gagcaccgcg cccagcgcgg cgaccccgag gatgatcacg cggacgacga
     2401 ccccgaatcg atccgtcgag atcgacccgg ccaggaccgt gggaccgccg tcccagtccc
     2461 agagccggac ggtcgctgcg gcggccgcgg cgatgccgcc caggcagagc accaacaagg
     2521 tcgtgggcga ggccttccgc acggcgaccc cggcgagcaa caggaccacg ccgacgccga
     2581 ccaggatcag ctcgggcagg accgggccga gatcgacggc cggggtcggc atcagggccc
     2641 acccccggcg cccccggccc cgcccccggc cgcagtcgcc gaaacggcgg cgtcatccgg
     2701 gggcgggagc ttccctcgca gcccgcccgg gagccctggc tggtcgacgt tgacgctccg
     2761 cacccaggcc accacgccgg ccgaggacgg gttgatccgg tccaggacga ggttcggagc
     2821 gacgccgagc accagcatca ccgcgacgac ggggacgacc gccgcgagct cgcgcggcac
     2881 gaggtcggcc agcccccggt ggcgttcggc ccccggaccc tggaacgccc gctggtaggc
     2941 ccagagcatg tagaccgcgg cgagcacgac gccggtgacg gccagggcgc ccaggacgcg
     3001 atcggcgccg aaggagccca ggatcacgag gaactcaccg acgaagttgt tcaggccggg
     3061 gagcccgatc gaggagaggg tcgccacgag gaaggccgcc gcgatccagg gcgtcaccgt
     3121 cgccagccca cccatctccg acaggtcgcg cgtgtgggtg cgctcgtaca gcgcgccgat
     3181 cacgaagaac agcaggccgg tcgagagccc gtggttcacc atctgcatca cggcgccgct
     3241 cgtgccctgc atcgtgaacg tgaagatgcc gaggatcacg aaccccatgt ggctgaccga
     3301 tgagtacgcg accaggcgtt tcacgtcggt ctgcatcatc gcgacgatcg ccccgtagac
     3361 gatcccgacg accgccagga tgccgatcgc gtcggcgaag tagcgggacg cctcggggaa
     3421 caggtcgagg ttgaaccgga gcaggccgta cggtccggcc ttcaggagca acgcggccag
     3481 gacgatcgag ccgttcgtcg gcgcctcggt gtgcgcgtcg gggagccacg tgtgcagcgg
     3541 gaagatcggc accttgaccg cgaacgcgac gaagaaggcc aggaacaacc accgctgcgt
     3601 cgtcgtggcg agcgagagcc gctccatctg acggaggtcg aagctcgcgt ggacgagctg
     3661 ctgggaggtc tggaaccaca ggtagacgat cccgagcaac aggaaggccg agcccgccat
     3721 cgtgtacagg aagaacttca ggctcgcgta cacgcgccgg gacgagcccc acatcccgat
     3781 cacgaggaac atcgggacga gcagcgcctc gaagaagacg aagaacagca gcaggtcgag
     3841 cgcgaggaac gaacccacga tcgcggtctc gagcaacagc gtgagcgcca tgaacaaccg
     3901 gacccgatgg ctcacgcccc aggacgagac gatcgccacc ggcgtcatga acgcggtgag
     3961 cacgaccagc cagatgctga acccgtcgac gcccacgagg taggacacgc ccgccgacgg
     4021 cagccagtcc gcgcgctcga ccagctggta gccggcgacc gagggatcga acgcgcccag
     4081 cgtgccgagc gcgaccagga acgtggccag cgagaccacc agggcgacgg cacgggcgtg
     4141 gaggtcgctc gccccgcgcc acagcgcgag gacggggatc cccacgagcg gcaggaaggt
     4201 gacgacggtc agccagggca tcgtcaggcc ctcagtccca ggaacaacag gagcgccacc
     4261 gcgccgagca ggaacgcgag cgcgtacgag cgcacgaacc cccgctggat cctcggggcg
     4321 acccgcgacg cctcgaccac gtcctcggct acctcgttca ccgctccgtc gatcacgcgg
     4381 tcgtcgacca cgatgaggcc gcgaccgaac gccttgccgg ggtgctgcac cagccagccg
     4441 tagagcgtgt tgaggtagaa ggcgttcgag agccacccgg cgagctcggg ctggcgttgg
     4501 gggaaggcct gccagtcgac gcggccggag gcccagatcc agaaggccac ggcgaccgcc
     4561 gcgagcgcga gcacgatcga gatcgcgatc agggcggcgt cggacagggc ggcctctccg
     4621 tgttcgaccg cgcccacaac cggttcgagg aagcgggcca gccggccgcc ctcggcggtc
     4681 aggcccaaga acagcccgcc gacgaccgtg aggaacgcga ggacggcgag cggcgcctgc
     4741 atcggcgccg gggcctcgtg tgcgtgctcc tcgccgcgcc ggcttccgaa gaacgtcatg
     4801 aacagcggtc gcgcgatgta cagcgccgac aggaaggcgc cgaccgacgc caggacccag
     4861 gccgactcgc ggccgctcgt ggctgcgaac ccgacgatct ggtccttcgc gaagaagccc
     4921 gcgagcggcg ggatgcccgc gagcgacaac gcgccgatcg cgaaccaggc ggccgagagc
     4981 ggcatgtccg cccgcaggcc ccccatccgc cgcatgtcgg tctcgtcgcg caaaccgtgc
     5041 aggacgttgc cggccgtgag gaagagcagg gccttgtaga acgcatggca gacgagcagc
     5101 agcatggctc cggtgtagaa gcgcatgccg gccgcgaaga acatgaaccc gatctggctc
     5161 atcgtcgagt acgcgagcgc gcgcttcagg tcgaactggc cgatcgacgc gagcgcggcg
     5221 tacaacgccg agaccaggcc gaccaccagg acgaccgtga gcgccgtccc ggagagctcg
     5281 aacaggacgt gcgagcgcac gacgaggtag acgccggccg tcaccatcgt cgccgcgtgg
     5341 atcagcgccg acaccggcgt gggaccctcc atcgcgtcgg ggagccagac gtgcagcggc
     5401 agctgcgccg acttgccgat cgcacccgcg agaaggagca gggcgatcgc ggtggccgcg
     5461 ccctccgtca gctcgccggc tccccggccg aacacggcgc cgaagtccag cgacccgacg
     5521 tgcagccaga tcagcgcgat cccgagcagc atcgcggcgt cgccgatccg cgtcgtgacg
     5581 aaggccttcg tcgcggcgct cgaggccgat ggacgctcga accagaaccc gatcagcagg
     5641 tacgagcaca ggccgacgcc ctcccacccc aggtacaaca ggaggtagtt gtccgcgagg
     5701 acgagcatca gcatgaagaa gacgaacagg ttcaggtagg cgaagaacgt tccgaaccgc
     5761 gggtcgtggt ccatgtagcc gatcgcgtac aggtggatca gcgctccgac gcccgtgacc
     5821 accagcgtca tcgtgatcga gagggggtcg acccgcaggg cgacgtcgac cgagaacgtc
     5881 ccgaccgaga tccagtcgaa caggtccgcc acgtgcacgc gttggtccgc ggggagcccg
     5941 agcaggtcga ggagcgtcgc caccgcgagc acgaagccga gcccgacgag ccccgtcgcg
     6001 agccaccccg cggccttgcc gagccgccgt ccgagcacga ggctcgccgc ggcgcccg
//
LOCUS       DATBRK010000437         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1264494, whole genome shotgun sequence.
ACCESSION   DATBRK010000437 DATBRK010000000
VERSION     DATBRK010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264494"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            527..751
                     /locus_tag="VIH70_12870"
     CDS             527..751
                     /locus_tag="VIH70_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117302.1"
                     /translation="MVLKAGEPVAFRSAPLPRSYQIAWEDEEHVLVLAREADRGRVVY
                     RCDVTRERCARVWTFEAASDRYAAWLVPKA"
     gene            complement(996..1430)
                     /locus_tag="VIH70_12875"
                     /pseudo
     CDS             complement(996..1430)
                     /locus_tag="VIH70_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            1620..>1802
                     /locus_tag="VIH70_12880"
     CDS             1620..>1802
                     /locus_tag="VIH70_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006346660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117303.1"
                     /translation="MAQLDTQILPLLPLTSGVVLPGMVVTLTIESDEARRALAASESS
                     GGELLLVPRIGSSYAKI"
BASE COUNT          275 a          613 c          620 g          294 t
ORIGIN      
        1 cccggtcggc gacctgcttg aagagagccc gcgccccctc ggcctcggtc atcgagcaag
       61 gataggccca ggtgaggggg acggggttgc ggtttcctgc ccaggaggaa ccgagcccgt
      121 ctcggcgagc gtcgaggcgc gcatcgcctg acgatggaga gaggggctcc gcgcctgacc
      181 gcgctcgccc tcgcgggact tctcgccttt gcggcgtgcg gggaccgcga cgatgccatc
      241 gggcgcgatg tcacttcccc gacgggttcg acagcttcca cgggccccac cagtccacag
      301 accgcgtcta tcgaggccac gatcgaggtc ggcgcggacc cgataggcat cgcgtcgagc
      361 cagggatcgc tctgggtggt cgacgcggac atcgacgcgg agcgagggtc cgtctcgagg
      421 atcgacccgc gaggagcacg ccctcctgtc gccgtccggg cggtggatcg cggcgatcga
      481 ggggcatgcc gacagtggtt tcgacgggct cgagcagaac cgtctcgtgg tgctcaaagc
      541 cggcgagccg gtcgcgttcc gaagcgcgcc gctgccacgc agctatcaga tcgcctggga
      601 ggacgaggag catgtgctgg tcctggcgcg agaggccgac cggggccggg tcgtctaccg
      661 atgtgacgtg accagggaac gttgcgcgcg cgtctggacg ttcgaagccg cctccgatcg
      721 gtacgccgcg tggctcgtgc caaaggcgtg agccctcacg ccaccgaccc gagtgcgaaa
      781 ccgcatcgtc tgcagtacgc aaccctcacc gcgatgggtt cttcgtccgt cgtctcgagg
      841 accttcacgg cgagggtctc gtcaagggcg gcgaagcagt tcgggcacaa cggcgcatcc
      901 tcggcagcga gtacgggggt gggggacatc tctgtggcga gctcctcctc cctctcggcg
      961 ccgcccaccg ccttgcgaag ctccgcgccg gtcgcgtagc cggataggag ctggatcacg
     1021 gtctggcgga cgcggttgag gtcggcgccg agcttcacca ggacctgcgc ggcgacgccc
     1081 tcgccctctc ggatcaggcc gagcaggatg tgctcggtcc cgatgtagtt gtgcccgagc
     1141 tgcagggcct cccgcagcga gagctccagg accttcttgg cccgcggcgt gaacgggatg
     1201 tgacccgtga tcgccgcctg gccctggccg atgacctctt cgacctcccg acgcaccgcg
     1261 tcgagcctga tgttcaggct gtcgagcgcc ttggcggcga cgccgtctcg ctcgcgcacg
     1321 aggccgagca ggatgtgctc ggtcccgatg tagttgtggc tcagcagcct cgcctcttcc
     1381 tgggcgagga ccacgacgcg acgggcccgg tcggtgaagc gctcgaacac ctgcaccgag
     1441 gataggcctc gatctccttg ccggcggccg atatcgatct cggtatcgat accgtcgacg
     1501 agatcgcgat tcatctcggc cggatcgcct tgagactgta atcctgctta caaccgggga
     1561 ataccgtgcc tgagcagcgc gttgactcgg ccgagacgac tcttggagga gaaaccgaca
     1621 tggcacagct cgacacccag atcctgccgc tcctgcctct gacctcgggc gtggtcctgc
     1681 cgggcatggt cgtgaccctc acgatcgagt ccgacgaggc ccgccgcgcg ctcgcagcat
     1741 ccgagtcctc gggcggcgag ctcctgctcg tgccccggat cggctcgagc tacgcgaaga
     1801 tc
//
LOCUS       DATBRK010000438         4595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_540105, whole genome shotgun sequence.
ACCESSION   DATBRK010000438 DATBRK010000000
VERSION     DATBRK010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540105"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..1498)
                     /locus_tag="VIH70_12885"
     CDS             complement(338..1498)
                     /locus_tag="VIH70_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8117304.1"
                     /translation="MPEHEERPRGNGSDHPGEMSSPRAIQARASRVRRIVAGFTAVLV
                     LASVPAVGLSRFASADENVFEQTKQELKHSKHELQKTKDRARAQTRKIRALQRVMNRL
                     ATRISRAEQEIQRIEIRLDKLERQTARLQVRAALLQAKLDERSREAYMSGGVPVLYVL
                     TATSAADAASRMSFLTEMNRRDAVLAAKVRETTDRIAQIEAEVVRARQIVELKKRSLE
                     LDREELQRKMTQSRRLVAKLHGRIEEIQIEISLIRPLAVCPVGGPHAVSDNFGDIRHE
                     PGGGTHIHQGDDIMAATGTPILAPFDGVASVSHSFLGGLGVYVHGEYGYVYNAHLSLL
                     GTMGPVETGDVIGYVGSTGSSSAPHDHFEWHPENGPAVDPYDYLMLVCSVPL"
     gene            1662..3224
                     /locus_tag="VIH70_12890"
     CDS             1662..3224
                     /locus_tag="VIH70_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117305.1"
                     /translation="MTDQITGVLRRYGRFLWGGAALLLLLVLLGSVVLSGSSVGGAEA
                     DAQARAERLSGSVIEQVLKPDLLVRDITGVAARDLTVLVQAVTLSDERITTVRIWRLD
                     GALIFSSAERDDASVVAADDRWIGQALDGRTVSALSSAGTYHDGLQRPDEELFQTFVP
                     ITLSDDGTVDGVVQIDQRYSPIHDQGYGNRRALQLVVFLLLIGVGVMFVRSQRGAHAS
                     LEDGPPERRQGHGRRAEDLDVLARAERAERAARETEGRVRELEAALAAAPTSAALEEL
                     DLKLRASEAEREELTATIQRLRADLSERDTDVALARDGSGSRADAKRVNQLIADAKSR
                     VAAAERKAAFAEEKTQEAAKRATAAADRALEMEAQLKEAEQRAEDARKLAAAVDTRGS
                     GAVKRFGEAERRALEARQRANDAADRATQAERQARELSGTAAEAERRAAASERRALAA
                     EQRVADVERRAAETAQLVTPSEDRASADRVASATSPTGKSSRRGSGTSRAPVAQTSSS
                     SGKRKTPSRTPG"
     gene            complement(3268..3849)
                     /locus_tag="VIH70_12895"
     CDS             complement(3268..3849)
                     /locus_tag="VIH70_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117306.1"
                     /translation="MPTELETPVATLAEPIHAWRTWTLVGSRDGSRVRLAPIAGDGRP
                     WPPRRPAEASCTRNRSHVRPELECTCGLHAVETPDELRRTRDPAVLGTVALWGRIVEH
                     EHGFRAALAYPQRLRLLCYLCFTLWGRNGPGDCEVVVRHRGGRMVPLCGPHLELSRRY
                     GYRVPRILSARAIESELLATYAVDPLRELARAN"
     gene            complement(3853..3990)
                     /locus_tag="VIH70_12900"
     CDS             complement(3853..3990)
                     /locus_tag="VIH70_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117307.1"
                     /translation="MDIGRPKRIIEVDPVSLPLPEVVPDLEPATPEPAPVEPDRSRPA
                     P"
     gene            complement(4062..>4595)
                     /locus_tag="VIH70_12905"
     CDS             complement(4062..>4595)
                     /locus_tag="VIH70_12905"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8117308.1"
                     /translation="IIRIEPDGDVPSDNPFGDRVWVFGIRNSFGFAFDPQTDSLWETE
                     NGPACNDEINRIRGGENYGWGPSETCAGASPGNTNQDGPNPIMPELFYQSPIGVTGIA
                     FCDGCHLGRRNDDAAFFGAVNNGQVTRIVFNAQRTAIAGHTVVLDRGGSTLSYEIGPG
                     GRIFFSDFDGINKLVRR"
BASE COUNT          687 a         1573 c         1659 g          676 t
ORIGIN      
        1 gagagacagg accggtgcga tcgtgcgcgc tcgaccccag gcggcggttc tccgcctccg
       61 tcctgtgccg ccccgggtcc tcacgtcgca tccttcctcg acctcgcccg ggtgttccca
      121 tccatcggtc gcccggcgtc ctcgcgtagt ctcggctcaa cgacccaccc ccgcatcgac
      181 ctttcgggtc catattgcgg tagggaggtg agggggaacc ccgttggggc gggggaggcc
      241 gaaaggcgta gcgaaagcgt gaaggcacta ggacaaattt cctgcgtacg tcttgcgagc
      301 cgcgggctcg ccgagcggcg ccgcgccgcg aggaacctca gagcgggacg ctgcagacga
      361 gcatgaggta gtcgtagggg tccaccgccg gtccgttctc cggatgccac tcgaagtggt
      421 cgtgtggggc gctcgaggag ccggtcgacc cgacatatcc gatgacgtcg ccggtctcga
      481 ccgggcccat ggtgccgagc agcgacagat gcgcgttgta cacgtagcca tattcgccgt
      541 ggacgtagac gcccagcccg cccaggaagc tgtgggagac ggacgcgacg ccgtcgaacg
      601 gggcgaggat cggtgtcccg gttgccgcca tgatgtcgtc gccttggtgg atatgggtcc
      661 cacccccggg ctcgtggcgt atgtcgccga agttgtcgga gaccgcgtgc gggcccccca
      721 cggggcacac ggccaacggc cggatgagcg agatctcgat ctggatctcc tcgatcctcc
      781 cgtggagctt ggcgacgagg cgtctggact gcgtcatctt gcgctggagc tcctcgcggt
      841 ccagctccag gcttcgcttc ttgagttcca cgatctggcg ggcccgcacg acctcggcct
      901 cgatctgcgc gatacgatcg gtcgtctccc gcaccttggc ggcgagcacg gcgtcgcgac
      961 ggttcatctc ggtgaggaac gacatccgcg acgcggcatc cgccgcggac gtcgccgtca
     1021 gcacgtagag gaccgggacg ccgccggaca tgtaggcctc gcggctgcgc tcgtcgagct
     1081 tggcctgcag cagcgcggcg cggacctgca gcctcgccgt ctgccgctcg agcttgtcga
     1141 ggcggatctc gatccgctgg atctcctgct cggcccgaga gatgcgcgtg gccaaacggt
     1201 tcatcaccct ctgcagagcg cggatcttcc gcgtctgtgc gcgggcccgg tccttggtct
     1261 tctgcaattc gtgcttgctg tgcttcagct cctgcttggt ctgctcgaac acgttctcgt
     1321 cggccgaggc gaacctcgaa agaccgaccg cgggaaccga tgccaacacg agcaccgccg
     1381 tgaatccggc cacgatccgg cgcacgcgcg atgcgcgtgc ctggatggcg cgcggactcg
     1441 acatctcgcc ggggtgatcg gagccgttcc cccggggacg ttcttcgtgt tcgggcattc
     1501 agcgggcatc ggcagaccaa gacgatgcct taacacgatg aacggtgtcg ccgccggaac
     1561 cgcgcgaaac ctctcgcccg ggttcaagcc tgagcgccgc ggggccgaga gagaagggtg
     1621 ggggtgctcg acgcgtgtgc gggcagccgt cgggaaggta catgaccgat cagataacgg
     1681 gagtcctccg ccgctacggc cgcttcctct gggggggagc cgccctgctg ctcctgctcg
     1741 tcctgctggg gtccgtcgtc ctgtccggtt cctcggtcgg cggggccgag gcggacgccc
     1801 aggctcgggc cgagcgcctc tcgggctcgg tcatcgagca ggtgctgaag cccgacctgc
     1861 tcgtacggga catcaccggt gtcgccgctc gcgacctgac ggtcctggtg caggcggtga
     1921 ctctgtcgga cgagcggatc acgaccgtgc ggatctggcg gctggacggg gcgctgatct
     1981 tctcgagcgc cgagcgggac gacgcgagcg tcgtcgccgc cgacgatcgg tggatcgggc
     2041 aggcgctcga cggccggacc gtgagcgctc tctcgagcgc cggcacatac cacgacggtc
     2101 tccagcgacc ggatgaggag ctgttccaga ccttcgtccc catcacgctg tcggacgacg
     2161 gcacggtcga cggtgtcgtc cagatcgacc aacgctactc gccgatccac gaccaggggt
     2221 acggcaaccg gcgtgcgctg cagctcgtcg tgttcctgct gctcatcggc gtcggcgtga
     2281 tgttcgtccg gtcgcagcgg ggggctcatg ccagcctgga ggatggacca cccgaacgcc
     2341 ggcaggggca cggtcgccgc gccgaggatc tggacgtcct ggctcgagcc gagagggccg
     2401 aacgcgcggc gcgcgagacc gaggggcgtg tgcgcgagct ggaggcggcc ctcgcggcag
     2461 cgccgaccag cgcggcgctc gaggagctcg acctcaagct ccgcgcctcg gaagccgagc
     2521 gcgaggagct gacggccacc atccagcgcc ttcgggccga cctgtcggag cgtgacacag
     2581 acgtcgcgct cgcgcgcgac ggctccggct cgcgggccga cgcgaagcgc gtcaaccagc
     2641 tgatcgccga cgcgaagagc agggtcgccg cggccgaacg caaggccgcc ttcgccgagg
     2701 agaagaccca ggaggcggcg aagcgggcaa ccgccgccgc cgaccgggcg ctcgagatgg
     2761 aggcccagct caaggaggcc gaacagaggg cggaagacgc ccggaagctc gcggccgcgg
     2821 tggacacgcg gggatccggg gccgtgaaga ggttcgggga ggccgaacgc cgtgccctgg
     2881 aggcgaggca gagggcgaat gacgcggcgg accgtgccac ccaggcggag aggcaggcgc
     2941 gcgagctctc gggaacggcg gccgaggccg agaggcgagc ggccgcaagc gagcggcgtg
     3001 ccctggcggc cgagcagagg gtcgccgacg tcgagcggag ggccgccgag acggcgcagc
     3061 tcgtcacgcc ttcagaggac cgagcgtccg ccgatcgcgt ggcgtcggcc acgtctccga
     3121 cgggcaagag ctcgaggcga ggatcgggaa cctcgagggc gcccgtcgcg cagacgtcgt
     3181 cgagctccgg caagcgcaag actccttcgc gaacgcctgg gtagcgccgg tcgtccctcg
     3241 catcgtcagg ccgagatcga ctcggcccta gttcgcgcgg gcgagctccc gaagcggatc
     3301 gaccgcgtag gtcgccagca actccgactc gatcgcccga gcggacagga tccgcgggac
     3361 gcgatacccg taccggcgac tgagctcgag gtgcggcccg cacagcggca ccatccttcc
     3421 cccgcggtgc cgcacgacca cctcgcagtc gccgggcccg ttccgccccc acaacgtgaa
     3481 acagaggtag cagagcagcc ggagccgctg cgggtaggcg agcgccgccc ggaacccgtg
     3541 ctcgtgttcg acgatccgac cccacagcgc gacggtccca agcaccgccg ggtcgcgggt
     3601 cctccgcagt tcgtcggggg tctcgacggc atgcaggccg cacgtgcact ccaactcggg
     3661 ccgaacgtgc gatcggttcc gggtgcacga ggcctcggcc gggcgccgcg gcggccaggg
     3721 cctgccgtcg ccggcgatcg gcgcgagccg cacccgcgac ccgtctcgcg aacccacgag
     3781 cgtccacgtc cgccaggcgt ggatcggttc ggcgagggtc gcgaccgggg tctcgagctc
     3841 ggtgggcatc ggctacggag ccggccggga gcggtcgggc tccacgggcg cgggttcggg
     3901 cgtcgccggc tcgaggtcgg gaaccacctc gggcagcggg agcgacacgg gatcgacctc
     3961 gatgatccgc ttcggccgac cgatgtccat tggcgggctc ctcgggtcgt tcctgatgcc
     4021 gagcgtacct ccgcgcatcg cggccggcca gccccgttcg atcagcggcg gaccagcttg
     4081 ttgatcccgt cgaagtcgct gaagaagatc cgtcccccgg gaccgatctc gtaggagagg
     4141 gtgctcccgc cgcggtcgag gacgacggta tgaccggcga tcgcggtccg ttgagcgttg
     4201 aacacgatcc gggtcacctg gccgttgttc accgcgccga agaacgccgc gtcgtcgttc
     4261 cgccggccga ggtggcagcc gtcgcagaac gcgatgccgg tgacgccgat cgggctctgg
     4321 tagaagagct cgggcatgat cgggttcggt ccgtcctggt tcgtgttgcc cggagaagcg
     4381 ccggcgcagg tctcgctcgg cccccagccg tagttctcgc cgccccggat ccggttgatc
     4441 tcgtcgttgc aggccggccc gttctcggtc tcccacaggc tgtcggtctg cggatcgaac
     4501 gcgaagccga acgagttccg gatcccgaac acccacacgc ggtcgccgaa cgggttgtcg
     4561 ctggggacgt cgccgtcggg ctcgatgcgg atgat
//
LOCUS       DATBRK010000439         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1409801, whole genome shotgun sequence.
ACCESSION   DATBRK010000439 DATBRK010000000
VERSION     DATBRK010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409801"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VIH70_12910"
     CDS             <1..895
                     /locus_tag="VIH70_12910"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY8117309.1"
                     /translation="FSHGFQLIVFAAVGLGFAVKVPMWPFHTWLPDAHTEAPTIGSVL
                     LAGVMLKMGGYGFIRIALPILPYGAQKYAPWIGLLAAIAIVYAALACLAQKDLKRLIA
                     FSSVGHMGFVMLGIATLTSIGINAAIFGMVAHGLITGMLFFCVGSLYDRYHTREIAEI
                     GGGVMQKLPYLGGVFAFVAIASLGLPGLAGFWGEVMALLSSYSPSQSLSTPLFRAFMI
                     AGGVGTILTAGYFLWMLQRVNMGTLPEKWKAEAFGDVAAVEWVSWVPLLVGIVALGLF
                     PGLLTDVTQAPVNSLLGLFGV"
     gene            917..>1906
                     /locus_tag="VIH70_12915"
     CDS             917..>1906
                     /locus_tag="VIH70_12915"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY8117310.1"
                     /translation="MNQAIDYTAILPELILAGTIVLVLVVDAFLAPARKWMAMPLAFA
                     GVLASLIATLTLIGEDRSTFGGSFVVDEFALLFKVFFLSVALLVLMVSFRYVREGRFY
                     QGEFYFLVLTSFLGCVLMPSSRDLLMLFISLELVSAPGFVIAAFRKSDPRSNEAGIKF
                     FLFGVLSTAVMLYGMSLIYGLTGSLNLTEIGHALATPLPQGRETLALASILFIVAGFA
                     FKVSAVPFQFWAPDTYEGAPVPVAAFLATASKAAGFAGLLQLMFVAFPAQYEFWTPIF
                     AVLSILTMTIGNFVALQQRQVVRLLAYSSIAQAGYMLLPFALVSANPVINQDAF"
BASE COUNT          235 a          635 c          642 g          394 t
ORIGIN      
        1 tttctctcac ggcttccagc tgatcgtgtt cgcggcggtc gggctcgggt tcgccgtgaa
       61 ggtgccgatg tggccgttcc acacgtggtt gcccgacgcg cataccgagg cgccgacgat
      121 cggctcggtc ctgctggccg gggtcatgct gaagatgggc gggtacgggt tcatccgcat
      181 cgcgctgccg atcctcccgt acggcgcgca gaagtacgca ccctggatcg ggctgctggc
      241 cgcgatcgcg atcgtctacg ccgcgctcgc gtgcttggcg cagaaggacc tcaagcggct
      301 catcgcgttc agctcggtgg gccacatggg gttcgtgatg ctggggatcg ccacgctcac
      361 ctcgatcggg atcaacgcgg cgatcttcgg gatggtcgcg cacggcctca tcaccgggat
      421 gctcttcttc tgcgtggggt cgctctacga ccgctaccac acgcgcgaga tcgccgagat
      481 cggcggcggc gtgatgcaga agctccccta cctcggaggc gtgttcgcgt tcgtcgcgat
      541 cgcctcgctc ggcctgcccg ggctcgctgg gttctggggc gaggtgatgg cactgctgtc
      601 gagctacagc ccgtcgcagt cgctgtccac gccgctgttc cgagccttca tgatcgccgg
      661 tggcgtcggg acgatcctga ccgcggggta cttcctgtgg atgctgcagc gcgtgaacat
      721 ggggacgctc ccggagaagt ggaaggccga ggcgttcggg gacgtcgccg cggtcgaatg
      781 ggtctcctgg gtcccgctcc tggtcgggat cgtcgcgctg gggctgttcc caggcctgct
      841 caccgacgtc acgcaggcgc cggtcaactc cctgctcggc ttgttcgggg tgtagagggc
      901 gagggccggc gcggcgatga accaggcgat cgactacacg gcgatcctcc cggagctgat
      961 cctggccggg acgatcgtcc tcgtgctggt ggtcgacgcg ttcctcgcgc ctgcccgcaa
     1021 gtggatggcg atgcccctcg ccttcgccgg ggtgctggcg tcgctgatcg ccacgctcac
     1081 gctgatcggc gaggatcgct cgaccttcgg cggctcgttc gtcgtcgacg agttcgcgct
     1141 gctgttcaag gtgttcttcc tgtcggtcgc attgctcgtg ctgatggtgt ccttccgcta
     1201 tgtccgcgag gggcgtttct accagggcga gttctacttc ctggtgctca cctccttcct
     1261 cggctgcgtg ctgatgccgt cgtcgaggga cctgttgatg ctgttcatct cgctcgagct
     1321 cgtgtcggcg ccggggttcg tgatcgcggc gttccgcaag tcggatccgc gatccaacga
     1381 ggcaggcatc aagttcttcc tgttcggcgt cctctcgacc gcggtgatgc tctacgggat
     1441 gagcctgatc tacgggctga ccggcagcct gaatctgacc gagatcggac acgcgctcgc
     1501 cacgccgctg ccgcaagggc gggagacgct ggcgctcgcg tcgatcctgt tcatcgtggc
     1561 cgggttcgcc ttcaaggtga gcgcggtccc gttccagttc tgggcgcccg acacgtacga
     1621 gggcgccccc gtaccggtcg cggcgttcct cgcgaccgcg tccaaggcgg ccggcttcgc
     1681 cgggctcctc cagctgatgt tcgtcgcctt ccccgcccag tacgagttct ggacgccgat
     1741 cttcgcggtg ctgtcgatcc tcacaatgac gatcgggaac ttcgtcgccc tgcagcagcg
     1801 ccaggtcgtg cgcctgctcg cgtactcgag catcgcccag gccggctaca tgctcctgcc
     1861 gttcgccctg gtctcggcga acccggtcat caaccaggac gcgttc
//
LOCUS       DATBRK010000440         5611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_115366, whole genome shotgun sequence.
ACCESSION   DATBRK010000440 DATBRK010000000
VERSION     DATBRK010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115366"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VIH70_12920"
     CDS             complement(<1..1181)
                     /locus_tag="VIH70_12920"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8117311.1"
                     /translation="MTTTFADLGVSGRVTEALGRVGVSDPFAIQSLVLRDAIAGRDVL
                     ARSATGSGKTLAFAIPIVERVRPNDPTPTALVLVPTRELAQQVADDFAEIARATGLRV
                     GVAYGGVSMQQQARGNAKAHVLIATPGRLQDLAERGSVRLDRVRILVLDEADRMLDMG
                     FQPQVDRLVRRIPKDRQTMFFSATLDGAVGHLARAYTKDAVLHEVESAHDTVDGLDHR
                     FVQVESRGKVEALIELLEHDGELTLVFVRTKRGADRLVHKLKQKGLQAEALHGDMPQR
                     ARQRALERFETGKARVLVATDVAARGLDLERISHVVNYDPPQDDKGYVHRVGRTARAG
                     RTGIGITLVTADQQGDVGRMAARLDLHDAFEEEGMKVAPPQVVFSASASGRRSGLRAR
                     RR"
     gene            complement(1388..2038)
                     /locus_tag="VIH70_12925"
     CDS             complement(1388..2038)
                     /locus_tag="VIH70_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY8117312.1"
                     /translation="MATVLASPKPRFRGRIHQVAFFVSLPAGVVLILLANGPAAIAVA
                     SVYAVSLAAVFGSSAVYHRGRWTERARRWLKRLDHSMIYVLIAASYTPVAALVLGGPW
                     EVVLLSVIWTGAVAGVTLKMARPDGLSVVSAALYIVLGWLALVALPQLAREMTRAELV
                     LLLAGGLLYTAGAIMFAMRRPDPSPSIFGYHEVWHSFMVAAAACHYAMILLLLLPH"
     gene            2081..3796
                     /gene="ade"
                     /locus_tag="VIH70_12930"
     CDS             2081..3796
                     /gene="ade"
                     /locus_tag="VIH70_12930"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEY8117313.1"
                     /translation="MTNDLVRRIAVARGDEPADLVLTGGRVLSVFTGEFLEADVAIVG
                     EHVAGVGPGYEGTERLDVSDLILLPGFIDGHMHLETTKLRLDQFARAALPWGTTTVVL
                     DPHEIANVFGIDGVRALLHDADEVPLDAYVMVSSCVPASPFESSGATLTPEDIAELLR
                     TEPRAIGLAEMMDYPAVVAGVEDAVAKVAAAGDRHIDGHAPGLSGRSLNAYLAAGVRS
                     DHECTTYEEALEKRRLGMWIMIRQGSAARNLEALLPLVLEHGITNCLLCTDDREPHDL
                     QEHGHINDVVREAVALGCPPADAVAMATLNAARYHRLHEHGAVAPGYLADIVAVRDLV
                     SFRPERVWKRGRLVADGGRPSPIASSSLPGWMRESMHVRELEPVDFSVVADGAVRVIG
                     VDAGTIVTRALVGEPLRRDGKACADPGRDLAKLAVIERHRDTGRIGVGFAQGFGLQAG
                     ALASSHAHDAHNVVVVGMTDEEMAAAANHLRAIGGGQVVVMGDRVIADLPCPIGGLLS
                     DLTFEEVADRARVLEEAAGELGTTLPSPFMAMSFLALSVIPELKLTDRGLVDVDRFEL
                     VPLQV"
     gene            complement(3806..>5611)
                     /locus_tag="VIH70_12935"
     CDS             complement(3806..>5611)
                     /locus_tag="VIH70_12935"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY8117314.1"
                     /translation="LARQLRADSIRCSTAAGSGHPTSSLSAADLMAVLLAGHFRYDFE
                     RPDALANDHLIFSKGHASPLVYAMFRAAGAIDDAELLTFRKFGSRLEGHPTPVLPWVD
                     VATGSLGQGLPIGVGVALAGKRLLAAPFHVWVVVGDSETAEGSIWEAFDLAGHEGLEN
                     VTAILDINRLGQRGPTELEWDTGAYAARVKAFGWDAIVIDGHDLAEIDDALDRARSAS
                     RPTAIVARTVKGKGVSFLEDKPGWHGKALDAEQAKQALAEIGAEPSRAFEVPSPPAWR
                     PAPLAPSKTPELKRYSEAVATRKAYGEALAALGAARADVVVLDAEVSNSTHAEDFKKT
                     AVDRFFEMYIAEQRMVAVAVGMQVVGFVPFASTFAAFLTRAYDFIRMAAISNVNIKMA
                     GSHAGVSIGEDGPSQMALEDLAMMRAVHGSTVLYPCDGNSTARLVGAMADLPGISFIR
                     TTREKTPVIYEGDEAFPVGGSKTLRSSDADRVTLVGAGITVHEALEAAGSLEADGVAA
                     RVIDCYTIKPIDAETLRAAASQTGLLVTIEDHWIEGGLGDAVLAALADGGELSGTVVK
                     VAVTQMPGSGTPEELRDWAGISASAIADRVRSLLR"
BASE COUNT          832 a         2001 c         1967 g          811 t
ORIGIN      
        1 cgtcggcgcg ctcgcaggcc cgaacgcctc cccgaggcgc tcgcggagaa gaccacctgc
       61 gggggcgcga ccttcatgcc ctcctcctcg aacgcgtcgt gcaggtccag ccgcgcggcc
      121 atccggccga cgtcaccctg ctgatccgcc gtcaccagcg tgatgccgat accggtccgt
      181 ccggcccggg ccgtgcgtcc gacgcggtgg acgtagccct tgtcgtcctg gggcgggtcg
      241 tagttcacga cgtggctgat ccgctccaga tccagaccgc gggcggcgac gtccgtcgcg
      301 accaggaccc tggccttgcc cgtctcgaac cgctcgagcg cacgctgacg cgcccgctgt
      361 ggcatgtcgc cgtggagcgc ctcggcctgc aaacccttct gcttcagctt gtgcaccaga
      421 cggtcggcgc cgcgcttggt ccgcacgaag acgagcgtca gctccccgtc gtgctcgagg
      481 agctcgatca gcgcctcgac cttgccgcgc gactcgacct ggacgaatcg atggtcgagc
      541 ccgtcgacgg tgtcgtgcgc agattcgacc tcgtgcagaa ccgcgtcctt cgtgtaggcg
      601 cgcgccaggt gcccgaccgc gccgtccaac gtggcggaga agaacatcgt ctgccggtcc
      661 ttggggatgc ggcggaccag gcggtcgacc tggggctgga agcccatgtc gagcatccgg
      721 tcggcctcgt cgaggacgag gatccggacg cggtcgagac ggacgctgcc gcgctcggcg
      781 agatcctgca ggcggcccgg ggtggcgatc agcacgtggg cctttgcgtt gccgcgagcc
      841 tgctgttgca tgctgacgcc gccgtaggcg accccgacgc gcaggcccgt ggccctcgcg
      901 atctcggcga aatcgtcggc cacctgctgc gcgagctcgc gcgtcggcac caggacgaga
      961 gcagtcggcg tggggtcgtt cggtcggacg cgctcgacga tcggaatcgc gaacgcgagc
     1021 gtcttgccgg agccggtcgc ggaacgggcg agcacgtctc gtccggcgat ggcatcccgc
     1081 agcacgagcg actggatcgc gaacgggtcg gataccccta ccctgccgag ggcctcggtc
     1141 acgcgcccgg acacgcccag gtcggcgaac gtggtggtca tgttctctcc ttcttccggc
     1201 gcgtcgagcg cgccgcatct tcggccggct acgcctgcgt gggtgcaccg agcggcgcgg
     1261 ccaggtggat cgcggggagg tttggccttg ccccgagacc ccggccagat gatccggaag
     1321 gaggaacgag gagcgaaacc tcacggacgg cgatgccgtc ctcgacgacc acgataccag
     1381 ctacgcgtca gtggggaagc aggagcagca ggatcatcgc gtagtggcac gccgccgcgg
     1441 ccaccatgaa cgagtgccac acctcgtggt acccgaagat cgacggcgat ggatcgggtc
     1501 gccgcatcgc gaacatgatc gccccggccg tgtagagcag tcccccggcg agcagcagga
     1561 cgagctccgc gcgcgtcatc tcgcgcgcga gctgcgggag ggccacgagg gccagccagc
     1621 cgagcacgat gtagagcgcg gcgctcacca cggagagccc atcggggcga gccatcttca
     1681 aggtcacccc ggccaccgct cccgtccaga tcacggacag gagcacgacc tcccagggcc
     1741 cgccgagcac gagcgcagcg accggcgtgt agctcgccgc gatcagcacg tagatcatcg
     1801 agtggtcgag ccgcttcaac caccgcctcg cgcgttcggt ccaccggccc cggtggtaga
     1861 cggcgctcga gccgaacacg gccgcgaggc tcaccgcgta cacgctcgcg acggcgatcg
     1921 ctgcgggtcc gttcgcgagc aggatcagca ccacgcccgc gggcagcgac acgaagaacg
     1981 cgacctggtg gatccgccct cggaaacggg gcttggggga tgcgaggacc gtggccatgg
     2041 tcgcccatcg tacccgggtg gcatgggcta ccctgcctcg atgacgaacg atctcgtccg
     2101 ccggatcgcc gtcgcccgcg gcgacgagcc cgcggacctg gtcctcaccg gtggtcgcgt
     2161 gctgtccgtg ttcacgggcg agttcctcga ggccgacgtc gcgatcgtcg gcgaacacgt
     2221 tgccggcgtc ggtccgggct acgaggggac cgaacggctc gacgtctcgg acctgatcct
     2281 gctgcccggc ttcatcgacg ggcacatgca cctggagacg accaagctcc ggctcgacca
     2341 gttcgcgcgc gcggcgctcc cgtggggaac caccaccgtc gtcctggacc cgcacgagat
     2401 cgccaatgtg ttcgggatcg acggcgtccg cgcgctgctg cacgacgccg acgaggtccc
     2461 gctcgacgcc tacgtgatgg tctcctcgtg cgtgccggcc tcgccgttcg agtcctcggg
     2521 ggccacgctc acacccgagg acatcgccga gctgctgcgc accgagccgc gggcgatcgg
     2581 gctggccgag atgatggact accctgcggt cgtcgcgggg gtcgaggacg cggtcgccaa
     2641 ggtcgctgcc gccggggacc gtcacatcga cggccacgcc cccggtctct ccggccgctc
     2701 gctgaacgcg tacctcgcgg cgggggttcg gagcgaccat gaatgcacga cgtacgagga
     2761 ggcgctcgag aagcgccgcc tggggatgtg gatcatgatc cgccagggct ccgcggcgcg
     2821 gaatctcgag gcgctcctcc cgctcgtgct cgagcacggg atcaccaact gcctgctctg
     2881 cacggatgat cgagagccgc acgacctgca ggagcacgga cacatcaacg acgtggtccg
     2941 cgaggcggtg gcgctgggct gcccgcccgc ggacgccgtc gcgatggcca cgctcaacgc
     3001 ggcccggtac caccgcctcc acgagcacgg cgccgtcgcg cccggctacc tcgccgacat
     3061 cgtcgccgtg cgagacctcg tgtcgttccg ccccgagcgc gtgtggaagc gcggccggct
     3121 tgtcgccgac ggcgggcggc cctcgccgat cgcgtcgtcg tcgctgcccg gttggatgcg
     3181 ggagagcatg cacgtgcgcg agctcgagcc ggtcgacttc tccgtcgtcg ccgacggggc
     3241 cgtccgcgtg atcggcgtcg atgcggggac gatcgtcacc cgagccctgg tcggcgaacc
     3301 gctgcggcga gacggcaagg cgtgcgcaga cccggggcgc gacctggcga agctcgccgt
     3361 gatcgagcgg caccgggaca cggggcggat cggcgttggt ttcgcgcagg ggttcgggct
     3421 gcaggcgggg gcgctggcat cgagccacgc gcacgacgcc cacaacgtcg tggtggtggg
     3481 catgaccgac gaggagatgg cggcggcggc caaccacctc cgtgcgatcg gcggcggcca
     3541 ggtcgtcgtg atgggggaca gggtcatcgc cgacctgccc tgcccgatcg gcggcctcct
     3601 gtcggacctg acgttcgagg aggtcgcgga tcgcgcccgc gtcctggagg aggcggccgg
     3661 ggagctcggc accacgctcc cgtcgccgtt catggcgatg tcgttcctcg ccctgtccgt
     3721 gatccccgaa ctcaagctca cggaccgcgg cctcgtcgac gtggaccggt tcgagctcgt
     3781 gccactccag gtctgacgac ggcgttcagc gcagcagcga ccggacccgg tcggcgatcg
     3841 cggaggccga gatcccggcc cagtcgcgga gctcctcggg cgtccccgag cccggcatct
     3901 gcgtgacggc gaccttgacg accgtgcccg agagctcccc accgtccgcg agcgccgcga
     3961 ggacggcgtc gccgagccct ccctcgatcc agtggtcctc gatcgtgacg agcagaccgg
     4021 tctgggacgc cgcggcgcgg agcgtctccg cgtcgatcgg cttgatcgtg tagcaatcga
     4081 tcaccctcgc ggcgaccccg tcggcctcga gcgaacccgc ggcctccagc gcctcgtgga
     4141 cggtgatccc cgccccgacg agcgtgaccc ggtccgcgtc cgaggaccgg agggtcttcg
     4201 aaccgccgac cgggaaggcc tcgtcgccct cgtagatcac gggcgtcttc tcgcgggtcg
     4261 tgcggatgaa cgagatcccg ggcaggtcgg ccatcgcccc caccagccgc gcggtcgagt
     4321 tgccgtcgca cggatagagc acggtcgagc cgtggacggc gcgcatcatc gccaggtcct
     4381 cgagcgccat ctgtgacggg ccgtcctctc cgatcgacac gccggcgtgc gaccccgcca
     4441 tcttgatgtt gacgttgctg atcgccgcca tgcggatgaa gtcatatgcc cgcgtgagga
     4501 acgccgcaaa ggtcgatgcg aacggtacga agccgacgac ctgcatgccg acggcgaccg
     4561 cgaccatccg ctgctcggcg atgtacatct cgaagaagcg atccacggcg gtcttcttga
     4621 agtcctcggc atgcgtcgag ttactcacct cggcgtcgag cacgacgacg tcggcgcgcg
     4681 ccgcgccgag cgccgcgagc gcctcgccgt acgccttgcg cgtcgcgacg gcttcgctgt
     4741 atcgcttgag ctcgggcgtc ttcgatggcg cgagtggcgc ggggcgccac gcgggcgggg
     4801 acggcacctc gaaggcgcgc gacggctcgg cgccgatctc cgcgagcgcc tgttttgctt
     4861 gctccgcgtc gagcgccttg ccgtgccaac ccggcttgtc ttcgaggaag gacacgccct
     4921 tgcccttcac cgtgcgggcg acgatcgccg tcgggcgcga tgcggaccgc gcgcgatcca
     4981 gcgcgtcgtc gatctcggcg aggtcgtggc cgtcgatgac gattgcgtcc cagccgaacg
     5041 ccttcacccg cgctgcgtac gcgccggtat cccactccag ctccgtcggc ccgcgctgcc
     5101 cgagacgatt gatgtccagg atggcggtga cgttctcgag gccttcgtgc cccgcgagat
     5161 cgaacgcctc ccagatcgat ccctcggccg tttcgctgtc gccgacgacg acccagacgt
     5221 ggaacggcgc cgcgaggagc cgcttgcccg cgagagcgac accgacaccg atcggcagac
     5281 cctgcccgag cgaaccggtc gcgacgtcga cccacggcag caccggtgtg ggatgacctt
     5341 cgagccgcga gccgaacttg cggaaggtca gaagctcggc atcgtcgatc gcgcccgcgg
     5401 cgcggaacat cgcgtacacg agcggcgagg cgtgaccctt cgaaaagatg aggtggtcgt
     5461 tcgcgagcgc atcaggccgc tcgaagtcgt agcgaaaatg gcccgcgagc aacacggcca
     5521 tgaggtccgc cgcggacagc gacgacgtcg ggtgccccga tccggcggcg gtcgagcagc
     5581 ggatgctgtc ggcgcgcagc tgacgcgcca g
//
LOCUS       DATBRK010000441         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1556252, whole genome shotgun sequence.
ACCESSION   DATBRK010000441 DATBRK010000000
VERSION     DATBRK010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556252"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VIH70_12940"
     CDS             complement(<1..602)
                     /locus_tag="VIH70_12940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570527.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY8117315.1"
                     /translation="MAVARPPATNDPIYDGTRIPSPIPTVLEVDPAIRDTDDILTVNF
                     GPNHPSTHGVLRLIVDLDGEVVVGLHAKVGYVHTGFEKNMEQKSWWKAITYAPRIDYL
                     AYQNNELVFVLAIEKLLGLEVPAKATWMRMLLSELNRIHSHLVWLATGALELGAISMF
                     WYAFREREMILDLSELVAGYRMHTRYFQTGGLAEDIPPGF"
     gene            complement(602..1147)
                     /locus_tag="VIH70_12945"
     CDS             complement(602..1147)
                     /locus_tag="VIH70_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY8117316.1"
                     /translation="MAFEKLPGLVETREAFGETTLVVDPARLVEACTSLRDEHGFDML
                     IDIAATDYLGWGAPGVSAYIGTAAGRNLNRPMTQGLQALPAPKPKRFSLNYHLLAIRQ
                     GARRLRVKVWLDDGEAVPSVVSVWPGADWFEREAWDMMGIPVEGHPHPERLLMDEDWE
                     GHPLRKDYPLGGEPVRFSGEE"
     gene            complement(1192..1542)
                     /gene="nuoB"
                     /locus_tag="VIH70_12950"
                     /pseudo
     CDS             complement(1192..1542)
                     /gene="nuoB"
                     /locus_tag="VIH70_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005239105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
BASE COUNT          252 a          514 c          515 g          261 t
ORIGIN      
        1 tagaagcccg gcgggatgtc ctccgcgagc ccgccggtct ggaagtaccg cgtgtgcatg
       61 cggtagccgg cgacgagctc cgagagatcg aggatcatct cgcgttcgcg gaacgcgtac
      121 cagaacatcg agatcgcccc gagctcgagc gctcccgttg cgagccagac gaggtgcgag
      181 tggatccggt tcagttccga gaggagcatc cgcatccagg tcgccttcgc cgggacttcc
      241 aggccgagca gcttctcgat cgcaagcacg aagacgagct cgttgttctg gtaggcgagg
      301 tagtcgatcc gaggcgcgta ggtgatcgcc ttccaccagg acttctgctc catgttcttc
      361 tcgaacccgg tgtggacata gccgaccttc gcgtggagcc cgaccacgac ctcgccgtcg
      421 aggtcgacga tcaggcgcag cacgccatgc gtcgaggggt gattcgggcc gaagttgacg
      481 gtcaggatgt cgtccgtgtc ccggatcgcg gggtcgacct cgagcacggt tgggatcggc
      541 gaggggatcc tggtgccgtc gtagatcgga tcgttcgtcg cgggtggtcg tgcgaccgcc
      601 atcactcttc tcccgagaag cgcactggct cgccgccgag cgggtagtcc ttccgcagcg
      661 ggtggccttc ccagtcctcg tccatcagca gccgctcggg atgcgggtgg ccttcgaccg
      721 ggatgcccat catgtcccag gcctcgcgct cgaaccagtc cgcccccggc cagacggaga
      781 ccacgctcgg caccgcttct ccgtcgtcga gccagacctt gacacggagt cgccgcgcgc
      841 cctgccggat cgcaagcagg tggtagttga gcgagaaccg cttgggcttc ggagcaggga
      901 gcgcctgcag tccctgggtc atcggccggt tcagattccg ccctgcggcc gtgccgatgt
      961 aggcggagac gcccggagcg ccccagccga ggtagtcggt tgcggcgatg tcgatgagca
     1021 tgtcgaagcc gtgctcatcg cggagggacg tgcaggcctc gacgagccgc gctgggtcga
     1081 cgacgagcgt cgtctccccg aacgcctccc gcgtctcgac cagacccggc aacttctcga
     1141 aagccactac ttggcgaccc cgccgacctc gtaggcggcc ggcacgcccg cctggacgcg
     1201 ctcgtgcagc cggatgatcc cctccatcag gccttccggc ctgggcgggc agccggggac
     1261 gtagatgtcg acggggatga tcttgtcgac gctctggacg acggcgtagt tgttgaacat
     1321 gccgccggag ctcgcgcagg cacccatcgc gatcacccac ttcggctcga gcatctggtc
     1381 gtagatctgc ttgatcggcg ccgccatctt ctgggcgacc cggcccgaga tgatcagcag
     1441 gtcggcctga cgcggcgaag aacggaacgc ctccatcccg aaccgcgcga tgtcatagcg
     1501 cgacgagacg atcgacatca tctcgatcgc gcagcaggcg ag
//
LOCUS       DATBRK010000442         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2272646, whole genome shotgun sequence.
ACCESSION   DATBRK010000442 DATBRK010000000
VERSION     DATBRK010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272646"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VIH70_12955"
     CDS             <1..231
                     /locus_tag="VIH70_12955"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8117317.1"
                     /translation="GGAPPVAFQRVPEAKSVKNRLHVDLAVDDLDDAFAKIVALGGSA
                     VSDILEMSGGYRWRVMGDPEGNEFCIVPRDED"
     gene            complement(239..1417)
                     /locus_tag="VIH70_12960"
     CDS             complement(239..1417)
                     /locus_tag="VIH70_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8117318.1"
                     /translation="MTVRRRLWPIVARRELVERARDRGFQVSTAITLLLLTGVIVISA
                     AVNRPTSFDLVVVGEGSDALGREVRAAADALGIDVIVRELADQAEARRAVDAGETDAA
                     LVDAAQIVVRAAPPDQLVGLIQAVSVRQRSRRALITAGLTPEEVDAALLQAPLPVNAL
                     EPVDDRSRETATVVFVGVLLLYGQLFAYGYWVAAGVVEEKSSRVVEVLLATLRPSHLL
                     RGKILGIGLLGLGQLLLIGLVGLFASASVGSLDFPSGAVATIGLVLAWFVLGFFFYAS
                     LFAVAGSIVTRQEDLQTTMTPLTILILGSFFIGLSATSNPDSTLATVASLLPFSSPLV
                     MPTRIALGEASTWEVVASVAISVGATAAVIPIATTIYSRALLRPGRVRIRQVLRAGRA
                     "
     gene            complement(1414..>1924)
                     /locus_tag="VIH70_12965"
     CDS             complement(1414..>1924)
                     /locus_tag="VIH70_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HEY8117319.1"
                     /translation="AESLSLGNQQRVQLAAALVHRPEVLVLDEPFSGLDPVGVDVLSE
                     LLRRQADDGAPVVFSSHQLELVERLCESVVMIDRGRIVASGRISDLRARDTRRLVRVE
                     VAGAPDRWLDRVPGVRVLEPLAEGAIVELDGASESDVLDAARAAGSVRHFSVVRPSLS
                     EIFRREVAR"
BASE COUNT          318 a          705 c          675 g          226 t
ORIGIN      
        1 ggcggcgcgc cgccggtcgc gttccagcgg gtgccggagg ccaagtcggt caagaaccgg
       61 ctccacgtcg acctcgcggt cgacgacctc gacgacgcct tcgcgaagat cgtcgcgctc
      121 ggcggttcgg ccgtctcgga catcctggag atgtccggcg gttaccgctg gcgcgtgatg
      181 ggcgacccgg agggcaacga gttctgcatc gtccccaggg acgaggactg agccgcgctc
      241 acgcgcgacc ggcgcgcagc acctgccgga tccgaacgcg gccgggtcgc agcagcgcac
      301 gggagtagat cgtggtcgcg atcgggatca cagccgcggt agcgcccacc gagatcgcga
      361 ccgaggccac cacctcccac gtggaagcct ctcccagggc gatcctggtc ggcatcacga
      421 gcggggagga gaagggtagg agcgacgcga cggtcgccag cgtcgagtcc gggttgctcg
      481 tcgcgctcag cccgatgaag aacgagccca ggatcaggat cgtcagcggc gtcatcgtcg
      541 tctggaggtc ctcctgccgc gtgacgatcg atcccgcgac cgcgaacagg ctcgcgtaga
      601 agaagaagcc gagaacgaac cacgccagca cgagcccgat cgtcgcgacc gcgcccgagg
      661 ggaagtccag cgagcccacc gaggccgacg cgaacaggcc caccagcccg atcagcagga
      721 gctgcccgag ccccaggagc ccgatgccga ggatctttcc gcgcagcagg tgcgacggac
      781 gcaacgtcgc cagcagcacc tccaccaccc gcgaggactt ctcctccacc acgccggccg
      841 cgacccagta gccgtacgcg aacagctgtc cgtacagcag gagcacgccg acgaacacca
      901 cggtagcggt ctcgcggctg cggtcgtcca ctggctccag cgcgttgact gggagcggtg
      961 cctgcaggag agcggcatcg acctcctcgg gcgtgagccc cgccgttatg agcgcgcgcc
     1021 gcgagcgctg ccggaccgag accgcctgga tcagcccgac gagctggtcg ggtggcgcgg
     1081 cgcgcacgac gatctgggcg gcgtcgacga gcgcggcatc ggtctctcct gcgtcgacgg
     1141 cacggcgggc ctcggcctgg tccgcgagtt cgcgcacgat gacgtcgatc cccagggcgt
     1201 cggccgcggc gcgtacctcg cgcccgagag cgtcggaccc ctcccccacg acgaccaggt
     1261 cgaagctcgt cgggcggttg acggccgccg agatcacgat gaccccggtc agcagcagca
     1321 gcgtgatcgc cgtcgagacc tggaagcctc ggtcgcgcgc acgctcgacg agctcacgcc
     1381 tcgcgacgat cggccacagg cgccgccgaa cggtcatcgt gcgacctcgc ggcggaagat
     1441 ctcggacagg ctcggcctga cgacgctgaa gtgcctgacc gatcccgcgg cccgcgcggc
     1501 gtcgagcacg tcggactcgc tcgcgccgtc gagctcgacg atcgcgccct ccgccagcgg
     1561 ttcgagcacg cggacgcccg ggacgcggtc gagccatcga tccggagccc ctgccacctc
     1621 gacgcggacc aggcgccggg tgtcgcgggc gcgcaggtcc gagatgcgcc ccgacgccac
     1681 gatccgcccg cggtcgatca tcaccacgga ctcgcagagc cgctcgacca gctcgagctg
     1741 gtggctggag aacacgaccg gagcgccgtc gtcggcctgc cgccggagga gctccgagag
     1801 gacgtcgacg ccgaccgggt ccaggcccga gaacggctcg tccagcacca gcacctccgg
     1861 ccggtgcacg agcgcggccg cgagctggac gcgctgctgg ttgccgagcg agagcgactc
     1921 ggcc
//
LOCUS       DATBRK010000443         5367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_261487, whole genome shotgun sequence.
ACCESSION   DATBRK010000443 DATBRK010000000
VERSION     DATBRK010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261487"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1124
                     /locus_tag="VIH70_12970"
     CDS             324..1124
                     /locus_tag="VIH70_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117320.1"
                     /translation="MAGLAASVLVAALLIGILVIGGGEEGTGARPPRGVDPVEPPPAL
                     GPPTSLEASARPFAVTLRWAAPEGEADQLVVYRDGDRLAPLEPNRTGFVDDTVLPAVH
                     YTYVVEAVADGKASKSDPIRVKTRPAPLGMARLDGTFTVDLRDTSHYGFSSFPGDLQS
                     GWRFKAKCRRGACDVAWKDAQGFALAGELDKTGPRYEGTATTRLASCGDLRGSGTVTV
                     TIEVVKAGSIRDEWRATKIAGTLVERFPAQLGCVASGADYQVTGTLIA"
     gene            1231..1788
                     /locus_tag="VIH70_12975"
     CDS             1231..1788
                     /locus_tag="VIH70_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8117321.1"
                     /translation="MALGESFAGVLEAARVGAEWAWRALYADTAPGLLGYARASRAPD
                     PEDVVGETFLRAVRGVGSFEGDERGFRTWLFVLARNRIMDEHRKLVRRRTEPVPVEVL
                     AEIGPRADAETEAMRALAEDHVRSVLDRLTPDQRDVLLLRILGGLTIEEVSTVVGKKP
                     GAVKALQARGLAAIRDKIDLGAVTL"
     gene            1827..2516
                     /locus_tag="VIH70_12980"
     CDS             1827..2516
                     /locus_tag="VIH70_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117322.1"
                     /translation="MGPDEGFERLLRGETPKTPEERSLGLFLEDVRGAFPTEPSLQEG
                     AQLAAIVQAAQLLADKGEPSVRYATEAGRRAKRASGRPKRRRVIVKLPLMRSLAAKVV
                     AGLVVLMSMFGGLALAGALPGQGSDDDAVEIQNVSDEAPETEAPETEDADDQGEDADD
                     QGVNEDADVDADDQGENEDADVDADDQDENEGADDQGENEDADDQGDDADDQGEDADD
                     QGENEDQGEND"
     gene            complement(2644..3126)
                     /locus_tag="VIH70_12985"
     CDS             complement(2644..3126)
                     /locus_tag="VIH70_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117323.1"
                     /translation="MNEPSLEAVVLYLAEDGIRHLFPTGQQTVAARWDPDLDPHEAIV
                     DAVAELGLQPFMIHSTSWRVERKLIVLTFLVAVERPERIPNAYEDELVARAELARGYA
                     TGPPPHVHLSQVVEHGLRHLAWLVREDGAIHRALADWSRALSEYEPEPFRAFGREPGS
                     "
     gene            complement(3199..3729)
                     /locus_tag="VIH70_12990"
     CDS             complement(3199..3729)
                     /locus_tag="VIH70_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117324.1"
                     /translation="MYLGGPLGWATLILVLREADLEDSRAARADAEDQHLRYEAAMRR
                     DAKLRAIGSEATFFTGLARIDGEFRSVRVAVTPEDFVVLDDWTHLDPDTELARFPRES
                     VTDVVIVDENGNEVADQLLDPIRELETSKEERYAVVLKRHDASGRLPAVSFLFLSGEP
                     ALECRDDYRRFIGPGG"
     gene            complement(3843..4355)
                     /locus_tag="VIH70_12995"
     CDS             complement(3843..4355)
                     /locus_tag="VIH70_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117325.1"
                     /translation="MKVCPHCAEELPDEATVCSNCHKDPALAPALSVPERPDETSLRR
                     LGDVFGPDGVLPTSDQVPAAPTKLLESSEASRIPSKVWMSLILALVWGFALGRIPGLT
                     SLAPSWGTPILQAAGYIAGLILGIRGRADVSPSDRRGQILGNTAIALNGFLLAWDVMF
                     AIQYLLVMRG"
     gene            4781..5044
                     /locus_tag="VIH70_13000"
     CDS             4781..5044
                     /locus_tag="VIH70_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117326.1"
                     /translation="MANTATRDAPDLTLKEPIPAPAGYGFRLDDSYQASTRTYRSEPI
                     PGKTANQAVQEIVDYYIVGLAPEWTVVERKRRRGPWGWAQCAR"
BASE COUNT          912 a         1774 c         1878 g          803 t
ORIGIN      
        1 gcgacgcagg cggccaggtc ctcgtcggcc ggcgcgtcga gcgggaaccg ccacgccggc
       61 cggcgctcgg gcggcgggcc gacgaagcga accccctcgg gcatcaccgc tccgagcacg
      121 cggctcggcg gaaggacttc atcgggcggc gacacatgcc gatggtatct ccggcgtgca
      181 tcggcgccga cgggtacgga cccgcggggg cgactttcga ggaggggtgt gtgcagacac
      241 cgacgaggtc gagcgacctt ccgaatcccg ccgagcccct gtcgccgccg gcacgcccgc
      301 cacgtagatc cagggtcgtt ctggtggcgg gtctcgccgc gtccgtgctg gtcgccgccc
      361 tgctgatcgg gatcctggtc attggaggag gcgaggaggg gacgggcgcc cgaccccctc
      421 gaggcgtcga ccccgtcgag ccaccgccgg cgctcggtcc accgacctcc ctcgaggcct
      481 ccgcgcggcc cttcgccgtc acgctgcggt gggcggcgcc ggagggtgag gccgatcagt
      541 tggtcgtcta ccgggacggg gatcgcctgg cacccctcga gccgaaccgg acggggttcg
      601 tggacgacac tgtcctgccc gccgtccact acacgtacgt cgtcgaagcc gtggcggacg
      661 ggaaggcctc gaagagcgac cccatccggg tgaagacccg gcccgccccg ctcgggatgg
      721 cccggttgga cgggacgttc acggtggacc ttcgcgacac gtcgcactac ggcttttcct
      781 cgttccccgg cgacctccag tcgggctgga ggttcaaggc gaagtgccgc cggggcgcct
      841 gcgacgtcgc atggaaggat gcgcagggtt tcgcccttgc gggggagctc gacaagacag
      901 gcccgcgata cgaggggacg gccacgaccc ggctcgcgtc ctgcggcgac ctgcggggat
      961 cgggaaccgt cacggtcacg atcgaggtcg tcaaggccgg atcgatccga gacgagtggc
     1021 gagcgacgaa gatcgccggc acgctcgtcg aacgcttccc ggcacagctc ggatgcgtcg
     1081 cctcgggagc cgactaccag gtcacgggca cgctgatcgc ctgagctacg ctacccgggc
     1141 cgacgtagtc gggttcagca ccctcattgc cgaactcgcg cgcttcgctc cacctcgtgc
     1201 acccctcgtg ccgatacctg agggagccag atggcgctcg gagagtcatt cgccggagtg
     1261 ttggaggccg cgcgagtagg cgcggagtgg gcctggcgcg ccctgtacgc ggacacggcg
     1321 ccgggcctcc tcgggtacgc gcgcgcgagt cgcgcccccg atcccgagga cgtcgtcggc
     1381 gagacgttcc ttcgggcggt ccgcggggtc ggatcgttcg agggagatga gcgcgggttc
     1441 cgaacgtggc tgttcgtgct cgcgaggaac cggatcatgg acgagcatcg caagctcgtc
     1501 cgtcgccgga ccgagccggt tcccgtcgag gtcctggccg agatcggtcc gcgcgccgac
     1561 gcggagaccg aggcgatgcg tgccctggcc gaggatcacg tccggtccgt gctcgaccgc
     1621 ctcacccccg atcaacgcga cgtcctgctg ctgcggatcc tcggggggct cacgatcgag
     1681 gaggtttcga ccgtggtcgg caagaagccg ggggccgtca aggcccttca ggcacgggga
     1741 ctcgcggcga tccgggacaa gatcgacctg ggggccgtga ccctttgagc gactcgaacg
     1801 ttcctccagt tgagatgagc ggatcgatgg gtcccgacga ggggttcgag cgcctgctcc
     1861 gcggggagac tccgaagact cccgaggagc gatcgctcgg tctgttcctc gaggacgtgc
     1921 gcggcgcctt cccgaccgag ccgtcgctcc aggagggagc gcagctcgcc gcgatcgtgc
     1981 aagccgctca gctcctcgcc gataagggcg aaccgtccgt gaggtacgcc accgaggccg
     2041 gccggcgtgc caagcgcgcg tccggacggc cgaagcgaag gagggtgatc gtgaagctac
     2101 cgctgatgag atcgctggcg gcgaaggtcg tcgccgggct cgtcgtactg atgtcgatgt
     2161 tcggaggctt ggcgttggcc ggagcgctgc cagggcaggg ctccgacgac gacgccgtcg
     2221 agatccagaa cgtgtccgac gaggcgccgg agaccgaggc gccggagacc gaggacgccg
     2281 acgaccaggg tgaggacgcc gacgaccaag gcgtgaacga ggacgccgac gtggacgccg
     2341 acgaccaggg cgagaacgag gacgccgacg tggacgccga cgaccaggac gagaacgaag
     2401 gcgccgacga ccagggcgag aacgaggacg ccgacgacca gggcgatgac gccgacgacc
     2461 agggcgaaga cgccgacgac cagggcgaga acgaagacca gggcgagaac gactagggga
     2521 gcgaggccaa ccggccacct ccgccgagag acgctcgatc gtcttacgag aggtcctacc
     2581 caccaccatc ggtgggtagg acctcagtcg tgggtgggtc gccgcgtcat acgcggcgcg
     2641 tatttaggac ccgggttcgc ggccgaacgc gcggaacggt tccggttcgt actcgctcaa
     2701 cgcgcgcgac cagtccgcga gcgcacggtg gatcgccccg tcctcgcgta ccaaccaggc
     2761 gaggtgccga agcccgtgct cgacgacctg agagagatgg acgtgcgggg gcgggccggt
     2821 cgcgtagcca cgcgcaagct cggctcgcgc gacgagctcg tcctcgtagg cgttcgggat
     2881 ccgctcgggt cgctcgacgg cgacgaggaa ggtcagcacg atcagcttgc gctcgaccct
     2941 ccacgaggtc gaatggatca tgaacggctg aagcccgagt tcggccacgg catcgacgat
     3001 tgcctcgtgc ggatccaggt ccggatccca ccgcgcggct acggtttgct ggcccgtcgg
     3061 gaacagatgc cggatcccgt cctccgcaag gtagagcacg accgcttcga gcgatggttc
     3121 gttcacgctc gaaccctacc gacccgggaa cggcgggtac cgtagccgga cgcaccgcgg
     3181 gatcttcgac agacgcgatc aaccgcctgg cccgatgaag cgacgatagt cgtcacggca
     3241 ctcgagcgcc ggctcaccgg ataggaagag gaacgagacc gccgggagcc ggccgctcgc
     3301 gtcgtgccgc ttcagtacca cggcatagcg ctcctccttg gacgtctcga gctctcggat
     3361 cgggtcgagg agctggtccg cgacctcgtt tccgttctcg tcgacgatca cgacatccgt
     3421 gacgctctcg cgcgggaagc gtgcgagctc ggtgtcggga tcgaggtgcg tccagtcatc
     3481 gagcaccacg aagtcctcgg gcgtcaccgc gacccggacg gagcggaact ccccgtcgat
     3541 gcgcgcgagg cccgtgaaga aggtcgcctc cgagccgatc gctcgcagct tcgcatcgcg
     3601 acgcatggcc gcctcataac ggaggtgctg atcctccgca tcggccctcg ccgcacggga
     3661 gtcctcgaga tcggcctcgc gcagcaccaa gatcagagtc gcccagccga gcgggccgcc
     3721 caagtacatg gaggcaatat cggttgtgcc gccctcatgc atgagcacat gcgttgcccg
     3781 cacgttgagg tacgtgctcg aagcgccggg gggcatgggt ccggcgatgt cggtcggcct
     3841 gatcagcccc tcatgaccaa caaatattga atcgcaaaca tcacgtccca ggccaggaga
     3901 aatccgttca gcgcgatcgc ggtgtttccg aggatctgcc cacgccggtc cgaaggtgat
     3961 acgtctgccc gaccccgtat gccgaggatc aacccggcga tatagccggc cgcctgaagg
     4021 atcggagtcc cccaggatgg agcgaggctc gtcagccccg gaatcctccc caaggcgaac
     4081 ccccagacaa gggcgagaat gagggacatc cacaccttcg acgggatcct cgaagcctcc
     4141 gaggattcaa ggagtttggt aggagcagcg ggaacctggt cagaggtcgg cagaacgccg
     4201 tccggtccga acacgtcgcc gagccgccgc agcgaggtct cgtcgggccg ctcaggcacg
     4261 ctcaaagccg gagccaaggc tggatccttg tggcagttgg agcacaccgt cgcctcgtcc
     4321 ggcagctcct cggcgcagtg cggacacacc ttcacgcttg gtactttcgg cgccgggtcg
     4381 gcgtagctgt agcccgtggc gcgtgccccg ggggcggacg cggaccccga ccaacagttc
     4441 ccgaatgggc gctctcttgc accagggttc gtgaatgtcg cccgagtacc ggcgtcaaca
     4501 ctcaaggcct gagcgttgca gggcgatacc taccgggaag ctcagaccgg gacgggtgaa
     4561 catggcgagc gccgcgagag acgacgcggg cgggagcctc atcggcttcg cgatcgtgtt
     4621 cgtttgttcg tctttcgcgg gttcattccg gcgcctacct gggtctggct cgtcgtcctg
     4681 gccgtaggcg tcctgctcgc cgtcaaggac gggatcagcg ggctggacgt gatccacgct
     4741 tgtgctagcc ggggtcatcg tcgtcggatc tctgacgtct gtggccaaca cggcgacgcg
     4801 cgacgcaccc gatctgacgc tgaaggagcc gatccccgcc ccggccgggt acggcttcag
     4861 gctggacgat tcgtaccagg cttccactcg cacgtatcgg tccgagccga tcccgggtaa
     4921 gacggccaac caagcggtcc aggagatcgt ggactactac atcgtcggtc tggcaccgga
     4981 atggacggtc gtcgagcgga agcgaagacg tgggccatgg ggatgggctc aatgcgcacg
     5041 ctaagttcag acagggcgat acgtcgaacg gcatcggcat ccaggtgttt gcggacctga
     5101 ggggtcggtc ttccaccgtg gttctgatga tccggcgctc aattgcgggg acgacgtgcc
     5161 gggatttccc tccggtgaag tctgctggca gggtccggtt tagcgcctgc tcccggcaac
     5221 ggaagccaac gcgaacgaat gatgttcgtc atgccggcgg tggttcgacg cggagccttc
     5281 cggcctaggc gcgagacagc tcctcgatca tgcccttgcg agcccactcc cactcctcca
     5341 ggaagatcag gagatcggcc atctcct
//
LOCUS       DATBRK010000444         4053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1702154, whole genome shotgun sequence.
ACCESSION   DATBRK010000444 DATBRK010000000
VERSION     DATBRK010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702154"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VIH70_13005"
     CDS             complement(<1..351)
                     /locus_tag="VIH70_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117327.1"
                     /translation="MFTVEQRDALRDRVLGLAEQDERVVAGALVGSLAVDGADRFSDV
                     DLTFGIADHVRVGDVLDDWTRTLIDELDAVHLAELERGPTTYRVFLLPDALQLDLSMT
                     TAARFRPAGPRFRLL"
     gene            complement(400..729)
                     /locus_tag="VIH70_13010"
     CDS             complement(400..729)
                     /locus_tag="VIH70_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117328.1"
                     /translation="MDFEETLAALGFHPATGRTPKGVQVLVAEPNPFLTYTVQAFDDG
                     TALFSWEFAVGEYLRTRGIQFGSDETLNQFMFPREDTRGPQESAWLVEAIERARAQLA
                     SIRFDQP"
     gene            complement(753..1745)
                     /gene="rmuC"
                     /locus_tag="VIH70_13015"
     CDS             complement(753..1745)
                     /gene="rmuC"
                     /locus_tag="VIH70_13015"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY8117329.1"
                     /translation="MTQVGYAVLGLVLGAALGAVVLGTWRRSASTPSTRVEARLEVQA
                     AELRRLADAAVAREGSAEHLRSDLAGARRVLEALALREDERRERDAESWQIVRRLSTV
                     LVGSASKGRAGENVLREHLAQLPPGMLVTDLRVNGRVVEFGLLLPDGRRLPVDSKWSA
                     VAELEALEAADDPNEREALAREVERQVALRAREVAQYLDPSLTAPVAVAAVPDAAYEV
                     LRRAHADAFSRGVVIVPYSTAMPVLLFLYSLAHRFGDADDARSALEEVVSLLDAMQLV
                     LENKVARAATMVSNGVDELRSGLGKARGSIARGRTAAAVGAEPDLAEGVLKAVP"
     gene            complement(1813..3006)
                     /locus_tag="VIH70_13020"
     CDS             complement(1813..3006)
                     /locus_tag="VIH70_13020"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8117330.1"
                     /translation="MPTYVSNVTLFDGRTVKRRAGLLFSDGRVEWVGTHARATRSARA
                     ADEIDGRGKTLTPGLIDCHVHLCFDGSADFAGEAREMTTDAAATVKAVRNAARTIGHG
                     VTTVRDLGGRGDSVIQVARGVERGLLPGPRILAAGRALTSTGGHGHQIGFAREVDGPD
                     GLRRAVREEIRAGATAIKLVATGGVLTPGITHDFTAFTQGELDAAVDEAHSWSRVVGA
                     HAIGPEGIVRAVRAGVDSIEHGSMLSAEGARLMKERGTFHVPTISAIRGIVDHADEVP
                     AYAVEKATSLVELAREGFRRSLRGGVRIACGTDAGTPFNPHGNALVEIVRMVGWGMTP
                     LQAMRSATSNAAALLRLPDVGVVSEGAAADLVLFGANPLEDIRAVLEPALVIKGGELV
                     PRLMS"
     gene            complement(3011..3385)
                     /locus_tag="VIH70_13025"
     CDS             complement(3011..3385)
                     /locus_tag="VIH70_13025"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8117331.1"
                     /translation="MAIVRFDDAPVFDLSGVRIRGFASPARGATETMTYRVELAGGQR
                     VPDHTHDHEEVSQLLSGRWTVTIDGEDSELRPGDTAVIPAGVVHASFTLEGDEAVVLT
                     SMPVGTLLIRGDGERVSPPWSE"
     gene            3583..>4053
                     /locus_tag="VIH70_13030"
     CDS             3583..>4053
                     /locus_tag="VIH70_13030"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEY8117332.1"
                     /translation="MLDVAKQAVEAARAAGADYADGRVVADDSESITVRNQEMEGIDR
                     SSSEGLGIRVLVNGYWGFAATARQEPEEIDRTARLAVEIARAAARLPMEPVVLAPIEP
                     VTGTWHSPMQVDPFNIPLEEKVAMLMEATRRMQSVEGLSFAEGGIDLFRRRTFLA"
BASE COUNT          578 a         1553 c         1368 g          554 t
ORIGIN      
        1 caggagccgg aatcgcggcc cggccggacg gaaccgagcc gccgtcgtca tcgagaggtc
       61 caactgcagg gcatctggca gcaggaacac acggtaggtg gtcggcccgc gctcgagctc
      121 ggcaagatgc accgcgtcga gctcgtcgat cagcgtgcgt gtccagtcgt ccagcacgtc
      181 gccgacccga acgtggtcgg cgatgccgaa ggtgagatcc acgtcggaga agcggtcggc
      241 gccgtcgacg gcgagcgagc ccaccagcgc tccggcgacg acgcgctcat cctgctccgc
      301 gagcccgagc acccggtccc ggagagcatc ccgttgctca acggtgaaca tggggtgcga
      361 tcgtggcccg gcatcggcgg cgctcagggg actccatccc tagggctgat cgaaccggat
      421 cgacgcgagc tgcgcccgcg cgcgttcgat cgcctcgacc agccaggcgc tctcctgcgg
      481 tccgcgggtg tcctcgcgcg gaaacatgaa ctggttcagc gtctcgtccg agccgaactg
      541 gatccccctg gtccgcaggt actcgccgac cgcgaactcc cacgagaaca gcgcggtccc
      601 gtcgtcgaac gcctgcacgg tgtaggtcag gaacgggttc ggctcggcga cgagtacctg
      661 cacgcccttc ggcgtccggc ccgtcgccgg atggaacccc aacgccgcga gcgtctcctc
      721 gaagtccatc gcgggcagcg tacccgcgcg cgttacggca cggccttcag cacgccctcc
      781 gcgagatcgg gttcggcgcc gaccgccgcc gcggtccggc cgcgggcgat cgagcctcgg
      841 gccttgccca gacccgagcg gagctcgtcg accccgttgg agaccatcgt ggccgcgcgc
      901 gcgaccttgt tctcgaggac gagctgcatc gcgtcgagca gtgagacgac ctcctcgagg
      961 gccgaacgag cgtcgtccgc gtccccgaac cgatgcgcga ggctgtacag gaacaggagc
     1021 accggcattg ccgtcgagta cgggacgatc acgacgccgc gcgagaacgc gtcggcgtgc
     1081 gcccgtcgca ggacctcgta cgccgcgtcg gggacggcgg ccaccgccac cggtgcggtg
     1141 agtgacgggt cgaggtactg cgccacctcc cgtgcccgga gcgccacctg gcgctccact
     1201 tcgcgcgcga gtgcctcgcg ctcgttcggg tcgtcggcgg cctcgagcgc ctcgagctcg
     1261 gccaccgcgg accacttgga gtcgaccggc agccgccggc cgtccgggag cagcagcccg
     1321 aactcgacca cccgcccgtt cacgcgcagg tcggtgacga gcatgcccgg cgggagctgc
     1381 gcgaggtgct cgcggagcac gttctcgcct gcgcggccct tcgaggcgct gccgacgagc
     1441 acggtcgaga gccggcggac gatctgccaa gactccgcgt cgcgttcgcg ccgctcgtcc
     1501 tcgcgcaggg cgagcgcctc cagcacccgc cgggcgccgg cgagatccga acggaggtgc
     1561 tcggccgagc cctcgcgagc gaccgcggcg tcggcgagcc gacggagctc cgccgcctgc
     1621 acctcgaggc gcgcctccac ccgcgtggac ggcgtcgagg cagacctgcg ccacgtcccc
     1681 agaaccaccg ccccgagcgc cgcgccgagc acgagtccga gcaccgcgta gccgacctgg
     1741 gtcatccgct cctccttgtc ccccggcagc gtagggaccg ggagtgacaa aacctcagcg
     1801 cagcggcgac ccctacgaca tcaggcgggg gacgagctcg ccgcccttga tcacgagcgc
     1861 gggctcgagc accgcccgga tgtcctcgag cgggttcgcc ccgaacagga caagatccgc
     1921 cgcggcaccc tccgacacca cgccgacgtc gggcagccgg agcagtgcgg cggcgttcga
     1981 ggtcgccgag cgcatcgcct gcagcggggt catcccccac ccgaccatgc ggacgatctc
     2041 gacgagcgcg ttgccgtgcg ggttgaaggg cgtccccgca tcggtgccgc aggcgatcct
     2101 cacgccgccc cggagcgagc gccggaaccc ctcgcgggcg agctcgacga gcgaggtcgc
     2161 cttctcgacc gcgtacgccg gcacctcgtc ggcgtggtcg acgatcccgc ggatcgccga
     2221 gatcgtcggg acgtggaacg tcccgcgctc cttcatcagc cgcgcgccct ccgccgacag
     2281 catcgatccg tgttcgatcg agtccacgcc ggcgcgaacc gcccggacga tcccctcggg
     2341 cccgatcgcg tgcgcgccga ccacgcggct ccacgaatgc gcctcgtcga cggcggcgtc
     2401 gagctctccc tgggtgaacg ccgtgaagtc gtgggtgatc cccggagtga gcacgccgcc
     2461 cgtcgccacc agcttgatcg cggtcgcgcc ggcccggatc tcctcgcgca ccgcgcgccg
     2521 gagcccgtcc ggcccgtcca cctcgcgcgc gaacccgatc tggtgcccgt gcccgcccgt
     2581 gctcgtcagc gcgcggccgg ccgcaaggat cctcggaccg gggagcaggc cgcgttcgac
     2641 gccgcgcgcg acctggatca ccgaatcgcc cctgcctccc aggtcccgaa ccgtcgtcac
     2701 gccgtgaccg atcgtccggg ccgcattccg cacggccttg acggtcgcgg cggcgtcggt
     2761 cgtcatctcg cgcgcctcgc cggcgaagtc ggcactgccg tcgaagcaca gatggacgtg
     2821 gcagtcgatc aaccccggcg tcagggtctt gccgcgtccg tcgatctcgt ccgccgctcg
     2881 cgccgaacgc gtcgctcggg cgtgcgtccc gacccactcg acgcgaccgt ccgagaagag
     2941 cagcccggcc cgtcgcttga cggtcctgcc gtcgaagagc gtgacgttcg agacgtaggt
     3001 cggcacgccc tcactcgctc cagggcggcg agacgcgttc cccgtccccc cggatcagca
     3061 gcgtgccgac cggcatcgac gtgaggacca cggcctcgtc cccctcgagc gtgaacgagg
     3121 cgtggacgac cccggccggg atcaccgccg tgtcgccggg ccgcagctcc gagtcctcgc
     3181 cgtcgatcgt cacggtccag cggcccgaga ggagctggga gacctcctcg tggtcgtgcg
     3241 tgtggtcggg gacccgctga ccgcctgcca gctcgacccg gtaggtcatc gtctcggttg
     3301 ccccgcgtgc cggcgacgcg aacccccgga tccgaacgcc cgacagatcg aacaccggcg
     3361 cgtcgtcgaa ccgcacgatc gccatccctc ttcctcctcg tcccttggct cgtcccttgg
     3421 cctcgtccgt tggctcgccc cgtggctcgt ccgttggcct cggccgccta cgaaccgcgc
     3481 gcatcttctc agaacgccac ccgtgcctcc gaacgcaacc tcccgccctc ccgggtactc
     3541 tttcccggcc catcgtcgcc cttccctgag aggttcgatc ggatgctcga cgtcgcgaag
     3601 caggccgtcg aggcggccag agccgccggt gcggactacg ccgacggccg ggtcgtggcc
     3661 gacgactcgg agtcgatcac cgtccggaac caggagatgg aggggatcga ccgctcgagc
     3721 tcggagggcc tggggatccg ggtactcgtg aacgggtact ggggcttcgc cgccaccgct
     3781 cgtcaggagc ccgaggagat cgaccgcacc gcccggctcg cggtcgagat cgcgagggcc
     3841 gccgcccgcc tcccgatgga gcccgtcgtc ctggctccca tcgaacccgt caccgggacc
     3901 tggcactccc ccatgcaggt ggacccgttc aacatcccgc tcgaggagaa ggtcgcgatg
     3961 ctgatggagg cgacccgccg gatgcaatcg gtcgaggggc tgtcgttcgc cgagggcggg
     4021 atcgacctgt tccgccgtcg gacgttcctc gcc
//
LOCUS       DATBRK010000445         5704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1123164, whole genome shotgun sequence.
ACCESSION   DATBRK010000445 DATBRK010000000
VERSION     DATBRK010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123164"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VIH70_13035"
     CDS             <1..784
                     /locus_tag="VIH70_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY8117333.1"
                     /translation="KAVRERQGYQFAARYRDIDVLMVDDIQFLAKREETQTEFFHTFN
                     ALHQKDRQIVIASDRPPQELGMEERLQSRFRLGLCVDVQPPDLETRIAILQLKAQRDE
                     LHVPDDVIEFIANTFDQNIRELEGALVRVVAWGDLTGQPITSELAEQALEDLLPQAEA
                     EIPPDLILEESARYFGLSVQDLISPNRSRPLTTARHVAMYLMRECSGQSLVKIGDTFG
                     GRDHTTARHGILKVERDMRARTATYRQVQDLSRIIRGRARAG"
     gene            1006..2109
                     /gene="dnaN"
                     /locus_tag="VIH70_13040"
     CDS             1006..2109
                     /gene="dnaN"
                     /locus_tag="VIH70_13040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY8117334.1"
                     /translation="MKFRCDRDALSEALQTVQRGVSSRPGIPALTGVLIEASSEGALT
                     LTTTDLEVSARLSIDVQVGEPGISLVPARLLADTVKSLSDAPVEFETDQSQARIRCAA
                     YEGSLRLLPAEDFPALQPPSGTRIVAQAPRFAEAVGQVARAASRDEARPVLTGILLEV
                     SREGVTLVATDSYRLAVRDLVATAAGEARAIVPERALSEAGRAAQAVEKGEVEVFVDE
                     SQVSFQIGQLMLTSRLIEGEFPNYRQLLPEQYESRLTVSRQQLIDAVRRVGLLARDTS
                     PVRLEFNALGVKLSSSSPDLGQAVEAVEARYEGEDIRAAFNPNYLADGLAAASGESVR
                     LEVRDGLKPGIVRGESDEFTYLVMPVRLPTPVG"
     gene            2117..3211
                     /gene="recF"
                     /locus_tag="VIH70_13045"
     CDS             2117..3211
                     /gene="recF"
                     /locus_tag="VIH70_13045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEY8117335.1"
                     /translation="MRLAWVELRDFRNHVETRLDDLPDALITVAGPNGEGKTNLLEGI
                     AFLFYLQSPRTSSTEPVVRRDSDGPAYARGEVITEEAGRVLVEVEIPGRGANRVQVNR
                     STVRRKRDLRRQVRAAYFGPDDLVIVIGDPSKRRDFLDEAIRSLWPLKESAMTAYERT
                     LRQRNRLLKEWEGRGAPTGLEAWDAELVRNGSALVRLRAEAASRLAPSAEEEYRHLAG
                     YGLACEYAPNVTGEPLEEAFERRLAERRSDELIRRTTLVGPHRDDLSLAVRDLGARSF
                     ASHGEAWAAALCLRLGLASAVADEIRERPVLLLDDPFSALDPRRQRLVGERLADRGQV
                     LVSVADDAHVPEPSSLVLDVSAGKVTARGA"
     gene            3217..3570
                     /locus_tag="VIH70_13050"
     CDS             3217..3570
                     /locus_tag="VIH70_13050"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEY8117336.1"
                     /translation="MPHSKGFAHRRDERTPRATRIGDVVDGLLREQTFARGMPIGRLA
                     SDWASVVGPRLAAESAPVSLERGVLVVAATTGPWGAQVRFLAEQIRERAQTAIGPGSV
                     AKVQVVVRADPRKGL"
     gene            3734..5686
                     /gene="gyrB"
                     /locus_tag="VIH70_13055"
     CDS             3734..5686
                     /gene="gyrB"
                     /locus_tag="VIH70_13055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY8117337.1"
                     /translation="MASTATKRAAATKPGQYGSADIQVLEGVEHVRLRPGMYIGTTSS
                     RGLHHLVYEVVDNSIDEAMAGHCDLIIVRLLEGGGVQVDDNGRGIPIKPIPKAKDRRP
                     AVEVVLTTLNAGGKFGGEGYRISGGLHGVGVSVVNALSEKLVVQVMREGASWTQTYAR
                     GKATTRLTKGKATRKTGTIITFWPDPEIFVEGVEFDSRMLAERLQEHAFLTKGVEIQL
                     IDEREGKPDKQVFKASGGIADFVKHLATGKDSIHSKIISMEAREADSEADVALQWTNG
                     YSESIHSFANTINTHEGGMHEEGLKKALTNVVNRYARSKQLLKDKEDNLLGEDVREGL
                     IAIVSVRLREPQFEGQTKTKLGNTTMRSLVETTVNEHLMTWLEEHPGDAKRIVTKSSQ
                     AARARLAAKQARDLTRRKSFLEGGGLPGKLADCQLTDPSATELFIVEGDSAGGSAKQA
                     RDPATQAILPIRGKILNVEKSRLHKILENAEIQALITAIGTGVGDEEFDVSKARYHKI
                     VLMADADVDGAHIQTLLLTFLFRYMRELIDAGYVYLAKPPLYLLKEGRQELYFYTERE
                     WDAYRKDVDGQRKLEPQRFKGLGEMDAAQLWETTMNPEQRTLLRVTLEDAATADRLFG
                     ILMGEDVASRKEWIEDNAADVQNLDV"
BASE COUNT          948 a         1883 c         2090 g          783 t
ORIGIN      
        1 caaggcggtg cgcgaacgac agggctacca gttcgccgcg cggtaccgcg acatcgacgt
       61 gctcatggtc gacgacatcc agttcctcgc caagcgcgag gagacccaga ccgagttctt
      121 ccacacgttc aacgcgctgc atcagaagga ccgccagatc gtcatcgcgt cggaccgccc
      181 gccgcaggag ctcggcatgg aggaacgcct ccagtcgcgg ttccgcctcg gcctgtgcgt
      241 ggacgtgcag ccccccgacc tggagacccg catcgcgatc ctgcagctga aggcgcagcg
      301 cgacgagctc cacgtccccg acgacgtgat cgagttcatc gccaacacgt tcgaccagaa
      361 catccgcgaa ctcgagggcg cgctcgtgcg ggtggtggcg tggggcgacc tcaccgggca
      421 accgatcacc agcgagctcg cggagcaggc gctcgaggac ctgctgcccc aggccgaagc
      481 cgagattccg ccggatctga tcctggagga gagcgctcgc tacttcggcc tgtcggtcca
      541 ggatctgatc tcgccgaacc gttcccggcc gctcacgacg gcccgccacg tcgcgatgta
      601 cctcatgcgc gaatgcagcg ggcaatccct ggtcaagatc ggcgacacgt tcggcgggcg
      661 tgaccacacg acggcccgcc acgggatctt gaaggtcgag cgggacatgc gggctcggac
      721 ggccacctac cgccaggtcc aggatctgtc gcgcatcatc cgcggccggg cccgggcggg
      781 atgagcgccc atccgagccg tggggagggg cctgtgggga acgcgggtgg cgcgggggac
      841 ggggccgggc ggagccgggg cgtgtccaca aattccccca gccggtccac atgtcctacg
      901 ggcccgctac gctcggagaa cgggctggta tccccgttgt ccccagcctc tactactact
      961 gaaagaagtt agagatgaac caagaagcag tcgggaggtg gacacgtgaa gtttcggtgt
     1021 gaccgcgacg cgctgtccga ggcgctgcag acggtccaac gcggcgtctc gtcgaggccc
     1081 gggatcccgg ccctcaccgg ggtgttgatc gaggcgtcgt ccgagggcgc gttgacgctc
     1141 accacgacgg acctggaggt ctcggcccgg ctatcgatcg acgtgcaggt gggggagccg
     1201 gggatctcgc tcgttccggc gcggttgctc gccgacaccg tgaagagcct gtcggatgcc
     1261 cccgtcgagt tcgagaccga ccagtcgcag gcccggatcc ggtgcgcggc gtacgagggt
     1321 tcccttcggc tgctgccggc cgaggacttc ccggccctgc agccaccgtc ggggacgcgg
     1381 atcgtggcac aggcgcctcg gttcgcggag gcggtcgggc aggtggcgcg ggcggcgtcg
     1441 cgcgacgagg cccggcccgt actgacgggg atcctgctcg aggtgagccg cgagggcgtg
     1501 acgctggtcg cgaccgactc gtaccgactc gccgttcggg acctggtggc gacggccgcc
     1561 ggcgaggcgc gggcgatcgt cccggagcgc gccctgtcgg aggccggccg cgccgcgcag
     1621 gccgtcgaga agggcgaggt cgaggtgttc gtcgacgaga gccaggtctc gttccagatc
     1681 gggcagctga tgctcacgtc gcggttgatc gagggcgagt tcccgaacta ccgccagctc
     1741 cttccagagc agtacgagag ccgcctcacg gtctcgcggc agcagctgat cgacgcggtg
     1801 cgcagggtcg ggctgctcgc ccgcgacacg tccccggtcc ggctcgagtt caacgcgctc
     1861 ggcgtgaagc tctcgtcgtc gtcgccggat ctcggtcagg cggtcgaggc ggtggaggcc
     1921 cggtacgagg gcgaggacat cagggcggcg ttcaacccga actacctcgc cgacggcctc
     1981 gccgccgcgt cgggcgagtc cgtgcggctc gaggtgcggg acggtctgaa accggggatc
     2041 gtccgcggcg agtcggacga gttcacgtac ctcgtgatgc cggtgaggct gccgacgccg
     2101 gtcggttgac gacggggtgc ggctcgcgtg ggtcgaactc cgggacttcc gcaaccacgt
     2161 cgagacgcgc ctggacgacc tccccgacgc gctgatcacg gtcgccggcc cgaacggcga
     2221 gggcaagacc aacctgctcg aggggatcgc cttcctgttc tacctccagt cgccgcgcac
     2281 gtcgtcgacg gaaccggtgg tgcggcgtga ttcggacggc cccgcgtacg cgcgaggcga
     2341 ggtcatcacc gaggaggcgg gcagggtgct ggtcgaggtc gagatcccgg gacgaggcgc
     2401 caaccgcgtg caggtgaacc gatcgacggt gcggcgcaag cgcgacctgc gccggcaggt
     2461 ccgcgcggcg tacttcggtc cggacgacct cgtgatcgtg atcggggatc cgtcaaagcg
     2521 acgcgacttc ctcgacgagg cgatccggtc gctgtggccg ctcaaggagt ccgcgatgac
     2581 ggcgtacgaa cgcacgctgc gacagcgcaa ccggttgctc aaggaatggg agggacgcgg
     2641 tgcaccgacc gggttggagg cctgggacgc cgagctggtc aggaatggaa gcgcgctggt
     2701 ccggctccgc gccgaggccg catcgcggct cgcgccgtcg gcggaggagg agtaccgcca
     2761 cctcgcgggg tacggcctcg cctgcgagta cgctccgaac gtgaccgggg agccgctcga
     2821 ggaggcgttc gaacggcggc tggccgaacg ccggagcgac gagctgatcc ggcggaccac
     2881 cctggtcggc ccgcatcgcg acgacctgtc gctcgcggtt cgggacctcg gcgcccggag
     2941 cttcgcgagc cacggggagg cctgggcggc ggcgctctgc ctgcggctgg ggctcgcgag
     3001 cgcagtcgcg gacgagatcc gggaacgccc ggtgctgctc ctggatgacc ccttctcggc
     3061 cctggacccg cgccggcagc ggctggtcgg ggagcggctg gcggaccgcg gacaggtcct
     3121 cgtcagcgta gccgacgacg cccacgtgcc cgaaccctcg tcgctcgtcc tcgacgtgtc
     3181 ggccggtaag gtcaccgcgc ggggggcgtg agcgggatgc cacactcgaa ggggttcgcg
     3241 caccgcaggg acgagcggac cccgagggcg accaggatcg gcgacgtcgt cgacggcctc
     3301 ctgcgcgagc agacgttcgc gcggggcatg ccgatcggcc ggctcgcgtc ggattgggcg
     3361 tcggtggtgg gcccccggct ggccgcggag tccgccccgg tgagcctcga acgcggcgtc
     3421 ctggtggtcg ccgcgaccac cggaccgtgg ggcgcgcagg tgcggttcct ggccgaacag
     3481 atccgcgaac gggcccagac ggcgatcggc cccgggagcg tcgccaaggt gcaggtggtc
     3541 gtgcgggccg acccgcggaa aggcttgtag cgcaaggcgt tcgcggccat gctgcggacc
     3601 tcggatgggg ggtcgaaacg cgtccggcgt cacccccgat tggtagaatc tagggagtag
     3661 ttccgcggtt ccgtcggccc ccgagacgcg tcgtctcgag tccggcgcgc ccgcgttcca
     3721 cgacggaggt gaagtggcaa gcacggcaac caagcgggcc gcggcgacca agcccggaca
     3781 gtacggatcg gcggatatcc aggtcctcga gggcgtcgag cacgttcggc tccgccccgg
     3841 gatgtacatc ggcaccacgt cgagcagggg tctgcaccat ctcgtctacg aggtggtcga
     3901 caactcgatc gacgaggcga tggccggtca ctgcgacctg atcatcgtgc gcctgctcga
     3961 gggtggcggc gtccaggtcg acgacaacgg gcgcggcatc cccatcaagc cgatacccaa
     4021 ggccaaggac cggcggccgg ccgtcgaggt cgtcctcacg accctgaacg ccgggggcaa
     4081 gttcggaggc gagggctacc ggatctcggg tgggctccac ggcgtcgggg tgtccgtggt
     4141 gaacgccctg tcggagaagc tggtcgtcca ggtgatgcgc gagggcgcct cgtggacgca
     4201 gacctacgcg cgcggcaagg cgaccaccag gctcaccaag ggcaaggcga ctcgcaagac
     4261 cggcacgatc atcacgttct ggcccgaccc ggagatcttc gtcgagggcg tcgagttcga
     4321 cagccggatg ctcgcggagc gcctccagga gcacgccttc ctcaccaagg gcgtcgagat
     4381 ccaactgatc gacgagcggg agggcaagcc ggacaagcag gtgttcaagg cgtcgggcgg
     4441 catcgcggac ttcgtgaagc acctcgccac cgggaaggac tcgatccaca gcaagatcat
     4501 ctcgatggag gcgcgcgagg ccgactcgga ggcggacgtg gccctgcaat ggaccaacgg
     4561 ctactcggag tcgatccact ccttcgccaa cacgatcaac acccacgagg gcggcatgca
     4621 cgaggagggc ctgaagaagg cgctcaccaa cgtggtcaat cggtacgcgc gcagcaagca
     4681 actgctgaag gacaaggagg acaacctgct cggcgaggac gtgcgcgagg ggctgatcgc
     4741 gatcgtgagc gtccgcctgc gcgaaccgca gttcgagggc cagaccaaga ccaagctcgg
     4801 caacacgacg atgcgctcgc tcgtcgagac gaccgtgaac gagcacctga tgacgtggct
     4861 cgaggagcac cccggcgacg ccaagcggat cgtcacgaag tcctcgcagg ccgcgagggc
     4921 ccgcctggcg gccaagcagg cacgcgacct gacacgtcgc aagtcgttcc tggagggcgg
     4981 tgggctgccc ggcaagctgg ccgactgcca gctgaccgac ccgagcgcca ccgagctgtt
     5041 catcgtcgag ggcgactcgg ccgggggttc ggccaagcag gcgcgcgacc cggcgaccca
     5101 ggcgatcctg ccgatccgcg gcaagatcct gaacgtcgag aagtcgcggc tgcacaagat
     5161 cctcgagaac gccgagatcc aggcgctgat cacggccatc ggcaccggcg ttggggacga
     5221 ggagttcgac gtctcgaagg cgcgctacca caagatcgtg ctgatggcgg acgccgatgt
     5281 ggacggcgcg cacatccaga cgctgttgct gacgttcctg ttccggtaca tgcgcgagct
     5341 gatcgacgcc ggctacgtgt acctggccaa gcccccgctg tacctgctga aggagggcag
     5401 gcaggaactg tacttctaca ccgagcgcga gtgggacgcg taccgcaagg acgtcgacgg
     5461 ccagcgcaag ctggagccgc agcggttcaa gggcctcggc gagatggacg ccgcgcagct
     5521 gtgggagacg acgatgaacc ccgagcagcg cacgctgctg cgggtgacgc tcgaggatgc
     5581 ggccacggcg gaccggctct tcggcatcct catgggtgag gacgtcgcat cccgcaagga
     5641 gtggatcgag gacaacgcgg ccgacgtgca gaacctggac gtctaggccg aacgatcggg
     5701 gggg
//
LOCUS       DATBRK010000446         6561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1277418, whole genome shotgun sequence.
ACCESSION   DATBRK010000446 DATBRK010000000
VERSION     DATBRK010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277418"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VIH70_13060"
     CDS             complement(<1..834)
                     /locus_tag="VIH70_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8117338.1"
                     /translation="MSTDVHQDGPVLLAEDLGPVRRLTLNRPASLNALNDELMDALEA
                     AFDAAAGEEDVRVVVLRGAGRAFCAGYDLNQDAEEGTKDAAEWHRELDRDTKRLLRIL
                     EHPKPVIASVHSYCLAGGTDLMLACDLAVAADDAVFGYVDVRFGSGVVSMFLPWVVGV
                     RAAKELVLTGQDRIPAAEALRIGLVNRVVPRDELDVATMALADEIATNEPFVVRTMKA
                     SINRVWQLAGLRAALDANTELDVMIETANLPARDEFRRITQEEGLKAAIAWRDARYRG
                     GG"
     gene            complement(915..2111)
                     /locus_tag="VIH70_13065"
     CDS             complement(915..2111)
                     /locus_tag="VIH70_13065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8117339.1"
                     /translation="MGAGFAGLAAALDLHDAGLSVTVLEARDRVGGRVLSVELENGEI
                     AELGAEWIMPDDDVLRATIDRLGLTASEAGVDYLRREPRGDGAVTMAQLDAFLEAADA
                     HLATVDPAERSVGEALDLVPGDDRARAVARARLTGTFASELSLVAWRPGWHAGKLAAE
                     PAVYRRMERGNRSIADAAAAELPEVRLGRTATAVRQGAAGVEIDAGETIRAEAAVVAV
                     PVRLVTELVFDPPLEADQREAFDRLPMGVASKLAIALDGEPERCAIQCADLPFWLWVA
                     DGSNGTRRVVTSFAGSQIPQGPLETATGDPRAWVRRLVELAPGLRPSGAAVMKVWAED
                     RLARGAYSAWDGASLGRRHLFERMHGAVAFAGEHTAGEHSGTMEGALRSGRGAASQVL
                     ERLGRV"
     gene            2260..3585
                     /locus_tag="VIH70_13070"
     CDS             2260..3585
                     /locus_tag="VIH70_13070"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8117340.1"
                     /translation="MTRDPRQAHGHDLSRRDFLKRTAGAAVAIPAAAEILAACTKPGT
                     TSSTGSLPYQIARPENPVTLPMNGDPIPSDTPIEQGAELQILNWSQYMWKYVLNQFVE
                     SHQADGITFTAPSTFQNMDEGVAKLQAGQKADVFFPTIDVMGKLVAADLLQPLNHDLI
                     PALQKDNWKVFQDPYYDQGWRYSVPYTIYTTGIGYRRDHIPDDVIHGMDNPWEILWDP
                     QYSGKVGAYPSYRDVMAIALMKNGITDMNTSKASDLETVRKDLLALTEDVDVRISYSG
                     AYAKLPKDVFWLMTAWSGDMVAGWGYAPPYTEQAYENIGYWFPSDRKGPVDNDLIAIP
                     KNAEHPALGHAFINFWLEYQHAMDNFSWNGYQPPQNQADPSTLTTTEGLYSKISNWAA
                     PAMYVSPWMPDAVVRQEDFAVGYREGPLTPDVDDHWHDVWEEFVAGAKT"
     gene            3572..4519
                     /locus_tag="VIH70_13075"
     CDS             3572..4519
                     /locus_tag="VIH70_13075"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8117341.1"
                     /translation="MQKPSEPTRRSRRRARRVARGRAQYPRWYWPSFALPGILWLLIL
                     FILPLYTVVSIAFGTVDPIFRGPLPVYQPWWWSAEHFGETLDKVFGIFEPVYVHTFVY
                     VVVASAVCLVLGYAVAYYVARFGGRHKGVLLVLLISPFWISYLMRMLAWINLLDNDGY
                     INKFLMFANVVNHPIGWLEGRPLTVIMGLVYGYIPYMILPLYGFLDRIDSSMLESGRD
                     LGGSRISTFWRVTLPLSKPGILAGLIIVSLPMFGDYYTNNLLSGSPRTTMIGNLIDEA
                     YNTTGQGGEMASLVLILMVLLILPMLFYLRSTRRASEAT"
     gene            4516..5436
                     /locus_tag="VIH70_13080"
     CDS             4516..5436
                     /locus_tag="VIH70_13080"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8117342.1"
                     /translation="MTQGTIARPAPVRPAVVPARPSRWWRDPWRKPRILQTVTWAYLI
                     WSLVPLTIAIAISFNGSRSNSILHGFSLQWWWSDPEDSLWHDPELHAALFQSLRLGIL
                     TVLIAVPLGVSFALGIDRWHGRPATGATFLMLLSFVLPEIVLGISLFLVFQHLLTFVE
                     LGTTAQILGLVTYQVSYPVIIVRARLLTIGPEYEEAASDLGAPPRQAIRRVLLPLLYP
                     AIFASAALVFADTIDDFVTVKYLSGPATSEPLSVKIYNFTRAEPTPAVNAAATFLLVT
                     TTVAIVLGYFAYRRATRGQRTGGLSEFANV"
     gene            complement(5433..>6561)
                     /locus_tag="VIH70_13085"
     CDS             complement(5433..>6561)
                     /locus_tag="VIH70_13085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8117343.1"
                     /translation="RVCSAVMAGGEVVLVDLVKRFADVTAVAGINLSMPSGEFFSLLG
                     PSGCGKTTTLRLIAGFERPDEGQILLDGVDMVQTPPHKRNVNTVFQNYALFPHLTVAE
                     NVAFGLRYKDVSKQEANQKVADALELVRLQGLERRRPSQLSGGQQQRVALARALILNP
                     AVLLLDEPLGALDAKLRKALQIELKALQEEIGITFVYVTHDQEEALTMSDRLAVMSNG
                     RVEQIGSPSDVYEEPTTAYVADFLGVSNLMDATCDGADAGGRGKVRLGEFELAAGHGD
                     TDAHGDVKIVIRPERVQLEETGTTGENRVPGMVERVVYVGSIMQVIVHLAPGQTLQAW
                     VQNQGEGLPYGQGHPVSVHLPSDALRILVDTSVPAPAEVPS"
BASE COUNT         1016 a         2364 c         2124 g         1057 t
ORIGIN      
        1 ccccccgccc cgataccgcg cgtcccgcca ggcgatcgcg gccttcagac cctcctcttg
       61 ggtgatccgc cggaactcgt cgcgggccgg caggttggcg gtctcgatca tcacgtcgag
      121 ctcggtgttg gcgtcgagcg cagctcgcag gcccgcgagc tgccacacgc ggttgatcga
      181 cgccttcatc gttcggacga cgaacggctc gttcgtcgcg atctcgtccg cgagcgccat
      241 cgtcgcgacg tcgagctcgt cgcgcggcac gacccggttc accaggccga tcctgagcgc
      301 ctcggcggcc gggatacggt cctgcccggt caggacgagc tccttcgcgg cgcggacgcc
      361 gaccacccat ggcaggaaca tcgagaccac acccgagccg aaccggacgt cgacgtaccc
      421 gaacaccgcg tcgtccgccg ccaccgccag gtcgcacgcg agcatcaggt cggtcccgcc
      481 ggcgaggcag taggagtgca cggacgcgat cacgggcttg gggtgctcga ggatccgcag
      541 gagccgcttc gtgtcgcggt ccagctcgcg gtgccattcg gccgcgtcct tggtgccctc
      601 ctcggcgtcc tggttcaggt cgtagccggc gcagaacgcg cgaccggcgc cccgaaggac
      661 gacgacgcgg acgtcctcct ccccggccgc cgcatcgaac gccgcctcga gcgcgtccat
      721 caattcgtcg ttcaacgcgt tgagcgatgc gggccggttc agggtcagcc ggcggaccgg
      781 gccgaggtcc tccgcgagca ggacgggacc gtcctggtgc acgtcggtgc tcatcgcggc
      841 gtcagactcc ggtccgggtc gagggtgcgg cgcatgatac acggccgcct gaacggcgct
      901 tcaatcgcga ggggtcacac gcggccgagg cgctcgagga cctgtgaggc ggcgccccgg
      961 ccgctgcgca gcgccccctc catcgtgcct gagtgctcgc cggcggtgtg ctcgccggcg
     1021 aacgccacgg caccgtgcat gcgttcgaac aggtgccggc ggccgagcga ggcgccgtcc
     1081 cacgccgagt acgcgccgcg cgccagccgg tcctcggccc agaccttcat cacggccgcc
     1141 cccgagggcc gcagcccggg cgcgagctcc acgagccggc gcacccacgc tcgtgggtcg
     1201 cccgtcgcgg tctcgagtgg cccctgcggg atctggcttc ccgcgaacga ggtcacgacg
     1261 cggcgcgtgc cgttcgagcc gtccgccacc cagagccaga acgggaggtc cgcgcactgg
     1321 atcgcgcagc gctcgggctc tccgtcgagc gcgatcgcga gcttggaggc aacgcccatc
     1381 ggcaggcgat cgaacgcctc ccgctggtcg gcctcgagcg gcgggtcgaa cacgagctcg
     1441 gtgaccaggc gcaccggcac ggcgacgacg gccgcctcgg cgcggatcgt ctcgcccgcg
     1501 tcgatctcga cgccggccgc accctgccgg accgccgtcg cggtgcggcc cagcctgacc
     1561 tcgggcagct cggcggccgc ggcgtcggcg atcgatcggt tcccccgctc catgcgccgg
     1621 tagacggcgg gctcggcggc cagcttgccg gcgtgccagc ccggccgcca cgcgacgagc
     1681 gacagctcgc tcgcgaacgt gcccgtgagg cgcgccctcg cgacggcgcg cgcccgatcg
     1741 tcgcccggca cgaggtcgag cgcctcgccc accgaccgct cggcgggatc cacggttgcc
     1801 aggtgcgcgt ccgcggcctc gaggaaggcg tcgagctggg ccatcgtcac cgccccgtcg
     1861 ccccgaggtt cccgccggag atagtcgacg ccggcctcgc tcgcggtgag gccgagccgg
     1921 tcgatcgtgg cccgaaggac gtcgtcgtcc ggcatgatcc actcggcgcc gagctcggcg
     1981 atctcgccgt tctcgagctc gaccgagagc accctgcccc ccacgcggtc gcgcgcctcg
     2041 agcacggtca ccgaaagccc cgcgtcgtgg aggtccagtg ccgcggccag gccggcgaac
     2101 ccggccccca cgatcacaac gttcacattc cgcacgcacc cacgatagat tggcttgcgt
     2161 acccagattg gcttgcgtac cgcgagcgcc ctgtggtacc caggcctttg aacggcgctt
     2221 caaagacgcg ctggagcgaa cccgaggagg cgacgagcca tgaccaggga tcctcgccag
     2281 gctcacggtc acgatctgag tcggcgcgac ttcctcaaga ggacggccgg ggcagcggtc
     2341 gcgatcccgg ccgcagccga gatccttgcc gcgtgcacca agccgggaac gacatcctcg
     2401 acgggcagcc ttccctatca gatcgcccga cccgagaacc cggtgacgct gccgatgaac
     2461 ggggacccga tcccgagcga cactccgatc gagcagggcg ccgagctcca gatcctgaac
     2521 tggtcgcagt acatgtggaa gtacgtgctg aaccagttcg tcgagtccca tcaggccgac
     2581 ggcatcacct tcacggcacc gtcgacgttc cagaacatgg acgagggcgt cgcgaagctg
     2641 caggccggcc agaaggcaga cgtcttcttc ccgacgatcg acgtgatggg caagctggtc
     2701 gcggccgacc tcctccaacc gctcaaccac gacctaatcc cggcgctgca gaaggacaac
     2761 tggaaggtgt tccaagaccc gtactacgac caggggtggc ggtactccgt cccctacacg
     2821 atctacacga ccgggatcgg gtatcggcgc gaccacatcc ccgacgacgt gatccacggc
     2881 atggacaatc cgtgggagat cctgtgggac ccgcaatatt cggggaaggt cggcgcgtac
     2941 ccgagctacc gcgacgtgat ggcgatcgcg ctgatgaaga acgggatcac cgacatgaac
     3001 accagcaagg catcggacct ggagaccgtc cgcaaggacc tgctcgcgct cacggaggac
     3061 gtcgacgtgc ggatctccta cagcggtgcc tacgcgaagc tcccaaagga cgtgttctgg
     3121 ctcatgacgg cgtggtcggg ggacatggtg gccggctggg ggtacgcacc cccctacacg
     3181 gagcaggcgt acgagaacat cggctactgg ttcccatccg atcgcaaggg tccggtcgac
     3241 aacgacctga tcgcgatccc gaagaacgcc gagcaccctg ccctcgggca tgcgttcatc
     3301 aacttctggc tcgagtacca acacgcgatg gacaacttct cctggaacgg gtaccagccc
     3361 ccgcagaacc aggcggatcc gagtacgctc accacgaccg agggcctgta ttccaagatc
     3421 tccaactggg cggccccggc gatgtacgtg tctccatgga tgcccgacgc ggtggttcgg
     3481 caggaggact tcgccgtcgg ttaccgggaa gggccgctca cacccgacgt cgacgatcac
     3541 tggcacgacg tctgggagga attcgtcgcc ggtgcaaaaa cctagcgagc ccacccggcg
     3601 ttcgcgtcgc cgggcgcgcc gcgtcgcccg tgggcgcgct cagtatccac gctggtactg
     3661 gccgtcgttc gccctcccag ggatcctttg gctcctgatc ctgttcatcc tgcccctgta
     3721 caccgtcgtc tccatcgcgt tcggcaccgt cgacccgatc ttccggggtc ccttgccggt
     3781 ctatcagccg tggtggtgga gcgccgagca cttcggagag acgctggaca aggtcttcgg
     3841 catcttcgag ccggtgtacg tccacacgtt cgtgtacgtg gtcgtggcga gcgccgtctg
     3901 tctggtgctc ggctatgcag tcgcgtacta cgtggcccgc ttcggcggca ggcacaaggg
     3961 cgtgttgctc gtcctattga tctccccgtt ctggatcagc tacctgatgc ggatgctcgc
     4021 gtggatcaac ctgctggaca acgacgggta catcaacaag ttcctcatgt tcgccaacgt
     4081 cgtgaaccac ccgatcgggt ggctcgaggg ccggccgctc accgtgatca tgggtctcgt
     4141 ctatgggtac atcccgtaca tgatcctgcc cctctacggc ttcctggatc gcatcgactc
     4201 cagcatgctg gagtccggac gggaccttgg agggagccgg atcagcacct tctggcgagt
     4261 gaccctcccc ctgtccaaac ccggcatcct ggccgggctg atcatcgtct cgctgccgat
     4321 gttcggcgac tactacacga acaacctctt gtccggctcg cccaggacga ccatgatcgg
     4381 gaacctcatc gacgaggcct acaacacgac cggccagggc ggcgagatgg cctcgctcgt
     4441 cctcatcctc atggtgctgc tgatcctgcc gatgctcttt tacctgcgca gcacgcgccg
     4501 cgcttcggag gccacgtgac gcaggggacc atcgcgcggc cagcgcccgt ccgtccggcc
     4561 gtcgtccccg cgcgacccag tcgctggtgg cgcgatccgt ggcgcaagcc gaggatcctg
     4621 caaacggtca catgggccta cctgatctgg tccctggtcc cgctgacgat cgcaatcgcg
     4681 atctcgttca acggcagtcg gtccaacagc atcctgcacg ggttcagcct tcagtggtgg
     4741 tggtcggacc cggaggactc gctctggcac gaccccgagc tccacgcggc gctgttccag
     4801 agcctgcgac tcgggatcct gacggtgctc atcgccgtcc cgctgggcgt gtcgttcgcg
     4861 ctcgggatcg atcgctggca cggccgcccg gcaacgggtg ccacgttcct gatgctgctc
     4921 tcgttcgtgt tgcccgagat cgtcctgggc atctcgttgt tcctcgtgtt tcagcacctg
     4981 ttgacgttcg tcgaactggg cacgacggcg cagatcctcg gcctggtcac ctaccaggtc
     5041 tcctacccgg tgatcatcgt gcgggcccga ttactcacga tcggcccgga gtacgaggag
     5101 gccgcctccg atctcggcgc gccaccacgg caggcgatcc gtcgcgtgct gcttcccctg
     5161 ctctacccgg cgatcttcgc gagcgcggcg ctcgtgttcg cggacacgat cgacgacttc
     5221 gtgacggtga agtatctgtc cggcccggcg accagcgagc cgctgtccgt caagatctac
     5281 aacttcacgc gcgccgaacc caccccggcc gtcaacgcgg cggccacgtt cctcctcgtg
     5341 acgacgacgg tcgcgatcgt gctcgggtac ttcgcgtacc ggcgagcgac caggggacaa
     5401 cggacgggag gcctctccga gttcgcgaac gtctagctcg ggacctccgc cggtgccggc
     5461 acgctcgtgt cgaccaggat ccggagcgca tcggacggca ggtggaccga cacgggatgc
     5521 ccctgcccgt acggcagtcc ctcgccctgg ttctgcaccc aggcctgcag cgtctgaccg
     5581 ggcgcgaggt gcacgatcac ctgcatgatc gagccgacgt acacgacgcg ctcgaccatc
     5641 ccggggaccc ggttctcgcc ggtcgttccc gtctcctcca gttgcacccg ctcgggtcgg
     5701 atcacgatct tgacgtctcc gtgcgcgtcc gtgtcgccgt gcccggccgc cagctcgaac
     5761 tcgccgagcc gtaccttccc ccgcccgccg gcgtctgcgc cgtcgcacgt cgcgtccatc
     5821 aggttcgaca cacccaggaa gtcggcgacg taggccgtgg tcggctcctc gtagacgtcc
     5881 gagggggagc cgatctgctc cacccgcccg ttgctcatca ccgcgaggcg gtccgacatc
     5941 gtgagcgctt cctcctggtc gtgggtcacg tagacgaacg tgatgccgat ctcctcctgg
     6001 agcgccttga gctcgatctg cagcgcctta cggagcttgg cgtcgagcgc gccgagcggc
     6061 tcgtccagca gcagcacggc cgggttcagg atcagcgccc gggcgagcgc cacccgctgc
     6121 tgttggccgc ccgagagctg cgagggccgc cggcgctcca ggccctggag ccggaccagc
     6181 tcgagcgcgt cggccacctt ctgattggcc tcctgcttgg agacgtcctt gtagcggagc
     6241 ccgaacgcga cgttctccgc gaccgtgagg tgcgggaaca gggcgtagtt ctggaagacc
     6301 gtgttgacgt tgcgcttgtg cggcggcgtc tgcaccatgt cgacgccgtc gagcaggatc
     6361 tggccctcgt ccggccgttc gaacccggcg atgagccgaa gcgtggtcgt ctttccgcat
     6421 ccggaggggc cgagcagcga gaagaactcg cccgagggca tcgacaggtt gatccccgcg
     6481 accgccgtca cgtcggcgaa ccgcttcacc agatcgacga ggacgacctc tccaccggcc
     6541 atgaccgcgg agcatacccg a
//
LOCUS       DATBRK010000447         6901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1703468, whole genome shotgun sequence.
ACCESSION   DATBRK010000447 DATBRK010000000
VERSION     DATBRK010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703468"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2096)
                     /locus_tag="VIH70_13090"
     CDS             complement(<1..2096)
                     /locus_tag="VIH70_13090"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY8117344.1"
                     /translation="MRGVLAQESGPINAVFGSFETNALWVILVISLVALAFAYYLVRE
                     VLAAPEGSEKMREIARSIQIGAKAYLNRQFRTVAVFMALLTVLLFFALPVPENAAHSE
                     FALRFGRSVAFILGAAFSAITGYTGMWLAVRANVRTANAAKESGLRRALRIAFRAGGV
                     AGMFTVGLGLLGATVILLIYQQDATAVLVGFGFGGALLAMFMRVGGGIFTKAADVGAD
                     LVGKVEVGIPEDDPRNAATIADNVGDNVGDCAGMAADLFESYEVTLVASLILGAAAFA
                     GSEVGAIAGVMFPLFVRAVGVITSIIGILAVTPRSEDEHGMRAINRGFFLSAAISAAA
                     VFVISAIYMDDLRPAVAVLFGLILASVIQILTQYFTDTKFKPVQEIAESTQTGPATTI
                     LSGFSIGLESTVWAVLVIAAVISGAFVLGDNAREALYFISLTGMGMLTTVGVIVSMDT
                     YGPVSDNAQGIAEMSGEFEGRPAQVLTGLDAVGNSTKAITKGMAIATAVIAATSLFGS
                     FEEALRLAGFPFEGIRVDDPSVLVGLLIGGSIAFLFSSLAIRAVGRAASQVVVEVRNQ
                     FRDHPGIMTYEEKPDYARVVDICTRTSLRELMTPGLLAVLSPIIAGFALGAEALGAFL
                     AGSILTGQLLAVMLSNSGGAWDNAKKYIEEGHYGGKGSESHKAAVIGDTVGDPFKDTA
                     GPALNPLIKVMNLVALL"
     gene            2315..3718
                     /locus_tag="VIH70_13095"
     CDS             2315..3718
                     /locus_tag="VIH70_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8117345.1"
                     /translation="MGKRVVRSPRIGMLLFAFAFAVMADPVSSVAYAIEAALRALHGD
                     LGRLVLTMGLVLGIISLVRLNYDQLIRRFPEGGGAVAATGAAFGEGWAFLPLGALIVD
                     FVLTIAISVAAAASAVVAYFPSLAPYRIGIAVILCCFVAALTWFGHIGRSVFATMTVA
                     FVVAAIPVLLLGFLEPHAAGDAPFHGAGDASLLTVLLAFPVGMALATGVEAPSSAIAQ
                     LGQLDDRGRSRFGRITLWLTFAIEGSLTVAIAVLAVKLRAGVPRANSTLIADVARLSS
                     GDGALFAIFQLSSSLLLLAAAASSFQAGPGLLKALARRTRASGERVGILHQALGRTNA
                     HHTPYWSVVVYLVFAVVIVVAAGGREQELVLFYAVAVFVSFLSGLLAIAHFSRKEGSL
                     RPTIVNLFGAGVVAFTIVVNLRRGFPIASLIASALIGGVFAWLWIRQGRPRGIAQAER
                     EMEREFDPGGATDATPT"
     gene            complement(3793..3960)
                     /locus_tag="VIH70_13100"
     CDS             complement(3793..3960)
                     /locus_tag="VIH70_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117346.1"
                     /translation="MDWMVVIGLLDLSIYASVRLITWLEVKIGSLKLMHEVYRELGVR
                     RDRRIGGDWGS"
     gene            complement(4219..5580)
                     /gene="nhaA"
                     /locus_tag="VIH70_13105"
     CDS             complement(4219..5580)
                     /gene="nhaA"
                     /locus_tag="VIH70_13105"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY8117347.1"
                     /translation="MGSAMTDGTPRGAQSPTDVRPSWSRSRGPVARRIVQPLQSFLET
                     ETSSAILVLAAVGVALLWANGPYAGSYDRFWTTELVVRVGDRALSYDLRGWVSDGLMT
                     LFFLVVGLEIKRELLTGELRESRAALLPVAAALGGMLVPALLYLAFTAGTPAAPGFGI
                     AMPTDVVFALSVLALASPLPPGLKALLLTLAIVDDLASIVVVAFTYSRSVDVVPLSVA
                     LVVLAIYWLLWRIDVRAIVVYLGLGLVVWTALAQAGVSPTLAGVALAFLTPAVAFQRP
                     RAVSEEAHRVADHTVDDPVPPDADAAHWLYLATLSKEAVSPLARAEAVLLPWASYVVV
                     PLFVLAFAGIDLSRSTLIDAVTSRVGVAILVSRVVGKPLGIVLAGIAAMWVGAKLPPD
                     VRRRHLLGLGVVAGIPFTVSLYIAEVSLPVALIPTATASIIVAAAVAGALGSVVLSAR
                     ARH"
     gene            5696..6394
                     /locus_tag="VIH70_13110"
     CDS             5696..6394
                     /locus_tag="VIH70_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117348.1"
                     /translation="MTPNRAQRGANKKRRLPLALLIAPVVLAAIGVAIVLVLRGGADS
                     IIGGGDEPAPPFDFIVKPATAIPTAPEANEAALSASADEVAQEITPSIDDLYTKAFLD
                     PSNWRHDDYEEVFATFAPHAAATAEQSVETLTLGATAGDVFATVDPGKSKLSYQVLFD
                     PDGGPETAVVSVIFRATAERKAGTYLAIVSEGEFFLRQIDGQEDWTITAFDVTRDDHE
                     THPPSPSASTSPSA"
     gene            6391..>6901
                     /locus_tag="VIH70_13115"
     CDS             6391..>6901
                     /locus_tag="VIH70_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY8117349.1"
                     /translation="MSMPARRRAALLVALLAAWVAGSALGATHPAEHASAAPLFQLEP
                     AHAEYVPVLDGSKPIHVLVIGSDARPDQAVESQRADSLHIRSINPAEHRAAIVGIPRD
                     SYVEIPDHGQNKINTALFYGGPDLVVRTVEQLTGLKMDYWTMTGFATFEHMVDDVDGL
                     VVDVPFAMND"
BASE COUNT         1147 a         2430 c         2290 g         1034 t
ORIGIN      
        1 agcagcgcga ccaggttcat caccttgatg agcgggttga gcgccggccc ggccgtgtcc
       61 ttgaacggat cgccgaccgt gtcgccgatc accgcggcct tgtgcgactc gctgcccttg
      121 cccccgtagt ggccctcctc gatgtacttc ttggcgttgt cccacgcgcc gcccgagttc
      181 gagagcatca cggcgagcag ctggcccgtg aggatcgagc ccgcgaggaa ggcaccgagc
      241 gcctccgcgc cgagcgcgaa gccggcgatg atcggcgaca ggacggccag cagccccggc
      301 gtcatgagct cgcggagcga cgtcctggtg cagatgtcca cgacgcgcgc gtagtccggc
      361 ttttcctcgt aggtcatgat ccccgggtgg tcgcggaact ggttccgcac ctcgaccacg
      421 acctgggacg ccgctcgacc gaccgcgcgg atcgcgagcg acgagaatag gaaggcgatc
      481 gagccgccga tcaacagccc gacgagaacg ctcggatcgt ccacgcggat cccctcgaac
      541 gggaaccccg ccagacgcag cgcctcctcg aacgaaccga acagcgacgt cgcggcgatc
      601 accgcggtgg cgatcgccat gcccttggtg atggccttcg tcgagttccc gacggcgtcg
      661 aggccggtga gcacctgcgc cggacgaccc tcgaactcac ccgacatctc ggcgatcccc
      721 tgcgcgttgt ccgacaccgg cccgtaggtg tccatcgaga cgatcacgcc caccgtcgtg
      781 agcatcccca tcccggtgag cgagatgaag tagagggcct ccctggcgtt gtcgccgagc
      841 acgaacgcgc cggagatgac ggcggcgatc accaagacgg cccacacggt cgattcgagg
      901 ccgatcgaga agcccgacag gatcgtcgtg gccgggcccg tctgtgtcga ctccgcgatc
      961 tcctgcacgg gcttgaactt cgtgtccgtg aagtactgag tgaggatctg gatcacgctc
     1021 gcgaggatca ggccgaacag cacggcgacg gcgggccgca ggtcgtccat gtagatcgcg
     1081 ctgatcacga acaccgcggc ggccgagatc gcggccgaga ggaagaagcc acggttgatg
     1141 gcccgcatgc cgtgctcgtc ctcgctccgc ggcgtcacgg cgaggatccc gatgatcgac
     1201 gtgatcaccc cgacggcgcg cacgaacagc gggaacatca cgccggcgat cgcccccacc
     1261 tccgagccgg cgaacgcggc cgcgcccagg atcagcgacg cgaccagcgt cacctcgtag
     1321 gactcgaaca gatctgccgc catccccgcg cagtcgccga cgttgtcgcc cacgttgtcg
     1381 gcgatcgtcg cggcgttgcg cgggtcgtcc tcggggatcc cgacctcgac cttacccacg
     1441 aggtcggcgc ccacgtccgc cgccttggta aagatgccgc cacccacccg catgaacatc
     1501 gccagcagcg caccgccgaa cccgaacccg accaggacgg ccgtcgcatc ctgctggtag
     1561 atgagcagga tcacggtggc gcccaggagc ccgagcccga ccgtgaacat cccagccacc
     1621 ccgccggccc ggaacgcgat ccgcaacgct cgtcgcaggc ccgactcctt ggcggcgttc
     1681 gcggtgcgca cgttggcgcg gaccgcgagc cacatccccg tgtacccggt gatcgcgctg
     1741 aacgctgccc ccaggatgaa cgcgaccgag cggccgaacc gcagcgcgaa ctcgctgtgg
     1801 gcggcgttct cgggcaccgg gagcgcgaag aacagcagga ccgtgagcag ggccatgaag
     1861 accgcgacgg tgcggaactg ccggttcagg taggccttcg cgccgatctg gatcgaacgg
     1921 gcgatctccc gcatcttctc gctcccctcg ggagcggcca gcacctcgcg gaccaggtag
     1981 tacgcgaacg ccagggcgac gagcgagatg accaggatca cccacaacgc gttggtctcg
     2041 aacgacccga agacggcgtt gatcggaccg ctttcctggg ccaggacacc gcgcagcacg
     2101 tttcgacctc ctcgggtttc gaagatgcac cggtcccggc cgggccggga acccggcaag
     2161 tatagggagt cgcccgtcgg gcggctctcc aaggcaagcc ggggttgctc ccgattcccg
     2221 ggacccctac gatgcgaaac cgcagcacag gggtgccggg aagcctggtc ggcgtcgggt
     2281 cccggcgcct tcttgcgttc caggaggcag cgtggtgggc aagcgtgtcg ttcgttcgcc
     2341 gcgtatcggc atgttgctgt tcgcgttcgc gttcgccgtg atggccgatc ccgtctcatc
     2401 cgtggcgtac gcgatcgagg ccgctctccg agctcttcac ggcgatctcg gtcggctcgt
     2461 gctcacgatg ggcctcgtgc tcgggatcat ctcgctggtc aggctgaact acgatcagtt
     2521 gatccgcagg ttccccgagg gcgggggggc cgtcgccgca acaggggcgg cgttcggcga
     2581 ggggtgggcc ttcctgccat tgggagctct gatcgtcgac ttcgtgctga cgatcgccat
     2641 cagcgtggcg gccgcggcct ccgcggtcgt tgcctacttc ccctcgctcg ccccgtatcg
     2701 gatcgggatc gccgtcatcc tctgctgctt cgtcgccgct ctgacctggt tcggtcacat
     2761 cggcaggagc gtgttcgcca cgatgacggt ggcgttcgtg gtggccgcca tccccgttct
     2821 gctgctgggg ttcctcgagc cccatgccgc tggggatgcg cccttccatg gagcgggtga
     2881 cgcgtcgctg ttgacggtcc tgctcgcctt tcccgtcggc atggccttgg caaccggcgt
     2941 ggaggcaccg tcgtccgcca tcgcccaact cgggcagctc gacgaccggg ggcgatcgag
     3001 gttcgggcgc atcacgctct ggctcacctt cgcgatcgag ggaagcctca cggtcgcgat
     3061 cgccgtgctt gccgtgaagc ttcgggcggg ggtgccccgc gccaacagca ccttgatcgc
     3121 ggacgtggcg cggctctcgt caggcgacgg cgcactcttc gcgatctttc agctcagcag
     3181 ttccctgctg ctgctcgccg cagccgcttc ctccttccaa gcgggacccg gcctgctgaa
     3241 agcactcgcc cgtcgaacga gagcgagcgg ggagcgggtc gggatcctcc atcaagctct
     3301 cggacgtacg aacgctcacc acacgccgta ctggagcgtc gtcgtctacc tcgtgttcgc
     3361 cgtggtgatc gtcgtcgccg cgggtggccg cgaacaggag ctcgtgctct tctacgcggt
     3421 cgcggtgttc gtgtccttcc tgtccggcct gctggcgatc gcgcacttct ctaggaagga
     3481 gggatcgctc cggccaacca tcgtgaacct cttcggagcc ggggtggttg ccttcacgat
     3541 cgtcgtgaac cttcggcgcg ggttccccat cgcctccctc atcgcttcgg cgctgatcgg
     3601 cggtgtcttc gcttggttgt ggatccgcca aggccggccc cgggggatcg cccaagccga
     3661 acgagagatg gagagggagt tcgacccggg gggcgcaaca gacgccacgc cgacctgact
     3721 cggcggggtt cgtgatccgg ggacgaggcg atcgtcctcg atccgtggcc gctgctcgga
     3781 tcgtacgcgc ccctacgagc cccagtctcc cccgatccga cgatcgcgac gaacgccgag
     3841 ctcccggtag acctcgtgca tgagcttgag cgacccgatc ttcacctcca gccatgtgat
     3901 cagtcgcacg gaggcataga tcgacaggtc gagcaacccg atcacgacca tccagtccac
     3961 catgctcaac ggggaccggt ccgtcgctcg gggtgggatg cccacagcca cccagcgcac
     4021 gacttcgaga ctcgcgagga ggagaagagc catccgacct ccaaccgccg ataggtcgga
     4081 gaccggtcac gtggcgagtg tgccggtccc gccgaccagg cttcccggca caccatctcg
     4141 aagcctaacc acgggtcgaa ggaggtgcaa gggcggaggc gccggtgtcc tgcggggcgt
     4201 gctccggagc tccgcccctc agtggcgggc gcgtgccgag agaacgaccg atcccagcgc
     4261 accggcgacg gccgccgcca cgatgatgga agccgtcgca gtcgggatca gggcgaccgg
     4321 aagcgagacc tccgcgatgt agagcgagac cgtgaagggg atgccggcga cgacaccgag
     4381 accgagtagg tgccgacgtc ggacatcggg aggcagcttg gcgcccaccc acatcgccgc
     4441 gatgcccgcc aacacgatcc cgagaggctt ccccaccacc ctggagacaa ggatggcgac
     4501 cccaaccctg ctcgtcacgg cgtcgatgag cgtcgaccgg gagagatcga tcccggcgaa
     4561 ggccaggacg aacaggggga ccaccacgta gctcgcccag ggcagcagga cggcctcggc
     4621 ccgggcgagc ggggacacag cttccttcga gagggtcgcg aggtatagcc agtgtgctgc
     4681 gtcggcatcc ggcggaaccg ggtcgtcgac cgtgtggtcg gcaaccctgt gagcttcttc
     4741 gctcaccgct cgtgggcgct ggaacgccac cgcgggcgtc aggaaggcga gggccacgcc
     4801 ggcgagcgtc ggtgacaccc cggcctgcgc gagagcggtc caaacgacga ggccaagccc
     4861 gaggtagacc acgatggccc ggacatcgat ccgccagagc aaccagtaga tcgcgaggac
     4921 aaccagcgcg accgagagcg gcacgacgtc gacgctacgc gagtacgtga acgccaccac
     4981 gacgatgctg gcgaggtcat cgacgatcgc gagggtgagg agtaatgcct tcaaccccgg
     5041 cggcagtgga ctcgccaagg ccagcacgct gagcgcaaag acgacgtcgg tcggcatcgc
     5101 gatcccgaac cccggcgctg ccggggtgcc ggccgtgaac gcgaggtaga gcagcgcagg
     5161 gacgagcatg cccccgaggg cggcagcaac cggaagcagg gcggcgcgcg actcccgcag
     5221 ttcccctgtc aggagctcgc gcttgatctc gagccccacg accaggaaga agagtgtcat
     5281 caacccgtcc gagacccaac cccggaggtc gtaggagagc gcccgatcgc ccacccgcac
     5341 gacgagctcg gtggtccaga acctgtcgta cgacccggcg taaggcccat tggcccacag
     5401 gagggcgact ccgacggccg ccaggacgag gatcgccgag gaggtctccg tctccaggaa
     5461 cgattggagc ggctggacga ttcgccgtgc gacgggcccc ctcgaacgcg accagctcgg
     5521 tcgcacatcg gtcggcgact gggccccgcg gggagttcca tcggtcatgg ctgatcccat
     5581 gccgaccagt cttcccggcg caccttcaga acttctccat cctacttgtg ttgcgatgga
     5641 tcccgggcac gggtagcctt cacgcgtttt ggccaccagc accgaacccc cctccgtgac
     5701 ccccaaccgg gcgcagcgcg gcgcgaacaa gaagcgccgt ctgccgctcg cgttgctcat
     5761 cgcccccgtc gtgctcgctg cgatcggtgt ggcgatcgtg ctcgtgctgc ggggcggggc
     5821 ggactcgatc atcggcggag gcgacgagcc cgcgccgccg ttcgacttca tcgtcaagcc
     5881 cgccacggcg atcccgaccg cacccgaggc gaacgaggca gcgctgtcgg cgagcgccga
     5941 cgaggtggcg caggagatca cgccgtcgat cgacgacctg tacacgaagg ccttcctgga
     6001 cccgagcaac tggcgccacg atgactacga ggaggtcttc gcaacgttcg ctccgcatgc
     6061 ggccgcgacg gccgagcaga gcgtggagac cctcacgctg ggcgccaccg ccggcgatgt
     6121 cttcgccacc gtcgacccgg gcaagagcaa gctctcctac caggtgctgt tcgaccccga
     6181 cggcgggccg gagaccgccg tcgtgtccgt gatcttccgg gccacggcgg aacgcaaggc
     6241 cggcacgtat ctggcgatcg tgagcgaggg ggagttcttc ctccgtcaga tcgacggcca
     6301 ggaggattgg acgatcaccg cgttcgacgt gacgcgggac gaccacgaga cgcatccgcc
     6361 ctcgccctcg gcctcgacct ctccctccgc atgagcatgc ccgcccgccg tcgagccgcg
     6421 ctcctggtcg ccctcctggc ggcctgggtc gccggctccg ccctcggcgc cacgcatccg
     6481 gccgagcacg cgtcggcggc gccgctgttc cagctcgagc cggcccacgc cgagtacgtc
     6541 cccgtgctcg atgggagcaa gcccatccat gtcctggtca tcggctcgga cgcccggccc
     6601 gaccaggcgg tcgagagcca gcgcgcggac tcgctccaca tccggtccat caaccccgcc
     6661 gagcacaggg cggcgatcgt cgggatcccc cgcgactcct acgtcgagat ccccgaccac
     6721 gggcagaaca agatcaacac cgccctgttc tacggcggcc ccgacctggt cgtgcggacg
     6781 gtcgagcagc tcaccggcct gaagatggac tactggacga tgacggggtt cgcgaccttc
     6841 gagcacatgg tggacgacgt cgacgggctc gtggtcgacg tgccgttcgc gatgaacgac
     6901 t
//
LOCUS       DATBRK010000448         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2141061, whole genome shotgun sequence.
ACCESSION   DATBRK010000448 DATBRK010000000
VERSION     DATBRK010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141061"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VIH70_13120"
     CDS             <1..988
                     /locus_tag="VIH70_13120"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY8117350.1"
                     /translation="RAGPRGVLITLADGIKLFFKEGITPALADRPVYLLAPVIAMLPA
                     FLAFAVIPFGTGVTLLGREVPFQIADLSIGILWILAMSSLMVYAIVLAGWSSGSNYPL
                     LGGVRSSAQMISYEIGMALAIVAVVMYSDTLRMSEIVASQDRIWNVFPQFASFVIYLV
                     CGLAETNRPPFDLPEAETELVAGYHTEYSGIKFAMFYLGEYLNTVTVAAVATTLWLGG
                     WRGPAPDVVPWLWPLLWFLAKVLLIVYVYIWVRATLPRFRYDRLMAFGWKVLIPIGLV
                     WVMFTGAVVVLPEEFGRDTVVTALAIGAGVVIAITLIWPLVARRPPEDVSPS"
     gene            1090..1527
                     /gene="nuoI"
                     /locus_tag="VIH70_13125"
     CDS             1090..1527
                     /gene="nuoI"
                     /locus_tag="VIH70_13125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY8117351.1"
                     /translation="MGTVFKQIFRRDVTEQYPKEIVPLPARAHMGRHRLNRYDNGLEK
                     CIGCELCAFACPADAIYVQGAENDRDNPVSPGERYAADYQINYLRCIGCGLCVEACPV
                     RALTLTSYYEMAFQTREEAIFTKEQLLEPPPALGTAEADREGI"
     gene            1527..>1755
                     /locus_tag="VIH70_13130"
     CDS             1527..>1755
                     /locus_tag="VIH70_13130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY8117352.1"
                     /translation="MIGGIAPDSIVFWIIAPISVVSAGAMILARNAVHAALLLVVNFF
                     TLALFFLILGSPFLFVVQIIVYAGAIMVLFLF"
BASE COUNT          249 a          567 c          611 g          328 t
ORIGIN      
        1 gcgcgcgggt ccgcggggcg tgctgatcac gctcgccgac gggatcaagc tcttcttcaa
       61 ggaggggatc acgccggcgc tcgcggaccg gccggtctac ctgctcgcac cggtgatcgc
      121 gatgctgccc gcgttcctcg cgttcgcggt gatcccgttc gggaccggcg tcacgctgct
      181 cgggcgcgag gtgccgttcc agatcgctga tctgtcgatc ggcatcctgt ggatcctcgc
      241 gatgagctcg ctgatggtct acgcgatcgt gctggcgggc tggtcgagcg gatcgaacta
      301 cccgctgctc gggggcgtgc ggagctcggc ccagatgatc agctacgaga tcggcatggc
      361 gctggcgatc gtcgcggtcg tcatgtactc ggacacgctg cggatgtccg agatcgtcgc
      421 gagccaggac cggatctgga acgtgttccc gcagtttgct tcgttcgtga tctacctggt
      481 gtgcgggctc gccgagacca accgcccgcc gttcgacctg ccggaggccg agaccgagct
      541 cgtcgccggg taccacacgg agtactcggg gatcaagttc gcgatgttct acctcggcga
      601 gtacctgaac acggtcaccg tggccgcggt cgcgacgacg ctgtggctcg gcgggtggcg
      661 cgggccggcg cccgacgtcg tgccgtggct ctggccgctc ctgtggttcc tcgccaaggt
      721 cctgctcatc gtctacgtct acatctgggt ccgcgccacg ctcccgcggt tccggtacga
      781 ccgcctgatg gcgttcgggt ggaaggtcct gattccgatc ggtctcgtgt gggtgatgtt
      841 caccggcgcg gtcgtggtgc tgcccgagga gttcggacgc gacacggtcg tcacggcgct
      901 cgcgatcggc gcgggggtgg tcatcgcgat cacgttgatc tggcccctgg tcgcgaggcg
      961 accacctgag gatgtgtcgc cctcgtgagc gacgaaggca aggccacggg atcgaccacg
     1021 ggatcggcca agggatcgca gagatcgggg ccgacggtcg gttcgccgac gggggcgatc
     1081 gcccgaggga tgggaacggt cttcaagcag atcttccggc gcgacgtcac cgagcagtac
     1141 ccgaaggaga tcgtgccgct gccggcccgc gcacacatgg gtcgccaccg gctgaaccgc
     1201 tacgacaacg ggctcgagaa atgcatcggg tgcgagctgt gcgcgttcgc ctgcccggcc
     1261 gacgcgatct acgtccaggg agcggagaac gaccgggaca atccggtgtc gccgggggag
     1321 cggtacgcgg ccgactacca gatcaactac ctgcggtgca tcgggtgcgg gctatgcgtg
     1381 gaggcgtgtc cggtccgcgc tctcaccctg acgagctact acgagatggc gttccagacg
     1441 cgggaggagg ccatcttcac gaaggagcag ctgttggagc ctccgcccgc cctcggcacc
     1501 gccgaggccg accgggaagg catctgatga tcggcggcat cgcccccgac tccatcgtgt
     1561 tctggatcat cgccccgatc tcagtcgtgt cggcaggggc gatgatcctg gcccgaaacg
     1621 ccgtccacgc cgcgttgctc ctggtggtca atttcttcac gctggccctg ttcttcctga
     1681 tcctgggctc gcccttcctg ttcgtcgtcc agatcatcgt gtacgcgggc gcgatcatgg
     1741 tgctgttcct gttcg
//
LOCUS       DATBRK010000449         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_123674, whole genome shotgun sequence.
ACCESSION   DATBRK010000449 DATBRK010000000
VERSION     DATBRK010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123674"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VIH70_13135"
     CDS             complement(<1..835)
                     /locus_tag="VIH70_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8117353.1"
                     /translation="MRRIAVAAALLLLVAAVFTALGAAAPSGKVARTAAPLAKPKKPA
                     KPKLPPLPSAVKSRKHWEIGVKCDFPPFGFIDVRGNNDGYDVQVAQRFAQLAFGSKKK
                     VNFTCVTTPSRIPTLMSGRVDIIVSTLTWTQARADQIDFSIPYYSATGRLLVPNNSSA
                     TVATLSGKTVVTTRGALYAAWTRNCFKNTKLLEVDSPALAVSAVKDGRADTFMFDDAF
                     LLGVATQDRDVRLTNDKFLNVPWGIGIRKGETAMSRWVNAALRYMKKRDEFVKILRAN
                     AP"
     gene            complement(832..1605)
                     /locus_tag="VIH70_13140"
     CDS             complement(832..1605)
                     /locus_tag="VIH70_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY8117354.1"
                     /translation="MVRLEDVHKWFGNLHVLKGVDLNMQQRDVVVVIGPSGGGKSTLL
                     RCVSLLEVPTTGQVWFDNQNITDIRADLNEVRTHIGIVFQAYNLFPHMTALRNVTLAL
                     EKVLRLSSDEAHDRAMDQLARVGMGHKAGAFPGQLSGGQQQRVAIARALAMQPKVMLF
                     DEVTSALDPELVGEVLVVMRDLAHDGMTMLVVTHEMQFARDVGDHLIFMDDGKIVEQG
                     KPADVLDRPQAERTKRFLRRTLQLADSLEELTIDEGGIR"
     gene            complement(1650..2798)
                     /locus_tag="VIH70_13145"
     CDS             complement(1650..2798)
                     /locus_tag="VIH70_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8117355.1"
                     /translation="MVVREAPSIEPDVAPDVRGPTAPLPATIFALGIASLVVITLGMV
                     VSHVNYALSDRVLTQNCLNEQISPEDNGAIGVCNIVEALEDPLTTGSLYLGMGLGVLA
                     LVLGASTYRRMDSKRRRDQAITGAVLGSQAVLLAIAILWFRSATPGLFAKHFLNFADL
                     RGYGGAFVRGARNTLILAFAGEMGGIVIGLVLGILVLSQRRAVRAPARTYINFFRGTP
                     LIWQLVTGYFLLLFGFGIRMTAFVVAIIVFALNTGAYAAEVFRAGIQSIERGQIEAAR
                     SLGMSYFQAMRYAIVPQAVRRVIPPLMNEFVILIKDTALVVVLGLTANEYDIFTTARE
                     GQSATFNATFFTVAAIAYLIVTLPLIRLVNAAEKRLRSGLVGIAGAGG"
     gene            complement(2864..3736)
                     /locus_tag="VIH70_13150"
     CDS             complement(2864..3736)
                     /locus_tag="VIH70_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8117356.1"
                     /translation="MKRTRVWLAALIAVFALIVAACASNEDEGPTAATGTTAATGATG
                     ETGIPTFTTIEEGKLTVGSCLDYPPFESVKGGVETGFDVDITNEIASRLGLEVVWVKA
                     DFDTIFTAVAGDEFDMVAAASTITDEREQVVDFSVPYYNALQSLTVNTQETPDITSTD
                     QLGDGDVVGVQKGTTGKIWAEENLVPQGVELKTFTASPDSFRDLEAGNIIGVINDKTA
                     SVEIIKDLPSLAVVQDIETNEHYGLAFSPSNPDLREAVNTVFAQMVADGTYATIYGKY
                     FPPDSLPPEFAPSS"
     gene            complement(3841..>4393)
                     /locus_tag="VIH70_13155"
     CDS             complement(3841..>4393)
                     /locus_tag="VIH70_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8117357.1"
                     /translation="EALPGATARTVDPSAAAAVAGEAGCDVVIWAGGPTAGAEAARPL
                     QRLDVAFIGGDRLLDPDFLSTAGPAAEGAVALCACANVSTSTELAARRFIQDYQSEHG
                     VAPGAFAVEGWDAAHLILRAAGDRGITRALVARSLGGLTTADGLVGTRRFAADGEPLE
                     PRALLRLYRVEGGRWVRAGWDEI"
BASE COUNT          719 a         1485 c         1439 g          750 t
ORIGIN      
        1 cgggtgcgtt ggcccgcagg atcttgacga actcgtcccg ctttttcatg tagcgcagcg
       61 cggcgttcac ccaacgcgac atcgccgtct cgcctttgcg gatgccgata ccccacggca
      121 cgttgaggaa cttgtcgttc gtgagcctga cgtcgcggtc ctgcgttgcc acgccgagca
      181 ggaatgcatc gtcgaacatg aacgtgtcgg cacgcccgtc cttcaccgcc gagacggcga
      241 gagccggcga gtcgacctcg agcagcttcg tgttcttgaa gcagttcctc gtccaagccg
      301 catacagcgc tcctcgagtg gtcacgacgg tcttgccgga aagcgtcgcg accgtcgcgg
      361 aggagttgtt cggaacgagg aggcgtcccg tcgccgagta gtacgggata gagaagtcga
      421 tctggtcggc acgcgcctgc gtccaggtca gcgtcgagac gatgatgtcg acgcggcccg
      481 acatcaaggt cgggatgcgg ctcggcgttg tcacgcaggt gaagttcacc ttcttcttgc
      541 tgccgaacgc cagctgcgcg aagcgttgcg ccacctgcac gtcgtagccg tcgttgttcc
      601 cgcggacgtc gatgaagccg aaaggcggga aatcacattt gacgccgatc tcccaatgct
      661 tgcggctctt gacggccgac ggcagcggcg gcagcttcgg tttggcgggc tttttcggtt
      721 tcgcgagcgg tgcggctgtc cgggcgactt tcccgctggg cgcggccgct ccgagcgccg
      781 tgaatacagc cgcgaccagc agcagcaggg ctgcggcgac tgcgatacgc ctcatctaat
      841 ccctccttcg tcgatggtga gctcctccaa ggagtccgcg agctggagcg tccggcggag
      901 gaatcgtttc gtgcgttccg cctgcggccg gtcgaggacg tcagccggct tgccctgctc
      961 gacgatcttg ccgtcgtcca tgaagatcag atgatcgccg acgtcgcgtg cgaactgcat
     1021 ctcgtgcgtg acgacgagca tcgtcattcc gtcgtgcgcg aggtcgcgca tgacgacgag
     1081 gacttcgccg acgagctccg gatcgagcgc cgaggtgacc tcgtcgaaca gcatcacctt
     1141 cggctgcatg gcgagggccc gggcgatcgc gactcgttgc tgctgccccc ccgacagttg
     1201 cccggggaag gcgccggcct tgtggcccat ccccaccctg gcgagctgat ccatcgctcg
     1261 atcgtgggcc tcatccgatg aaagcctgag gaccttctcc agcgccagcg tgacgttgcg
     1321 cagggccgtc atgtgcggga acaggttgta cgcctgaaag acgatgccga tgtgtgtcct
     1381 aacctcgttc aggtcggctc gaatgtcggt gatgttctgg ttgtcgaacc acacctgccc
     1441 cgtcgtcggg acctcgagca agctcacgca gcggagcaga gtcgacttcc ctcccccgga
     1501 gggcccgatg acgaccacga catcgcgctg ctgcatgttg agatccacgc ccttgaggac
     1561 atgcaggttc ccgaaccact tgtggacgtc ctccagccgg accagcggct cccctacgct
     1621 ctgggcgcga accgatcctg ccggttcgct cacccgcccg ccccggcgat cccgacgagt
     1681 ccgctccgca ggcgcttctc ggccgcgttg accaggcgga tgagcgggag cgtgacgatc
     1741 aggtaggcga tcgcggccac ggtgaagaag gtggcgttga acgtggccga ctggccctcc
     1801 cgggcggtcg tgaagatgtc gtactcgttc gcggtcagcc cgaggaccac caccagcgcc
     1861 gtgtctttga tcaggatcac gaactcgttc atcagcggcg ggatcacacg acggaccgcc
     1921 tgcgggacga tcgcgtaccg catcgcctgg aagtagctca tcccgagcga gcgggcggcc
     1981 tcgatctgcc cgcgttcgat cgactggatc ccggcacgga acacctcggc agcgtacgcg
     2041 ccggtgttca gcgcgaacac gatgatggct accacgaacg ccgtcatacg gatgccgaac
     2101 ccgaagagga gcaggaagta cccggtgacc agctgccaga tcagtggcgt cccacgaaag
     2161 aagttgatgt aggtccgtgc cggggcccgg accgcccgcc gctgggagag cacgaggatc
     2221 ccgaggacga ggccgatcac gatgccgccc atctcgccag cgaacgcgag gatcaaggtg
     2281 ttccgtgcgc cccggacgaa ggctcccccg tatccccgga gatccgcgaa gttgaggaag
     2341 tgtttcgcga acaacccggg ggtcgccgac cggaaccata agatggcgat cgccaggagc
     2401 acagcctgcg acccgagcac cgctccggtg atcgcctgat cgcgccgacg cttgctgtcc
     2461 atcctgcggt aggtcgaggc cccgagcacc agcgcgagaa caccgaggcc catccccagg
     2521 tagagcgatc ccgtggtgag ggggtcctcg agcgcctcga cgatgttgca gacaccgatc
     2581 gccccgttgt cctcgggcga gatctgctcg ttgaggcagt tctgcgtcag gacccggtca
     2641 gagagcgcgt agttcacgtg cgagacgacc atgccgagcg tgatcaccac gagggaggcg
     2701 atgccgagcg cgaagatcgt ggccggcagc ggggccgtgg gacctcgaac gtccggggcg
     2761 acatcgggct cgatcgatgg agcttcccgt acgaccatgc gtcacctccg tgatgcaagg
     2821 gcccgcgcta tcccgcgcgg gcccttggcg atcgaacggt ccgtcaggaa ctcggtgcga
     2881 actccggtgg cagagagtcg ggcgggaagt acttaccgta gatcgtggcg tacgttccgt
     2941 ccgcgaccat ctgcgcgaac actgtattca cggcctcccg caggtcggga ttcgagggcg
     3001 agaacgcgag cccgtagtgc tcgttggtct cgatgtcctg cacgaccgcc agggacggca
     3061 ggtccttgat gatctcgacg gaagccgtct tgtcgttgat gaccccgatg atgttgccgg
     3121 cctcgaggtc gcggaacgag tcgggcgaag ccgtgaaggt cttgagctcg acgccctggg
     3181 gtacgaggtt ctcctctgcc cagatcttgc ccgtcgtgcc cttctgaacc ccgacgacgt
     3241 cgccgtcgcc cagctggtcg gtggaggtga tgtcgggcgt ctcctgcgtg ttcaccgtca
     3301 gggactgcag cgcgttgtag tacggaaccg agaagtccac gacttgttcg cgctcgtcgg
     3361 tgatcgtcga ggccgccgcc accatgtcga actcgtcccc ggcgaccgcc gtgaagatcg
     3421 tgtcgaagtc ggcctttacc catacgacct cgaggccgag ccggctggcg atctcgttgg
     3481 tgatgtccac gtcgaacccg gtctcgactc cccccttgac cgattcgaac ggcgggtagt
     3541 ccaggcacga gccaacggtg agcttgccct cctcgatcgt cgtgaacgtg gggattccgg
     3601 tctcccccgt cgcgcccgtc gccgcagtcg tgcccgtggc ggcggtcggg ccctcgtcct
     3661 cgttgctcgc gcaggcagcc acgatcagcg cgaacaccgc gatcaacgct gccagccaga
     3721 ctctcgtccg cttcactgcg tccccctcct cgtgctctgg tcgggctcga aggtgcgaag
     3781 cacctatcct cccccggatc gggtcaatcg ctcaacttcc tgcccacgca tcggttcccg
     3841 tcatatctcg tcccaacctg cccggaccca tcgcccgccc tcgacccgat acaggcggag
     3901 caacgccctg ggttcgagcg gctcgccgtc ggcggcgaac cggcgcgtcc ccacgagacc
     3961 gtcggccgtc gtgagcccgc ccagcgagcg agccaccagg gcacgtgtga tcccccgatc
     4021 gcccgcagcc cgcaggatga ggtgggcggc gtcccagccc tcgaccgcaa aggccccggg
     4081 cgcgacgccg tgctcggact ggtagtcctg gatgaatcgc cgtgcggcga gctcggtcga
     4141 cgtggacacg ttcgcgcagg cgcagagcgc gaccgctccc tcggccgcgg gtccggcggt
     4201 cgacaggaag tcgggatcga ggaggcgatc accgccgatg aacgcgacgt cgagccgctg
     4261 gagggggcga gcggcctccg cacctgcggt cggccctccg gcccagatca cgacgtcgca
     4321 tcccgcctcg ccggccaccg ccgccgcggc cgagggatcg acggtccgtg ccgtcgcacc
     4381 gggcagggcc tcc
//
LOCUS       DATBRK010000450         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1564296, whole genome shotgun sequence.
ACCESSION   DATBRK010000450 DATBRK010000000
VERSION     DATBRK010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564296"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..848)
                     /locus_tag="VIH70_13160"
     CDS             complement(66..848)
                     /locus_tag="VIH70_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8117358.1"
                     /translation="MFTVEQRDALRERVLRLAQEDERVVAGAAVGSLALDGGDRFSDL
                     DLTFGVADHVRVADVLADWTRTLIHELAAVQLAELERGPTTYRVFLLPDLLQLDLSMT
                     PAAQFRPAGPRFRLLFGETAAGGSEAPAPSVGGDLFLSTPAVAADVFGWGVIYAVYAR
                     TCIERGRVWQAEHYVGAVRDHALSLACLRQGLPAVQARGYDDLSAETLTRFQDAHTGT
                     VEPGALRSALAGSVLALLREGSEARLLHADVIAERLAELGKP"
     gene            complement(896..1225)
                     /locus_tag="VIH70_13165"
     CDS             complement(896..1225)
                     /locus_tag="VIH70_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117359.1"
                     /translation="MDFEEALVKHGFHPASGRAPRGVQLLVAEPNPFLTYTVQAFDDG
                     TALFSWEFAVGEYLRTKGIQFGSDETLNQFMFPREDTRGPQEGAWLVEAIERARTQLA
                     SIRFDQP"
     gene            complement(1246..>1894)
                     /gene="rmuC"
                     /locus_tag="VIH70_13170"
     CDS             complement(1246..>1894)
                     /gene="rmuC"
                     /locus_tag="VIH70_13170"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY8117360.1"
                     /translation="REHLAQLPPGMLVTDLRVNGRVVEFGLLLPDGRRLPVDSKWSAV
                     AELEALEAADDPIERETLAREVERQVALRAREVAQYLDPSLTAPVAVAAVPDAAYEVL
                     RRAHADAFARGVVIVPYSTALPVLLFLYSLAHRFGDADDARSALEEVVSVLDAMQLVL
                     ENKVARAATMVSNGVDELRSGLGKARGSIARGRTAAAIEPEPELALVDEVLKAVP"
BASE COUNT          314 a          664 c          665 g          251 t
ORIGIN      
        1 ctacctggga ggacccgtga tgggcaacgc tcttggccga cccgcatgat gcgcgtaacc
       61 aagggtcatg gctttccaag ctcagcgagc cgctctgcga tgacatcggc atgcaggaga
      121 cgcgcctccg agccctcgcg cagaagcgcg agaacggagc cggcgagagc tgaccggagc
      181 gctccaggtt cgaccgtacc cgtgtgggcg tcctggaatc gagtaagggt ttcagcggaa
      241 aggtcgtcgt agccgcgcgc ctgcaccgcc ggcagcccct ggcggaggca cgcaagcgag
      301 agcgcgtggt cgcgcacggc accgacgtaa tgctcggctt gccagacacg cccgcgctcg
      361 atgcacgtgc gcgcatagac ggcgtagatg acgccccacc cgaagacgtc cgctgcgacc
      421 gccggcgtgg aaaggaagag gtcccctccc accgacggcg cgggagcctc ggacccgccg
      481 gcggccgttt cgccgaacag cagcctgaat cgtgggccgg cgggacggaa ctgggccgcc
      541 ggcgtcatcg acagatccaa ctgcaacagg tccggcagca ggaacacccg gtaggtggtc
      601 ggtccccgct cgagctcggc gagctgcacc gcggcgagtt cgtggatgag cgtgcgcgtc
      661 cagtcggcga gcacgtcagc gacccgaacg tggtcggcga cgccaaaggt gaggtccaga
      721 tcggagaagc ggtcgccgcc gtctagggcg agtgagccga cggcggcccc ggcgacgacg
      781 cgctcatcct cttgcgcgag cctgagcacg cgctcgcgga gagcatcccg ctgctcaacg
      841 gtgaacacag gcggcgatcc tagctttcca taggcggcgc gaagagacct ggcttctagg
      901 gctgatcgaa ccggatcgac gcgagctgcg ttcgcgcgcg ctcgatcgct tcgaccagcc
      961 aggcgccctc ctgcggcccg cgggtgtcct cgcgcgggaa catgaactgg ttgagggtct
     1021 cgtccgaccc gaactggatc cccttggtcc gcaggtactc gcccaccgcg aactcccagg
     1081 agaacagcgc cgtcccgtcg tcgaacgcct gcaccgtgta ggtcaggaac gggttcggct
     1141 cggcgacgag cagctggacc cctcgcggcg cacgtccgga agcgggatgg aacccgtgct
     1201 tcacgagcgc ttcctcgaaa tccaccgcgc gagcgtaccg cgccactagg gaacggcctt
     1261 cagcacctcg tcgacgagcg cgagctcggg ttcgggttcg atcgccgccg ccgttcggcc
     1321 gcgggcgatc gacccgcggg ccttgccgag acccgagcgg agctcgtcga cgccgttcga
     1381 gaccatggtg gcggcacgag cgaccttgtt ctcgaggacc agctgcatcg cgtcgagcac
     1441 cgacaccacc tcctcgagcg ccgaccgcgc gtcgtccgcg tcgccgaacc gatgcgcgag
     1501 gctgtagagg aacaggagca ccggcagcgc cgtcgagtac ggcacgatca ccacgccgcg
     1561 cgcgaacgcg tccgcgtgcg cacgccgcag gacctcgtac gccgcgtcgg ggacggcggc
     1621 gaccgcgacc ggtgcggtga gggacggatc gaggtactgc gccacctcgc gcgcccggag
     1681 cgcgacctga cgttcgacct cgcgtgcgag cgtctcgcgc tcgatcgggt cgtccgctgc
     1741 ctcgagcgcc tcgagctcgg ccaccgcgga ccacttggag tcgacgggca gccgtcggcc
     1801 gtcgggcagc agcagcccga actcgaccac gcggccgttc acgcgcaggt cggtgacgag
     1861 catgcccggc gggagctgcg cgaggtgctc gcgg
//
LOCUS       DATBRK010000451         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1706560, whole genome shotgun sequence.
ACCESSION   DATBRK010000451 DATBRK010000000
VERSION     DATBRK010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706560"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VIH70_13175"
     CDS             complement(<1..310)
                     /locus_tag="VIH70_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY8117361.1"
                     /translation="MDVLLPRVLDEALEMAAANPDVVPIAGGTDVMVELNMDLGRPET
                     LMDISRLPELQEVRREIGHLFLGAGVTYTRIMRELPQHTALVQASRSVGSPQIRNRGT
                     V"
     gene            complement(343..1098)
                     /gene="truA"
                     /locus_tag="VIH70_13180"
     CDS             complement(343..1098)
                     /gene="truA"
                     /locus_tag="VIH70_13180"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY8117362.1"
                     /translation="MIVRLGLAYDGTQFRGWARNRGQRSVEGVLSEALGLVLGDQPKI
                     SVAGRTDAGVHARGQVASFVAADDVDLERVQRSINGILAPEIVAWDARRAPEGFDARF
                     SASGREYRYRLDTGAWPDPFEARFVWHRPGSLAVASMREAARPLVGEHDFASFCRRPQ
                     SGGTVRRLERLSIARAGARVEISARANAFLHQMVRALVGTLVAVGDGGLDPGSVLEIL
                     RARDRSRAPQMAPAHGLTLERVIYGRRGPGRLI"
     gene            complement(1124..1471)
                     /gene="rplQ"
                     /locus_tag="VIH70_13185"
                     /pseudo
     CDS             complement(1124..1471)
                     /gene="rplQ"
                     /locus_tag="VIH70_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(1480..2403)
                     /locus_tag="VIH70_13190"
                     /pseudo
     CDS             complement(1480..2403)
                     /locus_tag="VIH70_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
     gene            complement(2487..2879)
                     /gene="rpsK"
                     /locus_tag="VIH70_13195"
     CDS             complement(2487..2879)
                     /gene="rpsK"
                     /locus_tag="VIH70_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY8117363.1"
                     /translation="MAKPKAKKTKRREKKNVLHGQAHIKSTFNNTIVTITDLQGNTIS
                     WASAGNVGFKGSRKSTPFAAQLAAESAARKAQEHGLQKVDVFVKGPGSGRETAIRSLA
                     ATGLEVAGIQDVTPVPHNGVRPKKRRRV"
     gene            complement(2898..>3166)
                     /gene="rpsM"
                     /locus_tag="VIH70_13200"
     CDS             complement(2898..>3166)
                     /gene="rpsM"
                     /locus_tag="VIH70_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY8117364.1"
                     /translation="DAGTKVRDLSEEEVVRIREFLDRNYKVEGDLRRDVAQDIKRKIE
                     IGCYQGIRHRRSLPVRGQRTHTNARTRKGPKKAIAGKKKLKSKK"
BASE COUNT          457 a         1100 c         1066 g          543 t
ORIGIN      
        1 ctacggttcc ccggttgcgg atctgcggcg accccaccga acgcgaggcc tggacgagcg
       61 ccgtgtgctg gggcagctcg cgcatgatcc gggtgtaggt cacgccagcc ccgaggaaga
      121 gatggccgat ctcgcggcgg acctcctgca gttcggggag gcgggagatg tccatgaggg
      181 tctcgggtcg gccgagatcc atgttgagct cgaccatcac gtcggtcccc ccggcgatcg
      241 gcacgacgtc ggggttcgcg gcggccatct cgagcgcctc gtcgagcacc cgaggaagga
      301 ggacgtccat accgagatgg tcggcggacg aaccgtcgtg ggtcaaatca ggcgcccggg
      361 accccttcgg ccatagatca cacgttcgag cgtcaggccg tgcgccggcg ccatctgagg
      421 ggctcggctg cggtcgcgcg ccctcaggat ctccagcacg gagcccgggt cgaggccgcc
      481 gtcccccacc gcgacgagcg taccgacgag agcccgcacc atctggtgga ggaatgcgtt
      541 cgcccgcgcc gagatctcga cgcgggcgcc cgccctcgcg atcgacaaac gctcgagccg
      601 ccgaaccgtg ccgcccgact gaggccgccg gcagaacgac gcgaaatcgt gctcgccgac
      661 cagcggacgc gctgcctccc gcatcgatgc gacggcgagc gatcccggcc ggtgccagac
      721 gaaccgagcc tcgaacggat ccggccacgc ccccgtatcg agccggtacc ggtactcgcg
      781 tcccgaggcc gagaacctag cgtcgaaccc ctcgggcgcc cgccgagcgt cccaagcgac
      841 gatctcgggc gcaaggatgc cgttgatcga ccgctggacg cgttcgagat cgacgtcgtc
      901 ggccgcgacg aaggacgcca cctgtcctcg agcgtgcaca ccggcgtcgg ttcgccccgc
      961 gacggagatc ttcggctggt ctccgaggac taggccgagc gcttcggaaa gcacgccctc
     1021 caccgatcgt tgccctcggt tcctcgccca gccacggaac tgggtgccgt cgtacgcgag
     1081 cccgagccga acgatcacgg tccgatggcc gctactcgga cccgacgagc tcgatccgag
     1141 ccatcggggc gccgtccccc tggcgctggc cgagcttgag gatccgggtg tagccgccgt
     1201 tgcgctcggc gaagcgcggg ccgatatcgc tgaacagctt gtggacgata tcgcggtccg
     1261 ggatctgttt gaggacctcg cggcgcgccg ccacgtcgcc cttcttggcg aacgtgatca
     1321 gcttctcggc caccgggcgc acggccttgg ccttcgcctc ggtcgtgttg atgtgctcgt
     1381 ggacgaacag ctgggcggcg atgccgttca gcatcagacg ctggtgggcg gggccggacc
     1441 ccaggcgggg acccttcttg ggcctgggca tccctactcc tcccgcagcc ccaggccgag
     1501 ctcctcgagc ttggccttga cctcgtcgat cgacttctgg ccgaagttgc ggatgtccat
     1561 cagctcggcc tcggacttct cgacgaggtc gccgaccgtg ttgatgccct cacgcttgag
     1621 gcagttgtac gagcgcaccg acaggttgag ctcctcgacc gtgatcgcgt agtccgaggg
     1681 caccgagtcg tcctcggcgg gaccgacctg catcgactgc gcctccgcca ggtcgccgaa
     1741 gagctggacg agctcgagca gggttccgcc cgccgaggcc agcgcctcgc gcggcgtgat
     1801 cgagccgtcg gtctcgacgt ccaggatcag ccggtcgcgg tcggtcatct gctcgacgcg
     1861 ggtgttctcg accgcgtacg agacgcggcg caccggggag aagatcgagt ccaccgggat
     1921 cacgccgatc gggtcgcgcg cgttcttgtt cttgtcggcc atccggtagc cgaccccgcg
     1981 ctcgacgttc atctccatct cgagcgaccc gcccttgccg agcgtggcca cgtgcaggtc
     2041 ggggttcaga acctggacgc cggcgggcgg cgcgatgtct ccggccgtga cggcgccggg
     2101 gcccttggcc ttcaggtaaa ccgtgacggg ctcctcggac tcagagcgga ccacgagctc
     2161 cttcaggttc atgatgatgt cggtgacgtc ctcggtgacc ttggggatcg tcgagaactc
     2221 gtgcagcacg ccctcgatcc gaaccgagct gatcgcggcc cccgggatcg aggacaacag
     2281 ggtgcgacgg agcgagttcc ccacggtgta gccgaacccg ggctcgagcg gctcgatgac
     2341 gaacttcgag cgggtaggcg agagggactc ctcctggatc tgcggtctcg cgacgatgaa
     2401 cacttctgct cctccttcga cgggggatca tccactcctc cccgccgcgt atgacgcccg
     2461 cttcgacgtg cgggcctgcc tgcgcgctag acgcgccggc gcttcttggg ccggaccccg
     2521 ttgtggggca ccggcgtgac gtcctggatc cccgcgacct ccaggccggt ggccgcgagc
     2581 gagcggatcg cggtctcgcg accggaaccg ggacccttca cgaacacgtc gaccttctgg
     2641 agcccgtgct cctgcgcctt acgggcggcg gactccgccg cgagctgggc ggcgaacggg
     2701 gtggacttgc gggagccctt gaacccgacg ttgccggccg aagcccacga gatcgtgttg
     2761 ccctgaaggt cggtgatcgt gacgatcgtg ttgttgaacg tcgacttgat gtgcgcctga
     2821 ccgtgcagca cgttcttctt ctcgcggcgc ttggtcttct tggctttggg cttcgccata
     2881 cgtcgagtgc tcccccgtta cttcttcgac ttgagcttct tcttgccggc gatggccttc
     2941 ttcgggccct tgcgcgtgcg cgcgttcgtg tgcgtccgct ggccccggac cggaaggctg
     3001 cggcgatggc ggatgccctg gtagcacccg atctcgatct tgcgcttgat gtcctgggcc
     3061 acgtcgcggc gcaggtcacc ctcgactttg tagttccggt cgaggaactc gcggatccgc
     3121 acgacctcct cctcggaaag gtcccggacc ttcgtccctg cgtcga
//
LOCUS       DATBRK010000452         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2144661, whole genome shotgun sequence.
ACCESSION   DATBRK010000452 DATBRK010000000
VERSION     DATBRK010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144661"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VIH70_13205"
     CDS             <1..565
                     /locus_tag="VIH70_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8117365.1"
                     /translation="GVGAVLNTAKVRPGDSVLVIGCGGVGLSAIQGARIAGASPIVAV
                     DVVEPKLEIAKELGATETVLAGRDPVPDRFDHALECLGRPETIEQAMRAVAPGGTAVL
                     VGMAAPGARALLDALRTTTKELTVVGSWYGSVVPPRDLPLLAHLLTFGELRLEPMIAR
                     TIALDGIHDALARFEAGDEARSVIVYD"
     gene            complement(556..1371)
                     /locus_tag="VIH70_13210"
     CDS             complement(556..1371)
                     /locus_tag="VIH70_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8117366.1"
                     /translation="MSEAYRSPLAPDANEDLVALVTGGGTGLGRAIAREYARTGARIV
                     IAGRRAEPLEEVASELAAAGAQVLSVPGTDVREPAQVASLVDTVLDRFGTIDVLVNNA
                     GGQFVATPEQISAKGWRAVHRLNVDAVWDLTRTVAELSMIPRGGGLVVFIGFSPRRGI
                     PLMVHASAARAALENLASGLAIEWSRHRIRAVCVATGAIDTDAFRAYGDEFVAEAMRT
                     APLGRIASPEEVASVIAFLSTTGGAYVTGTTIVVDGGNDAWGLGFPPPQPPQS"
     gene            1453..>1713
                     /locus_tag="VIH70_13215"
     CDS             1453..>1713
                     /locus_tag="VIH70_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY8117367.1"
                     /translation="MSSRLDELVRATPATRDRYVDFLRAASIVAVVFGHWFISINHLE
                     RDIYSTTSAVGLTSWMWLGTWLFQVMPVFFFVGGFSNLVTYDA"
BASE COUNT          228 a          617 c          610 g          258 t
ORIGIN      
        1 gggcgtcggc gcggtgctga acacggcgaa ggtgcgcccg ggtgactcgg tcctcgtgat
       61 cgggtgcggg ggcgtcgggc tctcggcgat ccagggtgcg cggatcgcgg gtgcctcgcc
      121 gatcgtcgcg gtcgacgtcg tcgagccgaa gctcgagatc gccaaggagc tcggcgcgac
      181 cgagacggtg ctcgcgggcc gtgaccccgt cccggacagg ttcgaccacg ccctggagtg
      241 cctcgggcgc cccgagacga tcgaacaggc gatgcgcgcc gttgcacccg gcgggacggc
      301 cgtcctggtc gggatggccg cgcccggcgc tcgcgcgctg ctcgacgcgc tccgcaccac
      361 gaccaaggag ctcacggtcg tcggttcgtg gtacggcagc gtggtgccgc cgcgcgacct
      421 gccgctgctc gcgcacctgc tgacgttcgg cgagctgcga ctcgaaccga tgatcgcccg
      481 gacgatcgcg ctcgacggca tccatgacgc gctggcccgg ttcgaggccg gggacgaggc
      541 gcgttcggtg atcgtctacg actgaggggg ttgcggcggc gggaagccga gcccccaggc
      601 gtcgttgccg ccgtcgacaa cgatcgtggt tccggtgacg tacgcgccac cggtggtcga
      661 caggaacgcg atcacgctgg ccacctcctc gggcgacgcg atgcgaccga gcggcgccgt
      721 gcgcatcgcc tccgcgacga actcgtcgcc gtaggcgcgg aacgcgtcgg tgtcgatcgc
      781 ccccgtcgcg acgcacaccg cgcggatccg gtggcggctc cactcgatcg cgagcccgct
      841 cgccaggttc tccagcgccg cccgcgcggc ggacgcgtgc accatcaacg ggatgcctcg
      901 gcgggggctg aacccgatga acaccacgag gccgccgcct ctgggaatca tcgagagttc
      961 ggcgacggtc cgggtgaggt cccagaccgc gtcgacgttg aggcggtgca ccgcccgcca
     1021 gcccttggcc gagatctgtt cgggcgtcgc gacgaattgc ccgcccgcgt tgttcacgag
     1081 gacgtcgatc gtcccgaacc ggtccaggac ggtgtcgacg agcgaggcca cctgcgcggg
     1141 ttcgcggacg tcggtgcccg gcaccgaaag gacctgagcg ccggcggccg cgagctcgga
     1201 ggcgacctcc tcgagcggtt cggctcgcct gccggcgatc acgatgcggg cgccggtccg
     1261 agcgtactcg cgcgcgatcg cgcggcccag ccccgtcccg ccgccggtca cgagcgccac
     1321 gagatcctcg ttcgcgtcgg gcgcgagcgg cgaacggtac gcctcggaca tgcgcgctca
     1381 tgatgccaga cccccggagc tcgaacccct ccttccttga cccggcgacc gccggggggt
     1441 agcacctggc tcgtgagctc acggctggac gagctggtgc gggccacgcc cgccacccgc
     1501 gatcgctacg tcgacttctt gcgtgcggcc tcgatcgtcg cggtcgtgtt cggccactgg
     1561 ttcatctcga tcaaccacct cgaacgcgac atctactcga cgaccagcgc cgtcggcctg
     1621 acctcctgga tgtggctcgg cacgtggctg ttccaggtga tgcccgtctt cttcttcgtg
     1681 ggtggcttct ccaacctcgt gacctacgac gcg
//
LOCUS       DATBRK010000453         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1126019, whole genome shotgun sequence.
ACCESSION   DATBRK010000453 DATBRK010000000
VERSION     DATBRK010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126019"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="VIH70_13220"
     CDS             <1..1485
                     /locus_tag="VIH70_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY8117368.1"
                     /translation="RQITHLTGIDDLVVAGAPPIEWVLPTFAEFARGCVFVAHNARFD
                     FTFLNVALERLDYDALPPPPVCTARLARRVVWPDVPNVRLHTLAQYFRTAVRPSHRAL
                     PDAEACSEVLQGLLELGGRLGIVTLGDLHGAVRARGRPNFGKIRLADHLPNAPGVYLF
                     RGRDGRVLYVGKSVDLRSRVKSYFYGDGRKKVEDLLAETASVDGVACGSELESLVLEA
                     RLIREHEPKYNRRGKTWRKYAYLRIDDAEAYPRIKVVRETKGGGAFLGPFPSSHLARL
                     AKEALEDAFPIRRCTTAMRASTRFAPCALAEMHRCLTPCDGRVGPERYEELVRSLISS
                     LSRPGGLLGTLEARMRDLAEQERFEEAALARDRLRALAQSLARARVDGWLLGAGHLVL
                     RDGQGRLFDVRRGALIRTERDEPLGSPCPRDRADELSVLRSWMSRHDVRIEAADVPIA
                     EPVDGGAVLHRILRTLRGATGEDGGGLRSLRDDRRARAAPARDG"
     gene            1445..1969
                     /locus_tag="VIH70_13225"
     CDS             1445..1969
                     /locus_tag="VIH70_13225"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8117369.1"
                     /translation="MTGGPELLLRETDDAYEILLRCIGDLTDEEYAWEPGPDAWRVFR
                     GEDGRWTHDYAEPDPEPSPFTTIGWRVAHVATCKVMYHEWAFGPQRLTWLTIETPHDV
                     EGALAMLREGHQLLRADLAALDEPGLNAEVLTNWGEPWPAWRIFWTMIHHDLQHGGEI
                     GALRDYLRTSPGTR"
     gene            2005..>3045
                     /locus_tag="VIH70_13230"
     CDS             2005..>3045
                     /locus_tag="VIH70_13230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY8117370.1"
                     /translation="METAVILEGARTPIGRFLGSFSELPAVDLGALAAREAMRRSGVE
                     PDQIDQTILGHARQAGNGPNTGRQVSVRAGVPVEVSAYNVNIACGSGMKAVQLGAQQI
                     ALGDSQVVLAGGMENMTRVPFLLERMRSGYRMGDERVYDAMYRDGLLDPLCGLIMGET
                     AENLVDRYGLSRAEQDEFALESQQKAAAAARARAAEMFAVEAPSGGGSVTVTEDEHPR
                     PDTTLERLAELEPVFREGGSVTAGNSSGITDGAAAMVLMSASQAEAEGRHPLARIVGM
                     AWAGVPPEIMGIGPVPATKRVLENTGLSLADIDLIEINEAFAAQVLACERELKFDRDR
                     LNVHGGGISLGHP"
BASE COUNT          420 a         1086 c         1120 g          419 t
ORIGIN      
        1 cgccagatca cgcacctgac cgggatcgac gacctggtgg tggccggcgc gcccccgatc
       61 gagtgggtgc tgccgacgtt cgccgagttc gcgcgcgggt gcgtcttcgt ggcccacaac
      121 gcccggttcg acttcacgtt cctcaacgtc gccctggaac gcctcgacta cgacgcgctc
      181 ccgccgccgc ccgtctgcac cgcccggctt gcgcgacgcg tggtgtggcc ggacgtcccg
      241 aacgtgcggc tccacacgct cgcgcagtac ttccgcacgg cggtccgacc gtcgcaccgc
      301 gccctgcccg acgccgaggc ctgctcggag gtgctccagg gcctgctcga gctcggcggg
      361 aggctcggga tcgtcacgct gggcgacctg cacggcgccg tccgcgcccg ggggcgtccg
      421 aacttcggga agatccggct ggccgaccat ctgccgaacg cgcccggcgt ctacctgttc
      481 cggggtcgcg acggccgcgt gttgtacgtc ggcaagtcgg tcgatctgcg ttcgcgggtg
      541 aagagctact tctacggcga cggtcgcaag aaggtcgagg acctgctcgc cgagaccgcg
      601 tccgtcgacg gcgttgcgtg tggctccgag ctcgagtccc tggtcctcga ggcgcgcctg
      661 atccgcgagc acgaaccgaa gtacaaccgt cggggcaaga cctggcggaa gtacgcctac
      721 ctgcggatcg acgacgccga ggcctacccg cggatcaagg tcgtgcgcga gaccaagggc
      781 ggcggcgcgt tcctcggccc gttcccctcg tcgcacctcg cgcgcctggc gaaggaggcg
      841 ctcgaggacg ccttcccgat ccgtcgctgc acgacggcga tgcgcgcatc cacgcggttc
      901 gcgccgtgcg cgctcgccga gatgcatcga tgcctgaccc catgcgacgg gcgcgtcggc
      961 cccgagcgct acgaggagct cgtccgctcc ctgatctcct ccctctccag accgggcggg
     1021 ctcctcggaa cgctcgaggc gcgcatgcgc gacctggccg agcaggaacg gttcgaggag
     1081 gccgcgctcg cgcgcgatcg cctccgcgcg ctcgcccagt cccttgcccg ggctcgggtc
     1141 gacgggtggc tgctcggcgc ggggcacctg gtcctgcgcg acgggcaggg gcgcctgttc
     1201 gacgttcggc gcggcgcgct gatccggacc gaacgcgacg agccgctcgg ctcaccgtgc
     1261 ccacgcgacc gggccgacga gctgtcggtg ctccggtcct ggatgtcgcg ccacgacgtc
     1321 cggatcgagg cggccgacgt cccgatcgcc gaacccgtcg acggaggcgc cgtcctgcac
     1381 cggatcctgc gcacgctccg cggcgcgacg ggcgaggacg gcggtgggct acgctcgctt
     1441 cgcgatgacc ggcgggcccg agctgctcct gcgcgagacg gatgacgcgt acgagatcct
     1501 gcttcgctgc atcggcgacc tgaccgacga ggagtacgcc tgggagcccg gcccggacgc
     1561 atggcgcgtg ttccgaggcg aggacggtcg gtggacgcac gactacgccg aacccgatcc
     1621 ggagccctcg ccgttcacga cgatcggctg gcgcgtcgcg cacgtggcga cgtgcaaggt
     1681 catgtaccac gagtgggcgt tcggcccgca gcggctgacc tggctcacga tcgagacgcc
     1741 gcacgacgtc gagggggcgc tcgcgatgct gcgggaggga caccagctgc tgcgcgccga
     1801 cctggcagcc ctcgacgagc ccggcctgaa cgccgaggtg ctgacgaact ggggcgagcc
     1861 ctggcccgcc tggcggatct tctggacgat gatccaccac gacctccagc acgggggcga
     1921 gatcggcgcc ctccgcgact acctgcgaac ctcgcccggg actcgatgac cgggcgggtc
     1981 ctcgcccgcg gctagacttg gtgcgtggag accgcggtca tcctcgaggg cgcccggacg
     2041 ccgatcggcc ggttcctggg ctcgttctcc gagctgcccg ccgtcgacct cggcgcgctc
     2101 gcggcccggg aggcgatgcg gcgctcgggc gtggaacccg accagatcga ccagacgatc
     2161 ctgggtcacg cgcgccaggc gggcaacggc ccgaacaccg gccgccaggt gagcgtccgc
     2221 gccggggttc cggtggaggt ctccgcgtac aacgtgaaca tcgcgtgcgg ctccgggatg
     2281 aaggccgtgc agctgggggc gcagcagatc gcgctcggcg actcgcaggt cgtgctggcg
     2341 gggggcatgg agaacatgac ccgggtcccc ttcctgctcg aaaggatgcg ctccggctac
     2401 cggatgggag acgagcgggt gtacgacgcg atgtaccgcg acgggctgct cgaccccctg
     2461 tgcgggctca tcatgggcga gaccgcggag aacctcgtcg accgctacgg gctgtcgcgg
     2521 gccgagcagg acgagttcgc gctcgagtcg cagcagaagg ccgccgcggc cgcgcgggcg
     2581 cgcgccgcgg agatgttcgc ggtcgaggcc ccgtcgggcg ggggcagcgt gaccgtgacc
     2641 gaggacgagc atccccgacc ggacacgacg ctcgagcggc tcgcggagct ggagcccgtg
     2701 ttccgcgagg gcggcagcgt caccgccggc aattcgagcg ggatcacgga cggagccgcc
     2761 gcgatggtgc tgatgtccgc gtcacaggcc gaggccgagg ggcgccaccc gctggcgcgc
     2821 atcgtcggga tggcgtgggc cggcgtgccg ccggagatca tgggtatcgg ccccgtgccg
     2881 gcgaccaaga gggtgctcga gaacaccggg ctctcgctcg ccgacatcga cctgatcgag
     2941 atcaacgagg cgttcgcggc gcaggtgctc gcgtgcgagc gcgagctgaa gttcgaccgc
     3001 gaccggctga acgtgcacgg cggcgggatc tcgctcggcc atccg
//
LOCUS       DATBRK010000454         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_399234, whole genome shotgun sequence.
ACCESSION   DATBRK010000454 DATBRK010000000
VERSION     DATBRK010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399234"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VIH70_13235"
     CDS             <1..891
                     /locus_tag="VIH70_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY8117371.1"
                     /translation="ADESDGSFLLGRPAVGVVTNIELDHVDFYVGGLEEIESAFTAFA
                     GRCAHVVVCADDPSAMRAVGAAGRPVTTYGTRPDAGIRVDVASVGPGGASAKLDSGGG
                     SHDLRLAVDGAHNLLNAAAAVAVAAAVGVPADAAVEALAGFAGVHRRFEHRGSARGAD
                     FYDDYGHVPTELEVTLDVARRTDHRRVLAVFQPHRYSRTQALWKELGASLVGADVIVV
                     TDVYGAAQEPIPGVTGKLVVEGIARTGCDRRVVYLPHRKDVVEFLAREVRRGDLVLTM
                     GCGDVWMLGDAALERIEEAS"
     gene            891..1829
                     /gene="murB"
                     /locus_tag="VIH70_13240"
     CDS             891..1829
                     /gene="murB"
                     /locus_tag="VIH70_13240"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY8117372.1"
                     /translation="MVTDGMVGRAEAILRAACGERVRTRFELAALTTFRIGGPAALFL
                     EPESESDLVAVSEAVRQTGIRVAVLGKGSNVLVADEGFPGLVLRLGRGYRWSARNGER
                     LTAGGAMPLPALAGVALAHGLSGLEFGVAIPASVGGAVRMNAGAHGGTLGEVVESVDV
                     FGLGDGAAQRVSADDTGFSYRRSDLGPGAVVVGATVRLRSGDPAEIRARMDEARDWRR
                     RTQPLAEPNCGSVFKNPPGDHAARLIEEAGAKGRRLGLASISTKHANFIIASDGATAA
                     DVVSLIREVQDIVAQRSGIRLEPEVHLLGDLDLASR"
     gene            1876..2544
                     /locus_tag="VIH70_13245"
     CDS             1876..2544
                     /locus_tag="VIH70_13245"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY8117373.1"
                     /translation="MGSIAVVIGGVASSRSSVFDVRRIEVSGADHLHRPQIVRIAGIS
                     NATNALWLDEGIAERRLEAEPWIADADVRVTLPLTVQITIHERSPVAVSRGAATSLMA
                     ADGTALGSGRVPRGLPVIDLGGAGSVEGIRPSPVGAATVLGAMPPALRAQVARVRVLL
                     DGTLELRLRAGPTVAFGTPEDAERKAVTIRWVLAWAGREGTPLRTLSVVSALAPAATF
                     IDPA"
     gene            2741..>3834
                     /gene="ftsZ"
                     /locus_tag="VIH70_13250"
     CDS             2741..>3834
                     /gene="ftsZ"
                     /locus_tag="VIH70_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY8117374.1"
                     /translation="MDSPQNYLAVIKVVGIGGGGVNAVNRMIEGGLRGVEFIAVNTDA
                     QTLLMSDAEVKLDIGRETTRGLGAGSDPEVGRRAVEEHRDEIEEILKGADMVFVTAGE
                     GGGTGTGGAPIVAEIARGLDALTIGVVTRPFGFEGRLRATQADLGITELKKAVDTLIV
                     VPNDRLLQVSDPSTPMIDAFRTADQVLFQGVDGITSLITTPGLINLDFADVRSVMTGA
                     GSALMGIGQGSGEDRAEEAARSAISSPLLESSIEGARGLLLSVAGPMGLTLHEVNRAA
                     DAITRVAHPDANIIFGAVIDDALGEEIRVTVIAAGFDKTLTATAPTTSRFAESRLSRL
                     LEEPEPLVVLEPVPAASTRIEDEDDVMPMPS"
BASE COUNT          531 a         1276 c         1435 g          592 t
ORIGIN      
        1 gcagacgaaa gcgacggttc cttcctcctg ggtcgtccgg ccgtcggcgt cgtcaccaac
       61 atcgagctgg atcacgtcga cttctacgtg ggcgggctcg aggagatcga gagcgcgttc
      121 accgcgttcg ccggacgatg cgcgcacgtc gtcgtctgcg ccgacgaccc gtccgcgatg
      181 cgcgcggtcg gggcggcggg tcggccggtc acgacgtacg gcacgcgacc ggacgcgggg
      241 atccgcgtgg acgtcgcctc ggtcgggccc gggggcgcat ccgcgaagct ggactcggga
      301 ggagggtccc acgatctccg gttggccgtg gacggcgcac acaacctgct gaacgcggcg
      361 gcggcggtgg cggtcgcggc ggcggtcggc gtccccgccg acgcggccgt cgaggcgctc
      421 gccgggttcg ccggggtcca tcgccgcttc gagcaccggg gatcggcgcg cggcgccgac
      481 ttctacgacg actacgggca cgtcccgacc gaactcgagg tgacgctgga cgtcgctcgc
      541 cggaccgacc accgtcgggt cctcgcggtc ttccagccgc accgctactc caggacgcag
      601 gccttgtgga aggagctcgg ggcgagcctg gtcggcgccg acgtcatcgt ggtgaccgac
      661 gtctacggcg ccgcgcagga gccgatcccc ggcgtgaccg gcaagctggt ggtcgagggc
      721 atcgcgcgca ccgggtgcga ccgtcgtgtc gtctacctgc cgcaccgcaa ggacgtcgtc
      781 gagttcctcg cgcgcgaggt tcgccggggc gacctggtcc tgacgatggg gtgcggcgac
      841 gtctggatgc tgggcgacgc cgcgctcgaa cggatcgagg aggcgtcgtg atggtgaccg
      901 acggcatggt gggccgggcg gaggcgattc tgcgggccgc gtgcggcgag cgggtccgca
      961 cgcggttcga gctcgcggcc ctcaccacgt tccggatcgg cgggcctgcc gccctcttcc
     1021 tggagcccga gtccgagtcg gatctggtcg cggtgtccga ggccgtacgc cagacgggca
     1081 tccgggtggc cgtgctcggg aaaggctcga acgtcctggt cgccgacgag gggttccccg
     1141 gcctcgtgct gcgtctcggc cgcggctacc gctggtcggc tcgaaacggc gagcggctca
     1201 ccgcaggggg cgcgatgccg ctgcctgcgc ttgcgggcgt tgccctcgcg cacgggctct
     1261 ccgggctgga gttcggggtg gcgatcccgg cctcggtggg cggcgccgtg cggatgaacg
     1321 ccggcgccca cggcgggacg ctcggagaag tggtcgagtc cgtcgacgtg ttcggattgg
     1381 gtgacggggc agcgcaacgc gtgagcgcgg acgacacggg cttctcctat cgccgctccg
     1441 atctcgggcc cggcgccgtc gtcgtcggag ccacggtccg gctgcgatcc ggcgatccag
     1501 ccgagatccg ggctcggatg gacgaggctc gcgattggcg ccgtcggacg cagccgctcg
     1561 ccgaaccgaa ctgcgggagc gtcttcaaga atccgcccgg tgaccacgcc gcccgtctga
     1621 tcgaggaggc gggggccaag ggtcgtcgtc tcggcctcgc ctcgatctcg acgaagcacg
     1681 cgaacttcat catcgcgtcc gacggtgcga ccgccgccga cgtcgtgtcc ctgatccgcg
     1741 aggtccagga catcgtcgcg cagcgatcgg gcatccgtct cgaaccagag gtgcatctgc
     1801 ttggggatct cgacctcgcg tcccggtgag tggttgcggt cgctccgcgg ccgtcgccga
     1861 acagcgttga tcgccgtggg atcgatcgcg gtcgtgatcg gtggggtggc ctcgtcgcgg
     1921 tcgtccgtgt tcgatgttcg ccggatcgag gtgtcgggcg ccgatcacct gcatcggccc
     1981 cagatcgtcc ggattgccgg catctcgaac gcgaccaacg cgctctggct cgacgaaggg
     2041 atagccgagc gccgtctgga ggccgagcca tggatcgcgg acgcggacgt ccgtgtgacc
     2101 ctcccgctga cggtgcagat caccatccac gaacgctcac cggtcgccgt ctcgcgggga
     2161 gcggcgacgt cgctgatggc cgccgacggg accgcgctcg gatcggggag ggtcccccgg
     2221 ggcctcccgg tgatcgacct cggcggtgcg gggagcgtcg agggcatccg accgagcccg
     2281 gtcggcgccg ccacggtgct cggcgcgatg ccgcccgccc tgcgggcgca ggtcgcccgg
     2341 gtacgggtcc tgttggacgg gacgctcgag cttcgtctgc gggctggccc gaccgttgcc
     2401 ttcggcacgc cggaggacgc ggagcgcaag gccgtgacga tccggtgggt gctcgcgtgg
     2461 gccgggagag aggggacgcc cctgcgaacc ctctcggtcg tctccgcact cgcgccggcc
     2521 gcgacgttca tcgatccggc ctgatcgagc ggttgttacg cgcacgacta acgcgaccag
     2581 tggggatatt gacggaccag ggctttgata gtagcgtgcc gtagtcaacg tcaagtagat
     2641 ataacaatca acgttcagtt gaggttcagt ccttcgggag acacaacggc gtttccccag
     2701 gtagaccggg ggcttcaccc ggggagcgga gatctcggtg atggattctc ctcaaaacta
     2761 cctcgccgtc atcaaggtgg tcgggatcgg cgggggcggt gtgaatgcgg tcaaccgcat
     2821 gatcgagggc ggcctgcgcg gggtcgagtt catcgcggtg aacacggacg cgcagacgct
     2881 gctgatgtcg gacgcggagg tcaaactcga catcggccgt gagacgacac gcggcctcgg
     2941 cgcgggttcg gaccccgagg tcggtcgccg ggcggtcgag gaacaccgcg acgagatcga
     3001 agagatcctc aagggcgccg acatggtgtt cgtcacggcg ggcgagggcg ggggcaccgg
     3061 gaccggcggc gcgcccatcg tggccgagat cgcccgcggg ctcgacgcgc tcacgatcgg
     3121 cgtcgtgacg cggccgttcg ggttcgaggg tcgtctgcgc gcgacgcagg cggacctcgg
     3181 gatcaccgag ctgaagaagg ccgtcgacac gttgatcgtg gttcccaacg atcgcctgct
     3241 ccaggtctcc gaccccagca ccccgatgat cgacgcgttc cgcaccgcgg atcaggtcct
     3301 gttccagggt gtggacggca tcacgtcgct catcacgacg ccgggcctga tcaacctcga
     3361 cttcgcggac gtcaggtcgg tcatgacggg cgcggggtcc gcccttatgg ggatcggtca
     3421 gggctcgggc gaggaccgcg ccgaggaggc cgctcggtcg gcgatctcgt cgccgctgct
     3481 ggaatcctcg atcgagggcg cgcgcgggtt gctgctgtcg gtcgccgggc ccatgggtct
     3541 caccctgcac gaggtgaaca gggccgcgga cgcgatcacc cgggtcgcgc accccgacgc
     3601 gaacatcatc ttcggcgccg tgatcgatga cgccctgggc gaggagatcc gcgtgacggt
     3661 gatcgccgcc gggttcgaca agacgctgac cgcgaccgcg ccgacgacgt ccaggttcgc
     3721 cgagagccga ctgtcgcgcc tgctcgagga gcccgagcct ctggtggtgc tcgagccggt
     3781 cccggcggca tccacgcgga tcgaggacga ggacgacgtc atgccgatgc cgtc
//
LOCUS       DATBRK010000455         4597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1567634, whole genome shotgun sequence.
ACCESSION   DATBRK010000455 DATBRK010000000
VERSION     DATBRK010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567634"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..999)
                     /locus_tag="VIH70_13255"
     CDS             complement(55..999)
                     /locus_tag="VIH70_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8117375.1"
                     /translation="MKIFFVTDLHGSEICWKKFLNAGPFYKADVVILGGDITGKAMVP
                     IVQRPSGSWEASLQDHLETFETDEEIVEFEKRVMNRGYYPICVSQEEYVAMQEDADLV
                     DKRFKEVMLDGTERWIAMAEDKLAGTGIRVVACPANDDMFEIDELLTHAHVVETGDED
                     HPIELDGFTMISMGWTNPTPWHTFREAEEPELAVRIENALSHAGDPETTIFNFHAPPY
                     GSKLDNAPALNEDLTYKSGGQALRPVGSTSVKEAIERFQPMLGLHGHIHESKGAARIG
                     STLSLNPGSSYEEGILQAAVVTIDAKKRKVKNYQLVNG"
     gene            complement(1052..1810)
                     /locus_tag="VIH70_13260"
     CDS             complement(1052..1810)
                     /locus_tag="VIH70_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117376.1"
                     /translation="MDAPVRDAFAESIEVVRAANARGIPLKLIGGQAVRLLCPLFPHR
                     AREDQDMDFACVSPKKRDVIAFFGERGFQGDARFNTLHGDRQMYFTTADGNTSVDVVM
                     DKLNMCHVLDFSERIDRMPDTLDVTDLLLSKLQIVELNEKDVHDLLHLLSGFEAMPGD
                     DPGTIGLGRIGRQVASDWGWWRTTTMNLDKLAQLADGDHRDLLPEARRFEPAEQARRV
                     RAFCDEVPKTMKWKLRARVGDRVRWYELPEEVGH"
     gene            1917..3569
                     /gene="betA"
                     /locus_tag="VIH70_13265"
     CDS             1917..3569
                     /gene="betA"
                     /locus_tag="VIH70_13265"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY8117377.1"
                     /translation="MTAGRYDFVIVGGGSAGCALANRLSTDPSNRVLVLEAGRPDYPW
                     DVYIHMPAALTFPIGNRFYDWRYESEPEPHMRGRRIYHARGKVLGGSSSINGMIFQRG
                     NPLDFERWAADPGMQAWDYAHCLPYFRRMEHCLAAGPGDEFRGAAGPLVLERGPATNP
                     LFGAFFEAARQAGFELTDDVNGYRQEGFAAFDRNIHRGRRLSAAGAYLHPVRSRPNLE
                     VRTRTFVSRILFEGSRAVGVEAGRGRAAPARIDAGEVIVCGGAINTPQTLQLSGVGPA
                     EELRALGIDVVHDLPGVGENLQDHLEVYVQHACTQPVSMAPYLKWRYRPWIGFRWLFF
                     RSGPGATNHFEGGGFARSNDEVRYPNLMFHFLPIAVRYDGSAPASDHGYQVHIGPMYS
                     DARGSVRITSPDPRVHPALRFNYLSTDQDRREWVEAIHTARRILGQPAMAPFDGGEIS
                     PGPGVQTDEQILAWVAGDAETALHPSCTCKMGTTEMSVVDPSTMRVHGLDGLRVVDAS
                     VFPYVTNGNIYAPVMMVAEKAADLILGNTPLAPETVEFFRRT"
     gene            complement(3591..4457)
                     /locus_tag="VIH70_13270"
     CDS             complement(3591..4457)
                     /locus_tag="VIH70_13270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8117378.1"
                     /translation="MIFGLAAAFGWGLGDLWAGMAGRRIGSGRTVVVAQVSVAIVATL
                     LVIVVRPDLSGLPRVVPWLVPAAFVGAAAFATLYRGLELGPIAVVSPVLATYAVVPVF
                     LSVVLLDESLGALEWAGAAITIAGAVLTSTDLRALRGGSFARFAGLPWAIASTLLFGV
                     ATYVLGWSAKEAGFLASMWFARGTVSIVVVVAAFVVWVRSNRRSAVPVRRSPSALRLA
                     ALGGVVELLGTLVYARGAEVGLVSVVTAASATYPFIPVLGGVVLLHERLVPNQYVGVA
                     LVVAGLMLLAFG"
BASE COUNT          721 a         1679 c         1514 g          683 t
ORIGIN      
        1 gagcttcgcc taccgctgcc atctggaccc cctcccgagg tacggaacgg cgatctagcc
       61 gttcaccagt tggtagttct tcaccttccg cttcttcgcg tcgatcgtca cgacggccgc
      121 ctggaggatc ccctcctcgt aggagctgcc cgggttgagc gacagcgtcg acccgatcct
      181 cgcggcgccc ttgctctcgt ggatgtgccc gtgcaggccg agcatcggct ggaaccgctc
      241 gatcgcctcc ttcaccgagg tcgagccgac cggccgcagc gcctgcccac ccgacttgta
      301 cgtgaggtcc tcgttcagcg ccggcgcgtt gtcgagcttg gagccgtacg gcggcgcgtg
      361 gaagttgaag atcgtggtct ctgggtcacc cgcgtgtgaa agcgcgttct cgatccgaac
      421 cgcgagctcg ggctcctcgg cctcccggaa cgtgtgccag ggcgtcgggt tcgtccaccc
      481 catcgagatc atcgtgaacc cgtcgagctc gatcgggtgg tcctcgtccc cggtctccac
      541 gacgtgggcg tgggtcaaca gctcatcgat ctcgaacatg tcgtcgttgg ccgggcacgc
      601 cacgacccgg atccccgtgc cggcgagctt gtcctcggcc atcgcgatcc agcgttcggt
      661 gccgtcgagc atgacctcct tgaaccgctt gtcgacgagg tcggcgtcct cctgcatcgc
      721 gacgtactcc tcctgcgaga cgcagatggg gtagtagccg cggttcatca cgcgcttctc
      781 gaactcgacg atctcctcgt cggtctcgaa cgtctcgagg tggtcctgga gggaagcctc
      841 ccaggagccg ctggggcgct gcacgatcgg caccatcgcc ttgcccgtga tgtccccgcc
      901 caggatcacc acgtcggcct tgtagaacgg ccccgcgttc aggaacttct tccagcagat
      961 ctccgacccg tgcaggtcgg tcacgaagaa gatcttcact cacgccatct tccgcccggc
     1021 cgcccaaggg cgcaagcggg ccgggacggg cctagtggcc gacctcctcg ggcagctcat
     1081 accaccgcac gcgatcgccc acccgggccc ggagcttcca cttcatggtc ttgggcacct
     1141 cgtcgcagaa ggcccgcacc cgtcgggcct gctcggcggg ttcgaagcgg cgtgcctcgg
     1201 gcagcagatc tcggtggtcc ccgtcggcga gctgcgccaa cttatccagg ttcatcgtgg
     1261 tggtcctcca ccacccccag tccgacgcca cctggcggcc gatgcgcccg aggccgatcg
     1321 tgccggggtc gtcaccgggc atcgcctcga accccgagag taggtggagc aggtcgtgga
     1381 cgtccttctc gttcagttcg acgatctgca gtttggacag caggaggtcg gtgacgtcca
     1441 gcgtgtcggg catccgatcg atccgctctg agaaatccag gacgtggcac atgttcagct
     1501 tgtccatgac gacgtcgacc gaggtgttcc cgtccgcggt cgtgaagtac atctgccggt
     1561 cgccatggag cgtgttgaag cgcgcgtccc cctggaaccc ccgctcgccg aagaaggcga
     1621 tcacgtcccg cttcttcggc gacacgcacg cgaagtccat gtcctggtcc tcgcgcgccc
     1681 ggtgcgggaa gagcgggcac aggagccgga cggcctgacc accgatgagc ttgagcggga
     1741 tcccccgagc gttggcggcg cgaaccacct cgatcgactc cgcgaaggcg tctcggaccg
     1801 gcgcgtccat ccgggcccga gcttaccccc gccgtccggc gagccgccca cggccgagcg
     1861 cgcgcgactc gacctgaggc acacttcgag acgtcggcag gcgccagaaa ggagcgatga
     1921 cggccgggcg gtacgacttc gtgatcgtgg gcggcgggtc cgccgggtgc gcgctcgcca
     1981 accggctctc gaccgacccg tccaaccgcg tcctggtcct ggaggccgga cggcccgact
     2041 acccgtggga cgtctacatc cacatgccgg cggcgctcac gttcccgatc ggcaaccgct
     2101 tctacgactg gagatacgag tccgaacccg aaccccacat gcgcggccgc cgcatctacc
     2161 acgcccgggg caaggtgctc ggcggctcga gcagcatcaa cggcatgatc ttccagcgcg
     2221 ggaacccgct cgatttcgag cgatgggccg ccgaccccgg gatgcaggcg tgggactacg
     2281 cgcactgcct cccctacttc cgccggatgg agcactgcct cgcggccggg cctggcgacg
     2341 agttccgagg cgccgcgggc cccctcgtgc tcgaacgcgg cccggccacc aaccccttgt
     2401 tcggcgcgtt cttcgaggct gcgcgtcagg cgggcttcga gctgaccgac gacgtgaacg
     2461 gctaccgaca ggaggggttc gcggcgttcg accggaacat ccaccgcggc cggcggctgt
     2521 cggcggcggg cgcctacctg cacccggtga ggtcgcggcc gaacctcgag gtccgaacgc
     2581 ggacgttcgt gtcccggatc ctgttcgagg gctcgcgcgc cgtcggggtc gaggcggggc
     2641 ggggccgcgc cgcgcccgcg cggatcgacg ccggcgaggt catcgtctgc ggcggcgcga
     2701 tcaacacacc gcagaccctg cagctctcgg gcgtcggccc ggccgaggag ctccgagcgc
     2761 tcgggatcga cgtggtgcac gacctgcccg gcgtcggcga gaacctgcag gatcacctcg
     2821 aggtctacgt ccaacacgcg tgcacgcagc cggtctcgat ggcgccgtac ctgaagtggc
     2881 ggtatcggcc ctggatcggg ttccgctggc tgttcttccg ctcggggccg ggcgcgacga
     2941 accacttcga gggcggcggg ttcgcccgca gcaacgacga ggtccggtac ccgaacctga
     3001 tgttccactt cctcccgatc gcggtccggt acgacggctc ggcgccggcg agcgatcacg
     3061 gctaccaggt ccacatcggg ccgatgtact ccgacgcgcg gggctcggtg aggatcacga
     3121 gtcccgatcc ccgcgtccac ccggccctgc gcttcaacta cctgtcgacc gaccaggacc
     3181 ggcgcgaatg ggtggaggcg atccacacgg cccggcggat cctgggtcag ccggcgatgg
     3241 cgccgttcga cggcggcgag atctcgcccg gccccggcgt gcagacggac gagcagatcc
     3301 tcgcgtgggt cgccggcgac gcggagaccg ccctgcatcc ctcgtgcacc tgcaagatgg
     3361 ggaccacgga gatgtcggtg gtcgatccct cgacgatgcg cgtccatggg ctggacggac
     3421 tccgcgtcgt cgacgcgtcg gtcttcccct acgtcacgaa cggcaacatc tacgctccgg
     3481 tcatgatggt ggccgagaag gcggccgacc tgatcctggg aaacactccc ctcgcgcccg
     3541 agacggtcga gttcttccgc cggacatgag cccggtcttg gggatgtccg tcagccgaac
     3601 gcgagcagca tcagcccggc gacgacgagc gccacgccca cgtactgatt gggcaccagg
     3661 cgctcgtgga ggagcacgac gccgcccaac acggggatga acgggtaggt ggccgacgcg
     3721 gccgtaacca ccgataccag gccgacctcg gcgccccgcg cgtacaccag cgttccgagc
     3781 agctcgacca cccctccgag cgcggcgagc cgaagcgccg atggcgagcg ccggaccgga
     3841 accgcgcttc gccggttcga tcgaacccag acgacgaacg cggcgacgac cacgacgatc
     3901 gacacggtcc cccgcgcgaa ccacatcgac gcgaggaatc cggcttcctt ggccgaccac
     3961 cccagcacat aggtcgcgac cccgaacagt agggtcgacg cgatcgccca gggcaacccg
     4021 gcgaaccgcg cgaacgatcc gccgcgcagg gcccgcaggt cggtcgatgt cagcacggcc
     4081 cccgcgatcg tgatcgccgc ccccgcccac tccagcgctc cgagcgactc gtccagcagg
     4141 accaccgaga ggaagaccgg aacgacggcg taggtggcaa ggaccgggga gaccaccgcg
     4201 atggggccga gctcgagacc ccgatacagc gtcgcgaacg ccgcggcgcc gacgaacgcg
     4261 gccgggacga gccacggcac gaccctgggg agcccggaca gatccggccg aaccacgatc
     4321 acgagcaggg tcgccacgat cgcgacggag acctgcgcga ccacgaccgt ccggccgctc
     4381 ccgatcctgc ggcccgccat ccccgcccac aggtctccga gcccccaccc gaacgcggcg
     4441 gcgagcccga agatcacccc ccgccccgat accgcgcgtc ccgccaggcg atcgcggcct
     4501 tcagaccctc ctcttgggtg atccgccgga actcgtcgcg ggccggcagg ttggcggtct
     4561 cgatcatcac gtcgagctcg gtgttggcgt cgagcgc
//
LOCUS       DATBRK010000456         4273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_129161, whole genome shotgun sequence.
ACCESSION   DATBRK010000456 DATBRK010000000
VERSION     DATBRK010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129161"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /locus_tag="VIH70_13275"
     CDS             <1..1711
                     /locus_tag="VIH70_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY8117379.1"
                     /translation="IGKRTAFAEWVMAYDMLDEGLIDEDEALLRVDANRLEELFKRRV
                     KADGARPIAKGLNASPGAATGAVVFTADDAQERANAGDKVVLVRRETTPDDYHGMIAS
                     QGILTSAGGTNSHAAVVARGEGIPAVCGADAIKLDSVAKTFTANGTTVQEGEVVTIDG
                     FTGNVYVGELPLEESVLEKARSGDEEARGEKIWKAFERLMSVADARRRLRVRANADTP
                     DQSTNARDRGAEGIGLCRTEHMFLGEERVGAVRQMIFAETEAEEQKAYDALLPLQRED
                     FVAIFRAMDGLPVTVRLLDPPLHEFLPNQVELAVGVARAEERGEDEVRLRDERVPLDE
                     ARVILGKVDQLHEANPMLGLRGVRLGIVKPGLYAMQVRAICEAAARVKQEGRDPKVEI
                     MIPLVATKPELEQMRAELEPVAAEVLRAEGVQLDHLEWGTMIELPRATVTSGEIAEAA
                     EFFSFGTNDLTQTAFGFSRDDIGKFVGMYEERKLVPANPFVTIDQPGVGRLMRIAVEE
                     GRAANASLHVGICGEHGGDPASVEFCHQLGLDYVSCSPFRVETARLAAGQAAVGSAES
                     ASR"
     gene            1940..2473
                     /locus_tag="VIH70_13280"
     CDS             1940..2473
                     /locus_tag="VIH70_13280"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY8117380.1"
                     /translation="MLPLIVAFHWARLGWRDEHVNYPSRPSGYTQIVNRFGQPCNAEA
                     RAIPMRWTAADTGVTYTIRFHHKLGGRPTAMVSDKGGRSTNLDNDVYGHIQNAHVQPY
                     IKHGIYGYTCRAKRNSNEWSTHAFGIAIDVSSAEEYMGKCTSTVNKNHASIWQNHGWY
                     WGLAFCDPMHFQYATNY"
     gene            2492..2995
                     /locus_tag="VIH70_13285"
     CDS             2492..2995
                     /locus_tag="VIH70_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117381.1"
                     /translation="MKRTQAIVVGLVVGVTASAGVVAGAAGKAPVRPSHGPPVGPMIR
                     ADTGAGELLLAVVGGVFSSRAEAEAANETLPFGDLQGYYVVPVGQFQGFRQQVGQAGD
                     FALVSAFRTEEGAQDFVELAATLGNAATILPERVRSLGGLYAGLGQEASPDGTGPLLG
                     PVAESLP"
     gene            2992..3444
                     /locus_tag="VIH70_13290"
     CDS             2992..3444
                     /locus_tag="VIH70_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117382.1"
                     /translation="MTGARNAAAFVSGVLALVACGGGAGTQPVPTAQRTVCEVAFVTP
                     PGFERTDTFEEPYPDRLGLRIGFRDEAGRELHAFAGIPGEFGEGLPEAGAVELVGGRT
                     GLLAGGPHRVWVLTWDEGGLCDPRAVLGRGFDRGEFLDLLALAGVARM"
     gene            3484..4092
                     /locus_tag="VIH70_13295"
     CDS             3484..4092
                     /locus_tag="VIH70_13295"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY8117383.1"
                     /translation="MEAVRRHPVLITIAAVAAAIVIAFAGTGVAVWRAAHTDDASTIE
                     HVDAILVLGAAQYDGEPSPVFEGRLEHARLLYEEGRAETILVLGGGAPGDRTTEAEAG
                     REWLVSRGVPSDAVVASPFGSTTFESLEAAAAWMNERGMKAAFLVSDPWHNLRIKRMA
                     SDLGLEAYASATWHSAARTEGTRFGGYLRETLAYAFYRLFGR"
BASE COUNT          658 a         1386 c         1600 g          629 t
ORIGIN      
        1 catcggcaag cggacggcgt tcgccgaatg ggtgatggcc tacgacatgc tcgacgaggg
       61 cctgatcgac gaggacgagg cgctgctccg cgtcgacgcg aaccggctcg aggagctgtt
      121 caagcggcgc gtgaaggccg atggcgcccg gccgatcgcg aagggcctca acgcgagccc
      181 cggcgccgcc acaggcgcgg tggtcttcac ggccgacgac gcgcaggagc gagcgaacgc
      241 cggcgacaag gtggtcctcg ttcggcgcga gaccaccccg gacgactacc acggcatgat
      301 cgcctcgcag gggatcctga cgagcgccgg gggaaccaac tcgcacgcgg cggtcgtcgc
      361 gcgcggcgag gggatccccg ccgtctgcgg ggccgacgcg atcaagctcg actccgtggc
      421 gaagacgttc accgcgaacg gcacgaccgt gcaggaaggt gaggtcgtca cgatcgacgg
      481 cttcacgggc aacgtgtacg tcggggagct accgctcgag gagtccgtgc tcgagaaggc
      541 gcgatccggc gacgaggagg ctcgcggaga gaagatctgg aaggccttcg agcggctgat
      601 gagcgtcgcc gacgcgcgcc ggcgcctgcg cgtccgcgcg aacgcagaca cgcccgacca
      661 atcgaccaac gcgcgcgacc gcggagccga aggcatcggg ctgtgccgga ccgagcacat
      721 gttcctcggc gaggagcgcg tgggcgcggt gcgacagatg atcttcgcgg agaccgaggc
      781 ggaggagcag aaggcgtacg acgccttgct gccgctgcag cgcgaggact tcgtcgcgat
      841 cttccgggcc atggacggcc tgccggtcac cgtccggctg ctcgaccccc cgctgcacga
      901 gttcctcccg aaccaggtcg agctcgcggt cggcgtggcc cgggccgagg agcgcggcga
      961 ggatgaggtc cggctgcgcg acgagcgggt gccgctcgac gaggcccggg tgatcctcgg
     1021 caaggtcgac cagctccacg aggccaaccc gatgctcggc ctgcgcggtg tgcgcctggg
     1081 gatcgtgaaa cccgggctgt acgcgatgca ggtccgcgcg atctgcgagg cggcggcacg
     1141 ggtcaagcag gagggccgcg acccgaaggt cgagatcatg atcccgctcg tcgcgaccaa
     1201 accggagctc gagcagatgc gcgccgagct cgaaccggtt gcggcggagg tgcttcgggc
     1261 cgagggcgtc cagctcgacc acctcgagtg gggcacgatg atcgagctcc cgcgcgcaac
     1321 ggtcacctcg ggggagatcg cggaggcggc cgagttcttc tcgttcggca ccaacgacct
     1381 cacgcagacg gcgttcggtt tcagccgcga cgacatcggc aagttcgtcg ggatgtacga
     1441 ggaacgcaag ctcgtgccgg ccaacccgtt cgtcacgatc gatcagccgg gggtcggtcg
     1501 cctgatgcgg atcgcggtcg aggagggccg ggccgcgaac gcgagcctgc acgtcgggat
     1561 ctgtggcgag cacggcggcg acccggcatc ggtcgagttc tgccaccagc tcggcctgga
     1621 ctacgtgagc tgctcgccgt tccgtgtcga gacggcccgc ctcgccgccg gccaagccgc
     1681 ggtgggttcg gcggagtccg cgtcgaggtg acggacctcg atgacgacga acgtctgacg
     1741 cggccgcgtc gctcggcgtg gaatggtcct cgctcgccgc gcgaatcccc cggcttggcg
     1801 atgcgcggtc gaacgcgcgt tcgctaagct ccgagacctc tcccgcctcc cccgggttcc
     1861 gaggaggtgg ctcgtgcgga caggttcgca tagaggggga gggctgcggt tcctcagcac
     1921 cgcgagaccc atcgcggtcg tgctgccgtt gatcgtggcg ttccattggg cgcgcctcgg
     1981 ttggcgggac gagcacgtca actacccgtc gcggccgagc ggctacacgc agatcgtcaa
     2041 tcggttcggg cagccgtgca acgcggaggc cagggcgatc ccgatgaggt ggaccgcggc
     2101 ggacaccggg gtgacgtaca cgatcaggtt ccatcacaag ctcggcggcc gtccgacggc
     2161 gatggtctcg gacaagggcg ggagatcgac caacctcgac aacgacgtct acgggcacat
     2221 ccagaacgcc cacgtgcagc cgtacatcaa gcacgggatc tacgggtaca cgtgccgggc
     2281 caaacggaat tccaacgagt ggagcaccca cgcgttcggg atcgccatcg acgtgtcgtc
     2341 ggccgaggag tacatgggca agtgcacgtc gacggtcaac aagaaccacg cgtcgatctg
     2401 gcagaaccac ggctggtact ggggactggc gttctgcgac cccatgcact tccagtacgc
     2461 caccaactac tgagttcctc gaggaggcat catgaagcgg acacaagcga tcgtcgtcgg
     2521 actcgtggtc ggcgtgacgg cctcggcggg ggtggtcgcg ggcgccgccg ggaaggcgcc
     2581 cgttcggccg tcgcacggtc ctcccgtcgg tccgatgatt cgcgcggata ccggagcggg
     2641 cgagctgctg ctcgccgtcg tgggcggggt cttctcgagc cgggcggagg cggaggcggc
     2701 caacgagacg cttccattcg gggacctcca gggctactac gtggtcccgg tcggccagtt
     2761 ccaggggttt cggcagcagg tgggccaggc gggtgatttc gcgctcgtga gcgcgttccg
     2821 taccgaggag ggcgcccaag acttcgtcga gctcgccgcg acgttgggga acgcggcaac
     2881 gatcctgccc gagagggtgc ggagcctcgg cgggctctac gcgggtctgg ggcaggaggc
     2941 cagccccgac gggacgggac cgctgctcgg accggtggcg gagtcgcttc cgtgacgggt
     3001 gcacggaacg ccgcggcctt cgtgtcgggt gtgctggcgc tcgtcgcgtg tgggggcggg
     3061 gccgggacgc aacccgtgcc gaccgcgcaa cggaccgtgt gtgaggtggc cttcgtcacc
     3121 ccgccggggt tcgagcggac ggacacgttc gaggagcctt atccggatcg actcggtctg
     3181 cggatcggct tccgagacga ggcgggacgg gagcttcacg cgttcgccgg catcccgggc
     3241 gagttcggcg aaggcctccc cgaggccggg gccgtcgagc tcgtcggcgg ccggaccggg
     3301 cttctcgccg gcggtcccca ccgggtgtgg gtcctcacct gggacgaggg cggtctgtgc
     3361 gacccgaggg ccgtcctggg tcggggattc gaccgaggcg agttcctcga tctgctcgca
     3421 ctcgccggcg ttgcacgcat gtgagagatc tcgttcggga gaggcgttgt tagcatcgcc
     3481 tccatggagg cggtccgtcg acaccccgtg ctgatcacga tcgccgcggt cgccgcggcg
     3541 atcgtgatcg cgttcgcggg cacgggggtc gccgtctggc gggcggcgca cactgacgac
     3601 gccagcacga tcgagcacgt ggacgcgatc ctcgtcctcg gcgccgcgca gtacgacggc
     3661 gaaccgtcgc cggtgttcga gggccggctc gaacacgcga ggctgctgta cgaggagggc
     3721 cgggccgaaa cgatcctggt gctcggcggc ggcgcacccg gcgaccggac gacggaggca
     3781 gaggcgggac gcgagtggct ggtctcgcgg ggtgttccct cggatgcagt ggtggcgtcg
     3841 ccgttcggtt cgaccacgtt cgagagcctc gaggcggccg cggcgtggat gaacgagcgg
     3901 gggatgaagg cggcctttct cgtctcggat ccctggcaca acctgcggat caagcggatg
     3961 gcctccgatc tcggcctcga ggcgtatgcg tcggcgacgt ggcactcggc ggctcggacg
     4021 gagggaacgc ggttcggggg atacctccgc gagacgctcg cgtacgcctt ctaccggctg
     4081 ttcgggcgct gagcggctcg gacccctagc tcaccttcag cgtcgccctc atgccgaggg
     4141 attcgtggaa ccggcagatc agctggtacg tccccggcgc cagatcgatc ttcacggtct
     4201 cggtcgtgag cggggcgagc tcgaggtcga cgtcctcccc gacgaccgtg aacgtgtgcg
     4261 gcgtcctcgc gtt
//
LOCUS       DATBRK010000457         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_834861, whole genome shotgun sequence.
ACCESSION   DATBRK010000457 DATBRK010000000
VERSION     DATBRK010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834861"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..226
                     /locus_tag="VIH70_13300"
     CDS             2..226
                     /locus_tag="VIH70_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117384.1"
                     /translation="MPQSAAPAWASVPGIEVRRLTSDKPYRCPGCDLEIRPGTWHLVV
                     VPAVPEERRHWHERCWQVELNRTRGRRPGG"
     gene            257..610
                     /locus_tag="VIH70_13305"
     CDS             257..610
                     /locus_tag="VIH70_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117385.1"
                     /translation="MPPPPVRASMRHEPPVWVGRVRGREIRRRTRDSCLNGLRRLAGD
                     DALLWVEVTPDVVGVSEAATILGWDRRRVATYVSRGAFPEPIAALASGRVWRREDVEA
                     FGRDRLRRRGRRIAR"
     gene            607..1254
                     /locus_tag="VIH70_13310"
     CDS             607..1254
                     /locus_tag="VIH70_13310"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8117386.1"
                     /translation="MTQRILYLVRHGESDFDSTRTTPSARGEQWDPPLGERGREQARL
                     LAGRLRAMPEPAVVASSPLLRARETASAYAEPAGIEVRVDDDLAEAHLGEWEGKSFEE
                     ILSTDEQMLHRVRNQEPIWRHAPFVEELGPFRARVRAVVERLLAAHPDGNVLVFCHGG
                     VINAYIAPLLGVEHEMFFVPENTSLNSIVVEGDRRRVRFLNDALHLTDPHLFADA"
     gene            1319..1702
                     /locus_tag="VIH70_13315"
     CDS             1319..1702
                     /locus_tag="VIH70_13315"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY8117387.1"
                     /translation="MPVQFLSPEWTEELEKRLNASDEFRAAAAAANVTIQNVIATPDG
                     AKRYWMRFEGGSVTLEAGDAEKADATIEQDYDTAVALAKSELNPVSAFMTGKIRINGS
                     MMLLMQLQGALSELAKEMQEIDVDY"
     gene            1874..2989
                     /locus_tag="VIH70_13320"
     CDS             1874..2989
                     /locus_tag="VIH70_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8117388.1"
                     /translation="MRSWRAKMLVLIGVLSIVAAACGNDEGDETGGGGGTATGGGAAE
                     CNADLKVGVAFDIGGLGDKSFNDSANAGLQQAIADGLVCEDNAILNEANSTGSDRDQN
                     VQALADAGMNLVVGVGFAFSPGINTIAPDYPDVDFMIVDGFATCGTACGLTNDAEAIP
                     NVADYTFKEQEGSFLVGAAAVLKCQCKTIGFLGGQQGTGLIEKFEAGYEAGAHQIDPD
                     VEILVEYIGDSTEAFNDPVKGEELSTKMYDQGAEIIYHASGASGAGLFNAAVEAQKLA
                     IGVDSDQYLTASPEQQPLILTSMIKRVDVAVYNAIEAEGNDTFGGSQVFGMAEDGVDY
                     ATSNPEELTADITEQLDEFKQQIIDGTIVVPDTPEGA"
     gene            3008..>3435
                     /locus_tag="VIH70_13325"
     CDS             3008..>3435
                     /locus_tag="VIH70_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8117389.1"
                     /translation="MTIGAGAGSNGSPPLVVLEHITKRFPGVVANDEVDLELRSGEVH
                     ALIGENGAGKSTLMRVLYGMYPSDGGRILVRGDEVKVASPRDAIGLGIGMVHQHFVLV
                     DPFTVTENIILGEEGGERLDMDAAQQRVSDLAETYAFRVDP"
BASE COUNT          569 a         1136 c         1228 g          502 t
ORIGIN      
        1 catgccgcag tcggcggcgc cggcctgggc gagcgttccg ggcatcgagg tgcgtcggct
       61 gacgagcgac aagccctatc gctgcccagg atgcgacctc gagatccggc cgggcacgtg
      121 gcacctcgtc gtggtccccg cagtccccga ggagcgtcgt cattggcacg agcgctgctg
      181 gcaagtcgag ctcaaccgga cgcgcggtcg ccgtccgggt ggctgaacgg cgttcggccc
      241 cggctatcat cggtgcgtgc cgccaccacc cgtgcgggcg tcgatgcgtc acgagccacc
      301 ggtctgggtg ggcagggtcc gcggtcgcga gatcaggcgg cgcacccggg acagctgcct
      361 gaacggcctg cggcgcctcg cgggcgacga cgccttgctg tgggtggagg tcacccccga
      421 cgtcgtgggc gtgagcgagg ccgcgacgat ccttggctgg gatcgccggc gcgtggcgac
      481 gtacgtgagc cggggagcgt tccccgagcc gatcgcggcc ctcgcgtccg ggcgcgtctg
      541 gcggcgcgag gacgtcgagg ccttcggtcg cgaccgtctc cggcgcaggg gccgcaggat
      601 cgcccggtga cccaacggat cctgtatctg gtccgccacg gcgaatccga cttcgactcc
      661 actcgaacga cgccgagcgc tcgtggcgag caatgggacc caccgctcgg cgagcgggga
      721 cgggagcagg cgcgcctgct cgccggccgg ctccgcgcca tgcccgagcc cgccgtcgtc
      781 gcctcctcgc ccctgctccg ggctcgcgag accgcgtcgg cgtacgcgga gcccgccggg
      841 atcgaggtgc gtgtggacga cgacctcgcc gaggctcacc tgggcgagtg ggagggcaag
      901 tcgttcgagg agatcctctc gacggacgag cagatgctgc accgggtccg taaccaggaa
      961 ccgatctggc gtcacgcccc gttcgtcgag gagctcgggc cgttccgggc gcgcgtccgc
     1021 gccgtcgtcg aacgactgct ggctgcccac ccggacggca acgtcctcgt gttctgccac
     1081 ggcggggtga tcaacgccta catcgcgccg ttgctcggcg tggagcacga gatgttcttc
     1141 gtgcccgaga acacgtcgct caactccatc gtggtcgagg gtgaccggcg ccgggtcagg
     1201 ttcctcaacg acgcgctcca cctgaccgac ccccacctgt tcgcggacgc ctgaggcgct
     1261 cgagccctag actccgcgtc gatcacggct cgtacccacc cccggacagg aggccgacat
     1321 gcccgtccag ttcctttcgc ccgagtggac cgaggaactc gagaagcgcc tgaacgcgag
     1381 cgacgagttc cgggccgcgg ccgccgccgc gaatgtgacg atccagaacg tgatcgccac
     1441 cccggacggc gcgaagcgct actggatgcg gttcgagggg gggtcggtca cgctcgaggc
     1501 cggcgacgcg gagaaggccg acgccacgat cgaacaggac tacgacacag cggtcgcgct
     1561 cgccaagagc gagctgaacc ccgtctcggc cttcatgacc gggaagatcc ggatcaacgg
     1621 cagcatgatg ctcctgatgc agctccaggg ggccctgtcc gagctcgcca aggagatgca
     1681 ggagatcgac gtcgactact gaggaggcga cggccttccg gcccacccga cgggcgacgt
     1741 gccatcccat cgagggcccc aggacgtacc tgtgaggggt tcgcagaacc tcgtttgacg
     1801 ggctcgaccc ggcgagcgga gaatctacgg ccgtgccccg tgttcggggc gctctcgatt
     1861 gggaggggga cgcatgcgct catggcgagc gaagatgctg gtcctcatcg gtgtcctgtc
     1921 gatcgtcgcc gcggcctgcg gcaacgacga gggggatgag accggcggtg ggggcggcac
     1981 cgccaccggc ggtggcgccg ccgaatgcaa cgcggatctg aaggtggggg tagctttcga
     2041 catcggcggg ctcggggaca agtcgttcaa cgactccgcc aacgccgggc tgcagcaggc
     2101 gatcgcggac ggcctcgtgt gcgaggacaa cgcgatcctc aacgaagcca actcgaccgg
     2161 ctcggaccgg gatcagaacg tccaggccct ggccgacgcc ggcatgaacc tggtcgtcgg
     2221 ggtggggttc gcgttctcgc cggggatcaa cacgatcgcc ccggactacc cggatgtgga
     2281 cttcatgatc gtggacgggt tcgcgacgtg cggaacggcg tgcggtctca ccaacgatgc
     2341 cgaggcgatc ccgaacgtgg ccgactacac cttcaaggag caggagggat cgttcctggt
     2401 gggtgcggcg gccgtcctca agtgccagtg caagacgatc gggttcctcg gtggccagca
     2461 gggcaccggc ctgatcgaga agttcgaggc cggctacgag gcgggcgccc accagatcga
     2521 tccggatgtc gagatcctcg tcgagtacat cggtgacagc accgaggcct tcaacgaccc
     2581 ggtcaagggc gaggaactct cgacgaagat gtacgaccag ggtgccgaga tcatctacca
     2641 cgcgtcgggc gcgtccggag ccgggctgtt caacgccgcc gtcgaggctc agaagctagc
     2701 gatcggcgtg gactcggacc agtacctgac cgcgagcccc gagcagcagc cactgatcct
     2761 gacgtcgatg atcaagcggg tggacgtcgc ggtgtacaac gcgatcgagg cggagggcaa
     2821 cgatacgttc ggcggcagtc aggtcttcgg catggcagag gatggcgtcg actatgcgac
     2881 ctcgaacccg gaggaactga cggccgacat caccgagcaa ctggacgagt tcaagcagca
     2941 gatcatcgac gggacgatcg tcgtgcccga cacgccggaa ggcgcctagg gcccggcgag
     3001 ggacaccgtg acgatcggtg cgggggcggg gagcaacggc tccccgcccc tcgtcgtgct
     3061 cgagcacatc accaagcgct tccctggggt ggtcgccaac gacgaggtcg atctggagct
     3121 ccggtcgggt gaggtccatg ccctgatcgg ggagaacggc gccggcaagt cgacgctgat
     3181 gcgggtcctg tacggcatgt atccctcgga cgggggccgc atcctcgtgc gtggggacga
     3241 ggtcaaggtc gcatcgccga gggatgcgat cgggctcggg atcggcatgg tccaccagca
     3301 cttcgtcctg gtcgatccct tcacggtcac cgagaacatc atcctggggg aggagggcgg
     3361 cgagcgcctc gacatggacg cggcgcaaca gcgtgtctcc gatctcgccg agacctacgc
     3421 gttccgcgtc gaccc
//
LOCUS       DATBRK010000458         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1286443, whole genome shotgun sequence.
ACCESSION   DATBRK010000458 DATBRK010000000
VERSION     DATBRK010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286443"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VIH70_13330"
     CDS             complement(<1..746)
                     /locus_tag="VIH70_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012185044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY8117390.1"
                     /translation="MSKVRLAIVGAGNCASSLVQGLEYYKDADPTDTVPGLMHVDLGG
                     YHIRDVELVAAFDVDAKKVGTDLAEAIVSEPNNTIRFAEVPPTGVTVERGPTFDGLGR
                     YYRETIAESELPPVDVAKALREVQADVVVSYLPVGSEQAAKHYAQAALDAHVAFVNCL
                     PVFIASDKAWAQKFVEAGVPIVGDDIKSQVGATIIHRVLTKLFEDRGVKLNRTYQLNF
                     GGNMDFMNMLERERLTSKKVSKTQSVQSQMR"
     gene            complement(743..1327)
                     /locus_tag="VIH70_13335"
     CDS             complement(743..1327)
                     /locus_tag="VIH70_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY8117391.1"
                     /translation="MLELAILGLLKERSMHGYQLSKRLTDSLGGFWRVSYGSLYPSLK
                     RLERQGAVEQIFDKQEVGRRKNVYRITEKGEQLFRELLEESGPESSGEDNRFRVRMAF
                     FKYLAPETRIRLLERRRAFLEERLSTIQTSLATRSGDMYTTSLMQHGRESTEQDIAWL
                     NGLITAERRAAETTSGGSKRRSTGRALRRKERAT"
     gene            complement(1424..>2530)
                     /locus_tag="VIH70_13340"
     CDS             complement(1424..>2530)
                     /locus_tag="VIH70_13340"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8117392.1"
                     /translation="YLTSRHFSVAQAGLAVAMYGIGGFAAQILGGLLTDRIGRRNTIG
                     FSMLGAAALTLVLWQATTLPVIYPVMALLALVAELHRPAATALITDLVPSEGRVAAFA
                     AFRLAINIGWACGLALGGLLADRSFAYLFIGDAATSAAFGVISLVALPHGVRTSRHEE
                     RHLRGATRTILADRGFLLFLGSVVIGAAIYMQNVSTFALHVRDSGYSNAVYGGLQALN
                     GAIVVFLELPIAAWTGRRSRTGMVALGSVLIGMAFASLAVATAVAAMVAMVFVWTLGE
                     IVASPPASAFVADRSPDHARGRYQASLGMMFALGGVIGPIIGTVVYEHSPVALWIGCG
                     VAGLISAALALAAGRYPAPAFPARLDETVDAPGT"
BASE COUNT          401 a          882 c          862 g          385 t
ORIGIN      
        1 cgcatctggg actggacgga ctgggtcttc gagaccttct tggacgtcag gcgctcgcgc
       61 tcgagcatgt tcatgaagtc catgttgccg ccgaagttca gctggtaggt cctgttcagc
      121 ttcaccccgc ggtcctcgaa cagcttggtc agcacccggt ggatgatcgt ggcgccgacc
      181 tggctcttga tgtcgtcgcc gacgatcggc accccggcct cgacgaactt ctgtgcccat
      241 gccttgtccg aggcgatgaa caccgggagg cagttgacga acgcgacgtg ggcgtcgagc
      301 gctgcctgcg cgtagtgctt ggccgcctgc tcggacccca ccggcaggta cgacacgacc
      361 acgtcggcct gcacctcgcg gagcgccttc gcgacgtcga cggggggcag ctcggactcg
      421 gcgatcgtct cccggtagta ccggccgagg ccgtcgaacg tcggcccccg ctccacggtc
      481 acgccggtcg gtggcacctc tgcgaaccgg atcgtgttgt tgggttcgga gacgatggcc
      541 tccgcgaggt cggtcccgac cttcttggcg tccacgtcga acgccgcgac gagctcgacg
      601 tctcggatgt ggtagccgcc gaggtcgacg tgcatcagcc cggggacggt gtcggtcgga
      661 tccgcgtcct tgtagtactc gagtccctgg acgagcgacg aggcgcagtt gcctgcaccg
      721 acgatcgcga gtcgaacctt gctcatgttg cgcgctcctt tctgcgaagc gcacggccgg
      781 tgctccggcg cttcgagccc cctgacgtcg tctccgcggc gcggcgctcg gccgtgatga
      841 ggccgttcaa ccacgcgatg tcctgctcgg tgctctcccg accgtgctgc atgagcgacg
      901 tcgtgtacat gtcgccgctc ctggtcgcga gcgaggtctg gatcgtcgac agccgctcct
      961 cgaggaaggc ccgacggcgt tcgagcaggc ggatccgcgt ctcgggcgcc aggtacttga
     1021 agaacgccat gcgaacgcgg aagcggttgt cctcgccgga ggactcgggc ccggactcct
     1081 cgagcagctc tcggaacagc tgctcaccct tctcggtgat gcggtacacg ttcttgcgcc
     1141 ggccgacctc ctgcttgtcg aagatctgct ccacggcgcc ctggcgctcc aggcgcttga
     1201 gcgaggggta cagcgagccg tacgagacgc gccagaaccc gccgagcgag tcggtgaggc
     1261 gcttcgacag ctggtacccg tgcatggacc gctccttgag cagtccgagg atcgcgagct
     1321 cgagcactcc ggttccttcc tcgccctcgt gccaccccgg gatcgggcgt cgacacgagt
     1381 gccgatgtat cgagacagta tatcgcttcg atatatctac ccgctacgtc ccgggagcgt
     1441 caaccgtctc gtccagcctg gccgggaagg cgggggccgg gtagcggccc gcggccaatg
     1501 cgagcgcggc cgagatcaga ccggcgaccc cgcacccgat ccacagggcg accgggctgt
     1561 gttcgtagac gacggtcccg atgatcgggc cgatgacgcc gcccagcgcg aacatcatcc
     1621 cgagcgaggc ctggtatcgg ccccgcgcgt ggtcgggcga gcggtcggcc acgaacgcgc
     1681 tcgccggcgg cgaggcgacg atctcgccga gcgtccagac gaagaccatc gccaccatcg
     1741 cggcgaccgc cgtcgcgacg gcgagcgagg cgaaggccat cccgatgagc actgagccga
     1801 gcgcgaccat ccccgttcgg gatcgccgcc ccgtccacgc cgcgatcggg agctcgagga
     1861 agacgacgat cgcgccgttc agcgcctgca acccgccgta gacggcgttc gagtaccccg
     1921 agtcgcgaac gtggagggcg aaggtcgaca cgttctgcat gtagatcgcg gccccgatca
     1981 cgaccgaccc caggaacagg aggaagcccc tgtccgcgag gatcgtccgg gtcgcgcccc
     2041 tgaggtgccg ctcctcgtgg cgcgaggtcc gcacgccgtg cggcagcgcg acgagcgaga
     2101 tcaccccgaa ggcggccgag gtcgccgcgt caccgatgaa caggtaagcg aacgagcggt
     2161 cggcgagcag gccgcccagc gccaacccgc acgcccaccc gatgttgatc gccaaccgga
     2221 acgccgcgaa cgcggccacg cgtccctcgc tcgggacgag atcggtgatc agggccgtcg
     2281 cggccgggcg gtgcagctcg gcgacgagcg cgagcagcgc catcacgggg tagatcacgg
     2341 gcagcgtcgt cgcctgccag agcaccagcg tgagcgccgc ggcgccgagc atcgagaacc
     2401 cgatcgtgtt gcgccggccg atccggtccg tgagcaggcc gccgaggatc tgcgccgcga
     2461 agccaccgat cccgtacatc gccacggcga ggcccgcctg cgccacggag aagtgccgcg
     2521 atgtcaggta
//
LOCUS       DATBRK010000459         6551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2725473, whole genome shotgun sequence.
ACCESSION   DATBRK010000459 DATBRK010000000
VERSION     DATBRK010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2725473"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VIH70_13345"
     CDS             <1..805
                     /locus_tag="VIH70_13345"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8117393.1"
                     /translation="IAMRFGFTGPNLCTVSACSSSNHAIGEALRYIRDGYMDVCVAGG
                     SEAATLPLTVAAFAQMTALTRNPDPETASRPFDRARDGFVLSEGACTLILESETHANA
                     RGARVYCEVAGYGASDDAFHITAPDPKGSGAALAMGWALRDAGEEPEAVTYVNAHGTS
                     TQLNDAAETAAIKATLGEDVAHRIAVSSTKSMTGHMLGAAGAVEGAICALAIANAVVP
                     PTIHYETPDPDCDLDYVPNEARELDVRLAMSNSFGFGGHNAVVAFRRAS"
     gene            complement(836..1240)
                     /gene="ndk"
                     /locus_tag="VIH70_13350"
     CDS             complement(836..1240)
                     /gene="ndk"
                     /locus_tag="VIH70_13350"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY8117394.1"
                     /translation="MTVESTLLVVKPDGVRRGLVGEVLRRAEDKGLRVAEIRSMTIDR
                     ALAEEHYGEHRDKPFFGELVGFITSGPVVVARLEGEGAIDVWRKMMGPTDPATAPPGT
                     IRGDYGLIITENLVHGSDSPDSAERELKLFFG"
     gene            complement(1242..2528)
                     /locus_tag="VIH70_13355"
     CDS             complement(1242..2528)
                     /locus_tag="VIH70_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY8117395.1"
                     /translation="MRFADADAELGLRRPEHLPKPDLDRMRALATLLAEPQLTYPTIH
                     VTGTNGKTTTARLASAVACAHGLTTGLFTSPHLDSVTERLQVCGEPMAEETFGEEFEH
                     LLAYLRTVDEQVGRVTYFEALTALAYLSFADAPVALGVFEVGMGGTWDATNLVSGDVA
                     VICPIGLDHPELGSTIAEVTREKAGIIKQGKVAVVREQEAEALEVIERRCEEQIATLL
                     LEGRDWEVAELTPAVGGQALAIRGLHATYDDAHLPIYGEHAAHSASAAVVAVEALLER
                     ALDPDGVRLALGAATSPGRLEVVARRPLVVLDGAHNPAAARALAEALPDTFTWDRLHL
                     VLGVFADKDLEGIVAPLAPLADAGYATAVDSVRARPPREVADAMAVHGIATEPFPSVP
                     DALDAAARAASDGDLILVTGSLYTVADARRALLEDP"
     gene            complement(2532..5069)
                     /gene="valS"
                     /locus_tag="VIH70_13360"
     CDS             complement(2532..5069)
                     /gene="valS"
                     /locus_tag="VIH70_13360"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY8117396.1"
                     /translation="MNVPEKPTLEGLEAKWGERWETDGTYRFDRSKTRDQVFAIDTPP
                     PTASGTLHVGSVCSYTHTDLIARFRRMRGKILFYPMGWDDNGLPTERRVQNFFGVRCD
                     PSVPYDPAFDLDALDRSGDEPVAVSRPNFIDLCERLTAEDEKAFEGLFRVLGLSVDWS
                     QTYRTIDVRSRRVSQRVFLRLLRRGIAYQAEAPTMWDVDFRTAVAQAEIRDREVDGRY
                     YRIRFDLTDGSGSVEVETSRPELMPACVALVVNPSDDRYRSLVGTTAVTPLFAAEVPI
                     VAHELADPEKGTGVAMICTFGDVTDVTWWRELKLPSRVVVRRDGTIAPPRWGEPGWES
                     RDVESARRAQEEIAGLSSKRARARIAELLGASGALLGEPTQVRHVVKFYENGQRPLEV
                     VSSRQWFVKTIELRDRLLERGRELRWHPPHMGARYASWVEGLNGDWGISRQRFFGVPI
                     PLWYPLDHDGVPDHDNPLVPGEDRLPVDPSSDVPSGYSEDRRGKPDGFVGDPDVMDTW
                     ATSSQTPEIAGGWEEDEDLFGRVFPMDLRPQAHDIIRTWLFYTILKAELIHGKLPWKD
                     AAISGFILDPDRKKMSKSKGNVVTPIGLLEEYGADAVRYWAASARLGLDAAFKEEQIK
                     IGRRLAIKILNASRFTLSLEGAETEIAVALDRSMLVGLADVVGQVTAALEDYEHARAL
                     EIVERFFWGFTDDYLELVKGRAYGGHGPEAAGSAIASLRFALDVLLRLFAPFLPYATE
                     EVWSWWREGSIHRASWPSTAGFPAGGEPVVYEVAAWTLGEIRKAKALARRSLRTDVMR
                     LVVRDTPERLKILRGVERDIAEAGKVRSFEYVEAAEPSVEVDLAPQD"
     gene            5167..>6551
                     /locus_tag="VIH70_13365"
     CDS             5167..>6551
                     /locus_tag="VIH70_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8117397.1"
                     /translation="MKTCAACGGENSDGARFCSACGSSLVPSCPTCGAEVPLEARFCP
                     ACGSALETLEPVPADQERRLVTILFADVTSSTSLGERLDPERLQEVLAAYFGAMREEI
                     EAEGGTVEKFIGDAVMAAFGVPTAHEDDPSRALRAALRMRDRLAEVNADLDRRFGITL
                     QIRTGVNTGEVLAATNPRPGDPMVTGDAVNVAARLEQGADPGGIVVSERTARAARGFR
                     FHQLEERELRGKERAVAALLLEERKPGAPERGVPGLRAPMVGRDRELELLRSLYQRSA
                     EEGQPNLVTIYGDPGVGKSRLVAEFVGWAEGVDPAPTIVRGRCLPYGDGVTYWPLAEI
                     LKGLSHIRDTDPTEVALERVRRLGDDLITGDVAAHPAKATAALAYSIGVEDPAFGFGS
                     MEPREVGLKIHGAWRSLFSALANRAPVVAVIDDIHWADQALLDLLEELADRVVGPAFF
                     LCPSRPELTERR"
BASE COUNT          964 a         2350 c         2270 g          967 t
ORIGIN      
        1 catcgcgatg cggttcggct tcaccggccc gaacctgtgc acggtctcgg cctgttcgtc
       61 gtcgaaccac gcgatcggcg aggcgctgcg gtacatccgg gacggctaca tggacgtctg
      121 tgtcgccggc ggttcggagg cggcaaccct ccccctcacg gtcgccgcgt tcgcacagat
      181 gacggcgctc acgaggaacc ccgatcccga gaccgcgtcg cgaccgttcg atcgggcgcg
      241 ggacgggttc gtgctgtccg agggcgcctg cacgctgatc ctcgagtccg agacgcacgc
      301 gaacgcccgc ggcgcccggg tgtactgcga ggtcgccggc tacggagcgt cggacgacgc
      361 gttccacatc acggcccctg acccgaaggg ctcgggcgcg gccctggcga tgggttgggc
      421 gctgcgcgac gcgggcgagg agccggaggc cgtcacgtac gtcaacgcgc acgggacctc
      481 gacgcagctg aacgacgcgg cggagacggc cgcgatcaag gccaccctcg gcgaggacgt
      541 cgcccaccgc atcgcggtct cctcgacgaa gtcgatgacg ggacacatgc ttggcgcggc
      601 gggggcggtg gagggcgcga tctgcgcgct cgcgatcgct aacgccgtcg tgccgccgac
      661 gatccactac gagacgcccg acccggactg cgacctcgac tacgtgccga acgaggcgcg
      721 cgagctggac gttcgtctcg cgatgtcgaa ctcgttcggg ttcggcggcc acaacgccgt
      781 cgttgcgttc cgcagggcct cgtagccacc gagacccgac gcctcgccga acgcgtcagc
      841 cgaagaagag cttgagctcg cgctcggccg agtcaggcga gtcgctgccg tgcacgaggt
      901 tctcggtgat gatcagtccg taatcgcctc ggatcgtgcc gggcggagcc gtcgccggat
      961 ccgtcggacc catcatcttc ctccagacgt cgatcgcgcc ttccccttcc aggcgtgcga
     1021 ccacgaccgg ccccgaggtg atgaagccga cgagctcgcc gaagaagggc ttgtcgcggt
     1081 gctccccgta gtgctcttcc gcgagcgcgc gatcgatcgt catcgaacgg atctcggcga
     1141 cccgcaggcc cttgtcctcg gcccgccgga ggacctcgcc gacgagcccg cggcgtacac
     1201 cgtcgggttt cacgacgagc agcgtcgatt ccaccgtcat ctcaggggtc ctccaggagc
     1261 gcccggcggg cgtccgcgac agtgtacaga gagcccgtca ccaggatgag gtcgccgtcg
     1321 gaggcggccc gcgccgccgc gtccagcgcg tcggggaccg acgggaaggg ttcggtggcg
     1381 atgccgtgga cggccatcgc gtccgccacc tctcgcggcg gtcgtgcccg aacgctgtcg
     1441 accgccgtcg cgtatccggc gtcggcgagc ggtgcgagcg gagccacgat gccctcgaga
     1501 tccttgtcgg cgaagacgcc aagcacgagg tgcagccgat cccacgtgaa cgtgtcgggc
     1561 aacgcctcgg cgagcgcccg tgccgcggcg gggttgtggg cgccgtcaag gaccaccagc
     1621 ggtcgccgcg ccacaacctc cagccggcct ggcgaggtgg ccgcgccgag cgcgagccgc
     1681 acgccgtcag ggtccagggc gcgctccagc agtgcctcga ccgcgacgac ggcggccgag
     1741 gcgctgtggg ccgcgtgctc cccgtagatc ggcaggtgcg cgtcgtcgta cgtcgcatgg
     1801 agcccccgga tcgcgagcgc ctgtcccccg accgccggcg tgagttcggc gacctcccag
     1861 tcgcgcccct cgagcaggag cgtggcgatc tgctcctcgc accgtcgctc gatcacctcg
     1921 agcgcctccg cctcctgctc gcggacgacc gcgaccttgc cctgcttgat gatgccggcc
     1981 ttctcccgcg tcacctcggc gatcgtcgaa ccgagctccg ggtgatcgag cccgatcggg
     2041 cagatcaccg cgacgtcgcc ggagacgagg ttggtcgcgt cccatgtccc ccccatcccc
     2101 acctcgaaca cgccgagcgc taccggcgcg tcggcgaacg agaggtacgc aagcgccgtg
     2161 agagcttcga agtacgtgac gcggccgacc tgctcgtcga cggtccgcag atacgcgagc
     2221 agatgttcga actcttcgcc gaacgtctcc tctgccatcg gctcaccgca gacctggagc
     2281 cgctccgtca ccgagtcgag gtggggcgag gtgaacagcc cggtcgtgag cccgtgggcg
     2341 cacgcgaccg ccgaggcgag gcgggcggtg gtcgtcttcc cgttggtccc cgtgacgtgg
     2401 atcgtcgggt aggtcagctg tggctcggcc agcaaggtgg cgagcgcgcg catccggtcg
     2461 agatcgggct tgggtaggtg ttcgggccgg cggagcccga gctcggcatc cgcgtcggcg
     2521 aaccgcacgg ttcagtcctg cggcgccagg tcgacctcga ccgagggctc ggcggcttcg
     2581 acgtactcga acgaacggac cttgccggcc tcggcgatgt cccgctccac cccgcgcagg
     2641 atcttcagac gctcgggcgt gtcccggacg acgaggcgca tgacatcggt gcggagcgag
     2701 cgcctcgcga gcgccttcgc cttgcggatc tcgccgagcg tccacgcagc cacctcgtag
     2761 acgaccggct cgccaccggc gggaaagcca gcggtcgacg gccagctcgc gcggtggatc
     2821 gagccctccc gccaccacga ccatacttcc tcggtggcgt agggcaggaa cggggcgaac
     2881 aggcggagca ggacgtcgag ggcgaaccgg agcgaggcga tcgccgaccc ggcggcctcc
     2941 ggaccgtgac cgccgtacgc ccgacccttg acgagctcga ggtagtcgtc ggtgaatccc
     3001 cagaagaagc gttcgacgat ctcgagcgcg cgcgcgtgct catagtcctc gagcgcggcc
     3061 gtcacctgcc ccaccacgtc tgcgagcccc acgagcatcg accggtcgag cgcaacggcg
     3121 atctcggtct ccgcgccctc gagcgacagg gtgaagcgcg aggcgttcag gatcttgatc
     3181 gcgagccgtc gcccgatctt gatctgctcc tccttgaagg cggcgtccag accgagcctc
     3241 gcgctcgcgg cccagtatcg aacggcgtcc gccccgtact cctcgagcag accgatcggc
     3301 gtcaccacgt tccccttgga cttcgacatc ttcttccggt cggggtcgag gatgaagccg
     3361 ctgatcgcgg cgtctttcca cggcagtttc ccgtggatca gctcggcctt caggatcgtg
     3421 tagaagagcc aggtcctgat gatgtcgtgg gcctggggac gaaggtccat cgggaagacc
     3481 cgaccgaaga ggtcctcgtc ctcctcccac cccccggcga tctccggcgt ctgggaggag
     3541 gtggcccacg tgtccatgac atcggggtcc ccgacgaacc cgtccggctt gccgcgccga
     3601 tcctccgagt acccgctcgg gacgtcggag ctcgggtcga cggggagccg gtcctcgccc
     3661 gggaccagcg ggttgtcgtg atccggcacg ccgtcgtggt cgagcgggta ccacagcggg
     3721 atcggaacgc cgaagaaccg ctgccggctg atgccccagt ccccgttcaa gccctcgacc
     3781 cacgacgcgt accgtgcccc catgtgcggc gggtgccacc gcaactcgcg cccacgctcg
     3841 aggaggcggt cgcggagctc gatcgtcttc acgaaccact gccggctgga gaccacctcc
     3901 agcggtcgct gtccgttctc gtagaacttc acgacgtggc gcacctgcgt cggctcgccg
     3961 aggagggccc ccgaggcgcc gaggagctcg gcgatccgcg cgcgagccct cttcgaggag
     4021 agcccggcga tctcctcctg cgcccggcga gcggactcga cgtcccgcga ctcccatccc
     4081 ggctcccccc accgaggcgg cgcgatcgtg ccgtctcgtc gaaccacgac gcgggacggc
     4141 agcttcagct ctcgccacca tgtgacgtcg gtgacgtcgc cgaaggtgca gatcatcgcg
     4201 acgccggtcc ccttctccgg atccgccagc tcgtgcgcga cgatcgggac ctcggcggcg
     4261 aacaacggtg tcacggccgt cgtgccgacg agcgaccggt accggtcgtc ggatggattc
     4321 acgacgagcg cgacgcaggc cggcatgagc tcgggcctcg acgtttcgac ctccaccgat
     4381 cccgagccgt cggtgaggtc gaaccggatg cggtagtagc ggccgtccac ctctcgatcc
     4441 cggatctccg cctgcgcgac ggccgtccgg aagtcgacgt cccacatcgt cggcgcctcg
     4501 gcctggtacg cgatcccacg acgaaggagc cggaggaaca cgcgctgcga gacgcgtcgc
     4561 gagcgcacat cgatcgttcg gtacgtctgg ctccagtcaa ccgagaggcc gagcacgcgg
     4621 aacagtccct cgaacgcctt ctcatcctcg gcggtcagtc gttcgcacag atcgatgaag
     4681 ttcggtcgcg agacggcgac cggctcgtca ccggaacggt cgagcgcgtc gagatcgaag
     4741 gccgggtcgt acggaaccga ggggtcgcac cgaaccccga agaagttctg gacgcggcgc
     4801 tctgtcggca gaccgttgtc atcccaaccc atcgggtaga agaggatctt gccccgcatg
     4861 cgccggaatc gggcgatgag gtcggtatgc gtatacgagc agacagaccc gacgtgcagg
     4921 gtcccgctcg cggtcggcgg cggggtatcg atcgcgaaga cctggtcccg cgtcttcgaa
     4981 cggtcgaacc ggtaggtgcc gtcggtttcc caccgctcgc cccacttcgc ttcgaggccc
     5041 tccagcgtcg gcttctcggg cacgttcatg cgcgggagtc taagcgatgc ccgcatcgcc
     5101 tccggtcgcc cggcgggccg cgcgccgacg cgttcagcgt cggtacccgg tcgtacactc
     5161 gcgctcgtga agacctgcgc cgcatgcggc ggcgagaact ccgacggcgc ccgcttctgt
     5221 tcggcgtgcg ggtcgtcgct cgttccgtcg tgtcccacct gcggagccga ggtgccgctc
     5281 gaggcccgct tctgtcccgc gtgcggctcc gcgctcgaga cgctcgaacc ggtcccggcg
     5341 gaccaggaac ggcgcctcgt gacgatcctg ttcgcggacg tgacgagctc gacgagcctc
     5401 ggcgagcgcc tggatcccga gcggctccag gaggtgctcg cggcctactt cggggcgatg
     5461 cgcgaggaga tcgaggcgga ggggggcacg gtcgagaagt tcatcggcga tgccgtcatg
     5521 gcggcgttcg gcgtaccgac ggcgcacgag gacgacccct cgagggcgct ccgcgcggcg
     5581 ctccgcatgc gcgaccgcct cgccgaggtc aacgcggacc tcgaccgccg cttcggcatc
     5641 acgctacaga tccggaccgg cgtgaacacc ggcgaggtcc tcgccgcgac gaacccccga
     5701 cccggcgacc cgatggtgac cggcgacgcg gtgaacgtcg ccgctcggct cgagcagggt
     5761 gccgaccccg gcgggatcgt ggtgtccgaa cgcacggcgc gggcggcccg cggcttccgg
     5821 ttccatcagc tcgaagagcg agagctccgc ggcaaggagc gggccgtcgc cgccctcctg
     5881 cttgaggagc ggaaacccgg tgcgcccgag cgcggcgtcc ccgggcttcg cgcgccgatg
     5941 gtcgggcgcg accgcgagct cgagctcctc cgcagcctgt atcagcgctc cgccgaggag
     6001 ggccagccga acctggtgac gatctacggg gacccgggcg tcggcaagag ccggctcgtc
     6061 gccgagttcg tcgggtgggc ggagggggtc gatccggcgc cgacgatcgt gcggggtcga
     6121 tgcctgccct acggcgacgg ggtcacctat tggcccctgg ccgagatcct caagggactt
     6181 tcgcacatcc gcgacaccga cccgaccgag gtcgcgctcg agcgggtccg gcggctcggc
     6241 gacgacctca tcaccggcga cgtcgccgcg cacccggcca aggcgacggc tgcgctcgcg
     6301 tacagcatcg gcgtcgagga tcccgcgttc ggcttcggct cgatggagcc gcgcgaggtc
     6361 ggcctcaaga tccacggggc gtggcgttcg ctcttctccg ccctcgcgaa ccgggcgccg
     6421 gtcgtcgcgg tgatcgacga catccactgg gccgaccagg cgctgctcga tctgctggag
     6481 gagctcgccg accgcgtcgt ggggccggcg ttcttcctgt gtccgtcacg cccggagctg
     6541 accgagcggc g
//
LOCUS       DATBRK010000460         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_403372, whole genome shotgun sequence.
ACCESSION   DATBRK010000460 DATBRK010000000
VERSION     DATBRK010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403372"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VIH70_13370"
     CDS             complement(<1..473)
                     /locus_tag="VIH70_13370"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEY8117398.1"
                     /translation="MANPTSKQRLTKAERKEEARRQRVELQRKMARSRRNRRVAIGVV
                     VLLIAGVAAFALTRPKQAQASPSDLLASVDQARQTAGCQPVEDVGPYDPKSQDRAHVV
                     ARVPLSTYPSVPPASGPHNAIPYGAGVYSTPPPVDRVIHSLEHGAAIVWYAPDASG"
     gene            664..2085
                     /locus_tag="VIH70_13375"
     CDS             664..2085
                     /locus_tag="VIH70_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEY8117399.1"
                     /translation="MARTDTGSGNVVRVGTPPVGGLDKFFKISERGSTVRIEIIAGLT
                     TWLTMAYILFVNPSILGFAGVPDLEPLGLPFAQVLTVTALVAGVMTILMGVVANYPFA
                     LAAGLGLNAFVAFTLVAANGLSFPQAMGVIVVEGVVITILVLTGVREAVLNAIPMDLK
                     RAIGIGIGAFIALIGLVNAGVVTNTGATPLALAGELTTWPIGIFVVGLGLSAALVARR
                     VKGALLIGILVSTVLAVIVNEAKDLSIWGNGIAAIPDKVVDTPDFGLVGAFSFDFFSV
                     LGTATALAVVLSVMLSDFFDTMGTVIGLGGEMKMLDSNGRLPGIKRVLLIDSLAAAAG
                     GAASASSNTTYIESASGISEGGRTGLVAVVVGVLFLLAMFFSPIAGVIPPEATAPALV
                     LVGYFMMVLVKDISWSDPGIGIPALLTIVMMPFTYSITNGVGAGFLSYVVIAVLRGRW
                     RDVHPLLYVVAAIFAWYFVHGVV"
     gene            complement(2107..>3173)
                     /locus_tag="VIH70_13380"
     CDS             complement(2107..>3173)
                     /locus_tag="VIH70_13380"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF009810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY8117400.1"
                     /translation="RGDLVIAETRHGFVCANAGVDASNVEEGFLTLLPEDPDASAERL
                     RRDLSERLGVEVAVVITDTFGRTWRRGLVNVSIGCAGIPALVDLRGTLDHHGRRLEAT
                     IVALADEVAAASGLAMGKTARVPVAIVRGVEFDPASVPASEMVRPPEEDLFRTSPLLS
                     ISERRTIRAFGDGEVAHEAVEEAVRAACTAPAPHHTRPWSFTAIWTSAARRRLLRAIA
                     DAWREDLRADATPEAMIERRIARSDAVLGAAPVLIVPWIRFRGAHPYADAERSHAEQE
                     MFLLSGGAAIQNLLLALSAQGLASSWISSTLFCQQESRAALGMSDEWFALGTVAVGPM
                     PAGAASPRPPLDLSEHLRTE"
BASE COUNT          413 a         1084 c         1156 g          520 t
ORIGIN      
        1 cccgacgcgt cgggcgcgta ccagacgatc gccgcgccgt gctcgagcga gtggatcacg
       61 cggtcgaccg gcggcggcgt gctgtagacg ccggcgccat acgggatcgc gttgtgcggt
      121 ccggacgcag gcggcacgga cgggtacgtc gagagtggca cccgagcgac gacatgagcc
      181 cgatcctggc tcttcggatc gtacggaccg acgtcctcaa ccggctggca gccggcggtc
      241 tgcctcgcct ggtcgacgga ggccaggagg tccgacggcg aggcctgggc ctgcttcggc
      301 ctggtgaggg cgaacgcggc gacccccgcg atcaggagga cgacgacgcc gatcgcgacc
      361 cggcggttgc ggcggctcct cgccatcttg cgctggagct cgacacgctg acgcctggcc
      421 tcctccttgc gttcggcctt cgtcagacgc tgcttcgagg tcgggttggc catgtctggt
      481 tccgcccgca ggcggtcacc caggatagcg aggaagcgaa cggttcggcc ggtcacatct
      541 gccgtccgat cggcgggtcc gttctgagcc cggagccttg cggcggccca cccgccttcc
      601 tatgcttcgg cctacttccc cacggcgctg gccggcgttt cgtgccggcc ggaaggggga
      661 ggcatggcgc gcacggacac gggcagcggg aacgtggttc gcgtagggac gccgccggtc
      721 ggcgggctcg acaagttctt caagatctcg gagcgcggtt ccaccgtgcg gatcgagatc
      781 atcgccggcc tcacgacgtg gctgacgatg gcctacatcc tgttcgtgaa cccgtccatc
      841 ctcggcttcg cgggggtccc cgacctggag ccgctcgggc ttccgttcgc gcaggtcctg
      901 accgtgacgg cgctcgtcgc cggtgtgatg acgatcctga tgggggtggt cgccaactac
      961 ccgttcgcgc tcgccgcggg gctggggctg aacgccttcg tcgccttcac gctcgtcgcc
     1021 gcgaacgggc tctcgttccc gcaggcgatg ggcgtcatcg tcgtcgaggg ggtcgtcatc
     1081 acgatcctcg tcctcaccgg cgtccgcgag gcggtcttga acgcgatccc catggacctc
     1141 aagcgtgcga tcgggatcgg catcggggcg ttcatcgcgc tgatcggcct ggtgaacgcc
     1201 ggggtggtga cgaacacggg cgccacgccg ctcgcgctcg cgggcgagct cacgacgtgg
     1261 ccgatcggca tcttcgtcgt ggggctcggg ttgagcgccg cgctggtggc gcgcagggtc
     1321 aagggggccc tgctgatcgg gatcttggtc tcgacggtgc tggccgtcat cgtgaacgag
     1381 gcaaaggacc tgtcgatctg gggcaacggg atcgcggcga tcccggacaa ggtcgtcgac
     1441 acgccggact tcgggctggt gggcgcgttc tcgttcgact tcttctcggt gctcggtacg
     1501 gcgaccgcgc tcgcggtggt gctctcggtc atgctgtcgg acttcttcga cacgatgggc
     1561 acggtcatcg gcctcggcgg cgagatgaag atgctcgact cgaacgggag gctccccggc
     1621 atcaagcggg tcctgctgat cgactcgctc gcggccgcgg ccggcggcgc cgcgtcggcg
     1681 tcctcgaaca ccacctacat cgagagcgcg tcggggatct cggagggcgg tcgcaccggg
     1741 ctcgtggccg tggtcgtcgg cgtgctgttc ctgctcgcga tgttcttctc gccgatcgcg
     1801 ggtgtgatcc cgccggaggc gacggcgccg gcgctcgtgc tcgtcggcta cttcatgatg
     1861 gtgctcgtca aggacatctc gtggtcggac cccggcatcg ggatcccggc gctgctcacg
     1921 atcgtgatga tgcccttcac gtacagcatc accaacggcg tgggggcggg gttcctctcg
     1981 tacgtcgtga tcgcggtgct tcgcggcagg tggcgggacg tccacccgct cctgtacgtc
     2041 gtggccgcga tcttcgcctg gtatttcgtg cacggggtgg tctaggccga ggggttgcgg
     2101 gcctgctcac tcggtccgca ggtgctcgga gaggtcgagc gggggccgtg gcgacgcggc
     2161 ccccgccggc atcgggccca ccgccacggt tccgagcgcg aaccactcgt cggacatccc
     2221 gagggcggcg cggctctcct gctggcagaa cagcgtcgag gagatccagg acgaggcgag
     2281 cccctgcgcc gagagcgcga ggagcaggtt ctggatcgcc gccccgccgg ataacaggaa
     2341 catctcctgc tcggcgtggg accgttcggc gtcggcgtag gggtgggcgc ctcggaaccg
     2401 gatccacggg acgatcagga cgggcgcggc gccgagcacc gcgtcgctcc ttgcgatccg
     2461 gcgttcgatc atggcctccg gcgtcgcgtc ggctcgcagg tcctcgcgcc aggcgtcggc
     2521 gatcgctcgg agcaaccgcc gcctggccgc cgaggtccag atcgccgtga aggaccacgg
     2581 acgggtgtga tgcggcgccg gcgcggtgca ggcggcacgg acggcctcct ccaccgcctc
     2641 gtgtgccacc tcgccgtccc cgaacgcccg gatcgttcgg cgctccgaga tcgacaggag
     2701 cggggaagtc cgaaacagat cctcctccgg cgggcggacc atctcggacg ccggtaccga
     2761 tgccggatcg aattcgacgc cccggacgat cgccaccggg acccgcgccg tcttgcccat
     2821 ggcgagcccg ctcgcggcgg ccacctcgtc ggcgagcgcc acgatcgtcg cctcgagccg
     2881 gcggccgtgg tggtcgaggg tgccgcggag gtcgacgagc gccgggatgc cggcgcagcc
     2941 gatcgagacg ttcacgagac ctcgccgcca ggtcctgccg aacgtatccg tgatcacgac
     3001 ggcgacctcg acgccgagcc gttcgctcag gtcgcggcgg agccgttccg cggaggcgtc
     3061 cgggtcctcc gggagcagcg tgaggaaccc ctcctcgacg ttcgacgcgt cgacgccggc
     3121 gttcgcgcac acgaagccgt ggcgcgtctc ggcgatcacg aggtcgccgc gcc
//
LOCUS       DATBRK010000461        17086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_135387, whole genome shotgun sequence.
ACCESSION   DATBRK010000461 DATBRK010000000
VERSION     DATBRK010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17086
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135387"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VIH70_13385"
     CDS             complement(<1..661)
                     /locus_tag="VIH70_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY8117401.1"
                     /translation="MDVSTVSADRRRLFLASFLMLFVELVLIRWLGAHIAYLSFFSNF
                     VLLGSFLGIGIGFLAVGRSDWFRRAPMWLGALVFASAVFPVTIDRTGSSLIFFGGFAT
                     RGLPIWISLPVVFAAVAATMAAIAQGVAHLFQRFPPLEAYRLDVLGSVTGIAVFATLS
                     FVGAPPVVWGAVIVGLFVFLLRPSLRIEHAVAIGLLVGAFGMEALAGSVVWSPYYRIN
                     WT"
     gene            957..1127
                     /locus_tag="VIH70_13390"
     CDS             957..1127
                     /locus_tag="VIH70_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117402.1"
                     /translation="MEDLLGLGEDKWWERLLDRWEHVPDRARTLIERGFIGILVVGVF
                     LLLNLLMGLPVG"
     gene            1351..2121
                     /locus_tag="VIH70_13395"
     CDS             1351..2121
                     /locus_tag="VIH70_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017295065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1698 domain-containing protein"
                     /protein_id="HEY8117403.1"
                     /translation="MGTGPPRASELTLETEELQTEIAKVTWYHSIPLGDGIVTPGVDK
                     SSLRLPKIHLPEDLSGKSVLDVGAWDGFFSFEAERRGAERVLATDSFCWGGGGWGTKD
                     GFDLARRALGSRVEDREIDVLELSPQTVGTFDVVLMLGILYHMKHPMLALEKVRSVTQ
                     ERLIMSTFVDMTWWPKPAMAFYEGSEANRDPTNWWGPNPAAVVGMLRAAGFERVEIVD
                     RPTLLRHRLSAQVRARTGIGPPVTTRLWQGAVVCHAWV"
     gene            complement(2143..2916)
                     /locus_tag="VIH70_13400"
     CDS             complement(2143..2916)
                     /locus_tag="VIH70_13400"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY8117404.1"
                     /translation="MPTVARFNVTPVKSTALHHPERIRLDERGAAGDRRFFLVDGSGR
                     RFSGATKAPILPIEATYDEAADRLELRLPNGVVVAGTAEPDGEALEVNFYGRQVAAHV
                     VDGDFSEALSAYAGHEVRLARPDRPGDAIDVRPVTLVSLESVAELARRGEHEGTLDPG
                     RFRMTIEIEGVSAPHEEDTWAARRVRVGGAVIRVDEPVPRCVVTTLDPATGLRDFPTL
                     KVIRDYRGINAEQQLEFGVYADVVEPGEVSVGDPVEPVP"
     gene            3016..3492
                     /locus_tag="VIH70_13405"
     CDS             3016..3492
                     /locus_tag="VIH70_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEY8117405.1"
                     /translation="MATPSQLHADLAELAFLLGDWSGTGEGVWPPGERFDYAEDVTFE
                     FVGDPFLLYAQRSWSLDDGSPIHLERGFLRPAGPGIVELLLAHPIGITEVAVGTVDDG
                     LIELSSTAVSLAPTASPVTELRRRIQGRGDELSFELQMAMEGVELRWHVGSKLTRA"
     gene            complement(3572..5137)
                     /locus_tag="VIH70_13410"
     CDS             complement(3572..5137)
                     /locus_tag="VIH70_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8117406.1"
                     /translation="MTPTEPERLPPGDEPEAELDRLRALLRDLPGMVYIEDADPRIEG
                     PGPFLYVSPGVERILGYTVEEWLADPAGWVERLHPDDVPAMRAAYQAVVPTGAPYSAD
                     YRMFAKDGRIVWIHDEAVLVRDEDGAPLYWQGVMHETVERPAALDRLQEAEQRYRALV
                     EQTPVVTYIDPHRPDHTSAYFSPQVEALLGYTPDELTRDIPLWPELVHPDDRERILGA
                     SLLAEQTNGTYDVEYRMIAKDGRVVWVHDQAALIRDDQGTPKFWQGAWIDLTEQKRAA
                     ELERALQAEREEAAELRAIDELKTTFLQAVSHDLRTPLAAILGLAVTLESQTDLGSDE
                     AHDLASRISMNARKLDRIVSDLLDLDRISRGILEPNLRATDLDELVRRVVIESDLLAE
                     HPTQLDLDPVTIEADAAKIERIFENLLSNAARHTPAGSKVWIRVGPEAGGALLVVEDD
                     GPGVPPEHREGIFQPFGQGAGAPEHSPGVGIGLAVVSRFAELHGGRAWVEEREGGGAS
                     FKVFLPGKRSRGA"
     gene            complement(5196..9623)
                     /locus_tag="VIH70_13415"
     CDS             complement(5196..9623)
                     /locus_tag="VIH70_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8117407.1"
                     /translation="MSLEGFSPAVRAWFESTFETPTAAQAQGWPAIAGGRHSLILAPT
                     GSGKTLAAFLWALDRVMTDPPPPTDRRCRVLYISPLRALAVDIEKNLRTPLVGIGLAA
                     ERLGEHVHEPRVAMRTGDTTAAERRRIQTRPPDVLITTPESLYLMLTSQARDVLRSVR
                     WVIVDEIHAMAGSKRGAHLALSLERLEEIAHEPPQRVGLSATQRPLEEIARFLGGQTP
                     SGPRPVTIVDAGLRKPMEVEVVVPVEDMADLSDVAETPQRMPPMLGPATPGTAPPRAS
                     IWPAIHPQVLELIRGHRSTIVFVNARRLAERLAARLNELAGEELARAHHGSIAREQRL
                     QIEDQLKRGTLKAIVATSSLELGIDMGAVDLVIQVESPGSVARGLQRIGRGGHQVGEP
                     SRGKVFPKFRGDLLEATVVVQRMLRGAIEETHYPRNPLDVVAQQIVAMCAMDEWDVGD
                     LGRVLRRAANFEELSDDLLRNVLDLLAGRYPSDEFAELRPRIVWDRRTDTLRGREGAG
                     RLAIASGGTIPDRGLFGVFTVDGRRVGELDEEMVYESRRGEVFMLGASSWRIEDITLD
                     RVVVSPAPGEPGKMPFWHGDKPGRPLELGRAIGAATRELSSSEHDAAVGRLRTEFGLD
                     ELAAENLTAYLDDQREATGAVPDDRTIVVERFPDEIGDWRVCVLSPFGERVHAPWAMA
                     IEGRLAERYGLDVQVLWSDDGIAIRLPESEERIPVEDLLFDPDEIEELVVARLPGTAL
                     FTTMFREAAARALLLPRRTPGRRTPLWQQRQRGADLLEVASHHPTFPILLEATREVLR
                     DVFDVPGLREVLGDVRARRIHVVPVDTPRSSPFAQSLLFRWVSVYMYEADAPMAERRA
                     AALSLDRELLRELLGAEDLRELIDPVALAELELELQHLAAGRRARNADDVHDLLRRLG
                     HLSTEEVSARSNADPSAWLDDLERDGRAIRMRVAGQDRFVAVEDAGRYRDALGASLPL
                     GVPGVFTEATPAPLEGLVARFARTHGPFGTAEVAERFGIGEDRVAGAVASLEAQGRVV
                     RGEFRPGGLEREWCDADVLRSIRRRSLAALRKEVEPVEAPALGRFVPEWQAATSPRHG
                     PTALVDAIAQLQGASIPASIVESEVLRSRVRGYRPADLDALCASGDVVWMGAGGIGAD
                     DGRIALGFRETLRLAAPPLPETPPAGPVHDAIRDHLRQRGASFWPELLAAAGIPDERI
                     VLAALWDLVWAGEVTNDTIAPMRAFLIRRPTKAARSGAKPRPGALRRAGPPSAAGRWS
                     LVRTLLEPEPSPTEAAHARAMQLLERHGVLTREAVLAEGAPGGFAGVYAVLRALEESG
                     KVRRGYFVDGLGAAQFALPGAVERIRDLRETSREASVLALAAADPAQPYGAALPWPQG
                     TGQGSGRPSRTAGAYVVLADGEPAVFLERGARTLLTFAAGGDGAWIDALASLVKDGRL
                     RRIELSKIDGEPVGGSAFAEDLRTAGFVDGYRGLVLRGS"
     gene            complement(9666..10142)
                     /locus_tag="VIH70_13420"
     CDS             complement(9666..10142)
                     /locus_tag="VIH70_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8117408.1"
                     /translation="MSDEVVTLREITSANEADVRALRVAPDQERFVSSVADSLEDPQA
                     RPWFRAIYAGERPVGFVMLSYDMPPGDPEQPFRYFLWRLLIDAGHQGHGYGRAAVTVV
                     IDQLRGRPGATELVTSVHPGAGSPAPFYRAMGFEPTGDWLEGEEIYQLTLEPDPEA"
     gene            10195..11190
                     /locus_tag="VIH70_13425"
     CDS             10195..11190
                     /locus_tag="VIH70_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY8117409.1"
                     /translation="MQRTRTGIALMLGGFCLVVPALASAQTDPADAEPRDQIVLSGDI
                     LVRRGQEVGEVVVLHGTATIAGVVRGDVVVLDGRIEVTGQVSGAVVSVSGSVGLGPSS
                     RVLGDVLAHERVVAAPGARVDGDVREGVTFTLRAPIEALGAFAPWLAVWCSLLALGVL
                     LLLLGPRAADAVSSAAHGSLWASVGWGFAAFVALPLAGVIGVFSLVWLPLGLGLLLSL
                     FLLYSVGVAWSAFALGRVLWHEPRSRTLAFVIGWAILAAVAAIPFVGGVVWVAGAVFG
                     VGSSTMAIWRARRAAPRAGGRHRQGGKMPVVIPASSDEPEPMIVEREMGEEGAGL"
     gene            11187..11594
                     /locus_tag="VIH70_13430"
     CDS             11187..11594
                     /locus_tag="VIH70_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8117410.1"
                     /translation="MSDTAGLVRKVPMFAELDDDETTWVADLATEFEVPAGFVLIERG
                     QPGSGMFIITDGTVTVELPQGPVELGRGEFVGELPLLADGITRVARVRATTPAKGLAI
                     SRMALSELLQREPRVAVRMLPVLARRLAEAESP"
     gene            complement(11591..12061)
                     /locus_tag="VIH70_13435"
     CDS             complement(11591..12061)
                     /locus_tag="VIH70_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117411.1"
                     /translation="MDQPVEEHAEQGILKTVEAAGDVTVERALVGVVNGRDVSITQAG
                     AGPIAAQGDVRIQQGGCGPVLVGGDLRIHQGGCGPAMVRGNLSIQQGGTQSVLAAGNA
                     TVGHGAFVGLVASPKVTIEEGARVVLGTPGAFAAGAAVGALVAMIAGRRRRRSR"
     gene            12102..13163
                     /locus_tag="VIH70_13440"
     CDS             12102..13163
                     /locus_tag="VIH70_13440"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY8117412.1"
                     /translation="MAQKARTFLLMGSGEFEPWSNEIEAAALDGRTGPVAVLPTASST
                     EGEDVFGRWGRMALEHYATAGIDARLVPVKTREDADREEHARALEGSAMVFFSGGKPQ
                     HLATTIHGTRLWRALEDALDEGAAFAGCSAGALIASQSREQRRERGVRTGWVFGLGLV
                     PHVSFGVHWDKVKVIPGLRSVVMSRTPPGSWFVGIDERTAILGDGQRWRVYGLGGVMV
                     RHAGGTQLHLEGANFETSDQGDRGPADILVDRGLDRRTDRAGAPADVLPLWPAHLRPR
                     EAGAAVGAGDLGRTPGPGLPLMPGGATGLGRSARPVRVLRRDSAHGDARTGRVQGLRS
                     RQGRTGRARVVDDGRLAQR"
     gene            13143..13217
                     /locus_tag="VIH70_13445"
     tRNA            13143..13217
                     /locus_tag="VIH70_13445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13175..13177,aa:Val,seq:tac)
     gene            complement(13231..13698)
                     /locus_tag="VIH70_13450"
     CDS             complement(13231..13698)
                     /locus_tag="VIH70_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117413.1"
                     /translation="MADLQRIVPDTIVDDRVMLILDPADADGFPDSTADLAPRDPHAG
                     IGGQDLAVLGVGERLVGSLLPAAKTCSVPLPFATVRPVHSSASSSRERFPLLSAALGD
                     SPLLGQRPLLAGTAGMATPAHGAQVGEIVRAAGLGRLRHADHEHVIPIDAAKR"
     gene            complement(13885..14400)
                     /locus_tag="VIH70_13455"
     CDS             complement(13885..14400)
                     /locus_tag="VIH70_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117414.1"
                     /translation="MLMLALGTMLVIAAGGLVLTNWRDWPVAAFSVVLGGAFGEGGVH
                     FLRRAVKPVFEIDPTGVTYHPLDDSFSWKTERIPWDRVRKVRIAAPPVQAPLLGEYLV
                     GEYLYIDYEEATVRNWFTRAYDLMAGRRPGDPEPLAHAALALRLSSIPEDELFQLLED
                     LSKRHGFALDR"
     gene            complement(14641..15009)
                     /locus_tag="VIH70_13460"
     CDS             complement(14641..15009)
                     /locus_tag="VIH70_13460"
                     /inference="COORDINATES: protein motif:HMM:NF020366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HEY8117415.1"
                     /translation="MGVIISAPPAARQDGILPNINVVLEPLRLELSTDQYFLASKQTV
                     AGMFAGFELVDQGRTRIDGQPARWMDYRWHSQGTALRQRQAYQVRGDTGYVITFTASP
                     SAFEEHVGSQLIVEESFRIA"
     gene            complement(15470..15973)
                     /locus_tag="VIH70_13465"
     CDS             complement(15470..15973)
                     /locus_tag="VIH70_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117416.1"
                     /translation="MKTLKMVEWTLRRRDRNRRNERIAAGVVGITILVAVLWIVTSGG
                     AFDRSTPAVPGGSETGPTQSSTSEGVTLLLEPRSDGRCCLVTSVNPGESSTIVLCFVE
                     VFDEADRLLSTHLVPPIPPGHRRSSGFEAPPGRQGHGFQGIPLDLSSQHYVSTCRPAA
                     WHGGGPL"
     gene            complement(16727..>17086)
                     /locus_tag="VIH70_13470"
     CDS             complement(16727..>17086)
                     /locus_tag="VIH70_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117417.1"
                     /translation="EPSTGPLREPVPLPPGYDFALVAGISDEQVHAYVSTTSMSDAEA
                     DRGERAVMRYYRHALNDWTIVETDEANLVVKDPDSTSGLEIDARTGVWVMGGRVELEI
                     RQLYCPEDYSCTWTAAG"
BASE COUNT         2576 a         5908 c         6044 g         2558 t
ORIGIN      
        1 gcgtccagtt gatccggtag tagggggacc acaccaccga gccggcgagc gcctccatac
       61 caaacgcgcc gacgaggagc ccgatcgcaa cggcgtgttc gatcctgaga gatggcctga
      121 gcagaaagac gaacagccca acgatcacgg ccccccagac gacgggcggg gctccgacga
      181 acgacaaggt ggcgaacacg gcgatgcctg tcacgctgcc cagcacatcc agtcgatagg
      241 cctccagcgg cgggaatcgc tggaacagat gcgcgacgcc ctgcgcgatc gccgccatgg
      301 tcgcagcgac cgcagcgaag acgaccggca acgagatcca gatcggcagg ccacgggttg
      361 cgaacccgcc gaagaagatg aggctgctgc ccgtgcggtc gatcgtgacc gggaacacgg
      421 ccgacgcgaa aacgagggcc ccgagccaca tgggggcccg ccggaaccag tccgatcggc
      481 cgaccgcgag gaacccgata ccgatgccga ggaacgagcc gaggagcacg aagttcgaga
      541 agaatgagag gtaggcgatg tgcgccccga gccaccggat caggaccagc tccacgaaca
      601 gcatcaggaa gctggccagg aacaatcgcc gccggtcggc cgagaccgtg ctcacgtcca
      661 cgtgtgctcc ttacgcggcc ctcccgatgg cccggaactg cgttcgtgaa gcgggcacgc
      721 ctttgtgcct tcgacagctc caccgcgtca cttgagggcg aagtgctccc tttgagctcg
      781 cgtgctcgcc atcgtttgcg ctaccgacga aggcttcagc gtcgcggcga gaccaccggt
      841 ccgtcgtgcg gcgccggctc tcgtgcccca gtggggggtt gacctccgaa ggactgaagt
      901 actcgagcat cctcgccgat aggcagtagg ggatcggatt cggccgggga ggcatcgtgg
      961 aagatctact cggactgggc gaggacaaat ggtgggagcg gctcttggac cggtgggagc
     1021 acgtgccgga cagagcgaga accttgatcg aacgcggatt catcggcatc ttggtcgtgg
     1081 gcgtgttcct gctgctcaac ctcttgatgg gactgcccgt ggggtaggtt gccccaccga
     1141 ggagtcaaga ggggacggag aaccggaagg tcgggaagcg gagcttcgtt ccattcccca
     1201 accaggctga gctgcgccac gccactcgac atcgatcggg tcgaccggat cggctcgaac
     1261 gacgggtctc catccgccct ggagtcgata cgagaacatg ctgccggcct cgcgtcgcgt
     1321 gcctccgcct cccgtcatac agtggcgctg atgggcacag gtccaccgag ggcatcggag
     1381 ctgaccctgg agaccgagga actgcagacc gagatcgcca aggtcacctg gtatcactcg
     1441 atcccgctcg gggacggcat cgtcacgcca ggggtcgaca aaagctcgct ccgcctgccg
     1501 aagatccatc tgcccgagga cctcagcggg aagagtgtcc tcgacgtcgg cgcctgggat
     1561 gggttcttct ccttcgaggc cgaacgccgc ggcgccgaac gcgtcctcgc gaccgattcc
     1621 ttctgttggg gaggcggtgg gtgggggacc aaggacgggt tcgacctggc acggcgtgcg
     1681 ctgggctcga gggtcgagga ccgcgagatc gacgtgctcg aactatcgcc ccagaccgtc
     1741 ggcacgttcg atgtggtcct gatgctcggg atcctgtacc acatgaagca tccgatgctc
     1801 gccctcgaga aggtgcgaag cgtgacgcag gaacgcctga tcatgagcac gttcgtcgac
     1861 atgacctggt ggcccaagcc cgccatggcg ttctacgagg gctcggaggc caaccgggat
     1921 cccaccaact ggtgggggcc gaaccccgcc gcggtcgttg ggatgcttcg ggccgccggc
     1981 ttcgaacgcg tcgagatcgt cgaccggccc acgctgctcc ggcaccggct ctccgcccag
     2041 gtgcgtgcgc ggaccgggat cggcccgccc gtcacgacga ggctgtggca gggagcggtc
     2101 gtctgtcacg cgtgggtgta gctcgcctcg aaccgacgcg cgtcacggga cgggttcgac
     2161 ggggtcgccc accgagacct cacccggctc gacgacgtcc gcgtagacgc cgaactcgag
     2221 ctgctgctcg gcgttgatgc cccggtagtc tcggatgacc ttgagcgtcg ggaagtcgcg
     2281 gaggccggtt gccgggtcga gcgtcgtcac gacgcaccgc gggaccggct cgtcgacgcg
     2341 gatcaccgcg ccgccgacac ggacgcgccg cgccgcccac gtgtcctcct cgtgcggcgc
     2401 cgagacgccc tcgatctcga tcgtcatccg gaaccggccc gggtcgagcg tgccctcgtg
     2461 ctcgcccctg cgcgcgagct cagccaccga ctcgagcgag acgagcgtga ccggccggac
     2521 gtcgatcgcg tcgccggggc ggtcggggcg tgcgaggcgc acctcgtggc cggcataggc
     2581 gctcagcgct tcgctgaagt cgccgtcgac gacgtgcgcg gccacctggc gaccgtagaa
     2641 gttgacctcg agcgcctcgc cgtcgggctc agccgtcccc gcaacgacca cgccgttcgg
     2701 gagccggagc tcgagccggt cggcggcctc gtcgtaggtg gcctcgatcg gcaggatcgg
     2761 agccttcgtc gcgccggaga accgcctgcc ggatccgtcc acgaggaaga agcggcggtc
     2821 gccggcagcg ccgcgttcgt ccagtcggat gcgctcggga tggtgcagcg cggtcgactt
     2881 gacgggcgtg acgttgaagc gcgcgacggt cggcatggcg acacgctacc aacccggtcc
     2941 gacggcgggc gcgtggtagc gtcgggcccg atggaggtcc ggcaaccgaa cgcggaggtc
     3001 tcggcgcgcc ggccgatggc gacgccgtcc caactccacg ccgacctggc cgagctggcg
     3061 ttcctgctcg gcgactggag cggcaccggg gagggggtct ggccacccgg cgagcggttc
     3121 gactacgccg aggacgtgac gttcgagttc gtcggcgatc cgttcctgtt gtatgcgcag
     3181 cgttcctggt cgctcgacga cggctccccg atccatttag aacgcggttt ccttcgcccc
     3241 gcgggcccgg gcatcgtcga actcctgctc gcccacccga tcgggatcac cgaggtcgcg
     3301 gtcgggaccg tggacgatgg cctgatcgag ctctcgtcga ccgccgtctc gctcgcgccc
     3361 acggccagcc cggtcaccga gcttcgtcgc cgcatccagg gtcgaggcga cgagctctcg
     3421 ttcgagctgc agatggcgat ggagggcgtc gagctccggt ggcacgtcgg ctccaagctc
     3481 acgcgcgcct gaggccgatg ccgtcagccg ccgccggcgc acgtcgagca cgaccggtcg
     3541 cggcgggtcg atcccgatcg gcggccgcgc ctcaagcgcc gcgcgaacgc ttgccgggca
     3601 agaacacctt gaaggaggcg cccccgccct cgcgctcctc cacccaggcc cttccgccgt
     3661 ggagctcggc gaagcgggag acgacggcga ggccgatccc gacgccgggg gagtgctcgg
     3721 gcgcgccggc gccctgaccg aacggctgga agatgccctc gcggtgttcg ggtggcaccc
     3781 cggggccgtc gtcctcgacg acaagtaatg caccgcccgc ctcggggccg acgcggatcc
     3841 agaccttcga gcccgcgggg gtgtgtcggg cggcgttcga gagcaggttc tcgaagatgc
     3901 gctcgatctt cgcggcgtcc gcctcgatcg tcaccgggtc gaggtccagc tgcgtcgggt
     3961 gttcggcgag gaggtcggat tcgatcacga cgcgccgaac gagctcgtcg agatcggtgg
     4021 cgcgcaggtt cggctcgagg atcccacgcg agatccggtc gaggtccaac aggtccgaga
     4081 cgatccggtc gagcttgcgc gcgttcatcg agatgcgcga cgcgagatcg tgcgcctcgt
     4141 cggacccgag gtccgtctgg ctctccaacg tcacggcgag gccgaggatc gcggcgagcg
     4201 gcgtccgcag gtcgtgcgag acggcctgca ggaacgtcgt cttgagctcg tcgatcgcgc
     4261 ggagctcggc ggcctcctcg cgctcggcct ggagcgcgcg ttcgagctcg gccgcgcgct
     4321 tctgctcggt caagtcgatc catgcgccct gccagaactt cggcgtgccc tggtcgtctc
     4381 ggatcagcgc cgcctggtcg tgcacccaca cgacgcggcc gtccttcgcg atcatccggt
     4441 actcgacgtc gtacgtgccg ttcgtctgct cggcgagcag ggacgccccc aggatccgtt
     4501 cgcgatcgtc cgggtggacc agctcgggcc acaaggggat gtcgcgtgtc agttcgtccg
     4561 gggtgtagcc gagcagggcc tcgacctgcg ggctgaaata cgcgctcgtg tggtcgggtc
     4621 ggtgcgggtc gatgtaggtc acgaccggcg tctgttcgac gagcgctcgg tagcgctgct
     4681 ccgcctcctg gagccgatcg agcgcggccg ggcgttccac cgtctcgtgc atcacgccct
     4741 gccagtagag cggggcgccg tcctcgtcgc ggaccaacac ggcctcgtcg tggatccaca
     4801 cgatccggcc gtccttggcg aacatccggt agtcggcgct gtacggcgcg ccggtcggga
     4861 cgacggcctg gtacgcggcc cgcatcgccg gtacgtcgtc cgggtgcagg cgttccaccc
     4921 acccggcggg atcggccaac cactcctcga cggtgtagcc gaggatccgt tccacgcccg
     4981 ggctgacgta caggaacggc cccggaccct cgatccgcgg gtccgcgtcc tcgatataga
     5041 ccatgccggg gaggtcgcgg agcagcgccc ggaggcggtc gagctcggcc tcgggctcgt
     5101 cgcccggggg caggcgttcc ggctcggtgg gcgtcatcgc gaagggatcg tagcccggtg
     5161 gcgggatgat ctcagcgcgc cctcggcaaa ggtgcctacg agcctcggag gacgagcccc
     5221 cggtagccgt cgacgaaccc ggccgtgcgt aggtcctcgg cgaacgccga tccgccaact
     5281 ggctcgccgt cgatcttcga gagctcgatc cgccgcagcc ggccgtcctt gaccagcgag
     5341 gcgagggcgt cgatccacgc cccgtccccg cccgccgcga acgtgagcag ggtccgagcg
     5401 ccgcgctcga ggaacaccgc gggctcgccg tccgcgagca cgacgtaggc gccagcggtc
     5461 cgcgaggggc gcccgcttcc ctgcccggtt ccttggggcc agggcagggc cgccccgtac
     5521 ggctgggccg ggtccgccgc ggcaagcgcg aggaccgacg cctcccgcga ggtctcgcgg
     5581 aggtcgcgga tccgttcgac cgcccccgga agcgcgaact gcgccgcgcc gagaccgtcg
     5641 acgaagtagc cccgccggac cttgcccgac tcctcgagcg cccgcagcac cgcgtacacg
     5701 ccggcgaacc cacccggcgc gccttcggcc aggaccgcct cgcgcgtgag cacgccgtgg
     5761 cgttcgagga gctgcatcgc tcgcgcgtgc gcagcctcgg tgggcgaggg ctccggttcg
     5821 agcagcgttc gcacgagcga ccaccggccg gcggccgagg gcggcccggc gcgccggagg
     5881 gctcccggcc ggggcttggc gcccgagcgg gcggccttgg tcgggcggcg gatcaggaat
     5941 gcgcgcatcg gcgcgatcgt gtcgttggtg acctcgcccg cccagaccag gtcccacagg
     6001 gcggcgagca cgatgcgttc gtcggggatg cctgccgcgg cgagcagctc gggccagaag
     6061 gacgcgcctc gttggcgcag gtggtcgcgg atcgcgtcgt ggaccgggcc cgccggcggg
     6121 gtctcgggaa gcggcggcgc agcgagccgg agcgtctcgc ggaacccgag ggcgatcctt
     6181 ccgtcgtcgg cgccgatccc gccggcgccc atccacacga cgtcgccgct cgcgcacagc
     6241 gcgtcgagat cggccgggcg gtagccccgc acacgcgacc gcaggacctc ggactcgacg
     6301 atcgaggccg ggatcgaggc gccctgcagc tgcgcgatcg catcgacgag cgcggtcggg
     6361 ccgtgccgcg ggctcgtcgc ggcctgccac tcgggcacga acctcccgag cgcgggtgcc
     6421 tcgaccggtt cgacctcctt gcggagcgcg gcgagcgagc gccgccggat cgaccgcagc
     6481 acgtcggcgt cgcaccactc gcgctcgaga ccgcccggcc ggaactcgcc ccggacgacc
     6541 cgcccctgcg cctcgagcga tgcgacggcg ccggcgaccc ggtcctcgcc gatgccgaat
     6601 cgctccgcca cctcggcggt cccgaacggc ccatgggtcc gggcgaaccg cgcgacgagc
     6661 ccctcgagcg gcgcgggcgt cgcctccgtg aagacgccgg ggacgccgag cgggagggag
     6721 gcgccgagcg cgtcccggta gcggcctgcg tcctcgacgg cgacgaaccg gtcctgcccc
     6781 gcgacccgca tgcggatcgc gcgcccgtcg cgttcgagat cgtcgagcca cgcggacgga
     6841 tcggcgttcg agcgcgccga gacctcctcg gtgctcaggt gtccgagccg ccggagcagg
     6901 tcgtgcacgt cgtcggcgtt ccgagcgcga cggcccgcgg ccagatgctg cagctcgagc
     6961 tcgagctcgg cgagcgcgac cgggtcgatc agctcgcgca ggtcctcggc gccgaggagc
     7021 tcgcgcagga gctcccgatc gagcgagagc gcggcggccc gacgctcggc catcggtgcg
     7081 tccgcctcgt acatgtagac cgagacccag cggaacagca gggactgcgc gaacggcgac
     7141 gaccgcgggg tgtcgaccgg caccacgtgg atgcgccgcg cccgtacgtc gccgagcacc
     7201 tccctcagcc cgggcacgtc gaacacgtcg cgcaggacct cgcgcgtggc ctcgagcagg
     7261 atcgggaacg tggggtgatg cgaggcgacc tcgagcaggt ccgcgccgcg ctgccgctgc
     7321 tgccacagcg gtgtccgtcg ccccggcgta cggcggggga gcaggagcgc gcgggcggcc
     7381 gcctcccgga acatcgtcgt gaacagcgcg gtcccgggga gccgggcgac cacgagctcc
     7441 tcgatctcgt cgggatcgaa caagagatcc tcgaccggga tccgctcctc ggactcgggc
     7501 aggcggatcg cgatgccgtc gtcgctccac agcacctgga cgtcgagtcc gtagcgttcg
     7561 gccagccggc cctcgatcgc catcgcccac ggtgcgtgca ctcgctcgcc gaacggcgaa
     7621 agcacgcaca cacgccagtc gccgatctcg tcggggaacc gttcgaccac gatcgttcgg
     7681 tcgtcgggga ccgcgccggt cgcctcgcgc tggtcgtcca ggtacgccgt gaggttctcg
     7741 gcggcgagct cgtccagccc gaactcggtc cgcagacgcc cgacggccgc gtcgtgctcc
     7801 gacgacgaga gctcgcgcgt agcggcgccg atcgcgcgtc cgagctcgag cggccgcccc
     7861 ggcttgtcgc cgtgccagaa cggcatcttg cccggctcgc ccggcgccgg cgagaccacg
     7921 acgcggtcga gcgtgatgtc ctcgatccgc cacgacgacg cgccgagcat gaacacctcg
     7981 ccgcgccggc tctcgtacac catctcctcg tcgagctcgc ccacgcggcg accgtcgacc
     8041 gtgaacacgc cgaagagccc ccggtcgggg atcgtcccgc cggaggcgat cgccagcctg
     8101 ccggcgccct cgcgtccccg aagcgtgtcc gtccgccgat cccagacgat ccgcggccgg
     8161 agctcggcga actcgtcgga gggatagcga cccgccagca gatcgaggac gttgcggagc
     8221 aagtcgtccg agagctcctc gaagttcgcc gcacgccgca gcacgcgacc gagatccccg
     8281 acgtcccact cgtccatcgc gcacatcgcg acgatctgct gtgcgaccac gtcgagcggg
     8341 ttccgagggt aatgcgtctc ctcgatcgcc ccccggagca tccgctgcac gacgaccgtc
     8401 gcctcgagca ggtcgccccg gaacttgggg aagaccttgc cgcggctcgg ttcgcccacc
     8461 tgatggccgc cgcggccgat ccgctggagc ccgcgtgcca ccgagcccgg cgactccacc
     8521 tggatcacga ggtcgaccgc gcccatgtcg atcccgagct cgagcgagct cgtcgcgacg
     8581 atcgccttca gggtcccccg cttgagctgg tcctcgatct ggagccgctg ctcgcgggcg
     8641 atcgagccgt ggtgggcccg ggcgagctcc tcgccggcga gctcgttcag gcgggcggcc
     8701 agccgttccg ccagccgtcg ggcgttcacg aacacgatcg tcgaccggtg gccccggatc
     8761 agctcgagta cctggggatg gatcgccggc cagatcgacg cccggggcgg cgcggtcccc
     8821 ggcgtcgcgg gcccgagcat cggtggcatc cgctgcgggg tctcggcgac gtccgacagg
     8881 tccgccatgt cctcgaccgg cacgacgacc tcgacctcca tcggcttgcg gagcccggcg
     8941 tccacgattg tcacgggacg cgggccgctc ggggtctgtc cgcccaggaa ccgcgcgatc
     9001 tcctcgagcg gacgctgggt cgcggacagg ccgacccgct gtggcggctc gtgtgcgatc
     9061 tcttcgaggc gttcgagcga cagcgcgagg tgcgcgccgc gcttggagcc ggccatcgcg
     9121 tggatctcgt cgacgatgac ccagcggacg ctccgcagca cgtcccgcgc ctgtgaggtg
     9181 agcatcaagt agagcgactc gggcgtcgtg atcaggacgt cgggcgggcg cgtctggatg
     9241 cgacggcgtt cggcggcggt ggtgtcgccg gtgcgcatcg cgacgcgcgg ctcgtgcacg
     9301 tgctcaccga gtcgctccgc cgccagcccg atgccgacca gcggcgtccg caggttcttc
     9361 tcgatgtcga ccgccagcgc tcgcagcggc gagatgtaga ggacgcggca tcggcgatcg
     9421 gtgggcgggg gcggatccgt catcacgcgg tcgagcgccc ataggaacgc cgccagcgtc
     9481 ttgcccgagc ccgtgggcgc caggatcagg ctgtgccggc cgccggcgat cgcgggccac
     9541 ccctgcgcct gcgccgcagt gggcgtctcg aacgtgctct cgaaccacgc tcggacggcg
     9601 ggggagaacc cctccaggct catcggatca ccgtaccgcg aacgcacgac acgaccgctc
     9661 ggacctcaag cttccgggtc gggctcgagc gtgagctggt agatctcttc gccctccagc
     9721 cagtcaccgg tcggctcgaa ccccatcgct cggtagaacg gagccggcga acccgcaccc
     9781 gggtgaacgg acgtgacgag ctcggtcgcg ccgggccggc cgcggagctg atcgatcacg
     9841 acggtcaccg ccgcgcgacc gtagccatga ccttggtgac cggcgtcgat cagaagccgc
     9901 caaaggaagt agcggaacgg ttgctcggga tcccccggcg gcatgtcgta ggacagcatc
     9961 acgaatccga ccggccgctc tccggcgtag atcgcccgga accaaggccg cgcctgaggg
    10021 tcttccagcg agtctgccac cgacgagacg aaccgctcct ggtccggggc gacgcggagc
    10081 gcgcgaacgt cagcctcgtt cgccgaggtg atctcacgca acgtcacgac ctcgtcggac
    10141 acgccccaga gcttacgaac gctccttctt gccgcgcgga taggatgctc ggcgatgcaa
    10201 cgcactcgca ccgggatcgc cctgatgctc ggaggcttct gcctggtagt ccctgctctg
    10261 gcgtccgccc agaccgatcc ggccgatgcg gagccgcggg accagatcgt cctgtccggg
    10321 gacatcctgg tccggcgcgg ccaggaggtc ggcgaggtcg tcgtcctcca cgggacggcg
    10381 acgatcgcgg gggtcgtccg cggcgacgtg gttgtgctcg acggccggat cgaggtcacc
    10441 ggccaggtca gcggcgcggt cgtgagcgtg agcggttccg tcgggctcgg accgagctcg
    10501 cgcgtgctgg gcgacgtgct cgcgcacgag cgcgtggtcg cggcacccgg ggcccgggtg
    10561 gacggggacg ttcgtgaggg cgtcacgttc acgctccgag cgccgatcga ggcgctcgga
    10621 gccttcgcgc cgtggctggc ggtctggtgc tcgctgctcg ccctcggggt gctgctcctg
    10681 ctcctcggcc cgagggccgc cgacgcggtg tcgtccgcgg cgcacgggtc gctgtgggcc
    10741 tcggtcgggt ggggattcgc ggcgttcgtc gcgctcccgc tcgcgggcgt gatcggcgtc
    10801 ttctcgctgg tgtggctccc cctcgggctc ggtctcctgc tttcgctgtt cctcctgtat
    10861 tcggtgggtg tcgcttggag cgcgttcgcg ctcggccggg tgctatggca cgagccacgg
    10921 agccgcacgc tcgcgttcgt catcgggtgg gcgatcctgg ccgcggtcgc ggcgatcccg
    10981 ttcgtggggg gcgtcgtctg ggtggccggg gcggtgttcg gagtcggttc gagcaccatg
    11041 gcgatctggc gcgcccgccg ggcggctccc cgagcgggcg gccggcaccg tcagggtggc
    11101 aagatgccgg tggtgatccc ggcctcgtcg gacgagccgg agccgatgat cgtcgaacgc
    11161 gagatgggcg aggagggcgc gggtttgtga gcgacacggc cgggctggtc cggaaggtcc
    11221 cgatgttcgc cgagctcgac gacgacgaga cgacgtgggt ggccgatctc gccaccgagt
    11281 tcgaggtgcc cgccggcttc gtgttgatcg agcgcggtca gcccggttcg ggcatgttca
    11341 tcatcaccga cggaaccgtg acggtggaac tcccgcaggg gccggtcgag ctcggccggg
    11401 gggagttcgt cggcgagctg ccgttgttgg ccgacgggat cacgcgcgtg gcgcgggtcc
    11461 gcgcgacgac cccggcgaag ggtctggcga tctcacgcat ggccctgtcg gagctcctcc
    11521 agcgggagcc gcgcgtggcc gtccggatgc tgcccgtcct cgctcggcgg ctcgccgagg
    11581 ccgagtcccc ctagcgcgaa cgccggcgac gccggcccgc gatcatcgcc acgagcgccc
    11641 cgaccgccgc accggcggcg aacgcgcccg gcgtcccgag caccacgcga gcgccctcct
    11701 cgatggtgac cttcggcgag gcgacgaggc ccacgaacgc gccgtggccg accgtcgcgt
    11761 tgccggctgc gagcacgctc tgcgtcccgc cctgctggat cgacaggttg ccgcgaacca
    11821 tcgccggccc gcagccacct tggtggatcc ggaggtcgcc gccgacgagc acgggtccgc
    11881 accccccctg ctggatccgc acgtcgccct gcgccgcgat cggacccgct cccgcctgtg
    11941 tgatcgacac gtcccgcccg ttgacgacgc cgacgagcgc gcgttcgacc gtcacgtcgc
    12001 ccgccgcctc gaccgtcttc aagatcccct gctccgcgtg ctcctcgacc ggctgatcca
    12061 tctgcgacac ctcctcactc catcgtaggt accctcggcg cgtggcgcag aaggctcgaa
    12121 ccttcctgtt gatgggctcc ggcgagttcg agccgtggtc gaacgagatc gaggccgcgg
    12181 cgctcgacgg tcggaccggc cccgtcgccg tgctcccgac ggcgagctcg accgagggcg
    12241 aggacgtatt cgggcggtgg gggcggatgg cgctggagca ctacgcgacc gcggggatcg
    12301 atgcacggct cgtgcccgtc aagacgcggg aggacgccga tcgcgaggag cacgctcggg
    12361 cgctcgaggg ctccgcgatg gtcttcttct ccggcggcaa gccgcagcac ctggcgacga
    12421 cgatccacgg gacccggctg tggcgggcgc tcgaggacgc cctggacgag ggtgcggcgt
    12481 tcgccgggtg cagcgcgggc gcgctgatcg cgagccagtc gcgcgagcag cggcgcgaac
    12541 gcggcgttcg gaccgggtgg gtcttcgggc tcgggctggt gccgcacgtc tcgttcggcg
    12601 tgcactggga caaggtgaag gtgatcccgg gccttcggtc ggtcgtgatg tcgcggaccc
    12661 cgccgggttc gtggttcgtc gggatcgacg agaggacggc catcctgggc gacgggcagc
    12721 gctggcgcgt gtacgggctc ggcggcgtga tggtccgcca cgccggcggg acccagcttc
    12781 acctggaggg cgcgaacttc gagacgtccg atcaggggga taggggtcct gccgatatcc
    12841 ttgtcgaccg tggtctcgac cgacgaaccg atcgcgccgg agcaccagcc gacgtgctac
    12901 ctctgtggcc ggcccaccta cgaccccgag aagcgggagc ggccgtgggt gcgggcgacc
    12961 tcgggcggac gccaggtcct ggtctgcccc tcatgccagg aggagcgacc ggactgggcc
    13021 gttcagctcg accggtgcga gtcctgcggc gagactcggc tcacggcgat gctcggacag
    13081 gtcgtgtgca gggcctgcga tcacgtcagg ggcgaaccgg tcgagcccgc gtggttgacg
    13141 acgggcgctt agctcagagg tagagcactc gccttacaag cgagcggtcg gaggttcgag
    13201 tcctccagcg cccaccactg aaaccgcagg tcagcgcttc gcagcgtcga tcgggatcac
    13261 gtgttcgtgg tcggcgtgcc gcagacgccc gaggccggcg gcacgaacga tctctccaac
    13321 ctgcgcgccg tgtgccggcg ttgccatgcc cgccgtaccg gccagcaagg ggcgctggcc
    13381 aagcagcggg ctatcgccaa gcgccgcgct cagcagcgga aacctctcac gagaggagga
    13441 agcggatgaa tgaacaggtc gaacggtcgc gaaaggaagg gggactgaac atgttttggc
    13501 cgcaggaaga agggacccga caaggcgttc acccacgccg aggactgcaa gatcctggcc
    13561 gccgatccca gcgtgcggat cccgtggagc gagatccgcg gtggagtctg ggaagccgtc
    13621 tgcgtctgcg gggtccagta tcagcatgac cctatcgtcg acgatcgtgt ccggaacgat
    13681 ccgctgaaga tcggccacgc ggtgcgcctc agtgcgagct caacggcgag accgatcccg
    13741 ccgtgctccg gttcgctctg aaggccgtcg aaagggacgg ctattggtgg gtgcagtgca
    13801 gtgcctgcga ctccggatgg caggttccgc actatgccga gagcgttagg tgacgacgcg
    13861 tcccttccca gggttggttt cgagtcacct gtccagcgcg aacccatgcc gcttggagag
    13921 gtcctcaagc agttggaaca gttcatcctc cggtatcgac gagagcctga gggcaagcgc
    13981 ggcatgtgcc agcggctcgg gatcaccagg acgccgcccg gccatgaggt cgtaggcacg
    14041 cgtgaaccag ttcctgaccg tcgcctcttc gtagtcgatg tacaagtatt cgcccaccaa
    14101 gtattcgccc aacagggggg cctgcacggg cggggccgca atccggacct ttcggacgcg
    14161 atcccatggg atacgctccg tcttccagga gaaagagtcg tccagcgggt ggtaggtcac
    14221 gccggtcggg tcgatctcga acacgggctt gacagctctg cgcaggaagt gaacgccccc
    14281 ctcaccgaac gccccgccaa ggacgacgga gaacgcagcg acgggccagt cgcgccagtt
    14341 tgtgagcacg agtccgccgg cggcgatgac cagcatggtc cccagcgcca gcatgagcac
    14401 gccccagacc ctcgtaccca gggacttcga cgagaccgtg acgcgccagt cctgggacgc
    14461 ccttgccctc atctattggt tatcggtcgg acgagcacaa cgactaagcc cggcctggag
    14521 cgagactcct tggcagggta attcgcgtgc gacacggcct ggcaggttcc gtacttcgcc
    14581 gagagcgtta ggtgacgaga cgtgggaggg gatcccgatg aggtccgacg gcgactggcg
    14641 tcaggcgatc cggaagctct cctccacaat cagctgcgac ccgacgtgct cctcgaaggc
    14701 gctcggcgag gccgtgaacg tgatcacata cccggtgtcg ccccggacct ggtacgcctg
    14761 tcgttgacgg agcgccgtcc cttggctgtg ccagcgatag tccatccacc gagcgggctg
    14821 cccgtcgatc cgcgtccgcc cctggtcgac gagctcgaaa ccggcgaaca tgccagcgac
    14881 ggtctgcttc gacgccagga agtactgatc cgtgctcagc tcgagtcgga gaggctccaa
    14941 gacgacgttg atgttgggga ggatcccgtc ctgccgagcg gcgggcggcg cggagatgat
    15001 cacccccacg aaccccgtcg cgggttggct aggcgtccag gacgccggat acgagaggga
    15061 gtatccgtct ccctcgtagg tctgggtatc cactgaagaa cccgagggac cggtaggggt
    15121 ctcggaggaa ggtgtctcgg aggaagcggg attgcccgcc gaagcgcagg ccgtagccca
    15181 caggactgag gaggctagga ggggcaagca gagcctcgtg gaccaaggac gcccggatga
    15241 tcggttggtc tccatcgtag ttccatcggc gcatctgagt ggagggttga accccactca
    15301 ggcgcggctc gagttcctcg gccgcaccgc cggctcgccg tgcggcgcgg gctccgggtg
    15361 cgcccaaagc cccggtgaag ggccccgcag catgagcgtg gtgaggtcgg gggttctggt
    15421 cgatgtcgct tggccctcat ccccccatca agcgaaaaac tactcgagct cagagcgggc
    15481 cgccgccgtg ccacgccgcc ggccggcagg tcgagacata gtgctggctc gaaaggtcca
    15541 atgggatccc ttggaatccg tgcccctgtc gaccgggcgg tgcctcgaag ccgctgctgc
    15601 ggcgatggcc cggcgggatc ggcgggacga ggtgcgtgga caggagccgg tcggcctcgt
    15661 cgaagacctc gacgaagcac aagacgatcg tcgacgactc tccgggattg accgacgtca
    15721 cgaggcaaca gcgcccgtcg gatcgtggct cgagaagcag ggtcacgcct tcgctcgtgg
    15781 agctctgcgt cggtcccgtc tccgaaccac caggaacggc gggagtcgac cgatcgaatg
    15841 ccccgccgct cgtcacgatc cagagcaccg caacaaggat cgtgatcccg acgacacccg
    15901 ccgcgatgcg ttcgttgcgg cgattgcggt cgcgtcggcg cagcgtccac tcaaccatct
    15961 tcagcgtctt catctcacct gcctggttag cgctcggatt ctcgcagcat gccgacgagc
    16021 accgctcgga ggacccggtc ctccttgcag tcgatcagca gctcggccag ggtagagcga
    16081 tgggcatcta gggcggcatc gccttcaagt tcagactctt ccttgagtga agctgccggc
    16141 tgacgatgcc gagaatgtcg ttggtggagt ctcggctgag gacaccaata tgaagcggtc
    16201 gtcttggcat cttctcgtcg tgggagctgt tctcaattgt ctaggtttcg tcctgagcgc
    16261 tctctggacg gcggagatgg ctacgtcatc caaagcatcg gaatcgttgt ctggctgaac
    16321 atactgctcg tactgggagc ctcttatgga cggcgttgga ccgtctacgt tctcgcagga
    16381 gtatccctcg tgagcggcgt agcgcagggt gccacattag agccaaggcg gttcatcatt
    16441 agcgcgatcg agttgggcgc agcggccgcg tgtattgctt acctcgccca gacacgaagc
    16501 gaccaaacgc aggcccggag ctcgttctcc gcttctcgca gcatcccgac aagcaccgcc
    16561 cgcagcgtcc cggtcctcct cgcagtcgat cagcagctcg gcgaaggtgc gcgtcttcgg
    16621 gttccccaga actcgccgat ccggtcgggt cgctcgaagt cggggatcat caggacgtgg
    16681 agcagttcgg tgcggggagc cgggcccgag ggccggcgca tgcaggctag ccagcagcag
    16741 tccaggtgca cgagtagtcc tccggacagt agagctgccg gatctcaagc tccacgcggc
    16801 cacccataac ccacacccct gtccgggcat cgatctcgag tccactcgtc gaatcggggt
    16861 ctttgaccac aaggttcgcc tcatccgtct cgacaatggt ccagtcgttg agcgcatggc
    16921 ggtagtagcg catcacggct cgctccccgc gatctgcctc agcatcgctc atgctggtgg
    16981 tcgacacgta ggcgtgtacc tgctcgtccg agatccccgc aacaagagcg aagtcgtagc
    17041 cgggcgggag gggcaccggc tcgcgaagcg gccccgtgct cggctc
//
LOCUS       DATBRK010000462         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1718458, whole genome shotgun sequence.
ACCESSION   DATBRK010000462 DATBRK010000000
VERSION     DATBRK010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718458"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /gene="ftsZ"
                     /locus_tag="VIH70_13475"
     CDS             <1..1078
                     /gene="ftsZ"
                     /locus_tag="VIH70_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY8117418.1"
                     /translation="AVNTDAQTLLMSDAEVKLDIGRETTRGLGAGSDPEVGRRAVEEH
                     RDEIEEILKGADMVFVTAGEGGGTGTGGAPIVAEIARALDALTIGVVTRPFGFEGRLR
                     ATQADLGISELKKAVDTLIVVPNDRLLQVSDPSTPMIDAFRTADQVLFQGVDGITSLI
                     TTPGLINLDFADVRSVMTGAGSALMGIGQGSGEDRAEEAARSAISSPLLESSIEGARG
                     LLLSVAGPTGLTLHEVNKAADAITRVAHPDANIIFGAVIDDALGEEIRVTVIAAGFDK
                     ALTAPATSRFAESRLSRLLEEPETLTVIEPVPAASSPIDDEDDVMPMPSILVDEEDEI
                     FKPAQDGVGFDAEEDLDIPDFLKS"
     gene            1283..1993
                     /locus_tag="VIH70_13480"
     CDS             1283..1993
                     /locus_tag="VIH70_13480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY8117419.1"
                     /translation="MPADPTPDALLANLTTIRERIAGACERGGRDPGEVRIVAAAKTI
                     EPEAIERVRDAGVREVGENYVKELRTKRDRVDGIVWHFIGTLQSHSAHHVAELADIVE
                     TVTPGRATERLARRAVDIGRSIPVLIEVDFTGERTGVAPEGVEVAADRVAGTDGVELV
                     GLMTLPPIPSVAEDSRPFFRRLRELRDGVSAHHPEAAELSMGMSLDYPVAVEEGATMV
                     RIGTALFGARRPEDTGRT"
     gene            1993..2472
                     /gene="sepF"
                     /locus_tag="VIH70_13485"
     CDS             1993..2472
                     /gene="sepF"
                     /locus_tag="VIH70_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEY8117420.1"
                     /translation="MPGVWKKTLTYLGLVEDDEFEELDEVGAAPETAEVRRFQRPQAV
                     REVELEPQHEGIVRTIPSSRPATAGSIHKSEPRRFNEARDIADRFKDGIPVIMNLQST
                     DDTIARRLVDFASGLVYGLDGKIEMVANRVYLLTPANVDVSAEDRERIAGGDLYNQF"
BASE COUNT          399 a          846 c          913 g          381 t
ORIGIN      
        1 cgcggtgaac acggacgcgc agacgctgtt gatgtcggat gcggaggtca agctcgacat
       61 cggccgggag acgacgcgcg ggctgggtgc gggttcggac cccgaggtcg gtcgccgggc
      121 cgtcgaggag caccgtgacg agatcgagga gatcctcaag ggcgccgaca tggtgttcgt
      181 cacggcgggc gagggcgggg gcaccgggac cggtggtgca cccatcgtcg ccgagatcgc
      241 ccgcgcgctc gacgcgctca cgatcggagt cgtgacgcgg ccgttcgggt tcgagggtcg
      301 tctgcgcgcg acgcaggcgg acctggggat cagcgagctg aagaaggccg tcgacacgtt
      361 gatcgtcgtg ccgaacgatc gcctactcca ggtctccgac cccagcaccc cgatgatcga
      421 cgcgttccgc acggcggacc aggtcctctt ccagggcgtg gacggcatca cgtcgctcat
      481 cacgaccccg ggcctgatca acctcgactt cgccgacgtc aggtcggtca tgacgggggc
      541 ggggtccgcg ttgatgggga tcggtcaggg ctcgggcgag gaccgcgccg aggaggccgc
      601 tcgatcggcg atctcctcgc cgctgctgga atcctcgatc gagggggcac gtgggctgct
      661 gctgtcggtc gccgggccca ccggcctcac cctgcacgag gtgaacaagg ccgcggacgc
      721 gatcacccga gtggcgcatc ccgatgcgaa catcatcttc ggcgccgtga tcgacgacgc
      781 cctgggcgag gagatccgcg tgacggtgat cgccgccggg ttcgacaagg cgttgaccgc
      841 gccggcgacg tccaggttcg cggagagccg tctgtcgcgc ctgctcgagg agccggagac
      901 cctgacggtg atcgagccgg tcccggcggc atcctcgccg atcgacgacg aggacgacgt
      961 catgccgatg ccgtcgatcc tggtcgatga ggaggacgag atcttcaagc ccgcgcagga
     1021 cggggtcggg ttcgacgccg aggaggatct cgacatcccg gacttcttga agagctagtt
     1081 ggccgaacgt tcgccccgca ccgtgggggg cgggatcgtg ttcgagcggt cagaggcgac
     1141 cttcccccgc ctctggggcc cggggtcccg cccccccttc catgaccgga tcacacggct
     1201 ctcccgaaca ccgccgggtc ggtgctgatg ttgttcgtgt gcctcctgtt cctgactgtg
     1261 agcttctgtt aggttgcgtg acgtgccggc cgaccccacg cccgacgcac tcctcgcgaa
     1321 cctgacgacg atccgcgaac ggatcgcggg cgcatgcgaa cgcggcgggc gagaccccgg
     1381 cgaggtccgc atcgtggccg cggccaagac catcgagccc gaggcgatcg aacgcgtccg
     1441 cgacgcgggc gtccgcgagg tgggggagaa ctacgtcaag gagctccgca ccaaacggga
     1501 tcgggtggac gggatcgtct ggcatttcat cgggaccctg cagagccact cggcgcacca
     1561 cgttgccgag ctcgccgaca tcgtcgagac cgtcacgccc ggacgcgcga cggagcgtct
     1621 cgcgcggcgc gcggtcgaca tcggccggtc gatcccggtg ttgatcgagg tggacttcac
     1681 gggcgagcga acgggcgtcg cgcccgaggg ggtggaggtg gcggccgatc gggtcgccgg
     1741 gaccgacggc gtggaactcg tcggcctgat gaccctgccg ccgataccgt ccgtcgccga
     1801 ggactcccgc ccgttcttcc gccgtctgcg ggagcttcgg gacggggtgt cggcgcacca
     1861 tcccgaggcg gcggagctct ccatgggaat gtcgctcgat tacccggttg cggtggagga
     1921 aggcgctacg atggtccgga tcggcacggc cttgttcggg gcacggcgcc cggaggatac
     1981 agggaggacg tgatgccagg cgtgtggaag aagacgctca cctatctcgg cctcgtcgag
     2041 gacgacgagt tcgaggagct cgacgaggtc ggcgccgccc ccgagaccgc cgaggtccgt
     2101 cgcttccagc gaccgcaggc cgtccgcgag gtggagctcg agccccagca cgaggggatc
     2161 gtgcgaacga tcccgtcgag tcgccccgcg accgcggggt cgatccacaa gtcggagccg
     2221 cgtcgcttca acgaggcgcg cgacatcgcc gatcgattca aggacgggat cccggtcatc
     2281 atgaacctgc agagcaccga cgacacgatt gcccgcaggc tcgtcgactt cgcgtccggg
     2341 ctcgtgtacg ggctcgacgg caagatcgag atggtggcca accgcgtcta cctgttgacg
     2401 cccgcgaacg tggacgtctc cgccgaggac cgcgagcgca tcgccggcgg cgacctctac
     2461 aaccagttct gacgccgctc gatcgttcga cccgtccgcg atgaccgccg ccgagaacgc
     2521 gctgctcagc ctcctgtgc
//
LOCUS       DATBRK010000463         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_139165, whole genome shotgun sequence.
ACCESSION   DATBRK010000463 DATBRK010000000
VERSION     DATBRK010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139165"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VIH70_13490"
     CDS             <1..1087
                     /locus_tag="VIH70_13490"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY8117421.1"
                     /translation="LEEKVAMLMEATRRMQSVEGLSFAEGGIDLFRRRTFLATSEGTA
                     IEQTTVQSGAGIEGTALGDGELQRRSFPNSFRGHISAAGWEHIQRLGLIEEAERTATE
                     AVELLSAEECPSEVTTLVLDSGQVELQIHESIGHPVELDRVLGMEEAYAGSSFVRPED
                     RGSLRYASPLVSITADASLPGGLGSFGWDDEGVPAQRVPILVDGVFENFISSRETASV
                     LGLTSSGAMRADGWANYPLIRMTNISIEPREGSLADIIGDTDDGVFMSTNTSWSIDDH
                     RVNFQFGCEIGWRIEKGRLTTMYRNPNYTGITTEFWGSCDAVGGREEWTLWGTPNCGK
                     GQPGQVARVGHGASPSRFRGVQLGVRG"
     gene            1102..2454
                     /locus_tag="VIH70_13495"
     CDS             1102..2454
                     /locus_tag="VIH70_13495"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY8117422.1"
                     /translation="MKTGLIGPDEVRRIADGALNLPRADGVEVLFMHEWGGLTRFASS
                     SIHQSTYREDTGIRVRVVSEGRIGVTATNDFSPAGALRAAESALEMASVVAPDPLFPG
                     LAPAAPTPEPQAFDEPTAETRPSERAEAVAELVERCPEGFTAAGAYETLASEVAVANT
                     EGQFCWAPSSQASVTTVVSGPDGGSGFAEMFAARRSELDPAAVGKRAADKAVASRSPR
                     DLEPGRYTVILEPAAVSTLVGFLAWIGFGGRSLFEGRSCFSGKQGQQVAAPIVRIYDD
                     ALSDGTLGLPFDFEGVPRSRVDLIEDGVFRSGVYDLRTAKQAGTTTTGHALPPPNPEG
                     PFPLNLFMAPGEAEVDEMIASTEHGLLVTRFHYTNIVHPVESTITGMTRDGTFLIRDG
                     RIAHPVKNLRFTQSILEALTGTEMIGRETELASEFFFSASRVPALKVSSFNFSGRSDH
                     "
BASE COUNT          391 a          915 c          977 g          390 t
ORIGIN      
        1 gctcgaggag aaggtcgcga tgctgatgga ggcgacccgc cggatgcaat cggtcgaggg
       61 gctgtcgttc gccgagggcg ggatcgacct gttccgccgt cggacgttcc tcgccacgag
      121 cgagggcacg gcgatcgagc aaacgaccgt tcagtcgggg gcggggatcg agggcacggc
      181 gctgggcgac ggcgagctcc agcgccgctc gttcccgaac tcgttccgcg gccacatctc
      241 ggccgccggc tgggaacaca tccagaggct cggcctgatc gaggaggccg agcggaccgc
      301 gaccgaggcc gtcgagctgc tctcggccga ggagtgcccg agcgaggtca ccacgctcgt
      361 gctggactcg gggcaggtgg agctccagat ccacgagtcg atcgggcatc ccgtcgagct
      421 cgaccgggtg ctcgggatgg aggaggcgta cgcggggtcc tcgttcgtcc gacccgagga
      481 ccgcggttcc ctgcggtacg cgagcccgct ggtctcgatc acggccgacg cgtcgctgcc
      541 gggcgggctg ggatcgttcg gctgggacga cgagggcgtc ccggcgcagc gggtgccgat
      601 cctggtcgac ggggtgttcg agaacttcat ctcgagccgg gagacggcaa gcgtcctcgg
      661 gctcacgtcg tccggagcga tgcgggcgga cggctgggcg aactatccgt tgatccgcat
      721 gacgaacatc tcgatcgagc cgcgcgaggg ctcgctcgcc gacatcatcg gcgacaccga
      781 cgacggggtg ttcatgtcga cgaacacgtc ctggtcgatc gacgatcatc gcgtgaactt
      841 ccagttcggg tgcgagatcg gctggcggat cgagaagggc cggctcacga cgatgtaccg
      901 gaaccccaac tacacgggca tcaccacgga gttctggggc tcgtgcgacg ccgtcggcgg
      961 tcgcgaggag tggacgctgt gggggacgcc gaactgcggg aaggggcagc ccggccaggt
     1021 cgcgcgggtc gggcacggcg cctctccctc gaggttccgc ggcgtgcagc tcggggtgcg
     1081 tggctgatgc gggcgggcgc gatgaagacg ggtctcatcg ggccggacga ggtccgccgc
     1141 atcgccgacg gggccctgaa cctgccgcgc gcggacggcg tggaggtcct gttcatgcac
     1201 gagtggggcg ggctcacgcg gttcgcctcg agctcgatcc atcagagcac ctatcgcgag
     1261 gacacgggga tccgcgtccg cgtcgtatcc gaggggcgca tcggcgtcac cgccacgaac
     1321 gacttctcgc ccgcgggcgc gctacgggcc gccgagagcg cgctcgagat ggcgagcgtg
     1381 gtcgcgcccg accctctgtt cccgggcctc gcgccggccg cgccgacccc cgagccccag
     1441 gcgttcgacg agcccacggc cgagaccagg ccctccgaac gcgccgaggc cgtcgccgag
     1501 ctcgtcgagc gatgccccga ggggttcacg gccgccggcg cgtacgagac gctcgccagc
     1561 gaggtcgccg tcgcgaacac cgagggccag ttctgctggg cgccgtcgtc tcaggcctcc
     1621 gtgacgacgg tcgtgtccgg gccggacggc ggcagcggct tcgcggagat gttcgcggcc
     1681 cgacggagcg agctcgaccc ggcggcggtg ggcaagcggg ccgcggacaa ggcggtcgcc
     1741 tcgcggtcgc ctcgcgacct cgaacccggc cggtacacgg tgatcctgga gcccgcggcc
     1801 gtctcgacgc tcgttgggtt cctggcgtgg atcggcttcg gcggtcggtc gctgttcgag
     1861 gggcgctcgt gcttctccgg caagcagggg cagcaggtgg cggcgccgat cgtcaggatc
     1921 tacgacgacg ccctgtccga tgggaccctc ggcctgccgt tcgatttcga gggcgtgcca
     1981 cggtcgcgcg tcgacctgat cgaggatggg gtgttccggt ccggcgtcta cgacctccgc
     2041 acggccaagc aggcgggcac cacgacgacc ggccacgccc tgcccccgcc gaaccccgag
     2101 ggtccgttcc ccctgaacct gttcatggcg ccgggcgagg ccgaggtcga cgagatgatc
     2161 gcgtcgaccg aacacgggct gctcgtgacg cgcttccatt acacgaacat cgtccacccc
     2221 gtcgagtcga cgatcaccgg gatgacgcgc gacgggacgt tcctgatccg ggacggccgg
     2281 atcgcgcacc ccgtcaagaa cctccggttc acccagtcga tcctggaggc gctgaccggc
     2341 accgagatga tcgggcgcga gaccgagctc gcgagcgagt tcttcttctc cgccagccgc
     2401 gtccccgcgc tcaaggtctc ctcgttcaac ttcagcgggc gttcggacca ctgaggccgc
     2461 ccggccacgg ttcggggagt cgctgccgct ccgaacgggc aggcgttagg ctgtctcccc
     2521 ccgaccggag gtagccacga tgcaggtccg cgacatcatg cacaccgacg tgaagaccac
     2581 ctcgccctcg gacacgttcg agcaggtggc gaccgtgctg cgcgagcacg ggatctcctc
     2641 ggtcatcgtc atggacggcg acaccctcgc cgg
//
LOCUS       DATBRK010000464         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1579790, whole genome shotgun sequence.
ACCESSION   DATBRK010000464 DATBRK010000000
VERSION     DATBRK010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579790"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VIH70_13500"
     CDS             <1..205
                     /locus_tag="VIH70_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8117423.1"
                     /translation="VETIDGKAGGLAVVIGARIGGLAGASEVLSSQTVRDLTAGSGIV
                     FEDAGEHELKGVPGRWRVYRAAD"
     gene            520..1056
                     /locus_tag="VIH70_13505"
     CDS             520..1056
                     /locus_tag="VIH70_13505"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117424.1"
                     /translation="MKSSTRTARRVWIASAATLIALSACAKSAAPSSGGGSSTSTHAG
                     SIIIGTKSIPGVGTVLVDSNGLTLYGLASESGGTITCTGSCATSWPPLLLPASVSSAT
                     AGSGVSASKLGTISRPDGGTQVTYGGMPLYLFVSDQDPGQATGQGVAGFHVVTPSGSG
                     STSGGSGSSGGRYGYGSG"
BASE COUNT          269 a          499 c          558 g          230 t
ORIGIN      
        1 ggtcgagacg atcgatggca aagccggcgg gcttgcggtc gtgatcgggg cgaggatcgg
       61 cggactcgcg ggggcgtcgg aggtgctcag ctcgcagacg gtgcgagatc tcacggccgg
      121 ctcggggatc gtgttcgagg acgccggcga gcacgagctc aagggcgtcc cggggcggtg
      181 gcgcgtatac cgggccgccg actgatgccc ttgcgacgga cgtcacccga atgggattgc
      241 tgcccgcgga actccacccg attccatcag gcccggcgac gctcaccggg gcaccgagag
      301 agcgatcggc cacagaaagg tggacgtgaa cggggagggc tcgcttccgc cccaagctcc
      361 gcagtctttg accgatgcgt ggagagggca gctcattgaa gatttgcgat tcggtccttg
      421 aattgaaccg gacctgggac ccgaccgtat tccagtcgcg aaaagccagg aagagcgtcg
      481 gccggggctc gagagccccc tgtcctgagg agaccgtagg tgaagtcatc gacaagaacg
      541 gcccgccggg tctggatcgc ctctgcggcc acgctgatcg cgttgtccgc ctgcgccaag
      601 agcgccgccc cgagctcagg cggcggcagt tccacgagta cacacgcggg ctcgatcatc
      661 atcgggacga agtcgattcc cggcgttggc accgtgctcg tggactcgaa tggattgacg
      721 ctctacggat tggcatcgga gagcggcgga acgatcacgt gcaccggctc gtgcgccacg
      781 tcgtggcctc ccttgctcct gccggccagc gtgagctcgg caaccgcggg aagcggagtc
      841 agcgcgagca agctgggcac gatctcgcga cccgacggag ggacgcaggt cacgtacggg
      901 gggatgccgc tgtacttgtt cgtcagcgac caggacccgg gacaggccac cggccagggg
      961 gttgccgggt tccacgtggt caccccctcg ggctccggct caacgagcgg gggaagcggg
     1021 agcagcgggg gccggtacgg gtacgggagc gggtaattcg gggccccaaa ccggtgagcg
     1081 cgatttgacc agaatcgcca ccgcggatga cggccctcgt cccgcggcag gtagcgcatc
     1141 ggcggtggga accgtgggcg ttggccacgc tcgcccggta cagcgcccac cggagccagg
     1201 ccaactccga gcggtcatcc gctacgtcgt ggtcgacctc tatcgcattc gacgggcaag
     1261 ctagagggca ccttgcccaa cggtgaggcg ctcacccgac agcagaaccg acactcgcaa
     1321 atgctgcttg cccgagcgtt ccgagactgc cgacgaggcg gaccggttcc ggtcacctgg
     1381 gcgaaggcca ccgacgccgg tcggcgaacc gttcagcctg ctcatccgta gacccacgga
     1441 gtaccgtgaa tctcgtgcga acggatgagc cgggcagcga cctcgctctg atggcggatt
     1501 tcgcggcccg ccgggagcgt gcggccggcg tgctctacga ccggttcgcg tccagg
//
LOCUS       DATBRK010000465         4015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_406975, whole genome shotgun sequence.
ACCESSION   DATBRK010000465 DATBRK010000000
VERSION     DATBRK010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406975"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..677
                     /locus_tag="VIH70_13510"
     CDS             207..677
                     /locus_tag="VIH70_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117425.1"
                     /translation="MFVRYYLDLPIALEDAEASLLDDPGTWVPGMLEAAENRGEGLLV
                     EVGFALDTRRIDKEIEIALAEPNRLTSKTMLPMTWRATGPERLFPQLDADLEVASLGP
                     RRTQLSISARYRPPLGAFGRALDRAMLHRVAEATIKDFLDRVGERVRARVPSIG"
     gene            1016..1543
                     /locus_tag="VIH70_13515"
     CDS             1016..1543
                     /locus_tag="VIH70_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEY8117426.1"
                     /translation="MKFVVVYESMFGNTEAVAQAIAAGLREAGEVKLGTVDALSPEEV
                     RDAALLVAGGPTHAHGMARRNTHEAIAGERSYRPYGAVQPGHESLRGWIERLTVAPAK
                     AAVFDTRFNKPMLITGSAAKKIAARLERKGYSVIEARSFFVETTGGPLAEGESDRALE
                     WGRELGARARSAIAA"
     gene            complement(1568..2242)
                     /locus_tag="VIH70_13520"
     CDS             complement(1568..2242)
                     /locus_tag="VIH70_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016579326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8117427.1"
                     /translation="MAEPKRPPLRVMLVDDHEVVRDGIRAMLASEDDLHVTAEAGTVR
                     EAIDEADRTRPDVVVMDVRLADGSGIEATREIRAKHPATRVLMLTSFADDEALFASIM
                     AGASGYVLKQVRSGELVSAIRAVGAGDSLLDPAVTSAVLDRLRRGKHLLRDERLARLS
                     PQEERVLTLIADGRTNKEVGDELKLAEKTVKNYVSSILSKLEVARRAEAAAYLARHTT
                     LPGSIE"
     gene            complement(2287..3432)
                     /locus_tag="VIH70_13525"
     CDS             complement(2287..3432)
                     /locus_tag="VIH70_13525"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY8117428.1"
                     /translation="MSDRDAPGDGDRRLIEAGMALASELSLDAVLQRIVELAVDLTDA
                     RYGALGVLTPDGRAIEDFITVGITPEERAALGNPPVGHGLLGALTREPHPIRIPNIAA
                     DPRSVGFPPNHPPMTSLLGARIIGRGTVFGNIYLTNKQDAETFDEEDERVLVILATQA
                     AIAVENARLYDETERKGRELQRLQVFEERERIAKELHDGVIQSLFAVGMNLQGLATSS
                     ADPETARRLEAAVEDVDVAIRDLRNYIFGLRPGILADRQLDQALKELTSEFEARSGVV
                     TVIDVDAEAASSLASRPGEVVQLAREALSNVSRHAEATTCRVSLRRGAEGIVLEIDDD
                     GRGFDVEATTRGMGLRNLRERAESLGGKLEIESTAGEGTTVRATFPS"
     gene            3624..>4015
                     /locus_tag="VIH70_13530"
     CDS             3624..>4015
                     /locus_tag="VIH70_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117429.1"
                     /translation="MDRSSWDRIVSAAGAVVAVVLIVLGAMAIYGGDFGRDNVRDRLV
                     PQHIAFPPADAMTPEERASVGEFAGAKVDTGAEAEAFARYIAGHLEGVNEGATYSETS
                     AAARAEGLDPDVAAELQAKADTLFKGESL"
BASE COUNT          602 a         1345 c         1438 g          630 t
ORIGIN      
        1 aaggcctgga agccttctcc ctcgagcgcg gcagcgagcc gctcgcgttc gatgggattc
       61 ggctccacga ccaagacgtt ccgcgtgtaa cgctccatgg caggcagcat cctcgttcgc
      121 gccatcaccg tcgccggtcg aaggtccctc tatcggcggg tcggatgtcc ctagggacgt
      181 cacggcggag cacgcaccct gatcgcatgt tcgtccgcta ctacctcgac cttcccatcg
      241 ccttggagga cgcagaggcc tcgctgctcg acgatcccgg aacctgggtc ccggggatgc
      301 tggaagccgc cgagaaccgg ggcgagggcc tgctcgtgga ggtcggtttc gctctggata
      361 cgcgtcggat cgacaaggag atcgagatcg ccctcgctga gccgaaccgg ctcacgtcga
      421 agacgatgct cccgatgacc tggcgggcga ccgggccaga gcggttgttc cctcagctcg
      481 acgccgacct agaggtcgcg tcactcggcc cgcggcggac gcagctctcg atcagcgccc
      541 gataccgacc gccgctgggg gcgttcgggc gggcgctgga ccgggcgatg cttcatcgcg
      601 ttgcggaagc cacgatcaag gacttcctgg atcgggtcgg cgagcgcgtt cgcgcacgtg
      661 tgccctcgat cggttaagcg gccacgattc ctcgagcggt agggtgcgcc ccgtgcccga
      721 gaaggcccca ctgctgcgcg tgctactcgt cgataatcac gaggtcgtgc gaagcggcgt
      781 gaaggcgctt ctccaagcca cggaggatgt gctggtgagc gcggaggccg gctcggggcg
      841 tgaggccatc gacgaagccg accgggcacg tcccgacgtc gtcgtgatgg atgttcgcct
      901 cggcgggcgc tcctgggggc actcggcctg catccgggag gaccgttggc cttggcgacg
      961 gggagcatcg cgtcgtgaaa atcgatgtgg aacttggaac cgcaggaggt gcgagatgaa
     1021 gttcgtcgtc gtctacgagt cgatgttcgg caacacggag gcggtcgcgc aggcgatcgc
     1081 cgccgggctc agggaggcgg gcgaggtgaa gctcgggacc gtcgacgccc tgtctccaga
     1141 ggaggtgagg gacgcggcgc tgctcgtcgc gggcggaccg acccatgccc acggtatggc
     1201 gaggcgaaac acgcacgagg ccatcgccgg ggaacggtcg taccgaccgt acggagcggt
     1261 ccaaccggga cacgagagcc tccgtgggtg gatcgagcga ttgacggtcg cccccgctaa
     1321 ggcagccgtg ttcgacacac ggttcaacaa acccatgctg atcactgggt ccgcggcgaa
     1381 gaagatcgcc gcccgattgg agcgcaaggg ctattccgtc atcgaggcac ggagcttctt
     1441 cgtcgagacc acgggtggcc cgctcgccga gggtgagtct gaccgggccc tcgagtgggg
     1501 tcgtgagctg ggcgcccgcg cgaggtcggc gatcgccgcg tagctcggcg gcaggtgagg
     1561 accccgatca ctcgatcgag ccgggtagcg tcgtgtggcg ggcgaggtac gcggccgcct
     1621 cggcccgcct tgccacctcg agcttggaca ggatcgacga gacgtagttc ttgacggtct
     1681 tctcggcgag cttgagctcg tctccgacct ccttgttggt ccggccatcg gcgatgagcg
     1741 tcaggacccg ttcctcctgc ggcgacaatc gggcgagcct ctcgtccctc agcaggtgct
     1801 tccccctgcg gagccggtcc aggacggcgc tcgtcacggc gggatccagg aggctgtcgc
     1861 cggcgccgac ggcgcggatc gcgctgacga gctcgccgga gcgaacctgc ttgaggacgt
     1921 agccggaggc gccggccatg atcgaggcga acagcgcttc gtcgtcggcg aacgaggtga
     1981 gcatcagcac gcgggtagcc ggatgcttcg cgcggatctc ccgcgtcgcc tcgatgccgc
     2041 tcccatcggc caggcggacg tccatcacga cgacatccgg tctggttcgg tcggcctcat
     2101 cgatcgcctc gcggaccgtt ccggcctcgg cggtcacgtg gaggtcgtcc tccgacgcga
     2161 gcatcgctcg gatcccatcc cgcacgacct cgtgatcgtc aacgagcatg acccgcagcg
     2221 gaggacgctt cggctcggcc atccgacgca tggtagtccg gcagggctgg acctcccaga
     2281 gccggctcag gacgggaacg tcgcccggac ggtcgtccct tctccggccg tgctctcgat
     2341 ctccagcttg cctccgaggg actcggctcg ttcccggagg ttgcggaggc ccatcccccg
     2401 ggtcgtcgct tcgacgtcga acccgcgtcc gtcgtcgtcg atctccagga caatgccctc
     2461 tgctcctcgc cgcagcgaga cccggcacgt ggtcgcctcg gcgtgccggc tcacgttcga
     2521 gagggcctcg cgcgcgagct ggaccacctc gccggggcgg gaggccagag aggacgcagc
     2581 ctcggcgtcc acgtcgatga ccgtcacgac gcccgagcgt gcttcgaact ccgaagtcaa
     2641 ttccttgagc gcctggtcga gctgccggtc ggccaggatc cccggccgca gaccgaagat
     2701 gtagttgcgg agatcccgga tcgcgacatc gacgtcctcc accgccgcct cgaggcggcg
     2761 agcggtctcg gggtcggccg aggacgtcgc gaggccctgc agattcatcc cgaccgcgaa
     2821 cagcgactgg atgacgccgt cgtggagctc cttcgcgatc cgctcccgct cctcgaacac
     2881 ctggagccgc tggagctccc tgcccttgcg ttcggtctcg tcgtagaggc gcgcgttctc
     2941 gacggcgatc gccgcctggg tcgcgaggat cacgaggaca cgttcgtcct cctcgtcgaa
     3001 cgtctccgcg tcctgcttgt tggtgaggta gatgttcccg aacacggtcc ctcgcccgat
     3061 gatccgagcc ccgaggagcg acgtcatcgg ggggtggttc ggcgggaacc ccaccgagcg
     3121 ggggtcggcg gcgatattcg ggatccggat ggggtggggc tcacgggtca gggcgccgag
     3181 caggccgtgg ccgaccggcg ggttcccgag ggccgctcgc tcctcgggcg tgatcccgac
     3241 ggtgatgaag tcctcgatcg cccggccgtc cggcgtgagc acgccgagcg ctccgtagcg
     3301 cgcgtccgtg aggtccaccg cgagttccac gatccgctgg aggaccgcgt cgagcgagag
     3361 ctccgaggcg agggccatcc cggcctcgat caggcggcga tccccgtcgc cgggggcgtc
     3421 cctgtcggac atgggccgag cgtagccgaa gctgctcggg cgttccggcc cgccttccgg
     3481 cccgccttcg ggcgtcccgc ccggtcccgg gaggtcccag gtgaactcgg gtccttcggc
     3541 ccgaggcgcg catcggtcgc tggccgacga tgtgcacaac ggggccccat ggagcagggc
     3601 tccaggagcg aaggagcgga gagatggaca ggagcagctg ggacaggatc gtgagcgccg
     3661 ccggtgccgt ggtcgcggtc gtgctcatcg tgctcggcgc gatggcgatc tacggaggcg
     3721 acttcggacg ggacaacgtg cgggaccggt tggtgccgca gcacatcgcg ttcccgccgg
     3781 ccgacgcgat gacccccgag gagcgagcct cggtcggtga gttcgccggc gccaaggtcg
     3841 acaccggcgc cgaggccgag gcgttcgcgc gctacatcgc cgggcacctc gagggcgtca
     3901 acgagggagc gacgtactcg gagacgagcg ccgccgcacg tgcagaggga ctcgacccgg
     3961 acgtggcagc ggagttgcag gcgaaggcgg acaccctgtt caagggcgag tcgct
//
LOCUS       DATBRK010000466         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2735904, whole genome shotgun sequence.
ACCESSION   DATBRK010000466 DATBRK010000000
VERSION     DATBRK010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2735904"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VIH70_13535"
     CDS             <1..603
                     /locus_tag="VIH70_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117430.1"
                     /translation="RVDASSADGSVTSTDVLDVPNEIDPGLNAFVLVVAPGPLKLLGY
                     DASGEIVAHGAVDAAEPAPFPGASLKDGRHFGYVRSVDADARTIEFDLAYFLSGEEAD
                     RAYQEATGDTGPVPNDHFVVNDNPMLRTLALAPDVRLRLLDWNHCCEMFFDGDLSLFA
                     QAIEEQADVTDGDLIYRGQSSWWITIENGVVTEIEEQYSP"
     gene            complement(608..1585)
                     /locus_tag="VIH70_13540"
     CDS             complement(608..1585)
                     /locus_tag="VIH70_13540"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEY8117431.1"
                     /translation="MEIVTKKRMMLFSGTTHRALSEEIAEHLGIPIAPCVIRRFGSGE
                     IYVRPEDSVRGADVFVIQTHADPVNESIMEQLILIDAMKRASAKRITAVVPYYGYARQ
                     DKKVLAREPISAKLVADLLSAAGSDRVVSVDLHAGQIQGYFDFPFDHLTALPVLTQYL
                     RDDLGLHDGDVVVVAPDAGRIKTAERLREYLDADLAYLYKRRSRFEANKIEEMQVVGE
                     VDGRPCILVDDMIDTASTVAKGASVLAQQGAGPIYAAATHPVLSGKAVQNLEEAPIAE
                     VVVTNTMPIPEEKRFGKLRVLSISPLIADALRAVFEESSVSEIFHGENQ"
     gene            complement(1587..>3053)
                     /gene="glmU"
                     /locus_tag="VIH70_13545"
     CDS             complement(1587..>3053)
                     /gene="glmU"
                     /locus_tag="VIH70_13545"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY8117432.1"
                     /translation="KSAVPKVLHPICGRSALWWVLQDVRVARPSKIVIVVHHGAEEVR
                     EAVRSWRIVPEPVFVEQGEALGTGHAVLAAERAAGRASEVLIANGDFDPVAPEDVRTL
                     IRIHRRTKAAATMITTEVERPGRYSRIVRDGDRLASIVEGSDAPASMRSIREVGTNWI
                     VFRRDDLFRALPLVGHENAQGEYYLNDVFPILIEKGERVSVVRVDTGGAMGINSRADL
                     ARTTRVLRERINARHLANGVTLEDPETTYIDADVRIGRDTVIHPMTFLSGETRIATGC
                     EIGPATRIVDTSVGEGAVVEFSVVRSSRIGARAQVGPYAHVRPGTRLGPNAKVGSFVE
                     IKGSEIAEGAKVPHLSYVGDAKIGRRANIGAGTITVNYDGYEKHRTLIGDDARVGSDT
                     MLVAPVKLGKGAVTGAGSVITTDVPAGALALERSEQRVVRGYRKRRDAEAETGKKRGT
                     RVRRTGDTEARAGGSGGSRSERNSASGSPPRSKGKGAH"
BASE COUNT          446 a         1112 c          978 g          517 t
ORIGIN      
        1 cgggtggatg cctcctcggc cgacgggtcg gtcacgtcca cggacgtcct cgacgtgccg
       61 aacgagatcg accccggcct gaacgccttc gtgctcgtcg tcgcacccgg tcctctcaag
      121 ctcctcggct acgacgcgtc cggcgagatc gtcgctcacg gtgccgtcga cgccgcggaa
      181 cccgcgccgt tcccgggtgc ctccctgaag gacggtcgcc acttcgggta cgtgcgctcc
      241 gtcgacgcgg acgcgcgcac gatcgagttc gacctcgcgt acttcctctc cggcgaggag
      301 gccgaccggg cgtaccagga ggccacgggt gacacgggtc ctgtgccgaa cgatcacttc
      361 gtcgtcaacg acaaccccat gcttcgaacg ctcgcgctcg cgcccgacgt gcggctgcgc
      421 ctgctcgact ggaaccactg ctgcgagatg ttcttcgacg gcgacctgtc gctgttcgcc
      481 caggcgatcg aggagcaggc ggacgtgacc gacggcgacc tgatctaccg gggtcagagc
      541 tcgtggtgga tcacgatcga gaacggcgta gtcaccgaga tcgaggagca gtactcgccg
      601 tagtccgcta ctggttctcg ccgtggaaga tctcggagac gctgctctcc tcgaacacgg
      661 ctcggagcgc gtcggcgatc aggggtgaga tcgagagcac cctcagcttg ccaaagcgct
      721 tctcttccgg gatcggcatc gtgttcgtca cgacgacctc ggcgatcggc gcctcctcga
      781 ggttctgcac cgccttgccc gacagcacag ggtgggtcgc ggcggcgtag atcggaccgg
      841 cgccctgctg cgcaagcacg ctcgcgccct tggcgacggt gctcgccgtg tcgatcatgt
      901 cgtcgaccaa gatgcacgga cgcccgtcga cctcgcccac cacctgcatc tcctcgatct
      961 tgttggcctc gaagcgtgat cgacgcttgt acagataggc gagatccgca tccaggtact
     1021 cgcggagacg ctcggcggtc ttgatccggc cggcgtcggg cgcgacgacg accacgtccc
     1081 cgtcgtgcaa cccgaggtcg tctcgcaggt actgcgtcag gaccgggagc gccgtcaggt
     1141 ggtcgaacgg gaagtcgaag tacccctgga tctgacccgc gtgcaaatcg accgagacga
     1201 cccggtccga gccggcggcg ctcaggagat ccgccacgag cttcgcgctg atcggttcgc
     1261 gcgcgaggac cttcttgtcc tgccgcgcgt agccgtagta cgggaccacc gcggtgatcc
     1321 gcttggccga ggcgcgcttc atcgcgtcga tcaggatcag ctgctccatg atcgactcgt
     1381 tcaccgggtc ggcgtgggtc tggatcacga acacgtcggc gccgcgcacc gaatcctcgg
     1441 gacggacgta gatctcgccc gaaccgaacc gtcggatgac gcacggcgcg atcggaatcc
     1501 ccaggtgttc ggcgatctcc tcggacaacg cccgatgcgt cgtgcccgag aacagcatca
     1561 tccgcttctt ggtgacgatc tccacctcag tgcgccccct tgcccttgct tctcgggggg
     1621 ctgccgctcg cgctattgcg ctcgctcctt gagccccccg aaccccccgc ccgcgcttcg
     1681 gtgtcgccgg ttctccgaac cctggtccct cgtttcttgc cggtctcggc ctcggcgtcc
     1741 cgccgcttgc ggtagcccct gaccacgcgt tgctccgaac gctccagggc cagcgcgccc
     1801 gccgggacgt ccgtcgtgat caccgatccc gcgcccgtca ccgccccctt gccgagcttc
     1861 accggcgcaa cgagcatggt atcggaaccc acgcgcgcgt cgtccccgat cagcgtccga
     1921 tgcttctcgt acccgtcgta gttgacggtg atggtcccgg ccccgatgtt ggcacgccgg
     1981 ccgatcttcg cgtcccccac gtaggacagg tgcggcacct tcgcgccctc ggcgatctcg
     2041 gaccccttga tctcgacgaa cgagccgacc ttggcgttcg gccccagccg ggtcccgggg
     2101 cggacgtgcg cgtagggccc aacctgcgcc cgagcgccga tcctcgagga gcgcaccacc
     2161 gagaactcga cgaccgcgcc ctcccccacc gacgtgtcga cgattcgcgt cgccggcccg
     2221 atctcgcacc ccgtcgcgat ccgcgtctcg cccgacagga acgtcatcgg gtggatcacc
     2281 gtgtcgcgtc cgatccggac gtccgcgtcg atgtacgtgg tctccgggtc ttccagggtc
     2341 acgccgttcg cgaggtggcg tgcgttgatc cgctcgcgga gaacccgagt agtcctggcg
     2401 aggtccgcgc gcgagttgat gcccatcgct ccgccggtat cgacccgaac gaccgagacg
     2461 cgctcaccct tctcgatcag gatcgggaag acgtcgttca ggtagtactc gccctgcgcg
     2521 ttctcgtgtc ccacgagcgg cagcgcccgg aacaggtcgt cgcgtcggaa gacgatccag
     2581 ttggtgccca cctcccggat cgatcgcatc gaggcgggcg cgtcgctccc ctcgacgatc
     2641 gacgcgagcc ggtcgccgtc gcgcacgatg cgggagtaac gcccgggacg ctcgacctcg
     2701 gtcgtgatca tcgtggcggc ggctttcgtc cgccggtgga tccggatcaa cgtgcggacg
     2761 tcctcggggg cgaccgggtc gaagtcgccg ttcgcgatca gcacctctga tgcccgcccg
     2821 gccgccctct cggcggcgag gacggcgtgg ccggtcccga gcgcctcgcc ctgctccacg
     2881 aacaccggct cgggaacgat gcgccacgat cggacggcct cgcggacctc ctcggcgccg
     2941 tgatgcacga cgatcacgat cttcgacggg cgcgcgacgc ggacgtcttg cagcacccac
     3001 cacagggcgg accggccgca gatcgggtgc aggaccttgg gcacggccga ctt
//
LOCUS       DATBRK010000467         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1583441, whole genome shotgun sequence.
ACCESSION   DATBRK010000467 DATBRK010000000
VERSION     DATBRK010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583441"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /gene="pilM"
                     /locus_tag="VIH70_13550"
     CDS             <1..785
                     /gene="pilM"
                     /locus_tag="VIH70_13550"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY8117433.1"
                     /translation="AAHKPMVNALVEAALAARLDPQGIDLSPFAVIRAVGTGDLGLDL
                     NSNGDEAIVDIGAQVTSICVHDRGVTRFVRMLPSGGRDITLALASRLGVDDEMAERLK
                     RGERFPGLAVASPPVESPAEEAPAVQLPPPADALPTNTLPTDTLPVEAPRVVPSDPAG
                     VRDLALTRAGSFVDEVRSSLEFYTAQLPNAQIGRVLVIGGGSRLDGLLELMQERLPVP
                     VDRGRLFERAKSEIELSAEASAEAEAVLSVAVGLAIPSRSVS"
     gene            782..1417
                     /locus_tag="VIH70_13555"
     CDS             782..1417
                     /locus_tag="VIH70_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117434.1"
                     /translation="MSEVNLLPPELRERQAIRRTTSLVVAAGLVVLALIGIFYFFQVQ
                     RLSEARSDLEAQQSRNAKLESQIFSLQEFADLQAELASKEALLDEIFVNEVSWSSALL
                     DVSRVIPDASYLTNMSGQITPAAVGEVATQPTGGTPESALIGNMTFAGVANQTETIAT
                     WITRLEDVQGWVNAWVNSAQEDAPFSRIYTFSNGLDLTQEAATARGQGVTP"
     gene            1414..2022
                     /gene="pilO"
                     /locus_tag="VIH70_13560"
     CDS             1414..2022
                     /gene="pilO"
                     /locus_tag="VIH70_13560"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY8117435.1"
                     /translation="MNRRAPIIAGVMAAAVALLAVFLLVLPKMGDVTTAEEELQTAED
                     EEVALAAQFDALQEAQAAAPKTEQKIATIDAQVPPTADLPSLFRLLQGAADRSAVDFF
                     SFTPGTPAPNAGGTYSSIASQVTVSGGYFAIDEFLFLLERLPRAAKVTTLAVTPSGAA
                     GGEITTSTGTLQLLVTVEFYTTDLSAGPGSIPGPSEITGTGA"
BASE COUNT          326 a          753 c          729 g          317 t
ORIGIN      
        1 tggcggcgca caagccgatg gtcaatgccc tggtcgaggc cgccctggcg gccaggctcg
       61 atccccaggg catcgacctg tccccgttcg cggtgatcag ggccgtgggg acgggggacc
      121 tgggcctgga tctgaactcg aacggcgacg aggccatcgt cgatatcggc gcccaggtga
      181 cctcgatctg cgtgcacgac cgcggagtga ccaggttcgt gcggatgctc ccgtccggcg
      241 gtcgagacat caccctcgcg ctcgcgtccc gtttgggcgt cgacgacgag atggccgagc
      301 ggctcaagcg aggcgagcgg ttccccgggc tcgcggtcgc gtcgccgccc gtcgagtccc
      361 ccgccgagga ggcgccggcc gtccagctgc ccccacccgc agacgccctg ccaaccaaca
      421 ccctgcccac cgacacgctg ccggtggagg cgccgcgagt cgtcccctcg gatccggcgg
      481 gggttcgcga cctcgcactc acacgcgcgg ggagcttcgt cgacgaggtt cggtcctcgc
      541 tcgagttcta cacggcgcag ctgccgaacg cgcagatcgg acgcgtgctc gtcatcggag
      601 gaggctcccg actggacggc ctgctcgagc tcatgcagga gcggctgccg gtgccggtcg
      661 accgcggtcg cctgttcgaa cgcgccaagt ccgagatcga gctttccgcg gaggcgtccg
      721 ccgaggccga ggccgtgctg tcggtcgccg tcggcctggc gatccccagc cggagtgtct
      781 cgtgagcgag gtcaacctcc ttccccccga gctccgagag cgccaggcga tccgtcggac
      841 gacgtcgctg gtggtggcgg ccggactcgt ggtactcgcc ctgatcggga tcttctactt
      901 cttccaggtg cagcggctct cggaggcacg gtcggacttg gaggcgcaac agagtcggaa
      961 tgcgaagctc gagagccaga tcttctctct gcaggaattc gcggacctgc aggcggagct
     1021 cgcctcgaag gaagcgctcc tggacgagat cttcgtgaac gaggtgtcct ggtcgagcgc
     1081 gctcctggac gtgtcccggg tgatccccga cgcgtcctac ctgacgaaca tgagcgggca
     1141 gatcaccccg gcggcggtcg gcgaggtcgc cacccagccg acgggcggga cgcccgagtc
     1201 cgcgctgatc ggcaacatga ccttcgcggg cgtggccaac cagaccgaga cgatcgccac
     1261 ctggatcaca cggctcgagg acgtacaggg ctgggtcaac gcctgggtga acagcgccca
     1321 ggaggatgcc cccttctccc ggatctacac cttctccaac gggctcgacc tgacgcagga
     1381 ggcggcgacg gcgcgcggac agggcgtgac gccgtgaacc ggcgcgcgcc catcatcgcc
     1441 ggcgtcatgg cggcggccgt ggcgctcctt gccgtgttcc tgctcgtcct gcccaagatg
     1501 ggcgacgtga ccaccgccga ggaggagctc cagaccgccg aagacgagga ggtcgccctg
     1561 gccgcccagt tcgacgctct tcaggaggcg caagccgcgg cgccgaagac cgaacagaag
     1621 atcgccacga tcgacgcgca ggtgccgcct accgccgacc tcccgagcct gttccgcctg
     1681 ctccaaggag cggccgaccg ctcggccgtc gacttcttct ccttcacacc gggcaccccc
     1741 gcgccgaacg ccggcgggac ctactcgagc atcgcctcgc aagtcacggt cagcggcggg
     1801 tacttcgcga tcgacgagtt cctgttcctg ctcgagaggc tgccgcgggc cgcgaaggtc
     1861 acgacgctcg cggtgacgcc gtcgggggcc gcgggcggcg agatcacgac ctcgaccggc
     1921 accctgcaac tcctggtgac ggtcgagttc tacacgacgg acctgagcgc cgggcccggt
     1981 tcgatcccgg gcccgagcga gatcaccggt acgggagcgt agccatgcag atcacccccc
     2041 gagacaagag gatcctgcag atcgcggggg tcgtggtgcc cctgctgctg ctcgtgtact
     2101 tcttcgtgct gcgaccggac ggcga
//
LOCUS       DATBRK010000468         3942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1299006, whole genome shotgun sequence.
ACCESSION   DATBRK010000468 DATBRK010000000
VERSION     DATBRK010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299006"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VIH70_13565"
     CDS             complement(<1..182)
                     /locus_tag="VIH70_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAA hydrolase family protein"
                     /protein_id="HEY8117436.1"
                     /translation="MRVEARRILLDGVPTTVERDGEELVAPDGGRIALGSAEHLAPTE
                     PTKIVCVHLNYESRVK"
     gene            221..1009
                     /locus_tag="VIH70_13570"
     CDS             221..1009
                     /locus_tag="VIH70_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020127160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEY8117437.1"
                     /translation="MAELKGFVPPLSPEGRSSVVPGPPWHYSGDLLTIEYRAAPGSVE
                     RWLPDPIEPAEEDPDAVAVIFADWQSCSDSFEELLDPVRSQYKEAFVVVRCRYRGETY
                     SRCILIWVDKDFAMVRGWHQGYPKKLGSIWITRPVRHGKAGPHLEPGGRFGASVGVHD
                     RRVVDAGFTITGPSETNGFVNALPMLHSRYFPSIEEGRPPSMDELVTMKSYDWEGSEV
                     WTGDATLSFGASPVEELESLAPVEMIAGYWRSVGVTWGIGTRLA"
     gene            1044..2264
                     /locus_tag="VIH70_13575"
     CDS             1044..2264
                     /locus_tag="VIH70_13575"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY8117438.1"
                     /translation="MTARRLAIAAQRLSGTPPRSSPRAVLGVIRSIRCVQLDPIAVVA
                     RSPYLVLGARVAGFEPKDLERLLWRDRSVYEYWAHAASIVLAEDHPIHAWYMRHYVRD
                     DGSGWNRRRLDWMAANARLRRSVLAQVRRDGPVLARRISGGPTGESWRSSGWTNERNV
                     DRMLEFLWGSGAIMVAGREGIQKLWDLTERVLSPDAPRERLSDLEVTRRAVERSLHGL
                     GVGTPQQIKNHFTRDRYPELKRVLGAFERAGRIERVVVEHDGRTMPGTWYLHRDDVAL
                     LERIERGAWEPRTTMLSPFDNLIADRARSELLFDLPYRMEIYVPKAQRRYGYYAMPVL
                     DGDRFVALVDPAMDRANGRLLVRAVHAAPETKATGDVWRSVAGAVGDLAAWLGATEIE
                     LEGPAPAAFRTALR"
     gene            2308..3075
                     /locus_tag="VIH70_13580"
     CDS             2308..3075
                     /locus_tag="VIH70_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8117439.1"
                     /translation="MRLVRFRFGDRIATGASEFESDTIRVLRGTFFEDPVPTGEEVPF
                     DDVRLLAPVLPSKVVCVGKNYAAHAAEFGSEVPEEPLIFLKPSTAVIGPHDPIPMLPI
                     SRRVDYEGEMAVVIGRIARSVRTEEAFRVILGYTCANDVTLRDRQKTDDQWARAKGFD
                     GSCPLGPWIETDLDPNDVRVETRLNGDVRQAGQTSDMVFGVATLIEYVTTFMTLLPGD
                     VLLTGTPEGVGPVSAGDVVEVEVDGIGTLSNPVEAGS"
     gene            3072..>3942
                     /gene="gltX"
                     /locus_tag="VIH70_13585"
     CDS             3072..>3942
                     /gene="gltX"
                     /locus_tag="VIH70_13585"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY8117440.1"
                     /translation="MNVRTRFAPAPSGSIHVGNARTGLFSWAHARHHGGAFVLRIEDT
                     DASRVTEEAVHGVMESLRWLGIDWDEGPDVGGPHDPYRQSQRMHVYTGTVERLLEQGD
                     AYRCYCTEEELKQRREAAQARGEPSGYDGKCRDPGVRAAFEAEGRPAAIRFEMPEGEH
                     VLHDLVKGEIRWAPGHLRDFVLMRSDGTPVFLLAVAVDDMLMGITHVIRGDDLLESGP
                     RNVAVIEALGGTPPAYAHVPQVHGSDGKPLSKRHGSTSVEAFREQGILPEALMNYLAL
                     LGWSKDADTTFLSK"
BASE COUNT          555 a         1344 c         1450 g          593 t
ORIGIN      
        1 tccttcacgc ggctctcgta gttcaggtgc acgcagacga tcttggtggg ctcggtgggc
       61 gccaggtgtt cggcgctccc gagcgcgatc cggccgccgt cgggggcgac gagctcctcg
      121 ccgtcgcgct cgacggtggt cgggacgccg tcgagcagga tccgccgtgc ctccacacgc
      181 acctccgggt cgttcgagac cgaccgctag tatctcgccc gtggccgagc tgaaggggtt
      241 cgttccgccg ctctcgcccg agggccggtc ctccgtggtg cccggtcctc cgtggcacta
      301 ctcgggcgac ctgctcacga tcgagtaccg ggcggcgccg ggctcggtcg agcgctggct
      361 gccggacccg atcgagccgg ccgaggagga ccccgacgcc gtggccgtga tcttcgccga
      421 ctggcagtcg tgctccgact cgttcgagga gctcctggac cccgtgcgct cccagtacaa
      481 ggaggccttc gtcgtggttc ggtgccggta ccgcggcgag acgtactcgc gctgcatcct
      541 gatctgggtc gacaaggact tcgccatggt ccgcggctgg caccagggct acccgaagaa
      601 gctcggctcg atctggatca cgcgtccggt ccgccacggc aaggccggtc cccacctgga
      661 gcccgggggg cggttcggcg cgagcgtcgg cgtccacgac cggcgggtcg tcgacgccgg
      721 cttcacgatc accggaccct ccgagaccaa cgggttcgtc aacgcgctcc cgatgctgca
      781 ttcgaggtac ttcccgagca tcgaggaggg acgaccgccg tcgatggacg agctcgtcac
      841 gatgaagagc tacgactggg agggctcgga ggtctggacg ggcgacgcca cgctctcgtt
      901 cggcgcgtcg ccggtcgagg agctcgagag cctcgcgccg gtcgagatga tcgccggcta
      961 ctggcgaagc gtcggcgtga cctggggcat cggcacgagg ctcgcctgac cgggtggctc
     1021 gagcgccccg aacgatcgac ggtgtgaccg cgcgccgcct cgcgatcgcg gcgcagcgcc
     1081 tctctggcac cccgccccgt tcgagccccc gggccgtgct cggcgtgatc cgctcgatcc
     1141 ggtgcgtgca gctcgacccg atcgcggtcg tcgcgcggag cccatacctg gtgctcggcg
     1201 cacgggtcgc ggggttcgag cccaaggact tggaacgcct gctctggcgc gaccgctcgg
     1261 tctacgagta ctgggcgcac gcggcctcga tcgtgctcgc cgaggatcac ccgatccacg
     1321 cctggtacat gcggcactac gttcgcgacg acgggtcggg gtggaaccga cggcgcctgg
     1381 actggatggc ggccaacgcg cggctcaggc gctcggtgct cgcccaggtc cgccgggacg
     1441 gccccgtgct cgcgcgtcgg atctccggcg gcccgacggg ggagtcgtgg cgttcgagcg
     1501 gctggaccaa cgagcgcaac gtcgatcgga tgctcgagtt cctgtgggga tcgggtgcga
     1561 tcatggtcgc gggtcgcgag ggcatccaga agctctggga cctgaccgaa cgcgtcctgt
     1621 cgccggacgc gccgcgcgaa cgcctgtcgg acctcgaggt cacgcgtcgc gccgtcgagc
     1681 gctcgctcca cggtctgggg gtcggaacgc cgcagcagat caagaaccac ttcaccaggg
     1741 accggtaccc ggaactgaag cgcgtgctcg gcgcgttcga gcgcgccggt cggatcgaac
     1801 gcgtggtcgt cgagcacgac ggccggacga tgccgggcac ctggtacctg catcgggacg
     1861 acgtggcgct gctcgaacgg atcgagcgcg gcgcctggga gccgcgcacg acgatgctgt
     1921 cgccgttcga caacctgatc gccgatcggg cccgctcgga gctgctgttc gatctcccgt
     1981 acaggatgga gatctacgtc cccaaggccc agcgacggta cgggtactac gcgatgccgg
     2041 tgctcgacgg cgaccggttc gtcgcgctcg tcgaccccgc gatggaccgc gcgaacgggc
     2101 ggttgctcgt gcgggccgtc cacgccgcgc ccgagaccaa ggccaccggc gatgtgtggc
     2161 gttcggtcgc cggggcggtg ggggaccttg ccgcgtggct cggcgcgacc gagatcgagc
     2221 tcgagggtcc cgcgccggcg gcgttccgaa cggcccttcg ctgacccgct cttcgggcgc
     2281 gagcggggcg tgcggtagcc tcgggccgtg aggctcgtcc gcttccgttt cggcgaccgg
     2341 atcgcgaccg gcgcctccga gttcgagtcg gacacgatcc gggtgctgcg gggcaccttc
     2401 ttcgaggatc cggtgccgac gggggaggag gttccgttcg acgacgtgcg gctgctggcg
     2461 cccgtcctgc cctccaaggt cgtctgcgtc ggaaagaact acgcggcgca cgcggccgag
     2521 ttcggatccg aggtacccga ggagccgctg atcttcctca agccctcgac cgcggtgatc
     2581 gggccccacg acccgatccc gatgctgccg atcagccgtc gcgtcgacta cgagggggag
     2641 atggccgtcg tgatcggccg gatcgcgcga agcgtgcgga cagaggaggc cttccgcgtg
     2701 atcctcggct acacgtgcgc gaacgacgtc acgctccgcg accggcagaa gaccgacgac
     2761 cagtgggcgc gcgccaaggg gttcgacggc tcctgcccgc tggggccgtg gatcgagacg
     2821 gacctggatc ccaacgacgt ccgcgtcgag acgcggctga acggcgacgt ccgccaggcg
     2881 ggccagacca gcgacatggt cttcggcgtc gccacgctga tcgagtacgt gacgacgttc
     2941 atgacgctcc tgcccgggga cgtgctcctg acgggaacgc ccgagggcgt gggaccggtg
     3001 agcgccggcg acgtggtcga ggtcgaggtg gacggcatcg ggacgctctc gaacccggtc
     3061 gaggccggct cgtgaacgtc cgaacgcggt tcgcccctgc cccttccggc tcgatccacg
     3121 tcggcaacgc gcggaccggg ctgttctcct gggcgcacgc gcgtcaccac gggggcgcct
     3181 tcgtccttcg gatcgaggac acggacgcct cgcgcgtgac ggaggaggcc gtccacggtg
     3241 tgatggagtc gctgcgctgg ctcgggatcg actgggacga ggggccggac gtcggaggtc
     3301 cgcacgatcc ctaccggcag tcccaacgga tgcacgtcta tacgggcacg gtggagcggc
     3361 tgctggagca gggggacgcc taccgctgct actgcacgga ggaggagctg aagcagcggc
     3421 gcgaggccgc ccaggcccga ggcgagccct ccggctacga cggcaagtgc cgggatccgg
     3481 gggttcgggc cgcgttcgag gccgaggggc gtcccgccgc gatccgcttc gagatgcccg
     3541 aaggcgagca cgtcctgcac gacctggtga agggcgagat ccggtgggcg cccggacatc
     3601 tgcgcgactt cgtgttgatg cgttcggacg gcacgccggt gttcctgctg gccgtcgcgg
     3661 tcgacgacat gctgatgggg atcacgcacg tgatccgagg tgacgacctg ctcgagagcg
     3721 gcccgcgcaa cgtcgcggtg atcgaggcgc tcggcgggac gccgccggcg tacgcgcacg
     3781 tgccgcaggt gcacggctcg gacggcaagc cgctgtctaa gcgccacggc tccaccagcg
     3841 tggaggcgtt ccgcgagcag gggatcctgc ccgaggcgct gatgaactac ctggcgctgc
     3901 tcggctggtc gaaggacgcg gacacgacgt tcctgtccaa ac
//
LOCUS       DATBRK010000469         4047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_843058, whole genome shotgun sequence.
ACCESSION   DATBRK010000469 DATBRK010000000
VERSION     DATBRK010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843058"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1987
                     /locus_tag="VIH70_13590"
     CDS             <1..1987
                     /locus_tag="VIH70_13590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8117441.1"
                     /translation="HPDDREQTLAAHDLHNETGRPWSREYRLIANDGRVVWFRDVATL
                     VRDDDGVPQHWLGVQLDITELKGVEDELRAARDELELRVAERTAELEEANALMALEIG
                     ERRRAEAELRGAEHRYRLLAEQIPAVTYIWEANRSDGAQPQYYTSPRIEQLLGYTVDE
                     WHTPGDFWMSRLHPDDRNAVIAAYLRSESTGEPFAMEFRYLHRDGRIVWVADQAILIE
                     RDDGRPRLFQGVMIDVSARKDAEHAATETELRYRDLAEQVPGIIYILDLTPAEHTFSL
                     RYVSPQLTQILGHQPADWADTERWLATVHPDDLERVRAIDAGIADSSTSEYELEYRMF
                     HQDGSTRWVRDQGHVLARDALGRPTVLQGLVLDVTDARRIDRERREARVRYRSLVEAI
                     PAITYLEHASPGAPEESRFAFVSPQIERILGFTPDEATSDPHFFERTLHPDDLPRIRV
                     ANARAESTGEPFDQEFRQLTKGGEIRWMHDRAVLVRDDDGAPRFWHGVTIDITDRKEA
                     ERNLRLVEARYRTLVEQIPAVTFIETPGAPPDETWFTYLSPQAEEVFGFTSEEVRANP
                     RHLGELVHPEDRERVYAANARAEETGENFNEEFRIVRPDGRIIWLDSRAVLIRDDEGR
                     PRFWQGVAIDVTAHRELESRYRDLAGMVFGELGVDAD"
     gene            complement(1984..3276)
                     /gene="serS"
                     /locus_tag="VIH70_13595"
     CDS             complement(1984..3276)
                     /gene="serS"
                     /locus_tag="VIH70_13595"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY8117442.1"
                     /translation="MLDVRAIREDPDRFRRGLARRHLAEAVDEILELDERRRTLTVRV
                     EELRAEQNRTSKAIGGARGEEKQRLIAEVAKVSAELKELEPRLEQVGAELADLLARTP
                     NVPHESAPDGFTDEDAVEVRRRGELPTFDFEPRDHALLGERLGMLDGERGARTSGSRF
                     VYLLGDIVQVQFALVRHALDLLTARGFVPVIPPVLVREEAMFGSGFLPTDEVNIYVTT
                     PDELYLVGTSEVSLAAFHATEILDEEDLPIRYAGYSTCFRREAGTYGKDMGGMFRVHQ
                     FDKVEMFSFARPEDSWDEHEFMVGIEEEIVGALDLPYRIVNVAAGDLGSAAAKKYDLE
                     VWLPGQGRYRELTSCSNYTDYGARRGQTRVRRSSGEVEVLHTLNGTATAIGRTLIAIL
                     ENYQRADGTVGLPTALHAYLPGHLRELGPSVRPAASTS"
     gene            complement(3278..3685)
                     /locus_tag="VIH70_13600"
     CDS             complement(3278..3685)
                     /locus_tag="VIH70_13600"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY8117443.1"
                     /translation="MADQQGGEVTIVGQGAKLEGTVVSAGSLRIDGQVRGQINAEGDV
                     LLSPQSQVEADIRAQNVVVAGRFKGTILVKGRAELARGGRVDGDITSKTLVVEEGGIF
                     QGQSIMDQQSAPAQKAAAAPAKGATPEAEGAKV"
     gene            complement(3808..>4047)
                     /locus_tag="VIH70_13605"
     CDS             complement(3808..>4047)
                     /locus_tag="VIH70_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEY8117444.1"
                     /translation="GLVRYDAFEDVGGRLSFSCALLDDRGTGVVMTSINGRHDTRVYA
                     KPVAQGHSPYSLSIEEEEAIRQALAPGAERAVSAT"
BASE COUNT          598 a         1502 c         1361 g          586 t
ORIGIN      
        1 gcacccggac gatcgggagc agaccctcgc cgcccacgac ctgcacaacg agaccggccg
       61 gccctggagc cgcgagtacc ggctgatcgc gaacgacggt cgcgtcgtct ggttccgaga
      121 cgtcgccacg ctggtgcgag acgacgacgg cgtgccgcag cactggctgg gcgtccagct
      181 cgacatcacc gagctgaagg gcgtcgaaga cgagctgcgc gccgcccgcg acgagctcga
      241 actccgggtc gccgaacgca ccgccgagct cgaggaggcg aacgccctga tggcgctcga
      301 gatcggtgag cgccggcgcg ccgaggccga acttcggggg gcggagcacc ggtaccggct
      361 cctggccgag cagatccccg ccgtgacgta catctgggag gcgaaccgtt ccgacggcgc
      421 ccagccgcag tactacacga gcccgcgcat cgagcagctc ctcggataca cggtcgacga
      481 atggcacacg ccgggagatt tctggatgtc gcggctgcat cccgacgacc gcaacgccgt
      541 catcgccgcg tacctgcgtt cggagtcgac gggcgagccc ttcgcgatgg agttccgcta
      601 cctccaccgg gacggtcgga tcgtctgggt ggccgaccag gccatcctga tcgagcggga
      661 cgacgggcgt ccccggctct tccagggcgt gatgatcgac gtgtcggcgc ggaaggacgc
      721 cgagcacgcg gcgaccgaga ccgagctgcg ctaccgggat ctcgccgagc aggtcccggg
      781 catcatctac atcctcgatc tcacgcccgc cgagcacacc ttctctctca ggtacgtgag
      841 cccgcagctc acgcagatcc tcggccacca gccggccgac tgggccgaca cggaacggtg
      901 gctcgccacg gtccaccccg acgatctcga gcgcgtccga gcgatcgacg ccggcatcgc
      961 ggactcctcc acgtccgagt acgagctcga gtaccggatg ttccaccagg acgggagcac
     1021 ccggtgggtg cgagaccagg gccacgtgct cgctcgggac gcgctcggtc gccccacggt
     1081 gctgcaggga ctcgtcctgg acgtgacgga cgcacgcagg atcgaccggg agcgccgcga
     1141 ggcccgcgtc cggtaccgct cgctcgtcga ggccatcccg gccatcacct acctcgagca
     1201 cgcgagcccg ggggcgcccg aggagtcccg gttcgccttc gtcagcccgc agatcgagcg
     1261 gatcctcggg ttcaccccgg acgaggcgac gagcgaccca cacttcttcg agcggaccct
     1321 gcacccggac gacctgccgc gcatccgggt ggccaacgcg cgggccgagt cgacgggcga
     1381 gccgttcgac caggagttcc gacagctgac caagggcggc gagatccgtt ggatgcacga
     1441 tcgcgcggtc ctggtccgcg acgacgacgg cgcgccccgg ttctggcacg gggtgacgat
     1501 cgacatcacc gaccgcaagg aggccgagcg caacctccgt ctggtcgagg cgcgctaccg
     1561 cacccttgtc gaacagatcc cggcggtcac gttcatcgag accccggggg cgcccccgga
     1621 cgagacctgg ttcacctacc tgagccccca ggccgaagag gtcttcggct tcacgagcga
     1681 ggaggtccgc gcgaacccgc gccatctcgg cgagctcgta caccccgagg atcgcgaacg
     1741 cgtctacgcc gcgaacgcac gcgccgagga gacgggcgag aacttcaacg aggagttccg
     1801 gatcgtccgc cccgacggcc ggatcatctg gttggacagc cgcgcggtgc tgatccgcga
     1861 cgacgagggc cgtccgaggt tctggcaggg ggtggcgatc gacgtcaccg cccaccgcga
     1921 gctcgagagc cgctaccggg atctggccgg gatggtcttc ggcgagctcg gagtcgacgc
     1981 cgactagctc gtgctcgcgg ccggccggac gctcggcccg agctcgcgga ggtgcccggg
     2041 caggtacgcg tgcagggccg tggggagccc gacggtcccg tccgcgcgct ggtagttctc
     2101 gaggatcgcg atcagggtcc ggccgatcgc ggtggcggtc ccgttgagcg tgtgcaggac
     2161 ctcgacctcg cccgagctgc ggcggacccg ggtctgccct cggcgggcgc cgtagtcggt
     2221 gtagttcgag caggacgtca gctcccgata cctgccctgg cccggcagcc acacctcgag
     2281 gtcgtacttc ttcgccgcgg cgctcccgag gtcgcccgcc gccacgttca cgatgcggta
     2341 cggcaggtcg agggcgccga cgatctcctc ctcgatcccg accatgaact cgtgctcgtc
     2401 ccacgagtcc tccggccgcg cgaacgagaa catctcaacc ttgtcgaact ggtggacgcg
     2461 gaacatcccg cccatgtcct tgccgtaggt gccggcctcg cggcggaagc acgtcgagta
     2521 gccggcgtag cggatcggga ggtcctcctc gtccaggatc tcggtcgcgt ggaacgcggc
     2581 gagcgagacc tccgacgtcc cgaccaggta cagctcgtcg ggagtcgtga cgtagatgtt
     2641 gacctcgtcc gtcgggagga acccgctgcc gaacatcgcc tcttcgcgga ccagcaccgg
     2701 cgggatgacc gggacgaacc cccgcgccgt caacaggtcg agcgcgtggc ggacgagcgc
     2761 gaactggacc tgcacgatgt cgccgagcag gtacacgaat cgggagccgc tggtccgagc
     2821 gccccgttcg ccgtcgagca tgcccagccg ctcacccagc agcgcgtggt ccctgggttc
     2881 gaagtcgaag gtcggcagct cgccgcggcg gcgcacctcg accgcgtcct cgtccgtgaa
     2941 cccgtcgggc gcggattcgt gggggacgtt cggtgtccga gcaagcagat ccgcgagctc
     3001 ggccccgacc tgctccaggc ggggctcgag ctccttgagc tcggccgaga ccttggccac
     3061 ctcggcgatc aggcgctgct tctcctcgcc ccgggcaccg ccgatggcct tgctcgtccg
     3121 attctgctcc gcgcgcagct cctccacgcg gaccgtgagc gtgcgacgtc gctcgtcgag
     3181 ctcgaggatc tcgtcgacgg cctcggccag gtggcgccgg gcgaggcccc tgcggaaccg
     3241 gtcggggtcc tctcggatcg cccggacgtc gagcacgcta gaccttggcc ccctccgcct
     3301 cgggcgtcgc gcccttcgcc ggtgccgcgg ccgccttctg cgccggggcc gattgctgat
     3361 ccatgatcga ctgcccctgg aagatgccgc cctcctcgac gacgagggtc ttcgaggtga
     3421 tgtcgccgtc cacgcgcccg ccgcgcgcga gctcggcccg ccccttgacg aggatcgtgc
     3481 ccttgaacct gccggccacc acgacgttct gcgcgcggat gtcggcctcg acctggctct
     3541 gcggcgagag cagcacgtcg ccctccgcgt tgatctgccc cctcacctgc ccgtcgatcc
     3601 gcagcgaccc ggcggacacg accgtgccct cgagcttggc tccctgcccc acgatggtga
     3661 cctctccgcc ctgctgatct gccatccctc gcacctccgt ccgtcccggt tccgtccggg
     3721 tcgtctcgtc acgctcggcc gaatcggcgt cgcccggcgc cgcggggaga tcgcccgcgg
     3781 cgggtaccgg atcgtcccgt cgccagatca cgtcgccgac accgcccgct cggcgcccgg
     3841 cgccagcgcc tgtcggatcg cttcctcctc ctcgatcgac agactgtagg ggctgtggcc
     3901 ctgcgcgacc ggcttcgcgt acaccctcgt gtcgtgcctg ccgttgatcg aggtcatcac
     3961 cacccccgtg ccacggtcgt cgagcagcgc acaggagaac gacagccggc cgccgacgtc
     4021 ctcgaacgcg tcgtaccgca ccagccc
//
LOCUS       DATBRK010000470         8088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_987516, whole genome shotgun sequence.
ACCESSION   DATBRK010000470 DATBRK010000000
VERSION     DATBRK010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987516"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VIH70_13610"
     CDS             complement(<1..468)
                     /locus_tag="VIH70_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8117445.1"
                     /translation="MQITVKVNGADRGADVEPRMLLAHLIREEFRLTGTHVGCDTTSC
                     GACTVLLDGRPIKSCTMFAVQADGREVTTVEGLLQDGQLHPIQAAFKQEHGLQCGFCT
                     PGMMLTGSALLAENPTPSEDEVRWAISGNICRCTGYMNIVKAIQAAGRDMAAGS"
     gene            complement(499..1365)
                     /locus_tag="VIH70_13615"
     CDS             complement(499..1365)
                     /locus_tag="VIH70_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY8117446.1"
                     /translation="MLPSRFEYHRPDSLEEALHLLAEHGDEAKVLAGGQSLIPVMKLR
                     LAAPAHLVDIGRLGLDAIAETDGRLAIGALARHRDLERSDLVKDRYPVMAAAAPLISD
                     PIVRNLGTIGGSLSHADPAGDWGSVMLALRGSVVLRSERGEREMAVSEFLQDTFTTAL
                     EPDEILTEIRVPQPAGPSGGTYLKLERKVGDFATVAAALHLSLSDGAIDRAGIGLTAV
                     GSKNLQAADAEASLAGAEPTEAAFAEAGRLAAEASSPVTDVRGSAEYKRHVVDVYVRR
                     GLARALEMARAA"
     gene            1493..1951
                     /locus_tag="VIH70_13620"
     CDS             1493..1951
                     /locus_tag="VIH70_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117447.1"
                     /translation="MSGSNRGFWKVVVPLGVGGVLLVVLVLVFRPVARTGAVAYAQAN
                     LRRAAEAAEHLATAGSLADADRETLAVEGGVDDLLLIDPDQSSNDPEVVSVLATRSAW
                     TGSARAETGECFWIRVSAGAGGSFGTVVGTGTDCSADEASAAPPGAWPEP"
     gene            2027..2503
                     /locus_tag="VIH70_13625"
     CDS             2027..2503
                     /locus_tag="VIH70_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8117448.1"
                     /translation="MANVSVTVNGVARQADVEPRTLLVYFLRETLGLTGTNVGCDTTS
                     CGSCTVLVDGESVKSCNLLAVQVDGREVTTIEGLATDGQLHPIQEAFHRNHGLQCGYC
                     TPGMIMAAAGYLRENPNPTDEQVRESLEGNLCRCTGYHNIVRSILDAAGQMSGAKA"
     gene            2514..4880
                     /locus_tag="VIH70_13630"
     CDS             2514..4880
                     /locus_tag="VIH70_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY8117449.1"
                     /translation="MAITEKFVGTSVPRKEDASLITGQGSFVDNLTLPGMLHLAVVRS
                     PFAHARIVNVDVSKALEMPGVVGAWSGADLAGEWAGPLPMVWPITEDIKTSDHWPITK
                     DKARFQGDGVAVVVAESRGRADDAAEVVDVEYEQLDPVLDVEEGLKDGSPLVHDELGT
                     NAVVHWSHGGGGDQGVFDSAPVQLRLRFEAPRITPSPIEPRGAVASVAPAMGEFTLWT
                     STQIPHIARVTLSGTTGIPEHKLRIVAPDVGGAFGAKLNVYAEEALCLALARRTGRPV
                     KWIEDRSENYAVTVHGRGVIHDVEVAATEEGKILGFRVKELCDMGAYFQLLTPGIPEL
                     GGWVYMGPYDTQGYWYEFTGVMTNMAPTDAVRGAGRPEATYVVERAVDTVARHVGKDP
                     AEIRRISFAPRHAEAKPSLMGLQIDSADYLPAFERALELADYEQYRKEQAARREGGEV
                     SQIGIGLSSYIEMSGLAPSNILGALRYAAGGWDAAQVRCQPSGKVTLTIGTSPHGQGH
                     ETTWSQIAADELGVHPDDVEVLHGDTSIAPLGMDTYGSRSISIGGAALHVALQKIKEK
                     ARTLAAHELEVAEDDLDFRDGAFRVQGAPDKVRTIPELAVSAWTAHDLPEGYEPDLTT
                     VATWDPKNFTWPFGTHVCVAEVDTETGFAQLLRYVAVDDCGVVINPMIVDGQVHGGVA
                     HGIAEALYEEVIYDEAGNLVTGTLTQYLVPSAAEMPAFELDRTETPSPTNPLGVKGIG
                     EAGTIAAPPAVINAIVDAVSYLGVTEIGKPATPERVWKAIRDAKGGAA"
     gene            4877..5704
                     /locus_tag="VIH70_13635"
     CDS             4877..5704
                     /locus_tag="VIH70_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY8117450.1"
                     /translation="MIPAPFEYRRVDSVDEAIQALSDGTDAKLLAGGHSLLPLMKLRL
                     ARPSLLVDISRLQDLKYVKRDGDRVAIGALTRHHDVANSEVLHELCPIVSYAAGEIGD
                     PQVRHVGTIGGSVAHADPASDMPSVLLALDAEMVVRGPGGVRSVGAGDFFKGLFEPDL
                     ATDEVLTEIRVPTTTGRGWSYLKFHRRAQDWALVGVAALAQNGAGPSVALTNMADRPV
                     RATGVEEELAGGADPAAASQRAADGTSPPSDAFASAEYRRELVKVLVRRAIEEAQAR"
     gene            5802..6680
                     /locus_tag="VIH70_13640"
     CDS             5802..6680
                     /locus_tag="VIH70_13640"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY8117451.1"
                     /translation="MLMMLAQDRGFGDWFLDVGLPILLTVVAAVLTTLILRVVVGRFQ
                     RKLEQAPHLSEAGNLQRAATLAHALSATFVVVVWVVAGMLILNYLDVPLAPFIASAGI
                     VGVALGFGAQSIVNDTLTGFFILLENQFGVGDIVEIRTPANPILGRVEWLTLRATALR
                     AYDGTLHIVPNGNIVLVSNKSRGWARAIVDTHIAYGQDPEQVRSVLDELFDEFRDELR
                     KDAEMKAWVMDGPSVLGVETTNDRDVVIRVVAETKPSKKVEVERILRARITQRLAERG
                     IRAPVLSAPPPTAPEA"
     gene            6728..7612
                     /locus_tag="VIH70_13645"
     CDS             6728..7612
                     /locus_tag="VIH70_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8117452.1"
                     /translation="MGRMPFSLVESLQEGLAREHYLADRGLSTAIFLAVSLDKPLLLE
                     GEAGVGKTEVAKVLASLLDRELIRLQCYEGIDASQALYEWNYSKQLIAVRAMQDVTAE
                     ANVEDLFGPEYLVERPLLAAIRAGSKAVLLVDELDRADDEFEAFLLEVLSEFAVTIPE
                     IGRVAAEEPPVVIVTSNRTRELHDALKRRCLYHWIDHPALDREIEIIRARAPEVPEAL
                     ARDVASAVSRLRGFDIAKRPGVAETIDWANAVSFLGAEHLDEEIAAATLGTVLKDHDD
                     QQLAVDRLGEVVDGRERP"
     gene            7593..>8088
                     /locus_tag="VIH70_13650"
     CDS             7593..>8088
                     /locus_tag="VIH70_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117453.1"
                     /translation="MVANVPDPGVLERLIGFGRDLRLRGLPVGTGRIVTFCRAAGALG
                     LLERDDLYWAGRSSLISRPEDVEAFDEAFAAWFGTGFRIELPMPGSRPDVDGTGEDER
                     EVEGDGIDDRVIAREWHELEEDALTDGEASIRIVASAAEMLREKSFGDLTEEERLRVS
                     HLIRR"
BASE COUNT         1195 a         2757 c         2967 g         1169 t
ORIGIN      
        1 cgagcccgcc gccatgtcgc ggcctgccgc ctggatcgcc ttgacgatgt tcatgtagcc
       61 ggtgcaccgg cagatgttgc ccgagatcgc ccagcgcacc tcgtcctccg acggcgtggg
      121 gttctcggcg agcagcgcgg agccggtgag catcatgccc ggcgtgcaga agccgcactg
      181 gaggccgtgc tcctgcttga acgcggcctg gatcggatgg agctgcccgt cctggagcag
      241 tccctcgacg gtcgtgacct cccggccgtc ggcctgcacg gcgaacatcg tgcacgactt
      301 gatcggccgg ccgtcgagca gaaccgtgca ggcgccgcag ctggtggtgt cgcagccgac
      361 gtgcgtgccg gtgagccgga actcctctcg gatcaggtgc gcgaggagca tccgaggctc
      421 gacgtcggcg ccgcggtcgg cgccgttgac cttgaccgtg atctgcatcg ttcccctttc
      481 gagccggagt tccgttcctc aggccgcgcg ggccatctcg agcgcccggg cgagaccccg
      541 tcgcacgtac acgtcgacca cgtggcgctt gtactccgcg gatcctcgga cgtccgtgac
      601 cggcgaggag gcctcggcgg ccaggcgccc ggcctcggcg aacgcggcct cggtcggctc
      661 ggcgccggcg aggctcgcct cggcgtccgc cgcctgtagg ttcttggatc cgaccgcggt
      721 caggccgatg ccggcgcgat cgatcgctcc gtccgagagc gagaggtgga gcgcggccgc
      781 gacggtggcg aagtcgccga ccttgcgttc gagcttcagg taggtaccgc cggacgggcc
      841 cgcgggctgg gggacccgga tctcggtcag gatctcgtcc ggctcgagcg ccgtcgtgaa
      901 cgtgtcctgc aggaactcgg agacggccat ctcccgttcg ccgcgctcgc tccgaagcac
      961 cacggagccc cgcaacgcga gcatcaccga cccccagtcg cccgccgggt ccgcgtgact
     1021 gagcgatccg ccgatcgtcc ccaggttgcg gacgatcgga tcggagatga gcggcgcggc
     1081 cgcggccatc accgggtagc ggtccttcac caggtcggat cgctcgaggt cccggtggcg
     1141 cgcgagcgcg ccgatcgcga gacgaccgtc ggtctcggcg atcgcgtcca ggccgagccg
     1201 accgatgtcg accaggtgcg cgggcgccgc caaccgcagt ttcatgaccg ggatcaggct
     1261 ctgcccgccg gccagcacct tcgcctcatc gccgtgctcg gcgagcaggt gcagggcctc
     1321 ttcgagcgag tccggtcggt ggtactcgaa cctcgaaggc agcatgggga acctcctcgc
     1381 tcggagccgt cgcgcgcaag gtccaggcgc cgcaacgacg gtgacacgac tatccctccg
     1441 ggcctcggga acggtcaagt cagtgcgcga acgccctggg tatcgtccgg ccgtgtccgg
     1501 ctccaatcgc ggcttctgga aggtggtcgt cccgctcggc gtcggcgggg tcctgctggt
     1561 cgtgctggtg ctggtgttcc gcccggtcgc ccgcacgggc gccgtcgcgt acgcgcaggc
     1621 gaacctccga agggcggccg aggccgccga gcacctggcc acggccggct cgctcgcgga
     1681 cgcggatcgc gaaaccctcg cggtcgaggg cggggtcgac gacctgctcc tgatcgaccc
     1741 ggaccagagc tcgaacgatc ccgaggtggt cagcgtgctc gcgaccaggt cggcgtggac
     1801 gggctcggcc cgggccgaga ccggggagtg cttctggatc cgcgtctccg ccggtgccgg
     1861 cggatcgttc gggaccgtgg tcggcaccgg aacagactgc tcggccgacg aggcgagcgc
     1921 ggccccgccg ggggcctggc ccgagcccta gttgctccca cacggactcg cgcggccgaa
     1981 cgtttcgggc ttacgatcgg tgttcacccg ttgcaaaggg ggacccgtgg ccaacgtgtc
     2041 ggtcaccgtc aacggggtcg cgcgccaggc ggacgtcgag ccgcgcacgc tcctcgtgta
     2101 tttcctccgg gagaccctcg ggctcaccgg gaccaacgtc ggatgcgaca ccacgtcgtg
     2161 cggctcgtgc accgtcctgg tggacggcga gtccgtcaag tcgtgcaacc tgctcgcggt
     2221 gcaggtcgac gggcgggagg tcacgacgat cgaggggctg gccaccgacg gtcagttgca
     2281 tccgatccag gaggcgttcc atcgcaacca cggcctccag tgcggctact gcacgccggg
     2341 catgatcatg gcggcggccg gctacctgcg ggagaacccg aacccgaccg acgagcaggt
     2401 gcgcgagtcg ctcgagggca acctctgccg ctgcaccggc taccacaaca tcgtgaggtc
     2461 gatcctcgat gcggccggtc agatgtccgg cgcgaaggcc tgaggggggc gagatggcga
     2521 tcaccgagaa gttcgtcgga acgtcggtcc cccgcaagga agacgcctcg ctcatcacgg
     2581 ggcagggcag cttcgtcgac aacctgacgc tccccggcat gctccacctg gcggtcgtgc
     2641 ggagcccgtt cgcccacgcg cggatcgtga acgtcgacgt ctcgaaggcg ctcgagatgc
     2701 cgggcgtggt cggcgcctgg agcggcgcgg acctggccgg cgagtgggcc ggcccgctgc
     2761 cgatggtctg gccgatcacc gaggacatca agacctcgga tcactggccg atcacgaagg
     2821 acaaggcgcg gttccagggc gacggcgtgg ccgtcgtcgt cgccgagtcg cgcggccggg
     2881 cggacgatgc ggccgaggtc gtcgacgtcg agtacgagca gctcgatccg gtcctcgacg
     2941 tcgaggaggg gttgaaggac ggctcgccgc tcgtgcacga cgagctcggg accaacgccg
     3001 tggtgcactg gagccacggc ggcggcggcg accagggcgt gttcgacagc gcgcccgtgc
     3061 agctgcggct ccggttcgag gcgccgagga tcacgccgag cccgatcgag ccgcgcggtg
     3121 cggtcgcgtc ggtggcgccg gcgatgggcg agttcacgct gtggacgtcg acgcagatcc
     3181 cgcacatcgc ccgggtcacg ttgtcgggca cgaccgggat ccccgagcac aagcttcgga
     3241 tcgtcgcccc cgacgtgggc ggggcgttcg gcgccaagct gaacgtctac gcggaggagg
     3301 cgctctgcct cgcgctcgcg cgcaggaccg gtcgcccggt caagtggatc gaggaccgct
     3361 cggagaacta cgccgtaacc gtccacggac gcggggtgat ccacgacgtc gaggtcgccg
     3421 cgaccgagga ggggaagatc ctcgggttcc gggtgaagga gctgtgcgac atgggcgcgt
     3481 acttccagct cctgacgccg gggatccccg agctcggggg ctgggtctac atgggcccct
     3541 acgacacgca ggggtactgg tacgagttca cgggcgtcat gacgaacatg gcgcccaccg
     3601 acgccgtccg gggagccgga cggccggagg cgacctacgt cgtcgaacgc gcggtggaca
     3661 ccgtggcgcg gcacgtcggc aaggacccgg ccgagatccg acggatcagc ttcgcgccga
     3721 ggcacgcgga ggcgaaaccg tcgctgatgg ggttgcagat cgactcggcg gactacctcc
     3781 cggcgttcga gcgcgcgctc gagctcgcgg actacgagca gtaccggaag gagcaggcgg
     3841 ctcgccgcga gggcggggag gtgagccaga tcgggatcgg gctctccagc tacatcgaga
     3901 tgtccgggct ggcgccgtcg aacatccttg gcgcgctccg gtacgcggcg ggcggctggg
     3961 acgcggcgca ggtgcggtgc caaccgagcg gcaaggtcac gctcacgatc ggcacctcgc
     4021 cgcacggcca gggccacgag accacctggt cccagatcgc ggccgacgag ctgggtgtgc
     4081 accccgacga cgtggaggtc ctgcacggcg acacgtcgat cgccccgctc ggcatggaca
     4141 cctacggatc gcgctcgatc tcgatcggcg gcgcggcgct gcacgtcgca ttgcagaaga
     4201 tcaaggagaa ggcgcggacg ctggcggcgc acgagctcga ggtggccgag gatgatctgg
     4261 atttccggga cggggcgttc cgtgtgcagg gcgcgcccga caaggtccga acgatccccg
     4321 agctggccgt gtccgcgtgg acggcgcacg acctgcccga ggggtacgag cccgatctca
     4381 ccacggtcgc gacctgggac ccgaagaact tcacctggcc gttcgggacc cacgtctgcg
     4441 tcgccgaggt cgacaccgag acgggatttg cccagctgct ccgctacgtg gcggtcgacg
     4501 actgcggggt cgtgatcaac ccgatgatcg tcgacggaca ggtgcacggc ggtgtcgctc
     4561 acgggatcgc ggaggccctc tacgaggagg tcatctacga cgaggcgggc aacctggtca
     4621 cggggacgct gacgcagtac ctcgtcccct cggccgcgga gatgccggcg ttcgagctcg
     4681 accggaccga gacgccctcg cccaccaacc cgctcggcgt caaggggatc ggcgaggccg
     4741 gcacgatcgc ggccccgccg gcggtgatca acgcgatcgt ggacgcggtg tcgtacctcg
     4801 gcgtgaccga gatcggcaag ccggcgacgc ccgagcgcgt gtggaaggcg atccgcgacg
     4861 cgaagggagg tgcggcatga tcccggcgcc cttcgagtac cggcgcgtgg actcggtcga
     4921 cgaagcgatc caggcgctgt ccgacggaac ggacgcgaag ctcctcgcgg gcggtcactc
     4981 gctcctgccg ttgatgaagc tccgcctggc gaggccttcg ctgctcgtgg acatctcgcg
     5041 gctgcaggac ctgaagtacg tgaagcggga cggagaccgg gtcgcgatcg gcgcgctcac
     5101 gcggcaccac gacgtggcga acagcgaggt cctgcacgag ctctgcccca tcgtgtcgta
     5161 cgcggccggc gagatcggcg acccgcaggt ccgccacgtc gggacgatcg gtggttcggt
     5221 ggcccacgcc gacccggcct cggacatgcc gagcgtgctc ctggccctcg acgccgagat
     5281 ggtcgtccgg ggccccgggg gcgttcggag cgtcggggcg ggcgacttct tcaaggggct
     5341 gttcgaaccc gaccttgcga ccgatgaggt cctgaccgag atccgcgtcc ccacgacgac
     5401 gggtcgcggc tggagctacc tgaagttcca ccgccgggcg caggactggg cgctcgtggg
     5461 tgtcgccgcg ctcgcgcaga acggcgcggg gccctcggtc gccctcacga acatggcgga
     5521 ccgacccgtg cgggcgacgg gcgtggagga ggagctggcc ggcggcgccg atccggccgc
     5581 ggcgtcgcag cgcgcggccg atggcacgag cccgccgagc gacgcgttcg cgagcgccga
     5641 gtaccgccgg gagctcgtga aggtgctcgt ccgccgggcg atcgaggagg cgcaggcacg
     5701 atagacgtcg gggagacgcc ccgcacggca gaccgaggcc gggcgcgtcg cgcccggcct
     5761 cggtctttga cgctcgcggg gcggtctgcc tagatgggcg gatgctgatg atgctcgcgc
     5821 aggaccgcgg gttcggggat tggttcctcg acgtgggcct gccgatcctt ctgacggtcg
     5881 tggctgcggt gctcacgacg ctgatcctgc gggtggtcgt cggccggttc cagcgtaagc
     5941 tcgagcaagc gccgcacctg tccgaggcag gcaacctgca gcgtgctgcc accctcgcgc
     6001 atgcgctctc cgccacgttc gtcgtggtcg tgtgggtggt cgccgggatg ctgatcctca
     6061 actacctgga tgtgcctctc gccccgttca tcgcgagcgc cgggatcgtg ggcgtggcgc
     6121 tggggttcgg cgcgcagagc atcgtcaacg acacgctgac cgggttcttc atcctgctcg
     6181 agaaccagtt cggcgtcggc gatatcgtgg agatccgcac gcccgcgaac cccatcttgg
     6241 gacgggtcga gtggctcacg ctgcgggcga ccgcgctccg cgcgtacgac gggacgctgc
     6301 acatcgtgcc gaacggcaac atcgtgctcg tgagcaacaa gtcgcgaggg tgggcccgcg
     6361 cgatcgtcga cacgcacatc gcgtacggcc aggacccgga acaggtgcgg agtgtcctcg
     6421 acgagctgtt cgacgagttc cgggacgagc tgcggaagga cgccgagatg aaggcgtggg
     6481 tcatggacgg gccctcggtg ctcggcgtcg agacgacgaa cgatcgcgat gtcgtgatca
     6541 gggtcgtcgc cgagacgaag ccgtcgaaga aggtcgaggt cgaacgcatc ctgcgcgccc
     6601 gcatcaccca gcgtctggcc gaacgcggga tccgggcccc cgtcctctcc gcccccccgc
     6661 cgaccgctcc cgaagcctag gctcccggac cttcgccggg ccgagcggcc ggtaggcttt
     6721 cggcacgatg ggtcggatgc cgttcagcct ggtcgagtcg ctccaggaag ggctcgcgcg
     6781 cgagcactac ctggcggacc gggggctgtc cacggcgatc ttcctcgcgg tctcgctcga
     6841 caagccgctg ctgctcgagg gtgaggccgg ggtgggcaag accgaggtcg cgaaggtgct
     6901 cgcgtcgctc ctggatcgcg agctgatccg gctgcagtgc tacgagggca tcgatgccag
     6961 ccaggcgctg tacgaatgga actactccaa gcagctgatc gcggtccgcg cgatgcagga
     7021 cgtgaccgcc gaggcaaacg tcgaggacct gttcggcccc gagtacctgg tcgaacgccc
     7081 gctcctggcg gcgatccgcg ccggttcgaa agccgtcctg ctcgtcgacg agctcgaccg
     7141 cgcggacgac gagttcgagg cgttcctgct cgaggtgctg tccgagttcg ccgtcacgat
     7201 ccccgagatc ggccgcgtgg ccgcggagga gccgccggtc gtcatcgtga cctcgaaccg
     7261 cacgcgcgag ctccacgacg cgctcaagcg ccggtgcctg taccactgga tcgaccaccc
     7321 cgcgctcgac cgggagatcg agatcatccg agcccgcgcg cccgaggtcc ccgaggccct
     7381 cgctcgcgac gtcgcgtccg ccgtgagccg gctccgggga ttcgacatcg ccaagcgccc
     7441 cggcgtggcc gagacgatcg actgggcgaa cgcggtctcg ttcctcggcg ccgagcacct
     7501 ggacgaggag atcgcggcgg cgacgctcgg caccgtgctg aaggaccacg acgatcagca
     7561 gctcgcggtc gaccgcctgg gcgaggtcgt cgatggtcgc gaacgtccct gaccccggcg
     7621 tcctcgagcg cctgatcggg ttcggccgcg acctgcggct ccggggtctg ccagtcggca
     7681 ccggacgcat cgtcacgttc tgccgcgcgg ccggcgcgct cggcctcctc gaacgcgacg
     7741 acctgtattg ggccggccgg tcgtcgctga tctcgcgccc ggaggacgtc gaggcgttcg
     7801 atgaggcgtt cgccgcctgg ttcgggacgg ggttccgcat cgagcttccg atgcccggct
     7861 cgcggcccga cgtcgacggg acgggcgagg acgaacgcga ggtcgagggc gacgggatcg
     7921 acgatcgcgt gatcgcgcgc gaatggcacg agctcgagga ggacgcgctc accgacggcg
     7981 aggcgtcgat ccggatcgtg gcgagcgccg ccgagatgct tcgggagaag tcgttcggcg
     8041 acctgacgga ggaggagcgc ctgcgcgtgt cgcacctgat ccgcaggc
//
LOCUS       DATBRK010000471         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_843414, whole genome shotgun sequence.
ACCESSION   DATBRK010000471 DATBRK010000000
VERSION     DATBRK010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843414"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VIH70_13655"
     CDS             complement(<1..542)
                     /locus_tag="VIH70_13655"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8117454.1"
                     /translation="MRAAVLWDIDDLRIEDLDLDPPKAGEVEIRMAASGVCRSDFHSV
                     HGIHRHAMPIVLGHEGSAVVEEVGEGVTGVKPGDHVVCSWLPYCGSCRRCAAGRPAVC
                     ERLAVFDEGFLADGTTRFSHTGTRIHHNVPSSFAERSVVPANTVFPVDPSLPLEQVAL
                     LGCAVMTGVGAVLNTAKVRPG"
     gene            complement(576..1046)
                     /locus_tag="VIH70_13660"
     CDS             complement(576..1046)
                     /locus_tag="VIH70_13660"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8117455.1"
                     /translation="MDPGGVMAYHWRMHIDAPPERVFDTLADVEHHPEWANPSAKLKM
                     SSVSGGPPRIGSTYRSEQVFVGKPQTAEIKIVEFDRPNRFAFAVTQRKQGSTKDVRYR
                     HTFVLTPEGRGTRLERTTASVGGNALLDAVVTPAVKADGKKSLANLKRKLEAPA"
     gene            1193..1777
                     /locus_tag="VIH70_13665"
     CDS             1193..1777
                     /locus_tag="VIH70_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117456.1"
                     /translation="MDGWIWIVIGAVIVVLVVAVVAAYVLRQQRRRAELRETFGAEYE
                     LAVAREGDVRRGESDLIARRKRRDKLDIRPLSPSSRDEYARTWEQTQARFVDDPADAL
                     RRADTLIIAVMRERGYPMDDFEQRADDISVDHPDVVQHYRSAHAVSTRVDGHRDESTG
                     TNVSTEDMRLGLVHYRALFQQLLEPEASERQAIG"
     gene            1939..2985
                     /locus_tag="VIH70_13670"
     CDS             1939..2985
                     /locus_tag="VIH70_13670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019667664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY8117457.1"
                     /translation="MPRRYLLHLDLDEFIAAVERLRHPELEGMPIVVGGDGDPSKRGV
                     VSTASYEARAFGIRSAMPLRTAYRRNPDAIFLPVDSSAYLAASGEVMDTLRTFDAEVE
                     VAGWDEAFLAVTTDDPETFAKRIQERVLERTRLWCTIGIGDNRLQAKLASGFGKPRGV
                     FVLTSDRWDESMHGLATTELWGIGSKTAAKLAGLGIRTVGQLAAADEGTLAERFGPST
                     GPWLASLGRGEGSASVHPGGWTAKGRSREHTFQRNLTDPNQVGAEVARTAREVASDLR
                     RDDRLAARVVVKVRFAPFFTSTHGVTLAEPTADADLVAEAAARALGRFELDRPVRLVG
                     VRAEMTPPVVQDGA"
     gene            2982..>3137
                     /locus_tag="VIH70_13675"
     CDS             2982..>3137
                     /locus_tag="VIH70_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011762224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEY8117458.1"
                     /translation="MSVQYHLKLAPGDVAPYVLLPGDPGRVPLVASLWDESREVASNR
                     EYVTFTGT"
BASE COUNT          469 a         1101 c         1103 g          464 t
ORIGIN      
        1 cccgggcgca ccttcgccgt gttcagcacc gcgccgacgc ccgtcatgac cgcgcatccg
       61 aggagggcga cctgttcgag cggcaacgac ggatcgacgg gaaacaccgt gttcgccggt
      121 accaccgatc gctcggcgaa cgaggacggc acgttgtggt ggatccgcgt ccccgtgtgc
      181 gagaagcggg tcgtcccgtc ggccaggaac ccctcgtcga agaccgccag ccgttcgcac
      241 acagccggac gtcccgccgc gcatcggcgg cacgagccgc agtacggcaa ccacgagcag
      301 acgacgtggt cgccgggctt cacgccggtg acgccctccc ccacctcctc gaccacggcc
      361 gagccctcgt gcccgagcac gatcggcatc gcgtgccggt ggatgccgtg gaccgagtgg
      421 aagtccgagc ggcacacgcc cgaggccgcc atccggatct cgacctcccc ggccttgggc
      481 gggtccaggt cgaggtcctc gatgcggagg tcgtcgatgt cccagagcac ggccgctcgc
      541 atgcgcggag tatcgcgcac gggcgccgcc gatggtcagg ccggagcttc gagcttcctc
      601 ttcaggttcg cgagcgactt cttcccgtcg gccttgaccg ccggggtcac gacggcgtcg
      661 agcagcgcgt tcccgccaac gctggcggtc gtgcgttcga gcctggtccc ccggccctcc
      721 ggcgtcagca cgaacgtgtg ccggtaccgc acgtccttcg tcgagccctg tttgcgttgg
      781 gtgacggcga acgcgaagcg gttcgggcgg tcgaactcca cgatcttgat ctcggccgtc
      841 tgcggcttgc cgacgaagac ctgctcggag cggtaggtcg agccgatccg gggcgggcct
      901 ccggagaccg aggacatctt cagcttggcg ctggggttgg cccactcggg gtggtgttcg
      961 acgtcggcca gcgtgtcgaa caccctctcc ggtggggcat cgatgtgcat cctccagtgg
     1021 taggccatca cgcctcccgg gtccacggcg atcgctcggc gtccggagcg gggcgctatt
     1081 tcagcatggc cttacgtaag cgtcaactga agattcgccg ccgcttcgcg gggggccgcg
     1141 atgggtatca gtcctccgtg gcaagtggat cagttcggag cgaggaggag ccatggacgg
     1201 ctggatatgg atcgtgatcg gcgcggtcat cgtcgtgctc gtcgtggccg tcgtggccgc
     1261 gtacgtcctc cgacagcagc gccgccgcgc ggaactgcgg gagaccttcg gcgcggaata
     1321 cgagctcgcc gtcgcgcgtg agggcgacgt gcggcggggc gagtccgacc tgatcgctcg
     1381 gcgcaagcgc cgagacaagc tcgacatcag gccattgtcc ccctcatcga gggacgagta
     1441 cgcgcggacc tgggaacaga cccaggctcg cttcgtcgac gaccccgcgg acgcgctccg
     1501 tcgagcggac acgctcatca tcgccgtgat gcgggagcgg ggatacccga tggacgattt
     1561 cgagcaacgt gccgacgaca tctccgtcga ccacccggac gtcgtgcagc actatcgctc
     1621 tgcgcacgcc gtctcgacac gtgtcgacgg ccatcgggac gagtcgaccg gaacaaacgt
     1681 gtcgaccgag gacatgcgcc tggggttggt gcactaccga gcgcttttcc aacagctcct
     1741 cgaacccgag gcctcggagc gtcaggcgat cggctgagcc gaggactccg atcgcacagg
     1801 gagacctccg tccctcgtag gcctcgtagg ccggcgtcgg ggttctgtcg tcacggagtc
     1861 gcggtttcgt gacagaaccc tgacgccggg gcgttgcgac cccaacgact tcccacgccc
     1921 gtttgcgatg ctggcgccgt gcccaggcgc tacctgctcc atctggacct cgacgagttc
     1981 atcgccgccg tcgaacggct ccggcacccc gaactcgagg gcatgccgat cgtcgtcggc
     2041 ggcgacggcg acccgagcaa gcgcggggtg gtctcgaccg cgagctacga ggcgcgggcg
     2101 ttcgggatcc ggagcgcgat gccgctccgc accgcgtaca ggcgcaaccc cgacgcgatc
     2161 ttcctccccg tcgactcgag cgcgtacctc gccgccagcg gcgaggtcat ggacacgctc
     2221 cgtacgttcg acgccgaggt cgaggtcgcg gggtgggacg aggcgttcct ggccgtcacg
     2281 acggacgacc cggagacgtt cgcgaagcgg atccaggagc gggtgctcga gcgcacgcgc
     2341 ctgtggtgca cgatcgggat cggcgacaac cgcctgcagg caaagctcgc gagcgggttc
     2401 ggcaagccgc gcggcgtgtt cgtgctcacc tcggaccgat gggacgagtc catgcacggg
     2461 ctcgccacca ccgagctgtg gggcatcggg tccaagacgg cggccaagct cgccgggctg
     2521 gggatccgca cggtgggcca gctcgcggcc gcggacgagg gcacgctcgc cgaacgcttc
     2581 ggcccgtcga cggggccgtg gctcgcgagc ctcggtcgag gcgagggctc ggcgagcgtg
     2641 caccccgggg gctggaccgc caagggccgg agccgggagc acacgttcca gcgcaacctc
     2701 acggatccga accaggtcgg cgccgaggtc gcccgcaccg cgcgcgaggt cgcgagcgat
     2761 ctccgcaggg acgaccggct cgccgcacgc gtggtggtca aggttcggtt cgcgccgttc
     2821 ttcaccagca cccacggcgt cacgctcgcc gagcccacgg cggacgccga cctcgtggcc
     2881 gaggccgccg ctcgcgcgct cgggcgcttc gagctcgacc ggcccgtccg cctcgtcggg
     2941 gtccgcgcgg agatgacgcc tccggtggta caagacggcg catgagcgtc cagtatcacc
     3001 tgaagctcgc gccgggcgac gtcgcgccgt acgtcctgct cccgggcgac cccggcaggg
     3061 tcccgctcgt cgccagcctg tgggacgagt cgcgcgaggt cgcgagcaac cgcgagtacg
     3121 tgacgttcac cggcacg
//
LOCUS       DATBRK010000472         4010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1726362, whole genome shotgun sequence.
ACCESSION   DATBRK010000472 DATBRK010000000
VERSION     DATBRK010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726362"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VIH70_13680"
     CDS             complement(<1..311)
                     /locus_tag="VIH70_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117459.1"
                     /translation="MFTEERMIVREGARYWWVFLLTGVAWLLIAWLVLRMDITSIAAV
                     GVLLGVVFLAASVNEVGLGSLMSGGWKILHYVMALIFFIGGLWSLVRPVDSFFALASV
                     LG"
     gene            complement(459..980)
                     /locus_tag="VIH70_13685"
     CDS             complement(459..980)
                     /locus_tag="VIH70_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117460.1"
                     /translation="MALDTFTLIASQYALEEDALEDYDAVRAAYEDLGIIDTYDAAVV
                     TKGPDGKVRIVERIEEPTRHGAIAGLAVGLAVGALVALFPAVALGAGLAIGGASGAAV
                     GATAGHVAAGMSRSDLKDLGELLDAGTSGLIVVAATDVEGRVEAAIQRGKDIVKKQVQ
                     IDADGLKTEIDAL"
     gene            complement(1333..2529)
                     /locus_tag="VIH70_13690"
     CDS             complement(1333..2529)
                     /locus_tag="VIH70_13690"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8117461.1"
                     /translation="MSTPDPKAVADEVTPTGAAEPSISTSMPTWFPRALRLTIGYVLG
                     TLALLWFLGQAHDLLGLVLLAAFFSLSLEPAVNRLHRRGWSRGAATATLLLGVMAFFL
                     ILGLMVIPIMVENIGKMADDVPGWIDDVNGYTQREFDVQLIPDTAIHPSQSSVGLAKN
                     FVSDYGGALVGAVGGLVGAIFAVFTIALFAFYMTSKAPRIRRSVVSLLRPEHQRDVLW
                     AWNVALEKTGGYLYQRILLAVINSSLMLIVMLLAGIPFAVPLAIFTGFTAAFIPIVGT
                     YLAGIVPVVVALAAAGPGRAIILLAWIVIYQQLENYLLEPRLSQQTMNLNPGIALGAA
                     LAGGSVGGLIGAFFALPTAAAIQAFMTTYATRYEVEDTELTRVDEAAQRAAGSPPPAE
                     EGGPGK"
     gene            complement(2674..2985)
                     /locus_tag="VIH70_13695"
     CDS             complement(2674..2985)
                     /locus_tag="VIH70_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117462.1"
                     /translation="MSQDCLLCRAERVTDWHFEDEECWVTDCVVCRTPMIVWRPHGLP
                     EPELERRLLAHLERIAAERYPDGFWIDPERRRIPDHWHAHARPANGFFDPTSDLYGRF
                     G"
     gene            complement(2996..3451)
                     /locus_tag="VIH70_13700"
     CDS             complement(2996..3451)
                     /locus_tag="VIH70_13700"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY8117463.1"
                     /translation="MRLAPLLLLVVVACGSAEQAPDPHASAILSIGTDDGTVSLDVQV
                     ADTQAERQTGLMGRESLSPYDGMAFVWKEPVVSTFWMKDTLIPLSIAFWDDHDRIISI
                     LAMDPCTEEPCPSYGPDEPFVGAVEVARGTFERHGVAPGDRVELTVSNG"
     gene            3580..3652
                     /locus_tag="VIH70_13705"
     tRNA            3580..3652
                     /locus_tag="VIH70_13705"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3613..3615,aa:Thr,seq:ggt)
     gene            3733..3804
                     /locus_tag="VIH70_13710"
     tRNA            3733..3804
                     /locus_tag="VIH70_13710"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3765..3767,aa:Met,seq:cat)
     gene            3891..>4010
                     /gene="rpmG"
                     /locus_tag="VIH70_13715"
     CDS             3891..>4010
                     /gene="rpmG"
                     /locus_tag="VIH70_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY8117464.1"
                     /translation="MAKGDNRPTITLACTECKQRNYVTVKNRVNDRDRIELRKY"
BASE COUNT          713 a         1354 c         1360 g          583 t
ORIGIN      
        1 ccgagcaccg aggcgagcgc gaagaagctg tcgaccggcc ggacgagcga ccacagcccc
       61 ccgatgaaga agatgagcgc catcacgtag tgcaggatct tccagcctcc ggacatcagg
      121 ctccccagcc cgacctcgtt gacgctcgcg gcgaggaaga ccaccccgag cagcacgccg
      181 accgcggcga tcgacgtgat atccatgcgc agcaccagcc aggcgatcag cagccatgcg
      241 acccccgtca acaggaacac ccaccagtaa cgggccccct cccggacgat catgcgttcc
      301 tccgtgaaca tcgtccccct cctcctcgct cccgcgggtc tgtccacgcg ggccggcctg
      361 accgtatgtc ctccggggca acggaatcaa actcgctcgg cggccttggg cagggggctg
      421 gccggcccca gccacgagac gccagacgga tccgtcgatc agagcgcgtc gatctcggtc
      481 ttgaggccgt ccgcgtcgat ctgcacctgc ttcttgacga tgtccttccc ccgctggatc
      541 gccgcctcga cgcgtccctc aacatcggta gcagcgacca cgatgagccc gctggtgccg
      601 gcgtcgagca actcgccgag atccttgaga tcggagcgac tcatcccggc agcgacgtgt
      661 ccggcggtgg caccgaccgc ggcccccgag gcgccgccga tagcgagccc cgcgcccagg
      721 gccaccgcgg ggaagagcgc gacgagcgcg ccgacggcga gcccgaccgc gagaccggcg
      781 atcgctccgt gccgggtggg ctcctcgatc cgctcgacga tcctgacctt cccgtcgggc
      841 cccttggtca cgacggctgc gtcgtacgtg tcgatgatgc cgaggtcctc gtacgcagcc
      901 cgaacggcgt cgtagtcctc gagcgcgtct tcctccaggg cgtactgcga tgcgatcagg
      961 gtgaacgtgt ccaacgccat cgtgcgtgct ccttcccctc gctctcgact cgccgttcct
     1021 tcgtggtgaa cccttacgcc tccataggca tcaccgctcc ggcgacggga gggccgagac
     1081 cgcaagctcg gccagaagca ggcgcgctcg gccgggtgtg tggttggccc gcgcgccaac
     1141 ggaagccact gatccaacgg acagcacgac gcgtgctggc gatgcaggca tacaggtccc
     1201 gagccgattg gtcgctcgcg tctccgtcct acacctggtg gcttcgtggt gcaaggcgag
     1261 gtcgccacgt cgggatcagt tcgcgggatg ttcattgttg aggagcgaag aagttgctcg
     1321 atgcccgctc gctcatttgc ccgggccgcc ctcctcggcc ggtggcggcg agcccgccgc
     1381 ccgctgggcc gcctcgtcga ccctcgtcag ctccgtgtcc tcgacctcgt atcgggtggc
     1441 atacgtcgtc atgaacgcct ggatcgccgc cgccgtgggc agcgcgaaga aggcgccgat
     1501 gaggccaccc accgaccccc ccgccaaggc cgcacccagc gcgatgccgg ggttcagatt
     1561 catcgtctgc tggctcagcc tgggctcgag caggtagttc tcgagctgct ggtagatgac
     1621 gatccaggcc agcaggatga tcgcccgacc tggacccgcc gctgccaggg cgacgacgac
     1681 cggcacgatc ccggccaggt aggtcccgac gatcgggatg aacgccgccg tgaacccggt
     1741 gaagatggcc aacggcaccg cgaacgggat gcctgccagg agcatgacga tgagcatcag
     1801 cgagctgttg atcaccgcca gcaggatgcg ctggtacagg tagccgccgg tcttctcgag
     1861 cgccacgttc catgcccaca ggacgtcccg ctgatgctcc ggtcgcagca gggacacgac
     1921 cgagcggcgg atcctcgggg ccttcgacgt catgtagaac gcgaacaggg cgatcgtgaa
     1981 cacggcgaag atggcgccca cgagaccccc cacggcgccg accagggcac cgccgtagtc
     2041 ggacacgaag ttcttcgcca ggccgacgct cgactgggag gggtggatgg cggtgtcggg
     2101 gatcagttgg acatcgaact cacgctgggt gtacccgttg acgtcgtcga tccaccccgg
     2161 gacgtcgtcc gccatcttcc cgatgttctc gaccatgatg gggatcacca tcagccccag
     2221 gatcaagaag aaggccatga cgccgagcaa cagcgtggcg gtcgccgctc cgcgcgacca
     2281 accacgtcgg tgcaaccgat tcaccgccgg ttcgagcgag agcgagaaga acgcggccaa
     2341 gagcaccagg ccgagcaggt cgtgcgcctg gccgaggaac cagagcaacg cgagggtccc
     2401 gagcacgtac ccgatggtga ggcggagggc gcgcgggaac cacgtgggca tcgaggttga
     2461 aatcgatggt tcggcggctc cggtgggagt gacctcatcg gcgaccgcct tcgggtcggg
     2521 ggtgctcacg cgttcggctc tgcacggacg ccgatgcgag gcctcaccga aaccgtagcc
     2581 tccatggacg gatccacccg agcagagggg gtcaggtgcc gccgaccaat gtaggcgggc
     2641 ccctgcctgg tgtcgaatcg atccgatgag cgctcacccg aatcggccgt agagatcgct
     2701 cgtcggatcg aagaagccgt tcgcgggtcg cgcgtgggcg tgccagtgat cggggatccg
     2761 acggcgttcc gggtcgatcc agaacccatc ggggtagcgt tcggccgcga tccgttcgag
     2821 gtgcgcgagg agccggcgtt cgagctcggg ttcggggaga ccgtgcggtc gccagacgat
     2881 catcggggtt cggcacacga cgcagtcggt gacccagcac tcctcgtcct cgaaatgcca
     2941 gtcggtcacc cgttcggcgc ggcagaggag gcagtcctga ctcatacgcc tgggctcatc
     3001 cgttcgagac cgtcagctcg acccggtcgc cgggggcgac gccgtgccgc tcgaatgtcc
     3061 cccgggcgac ctcgacggcg ccgacgaacg gttcgtcggg gccgtaggag gggcagggct
     3121 cttcggtgca ggggtccatc gcgaggatcg agatgatccg gtcgtggtcg tcccagaacg
     3181 cgatcgagag cgggatgagc gtgtccttca tccagaacgt gctcaccacg ggctccttcc
     3241 atacgaacgc catgccgtcg tacggggaga ggctctcgcg ccccatcagc ccggtctggc
     3301 gttctgcctg cgtgtccgcg acctggacgt cgagcgagac ggtgccgtcg tcggtcccga
     3361 tcgagaggat ggccgacgcg tgcgggtccg gggcctgctc ggccgatccg cacgcgacga
     3421 cgacgagcag cagcaggggg gcgagccgca tccatccatc gtcccatggg tcggggaagg
     3481 gtcggggctc gacggtctct cggtatcatg gcccgcgccg agcaacaggc cgagggaatg
     3541 ggcaaaggaa cacgccgaag gaatacgcca aaggaacacg ccggagtagc tcagtcggca
     3601 gagcacctcc atggtaagga gggggtcagg ggttcgagtc ccctctccgg ctcggaccgc
     3661 cgcgcgacca cgggtcaagc cgcaggtcat ggtggcgagg acggcggcgg ccggtagact
     3721 gctcgtcatc ggggcggggt agctcagagg cagagcaagc ggctcataat cgctgtgtcg
     3781 cgggttcgat tcccgccccc gctacgaacg aacccccgaa cggccagcgg gacgcgccgg
     3841 gacgcagcca ggaacgcaac aggacacgaa acgggtcgga ggaccacgag atggccaagg
     3901 gcgacaacag gccgacgatc accctcgcgt gcacggagtg caagcagagg aactacgtca
     3961 ccgtgaagaa ccgagtgaac gaccgggacc ggatcgagct caggaagtac
//
LOCUS       DATBRK010000473         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2740415, whole genome shotgun sequence.
ACCESSION   DATBRK010000473 DATBRK010000000
VERSION     DATBRK010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740415"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VIH70_13720"
     CDS             complement(<1..589)
                     /locus_tag="VIH70_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8117465.1"
                     /translation="METVTSTDGTTLGFDRLGQGPPVVLVCGGSVDRMADAALAQELS
                     SDFTVLNYDRRGRGDSGDTPPYAIEREVEDIEAVIDAAGGSAHLWGSSSGAVLAMIAA
                     ASGAMVDKLALWEPPFILDENARPPADQVEQYERMIAEGRRGDAAEYFMTKVVGMPPA
                     FVADARKQPWWAGQEAIAHTLAYDARIMGDYSIPTE"
     gene            complement(604..1359)
                     /locus_tag="VIH70_13725"
     CDS             complement(604..1359)
                     /locus_tag="VIH70_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8117466.1"
                     /translation="MAHPFELKHEIEVDASPEEVWDAIATGPGVDSWFMGRNEIQPGE
                     GGSTRMDLRGSTEETTITAWEPPTRFASRTPEGEDGSLHVFEYLVEGRDQGSTVVRWV
                     HSGFLGENWEKEYEGLSEGDPMYFDKLRVYLTYFRGRTATPVNVFGAAVVPDRDEAWK
                     RFHEALGLPGPVVLGDPVHLTPDGLPELDGVVDWLSRDFLGVRTDDGIYRFMHISVFG
                     GPVGVGHHIFVDGLDQAETEAAWEGWLTKVFPS"
     gene            complement(1361..1984)
                     /locus_tag="VIH70_13730"
     CDS             complement(1361..1984)
                     /locus_tag="VIH70_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8117467.1"
                     /translation="MLDLAVIEDPAVAEVSLDPVRSRLLAELAEPASATMLAGKLGLP
                     RQNVNYHLRALEEHGLVELVEERRKGNMTERLLRATALSYVISPAALNGVEPDPSRAP
                     DRLSARWLIAVAAKLVRDVGELMAGATMARRRLATFAMDGEIRFASAADRAAFAEELA
                     AAVTSLVGKYHDEAAPRGRDHRVIVAVHPSLKRDDEGTEPTTDTEGT"
     gene            complement(2063..>2366)
                     /locus_tag="VIH70_13735"
     CDS             complement(2063..>2366)
                     /locus_tag="VIH70_13735"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8117468.1"
                     /translation="MRSDQGWELYNTWTYRSIEPMERIEFVHGFADENGTPVTPAELG
                     LPPGIPDEVRHEVRLSAIDDHTTELTVHEFGYPNAEIVEVSRVGLDQCLDKMATSA"
BASE COUNT          348 a          846 c          774 g          398 t
ORIGIN      
        1 tctcggtcgg gatcgagtag tcgcccatga tgcgggcgtc gtacgcaagc gtgtgggcga
       61 tcgcctcctg gccggcccac cacggctgct tccgcgcgtc ggcgacgaac gccgggggca
      121 tcccgacgac cttcgtcatg aagtactcgg cggcgtcgcc gcgacgccct tccgcgatca
      181 tccgctcgta ctgctcgacc tgatccgccg gcgggcgggc gttttcgtcc aggatgaacg
      241 ggggctccca gagcgccagc ttgtcgacca tggccccact cgccgcggcg atcatcgcga
      301 gcaccgcacc tgacgacgac ccccagaggt gggcggaccc acccgccgcg tcgatcacgg
      361 cctcgatgtc ctcgacctcc cgctcgatcg catacggggg tgtgtctccg ctgtccccgc
      421 ggccgcggcg gtcgtagttc aggacggtga agtcggagga cagttcctgt gcgagggcag
      481 cgtcggccat gcggtcgacc gaaccgccgc acaccagcac caccggtggt ccctggccga
      541 gccgatcgaa cccgagcgtc gtcccgtctg tcgaggtgac ggtttccatg gagacctcct
      601 ctcctaggaa gggaagacct tcgtcagcca gccttcccac gcggcctcgg tctccgcctg
      661 atcgaggccg tcgacgaaga tgtggtggcc gacgccgacc ggaccgccga acacgctgat
      721 gtgcatgaac cggtagatcc cgtcgtctgt gcggaccccg aggaagtccc gggagagcca
      781 gtcgacgaca ccgtccagct ccggcagccc gtccggggtg agatgaacgg ggtcgccgag
      841 gaccaccggt cccgggagac ccagcgcctc gtggaatcgc ttccacgcct cgtcccgatc
      901 gggcaccacc gctgcgccga agacgttcac cggcgtggcc gtccggccgc ggaagtaggt
      961 gaggtagaca cgcagcttgt cgaagtacat cgggtcgccc tcggagaggc cctcgtactc
     1021 cttttcccag ttctcgccga ggaaaccgct gtgcacccag cggacgaccg tgctgccctg
     1081 gtcgcgcccc tcgaccaggt actcgaacac gtggagcgag ccgtcctcac cttccggcgt
     1141 gcggctcgcg aaccgcgtcg gcggctccca tgccgtgatc gtcgtctcct ccgtcgagcc
     1201 acgcaggtcc atgcgggtcg agccgccttc ccctggctgg atctcgttgc ggcccatgaa
     1261 ccaggagtcg acgccgggac cggtggcgat cgcatcccag acctcctccg gcgaggcgtc
     1321 gacctcgatc tcgtgcttga gctcgaacgg gtgtgccatc tcaggtcccc tccgtgtccg
     1381 tcgtcggttc ggttccctcg tcgtcgcgct tcaggctcgg gtgcacggcg acgatcacgc
     1441 ggtgatcccg cccgcggggc gcggcctcgt cgtggtactt gcccaccaag gacgtgacgg
     1501 cagccgccag ctcctccgcg aacgcggcgc ggtccgcggc cgaggcgaac cggatctcgc
     1561 cgtccatcgc gaacgtcgcg aggcgtcttc gcgccatggt cgcgcccgcc atgagctccc
     1621 cgacgtcgcg caccagtttg gccgccacgg cgatcaacca gcgcgccgag agccgatcgg
     1681 gcgcgcgcga gggatcgggc tccacgccgt tcagcgccgc gggagagatc acgtaggaca
     1741 gggcggtcgc tctcagcaat cgttccgtca tgttgccctt tctccgctcc tcgacgagct
     1801 ctacgagccc gtgctcctcg agcgcccgta ggtggtagtt cacgttctgg cgtgggagcc
     1861 cgagcttgcc ggcgagcatc gtcgcggacg cgggctctgc gagctcggcc aggagccgcg
     1921 atcggacggg gtcgagggat acctcggcca cggccgggtc ctcgatcacc gcgagatcca
     1981 acatgccggt catgttccca ccgaaactct ttcctgtcaa ggaaaagaga accgtcggtg
     2041 gatctcggga cgggttaggc ccctaggcgc tcgtcgccat cttgtcgaga cactggtcca
     2101 ggccgaccct cgagacctcg acgatctcgg cgttcgggta cccgaactcg tggaccgtca
     2161 gctcggtcgt gtggtcgtcg atcgccgaca ggcgtacctc gtggcgcacc tcgtccggga
     2221 tccccggggg aaggcccagt tcggccggcg tgaccggggt gccgttctcg tcggcgaatc
     2281 cgtggacgaa ctcgatccgt tccatcggct cgatcgatcg atacgtccag gtgttgtaga
     2341 gctcccaccc ctggtccgac cgcatg
//
LOCUS       DATBRK010000474         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_561227, whole genome shotgun sequence.
ACCESSION   DATBRK010000474 DATBRK010000000
VERSION     DATBRK010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561227"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VIH70_13740"
     CDS             <1..216
                     /locus_tag="VIH70_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8117469.1"
                     /translation="RDVLRALASDFDAAHHPVADWMTHDPTTVGIEADVRTARDLMLD
                     GGFRHLPVLDGAELVGIVSIRDISRAD"
     gene            complement(247..435)
                     /locus_tag="VIH70_13745"
     CDS             complement(247..435)
                     /locus_tag="VIH70_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117470.1"
                     /translation="MARELDWAVFEKAVEITASAVRGAMGGEKSQPASYAGDVFKDVW
                     GALKAAVEELPERGRTGF"
     gene            complement(486..1655)
                     /locus_tag="VIH70_13750"
     CDS             complement(486..1655)
                     /locus_tag="VIH70_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8117471.1"
                     /translation="MAESPDPLDFLDLDHLLADEERAVRDTVRDWVRDRVLPGIETWF
                     EAGEFPLEVAKELGAMGLLGMHLTGYECAGSNAVSYGLACLELEAGDSGFRSFVSVQG
                     SLCMFPIWRFGSEEQKQRWLPPMARGEVIGCFGLTEPDVGSDPGRMRTSARRDGDDWV
                     LSGTKMWITNGSVADLAIVWAGTDDGIRGFLVPTGTPGFSANDVHRKLSLRASVTSEL
                     VLDEVRLPADAVLPGVTGLKGPLSCLSEARFGIVWGSMGAARACFESALAYATTRVQW
                     GKPIGGFQLTQRKLADMAVELNKGTLLAVHLGRMKDEGRLAPQHVSMGKLNNVREALE
                     IAREARTILGASGITLEYPVIRHMNNLESVLTYEGTTEIHTLILGEALTGLRAYE"
BASE COUNT          262 a          655 c          572 g          260 t
ORIGIN      
        1 cgggacgtgc tccgggcgct cgcgagcgac ttcgacgccg cccatcaccc ggtcgcggac
       61 tggatgacgc acgaccccac caccgtcggc atcgaggccg acgtcaggac cgcgcgagac
      121 ctgatgctgg acggcggctt caggcacctg ccggtgctgg acggcgccga gctcgtcggg
      181 atcgtctcca tccgggacat ctcgcgcgcc gactgacgag ccccctggaa gctggctgga
      241 agccggctag aagccggtac gacctcgctc cgggagctcc tcgacggccg ccttgagcgc
      301 gccccatacg tccttgaaca cgtcgccggc gtaggacgcc ggctggctct tctcgccccc
      361 catcgcgcct cgaacggcgg atgcggtgat ctccacggcc ttctcgaaca ctgcccaatc
      421 gagctctcgc gccatcgcgg atcggacctc cggtgtcggt ttcggacagg ctacccgccg
      481 cggcgctact cgtacgcgcg gagcccggtc agcgcctcgc cgaggatcag cgtgtggatc
      541 tcggtcgtac cctcgtaggt cagcacggac tcgaggttgt tcatgtgccg gatcaccggg
      601 tactcgagcg tgatcccgct cgcaccaagg atcgtgcggg cctcgcgggc gatctcgagc
      661 gcctcgcgga cgttgttgag cttgcccatg ctcacgtgct gcggcgcgag ccgcccctcg
      721 tccttcatgc gccccaggtg caccgcgagc agcgtcccct tgttgagctc gaccgccatg
      781 tccgcgagct tgcgctgggt cagctggaac ccgccgatcg gcttgcccca ctggacccgc
      841 gtggtcgcat acgcgagcgc cgactcgaag caggcgcgcg ccgcgcccat cgaaccccac
      901 acgatgccga accgcgcctc ggacaggcag gagagcggcc ccttcagtcc ggtgacgccc
      961 gggagcaccg cgtcggcggg cagccgcacc tcgtccagga cgagctccga ggtcacgctc
     1021 gcgcgcagcg agagcttgcg atgcacgtcg ttcgccgaga agccgggcgt cccggtcggg
     1081 acgaggaacc cgcggatccc gtcgtcggtg ccggcccaga cgatcgcgag gtcggcgacg
     1141 ctcccgttgg tgatccacat cttggtcccc gacaggaccc agtcgtcccc gtcgcgccgg
     1201 gcgctcgtgc gcatgcggcc ggggtccgag cccacgtcgg gttcggtcag gccgaagcac
     1261 ccgatcacct cgccgcgggc catcgggggc agccaacgtt gcttctgctc ctcggagccg
     1321 aacctccaga tcgggaacat gcagagcgac ccctgcacgc tcacgaacga ccggaacccg
     1381 ctgtccccgg cctcgagctc cagacacgcg agcccgtagc tcaccgcgtt cgaacccgcg
     1441 cattcgtagc ccgtgaggtg catcccgagc agccccatcg cgccgagctc cttggcgacc
     1501 tcgagcggga actcccccgc ctcgaaccag gtctcgatgc ccgggaggac gcggtcgcgc
     1561 acccagtctc ggaccgtgtc gcggaccgca cgctcctcgt cggcgagaag gtgatcgagg
     1621 tccaagaaat ccaggggatc cggtgattcg gccatggcgc ttcgctagcc tagcgtcgtc
     1681 ccgtacccca tggcccgagg cacggtctga gcgaggggcg gccgcgcggc cgccataacc
     1741 gacgatgct
//
LOCUS       DATBRK010000475         6950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1587695, whole genome shotgun sequence.
ACCESSION   DATBRK010000475 DATBRK010000000
VERSION     DATBRK010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587695"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /gene="pstB"
                     /locus_tag="VIH70_13755"
     CDS             complement(<1..773)
                     /gene="pstB"
                     /locus_tag="VIH70_13755"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY8117472.1"
                     /translation="METTDLTHLPEPDDPKAPPEQGGPVSVRIEREPRHLEEAEQEVV
                     FDVRDLSVFYGDFRAVRDVTLPIYKHQITALIGPSGCGKTTFIRCLNRMNDLIEGARV
                     EGTLLYHGVDLYDEQVDPVEVRRRIGMVFQKPNPFPKSIYENIAFGPKIAGFKGDMDE
                     LVEESLRRAALWDEVKDKLKESGLALSGGQQQRLCIARAIATRPDVILMDEPCSALDP
                     IATQRIEDLMQELLSDYTIVIVTHNMQQAARVSDRTAFFT"
     gene            complement(778..1722)
                     /gene="pstA"
                     /locus_tag="VIH70_13760"
     CDS             complement(778..1722)
                     /gene="pstA"
                     /locus_tag="VIH70_13760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY8117473.1"
                     /translation="MSLVAEADTRTAVNLALTGRRRHLGGMLFRFGLLATLLLALAIL
                     LVLLVSTLIEAWPVLSTRGWGFVTNDSSSLSERAGVWQGLVGSLILVGFVAAIALPIG
                     VAAAIYLQEYTRDTRTSRILIANIRNLAGVPAVVYGILGLVVFVKALSAITGPDSHGR
                     SYISGGLTLAVLVMPIVILITMEALRAVPPSIREGAYGVGATRWEVVRSHVLPNAAPG
                     IFTGAILAMARAFGETAPLLLVGAVTGYFSSASGRTPLEVLQGPYTALPTQIFSFAKL
                     PANEWQPNTAAAIIVLLVAILLVNFLAILLRNRYERTW"
     gene            complement(1722..2663)
                     /gene="pstC"
                     /locus_tag="VIH70_13765"
     CDS             complement(1722..2663)
                     /gene="pstC"
                     /locus_tag="VIH70_13765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY8117474.1"
                     /translation="MSATATTRQPITLEVMRGSKRRHRKERAVRTFFQAAAFLGLVIS
                     IAIVLALIGEAIRWLFDIQLGWLSTGGWIPRSNQFDLLTIFAGTVMVAVIAMLVAAPL
                     GLGAAAYLSEYATPRTRRTLKPILETLASIPSVVLGYFALQVITPDLVRKIFASAGTF
                     NYLAAGLAVGILTIPLVASVAEDAMHAVPNALREGAYGIGARRMTVTLRVVFPAAVSG
                     IVASLILGFSRAVGETMVVAIAAGATGSAERTLNPLDGGQTMTGAIASLAIGSDQVRG
                     SGFAFDSLYFVGLLLFALTFLLNVVSERFVRRVRSRY"
     gene            complement(2751..3824)
                     /locus_tag="VIH70_13770"
     CDS             complement(2751..3824)
                     /locus_tag="VIH70_13770"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8117475.1"
                     /translation="MGKRRNGLLVLAMIAVLALVAAACGDDDGGGETGSTTGATGATA
                     LSGTITISGSSTVQPITSLVAELFNEDVSSDVSITVDGPGTGDGFVLFCDGETDIQDA
                     SRPIDPKEEAPVCAKNGIEYVELAIAFDGITVMTNPTNGDVTCLNKGDLYSLFGPESD
                     GIDTWDAADSLAQQVGGTDNFPSAPLEITAPGTESGTYDAFIELAGIEDTALSQGVPE
                     DKAAALRTDYHSSPDDNAIITAMEGSDTPLGFVGFAFAENAGDQVKILQVDGGDGCIE
                     PTRDTIADQSYPLSRTLYIYVDKAKISDPAIKAFVDYYLSDSGIVDAVQQTGYVDLPS
                     DQIESSRSTWTSASGGTGATGSS"
     gene            3981..5126
                     /locus_tag="VIH70_13775"
     CDS             3981..5126
                     /locus_tag="VIH70_13775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8117476.1"
                     /translation="MDAVPLLVALGGILFGVASLALLRRERVRVRTQARERARREDEF
                     QRERAAADDARRSADLVLSSMEDGVLLFGPDGRTRLSNPAVEAQLGVRPASMEALLPL
                     GLRHLAERAATTQAVLAVEVETGSPSRTLRGFAAPAREGSVVLVIRDITETRRLDRVR
                     RDFVANASHELKTPAASIQAAAETIRTAAAEDPSVIPRFASQLEREAARLSRIVSDLL
                     DLSRLESGSVLDEAVALDAIVRDEGERFEDPAAEAGVGLSVAAAAVPRVRGSARDLAL
                     LVRNLVDNAIRYTPPGGKVHVELTADGADVVLAVADTGLGIPHRDLPRIFERFYRVDR
                     ARSRETGGTGLGLSIVKHVAENLGGRVEVTSELGQGTRFEVRLPASP"
     gene            5173..5805
                     /locus_tag="VIH70_13780"
     CDS             5173..5805
                     /locus_tag="VIH70_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HEY8117477.1"
                     /translation="MATLLLVRHGHTDAAGKRLTGWAPGVHLNETGRRQAERLVERLD
                     GIRIDAIYSSPLERCRETAAPLAKARRRSVHVRRRLIETGYGEWTGRSIAQVRKTKLW
                     RTVERVPSAMRFPGGESLLEVQARAVDEVNRIARDHPRGVVVVVSHADPIRLVIAQVA
                     GADVDHLHRLVVDTGSITAVSLDDAVPRLLKVNDTGDLAGLRSRPRKVGG"
     gene            5810..6391
                     /locus_tag="VIH70_13785"
     CDS             5810..6391
                     /locus_tag="VIH70_13785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEY8117478.1"
                     /translation="MDLERVDRITAGAVGEPGERTFFLQAREGDRSITILVEKEQVEL
                     LGTSILEILATVGRETGEGPPSDELGLEPPLEPLWRAGRLSIGYAEERDLMLLEIEEL
                     VELEDGPEDEPDVEEDEADDEPVGAPLTETDGPEPDRVRLWATREQMLALARHGAAVA
                     ERGRPRCRYCGNPIDPDGHMCPAMNGHRESAGR"
     gene            6388..>6950
                     /locus_tag="VIH70_13790"
     CDS             6388..>6950
                     /locus_tag="VIH70_13790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol kinase"
                     /protein_id="HEY8117479.1"
                     /translation="MTFGEPERQPSERRTDGPDGPDGNDVERIRGDAAALALLERGTI
                     EVLGLLPRASNFTFLARVRADRDQMLAVYKPRSGEAPLWDFEGGTLAAREVAAYVVAD
                     ALGWPWVPPTVLREGPQGTGSVQRFVAFDPDQHYLTMQHDRADEFRKIALFDVVANNA
                     DRKSGHCLLAEDGRIFVVDHGVCFHVDP"
BASE COUNT         1048 a         2397 c         2489 g         1016 t
ORIGIN      
        1 gtgaagaacg cggtgcggtc cgagacgcgg gctgcctgct gcatgttgtg cgtcacgatc
       61 acgatcgtgt agtccgacag gagctcctgc atcaggtcct cgatccgctg cgtggcgatc
      121 gggtcgagcg ccgagcaggg ctcgtccatg aggatcacgt ccggtcgcgt cgcgatcgcc
      181 cgggcgatgc acaggcgctg ctgctgtccg ccggacagcg ccaggcccga ctccttgagc
      241 ttgtccttga cctcgtccca cagggccgcc cggcgcagcg actcctcgac gagctcgtcc
      301 atgtcgccct tgaagccggc gatcttgggc ccgaaggcga tgttctcgta gatcgacttg
      361 gggaacgggt tcggcttctg gaacaccatg ccgatgcgcc gacggacctc gacgggatcg
      421 acctgctcgt cgtacagatc cacgccgtgg tacaggaggg tcccctcgac gcgcgctccc
      481 tcgatcagat cgttcatccg gttgaggcag cggatgaagg tggtcttgcc gcacccggac
      541 gggccgatga gcgccgtgat ctggtgcttg tagatcggga gggtcacgtc gcgcaccgca
      601 cggaagtctc cgtagaagac cgagaggtcg cggacgtcga agacgacctc ctgctcggcc
      661 tcctcgaggt ggcgcggctc gcgctcgatg cgcaccgaga ccgggccgcc ctgctcgggg
      721 ggggccttcg ggtcatcggg ttcgggtagg tgggtcaggt ccgtggtctc catgtgatca
      781 ccaggttcgc tcatatcggt tcctcaacag gatggcgagg aagttcacca ggaggatcgc
      841 caccaacagc acgatgatgg cggctgccgt gttcggctgc cactcgttgg ccggcagctt
      901 ggcgaacgag aagatctgcg tgggaagcgc agtgtacggg ccctgcagga cctccagcgg
      961 ggtgcggccc gaggccgacg agaagtatcc ggtgaccgcg cccaccagca gcagcggcgc
     1021 ggtctctccg aacgcgcggg ccatcgcgag gatcgcgccc gtgaagatgc ccggcgcggc
     1081 gttcgggagc acgtgcgacc gcacgacctc ccagcgcgtg gctcccacgc cgtacgcgcc
     1141 ctcgcggatg ctgggcggca ccgcgcgcag cgcctccatc gtgatcagga tcacgatcgg
     1201 catgaccagg acggccagcg tgaggccgcc cgagatgtag ctgcgcccgt ggctgtcggg
     1261 gccggtgatc gccgacaggg ccttcacgaa gaccacgagt ccgaggatgc cgtacacgac
     1321 cgcgggaacg ccggcgaggt tgcggatgtt cgcgatcagg atccggctgg tccgcgtgtc
     1381 tcgtgtgtac tcctggaggt agatcgcggc ggcgaccccg atcggcaggg cgatcgctgc
     1441 gacgaacccg accaggatga gcgacccgac cagcccctgc cagacccccg cgcgctcgga
     1501 caggctcgac gagtcgttgg tgacgaagcc ccatcccctg gtcgaaagga ccggccaggc
     1561 ctcgatcagc gtgctcacca acaggaccag caggatggcc agcgccagca gcagggtcgc
     1621 gagcaggccg aagcggaaca gcatccctcc gaggtgccgc ctgcggccgg tcagcgcgag
     1681 gttgacggcg gtcctggtgt cggcctcggc gacgagcgac atcagtacct gctcctcacc
     1741 cggcgcacga accgctcgga gaccacgttg agcaggaacg tgagggcgaa caacagcagc
     1801 ccgacgaagt acagggaatc gaacgcgaag cccgagccac ggacctgatc cgatccgatc
     1861 gcgagcgagg cgatcgcgcc ggtcatcgtc tgcccgccgt ccaaggggtt gagggttcgt
     1921 tccgcgcttc cggtcgcgcc ggccgcgatc gcgaccacca tcgtctcccc gacggccctc
     1981 gagaagccga ggatcagcga ggccacgatg ccggagaccg cggcggggaa cacgacccgt
     2041 agggtgaccg tcatgcgccg ggctccgatc ccgtacgcac cctcgcgaag cgcgttgggg
     2101 acggcgtgca tcgcgtcctc ggccaccgag gccaccagcg ggatcgtgag gatcccgacg
     2161 gccaggccgg ccgccaggta gttgaaggtg ccggccgacg cgaagatctt gcgcacgagg
     2221 tccggagtga tcacctgcag cgcgaagtac cccaggacga cgctcgggat gcttgccagt
     2281 gtctccagga tcggcttgag ggtccgccgt gtcctcgggg tcgcgtactc ggacaggtag
     2341 gcggccgctc cgaggcccag cggcgccgcc acgagcatgg cgatgaccgc gaccatcacc
     2401 gtccccgcga agatcgtgag caggtcgaac tggttggacc gcgggatcca cccaccggtc
     2461 gacagccaac cgagctggat gtcgaacagc caccggatcg cctccccgat gagcgccagc
     2521 acgatcgcga tgctgatcac caggcccagg aaggctgcgg cctggaagaa ggttcggacc
     2581 gcccgctcct tgcggtgccg tcgcttgctt ccccgcatga cctcgagggt gatcggctgc
     2641 cgggtggtgg cggtcgcgct catgggtcgg tcgagcctac cctcctcgtg cgagggtcgc
     2701 ggacgggacc gggcgcgaag cgcgccccgg tcccgtcccg acgtgatcgg ctacgagctg
     2761 ccggtcgctc cggtaccacc cgacgcggag gtccacgtcg accgactcga ctcgatctgg
     2821 tcggacggca gatcgacgta gcccgtctgc tggacggcgt cgacgatgcc gctgtcgctc
     2881 aggtagtagt cgacgaaggc cttgatggcc gggtcggaga tcttcgcctt gtccacgtag
     2941 atgtacagcg tgcgggacag cgggtaggac tggtcggcga tcgtgtcgcg cgtcggctcg
     3001 atgcatccgt cgccgccgtc gacctgcagg atcttgacct ggtctccggc attctccgcg
     3061 aaggcgaacc cgacgaaccc gagcggggtg tcggagccct ccatcgccgt gatgatggcg
     3121 ttgtcgtccg gcgaggagtg gtaatccgtc cgcagggccg ccgccttgtc ctccggcacg
     3181 ccctgcgaga gcgccgtgtc ctcgatgccg gcgagctcga tgaacgcatc gtaggtcccg
     3241 gactccgtcc cgggcgcggt gatctccagc ggtgcgctcg ggaagttgtc ggtgccgccg
     3301 acctgctggg cgagggagtc cgcagcgtcc cacgtatcga taccgtcgga ctccgggccg
     3361 aacagggagt acaggtcgcc cttgttcagg cacgtgacgt cgccgttggt cgggttggtc
     3421 atgacggtga tgccgtcgaa cgcgatcgcg agctcgacgt actcgatgcc gttcttcgcg
     3481 cagaccggcg cttcctcctt cgggtcgatc ggccgcgagg cgtcctggat gtcggtctcg
     3541 ccgtcgcaga acaggacgaa tccgtcaccc gtccctgggc cgtccaccgt gatcgagacg
     3601 tcggaggaca cgtcctcgtt gaagagctcg gcgaccaggc tcgtgatcgg ctggacggtc
     3661 gaggagcccg agatcgtgat ggtgccgctc agagcggtcg ctccggtcgc tccggtggtc
     3721 gagcctgtct cgccaccgcc gtcgtcgtca ccgcaggccg ccgcgacgag cgccagcacg
     3781 gcgatcatcg cgagcacgag caggccgttc ctgcgcttcc ccatccgaac ctccttgtcg
     3841 agcgggccgg gtcgtcgcgc ggacgctatc cagtggcggt gaacggccac aggctcggac
     3901 gtgaacaccg ggtgaacgac gccctgcgtg tgaacaaatc gtgacggcgc ggccgccgcc
     3961 actccctaca ctgcccgacg atggacgccg tcccgctcct cgtcgcgctc ggcgggatcc
     4021 tgttcggcgt cgcctcgctg gcgctcctgc gcagggagcg ggtacgcgtt cgaacacagg
     4081 ctcgggagcg cgcccggcgc gaggacgagt tccaacgcga acgcgcggcc gccgacgacg
     4141 cccgtcggtc ggccgacctc gtactgtcct cgatggagga tggcgtgctg ctgttcgggc
     4201 ccgacgggag gaccaggctc tccaacccgg ccgtcgaggc gcagctgggc gttcggccgg
     4261 cctccatgga ggcgctgctc cccctcggcc tgcggcacct cgccgaacgg gcagccacga
     4321 cccaggcggt gctcgcggtc gaggtcgaga ccggctcgcc gtcccgaacg ctccgcggct
     4381 tcgccgcccc cgcgcgggag ggctcggtcg tgctcgtgat ccgcgacatc accgagaccc
     4441 ggcggctgga ccgggtgcgc cgagacttcg tggccaacgc gtcgcacgag ctgaagacgc
     4501 ccgccgcctc gatccaggca gcggccgaga cgatccggac ggcggccgcc gaggacccct
     4561 cggtgatccc gcgcttcgcc tcccagctcg agcgcgaggc cgcgcgcctg tcgcgcatcg
     4621 tgtcggacct gctcgacctg tccaggctcg agtcggggag cgtgctcgac gaggcggtgg
     4681 cgctcgacgc gatcgtccgg gacgagggag agcggttcga ggacccggcg gccgaggccg
     4741 gcgtcgggct ctcggtcgcg gcggcggccg tgccgcgggt tcgcggctcg gctcgtgacc
     4801 tcgccctgct ggtgcgcaac ctggtggaca acgcgatccg gtacaccccg cccggcggca
     4861 aggtgcacgt cgagctcacg gccgacggcg ccgatgtggt cctcgcggtc gccgacaccg
     4921 gactcggcat cccccaccga gacctgcctc ggatcttcga gcgcttctat cgagtggatc
     4981 gcgcccggtc gcgcgagacc ggcggcacgg gcctgggtct gtcgatcgtg aagcacgtcg
     5041 ccgagaacct cggcggtcgc gtcgaggtga cgagcgagct cggacagggc acccgcttcg
     5101 aggtccgtct gccggcgtcg ccctgaccgt tgcagggcgg tctcgcgcga gggcgcgggt
     5161 acgctacgcc gcgtggcgac gttgctcctg gttcgacacg gtcacaccga cgccgccggc
     5221 aagcggctga cgggctgggc acccggcgtg cacctgaacg agaccggccg gcggcaggcc
     5281 gagcgcctgg tcgagcggct cgacgggatc cgcatcgacg cgatctactc cagccccctc
     5341 gagcgctgcc gcgagaccgc cgcgccgctc gcgaaggccc gccggcggag cgtgcacgtc
     5401 cgtcgccgcc tcatcgagac ggggtacggc gagtggacgg ggcggtcgat cgcccaggtc
     5461 cggaagacca agctgtggcg aaccgtggaa cgggttccct cggcgatgcg gttccccggg
     5521 ggcgagagcc tgctcgaggt gcaggcgcgt gcggtcgacg aggtcaaccg gatcgcccgg
     5581 gaccaccccc ggggggtcgt cgtggtggtg tcgcacgccg atccgatccg cctggtgatc
     5641 gcccaggtgg ccggcgccga cgtggaccac ctccatcgcc tcgtcgtcga caccggttcg
     5701 atcacggccg tctcgctcga tgacgcggtg ccccgactcc tgaaggtgaa cgacacgggc
     5761 gacctcgccg ggctccggtc gcgcccgagg aaggtgggag gataggtcgg tggatctcga
     5821 acgcgtcgac cgcatcacgg cgggcgccgt cggcgagccg ggcgagcgca ccttcttcct
     5881 ccaggcgcgc gagggcgacc gatcgatcac gatcctggtc gagaaggagc aggtcgagct
     5941 cctgggaacg tcgatcctgg agatcctcgc gacggtcgga cgcgagaccg gcgaggggcc
     6001 tccgagcgac gagctcggcc tggagccgcc gctcgaaccg ctttggcgcg cgggtcgcct
     6061 ctcgatcggc tacgccgagg agcgggacct gatgctgctc gagatcgagg agctcgtcga
     6121 gctagaagac gggccagagg acgagccaga cgtcgaagag gacgaggccg acgacgagcc
     6181 ggtgggcgcg cccctcaccg agacggacgg ccccgagccc gatcgagtcc gcctgtgggc
     6241 gacccgcgag cagatgctgg cgctcgcgcg ccacggcgcg gccgtcgcgg agcgcgggcg
     6301 gccgaggtgc cggtactgcg ggaacccgat cgatccggat ggccacatgt gccccgcgat
     6361 gaacggacac cgcgagagcg cgggccggtg accttcggcg agcccgagcg gcagccgtcg
     6421 gagcgccgga ccgacggccc cgacggcccc gacggcaacg acgttgaacg gatccgaggc
     6481 gacgccgcgg cgctcgcgct cctggagcga ggcacgatcg aggtcctcgg cctgctcccc
     6541 cgcgcctcga acttcacctt cctcgcccgg gtgcgcgccg atcgggatca gatgctcgcc
     6601 gtctacaagc cgcgctcggg cgaggcgccg ctctgggact tcgagggggg aacgctggca
     6661 gcccgggagg tcgcggcgta cgtggtggcg gacgcgctcg gctggccgtg ggtgccgccg
     6721 accgtcctgc gcgaggggcc tcagggaacg ggttcggtgc aacggttcgt ggcgttcgac
     6781 ccggaccagc actacctcac gatgcagcac gaccgcgccg acgagttccg caagatcgcg
     6841 ctcttcgacg tggtcgcgaa caacgccgat cgcaagtccg gccactgcct gctcgccgag
     6901 gacgggagga tcttcgtggt cgaccacggc gtctgcttcc acgtcgaccc
//
LOCUS       DATBRK010000476        10139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_563811, whole genome shotgun sequence.
ACCESSION   DATBRK010000476 DATBRK010000000
VERSION     DATBRK010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563811"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VIH70_13795"
     CDS             <1..205
                     /locus_tag="VIH70_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117480.1"
                     /translation="GEQRDLLRDLTAPSIIRADTIRQFRERPDGQRMADVLMDFEADE
                     TLRLHVVEALRRSLASPGGQPEQ"
     gene            complement(1941..2267)
                     /locus_tag="VIH70_13800"
     CDS             complement(1941..2267)
                     /locus_tag="VIH70_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117481.1"
                     /translation="MMQISETRIVSPDPRQALNAVRSLRRTLARLGLPDRVSSDAGGE
                     LEEIAAELRMPDPDRGLVIARLERFTEILAGAGVDMTLRSLIRSIRQIAAWLGPIGVP
                     LVVRLA"
     gene            complement(2416..3153)
                     /locus_tag="VIH70_13805"
     CDS             complement(2416..3153)
                     /locus_tag="VIH70_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY8117482.1"
                     /translation="MEVLGLRAPRAARGSIVFAAVVAITVVGALSASAAPTVDERELL
                     GESYQGRPIRVFHRGDPDEIRVLVVGCIHGDECAGVRIAKRLRTGRPRSFLDLWILPS
                     LNPDGRAARTRQNARGVDLNRNFPYDWRSGPRGRYYPGPRPASERETRIAMRLIRRIE
                     PDITIWFHQPLALVDASGDVTVERRYARLVGLPLVHLGPLHGTATSWQNHILGRTDAF
                     VVELPGGVVRPRRARRFASAVYRLVRA"
     gene            complement(3135..3344)
                     /locus_tag="VIH70_13810"
     CDS             complement(3135..3344)
                     /locus_tag="VIH70_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117483.1"
                     /translation="MSMEGPGETESRDPGAQRWGAEKEEIDKQAREEHLAHEAERIHA
                     EEAAEMAAERGEAPRPKRPWWKFWG"
     gene            complement(3435..4586)
                     /locus_tag="VIH70_13815"
     CDS             complement(3435..4586)
                     /locus_tag="VIH70_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117484.1"
                     /translation="MRPMRAAICVLSAGMLMMLAACTGASTQVASTSGSGTGSSSSAV
                     ITRFANGRPLPAGCPPGDNARAQTVAFVAGGNAWVLDPESNDASCLFPTPAPGPFAWN
                     PRGDRMLLDGLSIRSIDGKHLRDATSPTSPLSSWGHPIGKAVVYIPPSGTSLLKVYPG
                     TQRTDDITPVPGVRYLNVIYHPSGLALAFVVDTGDGQEIWLSSNVGEDPVRLVFGVGE
                     TTFGALAFTADGRTLLYAAVHGDDAPLLHALDLTDPTINQGLWHGDAGDRISEIFTQP
                     KRRGGLVALTVGSSCEDSRAMVFRRGEQPRTLADAPSRVVGWLDASTVLVASDGCEGA
                     SNLTAVDVIRDAMIPLVDGVDIAATRTPLLGYVPPLPQHIEEEVGSGVG"
     gene            4647..5576
                     /locus_tag="VIH70_13820"
     CDS             4647..5576
                     /locus_tag="VIH70_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY8117485.1"
                     /translation="MSDSFDLLVLGDANPDLVLTGDVEPAFGQEERLVDAARLTVGGS
                     GAIVAAGAARLGLRVGFCGVVGDDAFGRFMLDELERRGVDVGGLVVDSARPTGVTVVL
                     ARPNDRAILTHAGTIADLRTELIDPARLELARHVHVSSYFLQRSLAPELPAVFGRVRA
                     AGATTSVDPNWDPSGRWDGGLRDLLGQVDVFFPNATEATRIAGIDELDDAVLALAECA
                     GVVVAKAGAAGALAAHGERLVRAAAPAIETLDATGAGDAFDAGYLASMLAGDPLERSL
                     AIANACGALSTRALGGVDAQPTMEEVLAGLGED"
     gene            complement(5579..6703)
                     /gene="ugpC"
                     /locus_tag="VIH70_13825"
     CDS             complement(5579..6703)
                     /gene="ugpC"
                     /locus_tag="VIH70_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8117486.1"
                     /translation="MARVAFDGVTKVFPGPVVAVDDLTLEVADGEFLILVGPSGCGKS
                     TALRMVAGLERISDGTIAIGDRVVNDVPPKDRDIAMVFQNYALYPHMTVERNLGFGLR
                     RRRMPREDVRRRLDEVSTMLGLENLLGRRPGQLSGGQRQRVAMGRALVREPEVFLLDE
                     PLSNLDAKLRVQMRAELKRLHERIGVTTIYVTHDQVEAITLGERIAVLSEGVLQQVGP
                     PQEVYDHPANVFVAGFIGSPPMNLLKGTARRGRVVVGEVELEQARAPDGDVLVGIRPE
                     GLRPVGEGHAGPVFDVRVEVVEPLGDEVLVHGSVAALDGSVPADDEAILVNETRDGRA
                     SVAVRLPPEERPSTGSRLRVAIAPNAVRLFDPASGRAISS"
     gene            complement(6708..7619)
                     /locus_tag="VIH70_13830"
     CDS             complement(6708..7619)
                     /locus_tag="VIH70_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8117487.1"
                     /translation="MSAVTEAPAVPTRADPRSLPGETGMVERPRRRFLPFDPWHLFLA
                     PLAAVMLLPLLWMVVTSLETEAQTFHFPPILWPGTLRFGNYAEVLGESAFGTWFWNTT
                     VVTVTVVASNLVLCTLAAYAFARIRFLGRTFVYFVILATLMVPLQVVLIPTFLIVKDL
                     GLLDRLGALVVPNLVNVFGIFLLTQFFRTLPIELEEAARIDGASRLSILLRIVVPLSL
                     PALATLAVIQFLWTWNDFLWPLVTILTNQDAFTLQLGLATFQGAHNTQWHLVSAANIM
                     SMVPMIVLFLLAQRYFVRGIATQGLKG"
     gene            complement(7616..8524)
                     /locus_tag="VIH70_13835"
     CDS             complement(7616..8524)
                     /locus_tag="VIH70_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8117488.1"
                     /translation="MDRFRRFLVSDHAAGWAFILPSVILIAVFGIIPILWGLILSFQK
                     AGLISPEREWVGLSNYAKIASDPKARQAAANTIYYTALFVPASILIALFIAHALNRRI
                     AFVRFYRLAVFIPVVTSTIATGIMFLWLFDKNYGLANWLLGKVGLGPFGFFEDPNQAM
                     IALVLMTVWGWLGFDTIIYLAALQGVPQELLEAASIDGASRWSSFWRVTWPMLGPATL
                     LLVVWSTISALQLFDEVFFVTHGGPLGRTSVVVFYVYRLAFEQGLGSLSLAGYAAAIA
                     YVLFLVILLLTLAQFWLGRRVVHYSS"
     gene            complement(8560..9849)
                     /locus_tag="VIH70_13840"
     CDS             complement(8560..9849)
                     /locus_tag="VIH70_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8117489.1"
                     /translation="MRRRMLVVLVVLGLLAAACTGGDDKSATTGETASGPVTLTFWHG
                     YTDYEADSLNALLDQWNAENPDITIDPLFVNNDKALQKLTVALQGGEPPDITYQYGSS
                     LPQIAEAPGLVDLTDWVQSPEVDWEDFVAGARDAATFEGRVLGVPALIDNLALVYNKT
                     LFDDAGLEYPNADWTWDDFRAAAKALTDPAKKQFGFSYPMDASEDSVWHYDPLLWQNG
                     GSILTEDNTQAAFNSPEGVEALEVLTGMAVQDKSVFLDLQNSPYTGLFNSGKIGMLVT
                     GPWDLGSFPDVDYGVEILPGFDGDHQTIAGPDMWTVFDNGDGRAQAALDFVAWLTAPE
                     QMKADALAAGHLPFRLSVIDDTAFIDEFGKTIPGVDVFAENLTNVEQARPVLAAYPQV
                     SEAMGLAIVSAMLGEKDPQTALDDAAAQANDALALAG"
     gene            complement(9884..>10139)
                     /locus_tag="VIH70_13845"
     CDS             complement(9884..>10139)
                     /locus_tag="VIH70_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HEY8117490.1"
                     /translation="AYPAMEYRHGPISLAGERSLVWILGSPDPAVAEDVRATGATVRV
                     ASLDPMAELILVHRMAIALAEAKDLDPDHPAHLTRSVVLP"
BASE COUNT         1620 a         3488 c         3458 g         1573 t
ORIGIN      
        1 cggcgagcaa cgggatctac ttcgggattt gaccgctccc tcgatcatcc gagcggacac
       61 gatccgacag ttccgcgaac ggccggacgg acaaagaatg gccgacgtcc tgatggattt
      121 cgaggctgac gagactcttc ggcttcatgt ggtggaagca cttcgtcggt cacttgcttc
      181 gcctggaggc caaccggagc aataggcacg cccggcctgt cccacttttc gccccggaag
      241 atccaagact gcggtactga cggctacttc gaccagcaag atcatgagcc gaatcgcatg
      301 gggccatcgt cagttcggcc tccgcgttga ccgtcctccc cgtcgtcatt tcccatccct
      361 gacgtagtct ctgagtgatg gaacgtcacg ccgttctcta cgacgaggac tgcggttttt
      421 gccgatggtc gctcgacaag attctccaat gggatcgggg ccgtcaggtt cgggcagtcg
      481 cgatccagag cgacgaggga gaccggctac tcgccgactt gagcgaggac gcgcggctcg
      541 cttcgtggca tttcgtcact tccgatgggc gacgcttctc gggtggttca ggggcaggtc
      601 cgctcgctcg cctccttccc ttcggacgtc ctatcgctgt tctggccgag accttccctc
      661 gcacgaccga ccctgtctac cggtgggtgg cgcggcaccg agactggctc ggtcgacggg
      721 tcggctcgca cgcctgcgca gtcgaccctc gtcgacctac tcggcgcgca cgttccaaca
      781 tggcatagcg acgaacttga acgaaccgtg aaaggaagtg tcgctggcat acctcgcatt
      841 gccgttccgg gtcggggcta tcgcgtcacg cggtagcgga ggtaaacgac gctcgagctg
      901 aaggtgcggg tctcgacgag ttcgacggct tcgagctaga tcgggcggtc tgagctccgg
      961 accgctacac gggaggagcc tgcctcaggt gtgctcctcc cggtcgttgg tgggactcag
     1021 atggaacgag tgtcgagagg acgcgtcatg aggactcaac acatggcgga ggcggcgggg
     1081 gcctcgaaca ggccgttccc cgtagcgccc tcggttccag catcgatctg tactggctcc
     1141 ctctgggtgc aggcggtcac ttcgtccgac tgaatgggcg gatctacgaa gcgcttcacg
     1201 ccattcatga ccgccgtcgg gccttcgatc tgtatcactc cgctcttgaa gtccgggtac
     1261 cagatgggcg gtacgtcata cagatggcgt ggccaatccc cgatgcagat ggtgcgagcc
     1321 gcggcgtcgt cgtcgaaggt cccgtcggca gcagcaggat ggcgcgtctt cgcctattcc
     1381 gatacgaagt ccgccgatgg cgggacgggg tcatcccgga cgtccttgaa gccgtagcaa
     1441 gtccgcagcg cctgagcgac aacctgcact gtgcgcgcca cctcctcgac ctcgtcggtt
     1501 cggtgcgtgc gtaggtgtgg ggccgagacg aactcgggat gggcgacatg tggaactcga
     1561 actcggttgt ctcgtggctc ctcgctcgga gcggcctacc gatggaacgg atccgaccgc
     1621 ccttgggagg acgcgccccc ggctggcata tgggcatcgc tagggccata ttcgagcagc
     1681 ccgtaagcga ggaacagcgt cgctgaagaa tcgcatcatt cccggtttct cgatgcctca
     1741 tacgtgccgc atgctggttc atcagcccgg cgcgacggct gtgatcgggc gctctcctag
     1801 cgacccgccg taccactttc ggcccccgcc ccttcctatc ccgtcaaatg aaggatcgcc
     1861 tccttcccct caaattgacg gtctggtcac ccaccccgtc cgatcccggg gtaaccgccg
     1921 acgcctcggt cgaacctgcg tcaggcaagg cgaacgacga gcggcacgcc gatcggaccg
     1981 agccacgcgg cgatctgccg gatcgaccgg atcagcgacc gcagggtcat gtccacgccc
     2041 gcgccggcga ggatctcggt gaaccgctcg aggcgcgcga tgaccaagcc gcgatcggga
     2101 tcgggcatcc ggagctcggc cgcgatctcc tcgagctcgc cgcccgcatc cgagctcacg
     2161 cggtccggca gcccgagccg cgcgagcgtt ctccgcagcg aacggacggc gttcagcgcc
     2221 tgcctcgggt ccggcgagac gatcctggtt tccgagatct gcatcatcgc tgcacctcct
     2281 gctcccatcg tgatcaaccc tgatcacgaa caaggtccga gggtgcaacc agcggtcatg
     2341 cgccgcacgg accggcggca cggtcccggt gtgacctcgg tcaccctgcc gaacgctgtg
     2401 tcgatcccga gcggtctacg cgcggacgag gcggtacacg gcgctggcga accggcgggc
     2461 tcggcgcggg cggacgacgc cgccggggag ctcgaccacg aacgcgtcgg tgcgtcccag
     2521 gatgtggttc tgccaggacg tcgccgtccc gtggagcggg ccgaggtgga cgagcggcag
     2581 gccgaccagg cgcgcatagc ggcgctcgac cgtgacgtcg cccgacgcgt cgacgagcgc
     2641 gagcggctgg tggaaccaga tggtgatgtc gggctcgatc cgccggatca ggcgcatcgc
     2701 gatccgcgtc tcgcgttcgg acgcgggccg cggtcccggg tagtagcggc ctcgcggtcc
     2761 ggatcgccag tcgtagggga agttgcggtt caggtcgacg ccgcgcgcgt tctgccgcgt
     2821 gcgcgcggcc cggccgtccg ggttcaggct cggcaggatc cacaggtcga ggaaggaccg
     2881 cggccggccg gtccgcagac gtttcgcgat ccgaaccccc gcgcactcgt cgccgtggat
     2941 gcagccgacc accagcacgc ggatctcgtc gggatcgccc cgatggaaca cgcggatcgg
     3001 ccggccctgg taggactcgc cgagcagctc ccgctcatcg acggtcggtg ccgccgatgc
     3061 cgagagcgca ccgacaacgg tgatcgcgac gaccgccgcg aagacgatcg aaccccgcgc
     3121 ggctcgaggt gctctcagcc ccagaacttc caccaggggc gcttcggcct gggggcctct
     3181 ccccgctccg cggccatctc ggcggcctcc tcggcgtgga tgcgttcggc ctcgtgcgcg
     3241 agatgctcct cccgggcctg cttgtcgatc tcttccttct cggcacccca ccgctgcgct
     3301 ccgggatcgc ggctttccgt ctctcccggt ccctccatcg acatgtcggc tccttcccca
     3361 gtcggcgatg cagtctacgg ccgccctcgg agtgctgatc cgcctcggcg gccgaccggc
     3421 cgcccgaacc atcatcagcc gacgcccgag ccgacctcct cctcgatgtg ctgagggagc
     3481 ggcggcacgt acccgagcaa cggcgtgcga gtggcggcga tgtccacccc atccaccagc
     3541 gggatcatcg cgtcccggat gacgtcgacg gcggtgagat tggacgctcc ctcgcagccg
     3601 tcggaggcga cgagcacggt ggacgcgtcg agccagccga cgaccctgga cggcgcgtcg
     3661 gcgagcgtgc ggggctgctc gcctcgccgg aacaccatcg cgcgcgagtc ctcgcagctc
     3721 gacccgaccg tcagcgcgac caacccaccc cgtctcttcg gctgggtgaa gatctccgag
     3781 atcctgtcgc cggcatcccc gtgccacaga ccctggttga tcgtggggtc ggtgagatcg
     3841 agcgcgtgaa ggagcggtgc gtcgtcgcca tggaccgcgg cgtacagcag cgttcgcccg
     3901 tcggccgtga aggcgagcgc cccgaaagtc gtctccccga caccgaagac gagccgcacc
     3961 ggatcctcgc cgacgttgga cgagagccag atctcctgac cgtcgcccgt gtcgaccacg
     4021 aaggcgagcg cgaggcccga gggatggtag atcacgttca ggtaacggac gccggggacc
     4081 ggcgtgatgt cgtccgtgcg ttgggtgccg ggatagacct tgagaaggct cgtgccgctc
     4141 ggcgggatgt agacgaccgc cttgccgatc ggatgacccc acgacgagag cgggctcgtc
     4201 ggggatgtcg cgtcgcgcag gtgcttcccg tcgatcgacc ggatcgaaag cccgtccagg
     4261 agcatgcgat ccccgcgcgg gttccaggcg aacggtccgg gcgccggcgt cgggaacagg
     4321 cagctcgcgt cgttgctctc tgggtcgagc acccaggcat tgccgcccgc gacgaaggcc
     4381 acggtctgcg ctcgggcgtt gtcgcccggc ggacagccgg cggggagagg ccggccgttc
     4441 gcgaacctgg tgatgacggc cgacgacgag gagccggttc ccgagccgga ggtcgaagca
     4501 acctgggtcg atgcccccgt gcatgcggcg agcatcatga gcatgccggc gctcagcacg
     4561 cagatcgccg cacgcatcgg tctcatcggt tcgcccaccc tacgccgcgt catcgatcct
     4621 cgggcggcct caggtatcgt ccgggcgtgt ccgactcgtt cgatctcctc gtcctcggcg
     4681 acgccaaccc cgacctcgtg ttgaccggcg acgtcgagcc ggcgttcggt caggaggagc
     4741 ggctcgtcga cgcggcgcgg ctcaccgtcg gcggctccgg agcgatcgtg gcggccggcg
     4801 cggcccgtct ggggctccgg gtcgggttct gcggcgtcgt cggcgacgac gcgttcggcc
     4861 ggttcatgct cgacgagctc gaacgccgag gggtggacgt cggcggactc gtcgtcgact
     4921 cggcccggcc gaccggggtg accgtcgtgc tcgcacggcc gaacgatcgc gcgatcctca
     4981 cccatgcggg gacgatcgcg gaccttcgga ccgagcttat cgacccggcg cgtctcgaac
     5041 tcgctcggca cgtgcacgtg agctcctact tcctgcagcg gagtctcgcg cccgagctcc
     5101 cggcggtgtt cgggcgcgtt cgggccgcag gcgccaccac ttcggtcgac cccaactggg
     5161 atccgtccgg gcggtgggac ggtggccttc gcgacctgct cggccaggtc gacgtcttct
     5221 tcccgaacgc gaccgaggcc acccgcatcg cgggcatcga cgagctcgac gacgccgtcc
     5281 tcgcgctcgc cgaatgcgcc ggcgtcgtcg tcgcgaaggc aggtgccgcc ggcgccctcg
     5341 cggcccacgg cgagcggctc gtgcgcgccg cggctcccgc gatcgagacg ctcgacgcga
     5401 ccggcgccgg ggacgcgttc gacgccgggt acctggcctc gatgctcgcc ggcgatccgc
     5461 tcgagcggtc gctcgcgatc gcgaacgcgt gcggcgcgct ctcgacgcgg gccctcggcg
     5521 gcgtggacgc gcagccgacg atggaggagg tcctcgcggg gctgggcgag gactgaggtc
     5581 agctcgagat cgcccggccg ctcgcggggt cgaacaggcg gaccgcgttc ggggcgatcg
     5641 cgacgcggag ccgcgatccg gtgctcggac gttcctccgg cgggagccga acggcgacgc
     5701 tcgccctgcc gtcccgggtc tcgttcacga ggatcgcctc gtcgtcggcg gggacggagc
     5761 cgtcgagcgc cgccaccgat ccgtggacga ggacctcgtc gcccaggggt tcgaccacct
     5821 cgacgcggac gtcgaagacc gggccggcat ggccctcccc caccggacgc agaccctcgg
     5881 gccggatgcc gaccaggaca tccccgtcgg gtgccctcgc ctgctcgagc tcgacctcgc
     5941 cgaccacgac gcgccctcgc ctggccgtgc ccttcaacag gttcatcggt ggcgatccga
     6001 tgaagcccgc cacgaacacg ttggcggggt ggtcgtagac ctcctggggc gggccgacct
     6061 gctgcaggac gccctcggag agcacggcga tccgctcccc gagcgtgatc gcctcgacct
     6121 ggtcgtgggt gacgtagatc gtggtgacgc cgatgcgttc gtggagccgc ttgagctcgg
     6181 cacgcatctg gacgcgaagc ttcgcgtcca agttggagag cggctcgtcc agaaggaaca
     6241 cctcgggctc gcggacgagg gctcggccca tcgccacgcg ctggcgctgt ccgcccgaga
     6301 gctggccggg gcgacgaccg agtaagttct ccagccccag catcgtgctg acctcgtcca
     6361 gccgccgacg gacgtcctcg cgcggcatcc gccgacgccg aaggccgaac ccgaggttcc
     6421 tctccacggt catgtgcggg tagagcgcgt agttctggaa caccatggcg atgtcgcgat
     6481 ccttcggcgg cacgtcgttc acgacgcgat cgccgatcgc gatcgtcccg tcgctgatcc
     6541 gctcgaggcc cgccaccatc cgcaaggcgg tcgacttccc acacccggac ggcccgacca
     6601 ggatgaggaa ctccccgtcg gccacctcga gggtgagatc gtcgaccgcg acgaccggcc
     6661 ccgggaacac cttcgtgacg ccgtcgaacg cgacccgggc catcccgcta gcccttcaga
     6721 ccctgggtcg cgatgccgcg tacgaagtac cgctgggcga gcaggaacag cacgatcatc
     6781 ggcaccatcg acatgatgtt ggcggccgag acgaggtgcc actgcgtgtt gtgggcaccc
     6841 tggaaggtcg cgagcccgag ttgcagcgtg aacgcgtcct ggttcgtgag gatcgtcacg
     6901 agcggccaca ggaagtcgtt ccaggtccac aggaactgga tcaccgctag cgtcgcgagc
     6961 gccgggagcg acagcgggac gacgatccgc agcaggatcg acaggcggga ggcgccgtcg
     7021 atccgcgccg cctcctcgag ctcgatcggc agcgtccgga agaactgcgt gagcaggaag
     7081 atgccgaaca cgttgacgag gttaggcacg acgagcgcgc cgaggcggtc gagcagaccg
     7141 aggtccttca cgatgaggaa cgtgggtatc agcaccacct gcagcggcac catcagcgtg
     7201 gccaggatca cgaagtacac gaacgtgcgc ccgaggaaac ggatcctcgc gaaggcatac
     7261 gccgcgagcg tgcacaggac gagattgctg gcgacgacgg tcaccgtcac gaccgtggtg
     7321 ttccagaacc aggtgccgaa cgccgactcg ccgagcacct ccgcgtagtt gccgaaccgg
     7381 agcgtccccg gccacaggat cggcgggaag tggaacgtct gcgcctcggt ctcgagcgac
     7441 gtcaccacca tccacagcag cggcagtagc atcacggccg cgagcggcgc gaggaacagg
     7501 tgccaggggt cgaatggcag gaagcgacgc cgtggccgtt cgaccatccc ggtctcgccg
     7561 ggcagggacc tcggatcggc ccgggtcggt acggcggggg cctcggtgac ggcgctcatg
     7621 acgagtagtg gacgacgcgt cggcccagcc agaattgcgc cagggtcagc agcaggatca
     7681 ccaggaacag cacgtaggcg atggcggcgg cgtagccggc gagcgacagg ctcccgaggc
     7741 cctgctcgaa cgccagccgg tagacgtaga acaccacgac cgacgttcgg ccgagcggcc
     7801 cgccgtgggt cacgaagaac acctcgtcga acaactgaag ggcgctgatc gtcgaccaca
     7861 ccacgagcag tagcgtcgcc ggtccgagca tcggccaggt cacgcgccag aacgagctcc
     7921 accgactcgc cccgtcgatc gacgccgcct cgagcagctc ctgcgggacg ccctgcaggg
     7981 cggcgagata gatgatcgtg tcgaacccca gccagcccca caccgtcatc aggaccaacg
     8041 cgatcatcgc ctggttggga tcctcgaaga acccgaacgg ccccaaaccg accttgccga
     8101 gcagccagtt cgccaggccg tagttcttgt cgaacagcca caggaacatg atgcccgtcg
     8161 cgatcgtgga cgtgaccacc gggatgaaca ccgcgagacg gtagaagcgg acgaacgcga
     8221 tccggcggtt cagcgcgtgc gcgatgaaca gcgcgatcag gatcgacgcc gggacgaaca
     8281 gcgccgtgta gtagatcgtg ttggcggccg cctgccgcgc cttggggtcg ctcgcgatct
     8341 tcgcgtagtt gctgagcccc acccactcgc gctccggcga gatcaggccc gccttctgga
     8401 acgagaggat caggccccac aggatcggga tgatcccgaa gacggcgatc aggatcaccg
     8461 agggcaggat gaaggcccat ccggcggcgt gatccgagac caggaaccgc cgaaaccgat
     8521 ccaccgtcct cagcgcctcc cggcggtcag gtcgccagcc tacccggcga gcgccagcgc
     8581 atcgttcgcc tgcgccgctg cgtcgtcgag ggcggtctgc gggtccttct cgccgagcat
     8641 cgccgacacg atggcgagtc ccatcgcctc cgagacctgg ggataggccg cgagcaccgg
     8701 acgcgcctgc tcgacgttcg tcaggttctc cgcgaagacg tccacaccgg ggatcgtctt
     8761 gccgaactcg tcgatgaacg cggtgtcgtc gatcacggag aggcggaacg ggaggtggcc
     8821 cgccgccagc gcgtcggcct tcatctgctc gggtgcggtg agccaggcga cgaagtcgag
     8881 cgccgcctgc gcccgtccgt ccccgttgtc gaacaccgtc cacatgtcgg gtccggcgat
     8941 cgtctggtgg tccccgtcga acccgggaag gatctcgacg ccgtaatcga cgtcggggaa
     9001 gctcccgagg tcccacgggc ctgtcacgag catcccgatc ttgccgctgt tgaacaagcc
     9061 cgtgtacggc gagttctgca ggtcgaggaa gaccgacttg tcctggacgg ccatccccgt
     9121 gaggacctcg agggcctcga cgccctcggg cgagttgaac gccgcctggg tgttgtcctc
     9181 ggtcaggatc gagccgccgt tctgccacag gagcgggtcg tagtgccaga cggagtcctc
     9241 ggaggcgtcc atcggatacg agaacccgaa ctgcttcttc gcgggatcgg tgagcgcctt
     9301 cgcggcggcg cggaagtcgt cccacgtcca gtcggcgttc gggtactcga ggccggcatc
     9361 gtcgaacagc gtcttgttgt agacgagcgc gaggttgtcg atgagcgcgg gcaccccgag
     9421 caccctgccc tcgaaggtcg cggcgtcgcg agcgcccgcg acgaagtcct cccagtcgac
     9481 ctcggggctc tggacccagt cggtgagatc gaccaggccg ggcgcctccg cgatctgcgg
     9541 gagcgacgag ccgtactggt acgtgatgtc cggcggctcg cctccctgca gggcgaccgt
     9601 gagcttctgc agcgccttgt cgttgttgac gaacagcggg tcgatcgtga tgtcggggtt
     9661 ctcggcgttc cactggtcga gcagcgcgtt cagcgaatcc gcctcgtagt ccgtgtagcc
     9721 gtgccagaac gtgagcgtga caggaccgga cgccgtctcg ccggtggtcg cgctcttgtc
     9781 gtcgcctccc gtgcacgcgg cggcgagcag gccgagcacg acgagcacca ccaacatcct
     9841 tcgtctcatg tggaacacct ccggtcgggc attcgggaac gcatcacggc agcaccacgg
     9901 agcgcgtcag gtgcgcggga tgatcgggat ccagatcctt ggcctcggcg agcgcgatcg
     9961 ccatccggtg caccaggatg agctcggcca tcgggtcgag cgaggccacg cgcacggtgg
    10021 cgccggtcgc ccgcacgtcc tcggccacgg ccgggtcggg cgagccgagg atccatacga
    10081 gcgatcgctc gccggcgagg ctgatgggtc cgtgtcggta ttccatggct gggtacgcc
//
LOCUS       DATBRK010000477         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1140252, whole genome shotgun sequence.
ACCESSION   DATBRK010000477 DATBRK010000000
VERSION     DATBRK010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140252"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VIH70_13850"
     CDS             <1..765
                     /locus_tag="VIH70_13850"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8117491.1"
                     /translation="TGWAFVADAATFAFSAVMVGLMRTRPRARDEDERTSPWADLKEG
                     LRYVRRTTWLWVGMVAGLVSLLAVWGPWEVLVPFVVRNDLGGSAADLGLVFGAGGVGS
                     VTVALAMGQRGRLPRRAVTVLYLTWAVGMLMTAGFGLVSAVWQAMCVAFVSESCITAL
                     IVIWFTLLQRLVPGHLLGRVSSLDWLISIGGVPLSFAVVGPLASAFGADATLIGAGLI
                     GAAVTIAFMFFPGARGPERDGSLDPARDARISAPSG"
     gene            778..1755
                     /locus_tag="VIH70_13855"
     CDS             778..1755
                     /locus_tag="VIH70_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8117492.1"
                     /translation="MKYRHLGRLGLKVSPLCLGTMNFGPRTSETDSHAIMDRALELGI
                     NFFDTANVYGQRTGVGTTETIVGNWFAKGGGRRDKVVLATKVYSEMGSWPNESRLSAL
                     HIRKACEDSLRRLQTDHLDIYQMHHVYREATWDEIWQALETLVQQGKVVYVGSSNFAG
                     WHIAKANEAARDRHFLGLVSEQCIYNLAERTVELEVLPACEDYGMGVIPWSPLWRGLL
                     AGVLDGGGARGTRRASSMVQDKLAEHRAQIEAYEKLCAELGQPPAEVALAWLLANPVV
                     TAPIIGPRVPEQLEGAMLALDLRLDDAAMKRLDEIWPGPGGPAPEAFAW"
     gene            complement(1768..2136)
                     /locus_tag="VIH70_13860"
     CDS             complement(1768..2136)
                     /locus_tag="VIH70_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117493.1"
                     /translation="MSGTNEAWNEVGERFASWGRHVAERYKESSGSARESAQESQRKL
                     EDAARDITDALDRAFTALGDTIRDQQAKDDLKQAVRALGDAVSVTVAETTERIRHGVG
                     SSSPDPTADSKDDEPGPTSA"
     gene            2235..2405
                     /locus_tag="VIH70_13865"
     CDS             2235..2405
                     /locus_tag="VIH70_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HEY8117494.1"
                     /translation="MAEDRADDLAHLTDEERAVLVKVVEVLRKLRFGTVLLVVQDGKV
                     VQIEMAEKIRLR"
     gene            2586..3674
                     /locus_tag="VIH70_13870"
     CDS             2586..3674
                     /locus_tag="VIH70_13870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8117495.1"
                     /translation="MRSRLAFRLSIVLAVFGLIAAACSSDDPGTASGTSPVAADCTPA
                     ESPIVTVTAYSNVYDVYGKLISTFQSDWKESHNDQQIQFSTSFGGSTTQAQNVIAGFP
                     ADVAALSLYPDIQLIEDAGLITHDPTQDPKGGIVASSAVVFDVRPGNPLNIDNWDDLA
                     KPGVEILTPDPASSGGARWNIVAAYGAAMRGEVPGYPANDPAAAQRLLTDIFENVTVM
                     DKSAHDSIKNFQAGNGDVAITYEYAVLAAQNSGLEDEMVIPPSTVAIQTPVSVIDANA
                     EAHCVQDVAAAFVDFLHSDEAKELFLTVGFERSVDLKAAQAGDDGAYQPIDDLFTTDD
                     IGGWDELQNDTVFGPDGAFTKALAAAQG"
     gene            3678..>3880
                     /locus_tag="VIH70_13875"
     CDS             3678..>3880
                     /locus_tag="VIH70_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter"
                     /protein_id="HEY8117496.1"
                     /translation="MSTQTLERPAAPPPASPGAGRRPSVAIRSALARWGLRGTALLYL
                     GLIVALPTVAVITRGFQDGLQSLR"
BASE COUNT          574 a         1326 c         1363 g          617 t
ORIGIN      
        1 accggatggg cgttcgtcgc cgacgcggcc accttcgcgt tctcggcggt gatggtgggg
       61 ctcatgcgga cgcgcccgcg ggcccgagat gaggacgaac gcacgtcgcc ctgggcagac
      121 ctgaaggagg ggctccgtta cgtgcggcgg acgacctggc tctgggtggg gatggtggcc
      181 ggcctggtga gcctgctggc cgtgtggggg ccgtgggagg tcctcgtgcc cttcgtggtg
      241 cgcaacgacc tgggcggttc ggcggcggac ctcgggttgg tgttcggcgc gggcggcgtg
      301 gggtcggtca ccgtggcgct cgcgatgggg cagcgggggc gcctgccccg gcgcgcggtg
      361 accgtcctgt acctgacgtg ggcggtgggg atgctgatga cggcggggtt cgggctggtc
      421 tcggccgtgt ggcaggcgat gtgcgtcgcg ttcgtctccg agagctgcat caccgcgctc
      481 atcgtgatct ggttcacgct gctgcagcgg ctggtccccg gccacctcct cggacgcgtc
      541 tcgagcctgg actggctgat ctcgatcgga ggcgtgccgc tcagcttcgc ggtggtgggt
      601 ccgctcgcgt cggcgttcgg cgcggacgcg accttgatcg gcgccggcct gatcggagcg
      661 gcggtgacga tcgcgttcat gttcttcccc ggggcgcgcg gacccgagcg cgacggctcg
      721 ctggatcccg ctcgcgacgc ccggatttcc gccccgtccg gctagactcg gacgcggatg
      781 aagtaccgac acctcggtcg tctcgggttg aaggtgagcc cgctgtgcct gggcacgatg
      841 aacttcgggc cgcggacgag cgagaccgac agccatgcga tcatggaccg ggcgctcgag
      901 ctcgggatca acttcttcga caccgcgaac gtctacgggc agcgcacggg cgtcgggacg
      961 accgagacga tcgtcgggaa ctggttcgcc aagggcggcg gtcgccgcga caaggtcgtg
     1021 ctcgcgacga aggtctacag cgagatgggc tcgtggccga acgagtcgcg cctgtcggcg
     1081 ctccacatcc gcaaggcgtg cgaggacagc ctgcggcggc tgcagaccga ccacctcgac
     1141 atctatcaga tgcaccacgt gtaccgcgag gcgacgtggg acgagatctg gcaggcgctc
     1201 gagacgctcg tgcagcaggg caaggtcgtc tacgtggggt cgagcaactt tgccggctgg
     1261 cacatcgcga aggcgaacga ggccgcgagg gatcgtcatt tcctcgggct ggtgtccgaa
     1321 cagtgcatct acaacctcgc cgagcgcacc gtcgagctcg aggtgctccc cgcgtgcgag
     1381 gactacggca tgggcgtgat cccctggagc ccgctgtggc gcggcctgct ggcgggcgtc
     1441 ctcgacggcg ggggcgcgag gggaacgcga cgagcgtcga gcatggtgca ggacaagctc
     1501 gccgagcacc gcgcccagat cgaggcgtac gagaagctct gcgccgagct cggccagccg
     1561 ccggcggagg tcgcgctcgc gtggctgctc gccaatcccg tcgtcaccgc gccgatcatc
     1621 ggcccgcggg tccccgagca gctcgagggc gcgatgctgg cgctggacct gaggctcgac
     1681 gatgcggcga tgaagcggct cgacgagatc tggccgggtc cgggcgggcc cgcgccggaa
     1741 gcgttcgcct ggtagcggcg ccggatccta cgccgaggtc ggccccggct cgtcgtcctt
     1801 cgaatcggcg gtcggatccg ggctcgacga gccgacgcca tgtcggatcc gttcggtcgt
     1861 ctcggccacg gtgaccgaga cggcgtcgcc cagcgcacga accgcctgct tcagatcatc
     1921 cttggcctgc tggtcccgga tcgtgtcccc gagcgccgtg aaggcccgat cgagcgcgtc
     1981 ggtgatgtct cgcgcggcat cctccagctt gcgctggctc tcctgtgccg actcccgagc
     2041 ggaaccgctc gactccttgt accgctcggc gacgtggcgc ccccacgacg cgaaccgctc
     2101 gccgacctcg ttccatgcct cgttcgtacc ggacatcgcg atccctccgt tccgatccgg
     2161 tgccgacccg agtctaggcc ctgcacgtga agaagggtct tccccgaacg cccctggggt
     2221 aaggtttgcc cgccatggcc gaggaccgtg ccgatgacct cgcgcatctc acggacgagg
     2281 agcgtgcggt cctggtgaaa gtcgtcgagg tccttcgaaa gctgaggttc gggaccgtcc
     2341 tcctggtcgt ccaggacggc aaggtcgtcc agatcgagat ggccgaaaag atccgcctcc
     2401 ggtaggcacc gaaccgcacc aggttggttg acagggcccg gggggcattg atatccatag
     2461 ccccgttcgg ctgaccgtac ccacggaggc cgtgtcccac gacgttccgt cctttcgatc
     2521 gaccggtcca ccggagatcg agccgcagtt cgactcccaa tcggtccctc gaaaggagca
     2581 cacctttgcg gtctcgtctc gccttccgac tgtccatcgt gctcgcggtc ttcggcctga
     2641 ttgccgccgc ttgctcgagc gacgaccccg gcaccgcctc gggcacgtcc ccggtcgccg
     2701 ccgactgcac cccggcggag tcgccgatcg tcaccgtcac ggcgtacagc aacgtgtacg
     2761 acgtctacgg caagctgatc tcgaccttcc agtcggactg gaaggagtcg cacaacgacc
     2821 agcagatcca attctcgacc tcgttcggcg gcagcacgac gcaggcgcag aacgtgatcg
     2881 ccgggttccc cgccgacgtg gcggcgctct ccctctaccc cgacatccag ctgatcgagg
     2941 acgccgggct catcactcac gaccccacgc aggatcccaa gggcggcatc gtcgcctcgt
     3001 cggccgtcgt gttcgacgtt cggccgggca acccactgaa catcgacaac tgggacgacc
     3061 tcgccaagcc gggcgtcgag atcctgacgc ccgaccccgc ctcgagcggc ggcgcgaggt
     3121 ggaacatcgt ggccgcctac ggcgcggcga tgcggggcga ggtccccggg tacccggcca
     3181 acgacccggc ggcggcgcag cggctgctca ccgatatctt cgagaacgtc acggtgatgg
     3241 acaagagcgc tcacgactcc atcaagaact tccaggcggg caacggcgac gtcgcgatca
     3301 cctacgagta cgcggtcctc gccgcccaga actccggtct ggaggacgag atggtgatcc
     3361 cgccctcgac cgtcgcgatc cagacaccgg tcagtgtgat cgacgcgaac gccgaggcgc
     3421 actgcgtgca ggacgtcgcc gccgcgttcg tggactttct gcactcggac gaggcgaagg
     3481 agctcttcct gaccgtcggg ttcgagcggt cggtcgatct gaaggcggcg caggccggtg
     3541 acgacggcgc gtaccagccg atcgacgacc tgttcacgac ggacgacatc ggcggctggg
     3601 acgagctgca gaacgacacg gtgttcggac cggacggcgc cttcaccaag gccctggccg
     3661 ccgcacaggg gtagcgcttg agcacacaga cactggaacg ccccgccgcc ccgccgccgg
     3721 cttcgccggg ggcggggcgg cgtccgtcgg tcgcgatccg gagcgcactc gcgcgctggg
     3781 ggctgcgggg gacggcgctg ctctacctcg gattgatcgt cgccctgccg accgtggcgg
     3841 tgatcacccg cggattccag gacggcctcc agtccctccg
//
LOCUS       DATBRK010000478         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2010709, whole genome shotgun sequence.
ACCESSION   DATBRK010000478 DATBRK010000000
VERSION     DATBRK010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010709"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..163)
                     /locus_tag="VIH70_13880"
     tRNA            complement(89..163)
                     /locus_tag="VIH70_13880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Ile,seq:gat)
     gene            complement(295..747)
                     /locus_tag="VIH70_13885"
     CDS             complement(295..747)
                     /locus_tag="VIH70_13885"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEY8117497.1"
                     /translation="MSDPVDQATFAPPAPPPAGAPARPEPRRTKPSPARSRKARVVLR
                     KVEPWSVFKISFFFYLCVMVVILGAMMILYAILGAIGALDSLTRLIRDLFADQSFEIH
                     GGWLFTRGLSIGFVLVVLWTLINVFIVFLYNLLSDVVGGVEVTLSERR"
     gene            complement(763..1050)
                     /locus_tag="VIH70_13890"
     CDS             complement(763..1050)
                     /locus_tag="VIH70_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117498.1"
                     /translation="MSTGAGVATIVGASALGLLTVFVLWRRLPAAAAFAVITACGVGV
                     AVGGLLVQDDVGTASWVVAVVVLGVLAPVHARLVFGPPGRAHVVAARPPAA"
     gene            1080..2336
                     /locus_tag="VIH70_13895"
     CDS             1080..2336
                     /locus_tag="VIH70_13895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8117499.1"
                     /translation="MDPTTPLEHDLRIILAIQSLRAFGYGFASVVLGAALAHGGLSDG
                     QVGLVFAAILAGNALVSIAIGLAGDRIGRRRAYVLLLFVMSAAGVAYALSDSVVVLVL
                     VALTGTLSTDANESGPLTSLEQAMMGGAAPGVRARVFGRYNAIAYLSGAIGALAGGGP
                     ALIRERIPNLPTDQRWLLVLTVAGAVCVALTRRLSPAIDARAGRVTASLRRSRTEVSR
                     LAVLFGLDAFAGGFVVSTFLVFWFGRRFGASAELMGLVLFCSGLLQALSSIASGWLAT
                     RIGLLPTMVFTHLPSNVLLILIPAMPTLGSAIAMLFLRSTLSQMDVPARQAYVTALVD
                     PEERTAAAAFTNTARYVVRPFGAAGAGLLMQSVAVGAPFVVAGVLKSLYDVLVFARFR
                     RVPLPEEHPGGRTEPETETSAEPPGP"
     gene            complement(2337..>3663)
                     /locus_tag="VIH70_13900"
     CDS             complement(2337..>3663)
                     /locus_tag="VIH70_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY8117500.1"
                     /translation="AEERDHIVKGLLIALDNLDAVIKVIRGSQDAEEARGKLMSQFKL
                     SEVQANHILDMPLRRLTRLARAELEQEHKDLLARIRHLKALLKDPKKIRALITDELLE
                     IKKKFANPRRTQLKADEGDLDVQDLIAEEAVVITVSRAGYVKRQPIENFRRQGRGGRG
                     IRGANLKEEDVVNDVFTTTTHNWLLFFTSKGKVYRVKVHEVPESGRTARGTYAANLPG
                     VSITGDEKVQAVIDLKEYAEGRYLLFATRKGMVKKTTLPEYDSPRTGLAAINLRPGDE
                     LIDVRLTDGKDDVILVSRTGQAIRFKESLVRPMGRQTAGVIGMRLAPDDEVIVLGVAS
                     QGEELISVTHQGYGKRTPLKDYPVKGRGGKGVIGHTLTKKTGLLAGAFVGSKDQDMFV
                     ISSSGIVIRVAAADIRRVGRASQGVRTMRVEEGATVVALAPVIATAELE"
BASE COUNT          509 a         1293 c         1242 g          619 t
ORIGIN      
        1 tgaaagcgat cttcatcagc ctgccgaaga agccgcgctt cttcgtttcc tcagttcctt
       61 cggacatcgc cgcctcctcc tcgccgggtg ggcctggttg gacttgaacc aacgacctca
      121 tccttatcag ggatgcgctc taaccgagct gagctacagg cccaggggac actgcagggg
      181 cctcacccgg cccgacggca agcgtacgcg tggctccgaa acggtgtcaa tcgtgcgcgc
      241 gatcagggtg gattgatgga gagatcaggg tggattgatg gagagtggag cgcgctaccg
      301 gcgttcggac agcgtgacct cgacgccgcc gacgacgtcg gagagcaggt tgtacaggaa
      361 cacgatgaag acgttgatca gcgtccacag cacgacgagc acgaacccga tcgacaggcc
      421 gcgggtgaac agccacccac cgtggatctc gaaggactgg tccgcgaaca ggtcccggat
      481 caagcgcgtc agcgaatcga gcgcgccgat cgcccccaag atcgcgtaca ggatcatcat
      541 cgcgccgagg atcaccacca tcacgcacag gtagaagaag aacgagatct tgaagaccga
      601 ccacggctcg accttgcgca gcacgaccct ggccttccgc gagcgggcgg gcgagggttt
      661 cgtgcgtctg ggctcggggc gggcgggagc ccccgcgggc gggggcgcgg gcggggcgaa
      721 ggtggcctgg tcgacgggat cgctcacggg ttctggctct ttctacgcgg cggggggccg
      781 cgcggcaacc acgtgggccc gccccggcgg cccgaacacg agccgcgcgt gcacgggggc
      841 gagcacgccg agcacgacga ccgcgaccac ccacgacgcc gtcccgacgt cgtcctggac
      901 cagcaggccc ccgacggcca cgccgacccc gcacgcggtg atcaccgcga acgcggccgc
      961 cgccggcaac cggcgccaga gtacgaacac ggtcagcagg ccgagcgcgc tcgccccgac
     1021 gatcgtcgcg acaccggcac cggtcgacac gatgggatta tctgccgagg acgactaccg
     1081 tggatcccac gaccccgctc gagcacgacc tgcggatcat cctcgcgatc cagtccctgc
     1141 gcgcgttcgg ctacgggttc gcgagcgtgg tcctgggcgc ggcgctcgcc cacggcggcc
     1201 tctccgacgg ccaggtcggg ctcgtgttcg cggcgatcct cgccggcaac gccctggtct
     1261 cgatcgcgat cggtctcgcc ggagatcgca tcgggcggcg gcgcgcgtac gtcctgctgc
     1321 tgttcgtgat gagcgcggcc ggcgtcgcgt acgcgctctc cgactccgtg gtggtgctgg
     1381 tgctcgtggc gctcaccggc accctgtcga ccgacgcgaa cgaatcgggg ccgctcacct
     1441 ctctcgaaca ggcgatgatg ggcggcgccg cgccgggcgt ccgcgcccgc gtcttcggcc
     1501 ggtacaacgc gatcgcctac ctgtccggag cgatcggggc gctcgccggc ggcggcccgg
     1561 cgctgatccg cgaacggatc ccgaacctgc cgaccgacca gcgctggctc ctcgtgctca
     1621 cggtcgccgg agccgtgtgc gtcgcgctca cccgccggct ctcgcccgcg atcgatgccc
     1681 gggccggacg ggtgaccgcc tcgttgcgcc gatcgcgcac ggaggtgagc cggctcgcgg
     1741 tgctgttcgg tctggacgcg ttcgccgggg ggttcgtggt ctcgacgttc ctggtgttct
     1801 ggttcgggcg gcggttcggg gcgagcgcgg agctcatggg cctggtcctg ttctgttcgg
     1861 gcctgctcca ggcgctctcg tcgatcgcgt cgggttggct cgccacccgg atcggcctcc
     1921 tcccgacgat ggtgttcacc cacctgccgt cgaacgtcct gctgatcctg atcccggcga
     1981 tgccgacgct cggctcggcg atcgcgatgc tgttcctgcg ctcgaccctg tcgcagatgg
     2041 acgtcccggc tcgacaggcc tacgtcaccg cgctggtcga tcccgaggaa cgcaccgccg
     2101 cggcggcgtt caccaacacc gcgcgctacg tggtgcgtcc cttcggcgca gccggcgcgg
     2161 gcctgctcat gcagtcggtc gcggtcggtg cgccgttcgt ggtggcgggg gtcttgaaga
     2221 gcctgtacga cgtgctggtg ttcgcgcggt tccggcgggt gccgctcccc gaggagcatc
     2281 ccggcggccg aacggagccg gagacggaga cctccgcgga accaccgggc ccgtagctac
     2341 tcgagctccg cggtggcgat gaccggcgcg agcgcgacga cggtcgcgcc ctcctcgacg
     2401 cgcatcgtgc gaacgccctg cgacgcgcgg ccgacacggc ggatgtccgc cgccgcgacc
     2461 ctgatcacga tcccggagga cgagatcacg aacatgtcct ggtccttcga cccgacgaac
     2521 gcgccggcga gcaggcccgt cttcttcgtg agcgtgtggc cgatcacgcc cttgcctccg
     2581 cgccccttca ccgggtagtc cttgaggggc gtccgcttgc cgtacccctg gtgcgtgacg
     2641 ctgatcagct cctcgccctg cgatgcgacg ccgaggacga tcacctcgtc gtccggcgcc
     2701 aggcgcatcc cgatcacgcc cgcggtctgc cggcccatcg gtcgcacgag gctctccttg
     2761 aaccggatcg cctggcccgt gcgggagacc aggatcacgt cgtccttgcc gtcggtcaga
     2821 cggacgtcga tcagctcgtc gccgggcctc aggttgatcg cggcgagccc ggtccgcggc
     2881 gagtcgtact cgggcagcgt cgtcttcttc accatcccct tgcgcgtggc gaacaggagg
     2941 taacggccct ccgcgtactc cttgaggtcg atcacggcct ggaccttttc gtcccccgtg
     3001 atcgagaccc cgggcaggtt cgctgcgtag gtccctcgcg cggtccgtcc ggactccggc
     3061 acctcgtgga ccttcacgcg gtagaccttc cccttgctgg tgaagaacag cagccagttg
     3121 tgcgtcgtcg tcgtgaagac gtcgttgacc acgtcctctt ccttgaggtt cgcgccgcgg
     3181 atgcccctgc cgcctcgccc ctgtcggcgg aagttctcga tgggttggcg cttcacgtag
     3241 ccggcgcgtg acacggtgat gaccacggcc tcctcggcga tcaggtcctg cacgtcgagg
     3301 tcgccctcgt cggccttgag ctgcgtacgc cgggggttcg cgaacttctt cttgatctcg
     3361 agcagctcgt ccgtgatgag ggctcggatc ttcttcgggt ccttgagcag cgccttcagg
     3421 tgtcggatcc gcgcgagcag gtccttgtgc tcctgctcga gctccgccct cgcaagccgg
     3481 gtgaggcgtc ggagcggcat gtccaggatg tggttcgcct gaacctcgct gagcttgaac
     3541 tgcgacatca gcttgccgcg cgcctcctcg gcgtcctgcg aaccacggat caccttgatg
     3601 accgcgtcga ggttgtcgag cgcgatcagc aggcccttga cgatgtggtc gcgttcctcg
     3661 gcc
//
LOCUS       DATBRK010000479         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_992272, whole genome shotgun sequence.
ACCESSION   DATBRK010000479 DATBRK010000000
VERSION     DATBRK010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992272"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VIH70_13905"
     CDS             complement(<1..430)
                     /locus_tag="VIH70_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8117501.1"
                     /translation="MIRVAALGDLHVGPDSAGALREQLSAIHDKADVLLIAGDLTKSG
                     ETEEAEALARELQGLSVQVVAVLGNHDHHADRPAEIAGLLREVGVAVLEGDAVRIDTP
                     GGSLGVAGVKGFGGGFSGASGAEFGEPEMKAFIRHTKERSE"
     gene            complement(427..681)
                     /locus_tag="VIH70_13910"
     CDS             complement(427..681)
                     /locus_tag="VIH70_13910"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8117502.1"
                     /translation="MSSDESEYVAAHVQEALARDPRVNEPELGVTVVKGRVVVHGHVP
                     SEERRRSVTEVVSECCPDLEVENQTTVARFPDAGGMETVQ"
     gene            complement(678..1319)
                     /locus_tag="VIH70_13915"
     CDS             complement(678..1319)
                     /locus_tag="VIH70_13915"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEY8117503.1"
                     /translation="MSLPETAIEVTEADDGDLFLRVLDEALDALGKAGIPFLLIGGVG
                     SAVYGRDQGTRDIDVFVRPEMARRVLEVLGARGFDTKEVAERWLSKAMKHGVLVDVIF
                     RSSRDILLGDEMLARARVMPFRGRMVPIAPPEDLIVMKACAMSEDTSRYWYDAVSIIA
                     HTELDWDYLVARAREHGARRLLSLLLFATSLDIVVPSEPIETLHDAIGRGRQA"
     gene            complement(1399..2436)
                     /locus_tag="VIH70_13920"
     CDS             complement(1399..2436)
                     /locus_tag="VIH70_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEY8117504.1"
                     /translation="MTRLRRVSSDDPGIGRQRRGRGFSYLDQTGARVTDDDTIRRIRA
                     LAVPPAWLDVWICPDPLGHLQASGTDAAGRRQYLYHERWRARRDAEKFDRMLAFAHRL
                     PWVRERVAADLGRRGLPREKALATAVRLLDLALFRVGSESYTRDNGSFGLATVRRDQV
                     RTSGEVIRFDYRAKSGQRRIQEVADPEIAPIVRTLKRRRDENPELLAYRNGSGWRDVR
                     SEDVNAHVKELAGDGFSAKDFRTWHGTVFAAVALAAAGEVPVAVADRRRRISAAVKEV
                     AAELGNTPAVARASYIDPRLLDRYEEGVTITASLAEAVGGDLADAAFRDEVEAAVLDL
                     LEDGRRRLAAA"
     gene            complement(2502..>2799)
                     /locus_tag="VIH70_13925"
     CDS             complement(2502..>2799)
                     /locus_tag="VIH70_13925"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEY8117505.1"
                     /translation="ATFVGTTIATGFFMAPTAYHRIRFRQGDKERMLRTSNRFAIVGI
                     VFLALSVTSAVVLTTDLMFGLGTAAVVGLAVLLFLAWVWFAIPLARRLRDEGGH"
BASE COUNT          414 a         1054 c          943 g          388 t
ORIGIN      
        1 cctccgaacg ctccttcgtg tggcggatga acgccttcat ctccggctcg ccgaactcgg
       61 cgccgctcgc ccccgagaat ccgccgccga accctttgac gccggcgacc ccgagcgacc
      121 cgccgggggt gtcgatccgg accgcgtcgc cctccagaac ggcgacgccg acctcgcgca
      181 gcagcccggc gatctcggcg gggcggtcgg cgtgatgatc gtggttcccg agcaccgcca
      241 ccacctggac cgacaggccc tggagctcgc gcgcgagggc ctcggcctcc tccgtctcac
      301 ccgacttggt caggtcgccc gcgatcagca gcacgtcggc cttgtcgtgg atcgcgctca
      361 gctgctcgcg cagggcgccg gccgagtccg gcccgacgtg caggtcgccg agggcggcga
      421 cgcggatcac tggacggtct ccatcccccc ggcgtcgggg aaccgggcca cggtcgtctg
      481 gttctcgacc tcgagatcag ggcagcactc gctcacgacc tcggtgaccg aacgccggcg
      541 ctcctcgctc ggcacgtggc cgtggacgac cacccgtccc ttcaccacgg tgacgccgag
      601 ctccggctcg ttcacccgtg ggtctcgagc cagcgcctcc tggacgtgcg ccgcgacgta
      661 ctcggattcg tccgagctca tgcctgcctc cccctgccga tggcgtcgtg gagcgtctcg
      721 atcggctccg agggaacgac gatgtccagg gaggtggcga acagcaacag gctgagcagc
      781 ctccgcgcgc cgtgctcacg cgctcgcgcg accaggtagt cccagtcgag ctcggtgtgc
      841 gcgatgatcg agaccgcgtc gtaccagtac cgcgaggtgt cctcgctcat cgcgcaggcc
      901 ttcatgacga tcagatcctc gggcggtgcg atcgggacca tgcggccgcg gaacggcatg
      961 acccgcgccc gggcgagcat ctcgtcgccg agcaggatgt cccggctcga gcggaagatc
     1021 acgtcgacga gcacaccgtg cttcatcgcc ttggacaacc agcgctcggc gacctccttc
     1081 gtgtcgaatc cccgcgcacc gagcacctcg agcaccctgc gcgccatctc ggggcggacg
     1141 aagacgtcga tgtcccgcgt cccctggtcg cgtccgtaca ccgccgaccc gacgccaccg
     1201 atcagcagga acgggatgcc ggccttgccg agcgcatcca gcgcctcgtc caggacgcgc
     1261 aggaacagat cgccgtcgtc tgcctcggtg acctcgatcg cggtttcggg gaggctcatc
     1321 cgggcactcc tccatggccg tggcccgtcg tcgggggcac tgttcctttc ccgccgatgc
     1381 ctgcccgcaa acgggggctc aggcggcggc cagccggcgg cgaccgtcct ccagcaggtc
     1441 gaggacggcg gcctcgacct cgtctcggaa cgccgcgtcg gcgagatcgc ccccgaccgc
     1501 ctccgcgagg ctcgcggtga tcgtgacgcc ctcctcgtac cggtcgagca gcctcgggtc
     1561 gatgtaggag gcgcgcgcga ccgcgggcgt gttgccgagc tccgcggcga cctccttcac
     1621 ggccgcggag atccgacggc gtcgatccgc caccgcgacc ggcacctctc cggccgcggc
     1681 gagcgccacc gccgcgaaca ccgtcccatg ccaggtccgg aagtccttgg cggagaagcc
     1741 gtcgccggcg agctccttca cgtgcgcgtt gacgtcctcc gaacgcacgt cgcgccagcc
     1801 ggagccgttg cggtacgcga ggagctcggg attctcgtcg cggcggcgct tcagcgttcg
     1861 gacgatgggg gcgatctcgg gatcggccac ctcctggatc cgacgctggc cgctcttggc
     1921 gcggtagtcg aaccggatga cctcgcccga cgtccggacc tggtcgcgcc gcacggtcgc
     1981 gagcccgaac gacccgttgt cccgcgtgta cgactccgag cccacccgga acagggcgag
     2041 atccagcaat cgcacggccg tggcgagggc cttctcgcgc ggcaggccac gccgcccgag
     2101 gtcggcggcc acgcgctcgc gcacccacgg caaccggtgc gcgaaggcca gcatccgatc
     2161 gaacttctcc gcgtcccgac gggcccgcca ccgctcgtgg tacaggtact ggcgcctgcc
     2221 ggcggcgtcg gtgcccgacg cctgcaggtg tccaaggggg tcggggcaga tccacacgtc
     2281 caaccacgcc ggcgggaccg ccagggcgcg gatccgccgg atcgtgtcgt cgtcggtcac
     2341 acgcgctccg gtctgatcga ggtaggagaa gccccgacca cggcgctgcc gcccgatccc
     2401 cggatcgtcg ctcgacaccc gccgcagtcg cgtcatcgcc cggaggtaat gccccaggaa
     2461 gacgccccca aacgcgcgag ggtgcgggcg aacggtgtgg atcagtggcc gccctcgtcc
     2521 cgaagcctgc gcgccagcgg gatcgcgaac cagacccagg cgaggaagag caaaacggcg
     2581 agcccgacca ccgcggcggt gcccagcccg aacatcaggt cggtcgtgag caccacggcc
     2641 gacgtgacgg acaaggccag gaagacgatc ccgacgatcg cgaaccggtt cgacgtccgg
     2701 agcatccgct ccttgtcgcc ctggcggaac cggatccggt ggtaggcggt cggcgccatg
     2761 aagaagcccg tggcgatcgt ggtcccgacg aacgtcgcg
//
LOCUS       DATBRK010000480         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1731863, whole genome shotgun sequence.
ACCESSION   DATBRK010000480 DATBRK010000000
VERSION     DATBRK010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731863"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VIH70_13930"
     CDS             complement(<1..672)
                     /locus_tag="VIH70_13930"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEY8117506.1"
                     /translation="MSGYRPPPGSIPTLDVPDLADLRRIHMIGIGGAGMRNLARLFLA
                     RGVGVTGSDLKDSEGVQELRALGATIATGHAEGNLGDADAVVVSSAIRDDNPELVEAR
                     RRGIPTWARAQALAAAASDRREIAVAGTHGKTTTTAMIALVLERAGLEPTYVIGGDMN
                     ESGSGANAGSGELFVTEADESDGSFLLGRPAVGVVTNIELDHVDFYVGGLEEIESAFT
                     AFAGRC"
     gene            complement(669..1772)
                     /gene="murG"
                     /locus_tag="VIH70_13935"
     CDS             complement(669..1772)
                     /gene="murG"
                     /locus_tag="VIH70_13935"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY8117507.1"
                     /translation="MRVLLAGGGTAGHVFPAIALAREVVSMGHDVRFVGTSAGQEAGL
                     VSEAGFDFVAVEARPFERRVSLGAVTAPVAAVRSVKASRPLVEAADVVVGMGGYVSVP
                     VVLAALRSRRPVVLHEQNAVPGLANRAFARPARRVALAFAEARRALPRRARTVLTGNP
                     VRAEILAVATDRERLAKEARAEFELDEGKRTMVVFGGSQGALHVNRAFAEALRVLNRS
                     DLQVMLLTGPAHVETVRASLPGGHTVTVRVQGFLERMELAYAVADAVVARAGATTIAE
                     IAVSGLPALLVPYPYATGRHQEANARALQRAGAATMVLDDELDGYVLADRIEWLLSDD
                     QRLRSMGERARAWSKPDAANALARTVLAAGDGR"
     gene            complement(1769..>2143)
                     /locus_tag="VIH70_13940"
     CDS             complement(1769..>2143)
                     /locus_tag="VIH70_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY8117508.1"
                     /translation="HTDFIFSILGEELGLLGELVVLVAFGALIFAGIRIAANARDVFG
                     RLLAAGIVSWFGLQALINLGAVTGLLPVTGVPLPFLSYGGSSLVVSLAAVGVLVSIAR
                     ASARAPGRAAARPLARVRGSAQ"
BASE COUNT          320 a          826 c          731 g          266 t
ORIGIN      
        1 gcatcgtccg gcgaacgcgg tgaacgcgct ctcgatctcc tcgagcccgc ccacgtagaa
       61 gtcgacgtga tccagctcga tgttggtgac gacgccgacg gccggacgac ccaggaggaa
      121 ggaaccgtcg ctttcgtctg cctcggtgac gaacagctca cccgaccccg cgttcgcccc
      181 gctgccggac tcgttcatgt ccccgccgat cacgtaggtc ggctccagcc ccgcgcgttc
      241 taagacgagg gcgatcatcg cggtcgtggt cgtcttgccg tgtgtccccg cgacggcgat
      301 ctcgcgccgg tcgctcgcgg cggcggcgag ggcctgcgcc ctcgcccacg tcgggatccc
      361 gcggcgtcgc gcctcgacga gttcgggatt gtcgtcgcgg atcgcgctcg agacgacgac
      421 cgcgtcggcg tcgcccaggt tcccctcggc gtgaccggtg gcgatcgtcg ccccgagcgc
      481 ccggagctcc tgcacgccct ccgagtcctt caggtccgaa cccgtcacgc cgacgccccg
      541 cgcgaggaac aggcgggcga ggttgcgcat gcccgcgccg ccgatcccga tcatgtggat
      601 gcgccggagg tcggcgaggt ccggcacgtc cagcgtcggg atcgagccgg gcggcggacg
      661 gtagccgctc atctcccgtc tcccgccgcg aggaccgtcc gcgcaagcgc gttcgcggcg
      721 tccggcttcg accaggcgcg ggcgcgctcg cccatcgatc gcaggcgctg gtcgtcggac
      781 agcagccact cgatccggtc cgcgaggacg tatccatcga gctcgtcgtc gagcaccatc
      841 gtcgcggcgc cggcgcgctg gagcgcccgg gcgttcgcct cctgatgtcg gcccgtcgca
      901 tacgggtacg ggaccagcag cgccgggagc ccgctgacgg cgatctccgc gatggtcgtc
      961 gcccctgcgc gagccacgac ggcgtcggcg accgcgtagg cgagctccat ccgttcgaga
     1021 aacccctgca cccgcaccgt cacggtgtgg cctccgggga gcgacgcccg aacggtctcc
     1081 acgtgggccg gaccggtcag cagcatcacc tgcagatccg agcggttcaa cacgcgcagc
     1141 gcctcggcga acgcgcggtt cacgtggagc gctccctggc ttcctccgaa cacgaccatc
     1201 gtccgcttgc cctcgtccag ctcgaactcg gcgcgcgcct ccttcgccag ccgctcgcga
     1261 tcggtcgcga ccgcgaggat ctcggcccgg acggggttgc cggtgagcac cgttctcgcg
     1321 cgccgcggga gagcccggcg cgcctcggcg aacgccagcg ccaccctccg cgccggccgc
     1381 gcgaaggctc ggttggcgag cccgggaacg gcgttctgct cgtgcaggac caccggccgc
     1441 ctcgatcgca gggcggcaag caccaccggg acgctcacgt agccgcccat cccgaccacg
     1501 acgtcggccg cctcgaccag cgggcgggat gccttcaccg accggacggc cgcgacgggc
     1561 gccgtcaccg ccccgagcga gacccgtcgt tcgaacgggc gcgcctcgac cgccacgaag
     1621 tcgaaccccg cctccgacac caggcctgcc tcctgtcctg cggacgtgcc gacgaagcgc
     1681 acgtcgtgac ccatcgacac gacctcccgg gcgagcgcga tcgcggggaa cacatggccg
     1741 gccgtcccgc cacctgccag gagcaccctc actgcgccga gcctcggacg cgggccagag
     1801 gacgggcagc cgcacgccca ggcgcacggg cggacgcgcg ggcgatgctc accaacacgc
     1861 cgacggccgc gagcgagacc acgagcgagg accccccgta cgagaggaac gggagcggga
     1921 cgccggtgac cgggagcagg ccggtgaccg ccccgaggtt gatcagcgcc tgcagcccga
     1981 accacgacac gatcccggcg gcgagcaacc gaccgaacac gtccctcgcg ttcgccgcga
     2041 tccggatgcc agcgaagatg agcgccccga acgcgacgag aacgacgagc tcgccgagca
     2101 ggccgagctc ctcgccgagg atcgagaaga tgaagtcggt gtg
//
LOCUS       DATBRK010000481         3443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_419203, whole genome shotgun sequence.
ACCESSION   DATBRK010000481 DATBRK010000000
VERSION     DATBRK010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419203"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VIH70_13945"
     CDS             complement(<1..526)
                     /locus_tag="VIH70_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117509.1"
                     /translation="MSDVVPEHPKAFDPRILSRREFLRRAGVAGVAMPTMAAILAACS
                     GGAQKEQTAGNGTGAGGANPYGTGGVGGAPYPLARLDAPVTWNILDDNQPIESGLPPE
                     TGATLKYLSWPYYISPSIIRAFEDKYGCTVEKTIFPDMAGGVAKLRASPDEFDVMFGV
                     QIFVIGQLIAGGLLK"
     gene            complement(593..1525)
                     /locus_tag="VIH70_13950"
     CDS             complement(593..1525)
                     /locus_tag="VIH70_13950"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEY8117510.1"
                     /translation="MNGRTTPPDEMLALLEPTMLRGLERHLSAATEWFPHEYVPYEVG
                     RNYLDEPWEDTDSALGIISRAALEVNLLTEDNLPYYHLALWEIFGRGGVWGEWVRRWT
                     AEEGRHAIVLRDYLTVTRGVDPVLLERGRMDQVSRGYYPDGNGGSRALGPLDGVAYTT
                     LQELATRIAHRNTGTFTKDPIVERLTARIATDENLHYVFYRELGKAALEIAPSEMMLA
                     INRQVTGFAMPGLDIPEFKQKAIEIAKAGIYDLRIHHDEVVLPVLFKHWKIDQVTGLT
                     DEAEQARDGIANYLSMLDAMASHYEEKRAARTSG"
     gene            1727..2182
                     /locus_tag="VIH70_13955"
     CDS             1727..2182
                     /locus_tag="VIH70_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117511.1"
                     /translation="MPTCRICGDELHPERAEKYDYCIKRSCRERAAKALTIASVGVNK
                     AADQFVVLDERTEREMAKGRFKKEPEAAGSPRRRSRMPPADTSASVPELVPSSSVRPS
                     RPRWSQAQERLALTYRAMGMTPEQIARKLGVSPYLITQILLAASSRGKR"
     gene            2231..>3443
                     /locus_tag="VIH70_13960"
     CDS             2231..>3443
                     /locus_tag="VIH70_13960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8117512.1"
                     /translation="MARTLEAVLGPDLPIAVRAYDGSRLGPADAPATLLVRSPTVFRR
                     LITAPGELGLGRAYVSGELDVEGDLYAALAVLRDRMPDVRAFGLRQWTDVLRLAGSSV
                     LRYGFGALPVPPEEARLGGVRHSKERDAAAIAHHYDVSNAFYRIVLGPSMTYSCAVFE
                     REDVTLEDAQAAKHELVSRKLGLEPGMRLLDVGCGWGGMLLHAAANHGVRAVGVTLSR
                     RQAEWAEKAVAEAGVADRVQIRYQDYRDVRDGPFDAIGSIGMFEHVGLSQLHVYFRGL
                     RRLLRPGGRLLNHGIARPPNAGRTRFQPNSFIDRYVFPDGELHEVGSVVSTMQGTGFE
                     VRHVESLREHYALTLRAWVRNLENGWDDAVSEVGPGRARIWRLYMAASALNFEAGRTQ
                     VHQVLAVRPDEG"
BASE COUNT          494 a         1199 c         1216 g          534 t
ORIGIN      
        1 gcttgagcag gccgcccgcg atgagctggc cgatgacgaa gatctgcacg ccgaacatga
       61 cgtcgaactc gtcgggcgag gcgcggagct tcgccacgcc gccggccatg tcggggaaga
      121 tcgtcttctc caccgtgcag ccgtacttgt cctcgaacgc gcggatgatg gatggcgaga
      181 tgtagtacgg ccagctcagg tacttgagcg tcgcgccggt ctccggcggc aggccgctct
      241 cgatcggctg gttgtcgtcg aggatgttcc acgtgaccgg agcatccagc cgagccagcg
      301 gatacggggc gccgccgacg ccgccggtcc catacgggtt cgcgccgccg gctccggtgc
      361 cgttcccagc cgtttgctcc ttctgcgctc cgccggagca ggccgcaagg atcgcggcca
      421 tcgtcggcat cgcgaccccc gcaacgcccg cgcgacgcag gaactctcga cgactcagga
      481 tccggggatc gaacgccttc ggatgttcgg gaaccacgtc cgacaccccc tcgcaggctc
      541 cgattccgga gtatgacaca ggctgccagc gacggttcgg ctcactgacc cctcagccgg
      601 acgtacgtgc cgcgcgcttc tcctcgtagt gcgacgccat ggcgtcgagc atcgagaggt
      661 agttcgcgat gccgtcccgg gcctgctcgg cctcgtcggt gagacccgtc acctggtcga
      721 tcttccagtg tttgaacaag acgggcagca cgacctcgtc gtggtggatc cgcaggtcgt
      781 agatccccgc cttcgcgatc tcgatcgcct tctgcttgaa ctcggggatg tcgagcccgg
      841 gcatcgcgaa ccccgtcacc tggcggttga tcgcgagcat catctcggag ggcgcgatct
      901 cgagcgccgc cttgcccagc tcgcgataga acacgtaatg caggttctcg tcggtggcga
      961 tccgggcggt gagccgctcg acgatcggat ccttcgtgaa cgtaccggtg ttgcggtggg
     1021 cgatccgtgt cgcgagctcc tgcagcgtcg tgtaggcaac cccgtcgagc gggccgagcg
     1081 cgcgggagcc gccgttcccg tcggggtagt agccgcggga gacctggtcc atgcgcccgc
     1141 gttcgagcag taccgggtcg acgccgcgcg tgacggtcag gtagtcgcgt agcacgatcg
     1201 cgtggcggcc ctcctcggcc gtccaccggc ggacccactc gccccagaca ccgccccgtc
     1261 cgaagatctc ccagagcgcc aggtggtagt agggcaggtt gtcctcggtg agcaggttca
     1321 cctccagcgc ggcgcgcgag atgatcccga gggcgctgtc ggtgtcctcc cacggctcgt
     1381 ccaggtagtt gcggccgacc tcgtagggga cgtactcgtg ggggaaccac tcggtcgcgg
     1441 cggacaggtg gcgttcgagg ccgcggagca tcgtgggctc gaggagtgcc agcatctcgt
     1501 cgggcggggt cgtacgtccg ttcatgttca ctcgtgcctc cagcgatgag ccttcagccg
     1561 agtgattggg gctgaacgcg cgcggccgac ggtttggggc cgcggacccg tccacggacg
     1621 tgggcgcccc accagtatac gcgctgcctc cgtcctcccg ggaatgtcgg cgggctgcgt
     1681 actgttgaaa acaggcccgg ccaccacccg aacccgacga ggagccatgc ccacctgcag
     1741 gatctgcggc gacgagctgc atcccgaacg agccgagaag tacgactact gcatcaagcg
     1801 ctcgtgccgc gagcgcgccg cgaaggcgct cacgatcgcc tcggtcggcg tgaacaaggc
     1861 cgccgaccag ttcgtggtgc tcgacgagcg gaccgagcgc gagatggcga agggccgctt
     1921 caagaaggag cccgaagccg cgggctcacc ccggcggcgt tcgcgaatgc cgccggcgga
     1981 tacctccgcc tcggtccccg agctggtccc ctcgtcctcc gtccggccct cccgtccgag
     2041 atggagccag gcgcaggagc ggctggcgct cacctatcgg gccatgggga tgacgcccga
     2101 acagatcgcg cgcaagctcg gcgtgagccc ctacctgatc acccagatcc tgctggcggc
     2161 ctcgagccgg ggcaagcgct gaaccaacgc cccgagcgaa ccgcctctgc cgtaccctgg
     2221 agcggtgacg gtggctcgca ccctcgaggc cgtcctcggg cccgacctcc ccatcgcggt
     2281 gcgcgcgtac gacgggagcc ggctgggccc ggccgacgcg ccggccacgc tcctggtccg
     2341 ttcgcccacc gtgttccggc gtctgatcac cgcgccgggc gagctcggcc tgggtcgcgc
     2401 gtacgtgtcg ggcgagctcg acgtggaggg cgacctctac gctgcgctcg ccgtcctccg
     2461 cgaccggatg cccgacgtgc gagccttcgg cctgcggcag tggactgacg tgctccggct
     2521 cgcgggctcg tcggtgctgc ggtatgggtt cggcgcgctc cccgtcccgc cggaggaggc
     2581 caggctgggc ggcgtccggc actcgaagga acgggacgcg gccgcgatcg cccaccacta
     2641 cgacgtctcg aacgcgttct accggatcgt gctcggcccc tcgatgacgt actcgtgcgc
     2701 cgtgttcgaa cgcgaggacg tcacgctcga ggacgcgcag gcggcgaagc acgagctcgt
     2761 ctcgcgcaag ctcggcctgg aaccggggat gcggctgctc gacgtggggt gcggctgggg
     2821 cgggatgctc cttcacgccg ccgcgaacca cggcgttcga gcggtggggg tcacgctctc
     2881 gcggcgccag gccgaatggg ccgagaaggc ggtcgccgag gccggcgtgg ccgatcgcgt
     2941 ccagatccgg taccaggact accgcgacgt gcgcgacggc ccgttcgacg cgatcggctc
     3001 gatcgggatg ttcgagcacg tggggctgtc gcagctgcac gtgtacttcc gcgggctgcg
     3061 gcggctcctc cgcccgggcg ggcggctcct gaaccacggg atcgcccggc cgccgaacgc
     3121 cggccgcacg cgcttccagc cgaacagctt catcgaccgc tacgtcttcc ccgacggcga
     3181 gctgcacgag gtggggagcg tcgtctcgac gatgcagggc acggggttcg aggtgcggca
     3241 cgtcgagagc ctgcgcgagc actacgcgct cacgctccgg gcgtgggttc gcaacctcga
     3301 gaacgggtgg gacgacgcgg tgtccgaggt cggtccgggc cgggcgcgga tctggcgcct
     3361 gtacatggcg gcctcggcgc tgaacttcga ggccggccgg acgcaggtgc accaggtcct
     3421 ggcggtccga cccgatgagg gcc
//
LOCUS       DATBRK010000482        13666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_849952, whole genome shotgun sequence.
ACCESSION   DATBRK010000482 DATBRK010000000
VERSION     DATBRK010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13666
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849952"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VIH70_13965"
     CDS             complement(<1..222)
                     /locus_tag="VIH70_13965"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY8117513.1"
                     /translation="MLLAVNVGNTNTVLGVFRGSELERQWRLSTETERTADELAVLFA
                     GLLDQTGLSFSNEITGVVISSVVPGSTTAL"
     gene            complement(307..1152)
                     /gene="panC"
                     /locus_tag="VIH70_13970"
     CDS             complement(307..1152)
                     /gene="panC"
                     /locus_tag="VIH70_13970"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY8117514.1"
                     /translation="MTRTIAATRAACDRARAEGGAVGFVPTMGALHEGHRSLMRRAKA
                     ERDVTVVSIFVNPLQFGDPSDLETYPRGEEADLLVAGREGVDLVFAPAVDEMYPDGEP
                     EVTVDPGPLGERFEGASRPGHFRGVATVVAKLFDTVGPCAAYFGEKDAQQLAVIRRMV
                     RDLSMPVEVRCCPVVREPDGLALSSRNRLLSPEQREAATCLFLALSVAAELARAGERD
                     AGKLVAAMAREIGATPQARIAYVAVVDEETFEEPPAIGRPARALVAARFGRVRLIDTL
                     LLPTA"
     gene            complement(1149..2012)
                     /locus_tag="VIH70_13975"
     CDS             complement(1149..2012)
                     /locus_tag="VIH70_13975"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEY8117515.1"
                     /translation="MDLAIVGAGRVGTSLAVLWRRAGHRIVAVAGGPATPERAARFLP
                     GVPVADAVGAATRAEIVVIATPDAVIATVCREMAEAGALGSASAVVHASGATGLDALG
                     AAASVGATTLSVHPLQTCPTVEAAVESIPGASFAVTALDEEGHALGERLARDAGGYPF
                     RIADERKPLYHAAAVFASNYVVTVTALAEELERAAGVADPAAALTPLQDATLANVRRV
                     GAAEALTGPAVRGDAITLQRNLEALAEHVPGAVRPYVTLADLALALAERSGRVPPRGR
                     EAVEEVLVRWR"
     gene            complement(2029..3288)
                     /locus_tag="VIH70_13980"
     CDS             complement(2029..3288)
                     /locus_tag="VIH70_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117516.1"
                     /translation="MEMVCFRCDWQGRTHTSRCPSCGAPLYRPDRADRARVPDAVVED
                     DEAHLGEDPTRRSTPRGAAIGIAVVLALVTVIAVDVIGSGPSPAGSAPARRESPGAGT
                     LVYLARGHGRARLWVVDLAAGVAGPGPTVPRGTAELVDVSGAASGWVGVERHTDRRNV
                     AVSVLHGVAPDAEAVGLGHGDLVAWGPGGASLVFARNGRRAPAGCAPVSISLVTVLTE
                     KVEWALDDPGFCGPILSLSRSAAATYFTAASGDRLSVYLTGSVGVPHLMFEGMRVISA
                     RPPSAFLLAPHPPMTSRGDPSSGTGTLLGWKGIGGPVTVGDGQDLLVIERILAWTADG
                     GRVALVGSVGARSGVFVLDAGSGSGNRVPRFVMPADPALDATFDAAGRLYLSTEGGVS
                     VARDGSLEGLPLPAGAPAPSGPIVWIP"
     gene            complement(3317..3817)
                     /gene="folK"
                     /locus_tag="VIH70_13985"
     CDS             complement(3317..3817)
                     /gene="folK"
                     /locus_tag="VIH70_13985"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY8117517.1"
                     /translation="MATSAFLALGSNLGDRLEHLRAAARRLDATRGIDVVRSSRVYQT
                     EPVGPPQPPYLNAVIEVVTTLLPRELLEAARGVEDSLGRVRAERWGPRTIDVDILVYN
                     EQTIDEPDLVIPHPRMHERGFVLVPLGELDADAMLPGGRRLSSHRLSADAVFEVRPFA
                     PPLPTG"
     gene            complement(3819..4166)
                     /locus_tag="VIH70_13990"
     CDS             complement(3819..4166)
                     /locus_tag="VIH70_13990"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY8117518.1"
                     /translation="MTSRLFLTGIAASGRHGANPGEKDRAQDFVVDLDVEVEVGDDEL
                     TSTADYRTLIRTARETVERDSFDLLESLAHAVANDVLSLDGVVKVSAIVHKPAAARSS
                     EVQGIAAAATVER"
     gene            complement(4163..5068)
                     /locus_tag="VIH70_13995"
     CDS             complement(4163..5068)
                     /locus_tag="VIH70_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8117519.1"
                     /translation="MTWTEKRRVATPDGELALVEAGDAEAPPVVLLSGGLTSSYLWRG
                     LIPLLSPWMRVIAPDLLGSGDSDSPEGVDLRLPAQAANVRALADGLGIERFAVVGHGR
                     GGGVAQLLAIEGGVEALVLIDSIAFDAWPSPRILELRSRVGDVDAALVRSWFDTFFDA
                     GMAHRERLSDADLQEYRRPFERADGVQRFTRIVAGFDGEGLVGLEPRLAELEIPAFVL
                     WGEDDAFLPSELAERLGDALPWASVALLPGCGHFLLEDAPETVAPLIVQWLRTQYLRL
                     PQAHAHDEEAVSVELGRRPPENGRW"
     gene            complement(5065..5913)
                     /gene="folP"
                     /locus_tag="VIH70_14000"
     CDS             complement(5065..5913)
                     /gene="folP"
                     /locus_tag="VIH70_14000"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY8117520.1"
                     /translation="MGGPIWRVGDRAFDCSERTLVMGILNVTPDSFSDGGRFLDRATA
                     VAHATLMVDDGADILDVGGESTRPGSAPVSADEELERVHPVIERLAELHPGVPISIDT
                     RKAEVAAEALDAGATIVNDVSGGADPAMFDVVRDREAAIVLMHMQGDPTTMQEAPHYD
                     DVVGEVHEYLRQRIEVAELAGIDAERIAIDPGIGFGKGLDHNLELMHGVDALLDLGRP
                     VMVGPSRKKFIGTILDLPEEERVEGTVGAVVWMVARGAHLVRVHDVKVVVRAVRVSDA
                     IAKAGA"
     gene            complement(5944..6189)
                     /locus_tag="VIH70_14005"
     CDS             complement(5944..6189)
                     /locus_tag="VIH70_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117521.1"
                     /translation="MPLPPSSGDCCGFLEADDRGIWFMSIAADGSARLLNLFDPSSGE
                     VTELATLDEGAPVAMAVAPNSVWILNSDGTLTHLAVG"
     gene            complement(6379..6603)
                     /locus_tag="VIH70_14010"
     CDS             complement(6379..6603)
                     /locus_tag="VIH70_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117522.1"
                     /translation="MRVYAMGGTGAADLAFEGGDLWVLVFGDEAVGHRMEVVRVDPES
                     GGVLARVPLNAGLGALDRRRRWTAGRPGGR"
     gene            complement(6870..7862)
                     /locus_tag="VIH70_14015"
     CDS             complement(6870..7862)
                     /locus_tag="VIH70_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117523.1"
                     /translation="MANGPIYLRVGGGEGGSRIESVEPDGSGRRVVFANDVDVHYDRI
                     SFSPDGMRIAFDNYLVDRYGIETANADGTNVARLTDGVNDSWASWSPDGTQIVFASTR
                     YDPAVEGCEPGFPHEFRCPTDIYVMDDDGSNVVRLTDDPAGEFMPRWSPDGSLIAFVR
                     EGDLAAGTYEAVYAMRPDGSDVRRVSSGDRGSDFWPSWSPDGSRIVFAAIRAEDWGIW
                     VVDADGSNERLILGGTGAGYVDNPVWSPDGSLIAFVGNLTIDDYSPDDALYVMRPDGT
                     GVTPIADAPGIGVAGDLAWQPLPVAAVTVEPVPAPTEAEVVDTLAVGQDVRSVA"
     gene            complement(8956..9477)
                     /locus_tag="VIH70_14020"
     CDS             complement(8956..9477)
                     /locus_tag="VIH70_14020"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8117524.1"
                     /translation="MFTFGPVNHPVRSRVEQAPAGTSRSEEIERLYRERGDRVWRAIL
                     AFSGDPEVASDAVAEAFAQVLRRGVEVEDPERWVWRAAFRIASGELKERRRRTVVEAM
                     KMYEMEEPARDLVVALAKLSEKQRASVVLHDAAGYPAKEIARIVGSTEAAVRVHVMRG
                     RRRLRELLNEDDD"
     gene            9535..13074
                     /locus_tag="VIH70_14025"
     CDS             9535..13074
                     /locus_tag="VIH70_14025"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8117525.1"
                     /translation="MPVICSNCGAENRRGRKFCANCGTALAVGCSVCGAANEPGDRFC
                     GECGSSLDASSSRPGEAAGLPSPVSERRLVSVLFADLVGFTSLSESRDPEEVRELLTR
                     YFDTARVLVTRYGGTIEKFIGDAVMAVWGAPVAQEDDAERAVRAGLDLVAAVADLGIE
                     VHAPELALRAGVATGEAAVTLGAEGQGMIAGDLVNTASRVQSAALAGTVLVTEPTRRA
                     TEVAIVFEEAGDHELKGKGKPIRLARAVRVIALVGGELRSVGLESPFVGRNREFRVLK
                     DQFHASAEDRKAHLVSVTGVAGIGKSRLSWEFFKYIDGLVDTTWWHRGRCIPYGEGVT
                     YWALAEMVRMRARIAEDEQPDSAGDKLRAAVEQHVKDEDDRRFVEARLAHLLGLEEPT
                     AASREDLFAGWRMFFERMAETWPVAMVFEDLQWADESLLDFIEYLLDWSRDHPVYVVT
                     LGRPDLLERRPTWGAGRRGSTSMTLEPLTPDAMDELVRGMVPGLPHELRARIRDRAEG
                     IPLYAVETVRMLLDRGLIRRGGDRFELEGPVEELAVPETLQALIAARLDGLPPDERAL
                     VQDASILGKTFTRAGLAALTGKGEDELEPLLTSLVRKELLSLQMDPRSPERGQYGFLQ
                     SLVQKIAHDRLSKRDRRARHLAAADFIERSWVGDEDEIVEVVASHLLDAYDLAPDAED
                     ATALRDRARQTLARAGRRAQSLAAMQLARGYFERAAALSDDQMEQAGLLEQAGACAVP
                     LGHMPDAVRLYERARDLYESFGADHAAARASARIGETLWLGDRADEGMSTMESALVVL
                     AEAEPDHDLALLHAALGKVRFFLGRYEGSAAELDAAIDIAEALMLPDVLSEALNTKGL
                     VAGVRGRWEEQYALLKRALELALEHDLGAAAMRAYNNLSFATMTRDRWDEAREYQEAG
                     IALGDRLGYSGFLYFLRAHLDQNRLLHGEWEPFEALIDELNAAPDATFYAGTETLSYS
                     ASLVLIARGDVASARALIDRFVGDPSTGDVQARAFRRSARAAVLIGERRYGEALGEAR
                     TVLEGAPVLGLGHDVVRFGLSHGLEAAVRIGDPEAAEELMRIVTEAPPGATGPFVHAQ
                     VDRYTAAVAAMRGDPAGAESHFKAAIGLFRELAMPFHLAMVQLEFGEWLVAQGRADEA
                     RPTLDEARSTFEELKAVPYLERIPAAAPDLAPA"
     gene            complement(13081..13653)
                     /gene="folE"
                     /locus_tag="VIH70_14030"
     CDS             complement(13081..13653)
                     /gene="folE"
                     /locus_tag="VIH70_14030"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY8117526.1"
                     /translation="MAEIDRAKIEAAVRMILEGIGDDPDREGVVDTPRRVADMYGEIF
                     EGVGVDPTPVATVVPGANFDEMIMVKDIPLQSLCEHHLLPFSGHAHVAYIPNKDGRIT
                     GLSKVARVVDILSKRPQVQERLTTEIADTIERALEPRGVFVVIEAEHLCMTMRGVKKP
                     GSITVTSAVRGLFRNDARTRQEALHHIGKG"
BASE COUNT         2086 a         4934 c         4709 g         1937 t
ORIGIN      
        1 cagggcggtc gtcgagcccg gcaccacgga ggagatgacg acgccggtga tctcgttgga
       61 gaacgagagc ccggtctggt ccaggagccc ggcgaacagc accgcgagct cgtccgccgt
      121 gcgttcggtc tcggtcgaca gtcgccactg gcgctcgagc tcggagccgc ggaacacccc
      181 gagcacggtg ttcgtgttcc cgacgttcac ggcgagcagc acccggcgcc acctccctga
      241 ccggccatcc tgccatgccg cgacgcggca cggcatccgt ccgcccacgg gccatccgtc
      301 atcggctcac gccgtgggga gcaggagcgt gtcgatcagc cgcacccggc cgaagcgggc
      361 ggcgacgagc gctcgggccg gacgcccgat cgccggcggc tcctcgaacg tctcctcgtc
      421 cacgacggcc acgtaggcga tgcgcgcctg gggcgtggcg ccgatctccc gggccatcgc
      481 ggccacgagc ttcccggcgt cgcgctcgcc ggcgcgcgcg agctcggccg ccaccgagag
      541 cgcgaggaac aggcaggtgg cggcctcgcg ctgctcgggg gacaggagcc ggttgcggct
      601 cgacagggcg agcccgtcgg gttcgcgaac gaccgggcag caccgcacct ccaccggcat
      661 cgacagatcg cgcaccatcc gccggatcac cgcgagctgc tgcgcgtcct tctcgccgaa
      721 gtacgccgcg cacggcccca ccgtgtcgaa caacttggcc acgacggtgg ccaccccgcg
      781 gaagtgaccg ggtcgcgacg caccctcgaa ccgctccccg agtggcccgg ggtcgaccgt
      841 gacctcgggc tcgccgtccg gatacatctc gtccaccgcc ggcgcgaaaa cgaggtcgac
      901 tccttcgcgt ccggcgacga ggaggtcggc ctcctcgccg cgaggatagg tctcgagatc
      961 actcggatcg ccgaactgca acgggttcac gaagatcgag acgaccgtca cgtcgcgctc
     1021 cgccttcgcc cggcgcatca gcgagcgatg gccctcgtgg agcgcaccca tcgtcgggac
     1081 gaatcccacg gccccgccct ccgcgcgcgc gcgatcgcac gccgcgcggg tggcggcgat
     1141 cgtcctggtc acctccagcg gacgagcacc tcctcgacgg cctctcggcc ccgcggggga
     1201 acgcggcccg agcgctcggc cagcgccagc gccaggtcgg cgagcgtgac gtagggacgg
     1261 accgcgccgg gcacgtgctc ggccagcgcc tcgaggttgc gctgcaaggt gatcgcgtcc
     1321 cctcgaacgg cgggtccggt cagcgcctcg gcggctccga cccgacggac gttcgcgagc
     1381 gtggcgtcct gcaggggcgt gagcgcggcg gccgggtcgg ccaccccggc cgcgcgttcg
     1441 agctcctcgg ccagggccgt cacggtgacg acgtagttgg acgcgaacac cgcggccgcg
     1501 tggtacagcg gcttgcgttc gtcggcgatg cggaacgggt atccgcccgc gtcgcgagcg
     1561 agtcgctcgc cgagcgcgtg gccctcctcg tcgagggcgg tgaccgcgaa cgacgcgccg
     1621 gggatcgact ccacggccgc ctcgaccgtg ggacacgtct gcaggggatg gaccgagagc
     1681 gtcgtggcgc cgacggacgc cgcggcgccg agcgcgtcca gaccggtcgc gccgctcgcg
     1741 tggacgacgg cgctcgcgga tcccagcgcg cccgcctcgg ccatctcccg gcacacggtg
     1801 gcgatcacgg cgtcgggcgt cgcgatcacg acgatctcgg ctctggtcgc cgccccgacc
     1861 gcatcggcga cgggaacgcc ggggaggaag cgcgcggcgc gttcgggcgt agccggtccg
     1921 ccggcgaccg cgacgatccg atggccggcg cgccgccaga gcaccgcgag cgaggtcccg
     1981 acgcggcccg cacccacgat cgcgagatcc atcttccgag tatcccgctc aggggatcca
     2041 gacgatcggt cccgacggcg ccggcgcccc cgccgggagc gggagacctt cgagcgatcc
     2101 gtcgcgtgcc accgagaccc cgccctcggt ggacaggtag aggcggccgg ccgcgtcgaa
     2161 ggtcgcgtcg agcgccggat cggccggcat gacgaaccgg ggcacccggt tgcccgaacc
     2221 cgaccccgca tcaagcacga acacgccgct ccgcgcgccg acgcttccga cgagcgccac
     2281 acggcccccg tccgccgtcc acgcgaggat ccgctcgatc acgaggaggt cctgtccgtc
     2341 gccgaccgtc accggaccgc cgatcccctt ccaccccagc agggttccgg tgccggacga
     2401 gggatcgccc ctcgaggtca tgggcgggtg cggcgcgagc aggaacgccg acggcggcct
     2461 cgccgagatc accctcatgc cctcgaacat caggtggggg accccgaccg agccggtgag
     2521 gtagacgctc agccggtcgc cggaggcggc ggtgaagtac gtggcggccg cgctccggct
     2581 cagcgagagg atcggtccgc agaaccccgg gtcgtcgagc gcccactcga ccttctcggt
     2641 gagcacggtg accaggctga tgctgaccgg cgcgcacccc gccggtgctc tgcgtccgtt
     2701 gcgggcgaac accaggctcg cgccgccggg gccccaggcc acgaggtcgc cgtgaccgag
     2761 cccgacggcc tcggcgtcgg gcgcgacgcc gtgcaggacc gagacggcga cgttccgacg
     2821 atccgtgtga cgctcgacgc cgacccatcc cgaggcggcg ccggagacgt ccaccagttc
     2881 ggcggtccct cgcggcaccg tcgggccggg cccggcgacg ccggcggcca ggtcgaccac
     2941 ccacagcctc gcccgaccgt gaccacgggc gaggtagacg agggttccgg cgccgggcga
     3001 ctcccttcgg gcgggcgccg aacccgcggg ggacggcccg ctgccgatca cgtcgaccgc
     3061 gatcaccgtc acgagggcga gcacgacggc gatcccgatg gcggcgccgc gcggggtcga
     3121 acgcctcgtc gggtcctcgc cgagatgcgc ctcgtcgtcc tcgacgaccg cgtcggggac
     3181 gcgtgcgcgg tctgcccggt ccgggcggta caggggcgcc ccgcacgatg ggcagcggct
     3241 cgtgtgggtg cggccctgcc agtcgcagcg gaagcagacc atttccatca tcttggctcc
     3301 gtcatcgttg ctcggctcat ccggtcggga gcggcggggc gaacggtcgc acctcgaaga
     3361 ccgcgtcggc ggagagtcgg tgcgacgaca ggcgtcggcc cccggggagc atcgcgtcgg
     3421 cgtcgagttc gccgaggggg accaggacga acccgcgttc gtgcatcctc ggatgtggga
     3481 tcaccaggtc gggctcgtcg atcgtctgct cgttgtacac gaggatgtcg acgtcgatcg
     3541 tccgcggccc ccagcgttcg gctcgaacgc gaccgagcga gtcctccacg ccgcgggcgg
     3601 cctcgagcag ttcgcgcggc aacagggtcg tgaccacctc gatcacggcg ttcaggtacg
     3661 gcggttgcgg cggccccacg ggttcggtct ggtagacgcg cgaggagcgg accacgtcga
     3721 tcccccgcgt cgcgtcgagc cttcgcgcgg cggcacgcag atgctcgagg cggtccccca
     3781 ggttcgagcc gagggcgagg aacgcggacg tcgccatgtc aacgctcgac cgtcgcggcc
     3841 gccgcgatgc cctggacctc gctcgaacgc gcggcggcgg gcttgtgcac gatcgcgctc
     3901 accttgacga cgccgtcgag cgagaggacg tcgttcgcga cggcgtgcgc gagcgactcg
     3961 agcaggtcga acgagtcgcg ctcgacggtc tcgcgcgcgg tacggatcag ggttcggtag
     4021 tcggcggtcg acgtgagctc gtcgtcgccc acctccacct cgacgtccag gtccacgacg
     4081 aagtcctggg cccggtcctt ctcgcccggg ttcgcgccgt ggcgtcccga ggccgcgatc
     4141 ccggtcagga acaggcgcga ggtcaccacc ggccgttctc gggcggacgt cggccgagct
     4201 ccacgctcac cgcctcctcg tcatgcgcat gcgcctgcgg gaggcggagg tactgggtgc
     4261 ggagccactg cacgatcagc ggcgcgacgg tctcgggggc atcctcgagc aggaagtggc
     4321 cgcatccggg cagaagcgcc accgaggccc agggcaacgc gtcgcccagg cgttcggcga
     4381 gctcggacgg caggaacgcg tcgtcctccc cccacaggac gaacgccggg atctcgagct
     4441 cggcgagccg gggttccagc ccgaccaggc cctcgccgtc gaagcccgcg acgatccggg
     4501 tgaagcgttg cacgccgtcg gcgcgctcga acgggcgtcg gtattcctgg aggtcggcgt
     4561 ccgagaggcg ctcgcggtgc gccatcccgg cgtcgaagaa cgtgtcgaac cacgagcgga
     4621 cgagggcggc gtcgacgtcg cccacgcgcg accggagctc gaggatcctg ggcgagggcc
     4681 atgcgtcgaa cgcgatcgaa tcgatgagca cgagcgcctc caccccgccc tcgatcgcga
     4741 ggagctgcgc gacgcctcca ccacgaccgt ggccgaccac ggcgaaccgc tcgatgccga
     4801 ggccatccgc gagggctcgg acgttcgcgg cctgggccgg gaggcgaagg tcgacgccct
     4861 cgggtgaatc ggagtcgccc gaccccagga gatcgggggc gatcacgcgc atccatggcg
     4921 acaggagcgg gatcagtccg cgccacaggt aagaggacgt gaggccgccc gacagcagca
     4981 cgacgggggg cgcctccgcg tcgccggcct ccacgagcgc gagctcgccg tccggggtcg
     5041 ccacccggcg cttctcggtc caggtcatgc gccggccttc gcgatcgcgt ccgatacccg
     5101 gaccgcgcgg acgaccacct tgacgtcgtg cacgcggacg aggtgcgctc cccgggcgac
     5161 catccacacg accgcgccca cggtgccctc gacgcgttcc tcctccggca gatcgaggat
     5221 cgttccgatg aacttcttcc gggacgggcc gaccatcacc ggccggccga ggtcgaggag
     5281 cgcgtcgacg ccgtgcatca gctcgaggtt gtggtcgagg cccttgccga acccgatccc
     5341 cggatcgatc gcgatccgct cggcgtcgat cccggcgagc tcggccacct cgatccgctg
     5401 ccggaggtac tcgtgcacct cgccgaccac atcgtcgtag tgcggggcct cctgcatcgt
     5461 ggtcggatcg ccctgcatgt gcatcaggac gatggcggcc tcgcgatctc ggacgacatc
     5521 gaacatggcc gggtcggcgc cgcccgacac gtcgttcacg atcgtggccc cggcgtccag
     5581 cgcctcggcc gcgacctccg ccttccgcgt gtcgatcgag atcgggaccc cggggtggag
     5641 ctcggcgagg cgctcgatca cgggatggac gcgctcgagc tcctcatccg ccgagaccgg
     5701 ggccgagccc gggcgggtgg attcgccgcc gacgtccagg atgtccgcgc cgtcgtcgac
     5761 catcagcgtc gcgtgcgcca ccgcggtggc tcggtcgagg aacctcccgc cgtcggagaa
     5821 cgagtccggc gtgacgttga ggatccccat gacgagcgtg cgttcggagc agtcgaacgc
     5881 ccgatcgccg acacgccaga tgggaccgcc cacgaaccca tcgtagcggt gaccgaggaa
     5941 ggctcagccg accgcgaggt gcgtcagcgt cccatcggag ttcaggatcc agaccgagtt
     6001 gggcgcgacg gccatcgcga ccggcgcgcc ctcgtccagc gtcgcgagct ccgtcacctc
     6061 cccgctcgaa gggtcgaaga ggttcagcag acgggccgat ccatccgcgg cgatcgacat
     6121 gaaccagatg ccgcgatcat cggcctcgag gaagccgcaa cagtccccgg agctcggcgg
     6181 cagcggcacg acgggctccg gaaatgcggc ggccgtcggg gcgaggcgtg cgaatccggg
     6241 atcgagcgag atccatacct gccttcgcca cacgacgggc atgggcgtga aggacctcga
     6301 gggagctcag ctcgcgagac gctgttcgtc gacggatcga cgacggccgc gcggcggccg
     6361 tccacgttca cggcgtcgtc accgccctcc aggacgacca gccgtccatc ggcggcgacg
     6421 atcgagtgcg cccagccccg cgttcagcgg aaccctcgcc agaacgccgc cgctctccgg
     6481 gtccacccgg acgacctcca tcctgtgacc gacggcttcg tcgccgaaca cgagcaccca
     6541 caggtcgccg ccctcgaacg cgaggtcggc agcgcccgtt ccgcccatcg cgtagacccg
     6601 cacgacctcg ttcgtcgatg cgtcgatcct gacgactgcc gcatccgccc ttccgccggg
     6661 gaatccagac cgccgagttt gtcccggtcg cctgcaaccc aaagatcgcc gtccgcgaac
     6721 accatcgctc cgcctccgac ctcccaggtc gggaccgccc cgatctggat ctcggcctgc
     6781 acgtcgtgcg ttgccggatc gatgcgaacg atccgtccgg cggacgatcc gtggccgttg
     6841 gagacggcaa cccatacgct cccctcccct cacgcgaccg aacggacgtc ctggccgacc
     6901 gcgagcgtgt cgacgacctc tgcctctgtt ggcgccggca cgggttcgac cgtcaccgcg
     6961 gcgaccggaa gcggctgcca ggcgaggtcg cccgcgacgc cgatccccgg cgcgtcggcg
     7021 atcggcgtga cgccggtccc gtccggacgc atcacgtaca gcgcgtcgtc ggggctgtag
     7081 tcgtcgatcg tgaggttgcc gacgaaggcg atcagcgagc cgtcaggtga ccagaccggg
     7141 ttgtcgacgt agcccgctcc cgtccccccg aggatcaagc gttcgttcga accgtccgcg
     7201 tcgacaaccc agatgcccca atcttccgct cggatcgccg cgaagacgat ccgcgaccca
     7261 tccggcgacc agctcggcca gaagtcgctg ccgcgatctc cgctcgaaac ccgacgaaca
     7321 tcggaaccgt cggggcgcat cgcgtagacg gcttcgtacg tgccggctgc gaggtcaccc
     7381 tcgcgaacga acgcaatcag actgccatcg ggtgaccacc tcggcatgaa ttcgcctgcc
     7441 ggatcgtcgg tcaggcgaac gacgttcgag ccgtcgtcgt ccatcacgta gatgtcggtc
     7501 gggcaccgga actcgtgagg gaaccccggc tcgcatccct cgacggccgg gtcgtaccgg
     7561 gtgctcgcga acacgatctg cgttccatcc ggtgaccacg acgcccacga gtcgttcacg
     7621 ccgtccgtga ggcgggcgac attggtcccg tcggcgttcg ccgtctcgat cccgtaccgg
     7681 tccacgaggt agttgtcgaa cgcgatccgc atgccgtccg gggagaacga gatgcggtcg
     7741 tagtgcacgt cgacgtcgtt ggcgaagacg acgcgccgtc cggaaccgtc gggctcgacg
     7801 gactcgatcc ggcttccgcc ttcaccaccg ccgacccgta aatagatcgg cccgttcgcc
     7861 atcggacggc ccagtgccgg cacggtctga tcagaaagga cgggctgcca cgacggagag
     7921 actgcatcta cgggcagacc gggaatcccg cgaaccccgg tgccatcgat atccatgacg
     7981 tagagcgtcg cccgttccgg cccgatcgga gccatgaaca cgatctgccg gccgtcaagc
     8041 gaccaggaag gcgactgtgc ggacggtcga tcgccgagcg gcccaaggtc ggatccatcg
     8101 gcagccaccc gccagagccc gaagctcgcc ccggccgacg tcgagcgagc gaatgcgagc
     8161 gtggacgcgt ccggagacca cgcaagatct gatgcccacc ttgcactgtc gagctcgatt
     8221 gccacgggct tggggccaga gtcctcaact ggccagatga acacgctcac ggggatgatc
     8281 gctccggcgc ccgacaccct gacgaacgcg atgcgagatc cgtccggtga ccaagccggc
     8341 gagccgtcct gaacacactc aggcggatcg cagttggtga acgcccgagg gtgcgatcca
     8401 tcggcgccga tgatccagat ctgatcggcc cgccccggag aagaatgtgt gtaggcgagc
     8461 cttgagccat cgggcgacca cgtcggaccc caactcgagc cgggagcggt ggtgagtgcg
     8521 tacgcgccgc tgccatctgc gttaatgacc cagatgtcag atgcaccgtt gctctcccgg
     8581 acgacatatg caatccgtcg gccatcagga gaccaagcag ggtcatccgc aacctgatcc
     8641 gtgagatcgg tgagcatcat aaggtgtgcg ccgtcgggct cgatcgtgta gatgttccat
     8701 gtcgcttcgc cgagcccggc gaaggcgatt ctgccatccg agacggcggc agccgtccgc
     8761 ttcgaatgct ccgaaccgct gaacgtcagc gccgccgcgc caaccccggc ggctgcaacc
     8821 gcgagggcca ccacgattgc gacagcccgc cgccgcgtcg ggggggacgg gagcggccgg
     8881 ggctcacgat cctcgatgtc gccccacagc tcggggggac gcgctcggct gagcgcctcg
     8941 aaccgatccc cgaggttagt catcgtcttc attcaggagc tcgcggagcc gccgccggcc
     9001 ccgcatcacg tgcacccgga ccgcggcctc cgtcgaaccc acgatcctgg cgatctcctt
     9061 cgccggatag ccggccgcat cgtgcagcac caccgaggca cgttgcttct cggacagctt
     9121 cgcgagcgcg accaccagat cgcgggccgg ctcctccatc tcgtacatct tcatcgcctc
     9181 gaccaccgtt cttcgcctac gctccttcaa ctcgccgctc gcgatccgga acgccgcccg
     9241 ccacacccat cgctcgggat cctcgacctc gacgcctcgt cgcaagacct gcgcgaacgc
     9301 ctccgcgacg gcgtcgctcg ccacctccgg atcgcccgag aacgccagga tcgcgcgcca
     9361 gacccggtcg ccccgctccc ggtacaggcg ctcgatctcc tccgagcgcg aggtgcccgc
     9421 cggcgcctgc tccactctcg acctcacagg gtggttgacc ggcccaaacg tgaacatggt
     9481 tccattgttc gccccctcgc ctcgggctag gctcaccccc cggacgcgac gggagtgcct
     9541 gtgatctgct cgaactgcgg ggccgagaac cgtcgggggc gcaagttctg tgcgaactgc
     9601 gggaccgccc tcgcggtggg ttgtagcgtg tgcggggccg cgaacgagcc cggggaccga
     9661 ttctgcggcg agtgcggctc ctcgctggac gcctcctcct cacgacccgg cgaggccgcc
     9721 ggcctgccct cgccggtcag cgaacgccgt ctcgtctcgg tcctcttcgc cgaccttgtg
     9781 ggattcacct cgctgtcgga gtcgcgagat cccgaggagg tgcgcgagct gctcacgcgc
     9841 tacttcgaca cggctcgggt cctcgtcacg agatacggcg gcacgatcga gaagttcatc
     9901 ggcgacgccg tgatggcggt atggggcgcc cccgtcgcgc aggaagacga cgcggagcga
     9961 gcggtgcgtg ccggcctgga cctggttgcc gccgtcgccg atctcgggat agaggtccac
    10021 gcacccgagc tcgcgctgcg ggccggcgtg gccaccgggg aggcggccgt cacgctcggc
    10081 gccgagggcc aggggatgat cgccggcgat ctcgtcaaca cggcctcccg tgtccaatcg
    10141 gcggcgttgg ccggaacggt tctggtgacc gaacccactc gacgggcgac tgaggtggcc
    10201 atcgtcttcg aggaagccgg cgaccacgag ctgaagggga agggcaaacc gatccggctc
    10261 gctcgtgcgg tccgcgtgat cgcgctggtg ggtggggagc tccgttcggt aggcctggaa
    10321 tcgccgttcg tcggacgcaa tcgcgagttc cgggtcctca aggaccagtt ccacgcctca
    10381 gccgaggatc gcaaggcaca tctcgtctcc gtcacgggcg tggcagggat cggcaagtcc
    10441 cgactttcct gggagttctt caagtacatc gacggcctcg tggacaccac gtggtggcat
    10501 cgcggccgat gcatccccta cggagagggc gtcacatact gggcgctcgc ggagatggtc
    10561 cggatgcgag cgcggatcgc cgaggacgag cagccggact cggcggggga caagctccgg
    10621 gcggccgtcg agcagcacgt caaagacgag gacgaccgcc gcttcgtcga ggcacgcctc
    10681 gcgcacctgc tgggcctgga ggaaccaacg gcggcctcac gcgaggacct gttcgctgga
    10741 tggcgcatgt tcttcgagcg gatggccgag acctggccgg tcgcgatggt cttcgaggat
    10801 ctgcagtggg ccgacgagag cctgctcgac ttcatcgagt atctgctcga ctggtcgcgc
    10861 gatcaccccg tgtacgtcgt gaccctcggc cgccccgacc tcctcgagcg ccgaccgacg
    10921 tggggggccg gccgtcgcgg ctcgacctcc atgaccctcg agccgctgac cccggacgcc
    10981 atggatgagc tcgtccgcgg gatggtgccg gggctgcccc acgagctgcg cgcgcggatc
    11041 cgcgatcgcg cggagggcat acccctgtat gcggtcgaga cagtgcgcat gctcctggac
    11101 cgtggcctca tccgtcgcgg gggagaccga ttcgagctcg agggaccggt ggaggagctt
    11161 gcggtccccg agacgctgca ggcgctcatt gccgcacgtc tggacggtct gccaccagac
    11221 gagcgcgccc tcgtgcagga cgcctcgatc ctcgggaaaa ccttcactcg ggcgggcctc
    11281 gcggccctca cgggcaaggg cgaagacgag ctcgagcccc tgctcacgtc gctggttcgc
    11341 aaagaactgc tgagcctcca gatggatccg cgctcaccag aacgcgggca gtacgggttc
    11401 ctccagtcgc tcgtccagaa gatcgcccac gacaggctct ccaagcgcga ccggcgcgct
    11461 cgccacctcg cggccgccga cttcatcgaa cggagttggg tcggcgacga ggacgagatc
    11521 gttgaggtgg tcgcgtcgca cctgctggac gcctacgacc tggcgccgga cgccgaggac
    11581 gcgaccgccc ttcgtgaccg ggcgcgacag acgctggcac gtgccggacg gcgggcgcag
    11641 tcgctcgccg ccatgcagct cgcgcggggg tacttcgagc gcgcggcagc cttgtcggac
    11701 gatcaaatgg agcaggccgg cctgctggag caggcaggcg cctgcgcggt cccactgggc
    11761 cacatgcctg atgcggtgag gctctatgag cgcgcccggg acctctacga atccttcgga
    11821 gcggaccacg ccgcggcgcg cgcctctgcc cggatcggcg agacgctctg gctcggcgat
    11881 cgcgccgatg aggggatgag caccatggaa tctgccctcg tcgtgctcgc cgaggccgag
    11941 cccgaccacg acctcgcgct gcttcacgca gcgctcggca aggtccggtt cttcctcggc
    12001 cggtacgagg gctcggcggc cgagctcgat gccgcgatcg acatcgccga ggcattgatg
    12061 ctccccgatg tcctctctga agcgctcaac accaaggggc tcgtggccgg cgttcggggg
    12121 cgatgggaag agcagtacgc gctgctcaag cgcgcgctgg aactcgccct cgagcacgac
    12181 ctcggcgcag cggccatgcg cgcctacaac aacctgtcgt tcgcgacgat gacgcgcgat
    12241 cgctgggacg aagcccgcga gtaccaagaa gcggggatcg ccctcggcga ccgccttggc
    12301 tactcgggct ttttgtattt cctccgggcc cacctggacc agaaccgctt gcttcacggc
    12361 gagtgggaac cgttcgaggc gttgatcgac gagctgaacg ccgcaccaga cgcgacgttc
    12421 tacgcgggga cggagacgct ctcctactcg gcgagcctgg tcctgatcgc gaggggcgac
    12481 gtcgcgtccg ccagggctct gatcgatcgg ttcgtgggcg accccagcac cggtgacgtg
    12541 caggcgaggg cgttccggcg ttcggcgcga gccgcggtcc tgatcggcga gagacgctac
    12601 ggcgaggccc tcggcgaggc acgaacggtg ctggagggcg ccccggtcct gggcctcggc
    12661 cacgacgtgg tgcgattcgg gctctcgcac ggcctcgagg cggcggttcg gatcggcgat
    12721 cccgaggcag ccgaggagct catgcgcatc gtcaccgagg ctcctcccgg ggcgaccggt
    12781 ccgttcgtcc acgcccaagt cgatcgctac accgcggccg tcgccgccat gcgaggcgac
    12841 cccgccggcg ccgaatcgca cttcaaggcg gcgatcggcc tgttccgaga gctcgcgatg
    12901 cccttccacc tcgcgatggt ccagctcgag ttcggggagt ggctggtagc acagggtcgg
    12961 gccgacgagg cacgcccgac gctcgacgag gcacggagca cgttcgagga gctgaaggcg
    13021 gttccctacc tcgagcgcat cccggcggcg gcgccagacc tcgctccggc ctgacgacga
    13081 tcagcccttg ccgatgtggt ggagcgcctc ctggcgggtc cgcgcgtcat tgcggaacag
    13141 cccccggact gcagaggtca ccgtgatcga gcccggcttc ttcacgccgc gcatcgtcat
    13201 gcacaggtgc tccgcctcga tcacgacgaa gacacccctg ggctcgagcg ctcgttcaat
    13261 cgtgtcggcg atctcggtgg tgagccgctc ctgcacctgc gggcgtttgg acaggatgtc
    13321 gacgaccctc gcgactttgg acaggcctgt gattcgcccg tccttgttcg ggatgtaggc
    13381 gacgtgcgcg tggccgctga acggcagcag gtggtgctcg cacaggctct gcaacgggat
    13441 gtccttgacc atgatcatct cgtcgaagtt cgcgccgggc acgacggtcg cgaccggcgt
    13501 ggggtcgacg ccgaccccct cgaagatctc gccgtacatg tcggcgaccc gtcggggcgt
    13561 gtcgaccacg ccctcgcggt cggggtcgtc gccgatgccc tccaggatca tgcggacggc
    13621 ggcctcgatc ttcgcgcggt cgatctcggc catcaggcgc tcacca
//
LOCUS       DATBRK010000483         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_850838, whole genome shotgun sequence.
ACCESSION   DATBRK010000483 DATBRK010000000
VERSION     DATBRK010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850838"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VIH70_14035"
     CDS             complement(<1..414)
                     /locus_tag="VIH70_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY8117527.1"
                     /translation="MELATGRVVAAERHAFRHIDPILLAAAIGLAVIGLFAIYSATHQ
                     SLAAVHLDPGRFVKRQLTFLAVGAVVLMLALSFDYRLLKVYAGMIYVSSLVLLVLVRT
                     PLGTSVKGAQRWFELFGFQLAPSEIAKLALIAMLAA"
     gene            complement(414..>2096)
                     /locus_tag="VIH70_14040"
     CDS             complement(414..>2096)
                     /locus_tag="VIH70_14040"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY8117528.1"
                     /translation="LLDLSELLDIPADELGARLDDPRYYVFSPIPVATDVPKRVAYYI
                     EEHKEDFPGVDVLSEPVRRYPLGSAGAHVLGYLGQISQDKLKDPGFADYSPGDVIGVS
                     GIEGIYERYLAGTRGLVKYRVNSTGENLGLFGRQQRPVAGDDVWLTLDAGTQELAEQS
                     LKAGIEYARTIVESDSGYLRANGGAAIVLDPQTGAIEAMASYPTFDPALFTRGFSQKE
                     FDRRFGAGHSFPLVNRAIQGQYPPGSTYKPFVAASALQRGIVTTGGSYGCPPSWTAPY
                     EESDPDAVQYVFNNWTTADLGYMNLSTALARSCDTVFYPMGYRYWDMFYVNDDEAAND
                     IVSHEPLQHDLRSFGFGAQTRVDLPYEQDGRVPDAEWKQSIHKQYPESFPEGQWFPGD
                     FILMTIGQGDTLVTPLQLATAFGALENDGKECVPHVLDRVVAPDDTILRRYHQNCRKH
                     VRIDERYLRYVRDSLTGTMTGNGTAASAFAGFPLGDVWVAGKTGTAEVPPKQDYSWFA
                     AMTAAGGREHVVVVLVEQGGHGATTAAPIARHIIEGLYGLDFSQFTDVAGTD"
BASE COUNT          332 a          691 c          736 g          337 t
ORIGIN      
        1 ggccgccagc atcgcgatca gggcgagctt ggcgatctcg gagggtgcga gctggaagcc
       61 gaagagctcg aaccaccgct gtgcgccttt gacgctcgtc ccgagcggcg tccggacgag
      121 cacgagcagc acgagggagg acacgtagat catcccggcg tagaccttca ggagccggta
      181 gtcgaagctc agcgccagca tcagcacgac cgcgcccacc gcgaggaacg tgagctgacg
      241 tttcacgaac cggcccgggt ccaggtgcac ggccgcgagc gattggtggg tggccgagta
      301 gatcgcgaac agaccgatca ccgcgagccc gatcgccgcc gcgagcagga tggggtcgat
      361 gtgtcggaag gcatgccgct ccgccgcgac cacgcgcccg gtcgcgagct ccatcagtca
      421 gtccccgcaa cgtcggtgaa ttgggagaag tcgaggccgt acaggccctc gatgatgtgc
      481 cgggcgatcg gcgcggccgt cgtggcgccg tgaccgccct gctcgaccag caccaccacg
      541 acgtgctcac gcccaccggc cgcggtcatc gcggcgaacc aggagtagtc ctgcttgggc
      601 gggacctccg ccgtccccgt cttgcccgcc acccagacgt ctccgagcgg gaaaccggcg
      661 aacgcgctgg cggcggtccc gttgcccgtc atcgtgccgg tgagcgagtc ccgaacgtag
      721 cgcagatacc tctcgtcgat ccgaacgtgc ttccggcagt tctggtggta tcggcgcaga
      781 atcgtgtcgt ccggcgcgac gacgcggtcc aggacgtgcg ggacgcattc cttgccgtcg
      841 ttctcgagcg caccgaaggc ggtggcgagc tgtagcggcg tgacgagggt gtctccctgt
      901 ccgatcgtca tcaggatgaa gtcgccgggg aaccactgcc cctcggggaa cgactcgggg
      961 tactgcttgt ggatcgactg cttccactcg gcgtcgggga cccgcccgtc ctgttcgtag
     1021 gggagatcga cccgcgtctg ggcgccgaac ccgaagctcc tgaggtcgtg ctgcaatggt
     1081 tcgtgcgaga cgatgtcgtt cgcggcctcg tcgtcgttca cgtagaacat gtcccagtag
     1141 cggtacccca tcgggtagaa gaccgtgtcg caggacctcg cgagcgccgt cgagaggttc
     1201 atgtatccga ggtccgcggt ggtccagttg ttgaacacgt actgcaccgc gtcggggtcg
     1261 ctctcctcgt agggcgcggt ccagctcggc gggcagccgt aggacccacc cgtggtcacg
     1321 atgccgcgtt gcagggcgga ggccgccacg aacggcttgt aggtcgagcc cggcgggtac
     1381 tggccctgga tcgcgcgatt caccaggggg aagctgtgtc ccgcgccgaa gcggcgatcg
     1441 aactccttct gcgagaaccc gcgggtgaac agcgcggggt cgaatgtcgg gtaggaggcc
     1501 atggcctcga tcgcgccggt ctgcgggtcg agcacgatcg ccgccccgcc gttcgccctc
     1561 aggtagccgc tatcggactc cacgatggtg cgggcgtact cgatcccggc cttcagcgac
     1621 tgctccgcga gctcttgggt cccggcatcc agcgtgagcc acacgtcgtc gccggcgacg
     1681 ggccgctgct ggcggccgaa caggccgagg ttctccccgg tcgagttgac ccggtacttg
     1741 acgaggccac gcgttccggc gaggtagcgc tcgtagatgc cttcgatccc cgagacgccg
     1801 atcacgtccc ccggcgagta gtcggcgaac ccagggtcct tgagcttgtc ctgcgagatc
     1861 tggccgaggt acccgaggac gtgggcgccc gcggacccca gggggtatct gcgcaccggc
     1921 tcggagagca cgtcgacacc ggggaagtcc tccttatgct cctcgatgta gtacgcgacg
     1981 cgcttgggca cgtcggtggc gaccgggatc gggctgaaga cgtagtagcg cgggtcgtcc
     2041 agccgcgccc cgagctcgtc cgcagggatg tcgaggagct cggacaggtc gagcag
//
LOCUS       DATBRK010000484        13089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_568581, whole genome shotgun sequence.
ACCESSION   DATBRK010000484 DATBRK010000000
VERSION     DATBRK010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568581"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..2377)
                     /locus_tag="VIH70_14045"
     CDS             complement(29..2377)
                     /locus_tag="VIH70_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY8117529.1"
                     /translation="MPAAGGLTAQEVAERVALGQVNDVPAAPSRTVGQIVRANVLTRF
                     NALLGSLLVVILIVGPIQDALFGVVLVANAGVGIYQELRAKRTLDSLAVLTSPRARLV
                     RDGDVREAPLTEVVLDDVLELQPGDQVVVDGEVLEASALEVDESLLTGEAEPVIKEPG
                     DEVLSGSFVAAGGGRFRATRVGAQAYAAQLAEQGRRFGLTRSELRSGIDQILRIVTWL
                     LIPTAALLLVSQLRSDASVHDALRRSVAGTVAMVPEGLVLLTSVAFAVGVVRLAQRRV
                     LVQELPAVEVLARVDVLCIDKTGTLTEGRLVVDEVELLAEDGSHRDALAALAAAEPHP
                     NATLLAIRESFPGPSEGWLAASTFPFSSARKWSGADFGTRGTWVLGAPDVLLGSRPGD
                     DANLTKARMHAEAGRRVVLLGRTDAPIEADAGPVGLVPVAFVILTDRVRETASTTLAY
                     FARQGVDVKVISGDHPQTVAAVARRVGLPGAGAPFDAKALPAGGRELADVLETHAIFG
                     RVTPQQKRGMVEALRSRGHVVAMTGDGVNDVLALKDADIGVAMGSGSSATRAVAQLVL
                     LDSTFDALPSVVGEGRRVLGNIERTSNLYVTKTVYAMLIALGVGVVGLEFPFLPRQLT
                     LIGALTIGIPSFFLALAPNDERARPGFLSRVLRFTIPAGSLAAVATFLGYVLAKQEPG
                     VTLAEAQTAATMVLVYIGFLVLTIIAAPLTAWRLALVWAMPALFALVMAIPAAREFFA
                     LYPPPLIVWLAAFGIAALVWSFARLFLPPERPVGPGAPPAPA"
     gene            complement(2386..3795)
                     /locus_tag="VIH70_14050"
     CDS             complement(2386..3795)
                     /locus_tag="VIH70_14050"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY8117530.1"
                     /translation="MADRSRPNGGARLEIPPIATELGERFRLAGHELYLVGGVVRDLL
                     LGRELGGDLDFATDALPRETTKVLRGWTERVYLVGVRFGTVGARKADRVLEITTFREE
                     VYAEEHRKPAVTFGKDLRTDLSRRDFAVNAMAVRLPEGEFVDPFGGVKDLAARRLDTP
                     LDPEIAFSDDPLRMLRAARFAAQLELVPAPRVVRAIEQMHDRLEIVSAERINEELTKL
                     LLADAPSRGLELVVATGLAGEFLPELAALQLEQDPVHKHKDVLHHTFAVVEKTEASAD
                     PTLRLAALLHDIGKPRTRAITPEGVSFHHHEVVGARMARERLQALRYPARVVDDVARL
                     VELHLRFHGYTEWSDRAVRRYVLDAGPLLDELNQLTRADCTTQNRFKAKKLEALQDDL
                     EERIARLAEEENLDAMRPPLDGTQVMEHLGLEPGPEVGRALEHLMELRLERGPIDEDE
                     ALRQLDAWWRAERDTPGVR"
     gene            3890..4258
                     /locus_tag="VIH70_14055"
     CDS             3890..4258
                     /locus_tag="VIH70_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117531.1"
                     /translation="MRPAGWLGFFWTLPNTLLGLLLGGLTFQAPRAHGGAIVFDRVPR
                     GLTWLLRSMHRTAMTVGFVIVAAVPVEGRLLVHERHHIRQYMAWGPFFIPVYLLLAIP
                     FGYRRHPMERAARRAAGEDP"
     gene            complement(4274..5347)
                     /locus_tag="VIH70_14060"
     CDS             complement(4274..5347)
                     /locus_tag="VIH70_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY8117532.1"
                     /translation="MEITELLRHTVEVGASDLHLKAGNVPFVRVDGELSPTSFGAMSA
                     ADTEAAALALMPEHKRRAFEATSEADFGHTLTGVGRFRVNVFRQRSMVGLAIRRVRSE
                     VPTIAELMLPPVIAELADSSRGLVLVTGPTGTGKTTTIASMIGHINRTRRAHIVTIED
                     PIEVVHHDDRAIIQQREVGLDTDSYAAAMRHVVRQDPDVIFVGEIRDPESALSAIQAA
                     ETGHLVISTMHTIDSTETINRLLDLFPAQQQREVRTSFAGALRGIVSQRLVTRSDGKG
                     RVLAVEVLVSTGRVYDRIIDPEATIEIHDIIGEGGFYGMQTFDQALVGLVEGGLVTEE
                     DARFVSTSPHDFVLALRGTLTRS"
     gene            complement(5352..6050)
                     /locus_tag="VIH70_14065"
     CDS             complement(5352..6050)
                     /locus_tag="VIH70_14065"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY8117533.1"
                     /translation="MGAGPFLYWAVVDRIIDFLRPYLEPPWGYGIVFAATFLENSVGA
                     GVIVPGETLVIIGGFYARIGELWFPGVAFVAIVGAVLGDNLGYWIGRRYGRDFLERHG
                     RKLFVTPERLEAAEGYYRRHGGKTVFLGRFIPVVRSVGFIVAGVAHMEWRRFFVYDVA
                     GATIWGIGHSLLGYALGASYQRWERALTPIGIILLVVLLLLIGGSKIRAARRQVRKDL
                     EEVESGTKHEREAE"
     gene            6066..6491
                     /locus_tag="VIH70_14070"
     CDS             6066..6491
                     /locus_tag="VIH70_14070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8117534.1"
                     /translation="MGSRSEVSAGGVVYRREEEAIEIALAARRTRRGDLAWGLAKGAI
                     EPGETEEQAAVREVLEETGLEVDVESDLGDIRYFYVWDGVRVRKRVHFFLMRMTGGDV
                     EDHDTEMEDVRWFPLPSAVKRAAYQGEREVIERAVERLA"
     gene            6488..7264
                     /locus_tag="VIH70_14075"
     CDS             6488..7264
                     /locus_tag="VIH70_14075"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8117535.1"
                     /translation="MTPLQAGLGVAAGFGAGILSGLFGVGGGIVTTPAVNTILGGTAI
                     QAIATPLPVILPTSLVGSYTYARASEVSFRAARWAAVPGVVGAIVGALLTEVVNAHLL
                     LLVTAGLIGLTAVQVIAGRTPRTPWVLGETPGWKYAAVGLVAGFVSGLLGVGGGIVMV
                     PALTTWIGMPLRRALGTSLLVIAILVIPGTIVHTLLGNVDWSIFLVLTLGVIPGARIG
                     ARIALGVRERTLRAAVGTFLLVVAIAYAAAELTALAGGRG"
     gene            7266..9368
                     /locus_tag="VIH70_14080"
     CDS             7266..9368
                     /locus_tag="VIH70_14080"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEY8117536.1"
                     /translation="MARPIMRRLPPLVRPLTSVLVFVLLVVPGWPAPPATAQESPSVL
                     LTLLSQTSWNCPNATEDLAPGPSAWSCPAGRDLVVRFRAQNLGATPLDELAIGVTLYS
                     RVLSRSEYEGSLESDPPIVIDGGTLPREGAIPPGRTRDFEVPLTLADGIDPDQSGVYP
                     LKLDLRSGVESVAALRTPVIFLVREPEIPLGLSWTLVMSHPIAFAPDGTFNDPSLELA
                     LGPGGRLNGEIRSLLELVSDPAAPAVDVAIAPVLLTQLGRMADGYKVAEGGEVRQVPP
                     AEGGAALARQALEHLRTIAAAPNIEVTALPFSAPEIPSLYGGGLGRDVEVQVQHGRDV
                     ISTLIGAEIPQTILRPPGAVLDDPTLRGLAAEGIRTLIVGPTTVDLPEQPLGFAGPPT
                     TSLGDGALAAVVPEPASDALLGSVIEQDPVRAAQVLLGELATIWQEQPGETRGVALVL
                     GEDAPLPGPFFPPIVRGVATAPWLEPTVVGDFVATFPPTHESVLASPSLRRFPTTYVA
                     SLKQARRRIDTLRSMLPDSSLEPARLETMLLLAEARQYLTSPTGGLAFIDAARRSVRA
                     TVDDLALETVDSVTLTSDSEGIPVTVSNEGPHSLTFLVQLVSTRIREEPATLVKLGPD
                     ESETLRLRASLRSTGRFEVHVLMVSPSGRLIGQETFVVRSTAYNRIALLITIGAALVL
                     LLVWARRFVPGRTSPERT"
     gene            9376..11073
                     /gene="murJ"
                     /locus_tag="VIH70_14085"
     CDS             9376..11073
                     /gene="murJ"
                     /locus_tag="VIH70_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY8117537.1"
                     /translation="MADDLTEGLTPGGSEPPSRPPPTARAEPPPEDSTPSFVRNTIVM
                     STGTAISRLTGFLRLSAMAYAIGITETRLADAYNVANITPNILYELALGGILTSVVVP
                     VVVEWMQARGRDLAWDVVRRLLTIAIVVLSAIAILGIVLAPWIVDVYTVGYPDAQREA
                     VHGLATFFLRWFMPQIVFYGIGAVAAGLLNAHRRFAAPMFAPIANNIIVIATFLTFAA
                     IAPENPTELATDAQRLVLAIGTTLGVVAMTAALWPSVRATGFRFRWIGGVRDEAIGRI
                     GRLATWVVVYVVANQLGYLVIVILAGGPTGGYSSYAAAFILFQLPHAIFAVSILTALL
                     PAMSSRWAADDLDGYKALLSQGIRATATILIPAALGYLVLAKPIVRLLLEHGETEAAS
                     AELVSEVLFAFALGLFAFSAFQFLLRASYAMQDTRTPALVNVAAVAVNVLVDLLLFFG
                     LELGIPGLALGHAVSYAFASSVLLLLIRRRIGPIGGRRILASLGRILVAGLATAAVAW
                     LAAEGFERWLGTTTLAAQTVQVLGAVVAGLAVFIASAAALRIEEVDLVRRQVAARWR"
     gene            11070..11498
                     /locus_tag="VIH70_14090"
     CDS             11070..11498
                     /locus_tag="VIH70_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117538.1"
                     /translation="MTMPGPSRGDPPRHAPLRDERGLIGKILLVWLLLAAVIVVGAID
                     AGSILLTRSRAGDLAKDASVSAADAFRRSGDEEQAKLAALDTIADADEPVRLKRIGVG
                     RRAVTVVLVVRADTLVLGRIPFLDDLERVTVSGSTPAPRD"
     gene            11552..12967
                     /locus_tag="VIH70_14095"
     CDS             11552..12967
                     /locus_tag="VIH70_14095"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8117539.1"
                     /translation="MTTSIEPAHVLAGRYALEEELGRSRTGMVWRSQDPLLRRSVAVK
                     LIHPRLAEDEGFARALALEARRVAGLSERGIACLLDTGEQDGVIYLVREYAPGSSLRD
                     RLDTAGPATPSEAVRIGVAALRALAEAHDRGVLHLALDLDDVIVGDDDRVCLIDFGIG
                     AAVASSRPGEATTLLGDEYLAPEQAAGAEPDARTDVFAMASLLFELLTSEPPRGRTSA
                     RTVRPFLPRALDRVLARALDPDPERRFPDARAFADALARSVETEPVDASDEGRASLFT
                     WIGVPLVVAGIAIVTIALGLWMGTLEVGGPLGIRAAPDEPAAVETPPELVRPISVGAI
                     DPFGDGDEHSANAGLADDGDLQTAWRSENYYDGELGKAGFGLLLDLGERHEVLGLRLW
                     TPHPGFRFRLAVGDDPDTLADEAGGEVTSTSLSRVSLRGAGRFVLVWITSVVPVDDGI
                     RAEVAEVRVVVRNTDAETVGA"
BASE COUNT         1783 a         4772 c         4617 g         1917 t
ORIGIN      
        1 ccgcaccgaa ccgccgcggc gcgaacgctc aggcgggcgc cggcggagcg ccgggaccca
       61 cgggccgctc cggcggcagg aacagccgcg cgaacgacca caccagcgcc gcgatgccga
      121 acgccgccag ccacacgatc agcggcgggg ggtacagcgc gaagaactcc cgggccgccg
      181 ggatcgccat cacgagcgcg aacagcgcgg gcatcgccca caccagcgcc agccgccacg
      241 ccgtgagcgg cgcggcgatg atcgtgagca ccaggaaccc gatgtagacc agcaccatcg
      301 tcgcggccgt ctgggcctcg gcgagcgtga cccccggctc ctgcttcgcc agcacgtaac
      361 caaggaacgt cgcgaccgcc gcgagcgagc ccgcggggat cgtgaaccgc aggacacgcg
      421 agaggaaccc cggacgcgcg cgctcgtcgt tcggcgccag cgcgaggaag aacgacggga
      481 tcccgatcgt gagggcgccg atcagcgtca gctgtcgcgg caggaacggg aactcgagcc
      541 ccaccacgcc gacccccagc gcgatcagca tcgcgtacac ggtcttggtc acgtacaggt
      601 tcgaggtgcg ttcgatgttg ccgagcacgc gacgaccctc gcccaccacc gaggggaggg
      661 cgtcgaacgt cgagtccagc agcacgagct gcgcgaccgc gcgcgtcgcg gagctgcccg
      721 aacccatggc gaccccgatg tcggcgtcct tcagcgcgag cacgtcgttc accccgtcgc
      781 cggtcatcgc gacgacgtgc cctcgcgatc ggagcgcctc gaccatcccc cgcttctgct
      841 gcggggtgac ccttccgaag atcgcgtgcg tctcgagcac gtcggcgagc tcccgaccgc
      901 ccgcggggag cgccttcgcg tcgaacgggg ccccggcgcc gggcagaccg actcgccggg
      961 cgaccgcggc cacggtctgg gggtggtccc cgctgatcac cttgacgtcc acgccctgcc
     1021 gggcgaagta cgcgagcgtc gtcgaggccg tctcgcgaac acggtcggtc aggatcacga
     1081 aggcgaccgg gacgagcccg accggcccgg cgtccgcctc gatcggggcg tcggtccggc
     1141 cgaggagcac cacccgccgt cccgcctcgg cgtgcatgcg cgccttcgtg aggttggcgt
     1201 cgtcgcccgg ccgcgagccg agcagcacgt ccggcgcgcc gagcacccag gtccccctgg
     1261 tcccgaagtc ggcgccgctc cacttgcggg cggaggagaa cggaaaggtc gaggcagcga
     1321 gccagccctc ggagggtccg gggaagctct cgcggatcgc gagcagggtg gcgttggggt
     1381 ggggttcggc ggccgcgagc gcggcgagcg cgtcgcggtg cgacccgtcc tccgccagca
     1441 gctcgacctc gtcgacgacg aggcggccct cggtgagcgt gccggtcttg tcgatgcaca
     1501 ggacgtcgac ccgggcgagc acctcgaccg ccggcagctc ctggacgagc acgcggcgtt
     1561 gggcgaggcg gacgacgccg accgcgaacg ccacgctggt gagcagcacc agaccctcgg
     1621 ggaccatcgc gaccgtcccg gcgaccgagc gccgcagcgc gtcgtgcacg ctcgcgtcgg
     1681 agcggagctg gctgaccagg agcagcgcag cggtcgggat cagcaaccac gtcacgatcc
     1741 gcaggatctg atcgatcccg ctccgaagct cggaacgcgt caggccgaac cgccgcccct
     1801 gctcggcgag ctgcgccgcg taggcctggg ccccgacgcg cgtggctcgg aaccgaccgc
     1861 cgcccgccgc gacgaacgac cccgagagca cctcgtcccc cggctccttg atcacgggct
     1921 cggcctcgcc ggtcaggagg gactcgtcga cctcgagcgc ggaggcctcg agcacctcgc
     1981 cgtcgacgac gacctgatcg cccggttgca gctcgagcac gtcgtccagg acgacctcgg
     2041 tgagcggcgc ctcgcgaacg tcgccgtcgc ggaccagacg cgcgcgcggc gacgtcagca
     2101 ccgccagcga gtccagcgtc cgcttggcgc gcagctcctg gtagatcccg acaccggcgt
     2161 tcgcgaccag cacgaccccg aacagcgcgt cctggatcgg gccgacgatc aggatcacga
     2221 ccaggagcga gccgagcagc gcgttgaacc tcgtgaggac gttggctcgg acgatctgcc
     2281 cgacggtcct cgacggggct gccggaacgt cgttgacctg gccgagcgcg acgcgctcgg
     2341 cgacctcctg cgcggtcagg cccccggcgg cgggcacggt tgccgctatc ggacgcccgg
     2401 cgtatcgcgc tcggcccgcc accacgcgtc gagttggcgg agggcctcgt cctcgtcgat
     2461 cgggccgcgt tcgagacgga gctccatcag gtgctcgagc gcccgtccga cctcgggacc
     2521 cggttcgagc ccgaggtgtt ccatcacctg cgtgccgtcc agcggcggac gcatcgcgtc
     2581 gaggttctcc tcctcggcca gccgtgcgat ccgctcctcg aggtcgtcct ggagcgcctc
     2641 gagcttcttg gccttgaacc ggttctgcgt cgtgcagtcg gcgcgcgtga gctggttcag
     2701 ctcgtcgagc agcggccccg cgtccagaac gtaccggcgg accgccctgt cgctccactc
     2761 ggtgtagccg tggaagcgca gatgcagctc gacgagacgg gcgacgtcgt ccacgacgcg
     2821 cgccgggtac cgcagcgcct ggaggcgttc gcgcgccatg cgcgccccga cgacctcgtg
     2881 atggtggaac gacacgccct cgggcgtgat cgcgcgggtc ctcggcttgc cgatgtcgtg
     2941 gagcagcgcc gcgagccgga gcgtcggatc ggcgctcgcc tcggtcttct cgacgacggc
     3001 gaacgtgtga tgcagcacgt ccttgtgctt gtgcacgggg tcctgctcga gctgcagcgc
     3061 cgccagctca ggcaggaact ccccggccag gccggtcgcg accaccagct cgagcccgcg
     3121 cgagggcgcg tcggccagga gcagcttcgt gagctcctcg ttgatccgct ccgcggacac
     3181 gatctccagg cggtcgtgca tctgctcgat cgcccgcacc acgcgcggag ccgggacgag
     3241 ctcgagttgc gcggcgaacc tcgcggctcg gagcatgcgc agcgggtcgt cggagaacgc
     3301 gatctcggga tcgagcggag tgtccagccg tctagccgcg aggtccttca ctccgccgaa
     3361 cggatcgacg aactccccct cgggcagacg gaccgccatc gcgttcacgg cgaagtcgcg
     3421 tcgcgacagg tcggtccgca ggtccttgcc gaacgtgacc gccggctttc ggtgctcctc
     3481 cgcgtacacc tcctcgcgga acgtcgtgat ctcgagcacc cgatccgcct tgcgagcacc
     3541 caccgtgccg aacctcacgc cgaccaggta cacgcgctcg gtccacccgc gcaggacctt
     3601 cgtggtctcg cgcgggaggg catcggtcgc gaagtcgaga tcgcccccga gctcccggcc
     3661 cagcagcaga tcccggacca cgccgcccac gaggtagagc tcgtgaccgg ccaggcggaa
     3721 gcgctcgccg agctcggtgg cgatcggggg gatctcgagg cgcgcgccgc cgttcggacg
     3781 cgaccggtcg gccatgacgc gctcaggata cccctgcacc cgagcggccc cgcttcaccc
     3841 gagcggctcc cgctccgagc cggcggcggc ctgcggtacc gtccgaggcg tgcgccccgc
     3901 cggatggctc ggcttcttct ggacactgcc gaacacgctc ctcggactcc tgctcggcgg
     3961 gctcacgttc caggcgccgc gcgcgcacgg cggcgcgatc gtgttcgacc gcgttccgcg
     4021 gggtctgact tggctcctgc gctcgatgca tcgcaccgcg atgaccgtcg gcttcgtgat
     4081 cgtcgcggcg gttccggtcg agggacggct cctggtccac gaacgccacc acatccgcca
     4141 gtacatggcg tgggggccgt tcttcatccc ggtctacctg ctgctcgcga tcccgttcgg
     4201 ctaccgccgg caccccatgg agcgcgcggc tcgccgagcc gcgggcgagg acccgtgaaa
     4261 cgtgaggacg cggctagctc cgggtgagcg tcccgcggag cgcgagcacg aagtcgtgcg
     4321 ggctcgtcga gacgaaccgc gcatcctcct ccgtcaccag accgccctcg acgagaccca
     4381 cgagcgcctg gtcgaacgtc tgcatgccgt agaagccacc ctcgccgatg atgtcgtgga
     4441 tctcgatcgt cgcctccggg tcgatgatcc gatcgtagac acggccggtc gagacgagca
     4501 cctcgaccgc caggacgcgg cccttgccgt cggagcgcgt cacgaggcgt tgcgacacga
     4561 tcccgcgcag cgcgcccgcg aacgacgtgc ggacctcccg ctgctgctgg gcggggaaca
     4621 ggtcgagcag ccggttgatc gtctcggtcg agtcgatcgt gtgcatcgtc gagatcacga
     4681 ggtgaccggt ctccgccgcc tggatcgccg acagggcgga ctccgggtcg cggatctcgc
     4741 cgacgaagat cacgtcgggg tcctgccgca cgacgtgccg catggccgcc gcgtacgaat
     4801 ccgtgtcgag cccgacctcg cgctgctgga tgatcgcccg gtcgtcgtgg tggacgacct
     4861 cgatggggtc ctcgatcgtc acgatgtgcg cgcgccgcgt gcggttgatg tggccgatca
     4921 tcgacgcgat cgtcgtcgtc ttgccggtgc cggtcggccc cgtcaccagc accagcccgc
     4981 ggctcgagtc cgcgagctcg gcgatgacgg gggggagcat cagttcggcg atggtcggga
     5041 cctccgagcg gacccgccgg atcgccagcc cgaccatcga ccgctgccgg aagacgttga
     5101 cccggaaccg accgacgccc gtcagcgtgt ggccgaagtc ggcctcgctg gtggcctcga
     5161 acgcacgccg cttgtgctcc ggcatcagcg cgagggcggc ggcctccgtg tcggcggccg
     5221 acatggcccc gaacgacgtc gggctgagct cgccgtcgac gcgcacgaac ggcacgttcc
     5281 cggccttcag atggaggtcg cttgccccca cctcgaccgt gtgccggagc aactcggtga
     5341 tctccacgtg cctactcggc ctcccgctcg tgcttggtcc cggactcgac ctcctcgagg
     5401 tccttcctca cttgtcggcg cgcggcccgg atctttgagc ccccgatcag aagaagcagg
     5461 accacgagca ggatgatccc gatcggggtg agggcgcgct cccagcgctg gtagctggct
     5521 ccgagcgcgt agccgagcag ggaatggccg atcccccaga tcgtcgcccc ggcgacgtcg
     5581 tagacgaaga accgacgcca ctccatatgc gccacgccgg ccacgatgaa cccgacgctg
     5641 cgtacgaccg ggatgaaccg accgaggaac acggtctttc ctccgtgccg gcggtagtag
     5701 ccctcggcgg cctcgagccg ttcgggcgtg acgaacagct tgcgcccgtg gcgctcgagg
     5761 aagtcccggc cgtagcgccg cccgatccag tacccgaggt tgtcccccag caccgcgccg
     5821 acgatcgcga cgaaggcgac gccggggaac cagagttcgc cgatgcgcgc atagaatccg
     5881 ccgatgatga cgagggtctc gcccgggacg atgacgccgg cgccgaccga gttctcgagg
     5941 aatgtcgccg cgaacacgat gccgtatccc cacggaggct cgaggtacgg tcggaggaag
     6001 tcgatgatcc ggtcgaccac ggcccagtat aggaaggggc ccgctcccac cggggcatgc
     6061 ctctgatggg atcccggagc gaggtgtccg ccggcggcgt cgtctatcga cgcgaggagg
     6121 aggcgatcga gatcgcgctc gccgcgcgcc ggacgcggcg cggcgacctc gcgtggggac
     6181 tcgcgaaggg cgcgatcgag cccggcgaga ccgaggaaca ggccgcggtg cgcgaggtcc
     6241 tcgaggagac cgggctcgag gtcgacgtgg agtccgacct gggcgacatc cggtacttct
     6301 acgtgtggga cggtgtccgc gtccgcaagc gggtgcactt cttcctgatg cggatgaccg
     6361 gcggtgacgt cgaggatcac gacaccgaga tggaggacgt ccggtggttc ccgctgcctt
     6421 cggccgtcaa gcgggccgcg taccaaggcg aacgcgaggt catcgagcgc gccgtcgaac
     6481 ggctcgcgtg acgccgctgc aggccgggct cggcgtcgcc gcggggttcg gtgccgggat
     6541 cctgtcgggt ttgttcggcg tcggcggcgg gatcgtcacg acccccgcgg tgaacacgat
     6601 ccttggcggg accgcgatcc aggcgatcgc cacgcccctg cccgtcatcc tgccgacctc
     6661 gctcgtgggt tcgtacacct acgcgagggc gagcgaggtg agcttccggg ctgcccgatg
     6721 ggcggccgtc cccggggtcg tcggcgcgat cgtcggcgcg ctgctgaccg aggtcgtgaa
     6781 cgcgcacctg ctgctcctgg tcaccgcggg gctgatcggg ctcacggccg tccaggtgat
     6841 agccgggcgc actccccgga cgccgtgggt cctgggcgag acgccgggat ggaagtacgc
     6901 cgcggtcggc ctggtcgcgg ggttcgtctc gggtctgcta ggcgtgggtg gcgggatcgt
     6961 gatggtgccc gccctcacga cttggatcgg catgccgctt cggcgggcgc tcgggacctc
     7021 gctgctggtg atcgcgatcc tggtgatccc cgggaccatc gtccacacgc ttctcggcaa
     7081 cgtcgactgg tcgatcttcc tggtgctcac cctcggcgtg atccccggag ccaggatcgg
     7141 agcgcgcatc gcgctcggcg tgcgcgaacg caccctgcgc gccgccgtcg gtacgttcct
     7201 gctggtcgtg gcgatcgcgt acgcggccgc ggagctcacc gccctggccg gggggcgagg
     7261 gtaggatggc gcgaccgatc atgcgacggc tcccgccgct cgtccgcccg ctcacgtccg
     7321 tgctcgtgtt cgtcctcctg gtcgtccccg gttggccggc gccccccgcg acggcgcagg
     7381 agagcccctc ggtcctgctc acgctcctgt cgcagacgtc gtggaactgc ccgaacgcga
     7441 ccgaggacct cgcgcccggc ccgtcggcct ggagctgccc ggccggtcgc gacctcgtcg
     7501 tgcggttccg cgcgcagaat ctcggggcga ccccgctcga cgagctcgcg atcggggtca
     7561 cgttgtactc gcgggtcctg tccaggagcg agtacgaggg ctcgctcgag tccgacccgc
     7621 cgatcgtgat cgacggtggg accctgccga gggagggggc gatcccgccc ggcaggacga
     7681 gggacttcga ggtgccgctg acgctcgccg atgggatcga ccccgaccaa tcgggcgtct
     7741 acccgctcaa gctcgacctc cgcagcggcg tcgagtcggt cgcggccctg cggacgcccg
     7801 tgatcttcct cgttcgcgaa cccgagatcc cgctcgggct gtcgtggacc ctcgtcatgt
     7861 cgcacccgat cgcgttcgcg ccggacggca cgttcaacga cccctccctc gagctcgcgc
     7921 tcggccccgg cgggcggctg aacggcgaga tccgctcgct gctggagctc gtgtcggatc
     7981 ccgcggcccc ggcggtcgac gtggcgatcg ccccggtcct gctgacccag ctcggacgga
     8041 tggccgacgg ctataaggtc gccgaagggg gcgaggtccg gcaggtcccg ccggccgagg
     8101 gcggcgccgc cctggcccgg caggcgctgg agcacctccg cacgatcgcc gccgccccga
     8161 atatcgaggt caccgcgctc cccttctcgg cacccgagat cccgtcgctc tacggcggcg
     8221 gtctcggacg agacgtcgag gtccaggtcc aacacggccg ggacgtgatc tcgacgctga
     8281 tcggggcgga gatcccccag acgatcctgc gaccgcctgg cgccgtcctg gacgacccga
     8341 cgctgcgggg cctggccgcc gaaggcatcc gaacgctgat cgtcggtccc accaccgtcg
     8401 acctgccaga gcaaccgctc ggcttcgcgg gtccgccgac cacgtcgctg ggcgacggcg
     8461 cgctcgcggc cgtcgtcccc gaaccggcga gcgacgccct cctcggatcg gtgatcgaac
     8521 aggacccggt ccgcgccgcc caggtcctgc taggcgagct cgcgacgatc tggcaggagc
     8581 agccgggcga gacgcgcggc gtcgcgctgg tcctcggcga ggacgcgccg ctccccggtc
     8641 ccttcttccc accgatcgtc cggggcgtcg cgaccgcgcc ctggctcgag cccaccgtcg
     8701 tgggcgactt cgtcgcgacg ttcccgccga cccacgagtc ggtcctcgcg tcgccgtcgc
     8761 tccgacggtt cccgaccacg tacgtcgcct cgctcaagca ggcgaggagg cgcatcgaca
     8821 cgctccgctc gatgctcccc gacagcagcc tcgaacccgc tcgcctcgaa acgatgttgc
     8881 tgctggccga ggcgcgccag tacctgacct cgccgaccgg aggacttgcg ttcatcgacg
     8941 cggcccgccg gagcgtgcgg gcgaccgtgg acgatctcgc gctcgagacg gtggactcgg
     9001 tgacgctcac gtcggactcc gaggggatcc cggtgacggt ctcgaacgag ggaccgcact
     9061 cgctcacgtt cttggtgcag ctcgtctcga ccaggatccg ggaggaaccg gcgacgctcg
     9121 tcaaactcgg gcccgacgaa tccgagaccc tccgcctccg ggccagtctg cgttcgacgg
     9181 gacggttcga ggtgcacgtg ctgatggtct cgccgagcgg gcgcctgatc ggccaggaga
     9241 ccttcgtcgt gcgttcgacg gcctacaacc gcatcgcgct gctgatcacg atcggtgccg
     9301 cgctcgtgct cctgctcgtg tgggcgcggc ggttcgtgcc ggggcggaca tcacccgaaa
     9361 ggacctgacg atcggatggc cgatgacctg acggagggcc tgacgccggg cgggagcgaa
     9421 cccccgtcac gcccaccgcc gacggcccgg gccgaaccgc cgcccgagga ctcgacgccc
     9481 agcttcgtcc gcaacacgat cgtgatgtcc accggcaccg cgatctcacg gctgaccggc
     9541 ttcctccgcc tgtcggcgat ggcctacgcg atcggcatca ccgagacgcg cctggccgac
     9601 gcctacaacg tcgcgaacat cacgccgaac atcctgtacg agctcgcgct cggcggaatc
     9661 ctgacctccg tcgtcgtccc cgtcgtcgtc gagtggatgc aggcgcgcgg tcgcgacctg
     9721 gcgtgggacg tggttcggcg cctgctcacg atcgcgatcg tcgtgctctc ggcgatcgcg
     9781 atcctcggca tcgtgctggc gccgtggatc gtggacgtgt acacggtcgg gtaccccgac
     9841 gcccagcgcg aggcggtgca cggcctcgcc acgttcttcc ttcgctggtt catgccgcag
     9901 atcgtcttct atgggatcgg cgccgtcgcc gccgggctgc tgaacgctca tcggcggttc
     9961 gccgcgccga tgttcgcccc gatcgcgaac aacatcatcg tcatcgcgac gttcctgacg
    10021 ttcgccgcga tcgcccccga gaaccctacg gaactcgcga cagatgcgca gcggctcgtc
    10081 ctcgcgatcg gaacgacgct cggcgtggtc gcgatgaccg cggcgctgtg gccctcggtg
    10141 cgcgcgacgg ggttccggtt ccgatggatc ggcggcgtcc gcgacgaggc gatcggtcgg
    10201 atcggtcgcc tcgcgacgtg ggtggtcgtc tacgtggtcg cgaaccagct cggctacctc
    10261 gtgatcgtga tcctcgccgg cggcccgacc ggtggttact cctcgtacgc cgcggcgttc
    10321 atcctgttcc agctccccca cgcgatcttc gcggtgtcga tcctgaccgc gctgctgccg
    10381 gcgatgtcct cgcggtgggc ggccgacgac ctcgatggct acaaggccct gctctcgcag
    10441 gggatccggg cgacggccac gatcctgatc ccggcggcgc tcggctacct ggtgctcgcg
    10501 aaaccgatcg tccgactcct gctcgagcac ggcgagaccg aggccgccag cgccgagctc
    10561 gtctccgagg tgctgttcgc cttcgcgctc gggctgttcg cgttctcggc gttccagttc
    10621 ctgctccggg cgtcgtacgc gatgcaggac acgcggacgc ccgcgctcgt gaacgtcgcc
    10681 gcggtcgcag tgaacgtgct cgtcgatctg cttctcttct tcgggctgga gctcgggatc
    10741 cccgggctcg cgctcggaca cgccgtctcg tacgccttcg cctcgagcgt gctgctgctt
    10801 ctgatccgcc ggcgcatcgg cccgatcggc gggcgtcgga tcctcgcgtc gctcggccgg
    10861 atcctcgtgg cggggctcgc gaccgcggcg gtggcctggc tcgcggccga ggggttcgag
    10921 cggtggctcg ggacgacgac gctcgccgcg cagaccgtgc aggtgctcgg agcagtggtg
    10981 gcggggctcg ccgtgttcat cgcgtccgcc gcggcgctgc ggatcgagga ggtcgacctg
    11041 gtgcggcggc aggtggccgc gcgctggcga tgacgatgcc gggaccctcg cgcggcgatc
    11101 cgccccggca cgctccgctc cgagacgaac gggggctgat cggcaagatc ctgctcgtgt
    11161 ggctgctgct tgcggcggtg atcgtggtcg gcgcgatcga cgcgggttcg atcctgctga
    11221 cccggtcgcg cgcgggcgac ctggcgaagg acgcctcggt ctcggcggcc gacgccttcc
    11281 ggcgcagcgg cgacgaggag caggccaagc tcgcggccct ggacacgatc gccgacgccg
    11341 acgagcccgt gcggctcaag cgaatcggcg tcgggcgccg ggcggtgacc gtggtgctcg
    11401 tcgtccgggc cgacacgctc gtcctggggc ggatcccctt cctcgacgac ctcgagcgcg
    11461 tgacggtcag cggctcgacc ccggcgccgc gcgactgagc aaaactccct cgcacggctg
    11521 ggagcccttc ccccgggcat agactcgaac agtgaccacg tcgatcgagc ccgcccacgt
    11581 cctcgccggc cgctacgctc tcgaggagga gctcggcagg agccggaccg ggatggtgtg
    11641 gcggtcgcag gacccgttgc tgcgacgctc ggtggccgtg aagctgatcc accctcggct
    11701 cgcggaggac gaggggttcg cccgggcgct cgccctggag gcccggcgcg tggccgggct
    11761 gtcggagcgc gggatcgcct gcctgctcga caccggcgag caggacggcg tgatctacct
    11821 cgtccgcgag tacgcgccgg ggtcgagcct gcgcgacagg ctggacactg ccggccccgc
    11881 gacgccgtcc gaggccgttc ggatcggggt cgccgccctg cgggcgctcg ccgaggcgca
    11941 cgatcgcggc gtgctccacc tcgcgctcga cctcgacgac gtgatcgtcg gcgacgacga
    12001 ccgggtgtgc ctgatcgact tcgggatcgg tgccgccgtc gcgtcgagca ggccgggcga
    12061 ggccacgacc ttgctcggcg acgagtacct cgcccccgag caggccgccg gcgccgaacc
    12121 cgatgcacgc acggacgtct tcgccatggc atcgctcctg ttcgagctcc tcacgagcga
    12181 accgccccgc ggtcgcacct ccgcccggac cgtccgtcct tttctcccac gcgcgctcga
    12241 ccgcgtcctg gctcgagcgc tcgatcccga tcccgagcgc aggttcccgg acgcgcgtgc
    12301 gttcgccgac gcgctcgccc gctcggtgga gaccgagccc gtcgacgcgt ccgacgaggg
    12361 gcgggcgagc ctgttcacct ggatcggggt gccgctcgtc gtggcgggga tcgcgatcgt
    12421 cacgatcgcg ctggggctat ggatgggcac gctcgaggtg ggcggtcccc tcgggatccg
    12481 cgccgcaccc gacgagcccg cggcggtcga gaccccgccc gagctggtcc ggccgatctc
    12541 cgtgggcgcg atcgacccgt tcggcgacgg cgacgagcac tcggcgaacg cgggtctggc
    12601 cgacgacggg gacctgcaga cggcgtggcg atcggagaac tactacgacg gcgagctcgg
    12661 gaaggccggg ttcgggctcc tgctcgacct cggcgagcga cacgaggtgc tggggttgcg
    12721 gttgtggacg ccgcatcccg ggttccggtt ccgcctcgcg gtcggcgacg accccgacac
    12781 gctcgcggac gaggccgggg gggaggtcac ctcgacctcc ctgtcccgcg tctcgctgcg
    12841 gggcgcgggc cggtttgtgc tcgtctggat cacgtcggtc gtgccggtcg acgacggcat
    12901 ccgcgcggag gtggccgagg tgcgggtggt cgtccggaac accgacgccg agacggtcgg
    12961 tgcctgagcc gccggcccgc gacgaagacc tcgtccgccg gtacctgtcg ggagatcgag
    13021 ccgccttcgc cgcgctggtc gaacgccacg agcggcgggt ctacaacctg gccctgcgca
    13081 tgacgggac
//
LOCUS       DATBRK010000485         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_2885381, whole genome shotgun sequence.
ACCESSION   DATBRK010000485 DATBRK010000000
VERSION     DATBRK010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2885381"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            672..1613
                     /locus_tag="VIH70_14100"
     CDS             672..1613
                     /locus_tag="VIH70_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6454 family protein"
                     /protein_id="HEY8117540.1"
                     /translation="MKVRTAVAAALVAALIVVVALVSGAAGAPRDDALVDAIEHASRA
                     TPWEVVRRVPLGFQTYHPQGFARVGDRLYMSSVEIIEAPQKYPQPVGGYDRTPGKGIA
                     HLFELSLDGRLLAQTTLGEGIIYHPGGIDYDGQSLWVPVAEYRPNSRSIVYRVDPDTL
                     RAEEVFRVNDHVGGIVRDEVSGELHGVSWGSRTFYAWTSGGKELDRTPNPEQYVDFQD
                     CQYARFRKAVCTGITEYTGPTGAKFELGGIDVVNLERGTIGAEVPVQRYSAAGHVVTR
                     NPVDLEVAGGKLRMYAAPDDYGEPANTELLTLEAPLA"
BASE COUNT          253 a          562 c          571 g          238 t
ORIGIN      
        1 taaaagatca ccggagggaa ggggcagctg gttggctggt cccgggtgcg aggtcttgtc
       61 ggtgccgggg catgcgacca tgctcatcgc gggcaggcag cagcatcggt gctcgccggc
      121 ccgcgggcga tcggagggag ccattggaag cagcaagcga ccagtcccgg cggggacgcg
      181 tacctgtacg ccctgcggga cgaacaggca tacctcgaga ccgaggatga gaagccggcg
      241 atcgtcgcgc gacccccgcc cgaccctgcc cagttcaagt gctggttctg ccggaagtcc
      301 caccagcagg tgcaggtgct gtttggcgcc gagtgcccgg tcagggaccc caaacgactt
      361 cgcttgcgag acgctgatct ttgtctgcaa cgagtgcgtc gccaagttcg cggagcggct
      421 ggccaagggc gtcccgggag ctccaccgta gctgccgcct cgcaacgcgc ggtaggtcgg
      481 cgggcggggc gacgctccac tgcaaggtcc tcccgagtcc acacaaccgg tggtgtgccc
      541 gtctcgcacc tggcgtccaa caccaccatc tacggctgag caccgcagta cccaaggtgt
      601 caccgcaagg ggcgagcttc tcgtagctgc gatggcggct cgccagggtt cgcgggaggg
      661 gaggttcctc catgaaggtc cgcactgccg ttgcggcagc gctggtcgct gccctcatcg
      721 tcgttgtcgc gctggtatca ggcgcggccg gtgcgccgcg cgacgacgcg ctcgtcgatg
      781 ccatcgagca cgcgtcgcgc gccacgccct gggaggtcgt ccggcgcgtt ccgctcggct
      841 tccagaccta ccacccccag ggcttcgcgc gcgtgggtga ccgcctgtac atgtcgagcg
      901 tcgagatcat cgaggcgccg cagaagtacc cgcagccggt cggcggctac gaccgcacgc
      961 ccggcaaggg gatcgcgcac ctgttcgagc tctcgctcga cgggcggctg ctcgcgcaga
     1021 cgacgctcgg cgaggggatc atctaccacc ccggcggtat cgactacgac ggccagtcgc
     1081 tctgggtgcc ggtcgccgag taccggccca acagccggtc gatcgtctac cgcgtggacc
     1141 cggacaccct gcgcgccgag gaggtgttcc gcgtgaacga ccacgtcggc ggcatcgtcc
     1201 gggacgaggt cagcggcgag ctccacggcg tgtcctgggg ttcccgcacg ttctacgcct
     1261 ggacgtccgg cggcaaggag ctggaccgca cgccgaaccc cgagcagtac gtggacttcc
     1321 aggactgcca gtacgcccgc ttccgcaagg cggtgtgcac cgggattacc gagtacaccg
     1381 ggccgacggg ggcgaagttc gagctcggcg gcatcgacgt cgtcaacctc gagcgcggca
     1441 cgatcggggc cgaggtgccc gtgcagcgct actcggccgc cgggcacgtc gtgacccgca
     1501 acccggtcga cctcgaggtc gcgggtggga agctgcgcat gtacgcggca cccgacgact
     1561 acggcgagcc cgcgaacacc gagctgctca cgctcgaggc gccgctggcc tgacgccgct
     1621 gcgc
//
LOCUS       DATBRK010000486        10697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1145925, whole genome shotgun sequence.
ACCESSION   DATBRK010000486 DATBRK010000000
VERSION     DATBRK010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10697
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145925"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..918
                     /locus_tag="VIH70_14105"
     CDS             271..918
                     /locus_tag="VIH70_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117541.1"
                     /translation="MGDEHWYRGAAWDKVARAEFEERLSRARSQRPQYMRIKATHLLD
                     SDDPIARAGGVDLMRRLIDEYPDDRLSVPFAHEQLAMYFEKQGLREEAKNEYRQSIAK
                     ALPNRSGTRWPDLSLVELLIDDGEVEEALERWEIARTMSRGQDIFPAVAFDFHRIEAK
                     VSLTIGDQQAAEAAAQKALAAAAATQSNALHHRNLGLVESDEATLAFLRRTARRA"
     gene            complement(2218..2562)
                     /locus_tag="VIH70_14110"
     CDS             complement(2218..2562)
                     /locus_tag="VIH70_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117542.1"
                     /translation="MILYKNLTSLFKKYYPHIKLASELEKELAIADLVGSYNFVKTHA
                     AIRQLEAYPDFSAEQSRDLINAAISNDQIYRIYDDEDVYEFFTQLAIDHQDDIDPEEW
                     EHFWAMFSQAED"
     gene            complement(2559..2786)
                     /locus_tag="VIH70_14115"
     CDS             complement(2559..2786)
                     /locus_tag="VIH70_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117543.1"
                     /translation="MNRADALVDVRRTLHERLIGRVDARQLDAMPRAERRLRVREEAL
                     SLLREQGHMLAQSALAEARSVKNGPTRRSPK"
     gene            complement(2783..3859)
                     /locus_tag="VIH70_14120"
     CDS             complement(2783..3859)
                     /locus_tag="VIH70_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8117544.1"
                     /translation="MDEVARIVLALETPDVAEEVMHFLDRTGRARVVGTAVDATQLAE
                     AVRQLEPDAVVAAPSLVPSRGTLNGSALLAVDTSQSVGALRRAIRGGGTGFYLWPAER
                     EELALAAARVGPKPEETTGERAQALAVYAPRGGAGATFVATHLAAAFARRDRRCILID
                     LDVVFADGSAAIGVPADEELRTVADLVPLGDEVASQHVEELVWRHPAGFSALLAPGDR
                     QRFPRVGAETYGAAISAARRSCDVVVLHVPRALDEVARTGLAVADRVVVVLGLDVLSF
                     RDAKRAIEIAALDGRCSFVVNRASRSEITPNDVDRVFGHPAIAVIPSDRTVGRAQDHG
                     RLVPMRGRIGRALDGLARQLLEAP"
     gene            4051..4407
                     /locus_tag="VIH70_14125"
     CDS             4051..4407
                     /locus_tag="VIH70_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8117545.1"
                     /translation="MAGIEIRRFEQADESRTFGHGSFDVVTIDSVTIGRASYEPGWIW
                     SEHVGKPAGEKLCQVEHVGLVVSGRTGVRMADGTEAELTPGDLFAIGPGHDSWVIGDE
                     PYVSLHLLGASQYARH"
     gene            complement(4404..4937)
                     /locus_tag="VIH70_14130"
     CDS             complement(4404..4937)
                     /locus_tag="VIH70_14130"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8117546.1"
                     /translation="MLNGERVVLRPIQREDLPRLWELVSDFEVAVLGSPGPVVPRSLA
                     QFEAGFDQDLTKDQKDHAYFGIEVDGELIGEGGLHHIDHFSRACELGIGIGREFWGKG
                     FGQDAVRTLVDYAFEHLNMNRVGLYVLAEDPRAVGAYRKAGFVEEGTIRQHAWVRGRY
                     EDELVMSVLREEWTPRT"
     gene            5038..5292
                     /locus_tag="VIH70_14135"
     CDS             5038..5292
                     /locus_tag="VIH70_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117547.1"
                     /translation="MGSIIDLSEWRERRERDPMTRLERAIARLDALLRRSGSGRLAGR
                     VESELLAITGAVSAGLEDAAAERAERLAERLEHPSARRSG"
     gene            5480..5998
                     /locus_tag="VIH70_14140"
     CDS             5480..5998
                     /locus_tag="VIH70_14140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8117548.1"
                     /translation="MNDPDPRTLTRARQGDLGAFEDLVRQYQADVWRFAYHFTRDRSL
                     ADDVTQEAFLRAFRFLRGFRGDSKFTSWLFRIARNCAMDQIKARNVYVERVTPIAGTT
                     TDPQARVELHAALDAVSAEHREPFMLVEVFGLSYQEAADVLGVRLGTIKSRMHRARRA
                     MMDALAVEEEAR"
     gene            5995..7944
                     /locus_tag="VIH70_14145"
     CDS             5995..7944
                     /locus_tag="VIH70_14145"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8117549.1"
                     /translation="MNHEQAERLLSSRIDGERLSTRVSASLEQHLETCAECRAFERDA
                     YRLREAARFEVAPAVPDLVDRVMTAVGDEAERPRSRLRVVRPAGSPRRWILPRLAPVA
                     AALVVGALIGSLVVGGPWQRPETGSALAATEVTEGISAAAVRLDAYQATFAIDERDLS
                     PEVPERHLTMRVSFESPEHFRLDVVDRTVYPMAATPTDLRLVVDGSTWYAAGPAPCPT
                     ATCPQRESVVRNRLPFSTAAPIPTDLVLPISTLADADRLTVLGRGTVLGRPAVRVEVP
                     FERAEPLFPFLAIGGDWRPFFPNDRVRIWLDERNWFPLRWDVYPATGAERDAWALRFG
                     LPDEPSLRPVFSVRALRVSLDSPEAGAFDIPPTNSSQDQGARVVELGDVRRAIGFEPV
                     APSTVGGLDRYRVVLPRDDAGGTLVAYADGLAFLNLGETRSWNGDAPFGPVGVQAEEI
                     SLTGGGVAYYEPASRDHGRRVAIHAAGTDLYLESNLPRSELLAAAAALPVQGLPMPDA
                     WTHRRVGAATAERVSLETARASVPFPIELPTALPAGFGLASVELVDDGFGAGVTLYLR
                     DEDTDAGIGTIRLHLEGASALPPATSAAQSTVEVGHVQGRFTPDRSQLEWIDGGLYRS
                     LDAPGLALEELLAIATSIGGDGTDG"
     gene            7941..9140
                     /locus_tag="VIH70_14150"
     CDS             7941..9140
                     /locus_tag="VIH70_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY8117550.1"
                     /translation="MSRRRAAGVAAGVAMGFALGATLTLAIQGWANLPDPGSVAQPRV
                     PRTEPEPPEAFLVWTSGGMPEGFRAAVRRLDGIQRSVVVASDNTWLDGSWSEQGEVVD
                     DPRRGFAIPLEVAAVEPEEFAPFLPPADRSDALALADGQGILGESSARLRGLGPGAVM
                     RFGDVRIEVAAILPDELVGAHELMVSREVGRSIGVTKDRYALVVPDHAATAPAVERLL
                     DPILPADPPSKVRAPGDTPYFRQGDAVLPPVRLKLLFGEFQARPEPGNPGFLDVDPAW
                     VKQNIDTQHVPLLGTVTCNVSLFPQLRGVVHELIDLGLQDAIHSYSGCYSPRLINRVP
                     SAGLSHHSWGIAADLNVRQGNLFGQIPHQDPRLVDVFERWGFVWGGTFIVPDGMHFEY
                     RREPADA"
     gene            complement(9343..10152)
                     /locus_tag="VIH70_14155"
     CDS             complement(9343..10152)
                     /locus_tag="VIH70_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEY8117551.1"
                     /translation="MEAAFFDLDKTIISRSSSLALSRPMYRAGMVTRGQLVRGAYAQL
                     VYALVGADEKKMDRLKEGMLQLTRGWDRHEVERLVEEVLIEVIDPFVYQEALDLMELH
                     HSEGRDIYIVSSSPEEVVRPLAGHFGVAGVLATRARIEEGRYTGELEFYCYGVAKAEA
                     IRSLAQDRGIDLESSYAYSDSSTDLPMLRTVGHPVAVNPDRDLRKEAETQGWDIRNFR
                     RPVRLRTRIVQTAAHPRTRVAAGLVAASAAAAIVLWLVVRSRLSGRRSTVA"
     gene            10273..10364
                     /locus_tag="VIH70_14160"
     tRNA            10273..10364
                     /locus_tag="VIH70_14160"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10308..10310,aa:Sec,seq:tca)
BASE COUNT         1676 a         3731 c         3635 g         1655 t
ORIGIN      
        1 gtggcgcgct cgcacgtagc gagcacggac gtaccaggac aagagcatcc cggttcacct
       61 cctctcagtc gatcgaacgg acggtggtca gcaagaccgg gaccaccgtc aggaggagga
      121 acgcggggag gacgaggaac atgaaagttg actatctggc accgtcagct ggcaagccgt
      181 gtcgatatcg gttctcgccg gagcgactgc cacggccccc gggcgttgtt ggttgacgtg
      241 ggctggccct gcctggagca tcgccgcacg atgggagacg agcactggta ccggggtgcg
      301 gcatgggaca aggtcgcgcg agccgagttc gaggaacgcc tgtcgcgggc ccggtcacaa
      361 aggccccagt acatgcggat caaggccacc cacctgctcg actccgacga ccccatcgcc
      421 agggccgggg gcgtcgactt gatgcgtcgg ctgatcgatg agtacccgga tgacagactc
      481 tcagtgccgt tcgctcacga gcagctggct atgtacttcg agaagcaagg cctccgtgag
      541 gaggcgaaga acgagtaccg gcaaagcatc gccaaggcac tacccaaccg cagcgggacg
      601 cgttggccag acctctccct cgtcgagtta ctgatcgacg acggtgaggt cgaggaggcg
      661 ttggaacgct gggagatcgc tcggacgatg tcgcgcggtc aggacatctt cccggccgta
      721 gccttcgact tccaccggat cgaggccaag gtctccctca ccatcggcga ccagcaggcg
      781 gcggaagcgg ccgcacagaa ggcattggcc gcggccgccg cgacgcagtc caacgctctc
      841 caccaccgaa acctcggtct cgtggagagc gatgaggcga cgcttgcgtt cttgaggagg
      901 actgcgagaa gggcctgaag gcgagcagac tccggaacac catgattcag tgtctggtac
      961 cgcccacccc agcgagaggt atcgaccaga acttccgcat attcacggga cccggagcga
     1021 aagaggcggc acgatcgcgt ggcagccgag gccgtccgga agtcgcaggg tcgggggatg
     1081 tagggaagat tggtcgactc ggagcccggg cggggcgttc atggtgaatg accgtctcct
     1141 ccccttcgat acaccgccct gcacctctcg ttcggcggac caagctcctg cgggtacatg
     1201 cggaaggggc gaaggaagcc tcacacgcgg gagcgcgtgg acattatcga catctcatcc
     1261 gacatcccga gccaggcgct ccgcacgccg caaccactcc ccaggcagta tcgagtcacg
     1321 atcgccccac cgacccttgt gtccggagac gtgcagcaac tcagcgagct cgcggccctc
     1381 agccagaaac tggcgatccc tgtcgccttc tggaccttgc atcagctttg gcgagagcgc
     1441 ctcaaccatg tcgagcatgc gggccgcggt ccttacccgg cgagcatagc cacgtcgcac
     1501 cgtctttccg cagatcggac atgtcgtcgt catgcggctc ggttcttata tgcgagcggc
     1561 tgctgcgtca atcgctcaaa cgcctcgttg gtctctcccg cttcacgcgc caggcgcgtg
     1621 acaagtcatg acgcctcgta gcgcaccccg cctggatccg cggacaacgc ttctaatccg
     1681 tacgcctcaa aggacagaac gctagtgcac tcagctgagg tcctcctcga gcaacctatg
     1741 ctgtggttat gacatcccga acgatgtcgg ttggaggggc cgtgctgctc cttgctgttg
     1801 caatcggcgt tgctgtggct gtccccgatg ggcctctggc ctgtccagtc gataccgttg
     1861 tgctacgggc ggagggggta atctattgcg agcgcttcgt ccaaccagaa gccgcgccgt
     1921 cgcatgccga gttcgtcgag gcacggcggg actcaaagac cgccgagcgc tggctaatcg
     1981 tcggggtggg tgccctcggt accgccctac tggtcacctt cgggctccgc gcctcgtcgc
     2041 aacgcctctg atcctgcgca gaacttgagt gagaacatgc acttctctgc cacaggaaca
     2101 tagcaggtca gaggcctatt tcgagcctcc ttgggggcaa atctgccccc acaggcacga
     2161 ctggattgtt gaccggtcgt tcagttcggc ttaggtagca ttttcgacga gtccaaccta
     2221 atcctccgct tgactaaaca tggcccagaa gtgttcccac tcctccgggt cgatgtcgtc
     2281 ctggtggtca atcgcaagtt gcgtgaagaa ctcgtacacg tcctcatcgt catagatgcg
     2341 atagatctgg tcattgctga tcgctgcgtt gattaggtca cgcgactgtt ccgccgaaaa
     2401 gtccgggtat gcctccaatt gccggatcgc tgcatgggtc ttcacgaaat tgtagctacc
     2461 gacaagatca gcgattgcga gttccttttc gagttccgag gcgagcttga tatggggata
     2521 gtatttcttg aagagcgaag tgagattctt gtacaggatc actttggact tcttcttgtt
     2581 ggcccattct tgactgagcg cgcctccgcg agcgcgctct gcgccagcat gtggccctgc
     2641 tcccgcagga gcgacagcgc ctcctcccgc acccgcagcc ggcgctcggc gcgcggcatc
     2701 gcgtcgagct gccgggcatc cacccggccg atcagccgct cgtgcaaggt ccgccgaacg
     2761 tccacgagcg catccgcccg attcacggtg cctccagcag ctgtcgggcg agcccgtcga
     2821 gcgctcgtcc gatccgccca cgcatcggca ccagacgacc gtgatcctgg gctcgaccga
     2881 cggtacgatc cgacgggatc acggcgatcg ccggatgccc gaagacgcga tcgacatcgt
     2941 tcggcgtgat ctccgatcgg ctcgccctgt tgaccacgaa cgagcaacgc ccgtccagcg
     3001 ccgcgatctc gatcgctcgt ttggcgtctc ggaacgagag cacatcgagc ccgagcacga
     3061 cgaccactcg atcagcgacc gcgagtcccg tccgagcgac ctcgtcgagc gcacgcggga
     3121 cgtggaggac gacgacgtcg caggacctcc gcgccgcgga gatcgctgcg ccgtaggtct
     3181 cggcgccgac ccgtgggaac cgctgccggt caccgggtgc gaggagcgcc gagaatcccg
     3241 ccgggtgacg ccagacgagc tcctcgacgt gctgcgacgc cacctcgtcg ccgagcggca
     3301 ccaggtcggc gacggtcctg agctcctcgt cggccggaac gccgatcgcg gccgagccgt
     3361 cggcgaagac cacgtcgaga tcgatcagga tgcatcggcg atcccgcctg gcgaacgcgg
     3421 ccgcaaggtg cgtcgcgacg aacgtcgcgc cggcgccgcc acgcggcgcg tagaccgcca
     3481 acgcctgcgc acgctcaccg gtcgtctcct ccggtttggg tccgacgcgt gcggcggcga
     3541 gcgcgagctc ctcgcgctcg gccggccaga ggtagaagcc ggtcccgccg ccacggatgg
     3601 cgcggcgcaa cgcccccacc gattggctcg tatcgacggc gagcagcgcg ctgccgttca
     3661 gcgtgccgcg cgagggaacg agcgagggtg cggcgaccac ggcgtcgggc tcgagctgcc
     3721 gaacggcctc ggcgagctgc gtggcgtcga cggcggtccc gacgacgcgg gcgcggccgg
     3781 tgcgatcgag gaagtgcatg acctcctcgg ccacgtccgg cgtctcgagc gccagcacga
     3841 tccgcgcgac ctcatccatc ctcgctccac gtccaggggg aagcgccgac cgaccgaatg
     3901 aggggaggat cttgcttcgg ggagtcgcgg cgaaccgcgt aggcgaacag tacgcggcgg
     3961 cgcgagttcg agacaatcac caggccgacc gattcgtcgt tcttgtcggg ggatgtgact
     4021 cggcgccggg agcggaatga ggaggagccc atggcgggga tcgagatccg caggttcgag
     4081 caggccgacg agtcacggac gttcggacac ggttcgttcg acgtggtgac catcgacagc
     4141 gtcacgatcg gacgcgcgag ctacgagccc ggatggatct ggtcggagca cgtcggcaag
     4201 ccggccggcg agaagctgtg tcaggtcgag catgtcgggc tcgtggtctc ggggcggacc
     4261 ggcgtcagga tggcggacgg gaccgaagcc gagctcacgc ccggcgacct gttcgcgatc
     4321 ggtccgggtc acgactcatg ggtcatcggc gacgagccgt acgtctcgct ccacttgctg
     4381 ggtgcgagcc agtacgcgag gcactaggtc cgcggcgtcc actcctcgcg caggaccgac
     4441 atcacgagct cgtcctcgta ccggcctcgc acccatgcgt gctgccggat cgtgccctcc
     4501 tccacgaacc cggccttgcg gtacgccccg acggctcgcg ggtcctccgc cagcacgtag
     4561 aggccgaccc ggttcatgtt caggtgctcg aacgcgtagt cgaccagcgt ccggaccgcg
     4621 tcctgcccga accccttgcc ccagaactcg cggccgatcc cgatgccgag ctcgcatgcg
     4681 cggctgaagt ggtcgatatg gtggaggccg ccctccccga tcagctcgcc gtccacctcg
     4741 atcccgaaat aggcatgatc cttctgatcc ttcgtcaggt cctgatcgaa gccggcctcg
     4801 aactgcgcga gcgagcgggg cacgaccggc cccggactcc cgaggaccgc gacctcgaag
     4861 tccgagacga gctcccacag tcgagggaga tcctcccgct ggatcgggcg gagcacgacc
     4921 cgttcaccgt tcagcatcgc gcatcacggc gtcgatccag cccacgcagg gagcctagcc
     4981 catcgtttcc gggcacaggt ccgaggaagt gtcggaccgg gggtctacga tcgcgacatg
     5041 ggttcgatca tcgacctctc cgagtggcgc gagcgccgcg aacgcgaccc catgacccgc
     5101 ctggagcggg ccatcgcccg attggacgcc ctgctgcgcc gcagcggctc cggccgcctt
     5161 gcgggcaggg tcgaatccga gctgctggcc atcaccggcg ccgtctcggc cgggctcgaa
     5221 gacgccgcgg ccgagcgcgc cgaacgcctc gccgagcgcc tggagcaccc ctccgcccgc
     5281 cgctccggct aggcacgaag ccggccgcct tctcgcacgc cgggaaccca tccggccctt
     5341 ggccggtcag caagggcgac cggaagcttc cgggagggag gcgccccggc gcacgctcgc
     5401 gcccgaaccc tgcctgcgtg ccggtgcggg gctacgctga gtggccggcc acggggacag
     5461 gcgaacggga agggaaaggg tgaacgatcc ggacccacgc acgctcacgc gggctcgcca
     5521 gggcgacctc ggcgcgttcg aggacctcgt gcgccagtac caggcggacg tctggcgctt
     5581 cgcgtaccac ttcacgcgcg accggtcgct ggccgacgac gtcacacagg aggcgttcct
     5641 gcgcgcattt cggttcctcc gcgggttccg tggcgactcc aagttcacga gctggctctt
     5701 caggatcgcg cgcaactgcg cgatggatca gatcaaggcg cgcaacgtct acgtcgaacg
     5761 cgtcaccccg atcgccggga cgaccaccga cccgcaggcg cgcgtcgagc tgcacgccgc
     5821 gctcgacgcg gtctcggccg agcatcgcga gccgttcatg ctcgtcgagg tgttcggcct
     5881 gtcgtaccag gaggcggccg acgtactcgg cgtccgcctc gggacgatca agagccggat
     5941 gcaccgggcg cgtcgagcga tgatggacgc gctcgcggtc gaagaggagg cacggtgaac
     6001 cacgagcagg ccgagcgtct cctgtcctcg cgcatcgatg gcgagcggct gtcgacgcgg
     6061 gtgagcgcgt cgctcgagca gcacctcgag acgtgcgcag agtgccgggc gttcgaacgc
     6121 gacgcgtatc ggttgcgcga ggccgctcgg ttcgaggtcg cccccgccgt ccccgacctg
     6181 gtcgaccggg tcatgaccgc cgtcggcgac gaggccgagc gccctcgctc gcggttgcgg
     6241 gtggtccggc cggccggatc cccccgccgt tggatcctcc cgcggctcgc tcccgtggcc
     6301 gcggcgctgg tcgtgggggc gctgatcggc agcctggtcg tcggcggacc ctggcaacgg
     6361 cccgagaccg ggtcggcgct cgccgccacc gaggtcaccg agggcatctc ggccgccgcg
     6421 gtgcggctcg acgcgtatca ggcgacgttc gcgatcgacg agcgggacct gtcccccgag
     6481 gtgcccgaac gccacctgac catgcgcgtt tcgttcgaat cgcccgaaca cttccgcctg
     6541 gacgtcgtcg accgcacggt gtacccgatg gcggccacgc cgaccgacct gaggctcgtg
     6601 gtggacggct cgacgtggta cgcggccggg ccggcgccct gccccacggc gacctgcccg
     6661 caacgcgagt ccgtcgtgcg caaccgcctg ccgttctcga cggccgcacc gatccccacc
     6721 gacctggtgc tcccgatctc gaccctggcc gacgccgacc gcctgacggt gctcggtcga
     6781 gggacggtgc tcgggcggcc ggccgtacgg gtcgaggtcc cgttcgaacg cgccgagccg
     6841 ctgttcccgt tcctggcgat cggcggcgat tggcggccgt tcttcccgaa cgatcgcgtc
     6901 cggatctggc tcgacgagcg caactggttc ccgttgcgtt gggatgtcta cccggcgacc
     6961 ggcgccgagc gcgacgcctg ggcgctccga ttcggcctgc ccgacgagcc ctcgctgcgc
     7021 ccggtcttct cggtgcgggc gctccgcgtg agtctcgact cgccggaggc cggcgcgttc
     7081 gacatccccc cgacgaactc ctcgcaggat cagggcgctc gagtcgtgga gctcggcgac
     7141 gtgcgacgcg cgatcgggtt cgagcccgtg gcgccgagca cggtcggcgg gctcgatcgc
     7201 taccgggtgg tgctgccacg ggacgacgcc ggcgggacgc tcgtcgccta cgcggacggc
     7261 ctcgcgttcc tcaacctcgg cgagacacgc tcgtggaacg gcgacgcgcc gttcggaccc
     7321 gtcggcgtgc aggcggagga gatctcgctc accggcggcg gtgtcgcgta ctacgagccg
     7381 gcgtcgcggg accacggccg ccgcgtagcg atccacgcgg ccgggaccga tctgtatctc
     7441 gagtccaacc tgccgcgttc ggagctgctg gcggccgccg ccgcgctgcc ggtccagggc
     7501 ctcccgatgc ccgacgcgtg gacccatcgc cgggtgggcg ctgcgaccgc cgaacgcgtc
     7561 tcgctcgaga ccgcccgggc gtccgtcccg ttcccgatcg agctcccgac cgcgctcccc
     7621 gccgggttcg ggctcgcgag cgtcgagctc gtcgacgacg ggttcggcgc cggtgtgacc
     7681 ctgtacctgc gcgacgagga cacggacgcc ggcatcggca cgatccgcct gcacctcgag
     7741 ggcgcgagcg cgctgccgcc ggcgacatcg gcggcgcagt cgacggtgga ggtcggccat
     7801 gtgcagggcc gattcacccc cgaccggtcg cagctggaat ggatcgacgg cggcctgtac
     7861 cgctcgctcg acgctcccgg gctcgcgctc gaggagctcc tggcgatcgc gacctcgatc
     7921 ggcggggacg ggacggacgg atgagccgcc gccgggccgc gggggtcgcg gcgggtgtcg
     7981 cgatgggctt cgccctgggc gccacgctca cgctcgcgat ccaggggtgg gcgaacctgc
     8041 ccgacccggg gtcggtggcc caaccgaggg tcccgcggac cgaacccgag ccacccgagg
     8101 ccttcctggt ctggacgtcc ggtggcatgc ccgaagggtt ccgagcggcc gtccgtcgcc
     8161 tcgacgggat ccagcgctcg gtggtggtcg cgagcgacaa cacgtggctg gacgggtcgt
     8221 ggtcggagca gggcgaggta gtggacgacc cgcgtcgggg cttcgcgatc ccactcgagg
     8281 tcgcggcggt cgagcccgag gagttcgcgc cgttcctgcc gcccgccgac cggtccgatg
     8341 cgctggcgct cgccgacggc cagggcatcc tgggcgagag cagcgcccgg ctccggggtc
     8401 tcgggccggg ggccgtgatg aggttcggcg acgtccggat cgaggtggcg gcgatcctgc
     8461 ccgacgagct ggtcggcgcg cacgagctga tggtctcccg cgaggtgggc aggtccatcg
     8521 gcgtcacgaa ggaccgctac gcgctggtcg tgcccgacca cgcagcgacc gccccggcgg
     8581 tcgaacggct cctcgacccg atcctgccgg ccgaccctcc ctcgaaggtc cgcgcccccg
     8641 gcgacacgcc gtatttccgg cagggcgacg cggtgctccc gccggtccgg ctcaagctgc
     8701 tgttcggcga gttccaggcg cgacccgaac ccggcaaccc cggcttcctc gacgtcgacc
     8761 cggcgtgggt gaagcagaac atcgacaccc agcacgtccc gctgctcggg acggtcacct
     8821 gcaacgtgtc cctgttcccc cagctgcgcg gcgtcgtcca cgagctgatc gacctggggc
     8881 tccaggacgc gatccactcg tactccggct gctactcgcc tcgactcatc aaccgggtcc
     8941 cgagcgccgg actgtcgcac cactcctggg gcatcgcggc cgatctgaac gtgcggcagg
     9001 ggaacctgtt cgggcagatc ccgcaccagg accctcggct cgtggacgtg ttcgagcggt
     9061 gggggttcgt ctggggaggg acgttcatcg tcccggacgg gatgcacttc gagtaccggc
     9121 gcgaacccgc ggacgcctag cttcgcaggg gccgcacatg cgcgaaggga gcagcgagca
     9181 tgcgctgtct gcactttgcc cggtgtccgg cggccggaac cacagggccc gagcatgcct
     9241 cccgcggcaa acgcgcgtgg gtttctgctc ccttcgcggt cgcccgaagg cgacgcggcc
     9301 atcgtacccc tggcctcctc ggcgcgcgaa cgcgatcggg cactacgcca cggtggagcg
     9361 acgaccggac agccgcgaac gaacgacgag ccacaggacg atagcggcgg cggcgctcgc
     9421 ggcgaccaaa ccggcggcga cgcgggtcct ggggtgcgcg gccgtctgga cgatccgggt
     9481 ccggagccgg accgggcgcc ggaagttccg gatgtcccag ccctgagtct cggcctcctt
     9541 ccgaagatcc ctgtccgggt tcaccgcgac ggggtgaccg acggtccgca acatcgggag
     9601 gtcggtcgag gagtcgctgt acgcgtacga ggactcgaga tcgatcccgc gatcctgggc
     9661 gagcgatcgg atcgcctccg ccttcgcgac gccgtagcag tagaactcga gctcgcccgt
     9721 gtagcgcccc tcctcgatcc gcgcgcgcgt cgcgaggacc ccagcgacgc cgaaatgccc
     9781 tgcgagcggg cggacgacct cctccggcga cgagctcacg atgtagatgt cacgtccctc
     9841 ggagtggtgc agctccatca ggtcgagggc ctcctggtat acgaacgggt cgatcacttc
     9901 gatcaggacc tcctccacga ggcgctcgac ctcgtgccgg tcccagcccc gcgtgagctg
     9961 gagcatccct tccttcagcc ggtccatctt cttctcgtcc gcgccgacca gggcgtagac
    10021 gagctgcgcg tacgcgccgc gcacgagctg tccgcgcgtg accatcccag cgcggtacat
    10081 cggccgggac aaagcgagcg aggacgagcg cgagatgatc gtcttgtcga ggtcgaagaa
    10141 cgccgcttcc ataccctcat ggtatcggtg gccggtgccc cgcccaggag cccgtgcgac
    10201 ggcggatccc acgcgcgacg aatggtggcc gagcaaggtt cccttcggag cctcaccgct
    10261 atcctcgctc gcggaggcgt cagggcacct ggtgggcccc ccggtcttca aaaccggtgt
    10321 gggggcgaga gctctcaggc gggttcgatt cccgtccgcc tccgctattg aagcgtcctc
    10381 tgaccagtcg cttcgggaag aggcgggcgt aggagggcgc gggaaggggc tcgatcggag
    10441 ctccggagac cgcgtctacc acacaagtct ttcggtgttc gcttcgcgcg acaccggacg
    10501 agtcttccga ccgccctccg gaagaccgga caatcttcag ccgccgtcct ctcgaagaga
    10561 ggagtgagag cgtcgacgac ctcgtccagc gtgaacccgg cccttcagct ctcttgcgaa
    10621 tcggacccgc tcaaccgcgg ccgtctcgta gacgcggtag cccgagggca gcctgtctgg
    10681 tggcggaaga actccgc
//
LOCUS       DATBRK010000487         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1876568, whole genome shotgun sequence.
ACCESSION   DATBRK010000487 DATBRK010000000
VERSION     DATBRK010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876568"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VIH70_14165"
     CDS             complement(<1..296)
                     /locus_tag="VIH70_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8117552.1"
                     /translation="MLLKDKVAVVYGAGGAIGGAVARAFAQEGARLFLNGRLRAPVEA
                     VANDVVAAGGSAEAAECDALDERAVDEHLRSVIDGAGRVDISFNAVGIPDTNIVG"
     gene            445..1491
                     /locus_tag="VIH70_14170"
     CDS             445..1491
                     /locus_tag="VIH70_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8117553.1"
                     /translation="MRLDEAVTTDLISRARAGDPEAFRKLTEPYRRELQVHCYRMLGS
                     FQDAEDAVQDTMLTAWRGLGGFEGRASIRTWLYRIATNRCLDALRSASRRPAKEWDIA
                     EYEPPEPTGLGDVVWLQPFPDALLEGAIDGPLAPEARYEQTEAISLAFVTALQVLPPR
                     QVAVLILRDVLGFHADEVADMLDSTVESVTSALKRARANLHRRLPPAAERGPAPDSPS
                     EQAIVARFVGAWESADVDALVALLTDDVFVSMPPVPFEYEGRDAVERFCASVFGSGRR
                     FDLVPTRANGQPAFGAYLRAPTGIRHGTGLYVLTLAGDRICAMTRFDNSVLPWFGLPR
                     SLPSRSPGGSAISR"
     gene            complement(1481..>1848)
                     /locus_tag="VIH70_14175"
     CDS             complement(1481..>1848)
                     /locus_tag="VIH70_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEY8117554.1"
                     /translation="DVDERMQIDGFTFGSIRIDGVDYEHDVVIARGRVRRRKKKASKP
                     FRDAFGHTPLSVEEDIPWDCRRLVVGTGASGALPVMDEVAAEAARREVTLVTVPTREA
                     IRVLDAEPADTNAILHVTC"
BASE COUNT          263 a          697 c          603 g          285 t
ORIGIN      
        1 cccacgatgt tcgtgtcggg gatcccgacc gcgttgaacg agatgtcgac gcggcccgcc
       61 ccatcgatca cggaccgcag atgctcgtcc acggcccgct cgtccagtgc atcgcactcc
      121 gccgcctcgg cggatccgcc ggcggcaacg acgtcgtttg cgaccgcctc gacaggtgcc
      181 cgaaggcgcc cgttgaggaa gagcctggcg ccctcctgcg cgaaggcgcg tgcgaccgcg
      241 ccgccgatcg cccctccggc cccgtagacc accgcgacct tgtccttcag cagcatcgcc
      301 ggctcctttc tgtgccctgg tctgtgccct gggtccaccc ggtgatgcag acgccgcgac
      361 ggtcgcaaag cgggcgcccg ccgaccgccc agtttcgccc ctcggccggc gtccatacag
      421 gagtggacaa cgagagcgag agacgtgagg ctggacgaag cggtgacgac ggacctgatc
      481 tcccgggcgc gggccgggga ccccgaggcg ttccgaaagc tgaccgagcc ctaccggcga
      541 gagcttcagg tgcactgcta tcggatgctc ggatccttcc aggacgccga ggacgccgtt
      601 caggacacga tgctgacggc ctggcgaggg ctcggagggt tcgaggggcg agcctcgatc
      661 cgaacctggc tctaccggat cgccaccaac cggtgcctcg acgcgctgcg ctcggccagc
      721 cgacgcccgg ccaaggagtg ggacatcgcc gagtatgaac ccccggagcc gaccggactc
      781 ggcgacgtcg tttggctcca gccgttcccc gacgccctgc tggagggtgc gatcgacggg
      841 ccgctcgccc ccgaggcccg ctacgagcag accgaggcga tctccctcgc cttcgtgacg
      901 gcgctccagg tcctgccgcc tcgccaggtc gccgtgctca tcctgcgcga cgtcctcggc
      961 ttccacgcgg acgaggtggc cgacatgctc gactcgaccg tcgagtcggt gaccagcgct
     1021 ctcaagcgag cccgcgccaa cctgcatcgc cgcctgccgc cggccgccga acgcggaccg
     1081 gctcccgact caccctccga gcaggcgatc gtggcgaggt tcgtcggcgc gtgggagtcc
     1141 gccgatgtcg acgcactggt ggcgcttctg acggacgacg tcttcgtctc gatgccgccg
     1201 gtgcccttcg aatacgaggg ccgagacgcc gtggagcgct tctgcgccag cgtcttcggc
     1261 tcgggccgca ggttcgacct cgtgccgacg cgcgccaacg gtcagccggc gttcggggcc
     1321 tacctgcgcg ctccgaccgg catccgccac ggcaccggcc tctacgtcct caccctcgcc
     1381 ggcgaccgga tctgcgccat gacccgcttc gacaacagcg tgctcccatg gttcgggctc
     1441 ccgcgatcgc tcccgagtcg atcaccggga ggttccgcga tcagcaggtg acgtgcagga
     1501 tcgcgttcgt gtccgcgggc tcggcatcca gcactcggat ggcctcgcgg gtcgggacgg
     1561 tcaccagcgt cacctcccgg cgggcggcct cggccgcgac ctcgtccatc acgggcaggg
     1621 ctccgcttgc tccggtgccc acgaccaagc gcctgcagtc ccaggggatg tcctcctcga
     1681 cggacagcgg cgtgtgtccg aacgcatcgc ggaacggctt cgacgccttc ttcttccgcc
     1741 tacggacccg cccgcgagcg atcacgacgt cgtgctcgta gtccactccg tcgatccgga
     1801 tcgagccgaa cgtgaacccg tcgatttgca tgcgctcgtc gacgtcag
//
LOCUS       DATBRK010000488        13736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1877503, whole genome shotgun sequence.
ACCESSION   DATBRK010000488 DATBRK010000000
VERSION     DATBRK010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13736
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877503"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VIH70_14180"
     CDS             complement(<1..500)
                     /locus_tag="VIH70_14180"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY8117555.1"
                     /translation="MNPEFLSRLQFALTASFHFIFPPISMGLGILLVWMAWKHVRTGD
                     PVWRQSSVFWVKVYGLVFALGIATGLVQEFAFGMNWSQYSRFVGNVFGSLLAAEGVFA
                     FALEGGLLGLMLFGGDRLGPRMWLLATFLVVFGAHFSALWILMANSWMQTPQGFTTHM
                     TEWGNQA"
     gene            complement(615..2147)
                     /locus_tag="VIH70_14185"
     CDS             complement(615..2147)
                     /locus_tag="VIH70_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005515715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8117556.1"
                     /translation="MILVAAVANLNLAVANVALPSIGAAFDSSQTTLNLIAVGYSLGL
                     AASVLWLGALGDRYGRKLMLILGTGLAIPFALLAAYAPSDSVLFVARLGGGLAAGMAF
                     PTTLALITALWSGPGRTKSIALWSGIGGAVAALGPMFSGLLLEHFWWGSVFLITLPLA
                     VVALVLAVRLVPAHVNEGIDPVDNLGGILSALLVGALILGINFAAVPNEGALVAGLAI
                     IALASGIAFLIRQRRAANPLYDLHVAGRRVFWVAACAGIIVFGSLMGAMFVGQQFLQD
                     VLAYSTFDAGVAILPAAFLMVIVAPRSAKVVEARGARFTLLTGYAFCLLGFLTMLLLW
                     GEGSPYWEVGLGYALIGVGVGFAGTPASHSLTGSVPVERAGMASGTADLQRDLGGAIM
                     QSIFGTLLAAGYAASFTSAIAASPNAQKVSDSVEAQLTKSFAGAEAIAQQYSKYADQI
                     TSAAKASFLSGDQWAYIAGIVAVGLGAALVFFAFPRKEDERRLLAEYQREDSSSASRV
                     AS"
     gene            2544..2972
                     /locus_tag="VIH70_14190"
     CDS             2544..2972
                     /locus_tag="VIH70_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEY8117557.1"
                     /translation="MSIGPVEYMIVAFPGNQFKGEIVPALKELVDAGTIRIIDLAFVM
                     KDADGAVGTAELSDLDSEVYKAFDALSPETMGLLNQDDLAAAGEELDPNSSAALLVWE
                     DIWATKLRDAIVNAKGELLDLERVPYEVVQAAVDFAEANR"
     gene            2996..3289
                     /locus_tag="VIH70_14195"
     CDS             2996..3289
                     /locus_tag="VIH70_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY8117558.1"
                     /translation="MRRGPGLVGMAARTAVVAGTATAVSGRVARRQANRYADQDAQYV
                     QQQTAAATQGAYSAPAPPEEDATTQIQQLAQLHASGALTDEEFAAAKAKILGI"
     gene            complement(3372..3770)
                     /locus_tag="VIH70_14200"
     CDS             complement(3372..3770)
                     /locus_tag="VIH70_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117559.1"
                     /translation="MQSERQPSYWAAGWATFAGLMLILLGLFHAIAGLSEVVDPDAYA
                     VTENYVFKFSSSAWGWIHLIGGIIVFFAGFAIFRGAVWGRTVGVIIAIVSAAAAFAWL
                     PYAPVSAIAIIAIDIAVIWALTAHGRDVTA"
     gene            complement(3800..3973)
                     /locus_tag="VIH70_14205"
     CDS             complement(3800..3973)
                     /locus_tag="VIH70_14205"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY8117560.1"
                     /translation="MAASQQSQPEPQAAPPPAAAPAPQDDMAQIQQLAAMKDQGLLTQ
                     EEFEAKKKQILGI"
     gene            complement(4054..4587)
                     /locus_tag="VIH70_14210"
     CDS             complement(4054..4587)
                     /locus_tag="VIH70_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117561.1"
                     /translation="MTIGPVQLLVLGFDKPDFRGEIIDEFERLRENDTVRVIDALVVF
                     KDAEGDVAALKGSQLSKDEAEEFGAVVGGLLGLGAGGEEGMEAGAIAGAAAASDGVDV
                     FDEEEAWDVLEEIPNDAAAALILLEHRWAIGLRDAIVRAGGFPIGGHFISPLDLIEVG
                     LMSAEEAEALAASGQGT"
     gene            complement(4607..5374)
                     /locus_tag="VIH70_14215"
     CDS             complement(4607..5374)
                     /locus_tag="VIH70_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HEY8117562.1"
                     /translation="MVAETTALSDRDLLQMMDLVRGADSVELKLTIHESARAEAARAL
                     GVDPLDSQMRQVFFFDTPGLSLNAAGVVVRARRRQNEQGDTVVKLRPVIPGDLPEDLR
                     AMETFNVEVDAMPGGFVCSASFKGAADNTAIRRVASGVLPIRKLFSKQQRAFYEAHAP
                     DGIELDSLTTLGPIPIIKVKITPEGFGRKLVGELWMYPDGSQIVELSTKARPDEAFQV
                     AAEARALLLSKGVDLSSEQQTKTKTALEFFSGAAQTA"
     gene            5585..7876
                     /locus_tag="VIH70_14220"
     CDS             5585..7876
                     /locus_tag="VIH70_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8117563.1"
                     /translation="MGGTSVRVRQATRGRGPEPAAARRPAFDVTEAKLFRPPVRPGIV
                     RRNALVDRLRSAEAASVVAITAPAGYGKTTLLAEWAERDRRPFAWVSVDEADSDPVVF
                     LTHVAVALHRIEPIEPHVLESIASPTGSNSARVLSRLSSALARRTRPFVLVLDDLDRL
                     AEPLITDAVSRIAGSIPEGSALALAARALPDVGLPRLRADGRLEEVGVDDLALGPPQA
                     YRLLRAAGVRATRIEAAELTDATEGWPVGLYLAALSLQEQRRSTMPPIRFTGDDRFVV
                     DYVRSEVLGRLSRERQRFLTRTSILDRLNGPLCDAVMERSGSARTLESIERQNLLLVP
                     LDRQRAWYRYHNLFRDVLRAELARREPEAIPELHRRAADRFEEAGMLEEAFVHAHSSG
                     DEERAAGLLQRVGLAAYRAGRIATLRRWLDLLGDDAISRNPGLALMASLIAALGGDPV
                     AAERWSKATEGVRLTAPSLHGTASLRSSEAIVHATMSRGGTDAMLRAAEVAVREETGT
                     SPYRAAALGLLGLATMIRGDEEVADTLFADASEVGLRIGGMPIASLALAERSLIATSR
                     GDGTAADDLAERARIVVRDAHLDEHITTGPVHAASARAALRAGDHEAARAELAAAHRL
                     RPILTWAFPTIAVQTRLELARVHVGLSDVPGARTILAEVDEILAHRPDLGTLTTQAVE
                     LRAHIGSVRSGRSPGPTTLTAAELRLLNFLPTHLSFREIGARLFVSPNTVKTQAISIY
                     RKLGVSTRSDAVQAARDLRLLEP"
     gene            7974..8351
                     /locus_tag="VIH70_14225"
     CDS             7974..8351
                     /locus_tag="VIH70_14225"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8117564.1"
                     /translation="MAKHEDALRCMALGDERYVRDVLAMQLTNVDASGLDPKTHALVR
                     LGALVALDASQSSFQWNAATALASGATVDEIVGVLIAVAQTVGLARVASAAPAVALAL
                     GYDIDAALEAHDEGGRTLDINSR"
     gene            8386..8676
                     /locus_tag="VIH70_14230"
     CDS             8386..8676
                     /locus_tag="VIH70_14230"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEY8117565.1"
                     /translation="MLADYDFGDFLWTMFAFFFWFMLIWMFIMVFADIFRRKDLSGGA
                     KAGWVLLIFILPLIGILIYLIARPSIVDAREMSSSAGQMQGSAGYASPDHFA"
     gene            complement(8698..9888)
                     /gene="rocD"
                     /locus_tag="VIH70_14235"
     CDS             complement(8698..9888)
                     /gene="rocD"
                     /locus_tag="VIH70_14235"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEY8117566.1"
                     /translation="MELVERTTAHNYHPLPVVVAEAEGCWVTDVEGKRYLDMLAAYSA
                     LNFGHRHPELIAAAKEQLDRVTLTSRAFHNDQMGPFLRDLTDLCGMDLALLMNSGAEA
                     VETAIKTARKWGYEVKGVAPDRAKVIVAGGNFHGRTITIISFSTDEGSRRNFGPLTPG
                     FEVVPYGDAAALEAAIDDDTVAFLVEPIQGEAGVVVPPEGYLRRARELCTGRNVLLVA
                     DEIQTGLGRTGTTFACEHDGVRPDVFILGKALAGGIVPLSAVVADEAVLGVFRPGEHG
                     STFGGNPVACAIGRRVVAMLRTGEFQEASTRLGAYLHSALRAADLLTVEEVRGRGLWA
                     GIQLVDSALPARCSAERLLEAGVLAKDTQESTLRLAPPLVISQHELEWGLERILPVLE
                     RAAN"
     gene            complement(9972..10652)
                     /locus_tag="VIH70_14240"
     CDS             complement(9972..10652)
                     /locus_tag="VIH70_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8117567.1"
                     /translation="MASAAADAAALADHLAAERFLTMGWSGGGPHALACAALLPDRVR
                     AAATIAGVAPYDAQGLDWTAGMGESNQQEYPLAARDPDELLRWMRPHAEALGAVEPNE
                     IVSELRSVISEVDEAEVTGALGELMATSFHRAFRAGPWGWFDDDLAFVRPWGFELSSI
                     GVPVSVWQGRQDLMVPFAHGEWLVEHIPTARPRLRPEHGHLSLAVGSMGEILDDLLRG
                     RGVDGSVD"
     gene            complement(11023..12399)
                     /locus_tag="VIH70_14245"
     CDS             complement(11023..12399)
                     /locus_tag="VIH70_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8117568.1"
                     /translation="MAVRESVEIDLERVDRLIQEQEAELEPKHRASIAYREIAELHVA
                     GGVASSWQASPPHAIFIERGDGSRIWDIDGNEYVDYHLGYGAMVVGHAHPAIVDAIER
                     QARNGTHFAQPTKHLDVIGENLAERFSLPLWRFCNSGTEATLEAARLMRAKTGRDLLI
                     KIEGTYHGHHDSLMFSVIPDPELMGPRDHPTTVPQAPGIPQAFADLVVVVPFNDLAAA
                     RQGFEEHPGQVAGMIVEPAMMNCGVILPEPGYLQGLADLCHEFGAYLAFDEVKTGVAL
                     AAGGAVEAFGVVPDIVCLAKAIGGGTPCGAIGATEELFRLVVDGEYDMAGTFNGNPLT
                     MATMRATLTEVLTRDAYGTLNHVDGYLKERLSAVIERYRLPAYVTGIGAKGSVIYSTT
                     PVREYRDSLAIDDRITYLAWLFQQNGGVFKSPWSKQETWTLSVRHTDEDAQRYVDNFE
                     AFARALTS"
     gene            12512..12585
                     /locus_tag="VIH70_14250"
     tRNA            12512..12585
                     /locus_tag="VIH70_14250"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12546..12548,aa:His,seq:gtg)
     gene            12619..12692
                     /locus_tag="VIH70_14255"
     tRNA            12619..12692
                     /locus_tag="VIH70_14255"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12653..12655,aa:Lys,seq:ctt)
     gene            complement(12836..13540)
                     /locus_tag="VIH70_14260"
     CDS             complement(12836..13540)
                     /locus_tag="VIH70_14260"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEY8117569.1"
                     /translation="MGETRAEIAPPALTALVFLVVAAVAWWLTIEQSRSMSGMTMDPG
                     TPGAFAVSWVVMMAAMMLPTALPLVFEFARSAEGRRGWQGATGLLGLTYLSVWLAFGL
                     ICYVVRSALPVSLPNQGLVGLVALVLAGLYALTPIKRASEARCRELCALHGIPFNLMR
                     SALVVGVKYGLSCVGCSAALMVAIMIIGMSNLTWILILSAVVLVYKLAPAASMRRTLL
                     LSGALGALGVVYALMT"
BASE COUNT         2184 a         4873 c         4635 g         2044 t
ORIGIN      
        1 gcctggttgc cccactccgt catgtgggtc gtgaacccct gcggggtctg catccacgag
       61 ttcgccatca ggatccacag ggcgctgaag tgcgcgccga agacgacgag gaaggtggcc
      121 aggagccaca tcctggggcc gagtcggtcg ccgccgaaca gcatgaggcc gagtaacccg
      181 ccctccagcg cgaacgcgaa cacgccctcg gcggcgagca ggctcccgaa cacgttgccg
      241 acgaaccgcg agtactgcga ccagttcatc ccgaacgcga actcctggac caggccggtc
      301 gcgatgccca gcgcgaacac cagcccgtag accttcaccc agaagaccga ggactgtcgc
      361 cagacggggt cccctgtgcg gacgtgcttc caggccatcc agaccaggag gatcccgagc
      421 cccatcgaga tcggcgggaa gatgaagtgg aagctcgcgg tcagcgcgaa ctggagccgt
      481 gaaaggaact ccgggttcac gtggctgcct ctccccctcg tctggcgacc gcgcgtgcct
      541 ggggcatctc accgcccgat gcgctgccga gcatcacgcc gatgggatga ttcccggcat
      601 cccgcaacgc agcatcacga cgcgacgcgt gatgctgacg acgagtcctc gcgttggtac
      661 tccgcgagga gccgccgctc gtcctccttc ctcgggaacg cgaagaagac cagcgccgcg
      721 ccgagcccga cggccacgat gcccgcgatg tatgcccact gatcgcccga caggaacgag
      781 gccttcgcgg ccgaggtgat ctggtccgcg tacttcgagt attgctgggc gatggcctcg
      841 gcacccgcga acgacttggt gagctgcgcc tcgacgctgt cggacacctt ctgggcgttc
      901 ggcgaggcgg cgatcgccga cgtgaaggag gccgcgtagc ctgccgccag cagggttccg
      961 aagatcgact gcatgatggc gccgccgagg tcgcgctgga gatcggccgt gcccgacgcc
     1021 atgccagcgc gctcgaccgg gaccgagccg gtgagcgagt gcgaggccgg tgtccccgcg
     1081 aagccgacgc ccacgccgat gagcgcgtag ccgagcccca cctcccaata cgggctcccc
     1141 tcgccccaca acaggagcat cgtgaggaac ccgagcaggc agaacgcgta ccccgtgagg
     1201 agggtgaagc gcgcacccct cgcctcgacg accttggccg agcgcggagc gacgatcacc
     1261 atcaggaagg cagccggcag gatggcgacg cccgcgtcga atgtcgagta ggcgagcacg
     1321 tcctgcagga actgctgacc gacgaacatc gcccccatca gggagccgaa gacgatgatc
     1381 ccggcgcatg cggcgaccca gaacacgcgc ctgccggcga cgtgcaggtc gtacaggggg
     1441 ttcgcggctc gccgctggcg gatcaggaac gcgatcccgg acgcgagggc gatgatcgcg
     1501 aggccggcga cgagtgctcc ctcgttcggc acggccgcga agttgatccc gaggatcaac
     1561 gcacccacga gcagcgccga caggatgccg cccaggttgt cgacggggtc gatcccctcg
     1621 ttcacgtgag ccggcacgag acgcacggcc aggaccagcg ccacgaccgc gagcggcaac
     1681 gtgatcagga agaccgatcc ccaccagaag tgctcgagca gcaggcccga gaacatcggc
     1741 ccgagcgccg cgacggcgcc cccgatcccc gaccacaggg cgatcgattt cgtccgcccg
     1801 gggccggacc agagcgcggt gatcagcgcc agcgtcgtcg ggaacgccat ccccgcggcc
     1861 aacccgccgc cgaggcgggc gacgaacaac accgagtcgc tcggggcgta cgcggcgagc
     1921 agggcgaacg ggatcgccaa gcccgtgccg aggatcagca tgagcttgcg cccgtaccgg
     1981 tcgccgagcg cgccgagcca cagcaccgag gccgcgagcc cgagcgagta gcccacggcg
     2041 atcaggttca gggtggtctg ggacgagtcg aacgccgccc cgatcgacgg gagtgcaacg
     2101 ttcgccacgg cgaggttgag gttggcgacc gccgcgacca ggatcagggt ggtgagaacg
     2161 gtcccctgac gctcgggcgc ggacccggcg gcttgcgtcg ttcccgatcc ccggtcgctc
     2221 acgtcgcgga ccactcctct gccgacgaaa tgcccccgcc tccgccgatc ccggctcgcg
     2281 ggatcgcgtc gaccgcagca tcggacaagg gacctcgctc gacgcgaacc gccctcgatc
     2341 accacgttcg agcgctggct cctgccgaac atcacccatc tgggatgaaa cgggtcaccg
     2401 tcgcgtgatc tacgtttcgg gcggcggtat cgacctgcgg aacggaggtg cccggcgctc
     2461 gccgtcggac ggttcgtgag atagttcgag acccgtggcc gtggcctggc gcggggagag
     2521 cggagcatga aggagggaga cggatgagca tcggtccggt cgagtacatg atcgtcgcgt
     2581 tcccgggcaa ccagttcaag ggcgagatcg tcccggccct gaaggagctg gtcgacgccg
     2641 gtacgatccg gattatcgat ctcgcgttcg tcatgaagga cgccgacggc gccgtgggaa
     2701 ccgccgagct ctccgacctg gactcggagg tctacaaggc gttcgacgcg ctgtcgcccg
     2761 agacgatggg cctgctcaac caggacgacc tggcggccgc gggcgaggaa ctcgacccga
     2821 actcgtcggc ggcgctcctc gtgtgggaag acatctgggc gacgaagttg cgtgacgcga
     2881 tcgtgaacgc gaagggcgag ctcctcgatc tcgagcgggt accgtacgag gtcgttcagg
     2941 cggcggtcga cttcgccgag gccaaccggt agtcgaagaa aggggaacca tcatgatgcg
     3001 cagagggcca ggtctggtcg ggatggcggc acggacggcg gtggtcgccg gcacggcgac
     3061 tgcggtgtcg ggacgggtcg ctcgccgcca ggcgaacagg tacgcggatc aggacgccca
     3121 atacgtgcag cagcagacgg ccgccgcgac ccagggggcc tacagcgccc ccgcgccgcc
     3181 ggaggaggac gcgaccacgc agatccagca gctggcacag ctgcacgcgt cgggggcgct
     3241 cacggacgag gagttcgcgg ccgcgaaggc gaagatcctg gggatctgat caccctcacg
     3301 ccaggcaccg gggactcagg aagcccgccg gagccggctt cctgcctcgg tccggatccg
     3361 aagcgattgg atcaggcggt cacgtcgcga ccgtgcgccg tcagcgccca gatcacggcg
     3421 atgtcgatcg cgatgatcgc gatcgccgag accggagcgt agggaagcca ggcgaacgcc
     3481 gccgccgcgc tgacgatcgc gatgatcacg ccgaccgtcc gaccccatac agccccacgg
     3541 aagatcgcga acccggcgaa gaagacgatg atcccaccga tcaggtggat ccatccccac
     3601 gcggagctcg agaacttgaa cacgtagttc tcggtgaccg cataggcatc ggggtcgacg
     3661 acctccgaga gccccgcgat cgcgtggaac aggcccagca ggatcaacat gagccctgcg
     3721 aacgtggccc acccggcggc ccagtagctc ggttgccttt cggactgcat ggtccctccc
     3781 cctcgtcgga atcggccgat cagatcccca ggatctgctt cttcttcgcc tcgaactcct
     3841 cctgcgtcag gaggccctgg tccttcatcg ccgcgagctg ctggatctgg gccatgtcgt
     3901 cctgcggcgc cggagccgcg gccggtggcg gagccgcctg cggctcgggc tgcgactgct
     3961 gcgaagcggc catccttctc gacgtgcgcc gagaggtacg ccgcgcgacc ctgcgtgccg
     4021 gcatcatgcg cgttcctcct tcgtgagcct cggctacgta ccctggccag acgcggcgag
     4081 cgcctcggcc tcttccgcgg acatcaggcc gacctcgatg agatcgagcg gactgatgaa
     4141 gtgcccgccg atggggaagc cgccggcgcg aacgatcgcg tcccgcagcc cgatcgccca
     4201 gcgatgctcg agcaggatga gcgcggccgc ggcatcgttg gggatctcct ccaggacgtc
     4261 ccacgcctct tcctcgtcga acacgtcgac gccgtccgac gcggccgccg cgcccgcgat
     4321 cgctcccgcc tccatgcctt cctcgccgcc cgcgcccaga cccaggagcc cgccgacgac
     4381 ggcgccgaac tcctccgcct cgtccttcga gagttgactg cccttcagcg ccgccacgtc
     4441 accttcggca tccttgaaga cgacgagcgc gtcgatcacg cgcaccgtgt cgttctcgcg
     4501 gagccgttcg aactcgtcga tgatctcgcc gcggaagtcg ggcttgtcga accccagcac
     4561 cagcagctgc accggtccga tcgtcatgtc gtcgctcctt ccgtcgtcaa gctgtctgcg
     4621 ccgcgcccga gaagaactcg agagcggtct tggtcttcgt ctgttgctca ctcgagaggt
     4681 cgacaccctt gctgaggagc aacgccctcg cctccgccgc cacctggaac gcctcatcgg
     4741 gtcgagcctt ggtcgacagc tccacgatct gcgacccgtc cggatacatc cacagctcgc
     4801 cgaccagctt tcggccgaag ccctccgggg tgatcttcac cttgatgatc gggatcgggc
     4861 ccagggtcgt gagcgagtcg agctcgatgc cgtccggtgc gtgcgcctcg tagaacgcgc
     4921 gctgctgctt cgagaacagc ttgcggatcg gcaggacacc gctggctacc cgacggatcg
     4981 ccgtgttgtc ggccgctccc ttgaacgaag cggagcacac gaaccccccg ggcatcgcgt
     5041 ccacctcgac gttgaaggtc tccatggcgc gcagatcctc cggcaggtcg ccggggatca
     5101 ccgggcggag cttgaccacg gtatcgccct gttcgttctg acggcgtcgc gcccgaacga
     5161 ccacccccgc cgcgttcagc gacaggccgg gggtgtcgaa gaagaacacc tgacgcatct
     5221 gtgagtcgag cgggtcgacg ccgagcgccc tggcggcctc ggcccgggcg ctctcgtgga
     5281 tcgtgagctt gagctcgacg ctgtccgcgc cgcggaccag atccatcatc tggagcaggt
     5341 cccggtccga cagggccgtg gtttcggcga ccatccgctc ccctcatccg atgcgggcga
     5401 agaacgacca cccccaccct ggccctccct gatcggtccg tcgtcgtccg atagggatga
     5461 ttcgccgaac gttcgtctgc gggaggcgtc gtgctcgggc gaccccccat cgtgcgcccg
     5521 acggccgtcc agcgttgcgc ttgcgaaaaa cacgaagacg ccggaggggc taggctgctc
     5581 gcctatgggg ggtacgtcgg tccgagtccg acaggccacg cgcgggcggg gtccggagcc
     5641 cgcggcggcg aggcggcccg cgttcgatgt caccgaggcc aagctcttcc gacccccggt
     5701 ccgtccgggg atcgtgcggc gcaacgccct cgtcgatcgc ctgcgcagcg ccgaggcggc
     5761 ctccgtggtg gcgatcacgg caccagccgg gtacggcaag acgaccctgc tcgcggagtg
     5821 ggccgagcgc gaccgacgtc cgttcgcgtg ggtgtcggtg gacgaggcgg acagcgatcc
     5881 ggttgtgttc ctgacacacg tcgcggtcgc actccatcgg atcgagccga tcgagccgca
     5941 cgtgctcgag tccatcgcgt cgccaacggg atccaactcc gcccgggtgc tctcgcgtct
     6001 gagttcggcg ctcgccaggc ggacacggcc gttcgtgctc gtcctggacg acctcgaccg
     6061 cctggcggag ccattgatca cggacgcggt gtcgaggatc gccggttcca tccccgaggg
     6121 ctcggccctc gcgctcgcgg cgcgagcgct ccccgacgtc gggctccccc gcctgcgcgc
     6181 cgacggtcgg ctggaggagg tgggcgtcga cgacctcgcg ctgggcccgc cccaggcgta
     6241 tcggctgctc cgcgcggccg gcgtgcgcgc cacgaggatc gaggccgccg agctcaccga
     6301 tgcgaccgag ggatggccgg tcggcctcta cctcgccgcg ctgtccctgc aggagcagcg
     6361 gcgctccacc atgccgccga tccggttcac gggcgacgac cggttcgtcg tcgactacgt
     6421 ccgctccgag gtgctcggac ggctgtcgcg cgaacgccag cgcttcctca cccgcacgtc
     6481 gatcctggat cggctgaacg gacctctgtg cgacgcggtc atggaacggt ccggctccgc
     6541 tcgcaccctc gagtcgatcg agcggcagaa cctgctcctg gtaccgctcg atcggcagcg
     6601 agcgtggtac cggtaccaca acctcttccg cgacgtgctc cgggccgagc tcgctcggcg
     6661 cgaacccgag gcgatccccg agctgcatcg gcgtgccgcg gaccggttcg aggaagcggg
     6721 catgctcgag gaggccttcg tgcatgcgca ctcgagcggg gacgaggaac gcgcggcggg
     6781 gctcctgcag cgcgtcggcc tggccgcgta ccgagcgggc cgcatcgcca ccctccgccg
     6841 atggctcgat ctgctcggcg acgacgccat ctctcgcaac ccgggcctcg cgctcatggc
     6901 gtcgctgatc gcggcgctgg gcggcgaccc cgtcgccgcc gaacgttggt cgaaggctac
     6961 ggagggcgtg cggctcaccg ccccatcgct ccacggcacg gcctcgctcc gatcgtccga
     7021 ggcgatcgtg cacgccacca tgtctcgggg gggcacggac gcgatgttgc gggcggcgga
     7081 ggtggccgtc cgagaggaga cgggcacgag cccctaccgg gcggcggcgc tcggcctcct
     7141 cggcctcgcg acgatgatcc ggggggacga agaggtcgcc gacaccctgt tcgcagacgc
     7201 gtccgaggtg ggccttcgga tcggggggat gccgatcgcc tcactcgccc tggcggagcg
     7261 ttccctgatc gcgacctcca ggggcgacgg caccgcggcg gacgacctcg ccgagcgcgc
     7321 acgcatcgtc gtgcgcgatg cccacctcga cgagcacatc acgaccgggc cggtgcacgc
     7381 ggcctcggct cgtgcggccc tgcgagcggg cgaccacgaa gcggcgcgag cggagctcgc
     7441 agcggcccat cgcctccgcc cgatcctcac gtgggcgttc cccaccatcg cggtccagac
     7501 gcgactcgag ctcgcgcgtg tgcacgtcgg cctgtccgac gtccccggcg cccgaacgat
     7561 cctggccgag gtcgacgaga tcctcgccca ccggccggat ctcggcacgc tgacgacgca
     7621 ggcggtggag ctccgcgcgc atatcgggtc ggtgcgctcc ggacggtccc ccggacccac
     7681 gaccctgacg gccgccgagc tccgcctcct caacttcctg cccacgcacc tgtcgttccg
     7741 agagatcggc gctcgcctgt tcgtctcgcc gaacaccgtg aagacccagg cgatctcgat
     7801 ctaccgcaag ctcggagtct ccacgcgctc ggacgcggtc caggccgctc gcgacctccg
     7861 cctgctcgag ccgtaggcgc tcggaggcgc aacccgccct ttcatccggt cgggacgatg
     7921 cctcgccccg ccaccccagg gtcgaatgag cgaactgcga ggggacgacg gccatggcca
     7981 agcacgagga cgcgctccga tgcatggcgc tgggcgacga gcggtacgtc agggacgtcc
     8041 tggcgatgca gctgaccaac gtcgacgcct cgggtctgga tccgaagacc cacgcgctgg
     8101 tccggctcgg ggcgttggtc gcgctcgatg cttcgcagtc gtcgttccag tggaacgccg
     8161 cgaccgcgct cgcgtcgggc gccaccgtcg acgagatcgt cggcgtgctg atcgccgtcg
     8221 cgcagacggt ggggctggcg agggtggcct ccgccgcgcc ggcggtcgcg ctggcgctcg
     8281 ggtacgacat cgacgcggcc ctggaggccc acgacgaggg cggacggacc ctcgacatca
     8341 atagccggta atcgggatcg gtttcgacga acggagggag cgacgatgtt ggcggactac
     8401 gacttcggcg atttcttgtg gacgatgttc gcgttcttct tctggttcat gttgatctgg
     8461 atgttcatca tggtcttcgc ggacatcttt cgtcgcaagg acctgtcggg cggggcgaag
     8521 gccggttggg tcctcttgat cttcatcctg cccctgatcg gcatcctgat ctacctgatc
     8581 gcgaggccga gcatcgtcga tgcgcgcgag atgtcctcct ccgcgggaca gatgcagggt
     8641 tcggccggat acgcgagccc cgaccacttc gcctaaacgc ctggcggcgg gcgggcgtca
     8701 gttcgcggcg cgttcgagca ccgggaggat ccgctccagg ccccactcga gctcgtgctg
     8761 gctgatcacc agcggcggcg cgagccgaag ggtgctctcc tgcgtgtcct tcgccaggac
     8821 acccgcctcg agcaatcgct cggccgaaca acgcgccggt agcgcggagt cgacgagctg
     8881 gatcccggcc cacagaccgc gcccacggac ctcctcgacg gtcaggaggt ccgcggcccg
     8941 cagcgccgaa tgcaggtatg cgccgagccg cgtcgacgcc tcctggaact ccccggtccg
     9001 gagcatcgcc acgacccgac gtccgatcgc gcaagcgacg gggttgcccc cgaacgtgct
     9061 gccgtgctcc cccggcctga acaccccgag gaccgcctcg tccgcgacga ccgcggacaa
     9121 aggcacgatc ccccccgcga gcgccttgcc caggatgaac acgtccggac ggacgccgtc
     9181 gtgttcacac gcgaacgtcg tgcccgtccg ccccagcccg gtctggatct cgtcggcgac
     9241 gagcagcacg tttcgcccgg tgcagagctc gcgcgcgcgg cggaggtagc cctcgggggg
     9301 aacgacgacg ccggcctcgc cctggatcgg ctcgaccagg aaggccaccg tgtcgtcgtc
     9361 gatcgcggcc tcgagcgcgg ccgcgtcgcc gtacggcacc acctcgaacc cgggggtgag
     9421 cggcccgaag ttgcggcggg acccctcgtc tgtcgagaag ctgatgatcg tgatcgtccg
     9481 cccgtggaag ttgccgccgg cgacgatcac cttggctcgg tccggggcga cgcccttgac
     9541 ctcgtagccc cacttgcgcg cggtcttgat cgcggtctcg accgcctccg cgccggagtt
     9601 catcagcagc gccaggtcca tgccgcacag atcggtgagg tcgcgcagga acgggcccat
     9661 ctggtcgttg tggaacgcgc ggctcgtgag cgtcacccga tcgagctgct ccttggccgc
     9721 cgcgatcagc tcgggatgac ggtgtccgaa gttcagcgcc gagtacgcgg ccagcatgtc
     9781 gaggtaccgc ttgccctcga cgtcggtgac ccagcagccc tctgcctcgg caacgacgac
     9841 cgggagcggg tggtagttgt gcgcggtcgt ccgctcgacg agctcgatgt gctcctgtga
     9901 ggttcgcggc tgggccgtgc caacgtgctc ctgcgtggtc accccgatcc tccctgatcg
     9961 atccgcgcgg gtcagtctac ggacccgtcg acgccgcggc ctcgaagcag gtcgtcgagg
    10021 atctccccca tcgaacccac ggcgagggag agatgtccgt gctccggccg caggcggggt
    10081 cgagccgtcg ggatgtgctc gaccagccac tcgccatgcg cgaacggcac catcagatcc
    10141 tgccgcccct gccacaccga gaccggcacg ccgatcgacg agagctcgaa cccccagggc
    10201 cgaacgaacg cgagatcgtc gtcgaaccag ccccaaggcc cggcgcggaa cgctcggtgg
    10261 aacgacgtcg ccatgagctc gccgagcgcg ccggtgacct cggcctcgtc gacctcggag
    10321 atcacgctcc ggagctcgga cacgatctcg ttcggctcta cggcgccgag agcctccgcg
    10381 tgcggacgca tccagcggag cagctcgtcg gggtcgcgag ccgcgagcgg gtactcctgt
    10441 tggttgctct cccccatccc cgccgtccag tccagtcctt gcgcgtcgta gggcgcgacc
    10501 cccgcgatcg tcgccgcggc gcgaacgcgg tcgggcagca gcgcggcgca cgcgagcgca
    10561 tgcggcccac ctccggacca gcccatcgtg aggaagcgct cggcggcgag gtgatcggcg
    10621 agcgcggcgg cgtcggcggc cgcgctcgcc acgccgcgac cctcgtcccg gctcgagtcc
    10681 ccgaacccgg ccgcgagagc gtcaccagcc agatcccgcg ctcggcggtg gcccggtcga
    10741 acggttcgaa cggcaccgcg gcgccggggc tgccgtgatg gaagacgacc agcgtcccgt
    10801 ccggatcgcc ctcgtcgacc gcctcaaggg cgcgcccgtc gggcaacgcg atcgcgagcg
    10861 cgctgctcat acggtcacgg cggtcgcgaa tgcgtcgtac gcctcgaccg agcgcgagat
    10921 cgtcctccgt gtgcgcgatc gagatcgtcc acgggtcacc ggacgtcacg ggtcctcctg
    10981 gttcgggaac cggaggatag gccgttttca gagagcgagg tgttacgagg tgagggcccg
    11041 ggcgaaggcc tcgaagttgt cgacgtaccg ctgcgcgtcc tcgtcggtgt ggcgaaccga
    11101 cagcgtccag gtctcttgct tggaccacgg ggacttgaac acccccccgt tctgctggaa
    11161 cagccacgcg aggtatgtga tccggtcgtc gatggcgagc gagtcgcggt actcgcgaac
    11221 cggcgtggtc gagtagatca ccgatccctt ggccccgatg cccgtgacgt aggcgggcaa
    11281 ccggtagcgc tcgatcaccg cggacagacg ctccttcagg tagccgtcga cgtggttcag
    11341 cgtcccgtag gcgtccctgg tgaggacctc cgtgagcgtc gcgcgcatcg tggccatggt
    11401 gagcgggttg ccgttgaacg ttcccgccat gtcgtactcg ccgtcgacga cgagccggaa
    11461 cagctcctcg gtcgcgccga tcgcgccgca cggcgtgccg ccgccgatcg ccttggccag
    11521 acacacgatg tcgggtacga ccccgaacgc ctcgaccgcg ccgccggcgg cgagggccac
    11581 gcccgtcttc acctcgtcga aggcaaggta ggcgccgaac tcgtggcaca ggtcggcgag
    11641 cccctgcagg tacccgggct cgggcaggat gacgccacag ttcatcatcg ccggctcgac
    11701 gatcatgccc gccacttggc cggggtgctc ctcgaacccc tggcgggccg ccgccaggtc
    11761 gttgaacggc accacgacca ccaggtcggc gaacgcctgc gggatccccg gcgcctgcgg
    11821 caccgtggtg gggtggtctc gcgggcccat cagctcgggg tcggggatca cgctgaacat
    11881 cagcgagtcg tggtggccgt gataggtgcc ctcgatcttg atcaggaggt cacgaccggt
    11941 cttggcgcgc atgagtcgag cggcctccag cgtcgcctcg gtgcccgagt tgcagaaccg
    12001 ccacagcggg agcgagaagc gctcggcgag gttctccccg atcacgtcca ggtgcttggt
    12061 cggctgggcg aagtgcgtcc cgttccgcgc ctgccgttcg atcgcatcca cgatcgcggg
    12121 atgcgcgtga ccgacgacca tcgcgccgta gccgaggtgg tagtcgacgt actcgttgcc
    12181 atcgatgtcc cagatacggc tgccgtcgcc gcgctcgatg aagatcgcgt gcggcggcga
    12241 ggcctgccac gaggaggcga cgccacccgc cacgtggagc tctgcgatct cccggtaggc
    12301 gatcgaggcc cggtgcttgg gctcgagctc ggcctcctgc tcctggatca gacggtcgac
    12361 ccgctccagg tcgatctcga ccgattcgcg aaccgccatg gctcgttcct cctgcgcgac
    12421 gggctgtttg aacggccgtt caatcatggc agagcgccgc tgcctcgaca agggcccgac
    12481 gaggccgcgg gtacaatcgc cctcgaacgc ggtggccgta gctcaagtgg tcagagcacc
    12541 aggttgtggt cctgggggtt gggggttcga gtcccctcgg tcacccgaag ccccgaggtc
    12601 cggttgctag actgcgccgg gcctctagct caatctggca gagcaacgga ctcttaatcc
    12661 gcaggttcgg ggttcgaatc cccgggggcc cacaaggcaa tgtcgtgttc cggctcatcc
    12721 cggacgttcc ggcgccacct catgtttgac ggagttccgg tacatctttg acgccggtcc
    12781 cctgatctcc aggcgcttct cgtggcggat acgagcaaga cggctgctgg cgccgtcacg
    12841 tcatgagggc gtagacaact ccgagcgcgc cgagtgcccc cgaaagcagc aacgtgcgcc
    12901 gcatgctggc agcaggtgca agcttgtaga cgagcactac cgcgctcagg atgaggatcc
    12961 aggtgaggtt cgacataccg atgatcatga tcgcaaccat cagtgcggcg ctgcacccga
    13021 cacagctgag tccgtacttc acgccaacga ccaatgcact tcgcatcagg ttgaacggta
    13081 tgccatggag cgcgcacagc tcccggcacc tcgcctcgct cgcgcgtttg atcggagtca
    13141 gtgcgtagag gcccgccagc accagggcca cgaggccgac cagcccctgg ttgggcaaag
    13201 acacgggaag cgcgctgcgc acgacatagc agatgagtcc gaacgcgagc cataccgaga
    13261 gataggtgag gccgagcagg cccgtcgcgc cctgccaccc cctccttccc tccgcacttc
    13321 gggcgaactc gaacacgagc ggcagcgccg tcggcagcat cattgccgcc atcatcacga
    13381 cccaactgac ggcgaacgcg ccgggcgttc ccgggtccat tgtcattccg ctcatagatc
    13441 gagactgctc gatcgtgagc caccacgcga ccgcggccac gaccaggaag accagcgctg
    13501 tcaaggcggg gggagcgatc tcggcgcgcg tctcccccat tggggcagcc catcctcggc
    13561 acgcggggca gaatccccgc cgcctcgttg gcctgatcag cccaacggcc aagagcgccc
    13621 gactcagtga catcagtccg ggtattcgtc gcggcggtgg acccaggagc ccgccccgtc
    13681 ctcgtcttcg tcccggccct tcggaacgag gtccatgtag tggtaggcaa cgttga
//
LOCUS       DATBRK010000489         8879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1005771, whole genome shotgun sequence.
ACCESSION   DATBRK010000489 DATBRK010000000
VERSION     DATBRK010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005771"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VIH70_14265"
     CDS             <1..247
                     /locus_tag="VIH70_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117570.1"
                     /translation="PWPDPDLKPVAEGDFPGLVPIHLEHILKDRNGKAREVISALTAD
                     VAELREENKKLLDQLVAAGFRNDELAQALRADQAVRA"
     gene            244..738
                     /locus_tag="VIH70_14270"
     CDS             244..738
                     /locus_tag="VIH70_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117571.1"
                     /translation="MKRFLLVIALALGLLAASAPPAVADEHVAKPSNWWHDGTKVYTT
                     TAVNQHNQYGDLQAILKFRQRSGPNTGNFDIQIDYVRLYRVLPDRSGLLVVGENTDQN
                     LLVTETWTYVNSGWQACGPNLPIGWYYAVSRFQLIHMHTVGAYFSGWRFVRTAKWHYD
                     GRCF"
     gene            1275..1748
                     /locus_tag="VIH70_14275"
     CDS             1275..1748
                     /locus_tag="VIH70_14275"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY8117572.1"
                     /translation="MRIPSLGPRGEGWTVVQVVLMFAIIVAGVAGPVWADDVSWFRAA
                     VGIPIAIVGAALFVGGMVGIRGFLTPFPRPLDHARLQVGGAYGRVRHPIYGGLILIAL
                     GWSLISSALTLVVTVALMLVLEMKSRLEESMLAQRFPEYDAYRQRVRWRFVPGIH"
     gene            complement(2266..2547)
                     /locus_tag="VIH70_14280"
     CDS             complement(2266..2547)
                     /locus_tag="VIH70_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117573.1"
                     /translation="MELLFYIVLSAALVAEIITPAQLGPAGVRAGRSRKRALTNSSPL
                     SGFRTGESGAYGCDGGRMIAAISIGIIIIIAVMIAALAIIYFILKGSPR"
     gene            complement(2547..2885)
                     /locus_tag="VIH70_14285"
     CDS             complement(2547..2885)
                     /locus_tag="VIH70_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117574.1"
                     /translation="MAREGLISYGGVFSCRKIAGTNYWSQHAWGNALDLFPTNNKYND
                     EIARAAVTQATKRTFANRGRKLDLSNVIDHLNGRIWTPGTGWHEYNGTVGPHVHVQAA
                     PIKTGTPACA"
     gene            3257..3697
                     /locus_tag="VIH70_14290"
     CDS             3257..3697
                     /locus_tag="VIH70_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117575.1"
                     /translation="MKPFRILLIAFAVVLVGTVASAATGGVHGKPTPSPAEAVSDSPS
                     PEPTETEAPEPTETETPEATSDQTGIAPDFAACVGRTGLDNAICRHEALLKVKPDNPG
                     LTNSLAHLQANAAAKATRQAAKAAGTHGNSGTHGNSASHSPSGS"
     gene            complement(4229..4873)
                     /locus_tag="VIH70_14295"
     CDS             complement(4229..4873)
                     /locus_tag="VIH70_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEY8117576.1"
                     /translation="MVFSGVLAIWWFLWVPNWRPPLEAGERYGIDVSAHQDVIDWRQV
                     ARDGITFAYIKATEGGDFTDDRFDENWHGAQDAGLDRGAYHYFTLCTPGAEQARHFID
                     VAPPENDALPPAVDLELAGNCSTRPSPSEVKEQLGVFIRLVEEAWGREVVLYVGDDFE
                     RAYPVRHELDRPLWHRRFLLRPDLDGWLIWQIHGYARVDGIDGGVDLDVMRASS"
     gene            complement(5582..6331)
                     /locus_tag="VIH70_14300"
     CDS             complement(5582..6331)
                     /locus_tag="VIH70_14300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8117577.1"
                     /translation="MPDEYTDATYGDRVAEAYDEWYEASFAEDTVAAVSFLRELAADG
                     PALELGIGTGRLALPLAEAGIDVHGIDASEAMVGKLKSKPGGHRIPITIGSFANFSLE
                     TRFRLVYVVFNTFFGLLTQDEQISCFAAVARHLTPDGVFAMQAFVPDVTRFDVHNQRV
                     AADSVGIDEVALETSTHDPFTQRTDSAHVVIRDGSIRMYPVRIRYAYVSELDLMGRLA
                     GLRLRERWAGWDREPYPSRRWTHVSVWEKGN"
     gene            complement(6480..6878)
                     /locus_tag="VIH70_14305"
     CDS             complement(6480..6878)
                     /locus_tag="VIH70_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8117578.1"
                     /translation="MHPEIDGIRAIPLFGQLSPHELETVASWLEIREESEGRRLTPEG
                     VSGYEFFMIEEGTADVVHGGDVIASLGPGDFFDEMAMMGDGRRVADVVARSPITLFEM
                     FGTHFRELEMHMPRVAATISAKLEERRSAL"
     gene            7338..>8879
                     /locus_tag="VIH70_14310"
     CDS             7338..>8879
                     /locus_tag="VIH70_14310"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEY8117579.1"
                     /translation="MRRWLVVLLATLLVVAGGGSAAAFFLMRGAGEELLQLVPASSEV
                     VVTAYLDPSAGQKVNLMALARRFPALRDDERIRKQVDVVLDEALEGSGLSHEDVRPWL
                     GSQVAIVVDLGANADQPTVSFLVASKDDDAAQATLEKALGGSLGAEQSSEYRAEQSSE
                     YRGVTMHVFDGGTSFVGYVIVHHVVVLSNQSIGLTRVVDVSEGTAQAMADDPAFLDTI
                     SSLPEGKLGLAYVNPTEIVNRAFSATGLGAAGTAPGLDQLRAIRGVAATLSAHPDGLA
                     FDVAVRLDPSMLDPATRLQLDRPAQENGLLGFVPADAYAVATQQGLDETVKQTVDQLL
                     TTPQGELIRQRIGVDDTLAALTGDLAFEVGPGSGPVPAAGAIVIGVRDASAVQRTLDG
                     LADLVLAAQGRPGAIRPAFGDPSGPTHHDVLGAVPEATWRTSTYQGTTIRYLDDPKLS
                     GMGFVPAYAVVEGAAIVGSSPTEVRKVIDTGAGSEPNIGASSSYGRAVTRVPDEAGTW
                     YVDVAA"
BASE COUNT         1509 a         2940 c         2932 g         1498 t
ORIGIN      
        1 tccctggccc gatcccgacc ttaagcccgt ggcggagggc gacttcccag gcttagtgcc
       61 gattcacttg gagcacattc tcaaggaccg gaacggaaag gctcgcgagg tcatcagcgc
      121 gctcacagct gacgtcgcgg agctgcggga ggagaacaag aagctgctcg accaactcgt
      181 cgcggcgggg ttccgcaacg acgagctcgc gcaggcgctc cgagccgacc aggcggtgag
      241 ggcatgaaga gattccttct cgtcatcgct ctcgcgttgg ggttgttggc cgcaagcgcc
      301 ccgcctgcgg tcgcggacga gcacgtggcc aagccgtcca actggtggca cgacgggacc
      361 aaggtctaca ccacgactgc ggtcaaccag cacaaccagt acggcgatct acaggcaatc
      421 ctgaagttcc gccagcgctc tggcccgaac accggtaact tcgacattca gatcgactac
      481 gtccgacttt atcgggtgct gcctgatcgg tccggtctcc tggtggtagg ggaaaacaca
      541 gaccagaatc tgctcgtcac cgagacctgg acgtacgtga acagtgggtg gcaagcgtgc
      601 ggtccgaacc ttccgatcgg ctggtactac gccgtctccc ggttccagct catccacatg
      661 cacaccgtcg gtgcctattt ctccggctgg aggttcgtgc gcacggccaa atggcattac
      721 gacgggcgct gcttctagaa tctcccgaat ccgtgggttg tgggttcaag tcctacccgg
      781 agcgctcgtg ttggcgagct aagcgtcggc tccccgtgcg gcgcgggcac gttacataat
      841 ggcgagcatg agcaaggaag gcggctcaac ccccgagtac ccagacatcc caccgccata
      901 cccgagctac taccgccgct actacgccgg tggccctccg cctgagcact gagcggcgtc
      961 gagactcgga cggggacgtt caggagcgtc gccccggcct cggcctctgg tcgcgtctca
     1021 aggcttggtt cgcccgcccg gctccgaagc gcgacagcta cgtctcgaac gtggtgaatc
     1081 ccgacgaccg ccggttcatc ccaggtcgct gaagcttccc ttcaagaact ccgccttctc
     1141 gttcccctcc cgcagcatcc cgacgagcac cgctcggagc gtccggaccc tcgcggtcga
     1201 tcagcaactc ggcgaggata catattgatg ccgggcgagt gggaactgtt cgaggcgtcg
     1261 atcggatagt cgggatgcgg atcccctctc tcggtcctcg cggcgagggg tggaccgtcg
     1321 tccaggtcgt gctcatgttc gcgatcatcg tggccggggt cgcaggacca gtgtgggcgg
     1381 acgacgtttc gtggttccga gccgccgtcg ggatcccgat cgcgatcgtt ggcgcggccc
     1441 tttttgttgg aggcatggta gggatccgtg gcttccttac gccgttcccg aggccgctcg
     1501 accacgcacg gctacaggtg ggtggcgctt acgggcgcgt acggcatccg atctacggcg
     1561 ggttgatcct catcgccctc gggtggtccc tcatctcctc agcgctgacg ctcgtagtca
     1621 ccgtcgctct gatgctcgtg ctcgagatga agtcgcggct cgaggagtcg atgctcgcgc
     1681 agcgctttcc ggagtacgac gcgtaccgac agcgggtccg ctggcgattc gttccgggga
     1741 tccattgacg ggctcggctc gggacgccgt ccagatgtag gcgtcggagc gatggtgctc
     1801 aaaatcggcg tctgcatgga tagctccgct ccagacctac accgccggct cgccgtgagg
     1861 cgcgggctcc acgtggccgg cggcaacgac tcgacttctc acggtttcgt gtgaggggat
     1921 cggccatctc gagcgttcgt cgcgtgcttc agcgagcggc ggttgccctc gccacgccac
     1981 cccattgcga ggaggacatc agcctgctcc catcagaaac ccctggatga gactcggcca
     2041 tctccgagca ggcggtgaaa agcgcgaccg cgtagcctcc ggatcgaatg cgtccggatg
     2101 aagtcgagcg ccgactacgg gagcgtctcg acgccctcgg ccccgctccc ccgccgagct
     2161 gctccacgtc ctgatgctcc ccgactttga ggagcggacc ggatcggcga gtactggcgg
     2221 aacccaggca tccgcgtgga gcgaggcgag gcccagtgct tgcggctacc gaggcgatcc
     2281 cttcagaatg aagtagatga tcgcgagcgc agcgatcatt acggcgatga tgatgatgat
     2341 cccgatgctg atggcagcga tcatccgacc accatcgcaa ccataagccc ccgactcgcc
     2401 tgtgcgaaac cctgacaagg ggctggagtt ggtcaaggca cgcttccgcg acctgccagc
     2461 tcgaacgccg gctgggccca gttgagctgg ggtgatgatc tcggccacca gggctgcgga
     2521 tagcacgatg tagaacagca gctccatcac gcacaggcag gggtaccggt cttgatgggg
     2581 gcagcctgga cgtggacgtg ggggccgacc gtgccgttgt actcgtgcca gccggtgccc
     2641 ggggtccaga tgcgcccatt gaggtggtcg atgacgttgg atagatcgag cttgcgcccc
     2701 cggttggcga aggtgcgctt cgtagcctgg gtaaccgcgg cccgcgcgat ctcatcgttg
     2761 tacttgttgt ttgtggggaa cagatccagc gcgttgcccc aggcatgctg gctccagtag
     2821 ttcgtgcccg cgatcttgcg gcagctaaag acccccccat aggagatgag gccctccctg
     2881 gccactcgct gcatgacacg gcagacccgc tcgcaggagg gggtaatcac ggacgtggag
     2941 ttgatcggaa atgtcaaaag tgccctcctt gcggggcaag tcttacatgc cggaatggga
     3001 gggagggctg gaccacccac ccgggcctag cgttgccctg actgggaggc ggaccggacg
     3061 ctccgggcgg tgctcgtcgg caagttgcga gagaccgacc gcgatcggtg aagggccggg
     3121 ctgagagtcg tcccccgggg cggccgcctg aactagccgt tcggccaatc gcgttcgcga
     3181 ggccgtccgg ccgataccgc cgaaaccagc ccttcgaccg atcgtggtcg cgggcggtcc
     3241 agccgatact gagcccatga agccgttcag gatcctcctc atcgcgtttg ccgtggtcct
     3301 cgtgggcacg gtcgccagcg ccgccaccgg cggcgtgcat ggcaagccca caccaagccc
     3361 cgcggaggcc gtctctgact cgccatcacc ggagcccacg gagaccgagg cccccgagcc
     3421 caccgagacc gagacccccg aggcgacctc ggaccagacc ggtatcgcac cggacttcgc
     3481 cgcctgcgtc ggccggaccg ggctcgacaa cgcgatctgc cgtcacgagg cgctgctcaa
     3541 ggtgaagccg gacaacccgg gccttaccaa ctcgctcgcg cacctgcagg cgaacgcggc
     3601 ggcgaaggcc accagacagg ccgcgaaggc tgcaggcacg cacgggaact ctggcacgca
     3661 cgggaactcc gcgtcgcaca gcccgtcggg ttcgtagcgg agcgcggatc gagttggcga
     3721 agaggccggg aaccccccgc cccggcctct cgctctctat cggtagaccg tccctcgact
     3781 acgaggtcta ccccaagccc tcgtgatgtg gggcgcgggc tgtcccccag gagtcatgaa
     3841 aggagtgaga gggagcgccg tcgcgcccgg ccgtgacctt gagccggtgc atagggtgca
     3901 tcggcagtga cggcctgccg cttgagagca ggtccctcgc caagctgctg atcgactgcg
     3961 aggaggatcg gacgctgcgg gcggtgttgt cgggatgctg aggaaaacga gctgagtctt
     4021 ctcggtcact ggggcaccgg gagcccgagc cgcacggcga gccgggctgg ggtgctggat
     4081 ctcgatccaa caggcttgag caaccggcgg ggcatcactc cgaccgcccc cgccccacgc
     4141 ggctcctcgt ctccccccga ccagttcact cgtcaggggc ggcgcaccaa gagccgactg
     4201 gtctcccgcc ttcgcgtaga ccgggggctc aggacgatgc gcgcatcacg tcgaggtcaa
     4261 cgccgccgtc gatgccgtca actcgagcgt agccgtggat ctgccagatg agccatccgt
     4321 cgaggtccgg acgaagaagg aaccggcggt gccacagggg gcgatcgagt tcgtggcgga
     4381 cggggtacgc tcgctcgaag tcgtcgccga cgtacaggac gacctcgcgt ccccaggctt
     4441 cctcgacgag gcggatgaag acaccgagct gttccttgac ctccgacgga ctgggtcggg
     4501 tactgcaatt accggccagc tcgaggtcaa cggcgggcgg aagggcatcg ttctcgggag
     4561 gagcgacgtc gatgaaatgg cgagcttgct cggcgccggg cgtgcacagg gtgaagtagt
     4621 ggtaggcacc tcgatccaat cccgcgtctt gggctccgtg ccagttctca tcgaagcggt
     4681 cgtcggtgaa gtcgccgccc tcggtggcct tgatgtacgc gaacgtgatg ccgtcgcgcg
     4741 cgacctgccg ccaatcgatg acgtcttgat gagcggacac gtcgatgcca tatcgctcgc
     4801 ctgcctccag aggcggccgc caattcggca cccacaggaa ccaccaaatg gcgagcaccc
     4861 cggagaaaac cacgacggcg agaatcccaa tgaggggcca acgtcgacgt cgccgagagt
     4921 gccttggcgt gtcgccttgg tcgggcatgg ttgactgagg catgccttcc atagtgtcat
     4981 caacacgtcg gctgggtgcc tcaccatcaa ccctcgctcg ttgaccggct tagccaccga
     5041 gtgctgatcc acgaacacac atcgcccatg agcgaccggt ccatcgcagc agtgatcttg
     5101 cgacgacttg ttgagtggcg atccggcttc tgaaactgac cgatgtgtgt cagccctggg
     5161 tctcgagact gtcggccatc tcccggactc tcgccgattc caacggatgg tctcgtgcgg
     5221 gactcgcccc ggattttcag ctgcttggtt gctccacacc taggctctgg cctgctcacg
     5281 agcggagcga ttggtcgatt ccctgaggtg aggcccgcaa gggcttggct cctcactcgc
     5341 ttggaagggc gttcgaggaa atgagctacg acatacacgt ctccggggct tctccgccga
     5401 gggccattag acaacgcgag ccagacatga ccggggaagg acgaggacga accaaactga
     5461 tttcttggcc cctaaccctc ctgaacgccc gacaacagtc ctgactcgta cacgtggaag
     5521 ggcggcgacg cggccccgac ttgccgggtg gtcatttcgt ccttctgtct acccggtcga
     5581 actagttgcc cttctcccac acggacacgt gggtccacct ccgcgacgga tacggttctc
     5641 ggtcccatcc ggcccagcgc tcgcgcagcc tgagaccggc taggcggccc atcagatcga
     5701 gttcggacac atacgcgtag cggatcctca ccgggtacat acggatgctt ccgtcgcgaa
     5761 tcaccacgtg ggcgctgtcg gtccgttgag tgaacgggtc gtgagtcgag gtctcgaggg
     5821 cgacctcgtc gatacccaca gagtccgcgg ctacacgctg gttgtggacg tcgaaccgcg
     5881 tgacgtctgg cacgaacgcc tgcatcgcga agacgccgtc gggtgtgagg tgccgtgcca
     5941 ccgccgcgaa gcaggagatc tgctcgtcct gcgtgaggag cccgaagaac gtgttgaata
     6001 cgacatagac gagccggaac cgagtctcga gcgagaagtt cgcaaagctc ccgatcgtga
     6061 tcgggatgcg atgacctcca ggcttggact tcagcttgcc gaccatcgcc tcggatgcat
     6121 ctattccatg cacgtcgatt cccgcttcgg ccagcgggag cgccaggcgt ccggtcccga
     6181 tccccagttc gagcgccggg ccatccgcag ccaactctct caagaacgag accgcggcga
     6241 cggtgtcctc cgcgaacgat gcttcgtacc actcgtcgta cgcctcagcg acccgatcgc
     6301 cgtaggtagc gtcggtgtac tcgtccggca tgtgcggagc caagcaccag ggaagacgcc
     6361 ggtcaaacgc agaaggacca actcgatgct cgtcgcacat ccaccaatgc cactgagccg
     6421 gcccatatgg agcggggccg ctcggatggt cgatgctcga gcctcggagc atggccggct
     6481 cagagcgccg accgtcgctc ctcgagcttc gcggagatgg tggcggcaac ccgcggcatg
     6541 tgcatctcga gctcgcggaa gtgcgtcccg aacatctcga acagcgtgat cggcgacctc
     6601 gcgacgacgt cggcgacccg gcgcccgtcg cccatcatcg ccatctcgtc gaagaagtcg
     6661 ccgggcccga gcgacgcgat gacgtcgccg ccatgcacga catccgccgt cccctcctcg
     6721 atcatgaaga actcgtaacc ggagacgcct tcgggcgtga gacgcctgcc ctcgctctcc
     6781 tctctgatct cgagccagga ggccacggtc tccagttcgt gtgggctcag ctggccgaac
     6841 agcgggatgg cgcggatccc gtcgatctcg ggatgcacgg tcgctccttc ggtcgtagat
     6901 gcgggcgcat cccagatggt ggcgggcctc gtctgccgtc gccgtcacga cgtcttcgca
     6961 gatcgttcac ggatccgccg catcgcgcga ggagcgaggc ccgacattgg acctcacccg
     7021 gtacgcaggc tcatggcggg ttcggagttc gacaggttgc ccgacctcgc tgaactcggg
     7081 gccccgaccg gccgataccg attctgaccc cggagccgct gctcagggag gatttcaccg
     7141 aagccccgag gagtccgttc atggatccga acagcccggg atcacccggc cgagagcctg
     7201 aaccgcacat atcgctgccg gtcccaccga cgaatgaggt cgtgccgccc agcgggggtg
     7261 cgggtccgac cccaccaccc gggtcgtcct ggggaccctg gtccgagggc cgcccgcccg
     7321 cgcccgctac ccgccgcatg cgacggtggc tggtcgtcct gctcgcgacg ctgctggtcg
     7381 tagccggtgg cgggtctgcg gccgccttct tcctgatgcg tggggcgggc gaggagctgc
     7441 tccagctcgt cccggcgtcg agcgaggtcg tcgtcaccgc ctacctcgat ccgtcggccg
     7501 gccagaaggt gaacctgatg gcgctcgcgc gccgattccc ggcgctgcgc gacgatgagc
     7561 ggatccggaa gcaggtcgat gtggtgctcg acgaggcgct cgagggctct ggcctgagtc
     7621 atgaggacgt tcggccgtgg ctcggttcgc aggtggcgat cgtcgtggac ctgggggcga
     7681 acgccgacca accgacggtc tccttcctcg tcgcgagcaa ggacgacgac gccgcacaag
     7741 cgaccctcga gaaggctctg gggggctcgc tcggcgccga gcaatccagc gagtaccgag
     7801 ccgagcaatc cagcgagtac cgaggggtaa cgatgcacgt cttcgacggc ggtacgtcgt
     7861 tcgtcgggta tgtgatcgtt caccacgtcg tcgtgctcag caaccaatcg ataggactca
     7921 cccgcgtggt cgacgtgtcg gagggaaccg cgcaggccat ggccgacgat ccggcgttcc
     7981 tggacacgat ctcgtcgttg ccagagggca agctcggact cgcgtacgtg aacccgaccg
     8041 agatcgtgaa ccgggccttc tcggccaccg gacttggtgc ggcgggcacg gctcccggcc
     8101 tcgatcagct gcgcgcgatc cgcggcgtcg cggcgacgct gtcggctcat cccgacggcc
     8161 tggcgttcga tgtcgcggtg cgactggatc catcgatgct cgatccggcg acacgtctgc
     8221 agctcgaccg acccgctcag gagaacgggc tgctcgggtt cgtcccggcg gacgcgtacg
     8281 ccgtggcgac gcagcagggt ctcgacgaga ccgtgaagca gaccgtggac cagcttctca
     8341 ccactcccca aggggagctg atccgtcagc ggatcggcgt ggacgacacg ttggccgcgc
     8401 tcaccggtga tctcgcgttc gaggtcggtc ccggatcggg tcccgtgccg gccgcgggcg
     8461 ccatcgtgat cggcgtgcgc gatgcctccg cggtccagcg cacgctggac gggctcgccg
     8521 acctggtgct cgccgcgcag ggcagacctg gggcgatccg ccccgcgttc ggcgatccgt
     8581 ccggaccgac gcatcacgac gtcctcgggg cggtcccgga ggcgacgtgg aggacctcga
     8641 cgtaccaggg cacgacgatc cggtacctgg acgatccgaa actctcgggc atggggttcg
     8701 tgccggccta cgcggtggtc gaaggtgccg cgatcgtggg atccagcccc accgaggtcc
     8761 ggaaggtgat cgacaccgga gcggggagcg agccgaacat cggcgcgtcg tcctcgtacg
     8821 ggcgggccgt cactcgggta ccggacgagg cgggcacgtg gtacgtggac gtggcggcg
//
LOCUS       DATBRK010000490         4463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1007735, whole genome shotgun sequence.
ACCESSION   DATBRK010000490 DATBRK010000000
VERSION     DATBRK010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007735"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VIH70_14315"
     CDS             <1..709
                     /locus_tag="VIH70_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117580.1"
                     /translation="PGPERDHRYGSVAMQITPRDKRILQIAGVVVPLLLLVYFFVLRP
                     DGDEDLALPSGLTGITGSTGAPTESPSVTPSPTPRETLPPVSLAGSRDPFSIPPGLET
                     STGGSVSPTTTAATGTTTTIPATTTFPPPPPPPPTTTTSTFPPPPPPTTTTTPPPGGG
                     GGGGEHPGNKILVGGHNVKLIGVAGGGRKLDVAVDGKVYTVQPGATFDDNFKLVKIDG
                     KCATFLFGDQSFELCEK"
     gene            950..2101
                     /gene="aroC"
                     /locus_tag="VIH70_14320"
     CDS             950..2101
                     /gene="aroC"
                     /locus_tag="VIH70_14320"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY8117581.1"
                     /translation="MLRLLTAGESHGKALVGVLEGLPAGIPVSPKEIATELARRRHGH
                     GRSGRQKLEGDRFEIVSGVRHGLTLGSPIGVTIENAEWDVKYRDLMSVEGEADPAERL
                     TRPRPGHADLAGAQKYGFDDVRNVLERASARETAMRVALGVFCKALLAELGIAVVSHV
                     VRIGKVAVRSTRVPRPEDLETIDASPVRCLDEVIADRMVAEIDRARKARDTVGGVFEV
                     IAFGCPPGLGSHVQYDRKLDARLALSLMSIQSVKGVEVGDGFATASKPGSRAHDEIVT
                     GRGGLRRKSSRSGGIEGGISTGQPIRVRAAMKPFSTVPKPLATVDLATGRPEVAITQR
                     TDVCAVPAGAVVGEATIAFVLAQAVLEKFGGDALPETRRNLDAFVAGLR"
     gene            2130..2630
                     /locus_tag="VIH70_14325"
     CDS             2130..2630
                     /locus_tag="VIH70_14325"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY8117582.1"
                     /translation="MIVYLVGMPGSGKSTVGPELARRLGVPFVELDERVEHAAGSSVR
                     EIFERDGEARFRELEAAALVEAASNDPSVVSCGGGVVLEPANRVSLRATGEVVFLSVP
                     LEVLAERVRPGADRPLIRSEGDLERVFRDREPLYREFAAHVVDGSGPVDDVAAAIEKE
                     LLRWSA"
     gene            2618..3721
                     /gene="aroB"
                     /locus_tag="VIH70_14330"
     CDS             2618..3721
                     /gene="aroB"
                     /locus_tag="VIH70_14330"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY8117583.1"
                     /translation="MERVSVRIPGRAYEVCVGSGVLALFDELVPALAGAERAFVIADA
                     PVAGRYLATLSDGLAARGLGVVHLGVPEGEGAKSLQVMNALERQLATQGAHRDDPIVA
                     LGGGAVGDLAGFVAATYMRGVPFVQVPTTLTAQVDAAIGGKTGVNIPEGKNLVGAFHQ
                     PIGVLADVETLATQSDRAFRSGLAEVAKYALTLDPELLETLERDPSRLLEREPALLEH
                     VVTRCVRAKAAVVAVDERDEGRRLVLNYGHTLGHALERLDEFAGRTHGEAISAGMVFA
                     ARLSEALGRGPSGLAARHVRLLASLGLETDGGLPPTEEVLSAIGMDKKYRGGSRFVLL
                     EDVGRPFVQDAVPQELVRATLDLSGSGAQAMGG"
     gene            3724..4158
                     /locus_tag="VIH70_14335"
     CDS             3724..4158
                     /locus_tag="VIH70_14335"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY8117584.1"
                     /translation="MRVLFLFGPNLGALGRREPERYGTQTLEEIMDEVAQRGARLGHE
                     LAWRQTDHEGELIGWLNGAAAEGFQVVVANPGALSHTSYALRDAVEGCGLPVVEVHMT
                     NIYAREEFRRHSVISAACRATIAGAGAGGYHVALEALPWITG"
     gene            4143..>4463
                     /locus_tag="VIH70_14340"
     CDS             4143..>4463
                     /locus_tag="VIH70_14340"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEY8117585.1"
                     /translation="MDHGLRRSRLGERLVGLEAEGLIVTRLPNVRYLTGFTGSNAQVV
                     VGADGSAVFLTDGRYGAQSSHEVPDLPRTVYQQGYPQQLVDACRTLGIGRAGFEGHGL
                     TYEAW"
BASE COUNT          621 a         1533 c         1674 g          635 t
ORIGIN      
        1 tcccgggccc gagcgagatc accggtacgg gagcgtagcc atgcagatca ccccccgaga
       61 caagaggatc ctgcagatcg cgggggtcgt ggtgcccctg ctgctgctcg tgtacttctt
      121 cgtgctgcga ccggacggcg acgaagatct cgcgcttccc agcggcctca cgggcatcac
      181 cgggagcacc ggtgcgccca cggagtcgcc gagcgtgacg ccgtccccga cgccccgcga
      241 gacgctgccg cccgtgtcgc tggccggatc aagggatccc ttctcgatcc cacccggtct
      301 cgagacgtcc acgggcggca gcgtcagccc cacgacgacg gcggcgaccg gcacgacgac
      361 gacgatcccg gcgacgacga cgttcccgcc gccaccgcca ccgcccccga ccacgaccac
      421 gtcgaccttc ccgccgccac cgcccccgac cacgaccacc acgcctccgc ccgggggcgg
      481 gggaggcggt ggcgagcacc cggggaacaa gatcctggtc ggcgggcaca acgtgaagct
      541 gatcggtgtg gccggcgggg ggaggaagct cgacgtggcg gtcgacggca aggtctacac
      601 ggtgcagccg ggggcaacgt tcgacgacaa cttcaagctc gtgaagatcg acgggaagtg
      661 cgccacgttc ctgttcggcg atcagagctt cgagctctgc gagaagtagg accctcggga
      721 cgccggcgcc gtgcgccggt gttcgggttc gagccgacgg gtcggggagc gctgccggag
      781 gcaccggcgc cgcgcacggg ggagcggccg ccggtccgag agggcggcct cggtgacggg
      841 gccgccctcc acccttcctc gacatcccgg gccatcccgc ggggcccgga gccgttccac
      901 cccggtccga acggcgggtg ccgaggaagc ggtctcctat gatgaaccca tgttgcgcct
      961 gctcacggcc ggtgaatcac acgggaaggc gctcgtcggt gtgctcgagg gcctgcctgc
     1021 cggcatcccg gtctcgccca aggagatcgc gaccgagctc gcgcggcggc gtcacggcca
     1081 cgggcggagc gggcgacaga agctggaggg cgaccggttc gagatcgtgt ccggagtccg
     1141 gcacggcctc acgctgggca gtccgatcgg cgtcacgatc gagaacgcgg agtgggacgt
     1201 gaagtaccgg gacctgatgt ccgtcgaggg cgaggcggat ccggcggaac gcctgacgcg
     1261 gcctcggccc ggacacgccg acctggccgg cgcccagaag tacgggttcg atgacgtccg
     1321 caacgtcctg gagcgcgcga gcgcacgcga gacggccatg cgggtcgcgc tgggcgtgtt
     1381 ctgcaaggcc ctgctcgccg agctcgggat cgcggtggtc tcccacgtcg tgcggatcgg
     1441 caaggtcgcg gtgcgctcga cacgcgtgcc gcgccccgag gacctcgaga cgatcgacgc
     1501 gtcgccggtc cgatgcctcg acgaggtgat cgctgatcgg atggtggccg agatcgatcg
     1561 cgcccggaag gcccgcgaca ccgtcggggg ggtgttcgag gtgatcgcgt tcgggtgtcc
     1621 acctgggctc gggtcccacg tccagtacga ccgcaaactc gatgcgcgtc tcgccctgtc
     1681 gctgatgagc atccagtcgg tcaagggcgt cgaggtgggg gacggtttcg ccacggcgtc
     1741 caagccgggc tcgcgcgccc acgacgagat cgtgaccggc cgcggcgggc tgcggcggaa
     1801 gagctcgcgg tcgggcggga tcgagggagg catcagcacg ggccagccga tccgcgttcg
     1861 ggcggcgatg aagccgttct ccaccgtgcc gaaaccactg gcgaccgtcg atctggccac
     1921 gggaagaccg gaggtggcga tcacgcagcg gacggatgtc tgcgccgtcc ccgcgggtgc
     1981 ggtggtcggc gaggcgacga tcgcgttcgt gctggcgcag gcggtcctgg agaagttcgg
     2041 tggcgacgcc cttcccgaga cgcgccgcaa tctcgacgcg ttcgtggctg ggctgcggtg
     2101 accgccacac gcggcgtggt gggtcgatcg tgatcgtcta tctcgtcggg atgccggggt
     2161 cgggcaagtc gaccgtgggt cccgagctcg cgcggcggct cggcgtcccc ttcgtcgagc
     2221 tcgacgagcg cgtcgagcac gccgcgggct cctcggtccg cgagatcttc gagcgcgacg
     2281 gcgaggctcg cttccgcgag ctcgaggcgg ccgccctcgt cgaggccgcg tcgaacgacc
     2341 cgtcggtcgt ctcgtgcggc ggcggggtcg tgctcgaacc ggccaaccgg gtctcgcttc
     2401 gggcgaccgg cgaggtggtg ttcctctcgg tcccgctcga ggtgctcgcg gagcgggtgc
     2461 ggcccggggc cgaccgtccc ctgatccggt ccgagggcga cctcgagcgc gtgttccgtg
     2521 accgagagcc gctctaccgt gagttcgccg cccacgtcgt cgatgggagc gggcccgtgg
     2581 acgacgtggc cgcggcgatc gagaaggagc tccttcgatg gagcgcgtga gcgttcgtat
     2641 ccccgggcgc gcgtacgagg tctgcgtcgg gtcgggcgtg ctggcgctct tcgacgagct
     2701 cgtcccggcg ctcgcgggcg ccgagcgcgc gttcgtgatc gccgacgctc ccgtcgccgg
     2761 gcgctacctc gcgaccctgt cggacggtct cgccgcccgc ggcctgggcg tggtgcacct
     2821 cggcgtgccc gagggcgagg gggccaagtc gctgcaggtc atgaacgcgc tcgaacgcca
     2881 gctcgccaca caaggggcgc acagggacga cccgatcgtc gcgctcgggg gcggcgcggt
     2941 cggggatctc gccgggttcg tcgcggccac gtacatgcgc ggcgtccctt tcgtgcaggt
     3001 gccgacgacg ctcaccgcgc aggtggacgc ggcgatcggc ggcaagaccg gcgtcaacat
     3061 ccccgagggc aagaacctcg tgggcgcctt ccatcaaccc atcggcgtgc tggccgacgt
     3121 ggagacgctc gccacgcagt ccgatcgagc attccggagc gggctcgcgg aggtggcgaa
     3181 gtacgcgctc accctcgatc ccgagctgct cgagacgctc gaacgcgatc catcgcgcct
     3241 gctcgagcga gaaccggcgc tcctcgagca cgtggtgacc cgatgcgtgc gcgccaaggc
     3301 cgcggtggtc gcggtggacg agcgcgacga aggccgccgc ctggtcctga actacggcca
     3361 cacgctcggc catgcgctcg agcgcctcga cgagttcgcc ggccggaccc acggcgaggc
     3421 gatctccgcg gggatggtat tcgcggccag gctctcggag gcgctcggcc gtggcccgtc
     3481 gggactcgcg gcccggcacg ttcggctgct cgcgtccctt gggctcgaga ccgacggcgg
     3541 gctcccgcct accgaggagg tcctctcggc catcgggatg gacaagaagt accgcggcgg
     3601 gtcgcgcttc gtcttgttgg aggacgtcgg ccgcccgttc gtgcaggatg cggtcccgca
     3661 ggagttggtg cgcgccacgc tcgacctgtc cggctcgggc gcgcaggcga tggggggttg
     3721 agggtgcggg tcctgttcct gttcggcccg aacctcgggg cactcggacg ccgggagccc
     3781 gaacggtacg gcacgcagac gctggaggag atcatggacg aggtggccca acgcggggcc
     3841 cggctcggcc acgagctcgc gtggcgacag accgatcacg agggcgagct gatcgggtgg
     3901 ctgaacggcg ccgcggccga agggttccag gtcgtcgtcg cgaaccccgg cgcgctctcg
     3961 cacacctcct acgcccttcg cgacgcggtc gagggctgcg ggttgccggt ggtcgaggtc
     4021 cacatgacga acatctacgc ccgcgaggag ttccgccggc actccgtgat ctcggcggcg
     4081 tgccgcgcaa ccatcgcggg tgcgggtgcg ggcggctacc acgtcgcgct ggaggcgctc
     4141 ccatggatca cgggctgaga cgctcgcgcc tcggcgaacg cctcgtcggg ctcgaggccg
     4201 aggggctgat cgtcacccgg ctacccaacg ttcggtacct cacggggttc acggggtcca
     4261 acgcgcaggt cgtggtcggg gcggacggct cggcggtgtt cctgaccgac ggccgatacg
     4321 gtgcgcagtc gagtcacgag gtgcccgacc tgccgcggac cgtctaccag cagggatacc
     4381 cgcaacaact ggtcgatgcc tgtcggaccc tcgggatcgg tcgggcgggg ttcgaaggcc
     4441 acgggctcac ctacgaggcg tgg
//
LOCUS       DATBRK010000491         6629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U4127
            k141_1010788, whole genome shotgun sequence.
ACCESSION   DATBRK010000491 DATBRK010000000
VERSION     DATBRK010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959027
            Sequence Read Archive: SRR11673296
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 12:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010788"
                     /isolate="SMAG_U4127"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1866 N 2.2146 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VIH70_14345"
     CDS             complement(<1..334)
                     /locus_tag="VIH70_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117586.1"
                     /translation="MQWIALPAGIVILLWTLLDVFRTLVMPRAARGRSRLSRILFRPL
                     WRPWRWVGVRRKTIQARERVLAGAAPFFFFVLLGAWVSLALLGYALILWSPAFVDGIE
                     RGGDSFLDA"
     gene            complement(424..921)
                     /locus_tag="VIH70_14350"
     CDS             complement(424..921)
                     /locus_tag="VIH70_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117587.1"
                     /translation="MIHRERVRVWSIAFRSWSAFGGPLMPGGETADTVESPGASTDPS
                     GQGGEPPKLASFFRRRMLFGVLGVVLVVGAAAVASRSDAEVTVPRLIGTRVDPQLDAL
                     RLRLEAADLALGDVSVGACPEYDIPGASLEQPPGTIIEQHPSAGSSVVTSTAVDVTVC
                     LPETG"
     gene            complement(948..1766)
                     /locus_tag="VIH70_14355"
     CDS             complement(948..1766)
                     /locus_tag="VIH70_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY8117588.1"
                     /translation="MSTWQTIALGAVAGFTIFLGLPLGRLKGGSLGLKTFLSGLSAGI
                     LVFLLFDVLAHATEPLEEAVVDGAWGELFAVGLVYVAGLGVGLLSLPYFFRLTLHRAR
                     GASLGPGAMAVAEIGLTPDRQALRLGMTIAVGIGLHNFSEGLAIGQAANANEISLAVL
                     LVVGFALHNATEGFGIVGPLAGAGVQATWGWLLVAGLVAGGPTFLGTIVGTSFSSEYV
                     FVGFLALAAGAILYVIGELFASGRRLSWILTLWGVLGGFLLGLATDLVIEAAGG"
     gene            1887..2651
                     /locus_tag="VIH70_14360"
     CDS             1887..2651
                     /locus_tag="VIH70_14360"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEY8117589.1"
                     /translation="MARPAAPAPKPSAPLERAVRSETVKHYAETIFYIRQEEGRVRPG
                     RLAEWLGVSAPTVTVTLQRLARDGFVEIAGDRSVTLTPTGEDLAAKIVRVHRLLERWL
                     TDVLGLDWAAADEEAQRLASGVSELVADRLDETLGYPATCPHGNVVPGREPPYGELVA
                     LSDLQPGVGARVRRISEVAEHDAPDLLRELDAYGLVTGARIEVSGADASVQAIPVDVG
                     GVTRPIGTNVGRLIWVEPESSGKGSTAKPRRAPSDR"
     gene            2992..3420
                     /locus_tag="VIH70_14365"
     CDS             2992..3420
                     /locus_tag="VIH70_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8117590.1"
                     /translation="MQVQGRRSAEGGGRCLDLEMPSLSGALTDLRSAFAELPVPASAL
                     DDARLLVSELVANSMRHARLGPYDTIRVTAEIENGRLRVDVIDGGRGGGPPVAGGIRP
                     SPGAESGWGLYLVETLASRWGHGPGRYWFELEIGSSASPG"
     gene            complement(3417..3746)
                     /locus_tag="VIH70_14370"
     CDS             complement(3417..3746)
                     /locus_tag="VIH70_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117591.1"
                     /translation="MSDRAERVARNEATSREINEGIEQAHEGAPSDRYLRMVCECGMA
                     TCDRIIAITTTEYERIRSDPRWFAVLREHVAPDAEVVLEDHDRFVVVAKGEGTPAKVA
                     EQRDPRS"
     gene            complement(3757..4581)
                     /locus_tag="VIH70_14375"
     CDS             complement(3757..4581)
                     /locus_tag="VIH70_14375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEY8117592.1"
                     /translation="MGDPLSHERSRVLHALTAGGSVDPAAEADALLEASNAGAGPLEE
                     LLARRLAGEPLAWIIGWARFCDARVCVDPGVFVPRPQTEALARRAVALLPADGIAVDL
                     CAGSGAVAVVLGRAHPRATVIATDVDPAAVACARRNDVDALLGDLDEPLPTSLRRRVD
                     VVTAVVPYVPTEELHLLPRDVLAHEPHRALDGGPRGTVVLERAARAATRWLRPGGSLL
                     LELGGEQADELSTPWRRPACRRSGCIGTRRVATARSRRADPIRLRSHGSRTAGSPD"
     gene            complement(4589..4981)
                     /locus_tag="VIH70_14380"
     CDS             complement(4589..4981)
                     /locus_tag="VIH70_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8117593.1"
                     /translation="MSTSGSNASTTAIEPWLSVPDAGEALDFYRDAFGAGEAYRLDDD
                     DGRPVVARLSIDGAAFWIQGDADASPVAPGVGSVRMILSVDDPDALYERAVGAGATIV
                     APVHDEHGWRTGRLTDPFGHDWEVSRRL"
     gene            complement(5080..5616)
                     /locus_tag="VIH70_14385"
     CDS             complement(5080..5616)
                     /locus_tag="VIH70_14385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8117594.1"
                     /translation="MIRLVSSDALRPDEVAALREMFDEAWSDDAEGFTEQDWSHAVGG
                     IHVIAEADGRVVAHASVVQRELHSAGHRLRTGYVEAVATRPSRQREGLGSLVIGEVGE
                     LIDRTYPLGALATGVVGFYERLGWVAWEGPTSVRTAAGLVRTAEEDGNVFVRLTPTSP
                     ELDLSAPISCDWRPGDVW"
     gene            complement(5636..6496)
                     /locus_tag="VIH70_14390"
     CDS             complement(5636..6496)
                     /locus_tag="VIH70_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8117595.1"
                     /translation="MTPQRSSPTDQRDRERKRVSRLLKSHAPPDWEARFRATELLDAV
                     IDGEPIQMFVMAADIRESTTLMKESVKFERFAAIMDKFVSSVRLGIRRSGGWFDRFTG
                     DGFLAYWIVQSAEPDEYEERFVQTAGDIAHTANILIDLFHRRVLEDFRSNSRNLSSGV
                     GLSMGLDAGPGYLVKIADELTIVGPPVVGSVRMVTAAALPKEIVANVFLGERLREGQD
                     GVYEALGVRVNREFRPTKEYPRGQEVYALTFEQDGSELGTPNEDADEEAARHVVDVSD
                     ATGQGDGKVS"
BASE COUNT         1013 a         2429 c         2286 g          901 t
ORIGIN      
        1 aagcgtccag gaacgagtca ccgccccgtt cgatcccgtc cacgaacgcg gggctccaca
       61 ggatcagggc atagcccagc agtgcgaggg acacccaagc acctagcagc acgaagaaga
      121 agaagggcgc cgccccggcc aggacccgct cgcgagcctg gatcgtcttg cggcgcacgc
      181 cgacccatct ccacggacgc cacagcggcc ggaacaggat ccggctcagg cgggaccggc
      241 cgcgcgccgc gcggggcatc acgagggtcc ggaacacgtc gagcaacgtc cacaggagga
      301 tcacgatgcc cgccgggagc gcgatccact gcatcgccgc agcctaccgt ggcacgtcgt
      361 ggcgtttccg gggcagacgg tggccttgaa cccgtctcgt tcctcgggcg cgacccgccg
      421 atctcagccc gtctcgggga gacacacggt cacatcgacg gcggtggacg tgacgacgct
      481 cgatcctgcg ctcgggtgct gctcgatgat cgtgccgggg ggctgctcga gcgaggctcc
      541 agggatgtcg tactcggggc acgcgcccac ggagacgtca ccgagcgcga gatcggcggc
      601 ctcgagccgg agccgcaacg cgtcgagctg gggatcgacc cgcgtgccga tgagccgggg
      661 aacggtcacc tcggcgtcgg acctcgacgc gaccgcggcg gccccgacga cgaggaccac
      721 gccgagcaca ccgaacagca tccgtcggcg gaagaacgac gcgagcttcg gtggctcgcc
      781 gccttggccc gacggatcgg tgctcgcgcc cgggctctcg acggtatccg cggtttcacc
      841 gcccggcatg agcggtcccc cgaacgccga ccacgatcgg aacgcgatcg accacacccg
      901 caccctctcg cgatggatca cgagggtcaa ccgcgaggat cttccgtcta gcctccggcc
      961 gcctcgatca ccagatcggt cgccaggccg agcaggaacc cgccgagcac tccccacagc
     1021 gtgaggatcc acgaaaggcg ccggccgctc gcgaacagct cgccgatcac gtacaggatc
     1081 gctcccgctg cgagcgccag gaagccgacg aagacatact cggagctgaa cgacgttccg
     1141 acgatcgtcc cgaggaaggt cggaccaccc gcgacgaggc cggcgaccag gagccacccc
     1201 cacgtcgcct ggaccccagc accggcgagc ggccccacga tcccgaaccc ctcggtggcg
     1261 ttgtgaagcg cgaaaccgac cacgagcagg accgcgagcg agatctcgtt cgcgttggcc
     1321 gcctgcccga tcgcgagccc ctcggagaag ttgtgcaggc cgatcccgac cgcgatcgtc
     1381 atgccgaggc gcagcgcctg gcgatccggc gtcaggccga tctcggcgac ggccatcgcg
     1441 ccgggcccga ggctcgcccc gcgcgctcgg tggagggtga gccggaagaa gtacgggagg
     1501 ctgaggagcc cgacgccgag gcccgcgacg tagacgagac cgacggcgaa cagttcgccc
     1561 caggccccgt cgaccaccgc ttcctcgagc ggttcggtcg cgtgcgcgag cacgtcgaac
     1621 aggaggaaga ccaggatgcc cgccgacaga ccgctgagga aggtcttgag cccgaggctc
     1681 ccgcccttca gacggccgag cgggagcccg aggaagatcg tgaagcccgc caccgctccc
     1741 agggcgatcg tctgccatgt gctcatccga ccgccctccg agtctcgcgg ctcgaatccg
     1801 accttcgcag tcgagaactc taggcttaga cgcgtcgaag cgtcaaggca cggccccgcg
     1861 aacgccggcc cggctacgat tcgcgcatgg ctcgccccgc cgcgcccgca cccaagccat
     1921 ccgccccgct cgagcgcgcg gtccgctcgg agacggtcaa gcactacgcc gagacgatct
     1981 tctacatccg gcaggaggag ggacgggtcc gcccggggcg tctcgccgag tggctggggg
     2041 tgagcgcgcc cacggtcacg gtgacgctgc aacgcctggc ccgcgacggc ttcgtcgaga
     2101 tcgcgggcga ccggagcgtc accctcaccc cgacgggcga ggacctggcc gccaagatcg
     2161 tccgcgtgca ccggctgctc gaacggtggc tgaccgacgt cctggggctc gattgggcgg
     2221 ccgccgacga ggaggcgcag cgtctggcct ccggggtgtc ggagctggtg gcggaccggc
     2281 tcgacgagac gctcgggtac cccgccacct gcccgcacgg caacgtcgtc cccggccgcg
     2341 aacccccgta cggagagctc gtcgccctct cggacctcca gccgggcgtg ggggcacgcg
     2401 tgcgccgcat ctcggaggtt gccgagcacg acgcccccga tctcctccgg gagctcgatg
     2461 cgtacggact ggtgaccggc gcccggatcg aggtaagcgg ggcggacgcg agcgtgcagg
     2521 cgatcccggt cgacgtcggc ggcgtgacca gaccgatcgg cacgaacgtc ggccgactca
     2581 tctgggtcga gccggagtcc tccgggaagg gatcgaccgc gaagccgcgg cgcgcgccgt
     2641 ccgaccgctg acggtctacg aggccgcggg tgtcgccccg atcgcgcccg ggctctccca
     2701 cccagaagac ggcgagcagg gaggggatga cccgcgtcag cagagcgaag aacttccatg
     2761 cgatgggcac tctgatcgag gtgggtgttc ggggtttcgt ctccatggtc gctcgatcgt
     2821 ccgggtgttg ctcgggcatg ggcaagccag tcctccgagt acctgcatcg gcaccccctc
     2881 ctcgggcttg agcacgtcgc gcttcgaggg ctccacgagc ccgaccagaa caggtccgcg
     2941 ggcactcgac acccacgtgt tggatggtgc cagaggggga aggagttcta gatgcaggtc
     3001 cagggccggc gatcggcaga ggggggcggg cggtgcctgg atctcgagat gccctcgcta
     3061 tcgggcgccc tgacggacct gcggtcggcc ttcgccgagc tccccgtccc tgcctccgcg
     3121 ctcgacgacg ctcggctcct ggtgagcgag ctcgtcgcga acagcatgcg tcacgcgcgg
     3181 ctcggcccgt acgacacgat ccgggtgacg gcggagatcg agaacggaag gctccgggtc
     3241 gacgtgatcg acgggggtcg cggcggcggg ccgccggtgg ccggaggcat ccgtccgagc
     3301 ccgggggcgg aatcggggtg gggtctgtac ctggtcgaga ccctcgcgag caggtggggc
     3361 cacggcccgg gcaggtactg gttcgagctc gagatcggta gctccgccag ccccggctag
     3421 ctcctcgggt cccgctgctc ggcgaccttc gcgggcgtcc cttcgccctt tgccacgacg
     3481 acgaaccggt catgatcctc gaggacgacc tcggcgtcgg gggcgacgtg ttcgcggagc
     3541 accgcgaacc accttggatc gctccggatc cgttcgtatt cggtcgtggt gatcgcgatg
     3601 atgcggtcgc atgtggccat gccgcattca cacaccatcc ggaggtaccg gtctgacgga
     3661 gctccctcgt gggcttgctc gatcccctcg ttgatctccc tcgaggtggc ctcgttgcgc
     3721 gcgacccgtt cggcccgatc gctcactgcc cggactctag tccggggaac ccgccgttcg
     3781 cgacccgtgg ctccgaaggc ggatcgggtc ggcgcgcctc gatcgcgcgg tcgcgaccct
     3841 cctcgtcccg atgcacccgg atcgccgaca ggccggccgc cgccatggtg tggagagctc
     3901 gtccgcctgc tcgccgccga gctccagcag caggcttccg cccggccgga gccagcgcgt
     3961 tgcggcgcgg gcggctcgct cgagcaccac ggtcccgcgc ggcccgccgt cgagcgcgcg
     4021 atgcggctcg tgcgcgagga cgtcgcgcgg gaggagatgg agctcctcgg tcggcacgta
     4081 ggggacgacg gcggtcacga cgtcgacgcg ccggcgcagc gaggtcggca gcggctcatc
     4141 gagatcgccc aggagcgcgt ccacgtcgtt ccggcgggca catgcgacgg cggcgggatc
     4201 gacgtccgtg gcgatcacgg tcgcccgcgg gtgcgccctg ccgaggacga cggcaaccgc
     4261 cccgctgccg gcgcacaggt cgaccgcgat cccgtccgcc ggcagcagcg ccaccgcccg
     4321 gcgggccagc gcctcggtct gcgggcgggg cacgaacacg cccgggtcga cgcatacgcg
     4381 agcgtcgcag aagcgcgccc aaccgatgat ccacgcgagc ggctctccgg cgagccggcg
     4441 agcgagcagc tcctcgaggg gtccggcccc cgcgttcgag gcttcgagca gcgcgtcggc
     4501 ctcagcggcg gggtcgacgg agccacccgc cgtgagcgcg tggagaacgc gcgaccgctc
     4561 gtggctcagg gggtcgccca cggcccagtc agagtcgtct gctcacttcc cagtcgtgcc
     4621 cgaacggatc cgtcagccgt cccgtgcgcc acccatgctc gtcgtggacc ggtgccacga
     4681 tggtggctcc ggcgcccacc gcccgctcgt acagggcatc cgggtcatcg accgacagga
     4741 tcatcctgac cgaccccaca ccgggtgcga ccgggctcgc gtcggcgtcg ccctggatcc
     4801 agaacgccgc cccgtcgatc gagagtcgcg cgacgaccgg tcgcccgtcg tcgtcgtcca
     4861 gtcgataagc ctcgcccgcg ccgaacgcgt cccggtagaa gtccaacgcc tccccggcat
     4921 cgggaactga cagccagggt tcgatcgcgg tggtcgatgc gttcgacccg gaagtgctca
     4981 tgggcgtccc cctccagcgg tcggcgtcat cgggtcattc tctctcagcc gaccgcttca
     5041 tcgacgtccg ggcaccgact tccgatgagg agggccgagc taccagacgt ccccgggccg
     5101 ccagtcgcag ctgatcggcg ccgagaggtc gagctccgga gacgtcgggg tcagacggac
     5161 gaagacgttc ccgtcctcct cggccgttcg aacgagcccg gccgccgtcc ggacggaggt
     5221 cggaccctcc cacgccaccc atccgagccg ctcgtagaac ccgacgacgc ccgtcgccag
     5281 ggctcccagc ggataggtcc gatcgatgag ctcgccgacc tcaccgatca cgagcgaccc
     5341 gagaccctcc cgctgacggc tcgggcgcgt cgcgacggcc tcgacgtacc cggtccgcag
     5401 gcggtggccg gcggagtgga gctcccgttg gaccacggag gcgtgcgcca ccacgcgccc
     5461 gtccgcctcg gcgatcacgt ggatccctcc gaccgcgtgg ctccagtcct gttcggtgaa
     5521 cccctcggcg tcgtcgctcc aggcctcgtc gaacatctcg cggagggcgg cgacctcgtc
     5581 cggacgaagt gcgtcgctgg agaccagccg gatcacgggc cgggcccccg tctcctcagg
     5641 agaccttgcc gtcgccctgc cccgtcgcat cggagacgtc gaccacgtgc cgcgcggcct
     5701 cctcgtcggc gtcctcgttc ggcgtcccga gctcggatcc gtcctgctcg aacgtcaggg
     5761 cgtagacctc ctggcccctc gggtactcct tggtcgggcg gaactctcgg ttcaccctga
     5821 cgccgagcgc ctcgtacacg ccgtcctgcc cctcccgcag gcgctcgccg aggaagacgt
     5881 tcgcgacgat ctccttcggc agcgcggcgg cggtcaccat ccgcaccgag ccgacgaccg
     5941 gcgggccgac gatcgtgagc tcgtccgcga tcttcacgag gtagccgggc ccggcgtcca
     6001 gccccatcga gagcccgact cccgacgaca ggttccgcga gttgctgcgg aagtcctcga
     6061 ggacccgacg gtggaacagg tcgatgagga tgttcgcggt gtgcgcgatg tcgcccgcgg
     6121 tctggacgaa gcgctcctcg tactcgtccg gctccgcgga ctgcacgatc cagtacgcga
     6181 ggaacccgtc gccggtgaac ctgtcgaacc acccgcccga acgccggatc ccgagccgca
     6241 ccgagctcac gaacttgtcc atgatcgccg cgaaccgctc gaacttcacc gactccttca
     6301 tgagggtcgt ggactcgcgg atatcggcgg ccatcacgaa catctggatc ggctcgccgt
     6361 cgatcacggc atcgagcagc tcggtggcgc ggaaccgcgc ctcccagtcg ggcggcgcgt
     6421 gcgacttcag caggcgcgag acgcgcttgc gttctcggtc gcgttgatcg gtcggggacg
     6481 atcgttgggg cgtcaccgca cctccgatga cgtggcggga agggtcgcca gtctagcccg
     6541 aaccgttgcg gtcgccgccg aacacgcggg tgagccagga cgaccacgcg gcctcggccc
     6601 gccctcggtc cacgccctcg gcgaacaga
//
